LOCUS       DAOSOM010000001       151000 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_74_length_151000_cov_6.410978, whole genome shotgun sequence.
ACCESSION   DAOSOM010000001 DAOSOM010000000
VERSION     DAOSOM010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 151000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 151000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..151000
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74_length_151000_cov_6.410978"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PLF14_00005"
     CDS             complement(<1..398)
                     /locus_tag="PLF14_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT69717.1"
                     /translation="MKKKKIFKILYTFISVIFIASAIITTIMILSFDMQYACPKFGNH
                     ILVSVNDSELSPKILKGSALLSDLKYDKVEKGDYVIITTKSMAGIQYSVRHLESVEDE
                     YYLVSGTEGRGPFYVNKSSVIAVSKMQIYGL"
     gene            complement(411..1274)
                     /locus_tag="PLF14_00010"
     CDS             complement(411..1274)
                     /locus_tag="PLF14_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT69718.1"
                     /translation="MKRKLLSAGIRLALGLVAVAIVSYWWFSDAWFAQSEKVQANGVE
                     LSAMVPLTVYISGSQDERLSQQLTFSMANFEELGFSSLYDYGVNIVLRPSSSFDGKNF
                     WFARKVQTDGSAITDPETGTKYAAVGQNDKKFCMEKTVYLVTTTEAHSDITALDCFVS
                     KVAISGLTDNELYKAARISLSVTDDLGVETTYIYRYAADGTDTSGAALPVTGINTISG
                     SDPSIAMGIYESGQAGALPFKLICANSGTISVRELTIRIWFEGENAYAVRAHAGGGFA
                     FELEFTLVDPA"
     regulatory      complement(1322..1404)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1554..2381)
                     /gene="amrS"
                     /locus_tag="PLF14_00015"
     CDS             complement(1554..2381)
                     /gene="amrS"
                     /locus_tag="PLF14_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOT69719.1"
                     /translation="MKIKCPICPNSCMLSEGQVGLCRARINKDGKIICENYGKLTAIA
                     LDPIEKKPLYRFFPGKMILSVGSYGCNLRCPFCQNYDISMADDSKVGTYQTQPDYLVD
                     VALSMKDRNNIGIAYTYNEPLISYEYVKDCSVLAREKGLKNVLVTNGYVCREPLDNLL
                     LYIDAMNIDLKAFNEDFYRKIGGDLETVKDTIVTASKSCHVEVTTLVIPGENDSVEEI
                     TSASRWIASIDENIPYHISAFYPRYKYSDKRPTSRKSVYDLVEEAKKYLKYVYPGNC"
     gene            complement(2378..3706)
                     /gene="amrA"
                     /locus_tag="PLF14_00020"
     CDS             complement(2378..3706)
                     /gene="amrA"
                     /locus_tag="PLF14_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006444396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOT69720.1"
                     /translation="MSVAAAFIFPHPPLILPKIGKGEEKKIQKTIDSYKEACKTIAQL
                     QPDTIVLASSHCAMYSDYLHIADGEYFESDMGRFGAASISVRTEYDKQFRDKLLKIIS
                     EQNIPAGTQGREIPGMDHASVIPLVFLNEVYRDYKLIRMGISLLPFEIHYKLGMCVAK
                     AAELLGRRVVFIASGDLSHKLKVTGPYGYSKQGEQYDQMVTEAMAKGNFAKFMDFSEE
                     FLEKAAECGHRTFVIMAGALDKKTIEPKLLSYENTFGVGYAVASFLVEQKEKEVRLDM
                     YVALAKASVEYYVKNKRMLKMPPDLPAELTAKRAGVFVTLKKDGDLRGCIGTISPVTE
                     NIAEEIIRNAVSSCSHDPRFFPVREEELDALEYSVDVLKEAERIDSIDQLDVNRYGVI
                     VTSGYRRGLLLPNLEGVDTVQQQLAIALSKAGIASGEKFEMERFEVVRHK"
     gene            3885..4970
                     /locus_tag="PLF14_00025"
     CDS             3885..4970
                     /locus_tag="PLF14_00025"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOT69721.1"
                     /translation="MKKIRLPIILLILSVMISFAFSGCNSKNQDFTFSRDLDNNGYWR
                     GVKALKYVELCDYKGITVPKDIHTVSDDELQSAIESLLSEQASSIKVTDRAVVYGDTL
                     NIDYVGRIDGVAFEGGSTGGAGTEVTIGVTSYIDDFLEQLVGHSPGETFDIEVTFPDN
                     YGKEELNGKDAVFTVTINHIVESVIPELTDAFINSTFSQTKGWTTVEQMKADVYKDIQ
                     SAKIADYIQEYLHANSTVKEIPKIITDYQDTAVIKYYQDYADYYQVTLEEFLQQYLNV
                     ENQQALLEQYKEDNINVSKMHLITQAIAEKEKIKATADDVTRYFIDYMNTDDYSQYEE
                     VYGLNYLKMTVLVQMVIDYVQASVVLE"
     gene            complement(4976..5533)
                     /locus_tag="PLF14_00030"
     CDS             complement(4976..5533)
                     /locus_tag="PLF14_00030"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HOT69722.1"
                     /translation="MTEKEKSHAGMLYQPGDPELVADRDVTVQKLYEYNRIHPLDREA
                     KKEAIKGILGKTGKNCVVEQPLFCTYGYNTTVGDNFFLNVNCRLMDSGKITIGNNVFI
                     APDVCIITEVHSMIVEERIQGLEYTYPVTIGDNVWISTGAHILPGVTIGDNSVIGAGS
                     VVTKDIPANCLAVGNPCRVIRKLNE"
     gene            complement(5558..7120)
                     /locus_tag="PLF14_00035"
     CDS             complement(5558..7120)
                     /locus_tag="PLF14_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT69723.1"
                     /translation="MKRIFRRGEQEVQPKSTKIISIILLFFMVICLSSCDKVPQVTEY
                     CSHEVVIDKAVPATCTETGLTEGSHCSLCNTVLVKQQVIPAEGHSSEEYDFDIENHYG
                     YCLKCGNRFTEKHDMDPETKKCSKCDFETVTPPPFPSGYITKEHTTIETEHFICEIDA
                     DTYIIDNLEYYLETLFDALEVVSGLSFNTAELGKILVEVKKAQAVDGYVSETHIAYAY
                     FNRIVISTGDLFISGGHAIAHELSHILQNHNCGWNFRRILPEGFAVYTTYKVTKYMEE
                     NYPDIAYALEAAENNMRNEKISAEGYKELYSKPLSYWQENHLPHSMSSDYAIGFRLMK
                     YLDSVYNDYSSWITEYQKVNPFTPDVSDDRLDIREINRVLPMTYGEYVEANFYTWLKR
                     NEGFFEEKPDNTTDRTHLSYINIYPKYNYRLSAMCFEGFEYQDIYFNIDQLRRYITEY
                     KGDKLGNFRFFFGALEPTTIKLYDSEGNLIAKHFSETAVGTYSLEGVGFIQFVGRGTI
                     MRFNISGFASIE"
     gene            complement(7217..13750)
                     /locus_tag="PLF14_00040"
     CDS             complement(7217..13750)
                     /locus_tag="PLF14_00040"
                     /inference="COORDINATES: protein motif:HMM:NF021028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOT69724.1"
                     /translation="MFKRFISIILVLSVILALIPAGIVSAADDDLTEISNQYIKVIVN
                     NKNGGYVISTLEGDILKKSDNNAFLTHRGEYYDTSFTSFKVDNDEYVFGEKYGFLGLQ
                     SNYAVTEKDVNGAFIKSTWSIGDFVFEQVITLINSEASEQLGTAMISYNVKNESAAAK
                     SVQSRVLIDTQLGSNDYGYYEIPNQHLGQGYEYFEFERTWDSKADPTIKMPADYFVRD
                     NPYSSTIVAYGVNSVFTEQKPYKMTFAHWANIAQTVFDYEPVTTLNFTNDANKKKTAD
                     SAAALYYDLGVIEPGKSKTFSTYYGVTANLKNKDNKIIINTTAPSKLEFTDETRTAYV
                     GTGNVDDIVRVNVNVTNPLYAEKDYKELAVVVYAIGFTPLRITTGGSWIEYSNISPMY
                     SYITDFKSGDNTVTYFDFKFTPQERAQLGTFIIKVFDIDEDVNELGSYAEDYCLGTTE
                     NHIIIPGRDSTLPTITLAGLAPDIVYNQETRYITVTGQGMSFFRSDLLEKIVFVGDNG
                     RNYEVSPQNIIFDQADPEKISIMLTGHMYMGRYQLHFKWKDDKTGQGVLQGVPSDFTS
                     DAMYVQMSGDLKYKNDFYGIVTVQRAGNNKYKVVPYKDEDALRDAQIPFNDLLLTFKG
                     PLLQEQSNKNLYRLSGQGQNITINYALWYHGSDLTIEQKDNGTVEVLMDGKIRTMGAN
                     TMVRDGTAVFRLKAGVEYLVPLYDERGIVQEDGSLAVGQDFIELKWDNAMDVLTTIGG
                     FLIDLKYGVLGRMQYEDGTITNVISFGGGLDLGFITPGGAKVARENRNAAKNWKTKGS
                     EVEYKDDDDMYSFGLDFDDATGEIKTQTYEEDIEPETKEAKRISAGVHVYDVLFGGKK
                     PGYLGINMEAYFSLPQIVAFLPKKIGANLAINTVNGYKVGIDAEVKAFNVEIEFALVI
                     ISAPNGAPIPDKLFLSVGGFEPGYNIDGLTVFWLTGAGGGIDNLYDTIYGKDGIPPLT
                     ILLNIQFDISKILTGSADLELSLRYIKITFDDLSIKKIKDAKFIDGGSIAIGWYPNFF
                     FNFTGAVTFQQIMTGRLTISAAAGKNIDDFVEFVLRVSIGLPKWIPIVGGMELASAEL
                     GGGSEKVWGSIELLQLIKVGFIYYWGGKVEFTAGDIDGGENFAAMSSTDDPGVRRTKM
                     MFNRMISPNEVGKDPKTGEVQYAAVGTNLSYAAGSTAVDDFEQRLAAFNNINNLRNTG
                     MRRNLRGPQALNTEIYTNTERTSHLIKFGEACDYILTVSRVDGSDLTVQDLQACMTVK
                     KGNAVYPLSYYIAPPHNASDDVKAAALKNANVNVSGKAANIVIPKADATENFLIEFSD
                     NNPYDVSALKVNPIAKLTTYNATVNGNVLNVTWNGEGISDTATVVISAHDGNKDNSII
                     LNPRVIKANEYSAAITIPEKMSSGEYNIVIALSEENVSYDTYDVGKVTITNSKAPGAP
                     DSATMENCGNDKLRINVATAQDNFDGYLVEVYEDGLLADTGLYFAKGEEIIVGGCYMM
                     PVLDENGNPIPIGGNDKYGNPLIQTVPVGYTPGKSYVAKVRLCNIEKDADGNDLYHCS
                     AYATTGSVVLKGETPPVITIGYDKATGEIKVTSDVPVVGEMYINAATQNGEWYEYKEM
                     STEISQSVNLPDGEHTIEFHATDADGDKSITTEIINIDTTPPVILLALPQNGSFFDGD
                     SVTVVATADEDATYTFKINGKVVAPEEDKIFTDRLMKCTLPLGEAKNLSRIQLDITGR
                     DAAGNEVFKTLTLLNRKLSEIKSISIECPDKPVIKGKLTLAEGETAQLKVYGKTDNEK
                     IDITNLPGITLKVMSGTAVSMDRATVTAGIVGQSLIYAKFALGGNDALYDGIVVEVSD
                     EALVFTGLQELLAEAKQIKKDGLTDNTRNELENAINSAEQIIVTEGIHQSDIDNASTA
                     LSNALAKVKAEISANSTPYYYISFNPNGGSNVRSQRIDYGKTVAEPTNPVKAGYTFEG
                     WYTDTTLKTRYNFAKEVTSAFTLYAKWTKNEETQWYDQFTDVDENDWFYEAVKFVYET
                     GLMRGISDKEFAPELLATRAMFVTVIHRMESEPESQPCPFTDVPKGSYYENAVAWAAA
                     NGIVNGVSEDSFAPNNNITREQMATMIYRYAKYKGLDTSVVEETNIASYDDYNSISQY
                     AIEPAKYVVGTKIMIGRTETTFNPLEYTTRAEMATVLMRMLDKMN"
     gene            complement(13771..20610)
                     /locus_tag="PLF14_00045"
     CDS             complement(13771..20610)
                     /locus_tag="PLF14_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT69725.1"
                     /translation="MDSGLYAAMLVGRDIKGPKINTIRVTADAAGTREVTNGVVSLET
                     IAELDAKRTIYFQVEWDEPVLYKKQKEENANIENLKLFVQVMGIAGSGQIVEANFLSY
                     TSTDKTPRPVMTFEYQLPDPYSYDPKVPTDENGCYYDFKCVNVSIDENKDLWECIFDL
                     SGNKFASVNGIQPDATKNKPVSATFSNYACVDLRPFGITAFEIIGERNKAFLKAFQNI
                     EINLTLNKAPFKSFTEDVPLTDYPTITLNLKNKNNQYLTLSPKGVETGKQKSHIVKYG
                     FRLTSYDDDYNILDGDFLKITSINFNNISRFKDRGGGTFMTYIPQDNGVLQPTNIPPL
                     PAKDKLKLTPSPNGEFKVDFIAPVINTTVTDEGSGIYGIKALVDDMSLDGAKATITIK
                     VAGKSAPTALLQYQSTTSESYQDIPGAWRKGDAGTMSASFVAPVSKEGGVTAAYAYFK
                     LPNDLETNKVDITVAVTDNAGNRGTNKYTLCSPGWDGFDRLAPRSFAYFWSMTEQVIL
                     KTEDMDTATEYRYGYSLNDQDAPESYSDWIPLTVGEEALIPPPPNESLTPQEINERVV
                     WIEIKDSNGNTAIKKATAQYDRTYVDIEINSADTQTTYEAGHYPTVDFTVKHAKFYWY
                     RWAERPARSVENTESLIANDAGQIAIVKSQGANLTYGNESVQRTLTLTDNAIVQKVDP
                     DTEKLKYDSTTPGNTTRPIMLVIIAEDDDGKRVVKTVEFNTVYAPPMVSAVQTRFSTN
                     DNSGKRVDYLRADGKLIWPTDDDSQWPEYSKGQPVNTPNLFGIAQAEFVISKDPGTGF
                     DRIDFANSSITLEKVLRSESRITSGHEVERTVLETWKLNELTLINTSEGNKLAIVDFD
                     PNSEYISHQYYEYKDGECYTAYYEFVCNLSYTNNLESTKTPIAHYLFCDMPTGILFDI
                     NYDIGYPYNIYGKYPTDNVEAVFDNDGNDITDNIPVYPINTLPHEEDGNNMYIRFSVP
                     GGDYGYQGSAYYRSPMLNMVDPANTYKLAVHIGTDANNLSDIVPIKTNWEYDFYAAVR
                     SDNYAIPLELFGNDDEVREVTLYYKFVNPERGSESPVYVLKLRRDNVAPVFDVFVSET
                     NQMVKEVLVKLNSVYDVQTGADGTVYEDTPEHVLRSEMWFEAWRDITEEEFDSIQGEE
                     YDRFNNRDGNKKREPGGRYYARVRPDQNGFYHFITNGYIYFSLEDYAGNYFKVLVNGK
                     EVVLPTEGGETDMNCYHITNVDCEPPAFVTDPVFAAGDGKFTLTAKADQSVNRVYLKF
                     DDTYSEYITGSDAPAGIALYDIKRVPGLLSGAFNATTGDINAEIYVKYSDTVKLTSVT
                     VIIEDAAGNRTESPYVFGEPIAGKKAEITNPQNADGLPVYNYGETLTFNVPVKIVGLS
                     GNYATSHNNLAIYTNGKTSISYINLFGDTETKDIYANIFESAFAHTLKFYSNNVEISP
                     DTPVNTDVTVKIDTSKTSNLTVEGGISEIKAVTNGLLTYSLTNTELGQTETFSIPIVN
                     IDRTAPDASVSLELETETDIQTGASLVYSATYVILGYSEDDVTLIANADGSAPSYVTF
                     DANSVNRTYTFRFRDAAGNEGSYTVDASNIVFTERTDKKIAGYRLTYTVPSDNGFSTL
                     GQFKKGEEITGLGLVNMAVSVRIETLNAAGDVVSSIITANDGLPQGVSVYNKQKLVMF
                     TTESDEDRVVNLTLTGTGTDNDFTVPVKLCANTMDLTAPSGTVYYKADGLNVKAYLVT
                     QDTDIRDDGISVTGAKTDGTPFELKQDVDGYYVELDMNGTGKFVLMDKAGNVGNVLIA
                     VLTIDTEAPKVVLEGWQSIFDANASDKELLRQLLETPTNSSIKVFITCNEMLKNTEVT
                     AYENDETTNELQPTDDYVTAVTSGPTVVVEFKQNCRAKLVIYDLRGNGLTLWRPDDGP
                     IASIDKETPKPAAGYPERNIIDNKEEIKYVFANGEPVMLLQNHENGYKNQHTITFEEN
                     GPQTLNFADRAGNVYTDHLVISQIDDLAPNVKVSLDFVGEGAVLSPKDSYKAGNVYTS
                     KDVRILLNVTDEGTNNEGLVVTAKTRLGASLAVNKEAVTANEKDYNYNFVVTENGSYE
                     VTATDKWGNKNVFWTNVSVIDREGPTVRVVGNNVIVLAGTSAEDAKESLLELTTAFDT
                     QSGANAPLSDKMQNVNDGVTLAVDFSNVNFNVANKYIATITATDRLGNVTNKNCTVSV
                     VENLFTINLDGMKIYAFDTVTGAKGKHTVDDTDETTKYYYSAGYKTLAEMKYAKAFKP
                     EIGFYATQDGYYTIMAKDVNRNMYLIYLFIY"
     gene            complement(20692..21333)
                     /locus_tag="PLF14_00050"
     CDS             complement(20692..21333)
                     /locus_tag="PLF14_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT69726.1"
                     /translation="MRKRFIAFILTLAMIAGMAGFYPGIIVSAEEEDTTKYNKYGHAI
                     TYSAHETLHTGGKQVKFFDAGYTAWGTAPEGTANDEAKSMEWLSSNGYLLNHFGNRGS
                     ADNSKFGSSFNSNGEWQTKLKGPSNVKEWKVRVWFDGLTKQEERDKGNISGMYDKGDI
                     EYFFAWDVKTVQTKWWGQDGKSRDDGYTQIFGEEIHSWGNHTTGYFKEDNSGP"
     gene            complement(21350..21727)
                     /locus_tag="PLF14_00055"
     CDS             complement(21350..21727)
                     /locus_tag="PLF14_00055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT69727.1"
                     /translation="MKILIVCRDAELAALSGKSLKRNGYDVTCLSDISATESLIKQKN
                     IQLVVADIETDEKECLKFCETVKKISNAPKLLFIGRSDEEESAILNAGADDWIKKPYR
                     TPVFLARIAALMRQNGEDIRTES"
     gene            complement(21851..22552)
                     /locus_tag="PLF14_00060"
     CDS             complement(21851..22552)
                     /locus_tag="PLF14_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT69728.1"
                     /translation="MSNTEITILIVEDEPDILNINARLLKRRGYNVITASSAEESYRC
                     LSSVTPDLLILDIMLPDGSGYDICERFRSKCDNPVIFLTGKTRISDKVEGLRCGGDYY
                     LTKPYSFDELLAVADRLLTRHFEVKARQGQPESVTKCSITLDFPKRKAFVNGKDVSLT
                     AKEFSLLLYLMENEDKILSPDQLYEAVWGMPSTADTRTIRFHIGNLKKKIDTENADDF
                     DIISVYGKGYTFTTK"
     gene            complement(22545..23864)
                     /locus_tag="PLF14_00065"
     CDS             complement(22545..23864)
                     /locus_tag="PLF14_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005426718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT69729.1"
                     /translation="MKKLVTVNPRKKIKEMRMRFFDRELDFRVRLFNVLAIAGTAISL
                     AATVVNLITSMWVSAAVSGLLAVLSFALTYFTYKTGKYQTGYIITIIAIFMVFFPMLF
                     FTSGGYKGGTPALFIFAVLFTTLMLEGKWAILVSFAEIAEYVIIAVIAYKNPTLVTWF
                     ATEWEMLADIIFTTIAVCIACGTVIFFHLREYEAQRIKLSEQNEQLKHYSEAKSVFLT
                     TVAHEIKNPLNAINLYAHDTVELLDEKDPDINTMRANQNTIEKMVLRIDRIVVELMDT
                     VAIEQGRLSLDLAPVRLSHLLRETTEIYFNKNNTGENRLILKLDEKLPPIVADSARII
                     QVVTNLLSNSMQHTKNGFIMITLKGNSKSQIVSVTDNGEGMSEEIKSKVFGGYISVNK
                     EKWRHGIGLFVSRQIIEAHEGKVWIESELGKGTTVSFSLPYGEKTDE"
     gene            complement(23961..24614)
                     /locus_tag="PLF14_00070"
     CDS             complement(23961..24614)
                     /locus_tag="PLF14_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT69730.1"
                     /translation="MSRKSTINILAGCMILLSVMVPLIQFFRLYLHERYFSGSTWFVI
                     LSVLCMILAVLLFAKKSGWVFVILVLLICAYDTCEGIRVFKLDGMRELTSILEDYSRF
                     SSYFHYVVGPAIFSLISFVIPLLLVVSEISKKKNNKFVIILSVILFVIIVIFFFNAFY
                     GFISRLLRRHTVPLMSIISELLYLIVTIFRPIGLALGVFWYVNRSGKYDRGIYSLFR"
     gene            24816..24891
                     /locus_tag="PLF14_00075"
     tRNA            24816..24891
                     /locus_tag="PLF14_00075"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24849..24851,aa:Pro,seq:tgg)
     gene            complement(24880..25173)
                     /locus_tag="PLF14_00080"
     CDS             complement(24880..25173)
                     /locus_tag="PLF14_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT69731.1"
                     /translation="MHILDSNQTMPNLFQNIRNAFAHGSFLVRKHRVDKEFYYFLESR
                     NPENELINARLVLKASTLTKWIKVVKKVLKEQRSFLSGNSGTALSFAFFSWSE"
     gene            complement(25211..25771)
                     /locus_tag="PLF14_00085"
                     /pseudo
     CDS             complement(25211..25771)
                     /locus_tag="PLF14_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009644043.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            26060..27103
                     /locus_tag="PLF14_00090"
     CDS             26060..27103
                     /locus_tag="PLF14_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005965117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOT69732.1"
                     /translation="MRKFDYLKLMNLSLPVSIYHTIAQIHEYKGKQELYVVNYPDVLE
                     KMINIAKVQSTKYSNAIEGIYTNDTRLVELMNKKSEPRNRNEEEIAGYRHVLDMVHEN
                     YPYIQFNKSDILTLHNQLYSFSHVNHKGKFKTMDNTIVEVDSLGNRKVRFRPVSSFET
                     EKYFDEMVEAYRKAVEANIPPLILIPVLIHDFLCIHPFDDGNGRMSRILTLLLLYKFN
                     YFVGRYISIEMLIEDSKNSYYEELQNSSEKWHTGENDELPFIKYMLGIILKAYEECDN
                     RFKLIGDKTLSSPERVLAVIQKSLEPLSKGDLVMLCPDISQRTIERALKELKENEKIR
                     QVGSGRSTKYIKI"
     gene            complement(27198..28052)
                     /locus_tag="PLF14_00095"
     CDS             complement(27198..28052)
                     /locus_tag="PLF14_00095"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT69733.1"
                     /translation="MNTIWSDNVQGIQTLYLSRKLRFDDVFFCQYRDSFNIDKDAELK
                     ILEIGCGPGALAEAMHRWYPNAQITAIDRDSNFISFGRHNVAGVEFIEGDAEHLPFAD
                     NTFDVTISNTVQEHIEPNAFLSEQYRVLKPGGICLCLSTRKELSCIAPCLKITKEEEE
                     FWKSVTSEKILEEYGVCQYPMSEAELPASMEQHCFKDVTTNYAIINLTPDKPEYSVTM
                     GERIIKSMRQTNLESIAIASAHSTEYKRAIDAVKSKFDERLRLYGEGIKQWDTSVLII
                     MIIRGKKR"
     gene            complement(28185..28260)
                     /locus_tag="PLF14_00100"
     tRNA            complement(28185..28260)
                     /locus_tag="PLF14_00100"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(28225..28227),aa:Pro,seq:tgg)
     gene            complement(28361..30850)
                     /locus_tag="PLF14_00105"
     CDS             complement(28361..30850)
                     /locus_tag="PLF14_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOT69734.1"
                     /translation="MKNLHLVCNAHIDPVWMWNLDEGIAETLSTFRVCAEFCEEYDGF
                     IFNHNESLLYEWVERYEPALFKRIQKLVKEKKWHIMGGWFIQPDCNIPTGESFVRQIL
                     EGHNYFMEKFGTFPTTCINFDAFGHTRGLVDILKNSGYDSYLFCRPEPDMIDLPDEDF
                     LWEGFGGETIMCNRAFNSYESHRGEAHEKIKSWMDKNRHRKVGMVLWGIGNHGGGPSR
                     IDFKNIAQLAKKEKDFNIIHSTPEAYFAELDEKKLPVFKKTLNPRYIGCYTSQIRIKQ
                     LHRQLENELYTTEKMAAHASFLKLMKYPADRIRTATKDMLFMEFHDILPGTSIQSVED
                     YSIALAGHGLDELGKVKTQAFISLCSGQKKANEGEIPLLVYNPHPYEVPYIVECEYQL
                     ADQNKNRNIFANPVVYRNGKKLPCQVEKEDSNFNVDWRKKSVFYAMLKPCQINRFDVR
                     IEMIDAKPLPKVRQTKDMFIFKTKELLVHISKLTGTIDKYEVKGIDYLKKNAFLPVVV
                     KDDENSWAHKEKSFRNTEGFFKVMDKEEGNYFSGIIDENKIESVRAIEDGPVRTIIEA
                     VMKYDNSFICIRYHLPKRGTQVKVTVRVYWNEKMKMLKLSIPTTIENACYTGQQAYGS
                     EQLLTDGNENVSHKWNILYNKDYAVSVINDCTYGSDVLDGEIRISLLRSAGYAAGKSD
                     FSVRNKYIMPQDRFSPYMDQGERQYEFWLDASETTERFNKVEREAAVCNEKPMALSFF
                     PTGAGKRVRPFAILSDDVITMPVFKRSEDRKGYIIRLFNPTDTKKTTKLSLPLFRWER
                     EFTLDKYQVRSFRFDVKTKQVTETDLIERDI"
     gene            30987..31895
                     /locus_tag="PLF14_00110"
     CDS             30987..31895
                     /locus_tag="PLF14_00110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOT69735.1"
                     /translation="MKHLFIINPMSGSKKAAKFLPEIKKYFEGREDEYSIVYTQHATH
                     IKELCEEYAKKGGYRIYVAGGDGSVNQAANGVAGYDTPIGILPTGSGNDFIKNLTDYT
                     KLKDLVKRTVEGDVIKVDAIKLYDEELFGYCINILSMGVDAEAAYNSTQITRKYGITG
                     MLAYLLGFFRVLRQKKTKFDVKITIDGQVINDGYVMLAACTNGKYYGGGFIPVPHTSF
                     DDGIIDMCIAEARGLDFVFSVLPKYMKGKHVGVEGITFPKGKNISLESKDMLKVNIEG
                     EIIERRSIRMEIVPRLINVIKPKDLE"
     gene            complement(31901..32236)
                     /locus_tag="PLF14_00115"
     CDS             complement(31901..32236)
                     /locus_tag="PLF14_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT69736.1"
                     /translation="MEKNEDLGTRLENAGKRFFKKTKEFADTTKLSIKLKELDLDLDN
                     LYQIAGKAAYFGEDTKEIFTQITDKLEQIEEVKVDIADAKGMKICGNCKAEMPKEAVA
                     CMKCGEKFE"
     gene            complement(32263..32715)
                     /gene="mscL"
                     /locus_tag="PLF14_00120"
     CDS             complement(32263..32715)
                     /gene="mscL"
                     /locus_tag="PLF14_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005775701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOT69737.1"
                     /translation="MAKKNKTNLLSEFKAFITKGNVIDMAVGVIIGAAFGKIVSSFVN
                     DLLMPLIGLVMGKVNFNDLKWVLATDAEGNITSSVNYGTFLQNILDFLIIAACVFLLV
                     RFVALVRKKAEEAKQKNKEKEEVKAAPPAPSEEQVLLTEIRDLLKEKK"
     gene            33131..33688
                     /locus_tag="PLF14_00125"
     CDS             33131..33688
                     /locus_tag="PLF14_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009643967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOT69738.1"
                     /translation="MDYYDINIAGVQRRLPLCKVTDNLYIAAFVVFGDIELTIACAKR
                     LLELAPEYDYTITAESKGIPIVHEMARQSGVNKYFLARKAPKLYMTGVFEVDVKSITT
                     SGLQKLYLDTADAEFMKGKRILIVDDVISTGGSLRAVEELVLQAGGIVAGKMAVLAEG
                     DAIYRNDITYIAPLPLFNPDGTIKR"
     gene            33685..34773
                     /locus_tag="PLF14_00130"
     CDS             33685..34773
                     /locus_tag="PLF14_00130"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOT69739.1"
                     /translation="MTGKNKNSTTVNAKRKAVIIVLSLLLLLSGCLKESSLYITEKNG
                     MYTTSFIAMDTVMSVSVISKNAEKELHEAYKRILAYDVAFSAHSQNSEVSKVNNSSGK
                     DIAISDELKEQISTALKVSKMTDGAFDITVFPLMELWGFYDKNHTVPDEASIKDILKL
                     VGYDKIGLEGNMLNLQSGTKIDLGAVTKGFAGQKTAAMLIDSGVKSGMLSLGGNIQLF
                     GLNPDKSVWNIAVQDPKDEHSYIGIIHDTDCSIITSGCYQRYFEKDNKIYHHILNPHT
                     GYPADSGILSVTVVANDGTYADALSTALFVTGLDKAVEIWREYGDFEAIFVTEDKHVY
                     ITEGLKDKFTPYSCQDKTDTYLYIYINK"
     gene            complement(34774..36009)
                     /locus_tag="PLF14_00135"
     CDS             complement(34774..36009)
                     /locus_tag="PLF14_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT69740.1"
                     /translation="MKPELLKLREYAKQYAEIALSDSNLAIPEQYRKLNSLQQVRPPV
                     LVFEVPWGELEDQEKLRLECETDLYRGIERAVKRALYQFEYFRGDYAIHPYYKVRIRT
                     KGTGIGLTVQEVKIGSATGTDISAHEYHDILPDEESLEKITMPTMQVDEQATQRALEA
                     AHDVFDGIMDVGLGGYQLIFNMWDEIPRYHGVENCLIDIYERPDFIHKMMDKFTRYHE
                     ETVSLYEKLNILETDAYYLHCTPACTYELPVKDVAEETVSAKDVWGRGMAQIFAVVSP
                     DMHKEFDIKYMKRLFDRCGLTYYGCCEPLHNKIDILTEFDNLRRISVTPWADVDIAAD
                     AMGNRYVLSAKSNPAYVSQAVFDPQPVIEETKRILLACKRNNTPCEFVLKDISTVNRN
                     VNHLSEWVKTVSQTVDRYW"
     gene            36228..37025
                     /locus_tag="PLF14_00140"
     CDS             36228..37025
                     /locus_tag="PLF14_00140"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT69741.1"
                     /translation="MIIDFHTHIFPDTLAKKAKETLLRNVNYRYEPATDLTKAGLIET
                     MDKWCIDISVNQPVITKRTQLQSTNLWAQSVCDDRIISFGGIFPHTEDYKKDIDFVCG
                     LGLKGIKFHVEYQAFGVDDPKALRIYDYALSKGLIIVHHAGEDHGMPPPYRSNPQKFA
                     YIADHMQGGIMVAAHLGSHNMWDDVERYLVGKNIYLDTSMGFEYYGKEQFLRIVKAHG
                     ADRILFASDSPWSNAKNEIDAINSLPINQTSKDLILGGNAARILGLS"
     gene            37053..39188
                     /locus_tag="PLF14_00145"
     CDS             37053..39188
                     /locus_tag="PLF14_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT69742.1"
                     /translation="MKKNKQTKIDFGYAFSEPHRICVCLPNSSNKTLYDCTKDSLTML
                     WSDSDTTHNPLGAYKRVRLDWKVKIIAKCNDVAMEAFSWERIEGYIPSLLYKAKAEGA
                     NLTVQCVATRLGDVIKMSVTNTTGSDKNIFIDACVLDNTINTKWADFDCEYSVVNPIF
                     NDASDRIVIMDASLTDTVPSRRESVTYAYTLKAKETRESMLIRPNKKYNDDIEVMLKT
                     NWNALYEQGLNEWRNIIKKAPVFDLPDPMIENAYKACLCDIFVMREEMADGRMAGLAG
                     TEMYRSSNTGEPCFQALTLGKYGFFEPAKENIEFVASFQLNNGSWEDDKQWGKYMWAS
                     SGWKSRCIQHYYLRTKDRAFLEENFERMLASARWSKAQREKTKKKNSPDSPEWGLMPR
                     GMGDGGLKNGDDLFGVFYPHNFMHCMGLEAAVWAAKELQREDVYDELLATYLDHKECI
                     LKSLQIGHIKEPDGSVWIGSCAHNTSGSRWAVADAVYPTNILPVDHPLAVGTMNKLQG
                     NISEGGLPKNLGWIHDGLWVAIALDAMAYVNILADKADISADYLVAALNHGTPLFTWC
                     EERTEEKGAKRITGDLQHAWTPICVAQFIRDMLVSDYLFKDDMLHLTCAVPRYFYKEG
                     NAMNIKEAPAFQGKISFSIEVSKNTVTFTADLCSFDINRELCLHLRLPNRNLEVTVQS
                     ISGGSAVITEQKAVIKPDTNKIKAVFNIG"
     gene            complement(39223..40347)
                     /locus_tag="PLF14_00150"
     CDS             complement(39223..40347)
                     /locus_tag="PLF14_00150"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT69743.1"
                     /translation="MSLTSKERLMRIFANKPIDRPAIKLWGSWNYRKGDKLLHPSYEP
                     AMIKAAEKTDLVVNASTPFFMFCGQVDIVRRKNVEINDPLWYEEHIVFHTPMGQLRRV
                     DRISKIGEPGFTIEYPVKQPEDIKRMLSMPYEPFPYIDNIEEQQQLLGDRGIVLYRID
                     HIAYALYRMIGSENLAYFTHDCRDLLMEALDVFSKRLYAHVEEALNHGVRVPFSWVGP
                     ELLIPPLLSEKDFMDFCFDYDKHICDMVHNAGSYIWVHCHGKVRNLIENFIEMGVDVL
                     NPLEPPKNGDVDLDEVARTFKNRIGLEGNIEMQDFYDESEEVIREKIKTCVQSGKQSN
                     RFIMCTSAGFMDFPTPSKQYLDNIMTYIDYGYECVNNARY"
     gene            40534..40806
                     /locus_tag="PLF14_00155"
     CDS             40534..40806
                     /locus_tag="PLF14_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOT69744.1"
                     /translation="MPRFDGTGPRGMGPLTGRAMGYCTGARRPVGRYYGRGGGRGRGF
                     GVCNRLYYYDDAPLYDEKPSKELLLNQKKFLEEELASINKQIYEYE"
     gene            complement(40938..42401)
                     /locus_tag="PLF14_00160"
     CDS             complement(40938..42401)
                     /locus_tag="PLF14_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009144574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HOT69745.1"
                     /translation="MGRDLRKLTLLHSNDMHGDFMSENIDDKLVGGVAMLSGYIDKVR
                     QEEENVVYVIAGDMFRGSIIDQEYRGLSTIEIVNMLSPDVVTLGNHEVDYGVAHLLFI
                     EKLAKFPIINSNLYIKTNHTRLFRPYLIKEVGGIKILYIGILTEEVIAQTKQETLIGS
                     IVDVYEAAKEVGRICNAYSQIDIDFTILLTHIGHEEDKKLASMLDPAWGVDIIIGGHS
                     HTFLEQPDIVNGIVIAQAGTGTDQIGRFDIIVDKDNNCIEDFTWQAVPIDETHCPVDK
                     DLEEIILQYHEKTNEKFSRIITRFKRALTHPKRSQETELGNLCADAVVDNLGLDIMLF
                     ASGSIRKKELGPILTLKDFKECFPFDDKIYRIKLTKKQIEKAFNTILGRDLSDHVEFY
                     QVSRGFKVKYSKKENRVTELSLESQPLDDNHIYSVGLQEYHFNNMADFIGITKEEAEA
                     NSPIKVVTTSSGDVLEEAFLGMVLADPKVEGRIVIEY"
     gene            complement(42502..43800)
                     /locus_tag="PLF14_00165"
     CDS             complement(42502..43800)
                     /locus_tag="PLF14_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT69746.1"
                     /translation="MYRTSLEYLYKWKEKDNRKPLIIRGARQVGKTWLMKEFANKAYK
                     SHAYINFDNNMQMKQLFLQDLNVDRLIMGLELYTGKKIVPSETLIIFDEVQEVPNALT
                     SLKYFNEDAPSYHIVCAGSLLGIALHPGTSFPVGKVEFMDLYPLSFSEFMLAMGKEQY
                     AELLESKDYEMITTFKQEYINLLKQYFYVGGMPEVVAHYAVNKDFQGVREIQRRILDA
                     YEQDFSKHAPNEIVSRIRMLWNSIPAQLAKENKKFIYGLIKEGARAKEYETALLWLTD
                     CGLVHQVNRVNAPNIPLKAYEDLKAFKLFTLDVGLLSCMVGLHQSALLSGNDLFREFK
                     GALTEQYVLQQLKTLDNLNVYYWTNDRNRAEIDFLIGSEGRVIPVEVKAEVNLQSKSL
                     KTFRDKYDIEISVRTSMSDYKKEERLINLPLYAISNIKKI"
     gene            complement(44012..44851)
                     /locus_tag="PLF14_00170"
     CDS             complement(44012..44851)
                     /locus_tag="PLF14_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOT69747.1"
                     /translation="MIKTARQLKDLIRNVAKGDSGKSQLLFRNYAMERFLERIALSKY
                     RNNFILKGGMLVSSIVGLDMRATMDMDATILNLPLNPEHARIIVEDITAIEIGDNMAF
                     SVKDISNIMDEADYICVRLSLEALLENMRIPFRIDISTGDSIIPKEITYSYKLMFEHR
                     YIPIRAYSLETILAEKIETVLIRTIFNTRLRDFYDIYILQNTRMDIDINILTVALTAT
                     CRNRNSEKVLTDYRYILKEIENNPTMREFWISYQRKNSYAADITWETVIKSIRALCDS
                     CIL"
     gene            complement(44851..45441)
                     /locus_tag="PLF14_00175"
     CDS             complement(44851..45441)
                     /locus_tag="PLF14_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive phage infection protein"
                     /protein_id="HOT69748.1"
                     /translation="MKYDYIDKILADNDGILKTADIVAAGICKETFYNYAKDTELEKA
                     AHGIYISPDVPVDEMFLLQTQIPKVIYSHETALYLHNLSEKEPIPLTVTVPAKYNNPT
                     LTEKGVKIVYVKKEWYSLGICQMSSPAGYDITAYDMERTICDVIRKRSYMDISAFNYA
                     LTEYMKQKGKDLNRLMEYAKIMRLEKRIRETMGVLF"
     gene            complement(45575..45650)
                     /locus_tag="PLF14_00180"
     tRNA            complement(45575..45650)
                     /locus_tag="PLF14_00180"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45615..45617),aa:Ala,seq:ggc)
     gene            complement(45698..46675)
                     /gene="tsaD"
                     /locus_tag="PLF14_00185"
     CDS             complement(45698..46675)
                     /gene="tsaD"
                     /locus_tag="PLF14_00185"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOT69749.1"
                     /translation="MIILGIETSCDETAASVVKDGRIILSNIISSSAKIFEEYGGVVP
                     EIASRKHVEYILPVIDRAIKEAGIKREDIDAIAVTYGPGLIGALLVGVTAAKALAASL
                     NIPLIPVNHLKGHIAANYLTHKELKPPFVCLLISGGHTNILDIQGYDDIKILGGTRDD
                     AVGEVYDKVGRVMGLVYPGGPKIDKLAKEGNPEAISFPRTKFRDNVFDFSFSGLKTAV
                     INYIHSKEQKNEDYSKEDVAASFQKAVTDVLVDNVFETKSKKIVVAGGVAANKGLREA
                     LNKRAAKENTEVFYPDIHLCTDNAAMIASAAYYINKEDFDINADASPDM"
     gene            complement(46672..47457)
                     /locus_tag="PLF14_00190"
     CDS             complement(46672..47457)
                     /locus_tag="PLF14_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOT69750.1"
                     /translation="MTIWQAILLGIIQGLTEFLPVSSSGHLVLFEKILGIETPTLTFN
                     IAVHFATLVAVCVVFWKDIIELVKKPVQKLTLWLIIATIPAALAGVLFNDFFEGLNDS
                     GATLGIGFLITAVLLIVSSVLKPGTVEKENMKWYDALIIGVIQMVAIVPGISRSGSTL
                     TGGLFRKLKKEDAVKFSFLLSIPVILGGFILEIYGAIKVGLGNIEVLPIIVGMIFAGV
                     SGFFAIKLFVKTVLKGKILYFAYYVAALGIFCILDQFIFHIVL"
     gene            complement(47454..48725)
                     /gene="clpX"
                     /locus_tag="PLF14_00195"
     CDS             complement(47454..48725)
                     /gene="clpX"
                     /locus_tag="PLF14_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOT69751.1"
                     /translation="MSDEIKKIRCSFCGKSEDRVQRLISGPGVYICNECVDLCSDLLD
                     TELGYDDEEEEVTETVIDEVPHPEEIKRQLDTYLIGQDDAKKAISVAVYNHYKRISYN
                     VAHEPDDVEIQKSNILMLGPTGTGKTYMAQTLAKILNVPIAISDATTLTEAGYVGDDV
                     ENILLRLIQAADYNLQRAQRGIVYIDEVDKIARRGDNPSITRDVSGEGVQQALLKILE
                     GTVANVPPQGGRKHPTQEYIQIDTTNILFICGGAFQGIDEIILNRLGKRAIGFGAEIH
                     KTNSVEKDRILKHIETQDLLKYGLIPEFVGRLPVIVTLEQLTEDILVSILKEPKNALV
                     KQYKKILEMDGCELVFEEKALRAIAKKTLERKSGARGLRSIIESALMEVMYSVPSDPS
                     IVKCIVEESTILNNTPPKLVYKGNLLEENVS"
     gene            complement(48735..49316)
                     /gene="clpP"
                     /locus_tag="PLF14_00200"
     CDS             complement(48735..49316)
                     /gene="clpP"
                     /locus_tag="PLF14_00200"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOT69752.1"
                     /translation="MNTIPYVIEQTSRGERSYDIFSRLLKDRIVFLGSEVNDIAASLI
                     IAQMLFLEHEDPEKDIKLYINSPGGSVTSGLAIYDTMQLVKCDVSTMCVGLAASMGAF
                     LLAAGTKGKRYALPNSQIMIHQPSGGIQGQATDIRLHTDWLLKVKDNLNRILAEKTGQ
                     PLEQIAKDVERDYFMSSADAKQYGIIDEVLNKR"
     gene            complement(49340..50755)
                     /gene="tig"
                     /locus_tag="PLF14_00205"
     CDS             complement(49340..50755)
                     /gene="tig"
                     /locus_tag="PLF14_00205"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOT69753.1"
                     /translation="MESKIVKKEGYKVEMEIALTKEEFDGYITKAYNKDKNKYTIQGF
                     RKGKAPQYLIERIYGKGIFYDTAVDMALNAEYPKAIDNLKLDVVDYPHVDLKDMDEEN
                     GITFSINVDVKPDVELGKYKGVEIKKAEVEVSDKDVEDALNREADKNSRMIGIEDRPV
                     QDGDTVIIDYEGFVDNVAFEGGTAEKQQLVIGSKTFIPGFEEQIIGHNINDEFDVNVK
                     FPDEYHAEELKGKQAVFKVVLHEIKVKELPVIDDEFIKDISEYDTVEQYKEETKKKML
                     EERQKRAEDSMRSAALATACKNAKVDIPNAMIERATDNIIDNMRMEMQYSGYTLEQYL
                     EYTGMKMEDIRSQYKEQAGMMVVRDLVVEAIKNVENIQVTDEEIEELLNKDAETYKME
                     PDRIKQAIGDYKQYYTDKIASDKVVDLIYSSAKKVKEVKEDKKDKEVKEKKSVDKKKK
                     QDEEPEKKAAPKKTAEDKSEK"
     gene            complement(50994..53600)
                     /locus_tag="PLF14_00210"
     CDS             complement(50994..53600)
                     /locus_tag="PLF14_00210"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOT69754.1"
                     /translation="MSITIKDLTVNYLTNPLGIDEIPRFSWKLAQDGRGNKQRSYRIK
                     VMTGSKVVWDSRNVRSNESVLIEYKGKKLLPRTTYRWNVSVTDKKGERAQSEDSFFDT
                     GKLEEEWVGKYIQAGYRVPRELAASSPYLRKRFDITEDFSKAMLYITGAGFFEPYING
                     KKINDHIMDPPFTAYDRTLLYYTFNVTEYLRKGSNMLGVILGNGFFNYPTIDQWNTHA
                     ASWKGSPKMICELYITTKNNKQICIPSDITWESHMSPITFNSLRNGEFYDARLEIPAW
                     CEPDALVDDWVNVSISRDPGGLLKANEMPSVKVDREYPAISVKNVAKNTYLFDFGQNM
                     AGFVRIKVTGPKGTEFVIKMNEKLKDDGTLDTVYNSGFTKTGEFQTDKYIKKGDGEET
                     WQPRFVYHGFRYAQIEGFTSQPDLSSATAIFLHTAVKEIGQFSCSDDIINQIQKNTRM
                     ATYSNMYGLLTDSPHREKNAWTGDTNVSTEQILFNYMSGPLWMKWLNDIKDSMKAKGN
                     IPCMVPTGGWGYNWGNGPDYSSVLTWLPDSMYTYYADKYVIRKYYQYMKRNMKYMLSM
                     MQNDVCVNDYGVGDWCAPFEGPAISVNMSSFKAPIDLTDTACMYTMANVLEKFAKLLG
                     HRDDAKEFNKTAKKIKAGFRKTFYDPQTGRLKGDGQTCYAAMLYHNLYEGNKEYDLIY
                     SHLIRTINEADGHLDYGILGNKYVNNILGRQGRADLIYSMITKDTFPSYAHEIKELGA
                     TTLYETWNGEGSRNHHMFGDISATFFKYFAGIRPDPDYPGFKKFTIAPAFNTPLVNVD
                     AVFESMYGTIRSSYVKEGKVISMEIEIPTNTSAVLQTGAGMEIYEDNNLLGKEYLELE
                     SGKYKLEIRKTV"
     gene            complement(53791..54996)
                     /gene="tuf"
                     /locus_tag="PLF14_00215"
     CDS             complement(53791..54996)
                     /gene="tuf"
                     /locus_tag="PLF14_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOT69755.1"
                     /translation="MAKAKFERTKPHCNIGTIGHVDHGKTTLTAAITKTLALSDKNIV
                     YKAYEQIDAAPEEKARGITIATAHVEYETKNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVVSAADGPMPQTKEHILLARQVGVPYIVVYMNKTDQVDDPEILELVEMELREILN
                     EYEFPGDDIPIVMGSALQALQSTSTDPNAPEYASILKLMETVDSYIPTPQRDVDKPFL
                     MPVEDVFSITGRGTVATGRVERGTVKIQDTVEIVGLTEKIASTVVTGVEMFRKILDFA
                     QAGDNIGCLLRGVQRTDIERGQVLAKPGSIKPHTDFVGQVYVLTAEEGGRHTPFFTGY
                     RPQFYFRTTDVTGVANLPEGVEMVMPGDHIDMRIALITPIAMDEGLRFAIREGGKTVG
                     AGSVVKIMK"
     gene            complement(55074..57149)
                     /gene="fusA"
                     /locus_tag="PLF14_00220"
     CDS             complement(55074..57149)
                     /gene="fusA"
                     /locus_tag="PLF14_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOT69756.1"
                     /translation="MPRDYALEFTRNIGIMAHIDAGKTTTTERILYYTGKLHRMGEVH
                     EGAATMDWMEQEQERGITITSAATTAYWKDYRLNIIDTPGHVDFTAEVERSLRVLDGA
                     VSVFCAKGGVEPQSETVWRQADRYKVPRIAYINKMDTTGADFFRCVEMMRKRLNANPV
                     PIQLPIGKESDFTGIIDLVKFKAYFYRDDLGNSIEELDIPDDMLDKATEYRIKLIESV
                     CDESECLMEKYLEGEDVEESEIKAALRSGTVNLTITPVLCGSSYKNKGVQKLLDAITD
                     YLPSPVDVSDIEGVSMHDHSKVITKPSRDDEPFAALAFKIMSDPFVGKICFFRVYSGT
                     LETGSYVLNTTKDKKERIGRILMMHSNSRTDISKVYSGDIACAVGLKFTVTGDTLCDP
                     KDPVILESMQFPEPVISIAVEGKDKINQDRMALALQKLSEEDPTFKFKTDKDTGQVLI
                     SGMGELHLDIIVDRMRREFSVEATKGKPQVTYKETITKPARAEGKFIRQTGGHGQYGH
                     VVLEVAPSKPGEGFTFIDKTVGGVIPKIYIPAVEKGIRESLSNGIQAGYPIVDVTVTL
                     LDGSYHEVDSSDMAFMIAGSMGFREACMKADPILLEPVMRVDIFVPENCLGDVLGDIT
                     AKRGKIENVETGKTESVITAIVPLKEMFGYATDLRSNTQGRGTFTMQYDHFERIPDDL
                     AAKILKTNY"
     gene            complement(57165..57635)
                     /gene="rpsG"
                     /locus_tag="PLF14_00225"
     CDS             complement(57165..57635)
                     /gene="rpsG"
                     /locus_tag="PLF14_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOT69757.1"
                     /translation="MPRKGHVPKREILPDPVYKDKVVAKLINNIMLDGKKGVAQRICY
                     DAFDIIKTKTGKDPLEVFKQALENIMPALEVKARRVGGATYQVPVEVRPERRQALALR
                     WLVQFARNRNEHTMKQRLAAEIMDSVNGVSNTFKRKEEMHRMAEANKAFAHYRW"
     gene            complement(57748..58158)
                     /gene="rpsL"
                     /locus_tag="PLF14_00230"
     CDS             complement(57748..58158)
                     /gene="rpsL"
                     /locus_tag="PLF14_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOT69758.1"
                     /translation="MPTFNQLVTKGRQVSKKKSLSPALQKSMNTIRKRTTSVACPQKR
                     GVCTVVKTNTPKKPNSALRKIARVRLSNGYEVTAYIPGIGHNLQEHSVVLIRGGRVKD
                     LPGVRYHIIRGTLDTQGVANRKQGRSKYGAKKGK"
     gene            complement(58191..58433)
                     /locus_tag="PLF14_00235"
     CDS             complement(58191..58433)
                     /locus_tag="PLF14_00235"
                     /inference="COORDINATES: protein motif:HMM:NF013417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HOT69759.1"
                     /translation="MIKAASQDKKVIGLKQSAKAVLEDRAMTVYIARDADRSLIEPLA
                     ELCEKKGIEIRYADTAKQLGLLNGISVKASAVCILK"
     gene            complement(58433..62131)
                     /gene="rpoC"
                     /locus_tag="PLF14_00240"
     CDS             complement(58433..62131)
                     /gene="rpoC"
                     /locus_tag="PLF14_00240"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOT69760.1"
                     /translation="MQDYTNFDMIKIGLASSEMIKEWSRGEVKKPETINYRTLKPEAG
                     GLFCEKIFGPTKDWECHCGKYKRVRHKGIICDRCGVEITKSKVRRERMGHIELAAPVS
                     HIWYFRGIPSRMGLVLDMSPRALERILYFNSYVVLEVNNEDVKVNDKTIEVKDLLTEL
                     QYRELIDKYGRDSFKARMGAEAIQILLARIDVEEESALLKEELQTSTGQKRGRIIKRL
                     EAMESFRVSGNKPEWMILDTIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRL
                     KRLLELTAPDIIVRNEKRMLQEAVDALINNGRKGKPVVGPGNRPLKSLSDMLRGKQGR
                     FRQNLLGKRVDYSGRSVIVVGPELMMYQCGLPKEMALELFKPFVMKRLVTDKFAQNIK
                     SAKKMVERVRNEVWDILEDVIKDHPVLLNRAPTLHRLGIQAFEPVLVEGRAIKLHPLV
                     CTAYNADFDGDQMAVHVPLSAEAQSEARILMLSANNLLAPKDGKPITVPTQDMVLGGY
                     YLTTAKDGEIGEGRVFRNFDEMLMALHEKTITLHSKVKMRTEREVDGKVVSKIIDVTC
                     GKVIFNEAIPQDLGFVDRTNPDTMFNLEIDQMVGKKNLSEIIERCITKHGIAKTAEVL
                     DKIKSIGFRYSTQGAISISVSDMIIPSSKNEILEATEKKVDKITHDFRRGLITDEERH
                     DQIIKLWRKTTDDVTKMLMEGLDAFNPLNVMSRSGARGDMNQIKQLAGMRGLMFDTAG
                     KTIEIPIKSNFREGLNVLEFFISSNGARKGLADTALRTADSGYLTRRLVDVSQDVIVR
                     EADCGTEDGIYVSDIVDSGEVVVKLKDRIYGRYVTDPVVDKKTGEIIVEAGGYIDADA
                     TKRIVDAGIKEVKVRSVLTCKSELGVCAKCYGMNLASSKPVNIGEAVGIIAAQSIGEP
                     GTQLTMRTFHTGGIAGDDITQGLPRVEELFEARKPKGLAIISENDGIVKINESRKRRE
                     VIVTGEDESHTYTIPYGSRLTVVDDQEIKKGDKITEGSENPHDILRIKGVEGVHRYIA
                     CEVQKVYKLQGIDINDKHIEIIVKQMLRKVKVEDCGDTNLIPGSYCDATDFYKQNKLA
                     EQEGKQPATGEVVLLGISKASLATDSFLSAASFQETTRVLTEASIKGKTDNLIGLKEN
                     VIIGKLIPAGTGMNAYQDPYLEMDPIDDEEDFIAEEGYEDEVDLFDEMNDDEETLDQD
                     DSEEYDDEVLSEDADSDDISESEEEQTE"
     gene            complement(62135..65884)
                     /gene="rpoB"
                     /locus_tag="PLF14_00245"
     CDS             complement(62135..65884)
                     /gene="rpoB"
                     /locus_tag="PLF14_00245"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003360188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOT69761.1"
                     /translation="MIHPIQYGKRERMSFSKIKEVLEMPNLIEVQKKSYQWFLDEGLR
                     EVFKDVSPVRDHTDNLMLDFIDYRIADKPKLDVQECKERDATYQAPLRVKVRLIRKDT
                     GEVKEQEIFMGDFPIMTDTGTFIINGAERVIVSQLVRSPGAYYAEKKDKAGKSLFSST
                     LIPNRGAWIEYETDSNGVLYVRIDRTRKLPLTVFLRALGFAKDSDITAVFGEDYKLDA
                     TYAKDETKTREGGLFEIYKRLRPGEPPTIESAENLFEGLFFDARRYDLARFGRYKFNK
                     KLSVAPRIAGTVSAERIIDAETGEILVEADEVIDEAKAIAVENAGINVVKVKCDNHVH
                     VVIGNETVDASHFVDFNHTALGLTDKVNYKVLKEILAECSTNEEIKEAFEKRRYDLCP
                     KNITRIDIIASISYLLGLDYGLYHTDDIDHLGNRRIRSVGELLQNQFRIGFARMERVI
                     REKMTIQDIDIVTPQALINIRPVTAVIKEFFGSSQLSQFMDQPNPLAEITHKRRLSAL
                     GPGGLSRERANFEVRDVHYSHYGRMCPIETPEGPNIGLIASLSTYARINEYGFIETPY
                     RRVNKETHTVTEEIDYLTADEEDIYIVAQANSELTPDGRFATPRVSCRYRNNKVMRDS
                     KDVDYMDVSPKQLVSVATSMIPFLENDDANRALMGSNMQRQAVPLIRTEAPIIATGME
                     YKVATDSGVCVLAKRAGEVKNVSADRVVIDTVDGERDEYKILKYKRSNQGTCINQKVI
                     VKKGDKVEAGDVIADGPSTDGGEISLGKNVLVGFMTWEGYNYEDAILISERLVEDDVF
                     TSIHIEEYDLEARDTKLGPEDITRDIPNIGDDALKDLDERGIIRIGAEVQAGDILVGK
                     VTPKGETELTPEERLLRAIFGEKSRDVRDTSLRVPHGESGIVVDVKLFTKEAGDDLNP
                     GVNQVVRVFIAQKRKIAVGDKMAGRHGNKGVISRVLPEADMPFLEDGTPLQIVLNPLG
                     VPSRMNIGQVLEVHLGYAAKALGWKIATPVFDGASEMDIIECLKMANLSEDGKTVLYD
                     GRTGEPFDNRVTVGYMYMLKLHHLVDDKIHARSTGPYSLVTQQPLGGKAQFGGQRFGE
                     MEVWALEAYGAAYTLQEILTVKSDDVVGRVKTYEAIVKGESIPKSGIPESFKVLVKEL
                     QSLALDVKILKSDHSEVMLRDGSEDDIPRLDINISGTEDYTLDNDELFNMELAKVDDD
                     SEDQDNDSEEMPGMSMKTADDDDTGVDYSKISDVQDIVDLDDMGE"
     gene            complement(66144..66440)
                     /locus_tag="PLF14_00250"
     CDS             complement(66144..66440)
                     /locus_tag="PLF14_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT69762.1"
                     /translation="MKKTDYDDMTGFEEGILEELALDERDVALLVLIEKEIESTLKNI
                     RIFDLQTNKRMEEILSEEIYNQGEKINMLMDHASPGLKKYLEEYMTKIKETYLN"
     gene            complement(66427..67104)
                     /locus_tag="PLF14_00255"
     CDS             complement(66427..67104)
                     /locus_tag="PLF14_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT69763.1"
                     /translation="MTRRITALILVVIMVFTLTACNNKKTPDEGTPSYVVTTEQNWPS
                     EYLGADILPKIKGGEVNYYKFYGQILRIYLYNVDDESFMKYIETLKGVGYKNISVETK
                     EAFAGSLDDYYLAMNRDVDTHKMVIELTTPLTWPKEGYITMLSAPPGAKVLQIIGESN
                     NITVVTEDIGKEKFESYTADAIAAGFTAILVTSETVFVAENNDYILTIVRQPDKNTMD
                     IGIYEKN"
     gene            complement(67116..69263)
                     /locus_tag="PLF14_00260"
     CDS             complement(67116..69263)
                     /locus_tag="PLF14_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HOT69764.1"
                     /translation="MFITEAVVLKEDKVLKAANLSEEQVQRAREGTIAHSILKSHNTS
                     ASNHMLKIKFDAVASHDITYVSILQTASACNLEKFPIPYVLTNCHNSLCAVGGTINED
                     DHLFGLSAAKKYGGIYVPAHLAVIHQYMREKMAGCGKMILGSDSHTRYGALGTLAMGE
                     GGGELVKQLLGKTYDLAYPKVCLVYLTGTLNQGVGPHDVAIYMIGKVFKDASVTNKIL
                     EFAGPGVNNLSMDDRIGIDVMTTETACLSSIWVTDDKTKEYYEIHGRKEDYKKLSPAK
                     EALYDCALMIDLSKIQPMIALPYHPSNAYTVKDFLENSEDILEQLNLGSLKVYADSKK
                     RRFFTGQGIIAGCAGGSFENICEAAAVLQKGDTGSGSFSLSVYPQSQPVAYALNKAGI
                     TNKLMESGVVIKTAFCGPCFGAGDIPANNVLSVRHTTRNFENREGSIPKDGQAAYVAL
                     MDARSIAATALNKGYITAGTDIEYVKDIPVYEFNGNIYEKRVYNGYGKPDKNQELIYG
                     PNIKVWPEIPRLPKNLLLKVAAVLNDEVTTTDELIPSGETSSYRSNPMALAEYTLSRR
                     APDYVNKTKLAVREAGVDGHLIFAKKPGDGSAREQAASCQRVLKGYANIAYRYATKRY
                     RSNLINWGLIPFTVKQDPALTVDEYVYIPDIRDAVLSGKEQVIAYVSATKEDALQRTD
                     KARAVRLYMEGLSDDERDVLAEGCMINYYRKSM"
     gene            69343..69966
                     /locus_tag="PLF14_00265"
     CDS             69343..69966
                     /locus_tag="PLF14_00265"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOT69765.1"
                     /translation="MKYKHIIWDFDGTLFDTYGEIAKVIEEVLKNRGIVENLDWIVKN
                     LHISLTHSLTHLSNKHTLDYNEFKDTFMQAEERMNVKEALPFPGVKEIIDSTEGYTFM
                     VTNRAESVFRFLEYRDYRKYFKEILHRNSGFPLKPGSEAVEYLIEKYGLDRKEMLFIG
                     DRDVDIECAINAQIHSCYYDSHNIPNIAPCTYYTKDYFKLLDILKTA"
     gene            complement(69940..70899)
                     /locus_tag="PLF14_00270"
     CDS             complement(69940..70899)
                     /locus_tag="PLF14_00270"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HOT69766.1"
                     /translation="MENLRSRKLQPYQSFLLITAVMIIFFTVQTVGIFFLGDNLYLYQ
                     TACEVLGLAVPVLLYMLFTGRFKDNVHFKKVSGKNILIILAVVTLFIPVSMLLATFNT
                     WIVDNLFGTTPGLEDIVLMENFGDYLKSILVIALVPAICEEIAFRTAFMSGLERLGKV
                     GCFFFCSLFFSLAHSIPEKLLSTFALSVLLCYFLYRTGSLLAAMMGHFINNFIALTIT
                     YATRNIPPESGGIEFLEAYGLSEYGVSFIVLGAISLLVLPVIVILLRRFRKNTESTKI
                     NIIKETKLDSNGKVAFLVAFTIYIIPVAIIIVTNALSGFQNVK"
     gene            complement(70899..71867)
                     /locus_tag="PLF14_00275"
     CDS             complement(70899..71867)
                     /locus_tag="PLF14_00275"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOT69767.1"
                     /translation="MIKIDLDNGIHSLSMDKLAFGTGVFSDYADKESYFLLMDKFYEH
                     FKTYDTARCYNEWLVEGKDISEYMLGEWMKSRKNREDLIVITKGGFPKNLKLKKDVME
                     RITKEELQYDIDKSLSVMNIDYVDIFLLHRDDLSKPVEYIIPILQDFIKQGKTRFIGV
                     SNWTSKRIEEAQAFIASENLVPLSVSQCYFSLAESTPERFDDETIVCIDDEQYDWYEK
                     KKLPLMAYTSQAKGFFSKYNTVQLKDGIVKKFVSDINIERAKRAKMLADEYSVSPTCI
                     ALAYLTNNPVQTCAVIGSKNLSQLEDSIGVKDILLSQAQIDWLKGE"
     gene            complement(71867..73045)
                     /locus_tag="PLF14_00280"
     CDS             complement(71867..73045)
                     /locus_tag="PLF14_00280"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007933729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOT69768.1"
                     /translation="MFNEKITQSLSKSSFIRAMFEEGNRLKAIYGEENVFDFSIGNPE
                     MEPPAKVKECLKKIACSEEKGMHKYMSNAGYDATRKAVSESIKKDYGYDVPFSNIIMS
                     VGAAAGLFVTLKSVLNPGDEVVVFKPYFAEYGFYVMHAQGVLVEADCDEFFLPDIDSF
                     KSVITAKTKAVIINTPNNPSGAVYPKEVLLALNDAIRQAERKYNSDIFVISDEPYVKI
                     VYDDITVPCVFDCFDKAIIVNSYSKSLALPGERIGYIAIDPKIPDYQLLVNICIFNTR
                     TLGYVNAPAIMQKVIQYAYDAKVDIDDYRERRDILYNHMVKCGFSVKKPQGAFYLFVK
                     SPVEDEMEFVEKAKEYNILVVPGRGFGMKGYVRMCFCISKKTIQASLPKITALAREYF
                     "
     gene            complement(73120..74895)
                     /locus_tag="PLF14_00285"
     CDS             complement(73120..74895)
                     /locus_tag="PLF14_00285"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HOT69769.1"
                     /translation="MPLDGIAVKCLANELNGICKNGKIQNIYMPEKDEVILGIYANGT
                     SYNVALSLQPSSPGIYFKEEKINNRKTPYGFCMFLRKHIGGGFISEVYAKDYERIIYF
                     DINCTDEFSGNSVKTVIMELTGRNCNLIVVNSSCKILDALRHVDESMSSVRRVMPACE
                     YVLPPIQDKIPIEDVQWEHLLKYADFPLSDAIFRTVLGMAQVFAKEICVRAGVDETKT
                     VKNISKEEAIQVMSVIKKTAQDISANTYSPIAVYSNEKKTITDCYCFNLSLYKNISEF
                     TVKKYPLYIDALSDHLNLKNKRAVFIQASDVVRKSVSAHLVKAEKKLLIYNNNLSDRE
                     KIDKYKLYGELLTANIHAIKQKTDYVELLDYYTDRIVKIPLDKSEDALKNAQSYYKKY
                     KKGIAAYNYAVNAVKGIKKEIEYLKSVRASLELCENLSDIEDIKKELVLEGYMKEKLK
                     IKKGRKASQPEAVSFNPVKYRSKEGYEIYVGRNNTENDKLTFDYAQNFDIWLHVKNLH
                     GSHVIIKNPAKCEEFLPDKTLTEAAIIAAYHSEARTSGQVAVDYTFIKNVKKPKGAKP
                     GYVNYYNYYSAYVKADELVAKKLRE"
     gene            74989..77241
                     /locus_tag="PLF14_00290"
     CDS             74989..77241
                     /locus_tag="PLF14_00290"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOT69770.1"
                     /translation="MELLSPAGNMQCLKAAVQNGADAVYLGIKTFSARANADNFTFEE
                     LTEAVRYCHLRDVSVYLTLNTLIDDENMQTALNNAKKAYSSGIDGIIIQDLGLAAQIR
                     KMLPDLRLHASTQMSVIHPKEALLLKSLGFKRIIAAREATLEQIKALVATGIEVEVFG
                     HGSLCVCYSGQCSLSYFNGGKSGNKGGCAQPCRLPYSLFIDNRNISCKNYLSTKDIAV
                     MDDIDRLVSAGVVSLKIEGRMKSPEYVAIITKTYRKALSGTLTDKDRQDAMMAFNREG
                     FSKGYMFSESGKDMMAYAFSGNTGIRIGTVKKINKEKNLIQIESNKELINGDGISFPD
                     SSIGTYVNILRKNGNLYGLICRNKLPEPNEPVYLTYDKQLTEQAQKSYADDFLRKVPV
                     RGHFISKAGQRASFTVTDNSGNTYTSCSDIDSQAAKNPNADDDILKNLNKTGNTVFEF
                     SSLTKDTDTATFLPAKTVNALRRDALDGLAELRQSIKNRNSKTELFPKYENLSLSSES
                     FNTEKTEKNEKRDTPVNRDISNKKTGISLFFFEDRLDFDIADIDADRIYVPSTMYKRY
                     LRDDRVYLYIPIGANPQDTAGNRQRIISSSMGILDIPADKVLDAGINCLNTYTVDALS
                     GYQGVKAIHLSYELPVEDIRRFANFNGNISLEYLVYGRVAVMKTKYRIKDIFTDTDTQ
                     EIYLLDRHKKRIDIVTYPDCDTTYLLGAFTVNNLLHKDLIRASGADTLRINVYKETAD
                     EIKDIIKRAE"
     gene            complement(77242..77889)
                     /gene="rpe"
                     /locus_tag="PLF14_00295"
     CDS             complement(77242..77889)
                     /gene="rpe"
                     /locus_tag="PLF14_00295"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016286171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOT69771.1"
                     /translation="MIKISPSLLACNFARLEEEVRKIEEGGAHYVHVDVMDGHFVPNI
                     SVGIPIVQALKSITKLSLDVHLMISDPDRYIEEFAKAGADIITVQQEACIHLDRTVRY
                     IKEQGKKAAVALNPATDIRVLEYVLPMLDMVLIMSVNPGFGGQKYIPYSTEKIKALKK
                     MIDKMGLKTDIQVDGGVTVDNCREIKAAGANVLVAGSAIFQSKDIKETIRKLIEY"
     gene            complement(77889..78776)
                     /gene="rsgA"
                     /locus_tag="PLF14_00300"
     CDS             complement(77889..78776)
                     /gene="rsgA"
                     /locus_tag="PLF14_00300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOT69772.1"
                     /translation="METGLITKALSNRYTVRTDKDEYICKPRGLFRKEGFKPLCGDLV
                     RFDITDYADKEGYIISIEERKNSLVRPPVANVDVAVIVFATEYPTLDELLIDLLILAF
                     RTNDIEPILCLNKCDLSAKEDIDDVLRQYSGSGCKIVITDAKHKTGLDPLKSMLSGKV
                     SVFAGQSGAGKSTLINAITGTADMEIGILSEKIGRGKNTTRHCELLSFEGGYIVDTPG
                     FSKFDPGLLQPDLIKGMYNEFLPYIGQCRYNDCKHINDDHCAVLQAVEKGEIPIERHL
                     RYEMIYNMSEEALRVRRGY"
     gene            complement(78777..80873)
                     /gene="pknB"
                     /locus_tag="PLF14_00305"
     CDS             complement(78777..80873)
                     /gene="pknB"
                     /locus_tag="PLF14_00305"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HOT69773.1"
                     /translation="MNPVILNNRYELLSKMGIGGMAYVYKAKDKALNRIVAIKTLKDE
                     YAQDKDFIKRFKDEALSVAKLSHESIVNVYDVGYDNNMNYIVMECVEGTTLSNYLESA
                     GPLDIKEVVDISMQIFAALEHAHNNGIIHRDIKPQNILISKNGRIKVTDFGIAKAINA
                     QTLTTDNKTFGSVHYISPEQAKGGFLSSSTDIYSTGIVMYEMATGHVPYDADTPISIV
                     MKHLQDTPIPPIDYRRDMPEELNKIILKAIQKKPSDRYQTAADVMADLKKLNNNANSK
                     GDITLDFINGEVLPSDETVTLPEVTEDDLFLEEDNAIVRGNTGKVIKIKEKTDKITGP
                     RLINYDEEYNVPLIYARGRKKKEKKYFFTTLIALICVLSLSAFFIVFAIDEMAKVVVP
                     EPKEYEVLNYVTYEYETIKKKLETDFNISVLMKEAYSDETPPGIIMDQDVEPGRIFKE
                     GAKNTITFTVSVGSNYVEVPECVGKDYRIAENIIINAGLIPEKVEQYNQDIPAGEVYA
                     VSPSETIKVKYGETVKIYVSLGSEFEKTTVPDLFGLTKEKAAGALLERNLIMNVMSEE
                     EVPGKESLVSYQYPAADTEVYENSTIDIKLSVDYGDTVYEDITVKYTFDPTIAELAGM
                     EMPITLRFKFEYSDQDEDFQTTAVISSIEKLPYQRDVRIPAKGETKMTVFIGSVIYKE
                     PIIYKYFDHYKETPEP"
     gene            complement(80875..81627)
                     /locus_tag="PLF14_00310"
     CDS             complement(80875..81627)
                     /locus_tag="PLF14_00310"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HOT69774.1"
                     /translation="MIFNYASYTDIGQVRGNNEDYHIVIPFDEHNEFAAAVADGMGGH
                     LFGEIASKTAIDCFEKKIRDRRFKNCNNIEDYLFNICKTANKEILDFSKKHNVFSGMG
                     TTLDAIYIKDDKLHVIHIGDPRIYTVYNNEIKMITKDHSYVQQLIDKGDITVDESHNH
                     PNRNIITKALGISDVPEPDIYDMTLDCCEYILLCTDGLTNMLDDTDILNIINQETDLE
                     EKVKDLAQKANEKGGTDNITIILIKADNNPEE"
     gene            complement(81617..82648)
                     /gene="rlmN"
                     /locus_tag="PLF14_00315"
     CDS             complement(81617..82648)
                     /gene="rlmN"
                     /locus_tag="PLF14_00315"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015615619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOT69775.1"
                     /translation="MSAPSIHILSYTHNELKDLFQEYGLKRYRALQVLDWIYNKKVFD
                     YSLMTNISKEDIEILQANFSLELPVIEKKLVSVDGTVKYLFRYSDNATVESVLMDYDY
                     GTSICISSQSGCKMGCVFCASSTVPFSRDLWGYEMTAQILKAAQDSNKTISRAVVMGI
                     GEPFDNYDNLVNFLNVINMKEGFNMGGRRITVSTCGIVPKIYEFADLCLQVNLSVSLH
                     EVTDDRRSAIMPINKKYNISSLIKACEYYFEKTNRRVSFEYALIPGSNDSLEHARRLS
                     ALLKNVMCYVNVIPVNPVENMKTVQNSKNLSPFCAILKAHGVPVTIRRELGRDIEAAC
                     GQLRRQHDI"
     gene            complement(82696..83646)
                     /gene="fmt"
                     /locus_tag="PLF14_00320"
     CDS             complement(82696..83646)
                     /gene="fmt"
                     /locus_tag="PLF14_00320"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOT69776.1"
                     /translation="MKKINNIIFMGTPDFALPCLIALIENGYTVNAVITQPDRPKGRH
                     NVVEYSNIKKKAIEYDIPVLQPEKIRKKEWVETISKIGCDLAVTCAFGQILPQSILDI
                     PKYGTINVHASLLPKYRGASPMQRAIIEGEKVTGVTTMLTDIGMDTGDMLLKMETDIT
                     EEDDICSLHDRLSLMGAKLLIDTVKLYEQDKITPVKQDNDKATYAPMIKKEEGRLDFN
                     LDSYRLFNLVRAFNPWPGTFTTYCGKTLKVLKADYSLEDYNKDVDNGTVISIDKERMA
                     VKCKKGILYITELQFENKKAMHISKCGHNMPCDMVLGCDE"
     gene            complement(83630..84124)
                     /gene="def"
                     /locus_tag="PLF14_00325"
     CDS             complement(83630..84124)
                     /gene="def"
                     /locus_tag="PLF14_00325"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOT69777.1"
                     /translation="MALRTIRKEGDEILRKKSREVTSFDDRLHTLIDDMYETMVAAPG
                     LGLAAVQVGVLRRVAVIEIDDKKYELINPVIIQSEGEDIDVEGCLSLPDYYGTVKRPM
                     KIKVRYLDRYGKEQYAEAEGYAAHCFCHELDHMDGILFRDKVIEEIDPADLEEREKQS
                     EEDK"
     gene            complement(84192..85295)
                     /locus_tag="PLF14_00330"
     CDS             complement(84192..85295)
                     /locus_tag="PLF14_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT69778.1"
                     /translation="MNTTNLDKVVQAYIDNFDYVNGDEHQEYYKWEAFKHFKDNWDID
                     AENFVEMFKESVKLTYNLIDNKAVSPTNGIVKLAERSELTEVIREMFRMLFIADDGNI
                     VQRQKRIEDFRDRVNDLLDKYEPGKWRYQHDFRTVLSYLNMRYPKENYLFKATEAKEF
                     MYCIEYPDDFGSGKSFNLSRYYRMCDALLEKIKDTPELMQAHKNRLTDTMLSDDDYHI
                     MVFDIIYCVTVYKLYADIAIKKPVKKSSVKQQRNAKAEELNLELDSVNAELDTALIER
                     SKIDDIDVVGWKIRHKVFGPGTVISQDEDRIIVKFAKGEKKFELPGAFSDGYLICEDQ
                     DIIEIFSEIDRLDKKISSLRSSINRITSTLSRL"
     gene            complement(85310..86233)
                     /locus_tag="PLF14_00335"
     CDS             complement(85310..86233)
                     /locus_tag="PLF14_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014357284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOT69779.1"
                     /translation="MEYLTVTQAAEKWNVSTRRVRLLCANGEIEGVIRKGNLYMIPCD
                     TEKPLDKRKLPHKRKHGKFASLLTSIDIKKVKLDSMRPLTAGEIKRLRNDFMVNFTYN
                     SNAIEGNTLTLKETAMVLEGMTIDKKPLKDHLEAVGHRDAFQYIEDIAQNKVRLKDTE
                     IRAIHSLVLMDRPDDKGVYRRIPVTITGAYAVPVQPYLIEPKMTDLLTRNEERKKTMH
                     PIERIARFHLEFEGIHPFIDGNGRTGRLILNLELIRNGYPAINVKFTDRKRYYDAFDA
                     FYRDRKADDMVLLIAEYVGEQLDRYLEIVKF"
     gene            complement(86422..87546)
                     /gene="ugpC"
                     /locus_tag="PLF14_00340"
     CDS             complement(86422..87546)
                     /gene="ugpC"
                     /locus_tag="PLF14_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HOT69780.1"
                     /translation="MAKVILKNVYKKYEPNRGGITAVSDFNLVIEDREFLVLLGPSGC
                     GKTTTLRMIAGLEDITAGEIYIGDRIINEVHPKDRDIAMVFQNYALYPHMTIFDNMAF
                     GLKLRKMPKNEIRHRVHAVAKILEIQHLLDRKPKALSGGQRQRVALGRAIVREPKVFL
                     MDEPLSNLDAKLRAQMRVELTKLHQRLKTTFIYVTHDQVEAMTMGTRIVVMRAGFIQQ
                     VDPPQIMYDNPVNMYVAGFIGTPQMNFLNATIEEKGKDVWIDIGGTKLKFSEKRAKSI
                     IGKGYDGVEVILGIRPEHFRDEQVFLDQMPETTIEVKIDVVEMMGPEALLYFSFADSN
                     IIARVSPSTKARPGSTIKVAIDTVRVHIFDKKSEKTIFAE"
     gene            complement(87661..88266)
                     /locus_tag="PLF14_00345"
     CDS             complement(87661..88266)
                     /locus_tag="PLF14_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOT69781.1"
                     /translation="MEQHKTKLMTTRNMAKMGILGAVAGVLMLLEIPLPFAPAFYEID
                     LSEVAVFLGTFAMGPVAGIIIEAVKILVNLVITGTATAGVGEFANFLIGCCLVVPAGI
                     IYKRKKNIKNAIIGMLVGIVSMTVAGALLNYFLLIPTYAKAFGMPVEAIVEMGTKINP
                     NINSLWDLILLANVPFNLLKGVLSGVITFLIYKRLSPILHK"
     gene            88406..89371
                     /locus_tag="PLF14_00350"
     CDS             88406..89371
                     /locus_tag="PLF14_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012060994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOT69782.1"
                     /translation="MKLGVFTVVLSSMSLENAIIYLKDLGVQAIELGTGGFPGTAHLD
                     AKKIYNKPEEIKKIKKLTEKYDMEISALSCHGNAVHPDKKIAAAYQEDFEKTIILAEK
                     LGVNTVVTFSGCPGDHDNAKYPNWVTCPWPDDFLKILDYQWNTVLIPYWTKQVEFAKK
                     HNVHQIALEMHPGFCVYNPETCLKLRDAVGPVIGANFDPSHLFWQGIDIIKAIRALKG
                     AIYHFHAKDTKIYYPNSQVNGVLDTKPYSDEPQRSWLFRTVGTGHDRQVWNDIFAELR
                     MAEYDHVISIEHEDSLMSGQEGLKKAISFLKETMIFEPKGTMYWA"
     gene            complement(89410..91332)
                     /gene="metG"
                     /locus_tag="PLF14_00355"
     CDS             complement(89410..91332)
                     /gene="metG"
                     /locus_tag="PLF14_00355"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOT69783.1"
                     /translation="MKKFYITTAIAYASGKPHIGNTYEIILADSIARFMRMLGYDVFF
                     MTGTDEHGQKIEEKAKEQNITPKQFVDGWAKEIKRIWDLMNTSYDKFIRTTDADHEQQ
                     IRKIFKKLYDKGDIYKSQYEGWYCTPCESFWTQSQLEDGKCPDCKREVNKAKEEAYFF
                     RLSKYQDRLMEHIERNPDFIQPESRKNEMVNNFLKPGLQDLCVSRTSFKWGIPVPFDP
                     EHVVYVWIDALSNYITGIGYDADGNHSELFNKYWPCDVHVIGKDIVRFHTIYWPAMLM
                     ALDVPLPKTVLGHPWLLFGDGSKMSKSKGNILYADDLSALFGVDEVRYYVLSEMNYAN
                     DGIISHRQIIEKINSDLANVLGNLVNRTVTMQHKYFEGTVKKPASLLPENQADRELIE
                     LTEKTPDKVCDLMMRYKVSEAIHEIMTLAKRCNKYIDETLPWTLFKNEEDHERLKKVM
                     YNLLEGIRVVSVLLYPFMPSTSDKIISQLNTKETTFASTYTFGKLEDGIKLGKPEILF
                     NRIDTEKKLQEIEKSDKQKEQDLNVALIGIEDFAKIELKTAKIVSCEPVKGAKKLLKL
                     QVETDNKTRQIVSGIAEYYTCDELIGKTVVVITNLKPAVLRGETSEGMLLAASDKDRL
                     SLVTIDREMPTGSKIS"
     gene            complement(91332..92207)
                     /locus_tag="PLF14_00360"
     CDS             complement(91332..92207)
                     /locus_tag="PLF14_00360"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOT69784.1"
                     /translation="MYDVIIIGKGPAGAQAGIYLGRAGFKTLILYKDGGSLEKADYVE
                     NYYGFGKPVEAKKLLQEGVGQARRFNVEFKEDQVVMLLPSEHSVSVQGLKGEYVASYV
                     IIAAGMTRRSLNVPGLRDLEGKGISYCVTCDGFFFRNKKAAIIGYTDYAEHELNNLLM
                     FTKDVYLLTNGNNPEFDVPGEIPVIDKQIGRIYGQDSVEGVEFKDGSKQQFDGIFIAY
                     GTADASSFSLKSGIATEKGKIVVDSSKMTNIDRIYAAGDCINTYMQVATAVSDGALAA
                     RSVTERLKEDRKRSK"
     gene            complement(92225..93058)
                     /locus_tag="PLF14_00365"
     CDS             complement(92225..93058)
                     /locus_tag="PLF14_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT69785.1"
                     /translation="MNFFSNSLVYFIKKPLIPIYTACISLVCCIIMIFNPAKLLSKYY
                     SSFISDDIGDTVIMFSKWAYKYTHIPYILLGILALAVVLALLSSLVFSGYMNIIHLTV
                     KRIKTDFTHYLQGLKKGFFRCALVFFQMYLSLLLFVAFIPLAFTPFFILKNSVADAGH
                     DPTVVYILLAVLIFIIIAIFILIYMTFAFKFPSIFHFSRYPIEKANAAVNARYWRTFA
                     KSALLLLFLAGVLFVMYKIENKVLEFLVGFVLYTIYFSFFAVFPFYTFDKLIEPYRVN
                     S"
     gene            complement(93123..94742)
                     /locus_tag="PLF14_00370"
     CDS             complement(93123..94742)
                     /locus_tag="PLF14_00370"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT69786.1"
                     /translation="MIDIIIVDDEKAIREGIATSIPWQQHGINVCGVASGVSEALDLI
                     EKYMPNIVITDISMPEISGLDLLEIVNRTYPNIKVILISGYRDFEYARRAVTNNAFCY
                     LTKPLVAETLIQKVLEAKQEIDARLQEVRLNDNIRRKLRENIMVLKDNFFRLLMEGRT
                     RQRADIMDRAAIAEIDINYTNYIVCVAEFELAASQKKLNIYDFSFYKAAIMSTIEENL
                     EEIYKCYTFNLDNRIGILICSNQQIQRSILINKLNDVKSWVNKNMGLSLSIGIGNTCG
                     SVEKVALSYISAMDAMLYRVVLGKNVVVDAEQKINASKEKMVVEDFDNILKNSEEDMV
                     FALRNVNQEEAKKIIEQIMQASRQIAANDVHQKERIAFLLSVFIIRVIYSLEISNYRF
                     YNKENDLYNEINELISIDDMKIFMLGIINEIIHAIEESDSKQNNFLVNKGLRYIKESV
                     YEDVSLVTIAERLQIHPNYLSKIFKQVTEESFTDHVIRFKMNEAKKLLKTTNAKVYEI
                     SDKLGYKDVAHFTKLFKKHFGISPTEYRQLL"
     gene            complement(94735..96489)
                     /locus_tag="PLF14_00375"
     CDS             complement(94735..96489)
                     /locus_tag="PLF14_00375"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOT69787.1"
                     /translation="MLGVLVICLAIVLLLITANNIILAKIAERDKNRLLEETDRRIVL
                     MYTKQVEMFDSVASNIANSDMLSEYADNIYYLDDIMHDNSDIFRYFKLMSNTNEYIDH
                     IMIYDYELEYLLNGRNEFAAYVDKAGFPWFIQRVEGRIPSADLDKYCMWHIYDEYIAI
                     TYKADVLNPSLNRRVFITLNITQTDFARFLDGPVLTENSTNGFISKDDNFFFSGSEEI
                     LMYKTRGQAFDYFDDLLIKEKVTIEHNYVLYYLQYSPLDCDYDLGIVYLIPEKDIAND
                     VYKYTVIISIGVSLFILILCYSIFRRILETNVQRPISRLIRGMEKVGSGDFTSKLDDS
                     ICKDNEIKGLFHAYNSMTDRATVLIEELYQAELYRKQLELQTLRDKIDPHFLYNTLDT
                     INWTAKEHNVEEISKIVVALSTMYRKIFNRGQDLTTIKDALEGIECYLDIQRIRYGES
                     FSYHIFCPDELKECVILNLIIQTVVENAIIHGLEDRDSGILKIYVEHEKNDDDSKGDI
                     IIKVIDNGIGMTEEKLNLITSSINSAKLESESGLRNVQKRIKLYYGMQYGITIMSKYK
                     EGTTVILRVPYEERKEDD"
     gene            complement(96547..97599)
                     /locus_tag="PLF14_00380"
     CDS             complement(96547..97599)
                     /locus_tag="PLF14_00380"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT69788.1"
                     /translation="MDYKRTDEIIKRTKDMYESGKGALIQVKEIFSGIDVPFRPLDSF
                     GLPKNMETYLDYMIERQRIFWDTRNQIPDDIIQSITVRYGIAEHSAFMGGDVTFSETT
                     SWHHQHVKDYSDYTLAKVDESNIWRNLVIEGMKYMKSKVKDEFFIRFRGADGPLDVVN
                     ALRGNDIFYDFYDEEEGLLELTSKCTDAIIYMLKEQQKIVTEYKGYVISGFDVIMPKG
                     YAGHLSVDATTMLSDEMFRKFEVPFLERIGDNFDGLFFHVHSVGHHVIPAVCEVDKIK
                     FVEISNDPNAPRGIEVYKKYEKELSNKIVTVALTKEEIIDNLNFLRDKKVIVWYDASD
                     VEDAKKAVDLVRSKLG"
     gene            complement(97678..99006)
                     /locus_tag="PLF14_00385"
     CDS             complement(97678..99006)
                     /locus_tag="PLF14_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015554161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOT69789.1"
                     /translation="MYLKRKIDNFLEEWKANPDRMPLIVKGPRQVGKTQSVIQFGNVN
                     YKSVVYINFVEEPKYKLITADGYSAEGIIKNISRMDPSKQFIGGETLIIFDELQEFPD
                     IATSLKFFKIDGRFDVICSGSMLGINYHKIESNSVGYKSDYEMYSMDFEEFLWAKGYN
                     DGHIESMLENMKNLTPFNEVEMSVYNNHFLDFCILGGMPAIVREFIIKGTFEGSLELQ
                     RQLIIDYKEDIRKYAVGMDQTRILNVFNHIPIQLAKDNKKFQISKVASGARFRDYRGC
                     IEWLDDAGIINLCYCLNFPELPLRGNYDDTKYKIYFADTGMLVAMLDEEAQEDMRANR
                     NLGVYKGALYENIVGEALKKSGYSLYYYKRNDSTLEQDFFVRTAKSLIPIEVKAKGGT
                     AKSMRMLISEDRYSDISYGIKLTGGNIGYSEDIYTFPYFCTFLLKRYLSD"
     gene            complement(99281..100177)
                     /locus_tag="PLF14_00390"
     CDS             complement(99281..100177)
                     /locus_tag="PLF14_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOT69790.1"
                     /translation="MLKRSLGEKIFNIFNLLGMFLFTLIIVIPVLFVFRNSFELSETA
                     TATLRLWPEDFSLVFYGMVVKDETVYRPLLNSVIVTIVGTPLTLLINALGAYTMSKRE
                     LPGKDFFAYYMVIIPMLIGGGAIPSFLLMRALGLIDTLWVLIIPSLASGWNMTLLRNY
                     YWSIPNELTESARIDGASEMTIFVKIILPLSKPVLSALGLLSGLGFWNIFYAAMIYIR
                     TPRKWTFPVKIRETVMKNEGNDKLWEEMMVAAGFDPTELLINQSGLGGAMMVVSMLPV
                     LIVYPFLQKYFAGGIMVGSVKG"
     gene            complement(100194..101066)
                     /locus_tag="PLF14_00395"
     CDS             complement(100194..101066)
                     /locus_tag="PLF14_00395"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOT69791.1"
                     /translation="MIYLMIVPGLILYYIFRYVTMPGIMIAFQEFTTGGFKYWCGFEN
                     FDKLFGLRAFWQAFRNTWVFIGLQYLFIKPSPIILALLLNEVRIMWFKKTVQTVSTLP
                     SFLTWVVVSGIFIQLLSPSTGYVNVIIALLGGERIYFLGKPQLFPWLLTGIRIWKGVG
                     YSSIIYLASLGGVDPELYEAAVIDGATRWQQTMYITLPGIKNTVVVLLIFSFSGIMGG
                     LFEPVFTLTDGNALLDETAMVLDVYIYNTGIRQGKFSMSTTTGLFNSIISTILMLSAN
                     WASKYLTEDGRTII"
     gene            complement(101263..102258)
                     /locus_tag="PLF14_00400"
     CDS             complement(101263..102258)
                     /locus_tag="PLF14_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010294036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HOT69792.1"
                     /translation="MEILEKLTATFGVSGRESEINQVITDLVKDHVDRVMTDGIDNVI
                     AVKNGYGKDKKKIMVSAHKDEIGFMVMYIDSDGYIYLRPVGGIPVSVSIGNRIIFRNG
                     TVGVLCFKKDVESIKNNNVGDMFADIGAKNKEDAMNYVKPGDMGSYYGPLCMLKNDRV
                     VAKALDNRIGCYVAIKALTENTEPYNDMYYVFTSQEELGLKGAIVASRVVQPEIAVAV
                     DVTSSFDTPITKEGNAVLGGGAAIKVMDRSVICDESLIGIMQDICKKNDIANQSDILA
                     AGGTDAGAIYTSNQGVRTGGISIPMRYVHGPVGMVDMNDVKACIELLKGFANQKI"
     gene            complement(102283..103632)
                     /locus_tag="PLF14_00405"
     CDS             complement(102283..103632)
                     /locus_tag="PLF14_00405"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT69793.1"
                     /translation="MFGKIKDYLRKLYIAVFYRNDTKLLTMPGKKDRDWFIVQGFVGT
                     VFALLTGATYLTGLFNKVNAPEIVIAYLPIIGSVAGVMAIFAGYVMQTIKNRKKFTLI
                     LNTILKTLIVFIAWIPLIFPNQYASYIMIAIALIAYALNNFMGIAINSWFVDLIDVKI
                     RGQYMSVRNMIALIAHLLVPIIVSAFIDTSSDQYMAIVIVFTISWIVMWAENYSFYKI
                     YEPPLQPPKKKIKLKDVLIVPLKDRTFMKSIASIAVFYFIWYLSMSFATLYQLNYLGL
                     SYTFISISATLNVILQFVWYPIVGKLVDRHNTDFLLIVSYILYLIQAILWGFTNISSR
                     YVMTILVNIFASMAVPLFSLVVFKAKYDVIPAEDRSLYDGFYTALVALILAVAPAIGN
                     LMKDFIAVNMQPFGVFEVPQFQLIFMLSGILLLGLILFNMKDTVKLFREAKERKTEN"
     gene            complement(103634..104389)
                     /gene="map"
                     /locus_tag="PLF14_00410"
     CDS             complement(103634..104389)
                     /gene="map"
                     /locus_tag="PLF14_00410"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003446049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOT69794.1"
                     /translation="MTNIRTKSQIEMIREAGKIVAACHENLRKIIKPGITTFAIDRFV
                     EEFIRLHNAIPAQKGYHGYPYASCTSVNDEICHGMPSGYVLKEGDIVKVDMVADKDGW
                     MADSAWCYAVGEVSPVAQKLMTVTKECLYKGISKAVEGNRLGDIGYHIQRHAEKNGFS
                     VVRDFTGHGIGRAMHEDPAILHYGQIGRGSRIVEGMVFTVEPMINEGDYDLYVDEDNG
                     WTAYTADGSLSAQYEHTVAITANGPEIMTKQEF"
     gene            complement(104420..105568)
                     /locus_tag="PLF14_00415"
     CDS             complement(104420..105568)
                     /locus_tag="PLF14_00415"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOT69795.1"
                     /translation="MKGRKRKITMAVLLLLLAINLTSCALITLIKKNDATPGNSPFMQ
                     KISFNPYSVGICLEDVNTDHAKYVADIDEFYIHFDSCVAKKHRSIFVRSSSQDNFSSL
                     DCLDKYGLKGCYRTIYSTNSGSYFAIYELEYYPGDSIVYAYLNNDRSLLSQKELEIYR
                     FAVDFVNNRLDSKMSDYDKEIIIHDYICETLGYQKEADNDADKYNITGAYCLTTGFAN
                     CQGYTDAFYMLTNMAGIRCQKVSGEAQGTAHTWNVVNIDGRWSYVDVTFDDTAFDSDE
                     VIFYAYCNVPEEYLEKSHIFSEDQILFPTTGYEDFYYKRKGNAVFNKEDFRQIVYLPI
                     SEGATRVEAFILIKEIQPLLDTLKDLNKSMQLSYFEINECTLLDITVQ"
     gene            105767..106837
                     /locus_tag="PLF14_00420"
     CDS             105767..106837
                     /locus_tag="PLF14_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOT69796.1"
                     /translation="MILKKDCKYDIMAVTSMGIRITPVNRQPVNVSNLYEMHATSAET
                     NVLNISAALGLKAKVLTKFVKDSPIALYIKGELRKRNIEFEGIDIEQGGPWGYRHQFN
                     IADSGFGVRGPRVLNDRAGEVGRTLSASDFDLERIFKEEGCRILHLSGLIAALSEETS
                     KCCLDIALAAKKYGTAISFDLNYRASFWKGREKELREIFTKIASISDILIGNEEDFQL
                     ALGVKGPEAGGKSISKTDAFKDMITRISAQFKNVKVFANTLRQVENANLHLWGAIIYA
                     EGNWYVVEPREIPVLDRIGGGDGFVGGLLYGILKGWQPEKWVQFAWASGALATTLLTD
                     YAHPADEEVIWSIYEGNARVKR"
     gene            complement(106944..107147)
                     /locus_tag="PLF14_00425"
     CDS             complement(106944..107147)
                     /locus_tag="PLF14_00425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HOT69797.1"
                     /translation="MNVPKIVPIAELRDTKHVLQMCRAAKEPIIVTKNGYGELVIMSI
                     ATFTEMTEELNRLRKQVAKDSTY"
     gene            complement(107287..107991)
                     /locus_tag="PLF14_00430"
     CDS             complement(107287..107991)
                     /locus_tag="PLF14_00430"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HOT69798.1"
                     /translation="MNGSDSLTERFILEMEKKIVNGEYKINTMLPSFRKLCDEYNVSR
                     SVINTAVAVLNTKGFVDIVPRKGARVRNWKKQGTLLTLNCAVAFGKLEQEIIDSFFDI
                     FAFIECNGVYEAALKATEEELVQLGNIAEQEVLLKDEKKLAALYHEFYKKIADISGNM
                     AYSQIFMTLEQAIFAVSDIYVSDAGAKQQCMKMHSNIYQSLKARDGQKARWEMQALSD
                     HLKVLVEYKTSNLKRK"
     gene            complement(107988..109436)
                     /gene="gcvPB"
                     /locus_tag="PLF14_00435"
     CDS             complement(107988..109436)
                     /gene="gcvPB"
                     /locus_tag="PLF14_00435"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOT69799.1"
                     /translation="MAELIFNLSKEGKCAFSLPESQVPSYEIDKKYTREDDVSLPEVS
                     EVDVIRHFTNISRNNYGVDQGFYPLGSCTMKYNPKVNEDVAGLQGFTSLHPYQPEYQV
                     QGALKAAYELGGYLCEITGMKGATLQPAAGAHGEMTGIMIIKAYHQNRNDNKRTKMIV
                     PDSAHGTNPASAATAGYEIVQVKSDDLGLVDIDALAALMDDETAGLMLTNPNTLGLFE
                     SNITKIAKIVHDKGGLLYYDGANLNAIMGIARPFDMGFDVVHFNLHKTMSTPHGGGGP
                     GSGPVCVREDLVRFLPKPVVCKDGERYYLDHDRKDAIGRVKAFYGNYLVYIKALAYIL
                     TMGPDGLKKASETAVLNANYIMAGIKDSFVIPYDRFCMHEFVLSGSRQKEQYGISTLD
                     MAKRLLDYGYHPPTIYFPLIVHEGMMIEPTETESKETLDKFIEALNKIAKEAKTNPEL
                     LHDAPHNTYVRRLDEVTAARNPVLKWEKEQDE"
     gene            complement(109429..110736)
                     /gene="gcvPA"
                     /locus_tag="PLF14_00440"
     CDS             complement(109429..110736)
                     /gene="gcvPA"
                     /locus_tag="PLF14_00440"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOT69800.1"
                     /translation="MMRYIPVTDRDRQEMLKRCKADSVEHLFSCIPEQVRLKRPLNLP
                     PAKSEMELIKYMQSLAAQNSNTGNYACFLGAGAYDHYIPSVISHLISRQEFLTAYTPY
                     QPEISQGTLTAIFEYQTMICELTGMDVANASMYDGASALAEACAISSGAFRKRSDIII
                     VEDMHPEYRETVETYLKFRDIKYTSMTVKQLLEADLTNVAGVCVQYPSFYGDIYDMQV
                     IGDYVKQNGSLFIVCANPVALALLKSPKDFGADFVVGEGQPLGMPMGFGGPYLGFFAC
                     VKEHMRKMPGRIVGKTTDVDGKTAYVLTLQAREQHIRREKATSNICSNQALCALTATI
                     YLSVTGKKGFKQVAQRCVDNAHYVYEGLLKTGKFEKTSDMPFFHEFTVKPKVNIAALN
                     RYLYGHGFIGGLETDRGWLIAVTEKRTKEEMDEFIRRAGEFNG"
     gene            complement(110733..111101)
                     /gene="gcvH"
                     /locus_tag="PLF14_00445"
     CDS             complement(110733..111101)
                     /gene="gcvH"
                     /locus_tag="PLF14_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOT69801.1"
                     /translation="MRFTRDHEWIKKEGKYAYIGITDYAQQQLGDIVYVELAEEDLVL
                     QQGDVLCAIESVKTAAEVYMPVSGTVIEANAELEDAPEKINEDPYGTYLAKIIIDDET
                     ELNDLMTEDEYNEFLESEAE"
     gene            complement(111115..112203)
                     /gene="gcvT"
                     /locus_tag="PLF14_00450"
     CDS             complement(111115..112203)
                     /gene="gcvT"
                     /locus_tag="PLF14_00450"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOT69802.1"
                     /translation="MKTTPLTSVHESLRAKMVDFGGFYMPVQYTSIIEEHNAVRNKAG
                     IFDVSHMGEISVTGKDAIKYLNYIITNDISVISENQIMYSPMCYEHGGCVDDILIYKN
                     NDKDFLVVANASNTDKDFEWMKKHTKGYDVVVSNQSDRYGQIALQGPEAQNILQQLTK
                     TDLEEIAFYRFKNNVVVDGVNTMVSRTGYTGEDGFEIYTAAHDTEKLFRKFMESGAAP
                     CGLGARDTLRFESALPLYGHELSEEITPLNAGLKSFVKPEKSDFIGKAALIEQTEKGV
                     SVRSAGLEMVDRGIARNGYDVFNEEGEKIGYVTSGSNCPSLGKNCALALISTEYTKVG
                     TFVYVDIRGKKVKAVTVKKPFYKKMYKK"
     gene            complement(112274..113332)
                     /locus_tag="PLF14_00455"
     CDS             complement(112274..113332)
                     /locus_tag="PLF14_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT69803.1"
                     /translation="MSYELGMQAVNLEMPDIVPRTEYSYQLAYELLHAVTGIAVNKDS
                     DDDTKFNAITAFERAWDISLFWATGIGSNIFGDKRTTMGHAGFEEGYGDYRDNKHEAF
                     TDIEEIYNLDFYETYGTIDLKTKAKAITEGYLERCQLHPDGVNTSGIYVTCISGILEI
                     LGWDMLLSAAGEDPARFGEFTVRYSQWISQYFKALAKSDVPVCMIHDDMVWSSGPFIA
                     PAWYDKYVFPYVKKNISYLKEAGKKVLFTCDGGFDLFVDRFVDMGVDCLIFEPLTDLK
                     YICERYGKTVSIIGNVDTRIIMRGSREDIENEVKRCMDLGKPCPGYMLAIGNHITPGH
                     NVENYLYYNEVYEKLKRR"
     gene            113423..114427
                     /locus_tag="PLF14_00460"
     CDS             113423..114427
                     /locus_tag="PLF14_00460"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HOT69804.1"
                     /translation="MERLMFISYLKVPTLISNKRYKRKGVCDMTTLIAGLIISIALLS
                     VIVYFNSKKDSKKKFRANCIVAALPLIIAFFIASIAVIPANSVGVQYSPFKGVLEETL
                     PEGWHFKGVFDNIYIISTEVQTSTLTEITGQTKDSQYVEMVIDVKYKVSPEKAYEVFK
                     QYRTLENVAVSLVSPTVQRSIETISIQYNIIEILGEKRNELYAGIEEELTKRFAINGI
                     QFVSINFTDTDAGAEIEKAIQDEAIAKKRVETAEQERLRVEIEAQQRVIEASANKEKA
                     EIEAQIKLIEAQAEAEANRLIAESITQELLQKMEMEARLKWGWVTVQGGSVITDIREK
                     "
     gene            114470..114934
                     /locus_tag="PLF14_00465"
     CDS             114470..114934
                     /locus_tag="PLF14_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT69805.1"
                     /translation="MEFGWINVFGAGIVLFLMIPNIVYAVKNKNEKNVCTDKYMNITE
                     QIGRYACIILMWLPLLVWKFGFASVFEMLLYIIGNGVLLIAYWSVFALYLKKKNKNRA
                     LILAILPACIFFFSGLLLRHWLLVGFSVIFAIGHIYVTLKQFSPTTTVQNDK"
     gene            114939..115436
                     /locus_tag="PLF14_00470"
     CDS             114939..115436
                     /locus_tag="PLF14_00470"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012290235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HOT69806.1"
                     /translation="MTKHQTVTHPLQPLFNDDSKILILGSFPSVKTREYGFFYGHPKN
                     RFWPLIERIFDVKLSTDIEERRTFLLQHHIAVYDVIYQCDIIGSGDSSIQNVIPSDLS
                     RIFKEADIKKVFCNGATAYHYYQKYQEQKTNAKAIQLPSTSPANARYSMDDLYNQWKV
                     INYIL"
     gene            115633..116037
                     /locus_tag="PLF14_00475"
     CDS             115633..116037
                     /locus_tag="PLF14_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009537151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOT69807.1"
                     /translation="MKYVYPAIFTVEEDGGYSVNFPDFESCYTCGDTLAESLAMAEDV
                     LAFTLYKLETEQALLPAPGKLQGLSLGENEFANYIACDTMEYRKRKNSKAVKKTLTIP
                     SWLNEEGIARGINFSQVLQDALMEKIEKYTKT"
     gene            116122..117393
                     /locus_tag="PLF14_00480"
     CDS             116122..117393
                     /locus_tag="PLF14_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOT69808.1"
                     /translation="MKTYLPRIIDKLLKERLEAKGAVLLEGPKWCGKTTSAKEIAASF
                     ISIDQPEMTKQYQSMAELSPLQLLEGETPRLIDEWQIAPNLWNAVRYEVDQRDEFGQF
                     ILTGSAVPAEIDKSMHTGTGRISRLYMRPMSLYESLDSSGKVSISDLFASREISAKSD
                     TDLEEIAFLICRGGWPKSVGLPENPALYQARDYFEAVVSTDISRVDSVRRDKEKAKRI
                     LRSYARNVGTKTSLETIRQDVLANHAGTLDPVTLYSYRDALTKIFVIEDSPAWNPNLR
                     SKVAIRTTDTRYFVDPSIAAAALELGPVDLLNDLSTMGLIFENLCVRDLRIYADYLDG
                     SIYQYRDSNGLECDAVIHLRNGSYGLIEIKLGGDSLIEEGVNNLNKLASNIDTRKMKE
                     PAFMLVLCAKAPFAYRRKDGVYVVPITCLKP"
     gene            117602..117898
                     /locus_tag="PLF14_00485"
     CDS             117602..117898
                     /locus_tag="PLF14_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT69809.1"
                     /translation="MQYDLMSIISKRRNELISVILVKCNVMEAILFGALKNWKQEEIF
                     YKAKFAKVVMRKNGQFQNFKKIFWHNRIHVKAMYRMSIATFGTGVIPNIFKLKL"
     gene            complement(118034..119017)
                     /locus_tag="PLF14_00490"
     CDS             complement(118034..119017)
                     /locus_tag="PLF14_00490"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing transcriptional regulator"
                     /protein_id="HOT69810.1"
                     /translation="MAKSAVQKAKLLHIYKMLYENTDENHVMTVNDIIRQLATVGINA
                     ERKSIYDDIKVLNELGCDIIGVKSKTYGYYMASREFQIPELKLIIDALVAARFLTEKK
                     TKELIDGLCNLASQHQKKELVRNTGVYSKAKSKNENVYYNIDSINEAISKNAKIRFSY
                     FDYDIDKNKVYRGVRGKYKVSPYALIWEDECYYLVCYYERYNVTQFKVDKMESIEILK
                     EKIDMDKKEEFDLVTYTKPMFNMFKGEPVKAEIIFDMSLLNVVFDRFGLDITVYPVGK
                     NKFKAFLNVSISPTFIAWLFTFGDKAQLVAPENLVDEIKSTLNSLNKTYGC"
     gene            complement(119031..119504)
                     /locus_tag="PLF14_00495"
     CDS             complement(119031..119504)
                     /locus_tag="PLF14_00495"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - cellular response to DNA damage
                     stimulus [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HOT69811.1"
                     /translation="MVCGKESVMCGRFLNLEEREIFPSDLVEIETIQGNMDKIWGVVN
                     KYNNKTVINARGETVNELTMFKYMKPCIIPATGYFEWDKDKKKYLFTKPDRSVIYMAG
                     VYKEDRFVIITTEAYEQFMSIHHRMPYIISIADIPAWLKDRRLLNRREEYIYKRA"
     gene            complement(119480..119731)
                     /locus_tag="PLF14_00500"
     CDS             complement(119480..119731)
                     /locus_tag="PLF14_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT69812.1"
                     /translation="MTEDKRVKEYVNVTVKFKKDGQMIPVNIEWEDGRIYTVDRVLDI
                     RRAASLKAGGCGVRYLCKIMGTETYIFFEEDKWFVERKA"
     gene            complement(119728..120984)
                     /locus_tag="PLF14_00505"
     CDS             complement(119728..120984)
                     /locus_tag="PLF14_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOT69813.1"
                     /translation="METIILHADLNSFYASVECLYNPAIRNLPVAVCGSVENRHGIVL
                     AKNQQAKAVGIRTGEAVWQAKLKAANLVVVPPHYDRYLRFGNLAREIYCSYSEKVQPF
                     GMDEAWIDISFPGRTMDQGYEIAEKIRRRIKFELGITVSIGVSFNKVFSKLGSDMKKP
                     DAVTVIDRDNYKDKVWPLDVGELLYVGYATKRKLNSWNINTIGELADIDAVFIREKLG
                     KCGTILKDYAMGNDYSPVENTQHETIIKSIGNSTTPPKDLCSTEDIKITSFVLAESVA
                     RRLREHGLKGKTVQIYIRDCDLESFERQGQLSDYTNISSEIADRAMKLVKANWYFNKP
                     VRSFGIRVTDLASSKGPIQISIFKEENKRFKTMDLEYSIDSIRKRYGPYAVQRAVLIH
                     GRDCGDLSDSTEALMRKVSFNTGEKR"
     gene            complement(121109..121513)
                     /locus_tag="PLF14_00510"
     CDS             complement(121109..121513)
                     /locus_tag="PLF14_00510"
                     /inference="COORDINATES: protein motif:HMM:NF025833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HOT69814.1"
                     /translation="MKRTVYIIFLLCLIFVLITGCTKDTDLSEGYVYVTIDATKADKG
                     IILSEYAVSLKEGTTVFDVTLAACKDNKIHMSSRGTKVTRYVEGIAGLYEFDCGPTSG
                     WIYYVNGVSPSVGSDTYEIEDKDQIKWEYIIE"
     gene            complement(121513..122925)
                     /locus_tag="PLF14_00515"
     CDS             complement(121513..122925)
                     /locus_tag="PLF14_00515"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOT69815.1"
                     /translation="MRKALAVILAVCVLLGAVCVKASEDTCEKISQLYLEKAELSLWE
                     ALGLSLNGYRIPDSYYAALKEDIVSRNGVYRTSLEYAKIIILLKQDGKNPKDFHGYNL
                     VSLLQSFKKIDKSGVNGVIFALFALTDVQSEDNAIWTKDNLLTLLLEYQNEDGGFPLA
                     KGWGSDSDLTAMAVSVLAYYQNNKQAKEAMDKALSYLSGKLDNDGFMYYQKFDSSEIL
                     SQIIIALCTAGISLNDSRFVRNGKTLYDTLIENYMTKDFRFKHSISQSADAMATEQAY
                     LALSAMKTGYVYVKKEKSVIPEPSPSPSPIASPSSTVPPNIIHEPTDPAVVRKTGFFD
                     EESISKRYYSDVLTAYTEKLVVGDGNNLRPLDNLTRAEACVMLYNVAPLDTDIFVPRF
                     KDICRESWYSKYVLACYLNGIMFGADEVYFEPDSYITVFELTESISRIAGIDVVNDDS
                     FITREAAISLMVKIFYGESY"
     gene            complement(122926..124755)
                     /locus_tag="PLF14_00520"
     CDS             complement(122926..124755)
                     /locus_tag="PLF14_00520"
                     /inference="COORDINATES: protein motif:HMM:NF025833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HOT69816.1"
                     /translation="MRRSTKTICMIVITALLIAMTPAVFAAQTEYAYIRIEGYEGTLV
                     EKTAVPLNRFDLKAYGVTEEPSDYTVLHAVIYALKEKGHDPSKAKVLEANGGSYISSV
                     LGLKAEGNAGWMYVLNDESSWNSVKDQKLEKDDYVVLYYIKDWMNCSYSTFGVADEYE
                     VDAFTTVKLNLKHQVTDPVTWAASNENLAGAVITVDGKKTDAVTDEFGNAYLTFKDKG
                     THIVSAEKYVDGINTISRPYAYIDVQGKGVAVNIYTTDKTYRACVEPKGFDLAKYDAS
                     SECEFSALHAIIAALNSNGFDAADPKIANYNKGSFISALAGVEASGNSAWMYTVNNQT
                     SWFAVNEQPLTDGDVLDIYLLNDWATDTYSYFDMTSIKVQGGENIRVRLYRHVLDFVT
                     WETSVHPYANCEITVNGHKAGYVTDNDGYVNLKFNKKGVYEISVLPKEDNISRASVTV
                     DVTSDSDVSLEQYNDYAEVTDNYLTYMEKAVGKYIMNGDQDNNLRPADEITKAEFLTM
                     LVRASKLDVPGKYRKRYVDVVAGNWFGGYAQAVFRYGLAKHDKGYLYPNAAVAADVAE
                     YALNTAFASDAALSDAQKAVFTEGLTREEAAYLIISYMEAIGK"
     regulatory      complement(124838..125011)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(125029..125784)
                     /locus_tag="PLF14_00525"
     CDS             complement(125029..125784)
                     /locus_tag="PLF14_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT69817.1"
                     /translation="MVGRINIIIMAISAGAFVILPLVFSVVMIIRKKTKSMLLGMASF
                     SIYGIVVKNLIFSLIFLVPFLKTRLSSDYIVYSLFIGITSALIHMVTRYALHIKIGIK
                     DKAAFSAGESVIQCIYVGIPTTIIGLGYAVAINLGGFEYVGITNKVLPALTSLINADY
                     TEYLPYVIYPFFVFALNRMTVDLFIKNKFVSALTEAVILSIYSYFMLCRLYIPGTIFL
                     LAVTVVIVVVLIVSASKKQVGKRLDSVDTTTLL"
     gene            complement(125788..126567)
                     /locus_tag="PLF14_00530"
     CDS             complement(125788..126567)
                     /locus_tag="PLF14_00530"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HOT69818.1"
                     /translation="MGASVINCKFSSNLLYYQDECNVILPSLAKPEDLKVLYLLHGMH
                     GTCHDWITYTNIVRLAENYRVAVVCPNAKNSFYADMRYGEPFYTYLTTEFMEYVQRTF
                     GFSSRRKDNLIAGLSMGGYGALKIAFNNPDKFCIVGSFSGVLDMANIKGFVTGDEQTD
                     RKNIQLFISIFGEDPVLAGTDNDLISLIQRNKTVNGYSVKDLLVYQYCGTSDFLYLHN
                     MSFKKCAEEAKLNLKYATDGGAHDWERWDEQIALFLQEYFG"
     gene            complement(126628..127806)
                     /locus_tag="PLF14_00535"
     CDS             complement(126628..127806)
                     /locus_tag="PLF14_00535"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HOT69819.1"
                     /translation="MDFYSNLRNKLQAMKDGGTYKEYQYLTTPMSNKSDIEKYDEVIV
                     LCSNNYIGLCNNKEVIQAGHEALDKYGAGAASVRFICGTYDIHRQLEKETAEFMHTES
                     CLTYMSCWNANTGVIPAIVFEGDCIISDALNHASIIDGCRLTSRKVERFVYKHSDMED
                     LERLLKEGERFNTRLVITDGVFSMEGDIAKLDEIYKLCKKYKAILLVDDSHGMGVVGA
                     TGRGAIEYHNLLGKVDIITGTYGKALGGSAGGFVAAKKEVIDMCIQASRTSLFTNSLP
                     PVVAAMSLKAIQLLKRDNSIIESLHKKVKYLRQRLHEEGFEPLEGESAIVPIIVGDTA
                     KAIRIAKAMLEKKVYVTGFGFPVVPEGTARIRIQVSDALSYEDIDYSVKAFKEVFDNL
                     "
     gene            complement(127893..129221)
                     /locus_tag="PLF14_00540"
     CDS             complement(127893..129221)
                     /locus_tag="PLF14_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006906795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HOT69820.1"
                     /translation="MKYYESNRLELKEKLNDKFIRAVVAFLNTDGGRILIGVTDDGTV
                     VGVNEIDKTLKQIADIITAQIEPSPTDSVKTEILMEEGKVLVSVSVLKGTKPIYCIKK
                     YGFSSIGCPVRIGSTCREMTEEQIANRYKLKFLNNDLITEAVTNLPLLSFRSLKQYYL
                     DKGYRLNDDNFETNLYLIAPSGKYNIMGELLSDNNRYSLIYVKFRGLDKASISQRTDY
                     GQRSLIFAYEQLMNRIASENICTTDTTIRPRKDTYLYDYDCVKEAVINAIIHNDWLIS
                     EPQVSFYTDRLEVFSHGGLPHGLTKEEFFRGISKPRNLRLMKIFSDLDIVEHTGHGVP
                     TIIRKYGKEVFDITDNHIMVTIPFERSVLNEVIQNNVGINVATNVDINKTEKDIMEII
                     LREPRATYGVIAKKIEKTPKTVERNLSKLKEKGYVIRQGSKKSGWWKVIK"
     gene            complement(129429..139958)
                     /locus_tag="PLF14_00545"
     CDS             complement(129429..139958)
                     /locus_tag="PLF14_00545"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HOT69821.1"
                     /translation="MVRGGGVNSIIIRGSSTIAKVVVSRADGEVRIEVDDESNVEVIY
                     IDDGSDDVIITGSVQTLDVVANDITVVAREAKIESTTITGENCNLIVNKDSEINKVEI
                     AAQNANVSGKGTVKEAKVQEDGSNSKIETPNTKIQVDSKAKDVKGAGGKTIGGGSTAT
                     NNSTGTDTTGQTPFIPPYTPPYTPPVTPTYTPVSGVNITGTTTVGQMLTATVNPSSAT
                     VSYRWQRAESSDGEYKNIQGATARTYTLTDDDANKYVKVIATGSGSYTGTVSNSTSEP
                     VKQITYTVTFKDWNDTVLDTQTVEKGKDAVAPTVPGREGYVFINWNILFNNVTRDLIV
                     TALYEINKYTLSFDSNSGSLVDSIRNIEHNSTVTLPANPTKEGLHFAGWFVDNNVFNI
                     AFTESTVVTSNLTVYAKWVTELIPIAGVSLNKKVTAILPGATEQLTATALPANADNKA
                     VTWRSDDDSIASVDSEGLVTGHSVGKTTITATTVDGNKIAQCTIVVYTNSVEKDFEFD
                     AYNKLITGYKGNGGNVTIPSIINEVEVFKIAESVFADNDTIVSVTIPNTVTEIQANAF
                     KNCVNLVSVNHQVGSFLEKIGDYAFFGCINLASFDIPKKVREIGKSIFGDCRSLLEIA
                     VSETNADYSSIDGVLYSKDGSTILTYPAGITAASFTISDSVTSIGEGAFWYCRNLKEL
                     LTSENNASYRSVDGILYNKNNTSLVVYPGGRTETSYTVPRNITQIGRGAFGYAYNLLN
                     IYVEEGSLSFSSIDGVLYSKDGKTLIAYPTGRPDAAFSIPLNTRTIGEGAFMASVNLT
                     DIDFQTSPQGRRASIMCSLRGMNPESPVIESYAFYECSNLVNISIPSSIVSIGDYAFA
                     NCANLTSVTFSESSNLSHIGIGAFSGCPKLYSFVIPNSVTSIGNDLFSCSTGLTNVTF
                     QEDSQLTYISDAMFSCCFSLNSIEIPSSIVNIGEYAFYGSGLWKIIIPDTVVNIGSNA
                     FGGYYLCLVEIGSGVQIADNAFFNQDHFRNAYTSGGAGTYVKQYRADNIWRKTQEITN
                     VTGISLAKSEIRIPAGGMNWTYRVTATIQPFDATIGYIRWSSSDEAVVLVNGDGRMKG
                     ISAGTAVITASTVDGNYEASFTVTVYEPIVEGDFWFEAETGTIVHYTGTQDKVIIPET
                     ISGIPVQAIGQEAFCGNLHIASVTIPSSVERIEDGAFLYCWNLNVVDIGSSVQVADDA
                     FIYYHYYFLETYTTGGAGTYARDDYWGWRKIDHLINVTGISLERDEMRIPAGGWYSIP
                     NTIIQPSNASIKGVVFTSDNEAVVSVNGDRHMRGNSAGTAIITAKTVEGGFEATCTVT
                     VYEPVFEGDFGIEPETGTIVLHTGRQSRLTIPEIISGIPVQAIGEGAFSDMYITYVSI
                     PSDVRVEKNAFNCVNLYAIKFGSNVQLEDEAIGGDSLCDAYATGGAGTYARDGWRWNK
                     IDNLIDVTGLSLDYDEIRIPVGEWRSFPATVQPSNATIKGIILMSNNDNVVSFSGDGR
                     VKGISAGMAVVMARTLDGDFEATCTVKVYEPVIEGDIRFDSETGTIIQYTGVQDMVNI
                     PATIGGEAVQVIGENAFSNNRNITSVTIPSSVERIEYGAFQYCDNLYVIDIGTGVQIG
                     NNAVNNSNSFREAYTAGGTGKYVRQYDGSWNRVNQLISVTGISLDNNEMRISVGGEYR
                     PNRINATIQPSDATIKGIIWTSSNASVVSMMNDYGYIRANSAGTAVITAKTADGGFEA
                     TCTVTVYEPVIEGDFGIEPETGTIVQYTGTQNKLSIPETIGGVTVQVIGGNVFSYNKN
                     IVSLTIPSSVKIEENAFRWCNNLYVIEIGSGVQIANDAIGWNNYFREAYTAGGTSTYA
                     RQDNGRWNIINQVIDVTGITIENSEMRIPIGGDNWISIPATVQPTNATIKGITWTSSN
                     EAIVRVNGDGRIQGASTGTAVITAKTADGGFETICTVTVYEPVIEVDFGIDPETGTIV
                     QYTGTQNKLSIPETIGGVIVKVIGGNVFSNNRNITSVTIPSSVERIEYGAFRYCNNLN
                     VVEIGSSVQIADDAINNNNSFREAYLAGGAGMYARDGWTWNKINQFINVTGITIENSE
                     MRIPIGGDNWISIPATVQPTNATIKGITWTSSNEAIVRVNGDGRIQGVSAGTAVITAK
                     TADCGFEATCTVTVYEPVKEEDFEINAETGTIVRYRGTEDKVIIPETIGGIPVQVIGE
                     YAFYRNNYISSVTIPSNVRIEKFAFFNCNNLYVVEIGSGVHIAGDALYSSDSLHHAYI
                     AGGAGMYARDGWIWNKIDQLISVTGITIEKDAMRIPVVRDNWISIPATVQPSNATIKS
                     ITWTSSDESVVVLSGDGRMKGLSEGTVVMTARTVDGGFEAACTVKLYEPVIEGDFGID
                     PETGTIVLYTGTQDKVVIPETIGEITVKVIGENAFSNNRNIASVTIPSSVERIEHGAF
                     RFCNLYVMQIGSGVQIANDAINNNNSFREAYIAGGAGMYAREGWTWNKFDQLISVTGI
                     IIEKNEMRIPAEGESWISVPATIQPLNATIKGVTWTSSNENVVRVNSDGRIKGISTGT
                     AVITAKTVDGNYEAACTVTVYELIIEDDFVFDAETGTIVQYKGTQDKVIIPGVIGGIP
                     VQVIGENAFSQNSYIIGVTIPSSVVKIEYGAFLSCNNLYVVEIGSGVEIEDDAFCYEN
                     YFRDIYVPAGAGIYERSGPIWNKINQVISVTGVSFNKESIRIHVGEKYSDLTVSIQPE
                     NASVKGITWTSSNNAVASLEEGPIKGNSAGTAVISARTADGGFEASCTVTVYNLVLEG
                     DFAFDEETGTIVHYTGAEENVIIPSTIGGVPVQTIGKEAFYRNNHIASVTISPSVLRV
                     DEYAFKDCNNLCNIEIGSGVQILDYAVNWGNSFRDAYTAGGAGIYARQDHGTWVKINQ
                     TISVTGIFFDKNEIHIPFGENEYYRVKATIQPLDATIRGIIYSSSNEAVASISETGNM
                     KVNSAGTTVITARTTDGGFEAYCTVTVYELVVEGDFIFDPETGTITKYTGTQEKVTIP
                     STIGEITVQVIGEKAFYNNNHISSVTIHPSVQRIDEFAFSGCDNLYVVQIGSGVQIAS
                     SAIGYDNHFLDAYTVDGAGMYARQDNDTWNKIDQVISVTGVSFENNDMLITAGEFYDQ
                     LATIHPSDATNKGIIYTSSNDAVVSIHGLSIKSNFAGTAVITARTADGDFEASCSVTV
                     YYKTVTEEDFIFNAETGTITGFKGAHTTVEIPETIGEVPVRVIGENAFYNNNCLISVT
                     IPSSVERIEKNAFSFCNNLHIIEIGSDVQIGSNAMYNANYFRDAYTAGGAGKYTREKT
                     LPWYKIEQVTSVTGISLEKDEMRIPVGGKYWFYSNPATILPSNATIRGIIWTTSNKAV
                     VSLNPDAHIRGESVGTSIITAKTVDGSFEAFYTVTVYEPIFDGDFGFNAETGEIVQYR
                     GTSDKITIPSTINGISVQVIGESAFAHNQNGTSLTIPSSVVRIEKNAFSFSKILNVVE
                     IGSGVHIADNAFNNCNYFRDAYTAGGAGTYERIENTIAWRKVN"
     gene            complement(140101..140766)
                     /locus_tag="PLF14_00550"
     CDS             complement(140101..140766)
                     /locus_tag="PLF14_00550"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOT69822.1"
                     /translation="MRSYRFKLLAIVLVLLMIVTSVPSAALERDKAGNLVFADMPNNW
                     ATEALVNAVNNGLLNGYIEEGKQLIKPNGVLKRSEMLTIVTRAFGAEVIADLSSVVDI
                     PKGVWYEESIGKAVQMGITDLKGRMQPTRTVTREEVFTTLAKAFKLKTVGDDYTALDV
                     FKDKSRISKSMRSEMNAMVAAGYLAGYPDGTLKPKASITRAEFATIMNRLVRQYIYPG
                     SSY"
     gene            140988..142130
                     /locus_tag="PLF14_00555"
     CDS             140988..142130
                     /locus_tag="PLF14_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006197545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="three-Cys-motif partner protein TcmP"
                     /protein_id="HOT69823.1"
                     /translation="MMSNEFFQEQREQSIIKSRIVMKYFSAWAKIILAELKKYPHNDP
                     RMAYVDLFAGPGIYEDQNKSTPILVLEKILSDSDLCDRVITLFNDKDKANISSLEAVI
                     AQVPGIEKLKYAPCFYNHEVGNEIAEMFSHRKIVPTLFFVDPWGYKGLSLDLISSIIK
                     NWGCDCIFFFNYNRVNMGVPNEAVRQHMISLFGKERFNLLLANLHDVLTSDEREMIIV
                     QGLCDALRENGSKFVLPFRFKNDNGTRTSHHLIFLSKDFRAYDIMKEIMAGESSDSKN
                     GVANFEYNPRDKFYKQGTLFDLLSSPLDDLKDGLMEQYNNQTIDFLELYREHSVNRPF
                     IKKNYKAVLRKMYDEGLISAVNRKTDKPPQKGTFSDEMRITFRSNK"
     gene            142127..142876
                     /locus_tag="PLF14_00560"
     CDS             142127..142876
                     /locus_tag="PLF14_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage Gp37/Gp68 family protein"
                     /protein_id="HOT69824.1"
                     /translation="MRTTKIEWTDRTWNPVTGCTKFSAGCAHCYAETMANRLRAMGIK
                     KYANGFLPTLHEDSLYEPIKWKKPHTIFVCSMADLFHEKVTFDFIDRVMATIRQTPHH
                     RYQILTKRAERMAEYFSQTDIPKNVWLGVTVESSSVKHRIELLRPLQATVRFLSCEPL
                     LEDIGEVNLTDIDWIIVGGESGVKARPMKPEWVRSILQQANQQGVAFFFKQWGTWGSD
                     GVKRNKKANGKTFDGKIIQMMPHFKECINLE"
     gene            143294..143506
                     /locus_tag="PLF14_00565"
                     /pseudo
     CDS             143294..143506
                     /locus_tag="PLF14_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588982.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
     gene            complement(143512..144285)
                     /locus_tag="PLF14_00570"
     CDS             complement(143512..144285)
                     /locus_tag="PLF14_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT69825.1"
                     /translation="MDSKKALRIIIVLLLAVNIFMAAFIINLVYSEPDTKDEHKYITE
                     ILAYRDITLDCEIPQYAAPSAVIVKSDSDNSALLSYLEAQGGTISDSQEDVIIYTPAV
                     KQRYEDLTLETAAKIADDFIKKLPVDSQSYMPDSILTAGVGVYKVNYIYLDGSYCIYD
                     KKIEMTISKNGIDRVYIGCPEYTVAETEYNRPMVSVGNILVSGFTRQGRSFITIKKID
                     AGYIYDKNTSSFVNVWRVMYNNGQERYFSSVDGTVIVAD"
     gene            complement(144285..145712)
                     /locus_tag="PLF14_00575"
     CDS             complement(144285..145712)
                     /locus_tag="PLF14_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT69826.1"
                     /translation="MRKSIAYPLLIILILLTVFQSFMLINYHSTGRLLPKKVIPGSNT
                     VTDVMNFEEYLLETYIQPSEITMTSGEGLNVHKITKQSSAYYTQIWKDTVTLLDTISE
                     LKVYEIYDADKWNEMTGKSGYIVKFGYDCPIEFINRISDSVNTNNEIKSIDKIMILPE
                     STDEGDVYINSGEKVYRYIGVKLNGLLKGNEFLSIYEEIKQKSDITYVYLNEVVSYDN
                     FKGNIQPDTTVVIMDDSVAIGSIRVAGYELLTECMLNLQIEPDVNNLSPQVTRYISEI
                     KTKLFGVYSDIYRVIIAQNRDLTFSDQYNIYTIHTDGSVTYRYASASQIQERGEIALA
                     FNNALSMLADLTSLSATEKNPPVLAFVGIEEDCYEFRFVYRYNGYPVLLDKEKYCISI
                     TATADRVVAADANLFDISDASLPTVMYDLRTFNMLSENDMALGSIKANGMYIAYMKDD
                     STVLLPKWIIEGEEITVLGLKEVQD"
     gene            complement(145709..147454)
                     /locus_tag="PLF14_00580"
     CDS             complement(145709..147454)
                     /locus_tag="PLF14_00580"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT69827.1"
                     /translation="MVFYICIIAVCMIIPVGVLMNNNVEQYHYTQFTGNIENGRKMLN
                     YTQGEPLNIYTVYTDMRDLYASSFGIYGDNRSFTVVDRNTNEIYSNDSVFLSHDSSLF
                     INELFLSYNFLSSMAGTPLNSKALQNIRGRTFYDYAITLENLVIYFRYYKTDWQAMVM
                     QFNETLLTALLFSIAVAFVVGYLLSRAISRPIKDMTVRTKAIAEGDFGRTLDVKADDE
                     IGQLSTSINNMSYSLKNMLDEISSEKNKMEIILDNIEGGVIAFNLDGQMIHNNPAAYT
                     LLNRPDMEFTLESFVKEYNLDIDVSDIPADFYKEDILPVDEEMLAFSIASFKDSLGNA
                     EGVILLFRNITEQYRLDRMRKEFVANVSHELKTPLTSIKSFTEALMEGVEDPATEQHF
                     LEVINSESDRMDNLVKDLLQLSSMDMGRMILKISHHNVTSLITDALQAVEAEAKQKNH
                     TLISDIAFKGIAYFDYDRMLQCLINLLVNAIKYTDESGRIEIKAYEESHKLCIAVKDN
                     GIGISKKDQERLFERFYRVDKARTRIMGGTGLGLAIASEIVKAHDGDIKVESKVNQGT
                     TITVSIPLGKKEAAQ"
     gene            complement(147480..148166)
                     /locus_tag="PLF14_00585"
     CDS             complement(147480..148166)
                     /locus_tag="PLF14_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT69828.1"
                     /translation="MGHKILVVDDEKNIVDILKYNLEKEGYDVICAYDGKEAIFQALK
                     HKPDLILLDIMLPEYDGFVVCRKLRESTTVPIIMLSAKSEELDKVIGLEMGADDYMTK
                     PFSARELIARVKSNLRRVSYANVDNSELLSFKIRDLEVDEGKCEVLKNGQVLELTARE
                     FELLVFFMRNPEKVFSREFLLDKIWDYEYYGEMRTVDVTVSRLREKLSGDPNEYIVTK
                     RGMGYYFKKD"
     gene            complement(148214..150937)
                     /locus_tag="PLF14_00590"
     CDS             complement(148214..150937)
                     /locus_tag="PLF14_00590"
                     /inference="COORDINATES: protein motif:HMM:NF020730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT69829.1"
                     /translation="MKRALGIILIAVVLLSYCVVFPKAYTSVEIAYGLNILRGTGSGL
                     DEAYLSSVPTRSNAAMIILRLLGLEDEALECEGTATFRDASSAAPYWQPILAYLYANP
                     SVGFSGFGDGTFNPNGSINAQMLAKVLLTVLGYRQDVDFSWNSTLLFAESVGLRSLKN
                     KTGITNTDVAVALVEALNIRTKEGYTLVTELVAADVISLERAEKYGFVVESTEVMISG
                     ASVTGVRSITVQFFMPVSADTSVVLRKDLIGIANTYEFSANRKKLVITTSATLSPGTY
                     TVVVGGKVQNLTVEAEKAQSLIITGDRLYKTFGQDIGLKLLNQYGESMSLANVNVSAT
                     NKTSGTRKAVISKTETSVLIDAALTNIGDQIYIYALDNKTLLSDSVQLTVQSVPAIKR
                     ISVTEVTEASGKERIFEDSSMHVIKLSAYDQYGQVYKVRQADIDSGSLMLISSNSLSV
                     SVSSIRVNSEGNLLFNAGKAGDTRLHIIVKEEGISTSADIQVSQAPFLSSIKAEQLPS
                     TIFKNERIEVNVMGYDQYGNIYEMTAADAVSYTSSDTAVVPTANIQISNGVLSFNTLS
                     SGVATVNYSIKGGIQPLLTVNVNDGNVPYIVTGMEMPFTHFEKGVKNVVLDLDDVVVV
                     DQYGNPNSLSSRPSWDTVEWGVYIDKVSGNSFAYEGNIFTTTDIPGTDIFNIYITRDG
                     RIMDRSGYSFSLTNVDSENIQIFDIEVPEVIYGGANNRIDAHTKYISIRGYTLSNDRV
                     MLKTDESGLPTVISSVTVTGNKVYVDTATWAIKFHSYYTENDIVTVKFWKESKEVKSA
                     DIVVLAAESKVSRIEYDANQSFVISSREFYTEPLRIYDQYDIEANLPSNTRWLCNSNF
                     VDSITFDPVAKRLKIVVRGTVTTETEAIISYIAPDGSFAFRGFYTIMPGTY"
BASE COUNT        42171 a        33786 c        26985 g        48058 t
ORIGIN      
        1 agaccgtata tctgcatttt actcaccgct atgacactgc ttttattaac ataaaacgga
       61 cctctgcctt cggttcccga gaccagataa tattcatcct ccacactttc cagatgtctg
      121 accgaatatt gtattcctgc catgctcttg gtggttatta tcacataatc acccttctcc
      181 accttatcat atttcagatc ggacaacaaa gcgctgcctt ttagaatctt aggggaaagc
      241 tcagaatcat tgacgctaac caatatatgg ttaccgaatt tgggacatgc atattgcata
      301 tcaaagctta aaatcataat tgtagttatt attgcggaag caatgaaaat taccgatatg
      361 aatgtgtata aaattttgaa aattttcttt tttttcatca attaatctcc ttatgcggga
      421 tctacaagtg tgaactccag ttcaaatgca aatccccctc ccgcatgtgc tcttaccgca
      481 taagcatttt cgccttcaaa ccaaattctt atagtaagtt ctcttacgct gattgtaccg
      541 ctattcgcac atatgagttt aaacggcaat gctcccgcct gcccgctctc atatattccc
      601 atagcgattg acggatcgct cccgcttatt gtatttatac cggttaccgg caatgcagca
      661 ccggaggtat cggtaccgtc tgcggcatat ctgtatatat atgttgtttc aacaccaagg
      721 tcatccgtca ccgaaagtga tatccttgcc gctttataaa gttcattgtc ggtaagaccg
      781 gatattgcaa ctttgcttac aaaacagtca agtgccgtta tatcactatg tgcttctgtc
      841 gtagtaacca aataaacggt tttttccatg caaaatttct tatcattctg tccgacagcg
      901 gcatattttg taccggtttc cggatcagtt atggctgaac cgtcggtctg tactttcctg
      961 gcaaaccaga aattttttcc gtcgaaagag gaagaaggtc ttaatacgat attaactccg
     1021 taatcatata agcttgagaa acccaattcc tcaaagtttg ccatggagaa agtaagttgc
     1081 tgagacagtc tttcatcctg actgccggat atgtatacgg tcaaaggcac catcgcactt
     1141 aactcaacgc cgtttgcttg tactttttca ctttgagcga accatgcatc ggaaaaccac
     1201 caataggaaa cgatggccac ggctacaagc cctaacgcca gtctgattcc cgctgaaagc
     1261 aatttccttt tcattattcc tccatctttg caaaaacaat agcagccttt gcaaataata
     1321 taattgcaac tggctgccta ttacttttca tacggcccta tagttttgcg tcacaccctt
     1381 tcgggtattt tgcctttctt gtagaataat attatcacag tatgtaaaaa ttgtaaataa
     1441 gaacttttgc agtttttgcg gttttttttc actttcttaa aaatatgaag ccgaaaacaa
     1501 aaaaaccgct aaaaatccca tcagaaaaag ggaaaaatat gctttaccgg tgttcagcaa
     1561 ttacccggat acacatattt aagatatttt ttcgcctctt ctacaaggtc gtataccgat
     1621 tttcgggatg taggtctttt atcggaatac ttatatctcg gataaaatgc ggagatatga
     1681 tacggaatat tttcatcaat acttgcaatc catctgcttg cactcgttat ctcttccacg
     1741 gaatcattct caccgggtat aactaaagtg gtaacctcaa catgacaact ctttgaggct
     1801 gtgacaattg tgtcttttac cgtttccaaa tcaccgccta tcttgcggta aaaatcctca
     1861 ttgaacgcct taagatctat attcattgca tctatataaa gaagaagatt atctaaaggc
     1921 tccctgcaga catatccgtt ggttaccagc acattcttca gccctttctc acgggctaaa
     1981 acggaacaat ctttaacata ctcataggat atcaaaggtt cgttataggt ataggcaatc
     2041 cctatattat tcctgtcttt cattgataaa gccacatcca ccagataatc gggctgcgtc
     2101 tgatatgttc ctaccttgct gtcgtccgcc attgatatat cataattctg acagaacgga
     2161 catctgagat tgcatccgta acttcccacc gacagtatca tctttccggg aaagaatctg
     2221 tataaaggtt ttttttcaat cgggtccagt gctatggctg tgagtttgcc gtagttttcg
     2281 catattatct ttccatcctt attgatcctg gcacggcata acccgacctg cccttccgaa
     2341 agcatgcaac tgttcggaca tatgggacat tttatcttca tttgtgcctt actacctcaa
     2401 atctctccat ttcaaatttc tcaccgctgg ctatacctgc cttactcagc gctattgcca
     2461 gttgttgctg cacggtatcc actccttcca ggttgggaag cagtaatcct cttcggtagc
     2521 ccgatgtaac gatgactccg tacctgttta cgtccagttg gtctatgctg tcaatcctct
     2581 ccgcctcttt cagaacatcg acactatatt ccagtgcgtc caattcctct tcacgcaccg
     2641 gaaagaatct gggatcatga gaacaggaac tgaccgcatt ccttattatc tcttccgcaa
     2701 tgttttcggt aacgggcgaa attgtcccta tgcatcctct caaatcgccg tcttttttca
     2761 gtgttacgaa cacaccggca cgttttgctg tcagttcggc cggcaggtcg ggcggcattt
     2821 tgagcatccg tttattcttc acataatatt ccacggaagc ttttgccaaa gccacataca
     2881 tatccaatct aacttctttt tccttttgtt caacaagaaa agatgctacc gcatatccta
     2941 caccgaaagt gttttcgtaa gataagagtt taggttctat tgttttttta tccagtgcac
     3001 cggccataat tacaaaggta cgatgtccgc actcggcggc tttttccaga aactcctccg
     3061 agaaatccat gaatttcgcg aaatttccct ttgccatggc ttcggtgacc atctgatcat
     3121 attgttctcc ctgctttgaa tatccgtacg gtccagtcac tttcagttta tgcgataaat
     3181 caccgcttgc tatgaaaaca acacgcctcc ccagcaattc cgccgccttt gcaacacaca
     3241 tacccaactt gtagtggatt tcaaaaggca gaagggatat tcccatcctt ataagcttat
     3301 aatctctgta tacttcattc agaaaaacca acggaattac gcttgcatgg tccattccgg
     3361 ggatttcccg accttgggta cctgccggta tattctgttc ggaaataatt ttaagaagtt
     3421 tatctctgaa ctgcttatca tattccgtcc gcaccgatat agatgcggca ccgaatcttc
     3481 ccatatccga ttcaaagtat tcaccgtccg caatatgtaa atagtcggaa tacatggcac
     3541 aatgcgagga tgccagaaca atagtatcgg gttgcaactg tgctatggtt ttacaggctt
     3601 ctttatatga gtctatggtc ttctgtatct tcttttcttc accttttcct atcttcggaa
     3661 gtataagcgg aggatgcgga aaaatgaatg ctgctgctac tgacacaata cacctcctat
     3721 tttgctttga ttatgaaaaa attataccgt aaaacggtgt tttattcaac aagccgtcac
     3781 cgttttgcta tccgtttaac tatcttgctt aacagcagtg aaaagttccg tataaacatg
     3841 gtataatact ctacgacgtg ttgttcacgg atatggagga cttaatgaaa aagataagat
     3901 taccgattat attacttata ctgtcagtga tgatttcctt tgctttctca ggctgtaaca
     3961 gcaaaaacca ggatttcact ttcagcagag atctcgataa taacggttac tggcgcggtg
     4021 taaaggcttt gaaatatgtg gagttgtgtg attataaagg tattacggta cccaaggaca
     4081 tacatacggt atcggatgac gaattacagt ctgccattga aagtttgctt tccgaacagg
     4141 catcttctat aaaagtaacc gacagagcgg ttgtttacgg agacacactc aatatagact
     4201 atgttggcag aatcgacgga gtagcttttg aaggcggttc caccggaggt gcaggtaccg
     4261 aggtgacaat aggcgttaca agctacattg atgattttct tgagcaactt gtaggacata
     4321 gccccggaga aactttcgat attgaggtaa cttttcccga taattacggg aaggaagaac
     4381 ttaacggaaa agatgcggta tttaccgtaa caattaacca catagtggaa tcggtaattc
     4441 cggagcttac cgatgctttt ataaattcaa cattcagcca gacaaaggga tggacaaccg
     4501 tcgaacaaat gaaggcggat gtgtataagg atatacaaag tgccaaaata gccgactata
     4561 ttcaggaata tcttcatgcc aacagcacgg taaaggagat accgaaaata ataaccgatt
     4621 atcaggacac tgccgttatt aaatactatc aggactatgc ggactattac caggttacac
     4681 tggaggagtt tttacagcag tacctgaatg tggaaaatca gcaggcactt ttagaacagt
     4741 ataaagagga taatataaat gtatccaaga tgcaccttat aacccaggcg attgcggaaa
     4801 aggaaaagat aaaggcaaca gccgatgatg tcacccggta ttttatagat tacatgaata
     4861 cggatgacta ttcgcagtat gaagaagtat acgggttaaa ctatcttaaa atgactgtat
     4921 tggtacagat ggtgattgac tatgttcaag cttcggttgt tctggaataa ataaattatt
     4981 cattcagttt acgtataacc ctacagggat tacctactgc cagacagttg gcgggtatat
     5041 cctttgtaac cacacttccg gcacctatta cactgttatc tcctatggta actccgggca
     5101 gtatatgagc accggtactt atccatacat tatcgcctat tgttaccgga tatgtgtatt
     5161 ccagtccctg tatgcgctct tcgacaatca ttgaatgtac ttcggttatt atacagacat
     5221 cgggggcaat gaaaacatta tttcctattg ttatcttacc gctgtccatc agcctgcaat
     5281 tgacattaag gaagaaatta tcacccaccg tcgtgttgta tccgtaagtg cagaaaagag
     5341 gttgttccac tacgcaattc ttccctgttt tacccaatat tccctttatc gcttcttttt
     5401 tcgcttctct gtctaacgga tgtattctgt tatattcata caacttctgt accgttacat
     5461 ctctgtccgc taccagttcc ggatctcccg gttgatagag catacctgca tgtgattttt
     5521 ctttttccgt catgattttc tccctgttaa atattcatta ctcaatactt gcaaaacctg
     5581 atatattaaa cctcataatt gttcctctgc cgacaaattg aataaacccc acaccctcaa
     5641 gtgaatatgt gccgactgcc gtctcactga aatgctttgc aataagattg ccttcggaat
     5701 catatagctt tattgtcgta ggctcaagag cgccaaaaaa gaatcggaaa ttaccgagct
     5761 tatccccttt atactcggtt atataccttc tcaactggtc tatattaaaa tatatatcct
     5821 gatactcaaa cccttcaaaa cacattgcac ttaacctgta gttatacttc ggatatatat
     5881 tgatatagga aagatgtgtc ctgtcagtag tattatcagg tttttcctca aaaaagcctt
     5941 catttcgctt caaccaggta taaaaattag cttccacata ctctccgtat gtcataggca
     6001 gtacacgatt aatctccctt atatccaatc tgtcatccga aacatccggt gtgaacggat
     6061 ttactttctg atattcggtt atccaggacg agtaatcgtt gtaaacactg tcaagatatt
     6121 tcataaggcg aaaacctatt gcgtaatcgg aactcattga atgcggtaaa tggttctcct
     6181 gccagtaaga aagcggtttg gaatacagct ctttatatcc ttctgcactt attttctcat
     6241 ttctcatatt attttccgcc gcttcaagtg cgtatgctat atcgggataa ttctcttcca
     6301 tatattttgt cactttatat gtcgtatata ccgcaaaccc ttccggcagt atccttcgga
     6361 aattccaacc gcaattatga ttctgaagta tatgactcag ttcatgagct atggcatgtc
     6421 ctccggaaat aaacaaatca ccggtactga taactatacg attgaaatat gcatatgcta
     6481 tatgggtttc cgacacatac ccgtccaccg cttgggcttt ttttacttca acaagaattt
     6541 tgcccagttc tgccgtgtta aaggacaatc ccgacacaac ttccagtgca tcaaacaggg
     6601 tttccagata atattccaga ttatctataa tataagtatc cgcatcaatt tcacatataa
     6661 agtgttctgt ttcaatagtt gtatgctctt tagtaatgta tcccgaagga aacggcggag
     6721 gggtaacggt ttcaaaatca cacttactgc acttttttgt ttccgggtcc atatcgtgtt
     6781 tttcagtgaa tctgttaccg catttcagac aatatccgta atggttttca atatcaaaat
     6841 catattcttc actgctgtgt ccttccgcag gtatcacttg ctgtttcacc aaaacggtat
     6901 tacacaaaga gcaatgactt ccttcggtca gaccggtttc ggtacaggtt gcgggcaccg
     6961 ctttatctat aacaacttca tgggaacaat attcggtcac ttgcggtact ttatcgcagg
     7021 aagacaagca gataaccatg aagaaaagta atattattga tatgatttta gtggatttcg
     7081 gttgcacctc ttgctctcct cttctaaaaa tccgtttcaa acaatatagt atcatattta
     7141 tatcagtatt ccgataaaaa tagtcaacag aaaaccgcct tcactatggg aggcggcttt
     7201 aagtattact ttattcttaa ttcattttat ccagcatacg cattaatacc gttgccattt
     7261 ccgctcttgt cgtgtactcc agcggattaa aggttgtctc ggttcttcct atcataatct
     7321 ttgttcccac aacatacttg gccggttcga ttgcatattg agaaatactg ttgtaatcgt
     7381 catatgaggc tatattcgtc tcctcaacaa ccgaggtatc cagtcctttg tattttgcat
     7441 aacggtaaat catggtcgcc atctgttcac gggttatgtt gttgttggga gcaaaactgt
     7501 cttcggacac accgtttaca atgccgtttg cagctgccca tgcaaccgca ttttcgtaat
     7561 aactgccctt gggcacatcg gtaaacgggc aaggttgcga ttcaggttca ctttccatac
     7621 ggtgtataac cgttacaaac attgctcttg tggcaagaag ctcgggagcg aattctttat
     7681 cggaaatacc tctcatcaaa cctgtttcgt atacgaactt aacagcttca tagaaccagt
     7741 cattctcgtc aacatccgta aactgatcat accattgtgt ctcttcattc ttagtccact
     7801 ttgcataaag tgtaaaggca cttgtaacct ccttggcaaa gttataacgt gtcttcaagg
     7861 ttgtatctgt ataccagcct tcaaatgtat atccggcttt tacgggattt gtcggttcgg
     7921 caactgtttt tccgtaatct attcgttggc ttctgacatt gcttccgccg ttgggattaa
     7981 aggaaatgta gtaataaggc gtactgttgg ctgaaatctc cgctttgacc tttgcaagag
     8041 cattggaaag agctgtggag gcgttgtcta tatcactctg atgtatgcct tccgtcacaa
     8101 taatctgttc tgcgctgttt atagcattct caagttcatt ccgggtgtta tccgttaagc
     8161 cgtctttctt aatctgtttt gcttccgcaa ggagttcctg taagcctgtg aaaaccaatg
     8221 cttcatccga cacttccaca acaataccgt cataaagagc atcgtttccg cccaatgcga
     8281 acttggcata tatgagcgat tgccctacaa tacccgcagt gacggttgct ctgtccattg
     8341 ataccgccgt tccgctcatt actttcaacg taattcccgg aagattggta atatcaatct
     8401 tctcattgtc cgtctttccg tacactttga gttgcgcagt ttcaccctct gcaagcgtaa
     8461 gcttaccttt tattaccggt ttgtcaggac attcaatact gattgacttt atttccgaaa
     8521 gttttctgtt caacagagtc agagtcttaa acacttcatt tccggcggca tctcttccgg
     8581 taatatccag ttgaattctg gaaagattct tagcttcacc gagaggtaac gtacatttca
     8641 tcagtctgtc ggtaaaaatc ttatcctcct ccggtgcaac aaccttaccg tttatcttga
     8701 aggtatatgt cgcatcctcg tccgctgtcg ctactaccgt tacactgtca ccgtcaaaga
     8761 aactgccgtt ttgaggtaag gcaagtaaga taacgggagg tgtggtatca atgtttatta
     8821 tttccgtggt aatggacttg tcgccgtctg catcggttgc gtggaattct attgtatgct
     8881 ctccgtcggg aagatttacg ctttgcgaaa tttccgtaga catttcttta tactcatacc
     8941 attctccgtt ttgtgtagca gcgttaatgt acatttcgcc tactaccggt acatcggatg
     9001 ttaccttaat ctctcctgtc gccttgtcgt atccgatagt aataacgggc ggtgtttctc
     9061 cttttaacac cactgaaccg gttgttgcat atgcactgca gtgatataag tcgttgccgt
     9121 ccgcatcctt ctcaatattg cacaatcgga ctttagccac ataggatttg cccggagtat
     9181 aacctaccgg aacggtttgt attaaaggat ttccgtattt atcattaccg cctatcggaa
     9241 tcgggttgcc gttttcatcc agaacaggca tcatatagca tcctccgaca attatttcct
     9301 cgcctttggc aaaatacagt cccgtgtcgg caagcagtcc gtcttcgtaa acttcgacaa
     9361 gatatccgtc aaagttatcc tgtgcggttg caacatttat tcttaactta tcgttaccgc
     9421 agttttccat tgttgcactg tcaggggcac cgggtgcctt gctgtttgtt attgtaactt
     9481 ttccgacatc atatgtatca tatgaaacat tttcttcgct caatgcaata acaatgttgt
     9541 actccccgct ggacattttt tccggtatcg tgattgcggc actgtactcg ttagctttta
     9601 ttactctcgg gttgagtata atgctgttat ctttattacc gtcatgagcg gatataacaa
     9661 cggttgcagt atcgcttata ccttctccgt tccatgtaac attcaaaaca ttcccgttaa
     9721 ccgtagcgtt ataagttgta agctttgcta tcgggtttac tttcagagcg ctgacatcgt
     9781 aagggttgtt gtcggaaaat tctatcagga agttttccgt agcatctgcc ttaggtataa
     9841 cgatatttgc cgcttttccg gaaacattga cattggcatt tttgagagct gccgctttta
     9901 catcatccga agcattgtga ggaggagcga tatagtaact taaaggatat acggcattgc
     9961 cctttttaac tgtcatacac gcctgtagat cctgtaccgt taaatccgaa ccgtctacgc
    10021 ggctgacagt gaggatatag tcgcaggctt caccgaattt gataaggtga ctggttcttt
    10081 cggtgttagt gtatatttcg gtatttaatg cttgcggacc tcttaagttt ctacgcatac
    10141 ctgtgtttct caaattgttt atattattga aagcggctaa tctttgttca aaatcgtcca
    10201 cagccgtgct tccggccgca tatgaaaggt tggttcctac agctgcatat tgtacttcac
    10261 cggtcttcgg atcctttccg acttcattgg gtgaaatcat acggttaaac atcatcttgg
    10321 ttcttcttac accgggatca tcggttgaag acattgctgc gaagttctca cctccgtcta
    10381 tatcaccggc tgtaaattcc acctttcctc cccaataata tatgaatcct acttttataa
    10441 gctgcaacag ctcaattgag ccccatacct tctcgctgcc gcctccgagt tccgccgatg
    10501 ccagttccat accgcctaca atgggaatcc atttgggcag tcctatggaa acccttaaga
    10561 caaattccac aaaatcgtca atattcttac ctgccgcagc ggatattgtt aatcttccgg
    10621 tcattatctg ttggaaagtt accgcaccgg taaagttaaa gaagaagttg gggtaccagc
    10681 ctatggcaat ggatccgccg tcaataaact tggcatcctt aatcttcttg atactcaagt
    10741 cgtcaaaggt tattttgata tagcgcaggg aaagctccaa atccgcacta cccgttaata
    10801 tcttggatat atcaaactga atgttgagta aaattgtaag aggcggtata ccgtctttac
    10861 cgtaaatggt atcatacaga ttgtctattc cgcctccggc tcccgtcagc cagaaaacag
    10921 tcaatccgtc tatattatag cccggctcaa atcctccgac cgaaaggaac agcttgtcgg
    10981 ggataggtgc tccgttaggt gccgatataa taacaagtgc gaactctatc tcaacattaa
    11041 atgctttaac ctccgcatca atacctactt tataaccgtt tacggtatta atagctaagt
    11101 ttgcacctat ttttttgggt aagaaagcca caatctgcgg caatgaaaag tatgcctcca
    11161 tgttaatacc gagatatccc ggctttttgc ctccgaagag tacatcatac acatgtacac
    11221 ctgcggaaat acgttttgct tcctttgttt caggctcaat atcttcttca taagtctggg
    11281 tctttatctc accggttgca tcatcgaagt ccagtccgaa tgaatacata tcgtcatcat
    11341 ctttatattc cacttccgaa ccctttgttt tccagttttt agcggcattt ctgttctctc
    11401 ttgcaacctt ggcaccgccc ggtgtaataa atccgagatc aaggcctccg ccgaaagata
    11461 taacattggt tattgtcccg tcctcatatt gcattcttcc taaaacaccg tatttcagat
    11521 cgataaggaa tccgccgatt gtggttaaaa catccatggc attatcccat ttcagctcaa
    11581 taaagtcctg cccgactgca agtgatccgt cctcctgcac tatacctcgt tcgtcataca
    11641 gaggaacaag gtactcgaca ccggccttaa gccgaaatac cgctgtaccg tcccgcacca
    11701 tagtgtttgc acccatggtt cttatttttc cgtccatcag cacttcaacg gttccgttat
    11761 ctttctgttc aatagtcaaa tcgcttccat ggtaccaaag tgcatagttt atggttatat
    11821 tctgcccctg accggataaa cgataaagat ttttattgga ctgttcctgc agtagggggc
    11881 ccttgaatgt aagcaacaaa tcattaaacg gaatctgagc atctcttaat gcatcttcgt
    11941 ctttatacgg tacaacttta tacttattat ttcccgctcg ctgtacggta acaataccgt
    12001 agaaatcatt cttatacttg agatcacccg acatctgaac atacattgca tccgatgtaa
    12061 agtcggaagg tacaccttgc agcacgcctt ggcctgtttt atcgtctttc cacttaaaat
    12121 gcaactgata tcttcccatg tacatatgtc cggtaagcat tatacttatt ttctctggat
    12181 cggcttggtc aaaaatgata ttttggggcg agacttcata gtttctgccg ttgtcgccta
    12241 caaaaacaat tttttccaac aaatcacttc tgaagaagga cataccttgt ccagtaacag
    12301 taatatacct tgtctcctga ttgtaaacga tatcgggagc cagtccggcc agtgtaatgg
    12361 tgggaagggt ggaatccctg cccggtatta tgatatggtt ctcggttgtg ccgagacaat
    12421 aatcctcagc ataggagccc agctcgttga catcctcgtc aatgtcgaag accttgataa
    12481 tgaaggtacc taactgtgcc cgctcttgcg gtgtaaactt aaaatcaaaa tatgttacgg
    12541 tattatcgcc gcttttgaag tcggtgatat atgaatacat cggactgata ttgctatatt
    12601 caatccagga gccgccggtg gttatacgca gcggggtaaa acctatcgca tatacaacaa
    12661 ccgcaagttc tttatagtct ttttccgcat acagaggatt ggttacattt acatttacgc
    12721 ggacaatatc atccacattt cccgtaccga cataagctgt acgtgtttca tccgtaaact
    12781 ccaactttga cggggccgtt gtattgataa taatcttgtt atccttattc ttcaggttag
    12841 cggtaacacc gtagtaggtg gagaatgttt tggatttacc cggttcaata acacccaggt
    12901 catagtacaa tgcggcggca ctgtccgcag tctttttctt attggcatca ttggtaaagt
    12961 tcagtgttgt aacaggctca tagtcaaata ccgtttgtgc aatatttgcc cagtgtgcaa
    13021 aggtcatttt atacggtttt tgctccgtga aaacactgtt tacaccatat gctacgatag
    13081 tcgaggaata gggattatct cttacaaaat agtcggccgg cattttgatt gtcgggtccg
    13141 ccttggagtc ccaggttctt tcaaactcga aatactcata gccctgaccg agatgctgat
    13201 tgggtatttc atagtagccg taatcgtttg agcccaattg tgtgtctata agaacacggc
    13261 tctgtacgct cttggcagcg gctgattcgt ttttaacatt atagctaatc attgcagttc
    13321 cgagctgctc cgatgcctca ctgttaataa gtgtaataac ctgctcgaat acaaagtccc
    13381 cgatgctcca ggtgcttttt ataaaagcac cgtttacatc tttttcggta acggcatagt
    13441 tactttgcaa gcctaaaaaa ccgtactttt ctccgaacac atattcatcg ttatctactt
    13501 taaacgaagt gaatgaagta tcataatatt cgcctctgtg agtaagaaaa gcattgttat
    13561 cgcttttttt caggatatcc ccctccagcg tggaaattac atatcctccg tttttattgt
    13621 tcacaataac cttaatatac tggttggata tttccgttaa gtcatcatcc gcggcggaaa
    13681 caattccggc aggaattaga gcaagtataa cggaaaggac caaaattatg gatataaaac
    13741 gtttaaacat gttactttat acctccgttt ctaatatata aataaataaa tcagatacat
    13801 atttctatta acatctttag ccataatagt gtagtatccg tcttgcgttg cgtagaagcc
    13861 aatttccggc ttaaatgctt ttgcgtattt catctccgcc aaggtcttat acccggcaga
    13921 ataatagtac tttgttgttt catccgtatc gtctacggta tgtttacctt ttgctccggt
    13981 gaccgtgtca aatgcataga ttttcatgcc gtccaagtta atggtaaaca ggttttccac
    14041 aacactgacc gtgcaattct tattggtaac attgcccagt ctgtcggtag ctgtaatggt
    14101 tgcgatatat ttatttgcaa cattgaaatt cacattacta aaatcaactg ccagcgttac
    14161 accgtcattt acattttgca tcttatcact taaaggagca ttggctcccg actgtgtatc
    14221 aaaagcggtt gtcagttcca aaaggctttc tttagcatct tccgcggagg ttcccgctaa
    14281 gacaattaca ttattaccta ctacccgcac ggtaggacct tctctgtcta taaccgaaac
    14341 atttgtccag aagacgtttt tattgcccca tttgtcggtt gcggtaactt cataggagcc
    14401 gttctcggta acaacaaagt tgtagttata gtctttttcg tttgccgtaa cagcttcttt
    14461 attaacagca agtgaagcgc ctaatcttgt ttttgccgtt acaacaagtc cttcgttatt
    14521 agttccctca tcggtaacat taagcaaaat acgtacatcc ttagatgtat acacatttcc
    14581 ggccttgtaa ctgtctttag gtgaaagcac agctccctcg ccgacaaaat ccagacttac
    14641 cttaacattg ggagcgagat cgtcaatttg cgatattacc agatggtcgg tatatacgtt
    14701 acccgctctg tcggcaaagt tcagtgtctg cggaccgttt tcctcgaatg taatagtgtg
    14761 ttggtttttg tatccgttct catggttctg caacaacatt accggctcgc cgtttgcaaa
    14821 aacgtactta atctcttctt tattatcaat tatattacgt tcaggataac cggctgcagg
    14881 tttgggagtt tctttgtcaa ttgaggcaat aggtccgtca tccggacgcc atagtgtcag
    14941 cccgttacct cttaagtcat agattaccag tttagcacgg caattctgct tgaattccac
    15001 aaccaccgta ggtccgcttg taacagctgt aacataatca tctgtaggtt gaagctcatt
    15061 ggtagtttca tcattttcgt aagccgttac ttctgtattc ttcagcattt cattacatgt
    15121 aatgaacact tttatggaac tgtttgtggg tgtttcaaga agttgtctca gaagttcttt
    15181 atcgcttgca tttgcatcaa agatgctctg ccaaccctcc agtacaactt tcggcgcttc
    15241 ggtatcaatg gtcaaaacgg caataagaac atttcctaca tttccggctt tgtccatgag
    15301 aacaaactta cctgtaccgt tcatatccag ttcgacatag tatccgtcga catcctgctt
    15361 cagttcaaac ggtgttccgt cggtctttgc gcccgttaca cttataccgt cgtctctaat
    15421 atcggtatcc tgtgtgacaa gatatgcctt aacattgaga ccgtccgctt tgtaataaac
    15481 ggtgccggaa ggagcggtta agtccattgt atttgcacaa agcttaaccg gaacggtaaa
    15541 atcattatcc gtgcccgtac ccgtaagggt taagttaaca actctgtcct catcactttc
    15601 cgtagtgaac atgacaagtt tttgcttgtt atataccgat acaccttgcg gtaagccgtc
    15661 atttgcggta atgatagatg aaacaacatc accggctgcg ttaagtgtct ctattcttac
    15721 cgatactgcc atgttaacca aaccgaggcc ggttatttct tcaccttttt tgaactgtcc
    15781 caaggtactg aaaccgttat cggaaggtac ggtatatgtc agacggtaac ctgctatttt
    15841 cttatcggtc ctttctgtaa atactatatt tgaagcatcc accgtatatg aaccttcgtt
    15901 tcccgctgca tcacggaaac ggaatgtgta tgttctgttc accgaatttg catcaaaagt
    15961 gacatatgaa ggtgccgatc cgtccgcatt ggctatcaag gttacatcat cttccgagta
    16021 gcctaaaatc acatatgtcg cggaataaac aagtgaagca ccggtttgaa tatccgtttc
    16081 tgtttccaat tccagtgata cgcttgcatc gggagccgtt ctgtcaatat tcactatcgg
    16141 aatactgaat gtttcagtct gccctaattc cgtattggta agagaatagg tcaataaacc
    16201 gttcgtaaca gctttaattt cgctgattcc gccttcgact gtcagattgg aagttttgct
    16261 tgtgtcaatc ttcacggtaa catcggtatt tacaggtgtg tcaggtgata tttccacgtt
    16321 atttgaatag aacttcaaag tgtgtgcaaa tgccgattcg aaaatattgg catagatatc
    16381 ttttgtttcg gtatcaccga acaggttgat ataactgata cttgtttttc cgtttgtata
    16441 tattgcaaga ttgttgtgtg atgttgcata attgccgctc aaacctacaa tcttaaccgg
    16501 tacattaaag gtaagtgtct cgccgtagtt atatacaggt aatccgtcgg cattctgcgg
    16561 gtttgttatt tccgcttttt tgcccgctat cggttcaccg aaaacatacg gagactcggt
    16621 ccggttaccg gcggcatctt cgattataac ggtaacggaa gtgagtttaa cggtatcgga
    16681 gtacttaaca tatatttcag cgttaatatc tccggtcgtc gcattaaatg ctccgcttaa
    16741 cagaccgggc accctcttta tatcatacag agcaatacct gcaggtgcat cggaaccggt
    16801 aatatattcc gagtatgtgt cgtcaaactt cagatacacc ctgttaaccg attgatccgc
    16861 ttttgccgta agagtaaact tcccgtcacc cgcagcaaat acgggatcgg taacaaaagc
    16921 gggcggttca caatcgacat tggtaatgtg gtagcagttc atgtctgttt caccgccttc
    16981 tgtaggcaga acgacctcct tgccgttaac cagaacctta aaatagttgc ccgcatagtc
    17041 ttcgaggctg aaataaatat atccgttggt tatgaaatga tagaatccgt tttggtcagg
    17101 tctcactctt gcataatacc tgccgcccgg ctctcttttc ttgttgccat ctctattgtt
    17161 gaaccggtca tattcctccc cctgtatact gtcaaattct tcttcggtta tgtctcgcca
    17221 tgcttcaaac cacatttccg agcgcaatac atgctcgggt gtatcttcat aaaccgtacc
    17281 gtctgctcct gtctgtacat cataaaccga gttaagttta acaagtacct cttttaccat
    17341 ttgattggtt tcggatacaa atacatcaaa taccggcgca acattgtcgc gacgaagttt
    17401 aagtacataa acaggtgatt cggagcccct ttccgggttc acaaatttgt agtataaagt
    17461 tacctcacga acttcatcat cattaccgaa aagctccaaa ggtatcgcat agttgtccga
    17521 tctgaccgca gcataaaagt catattccca attggtctta ataggaacaa tatcggaaag
    17581 attgttcgca tcggttccta tatgaacggc taatttatat gtgttagcag gatcaaccat
    17641 gtttaacatg ggcgatctgt aatatgcact tccctggtag ccgtaatctc ctccgggaac
    17701 actgaaacgg atatacatat tgtttccgtc ctcttcatga ggcagtgtat tgatagggta
    17761 aacgggaata ttatcggtta tgtcatttcc atcattatca aaaaccgcct cgacattatc
    17821 agtcgggtat tttccgtata tgttataagg gtagccgata tcgtaattta tatcaaaaag
    17881 aataccggta ggcatatcgc agaataaata gtgtgctatc ggcgtttttg tcgattctaa
    17941 attgtttgta taacttaaat tacatacaaa ttcataatat gccgtatagc attctccgtc
    18001 tttatactca taatattggt gtgaaatata ttcgctgttc gggtcaaaat ccacaatagc
    18061 gagtttattg ccttcagacg tatttatcaa cgtcaattcg tttaacttcc atgtctctaa
    18121 aacagtgcgt tctacttcat gaccgcttgt aatacgagat tcgctcctaa gaaccttctc
    18181 caaagttata gagctgtttg caaaatcaat gcggtcaaat cctgttccgg gatctttact
    18241 gataacaaat tcagcctgag caataccgaa aagattaggg gtattaaccg gctgtccttt
    18301 tgaatactcc ggccattgac tgtcatcatc ggtcggccat atcagtttac cgtccgctct
    18361 taagtagtca acacgctttc ctgaattgtc attggtggaa aaacgggttt gaactgcgga
    18421 aaccatagga ggagcatata cagtattaaa ctccaccgtt tttacgaccc gcttgccgtc
    18481 atcgtcttcc gcaataatta ccaacatgat gggacgtgtg gtgttacccg gcgtagtgct
    18541 gtcatactta agcttttcag tatcgggatc cactttttgc actattgcat tatccgttaa
    18601 tgtgagtgtc ctctgtaccg actcattacc ataagtcaga tttgccccct gactttttac
    18661 aatcgcaatt tgaccggcat cgtttgctat taaactttcg gtattctcta cactgcgggc
    18721 aggtctttca gcccacctgt accaataaaa ctttgcatgc tttacagtga aatccacagt
    18781 gggataatgc cccgcttcat aagttgtctg ggtatcggca ctattaattt caatatcaac
    18841 atatgttctg tcgtattgag cggtagcctt cttaatagcc gtattaccat ttgagtcttt
    18901 tatctctatc catactactc tttcatttat ctcttgaggt gtcaggcttt cattcggagg
    18961 tggaggaatt aaagcttcct ctccgaccgt aagcggtatc cagtcggaat acgattcggg
    19021 tgcatcctga tcattcaggg aataaccgta ccggtactct gtggcggtat ccatatcttc
    19081 agttttgagt ataacctgtt ctgtcatcga ccaaaagtaa gcgaaagacc tgggagccaa
    19141 cctgtcaaaa ccgtcccagc cgggggagca caaggtatat ttgtttgtgc ctctgttacc
    19201 cgcattgtcc gttacggcaa cggttatatc caccttattc gtttccaagt cattaggtaa
    19261 cttgaaatat gcatatgccg cagtcacacc gccttcttta ctgaccggag ctacaaaaga
    19321 tgcgctcatt gtacctgcat caccttttct ccaggctccg ggaatatcct gataactctc
    19381 ggaagtagta ctctggtatt gcaataatgc agtcggtgcc gattttcctg cgactttgat
    19441 tgtaattgtc gcttttgctc catctaacga catatcgtca actaaagctt ttattccgta
    19501 aataccgctg ccctcatcgg ttacagttgt atttataaca ggggcaataa agtccacttt
    19561 aaactcgccg ttaggagaag gtgttaattt cagtttatcc tttgcaggta aaggaggaat
    19621 attcgtaggc tgaagaacac cgttgtcttg cggtatataa gtcataaagg tgccgccgcc
    19681 tctgtcctta aaccttgata tattattgaa attaatagaa gtaatcttaa gaaaatctcc
    19741 gtccagtata ttataatcgt catcgtaaga agtaagcctg aaaccgtatt ttacaatgtg
    19801 cgacttttgt ttgccggttt caactccttt aggtgataat gtaaggtatt gattgttttt
    19861 atttttaaga ttaagggtta tcgtcggata atccgtaaga ggaacatcct cggtgaatga
    19921 cttaaagggt gctttattta atgtgaggtt tatttcaata ttttggaatg ctttcaaaaa
    19981 cgccttgttt cgttcaccta taatttcaaa tgcggttatt ccgaaaggtc ttaaatccac
    20041 acaggcatag tttgaaaaag tagctgaaac cggtttgttc ttcgtagcat cgggctgtat
    20101 tccattgact gatgcgaatt tattacccga aaggtcgaat atgcactccc acaaatcttt
    20161 attctcatca atagatacat taacgcattt gaagtcataa tagcatccgt tctcatcggt
    20221 aggaactttg ggatcataac tgtacgggtc gggaagttga tattcaaatg tcataaccgg
    20281 tctcggagtt ttatccgttg atgtataact caggaaattg gcttccacta tctgaccgga
    20341 accggcaata cccattacct gaacaaacaa tttcagattt tcaatatttg cattctcctc
    20401 tttctgtttt ttgtataaaa caggctcgtc ccactctacc tgaaaataga tggtacgttt
    20461 ggcatccagt tctgcaatgg tttccaagga tactacaccg ttggttactt ctcttgttcc
    20521 tgccgcatcg gcagttactc ttatggtatt aatcttgggt ccttttatat ctctgcctac
    20581 aagcatagcg gcatacaaac cggaatccac attgtcatcc acattactgg ttgctttgaa
    20641 ccagagagtg tccagcttgt ccgcactctt ccaaccggca gtatcgccga atcagggacc
    20701 gctgttatct tccttgaaat aaccggtcgt gtggtttccc caggagtgta tctcttcacc
    20761 gaaaatctgt gtataaccgt catcacgact cttaccgtcc tgaccccacc attttgtctg
    20821 aacggttttt acatcccagg caaaaaagta ctcaatatct cctttgtcat acattccgga
    20881 aatatttcct ttatctcttt cttcctgttt ggtaagtccg tcaaaccaaa cccgaacttt
    20941 ccattcttta acattgctcg gacctttcag ttttgtctgc cattctccgt tgctgttaaa
    21001 actgctgccg aatttactgt tatccgctga gccgcgatta ccgaaatggt tgagaaggta
    21061 accgttgctg ctaagccatt ccatggattt tgcttcgtcg ttcgctgtac cttccggcgc
    21121 tgtcccccaa gcggtatatc cggcatcaaa aaacttaacc tgtttaccgc cggtatgcag
    21181 tgtttcatgg gcggaataag ttattgcgtg tccgtattta ttatattttg ttgtatcttc
    21241 ttcttctgcc gacacaatta tgccgggata aaaacctgcc ataccggcaa tcatcgccaa
    21301 tgttagaata aatgcaataa acctttttct catatgctta taaacctcct caagattccg
    21361 ttcttatatc ttctccgttc tgacgcatca gtgcggctat acgcgcaaga aaaaccggtg
    21421 ttctgtatgg cttttttatc caatcatcgg caccggcatt caaaattgcg ctttcttcct
    21481 catcgcttct gcctataaaa agcagtttag gcgcattact tattttttta accgtctcgc
    21541 agaatttcag acattccttc tcgtccgttt caatgtccgc aacaacaagc tgaatgtttt
    21601 tctgtttaat cagtgattca gtcgccgata tatccgacaa acaggttaca tcgtatccgt
    21661 ttcgtttgag actctttccg ctcagagctg caagctccgc atcgcgacat acaataagta
    21721 ttttcaaaca aatatctcct aaaaaaacag ttactctgaa ggaataataa tcagagcaaa
    21781 aaatcattcc tctatgctaa gtataatagg aataagtatg ttttttgaat taataggaaa
    21841 aactcacaat ttattttgtg gtgaaagtat atccttttcc gtaaaccgaa ataatgtcaa
    21901 agtcgtccgc attttcagtg tctattttct ttttcagatt gcctatgtga aagcgaattg
    21961 tgcgtgtatc tgctgttgac ggcatacccc agacagcctc atataattgg tcgggtgaaa
    22021 ggattttatc ttcattttcc attaaataga gaagcaaaga aaactctttt gccgttaagc
    22081 ttacgtcttt gccgttcaca aatgcttttc tttttgggaa atccaatgtg atggagcatt
    22141 ttgtaacaga ttccggttgc ccctgtctgg cttttacttc gaaatgacgt gttaagagcc
    22201 tgtccgcaac tgcaaggagt tcatcaaagc tgtacggttt cgtaagatag tagtcaccgc
    22261 cgcacctcaa tccctccacc ttatcggaaa tacgggtttt accggttaaa aatatgacgg
    22321 gattatcgca ttttgagcga aacctttcac atatatcata accgctgccg tcgggaagca
    22381 ttatatccag aatgagcaaa tcgggggtta cggatgacag acaacgataa ctttcttcag
    22441 ctgaacttgc cgtaatgaca ttatatccgc gacgtttcag caatcgtgca ttgatattca
    22501 gaatatccgg ttcgtcttcc acgataagta tggtaatctc tgtattactc atctgttttt
    22561 tctccataag gtaaagaaaa agataccgtt gtccctttgc ccaactcgct ctcaatccat
    22621 accttccctt catgcgcttc tattatctga cgggatacaa agagtcctat tccgtgtctc
    22681 catttttctt tattcaccga aatatatccg ccaaatactt tgctttttat ttcttcactc
    22741 attccttcgc cgttatcggt aaccgaaaca atctggcttt tgctattccc tttgagcgta
    22801 atcataataa atccgttttt tgtatgctgc atgctgttgg aaagcaaatt tgtaaccacc
    22861 tgtattattc ttgcactgtc cgctacaatc ggaggcagtt tttcatcaag tttcagaata
    22921 agccggtttt caccggtatt attcttgtta aaatagattt ctgtcgtttc acgcaacaga
    22981 tgagataaac gcacaggtgc aagatcaaga gagagccgcc cctgttcaat ggcaaccgta
    23041 tccataagtt caacaacaat cctgtcaata cgcaagacca ttttttcaat agtattttga
    23101 tttgctctca tcgtattaat atcgggatcc ttttcatcaa gcaactcaac ggtatcatgt
    23161 gcatataaat tgattgcatt caaggggttt tttatttcat gtgccacggt tgtcaagaat
    23221 accgattttg cctcactgta gtgttttaac tgctcgttct gttccgaaag cttaattctt
    23281 tgcgcttcgt attctcgaag atgaaagaat ataacagttc cgcaggcaat acataccgca
    23341 atagtagtaa aaataatgtc cgcaagcatt tcccactcag tggcaaacca tgtaacaagt
    23401 gtaggatttt tgtatgcaat aacggcaata ataacatatt ccgcaatttc ggcgaacgac
    23461 acaagaatag cccatttgcc ctccagcatc agtgtggtaa aaagcactgc gaatatgaac
    23521 agtgcaggtg tacctccttt gtaaccgccg gatgtaaaga acagcatggg aaagaaaacc
    23581 atgaaaatag caatgattgt aatgatatat ccggtttgat attttcccgt tttatatgtg
    23641 aaatatgtaa gtgcgaagga cagtacggct aacagtccgc tcactgcggc acttacccac
    23701 atcgaggtaa tcagatttac tacggtagcg gcaagactga ttgcggtacc ggcaatagca
    23761 agcacattaa acagtctgac gcgaaaatcc agttccctgt caaaaaaacg catcctcatt
    23821 tctttaattt ttttacgtgg gttaacagtc accagctttt tcattgataa tgcctcacct
    23881 tgccctaatt atattataaa gtgacagaag attgaatagt ttatattgta cgtatttccg
    23941 gaccccgtaa caacacacca tcaacgaaaa aggctgtata tgcccctatc atatttcccg
    24001 cttctattga cataccaaaa tacacctaat gccaaaccta taggtctaaa gatagttact
    24061 ataagatata acaactctga aataatggac atcaaaggaa cagtgtgtct gcgcaagagt
    24121 ctactgataa agccgtaaaa agcgttaaaa aagaaaataa caataatgac gaatagaata
    24181 acggataata taatcacaaa cttattattc ttttttttgg aaatttcact tacgaccaat
    24241 aaaagcggga taacaaaact aatcagactg aatattgcag gcccgactac ataatgaaaa
    24301 taggaagaga atctactgta gtcttcaagt atacttgtaa gttctctcat tccgtccaac
    24361 ttaaaaaccc ttataccctc gcatgtatca taagcacata taagtagaac aagtataaca
    24421 aaaacccaac cgcttttttt tgcgaacagc aatacggcaa gtatcataca caaaaccgat
    24481 aatataacaa accaagtgct tccgctgaaa tatctttcat gcaaatatag cctgaagaat
    24541 tgaataagag gtaccatcac cgacaacaaa atcatacacc ctgctaaaat attgattgtc
    24601 gattttcttg acattttcct tatcttcctc cgctcgtgta ataaagaagt aatttactta
    24661 tccgctccga aataaatacc gcctatatta tactttacca tataccgaat agacaagcag
    24721 cataaccggt tgacaagtaa aacaagctgc ttctaaccca aaggttgggc ttgttgtgga
    24781 caacaataag ccattatatt aaaatacggt ggtgccgagg tgtagcgcag ttggtagcgc
    24841 gcatggtttg ggaccatgat gccgcaggtt caagtcctgt cactccgacc aactaaagaa
    24901 agcaaatgaa agagcggttc ctgaattacc agataaaaag ctgcgttgct ctttaagaac
    24961 tttttttaca accttaatcc atttagtaag tgtacttgct ttcaaaacaa gtcttgcgtt
    25021 aataagttca ttttcaggat ttctactttc taaaaaatag tagaactctt tatcaacacg
    25081 gtgtttacga actaaaaaac taccatgtgc aaacgcatta cgaatgtttt gaaaaagatt
    25141 gggcattgtt tgatttgaat cgagtatgtg cacttctctc tactttgcaa gcgaaaattt
    25201 tgcagattaa tcataactta ataatccatt gacctaattt tgttgaacct tcatattgaa
    25261 taagtccggc ttttttcatt tttcctaaat gataacgcac accatcaaca ctaaggttta
    25321 actgttccgc catttctctt gcacttatat gagggttttc agatattagt ttcataatat
    25381 catcttgttt cgtagtaatt ttggtggtag ttttagtagt agtttcttgg gtagtactac
    25441 cactaaaacc tctcaaaaat tccgcattta ttcccataac cgcattagta tagttattgt
    25501 actcattatc tggataaaac ttaagctcgg gggaaccgtt ttctaacaaa gcatcttttg
    25561 tccgccttat gcctgatccg taagattcaa ttaaattcag cgaaaagaac atgtctttga
    25621 gttctttatt aagatactgt ctcctgtcaa aactcctatc tcggttaaga gcttcaattg
    25681 tgacgggagg aagcggtcta ttgtgattta caaaggaaat cctgtcctta taaatataaa
    25741 ttcccacata ctcaggtgtg tcaccttctt tttattcctc atgtgtatat catagcattt
    25801 tcaaatctct ttttatactc cagactttag ctatttaaag aatatgcggc ttatctgatg
    25861 cctgtaaaca ttgattttat cagttttcta aaaaaatccc gtaagagcac tcatctttag
    25921 ttgcaggcaa agaatttttt tctcgccaaa acaagaattg atgcgccaaa aactaaatat
    25981 tgacgagaat aatctcgcca atattgattt atctacgcca tataaggtat aatggctatg
    26041 ataaacagga ggtgatgttt tgagaaagtt cgattattta aaattgatga acctgtcgtt
    26101 gccggtcagc atttatcata cgatagcaca aattcacgag tataaaggca aacaagaact
    26161 ttatgtggta aattatccgg atgtcttaga gaagatgata aatatcgcaa aagtacagag
    26221 tacaaagtat tctaatgcta tcgaaggcat ttataccaac gatacaagac ttgtagagtt
    26281 aatgaataag aaatccgagc ccaggaacag aaatgaggaa gagattgccg gatacaggca
    26341 tgtgctcgat atggtgcacg aaaattatcc ttatatccaa ttcaacaaaa gtgatatttt
    26401 aaccctgcat aatcaattat attctttttc ccatgtgaac cataagggaa agttcaagac
    26461 gatggataat accattgttg aagtcgatag cttggggaac agaaaagtaa gatttagacc
    26521 ggtaagcagt tttgaaacgg aaaagtattt cgatgaaatg gtagaagctt atagaaaagc
    26581 cgttgaagca aatattccac cgcttatttt gatacctgtt cttatccacg attttttgtg
    26641 catacatcca tttgacgatg gaaacggaag aatgagcaga atattaacac ttcttctctt
    26701 atataaattc aactattttg tcggaagata tataagtatt gagatgctga ttgaagatag
    26761 caaaaattct tactatgaag aacttcaaaa ttcaagtgaa aaatggcata caggcgagaa
    26821 tgacgaatta ccgtttatta aatacatgtt ggggattatt ctaaaagctt atgaagagtg
    26881 tgacaataga tttaagctga taggagataa gacattatca tcacctgaaa gagtattggc
    26941 agttattcaa aaatcgctgg aacctctttc taagggagat cttgtaatgc tttgtcctga
    27001 catatctcaa aggacaatag aaagagcctt aaaagaatta aaggaaaacg aaaaaataag
    27061 acaagtaggt agtggacgct caacaaaata tatcaagatt taaacaccct tttcttctat
    27121 ctttattata aagtcatttt gttttgcttg acaaaccggg atttgtctgt cttttttcta
    27181 tcccgataaa ctgaaattta tctttttttg ccgcgaataa tcattataat taaaacagat
    27241 gtgtcccact gttttattcc ctcgccgtac aagcgaagtc gctcgtcaaa cttagacttt
    27301 actgcatcaa ttgctctttt atactctgtt gaatgtgctg atgcaattgc aatagactcc
    27361 aaattagtct gacgcattga tttgattatt ctttccccca ttgtaaccga atattccggt
    27421 ttgtcgggag tcagattgat aatagcataa tttgttgtaa catctttaaa acaatgctgt
    27481 tccatagaag caggaagctc cgcttcagac ataggatatt ggcatactcc atattcttcc
    27541 agtatctttt ctgaggtaac agatttccaa aattcttcct cttcttttgt gattttcaga
    27601 cacggcgcaa tacaagacaa ttcctttctt gtagaaaggc aaaggcatat accgcccggt
    27661 ttcaaaacac ggtactgttc actcaaaaaa gcattaggct caatatgctc ttgaactgta
    27721 ttggatatcg ttacatcaaa tgtgttatcc gcaaacggaa gatgttctgc atctccttca
    27781 atgaattcaa cgcctgccac attatgccta ccgaaagaaa taaagttact atctcgatca
    27841 atcgctgtta tttgagcatt cggataccac ctatgcatcg cctcagcaag cgcgccgggt
    27901 ccacagccga tctctaatat ctttaattct gcatctttgt ctatattaaa agaatccctg
    27961 tattggcaaa aaaacacatc atcaaatctc aatttacggc tgagataaag agtctgaatc
    28021 ccttgaacat tatctgacca tatagtattc attagcacct ccacaaattc tgatttgtct
    28081 atatattttt atagcccatc ttttcggcca ggtctccgag actcttgttg tactgcgcct
    28141 ggctgattgc tttccgttcg agaaagaggt ccggcgttct tttctggtcg gagtgacagg
    28201 acttgaacct gcggcatcat ggtcccaaac catgcgcgct accaactgcg ctacaccccg
    28261 gtgctaacac attttactac aaggcctgat tattgtccac tgtgacatgc aggcggtttt
    28321 ttgtgttttt cgggaaaaaa agaagcccga aggcttcctt ttatatatcc ctttctataa
    28381 gatcggtttc ggtaacctgt ttggttttta catcaaatct gaaggatctg acctgatact
    28441 tgtccagtgt gaattctctt tcccatctga aaaggggtaa tgacagtttt gtagtcttct
    28501 ttgtatcggt cggattgaac aaccttatta tatacccctt tcggtcttcg cttcttttga
    28561 ataccggcat ggtgataaca tcgtcactca gtatggcaaa cggtcgcacc ctctttccgg
    28621 cacctgtcgg gaagaaagat aatgccatgg gtttttcatt acatacagcc gcttctcttt
    28681 ccactttgtt gaatctttcg gttgtttcgg aagcatccag ccaaaattcg tattgtcttt
    28741 ccccctgatc catatacggt gaaaatctgt cctgcggcat aatgtatttg ttcctaaccg
    28801 aaaagtcgga ttttcctgct gcatagccgg ccgatctgag aagcgaaatc cttatctctc
    28861 cgtccaacac atcggaaccg tatgtgcagt catttatgac actgaccgca taatctttgt
    28921 tatacaggat attccattta tgactgacat tttcgttacc gtcggtaagc agttgttccg
    28981 aaccgtaagc ctgctgtccc gtataacaag cattttctat tgttgtggga attgacagtt
    29041 taagcatttt catcttctcg ttccagtata ctcttacagt cactttgacc tgtgtacctc
    29101 tctttggcag atgatatctt atgcatatga aggagttgtc atacttcata accgcttcaa
    29161 ttatagttct taccggaccg tcttcaattg ctctgacgct ttcaatcttg ttctcatcaa
    29221 ttatgccgga gaaataatta ccttcttctt tgtccatgac tttgaagaat ccttcggtgt
    29281 ttctgaaact cttttccttg tgcgcccagg agttttcatc gtctttgaca actacgggaa
    29341 gaaaagcatt cttctttaag tagtctatgc cttttacttc atacttatct attgttccgg
    29401 taagcttgga aatatgaaca agcaattctt ttgtcttgaa tatgaacata tcctttgtct
    29461 gtcttacctt aggcaacggt tttgcatcaa tcatctctat tctgacatca aacctgttta
    29521 tctgacaggg tttcagcata gcgtagaata ccgatttctt tctccagtca acattgaagt
    29581 tggagtcttc tttctccacc tgacagggca gtttcttacc gtttcggtaa actacgggat
    29641 tggcaaatat atttctgttt ttattctggt ccgccaactg atattcacac tcgacaatat
    29701 acggtacctc ataaggatga ggattgtata ccagcaacgg aatctctcct tcgtttgctt
    29761 ttttctgacc ggagcacagt gaaatgaagg cctgagtctt aactttgccc aattcatcca
    29821 aaccgtgtcc cgctaaagct atggaatagt cttccaccga ctgtattgag gtgcccggca
    29881 atatgtcatg gaattccata aacagcatgt cttttgttgc cgtccgtatc ctgtccgcag
    29941 gatacttcat aagttttaag aaagatgcat gtgcagccat cttttccgtt gtatacaact
    30001 cgttttccaa ctgtctgtgc aactgtttaa tgcgaatctg cgatgtatag catccgatat
    30061 acctcggatt aagtgtcttt ttgaataccg gcaacttttt ctcatccagt tcggcaaaat
    30121 atgcttcggg agtggaatga attatattga agtccttctc cttctttgcc agttgtgcta
    30181 tgtttttaaa atcaattctg gaaggtccgc cgccgtgatt tcctataccc cacagtacca
    30241 tacccacctt tctgtggcga ttcttatcca tccaggattt tatcttttca tgcgcttccc
    30301 ctctgtgcga ttcataagaa ttgaaagcac ggttacacat tatggtttct ccgccaaatc
    30361 cttcccaaag aaaatcctca tcgggaagat caatcatgtc cggctcggga cggcagaaca
    30421 gataagagtc atatccgctg ttttttaaaa tatccaccaa cccccttgta tgtccgaatg
    30481 cgtcgaaatt tatacatgtt gtcgggaaag taccgaattt ttccataaag tagttgtgtc
    30541 cttccaatat ctgtcttacg aaactctctc cggtgggaat attgcagtcg ggttgaataa
    30601 accaacctcc catgatatgc cattttttct ctttaaccag tttctgaata cgtttgaaaa
    30661 gtgcaggctc gtatctttcc acccattcat acagcagcga ttcattgtga ttgaatatga
    30721 atccgtcata ttcttcacaa aactcggcac ataccctgaa tgttgaaaga gtctccgcaa
    30781 taccttcatc aagattccac atccataccg gatcaatgtg tgcgttacaa actaaatgca
    30841 gatttttcat agttaactcc tttattgtta tcttaatata ttttaataca taagccctat
    30901 ttttaacagt gtgtatacat aatgttgctt tgatgtgtta ttatataaca aaagtaagta
    30961 agcatttata ctgtcgggag ggggccatga agcatctgtt tataatcaat cctatgtcgg
    31021 gaagtaagaa agccgcaaaa ttcctaccgg aaataaagaa atattttgaa ggcagagagg
    31081 atgagtacag tatagtctat acacagcatg ccacgcatat aaaagagttg tgtgaagagt
    31141 atgcaaaaaa gggcggatac aggatatatg ttgcaggcgg tgacggatcg gttaaccagg
    31201 ccgcaaacgg agtggcaggt tatgataccc cgataggcat acttcctacg ggaagcggaa
    31261 acgacttcat taagaatctt accgattaca caaaacttaa agacctcgtg aaaaggaccg
    31321 ttgaaggcga tgtgattaag gtagatgcta taaaactgta tgatgaggaa ttattcggtt
    31381 attgcataaa tatattatct atgggtgtag atgccgaggc agcttacaac tctacacaga
    31441 ttacaagaaa atacggaata accggaatgc ttgcatatct tctgggtttc ttcagagttt
    31501 tacgacagaa aaaaaccaaa ttcgatgtca aaataaccat agacggacaa gtaataaatg
    31561 acggctatgt aatgctggca gcctgtacaa acggaaagta ttacggcgga ggattcatac
    31621 ctgtgcccca cacatcattt gatgacggaa taatagatat gtgcattgcc gaagccagag
    31681 gtctggattt cgtgttttcc gtattaccta aatatatgaa gggcaagcat gtaggtgtgg
    31741 aaggcataac cttccccaaa ggaaaaaaca tatccttaga aagcaaggat atgcttaaag
    31801 tcaatataga aggagagata atagaaagaa ggagcatccg aatggaaata gtgccccgtc
    31861 ttataaatgt gattaaacct aaagatttag aataataatc ttattcgaat ttttcgccgc
    31921 acttcatgca tgcaacggct tctttgggca tttcagcctt acagttgccg catatcttca
    31981 tacctttagc atcggcaata tccaccttta cctcttcaat ctgctccagt ttatcggtta
    32041 tctgggtaaa aatttctttt gtatcttcgc cgaagtatgc cgctttgcct gctatctgat
    32101 aaaggttatc cagatccaaa tccaattctt tcaactttat tgaaagtttt gtggtgtcgg
    32161 caaattcttt tgtttttttg aaaaatctct tgcctgcatt ttccagtctt gtgcccaaat
    32221 cttcattctt ttccatgttt cacctcctgt gataattata ctttactttt tctcttttaa
    32281 caaatctctt atttcagtta aaagtacctg ctcttcggaa ggagcgggcg gcgccgcctt
    32341 tacttcctct ttctctttgt ttttctgctt cgcttcttca gctttctttc tcaccaatgc
    32401 cacaaacctg accaacagga atacacatgc cgcaatgata agaaaatcca atatgttctg
    32461 aaggaatgtt ccgtaattca ccgacgatgt aatattgccc tccgcatctg tagccaatac
    32521 ccatttcagg tcattaaaat taaccttccc cattaccaac cctattaacg gcatcaacaa
    32581 atcatttaca aatgaagata ctatctttcc gaatgcggct ccgataatta cgcctaccgc
    32641 catatctatt acattacctt ttgttataaa tgccttgaat tcgcttaaca gattggtttt
    32701 gttcttctta gccatacact tatctccttt atatattccc tgcaacttaa ttttacactg
    32761 ttgaaaaaca aatgcaacaa tatttaagag ctcttacaga ctgtttctta tcagaactgt
    32821 ttttatcccg atataagaag aaagaccgtc aatcatgcag aacgccgact tcggatcttt
    32881 caaagactca gccggcttag aaaaaggttt ttgcctctac cggctctata gcgtttttat
    32941 tatatcatta atattttcct tttcaagata ttgtttcgaa agttcacaat tgttcataat
    33001 ccggtgcaca taagatttct tgtataagct tgtataagat tggcttaaat tactgttttc
    33061 acattctata tatcaccgtt atgatgtata atatatatgt taaaaaagca ttgaaaggat
    33121 gcgttggttt atggattatt atgatatcaa catcgccgga gtgcaaagaa gattaccgtt
    33181 atgtaaagtt accgacaatt tatatatagc cgcttttgtg gttttcggag atatagagct
    33241 gacaatagca tgtgcaaaga gacttttaga acttgcaccg gaatatgatt acaccattac
    33301 cgccgaatcc aaagggattc ccattgtcca tgaaatggca agacagagcg gagtcaataa
    33361 atattttctt gcaagaaaag ctcccaaact gtatatgacg ggagttttcg aggtggatgt
    33421 gaaatccata accacatccg gtcttcagaa actttatctg gacaccgccg atgcggaatt
    33481 tatgaaggga aaacgcatcc tcattgtcga tgatgttatc tccaccggag gctcattacg
    33541 tgcagttgag gaattggtac tgcaagccgg cggaatagtt gccggaaaaa tggcggtttt
    33601 agccgaaggt gatgccattt atcggaatga tataacatac attgctccgc ttcccttgtt
    33661 caatccggac ggaacaataa aaagatgaca ggaaaaaaca aaaattcaac aacagttaat
    33721 gctaaaagaa aagctgtcat aatagttttg tctttgcttt tgttattaag cggatgctta
    33781 aaagaatctt cactgtatat aacggaaaaa aacggtatgt ataccacttc ctttatagca
    33841 atggataccg taatgtcggt ctcggttata tcaaaaaatg ccgaaaaaga attgcatgaa
    33901 gcatataagc ggattctggc atatgatgtc gccttttccg ctcactcaca aaacagcgaa
    33961 gtatcaaaag taaacaattc atcgggtaag gatatagcta tatccgatga acttaaagaa
    34021 cagataagca cggcattgaa agtatcaaaa atgactgacg gagcttttga tataacggtg
    34081 tttccgctga tggagctgtg gggattctat gataaaaacc ataccgttcc ggatgaggca
    34141 agtattaaag atatcctgaa acttgtcgga tatgacaaga ttggtctgga aggtaatatg
    34201 cttaatctgc aatcgggtac aaagatagat ttgggagccg ttacaaaagg ttttgccgga
    34261 caaaaaacgg cagcaatgct tattgattcc ggtgttaaat caggcatgct ttctttgggg
    34321 ggcaatattc aactgttcgg acttaacccc gataaatcgg tgtggaatat agccgttcag
    34381 gatccgaaag acgagcactc ctatatagga ataatccatg atacagattg ttctatcata
    34441 acttccggtt gctatcagag atactttgag aaagacaata aaatatatca tcatatactc
    34501 aatccgcaca ccggctatcc tgccgacagc ggtatccttt cggtcaccgt ggttgcaaat
    34561 gacggtacat atgccgatgc gttatctaca gccctttttg taacaggttt agataaagct
    34621 gttgaaatat ggagagaata cggggatttt gaagcgatct tcgtaaccga agataagcat
    34681 gtatatatca ccgaagggct gaaggataaa tttactccgt attcctgtca agataagacg
    34741 gacacatacc tttatatata cattaacaaa taatcaccaa tacctgtcaa cggtttgcga
    34801 taccgtctta acccattccg acagatgatt tacatttctg ttaaccgtgg aaatatcttt
    34861 caatacaaat tcgcaaggcg tgttgtttct cttacatgca agaagtattc tttttgtctc
    34921 ctcaataacc ggttgaggat caaatactgc ttgcgacaca tatgcgggat tggattttgc
    34981 cgaaagtaca tatctgtttc ccatggcatc ggctgcaatg tcgacatccg cccagggggt
    35041 tacggatatt ctccgcaaat tatcaaactc cgttaaaata tcaattttgt tatgtaaagg
    35101 ttcgcagcag ccgtaataag taagtccgca cctgtcaaac agccgcttca tgtactttat
    35161 atcaaattct ttgtgcatat cgggagatac aacggcaaat atctgagcca ttccccttcc
    35221 ccagacatcc ttggcactga cggtttcttc ggccacatcc tttaccggca gctcataggt
    35281 gcatgccggt gtacagtgta gataatatgc atcggtttcc aatatattca gtttttcata
    35341 tagactgacg gtttcctcat gataccgtgt aaacttatcc atcatcttat gaataaaatc
    35401 cggtctttca tatatatcga taagacaatt ctccacaccg tgatatcggg gtatttcatc
    35461 ccacatatta aatatgagct gatagccgcc aagccccaca tccattatgc cgtcaaatac
    35521 atcatgagcc gcttccagtg ctctttgcgt cgcctgttca tccacctgca tcgtaggcat
    35581 agttattttt tccaaagatt cctcatcggg taaaatatca tgatactcat gagcactgat
    35641 atcggtcccc gtggcggagc caatcttaac ctcctgcacg gtaagaccta ttccggtacc
    35701 ttttgttctt atccttacct tgtaatacgg atgtattgca taatcgcccc ggaaatactc
    35761 gaactggtaa agagctctct ttacggctct ttcaattcct ctgtacaggt cggtttcaca
    35821 ttccaaccga agtttttcct gatcttccag ttcaccccac ggcacttcaa aaacaagaac
    35881 gggaggtcgc acctgttgca ggctgttaag ttttctgtat tgctccggta ttgccagatt
    35941 actgtcgctc aatgctattt cggcatattg ttttgcatat tctcttaact taagaagttc
    36001 gggcttcata tatcggtttc ctccacaact tcatgaactt taatcagcct gtttttttct
    36061 tcatacacat tctataatat tgattctaca ataaaatcgt cgtcaattaa agtataacag
    36121 tttttatcct tctcatactt ttgttaaggt gtgcaccgga atatatgccg aagcttcttg
    36181 aataaaaggt attaaaatgt tacagttaat gaaaaggaaa aaacgacatg ataatagatt
    36241 tccacactca catattcccc gatactcttg caaaaaaagc caaggagacg cttttgagaa
    36301 acgtaaacta cagatatgag cctgctaccg atcttacaaa ggcagggctt attgaaacaa
    36361 tggataaatg gtgtatagat atatctgtga atcagccggt gataactaaa agaactcaac
    36421 tgcaaagcac caatctgtgg gcacagagtg tttgtgatga cagaataata tctttcggag
    36481 gaatctttcc tcatacggaa gattacaaaa aagatataga tttcgtctgc ggtttaggct
    36541 tgaaaggtat aaaatttcat gtggaatatc aagccttcgg tgtggatgat ccgaaagcct
    36601 taaggatata tgattatgct ttgagtaaag gcctgataat tgtacatcat gccggtgaag
    36661 atcacggtat gcctcccccg tacagaagta atccgcaaaa attcgcatat attgccgacc
    36721 acatgcaagg aggcatcatg gtggcggctc atcttggcag ccacaacatg tgggacgatg
    36781 tggaaagata tcttgtaggt aaaaacatat atttagacac ttcaatgggc tttgaatatt
    36841 acggcaagga acaattcttg cgaatagtga aggctcacgg ggccgacaga atactgttcg
    36901 cttccgactc gccctggagt aatgccaaaa atgaaataga tgccattaac agcttaccta
    36961 tcaatcaaac ctctaaagat ttgatattag gcggtaatgc cgcaagaata ctgggattaa
    37021 gttaaatttg aaaggagcag gagctacaaa ttatgaaaaa aaataagcag acaaaaattg
    37081 atttcgggta cgcattttcg gaacctcaca gaatatgtgt atgtcttccc aattcgagta
    37141 acaagacact gtatgactgt acaaaagatt ctttaaccat gctgtggagc gacagcgaca
    37201 caacacataa tcctttgggt gcatacaaaa gagttcgtct ggactggaaa gtcaagataa
    37261 ttgcaaagtg taacgatgtt gccatggaag ctttttcctg ggagagaatt gagggatata
    37321 taccgtcact gttgtataaa gcaaaggcgg aaggtgcaaa ccttaccgta caatgtgtgg
    37381 ctacaagact cggagatgtt attaaaatgt ccgttaccaa tacaaccggc agcgataaga
    37441 atatatttat tgacgcatgc gtattggaca acacaattaa taccaagtgg gcggattttg
    37501 actgcgaata cagcgtggta aatcccatat tcaatgacgc ttcggacagg atagttatta
    37561 tggatgcaag tcttaccgat actgttcctt ccagaagaga gtcggtcaca tatgcatata
    37621 cactcaaagc aaaagaaacc cgtgagagta tgcttatacg ccccaataaa aaatacaacg
    37681 acgatattga agttatgttg aagacaaact ggaatgccct gtacgaacag gggctgaatg
    37741 agtggagaaa tattatcaaa aaagcaccgg tatttgattt acccgacccc atgattgaga
    37801 atgcatataa agcgtgcctg tgcgatatat tcgtaatgag agaagaaatg gctgacggca
    37861 gaatggcggg acttgccgga acggaaatgt acagaagctc caataccgga gagccgtgtt
    37921 tccaggcact tacattgggt aaatacggat tttttgagcc tgcaaaagaa aacatcgagt
    37981 tcgtagcaag ttttcagctg aataacggct cctgggaaga tgataagcaa tggggcaagt
    38041 atatgtgggc ttcatcagga tggaagagcc gctgtatcca gcactactat ttacggacaa
    38101 aagacagagc atttttagag gagaattttg aaagaatgct tgcctccgcc agatggtcca
    38161 aggcacaaag agaaaaaacc aaaaagaaaa actctcccga ttcacccgaa tggggcttaa
    38221 tgcccagagg catgggagac ggcggtctga aaaacggcga tgaccttttc ggagtttttt
    38281 atcctcacaa cttcatgcac tgtatgggat tggaagcagc tgtctgggcg gctaaagagt
    38341 tacaaagaga ggacgtttat gatgagttgc ttgccactta tctggaccac aaagaatgta
    38401 tattaaagtc actgcaaata ggacatatca aagagccgga cggttcggtc tggataggaa
    38461 gctgtgccca taatacatca ggcagcagat gggctgtggc agatgcggtt tatccgacca
    38521 atatactgcc cgtggaccat cccttagctg taggtaccat gaataagtta caggggaata
    38581 taagtgaggg agggttgccg aaaaatctcg gatggataca tgacggttta tgggtggcta
    38641 tagcattgga tgccatggca tatgtaaata tattagccga taaagcggac atatcggccg
    38701 attatcttgt agctgcactc aatcacggca ctcctctgtt tacctggtgc gaggagcgaa
    38761 ccgaagaaaa aggagcaaaa agaataacag gagatctgca acatgcatgg acacctatat
    38821 gtgttgctca gtttattcgg gatatgcttg tatcggatta tctgtttaaa gatgatatgt
    38881 tgcacttaac atgtgcggtt ccgagatatt tctataaaga aggcaatgca atgaatatta
    38941 aggaggcacc ggcattccaa ggcaaaatat ctttcagtat agaagtatct aagaatacgg
    39001 taaccttcac tgccgatttg tgttcttttg atattaacag agaactgtgc ttacatttac
    39061 ggctaccaaa cagaaactta gaagtaaccg tacaaagcat atcgggaggt tcggcagtta
    39121 ttaccgaaca aaaagccgtt ataaagcccg atacaaataa gattaaagcg gtatttaata
    39181 taggctaata cttttagtta agataattat taataataat tactaatacc ttgcattatt
    39241 gacgcattca tatccgtaat ctatgtatgt catgatgtta tcaagatatt gcttgctcgg
    39301 tgtcggaaaa tccatgaagc cggcggaagt acacattata aatctgttgc tttgctttcc
    39361 gctttgcacg caagttttta ttttttccct tataacttcc tcggattcat cataaaaatc
    39421 ctgcatctct atattgcctt ccagacctat gcgattcttg aatgttcttg ccacttcgtc
    39481 caaatccaca tctccgttct tgggcggttc caaaggatta agcacatcga cacccatctc
    39541 aataaaattc tctatcaggt tccgtacttt accgtggcaa tgcacccaga tgtaacttcc
    39601 ggcattgtgt accatatcgc aaatgtgttt atcataatca aaacagaaat ccatgaaatc
    39661 tttttccgat aataacggag gaataagcaa ctccggtccc acccaggaaa aaggtactct
    39721 gacaccatga ttaagcgctt cttcgacatg agcatacagc cttttggaaa aaacatccaa
    39781 tgcctccatt aaaaggtctc tgcagtcatg ggtaaagtat gcaagatttt ccgaacctat
    39841 catacgataa agggcatatg cgatgtgatc aatacggtac aggacaattc ccctgtctcc
    39901 caacagttgt tgctgctctt caatattatc aatatacgga aacggctcat acggcataga
    39961 cagcatcctc tttatatcct ccggttgttt tacgggatat tctatcgtaa accccggttc
    40021 acctattttt gatattctgt ctaccctgcg aagttgaccc ataggtgtat ggaaaactat
    40081 atgctcctcg taccacagag gatcatttat ttccacattc tttctcctga ctatatccac
    40141 ctgtccgcag aacatgaaaa acggagtgct cgcattgact acaagatcag tcttttccgc
    40201 ggcctttatc attgccggct catatgaagg atgcaataat ttatcaccct tacggtaatt
    40261 ccatgatccc cacaacttta tcgcgggcct gtctatcggt ttattggcaa aaatcctcat
    40321 taatctttct tttgatgtaa gagacatatg ctactccttg ttcaagattc tgttatatgt
    40381 attatatcat gaaataacac aaccttattc ctctgccaca ttacttttaa caaaacaagg
    40441 ttaaatatta aaatagttat tgacatatgc cattaatgtg gtatactgat agtatccggc
    40501 atatgccgga aataaataag ggaggttcaa aagatgccca gatttgacgg aacaggaccc
    40561 agaggaatgg gcccgctaac aggaagagct atgggatact gcacaggcgc cagacgtccg
    40621 gtaggcagat actatggcag aggcggaggc agaggaagag gatttggcgt atgcaacagg
    40681 ctgtattact atgatgatgc tcctctatat gatgaaaagc cctcaaagga acttctgctt
    40741 aatcagaaga agtttttaga agaagagctt gcatcaataa ataaacagat ttatgaatac
    40801 gaataaaact tacaatgctt aagtttacat aagttcatct taagcaaagt attttaactg
    40861 cctgaaaaaa ccgtttcggt acgatatata gccgaaacgg tttaattcaa caaataaatc
    40921 tgttctttac tattcaacta atactcaata actatacggc cctcaacttt cggatcggca
    40981 agcaccattc ctaaaaatgc ttcttctaac acatctccgc tggaggtggt aactactttt
    41041 ataggggaat tggcttccgc ttcttccttg gtgataccta taaagtccgc catattgtta
    41101 aaatggtact cctgtaaacc taccgagtaa atatgattat catccaaagg ttgggattcc
    41161 aatgaaagtt cggtaactct gttctctttt ttggaatact ttaccttgaa ccctctggac
    41221 acctgataga attccacatg gtcgcttaaa tctcttccca aaattgtgtt gaacgctttt
    41281 tctatctgct tctttgtcaa ctttattctg tatatcttgt catcaaacgg gaaacattcc
    41341 ttaaaatcct taagtgtcag tataggcccc aactcttttt ttcttatact tcccgacgca
    41401 aacaacatta tatccaagcc gaggttatcc actaccgcat ccgcacataa attgcccaac
    41461 tcggtttcct gacttctttt cggatgggtt aatgcacgct tgaatctggt tattattctg
    41521 gagaattttt cattggtttt ttcatgatac tgaagtatta tttcttccaa atctttatct
    41581 accgggcaat gcgtctcatc tatgggtacc gcctgccagg taaaatcttc tatgcagttg
    41641 ttgtctttat caacaataat atcaaatctt cctatctgat cggtacctgt cccggcttgc
    41701 gcaataacta taccgtttac tatatccggc tgctctaaga atgtatgaga atgtcccccg
    41761 attattatat ccactcccca agccggatcc agcattgaag ctaatttctt atcctcttca
    41821 tgaccgatat gtgtgagaag tattgtgaaa tcaatatcta tttgagaata ggcattacaa
    41881 attcttccca cttcctttgc ggcttcataa acatccacta tggaacctat aagagtctcc
    41941 tgtttggtct gagcaataac ttcctccgtc aatatgccga tatacagtat ttttatcccg
    42001 cctacttcct ttatgagata tggtctaaaa agacgggtat gattggtttt tatatataaa
    42061 ttgctgttta taatagggaa cttggccagt ttctcaataa acagaagatg ggcaacaccg
    42121 taatccactt catgatttcc caatgtaacc acatcgggtg aaagcatatt aactatttct
    42181 attgtggaaa gtcctctgta ttcctgatca ataatggagc ctctgaacat atctcctgct
    42241 ataacgtaaa ccacattttc ttcttcctgt cgtactttat caatataccc tgagagcatt
    42301 gcaactccgc ccacaagttt atcgtcaata ttctcgctca taaaatctcc gtgcatgtca
    42361 tttgaatgca gcaaagtcag ttttctcaaa tctcttccca taacaacctc ccattctatt
    42421 tattttccta ttcaaactta tccggacaaa tctacatctt gttagattat atcatcttca
    42481 catatcctcc gctatacggt ttcaaatctt tttgatatta cttatcgcgt ataacggcag
    42541 attaataagc cgctcttctt ttttatagtc cgacatggat gtcctgaccg aaatctctat
    42601 atcatattta tcacgaaatg ttttaagact tttagattgt aaattaactt cggcttttac
    42661 ttcaaccgga ataaccctac cctcactgcc aatcagaaaa tcaatctcgg ctcggtttct
    42721 gtcattggtc caataataga cattcaaatt atccaatgtc tttaattgct gcagaacata
    42781 ctgctccgtt aaagctcctt tgaattccct gaacaagtca ttaccgctta acaaagcact
    42841 ctgatgcaga cccaccatac aggataagag acctacatcc aaggtaaata acttaaaagc
    42901 ttttaaatcc tcatatgcct taaggggtat attaggcgca ttaacacgat tcacctgatg
    42961 gacaaggccg caatcggtaa gccacaatag tgccgtttca tattcttttg ctctggcacc
    43021 ttctttaatc agaccgtata taaacttttt attctccttt gcaagctgag ccggaatact
    43081 gttccaaagc atgcgaatgc gggatacaat ctcattggga gcatgcttgg aaaaatcctg
    43141 ctcgtatgca tctaaaatac gtctctgtat ttcccgaacc ccttgaaaat ccttattaac
    43201 agcgtagtgt gcaacaacct caggcatacc gcccacatag aaatattgtt tcaaaagatt
    43261 aatgtattcc tgcttaaaag ttgtaatcat ctcatagtct tttgattcca acagttcggc
    43321 atactgttct tttcccattg ccagcataaa ttctgaaaat gacaaaggat aaagatccat
    43381 aaactcgact ttacccaccg gaaaagatgt tccgggatgc agtgcaatac ctaataaact
    43441 tcctgcgcat acaatatgat atgaaggagc atcttcatta aaatatttta aagaagtcag
    43501 tgcattggga acttcctgaa cttcatcaaa tatgataagg gtctcggacg gaactatctt
    43561 ctttccggta tacagttcca gtcccattat taacctatct acatttaaat cctgcaaaaa
    43621 cagctgcttc atttgcatat tgttatcaaa attaatatat gcatgcgatt tatatgcttt
    43681 attcgcaaat tccttcataa gccaagtttt tcctacctgt cttgctccgc gaataatcaa
    43741 cggctttcta ttgtcttttt ctttccattt atacaaatat tctaaagaag tacgatacat
    43801 aaccgacctc ctttcgctca caatagacat aatatcagca ttttacaaaa tgttcaacga
    43861 ctttttgtgc taatgcaaca ctttcttcag cgactttttg taatatgata cattttattc
    43921 aatatttata acttgaaaca tatgtaacac aatgtattac gagaaggtgt tgatatagat
    43981 tcattgataa ataagtgaaa gatctttatt ttcataaaat acagctatca cacaatgcac
    44041 ggattgattt tattactgtc tcccatgtga tatctgccgc atagctattt ttcctttggt
    44101 aactaatcca aaattcccgc atagtaggat tattttcaat ttctttcaaa atatatcgat
    44161 aatccgttaa taccttttca ctgttccgat tacgacaggt tgctgtcagt gcaacggtaa
    44221 gtatatttat atcaatatcc attcgggtgt tttgtaggat atatatatcg taaaaatccc
    44281 gtagtcttgt gttaaagatt gttcggatta atacggtttc gattttttcc gcaagtatag
    44341 tttcaagaga gtatgcccgt ataggaatat atcgatgctc aaacatcagt ttgtagctat
    44401 atgtaatttc tttcggtatt attgaatccc cggttgatat gtcaattcta aagggaattc
    44461 tcatattttc taacaatgcc tcaagtgaga gacgtacaca gatgtagtcc gcttcatcca
    44521 ttatgtttga aatatcctta acagaaaaag ccatattgtc accaatctct atggcagtga
    44581 tatcctcaac aattatcctt gcatgttcag gattaagggg taaattgagg attgtagcat
    44641 ccatatccat tgtcgcccgc atatccaacc caacaataga ggaaacgagc atgccacctt
    44701 ttagaatgaa attattccta tactttgaca gagcgatacg ctccaaaaac cgttccattg
    44761 cataattgcg gaatagcagt tgcgatttac cgctatcgcc tttcgcaaca ttccgaatta
    44821 aatctttaag ttgtctagct gttttaatca ttaaaacaat acccccattg tttcacgaat
    44881 tctcttttcc agtctcatta tttttgcata ttccataagc cggttaaggt cttttccctt
    44941 ttgtttcatg tattcagtta aagcatagtt aaaagcggaa atatccatgt aagatcgttt
    45001 acggattaca tcacaaatag tgcgttccat atcgtatgca gtaatatcat atcctgcagg
    45061 cgaggacatt tgacagatac caagcgaata ccattccttc tttacataaa caatttttac
    45121 acccttttcc gttaatgtcg gattgttata ctttgcaggc accgttacag taagcggaat
    45181 cggttctttt tcggaaagat tgtgcaaata cagtgcagtt tcatgggaat atatgacttt
    45241 cggaatctga gtttgcagta agaacatttc gtctaccggc acatccggtg aaatataaat
    45301 gccgtgggca gctttttcaa gctctgtatc ttttgcgtaa ttatagaaag tttctttgca
    45361 aatacctgcg gcaacaatat cagccgtttt caaaatacca tcgttatccg ccaagatttt
    45421 gtcaatatag tcatacttca ttaaaaatca ccaactttca tacggatatt gtatcctaaa
    45481 gaccgtataa aagtcaatgc attatcggat tttctttctt ctaaataagt gaacggtata
    45541 accgttcgga tataccgtcc attgtaaatc tatatggtgg agataaacgg gatcgaaccg
    45601 ttggcctctt gaatgccatt caagcgctct cccagctgag ctataccccc gactttcaat
    45661 aatatactat cagttgtgcg gtaatgtcaa ccgttattta catatccggt gatgcatcgg
    45721 cattgatgtc aaagtcttct ttgttgatat agtatgctgc cgacgcaatc attgccgcat
    45781 tatcggtgca aagatgaata tcgggataga agacttccgt attctcttta gcggctcttt
    45841 tattcaatgc ttcccttaat cctttgtttg ccgccacacc cccggccacc actatttttt
    45901 tacttttagt ttcaaataca ttatccacca atacatcggt aaccgccttt tgaaaagatg
    45961 cggcgacatc ttccttggaa tagtcctcgt ttttctgttc ttttgaatgt atgtagttta
    46021 taacagcggt cttgagaccg gaaaaactga agtcaaacac attgtctctg aacttggttc
    46081 tgggaaaaga aattgcttcg ggattacctt ctttagccag tttgtctatt ttcggaccgc
    46141 cgggataaac cagccccatt acccttccga ccttatcgta aacctctcct accgcatcgt
    46201 cccgtgttcc gcctaaaatc ttaatatcat catacccttg tatatccaat atattggtat
    46261 gtccgcccga tatcagtaag catacaaaag gaggcttaag ctctttatgt gtcagataat
    46321 ttgctgctat atgacctttg agatgattaa ccggtattaa aggaatattt aaagatgcgg
    46381 caagtgcttt ggcagcagtt acacccacca acagagctcc tataagcccg ggtccgtatg
    46441 taacagctat tgcatctata tcttccctct ttatacctgc ttctttgatt gctctatcaa
    46501 tgacaggcag tatgtattcc acatgctttc ttgatgctat ttccggtacc actccgccgt
    46561 attcttcaaa tatctttgca gaggaagaaa taatattcga cagtataatt cttccgtctt
    46621 ttactacgga agcggcggtt tcgtcacagc ttgtttctat tcctaaaata atcataacac
    46681 tatatgaaat atgaactggt ccaatataca gaaaatgcct aaagcggcaa catagtatgc
    46741 aaagtagagt attttacctt ttaaaaccgt tttaacaaaa agcttaatgg caaagaatcc
    46801 cgatactcct gcaaatatca tgcctacaat aatcggcaac acttcgatat ttcccaatcc
    46861 gaccttaata gcaccatata tttccagaat aaatccgccc agtattacag gtattgaaag
    46921 taaaaaagaa aatttaacag catcttcttt tttaagtttc ctgaataatc cgcccgtaag
    46981 agtggagccc gacctggaga ttcccggtac aatcgccacc atctgtataa ccccgataat
    47041 cagggcatca taccatttca tattttcttt ctctaccgtt ccgggtttta atacggagga
    47101 cactatcagt aaaaccgcgg ttattaaaaa ccctataccc agagtagccc cgctgtcatt
    47161 taatccttca aaaaagtcgt taaacagaac acccgccaaa gccgcaggaa ttgtagctat
    47221 tatcagccat aatgtgagtt tctgaacagg tttcttaacc aactcaataa tatctttcca
    47281 aaacacgaca cagaccgcta ccaatgttgc aaaatgcacg gctatattaa atgtaagagt
    47341 gggtgtttct atacccagta tcttttcaaa cagcactaaa tgaccggaac tggataccgg
    47401 aagaaattcc gttaaaccct gaattattcc taatagaatt gcttgccaga tagtcatgac
    47461 acattttctt ccaaaagatt acctttatac actaatttgg gaggtgtatt gttcaatatg
    47521 gtactttcct ccactataca ctttacaatg gacgggtccg acggaaccga atacattact
    47581 tccataagtg ccgattctat aatggaccgc aatccgcgcg ctccgctctt tcgctccaaa
    47641 gtcttcttag ctatggcacg taatgccttt tcttcgaata ccagctcgca accgtccatt
    47701 tccaatatct ttttatactg ttttaccaag gcatttttag gttccttcag tatagaaaca
    47761 agaatatctt cagtaagctg ttccagcgta acgataaccg gcaatctgcc tacgaattcg
    47821 ggaataagtc cgtatttcaa caagtcctgc gtttcaatat gtttcaaaat cctgtctttt
    47881 tccacactgt ttgttttatg aatttccgct ccgaaaccta ttgctctttt acccaatctg
    47941 ttcaatatta tctcatctat accctggaat gctccgccgc atatgaacag tatattggta
    48001 gtatctatct gtatatattc ctgtgtcgga tgcttccttc ctccctgagg aggtacattg
    48061 gctacagtcc cttccagtat cttcaataat gcctgctgta ctccctcacc agaaacatct
    48121 ctggttattg aagggttatc gcctcttctg gctattttgt ccacttcgtc tatgtaaaca
    48181 attccccttt gagctctttg caggttatag tccgcggcct gtataagtct taataatatg
    48241 ttttccacat catctcccac ataaccggct tcggtaagcg ttgtagcatc ggagatggct
    48301 atggggacat tgagtatctt ggcaagagtc tgtgccatgt aggttttgcc cgtacctgta
    48361 ggacccaaca taagtatgtt gcttttttga atttccacat catcaggctc atgagcaaca
    48421 ttgtaactta tccttttata gtgattgtat accgctacgg atatggcctt ttttgcatca
    48481 tcctgcccta tcagataggt atccagttgt cttttaatct cttcgggatg aggaacttca
    48541 tctataaccg tttccgttac ctcttcctct tcatcatcat atcccagttc agtatcaaga
    48601 agatcggaac ataaatccac acactcattg catatataga caccgggacc ggagataagt
    48661 ctctgaaccc tgtcttcgga tttaccgcaa aaagagcatc tgatcttctt tatttcgtct
    48721 gacattcaat tctcctatct cttattcagt acttcatcaa ttatgccgta ttgttttgca
    48781 tcggctgatg acatgaagta gtccctttca acatcctttg ctatctgttc taagggttgc
    48841 ccggtttttt ccgccaatat cctgttgaga ttatccttta ctttcaacag ccaatcggta
    48901 tgaaggcgta tatcggttgc ctgcccctgt attcctccgg acggctgatg tatcattatc
    48961 tggctgttgg gcagagcata tctcttccct ttggtacctg ccgcaagcag aaacgctccc
    49021 atggaagctg ccaatcctac acacattgtg gaaacatcgc atttaacaag ttgcatggta
    49081 tcgtaaatcg ccaatcccga tgtaaccgat ccgcccgggc tgttaatgta cagctttata
    49141 tctttttccg gatcttcatg ttctaagaac agcatctggg ctattataag gcttgctgca
    49201 atatcattga cctcacttcc caagaatact atcctgtcct tcagcagtct ggaaaaaatg
    49261 tcatatgacc tctctcctct gctggtctgt tcaattacat atggtatggt attcatcact
    49321 tatccttcct tgtcggctat tacttttccg acttatcttc cgcggtcttc ttgggagccg
    49381 ctttcttttc gggttcctcg tcctgtttct tttttttatc tacgctcttt ttctctttaa
    49441 cttctttatc ttttttatct tctttaactt ctttaacttt ctttgctgag gaatatatta
    49501 aatccacaac tttatcgctt gctattttat cggtatagta ttgcttataa tcgcctatgg
    49561 cttgctttat ccggtccggt tccatcttgt atgtttccgc atctttatta agcagctctt
    49621 caatttcttc gtcggtaacc tgaatgtttt caacattctt aatcgcttcc actaccagat
    49681 cgcgaactac catcattcct gcctgttcct tatactgact gcggatatct tccatcttca
    49741 ttccggtata ttccagatat tgctccaacg tatatccgct gtattgcatt tccatcctca
    49801 tattgtcaat aatattgtcg gtcgcccttt ctatcatggc atttggtata tcaaccttag
    49861 catttttgca tgcggttgca agtgctgccg aacgcatgga atcttccgct cttttctgtc
    49921 tttcttccag cattttcttt tttgtttctt ccttatactg ctcaaccgta tcgtattcgc
    49981 ttatgtcttt tataaactca tcatcaataa ccggcaattc cttgactttt atctcatgaa
    50041 gtactacctt gaaaacagct tgcttgcctt ttaattcctc cgcatgatac tcatcgggaa
    50101 acttgacatt gacatcaaac tcatcattaa tgttgtgccc gataatctgc tcttcaaatc
    50161 ccggtataaa tgtttttgaa cctattacca actgttgttt ttccgccgtt ccgccttcaa
    50221 aagcaacatt gtcaacaaat ccttcgtaat caatgattac ggtatctcca tcctgtacgg
    50281 gacgatcttc aataccgatc atacgggagt tcttatctgc ttctctgttg agggcatctt
    50341 caacatcttt atcacttact tcaacttccg ctttctttat ctcaacacct ttgtatttac
    50401 ccaattccac atcgggtttg acatccacgt taatagagaa ggtaatgccg ttttcttcat
    50461 ccatatcttt taaatccaca tgcggataat ccactacatc caatttgaga ttatctattg
    50521 ctttgggata ttccgcattc aaagccatat ccaccgctgt atcataaaaa atacctttcc
    50581 cataaattct ttcaatcaaa tactgaggag ctttcccctt cctgaaaccc tgtatcgtat
    50641 acttattctt gtctttgtta tatgcctttg taatatatcc gtcaaattcc tcttttgtaa
    50701 gagcaatctc catttccact ttatagcctt ctttttttac tattttgctt tccattacat
    50761 acctccgcct tctttgcgtc gcagtttata ataacatatg gtacttgtta aatcaataaa
    50821 attaaacaaa taccctgaat tattacatat tatgtctgca cattcctaca caagcagact
    50881 gtcccgtttc atatacttta ttcgaatctg aaaaagcttt ttgctataga caaacacaac
    50941 actgcacttt tgtttcggtg cagtgttgtt aatctgtttg aaatgtattt tagttataca
    51001 gtctttctta tctccagctt gtattttccg gactccagct ccagatattc tttacctaag
    51061 agattgttgt cttcatatat ctccatacct gctcccgttt gaagaaccgc acttgtattg
    51121 gtaggaatct caatctccat ggatattact ttaccctctt tgacgtatga tgaacgtatt
    51181 gttccgtaca tactttcaaa aaccgcatct acatttacca gcggagtatt aaatgccgga
    51241 gcaatggtaa atttcttgaa acccggatag tcgggatcgg gccttatgcc tgcaaaatat
    51301 ttgaagaatg tagcggatat gtcaccgaac atgtgatgat ttctggaacc ttctccgttc
    51361 catgtctcat agagagttgt tgcacctaat tctttaattt catgtgcata actcgggaac
    51421 gtatctttag taatcatgga atatatcaaa tcggcacggc cttgtcttcc taatatattg
    51481 ttcacatact tattacccag tatgccgtaa tccagatgtc cgtcggcctc gttaatagtt
    51541 cttatgagat gactgtaaat aaggtcgtat tccttattac cttcataaag gttatgataa
    51601 agcattgcgg cataacaggt ctgtccgtct cctttaagcc tgcctgtctg agggtcataa
    51661 aaagtttttc tgaaacctgc tttaatcttc tttgcggttt tattgaattc ctttgcatcg
    51721 tcacggtgtc ctaacaattt tgcaaatttc tccagtacat tggccatggt atacatacat
    51781 gccgtatcgg ttaaatcgat aggtgcttta aatgacgaca tattgactga aatagccggt
    51841 ccttcaaaag gtgcacacca gtcgcccacc ccgtagtcat tgacgcatac atcgttctgc
    51901 atcatggaaa gcatgtattt catgtttctc ttcatgtact gatagtattt gcgtattaca
    51961 tatttatccg cgtaatatgt atacatactg tcgggaagcc atgtcagtac ggaagagtag
    52021 tccggtccgt tcccccagtt atatccccat cctccggtcg gtaccataca gggtatattt
    52081 ccctttgcct tcatggaatc tttaatatcg ttaagccact tcatccacaa gggaccggac
    52141 atatagttga agagaatctg ctcggtggac acattggtat ctcctgtcca tgcgttcttc
    52201 tctctgtgag gcgaatcggt caataaaccg tacatgttgg aataggtggc cattctggta
    52261 ttcttctgaa tctgatttat aatgtcgtcg gaacaggaga actgtcctat ttcctttact
    52321 gcggtatgaa ggaatatagc ggttgcactc gacaaatccg gctggcttgt aaatccttct
    52381 atttgtgcat atctgaaacc gtggtatacg aatctgggtt gccaggtttc ctctccgtca
    52441 ccctttttaa tatacttatc cgtctggaat tcacccgtct ttgtgaaacc gctgttatag
    52501 acagtatcca atgtgccgtc atctttaagt ttttcattca tctttatgac aaactcggtg
    52561 cctttgggac ctgttacttt tattctgaca aatcccgcca tgttctgtcc gaaatcaaac
    52621 agatatgtgt ttttggctac attcttaaca ctgattgccg gatattctct gtcaactttc
    52681 accgaaggca tttcatttgc tttgagtaat cctcccggat cgcgtgatat acttacattt
    52741 acccaatcat caactaatgc atcaggttcg caccaggcag gtatttccaa cctcgcatca
    52801 tagaactctc cgtttcttaa actgttaaag gtaatgggac tcatatggct ttcccacgta
    52861 atatccgaag gaatacatat ctgcttatta ttcttggtgg tgatatacag ttcgcatatc
    52921 atcttaggac tgcccttcca ggaagcggca tgggtattcc actgatcaat tgtaggataa
    52981 ttgaagaatc cgtttcctaa aataacaccc agcatattgg agcccttacg taagtattcc
    53041 gttacattga aagtgtagta cagaagagtt ctgtcatatg cggtaaaagg cggatccata
    53101 atatggtcat ttatcttttt accgttaata tagggttcaa agaaacctgc tccggttata
    53161 tagagcatgg ctttggaaaa atcttctgta atatcaaacc ttttccttaa gtaaggagaa
    53221 gaagccgcaa gttctctggg cactctgtat cctgcttgta tatatttgcc gacccactct
    53281 tcttccagtt tacccgtatc aaaaaaagaa tcttcacttt gtgctctttc accttttttg
    53341 tcggttacac ttacattcca gcgatatgtg gtcctcggca gcagtttctt acctttatac
    53401 tcgataagta cggattcatt gcttctgaca tttcgcgaat cccataccac cttgctcccc
    53461 gtcatgacct tgattctgta tgaacgttgt ttgttacctc ttccgtcctg tgcaagcttc
    53521 catgagaatc tcggaatctc atcaattccc aacgggttgg taagatagtt tactgttaaa
    53581 tccttgatag tgatagacat ttactcatct cctgacttac ttattttgcc ttttaattat
    53641 atctttttta taactgcatg acaaggtagc aaaagtcccg gcaaactcaa aagaaaataa
    53701 aagaaagaat acgttttaat tcaaaattgc tttaaaaaaa acggaagcaa gtgtaaacct
    53761 gcttccatag tgtaaactat tgctatagtc ttacttcatt atcttaacaa cgcttcctgc
    53821 gcctactgtc tttccgcctt ctcttatagc gaatctcaag ccttcgtcca tagcgatagg
    53881 tgtgataaga gcaattctca tatcaatgtg gtcgccgggc ataaccattt ccacaccttc
    53941 gggaaggttg gcaacaccgg taacgtcggt tgttctgaaa tagaactgag gtctgtaacc
    54001 tgtgaagaac ggtgtatgtc ttccgccttc ttccgctgtc agcacgtata cctgtccgac
    54061 aaaatcggta tgaggtttga tggagccggg ttttgcaaga acctgtcctc tttcgatatc
    54121 ggttctctga actcctctta acagacatcc tatattgtca cctgcctgtg caaaatccag
    54181 aatctttctg aacatttcta cacctgtaac aactgtagat gcaatctttt ccgtaagtcc
    54241 tactatctct accgtatctt gaattttaac agttcctctt tctactcttc ctgttgcaac
    54301 agttcctctt cccgtaattg agaaaacgtc ctcaaccggc attaagaaag gcttatcaac
    54361 atctctttgc ggagtaggaa tatatgagtc aaccgtttcc ataagtttaa ggattgatgc
    54421 atactccggt gcattcggat ctgtggaagt tgattgtaaa gcctgcaatg cggaacccat
    54481 gactatggga atatcatctc cggggaactc atactcgtta agtatttctc ttaattccat
    54541 ctcaacaagt tccagaatct cgggatcgtc aacctggtcg gttttattca tatatactac
    54601 aatataagga actccaacct gacgggcaag cagtatatgt tcttttgtct gaggcatcgg
    54661 gccgtcagca gcggaaacaa ccagaatagc tccgtccatc tgtgctgcac ctgtaatcat
    54721 gttcttaaca tagtctgcgt gacccgggca gtcaacgtgt gcatagtgtc tgttctttgt
    54781 ttcatactca acatgagctg tagcaatggt gattcctctt gctttttctt caggtgctgc
    54841 gtcaatctgc tcgtatgctt tgtaaacgat atttttatca cttaaagcca atgtctttgt
    54901 aatagcagca gttaatgtgg tcttgccatg gtctacgtgt ccgatagtac cgatgttgca
    54961 atgtggttta gttctttcaa attttgcttt tgccatttta ttatttcctc caattataat
    55021 gagctgattt ctatattaat ttatatacat tctacatatg aatgcgggtt gtgtcaatag
    55081 ttcgttttga gtatttttgc cgctaaatcg tcaggtatcc tctcaaaatg gtcgtattgc
    55141 atggtaaatg tgcctcttcc ttgcgtattt gatctaagat cggtagcata tccgaacatc
    55201 tccttcaaag gcacgattgc ggtgattacc gattcagttt ttccggtttc cacattctct
    55261 atttttcctc tttttgccgt tatgtcgccc aacacatctc ccaaacagtt ttcgggaaca
    55321 aatatgtcta cacgcattac cggctctaaa agaatgggat cggccttcat acatgcttct
    55381 ctgaaaccca tgcttcccgc tatcatgaaa gccatatccg aggagtccac ctcatggtag
    55441 gaaccgtcca gaagggttac ggtaacatct acaatcggat atccagcctg aataccgttg
    55501 ctcaaggatt ctcttatgcc tttttccacc gccggaatat atattttggg tataactcct
    55561 cctacggttt tatcgataaa ggtaaaccct tcgcccggtt tagaaggtgc tacttccagt
    55621 actacatgac cgtactgtcc gtgccctccc gtttgcctta taaactttcc ttctgcacgt
    55681 gcaggttttg tgatggtttc cttgtaagtt acctgaggct ttcctttggt tgcttctacg
    55741 gaaaactctc ttctcattct atccacaata atatccagat gaagctctcc cattcccgat
    55801 atgagaacct gtccggtatc tttgtcggtt ttgaatttga aggtcggatc ctcttcggaa
    55861 agtttctgta aagccaatgc cattctgtcc tgattgattt tatctttgcc ttctacggct
    55921 atggatataa ccggttcggg aaactgcatt gattccaaaa ttacaggatc tttggggtca
    55981 caaagagtat ctcccgtcac agtgaacttt aagcctaccg cacaggcaat atctccggaa
    56041 taaactttac tgatatcggt cctgctgttg gagtgcatca tgagtattct tccgatacgc
    56101 tcttttttat ccttggtcgt attcagtaca taggaacctg tttccagtgt cccggaatat
    56161 acccggaaaa agcatatttt accgacaaac ggatcggaca ttattttgaa tgccagtgcg
    56221 gcaaacggtt catcatcccg tgacggtttg gttatcactt tggaatggtc atgcatgctg
    56281 acaccttcta tgtcactgac atccaccggc gacggaaggt aatcggtaat agcatccaga
    56341 agtttctgaa cccctttatt tttgtatgaa gatccgcaca atactggagt tatggttaag
    56401 ttgacggttc ccgaccttaa agctgctttt atttcgcttt cctccacatc ctctccctca
    56461 agatacttct ccataaggca ttccgattca tcacataccg attcaatgag tttaatccta
    56521 tactcggtag ccttatccaa catatcatca ggtatatcca gttcctcaat actattgccc
    56581 aaatcatccc tgtagaaata tgctttgaac ttaacaaggt ctattattcc cgtaaagtcc
    56641 gactctttac ctataggtaa ctggatggga accggattgg cattgagtcg cttcctcatc
    56701 atttccacac aacggaaaaa gtccgcaccg gtagtatcca ttttattgat ataggcaatt
    56761 ctgggtacct tatacctgtc cgcctgtctc cataccgttt ccgactgagg ttccacaccg
    56821 ccttttgcac agaaaaccga aacggcaccg tcaagcaccc taagacttct ctccacctcg
    56881 gcggtaaaat ccacatgacc gggagtatcg ataatgttca gtcggtagtc tttccaatag
    56941 gctgtcgtgg cagcggaagt tatggtaata ccacgctcct gctcctgctc catccagtcc
    57001 attgttgccg ctccctcatg aacctctccc atcctgtgaa gctttccggt gtagtagaga
    57061 attctttcgg tggttgtggt tttcccggca tcaatatgag ccattattcc gatatttctg
    57121 gtaaattcca gtgcataatc tctcggcata ttattctttc cgaattacca cctgtaatga
    57181 gcaaacgcct tgttggcctc tgccattctg tgcatttctt cttttctctt gaaggtattg
    57241 ctgactccgt tgacggaatc cataatctca gcagcaagtc gctgtttcat agtgtgttca
    57301 ttgcggtttc ttgcaaactg cacaagccat ctcaaagcaa gagcctgtct tctctcggga
    57361 cgaacttcca caggaacctg atatgttgcg cctcctactc ttcttgcctt aacttcaaga
    57421 gcaggcatta tattctccaa agcctgttta aagacttcca gaggatcttt gcccgtcttt
    57481 gttttgataa tatcaaaagc atcatagcat attctctgtg ctacgccttt tttaccatca
    57541 agcattatat tgtttattaa ttttgccacc accttgtcct tatataccgg atcgggcagt
    57601 atttctcttt ttgggacatg tccttttctt ggcacttgtc ttccctcctt aataataatt
    57661 tttcataggt actcagccgt gtcagctgtc agtccgggta catatcacct tttaatatat
    57721 gttaagtacc ggatatttct aagtacgtta tttacctttt tttgcaccgt atttggatct
    57781 gccttgtttt ctgttagcaa ctccctgggt gtccaatgtt cctctgataa tgtgatacct
    57841 tacgccgggc aagtctttga ctcttccgcc tcttatgaga acaacactgt gttcctgaag
    57901 attatgtcct attcccggta tatatgctgt cacttcataa ccgttggata gtcttactct
    57961 tgcgattttc ctcaatgctg aattaggctt cttgggagtg ttggttttta ccaccgtaca
    58021 gactcctctc ttttgaggac aagctactga cgtagttctc ttccttatgg tattcatgct
    58081 tttctgcaaa gccggcgaca gtgatttctt tttggaaacc tgtcttcctt ttgttaccag
    58141 ttgattaaat gtcggcatct tttccctcct ttctttaagt agttatatat ttacttcaaa
    58201 atacataccg ctgatgcttt cacacttata ccgtttaaca atcccaattg tttggcggta
    58261 tcggcatatc ttatctcaat accttttttc tcacataatt ctgcaagcgg ctcaatcagt
    58321 gagcggtcgg catctcttgc aatgtatacc gtcattgccc ggtcttctaa aacggcttta
    58381 gccgattgtt taaggcctat caccttttta tcctgcgacg ctgccttaat cattattccg
    58441 tctgctcttc ttccgattcg gatatgtcgt cggaatcggc atcttccgaa aggacttcat
    58501 catcatactc ttcggaatcg tcttggtcaa gagtttcttc atcatcattc atctcgtcaa
    58561 acaaatccac ttcatcttca tagccttctt cggcaatgaa atcctcttca tcatctatcg
    58621 gatccatctc aagatacggg tcctgataag cattcatgcc tgttccggcc ggaataagct
    58681 ttccgataat aacattctcc ttaagtccta tcaggttatc ggttttgcct ttgatggaag
    58741 cttcggtaag aacacgtgtt gtttcctgga aggaagcggc ggaaaggaat gaatcggtgg
    58801 caagagaagc tttactgata cctaacaata ctacttcccc cgtagcaggc tgtttgcctt
    58861 cctgttcggc aagtttattc tgtttataga aatcagttgc atcgcagtaa cttccgggta
    58921 tgagattggt gtctccgcaa tcttccactt ttactttacg caacatctgc ttaactatta
    58981 tttctatatg cttatcgtta atatcaatac cctgaagttt gtaaactttc tgtacttcgc
    59041 aggcaatata tctgtgtact ccttctacac cttttatcct taagatatca tgcggatttt
    59101 ccgaaccttc ggttatctta tcgccttttt tgatctcctg gtcatcgaca acggtcagtc
    59161 tggaaccgta aggaatagtg tatgtatgag actcatcctc acccgtaaca ataacttccc
    59221 tgcgttttct gctctcattt atcttaacaa taccgtcatt ttccgatata attgccagcc
    59281 ctttgggttt tctggcttca aaaagttcct cgacacgcgg cagaccttga gtaatatcgt
    59341 ctcccgcaat accgcccgta tggaaggttc tcatggtaag ctgagtaccg ggctcaccta
    59401 tcgactgagc ggcaataata ccgacagctt ccccgatatt aacgggcttg ctggaagcaa
    59461 ggttcattcc gtagcatttt gcacaaactc cgagttccga cttacaggtt aataccgaac
    59521 gcaccttaac ttctttaata cccgcatcaa caattctctt ggtagcatcg gcatctatat
    59581 atccgccggc ttccactatg atttcgcctg ttttcttatc tacaacggga tcggtgacat
    59641 aacgaccgta tatcctgtct ttcagtttta ctacaacttc accgctgtcc acaatatcgg
    59701 aaacatagat accgtcttcc gttccgcaat cggcttctct tactattaca tcctgcgaaa
    59761 cgtccacaag tcttcttgta agataaccgg agtcggccgt tcttaacgcc gtatcggcaa
    59821 gacctttcct tgcaccgtta ctggaaatga agaattccag aacattcaag ccttcacgga
    59881 agttggactt tatcggaatc tctatggtct ttccggctgt atcaaacatc agtcctctca
    59941 tacccgcaag ttgctttatc tggttcatat cgcctctggc tcccgatctg ctcattacat
    60001 taagaggatt gaatgcatcc aagccttcca taagcatctt tgtaacgtca tcggttgttt
    60061 ttctccaaag ctttattatc tgatcgtgtc tctcttcatc ggttatgaga cctcttctga
    60121 agtcatgggt aattttatcc acctttttct cggtagcttc aagaatctcg ttcttggagc
    60181 tggggattat catgtcgctt accgatatgg aaatagcacc ctgagtggag tatctgaatc
    60241 cgatggattt tatcttatcc agcacttcgg cggtttttgc aataccgtgt ttggttatgc
    60301 atctttcaat gatttccgaa agattctttt tacccaccat ctgatctatt tccagattaa
    60361 acatggtatc gggattggtc ctgtccacaa aacccaggtc ctgaggaata gcttcattga
    60421 atataacttt accgcaagtt acatcaatta ttttgcttac aacttttccg tctacttctc
    60481 tttccgttct cattttaacc ttggaatgaa gagttattgt cttttcatgc aatgccataa
    60541 gcatttcgtc aaagttgcgg aatactcttc cttcgcctat ctctccgtct tttgcggtgg
    60601 taagatagta acctcccaga accatatcct gagtgggaac ggtaatgggt tttccgtctt
    60661 tgggtgcaag cagattgttt gcggaaagca taagtattct ggcttccgac tgagcctctg
    60721 cagaaagagg aacgtgaaca gccatctggt ctccgtcaaa gtccgcattg tatgcggtac
    60781 ataccaacgg atgcagcttt attgctcttc cctctacaag aaccggttca aacgcctgta
    60841 ttcccaatct gtgcagagta ggtgctctgt ttagaagaac ggggtgatct ttaataacat
    60901 cctcaagaat atcccatact tcattgcgta ctctttccac cattttcttt gcactcttaa
    60961 tattctgagc gaacttatcg gtaacaagac gtttcatgac aaagggcttg aaaagctcca
    61021 gtgccatttc cttgggaaga ccgcattgat acatcatcag ttccggtccc acaactataa
    61081 ccgaacgtcc ggaatagtct actctcttac ccaaaaggtt ctgtctgaat cggccctgtt
    61141 ttcctcttaa catatccgat aaggatttta gcggtctgtt gcccggtcct accacgggtt
    61201 ttccttttct gccgttgttt atgagggcat cgacagcttc ctgaagcatc cttttttcat
    61261 ttctgactat aatatcggga gcggtaagct ccaaaagcct cttaagcctg ttatttctgt
    61321 taataactct tctgtaaagg tcattaaggt cggaagtggc aaatctgccg ccgtccaact
    61381 gcaccatggg gcgaagatcg ggaggtataa cgggaatggt atccaaaatc atccattccg
    61441 gcttgtttcc ggacactctg aatgattcca tagcttccaa tctcttgatt attcttcctc
    61501 ttttttgacc tgtgctcgtc tgcagttctt ctttaagaag agcactctcc tcttcaacat
    61561 caattcttgc aagtaatatc tgtatcgctt cggcacccat acgtgctttg aaagagtctc
    61621 tgccgtactt gtcgattaat tctctgtact gaagttccgt aagtaaatct tttacttcaa
    61681 tggttttgtc gttaaccttc acatcctcat tattcacttc cagcactaca taagagttaa
    61741 aatacagaat cctttccaat gctctgggac tcatatccag tacaagaccc attctgctgg
    61801 gaattcctct gaaataccat atgtgggaga caggcgcggc aagttctata tgtcccatac
    61861 gctctcttct taccttgctt ttggtaattt ccactccgca acggtcacat atgatgcctt
    61921 tgtgtctgac tcttttatac tttccgcaat ggcattccca gtccttggtg ggaccgaata
    61981 ttttttcaca gaagagtccg ccggcttcag gtttcagtgt tctgtagtta attgtttcag
    62041 gtttcttaac ctcgcctctg gaccattcct taatcatctc ggaagaagcc aaacctatct
    62101 taatcatgtc aaaatttgta taatcttgca ttctttactc tcccatgtca tctaaatcaa
    62161 ctatatcctg cacatcggat atctttgaat aatctacgcc ggtgtcatcg tcatcagcgg
    62221 ttttcatgct cataccgggc atttcctcgg aatcgttatc ttggtcttca gaatcgtcgt
    62281 caacttttgc cagctccata ttgaataatt catcattatc cagcgtgtaa tcttcggttc
    62341 cggaaatatt gatatccaat ctgggtatat catcttcact tccgtcacga agcataactt
    62401 ccgaatggtc cgacttgagt atcttcacgt ccaaagcaag actctgaagt tccttaacca
    62461 gaaccttaaa ggattccgga ataccggatt tggggatact ttctccctta acaattgcct
    62521 cgtatgtctt tacccttccg acaacatcat cggatttgac ggtcaatatc tcctgcaaag
    62581 tataggcggc gccgtatgct tctaatgccc acacttccat ttctccgaaa cgctgtccgc
    62641 cgaactgtgc tttaccgccc aaaggctgtt gcgtaaccaa cgagtacggt cctgtggaac
    62701 gggcatgaat cttatcatca acaaggtgat gcagtttaag catatacata tagcctacgg
    62761 taactctgtt gtcaaaaggc tctcccgttc ttccgtcata cagaacggtt tttccgtctt
    62821 ccgacaggtt tgccatctta agacactcga ttatgtccat ttccgatgcg ccgtcgaata
    62881 caggtgtggc tattttccat cccaaagctt ttgcggcata tcctaaatgc acttccagta
    62941 cctgcccgat gttcatacgc gaaggaacac ctaaaggatt caatactatc tgcaacggtg
    63001 tgccgtcttc taagaaaggc atatccgctt cgggtaacac acgggagata acacccttgt
    63061 taccgtgacg tccggccatc ttatcaccga cggctatctt tctcttctga gcgataaaca
    63121 ctcttacaac ctgattaact ccgggattga ggtcgtcgcc ggcttctttt gtaaacagtt
    63181 taacatccac tactataccc gattcaccgt gaggtactct cagcgatgta tctcttacat
    63241 ctctggattt ttctccgaat atggctctca aaagtcgctc ttccggtgta agctccgttt
    63301 ctcccttggg agttacttta cctacaagta tatcgcctgc ctgaacttcg gcaccgatac
    63361 gtataatacc tctttcatcc aggtctttaa gtgcatcatc accgatattg ggtatatctc
    63421 ttgttatatc ctcaggcccg agtttggtat ctcttgcttc cagatcatat tcttcaatgt
    63481 gaatggatgt gaaaacatcg tcctcgacca gtcgctcact gataagtatg gcatcctcgt
    63541 agttatagcc ttcccatgtc ataaatccga ccagcacgtt ctttcctaag gaaatttctc
    63601 ctccgtctgt ggaaggcccg tcggcaatga catcgcctgc ttcaacctta tcgcctttct
    63661 tgacaataac tttctggttt atgcatgttc cctgattact tcttttatat ttaagtatct
    63721 tatattcgtc ccgctcaccg tccacggtat caataaccac cctgtcggct gaaacatttt
    63781 tgacttctcc cgctcttttt gccaatacgc atactcccga gtcggtagca accttatatt
    63841 ccatgccggt agcaataatg ggagcttcgg ttcttatgag gggaacggcc tgtctttgca
    63901 tgttggaacc catcagcgct ctgttggcat cgtcattttc caaaaaaggt atcatgcttg
    63961 tggcaaccga taccagctgt ttgggagata cgtccatata gtcaacatcc ttggaatctc
    64021 tcattacctt gttattgcga tatctgcagc tcactctggg cgttgcgaaa cgaccgtctg
    64081 gggtcagttc cgagttggcc tgagccacaa tataaatgtc ctcttcatct gctgtcaggt
    64141 aatcaatttc ttcggtaacc gtgtgagttt ctttatttac tctgcgatac ggtgtttcga
    64201 taaatccgta ttcattaatt cttgcatatg tactcaaaga agctataagt cctatgttgg
    64261 gtccttccgg agtctcaatg gggcacattc ttccgtaatg cgagtagtga acgtcacgaa
    64321 cctcgaagtt ggcccgctct ctgctcaaac cgccggggcc taaagccgaa agtcttctct
    64381 tatgggttat ctcggcaaga ggattgggct gatccataaa ctgtgacagc tggctggagc
    64441 cgaaaaactc ctttattacc gccgttacgg gacggatgtt aatcaatgcc tgaggtgtta
    64501 caatatcaat gtcctgaatg gtcatcttct cacggattac ccgctccatt cttgcaaacc
    64561 ctatcctgaa ctgattctgc agcaattctc ctaccgatct tattcttctg ttgcccagat
    64621 ggtctatgtc gtcggtgtgg tacagaccgt aatccaatcc cagcaagtaa cttatggaag
    64681 ctataatatc aatcctggta atgtttttag gacataagtc atatcttctt ttttcaaaag
    64741 cttcttttat ttcttcattt gtggaacatt cggcaagtat ttctttcagt actttgtagt
    64801 ttaccttatc ggtaagcccc agtgcggtat gattgaaatc cacaaagtgt gaggcatcaa
    64861 ccgtctcatt tcctataact acatgaacat gattatcaca ttttactttt accacgttta
    64921 ttccggcatt ttccaccgca attgccttag cctcgtcaat aacttcatcg gcttccacca
    64981 atatctctcc tgtctcggca tctattattc tctcggctga tacagttcct gctattctcg
    65041 gagcaacgga aagcttcttg ttgaatttat atcttccgaa tctggcaaga tcatatcttc
    65101 tggcatcaaa gaacaatcct tcaaataagt tttcggcact ttctatcgta ggaggctcgc
    65161 ccggtcttaa ccttttgtat atctcaaaca atccgccttc tctggtcttt gtttcgtctt
    65221 ttgcatatgt tgcatccagt ttatagtctt ctccgaatac cgcggttata tcggaatctt
    65281 tggcaaatcc caaagctctt aagaaaaccg tcagaggtaa ctttctggtt ctgtctattc
    65341 ttacatacaa aaccccgttg gaatcggtct catattctat ccatgctccc ctgttgggta
    65401 taagtgtgct ggaaaaaaga ctttttccgg ctttatcctt cttctccgca taatatgcgc
    65461 ctggagatct taccagctgg ctgacaataa cccgctccgc accgttaatg ataaatgtgc
    65521 cggtgtcggt cattatgggg aaatctccca taaatatttc ctgttccttg acctctccgg
    65581 tatcttttct tataagacgg acttttactc tcaaaggagc ctgataagtg gcatctcttt
    65641 ccttacattc ctgtacgtca agtttaggct tgtccgcaat cctgtaatca ataaaatcca
    65701 gcattaaatt atcggtatga tcacgaaccg gtgatacatc cttaaaaacc tctctgagtc
    65761 cctcatcgag gaaccactga taggactttt tctgaacttc tataagattg ggcatttcca
    65821 gaacttcttt gatctttgag aaactcattc tttctcgttt tccgtattga ataggatgta
    65881 tcattaatta gtcacctcat gtccttttct atatcatccc catggtaacg gctgccattt
    65941 tcagtgtcgg caaaccggtg ccataattac cttatgcacc tccgattgca gtaatttcct
    66001 atcgacaggc gcaaaaatac gcagattagc gcatcattca caatcttatc gcatacatcc
    66061 gcccgtgtca atagaaatat ataaatttta ttcttaatta atcgcttttt tctttagtcg
    66121 gctgctttaa tcttaaggta caatcagttg agatatgttt ccttaatctt tgtcatatat
    66181 tcttcaagat atttcttaag cccgggagaa gcatggtcca tcagcatgtt tattttttct
    66241 ccctgattat atatctcctc ggataaaatc tcctccatac gtttgttggt ctgtaaatcg
    66301 aatatccgta tattctttaa ggtagactct atttcctttt ctataagcac aagaagagcc
    66361 acatcccttt cgtccaaggc aagctcctcc aatatacctt cttcaaaacc cgtcatgtca
    66421 tcataatcag tttttttcat atatacctat atccatcgta ttcttatccg gttgtcttac
    66481 tatggtaaga atgtaatcat tattctcagc aacaaaaacc gtctccgaag tcaccagtat
    66541 tgcagtaaac cctgcagcta tcgcatccgc cgtgtatgac tcgaatttct ctttaccgat
    66601 atcctcggta actaccgtta tattgttgga ttcgcctata atctgtaaaa cctttgcgcc
    66661 gggaggtgcg gacaacattg tgatatatcc ttctttcggc catgtaagag gagtggtcag
    66721 ttcaataacc attttatgcg tatcgacatc cctgttcatt gccagataat agtcatccaa
    66781 cgatcccgca aaagcttctt ttgtttccac acttatattc ttatacccga cacccttcaa
    66841 tgtctctata tacttcataa aggattcatc gtccacgtta taaagataaa ttctcagtat
    66901 ctgaccgtaa aacttgtaat agttgacttc gccgccttta atcttaggca aaatatccgc
    66961 tcccagatat tccgaaggcc agttctgctc ggtcgttacg acataactcg gagtaccctc
    67021 gtcaggtgtc ttcttattat tgcaagcggt caatgtaaag accatgatta cgaccaatat
    67081 aagtgcagtg attcttcttg tcattctctc ctccgctaca tgcttttcct gtaatagttt
    67141 atcatacaac cttcggcaag tacatccctt tcatcatccg ataatccctc catatataac
    67201 ctgaccgctc ttgctttatc ggtcctttgc aaagcatctt cctttgttgc cgacacatat
    67261 gcaattacct gctctttccc cgaaagaacg gcgtctctta tgtcgggaat atagacatat
    67321 tcatctacgg taagagcggg atcctgtttt accgtaaaag gtataagtcc ccagtttatg
    67381 aggttggagc gatatctctt tgtggcatat cggtatgcga tattggcata tcctttcagt
    67441 actctctgac aggaagcagc ctgctcccgg gcggaaccgt ctcccggttt tttggcgaat
    67501 atgaggtgac cgtccacacc tgcttccctt acggcaagtt ttgttttatt tacataatcc
    67561 ggagctcttc tggacaatgt atattccgca agagccatgg gattggacct gtaagaggaa
    67621 gtctcccccg aaggtataag ttcgtcggtg gtggtaacct cgtcatttaa taccgcagct
    67681 actttcaata acaaattctt aggcaatctc ggtatttccg gccacacctt tatattggga
    67741 ccgtatatca gctcctgatt tttgtcaggt tttccgtatc cgttatatac tcttttttca
    67801 tatatgttac cgttaaattc atatacggga atatctttaa catattctat atcggtaccc
    67861 gcagttatat atcctttatt caatgcggta gcggcaatgc ttctggcatc catcagtgcg
    67921 acatacgcgg cctgaccgtc cttggggatg ctgccttcac gattctcgaa attccttgtg
    67981 gtatgcctta ccgacaggac attgttggca ggtatgtcac cggcaccgaa acacggtccg
    68041 cagaatgccg tttttataac aacacccgat tccataagct tgtttgtaat ccccgcctta
    68101 ttaagcgcat atgcaaccgg ctgactttga ggataaacgg acaaggagaa actgccactt
    68161 ccggtatcac ctttttgcaa aacagccgca gcttcacaga tattctcaaa agaacctccc
    68221 gcacatcccg ctattattcc ctgtcccgta aaaaagcgtc tcttcttact gtcggcatat
    68281 actttgagac tgccgagatt caactgttcc agtatgtctt cactattttc caaaaagtcc
    68341 ttaacggtat atgcattgga aggatgatac ggcaaagcta tcatgggttg tattttggaa
    68401 aggtcaatca ttaaggcaca gtcatacagt gcttcttttg ccggagaaag ctttttataa
    68461 tcttccttcc tcccgtgtat ctcatagtac tcttttgttt tatcgtcggt tacccatatg
    68521 gaagaaagac atgccgtttc ggtggtcata acatcaatac ctattctgtc atccatagaa
    68581 agattgttaa ctcccggtcc cgcaaactcc aatattttgt tggtaaccga agcatcctta
    68641 aatacttttc ctatcatgta tatggctaca tcgtggggtc ccactccctg attaagagta
    68701 cctgtaagat atacaagaca taccttggga tatgcaagat cataggtttt tcccaacagc
    68761 tgctttacca gttctcctcc gccctcaccc atggccaagg tgcccaacgc cccgtacctt
    68821 gtgtggctgt ccgaccctaa aatcatcttg ccgcaccccg ccattttctc ccgcatgtac
    68881 tgatgtatta ccgcaagatg agcgggtaca taaatgcctc cgtatttttt tgccgcggac
    68941 aaaccgaaaa gatggtcatc ttcattgatg gtaccgccta ccgcacaaag agagttatgg
    69001 caattggtca gaacataagg aatagggaat ttttctaaat tacatgcgga agcggtctgt
    69061 aaaatactta catatgtgat gtcatggctt gcaacagcat caaatttaat tttgagcata
    69121 tgatttgaag cggatgtatt gtgtgatttt aatatagaat gagcaatggt accctcgcgt
    69181 gctctttgta cctgttcttc agacagattc gctgctttca gcactttatc ttcctttaaa
    69241 actactgctt cggttataaa catcggaaaa tcatcctttc tatgttatac tttattatac
    69301 ttgaacggaa aggttatgca aatgcataaa tatgattgaa taatgaaata caagcatatt
    69361 atctgggatt ttgacggaac gctgtttgac acatacggag agattgcgaa agttatcgaa
    69421 gaggttctga aaaacagagg aatagttgag aatcttgatt ggatagtaaa aaacttacat
    69481 atatcgctta cacactctct gacccacctc tcaaataagc atactctgga ttataatgag
    69541 ttcaaagata cattcatgca agccgaagaa agaatgaatg ttaaagaagc attaccattc
    69601 cccggagtaa aagaaataat agacagcacc gaaggataca cttttatggt tacaaatcgt
    69661 gccgagtcgg tatttcggtt tttagaatat cgggattaca ggaaatactt taaggagatc
    69721 ctgcacagaa attcgggatt tcccttaaaa cccggcagcg aagcagtcga gtatctgata
    69781 gaaaaatacg gtcttgacag aaaagaaatg ctgtttatag gagacaggga tgtggatatc
    69841 gaatgcgcaa taaatgcaca aatccattcc tgttattacg attcgcataa cattccgaat
    69901 atcgcaccgt gtacatacta cacaaaagat tactttaagt tacttgacat tttgaaaacc
    69961 gcttaaagca ttagtcacaa ttattatagc aacaggaata atatatatcg taaaagcaac
    70021 cagaaacgcc acttttccgt tactgtccag tttcgtctct ttaataatat taatctttgt
    70081 actttcggta ttttttctaa atctcctcag caaaatcact ataaccggta acaccagcaa
    70141 agagatggct cccagaacta taaaagaaac accgtattcg gacagtccgt atgcttcgag
    70201 aaattctata ccgcccgatt ccgggggaat gtttctggtc gcatatgtta tggtaagcgc
    70261 aataaagtta tttataaaat gtcccatcat tgccgcaagc aaggagccgg ttctgtacag
    70321 aaaatagcaa agaagcaccg acaatgcaaa agtggataac agtttttccg gtattgagtg
    70381 agccaaagag aaaaagagcg aacaaaagaa aaagcaaccg accttaccca atctctccaa
    70441 tcccgacata aatgcagtcc gaaaagctat ctcctcacat attgcaggga ccagtgcaat
    70501 aaccaatata ctcttcagat aatcaccgaa gttttccatt aatacaatgt cctcaagtcc
    70561 cggggtggtt ccgaacagat tgtctacaat ccatgtgtta aaagttgcca gcaacatgga
    70621 aacgggtata aacagtgtca caacagccag gattataaga atattctttc cgcttacttt
    70681 tttaaagtgt acattgtctt tgaatctgcc ggtaaaaagc atgtacaaaa gtaccggtac
    70741 cgccagtccc agcacctcac aagccgtctg atacagatag agattgtcac ctaaaaagaa
    70801 aatccccacc gtctgaacgg taaagaatat aatcataacc gcagttatga ggagaaacga
    70861 ttgatagggt tgcagtttgc gacttcttaa gttttccatt attctccttt cagccagtca
    70921 atctgtgcct gtgacagcag gatatcttta acacctatgc tgtcttccaa ctgcgacaga
    70981 ttcttggatc ctattacggc acaggtctgc accggattat ttgtaagata tgccaaggct
    71041 atgcatgtcg gtgatacact gtattcatcg gcaagcattt tggctctctt tgctctttct
    71101 atgttaatat ctgaaacaaa tttcttaact ataccgtctt ttaactgaac ggtattgtat
    71161 tttgagaaaa agccttttgc ctgggaagta tatgccatca acggcaactt tttcttctca
    71221 taccaatcat attgctcatc atcgatacat acaatagttt catcatcaaa tcgctccggt
    71281 gtcgattcgg ctaaggaaaa ataacattgc gataccgaaa gcggcaccaa attctcactc
    71341 gcaatgaaag cctgagcctc ttcaattctt ttactcgtcc agttgcttac acctatgaaa
    71401 cgggttttac cctgctttat aaaatcctga agtatgggta ttatatattc cacaggtttg
    71461 gacaaatcgt ctctgtgcag taagaatata tccacataat caatattcat gacggaaagg
    71521 cttttatcta tatcatattg aagctcttct ttagttattc tttccataac atcttttttc
    71581 aatttcagat tctttggaaa tccgcctttg gttataacta tcaggtcctc cctgtttttc
    71641 cgggatttca tccactcacc cagcatatac tcggaaatat ctttcccttc gacaagccat
    71701 tcgttatagc atcgggcggt atcatatgtt ttaaaatgct cataaaattt atccatcaga
    71761 agaaaatagc tttccttgtc ggcatagtcg gaaaaaacac cggtaccgaa agccagttta
    71821 tccatgctca atgaatgaat cccgttatct aaatcaatct taatcattaa aaatactccc
    71881 ttgcaagagc cgttattttc ggcaggcttg cctgtattgt ctttttggaa atacagaaac
    71941 acatcctcac atagcctttc ataccgaatc ctcttcccgg aacaaccagt atgttatatt
    72001 ctttcgcctt ttccacaaat tccatctcat cttccacagg cgacttaacg aaaagataga
    72061 aagctccttg cggttttttt acactgaatc cgcatttcac catgtgatta tataaaatat
    72121 cccgtctttc cctgtaatcg tcaatatcca ccttggcatc atatgcatac tgtattactt
    72181 tttgcattat ggcgggagca tttacatacc ccaatgttct ggtattgaat atacagatat
    72241 taaccaacag ttgataatcg ggtattttgg ggtctattgc aatatagcct attctttcgc
    72301 cgggcagtgc caatgattta ctgtatgagt ttactattat agccttatca aaacagtcaa
    72361 atacacaggg aaccgttatg tcatcgtata caattttcac ataaggctca tcgcttataa
    72421 caaaaatgtc ggagttatac tttctttccg cctgccttat tgcatcattc agtgctaaaa
    72481 gcacttcctt aggatatacc gctcccgaag gattgttggg tgtatttatg ataacggctt
    72541 ttgtctttgc ggtaattaca cttttaaagg aatcaatatc cggtaaaaag aattcatcac
    72601 aatcggcttc taccaacacc ccctgcgcat gcatgacata aaacccgtat tcggcaaaat
    72661 acggcttaaa aacaacaacc tcatcaccgg gatttaaaac ggatttcagt gttacaaaca
    72721 atcccgccgc tgcacctacc gacattatta tattactgaa aggaacatca taaccgtagt
    72781 ctttttttat actttccgat accgcttttc ttgtggcatc ataccctgca ttgctcatat
    72841 atttatgcat tcctttttct tcactgcagg ctattttctt aagacactct ttaacctttg
    72901 caggcggttc catctccgga ttgcctatgg aaaaatcaaa aacgttttct tccccgtata
    72961 tcgcttttaa tctgtttccc tcttcaaaca ttgcacggat gaatgacgat ttactcaaac
    73021 tctgtgttat cttttcatta aacataacat ctctctcctc ctgccatatt acaatatagg
    73081 taagattttt gcaaattgtg tcatcaacat ataccgattc tattccctca gcttctttgc
    73141 aaccaattca tccgccttta catatgcgga gtagtaattg taataattga catatcccgg
    73201 ctttgcgcct ttgggctttt taacattctt gatgaaagta taatctaccg caacctgacc
    73261 cgatgttctt gcttcggaat gatatgccgc aattatagct gcttcggtta atgtcttatc
    73321 cggcagaaac tcttcgcatt ttgcagggtt ttttattatc acatgagatc cgtgcaggtt
    73381 ctttacatgc aaccatatgt caaagttttg agcgtaatca aatgtaagct tatcgttttc
    73441 ggtattattc cttccgacat atatttcata tccctctttt gacctgtact taacgggatt
    73501 aaaggaaacc gcttccggct gagatgcctt cctgcccttc ttaatcttaa gtttttcctt
    73561 catatagcct tccagaacca attccttctt aatatcctct atgtcggaaa ggttttcaca
    73621 taactccaaa gaagcccgaa ccgatttaag atactcaatc tcttttttaa tacccttaac
    73681 tgcattaacg gcatagttat atgcggcaat ccccttttta tattttttat aataactttg
    73741 cgcatttttg agagcatctt ccgatttgtc cagcggtatc tttacaatcc tgtcggtgta
    73801 atagtctaaa agctccacat aatcggtctt ttgtttaatc gcatgaatat tggctgttaa
    73861 aagttctccg tacagtttgt atttatctat cttttctcta tccgaaagat tgttgttata
    73921 tattaaaagt tttttctccg cttttaccag atgggcggat accgattttc tgaccacatc
    73981 ggatgcttgt ataaaaacag ctcttttatt ttttaagttt aaatggtcgc ttaatgcatc
    74041 aatatataaa ggatatttct taaccgtaaa ctccgatata tttttataca acgaaagatt
    74101 gaagcagtag caatcggtta tggtcttttt ttcattgctg taaacggcaa tcggagaata
    74161 tgtgtttgcc gatatgtctt gtgctgtttt tttaataacg gacataacct gtatcgcttc
    74221 ttccttgctg atattcttaa cagtctttgt ttcatctact ccggctctta cacatatttc
    74281 ttttgcaaat acctgtgcca tacctaaaac cgttcggaat attgcatcac ttaagggaaa
    74341 atccgcatat ttaagtaaat gctcccactg cacatcttct atcggtatct tatcctgtat
    74401 gggaggaagc acatactcgc atgcaggcat tactcttctt accgaagaca tggattcgtc
    74461 cacatgtctt aaagcgtcca atattttgca tgaagagttg acgactatga ggttacagtt
    74521 gcgaccggtc agctccatta tcacggtctt aaccgaattg ccgctgaatt catcggtgca
    74581 atttatatca aagtatatta ttctctcata atcttttgca tatacctcgg atataaatcc
    74641 tcctcctatg tgctttcgca agaacatgca gaaaccgtac ggggttttac gattgtttat
    74701 cttttcttct ttgaaatata tccccggtga agaaggctgc aaagacaatg ccacattgta
    74761 ggatgtgccg ttagcgtata tgcccagtat aacctcatct ttttccggca tgtaaatatt
    74821 ctgaatcttc ccgtttttac atataccgtt caattcattt gcaagacact ttactgcaat
    74881 tccgtccagc ggcatgatat cctccctctt tcaaaacatt gccgttttat attatatata
    74941 ttatcataat atctgtactt taatacttgg tacaggaaag aggatacttt ggaattatta
    75001 tctcctgccg gaaatatgca atgtcttaaa gccgcagtgc aaaacggagc cgatgccgtt
    75061 tatctcggga taaagacttt cagtgcaaga gcaaatgccg acaattttac atttgaagaa
    75121 ttgacggaag ctgtaagata ctgtcatctg cgtgatgtat ccgtttatct gacacttaac
    75181 acacttatag atgacgagaa tatgcaaacc gcccttaata atgcaaagaa agcatattca
    75241 tcaggtatag acgggattat tattcaggat ttagggcttg ctgcacagat aagaaaaatg
    75301 ctccccgacc tgagactgca cgcatccaca cagatgagcg ttatccatcc gaaagaagca
    75361 ttgttactca agagtttggg atttaaaagg ataatagcgg caagagaagc caccttagag
    75421 cagataaagg cgcttgttgc cacgggtatt gaagtggagg ttttcggtca cggctcctta
    75481 tgtgtatgtt actcggggca gtgcagttta agttacttta acggaggcaa atcgggaaat
    75541 aaaggcggtt gtgctcagcc ctgcagactt ccctactcac tgtttataga taatcgtaat
    75601 atctcctgta aaaactatct cagcaccaaa gacatagcgg taatggatga tatagacagg
    75661 cttgtatcgg cgggtgttgt atctttgaaa attgaaggca gaatgaaaag tccggaatat
    75721 gtggcgatta taacaaagac atacagaaag gcactttcgg gtactcttac agataaagac
    75781 agacaggatg ccatgatggc attcaacaga gaaggctttt ccaaaggata tatgttttcc
    75841 gaatccggca aagatatgat ggcatatgct ttttccggaa ataccggtat aaggataggg
    75901 accgtcaaga aaataaacaa agagaaaaac ctgatacaga ttgaaagcaa caaggaactt
    75961 ataaacggtg acgggataag ttttcccgac agctctatag ggacatatgt caatatttta
    76021 agaaaaaacg gcaatctgta cggtttaata tgcagaaata aactccccga accaaatgag
    76081 cccgtatacc tgacatatga taagcaactg accgaacagg cacaaaaatc ctatgccgac
    76141 gacttcttgc gcaaagttcc tgtaagagga catttcatat ctaaagcggg gcaaagagca
    76201 agctttaccg taaccgataa cagcggcaac acttatacct cctgttccga tatagactca
    76261 caagcggcaa aaaaccctaa tgccgatgat gacatattga aaaatctcaa caaaacggga
    76321 aatacggtat ttgaattctc ctctctcacg aaagataccg ataccgctac attccttccc
    76381 gctaaaacgg taaatgcttt aagaagagat gcgctggacg gattagcaga attaaggcag
    76441 agcataaaaa acagaaatag taaaaccgaa ctgtttccca agtatgaaaa cctatcgtta
    76501 tcttcggaaa gttttaatac agagaagaca gaaaaaaatg aaaaaagaga tactccggta
    76561 aacagagata taagtaataa aaaaacaggt atatccctat tcttttttga ggaccgattg
    76621 gattttgata tagcggatat tgatgccgac aggatatatg taccttctac catgtataaa
    76681 agatatcttc gcgatgacag agtgtacttg tatataccca taggagccaa tcctcaagat
    76741 acggcaggca atcggcaaag aattatttcc tcttccatgg ggattttaga tattcctgcg
    76801 gataaagtat tagatgcggg aataaactgc ttgaatactt atactgttga tgctctttcg
    76861 ggatatcaag gtgtaaaagc tatacacctt tcatatgaat tacctgttga agatataaga
    76921 agattcgcaa actttaacgg gaatataagt ctggagtacc ttgtatacgg cagagttgcc
    76981 gtaatgaaaa ccaaataccg tataaaggat atattcaccg ataccgatac acaagaaata
    77041 tatcttttag accgacataa aaaacgcatt gacatagtta catatcccga ctgtgatacc
    77101 acatatcttt taggtgcatt tacggtaaat aatcttttgc ataaagattt aataagagca
    77161 tccggtgcgg atactctcag aattaatgtt tataaagaaa cagcggatga aattaaagat
    77221 ataatcaaaa gagcagaata atcagtattc tataagtttt ctaatagttt ctttaatatc
    77281 tttcgattgg aatattgccg atcccgcaac aagaacattg gctcccgcag cctttatctc
    77341 acggcagtta tccaccgtta cgccgccgtc aacctgaatg tcggttttca gccccatttt
    77401 gtctatcatt ttcttaagag cttttatttt ctccgtgctg tacggtatat acttttgtcc
    77461 tccgaaaccg ggatttacac tcattataag caccatgtcc aacataggca gaacatattc
    77521 caaaaccctt atatcggtgg cgggattaag agcaaccgcg gctttcttgc cctgctcttt
    77581 tatgtacctt acggttctgt ccagatgaat gcaggcctcc tgctgaacgg ttattatatc
    77641 cgcccccgct tttgcaaatt cttcaatgta tctgtcgggg tcggaaatca taagatgaac
    77701 atccagcgac aattttgtta tgctcttcaa agcctgcaca atgggaatgc ctactgaaat
    77761 attgggaacg aaatgtccgt ccattacatc cacatgcaca taatgggctc cgccctcttc
    77821 tatttttctt acttcttctt ccaatcttgc aaaattacaa gccaataatg acggtgagat
    77881 ttttatcatc aatatcccct ccttactctt aaagcttctt cagacatatt atatatcatt
    77941 tcatacctta agtggcgttc aatcggaatt tcgccttttt ccaccgcctg cagtacggca
    78001 cagtggtcat catttatgtg tttgcagtca ttgtatctgc actgcccgat gtacggcagg
    78061 aactcattat acataccctt tatcaggtcc ggctgcagaa gaccggggtc aaatttggaa
    78121 aaacccggag tatctacgat atagccgcct tcaaaggaaa gcagctcaca atgtcttgtg
    78181 gtattctttc cccttcctat cttttcactc aaaatcccga tttccatatc ggccgttcct
    78241 gttatggcat tgataagtgt ggacttccct gctcccgact gacctgcaaa aacactgact
    78301 ttcccgctca gcatactctt taacggatcc aaacctgtct tatgttttgc atcggttatt
    78361 actattttgc atccgctccc ggaatattgc cttaatacat cgtcaatatc ctcttttgcc
    78421 gacaaatcgc atttgtttaa acataatatc ggctctatat cgtttgtacg aaatgccagt
    78481 atcagcaaat caatcagcag ttcatccaat gtcggatatt ccgttgcaaa cacaataacc
    78541 gcaacatcca cattggcaac gggtggtcgt accagactgt tctttctttc ttctatactt
    78601 atgatatagc cttccttatc ggcataatcg gttatatcaa aacgcaccaa gtctccgcaa
    78661 agaggcttga atccctcttt tctgaacaac cctctcggtt tgcagatata ttcatcttta
    78721 tcggttctta cggtgtaacg gttgctcagc gcttttgtga taagacctgt ttccatttac
    78781 ggttccgggg tttctttata gtggtcgaaa tatttgtata ttatcggttc tttatagata
    78841 accgagccta taaatacagt cattttggtt tcgccttttg caggtatgcg gacatctctt
    78901 tgatagggca acttttcaat ggaagaaata accgcagttg tctgaaaatc ctcatcctga
    78961 tcggaatact cgaatttgaa cctcaaggtt atgggcattt ccataccggc aagttccgct
    79021 attgtagggt caaatgtgta tttaacggtt atatcttcat acacggtatc tccgtaatcc
    79081 accgagagct ttatatctat ggtactgttt tcatatacct cggtatcggc tgccggatac
    79141 tgataagata caagagactc tttaccgggt acctcttctt ccgacattac attcattatg
    79201 aggtttcttt ccagtaacgc tcccgcagct ttctcctttg tcaacccgaa cagatccggc
    79261 accgttgtct tttcaaattc ggaaccgaga gaaacatata ttttaaccgt ttctccgtat
    79321 ttaactttaa tcgtttcaga gggagatacg gcatacactt ctcctgccgg gatatcctgg
    79381 ttatattgtt ctactttttc cgggataagc cctgcattta ttattatatt ttccgcaata
    79441 cgataatcct taccgacaca ctcgggaact tccacatagt tacttcccac acttaccgta
    79501 aatgtaatgg tattcttggc accttctttg aaaattctgc ccggttccac atcctggtcc
    79561 attattatac cgggaggtgt ttcatcggag tatgcttctt tcattaatac agaaatattg
    79621 aaatccgttt ccagtttctt ttttatggtt tcatactcgt atgtaacata atttaatact
    79681 tcatactctt taggttcggg aaccactact ttagccatct catctattgc gaaaacaata
    79741 aaaaaggccg ataatgacag tacacagatt aaagctataa gagtggtgaa aaagtatttc
    79801 ttctctttct tttttctgcc tcttgcgtaa atcaggggaa cattgtactc ctcatcgtaa
    79861 tttatcagtc tgggaccggt tattttatcg gttttttctt taattttgat tacttttccg
    79921 gtatttcctc tgacaattgc attgtcttct tctaaaaaca aatcatcttc cgtaacttca
    79981 ggcaaggtca ccgtttcatc ggaaggcaga acctctccgt ttataaagtc caatgtaata
    80041 tcgcctttag aattggcatt attattcagt ttctttaaat ctgccattac atcggctgct
    80101 gtctgatatc tgtccgacgg ttttttctgt attgccttaa gaattatctt gttgagttct
    80161 tccggcatat ctcttctgta atcaatcgga ggtatgggag tatcctgcag atgcttcatt
    80221 actatggata taggagtgtc ggcatcatag ggcacatgcc ctgttgccat ctcatacata
    80281 actattcctg tagaataaat atcggtgctg ctacttaaaa aaccgccttt agcctgttcg
    80341 ggagatatgt aatggaccga tccgaatgtt ttattatccg tggtcagagt ctgcgcattt
    80401 atcgctttgg ctataccgaa atcggttact tttattcttc cgtttttgga tatcagtata
    80461 ttctgcggtt tgatatccct gtgaataata ccgttgttat gtgcatgttc caaagccgca
    80521 aaaatctgca tggaaatatc caccacttcc tttatatcca aaggtcctgc cgactccaga
    80581 tagttggata gtgtggtacc ttcaacacat tccatgacaa tgtagttcat gttattgtca
    80641 tatcctacat catagacatt gactatgctt tcatgtgaca gttttgctac cgataatgct
    80701 tcatccttga agcgttttat aaaatcttta tcctgagcat attcatcttt aagcgttttt
    80761 atcgctacaa ttcggttgag cgctttatct tttgccttat atacataagc cattccgcct
    80821 atgcccatct tgcttagaag ctcatatctg ttattgagta taacaggatt cattttattc
    80881 ctccggatta ttgtccgctt tgataagaat tatggtgata ttgtcggttc cgcctttttc
    80941 attggctttt tgcgcaagat ccttgacttt ttcttccaaa tcggtttcct gattaattat
    81001 attcaaaata tccgtatcgt caagcatgtt ggtaagaccg tccgtgcaaa gcagtatata
    81061 ttcacagcaa tccaatgtca tatcatagat atccggttcc ggcacatcgg atatgcccag
    81121 tgctttggta ataatatttc tgttgggatg gttatgggat tcatcaacgg ttatatcgcc
    81181 tttatcaata agttgctgaa catacgaatg gtcttttgtt atcatcttta tttcattgtt
    81241 atacacggta tatatccttg gatcgcctat atgtataaca tgaagtttat catcttttat
    81301 gtaaatggca tccaaggtgg tacccattcc ggaaaagaca ttgtgctttt tactgaagtc
    81361 cagtatctct ttgttagccg ttttacatat attaaagaga taatcctcta tattgttgca
    81421 gttcttgaag cgtctgtccc ttattttctt ctcaaaacag tctattgctg ttttactggc
    81481 tatctcaccg aaaagatgac cgcccatacc gtcggcaacg gcagcagcaa attcgttatg
    81541 ctcatcaaag ggaatgacga tatgataatc ttcattattt cctcttacct gcccgatatc
    81601 ggtatacgaa gcgtaattaa atatcatgct gtctccttaa ctgcccgcat gcggcttcaa
    81661 tgtctctgcc cagttctctt ctgatggtga cgggcacgcc atgtgctttc agtatagcac
    81721 aaaaaggcga aagatttttg ctgttttgaa ctgttttcat attttcaaca ggatttaccg
    81781 gaattacatt tacataacac attacatttt tcaataaagc actcaagcgt cgcgcatgct
    81841 ctaaggagtc attagacccc gggatcagag catattcaaa agaaactctc ctgttggtct
    81901 tctcgaaata gtattcacat gcttttataa gggaagagat gttgtatttt ttatttatcg
    81961 gcattattgc cgagcgcctg tcatcggtaa cttcatgcag cgataccgac agattgacct
    82021 gaagacacaa atcggcgaac tcatatatct tgggtactat tccgcaggtg gaaacggtta
    82081 tgcgtcttcc tcccatgtta aacccttctt tcatgttaat aacattgaga aaatttacaa
    82141 gattatcata gttatcaaac ggctccccta tacccattac caccgctctg gatatggtct
    82201 tgttgctgtc ctgtgccgct ttaagaatct gagcggtcat ctcataaccc cacaaatccc
    82261 ggctgaaagg tactgttgat gaggcacaga atacacagcc catcttgcat cccgattgac
    82321 tggaaatgca gatggaggtt ccgtaatcat agtccatcag cacactttcc accgttgcat
    82381 tatcggaata acgaaaaaga tatttaacgg taccgtctac cgacacaagc ttcttttcta
    82441 ttacaggtaa ctccaaagaa aagtttgcct gcaaaatctc aatatcttcc ttacttatat
    82501 tggtcattaa ggaataatca aataccttct tgttatatat ccagtctaaa acctgcaaag
    82561 ccctgtacct tttcaaaccg tattcctgaa aaaggtcttt aagttcgttg tgtgtatagg
    82621 aaagtatatg aatagaagga gcgctcatgt cgaaaattat atatttttat caaaagaaaa
    82681 tcaatgcact catcattatt cgtcacaacc caaaaccata tcacacggca tattatgacc
    82741 gcattttgat atatgcattg cctttttatt ctcaaactgt aactctgtta tatacaatat
    82801 gccctttttg catttaacag ccattctctc tttatctatg gatataacag taccgttgtc
    82861 aacatcttta ttatagtctt ccaaagaata atccgccttt aacactttca gtgtttttcc
    82921 gcagtaagtt gtaaatgttc ccggccacgg attaaatgct cttacaagat taaacagtct
    82981 gtatgagtca aggttaaagt ccaatctgcc ctcttctttt tttatcatgg gagcatatgt
    83041 ggctttatcg ttatcctgct taacaggcgt tatcttatcc tgctcataca gttttacggt
    83101 gtctataagg agttttgctc ccatcaaaga aagtctgtca tgtaaagaac atatatcatc
    83161 ttcctctgtt atgtcggtct ccattttcaa cagcatatct ccggtatcca ttcctatatc
    83221 ggtcagcatt gttgtcacac cggttacctt ctccccttct attatcgctc tttgcatggg
    83281 ggaggcacct ctgtatttgg gaagcagcga tgcatgaaca ttaatggtgc cgtattttgg
    83341 tatatctaag atactttgcg gaagaatctg tccgaatgca caggtcaccg ctaaatcgca
    83401 acctatcttt gaaatggttt ccacccattc tttcttgcgt attttttccg gttgcagaac
    83461 aggtatgtca tactctatcg ccttcttctt tatattcgaa tactccacaa cattatgcct
    83521 gcctttgggt ctgtcaggct gtgttataac ggcattaacg gtatatccgt tttcaatcaa
    83581 tgcaataaga caaggcaatg caaaatccgg agtacccata aaaatgatat tatttatctt
    83641 cttcactctg cttttctctt tcttccaaat ccgcaggatc aatttcctca ataactttat
    83701 ctctgaataa aataccgtcc atatggtcca attcatgaca gaagcaatga gctgcatatc
    83761 cctcggcttc ggcatattgc tcttttccgt acctgtcaag atagcgtacc tttatcttca
    83821 taggtctttt gaccgttccg taataatccg gcagacttaa gcatccttcc acatcgatat
    83881 cttcgccttc agattggatt atgacggggt ttatcaactc atacttctta tcatctattt
    83941 caataacggc aacccttctg agtactccca cctgtaccgc agccagtccc aatcccggtg
    84001 cagctaccat tgtttcatac atatcatcaa ttaaagtatg caatctgtca tcaaaagatg
    84061 tgacttccct ggactttttt cttaatattt cgtcgccttc cttacgtatt gtccttaatg
    84121 ccattttctt ctccgtattt aacacaataa ttttatacta tcatcgacta ttaaacaaca
    84181 tgagctgctt ttcacaatct gcttaaagtg gaagttattc tatttattga gctacgcaag
    84241 gaagatattt ttttatccaa tctatctatt tcactaaata tttcaataat atcctgatct
    84301 tcacaaatta aatagccatc ggaaaatgcc cctggtaatt caaatttctt ctcacctttt
    84361 gcaaatttaa ctattattct atcctcatct tgcgatatga ctgttcctgg cccaaaaacc
    84421 ttatgccgga ttttccaacc cacaacatca atatcatcaa tcttacttct ttcaataagt
    84481 gcagtatcta actcggcatt aaccgaatca agttcaaggt ttaactcttc cgcttttgca
    84541 tttcgttgtt gctttaccga tgacttttta acaggttttt tgatagcaat gtctgcgtac
    84601 agtttgtata ctgtgacaca ataaatgata tcaaaaacca taatgtgata atcatcgtca
    84661 gacaacattg tatccgtcag tcggttcttg tgagcttgca taagttcagg tgtatctttt
    84721 atcttctcaa gaagtgcatc gcacatacga taataacggg ataaattaaa actttttcca
    84781 cttccgaaat catcaggata ttcgatacag tacataaact ccttagcttc tgttgcctta
    84841 aagaggtaat tctctttagg ataacgcata ttaaggtagg acaatactgt tcgaaaatca
    84901 tgctggtacc tccatttgcc gggctcgtat ttatcaagta agtcatttac cctatctctg
    84961 aaatcttcaa tccgtttctg acgttgtaca atattgccgt catcagcaat aaacagcatt
    85021 ctaaacattt cccttataac ttccgtaagc tcagatcgct cggcaagctt cacaataccg
    85081 ttggtcgggc tcactgcctt attatctatc aaattgtatg tcagtttcac cgactcctta
    85141 aacatttcta caaaattctc tgcatcaata tcccaattat ctttaaagtg tttaaatgct
    85201 tcccatttat agtattcttg atgctcatca ccatttacat agtcaaagtt gtcaatatag
    85261 gcctgtacaa ccttatccaa attagtagta ttcatgtcat atctcctttt caaaatttca
    85321 caatctccaa atatctgtcc agttgctccc ctacatattc cgcaataagt aaaaccatat
    85381 catcggcttt tctgtcacga taaaatgcat cgaaagcatc ataataccgc ttgcgatccg
    85441 taaacttaac attgatagcg ggataaccgt tgcggataag ttcaagattt aagattaacc
    85501 gaccggttct gccgttaccg tcaataaacg ggtgtatacc ctcaaattca agatgaaaac
    85561 gggcaatgcg ttcaatcggg tgcatagtct tttttctttc ctcatttctt gtaagtaagt
    85621 ccgtcatttt cggttctata agatatggct gcacagggac ggcatatgca cctgtaattg
    85681 taacgggaat acggcggtag acacctttat catccggtct gtccatcagc actaaagaat
    85741 gaatggctct tatttccgta tccttaagac gcactttgtt ttgcgcaata tcttcgatat
    85801 attgaaatgc atcacgatga ccgaccgctt caagatggtc ttttaacggt tttttatcta
    85861 ttgtcatacc ctcaagtacc atagcggttt ctttgagtgt cagggtgtta ccctcaatgg
    85921 cgtttgaatt atacgtgaag ttcaccataa agtcgttgcg taaacgcttt atttctcccg
    85981 cggtaagagg gcgcatacta tcgagtttta cttttttaat atctattgat gtcagtaaac
    86041 ttgcaaattt gccatgcttt cttttgtgag gtaatttccg cttatccaac ggcttttcag
    86101 tatcgcaagg tatcatatat aagttgcctt tacggataac tccttcgatt tctccgttgg
    86161 cacaaagcag gcgaacacga cgagtggaaa cattccattt ttcagctgct tgtgtaaccg
    86221 ttaaatattc cacctctcat acctcctgat tccggaatat taaatataaa caaagtataa
    86281 tgcataatcg gaacaattac aatacagttt ttaaatattc ttgtattagt ttgccgattt
    86341 acagttatct tttgctttta ttctaatgag aaactcccac ctgattggcg ggagttttct
    86401 gcgaaattat ggttatatta tttattctgc aaatatagtc ttttccgact tcttgtcaaa
    86461 tatgtgtact ctgaccgtat ctatagctac cttaatggta gatcccggtc ttgcctttgt
    86521 ggaaggactt actcttgcaa taatattgct gtctgcaaat gagaagtaca acagagcttc
    86581 cggtcccatc atttccacaa catcaatctt aacctctatt gtggtttcag gcatctggtc
    86641 aaggaatacc tgctcatcac ggaaatgctc cggtctgatt ccgagtataa cttccactcc
    86701 gtcgtatcct ttaccgatta tactctttgc tctcttttca gagaatttaa gcttggttcc
    86761 gcctatgtct atccatacat ccttgccttt ttcttcaatg gttgcattta agaagttcat
    86821 ctgaggtgtt ccgataaatc ctgcaacata catgttaacg gggttatcat acataatctg
    86881 aggaggatca acctgctgaa taaatcctgc tctcataaca acgattctgg tacccattgt
    86941 catagcttct acctggtcat gtgtaacata tatgaatgtt gtcttaagtc tctgatgaag
    87001 ttttgtcaac tcaacacgca tctgtgctct caatttagca tcaaggttgg aaagcggctc
    87061 gtccataagg aagactttag gttcacgaac aatagcacga cctaatgcaa ccctctgtct
    87121 ctgtccgccg gagagagctt tgggctttct gtcaagcaga tgctggattt ccaatatctt
    87181 tgctactgca tgaactctgt gacgaatttc atttttaggc atctttctta atttaagacc
    87241 gaatgccatg ttatcgaaaa tggtcatgtg aggatacaat gcatagttct ggaaaaccat
    87301 tgctatatct ctgtctttgg ggtgtacttc gttgattatt ctgtccccga tataaatttc
    87361 accggcggtg atatcttcca atcccgctat cattctgagt gttgttgttt ttccgcatcc
    87421 cgacggtccg agcagaacta aaaactccct gtcttcaata acaagattga agtcggaaac
    87481 agctgttatg ccgccacggt tgggctcata ctttttatat acgtttttta atataacttt
    87541 tgccatatat gattcctcct attcatttct tcgtatattt tatcacttta cgggtaggaa
    87601 tataagatgg tatgtctaca aataaatatc gctctttttg tacatcttgc ataaaaatcg
    87661 ctatttgtga agtatcggcg acagcctttt atagataagg aaagttataa caccgctcaa
    87721 tacccctttt aagagattaa acggcacatt tgccaataag ataagatccc aaagagagtt
    87781 tatgttagga ttgatctttg ttcccatctc aactattgcc tcaaccggca ttccgaaagc
    87841 ttttgcatat gtaggtataa gaagaaaata attcaacaac gctccggcca cagtcatgga
    87901 tacgatacct accagcatac ctataatggc attctttata ttcttcttcc ttttatatat
    87961 aatacctgcc ggaaccacca gacagcaccc tattaagaaa ttggcaaatt cacccactcc
    88021 ggcagtggct gtaccggtta tgacaaggtt gaccaatatc ttaaccgcct ctatgattat
    88081 tcccgccacc ggtcccattg cgaaagttcc caaaaaaacc gcgacctcac tcaaatctat
    88141 ttcatagaaa gcaggagcaa aaggcaacgg gatttccaga agcataagca ctcccgctac
    88201 cgctcctaaa atacccattt tagccatgtt tcttgttgtc ataagttttg ttttgtgctg
    88261 ctccatatcc gtctcccaat cgatactcaa gcaaaagtat aacacaagga gtggaataaa
    88321 tgtataatat agttataaaa caaaggagga ggagtataag cagtccgata ttgcaggtaa
    88381 cgactgagtt atacaaattg atagcatgaa attaggtgtt tttaccgttg ttttaagctc
    88441 tatgagtttg gaaaatgcaa taatttactt aaaagatctt ggtgttcagg cgattgaatt
    88501 aggcacggga ggatttccgg gaacggcgca tcttgatgct aaaaagattt ataacaagcc
    88561 ggaagaaata aagaagatta aaaaattgac ggaaaaatat gacatggaga taagtgcttt
    88621 aagctgtcac ggtaatgcag ttcatcccga taagaaaata gctgccgcct atcaggagga
    88681 ttttgaaaaa acaataatcc tggcggagaa actgggagtg aatacggtag taaccttctc
    88741 cggttgtcca ggtgaccatg ataatgcaaa atatcccaac tgggtgacct gtccctggcc
    88801 cgatgacttt ttaaagattc tggattatca gtggaatact gtacttatac cgtactggac
    88861 caagcaggtg gaatttgcaa agaaacataa tgtgcatcag attgctttgg aaatgcatcc
    88921 gggtttctgc gtatacaatc ccgaaacctg cctgaaactt agagatgccg taggaccggt
    88981 aataggagca aacttcgatc cttcacacct tttctggcag ggcatcgata tcatcaaagc
    89041 aatccgggca ttaaaaggag caatttatca tttccatgcg aaagatacaa aaatatatta
    89101 ccccaattcc caagtaaacg gagtcttgga tacaaaacct tacagcgatg aaccgcagcg
    89161 gtcctggctg ttccgcactg tgggtaccgg ccatgacaga caggtatgga atgatatttt
    89221 tgcggaatta agaatggctg aatatgatca tgtaatatcc attgaacatg aagacagttt
    89281 aatgagcggg caggaaggcc tgaaaaaagc tatctccttc ttaaaagaga caatgatttt
    89341 tgagcccaaa ggcactatgt attgggcata aactcctgct aagcgacaat cgaaatatat
    89401 acaagtatat taagatatct ttgaacccgt aggcatttcc ctatctatag tgactaaaga
    89461 aagtctgtct ttatccgatg cggctaacag cataccttcg cttgtctcac cccgcaacac
    89521 ggccggcttc aagttggtta taaccacaac ggtttttcct attaactcgt cacatgtata
    89581 atactcggct atgcccgata ctatctgacg ggttttattg tctgtttcca cctgcagttt
    89641 caaaagcttc ttggctcctt ttacaggttc acaacttact attttagcgg tcttaagttc
    89701 aatttttgca aaatcctcaa tccctattaa tgctacattc aggtcttgct ccttttgttt
    89761 gtcactcttt tcaatttcct gcagtttctt ttcggtatct atcctgttga agagtatctc
    89821 gggtttccca agtttaatgc cgtcttccaa ctttccgaaa gtataggttg aagcaaaagt
    89881 tgtttccttt gtattgagct gtgaaattat cttatccgaa gtggaaggca taaaaggata
    89941 cagtaaaacc gataccaccc ttattccttc taaaagattg tacataacct ttttcagtct
    90001 ttcgtggtct tcctcgtttt taaaaagtgt ccagggcaga gtttcatcaa tatatttatt
    90061 gcaacgcttt gccaaagtca taatctcatg aatggcttca ctgaccttat accgcatcat
    90121 caaatcacaa actttatcag gcgttttctc tgtcagctcg ataagttctc tgtccgcttg
    90181 gttttccggt agcagcgatg cgggcttttt aacagtgcct tcaaagtatt tgtgttgcat
    90241 tgtaacggtt ctgttaacaa gatttcccag aacattggct aaatcggaat taatcttctc
    90301 aattatctgt ctgtgcgata ttatgccgtc attggcataa ttcatctcac ttaatacata
    90361 atatctgact tcatctacac cgaaaagagc ggaaaggtca tcggcatata agatattacc
    90421 tttggatttg ctcatcttgc ttccgtcacc gaaaagcagc cagggatgtc ccaaaaccgt
    90481 tttcggaagc ggtacatcca gagccataag catggcgggc cagtatattg tgtggaatcg
    90541 gacaatatct ttccctataa catgtacatc acaaggccag tatttattga acagctcgct
    90601 gtggttgccg tcggcatcat atccgatacc ggttatgtag ttggataacg catcaatcca
    90661 gacatagacc acatgctcag gatcaaacgg taccggtatt ccccacttaa aagatgtacg
    90721 tgatacgcat aaatcctgaa gtccgggttt taagaagttg ttgaccatct cgttctttct
    90781 ggactcgggt tgtataaaat ccggattacg ttctatatgc tccatcagtc tgtcctgata
    90841 ttttgacagt ctgaagaaat atgcttcttc tttggcttta ttgacctctc tcttacagtc
    90901 ggggcacttg ccgtcttcca gttgtgattg cgtccagaaa gactcacacg gagtgcagta
    90961 ccagccttca tattggcttt tataaatatc ccctttatca tacagctttt tgaatatttt
    91021 cctgatctgc tgctcatggt cggcatcggt agttctgatg aacttatcat agcttgtatt
    91081 cataagatcc catattctct ttatttcctt tgcccacccg tcaacaaatt gcttgggagt
    91141 aatgttttgt tcctttgctt tttcctcaat cttctgcccg tgttcatcgg ttccggtcat
    91201 aaagaaaaca tcatatccta acatccgcat aaatcttgct atgctgtcgg caagtattat
    91261 ttcataggta tttcctatgt gaggttttcc cgatgcgtat gctattgcgg tggtgatata
    91321 gaattttttc attacttact cctttttctg tcttctttca gtctttccgt taccgaacgg
    91381 gcggcaagcg caccgtcgga tacagccgtg gcaacctgca tataggtatt tatgcagtct
    91441 cccgcagcat atattctgtc tatattggtc atcttggatg aatctacaac aatctttccc
    91501 ttttccgtag caatacctga tttcaaggaa aaggaggaag catccgccgt accgtatgca
    91561 atgaatattc cgtcaaattg ttgctttgaa ccgtctttga actcaacacc ttctacactg
    91621 tcttgaccat atattctccc aatctgctta tcaattaccg gtatttcgcc cggtacatca
    91681 aattccggat tatttccgtt ggttaaaaga tatacgtctt ttgtgaacat taacagattg
    91741 ttaagctcat gttctgcata atcggtatat cctattatcg ctgctttttt attgcggaag
    91801 aaaaagccgt cacatgtcac gcaataactt atacccttcc cttcaaggtc tcttaagccc
    91861 ggcacattga gacttcttct ggtcattccc gctgcaatta tgacataaga agcgacatat
    91921 tcccctttta atccctgtac cgaaacggaa tgctcggaag gcaacaacat gaccacctgg
    91981 tcttctttga actccacatt aaaccgcctg gcctgtccca ctccttcttg gagcagcttt
    92041 tttgcttcaa caggtttacc gaatccgtaa taattctcga cataatcggc tttctccaac
    92101 gaaccgccgt ctttgtaaag tataagggtt ttgaatcccg ctcttcccag atagatgccc
    92161 gcttgtgctc ctgcaggtcc cttgcctatt attatgacat catacatttt tttgcctcct
    92221 tgaatcagct gttaaccctg taaggctcaa taagcttatc aaatgtataa aaggggaaaa
    92281 ccgcaaagaa actgaaatat atagtataaa gaacaaatcc caccagaaat tccaaaacct
    92341 tgttttctat cttatacata acgaagagca ctcccgcaag gaaaagcagc agtaatgcgg
    92401 atttggcgaa ggttctccag tatcgcgcat taacggcagc attggccttc tctatgggat
    92461 atctggaaaa atggaatatg gacggaaact taaatgcgaa agtcatgtat atcaaaatga
    92521 aaatggcaat gataataaat atcagcacag caagtaaaat atacacgacg gtagggtcat
    92581 gtcccgcatc cgcaacggaa ttctttaata tgaaaaacgg cgtaaatgcc aacggaataa
    92641 aggctacaaa cagcaacagt gaaaggtaca tctgaaagaa taccaatgca catctgaaaa
    92701 atcctttttt taatccctga agataatgag taaaatccgt cttaattctt ttaaccgtca
    92761 gatgaattat gttcatatag ccgctgaata caaggctgga cagcaaggca agaactaccg
    92821 ccagagccag aatacccagc agtatatacg ggatatgggt atatttatat gcccatttgg
    92881 aaaacatgat aacggtatcg cctatgtcat cggatataaa cgatgaatag tatttggaaa
    92941 gcagttttgc ggggttaaaa atcattatta tgcaacaaac taatgatata catgccgtat
    93001 aaatgggtat caaaggcttt tttataaaat atactaatga attactgaaa aaattcatgt
    93061 ttcctccatt aaaacattgt gattatatca caggggtata ccctttgcaa taaatcgatc
    93121 agttatagca actgtctgta ttcggtaggc gatataccga aatgtttctt gaacagtttt
    93181 gtaaaatgtg caacatcctt gtatcccaac ttatcggata tttcatacac tttcgcattg
    93241 gttgtcttta aaagtttctt tgcttcgttc atcttaaacc gtattacatg atcggtaaaa
    93301 gattcctcgg tgacctgctt aaatatctta ctgaggtagt taggatgtat ctgaagcctc
    93361 tcggcaatgg taaccaatga aacatcctca taaacacttt cctttatata ccttaatcct
    93421 ttattaacca ggaaattgtt ctgcttgctg tccgattctt caatggcatg aataatctca
    93481 tttattattc ctaacataaa tatcttcatg tcgtcaatgg atatcagttc attaatttca
    93541 ttgtataaat cattttcctt gttatagaag cggtaattgg atatctccag cgagtatatt
    93601 acccttataa taaagaccga aagtaagaaa gctattcgct ctttctggtg aacatcattg
    93661 gcggctatct gcctggaggc ctgcattatc tgctcgatta ttttcttcgc ttcctcctgg
    93721 ttgacattcc gcaatgcaaa aaccatatct tcttcactgt ttttcagaat gttatcaaaa
    93781 tcctccacaa ccattttctc tttggaagca tttattttct gctccgcatc aaccactaca
    93841 tttttaccta agactaccct gtaaagcatg gcatccatag cacttatata tgaaagagca
    93901 actttttcca cgcttccgca ggtgttgcct atgcctatgg ataacgaaag tcccatattt
    93961 ttgtttaccc agcttttaac atcatttagt ttgttgataa ggatgcttct ttgtatctgc
    94021 tgattggagc atatgagtat gcctatgcgg ttatccagat taaatgtata acatttgtaa
    94081 atctcctcaa gattctcctc tatcgtactc attatagccg ctttatagaa agagaagtca
    94141 tatatgttca gttttttctg agatgcggca agctcaaatt ccgctacaca tacaatatag
    94201 ttggtgtaat taatatctat ctccgctatt gcggctctgt ccattatatc ggctctctgt
    94261 cttgttcttc cttccataag taaacggaag aaattatctt taagaaccat tatgttctcc
    94321 cgcaacttgc gtcggatatt atcattaagc cttacttcct gaaggcgtgc gtcaatctcc
    94381 tgctttgctt ccagaacctt ttgaataagc gtctcggcaa ccaacggttt tgtaagatag
    94441 cagaatgcgt tattggtaac ggcccttctg gcatactcga aatctctgta tccgcttatc
    94501 aggataactt taatgttggg atatgttctg ttgactatct ccaacaaatc cagcccgctt
    94561 atttccggca tggaaatgtc ggttattact atgttgggca tgtatttttc tattaaatcc
    94621 agagcttccg atactcccga tgcaaccccg catacattta tgccgtgctg ctgccaggga
    94681 atggatgttg caataccttc ccttatggct ttttcgtcat caacaataat aatatcaatc
    94741 atcttcttta cgctcctcgt acggtacacg taagataact gtggtacctt ctttgtactt
    94801 actcataata gtaattccgt actgcattcc ataatagagt ttgattctct tctgtacatt
    94861 tcttaacccc gattcgctct ccagtttggc ggaatttatg ctggatgtta tcaggttaag
    94921 cttttcctca gtcattccga taccgttatc tattaccttt attattatat caccttttga
    94981 atcatcgtca tttttctcat gctctacata tatcttaaga attcccgagt ctctgtcttc
    95041 caatccgtga ataattgcat tctccacaac agtctgaatt atgaggttaa gtattacaca
    95101 ttctttaagt tcatcggggc agaatatatg gtaggaaaag gattctccgt accttattct
    95161 ttgtatgtca agatagcatt caataccttc caaagcatct tttatggtgg ttaaatcttg
    95221 tcctctgtta aatattttcc tgtacatggt ggaaagggcc acaactattt tagatatttc
    95281 ttcaacatta tgctcttttg cagtccagtt aatggtatcc agagtattgt acagaaaatg
    95341 cggatctatc ttgtcccgta aagtctgcag ttccagctgt tttctgtaaa gttcggcttg
    95401 gtacagctct tcaatcagca cagtagctct gtccgtcatg gagttgtagg catgaaacag
    95461 accttttatc tcattatcct tacatatgct gtcatccagc ttggaggtaa aatctccgct
    95521 gcctactttt tccatacctc tgatcagccg ggaaatgggt ctttgaacat tggtttccaa
    95581 tattcttcgg aaaatagaat agcacaatat aagaataaac aaagaaactc ctatcgagat
    95641 tataacggta tacttatata catcattcgc tatatccttt tccgggataa ggtatacaat
    95701 tcctaaatca taatcacaat ccaatggcga atactgcagg taatacagca catagttatg
    95761 ttctattgtt actttttctt ttattaaaag gtcgtcgaaa taatcaaaag cctgtcctcg
    95821 cgtcttatac ataagtatct cttcgctgcc gctgaagaaa aagttgtcgt ctttggaaat
    95881 aaacccgttt gttgagttct ctgtcaaaac aggtccgtca agaaaccgtg cgaaatccgt
    95941 ttgtgtaata ttcaatgtta taaaaacacg acgattaagt gacggattga gaacatcggc
    96001 cttgtatgtt atggctatat attcatcata aatatgccac atgcagtact tatcaagatc
    96061 agccgaaggg attcttcctt ccacacgttg gataaaccaa gggaatccgg ctttatcaac
    96121 atatgcggca aactcatttc tgccgttcag cagatactca agttcataat cgtaaatcat
    96181 gatatggtct atatattcat ttgtattgct cataagttta aagtagcgga aaatatcgga
    96241 attatcatgc attatatcat cgagataata aatgttatcc gcatattccg ataacatatc
    96301 gctgtttgcg atattgcttg ctaccgaatc aaacatctcc acttgctttg tatacatcaa
    96361 aacgatacgg cggtcggttt cttccaacag cctgttttta tcgcgctcgg caattttagc
    96421 caggattata ttattggccg ttatcaataa taaaactatg gcaaggcata tgaccaatac
    96481 accaagcaat ttgtttttta agcttttttg ggcagtatct tttctcagtt tttatctgtt
    96541 cctttcttat cccaacttac tcctgacaag atcgacagcc ttttttgcat cttcaacatc
    96601 gcttgcatca taccagacga tgactttctt gtcccttaaa aagttcagat tatcaattat
    96661 ttcctctttg gttaaagcta cagttacaat cttattactg agttccttct catacttttt
    96721 ataaacctca atacctctcg gtgcattggg atcattagat atttccacaa actttatctt
    96781 atccacttcg caaaccgcag gtatgacatg gtgtccgacc gaatgtacat ggaagaataa
    96841 tccgtcaaag ttatcaccta ttcgttcaag aaacggaact tcgaattttc tgaacatctc
    96901 atcggacaac atagttgtcg catccaccga aagatgcccc gcataacctt tcggcatgat
    96961 aacatcaaat cccgatataa catatccttt gtactcggta actattttct gctgctcttt
    97021 cagcatgtat attattgcat cggtgcattt tgatgtaagc tccaacaaac cttcttcttc
    97081 atcataaaaa tcgtaaaata tatcgtttcc gcgtaatgca ttgaccacat ccaatggacc
    97141 gtccgcacct ctgaatctta taaagaactc atctttcacc ttgctcttca tatatttcat
    97201 tccttctatt acaagattcc tccatatatt tgactcatca accttcgcaa gagtgtaatc
    97261 tgagtaatct ttcacatgtt gatgatgcca tgaagtggtt tcggaaaagg tgacgtctcc
    97321 gcccataaaa gctgaatgtt ccgctatacc gtagcgtacc gtaatggact gtattatatc
    97381 atcgggtatt tggtttcgag tgtcccagaa gatacgctgt ctttcaatca tataatccag
    97441 ataagtttcc atattcttag gtaatccgaa actgtccaac ggacgaaaag gcacatcaat
    97501 tccgctgaat atctccttaa cctgtataag ggcacctttt cccgactcat acatatcctt
    97561 tgttcttttg attatctcat ccgttctttt gtaatccata caaaacctcc gcttcagtat
    97621 tttactgcta acagtatacc cgttttaaag attgccttca acatcaaaaa ggattagtta
    97681 atccgataga tacctcttca ataaaaaggt gcaaaaatat ggaaaagtat agatgtcctc
    97741 gctatacccg atattaccgc cggtaagttt aatgccgtaa ctgatgtcgg aatatctatc
    97801 ctcgctaatc agcattctca ttgatttggc agttccgcct ttcgccttta cctctatcgg
    97861 gataagagat tttgccgtcc ttacaaagaa atcctgctcc aaagtggaat cgttccgctt
    97921 gtaatagtag agactgtatc cggatttttt caatgcttcc cccactatat tttcatacag
    97981 tgcacctttg tacactccta agtttctgtt agctctcata tcttcctggg cttcttcgtc
    98041 aagcattgct acaagcattc cggtatctgc aaagtaaatc ttatactttg tatcatcgta
    98101 gtttccacgc agcggtaatt cgggaaaatt caggcaatag cagagattta taattcccgc
    98161 atcgtctaac cattcaatgc agccgcgata atcgcgaaaa cgtgcgccgg aagcaacttt
    98221 agatatctga aactttttat tatctttagc aagctgaatg ggaatgtgat tgaacacatt
    98281 caatattctt gtctgatcca tacctacggc atattttcgg atatcttctt tgtagtcaat
    98341 gataagctgt ctttgaagtt ccagtgaacc ttcaaatgtt ccttttataa taaattcacg
    98401 aacaatcgca ggcattcctc ccaatataca aaaatccaga aaatgattgt tgtaaaccga
    98461 catttccacc tcattgaacg gtgtgagatt tttcatattt tccagcatac tttcaatatg
    98521 accatcatta tatccctttg cccaaagaaa ttcttcaaaa tccatagagt acatttcata
    98581 gtcggattta tagcctacac tgttactttc aattttatga tagttgattc cgagcataga
    98641 tccgctgcat ataacatcaa atcttccgtc gattttgaaa aactttaatg atgtcgcgat
    98701 atcgggaaat tcctgcaact cgtcgaatat tattaaagtt tctcctccaa taaattgttt
    98761 agacggatcc attctggaaa tattttttat aataccttcc gcgctatatc cgtccgctgt
    98821 aatcagtttg tatttcggtt cttctacaaa gttaatataa acaacacttt tataattaac
    98881 attcccgaat tgtataacgg attgggtctt tcctacctga cgcggtcctt taacaatcaa
    98941 aggcattctg tcaggatttg ccttccattc ttccagaaag ttgtctattt tccttttcag
    99001 ataaatcatt tcactcacta tccctcgcca caattatatc cgcaaaataa ctgaaattca
    99061 accgctttca caaaatatga gggagttttt agcttttctg cacatttcat gagggagttt
    99121 ttagcaatct tcccacattt ttagaaagaa gtgctaaaat ctgtcttccg atcataggtt
    99181 catccgctta aagctatcct taatattagt ttaatttttt tctttaaaaa aggatgagca
    99241 ttcgcccatc cttttgttaa tttatatatt tactgattga ttatccttta accgaaccta
    99301 ccattatacc gcctgcaaaa tacttctgta agaagggata tacaatcaga accggaagca
    99361 tagaaacaac catcatcgct ccgcctaagc ccgactggtt gataagaagt tccgtcgggt
    99421 caaatcccgc tgcaaccatc atttcttccc agagtttatc attaccttcg ttcttcataa
    99481 ccgtctcacg aatcttaacc gggaatgtcc attttctcgg tgttctgata tatatcattg
    99541 ccgcatagaa tatattccag aatcccagac cggacagaag acctaatgcc gaaagaaccg
    99601 gcttggacaa cggtagtatt atctttacaa aaatagtcat ttccgaagcg ccgtctatac
    99661 gtgccgattc ggtcaattcg ttgggtatcg accagtaata gtttcttaga agggtcatgt
    99721 tccatcctga agcaagtgag gggataataa gtacccagag ggtatctatc agtcccaatg
    99781 ctctcatgag aaggaaactg ggaattgctc cgccgcctat aagcatgggt attataacca
    99841 tgtaataagc gaagaaatct tttccgggaa gctctctttt actcattgta taagcgccta
    99901 atgcgttaat aagcaatgtc aaaggtgttc ccactatagt tacgattacg gaattcaata
    99961 acggtctgta taccgtctcg tcttttacaa ccattccgta gaataccaaa ctgaagtctt
   100021 ccggccacaa tctcagcgtt gccgtagcag tctcactcaa ctcaaaagag ttcctgaaaa
   100081 cgaataatac cggaataact atgattagtg taaatagaaa cattcccaac aggttaaata
   100141 tattgaaaat cttttctcct aatgatcttt ttaacattta tctcaccttc ttcctatatt
   100201 atcgttcttc cgtcttcagt taagtattta cttgcccagt tggcacttaa cataagaatg
   100261 gttgaaatta tcgagttaaa taaacctgtg gtagtcgaca tgctgaactt tccttgtctg
   100321 ataccggtat tgtaaatata tacatccaaa accattgccg tttcgtcaag caaagcatta
   100381 ccgtccgtca atgtgaaaac aggttcgaat agtccgccca taattcccga gaaagagaat
   100441 attaaaagta caactaccgt atttttaata ccgggcagag taatatacat tgtctgctgc
   100501 catctggtag caccgtctat aaccgccgct tcatataatt cgggatccac accgcccaag
   100561 gatgcaaggt atatgatgga ggaataacct acacccttcc atattcttat accggtaagc
   100621 aaccagggaa ataactgagg tttcccgaga aagtatatcc tttctcctcc cagtaaagct
   100681 ataattacgt taacatatcc tgtactgggg gataacagct gtataaagat accgctgaca
   100741 acaacccatg tgagaaagct cggtaatgtg gaaactgtct gaacggtttt cttgaaccac
   100801 atgattctca cttcattcag caataatgct aaaataatcg gtgacggctt tataaacaga
   100861 tactgcaatc cgataaatac ccatgtattc ctgaatgcct gccagaaagc tctcaagccg
   100921 aacagtttgt caaagttctc aaatccgcac cagtacttaa atccaccggt agtgaattcc
   100981 tgaaacgcaa tcataatacc gggcatagtt acataacgga aaatgtagta cagtatcagt
   101041 cccggaacta tcatcaggta aatcattata tttaccttag tgaagatttt tttctcattt
   101101 tttttgtaaa ttcttaagct actctcgtcc aattacaaca ccaacccttc ttttaaagat
   101161 aaatcccttg aataatcgtg cattataaca ttaatagttt atcacatatt taccgttttc
   101221 aatacatgaa aattataact tttgttgtat atatgtgatt tttcatattt tttggttagc
   101281 aaagcctttt aacaattcaa tacaggcttt gacatcattc atgtccacca ttccgaccgg
   101341 tccgtgtaca tatctcatgg gtatggatat gccgcccgtc cttactcctt ggttggatgt
   101401 atatatggct cccgcatcgg ttccgcccgc cgcaagaata tccgactggt tggctatatc
   101461 attctttttg catatatcct gcattatacc gattagtgat tcgtcacata tcaccgacct
   101521 gtccatgacc ttgattgccg ctcctccgcc caataccgca ttgccttctt tggtaatagg
   101581 agtatcgaat gaagatgtca catccactgc aacagctatt tccggctgca ctaccctact
   101641 tgctactatt gctcccttta agcccaattc ctcctggctg gtaaaaacat aatacatgtc
   101701 gttgtaaggc tcggtatttt ccgttaatgc ctttatggca acataacaac cgatacggtt
   101761 gtccaatgct ttggcaacta ccctgtcatt cttaagcata cataaaggtc cgtaataact
   101821 gcccatatct cccggtttga cataattcat ggcatcttct ttattctttg ctcctatgtc
   101881 tgcaaacata tccccgacat tgttgttttt aatactctcg acatcttttt tgaaacataa
   101941 tacgcccacc gttccgtttc ggaatattat tctgtttcct atcgaaaccg atacgggaat
   102001 accgcctaca ggtctgagat atatatatcc gtcggaatct atatacatga ccataaatcc
   102061 tatctcatcc ttatgggcgg aaaccattat tttcttcttg tcttttccgt acccgttctt
   102121 aacggctata acattatcta ttccgtcggt cataacccgg tccacatgat cttttaccag
   102181 atcggttatg acttgattaa tttcgctttc tcttccgctc actccgaatg tcgcagtaag
   102241 tttttctaaa atctccacaa ttttcccctt tcgttatctc ttttaatttt cggtcttcct
   102301 ttctttggct tctctgaaaa gctttacggt atctttcatg ttgaacagta taagtcccaa
   102361 cagaagtatg ccggacaaca taaatatcaa ctgaaactga ggcacttcaa atacgccgaa
   102421 cggttgcata tttacggcaa taaaatcctt cattaaattt cctatagcgg gagctaccgc
   102481 taaaattaat gcaaccagag cggtatagaa accgtcatac agcgatcgat cttctgcggg
   102541 aataacatca tacttggctt taaacactac cagagaaaaa agcggtacgg ccatggaagc
   102601 gaaaatatta accagtatcg tcataacata ccttgatgaa atattggtaa atccccacaa
   102661 aatagcctgt attaaatata gaatgtaact aacaataaga agaaaatccg tattatgtct
   102721 gtccaccaac ttacctacaa taggatacca gacaaattgc agtataacgt tcaatgtagc
   102781 ggagattgat ataaaagtgt acgacaaacc gagataattc aactggtaaa gtgttgcaaa
   102841 agacatggac aggtaccata tgaagtaaaa cacggcaatg gatgcaatag atttcataaa
   102901 tgtcctgtcc tttaaaggga caatcagcac atccttaagc tttatctttt tcttaggcgg
   102961 ttgcagaggc ggctcgtata ttttatagaa tgaataattt tccgcccaca tgacaatcca
   103021 ggatatagta aacacaataa caatggccat gtactggtcg gatgaggtat ctataaatgc
   103081 cgatacaata ataggaacaa gcagatgagc tatcaaagca atcatattcc ttacgctcat
   103141 atactgacct cttatcttaa cgtctataag atccacaaac cagctgttta tcgcaatacc
   103201 catgaaatta ttaagagcat atgcaattaa agctatggct atcataatgt acgaggcata
   103261 ttgattggga aatatcaacg gaatccatgc tataaagact atcagcgttt taagtatggt
   103321 attgagtatc agagtaaatt ttttacgatt ctttatcgtt tgcatgacat atccggcaaa
   103381 aatagccata acacctgcaa cacttccgat aataggcaga taagctatta ctatttcagg
   103441 agcattaact ttattgaaaa gtccggtcag ataagtggct ccggtaagca gtgcgaaaac
   103501 ggtaccgaca aatccctgta ctataaacca gtctctgtcc ttcttacccg gcatggtaag
   103561 caactttgtg tcgtttctgt agaataccgc aatatataac tttcgtaagt aatcttttat
   103621 tttaccgaac atatcagaat tcctgttttg tcattatttc gggcccattg gctgtaatgg
   103681 cgacggtgtg ttcatactgt gccgaaaggg atccgtccgc agtgtatgcg gtccagccgt
   103741 tgtcctcatc tacataaagg tcataatctc cttcattaat catgggttca acggtaaaaa
   103801 ccatgccttc aactattctt gatcccctgc ctatctgtcc gtaatgaagt attgcaggat
   103861 cttcatgcat ggctctgcct ataccgtgtc cggtaaaatc cctgactacc gaaaagccgt
   103921 tcttttcggc atgcctttgt atatggtaac ctatatcacc cagacggttg ccttctacag
   103981 ccttgcttat gcctttgtaa agacattctt tagtaacggt cataagtttt tgtgcaacgg
   104041 gacttacctc tcctacggca taacaccatg ccgaatcggc catccaaccg tctttatcgg
   104101 ccaccatatc cactttaaca atgtcacctt ctttgagaac atatccggaa ggcattccgt
   104161 ggcatatctc atcattgacc gatgtgcacg aagcataagg atatccgtga tatcctttct
   104221 gagcaggtat ggcattgtgt aatcttataa attcctctac gaatctgtca attgcaaaag
   104281 ttgttatacc cggctttata atcttcctta aattttcatg acaagccgca actattttgc
   104341 ccgcttctct gatcatttca atctgtgact ttgtccgaat attagtcata caaaaatccc
   104401 ttcatccccg aatatatcat tactgcactg ttatatcaag aagtgtacat tcatttattt
   104461 cgaaatagct caactgcatg gatttattca aatctttgag agtatccaac agcggttgta
   104521 tttccttgat aagaataaat gcttctaccc gggtggctcc ttcggatatg ggcaggtaga
   104581 caatctgtcg gaaatcctcc ttgttaaata ccgcatttcc ttttcgttta tagtagaaat
   104641 cctcataccc cgttgtcggg aaaagtattt gatcttcaga aaaaatatga cttttttcca
   104701 gatattcttc gggaacatta cagtaagcat agaaaatcac ttcatcactg tcaaatgccg
   104761 tatcatcgaa tgtgacgtca acataagacc atcttccgtc tatgttcaca acattccagg
   104821 tgtgtgcggt cccttgcgct tctccgctta ccttctgaca ccttataccc gccatattgg
   104881 tcagcatata aaatgcatcg gtgtatccct gacagttggc aaagcccgtt gtaaggcaat
   104941 atgcaccggt aatattgtat ttatccgcat cgttgtcggc ttctttttgg taacccagag
   105001 tttcacatat gtagtcgtga ataattattt ccttatcata atcactcatt ttgctgtcca
   105061 gacgattgtt tacgaaatcc acggcaaaac gataaatttc caattctttc tgactgagca
   105121 atgatctgtc attattgaga tatgcatata caatactgtc accgggataa tattccaact
   105181 catatattgc aaaatagctg ccgctgtttg tcgaataaat cgtcctgtag catcctttta
   105241 atccatattt gtccaagcaa tcaagagaag aaaaattatc ctgtgatgac gaacggacaa
   105301 atatgcttct gtgttttttt gcaacacagg aatcaaaatg gatataaaat tcatcaatat
   105361 ctgcaacata ttttgcatga tcggtattga catcttccaa acaaattcct actgaatagg
   105421 gattaaacga aattttttgc ataaaaggac tgttacccgg tgttgcatca tttttcttta
   105481 tcaaagttat taatgcacag gatgtaagat ttatggcaag taacagtaaa agcactgcca
   105541 tggttatttt tctttttcta cccttcaagc aaatatgcct ccataatgtt aatgaaatta
   105601 tacaacacct ggaaaaagat ttaaacataa taagccgttg tttttattaa ttactataaa
   105661 tacttataca tcggcacatt caagtaggta ttcactcatt ccttattctc acacaatgat
   105721 atataatata ggttgtgagt aagtaaataa tccggaggta tattttatga ttctaaagaa
   105781 agactgtaaa tacgatatta tggccgttac aagcatgggc atcagaatta ctcccgtcaa
   105841 tcgccagcct gtaaacgtat ccaacctgta tgaaatgcat gcgacaagtg cggaaaccaa
   105901 tgtattaaac atttccgcgg ctttaggact aaaagctaaa gttttaacaa aattcgttaa
   105961 ggacagcccc attgcacttt atataaaggg agaactcagg aaaagaaata tagaatttga
   106021 agggattgat attgaacagg gaggtccttg gggatatcgc catcaattta atatagccga
   106081 cagcggtttc ggagtaagag gacccagggt gttgaatgac agagcgggcg aagtgggaag
   106141 aaccctgtcc gcatcggact ttgatctgga aagaatattc aaagaggaag gttgcagaat
   106201 tttgcatctt tcgggattga tagcggctct ttccgaagaa acatcgaaat gctgtctgga
   106261 tattgcatta gctgcaaaaa aatacggaac cgcaatatct tttgatttaa actacagagc
   106321 ttctttctgg aaaggaagag agaaagagct acgtgaaata tttacaaaga tagcttccat
   106381 atcggatata ttgataggaa acgaagaaga ttttcagctg gcactgggtg tcaaaggacc
   106441 ggaagccgga ggtaaatcaa tttccaaaac cgacgcattc aaagatatga taacaaggat
   106501 atccgcacag tttaaaaatg taaaagtatt tgcaaacact ttaagacaag tggaaaatgc
   106561 aaacttacat ttatggggtg ccatcatata tgccgaaggt aactggtatg ttgtggagcc
   106621 gcgtgaaatt cccgttctgg acagaatagg aggcggagac ggatttgtcg gcggtctttt
   106681 atacggtatt cttaaaggat ggcaaccgga aaaatgggta cagttcgcat gggcaagcgg
   106741 agcgcttgca accactcttt taacggatta cgctcatccg gcggatgaag aagtaatatg
   106801 gagcatatac gagggaaatg cgcgtgtaaa aagatagaag agatatcttc ccgatactct
   106861 tctatttagc ggcataatgc cagatggtat ataaaacaac ataagacctt agacggatac
   106921 atgtagacta caatttaaac cggctaatat gtgctgtcct ttgcaacttg ctttctgagt
   106981 ctgttcagtt cctcagtcat ttccgtaaat gttgcaatac tcattattac caattcacca
   107041 tatccgttct ttgtaacaat gataggctct tttgcagctc tgcacatctg cagtacatgt
   107101 tttgtatctc gtaattctgc tatgggaaca atcttcggca cgttcataac agtacatcct
   107161 ccatattagg atagtatact atatactaaa taatgtcaat gaagaaattt gaaattcatc
   107221 ccttaaatat gcaaattatg tgcatattta agggtgtttt tatgacatct taaagaaaat
   107281 tatccgttac tttctcttaa gattacttgt cttatattcc accaatactt ttaagtggtc
   107341 cgacagtgcc tgcatctccc atcgggcttt ctgtccgtct cttgctttta gtgactgata
   107401 gatattgctg tgcattttca tacattgctg tttagcaccg gcatcggata catatatatc
   107461 ggaaactgca aatatcgcct gctccaatgt cataaatatt tgtgaatatg ccatattacc
   107521 cgatatatcc gctatctttt tataaaactc atgatacaaa gcggcaagct ttttctcatc
   107581 tttcagtaaa acttcctgct cagctatgtt tcccaactga acaagctctt cctctgttgc
   107641 ctttaatgcc gcttcatata cgccgttaca ctctatgaat gcaaagatat caaaaaaact
   107701 gtctatgatt tcctgttcca atttaccgaa tgcaacagca caattcaatg taagaagggt
   107761 gccttgcttt ttccagttgc gtaccctggc accttttctc ggtactatgt ccacaaatcc
   107821 tttagtatta agaaccgcca cagcggtgtt tataacactc cttgacacat tatactcatc
   107881 acacagttta cgaaaagagg ggagcatagt atttatctta tactccccgt ttacaatctt
   107941 cttttccatc tccaggataa acctttcggt taaactatcg ctcccgttca ttcatcctgt
   108001 tccttctccc atttaagtac gggatttctc gctgcagtta cctcatccag tcttcttaca
   108061 tatgtgttat gaggtgcatc atgcagaagc tcgggatttg tttttgcttc ttttgctatc
   108121 ttattcaatg cttcaatgaa tttgtccagt gtttccttac tctctgtttc ggtaggctct
   108181 atcatcatgc cttcatgcac aatcagaggg aaatatattg tcggcggatg ataaccgtaa
   108241 tccaaaagcc tctttgccat atccaatgtg gatatgccgt attgctcttt ctgtctgctt
   108301 cccgaaagca caaattcatg catgcagaat ctgtcatacg gaatgacaaa actgtccttt
   108361 attccggcca ttatatagtt ggcattcaac actgcggttt cgcttgcctt tttcaatccg
   108421 tcgggtccca ttgtcagtat ataggcaagt gcctttatgt acacaaggta attaccgtaa
   108481 aatgctttta cacgacctat cgcgtctttt ctgtcatggt ctaaataata tcgctcaccg
   108541 tccttgcaga ctaccggttt cggaaggaat ctgaccaaat cttctcgcac acaaaccggt
   108601 ccgcttcccg ggccgccgcc accgtgaggc gtggacattg tcttatgtaa gttaaaatga
   108661 acaacatcaa atcccatatc aaacggacgg gcaataccca ttatggcatt aagattggct
   108721 ccgtcatagt atagtaaacc gcccttgtca tgcacaatct ttgctatctt tgtaatattg
   108781 ctttcaaaca accccaaagt attgggattg gtcagcatca gtcccgcggt ttcatcatcc
   108841 attaaggcgg ccaatgcatc aatgtccacc agtcccaaat catccgattt aacctgtact
   108901 atctcatatc ccgccgttgc cgcacttgca ggattggttc cgtgagcgga atcgggaact
   108961 atcattttgg ttcttttatt gtcattcctg ttctgatgat aagcctttat tatcattata
   109021 ccggtcattt caccgtgagc tcccgcagca ggctgcaacg tagcaccttt catgcctgtt
   109081 atctcacata aatacccgcc caattcatat gccgctttaa gcgcaccctg aacctgatat
   109141 tccggctgat aaggatgtaa tgaagtaaat ccctgaaggc ctgcaacatc ctcatttacc
   109201 ttggggttat acttcattgt gcacgatccc aaaggataaa atccctgatc cacaccgtaa
   109261 ttatttctgg aaatgttggt aaagtgtctt ataacatcca cttccgacac ttcgggaaga
   109321 gatacatcgt cctcccttgt gtatttctta tctatctcat aagacggtac ttggctttca
   109381 ggcagtgaaa atgcgcattt tccttcttta gataaattaa atatcaattc agccattaaa
   109441 ctcacctgcc cttcttatga actcatccat ctcttctttg gtcctttttt ccgttacggc
   109501 tataagccat cctctgtcgg tctccaagcc ccctataaat ccgtgtccgt aaagatatcg
   109561 gttcaaagcc gctatattta ctttcggttt aaccgtaaac tcatggaaga aaggcatatc
   109621 cgaagtcttt tcaaacttac ctgttttcag cagcccttca taaacataat gtgcattatc
   109681 cacacagcgc tgtgcgacct gcttaaatcc ctttttacct gtcaccgaaa gataaatggt
   109741 tgccgtcaag gcacataatg cctgattgga gcatatgttg gaagtggcct tctccctcct
   109801 tatatgctgc tcccttgcct gaagggtaag cacataagcg gtttttccgt ccacatctgt
   109861 agttttaccc actattcttc cgggcatctt tctcatgtgc tctttaacac atgcgaaaaa
   109921 tccgagataa gggccgccga atcccatggg cattcccaga ggctgacctt ctcccactac
   109981 gaaatccgca ccgaaatctt taggcgattt cagcagtgcc agcgccacgg ggtttgcaca
   110041 gacaataaac aaactgccgt tttgctttac atagtctccg attacttgca tatcataaat
   110101 atctccgtag aacgacggat actgcacaca tactcccgct acatttgtta aatcggcttc
   110161 caacagttgt ttaaccgtca tagatgtgta ttttatatct ctgaatttca gatatgtttc
   110221 cacagtctct ctatactccg gatgcatatc ctccacaatg attatatcgc ttcttttacg
   110281 gaaagcaccg gaggatattg cacacgcttc cgccagagcc gatgcaccgt cgtacatgga
   110341 agcattggca acgtccattc ctgtaagttc gcatatcatg gtctgatatt cgaagatagc
   110401 ggtaagagtt ccctgactta tttccggctg gtacggggtg tatgccgtta agaactcctg
   110461 acgtgatata aggtgagata taaccgaagg tatatagtgg tcataagcac cggcacctaa
   110521 aaaacatgca taattgccgg tgttgctgtt ttgcgcggcc aaagactgca tgtattttat
   110581 aagttccatt tcgctttttg ccggcggaag attcaaaggt ctttttagtc tgacttgctc
   110641 gggaatacaa gaaaaaagat gctcgacact gtcggcctta catcttttca acatttcctg
   110701 ccggtccctg tcagttaccg gtatatatct cattattccg cctcgctttc gaggaactcg
   110761 ttatattcat cttccgtcat aagatcattc agttccgttt catcatcaat aattatctta
   110821 gccaaatatg tgccgtacgg atcttcattt atcttttccg gagcatcttc cagctcggca
   110881 ttggcttcaa taaccgtccc gcttaccggc atatatactt ctgcagcggt ttttaccgat
   110941 tcaattgcac aaagaacatc accttgttgc agcacgagat cttcttccgc caactcgaca
   111001 taaacaatat ctcccaactg ttgctgggca tagtcggtta tgccgatata cgcatatttg
   111061 ccttcttttt taatccattc atgatctctt gtaaatctca tatcccactc cttatcattt
   111121 tttatacatt tttttataaa acggcttctt taccgttact gctttaacct tttttcctct
   111181 tatgtccaca taaacgaagg taccgacttt agtatattcg gtggaaataa gagccaatgc
   111241 acagttctta cctaaactcg gacaattact gccgcttgtc acataaccta tcttttcgcc
   111301 ctcttcattg aatacgtcat agccgtttct tgctatacct ctgtctacca tttccaagcc
   111361 tgcagaccga acggacacac ctttttcggt ttgttcaatc agagcggctt ttccgataaa
   111421 atcgcttttc tccggtttaa caaaactctt cagtcccgca ttgagcggtg ttatctcttc
   111481 ggacaactcg tgaccgtata aaggcaatgc actctcaaac cgcaatgtat cccttgcccc
   111541 caaaccgcac ggagcggcac ctgattccat gaattttctg aacagtttct cagtatcatg
   111601 ggcggccgta tatatctcga atccgtcttc tccggtatag cccgttctgg ataccatggt
   111661 gttcacgccg tctacaacca cattattctt aaatctgtaa aatgcaatct cttccagatc
   111721 ggtcttagtt aactgctgca aaatattttg cgcttcaggt ccctgcagtg caatctgtcc
   111781 gtatctatcc gattggttac tgacaaccac atcataccct tttgtatgtt ttttcatcca
   111841 ttcaaaatcc ttgtcggtat tggaagcatt tgcaaccaca agaaaatctt tatcgttgtt
   111901 cttgtatatc agtatatcat ccacacaccc gccatgttca tagcacatgg gtgaatacat
   111961 tatctgattt tccgatatga cactgatgtc gttggttata atgtagttca aatactttat
   112021 cgcatctttt cccgttaccg aaatctctcc catatgcgag acatcaaaaa tacccgcctt
   112081 gtttcttact gcattgtgct cctctatgat ggaggtatac tgcacaggca tatagaagcc
   112141 cccgaagtct accattttgg ctctcaaaga ttcatgcaca gaggttaaag gtgtcgtttt
   112201 cataaattat atgctccttg aaatatatat ttataatatg ttctaaaaga agaaaagtca
   112261 acggtcatag cctttatctt cttttcagtt tctcatacac ttcattataa taaaggtaat
   112321 tctcgacatt gtgcccggga gttatgtggt tgcctattgc aagcatgtat ccggggcagg
   112381 gcttacccaa atccatgcac cgtttgacct cattttcaat gtcttccctg ctgcctctca
   112441 ttattatcct tgtatcaaca ttccctataa tggaaacggt tttcccgtat ctttcgcaaa
   112501 tatattttaa gtcggtaaga ggttcgaata tcagacaatc cacccccata tccacaaacc
   112561 tgtccacaaa caaatcaaac ccgccgtcac aggtaaataa gaccttctta cccgcttctt
   112621 tcaaataaga gatatttttc ttaacataag ggaatacata tttgtcatac caggcaggag
   112681 ctatgaaagg ccccgacgac cataccatat catcatgtat catacatacg ggaacatcgg
   112741 atttggccag cgccttaaag tactgtgaaa tccattggga atatcttacg gtaaactcac
   112801 cgaaacgggc aggatcttcc cctgcagccg acagtagcat atcccatccc agtatctcta
   112861 aaattcccga tatgcatgtg acatatatac ctgaagtatt aactccgtca ggatgcaatt
   112921 gacatctttc aagataaccc tcggttatcg cttttgcttt ggtttttaag tctattgttc
   112981 cgtatgtttc ataaaaatcc agattatata tttcctcaat atcggtaaag gcctcatgct
   113041 tattatccct gtaatccccg taaccttctt caaatcccgc atgccccata gtggtccttt
   113101 tatctccgaa gatattactg cctattccgg ttgcccagaa aagtgatata tcccatgctc
   113161 tttcaaaggc ggtaattgca ttgaactttg tatcatcatc ggaatcctta ttgacggcta
   113221 ttcccgtaac cgcatgtaaa agttcgtatg ccaactgata agaatattcg gttcttggga
   113281 ctatatcggg catttccagg ttaacagcct gcattcctaa ctcatatgac atacaaaatc
   113341 ctccataacc tattctatca ttattactga tgtttacaag gtataagcat ttgacttgat
   113401 aagggtaaag atataatatt ttatggaaag actgatgttt ataagttact taaaagtacc
   113461 gacacttatc agtaacaaac gatataaaag aaaaggagta tgtgacatga caacattgat
   113521 agcaggactg ataatctcaa tagctttatt aagcgtcata gtttatttca acagcaaaaa
   113581 ggacagcaaa aagaaattcc gtgcaaactg tatagttgcc gcacttccgc tcataatagc
   113641 atttttcatt gcctcaatag cggtaattcc ggcaaactcg gtaggtgtac aatacagccc
   113701 gttcaaagga gttctggagg aaacacttcc cgaaggatgg cattttaaag gcgtattcga
   113761 caatatatat ataatcagta ccgaagtgca gaccagcacc ctcactgaaa ttaccggtca
   113821 gacaaaagac agccagtacg tcgaaatggt tattgacgtt aagtataagg tttctccgga
   113881 aaaagcctat gaagtgttta aacaatacag aactttggag aatgtcgcag taagtctggt
   113941 atcaccgacc gtacaaagaa gtattgaaac catttccata caatacaaca ttatcgaaat
   114001 attgggagaa aagcgaaatg aactttacgc aggcatcgaa gaggaactta caaaacgttt
   114061 tgcaataaac ggtatacagt tcgtatccat taactttacc gataccgatg ccggagcgga
   114121 aattgaaaaa gctattcagg atgaagccat cgccaaaaaa cgtgtggaaa cggcggaaca
   114181 ggaacgtctg cgtgtggaaa tagaagccca gcaaagagta atagaggcat cggcaaacaa
   114241 ggaaaaggcg gaaatagagg cccagattaa actgattgaa gcacaggccg aagccgaggc
   114301 caacagactt atagccgaat ccataacaca ggaactgttg caaaaaatgg aaatggaagc
   114361 ccgtcttaaa tggggatggg taaccgttca gggcggttcg gtaataacgg atataaggga
   114421 gaagtaataa tccgctattc aagaatcgga atttgttaag gagaattgta tggagttcgg
   114481 atggattaat gtgttcggag cgggtattgt gctttttctt atgataccga acattgttta
   114541 tgccgtaaaa aacaaaaatg agaaaaatgt ttgtaccgat aaatatatga acataacgga
   114601 gcagatagga agatatgctt gcattatcct gatgtggctg cctcttcttg tttggaaatt
   114661 cggatttgca agtgtatttg aaatgctcct ttatatcata ggtaacggcg tactgctgat
   114721 tgcatactgg tcggtatttg cactatactt aaaaaagaag aacaaaaacc gtgcacttat
   114781 cttagccata ttgcccgcat gcatcttttt ctttagcgga ctgttattgc gacattggct
   114841 gcttgtgggt ttttccgtta tatttgcaat cggacacatt tatgtaacat tgaaacagtt
   114901 tagtccgaca actaccgtac aaaatgacaa atagaaaaat gaccaaacac caaaccgtta
   114961 ctcatccgtt acaacctctt tttaatgacg actcaaaaat cttaatcttg ggttcctttc
   115021 cctccgtaaa aaccagagaa tacggcttct tttacggaca cccgaagaat cgtttctggc
   115081 ctttgatcga gaggattttt gatgtgaaac tgagtacgga tattgaagaa agaagaacct
   115141 ttcttttgca acatcatatt gccgtctatg atgtcattta tcaatgtgac attatagggt
   115201 caggtgattc cagtatccaa aatgtaatcc cctcggattt aagccgaatc tttaaagagg
   115261 cggatataaa gaaagttttc tgtaacggag caaccgctta tcactactat cagaaatatc
   115321 aggagcaaaa aactaatgct aaagctattc aacttccctc taccagccct gccaatgcaa
   115381 gatattccat ggatgatttg tataatcaat ggaaggtaat aaattatata ctttgacata
   115441 cgtattaata cgtgttatta tatagttgcc gaaaggaatg tgcctgtgaa agtatctgaa
   115501 ctcattaaaa aactgaaggc aagtaagaag tgtcatctca tcgaacacgg tgcaagacac
   115561 gacatgtggc atagcgacat aaccggaaaa aacttccctg ttccaaggca taatagttag
   115621 gagattaatt ctatgaaata tgtatatccg gcaatattca cagttgaaga agatggaggc
   115681 tattccgtaa actttccgga ctttgaaagc tgttacacat gtggcgatac attagcggaa
   115741 agccttgcta tggcggaaga tgttcttgcc tttacactct acaaactgga aacggaacaa
   115801 gctttgcttc ctgctcccgg taaacttcaa gggttaagtc tcggagaaaa cgagttcgcc
   115861 aactatatcg catgcgatac aatggagtac cgaaaacgaa agaatagcaa agctgtcaaa
   115921 aaaacactca caatcccttc atggctaaac gaagaaggaa ttgcaagagg tatcaatttt
   115981 tctcaagttt tgcaggatgc tctaatggag aaaatagaaa aatatacaaa aacttgaata
   116041 gaaatccctc aaacacttga ataaaacttc ctcaaacact tgaatatgtg ctataatgtc
   116101 cttgtttagt gaggtaggct tatgaagaca tatttaccga ggattattga taaattatta
   116161 aaagaaaggt tggaggctaa aggagctgtt ctattggagg gacctaaatg gtgcggtaag
   116221 actaccagtg ctaaagaaat agcagccagc tttatatcaa tagaccagcc tgaaatgacc
   116281 aagcagtatc agtcaatggc tgaactctct ccgttgcagc ttttggaagg agaaactccg
   116341 aggttaatag acgaatggca aatagcacct aatttgtgga atgcagtaag atatgaggtg
   116401 gatcaaagag atgagttcgg tcaattcatt ttgacaggtt cagctgttcc cgcagaaata
   116461 gataagtcta tgcatacagg gaccggcagg atttcaagac tatatatgcg ccccatgagt
   116521 ttatatgaat ctttggattc aagcggtaaa gtatctatta gcgacttgtt tgcaagtcgg
   116581 gaaatatccg ctaaaagcga tacagacttg gaggaaatag catttttaat atgcaggggc
   116641 ggttggccta aatctgtcgg attacctgaa aatccggctc tttaccaagc cagagactac
   116701 tttgaagctg tcgtttcaac cgacataagc agagtggact cggtaagaag agataaggaa
   116761 aaagcaaaaa gaatattgcg gtcatatgca agaaatgtgg gaaccaaaac ctctttagag
   116821 actataaggc aggatgtatt ggcaaatcac gcaggcacct tggaccctgt gacactctac
   116881 tcatataggg acgcattaac aaagatattt gttattgagg actcacctgc atggaatccg
   116941 aatttaaggt ctaaagtagc tatcaggaca actgatacaa ggtattttgt tgatccttca
   117001 atagctgctg ctgcattgga actgggacct gttgatttat tgaacgattt aagtaccatg
   117061 gggcttattt ttgaaaactt atgtgtccgc gatttacgga tatatgcaga ttacttggat
   117121 ggcagcattt atcagtacag agatagcaac ggactggaat gcgatgcggt tattcactta
   117181 agaaacggct cttacggtct cattgaaata aagttgggcg gtgacagttt gattgaggaa
   117241 ggtgtcaata acctaaacaa attggcttca aatatagata cgagaaagat gaaagaaccc
   117301 gcatttatgc ttgtcttatg tgcaaaggct ccctttgctt accgtagaaa agatggagtt
   117361 tatgtggttc ctataacctg cttgaaacca taaaaaacat tcaaataaaa ttgtagaaca
   117421 attggagcag attgtcggtg tctgtgcttt ttgtaccgta ttcttttttt taactaatcc
   117481 ggaataacta cttcaatagt tattgaagtt gtattgaagt agcattgaag taaccattga
   117541 agttgcattg aagtaaccat tgaagtataa cagaagtaaa tcaataagcg gaagttgtgt
   117601 catgcaatat gatttaatgt ctattatttc aaaaagacga aatgagctta tatccgttat
   117661 attggtcaaa tgcaatgtta tggaagcaat cttattcgga gctttgaaga attggaagca
   117721 agaggaaatt ttttataagg caaagtttgc gaaggtagtt atgaggaaga atggtcaatt
   117781 ccaaaatttt aagaagattt tctggcacaa cagaattcac gtaaaagcta tgtacaggat
   117841 gagcatagcg acatttggaa caggggttat tcctaacata tttaaattaa aactgtaatt
   117901 gcaaatgaca agagcacacg attatccgta caatcttgat gtcagacaaa tgccatagat
   117961 tgaaaggatt tatttccaaa ggttttacag atttaaagaa tacataaaaa cagcgttttt
   118021 tctcgacttt tcctcaacaa ccgtatgtct tgtttaagct gttcagagta ctctttatct
   118081 catcaaccag attttccgga gctaccagtt gcgccttatc cccgaatgta aaaagccagg
   118141 ctatgaatgt agggcttatg gaaacattca ggaaagcctt gaatttgttt tttcccacgg
   118201 ggtataccgt tatatccaac ccgaaccggt caaacaccac attcaaaaga gacatatcaa
   118261 aaataatctc ggcttttacg ggctctccct taaacatgtt gaacatgggt tttgtataag
   118321 ttacaagatc aaactcttct tttttatcca tatcaatctt ttctttcaat atctctatgg
   118381 actccatttt atccactttg aactgggtaa cattatatct ttcgtaatag catacaagat
   118441 aatagcactc gtcttcccat atcagggcat acggagatac cttatacttc ccccttactc
   118501 ccctgtaaac tttattctta tcaatgtcat aatcaaagta actgaaccgt attttggcat
   118561 ttttggatat tgcctcgttt atgctgtcta tgttgtaata gacattctca tttttacttt
   118621 tagctttact gtatactccg gtattcctta caagctcttt cttctgatgt tgtgatgcca
   118681 gattgcataa gccgtcaata agctctttgg tcttcttttc ggttaaaaat ctggcagcta
   118741 ccagagcatc gatgataagc ttcaactccg gtatctgaaa ctcccttgat gccatgtagt
   118801 atccgtatgt tttggatttt acacctatta tgtcacatcc caattcattt aatactttta
   118861 tgtcatcata tatgcttttt ctttcggcgt ttatacctac agttgccagc tgccgtatta
   118921 tatcgtttac ggtcataaca tgattttcgt cggtgttttc atacagcatt ttgtagatat
   118981 gcaataattt agctttctga accgctgatt tggccattgt ttcttccccc ttaagctctt
   119041 ttatatatat actcttcccg tctgtttaat aatcttctgt ctttaagcca tgccggtata
   119101 tcggctatgg atattatata cggcatacga tggtgtatgg acataaactg ctcatatgct
   119161 tccgttgtta ttataacaaa cctgtcttct ttatatacac ctgccatgta tataaccgag
   119221 cggtcgggtt ttgtgaacag atatttcttt ttatctttat cccattcaaa atatcccgta
   119281 gcgggtatta tgcacggctt catgtattta aacatggtaa gttcgtttac ggtctcgcct
   119341 cttgcattga tgactgtttt gttattgtac ttattcacca ctccccatat cttatccatg
   119401 ttaccttgta tggtttctat ctccacaagg tcggaaggaa atatttctct ttcttccaaa
   119461 ttcagaaatc ttccgcacat cacgctttcc tttccacaaa ccatttgtct tcttcaaaaa
   119521 atatatatgt ctctgtaccc attatcttac ataaataccg tactccgcaa ccccctgctt
   119581 tgagacttgc cgccctcctt atatccaata ccctgtctac ggtatatatt cttccgtctt
   119641 cccattctat attaaccggt atcatctgtc cgtccttttt aaatttaacg gtaacattga
   119701 catattcttt taccctttta tcttctgtca tcttttctct cccgtgttaa aagaaacttt
   119761 cctcataagt gcttcggtgc tgtcggataa atccccgcaa tctctcccgt gaataagtac
   119821 cgctctttgt acggcatacg gcccgtatcg ctttcttatt gaatctatgg agtattccaa
   119881 gtccattgtc ttgaagcgct tgttttcctc tttaaatatg gatatctgta taggtccttt
   119941 ggaacttgca aggtcggtta cccttatgcc gaaactgcgt accggcttat taaaatacca
   120001 gttggctttt accagtttca tggctctgtc ggctatttcc gaggatatat tggtgtaatc
   120061 ggaaagctgt ccctgtcttt caaacgattc caaatcgcag tcccgtatat atatctgtac
   120121 ggtttttccc tttagtccgt gttctctaag ccttctcgca acactttcgg ccaatacaaa
   120181 agaagttatc tttatatcct ccgttgagca taaatccttc ggaggagtgg tgctgttgcc
   120241 tatggatttg attattgttt catgttgggt attctccacc ggtgaataat cattgcccat
   120301 tgcgtaatct ttaagtatcg ttccgcattt tcccagtttc tcacgtataa atactgcatc
   120361 aatgtcggca agctccccta ttgtatttat attccaacta ttcagttttc ttttagtggc
   120421 atatcccaca tacagaagtt cccctacatc aagaggccat accttatctt tgtaattgtc
   120481 tcggtctata acggtgacgg catcgggttt tttcatatcg gaacctaatt tggaaaaaac
   120541 tttgttgaaa ctgacaccta tagatacggt tatacccaat tcaaatttga tacgtctcct
   120601 tatcttctcg gctatctcgt aaccttgatc cattgttctg cccggaaaag aaatatctat
   120661 ccaggcctcg tccataccga aaggctgaac cttctcggaa taagagcagt aaatctctct
   120721 tgcaagattg ccgaaccgaa gatatctgtc ataatgcgga ggtaccacta caagattggc
   120781 ggctttaagc tttgcctgcc acaccgcttc tcccgttctt atacctacag cctttgcctg
   120841 ttgatttttg gcaagtacta ttccgtgcct gttctccacg cttccgcata cggcaaccgg
   120901 cagatttctt atggcagggt tatacagaca ctccactgaa gcataaaaac tgtttaagtc
   120961 cgcatgcaaa ataatagttt ccataatacc cctaccttca ggcgtattat ataatacgaa
   121021 catatgtttg taaagtgttc gcttttaact gtccgatatt ctacccatat gtccgtttta
   121081 cagaaaagtc attgaaagta caactttatc actcgattat gtattcccac tttatctgat
   121141 ccttgtcttc tatttcatac gtgtcgctgc ctacggacgg cgatacaccg ttgacataat
   121201 aaatccatcc gctggtagga ccgcaatcaa attcatatag cccggcaata ccttcaacat
   121261 aacgtgtaac ctttgttcct cttgacgaca tgtgtatttt attatcttta catgccgcca
   121321 atgtgacatc gaataccgtc gtgccttctt tcagacttac cgcatattcc gataagatta
   121381 ttcctttatc cgctttggtt gcatctattg tcacatatac atacccttcc gaaagatcgg
   121441 tatcttttgt gcaaccggtt attaagacaa atattaagca taataaaaaa ataatgtata
   121501 ctgtacgttt cattaataag attctccgta aaaaatcttc accatcaacg atatggctgc
   121561 ttctcttgtt ataaaactat catcattcac tacatcaata cctgctattc tgctaatact
   121621 ttctgtcagc tcaaaaaccg tgatatatga gtcgggttca aaatatactt catccgctcc
   121681 gaacattatt ccgttaagat agcaggctaa aacatacttt gaataccaac tttctctaca
   121741 aatatcctta aatctgggaa caaaaatatc ggtatctaac ggagcaacat tgtacagcat
   121801 aacacatgct tcggcccggg taagattatc taaaggtcgc aaattgtttc cgtcacctac
   121861 aaccaacttt tcagtatacg ctgtcaaaac atcgctataa tacctctttg aaatactttc
   121921 ctcatcgaaa aatcccgtct tccttactac ggccggatct gtcggttcat ggataatatt
   121981 cggcggtact gtcgaagaag gagatgcaat cggtgaaggt gatggagaag gttcggggat
   122041 aacactcttt tctttcttta catatacata accggttttc attgcactta atgccaaata
   122101 tgcctgttcc gtcgccatag catcggcact ttgggaaata ctgtgcttga aacgaaaatc
   122161 ttttgtcata taattttcta tcaaagtatc ataaagtgtt ttaccgttgc gaacaaatct
   122221 gctatcgttc aaggatatac cggcggtaca tagagcaata ataatctggg aaagtatttc
   122281 cgagctgtcg aacttctgat aatacataaa tccgtcattg tccagctttc cgcttaaata
   122341 ggataaagct ttatccatgg cttcttttgc ctgcttattg ttctgataat atgctaatac
   122401 ggacacagcc attgcagtta aatcactatc cgaaccccac ccctttgcca aaggaaaacc
   122461 gccgtcctca ttttgatatt ccaacagcag agtcaataaa ttatccttgg tccatattgc
   122521 attgtcttcc gattgcacat cggtcaatgc gaaaagggcg aaaataacgc cgtttactcc
   122581 cgatttatct atcttcttaa aagactgaag cagagaaaca aggttatatc cgtgaaaatc
   122641 cttgggattc tttccgtcct gctttaaaag aattattatc ttggcatact ccaaagatgt
   122701 cctgtagact ccgtttctgc tcacaatatc ttcttttaat gccgcataat atgagtccgg
   122761 tattctataa ccgttaagtg aaagtccgag agcttcccat aaggaaagct ccgctttttc
   122821 aaggtatagc tgtgatattt tttcacatgt atcttccgaa gctttaacac atacagctcc
   122881 gagcaaaaca catacagcca atatcacagc cagagccttt ctcatttatt tccctatagc
   122941 ctccatatag cttattatca ggtaggcagc ttcttctcgc gttaaacctt cagtaaatac
   123001 cgctttctgt gcatcactca aagcggcatc cgatgcaaat gccgtattca gagcatactc
   123061 ggcaacatcg gctgcaaccg cagcattggg ataaagatat cctttgtcat gctttgccag
   123121 accgtatctg aataccgctt gagcataacc tccgaaccag ttgcctgcaa cgacatcgac
   123181 atatctcttc ctgtatttac ccggtacatc cagtttggat gcacgtacga gcattgtaag
   123241 gaattccgct ttggtaattt catcggcagg tctcagatta ttatcctgat ccccgttcat
   123301 gatatattta cctaccgcct tttccatgta tgttaagtaa ttatcggtta cttccgcata
   123361 atcgttgtat tgctccaaag atacatcgct gtcacttgtt acatcaacgg tcaccgacgc
   123421 tctggaaata ttgtcttcct tgggtaatac ggatatttca taaacgcctt ttttgttaaa
   123481 cttaagattt acatatccgt cgttatcggt tacatatccc gctttatgtc cgtttaccgt
   123541 tatctcacag tttgcataag gatgaaccga tgtttcccat gtgacaaaat ccagtacatg
   123601 gcgatacaat ctcactcgta tattctcacc gccttggact tttatgcttg tcatatcaaa
   123661 gtaagaatag gtatcggttg cccaatcgtt taaaagataa atatccagaa catctccgtc
   123721 agtcaacggc tgttcattta ccgcaaacca ggatgtttgg ttatttaccg tatacatcca
   123781 ggcggaattg cccgaggctt ccactcccgc caatgcggat atgaaagaac ccttgttgta
   123841 attagcaatt ttaggatcgg ctgcatcaaa accgttactg ttgagtgccg caattatggc
   123901 atgtaaagcg gaaaattcac actccgatga tgcatcgtat tttgcaagat caaacccttt
   123961 aggctccaca cacgctctgt aggttttatc ggttgtatat atgtttaccg ccacaccttt
   124021 accctgcaca tctatgtatg cataggggcg ggagatagtg tttatcccgt caacatattt
   124081 ctcggcggat actatatgag tgcctttatc tttgaatgta aggtaggcat tgccgaattc
   124141 atccgttacc gcatcggtct tctttccgtc caccgttata accgctcctg caagattctc
   124201 attagaagca gcccatgtaa cggggtcggt tacctgatgc ttaaggttga gctttacggt
   124261 ggtaaatgcg tcaacttcat actcgtccgc tacaccgaat gtggaatagg agcagttcat
   124321 ccagtctttg atataataga gtactacata atcatctttt tccagctttt ggtcctttac
   124381 ggaattccat gacgattcat cgttcagaac atacatccaa ccggcatttc cttcggcttt
   124441 aagccccagt accgaagaaa tgtaggaacc gccgtttgct tccaagacct ttgctttgga
   124501 cgggtcatgc cctttttctt tcaaagcata tattaccgca tgaagcacag tataatctga
   124561 aggctcttcg gtaactccgt atgcctttaa atcaaacctg ttcaaaggca ccgctgtctt
   124621 ttccactaaa gtgccttcat atccttctat gcgtatgtat gcatattccg tctgtgcagc
   124681 gaagactgca ggtgtcattg caataagtaa tgccgttatt actatcatgc agatcgtctt
   124741 tgtacttctt ctcataattt caatcctccg ttaaaattaa aaaatcctct tcttgcagag
   124801 aaaaggatta aactacattc cccatcttcc gcaagcaatg tatgctatat cctacggcag
   124861 gtcttctgac tgaaattcat cgcttttgca ccttcccgta tctctacagt ggtttattgc
   124921 aatcgctcct tcacacagcg gcgggaccgt gcaggatttt cacctgcttc cctattaaaa
   124981 aattgctttt accgtaaaaa tcgcatattg agttgtataa ttagttaatc ataaaagggt
   125041 agttgtgtca accgaatcaa gccttttccc gacttgtttt tttgatgctg atactatcag
   125101 tacaaccaca attactaccg ttacagccaa aagaaatatt gtgccgggaa tatataacct
   125161 gcacagcata aagtatgaat agattgatag gataacggct tcggtaagcg ccgacacaaa
   125221 cttgttttta ataaacaggt caacagtcat ccggtttaat gcaaacacaa aaaacggata
   125281 gataacgtac ggcagatact cggtatagtc ggcattaata agggaggtta aagcgggcag
   125341 tactttattt gttatcccta cgtattcaaa acctccgaga ttgattgcaa cggcatatcc
   125401 aagacctata atcgttgtgg gtatgcccac atatatgcac tgaatgaccg attcgcctgc
   125461 tgaaaaagcc gctttgtctt taatacctat ctttatatgc agagcatatc ttgtaaccat
   125521 atgtattaaa gcacttgtaa taccgataaa aagagaataa acaatataat cggaagacaa
   125581 ccgtgtcttc aaaaaaggaa cgaggaaaat aagggagaat ataagatttt tcactactat
   125641 tccgtaaatg gaaaaactcg ccatacctaa aagcatggac tttgtttttt tccgtataat
   125701 catgacaaca gaaaaaacaa ggggcagaat tacaaatgcc cctgcagata ttgccatgat
   125761 aataatgtta atacggccta ccataattta tccgaaatac tcctgcaaaa acagagctat
   125821 ctgctcatcc cagcgctccc aatcatgggc tccgccgtcg gtagcatatt taaggtttaa
   125881 tttcgcttct tccgcacact ttttgaaact catgttgtga aggtaaagaa aatccgatgt
   125941 cccgcagtac tgataaacca aaaggtcttt tacggaatat ccgttaaccg tcttgtttct
   126001 ttgtataagg gaaataaggt cgttgtcggt tccggcaagt acggggtctt caccgaaaat
   126061 gcttataaac aactgtatat ttttcctgtc ggtctgctca tcgcctgtaa caaaaccttt
   126121 aatgttcgcc atatccaaca ctccggaaaa gcttcccaca atacagaatt tgtcgggatt
   126181 attaaatgct attttcaaag caccgtatcc gcccatagat aatcctgcaa tcaaattgtc
   126241 tttcctcctg gaggaaaatc cgaatgtgcg ttgaacatac tccataaatt cggtagtgag
   126301 atatgtgtag aacggctcac cgtatcgcat atcggcataa aaactgtttt ttgcgttggg
   126361 gcagaccacc gcaactctat aattctcggc aagccttact atatttgtat atgttatcca
   126421 gtcatggcat gtcccgtgca taccgtgcag aagatacaat acctttaagt cttcgggctt
   126481 tgccaaagac ggcagtataa cattacattc atcctgataa taaagaagat tggaactgaa
   126541 tttgcaattg attaccgaag cacccatttc acacctcaca caatatattt gagtatatta
   126601 aaacgtccca agcaggaacg tcttatacta cagattatca aatacttcct taaaagcttt
   126661 aaccgaataa tcgatatctt cgtaagacaa cgcgtcggaa acctgtattc ttatccttgc
   126721 ggtaccctcc gggaccacag ggaaaccgaa tccggtaaca taaaccttct tttccagcat
   126781 agctttagct attctgatgg cctttgcggt atctcccaca attataggca ctattgcact
   126841 ttctccttcc aaaggctcga atccttcttc atgcagccgc tgacgcagat attttacctt
   126901 cttatgtaac gactctataa tgctgttatc tctcttaagc aactgtattg cttttaacga
   126961 cattgccgca acaaccggcg gaaggctgtt tgtgaatagc gatgttctgg aggcctggat
   127021 acacatatcg attacttctt ttttagcggc cacaaaacct ccggccgaac ctccgagcgc
   127081 tttaccgtat gttccggtta taatatctac cttgcccaaa agattatggt attctatagc
   127141 tcctcttccc gttgcaccta ctacacccat tccgtgggaa tcgtccacca ataagattgc
   127201 tttgtatttc ttgcacagtt tatatatctc atccagtttg gcaatatcgc cttccatgga
   127261 aaatacgccg tcggttataa ccagccttgt gttgaacctt tcgccttctt tcagaagccg
   127321 ctccagatct tccatgtccg aatgcttgta tacaaatctt tccactttcc tgctggttaa
   127381 acgacatccg tctattatac tggcatgatt caacgcgtcg gatatgatac agtctccttc
   127441 aaaaacaata gcgggaatta caccggtatt ggcattccag cacgacatat atgttaaaca
   127501 gctctcggta tgcataaact ccgccgtttc cttctcaagt tgcctgtgta tatcataagt
   127561 accgcagata aatctgacgg aagcggcacc tgcaccgtat ttatccaatg cttcatgtcc
   127621 tgcctgtatt acttccttgt tattgcaaag ccctatatag ttgttggaac acaatacgat
   127681 aacctcgtca tatttttcaa tatccgattt attactcata ggtgttgtaa gatactgata
   127741 ctctttgtaa gtacctccgt ctttcattgc ctgaagctta tttcttaaat tcgaataaaa
   127801 atccatgata cacatctcct tgtcatactt taagtcatat atttattata cactcttttc
   127861 atgtaacgtg aactaaaaca atgaatatat gtctatttga taaccttcca ccaaccgctt
   127921 tttttagaac cttgacgaat tacatatcct ttttctttta gtttggataa gttgcgctcc
   127981 accgtcttag gtgttttttc tatcttctta gcaataacac cgtatgtagc cctcggttct
   128041 ctcaatatta tttccataat atctttttca gttttattta tatcgacatt tgtagcgaca
   128101 tttattccga cattattctg aataacttca tttaaaacac ttctttcaaa gggaattgta
   128161 accataatat gattatcggt aatatcgaag acttcctttc cgtatttcct gattattgtc
   128221 gggacaccgt gccctgtatg ctccacaata tccaaatcgg aaaatatttt catcaacctt
   128281 aaatttctcg gtttgcttat tccgcggaaa aattcttctt tcgttaaccc gtgaggtaat
   128341 ccgccatggg aaaaaacttc caatctgtcg gtataaaaag atacttgcgg ttcgctgatc
   128401 aaccagtcgt tgtgaataat agcattgata actgcttctt ttacacagtc ataatcataa
   128461 agataggtat cttttctcgg tcttattgtt gtatcggtag tgcaaatatt ttccgaagca
   128521 atacggttca taagctgttc atacgcaaat atcaatgaac gctgtccgta atcagttcgc
   128581 tgtgaaatag atgctttatc caatcctctg aatttcacat atattaagga atacctatta
   128641 ttgtctgaca aaagctcacc cataatattg tattttcctg acggtgctat tagatacagg
   128701 tttgtctcaa agttatcatc gttgagccta tatcctttat caaggtaata ctgcttgagt
   128761 gaacgaaaag aaagtaaagg caggttggta accgcttccg ttattaaatc attattcaga
   128821 aacttcaatt tgtaacgatt tgcaatctgt tcttcagtca tttcacgaca ggtagagccg
   128881 atacgaacgg gacaacctat ggaggaaaaa ccgtattttt tgatgcagta tatgggcttt
   128941 gttcccttca aaaccgaaac ggaaacaagc acctttcctt cttccataag aatttcggtt
   129001 ttcacgctat ctgtcggaga cggctcaatt tgagcggtaa taatatctgc aatctgtttt
   129061 aaggtcttat caatctcatt aacacccact actgttccgt catcagttac accgataagt
   129121 attctgccgc catcggtatt caaaaacgca acaaccgcac ggataaattt atcgttcaat
   129181 ttctctttca attcaagccg gttggattca taatatttca taagatatca cctcataatg
   129241 attatatatc tatatgcgac attatgtcaa tatataccga catttatagc gacattacta
   129301 aagctctttt ataattactg ttcataggta tctcatattc tgcttcattg tttttgaact
   129361 aataatagaa atcgtacaaa ttgtacgatt tctaattata ctaaatcctt tattacaaca
   129421 agaaatcatt aattaacttt tctccaagca attgtattct ctattctctc ataagtacct
   129481 gcaccgcctg ctgtgtatgc atctctgaaa tagttacaat tgttaaaagc attatccgca
   129541 atatgcacac ccgagccaat ctccacgaca tttaatatct tggaaaagga aaatgcattt
   129601 ttctcaattc ttacaacact tgaaggaata gttaaagaag tgccattttg attgtgggca
   129661 aaagcacttt ctccgataac ttgaacagat ataccattta tagtagaagg aattgtaatc
   129721 ttatccgaag tgcctctgta ttgaactatt tcaccggttt ctgcattaaa tccaaagtca
   129781 ccatcaaata taggttcata tactgtaaca gtataaaatg cttcaaaact accatcaact
   129841 gtttttgctg ttataattga tgttccaact gattcacctc ttatatgagc atcaggattt
   129901 aatgacacaa cagccttatt actggttgtc caaataatac cccttattgt tgcatttgat
   129961 ggtagtatgg tagctggatt gctgtaaaac cagtatttcc cgccaacagg tatccgcatt
   130021 tcatcttttt caagagatat ccctgttaca cttgttactt gttctatttt ataccacgga
   130081 agggttttct ctctcgtgta tttaccggca ccacccgctg tatatgcatc tcggaaataa
   130141 ttagcattgt acatagcatt acttccaatc tggacatctg aaccgatttc tattatgtgt
   130201 agattattgc agaaactaaa tgcatttttc tcaattcttt ctacacttga aggaatagtt
   130261 actgaaataa gacaattatt attgtaaaaa gcattttctc cgataactcg tacaggaact
   130321 tcaccgatag tttcagggat ttctactgta gtatgagctc ctttaaaacc agttattgta
   130381 ccggtttccg cattgaatat gaaatcctct tcggtgacag ttttatagta aaccgtaaca
   130441 gaacatgatg cttcaaaatc tccatctgct gttcttgcag ttataaccgc tgttcctgca
   130501 aagttgcttt ttattgacaa cccatgtatt gacacaacag catcgttact gcttgtatat
   130561 atgatacctt tattagttgc atctgaaggg tggatagtag caagttggtc atagaactct
   130621 ccagctgtaa tcagcatatc attgttttca aaagacacac cagttacgct gattacttga
   130681 tcgattttat tccatgtatc attatcttgt cttgcataca tgcccgcacc atcaactgtg
   130741 tatgcatcta ggaagtgatt gtcatatcca atagcagagc ttgctatctg aacgcccgaa
   130801 ccaatttgta caacatatag gttatcacaa ccactaaacg caaattcatc aatcctttga
   130861 acactcggat gaatagtaac tgacgaaatg tgattattat tatagaaagc tttttctcct
   130921 ataacttgaa cagttatttc accgatagtt gaaggaatag ttactttttc ctgtgtgcca
   130981 gtatatttag ttattgtgcc tgtttcagga tcgaaaataa aatctccttc aacaactagt
   131041 tcatatactg taacagtgca gtatgcttca aaacctccat ctgttgttct tgctgttata
   131101 actgttgttc ctgcagagtt gactttcatg tttccagttt ccgatattga agcaacagct
   131161 tcattactgc ttgaataaat aatgcctcta atagttgcat caagaggttg gatagtcgct
   131221 ttaactctat aatactcatt ttctccaaaa ggtatgtgaa tttcattttt atcaaagaat
   131281 attcctgtta cactgattgt ttgattgatt ttaacccatg taccatgatc ttgccttgca
   131341 tatatccctg caccgcccgc tgtgtatgca tctcggaagc tgttgcccca attaacagca
   131401 taatctaaaa tctggacacc cgaaccaatc tctatgttgc agagattatt acagtcttta
   131461 aacgcatatt catcaaccct taaaacactt gggctaatag taaccgatgc aatgtgatta
   131521 ttcctataaa aagcttcttt tcctatagtt tgaacaggta ctccgccaat agttgaagga
   131581 ataattacgt tttcctcagc acctgtataa tgaactattg taccggtttc ctcatcaaag
   131641 gcaaaatctc cttcaagtac aagattatat actgtaacgg tacaagatgc ttcaaaaccc
   131701 ccgtctgctg ttcttgctga tataactgct gtacctgcag agttcccttt tataggacct
   131761 tcttctaatg acgcaacagc attattacta cttgtccatg ttataccctt aacagatgca
   131821 ttctcaggtt ggatagaaac agtaaggtca gagtattttt ctccaacatg tatccttatt
   131881 gattctttat taaaagatac tcctgttacg ctgattacct gattgatttt attccatatt
   131941 ggaccacttc tttcataaat accggcaccg gctggcacat atatatctcg gaaataattc
   132001 tcgtagcaaa aagcatcgtc ttcaatctca acacctgaac caatctccac gacatacagg
   132061 ttattacaag aaagaaatgc accatactct attttcacaa cacttgaagg aatagtaact
   132121 ccaataatat agctattttg ggaaaaagca ttttctccga taacctgaac aggtattcca
   132181 ccaataactc caggaatgat cactttatct tgagtacctt tatattgaac tattgtaccg
   132241 gtttccgcat caaatacgaa atcatcttca attataagct catatactgt aacagtacat
   132301 gctgcttcat aattcccatc tactgttttt gcagttataa ctgctgttcc tgtagaaata
   132361 cctttaatgc gaccatcact atttactctc acaacattct cattactact tgtccatgtt
   132421 acacccttaa tggttgcatt taaaggctgg atagtggcag gaacactaat ccagctctct
   132481 ccttcagcag gtattcgcat ttcattcttt tcaataatta ttcccgttac acttattaat
   132541 tgatcgaatt tattccatgt ccaaccttct cttgcataca tgccggcacc acctgctata
   132601 tatgcctcac ggaaagaatt attgttattt atagcatcat ttgcaatctg gacacctgaa
   132661 ccaatttgca ttacatatag attacagaaa cgaaatgcac catgctctat cctctcaaca
   132721 cttgaaggga tagttactga agcaatattc ctattgttag aaaatgcatt ttccccgata
   132781 accttaacag ttatttctcc aatagtttca ggaataacaa ctttatcctg agttcctgta
   132841 tacagaacaa ttgtgcctgt ttccggatca atcccgaaat ctccctcaat tacgggttca
   132901 tataatttaa cggtacatgc cgcctcaaaa ccgccgtcta ctgttcttgc ggtcataacc
   132961 actgttcctt cggaaagacc tttcatgcga ccatctccac ttaaaaccac aacagactca
   133021 tcactacttg tccatgttat actcttaatg gttgcatttg agggttgaac ggtagcaggt
   133081 atactaatcc agttatctct tacaacaggt attcgcattg catctttctc gatagttatt
   133141 cccgttacac ttattaattg atcaatttta ttccatatcc aaccatctct tgcatacata
   133201 ccggcaccac ctgctatata tgcatggtgt agagaatcac tgctatataa agcatcacct
   133261 gcaatatgga cacctgaacc aatctctaca acatataagt tattgcagtt aaaaaatgca
   133321 aatttctcaa ttcttacatt tgaaggaatg gttaccgaac taatataatt attacgataa
   133381 aacgcatatt caccgataac ctgaacaggt attccaccaa tagtttcagg aataattact
   133441 ttatcttcag tacctctata tcgaactatt gtacctgttt ccgcattaat ctcaaaatct
   133501 tcttctttta caggttcata tactgttact gtgcatgtcg cttcaaaacc gcaatctgct
   133561 gtttttgccg taataactgc tgttcctgca gaaacacctt gtatgcgacc atcaccattt
   133621 actctcacaa tagcctcatt actgcttgtc catgttatac ccttaatggt tgcatttgtc
   133681 ggttgaacgg tagcaggtat actaatccag ttatctcctc caattggaat acgcatttcg
   133741 ctgttttcaa tagttatacc cgttacattt ataaattgat taattttatt ccatgtccaa
   133801 ccatctcttg catacatgcc tgcaccgcct gccaaatatg cttctcggaa agaattattg
   133861 ttatttatag catcatctgc aatttggaca cttgaaccaa tttcaacaac atttaggtta
   133921 ttacagtatc gaaatgcacc atactctatt ctctcaacac ttgaagggat agttactgaa
   133981 gtaatattcc tgttgttaga aaaaacattt cccccgataa ctttaacaat tactcctccg
   134041 atagtttctg gaatagataa tttattttga gtgcctgtat actgaacaat tgtgcctgtt
   134101 tccggatcaa tcccgaaatc tacctcaatt acaggttcat atactgttac tgtgcatatc
   134161 gtttcaaaac cgccatccgc tgtttttgcc gtaataactg ccgttcccgt agaagcacct
   134221 tgtatgcgac catcaccatt tactctcaca atagcctcat tactgcttgt ccatgttata
   134281 cccttaatgg ttgcatttgt cggttgaacg gtagcaggta tactaatcca gttatctcct
   134341 ccaattggaa tacgcatttc gctgttttca atagttattc ccgttacatc gattacctga
   134401 ttaataatat tccaccttcc gttatcttgc cttgcatatg tacttgttcc gcctgctgtg
   134461 tatgcttctc ggaaataatt attccatcct atagcatcat ttgcaatctg gacacctgaa
   134521 ccaatctcta tgacatataa attattgcac catctaaaag cattttcttc aatttttaca
   134581 cttgaaggaa tggttaacga aacaatattc ttgttgtagg aaaaaacatt tcccccgata
   134641 acctgaacag ttactcctcc gatagtttca ggaatagata atttgttttg agtacctgta
   134701 tactgaacta tggtgcctgt ttccggttca atcccaaaat ctccctcaat tacaggttca
   134761 tataccgtca cggtgcatgt tgcttcaaaa ccgccatctg cagtttttgc agttatgact
   134821 gccgttcctg cagaattggc tcttatgtaa ccataatcat tcatcattga cacaacggaa
   134881 gcattactac ttgtccaaat aatacccttt attgttgcat ctgaaggttg aatagtagca
   134941 ttaattctat taggccggta ctctcctcca acagatatgc gcatttcatt attatcaaga
   135001 gatatgcctg ttacactgat tagttgatta actctattcc aactaccatc gtattgtctt
   135061 acatatttac ctgtaccacc tgctgtgtat gcctcacgga aagagttgct gttattaaca
   135121 gcattgtttc caatctgcac acccgtacca atgtctatga catataggtt atcgcagtat
   135181 tgaaatgcac catactctat tctctcaaca cttgaaggga tagttactga agtaatattc
   135241 ctgttgttag aaaaagcatt ctccccgata acttgaacag cttctccacc aatagttgca
   135301 ggaatattca ccatatcctg aacgcctgta tattgaatta tagtacctgt ttccgaatca
   135361 aatctaatat ctccctcaat tacaggttca tataccttta ctgtacatgt cgcttcaaaa
   135421 tcgccatcca gtgttctcgc cattacgact gccattcctg cagaaatacc tttcactcgc
   135481 ccatcaccac taaatgaaac aacattatca ttgttgctca tcaaaataat acccttaatg
   135541 gttgcatttg acggctggac tgtggcaggg aaactacgcc actctccaac aggtatacgt
   135601 atttcatcat aatcaagaga taatcccgta acatctatta aattatcgat tttattccat
   135661 ctccatccat ctcttgcata tgtacctgct ccgcccgttg catatgcatc gcagagagaa
   135721 tcacccccta ttgcttcatc ttcaagctgg acattcgagc caaattttat ggcatatagg
   135781 ttaacacaat taaaagcatt tttttcaact cttacgtccg aaggaatact aacataagta
   135841 atatacatgt cagaaaaagc accttctcct atagcctgaa caggtattcc actaatgatt
   135901 tcagggatag ttaatctgct ctgtcttcct gtgtgcagaa ctattgtgcc cgtttccggt
   135961 tcaatcccaa aatctccttc aaatacaggt tcatatactg ttactgtaca tgtcgcttca
   136021 aaaccgcctt ctactgtctt tgcagttata attgccgttc ctgcggaatt gcctctcatg
   136081 tgcctatcac cgtttactga tacaacagct tcattatcgc ttgtaaaaac aacaccctta
   136141 atggacgcat ttgatggttg tataattgta ttaggaatgc tgtaccatcc tccagcaggt
   136201 attcgcattt cgtctctttc aagagatatt cctgttacgt ttattagatg atcaattttt
   136261 ctccatcccc aataatcatc tcttgcatat gttccggcac ctcctgttgt gtatgtttca
   136321 aggaaataat aatggtagta tataaaagca tcatctgcaa cctgcacact cgaaccgata
   136381 tctacaacat tcaggttcca acagtacaga aaagcgccat cctctattct ctcaacgctt
   136441 gaaggaatag ttaccgaagc aatatgaaga tttccgcaaa aagcttcttg tccgatagct
   136501 tgtacaggta ttccactaat agtttcagga ataattactt tatcttgagt acctgtatag
   136561 tgaactatcg taccggtttc cgcttcaaac cagaaatccc cttcaactat aggctcgtat
   136621 actgtaaccg tgaaagatgc ttcataattg ccatcaactg tacttgcagt tataactgct
   136681 gttcctgcgg aaatgccttt catgcgacca tcaccattta ctaatacaac tgcctcatca
   136741 ctactactcc atcttatata tccaatagtt gcatcaaaag gctgtattgt agcagtaact
   136801 ctataagtcc agttcattcc tccagcaggt attcgtattt cgcttttagc aagagatatt
   136861 cccgttacat tggttatttc ttgggtttta cgccatatat tatcagctct gtattgtttt
   136921 acatatgtgc ccgctccacc ggatgtgtat gcattgcgga aatgatcttg gttaaaaaaa
   136981 gcattgtctg caatttgaac accagagcca atctctacaa gacataaata ataaccgcca
   137041 aatgcattgc taccaatatt aactacggta tctggtatta ttattttcca taatcccgac
   137101 ccataaaacg catattctcc tatattcaca atgctacttg gaatctctat agaatttaga
   137161 ctgaaacaac aggaaaacat agcatcacta atatacgtta attgtgaatc ttcttggaaa
   137221 gtaacattag taagacctgt agagcaggaa aataaatcat tccctatact ggttacactg
   137281 ttaggaatta caaacgaata gagttttggg caaccggaaa atgcacctat gccaatgtga
   137341 gacagatttg aagactcact gaatgttaca cttgtcaagt ttgcacaatt tgcaaatgca
   137401 taatcgccaa tacttacgat actgcttggt atagatatat taaccaagtt ggaacactcg
   137461 taaaaagcat aactctctat aaccgggctt tcaggattca tgccccgcaa ggaacacata
   137521 atacttgctc gcctaccttg cggagaggtt tgaaaatcta tatccgtaag attaacactt
   137581 gccataaatg ctccttctcc aatagttctt gtattaagcg gaatggaaaa tgctgcatcc
   137641 ggtcttcctg tcggatatgc aattaaagtc tttccatctt tgctgtataa aacaccgtct
   137701 atgcttgaga aagagagact tccttcttca acataaatgt ttagaagatt gtatgcatat
   137761 ccgaaagctc ccctacctat ctgtgttata ttcctgggaa ccgtataaga agtctcggtt
   137821 ctgcctccgg gataaacaac aagagatgta ttgtttttat tgtaaagtat tccgtcaacg
   137881 cttctatagc ttgcattatt ttcacttgtg agcaattctt tcagattcct gcagtaccag
   137941 aatgcacctt ctccaatgct tgttaccgaa tcagaaatag tgaatgatgc agcagttatt
   138001 cctgcaggat atgtgagtat agtacttccg tctttagagt acagaacacc gtctatgctt
   138061 gaataatcag catttgtttc actaactgct atttcaagta atgatctgca atctccgaat
   138121 atactctttc ctatttccct tactttttta ggaatatcaa aagaagcaag attaatacat
   138181 ccgaagaagg cataatcacc tattttctcc aagaatgaac caacttgatg atttactgaa
   138241 actaagttta cacaattctt aaatgcatta gcttgaatct cggttactgt gttgggtatt
   138301 gttacactaa caattgtatc attatccgca aatacagatt cagcgatttt aaaaacctca
   138361 acctcgttaa ttattgatgg tattgttaca ttaccgccat ttcctttgta acccgttatt
   138421 aatttattat atgcatcaaa ttcaaaatct ttttcaaccg agttagtata aacaacaatg
   138481 gtacattgag caattttgtt tccgtcaacc gtagttgctg ttattgtagt tttgcctaca
   138541 ctatgtcctg taactaatcc ttcactatct actgaagcaa tagagtcatc atcacttctc
   138601 catgtaaccg ctttattatc cgcattcgct ggtaaagcag tagctgtaag ttgttcagtt
   138661 gctccgggta aaattgccgt taccttttta ttaagactaa ctccggcaat cggtataagt
   138721 tccgttaccc atttggcgta tactgttaaa ttactggtaa caactgtgct ttcagtaaaa
   138781 gctatgttga acacattgtt atctacaaac catcctgcaa aatgcaatcc ttcttttgtc
   138841 ggattagcag gcaatgttac ggttgaatta tgctcaatat tccggattga atctactaaa
   138901 gaaccgctgt tactgtcaaa tgataatgta tatttattaa tctcataaag agcggttact
   138961 attaaatctc gtgtaacatt attgaataat atattccagt ttataaatac atatccctct
   139021 cttccgggta ctgtcggagc taccgcatct ttgcctttct caacagtttg tgtatccagt
   139081 acagtatcat tccaatcttt aaatgttacg gtatatgtaa tctgtttaac cggttctgaa
   139141 gtgctattgc tgacagttcc ggtgtatgag cctgatcctg ttgctataac tttgacatat
   139201 ttgttagcat catcatctgt cagagtatat gttcttgccg ttgctccttg aatatttttg
   139261 tattcgccgt cagatgattc cgctctctgc catcggtaag ataccgttgc cgaggacgga
   139321 tttactgtcg ctgttaacat ctgtccgaca gttgttgttc ccgtaatatt tactccgctt
   139381 accggtgtat atgtaggtgt aaccggaggt gtatatggag gtgtatatgg aggaatgaaa
   139441 ggtgtctgcc ctgtcgtatc cgttcccgta ctgttatttg tcgccgtgct acctccgccg
   139501 attgttttac cgcctgctcc ttttacatcc tttgctttac tgtcaacctg tattttcgta
   139561 ttgggggttt ctattttgga attacttcca tcttcttgaa ctttagcttc tttaaccgtt
   139621 ccttttcccg atacatttgc attttgagcg gctatttcaa ctttattaat ctctgaatct
   139681 ttatttacta ttaagttaca gttttcacct gttattgtcg tgctttctat ttttgcttct
   139741 cttgcaacta ccgttatatc attcgctact acatcaaggg tttgtacact tcccgtaatt
   139801 attacatcat ccgaaccgtc atctatgtaa ataacttcaa cattagattc gtcatcaact
   139861 tcaatacgca cttctccgtc cgctcttgat acaactacct ttgcaatagt tgaggaacct
   139921 ctgatgatta tggagtttac gcctccgcct cttaccacaa gtcttccttc aacatttaca
   139981 ctgtctaagg taacttcacc gtctccgact ccgtcggcta tgataagatc acccttaatt
   140041 gtcaggtttt ttaaggttac tccttctcca cgtataagaa cattgcctgt ttctactact
   140101 ttagtaacta ctcccggggt atatatattg ccttaccagt ctgttcatta ttgttgcgaa
   140161 ctccgctctc gtaatacttg ccttgggctt gagagttccg tcaggatatc ccgcaagata
   140221 tcccgctgca accatagcgt tcatttcact gcgcatactt ttagatattc ttgatttatc
   140281 tttaaagaca tccaatgccg tataatcatc acctaccgtt tttaacttaa aagcttttgc
   140341 taaggtggta aatacttcct ctctggtaac ggtccttgtt ggttgcattc ttcctttcaa
   140401 atcggtaata cccatctgaa cggctttacc gatactttct tcataccaaa ctcccttcgg
   140461 tatgtccact accgaagaca ggtctgctat aacttctgca ccgaaagctc ttgttactat
   140521 tgtaagcatt tcgcttcgtt tgagtacgcc gttaggtttt atgagctgct taccttcctc
   140581 aatgtaaccg ttaagcaaac cgttatttac cgcattgact aatgcttccg ttgcccaatt
   140641 gttcggcata tcggcaaaaa ccaagtttcc cgccttatct ctttcaagag ccgctgaagg
   140701 cactgaagta acaatcataa gtaatactaa aactatcgct aataacttga atctgtaaga
   140761 cctcatttta tacccccgag agaaatgtat aagatattta tatgatattg ttatcgtacc
   140821 atctccccac attttgtcaa tattttataa gaaaaaattg cctttttgct tgtattttta
   140881 tcaatttctc ataatttctc ataattttat cataagtata taacaactaa taattgtcta
   140941 ttgacagcgc aattgttttc aagtagcttg attgtattgg gaggcagatt atgagtaatg
   141001 aatttttcca ggagcaacgc gaacaatcaa ttatcaaatc aagaatagta atgaaatatt
   141061 tttcagcctg ggcaaaaatt atccttgcag aattgaagaa atatcctcat aacgatccaa
   141121 ggatggctta cgtggacttg ttcgcaggac cgggcatata tgaagaccaa aataaatcta
   141181 ctccgatatt ggttttagaa aagatactat ctgattctga tttgtgcgac cgggtaatca
   141241 ctttatttaa tgataaagat aaagcaaaca ttagtagttt ggaagcagta attgcacaag
   141301 ttcccggtat tgaaaagtta aagtatgcgc cgtgtttcta taatcatgaa gtgggtaatg
   141361 aaatagcaga aatgtttagt catcgaaaga ttgtcccaac attattcttt gttgatcctt
   141421 ggggttataa agggttatcg cttgatttaa tatcatctat tatcaagaat tggggttgtg
   141481 attgtatctt cttttttaat tacaatcgtg taaatatggg ggttcctaat gaagcagtaa
   141541 gacagcatat gatttcacta tttggaaagg aacgatttaa tttattactg gcaaacttgc
   141601 atgatgtttt aacttccgat gagcgggaaa tgattattgt tcaggggtta tgtgatgctc
   141661 taagagaaaa cggtagtaag tttgtcttac cttttcgctt taaaaatgat aatggaacaa
   141721 gaacaagtca tcatcttatc tttcttagca aagacttccg tgcatatgat attatgaaag
   141781 agataatggc aggagaaagc tctgactcaa aaaacggtgt agcaaatttt gagtataacc
   141841 cacgcgataa gttttacaag caaggtacat tattcgattt gctttccagt ccactagacg
   141901 acttgaaaga cggactaatg gagcaatata acaatcaaac tattgatttt ttagagcttt
   141961 acagagaaca cagcgttaat cgcccattta tcaaaaaaaa ttacaaggca gttttacgca
   142021 agatgtatga tgaaggctta attagtgccg taaatcgtaa aaccgataaa cccccgcaaa
   142081 aaggcacatt ttcagatgaa atgcgtataa cttttaggag caacaaatga gaactactaa
   142141 aatagaatgg acagacagga cttggaatcc ggtaacaggt tgcacaaaat tttcagcagg
   142201 ttgtgctcac tgctatgctg aaacaatggc aaacaggctt cgagctatgg gtattaaaaa
   142261 gtatgctaac gggtttttac cgacattgca tgaggactca ctatatgagc ctattaagtg
   142321 gaaaaaaccc cataccatat ttgtttgttc aatggctgac ctttttcacg aaaaagtaac
   142381 atttgatttt attgacagag ttatggctac aattagacaa acaccgcatc atcgctacca
   142441 aattcttaca aagcgtgcag aacgcatggc tgaatatttt tcacaaactg atatccccaa
   142501 aaatgtttgg cttggagtaa cagtagagtc atcatcggtc aagcatcgca ttgaattact
   142561 tcgtccttta caagctactg ttagatttct atcgtgtgag cctctacttg aagatatagg
   142621 tgaagtgaac cttacagaca ttgattggat tattgtcggc ggagaaagcg gcgtcaaagc
   142681 acggcctatg aaaccggaat gggttcgttc aatattacaa caagcgaacc aacaaggtgt
   142741 ggcattcttt tttaagcagt gggggacttg gggttctgac ggagtaaaac gtaataaaaa
   142801 agccaacggt aagacatttg acggaaagat aattcagatg atgccgcatt ttaaagaatg
   142861 cattaatctt gagtaatcta aaccgaagct caaaaagtct aaggtttttg catcaactga
   142921 attccgatta cttaatttgc ttagctttta aaccaaatca atgtttaatc tgacttcata
   142981 aatagagata aaatcaatat ttacttttgt tatattataa aatttaatat tagcacgaaa
   143041 ggaaatacca tgagtgacga atatgttgtt aaaaaactta cacaaaaaaa taacggatgc
   143101 tttacaccaa aagaagctgc aaatcaagga atcaacaaat atgattttta caagtttgca
   143161 gaaaaaacaa ctggagaaag tcggttacgg catctatgca tcatctgatt cttggttaga
   143221 cccgatgtac cttcttcatc ttagattaaa acaggttatt ttctctcacg aaacagcctt
   143281 atttctgcat agcatgaccg acagagaacc gattcattat ctcgctaaag aaaaatctgc
   143341 tgatgtacaa cttctgatgc gctcttatat aatggagcga cttccggaac gcatatctat
   143401 ctcgaaatat atgaagaatt tcattttgaa aggcggtata ttaattgctt catcggtagg
   143461 cttggattta cgtagaacga tggatattga cacgacgatt aagggatttg attaatccgc
   143521 aacaataacc gtcccgtcaa ccgatgagaa atatctttcc tgcccgttgt tatacatcac
   143581 tcgccataca ttgacaaaag acgatgtgtt cttatcatat atatatcccg catctatttt
   143641 ctttatggtg atgaaagaac gaccctgacg ggtaaacccg ctaaccaata tattgcctac
   143701 ggaaaccata ggtctgttat attccgtctc cgctacagta tattcgggac atccgatata
   143761 tacacggtct attccgttct tacttatcgt catttctatc ttcttatcgt aaatacagta
   143821 ggaaccgtca agatatatat aatttacctt atataccccg acaccggctg tcagtatgga
   143881 atcgggcatg tatgattggc tgtctaccgg cagtttctta atgaaatcat ccgctatctt
   143941 tgcagcagtt tccagtgtaa gatcctcata tctttgctta accgcaggag tatatatgat
   144001 tacgtcttcc tgtgaatcgg atatggtacc gccctgcgct tccagataag acaaaagcgc
   144061 actgttatcc gaatcggatt ttacaattac ggcactcggt gctgcatatt ggggtatttc
   144121 acaatccaaa gtgatatccc tgtatgcaag tatctcggtt atatacttat gctcatcttt
   144181 tgtatccggt tcggaataga caagattgat aataaaggcg gccatgaata tattgactgc
   144241 aagcagaagc acgattatta tcctgagagc ttttttagaa tccatcagtc ctgaacctcc
   144301 ttcagcccga gtaccgttat ttcctctccc tcaattatcc atttaggcag cagcacggta
   144361 ctgtcatctt tcatgtatgc aatatacatg ccgtttgctt ttatggagcc taaagccata
   144421 tcattttccg aaagcatatt aaatgtacgc aagtcgtaca taactgtagg taatgaggca
   144481 tcggaaatat caaaaagatt tgcatcggca gccaccactc tgtccgccgt tgcggttatg
   144541 cttatacaat acttctcctt atccagcagg acgggataac cgttgtagcg gtatacaaaa
   144601 cggaactcat agcaatcttc ctctattccg acaaaagcca acacgggcgg atttttctcg
   144661 gttgccgata acgaagtaag atcggcaagc atacttaaag cattgttgaa agctaaagca
   144721 atttctcctc tttcctgtat ctgtgatgca gaagcatacc tgtaagtaac cgagccgtcg
   144781 gtatgaatag tgtagatatt gtactggtcg gaaaaagtca aatccctgtt ctgtgcaatt
   144841 attaccctat atatatcgga ataaacaccg aacaatttgg ttttaatctc cgatatatag
   144901 cgtgtgacct gcggtgacag gttatttaca tccggctcta tctgaaggtt gagcatacat
   144961 tcggtcagaa gctcatatcc ggccaccctt atacttccga ttgcaacact gtcatccatt
   145021 atcaccacag tggtatcggg ctgaatattt cctttgaaat tgtcgtatga aaccacctcg
   145081 ttaagatata catatgttat atcgcttttt tgctttatct cttcatatat gcttaaaaac
   145141 tcattcccct taagtaaacc gttcagtttt acccctatat accgataaac cttttccccg
   145201 ctatttatat acacatcccc ttcatcggta ctttccggta aaatcattat cttatcaata
   145261 ctcttaatct cattattggt gttgaccgag tcgcttatcc ggttgataaa ctctatgggg
   145321 caatcatatc cgaacttgac tatatatccg gattttccgg tcatttcgtt ccacttatcc
   145381 gcatcatata tctcatagac tttcagctcg gagattgtat caagcagagt aaccgtatct
   145441 ttccatatct gagtgtaata tgccgaagat tgtttggtta tcttatggac attgagacct
   145501 tctccggaag tcatggttat ttccgacggc tgtatgtatg tttccagaag atactcttcg
   145561 aaattcatga cgtcggtcac agtatttgac cccggaataa ccttcttggg aagcagtctt
   145621 ccggtagagt gataattaat aagcataaat gactggaaaa cggtcagcag gattaatatt
   145681 ataagcagag gataagcgat gctctttctc attgtgccgc ctccttctta cccaacggta
   145741 tcgaaaccgt aatggtcgta ccctgattga cttttgattc cacctttata tcaccgtcat
   145801 gcgctttaac tatctcgctt gctatggcaa gacctaagcc cgttccgccc attatgcggg
   145861 ttctggcttt atccaccctg taaaatcttt caaacagtct ttcctgatct tttttggaaa
   145921 tacctatgcc gttgtctttg accgcaatac acaatttatg cgactcttcg taagccttta
   145981 tctctattct gcccgactca tcggtatatt taattgcatt gaccaacagg tttatcaggc
   146041 actgaagcat tctgtcataa tcgaaatatg cgatgccttt aaaagcaata tcggaaatca
   146101 atgtatgatt cttttgtttt gcctccgctt ccactgcctg taacgcatcg gttataaggg
   146161 aggttacatt gtgatgggat atcttcaata tcattcttcc catatccatg gaggacaact
   146221 gcaaaagatc ctttaccaga ttgtccatcc tgtccgattc ggaatttatc acctctaaaa
   146281 agtgctgctc ggtagcggga tcttccacac cttccattaa agcttcggta aaactcttta
   146341 tcgaagtaag aggggtttta agttcatgcg acacattggc aacaaactct tttctcattc
   146401 ggtccaatct gtactgctcg gttatatttc tgaaaagcag tatgacacct tcggcattgc
   146461 ccagactgtc cttaaaagaa gctatactga acgcaagcat ctcttcatca acgggaagta
   146521 tgtcttcttt ataaaaatcg gcgggaatat ccgacacatc aatatccaga ttatattctt
   146581 tcacgaagct ctccagcgtg aactccatat ccggtctgtt gagcagtgta tatgcagccg
   146641 gattgttatg tatcatctga ccgtcaaggt tgaaagctat aactccgcct tcgatattgt
   146701 ccagtattat ctccatcttg ttcttttccg aagatatttc atccaacata ttcttaaggc
   146761 tgtaactcat gttatttata cttgtggaaa gctgtcctat ttcgtcatcc gcctttacat
   146821 ccaatgttct tccgaaatcc ccttcggcaa ttgcctttgt acgaacggtc atatctttaa
   146881 tgggcctgct tatggctctg gataaaagat aacccacaac aaatgccacc gctatggaaa
   146941 acagcaatgc cgtaagcaac gtttcgttaa actgcataac catagcctgc caatcggttt
   147001 tgtaatagcg gaaatatata accaagtttt ccagtgttat ggcataatca tagaatgttc
   147061 ttccccttat attctgtaac gctttgctgt ttaacggtgt gcccgccatg gaagacagga
   147121 aattatatga caaaaacagc tcatttataa ataaagagct gtcgtgcgat aaaaaaaccg
   147181 aatcgttact gtatatttca ttggtatttc tgtccactac ggtaaaactg cggttatccc
   147241 cgtatatgcc gaatgaagaa gcataaaggt ctctcatatc ggtgtatacc gtatatatgt
   147301 taagaggttc cccttgcgta taattgagca ttttcctgcc gttctctata ttgcctgtaa
   147361 actgcgtgta atgatactgc tccacgttat tattcatgag cacaccgaca ggtattatca
   147421 tacatacggc tatgatacag atgtagaaga ccattttggt ctgtaaggat atatgcttat
   147481 cagtcttttt tgaaataata gcccattcct cttttcgtta ctatgtactc gttggggtca
   147541 cccgaaagtt tttctcttaa cctgctgacc gtgacatcta ccgtcctcat ctctccgtaa
   147601 tactcataat cccatatctt gtccagtaag aactccctgc tgaatacttt ttccgggttt
   147661 ctcatgaaga agaccagcag ctcaaactca cgtgcggtaa gctccaaaac ctgaccgttc
   147721 tttaatactt cgcattttcc ttcatcaact tccaaatctc ttatcttgaa ggataataat
   147781 tcggaattat ccacatttgc ataactgact ctgcgtaagt tgcttttcac tctggctata
   147841 agctctcttg cggaaaaagg ctttgtcata tagtcgtccg ctcccatttc cagtcctata
   147901 accttatcca gctcttcgct ttttgccgac agcattatta taggaacggt ggtgctttca
   147961 cgaagctttc tgcataccac aaatccgtca tattccggca acataatatc caataaaata
   148021 agatcgggtt tatgcttcaa tgcctgaaat atggcttctt tgccgtcata agcgcatatg
   148081 acatcataac cttctttttc cagattatac tttaaaatat ccacaatatt cttttcgtcg
   148141 tctacaacca atattttatg tcccataata catatccttt tttaataata tataaaaatt
   148201 ccataatttt caatcaatat gttcccggca ttattgtata gaatcctctg aaagcgaagg
   148261 atccgtcggg agctatgtaa gatataatgg cttcggtctc ggtagtaacc gtccctctga
   148321 caactatctt caacctcttg gcaacgggat caaatgtaat ggaatccaca aaattgctgt
   148381 tacacaacca cctggtattg gaaggtaaat tggcttcaat atcatattgg tcatatatcc
   148441 gtaaaggttc ggtatagaat tctcttgagg atataacaaa tgactgattg gcatcatatt
   148501 caatcctgct gaccttggac tcggcggcca acaccactat atctgccgac ttgacttcct
   148561 tgctttcttt ccagaattta accgtgacaa tatcattctc ggtataatac gagtggaatt
   148621 taatagccca tgttgcggta tcaacataaa ctttgttccc ggtaacggtt accgatgata
   148681 ttacggtagg aagtcccgat tcatctgttt taagcattac cctatcattg gacaatgtat
   148741 atcctcttat ggatatatat ttggtatgag catcaatcct gttattagct cctccgtaaa
   148801 taacttccgg tacctctata tcgaatatct gtatattctc cgaatccaca ttggtaaggc
   148861 tgaaactgta acccgacctg tccattattc ttccgtcacg ggttatgtaa atattaaata
   148921 tatcggttcc cggaatatcg gttgtagtaa agatattacc ttcatatgca aaactgttac
   148981 ccgatacttt atctatatat acaccccatt ccaccgtatc ccatgaaggt ctggaactta
   149041 acgaattggg gttaccgtac tggtcaacca caacaacatc atccaaatcc aatacaacat
   149101 tctttactcc cttttcaaaa tgagtaaagg gcatttccat tcctgtaact atatacggta
   149161 catttccgtc atttacattt acggtaagta aaggctgaat accgccttta atactgtaat
   149221 tcacggtagc aacaccgcta cttaaagtat tgaaagacaa cactccgtta cttatctgta
   149281 tatttgccgt aggtactacc gcggtatccg atgatgtata tgacaccgca tcagccgctg
   149341 tcatttcata tatattccca tactggtcat agcccattac atttacttca attctctcgt
   149401 ttttaaatat ggtggaaggc aattgttccg cttttatact tgacaaaaac ggtgcctgtg
   149461 atacctgtat atccgccgat gtggatatgc cttcttcctt tacaataata tgtaatcttg
   149521 tatctccggc ttttcccgca ttaaaaagaa gattgccttc gctgtttacc cttatacttg
   149581 atacgcttac gcttaaggaa ttggaggaaa taagcatcaa ggagccgctg tctatatcgg
   149641 cttgccttac cttatacact tgcccgtact ggtcataagc cgacagctta ataacatgca
   149701 tggagctatc ttcaaatatc ctttcctttc ccgatgcttc cgtaacttca gttacactga
   149761 tacgtttaat agccggcacc gactgaacgg taagctgaac cgaatcgctg agcagcgtct
   149821 tattatccaa agcatatata tatatctgat cccctatatt cgttaaagcg gcatctataa
   149881 gtaccgaagt ttcggtctta ctgataacgg cttttcttgt ccccgatgtc ttattggttg
   149941 cggacacatt aacatttgcc aaagacatgg attctccgta ctgattcaac aacttcaacc
   150001 ctatatcctg cccgaaagtc ttatacaacc tgtcaccggt tattatcaaa gactgcgcct
   150061 tttccgcttc cacggttaaa ttctgcactt ttcccccgac aaccaccgta tatgtaccgg
   150121 ggctcaatgt agccgatgtg gttataacaa gcttctttct gttagcggaa aactcatatg
   150181 tattggctat tcctatcagg tcttttctta aaacaacgga tgtatcagca gacacgggca
   150241 taaagaactg caccgttata cttcttactc ccgtaaccga agcaccggaa atcataacct
   150301 ccgtactttc tacaacaaaa ccgtactttt ccgcccgctc cagactgata acatcggctg
   150361 ccacaagctc ggtaaccagt gtatatcctt ccttggtacg tatattcaaa gcttccacca
   150421 gtgctacagc gacatcggta ttggtaatac ccgtcttatt cttcaaggag cgtaatccta
   150481 ccgattcggc aaacaaaagt gtactattcc aggaaaaatc cacatcctgt ctgtatccca
   150541 gcacagtcaa caatacttta gcaagcatct gagcatttat actgccgttg ggattgaatg
   150601 taccgtcacc gaaaccggaa aaccctacag aaggattggc atacagatac gccagtatgg
   150661 gctgccagta aggagcagcc gaagaagcat cacgaaaagt agcggttccc tcacactcca
   150721 gagcttcatc ttccaacccc aataatctga gtataatcat ggcggcatta cttctggtcg
   150781 gaacggagga aagataagcc tcatccagac cgcttccggt tcctctaaga atatttaacc
   150841 cgtatgctat ttccaccgaa gtatatgcct ttggaaatac aacacaataa ctcaacagaa
   150901 caaccgctat taagatgata ccaagcgctc ttttcataac atgtacatta tatcagataa
   150961 atatatatga ataggaaatg taacaaaact gatacaatcc
//
LOCUS       DAOSOM010000002       138876 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_91_length_138876_cov_7.116344, whole genome shotgun sequence.
ACCESSION   DAOSOM010000002 DAOSOM010000000
VERSION     DAOSOM010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 138876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 138876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..138876
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91_length_138876_cov_7.116344"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(423..1100)
                     /locus_tag="PLF14_00595"
     CDS             complement(423..1100)
                     /locus_tag="PLF14_00595"
                     /inference="COORDINATES: protein motif:HMM:NF025643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4367 domain-containing protein"
                     /protein_id="HOT69830.1"
                     /translation="MTETKYTLRDAFIEAKRIKLSGIPKDEDIDWTPSPRFERKINRL
                     IKNSRKSYWPLINTPLKKVAVAAMVIIMLFGFTMTVKAAREAVVDYIITVYEKYTAFS
                     FDVRGLDVPKTIEQKYMVTALPEGYTLSSKDEMDFRRKFIWTNEKGEDIIFKQSVLHN
                     TSMTLDTEDAPYKYVKVGQYTAIYSTKRNMQTVIWDNGKYLFIIDCTTSVSYEDVIRM
                     AESVAEE"
     gene            complement(1093..1656)
                     /locus_tag="PLF14_00600"
     CDS             complement(1093..1656)
                     /locus_tag="PLF14_00600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT69831.1"
                     /translation="MLIFLSLIDDDEGKEKFGALYNLYRKKMFFVANEILHNECDAED
                     AVQETLLSLARNIKNVDVVHSKKAYYYVMTITENTALNLLKKKKGESRKIINLDFDDD
                     SWLFVSDSDIAEEFVSKEEYNRIVDIIQSLPDIYSDVLYLHYVENLTAREIASALSRK
                     QETVRKQIVRGKKMLIYYLRKEGMEND"
     gene            complement(1880..2746)
                     /locus_tag="PLF14_00605"
     CDS             complement(1880..2746)
                     /locus_tag="PLF14_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HOT69832.1"
                     /translation="MKAQERKKIISVKNVSFTYAKNTPFEKKALEDINLDFYGNEYTA
                     IIGATGSGKSTLIQHLNGLLKPDKGEVTVDGIDTKDKKAQGDLRRKVGLVFQYAQYQL
                     FEETVYKDIEFGLIKRNVPEEERKKRVPKAAKLAGLTEENLKVSPYDLSGGQKKRAAI
                     AGILVLKPEILVLDEPAAGLDPEGTRELFATIQNLHKSKECTVILVAHSMELVALYAE
                     RIIVLDQGKVVMDGTRDEVFGNEEKLLSLGLDIPEITKVMKKIKEKHPNINDRIYTVD
                     DAKEEILKNLAK"
     gene            complement(2746..3573)
                     /locus_tag="PLF14_00610"
     CDS             complement(2746..3573)
                     /locus_tag="PLF14_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HOT69833.1"
                     /translation="MIEVKNAHYSYKTQPETTKAVSGISLSIAKGRYIAILGRNGSGK
                     STLARMLNALIIPDSGDIIVLGMNTKEKENQLPIRKKVGMIFQDPDNQIVGTRVLEDV
                     AFGPENLGYERQKIIQRVDDALHQIGIYDLRERAVHTLSGGQKQRVAIAGVLAMRPAC
                     IILDESTSMLDPKGQAELLSVVDKLNKEEGISIINITHNMDEARFADEVYVLHQGTVT
                     LKGSPEEVFTDVDNMLKAGLDVPQATKLLYELKKEKINIEDLTIDRNRASDIIGRLL"
     gene            complement(3616..4122)
                     /gene="rplQ"
                     /locus_tag="PLF14_00615"
     CDS             complement(3616..4122)
                     /gene="rplQ"
                     /locus_tag="PLF14_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOT69834.1"
                     /translation="MPSHRKLDLPTDQRIAVLKNLVTGLIMTGKIETTEARGKEVKKI
                     ADSIIAKAVKECDNFTSKPQKVTRPKTHKGRRLTMTKESKYKVDYEVIERETVEEMVT
                     VDSPSRLHARRQIFKWVNKVDGVNVVNKLFDEIAPRLKDRQGGYVKIYKLGKRRGDAA
                     EMVLLEII"
     gene            complement(4134..5072)
                     /locus_tag="PLF14_00620"
     CDS             complement(4134..5072)
                     /locus_tag="PLF14_00620"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOT69835.1"
                     /translation="MTIEKPRIECVETTDVYGKFVVEPLERGYGTTLGNSLRRILLSS
                     LPGAAATSIKIDGVLHEFSTIPGVVEDVTEIILNVKGIRFRLDTDEVKTLSIRTEGTG
                     DVTAGDILKNAEVEVLNPDHYLFKMDECKSFYMEITVSKGRGYDSAEKNKKEGMPIGT
                     IPIDSIYTPVNRVVYKVEDTRVGQITDYDKLTMEIYTDKSMSPTKALSYAAKIMSDHI
                     SLFIDLSEEVKQTETVKEAEEINKDESLEKPIDDLDLSVRSYNCLKRAAINTVGDLIS
                     KTEEEMMKVRNLGKKSFEEIHNKLDELGYKFAKSDE"
     gene            complement(5124..5750)
                     /gene="rpsD"
                     /locus_tag="PLF14_00625"
     CDS             complement(5124..5750)
                     /gene="rpsD"
                     /locus_tag="PLF14_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOT69836.1"
                     /translation="MARYTDAVCKLCRREGTKLFLKGDRCYTDKCALVRRAYASGQHG
                     KRRSKQSEYGMQLREKQKARRFYGVLESQFRKYFEMASRQKGMTGENLLKILESRLDN
                     VVYRIGLANSRPEARQLVKHGHFTINGKTVDIPSYLVQVGEVIQLKESMKDSPKFKEL
                     IETSQTKIIPAHIEFDQSAVSAKIVDTCKREDVSLPVEERFIVEFYSK"
     gene            complement(5766..6164)
                     /gene="rpsK"
                     /locus_tag="PLF14_00630"
     CDS             complement(5766..6164)
                     /gene="rpsK"
                     /locus_tag="PLF14_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOT69837.1"
                     /translation="MAQKTTVKKARKKKEKKNVEHGQAHIRSSFNNTIVTMSDLQGNV
                     LSWASAGGLGFRGSKKNTPFAAQVAAETAAKAAMEHGLRTVEVYIKGPGAGRESAIRA
                     METAGLTVVMIKDVTPIPHNGCRPPKRRRV"
     gene            complement(6178..6549)
                     /gene="rpsM"
                     /locus_tag="PLF14_00635"
     CDS             complement(6178..6549)
                     /gene="rpsM"
                     /locus_tag="PLF14_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOT69838.1"
                     /translation="MARIAGVDLPREKRVEIGLTYIYGIGLTSSQKILEQAGVNPDTR
                     VKDLTESEIVKLRGLIEKEHVVEGDLRREVSFNIKRLVDIGSYRGRRHRMGLPVRGQR
                     TKTNARTRKGPVRASVSVRKK"
     gene            complement(6657..6770)
                     /gene="rpmJ"
                     /locus_tag="PLF14_00640"
     CDS             complement(6657..6770)
                     /gene="rpmJ"
                     /locus_tag="PLF14_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOT69839.1"
                     /translation="MKVKPSVKKRCEKCKIIKRKGRVMVICDNPRHKQRQG"
     gene            complement(6783..7001)
                     /gene="infA"
                     /locus_tag="PLF14_00645"
     CDS             complement(6783..7001)
                     /gene="infA"
                     /locus_tag="PLF14_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008900989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOT69840.1"
                     /translation="MLAKDVIETEGVVIEALPNTMFRVELQNKHVILAHISGKLRMHY
                     IRILPGDKVKIEMSPYDLTKGRIVWRGK"
     gene            complement(7024..7689)
                     /locus_tag="PLF14_00650"
     CDS             complement(7024..7689)
                     /locus_tag="PLF14_00650"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013429981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOT69841.1"
                     /translation="MIVIMLGAPASGKGTQSVIVAQRLKVPHISTGDIFRWNIANNTA
                     VGEEAKKYIDKGILVPDELTIRILKERIEKEDCERGFVLDGFPRTINQQQSLDRMLTE
                     CNKSINMAINLSVTDDEVIRRISGRRMCKCGKAYHIVTYPPKTPGICDLCNGELYTRK
                     DDTVEVANTRLEAYKLQTEPILAVYRERGIVVDIDGHKPQEQVAEDVWKEILKYSEKH
                     GWM"
     gene            complement(7697..8980)
                     /gene="secY"
                     /locus_tag="PLF14_00655"
     CDS             complement(7697..8980)
                     /gene="secY"
                     /locus_tag="PLF14_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOT69842.1"
                     /translation="MFQTIRNAWRIPELRKKMLFTIAMLLIYRVGCHLPVPGLIGAAF
                     EVLVEGNKLFGFMDMFSGGAFSNMSLFAMNIQPYINASIILNLLTVAIPALERLAKEG
                     EEGRKKITQYTRYLTIGLGLFQASMLFITLKNYDVLYDGGTVLSFFTVVFTFCAGTAI
                     VMWLGELITEKGIGNGISMIIFVGIVSRIPTAAAVLYANWQQGNLGATAIETILLVTG
                     LLVVMLGMVVGTVYVSGATRKIPVNYAKKVVGRKMYGGQSTHIPMQLNMSGVMPIIFA
                     SSITTLPSIIINFVAPNTESKFLIALQKTDSWGFLAVYAALIFFFSFFYTMIAFNPVE
                     VASNLRKNGGFIPGFRPGKPTSDYLAYVLNKITWFGGLFLALLATIPSILAKVVGIQI
                     GFGGSALLIVVGVALETVKQMESQMLMRHYKGFLD"
     gene            complement(8980..9423)
                     /gene="rplO"
                     /locus_tag="PLF14_00660"
     CDS             complement(8980..9423)
                     /gene="rplO"
                     /locus_tag="PLF14_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOT69843.1"
                     /translation="MKLNQLAPAEGSKKKATRKGRGHGSGSGKTCGRGANGQNSRSGG
                     GVRVGFEGGQMPLYRRIPKFGFNNYRFQKNYAEVNIETLNIFKNKTVVDEALLREAGI
                     VKGVCDGIVILGRGELTKNLTVKASRFTKSAAEKITAAGGKAEVI"
     gene            complement(9437..9637)
                     /gene="rpmD"
                     /locus_tag="PLF14_00665"
     CDS             complement(9437..9637)
                     /gene="rpmD"
                     /locus_tag="PLF14_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOT69844.1"
                     /translation="MAKKKAEQAKMLKITLVKSANNSIQKHKATVAALGLRKIGQTVE
                     QKDNEKIRGMIQKVRHLVSVEE"
     gene            complement(9647..10150)
                     /gene="rpsE"
                     /locus_tag="PLF14_00670"
     CDS             complement(9647..10150)
                     /gene="rpsE"
                     /locus_tag="PLF14_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOT69845.1"
                     /translation="MRNDFDRTPSEFKEKVVRIGRVAKVVKGGRNFRFSALVVVGDEN
                     GRVGAGMGKAAEIPESIRKGTDDAKKNLINVPLDGTTVPHEIIGQYGAARVLLKPAAP
                     GTGVIAGGTVRAVLDLAGIKDVRTKCLGSHNAVNLAKATIDGLSRMMTVEDVSKKRNK
                     SVEEILG"
     gene            complement(10169..10537)
                     /gene="rplR"
                     /locus_tag="PLF14_00675"
     CDS             complement(10169..10537)
                     /gene="rplR"
                     /locus_tag="PLF14_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOT69846.1"
                     /translation="MIKKTDKNAARKKKHDRLRNHFSGTAEVPRLNVYKSLNNIYAQV
                     IDDTKGNTIVSASTLDQSLKGKLKYGGNMEAAKEVGTLVAKKALEKGIKNVVFDRGGY
                     VYHGKIKALADAARAAGLEF"
     gene            complement(10543..11082)
                     /gene="rplF"
                     /locus_tag="PLF14_00680"
     CDS             complement(10543..11082)
                     /gene="rplF"
                     /locus_tag="PLF14_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOT69847.1"
                     /translation="MSRIGKLPITVPAGVIVDIAKDNTVTVKGPKGTLTQTFNKSMTI
                     TKDGDVINIERKSEEKKQKALHGLTRSLLNNMVHGVVNGYQKKLEINGVGYKAALDGQ
                     KLVLNLGYSQPLEVIPPEGITFEVPTPVSIIVRGYDKQAVGQIAAQIRAYRKPEPYLG
                     KGVKYSDEIIRRKEGKTGA"
     gene            complement(11156..11551)
                     /gene="rpsH"
                     /locus_tag="PLF14_00685"
     CDS             complement(11156..11551)
                     /gene="rpsH"
                     /locus_tag="PLF14_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOT69848.1"
                     /translation="MQISDVIADMLTRIRNAGTAKHERVDIPGSNLKRDIADILLEEG
                     YIENVEWIDDEKQGVIRITLKYQDKKNVITGIKRISKPGLRVYSSKDELPKVLNGLGI
                     AIISTSKGIMTDKKAREAGVGGEVLAFVW"
     gene            complement(11564..11749)
                     /locus_tag="PLF14_00690"
     CDS             complement(11564..11749)
                     /locus_tag="PLF14_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002836123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOT69849.1"
                     /translation="MAKKAMILKQRKPQKFSTREYNRCQICGRPRAYLRKYGVCRLCF
                     RELAYKGEIPGIRKASW"
     gene            complement(11763..12302)
                     /gene="rplE"
                     /locus_tag="PLF14_00695"
     CDS             complement(11763..12302)
                     /gene="rplE"
                     /locus_tag="PLF14_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009372736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOT69850.1"
                     /translation="MILQQLYKEKAIPALMERFKYTNVNQVPKLVKIVVSIGMGEAKD
                     NPKAMDAAAADLTAITGQKPVVTTAKKSVANFKVRQGMKIGLMVTLRGARMYDFANKL
                     INIALPRVRDFRGLNLTGFDGRGNYAMGLKEQLIFPEIIYDNIDKIRGMNLVFVTTAK
                     TDEEARWLLTELGMPFKKA"
     gene            complement(12313..12639)
                     /gene="rplX"
                     /locus_tag="PLF14_00700"
     CDS             complement(12313..12639)
                     /gene="rplX"
                     /locus_tag="PLF14_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOT69851.1"
                     /translation="MHVKKGDNVLILSGNDAGKSGKIKDVNKTKCTVIVENANMGKKH
                     VKPRKAGEAGGIVDFERPIHASNVMLICPKCKQASKTGMTFDKDGNKQRTCKKCGAVI
                     DKVGAK"
     gene            complement(12656..13024)
                     /gene="rplN"
                     /locus_tag="PLF14_00705"
     CDS             complement(12656..13024)
                     /gene="rplN"
                     /locus_tag="PLF14_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOT69852.1"
                     /translation="MIQAQTRLKAADNTGAKELFCFKVLGGTGRYYANIGDVIVCSVK
                     TASPGGLVKKGDIVRCVIVRSKKGIKRKDGSYIKFDENAAVIIKADKEPVGTRIFGPV
                     ARELRDKEYMKIVSLAPEVL"
     gene            complement(13029..13286)
                     /gene="rpsQ"
                     /locus_tag="PLF14_00710"
     CDS             complement(13029..13286)
                     /gene="rpsQ"
                     /locus_tag="PLF14_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOT69853.1"
                     /translation="MTTRNLRKTRVGNVVSDKMDKTVVVAVVDSVKHPLYKKIIKRTY
                     KLKAHDENNECAVGDRVRVMETRPLSKDKRWRVVSIVEKAR"
     gene            complement(13288..13485)
                     /gene="rpmC"
                     /locus_tag="PLF14_00715"
     CDS             complement(13288..13485)
                     /gene="rpmC"
                     /locus_tag="PLF14_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOT69854.1"
                     /translation="MKANDIRKKTNEELAVELNQLKSELFKLRFQYATNQLENPMKMK
                     SVKKDIARVKTIIREREIKEI"
     gene            complement(13475..13903)
                     /gene="rplP"
                     /locus_tag="PLF14_00720"
     CDS             complement(13475..13903)
                     /gene="rplP"
                     /locus_tag="PLF14_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOT69855.1"
                     /translation="MLIPKRVKYRRVHRGRMKGKALRGNTIDQGDYGLVAAEPGWIKS
                     NQIEAARIAMTRYIKRGGSVWVKIFPHKPVTKKPAETRMGSGKGAPEYWVAVVKPGRV
                     MFEIGGVAEADAKEALRLASGKMPIKCKIMARESGEANEG"
     gene            complement(13903..14562)
                     /gene="rpsC"
                     /locus_tag="PLF14_00725"
     CDS             complement(13903..14562)
                     /gene="rpsC"
                     /locus_tag="PLF14_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOT69856.1"
                     /translation="MGQKVNPHGLRVGIIKEWDTKWYANKKDYSKYLVEDNTIRKFIK
                     KKLYIAGISKIEIERAANKVKINILAAKPGLIIGKGGAGVEQIRADIEKLTTKTVLVN
                     ITEVSHIDMDAQLVAEGIAAQLEKRVSFRKAMKQAMGRTMKQGALGIKTMASGRLGGA
                     EIARSEHYHEGTIPLQTLRADIDYGFAEADTTYGKIGVKVWIYKGEVLPAKKDDEGGI
                     Q"
     gene            complement(14574..15011)
                     /gene="rplV"
                     /locus_tag="PLF14_00730"
     CDS             complement(14574..15011)
                     /gene="rplV"
                     /locus_tag="PLF14_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOT69857.1"
                     /translation="MEKTTENKEKTFTRKCKKAPRLTKKQRKELGVGKDQGRASINNV
                     SVPATKAKLVIDHIRGKDYEVALAIVKNTPNIITETLEKLLVSAHANAVNNNNLDESK
                     LYVAEIYADQGTTLKRIKALSRGSATRINKRTCHLTVVLKERV"
     gene            complement(15023..15304)
                     /gene="rpsS"
                     /locus_tag="PLF14_00735"
     CDS             complement(15023..15304)
                     /gene="rpsS"
                     /locus_tag="PLF14_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOT69858.1"
                     /translation="MSRSNKKAPYVFPRLLEKIEKMNETNDKRVLKTWSRSSTIYPQM
                     VGHTIAVHDGRKHLPVYITEDMVGHKLGEFVLTRTYRGHGKGDKATRVR"
     gene            complement(15317..16150)
                     /gene="rplB"
                     /locus_tag="PLF14_00740"
     CDS             complement(15317..16150)
                     /gene="rplB"
                     /locus_tag="PLF14_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOT69859.1"
                     /translation="MPIKKYNPTSPARRFMTVSSFKDITGKASKKSLLSPIKREGGRN
                     NHGRITVRHQGGGAARKYRIIDFKRDKDGVPARVAAIEYDPNRTSNIALLFYADGEKR
                     YIIAPVGLAVNDTVMSGPTADIKVGNALPLANIPVGTMIYGIELKPGKGAQMVRSAGN
                     TAQLMSKEGDFAQIRLPSGEVRMVSVNCKATIGQVGNVDHANIRIGKAGKKRHMGVRP
                     TVRGSAMNPVDHPHGGGEGKAPIGRPSPVTPWGKRTLGTKTRKKKKASDSMIVKRRHS
                     K"
     gene            complement(16168..16524)
                     /gene="rplW"
                     /locus_tag="PLF14_00745"
     CDS             complement(16168..16524)
                     /gene="rplW"
                     /locus_tag="PLF14_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOT69860.1"
                     /translation="MKYYEDIIIRPYITERTNEQAMLGRYTFMVDKKATKIEIKQAVE
                     KLFSVKVLKVNVMHYQGKEKRVGVHLGRTASFKKAVVTIDTDPKPETYLDKGGKIKTV
                     AKKYKTSIEEFGVTPH"
     gene            complement(16524..17147)
                     /gene="rplD"
                     /locus_tag="PLF14_00750"
     CDS             complement(16524..17147)
                     /gene="rplD"
                     /locus_tag="PLF14_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOT69861.1"
                     /translation="MLKADVYNIKGEVVDTMSLNEDVFGVEVSESAIHSAVVQYLANA
                     RQGTQSTKTRAEVRGGKTKPFRQKGTGRARQGSSVGPHQIGGGVAFAPKPRSYRFSLN
                     KKQKRLALKSALSQKFGSGSFIILDSLKMDEIKTKKFAEILNNIKVDTSCLVVLGEKD
                     DIVVKSARNLPNVATALTNTINVYDIIKYDKFVATKEAVEKIQEVYA"
     gene            complement(17161..17790)
                     /gene="rplC"
                     /locus_tag="PLF14_00755"
     CDS             complement(17161..17790)
                     /gene="rplC"
                     /locus_tag="PLF14_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOT69862.1"
                     /translation="MKNFMLGKKAGMTQLFLEDGQAVPVTVIEAGPLTVVQIKTKEHD
                     GYNAVKVAYGDVKESRVNKPEKGLYEKSSLTPKKFVREFRVDSTEGYKVGDVITVETL
                     KEGMLVDVIGTSKGKGYAGTTKRYGTAIGRASHGSKYHRGVGALAMSSTPSKVFKGRQ
                     MPGHMGAERVTVQNLEVMKVYADRNAVLVKGAIPGPKGGLIMIQDAKNA"
     gene            complement(17849..18160)
                     /gene="rpsJ"
                     /locus_tag="PLF14_00760"
     CDS             complement(17849..18160)
                     /gene="rpsJ"
                     /locus_tag="PLF14_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOT69863.1"
                     /translation="MASKQRIRIRLKAYDHELIDQSANKIVETAKRTGAKVSGPVPLP
                     TEKEIVTILRAPHKYKDAREQFEMRTHKRLIDILNPMPKTVDALMLLDLPAGVDIEIK
                     L"
     gene            18624..20870
                     /locus_tag="PLF14_00765"
     CDS             18624..20870
                     /locus_tag="PLF14_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT69864.1"
                     /translation="MISALVGSFVQTAGWTAKIEDLRNKTNTGTITIKATDSEEAKDY
                     TVNGKVVSGILITPKNATKDHPAPGVVFTHGLFNNREMQLQNGIEAARRGYVVLLIDR
                     ESHGHNDTTTYDNYGDSMLSAAKYLYNLTDPQGNRIVDGSRILVSGHSMGGSATNGAL
                     MLDGVSNKTAITVGDTKYTWEGQVDEALSKGYHMGIISAGIVQANNAQNASYGTNLKG
                     VSVLKASSDEFFFASTTKNGIYRPLNLNQNTELLFTQGINEKVTTNRSLDGKLYVKKG
                     DDYVAITSSDTYHKKTQYYVFTKNGNSPFYLMSSQAMNFVGKPLESATGEQYEVINGG
                     IYDFNKEGALIGQPKDNKYLSAQTGGAQQASDTVQIRAVYEAKENHPMNHFSRMSAAG
                     IIDFCYNVFGVAEGTTFKAPNNQTWWIKEVFAIFGIAGLFGLLVILVDLLLKTKAFAS
                     LAAAEGDIPEAPVLLTKPRKHVFYWLSGILTAWYGYYAWENIVRGKNWYANSFWRTLI
                     TDTTSEITGQTYTSWANAGTIAYWGICCATFALIITAVIWLINRVINMIVYKDDYLAH
                     EERPFLGFRIRSVGNIFKTLALAAILCFVFFGTIQLLWNVLVVDFRAWTFAFRIFKTD
                     RLLSYLRYVPFFFVFYMTNAALNINYRVKDLPEWATVLINVVFNVGWLIISVWYFNSY
                     FINVGAFTNSTLSMQNIWTYPIIPCVATATIVSRRLYVRTGNAWLAGLVNATIMALVC
                     CAGTSIGA"
     gene            complement(21410..21715)
                     /locus_tag="PLF14_00770"
     CDS             complement(21410..21715)
                     /locus_tag="PLF14_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOT69865.1"
                     /translation="MYEIRFTSAAEKYFKKVKEKKLLNEYREAINKISKNPYSGEIKK
                     GNLAGIYCYNVYYSSVNYEIAYRIFEQNEKFIVVILAGTRENFYQQLKRYIDNHQIL"
     gene            complement(21715..22086)
                     /locus_tag="PLF14_00775"
     CDS             complement(21715..22086)
                     /locus_tag="PLF14_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOT69866.1"
                     /translation="MISYLENNQRKRKIISVSKKRQITIPQKFFEALDLGNEVECVLQ
                     EDSILIRPISVNMADEYSDFILSELVEQGYGGQELIIKFKEKRKKIKSALENLLKEAD
                     DIAAGKKKGATMEEVFGQVDE"
     gene            complement(22632..22913)
                     /locus_tag="PLF14_00780"
     CDS             complement(22632..22913)
                     /locus_tag="PLF14_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009535049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HOT69867.1"
                     /translation="MANTLVQFRIDEAERAEAAQICMHLGIDLPTYFRMCVSRLVKEG
                     GIPFSMKLEDINVNKGVLAMKRASKIAKENRISDMTLDEINAEIAEARK"
     gene            complement(23019..24404)
                     /locus_tag="PLF14_00785"
     CDS             complement(23019..24404)
                     /locus_tag="PLF14_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HOT69868.1"
                     /translation="MSNSTIINKIVEKERIYFNEGHTLPISARIKSLKLLETSIRNNE
                     DMILSALKSDLNKSRYEGYMTELAIVFCELDHTLKNIKHWARPERKKVALSQAPAKAK
                     IIKEPYGTVLIMAPWNYPFQLCMVPLIGAIAAGNTAVVKPSAYAPAVSNAVCSVISEA
                     FPPEYVSVVEGGREENNALLEQHFDYIFFTGSPAVGKTVMQQAANNLTPVTLELGGKS
                     PVIAEQSADIKKTAKRILFGKLLNAGQTCVAPDYLLVQNSIKAELINEIKNHYNTMIP
                     DTKYKRENFPKIINKKHFDRIRQLMETQDILIGGNTYPDTLQIEFTLVNEPDTESSLM
                     QEEIFGPVLPVIGFDDINEAVNFVKSRPKPLALYLFTKDKSVEKTILKNLSFGGGCVN
                     DTVMHLSSPHLPFGGVGNSGMGRYHGKETFETFSHTKSILKKSWYLDIPVRYHPFQSP
                     DKKLPEWLFKK"
     gene            complement(24428..25252)
                     /locus_tag="PLF14_00790"
     CDS             complement(24428..25252)
                     /locus_tag="PLF14_00790"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOT69869.1"
                     /translation="MEVGLVTRSLANHTPEETVDLLKKFGFTSTEISFAFSNLYVYQY
                     NGYKPMDALTDEAAKDIIKLFRDNSIDVVAIGAFSSLFEPDKEKQENNFKAYERYIQV
                     AANNGIEYVATESGFVPGKRGIDFFTFEADFAYFKSNMIRLLDIAKHYGVSIAYEPCI
                     FDFAPSAKRTRDLIWQCERDNFKILLDPANLIANSDEEDIFKYLKDYIAYFHGKDRKV
                     NDAKGRNVGEGDIDWVKFLSLRKKHTPDAAFILEYCNKTNCEMVKERVLEYAKQVV"
     gene            complement(25268..25801)
                     /locus_tag="PLF14_00795"
     CDS             complement(25268..25801)
                     /locus_tag="PLF14_00795"
                     /inference="COORDINATES: protein motif:HMM:TIGR04518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HOT69870.1"
                     /translation="MSNTRRITILSLLTALTVVLAKLLAVNLQVIRIGTEFVSIAMAS
                     ILFGPAYGALSAVLADLLGSVLFPTGPYSPVITIVAMANGLIYGLFLHNKEITVKTTI
                     FAVLTKALISDFFLMSIALYMLYRIPINELLVARSIKTVAVIVIETLVIFLVIRPVLN
                     IFYKKGWIVPEDIIKKD"
     gene            complement(25798..26796)
                     /locus_tag="PLF14_00800"
     CDS             complement(25798..26796)
                     /locus_tag="PLF14_00800"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT69871.1"
                     /translation="MNQRERFLNVMNFRPVDRLPMIEWAGWWDKTIQRFISEGVPDMP
                     NKEDIKHEYFGLDRLKQYWIGARGRGYNQLSEDMKKVTDIQSYERLKPYLYDKRKVQA
                     AAEDAAQYKKMHDDGDLVVWITVDGFFWFPRTLFGIEEHLYAFYDEPELMHRMNQDEA
                     DYINYVIDEMSKAIQPDFMTFAEDMSYNLGPMLSEEMFDEFLLPYYRQTVPHIEEAGI
                     IPIVDTDGQLEAMIPWLKRAGIRGALPLERKAGVDINRVRKNHPDFLIIGAYDKMVMS
                     VSEQAMREEFERLLPAMRSGGYIPSVDHQTPPEVSLDNYRIYLKLLEEYCIKGAVK"
     gene            complement(26802..26930)
                     /locus_tag="PLF14_00805"
     CDS             complement(26802..26930)
                     /locus_tag="PLF14_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT69872.1"
                     /translation="MSNTGPITILSLLTALTVFFAKPLAVNLQVIKKGAEFVSIAK"
     gene            complement(26927..27925)
                     /locus_tag="PLF14_00810"
     CDS             complement(26927..27925)
                     /locus_tag="PLF14_00810"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT69873.1"
                     /translation="MNQRERFLNVMNFQPVDRLPMIEWAGWWDKTILRFISEGVPDMP
                     NKEDIKHEYFGLDRLKQYWIKPRGRGYYQLSDEIKKVTDDESYERLKPYLYDKYRAQV
                     AVEDAAQYKKMHDDGDLVMWITVDGFFYFPRTLFGIEEHLFAFYDKPELMHRMNQDQA
                     DFINYIIDEVSETIQPDFMTFAEDMSYNLGPMLSEEMFDEFLLPYYRQTVPRVEEAGI
                     IPIVDTDGQLEAMIPWLKRAGIRGALPLERKAGVDINRVRKNYPDFLIIGGYDKMVMS
                     VSEQAMREEFERLLPVMRSGGYIPSVDHQTPPEVSLDNYRIYLKLLEEYCIKGAVK"
     gene            complement(27965..29881)
                     /locus_tag="PLF14_00815"
     CDS             complement(27965..29881)
                     /locus_tag="PLF14_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOT69874.1"
                     /translation="MKKRFLVLSIICILVMSAIYGYAAEEQTFVLTKEEQAVVASYPV
                     VYVALDRNSFPFEFYNYAEKQYEGVVVRILDHVHQLTGFQFEYLKTKDFEQALQYGFN
                     FQADITVSKVNRYAGFMQSRQLVKFKGYDVGMQFTQRMPPDVAALINRAIDTISEEDI
                     KTIIASEAVQYEKMFNRTELRVTIALSVVISAILTGATILIAYFKYKKNKNLFLNTDS
                     LTGYPNIRGMKAVTDVSISMLNISTYCMVNLVVYNLNYIREVYGYTEADKVQNDIAVL
                     LDNHIYNNEILARANNDDFILLLQFTEREEMIGRLSNIVEKVNEKYNLKNSDYPVVLS
                     IGIYNPKYEELNMDVLMQKSLQARQFVKEKDDVRYKFYDDTVRIMTVEEKLMQQEFLA
                     SMEKDEFLIYIQPKILLADNSIYGGEILSRWYNPRRGLIRPGEYIPVFEKSGIITTFD
                     MYMFEKACQLLQQSLKEGRQVVPVSVNFARSHFANIDFCDRIFAIATKYQVSPNYLNI
                     EVTESLIAENAKMMKDIVNKLKEMGFTISLDDFGTGYSSFSDLPNFMLDYLKLDRSMI
                     YNLEDSKTKKMIKGITDLAHNMDSLIICEGVETKEQADILRSLDCDIAQGYYYYQPMP
                     YEEFEKLLQSKQID"
     gene            complement(29882..30325)
                     /locus_tag="PLF14_00820"
     CDS             complement(29882..30325)
                     /locus_tag="PLF14_00820"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HOT69875.1"
                     /translation="MIAFKGIKKVLFGYSKKDVIMYIELLQEDLNSRIAEKDRQVNLI
                     LEQNKMLLNRLELLEKNKDDIALSLIHARKKADNILADARKQADIILKDTMEEKERQV
                     EQYKQVEEEFEEFRQSVSKVLKSFIRGAEGIKKDCDKAHKLSIGE"
     gene            complement(30389..31792)
                     /locus_tag="PLF14_00825"
     CDS             complement(30389..31792)
                     /locus_tag="PLF14_00825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT69876.1"
                     /translation="MFIETARAILISIGIFYSLYLFAYATFLLVSVTIGASELYKSKQ
                     LNTLHNVISHDYYFPVSIVVPGYNEEVTICDTVHSLLNLDYRLYEIIVVDDGSKDNTS
                     KLLIEEFKMTKVRRKIKKSIRCQDEEFIYESYAYKVPIVLIRKKNGGKADALNMGINA
                     SRYPYFVCMDADSMLQSDALERIVRPVLTDENLIACGGVIRIANGVVLENGKVKKYEM
                     PDNMVAAMQVLEYERSFLASRILFDMFNGSLIISGAFGLFKKDMVIAVGGYDHTTMGE
                     DMELVVRLHVFCLKNQIPYTIKYATDAVCWSQAPDNMRDLRKQRRRWHLGLYQSLTKH
                     KQILFSSKFGLVRTVSYMYFLIFELLSPYIEIFGILSVIAMFLLGMVTTRVAIAYTII
                     SLLYGAVLTLSAFFSRIHTQSIRLTAKDVWKAIGACIFENIGFRVILAYTRMVAFVGY
                     KKKKLQWGKIKRVKFNR"
     gene            complement(31782..32912)
                     /locus_tag="PLF14_00830"
     CDS             complement(31782..32912)
                     /locus_tag="PLF14_00830"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOT69877.1"
                     /translation="MANEVRIDLILIIYTAICVMMICFNTVFLLYLRYRDFIFKDKRE
                     KIRQAIKDQIELVSKNEEIDKQKQQYLYRNFTKIRFLKAFHEIVKEQAAVDYESTFTY
                     LKLCKPLFEYLIIEFDNKENSKKGYLAFLVGEFKLCDETVYDFIVSAMISYTTNKSVY
                     VRQNALHALYTSDNGEAILTAMLRMQEMKIKHNNKLISDGLMLFHGDRARLASLLWKE
                     YSRFDVSTRVAIINFIRMSTLDYSEKFLKLLKDEHSHKEIRLAIIRYFGRMTYAPAGQ
                     YLVELLRQKNAEWEYRAIAATSLASYKNDETVNALKEALTSDNWYVRFNASESLVELD
                     VEYVDLVDIYNGPDRYAREILEYKMETERLKRKTRKEKRDVY"
     gene            complement(32902..35055)
                     /locus_tag="PLF14_00835"
     CDS             complement(32902..35055)
                     /locus_tag="PLF14_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT69878.1"
                     /translation="MKKFLIATGIILILALGINYVLFSTNFFIDLHPKTPVTTSFITD
                     EENIYILKNGQYEKFQIKGVNIRSAIPGKFGTDYAIEYDDYLRWLKQIKEMGANTVRV
                     YQIMNVDFYNALYDFNKDNEDPLYLLHGLTVSDYVQNSHIDAKDEDFYKEIIKQTYIL
                     VDIIHGKRQLVLNQQSGYGFYMKDISPWVLGYILGSNWNSNTIAYTDRLHKVHEGYSG
                     KYMYTTEDATAFESVLAMIGDTIITYESARYKEQRLVSFFNWPSTDPFVYPDYITEFF
                     KKCAEVDVEHIRTTDAFISGHFASYHAYPYYPGYLNYFENKDFWFKDAEGNVNTYTAY
                     LKMLKLHHSIPIVITEFGVPTSRGMAHEDTNKGYNQGRNTEYEQGQIIVNTYEQIKSV
                     DCAGSLVYVWQDDWSLTTWNTQHSVDERRCVYWSDAQSSDQGFGILAFDPGEKKVLCH
                     VDGDLSEWFGIVPVGVNGDMSISMQYDEKFIYYLVYKKDYDRERDILYIPIDITRKSG
                     SNYCKQHDVRFDCYADFLIVLNGINDSRVLVQERYDVLRAMYNQYIAGENPHSRPPHI
                     NSPVFNKINLILQLEALEKVNHPGIKAQVYETGILTHGNSNPAHKEYNSLADFHIAGN
                     YIELRIPWNLLNFSDPSNMFIHDDYYEHYGVESIGIDKMYVGIGSADSRGHIIEMFPF
                     SLKGWNKVTYHERLKASYYMIQKMWTDEERTADGE"
     gene            complement(35073..36488)
                     /locus_tag="PLF14_00840"
     CDS             complement(35073..36488)
                     /locus_tag="PLF14_00840"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOT69879.1"
                     /translation="MRRKLIISSIALICLIAVAVSLSYLNYGNRRHHQHIDDVKHVKC
                     NHTDGRFCTHLPLVHINTDGQDIPVLADNSMVIDADLSIFDKDDSNNHLDDQADLHSL
                     ITIRARGNTSLWFDKKSYLIRLIKEDRTENAKKVMGMEAHDKWILNGPILDKTLIRNY
                     ISMNIAGEIMSNAPDVRFCEVFVNDEYMGVYVMMEAISRGKGRVDISRYREDRPFTSY
                     IVRLDRGTVPEEEIDPFTGYTYNMNSICNIVYPARKNINEQIKTYIENDISAFEKALY
                     SYDYADKESGYRKHIDVMSFVDYLIINEFTQNYDAGIYSTYLYRDTKGKIGIGPVWDF
                     NNAYDNYMETPMSATGFSFQNKVWYIMLMKDDYFTDLVIKRYKFLRKTVLSDEYLINY
                     IDKTVKYLGPAIDRNFDKWGYTFLIDKGLLEPAERNLKSYDAALNQLKNYITARGKWM
                     DENIDSIKQYSHDSRNKSFNN"
     gene            complement(36500..37177)
                     /locus_tag="PLF14_00845"
     CDS             complement(36500..37177)
                     /locus_tag="PLF14_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HOT69880.1"
                     /translation="MTKYLLELLEQGKTLTVQEILSHLAVAAVVGVVIFLSYMYTHTG
                     TIYSRKFNVSLLMLTLLTTMVMTVIGNNIALSLGMVGALSIVRYRTAIKDSRDTAYIF
                     WAIIAGICCGAGDYLVAAIGSTVVFLLLLAFGKVRNDNRMLVIVKGARNSERQIEAII
                     FNYYSAKANLRVKNTTELTVEYIYEVSKKVMRKAAANEKSITERLYELQHIEYVNIVE
                     QNDEINN"
     gene            complement(37192..37887)
                     /locus_tag="PLF14_00850"
     CDS             complement(37192..37887)
                     /locus_tag="PLF14_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001006716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HOT69881.1"
                     /translation="MKQVLRQEKKYIISKTDYIKYSAAFNTYLAQDENNGEHGYMVRS
                     LYFDTLWDNDFVEKEDGVEIRRKIRLRIYKPTDQFAGLEMKQKQGSQQLKRSLKMTRE
                     DAILLTKGIYTPLLGYKDPFAKELYAVMNMRFYRPKAIVEYNRKAFIAKENSIRITFD
                     HNIRATESCFDIFSENLNTYPVFSQDGVILEVKYNGFLLSYIKDMLDRVEQSELSVSK
                     YALSRTICLNYVF"
     gene            complement(38020..39411)
                     /gene="murC"
                     /locus_tag="PLF14_00855"
     CDS             complement(38020..39411)
                     /gene="murC"
                     /locus_tag="PLF14_00855"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOT69882.1"
                     /translation="MKAGFLQGIKHVHFIGINGTSMSGLAILLSEKGIKITGSDIEHT
                     ATMDKLLSMGIHVYIPHDASKVGKPDLVVYTAAVHPDNCEYAYAVSKEIPVMDRAVLL
                     GKIMEEFKYSIAISGAHGKTTTTTLAAYLLEKMGYDATIHNGGEAEFLNGNVKSGNSD
                     YFLTEACEFNGSFLKLKPYIGVILNIDFDHVDWFKTFDNMKRSFLEFADKIDKDGFLV
                     ACYDDIPTREVAKKASRNVVYYGLNNLSLDYTAMNMTIDTQGKPSYDLYIKGNFICHV
                     QLGAEGQHNALNSLAVIAVMGLLGADLTEVCTHLSFVHGAKRRFDLIGEADGIKVYSD
                     YAHHPTEIAVTLETASRMKHNKLYAAFEAHTFSRVRVLFEEFVQSFALADIAIIADIY
                     NDRESPDSEVSGKRLAQAINKNVTKAIYLPSYQAIEDYLFEHAKSGDIVMVLGSKYLE
                     TICKPLLKRLEKR"
     gene            complement(39414..39779)
                     /locus_tag="PLF14_00860"
     CDS             complement(39414..39779)
                     /locus_tag="PLF14_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002599232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HOT69883.1"
                     /translation="MKKDIVCIVCPRGCTIQVNYDKNGIISMEGSSCKRGDEYARAEI
                     LSPTRMITSSVKIDNAKYTVCPVKTGAPVSKSKIFDVMEEIYKVRIQAPVQQGDVIIK
                     DVCGTGADIIATQTFEAES"
     gene            complement(39776..41053)
                     /locus_tag="PLF14_00865"
     CDS             complement(39776..41053)
                     /locus_tag="PLF14_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT69884.1"
                     /translation="MKQFNLAIIGGGPAGMAAALQAYELGVKDIVILERDNSLGGILN
                     QCIHNGFGLHVFKEELTGPEYALRFSEKIKETNITVMLDTMVIGAEKDKTLYAVNPEY
                     GFMVIKAKAVILAMGCRERPRGALRIPGSNCAGVFTAGTAQRFVNIEGILPGKKVVIL
                     GSGDIGLIMARRMTLEGIKVMGVYEIMEYPGGLTRNIVQCLDDYDIPLHLSETVVKIH
                     GKGRITGVTTAKVDSNMKPIKGTEQYVECDTLLLSVGLIPENELSEQMGVGMYQVTGG
                     PFVDQDLETDTEGVFACGNVLHVHDLVDYVTMEGQRAAMSAKAYLDKTLTEKKSAQKI
                     AVVAGNGLRYVLPQHIYLKDTEEKIKIFFRSRARFREVYVRASVGDNVLTRRKLLRLI
                     PSEMESVDIKMRDLLNTGSETNGFGDLVIEVEQ"
     gene            complement(41049..42542)
                     /locus_tag="PLF14_00870"
     CDS             complement(41049..42542)
                     /locus_tag="PLF14_00870"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOT69885.1"
                     /translation="MYYDICIIGAGIVGSLIARQLSKYKVKIAVIERHVDVSMDATGA
                     NSGIVHAGYDAKPGTKKAKFNVSGCNMMPGLCDNLSVKFKKTGSFVLAFDQEGEEVLQ
                     KLYDSGIKNGVTDMAIVDKDFILKREPYVNPLVTKALYAKNAGIVSPYKLNIAAMDNA
                     VMNGVKFLRETEVTGIEKEDGLFHISTEITGDICSISKLDRIKEEDRTDITAKIVINC
                     AGAHADEISRMAGDDSFNIIPRRGEYILFDKTEGSRINTVLFQTPTEKGKGILVTPTV
                     DGNLMIGPDATVADHKDDKCTNSENLDYVFKTASQTYTRLNIRKSITVFAGIRATPDT
                     SDFIIEESKKCGNFINVAGIESPGLTAAPAIAVYVEDMVKSLMEMKEKAKNQYTMKVK
                     HDKPFSEMTNEEKEAACKSDELCRKIICRCETVSEREIVSAIYAPCGARTVDGVKRRT
                     RAGMGRCQGGFCAPHVMRILSEHTGIAVNKIAKADADSCILGRHSRD"
     gene            complement(42706..43860)
                     /locus_tag="PLF14_00875"
     CDS             complement(42706..43860)
                     /locus_tag="PLF14_00875"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT69886.1"
                     /translation="MRDFEQLKKMTADFYSMKPDAPIPLEEFGYYSKEQWISQGYIKE
                     GEDMVALFGFERLTNYYLHGLAGTVAPFVPRFKEEVIHSDEQYEIAIDKAGRTVKYFK
                     GRRDGFMPQYLDFPVKDMATWEEIKWRLDYANAQRNEDLKKFISDSVPLAEKGYHSVM
                     YIVGAFMYLRSLVGGEEILYMIYDDPGLLHTCMEKWLELSDYVTAKYQDHVRIDELLL
                     DEDICYNHGPLISPEQIRTFLFPYYKELIQGLRSRNARLAMSDKECAYKQPKTYDLII
                     HVATDGDCRPVIPLYKEIGMNYMSPMEVASGCDVVQIRKDYPDIRIKGGIDKRILAHS
                     KEAIKAEIDRILPFMKKQGGYLPTCDHGVPPEVPFENYVYFRELLKDYAR"
     gene            complement(43871..44917)
                     /locus_tag="PLF14_00880"
     CDS             complement(43871..44917)
                     /locus_tag="PLF14_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT69887.1"
                     /translation="MEFTPDYRNIQDAAFNKKAKRLPMYEHSISTDHMEKILNKKFAA
                     LIDGDFSDKCEFFNRYCLFFKTMGYDTVSFEVAIRESFPGGGALDKHITPAITSREDF
                     INYPWEEIPELFLKRADEFYMALKQSLPPGMKMIGGPGYGIFECVQDLTGYMNLCIMS
                     YEDPELYADIFLAMRKLFVNIWQGFLDKYADMCAVARMGDDLGYKSNTMLSAEDIKRY
                     IVPGYADIVRLSHSYGLPFLLHSCGNIFNVMEDIINVAKINAKHSNEDQIAPFSVWVE
                     RYGDRIGNFGGVDADVLCRTDKQQLKEYITDLLYSVGNEKGIAIGTGNSVPDYVPTEN
                     YINMIETVREYRKG"
     gene            45060..46532
                     /locus_tag="PLF14_00885"
     CDS             45060..46532
                     /locus_tag="PLF14_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020617497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOT69888.1"
                     /translation="MTTLKMTVNTTVKKGHISPLIYGSFAEHLGRCIYDGIYVGDNSD
                     IPDTDGIRNDIVEALKAIKLPVLRWPGGCFADEYHWRDGIGDKSKRKKMINTHWGMVI
                     DDNSFGTHEFLRLCELIGCEPYIAGNVGSGTVREMAEWVEYLNCAAPTSVVLERAANG
                     RIEPWKVKYFGIGNENWACGGNMRPEYYADLYRRYAVYCRNYGSGRLYKIACGPSSHD
                     YNWTDKLMESITATKDCSAISLHHYCLMTNVRATELSSTDYAKTIAKAQAMDKIIEKH
                     WEIMGKYDPDRRIKLVVDEWGTWYRSEPGTHPSFLHQQNTMTDAIVAAGTLDIFNNKC
                     NIVDMANIAQTVNVLQSVILTSGKQLIKTPTYHVFDIYKEHQNNMLLESSCENRIFNS
                     DEEIYAVSHSASLKPDGSVFLTLSNADPDKAYETDINLIGNNYDNISAEILVSDNYLS
                     HNTFTEPEKIKKQTYTQYKLKNNRLLLTLPACSVVGIALK"
     gene            46664..49615
                     /locus_tag="PLF14_00890"
     CDS             46664..49615
                     /locus_tag="PLF14_00890"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HOT69889.1"
                     /translation="MDKLQSLYDEINGNTESPVAYMPKTPITSRFVSPWDTWGWYTLK
                     SNFRKGVALYSNSDDYVKNIDDCYAGADYIQTFNSKAVNLNDHPELDFFVETDASVTV
                     AMEEGCMPEWLKDWTNTKKSMTSGKGIKYLLYTKEFPKGAHVHVPGFETDHNHYIVII
                     LPLSNREKLSKTDKIHYPNTQLQPHKTRLYQSYIVEVFNDKNDGIFVSNDYRSFGCCH
                     IKTDDKDRKNKYLALETTDKCDKAYVKKSVGINIEYPIVFECKLNISKDSAMQALLTG
                     SNEKSIGAIFKKDGFIYDAEGKIKVCAFTKNTDVCLKIKADTQSKTYEIWVNHVKQAK
                     NIPLDMEDIQHMCFHVQSDKSLSYAYVDNIYLYDDTEIYAVNETFETDTLNNWTSNGK
                     LAIKPYPFDKDRSLTLTGASYATYAFCPVDDIVSIETKVKVADESFTLAPEIADKCGN
                     VAVKVALYKNNLYASDGEVWKRIYEGLTPWMYYPHNNWFNIKVTADIKKNTYDLYVDG
                     AKRAVGFRFINKTNNLGQLAFTCEKSSKVYINRIRIYDCADFSRGVLPNAKVFDVKSA
                     PYNAKGDGKTLETAKIQKAIDDAAYTGGTVYIHDGTFFTGGLILRPDMTLFVDRSATI
                     IGTQDHSQYKLVSPGISLCAVRQLGRGLLYGENISNIRITGGGTLDGNGTYRYKMNDP
                     LQDREADARPDIVYISYSNDIVVENVDMKSSAFWTVVPLSSGNITIRNLNLDCMNTPN
                     RDGIDPVDCHDMTIYNCNIMAGDDGLCFKSSDNVGCYNIDAYDMMIQSLASGIKFGTD
                     TYYCLKNARIRDCAIKNVNRCGVSLESVDGAAVENVIFERLDMTDVGAPLYISTGIRN
                     RLPRGNQPVRRSYMKNVTFKDIRFEQPYPFSFEREIRENMVIGQSKDNLIENVNFINF
                     DLKLPGGVKTLPKPPVVINDKYPEYDRHGLSSGYAFTIKYAKNVKFKNLKVTLENEDI
                     RDEVAYFDYEE"
     gene            complement(49623..50852)
                     /locus_tag="PLF14_00895"
     CDS             complement(49623..50852)
                     /locus_tag="PLF14_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT69890.1"
                     /translation="MNYIKRHMEEVVLRLSEQFPAILITGPRQVGKTTMLKELMKSEP
                     ENRTYVTLDDISDRMLAKTDPAMFFQLHKPPIFIDEVQYAPELFPYVKILIDKGCNPG
                     DFWMTGSQIFKLMRGVQESLAGRVALLQMLPLSQSEITGGVTVPFTTDFDTLLERQKA
                     VASVSAPELFERIWKGGMPALVSGKYKDRSVVYSGYIKTYLERDVSDLSEAIDAMKFM
                     RFITAVAARTAQLVNYKGIADDAEIDQYTAKNWLNILETLGIIFYLHTYSNNVLKRTI
                     KTPKLYFFDTGLVCYLTKWSSSETAMNGAMSGALLENYTIAEIIKSYQNCGREPFIYY
                     YRDRDSKEIDLIIEGDGKLCPVEIKKAAMPDKRLVKSFSIIDKSPLKRGTGAILCLAE
                     KLGAFDKDNLIVPIWLI"
     gene            50987..51337
                     /locus_tag="PLF14_00900"
     CDS             50987..51337
                     /locus_tag="PLF14_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT69891.1"
                     /translation="MIFLDAVIANPDRHTNNFGLLRDINTGTIIGLAPIFDHNMAVTA
                     RGYPGNPKATDLLISLFNDLMKKYPEYTTHIPSVTEQTVINILDKINMRVKRQVIIDL
                     VMGRYGFIEQNNID"
     gene            complement(51365..52012)
                     /locus_tag="PLF14_00905"
     CDS             complement(51365..52012)
                     /locus_tag="PLF14_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT69892.1"
                     /translation="MTYIEYLCKIIRKIQPGEPIYSKQLSESVKDAFKLNYKDASAAV
                     AVAMKRIIENRTIPDLRSYQKGIFYRTTETVFGEKGINKNKLISDKYLANNNGYETGP
                     ALLHRLGLTSQMPKHPVLATNVARDCMRYDKKLGVAIKVPKIKVTADNKMYLQILDVL
                     DMMDSAPLDEDQPYSVVANHIKKQNLRYDVLLALADKYYNQKTVLQLARTASAGA"
     gene            complement(52225..53316)
                     /locus_tag="PLF14_00910"
     CDS             complement(52225..53316)
                     /locus_tag="PLF14_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016992207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT69893.1"
                     /translation="MYEDKRVDTKRETDQKCPSCGGTMDFNPKTGGLTCPYCGYTRKI
                     SVKEAEDRKVEEKDFLAEEKKGNFNWGAEKKTVVCKSCAAVSVYDALQISDVCPYCGS
                     NQVTEEKGADSLAPDGVCPFTVTDKEAGANFHNWLKRKLFCPKAAKEGAKPESFKGVY
                     LPYWTFDTEADVKYTAEYGKEKEVKDDEGETKKVMEWYPTRGTYEKFIDEYPVPATER
                     YDQGTLNMIKPFDTENSIPYKPEYVAGFISERYSVGLQKGWNIAKEDIESILHSEITD
                     KIEEENDADDVRKLDMKIKYNDIKYKYLLLPIWLSSFKYKEKIYRFMVNGRTGKVGGR
                     SPVSPARVALAIGIVIAIILLILKLSGNI"
     gene            complement(53327..54637)
                     /locus_tag="PLF14_00915"
     CDS             complement(53327..54637)
                     /locus_tag="PLF14_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOT69894.1"
                     /translation="MGLIRAVVGAAGGVLADQWREFFYCESMDADVLVKKGQKRTGKR
                     GVNVKGEDNIISNGSIVAVNEGQFMMIVDQGQVVDFSGEPGEFIYDTSTSPSMLYGNF
                     GEGLLNSFKEVGKRFTMGADPGKDQRVYFFNMKEIVGNKYGTPNAVPFRVIDKNIGLD
                     IDIAIRCHGEYSYKIVDPVLFYTNVTGNVVDEYRREVIDGQLKTELMTALQPAFAKIS
                     EMGIRYSALPGHTMELADALNQVLSEKWTQTRGIAIGTFGISSLKASEEDEELIKKLQ
                     RSAVMRDPTMAAATLVGAQSEAMVHAAQNEGAGPVMAFAGMNLAGQAGGMNASNLYAM
                     GGNQQGPNAPDPNSWTCSCGTVNKGKFCGECGKPKPVEQAGWTCECGTVNKGKFCGEC
                     GKPKPAGAKTYKCDKCGWDLPAGATPPKFCPECGDAFNDADEVK"
     gene            54934..56250
                     /locus_tag="PLF14_00920"
     CDS             54934..56250
                     /locus_tag="PLF14_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT69895.1"
                     /translation="MKKTILLFLLVCIIVLAGCQKQPIKTDQPTTSIPSQTQTPTPSS
                     EVTQTEEPPEKFNLYEDTKGMAVVKIQDGEASIAINPKNWSDVPENLDRDKQTAFCKI
                     NGLAGKISTACVGEISDLSQTFGGDGPFTTPIAVFLMEDGSLEWVTVNPWWYVSGAEQ
                     GYDKVAHSWGKIPWMENITSLSFGSENDGDGSRTTIYATDKDGLRYNLKYPCNFQSLL
                     HTTWRYTIDPEDKDALYLTFEDFERVDLDLRWGAQPLENFFGTYKLYLAEDGKQGKQA
                     GCIEFDFTLGWWIYEGEGEIDRPTAIKGTFFTQFARDWSLSLTLWPADGDPLYATREK
                     TPDEYRFLSADMLEAIPEYTMDDDELVIYLMYIVPDVNAMIKESGMSALVTGETTELA
                     GGVVCRNIWLGTKKDDSFTRELLYGVSPDGSVYRYDALSDSWTMLH"
     gene            complement(56414..56490)
                     /locus_tag="PLF14_00925"
     tRNA            complement(56414..56490)
                     /locus_tag="PLF14_00925"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56454..56456),aa:Pro,seq:cgg)
     gene            complement(56549..57298)
                     /locus_tag="PLF14_00930"
     CDS             complement(56549..57298)
                     /locus_tag="PLF14_00930"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HOT69896.1"
                     /translation="MSEDKKKKIRRRNIKLIIASAIILAFLIFILTEKGKQLWDLFQL
                     NGEQFKEYIDSKGKWGPALFFLLSYLQVVIAPIPGQITYFVGGMLFGTWGGFAVNHTA
                     TVLASITVYWLGRAFGEPLTEKLAGGGKTKKVVDFVKRNGPIFFLLAFLIPGLPDDLL
                     CYVAGIVMLPFSIFLFLALVARIPSTLSLAFLGDGVLKWTTGQMILVIVIMLVLIGLV
                     IIFRKPITNFLNMLVRKESKDDDVDITDMMT"
     gene            complement(57295..58149)
                     /locus_tag="PLF14_00935"
     CDS             complement(57295..58149)
                     /locus_tag="PLF14_00935"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOT69897.1"
                     /translation="MIITGISDEAGKHITTQIEAHKKLGWNTLELRNHNGVNPSTTNF
                     SDQEFDNVRKIIEEHNFKVTAFSSAIGNWSRSIRGDFNIDKEDLKISAKRMKMLNVRY
                     IRIMSWIPDEDDKKYTKKEVIRRCKELAKMAEDLDVILAHENCTGWGGETPENMLELK
                     QEVNSPNFVLLYDTGNVVAHGYDKPMKFFQGLRGNIDYVHIKDALIARPGLENQFRYV
                     GEGEGKVREILDILINEDKYDGVISIEPHVAAIVHTGEKPAGDEELFQSYIKYGNMLK
                     EMIASIKR"
     gene            complement(58146..58514)
                     /gene="rsfS"
                     /locus_tag="PLF14_00940"
     CDS             complement(58146..58514)
                     /gene="rsfS"
                     /locus_tag="PLF14_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOT69898.1"
                     /translation="MDTENFKNEIVEVLKEKKAKDLISIEVRDVTTLTDYFVICTGTS
                     DRHRKSMADDVQEKAEALGMTVSMPVEGYNQSKWILIDCMDVVVNIFEKETRSRYNLE
                     KLWSDGVLEEIRENNKEEKK"
     gene            58655..59302
                     /locus_tag="PLF14_00945"
     CDS             58655..59302
                     /locus_tag="PLF14_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT69899.1"
                     /translation="MEENCINELLKVTLIFIGIFIIQSMVYWGTQFLVKKPHMLGSKL
                     DDKIPFIPVFIFPYVIWFVLLFTIQFVLYFYSLQTYALYVMSLLIVDITSGIIYLVYP
                     TTFIRPEPEGDSFSVKVVRFIYSHDKKILSCLPSLHCSLSTLFILSALYATGLPLFLK
                     LIIIIFSLLVIVSTVFVKQHVIIDVITAIPLAVISWYIAMAIGADTFLTFFNFLQ"
     gene            59356..59784
                     /locus_tag="PLF14_00950"
     CDS             59356..59784
                     /locus_tag="PLF14_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT69900.1"
                     /translation="MKTAIIYYSYSGKTKKYCENMAAELSADILEVQTVKRKCFLSNL
                     FTECPKALQQQPTDILPIDLDLSGYEKIILAAPMWAGFPAPPFNNMVAMLPKGKQVEI
                     YIVSASGETDPIKKEKVIVLTEETGSKVIKYTDICGADIK"
     gene            complement(59786..60541)
                     /locus_tag="PLF14_00955"
     CDS             complement(59786..60541)
                     /locus_tag="PLF14_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT69901.1"
                     /translation="MWAQNNAKRWVIMSIIFMLIGIAMIVAGAVIGEMNYYWMIYVGA
                     ILFLTFFICFFMFIRQARNLDSMLKGTGLLAHWRFEENERLQRAAEEFLQRKTSNKVM
                     LLVIIAFVVIIGGLFAIFGFSNSKDAIFFIVVLLGIQILISLAAILIPRARYNKMKEA
                     APEVLISPFGAWIMGEYFQWKAPLTRITSVQFAKDREDNYTITVNFTIWQRFGPQKLQ
                     CRIPVPKDTVTEAETIASDIAKINKVRFLNETK"
     gene            complement(60545..61504)
                     /locus_tag="PLF14_00960"
     CDS             complement(60545..61504)
                     /locus_tag="PLF14_00960"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOT69902.1"
                     /translation="MYTGIFKNAYKVIKTEEGYFPVRFTDKQLEYYKNLSKATEDRSL
                     CTAGICMDFYTDAECITFSYRADHFSRRYVGFDFYENGIFKRHIEEALDAKQSTLTYP
                     LTEKAIKRVTVYVPNLSRIRVYDLDIGNFQCIKEQPSQKLLFLGDSITQGMVALSPSL
                     AFPTQVARALNAQSLNRGVGSAKFDVDSLDEQDKYDPDSIFIAYGINDIYQLKDIAEL
                     ENVLKTADKYLYKLKNIYKHAKVYIITPIWCLRYDEDQSFKEMFDEYQKELTTLAKKY
                     GFIPVNGLALMPHDTKYHQDHTVHPADSGFNHISENLLKIIGR"
     gene            complement(61504..62079)
                     /gene="yqeK"
                     /locus_tag="PLF14_00965"
     CDS             complement(61504..62079)
                     /gene="yqeK"
                     /locus_tag="PLF14_00965"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HOT69903.1"
                     /translation="MGYISEEARLDIVKRYSPERYEHVKNVVELADHLARLHGADSEK
                     VYAAALVHDIAKDMKEEDMAAILEAAGIFIEKQYHTGNILHGPCAAVIAKKEYNIEDE
                     EILNAIYYHSFGRAGMGLTEKIVFIADAAEKGRTYKNVTKIRKLMYNKLDEAVELSLE
                     GTVYHVLSKGEVLYPVTAAALEYFQEQRIKD"
     gene            complement(62063..62662)
                     /gene="nadD"
                     /locus_tag="PLF14_00970"
     CDS             complement(62063..62662)
                     /gene="nadD"
                     /locus_tag="PLF14_00970"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOT69904.1"
                     /translation="MKLGILGATFDPVHKAHILMAKYALDYCEYVLLTPCTKPPHKDN
                     AGITDDNIRYTMLCLALDGYEKLIASDMELKRATTTYTIDTLKQVKKEYPIFEKIIYI
                     IGADTLFQLKTWREYEEVFKLCTFLVFGRSGFTEEQITEQIEIFKKDYGADIIFDIRC
                     ISEISSTAIRKGIAEQDNSIEWMLDKKVYRYIKENGIYK"
     gene            complement(62659..63915)
                     /gene="purD"
                     /locus_tag="PLF14_00975"
     CDS             complement(62659..63915)
                     /gene="purD"
                     /locus_tag="PLF14_00975"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOT69905.1"
                     /translation="MKVLVIGGGGREHAVVKKLSESPKIKKIYCAPGNAGMGKIAQCV
                     NIKATDIDGILDFLKDNPVDLVVVTPDDPLALGLVDKLNERNIRAFGPVAKAAEIESS
                     KAFAKNLMKKYGIPTGAFEVFDNPEKALQYVKNKQYPLVIKASGLALGKGVIIVENYR
                     QAEETINQMMINDMFGSSGHTVIIEEFLQGPEVTVLAFTDGKTIKTMPSCQDHKRVFD
                     NDKGPNTGGMGAFSPSKHYTKEIEEYCINNIFYPTIRAMKKEGRNFKGVIYFQMMLTK
                     DGPKVIEYNARFGDPETQVLLPLLKTDLLDIFNAITDERLDKTDIQWENSAGATVVLA
                     SGGYPKEYTKGYPISGLEEIQKQEITVYHMGTAMKEGEYVTNGGRVLSLTAKGDTVEQ
                     AVARVYNNINKVCFKDMHYRKDIGRK"
     gene            complement(63928..65472)
                     /gene="purH"
                     /locus_tag="PLF14_00980"
     CDS             complement(63928..65472)
                     /gene="purH"
                     /locus_tag="PLF14_00980"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOT69906.1"
                     /translation="MIRRALISVSDKSGIAEFAKRLSDMGVEIISTGGTSKVLSENGI
                     KVIPIESITGFKECLDGRVKTLHPNIHAGLLAIRDKKDHMDTLESLDITPIDMLVINL
                     YPFKKTVQKSNVTKEEAIENIDIGGPAMLRSGAKNHATVTVITDPSDYESVLNELEQN
                     GKVSYETNFRLAIKVFEHTANYDAMIADHFNKLTGDYSLKNTFTVTYEKLQEMRYGEN
                     PHQKAAFYKEPAITKGTICDAVQLHGKELSYNNINDADGALSMLKEFPDRPACVAVKH
                     ANPCGVGVGKNVFDAYKKAYDSDPVSIFGGIVAFNRTVDVVTAEKLNEIFLEIVIAPD
                     YKDEALTLLRKKKNLRILRLDNITYNNENEYIMKKVGGGMLVQSKDNCVVNPEDIKVV
                     TVKQPTEQQLKDAVFGMTVCKHVKSNAIVLVKDLASIGIGPGQMSRIGAAKIAISLAG
                     ERAKGSVMASDAFFPFDDCVIAAKEAGVECIIQPGGSLADKEVIAKADQFGICMIFTG
                     IRHFLH"
     gene            complement(65474..66526)
                     /gene="purM"
                     /locus_tag="PLF14_00985"
     CDS             complement(65474..66526)
                     /gene="purM"
                     /locus_tag="PLF14_00985"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018674287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOT69907.1"
                     /translation="MKDAYKEAGVDVQAGYESVALIKKHIQRTVTEGVLGGIGSFSGL
                     FQIDLNKYPTPVLVSGTDGVGTKLKLAHSMKVHDTIGIDAVAMCVNDVLCQGADPLFF
                     LDYIACGKNYPDIIEKIVKGVAEGCIQAECALVGGETAEMPGFYKQDEYDIAGFCVGA
                     VNKPDIIDGSLIEEGDVLLGLASTGVHSNGFSLVRKIFGEDAKSLSKVYPGYEATIGE
                     VLLTPTKIYVKDIKRIRTVAKIKGMSHITGGGFVENIPRMFKDNNLTPVINKGSWAVL
                     PVFDLIMKQGNITEEDMYRTFNMGIGTVIAVSKEDAKKIQKQFKNEIYEIGYVALAGD
                     VKKLKDIEENIYIKER"
     gene            complement(66540..67907)
                     /gene="purF"
                     /locus_tag="PLF14_00990"
     CDS             complement(66540..67907)
                     /gene="purF"
                     /locus_tag="PLF14_00990"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006877192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOT69908.1"
                     /translation="MSGVFGVYSKDKSVNIAEQIYYGLYALQHRGQQSAGIAVRDKDL
                     IEWHKDKGLVPEVFKSQDLDKLPGDAGIGHVRYSSSGDVSKGNIEPLVLKYISGYMAI
                     AKNGNIINANKLRGKLEADGCMFHTTNDTEVLAALLTKFRISSEHIEDSVEQMVKYIQ
                     GSYSIVMITPRFLIGLRDPLGIMPLCIGRINGATYVISSESAAIDAVDGEFIRDVEPG
                     ELVLIDSDGIQSRRLFDCKKSSLCIFEHVYFARPDSIVDGASTHDARVNAGRILAKEH
                     PVQADVVIGIPDGGTDMAYGFAQESNIVAPAGLLRNKYVGRAFIAPNQNAREIAVKIK
                     FNPVKTEVKGKRVVLIDDSIVRGTTTKTLVRMLREAGAKEVHLRIGSPPYKYPCYFGV
                     DTSSRGQLLASKNDKETICEWIGADSLEYISYEGIIKAPVNSKVDFCTSCFTGVYPVD
                     IPKEF"
     gene            complement(67909..68391)
                     /gene="purE"
                     /locus_tag="PLF14_00995"
     CDS             complement(67909..68391)
                     /gene="purE"
                     /locus_tag="PLF14_00995"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOT69909.1"
                     /translation="MKVLVLMGSDSDSKTVEPGLKFLEEYNIEYSVRVCSAHRTPDMA
                     KQLAVNAKSEGFKVIIAAAGKAAHLAGVIAAHTVLPVIGIPVKTSDLGGMDSLLSTVQ
                     MPAGVPVATMAIDGGKNAAILAAEILALNNEELSNKLQQFKKEMEQKVMQADREINNK
                     "
     regulatory      complement(68446..68545)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            68708..69994
                     /locus_tag="PLF14_01000"
     CDS             68708..69994
                     /locus_tag="PLF14_01000"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HOT69910.1"
                     /translation="MSTKELKRLFVLEQSVEGKLTVKEAAELLHLTERRIKQLKKEFR
                     EKGVTAAIHGNANKPSPKRTDKTTEERILGIRKKEPYCSSNFNHFREILKEHFDIRIS
                     YSALYRLLTEHGIKSPKKRRKKRRVYKTRERRPAFGMMLQADASPFNWFGGEKKFSLH
                     GLIDDATGKLTGLYLCENECLLGYLEVLRQTLISFGIPQSLYPDRHSVFFYTPQDKDK
                     LSIEEQLSGCTKKVTQFGKIVEQLGIDMFPAQSPQAKGRIERSWNTLQSRLPVELKMR
                     GITSMEKANLFFEKEYINLFNEQFSVEPKESYSAFVPIPHTIDLDRLLSVKLERSLSS
                     GSTISICNKLFKIEQDRFSAREKVTVLLSKKHGLRALISGEFYPIYPIDDISYAEDVV
                     RTGDFPTVVVSLLHHFFLSDVRLGHFPEGVKYSQNN"
     gene            70329..71243
                     /locus_tag="PLF14_01005"
     CDS             70329..71243
                     /locus_tag="PLF14_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOT69911.1"
                     /translation="MKKKTNYCKIKWEELRMKKTITIVISLVLLLAMLGGCTQKGFDN
                     KTAITVIAREEGSGTRGAFIELFKIEKKDANGKKVDHTISTADINSSTGVVLTTVAQN
                     KYAIGYVSLGSLNDTVKALQIDGVAATVANIKNGTYKVSRPFNIATKDGLSDVAKDFI
                     DYILSKEGQAVVEKSGYISAVSGEAYAGKKPSGKVTVAGSSSVTPVMEKLKEAYIKLN
                     PNAVIEINQSDSTTGVNSALDGICDIGMASRALKDSEIEKGVKPTVIAIDGIAVIVNN
                     DNPTVGMKAEEVTKIYIGEITKWSEIGN"
     gene            71287..72114
                     /gene="pstC"
                     /locus_tag="PLF14_01010"
     CDS             71287..72114
                     /gene="pstC"
                     /locus_tag="PLF14_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015533776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOT69912.1"
                     /translation="MKAIFFISAFMSVVAVILICIFLFANGLPAMKKIGFAEFLLGRT
                     WRPGNDIYGVFPMIIGSLYVTAGALVIGVPVGILSAIFLARFCPPKLYKVVKPIINLL
                     AGIPSVVYGFFGLMVIVPFIRNTFNVAGTSMLAASLVLGIMILPTIIGMSEASIRAVP
                     DSYYEGSLALGASHERSVFYSLLPAAKSGILSSVILGMGRAVGETMAVVMVAGNQPVM
                     PSGVLKGLRTLTANIVLEMGYAADLHREALISTAVVLFVFILIINLLFSIIKKGVKS"
     gene            72111..72956
                     /gene="pstA"
                     /locus_tag="PLF14_01015"
     CDS             72111..72956
                     /gene="pstA"
                     /locus_tag="PLF14_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOT69913.1"
                     /translation="MKAAKRQSVFLRCLVYISAGLTITTVVGILIYILIKGVSHLSWD
                     LFAFKYTSENVSLFPALVNTVTMTVVSLLIAAPMGIFSGIYLVEYANRDSKAVKLIRL
                     TTETLSGIPSIVFGLFGYLLFVSSASGFGWGYSMLAGAFSLAIMILPVIMRSTEEALK
                     SVPDTYREGSFGLGAGKLRTVFKIVLPAAMPGILAGIILSIGRIVGETAVLIYTSGTV
                     AHIPKNLMGSGRTLSVHLYALWSEGMNTESAYATAVILVVTVSLLNALSAYLAKKFTK
                     GNLNG"
     gene            72949..73698
                     /gene="pstB"
                     /locus_tag="PLF14_01020"
     CDS             72949..73698
                     /gene="pstB"
                     /locus_tag="PLF14_01020"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOT69914.1"
                     /translation="MDKFVIENLDLFYDTFQALKKVNMSIPMNEITAFIGPSGCGKTT
                     LLKTLNRMNDLIAGCKIQGKVLLDGVDIYGDIDVNLLRKRVGMVFQKPNPFPMSVYDN
                     IAYGPRTHGIKAKIKLDEIVEKSLTDAAIFDEVKDRLKRNALELSGGQQQRLCIARAL
                     AVEPDVLLMDEPTSALDPISTAKIEDLVLELKNRYTIIVVTHNMQQATRISDKTAFFL
                     LGEVIEMNDTEILFSNPADVRTENYITGRFG"
     gene            73698..74342
                     /gene="phoU"
                     /locus_tag="PLF14_01025"
     CDS             73698..74342
                     /gene="phoU"
                     /locus_tag="PLF14_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005359795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOT69915.1"
                     /translation="MRSKFDMQLEILNNSLIEMGAMCEKAISSAIKALLENNISLAKK
                     VMESVKDINQAEKDIEAMCLKLLLQQQPVARDLRQISSILKMITDMDRIGVQAGDIAE
                     IVTFANLSKNKDRVHIADMAEATIKMVTESINAFVKKDLELANKVIAYDDVVDDLFVK
                     VRNGIVDLISKDKRHGEQAIDIIMIAKYLERIGDHATNIAGWVAFSITGIHVEL"
     gene            74345..75031
                     /locus_tag="PLF14_01030"
     CDS             74345..75031
                     /locus_tag="PLF14_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT69916.1"
                     /translation="MIYFVEDDSSIRELVVYTLNNTGFEAEGMDNAKTLYSKISVNKP
                     ELILLDIMLPGESGLEILKKLRSSKETANIPVIMVTAKGTEYDKIIGLDSGADDYISK
                     PFGIMELVSRVKALLRRVSRKEDTDSVTDTYTYEGVCINSKNHTVTLDSEPVVLTLKE
                     FRLLYELMANQGIVLTRDTLLERVWGYDYPGETRTIDVHIRTLRKKLGKYGDIIKTIR
                     GVGYIIGGNA"
     gene            75028..76704
                     /locus_tag="PLF14_01035"
     CDS             75028..76704
                     /locus_tag="PLF14_01035"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT69917.1"
                     /translation="MRKRVFISIFLTSLLTMILTFILISWIMYNNTFDSVKREVQNES
                     FYVSDALKTYNNDTADMFSYLNRAGLKSRNRITYIKSDGTVLYDNLALAADMENHSDR
                     PEVIEALQTGHGEITRYSDTLGESIYYYAIKLEDGNILRIAATTKSILGMMTGSAIWI
                     ILIFITFISLSVIIARLLTKSIVRPINNLDLDNPLSNKTYDELSPLLLRLDKQKTELM
                     ENVQEISKTRINFAFISNNMSEGIVVFSEKGYITGANRSARDILGIDIQQFFMEGCRD
                     LGYIKAVRSALEGKPMTAKFEKSGFVYQLSASPVKENPEKHAALLFITDITEKDQAEQ
                     RRKEFSANVSHELKTPLTSISGYAELISKKIAKKEDVPRFAEMIHTEATRLLQVIEDI
                     MKLSRLDESDLEKEFEDVNLSKLCTSVINDLQQIAQNKDVVIKSDLKNVYINGYSPIL
                     YEMLYNLIDNAIIYNNPKGYVDISLAEEEDEIILTVKDNGIGIPPEHQPRIFERFYRV
                     DKSHSRDTGGTGLGLSIVKHAAALHKADIDLTSTPGEGTTVTVTFYNGDK"
     gene            complement(76718..77563)
                     /locus_tag="PLF14_01040"
     CDS             complement(76718..77563)
                     /locus_tag="PLF14_01040"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HOT69918.1"
                     /translation="MERQKRVLAIHDISCFGRCSLTVALPIISAAGIECTVIPTAVLS
                     THTGGFTGFTYRDLTEDIMPIVEHWKTLDIWYDALYTGYLGSFEQIDIVSDIFDIYKN
                     KDTLILVDPVMADNGKLYASFSPDFPKGMARLVSKADAVVPNITEACFMLDKQYKEGP
                     YDERYIRDLLLGLADMGPDKVVLTGVYYNEKELGAATYDKTVDQFGFYKRERIEGYYH
                     GTGDVFGSALLAAMIKGKNLIDSATVAVDFTVESLIRSYKAKTDIRFGVNFEAGLEKL
                     AKELK"
     gene            complement(77710..78075)
                     /locus_tag="PLF14_01045"
     CDS             complement(77710..78075)
                     /locus_tag="PLF14_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005949272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOT69919.1"
                     /translation="MKHAFAHFNFNVTDLDKSVVFYKEALGLEEVRRKETDSFTLVFL
                     KERETSFMLELTYLKDFDKPSYDLGDNEFHLAMISDDFDASYAKHKDMGCICYENKAM
                     GLYFINDPDGYWIEILPKR"
     gene            complement(78072..78797)
                     /locus_tag="PLF14_01050"
     CDS             complement(78072..78797)
                     /locus_tag="PLF14_01050"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT69920.1"
                     /translation="MYITHLIHSTFLIETKKAFILFDYYGKGQLPEHFDKDIYIFSSH
                     SHEDHFDMKVLELFADNPRAFFIMAYDIRRNFGDRIKSEFPNVQDRITYLMPNRETDI
                     GDVHIETLASTDAGVAYVVTHDNKVFFHAGDLHLWIWKEESDRHNERMSKAYRKRIAE
                     LKNRVIDYAFYPVDLRQEEHYDKGLKYFLDNTRTLKVFPMHYFENYSTTDKIFQNKSL
                     SDHYEKIVRIEHINQVFFFEEEA"
     gene            79000..80094
                     /locus_tag="PLF14_01055"
     CDS             79000..80094
                     /locus_tag="PLF14_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HOT69921.1"
                     /translation="MTDKKQNKFWSKVKKYLNHVFIDGLSGMALGLFATLIIGTIIAQ
                     IGSLIGGTAGKYINMVASVAKALTGAGIGFGVAYKFKESILVSVSAGVAGMIGAFAAS
                     ILSGTAFPGAGLLLTGPGEPLGAFLGALAGIEVGHLVSGKTKVDILVTPLVTITVGAA
                     VGLLIGPPISKFMEAVGAVINWGAEQQPFIVGLVVSVVMGCVLTLPISSAALGIILKL
                     SGLAAGAAAAGCCAQMIGFAVSSYRENKVNGLIAQGLGTSMLQMPNIVRKPVIWIPPI
                     VASAVTGPISAWALRMTCDATGSGMGTSGLVGPIMTFKTMLADGFPVWYTILSIIGVQ
                     FILPAVISLFVSELMRKKGIIKLGDMKLDM"
     gene            complement(80242..80556)
                     /gene="trxA"
                     /locus_tag="PLF14_01060"
     CDS             complement(80242..80556)
                     /gene="trxA"
                     /locus_tag="PLF14_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014354906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOT69922.1"
                     /translation="MMATLKITKDNFEKEVLKSGKTVLIDFWAGWCGPCRMIAPTIEA
                     VAAEVTDKVIGKVNVDEEPELAQRYGIMSIPTLVVIKDGNVVRRAVGVQSKQAILKLL
                     DI"
     gene            80709..81386
                     /locus_tag="PLF14_01065"
     CDS             80709..81386
                     /locus_tag="PLF14_01065"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOT69923.1"
                     /translation="MENNNVRRMPLLGDDAPAFTAVTTQGTINFPADYKGKWVILFSH
                     PADFTPVCTTEFMTFGSMIDEFKALNTELIGLSVDSLYAHIAWLRKIQELEWNGKKNI
                     EIKFPLIEDIRMEVSNLYGMVQPGQSNTQAVRAVFIIDPKGKIRTILYYPISTGRNFD
                     ELKRIIQALQTADAESCATPADWRPGDDVIVPTAGSCGVAKERMESKSKDMYCLDWFL
                     CFRKLKK"
     gene            complement(81489..82265)
                     /locus_tag="PLF14_01070"
     CDS             complement(81489..82265)
                     /locus_tag="PLF14_01070"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOT69924.1"
                     /translation="MKPSLVIFGKTVASYRLFAILAVISGTTILFLLLGKKKIKVWKT
                     AILAVFLLVFFFIGARLFNIFVNPAAYEDISVFEPEFVGLSIYGGFFGALLAVVLWCL
                     LFRIPIFGILDLCIVPAAVSFAIARIGCFLNGCCSGKFTNSIFGVVYPKFADAGGLAS
                     YPVLPTQLFEMAFAIIILIPALIVQKKSDKFPEGSAFLVYANLFCIMRLFILPFRRLP
                     YKSWVKHYFYPALYLILIFISLVIFLYRYIKNRKVNEKGY"
     gene            complement(82272..84764)
                     /locus_tag="PLF14_01075"
     CDS             complement(82272..84764)
                     /locus_tag="PLF14_01075"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOT69925.1"
                     /translation="MYCRYCGANNNDDTTFCTNCGQKFNQDLLCPKCNTILKQGTGFC
                     TVCGTPITQVTPQPKKRAKKPVKNLKKKVLISVIAVVLIVSIVLGIMYVPGIIDRNRR
                     YKTKAGRAQKGTVVTVGRESPVVQAEGISVDFEGMTSDEDVELKISPTKGASDEEMGN
                     ALASYRLTADDEESSEFAGIVSIAIPLDMTDYDDLDVCAAYYDDQSSKWRILPHETFK
                     EENKVVIYTDHFTDFALFKISKSDSRFATAALNYTVIRSFFQNGDPLKFYEKLKKGNL
                     PTDSDMVTVGLEFLNFTSQAYEGKVTMQAIMNSIPLTQIEGLTSTLSKIGFGLVLLKA
                     SYEYFILDSGSKAAQTVLTGLMEMSWFAMAGAIGGPVAATVAFTGWAAVNIAGAAISH
                     KRSKKIDLAVKTQEDFINESYLYCIKKGDRYSQSKSGAWYYMYNSPGMNYYAYKAGSD
                     AEWLWLIDKLYQLNKDDPEAFITAVANAINFYPRLWAATPAAYRGNQWRADNDPMRRV
                     EYPSLSEEDERIVTATVRENLRKRMIPIVAQYKKKIFAKMQAEFEETLLKEKEKLNKT
                     ITFEIVSDQPDTFYDKYKGATMRFAVPDGLVTKLSKTQIARFSNQWRFPMIKKGENPK
                     LEATYLGYILSGMPDTLNIFLKTSSLRKNLPDRTVNFTFRMPDTVITVPDLHPTFEEL
                     LGSYPDGTLFIEDVFIGENFEKALARLQEKADEMAKSIGCDSAAEITITVGEELIGET
                     LQSTMTISAHEEENEGSIYFDRVGLDSMNFSYYEDKGRIVLTDLYIRGVFIAAYLPNR
                     EGISVTGNIMMTIPGMKMTDYHVRLRATFVKK"
     gene            84918..85886
                     /locus_tag="PLF14_01080"
     CDS             84918..85886
                     /locus_tag="PLF14_01080"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT69926.1"
                     /translation="MSKYNYSKELKKPISLGNINFENILIRTLIRTGCRLSDLQIKLL
                     KGKEDFSRRTVHIPVGDNIKIPCTVIEPKNQKINLPAIIYLHGGAFVLPLASLMIKNA
                     LYYAEHLQCRVFLPQYRLAPEYPFPIPLYDCYNAFNHLTENADTYLIDKNNILIYGDS
                     AGGCLAASVCHMLRDNKKYMPNAQILIYPVTDNSVTSKSMEQYKNAAWTKTATVHMWN
                     LYLKNGHAGMLGYAAPLHNSDFGNLPPAYIEAAQMDTLRDEALSYADKLKDTKIPVET
                     YLVKGAYHGFDMDHTSPLVKKVLKHRIKVMRNYFAARKISDAFFHC"
     gene            complement(85891..86688)
                     /gene="zupT"
                     /locus_tag="PLF14_01085"
     CDS             complement(85891..86688)
                     /gene="zupT"
                     /locus_tag="PLF14_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HOT69927.1"
                     /translation="MGNVWLAFGLTLIAGLSTGIGSLIAFFAKKTNTKFLSTSLGFSA
                     GVMIYVSMVEIFSNASDVLTEVHGEKIGVLYTIIAFFGGMLFIALIDKLIPSEKNPHE
                     VKMVETEDEEVNKDRLKRTGVLTALAIAIHNFPEGLATFVAALQEPRVAIPIVAAIAI
                     HNIPEGIAVSVPIYYATGSKKKAFLYSFASGLSEPIGALIGYLILMPFMNDTVSGILN
                     AAVAGIMVFISVDELLPSAREYGEHHLSIYGMVAGMAVMAISLWLFI"
     gene            86885..87712
                     /locus_tag="PLF14_01090"
     CDS             86885..87712
                     /locus_tag="PLF14_01090"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT69928.1"
                     /translation="MEFDGLQRKRDYKAVQKTVVIAITVILLLIPFMIWQNNSLTVTE
                     YDKYSSNLPDAFDGYTIVHLSDLHSKDFKGKGTVKINALNPDIIVITGDLIDLHNRKL
                     DVAEDAVKMLTNIAKVYFVTGNHEILSPIYPSLLEIFDEYGVTVLDNEQQKIYIGEQS
                     ITIYGYADCEYGIDTDAALDKEDFNLVLCHRPYAPEVFNKVNADLVLSGHAHGGQIRL
                     PFIGGLIAPDQGFFPKYYQGMHDYLDTSVVISRGLGRSILPLRLFNRPEIVVITLHK"
     gene            complement(87744..88646)
                     /locus_tag="PLF14_01095"
     CDS             complement(87744..88646)
                     /locus_tag="PLF14_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOT69929.1"
                     /translation="MDKKSKFGAHFTALITIMVWGSTFIASRFMLDYFTPFQVMIMRF
                     ILAYIVLLLIKPKFTKIIWKDEPVFFLMGLTGCSLYFIAEYKALTMTSASNVSIIVAS
                     SPILIALLAHIFTKDEKLKVNTVAGFVIAFIGVAFVVFNGTFILKFNPIGDLLAFLSA
                     LSWAAYSLILKKVINRYDNILLIRKVMFYGIITTAPFLLIERKPFDFTAFSDLKLVIS
                     ILFLGVIGSGLCYLIWNIPVSKLGITVTNSYIYAIPFVTMILAATLLKEKITVLGILG
                     AVLIILGVVITSSPKNLLKTRKNK"
     gene            complement(88742..90313)
                     /locus_tag="PLF14_01100"
     CDS             complement(88742..90313)
                     /locus_tag="PLF14_01100"
                     /inference="COORDINATES: protein motif:HMM:NF028272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC exporter domain-containing protein"
                     /protein_id="HOT69930.1"
                     /translation="MKKNAITYLLAIKLKNNIKELFKKPARLIYAVIMIALIVVTAIS
                     GKKRVDENTVFRDPRELTAGLTALYIIMFSLIAYNGFKSGATMFKMPDVNLLFTSPIH
                     PRRVLFYGLFQQLGTSLLLGVFILYQYSWLHILYGINFLQLIIILLGYSLSVFVAQLT
                     AMVIYSFTSYDDKKKRIAAVIFFGIIIAYAIGLVIFLLKDMDNVVTNLVTAGNSILLR
                     LMPGGGWIGGILNGILLKDISAILLGSVLTVAYIAGMTILLSYAKQDYYEDVLKTSEE
                     QFSLASAQKEGKIMETTGKVKVGKTGLSKGEGASAFYYKHKLEERRARRFILEPAGIV
                     FMIVSIIFAVFMREYGIGPIFAYTTYMQMISVKMGRFSKELGKPYIYLVPETSFKKIL
                     YNIAQSFAGFLVEAAILYIALAFILKLTPFDVVVLILARLSFSYLCTACNILIQRVFG
                     TISSRVLIYVFYFLSVAVMAAPGAVVALLIDNTGWILLSDYILPFLAITVCNVLLSLL
                     LIYLCRDVLKYAELK"
     gene            complement(90310..91020)
                     /locus_tag="PLF14_01105"
     CDS             complement(90310..91020)
                     /locus_tag="PLF14_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006906466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT69931.1"
                     /translation="MLEVLNVTKKYGKVVANDDISFSVNGGQIAILLGPNGAGKSTII
                     KCISGLLRFKGEIKLDGYDNKSIEAKRLLGYVPEVPAVYDLLTVEEHLEFIARAYRIK
                     DDEYERSLLERFELWDKKSKLGKELSKGMQQKLSICCALVHKPEVIVFDEPLVGLDPH
                     AIKVIKAIFQELKQDGRTILISTHMIDSVEDYWDVAHIMMDGKFAATKLNESVSGDDK
                     ESLEELFFRITEKKSEEA"
     gene            complement(91253..92275)
                     /locus_tag="PLF14_01110"
     CDS             complement(91253..92275)
                     /locus_tag="PLF14_01110"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT69932.1"
                     /translation="MSKEIIRYGIIGCGGIAHTHAEALLQIKGAKLVGVSGCSPQEPV
                     PFAEKYGIKSFDSNEHLLSSEEIDAVTVCTPSGYHAPIAISAAQHKKHAIIEKPLALT
                     TESAKQVVQAFAKNGMTGSVISQLRFYDGIRKVKSMIEDKRLGDLIMANLTMKFHRSQ
                     EYYDSGKWRGTWELDGGGAMMNQGIHGVDALLYFIGEVKNVYGYIRTLTHKIEVEDNA
                     VAVVEFRNGCLGTIIASTSMVPACNRTIEIHGTKGTILLEEEHIARLYIEGEKEYSAD
                     DIAQGEAASSHIITAGGHVKQLTDFTEALLEKRAPYVSLEDGMKAVDLITSVYASSKT
                     NSIYGR"
     gene            complement(92272..93621)
                     /locus_tag="PLF14_01115"
     CDS             complement(92272..93621)
                     /locus_tag="PLF14_01115"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOT69933.1"
                     /translation="MSELAIFGGKPAIDKTKVPDSLFKWPIVTKEDEDAVLDVLRRGA
                     MSGLDVTEKFEKEFAKWQKRKYAIAYCNGTMSLQAAMYACKIGAGDEIICPSKTYWAS
                     CLQAFNLGATVVFADIHPRTLCIDPDDIERCIGPRTKAIMVVHYLSHPADMDPIMAIA
                     KKHNIYVIEDVSHAQGGMYKGRMLGTFGHVAAMSAMSGKSFATGEMGILVTDDKEMYD
                     RAMAYCHYERNNSKYISTDYLLKYASMPLGGVKGRVNQMCAAMGRVQLKYYDERTAEI
                     RKAMKYFWSLLEGVPGIRAHMVDESDGSNMAGWYAPHGLYCPEELEGLSVERFCEAVR
                     AEGFAARAGGNRALHLHDIFKDYNGLNYDKPARVAFTDRDVRKLDEGITVSQHVQIYS
                     IPWFKHYMPECIEMYANAFKKVALNYKELLAGDEKRGTESGRWFFYTDKKEKSKGAK"
     gene            complement(93706..94632)
                     /locus_tag="PLF14_01120"
     CDS             complement(93706..94632)
                     /locus_tag="PLF14_01120"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOT69934.1"
                     /translation="MTDKSNLKDIPVFRIEDNPDGHLMFYVKKYRPENGTHYLHAHKC
                     IQINYVYSGKAVHIIHNKKYDLVKGDIFVIPPNVPHQIQPAGVSDTEIVEFEFDPKFI
                     NEFLNDLKKGESFWDFAYIEPFLVEEHSVRPRFNLVGKIQCETENIFNEILREYENRE
                     PGFDLIIRSLLLKLLVMVGREFTKKISNSKSAKLFERHNDAIKRAIRYIEEHYTQDLN
                     IDAVAKKFHLSPSFFGYLFKNLTSKTFKEYLTGLRIAKASEMLKHTDDLVINICYESG
                     FNNVNHFNRTFKQIIGMTPGQYRKRFMNGNSQ"
     gene            complement(94632..95315)
                     /locus_tag="PLF14_01125"
     CDS             complement(94632..95315)
                     /locus_tag="PLF14_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012058851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HOT69935.1"
                     /translation="MDNVISYLRELNFVTMIIRIVLAFLCGGLIGMQRERHGRSAGFR
                     THIIVCIGSALTAMTGIFMSDILGFDTDPMRLSAQVLSGIGFLGVGTILVTGHTHVKG
                     LTTAAGLWTIAAVGLAIGAGFYEAAIICVIVLAIVMAYLGNFGSRRIKMDVYLEITGS
                     RNINTVIKDIEKAGYTIDNVEVQSAKSGTYGNIGLSIVIGIPRKVDEEKVINDITAVG
                     NVVLVVRCD"
     gene            complement(95333..97234)
                     /locus_tag="PLF14_01130"
     CDS             complement(95333..97234)
                     /locus_tag="PLF14_01130"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOT69936.1"
                     /translation="MHYGFIKVAAASPSLRVADCKYNCNGIIKTIKKAYDRNVQLLVF
                     PELNITGYTCDDLFSHKLLIDQALTGLLDIVKASESKEMLIVTGLPVKAEGYLYNCAA
                     VVYNGNILGIVPKTHLPSYNEYYEMRIFRSAPKENSVIDINGTSVPFGTRLIFRNMLM
                     NDFSLAIEIGEDLFAPVNPGSLHAMNGAAVIANPSANNELLDKAGYRRTLIEAQSRKS
                     ICAYIFSNASDRESTTDMVFSGHKMIAENGTVLSEALPFTGEYIETEIDLERLSHDRK
                     SRLPVAEQHDSGYKIIPFTMKPVECRISRTIKKHPFIPEDNDTMEKRCDLILNIQATG
                     LKKRMEHTNCKTAVIGISGGMDSTLALLVTHRAFKDMGRSPSDIVCISMPCFGTTKRT
                     KDNASKLSRILNTDFRVINITASVKRHLADIGHDGVTTDAAYENAQARERTQVLMDIS
                     NMTNGMVIGTGSLSEQAIGWSTYNGDHMSMYNVNTSVPKTLVRHIIRYIADRSESKLK
                     AVLYDILDTPISPELIPGVNGEIIQKTESIVGPYELHDFFLYYFLRWGFTPKKILMLA
                     TYAFKDNYSADEIKKWLKIFLRRFFLHQFKRSCSPNGPKIGSVALSPRGDWRMPSDAS
                     SEGWMKNLD"
     gene            complement(97330..98679)
                     /locus_tag="PLF14_01135"
     CDS             complement(97330..98679)
                     /locus_tag="PLF14_01135"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT69937.1"
                     /translation="MSEFLHAIKTKWQNSSFRKTFNLNDEKAKGRDCLLASSILSSLA
                     TQLSGGAFYIGFLICFGMNTVNIGIIIFVPMITRLLAIFSPLILERLRRRKVFLTVLA
                     VLANVFNILGVTLLPDLITSQTGRVVGFTLLVTISTAISAIIDSGYSAWHIPYLPNDV
                     RADYYTLTSGISGFFTQTVVLIVSLIIDSFAGSSEQIEIITTFRYIAFALAVLNIVVL
                     AIPKEYPYEPSRKKTKIKDMLVLPLKNKKFLWTVLIVALYYLGFNLTSAAINGFLLKS
                     VGLSYWEINLINAIYFLIHVFFGKALTKSLNKNSRYRILGYALLLQGPTYIMYAFVTS
                     ENYIWLWVIVRLMQHVLGVIINSVIASLLDDNIPESDRTNHISFYNLAVHMSCFFSMM
                     LGTLCFWRIGNNVVDIFGFKLAGIPIVLAISGIAQILVGLLCRILNDKLLADDLQNG"
     gene            complement(98766..100115)
                     /locus_tag="PLF14_01140"
     CDS             complement(98766..100115)
                     /locus_tag="PLF14_01140"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT69938.1"
                     /translation="MNEYLHAIKIKWRNSAFVRTFNLHEEKAKGRSCFLASSLLLSLV
                     TQLSGGIFYTGFLLSFGINIVNIGIITFVPIITKLLSIFSPFILERFRKRKVFLTAMS
                     ILANVINILGVTLLPDIITSEAGRVIGFTLLVTTSSAISAIIDPGYSAWHAPYLPDDI
                     RADYFTLTSGINSFLAQGIALVLSLVVDSFAGSPGQLEIMTGFRYAALVLAVIRILIL
                     AIPKEYSYERSGDKIKISDIFVLPFRNKKFLWTIIILAFYYMAINLTTASINIFLLQD
                     VGITYTQINLVNAVYFMFFIFFGKMWKKFLKKHSWFKTLAYTMFMQGPTFIMYAFVTP
                     ENYIWLWFLIRLIQHVLGVIVNAVTASMLYVNLPDKDRTNYIAFYSIAVNMSIFLSMM
                     FGTFFLSVMGENTLNFFGFQMAAVPILLAVSGIMQIAVGLLSRKLNDKLLPDELLRR"
     gene            complement(100191..101540)
                     /locus_tag="PLF14_01145"
     CDS             complement(100191..101540)
                     /locus_tag="PLF14_01145"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT69939.1"
                     /translation="MNEYLHAIKIKWRNSAFARTFNLTDEKAKGRSCFLASSILSSLA
                     AQLSGGVFYTGFLLSFGINIVNIGIITFVPTITRLLSMFSPLILERFRSRKVFLTVMG
                     ILANIINILGVTLLPDIITSETGRVIGFTLLVTISSAISAIIDPGYSAWHAPYLPNEI
                     RGDYFTLSSGVNSFVTQGVVLILSLVADSFAGSPRQLEIITGFRYAALVLAIMHILVL
                     AIPKEYPYEQSDSKIKLSNIFILPFKSKKFLWTVLLIGFYYLGASLTNASINIFLLQD
                     VGVTYSQINLINAVYFMFFIFFGKMWKKVLKKYSWFKTLAIAMLLQGPTFIMYAFVTA
                     ENYTWLWFTVRLLQHVLGVVSNTVIASMLYVNLPDTDRTYYVSFYNIVIHMSSFLSMM
                     LGTMFLSWMGDNVLNVFGFKMAGVPVLLAITGIAEVIVGILSWKFNDKLLPDDLSKR"
     gene            101763..102155
                     /locus_tag="PLF14_01150"
     CDS             101763..102155
                     /locus_tag="PLF14_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HOT69940.1"
                     /translation="MKKDQNRFFICRRCGNMIGMIYNSGVPIVCCGVNMEQLVANTTD
                     GANEKHLPVIKQNGNNVVVEVSTVVHPMVKEHYIMWIYLETAKGGQRKSLLPGEKPVA
                     TFAITDDDKVIAAYEYCNLHGLWKTAVK"
     gene            102195..102353
                     /locus_tag="PLF14_01155"
     CDS             102195..102353
                     /locus_tag="PLF14_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOT69941.1"
                     /translation="MKKYVCMVCGYVYDEKEGDPDNGVDPGTKWEDVPEDWTCPICGV
                     SKEEFEEE"
     gene            102507..103568
                     /gene="aroB"
                     /locus_tag="PLF14_01160"
     CDS             102507..103568
                     /gene="aroB"
                     /locus_tag="PLF14_01160"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOT69942.1"
                     /translation="MRKITVAGSKKYDIIIDKNILSEAYQYIKPFLTGNIVYIVTDDI
                     VDSLYGSILTDSLESNGLSVFRYIIPHGEQSKNADNYIAVLNDMASKGVSRSDMLIAF
                     GGGIPGDIGGFAAATYLRGIPLLHIPTTLLATVDSSIGGKTGINLKEGKNLAGAFYQP
                     QAVICDLSLLDTLPDEVFKDGYAEIIKYGILSGNQLFNRIKKPLKSDIESIVYECIKI
                     KRDIVQKDESDIGIRQLLNLGHTIAHAVELLSGYTISHGAAVAKGLHLISKISYTQGL
                     CTKEVLTKITDMLNLHGFNTDLLYGAKDIANAITADKKRSSGHINLILIRDIGKCTIE
                     KTDMSDLTDIIQTATEYRG"
     gene            103570..104832
                     /gene="aroA"
                     /locus_tag="PLF14_01165"
     CDS             103570..104832
                     /gene="aroA"
                     /locus_tag="PLF14_01165"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOT69943.1"
                     /translation="MDISVTVKNPGGTVKAITSKSHAHRHLICSALSDVPNFVEVTDT
                     SSDIDATVQCLNSLGANITAEKNGYYINPIKTVRINALLDCNESASTFRFLLPVTCAL
                     GAKSSFIMKKGLSKRPITPLYNQLIGKGCTLSLEGSVPFLTEGQLKSGIYQLPGDVSS
                     QYISGLLLALPLLKNKSAILLTSDLQSSGYVDMTVDVMNQYNVKIIKTEKGFEIPGNA
                     AYTPTGNMSIEKDWSNAAFFLCAGAFSERAITVTGLNIHSRQTDKTIIDILKTTGAYV
                     TVNKDTVTVKKNRLNATDIDASQIPDLVPVLALLAAVSEGTTNIFNAKRLQLKESNRL
                     SSTCDMLKTLGADITEKPDSLVITGKEKLNGGTVCSHNDHRIAMTAAIASIVCENAVI
                     IKEAHAVNKSYPAFFRDFASIGGITERI"
     gene            104837..105928
                     /gene="aroC"
                     /locus_tag="PLF14_01170"
     CDS             104837..105928
                     /gene="aroC"
                     /locus_tag="PLF14_01170"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005949203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOT69944.1"
                     /translation="MSSVFGKNITVTIFGQSHSKGIGVVIDNLPAGFSIDMEQLTAFM
                     QRRSAIDKPLSTKRKEKDEIEFLSGLVGNTTCGAPICAIIYNKDINPEDYRSFKDIPR
                     PSHADYTAHIKYKGFQDTSGGGHFSGRLTAPLCIAGGICLQILEKYGISIHAHIKKIY
                     DTEDISFNPAEDNRENIEKLLNNDFPVIDKQKGILMQERIRTASQQNDSLGGIIECAV
                     NNLPAGIGESMFDGLENNISKAVFAIPAVKGIEFGNGFDCANLKGSENNDPFYIKDSK
                     ILTRTNNHGGILGGISSGMPLIFRVVVKPAPSIGIEQESVNMTNNNSEQIKVGGRHDA
                     CIVPRAVACVEAAAAIAILDMYASYRIWE"
     gene            105954..107462
                     /locus_tag="PLF14_01175"
     CDS             105954..107462
                     /locus_tag="PLF14_01175"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOT69945.1"
                     /translation="MNLDDLRKEIDRIDDKLLQLFQERMGIVKDIAEYKKAHSIPILN
                     SELEKAKLSNIISKTDEELVSYTQILFNTLFDTSKSYQEKLNISQPAALGYSDKKFGL
                     LGAELQHSYSPLIHSHLGDYEYGLYEKQSDQLYDFVHNSNLDGFNVTIPYKKDIMKYC
                     NILTPIAKRTGSVNTVIGKANNLTGDNTDYYGFRYMIERSGINPEGKKILLLGDGGSA
                     ATIKAAIQDMNAGCLVTVSRSGQENYGNISNHYDSDIIINSTPVGMYPDNGKTVIDIS
                     SFKKCIALFDLIYNPAQTRLMYEASKLGIKAYNGLSMLAAQAKKAAELFTESVIDDSV
                     IERVVEKVAKKTGNIVLIGMPGSGKSTVGKALAELTERAFIDTDEVIAATYNKSASDI
                     IKDKGEVFFRDIETKVLEKVTKQSNCVISTGGGVVTRERNRYLLRQNGIVVFLKRDLS
                     LLCTLDRPLSQQNTLEELYKARKNNYLGWSDYQIDCDDTDKAHLKIYEVLGL"
     gene            107459..107896
                     /gene="aroQ"
                     /locus_tag="PLF14_01180"
     CDS             107459..107896
                     /gene="aroQ"
                     /locus_tag="PLF14_01180"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004839113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOT69946.1"
                     /translation="MKLLVINGPNMNMLGIREPEIYGHATYADLVRYIKDICTQKNIQ
                     CEIFQSNHEGDIIDKIQDAYMISDAIVINPAAYSHTSIAIADAIKSVGLPAVEVHLSD
                     ITKREDFRKHSYTANVCIKTVTGKGFDGYKEAILLLKDAIDRC"
     gene            complement(107985..108809)
                     /locus_tag="PLF14_01185"
     CDS             complement(107985..108809)
                     /locus_tag="PLF14_01185"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOT69947.1"
                     /translation="MNETLSKLWDTLLEASATAGLRILLAIAVLIVGLLLIKYLVKFI
                     VKSKGFNKIDVTVQTFLKSALAITLKVLLIISVIGILGVPLTSIIAVLASAGLAIGLA
                     LQGALSNFAGGIMILVFRPFKVGDFVDNGTHMGYVDSITIFYTRLLTKDNKLVTVPNS
                     TMTTASITNFSAKELRRVDIEYKVSYKNDIDKVKSVLTSVAEKHPLVLKDPSVTSGVY
                     AHGPNSLVYVLRAWCKADDYWTVYFDMNENVKKSFDEQGIEIPLQQVVVDIKDKNK"
     gene            complement(108907..110079)
                     /gene="tyrS"
                     /locus_tag="PLF14_01190"
     CDS             complement(108907..110079)
                     /gene="tyrS"
                     /locus_tag="PLF14_01190"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOT69948.1"
                     /translation="MNNGKTMEILMKNAVNVFNEEELRTKLNSGKSLKIKFGADPSRP
                     DLHLGHTVPLRMLKVLQQAGHHIIFVIGDFTGMIGDPSGRSKTRSQLSYEETGKNGQT
                     YFEQVTKILDPSKTEIVYNSEWLGKMRFADVIELAGKYTLARIMEREDFSARYSNNQP
                     IGIHELLYPLIQGYDSVAVNADIEIGGTDQTFNLLVGRELQKDYGQIPQVVMTFPLIA
                     GLDGVEKMSKSLDNYIGIDESAEIMYEKCMRIPDGLLTDYFRLTTDICEAEYTDIISE
                     DIREAHKVYARKIVEMYHGRDCINAAEDRYNSIAAKAIPDNIDVFHIEEYETGIIDVL
                     VKTGLATSNSEARRLIAGRGIKIDGITVNDFSVRLKGKFVICRGKNKFIEVRMKMI"
     gene            complement(110337..111110)
                     /locus_tag="PLF14_01195"
     CDS             complement(110337..111110)
                     /locus_tag="PLF14_01195"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT69949.1"
                     /translation="MESSIKKFWNDGSDEYYNRAYNINNNAIRIKADPWWAFPVPVRN
                     MIQNAFGSMKGIKVLVPSSGDNGAVFAFHLLGATVVSADISEQQLGNAKKIADNERWN
                     IEFIQADTMKLDGMCDSEFDLVYTSNGTHIWINDLQAMYSNFNRVLKPKGKYIMFETH
                     PFNRPFDDSGAKIKVKKPYNNTTGNHWRIMDIFNAVISQGFYILHLEEFHAEPGAHDL
                     WFYKTIKEAEADGNRKYDIKHNPFAALPAWFGLSSIKRY"
     gene            complement(111094..111636)
                     /locus_tag="PLF14_01200"
     CDS             complement(111094..111636)
                     /locus_tag="PLF14_01200"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOT69950.1"
                     /translation="MLKLQGKSIYLATLERADCRKLFEDNEYDFENPTDILYIGYSVE
                     GADKWFEEIQAAQGKTHVRLGIFLNDGTVIGDIALQNIDNRNRSCSIGMGIQKLENRN
                     RGYGKQAVSLILEYGFCNLGLERITAGTLDCNISAKKSLEKLGFVLEGRERKAIYFGG
                     VRHDRLCYAMLAEEYNGKQH"
     gene            complement(111698..113416)
                     /locus_tag="PLF14_01205"
     CDS             complement(111698..113416)
                     /locus_tag="PLF14_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT69951.1"
                     /translation="MVKKKKTGLVITVAVIVLAVVATLLFLFRDRLFCNIGHFNVTTF
                     NSDIVIKRSDAQEPLNMPYRYSKALLDKRLVFREEIERLNITTVRYEISKTGLTLYNC
                     KEVLKNPESGETKKVIESIKYCKGITALSGLTADKADSKITIYQGYSADLLEQSLHNY
                     VIIPSTLSEHIDSQLSDNEKVLFLINSGTSGLAYFTIIGEYETKHRHDTLYFSYSGLS
                     NVVLGGKEDIVGHIDYMGIDVNDKANLVKFSYFLSEYFADYNVLSQYEKRINKFNEPY
                     QYMYVNNVDILPINLSEDSGFEKNIITVTGIDGNDNLQMSHVYGDALIEDYHKYSQYI
                     TDIIISTGVKGEDWSKYPLNVKIPCYGINFGGYGLEGFYVKYTEYYQSHGMDSPWYHQ
                     AVTSVREIKSMKKNCDITFYTNYTENDLVVIRKEDYVEPKDHLDSGITGYAIVPKMIW
                     ESVRNHPDIDYQIIRLFEQPKKEDNPSGRMRFGFKVIGYYETADESDTVYVTYTGYNR
                     KYVKEPFKNECILSIVIETRSDADITPLLEYLEQYFAPASDTSKYAGKKNLLGMEYEY
                     CYTINE"
     gene            complement(113497..115212)
                     /locus_tag="PLF14_01210"
     CDS             complement(113497..115212)
                     /locus_tag="PLF14_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT69952.1"
                     /translation="MERKKKAIVVIVIVIVIAVIAAAMLYIFRDSLFQKEDNNVVSSF
                     NSDIVIKRMDTGESLNMSYKYAKSILDKRRTFIEEIANINISSVRYKMEENNIKWYTN
                     EGFLVKDDTDKDREIIDAIKYCKGISALSGILSDREDCKIMLYEGYSEELLLKGYENC
                     AIIPSSMSKYINKEIPDNEKVLFISDTYFGNTFYFTIIGEYKTKSEYDTLYVSYAGLT
                     ELIRARRTDIPNHVDSLELDVYENKDLTGLVNYLSQYFAEGSVYSEYEGRFNVYNEPY
                     EFMYVHSLNIEPVVPLQDIIYANYEIIISRIDGKSDLEMSHVYSDALIEDYDKYSQHI
                     SDIVISTGVKGVNPDDYPTDSSEPGYYNYPLYSIQMNFGFQSQFWNNYEDFPPFYQAV
                     TGISEIKSMKKNCKVTLHFGYSSKDMIVPKQTDIDHYVKGYAVIPLPMHEANRNRFDN
                     VNIIVRMNEAMAEYEESGRRIFSCRTISCFKVIGYYETTDKYDVIYITYAGSNEKYKL
                     EPFENEHIESVTLWAQDDTDIKVLQGYLEQYFAPATDTSKYAGKKNALGRDYEYCYTI
                     KSNAD"
     gene            complement(115295..117097)
                     /locus_tag="PLF14_01215"
     CDS             complement(115295..117097)
                     /locus_tag="PLF14_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT69953.1"
                     /translation="MKKTNMIIAVALTVIITALLLASCDGLSKKEEGLIDATFSSDIV
                     IKRVDGKQPANIPYKYTKALLSDNIAYIEGVQAVNISSRRYNMKDNDISMRGEKDLFV
                     SSEEDKVAEVMGSIKYCKGITSLCTIVGDKGKSKVEFYAGYSEEHLLSVDKTYVIIPS
                     SLGELINEEIPDSDKRIVLEKGAFGIAYCTIIGEYEVKDRSKNKGEPDTLYLSYAGFG
                     NLINIGEEIDISFVDCLEIDADEQADLAKLTYCLTTYFADANTLSQYEGRINMFNEHY
                     SYMYENSVNMKSLDLSPDTDCEKKIITVSRIDGKGDLDMSYLYADALVRDYDKYSQYI
                     TDINISTGLGGHDPYENAKLESIDKGGGFIQTNFILDNADGLNISFASSSTGFRDAVT
                     NMITQNRKDALADVGKGKAWKKHVQYLLHQPITSISEIERMKSNSKVTFYTNYTNRDL
                     VVQRKEDYIESTFKKDGDIKGYAIIPKTMHDAASNLPDLKTHILYLSEKNDAVESNQK
                     VLVGFKVIGYYETDDRYDVVYITYAGSNDKYTKERFRNDYINSVTIETKKDTNITPLL
                     EYLEQYFAPATDTSKYAGKKNALGRDYEYCYTVR"
     gene            complement(117161..118885)
                     /locus_tag="PLF14_01220"
     CDS             complement(117161..118885)
                     /locus_tag="PLF14_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT69954.1"
                     /translation="MENRKKRFAILIIAAVIIVIAASLLFLFRDRLFKKDNFVVTTFN
                     SDIVIKRTDANESLDMPYRYTKALMDNLFIFRQEIAGINIASVKYNMSENYIDWHTPE
                     GVLTDTDRGKGKQVIEAVKYFKGISTLSSIVADKEDCKITIYEGYSEDLLMHDYQNFA
                     IIPSSMSKYFNKDLPADGKVLNIRNMRYGSMLHFTIIGEYKTEEEYDTLYVTYTGLST
                     LIRAGRTDILNHVDCLEIDVNEDKDLNKLMRFLSEYYADAQVLSQYTERNNIYNDPYQ
                     YMFVHSMDIEPIELKENVIYEKSIITISRMDGKEDLEMSHVYADALIKGYNKYSQCIT
                     DLDISTGVKGINPADYPLGSEAFWNQPVYQLLLKYDTVYEAKLKETLGVFPCYHQAVT
                     SINEILRMKKDCKVTYYLNYMNSDLIVPRQKDLLGKIKGYAIVPKPLHEATSDLPNFN
                     NHIVEVYESRVYVGIGGVDPSQIDRSPHFRAQFKIIGYYETTDPYDTVFVTYVGCNEK
                     YKSGAFKNEHIESITMKTKGDVEISPLINFLKLYFAPSENVAEYAGSTNELGLAYEYS
                     FTMKEIAE"
     gene            119217..120857
                     /gene="gpmI"
                     /locus_tag="PLF14_01225"
     CDS             119217..120857
                     /gene="gpmI"
                     /locus_tag="PLF14_01225"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOT69955.1"
                     /translation="MLKKSSEFKEIQGPLLLIVMDGVGLSDKKEGNAVAAANAPTLRM
                     LMDKYPHTTIKAHGVAVGLPGDEDMGNSEVGHNALGSGQIYSQGAKLVFEAIEKKTIF
                     SGKTWLKLKDNCIKHNSTIHFIGLFSDGNVHSNIAHLKAMLEQAKAEGVKKARVHILL
                     DGRDVGETSALEYVEPFEEYLDTLRCNSFDVEIASGGGRMNITMDRYEADWDMVRKGW
                     EIHVLGKGRAFSSAKEAIETYRAETDVVDQFLPGFVITKDNKPVGTIEDNDSVIFYNF
                     RGDRAIEISRAFEEKNFDKFDRVRVPNVIYAGMLEYDGDLHIPKNYLVSPPDIKNTLG
                     ENLSKKGVKQFAISETQKFGHVTYFWNGNNSAKFEGETYVEIPSDKIPFEQRPWMQCA
                     LITDKLLEALKSGEYKFLRMNFPNGDMVGHTGSFDAAVIGVESVDLCLARMLPVVDRL
                     NGVAIITADHGNAEEMYETKKGIISIDRKTGKPVPKTSHTLNKVPFIIYDNRKDKGYT
                     LKEGDFGLANVAATVVNLLGYVAPETWEESMIEEVYNG"
     gene            120850..121389
                     /locus_tag="PLF14_01230"
     CDS             120850..121389
                     /locus_tag="PLF14_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOT69956.1"
                     /translation="MDNFYELASKRQSCRNYSDRPVEKEKLMQCIKSAAIAPSACNGQ
                     PWHYTLITESETVAKLVRHLQSAGANRFTSKTPAFAVITEKPTNAMASMGERLKDQDF
                     SSMDIGISVAHFVLQAEALGLSTCIIGWFNENGIKELLNIKKSDRIRLVIAVGYKDDS
                     DKLRPKKRKSLEDILTVIE"
     gene            121500..122264
                     /locus_tag="PLF14_01235"
     CDS             121500..122264
                     /locus_tag="PLF14_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOT69957.1"
                     /translation="MKYYENMVEMGCFSRSELAKALQLGDNTAATILQQYQKKGYIER
                     VRHNFYVVISIENKQPILSRYGIGSRLFPDASVSHHSAFEVYGYANQVFYEVYVMTSS
                     RFKDFEYDGITFRRVSPKGTVQTEIIGGVRVTGVEQTVIDSINDVDKIAGLEELLRCL
                     DLIPTLNEEKLLFTLNEYQNGFLYQKTGFILKHFRESYGLSDAFFRICADRIPKSDRY
                     LTKEHIGYVYHPEWRLMAPKDINTVINKGVDYHAAL"
     gene            122251..122415
                     /locus_tag="PLF14_01240"
     CDS             122251..122415
                     /locus_tag="PLF14_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT69958.1"
                     /translation="MLHFSKAELNRQANALGFVRDTFEKICRLADVLGFMENNKKPQK
                     VFKIGIAFTI"
     gene            122526..123911
                     /locus_tag="PLF14_01245"
     CDS             122526..123911
                     /locus_tag="PLF14_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT69959.1"
                     /translation="MTDNNIKDFRQDIIKQITKARVAKGLTQSQLAELLGMHRSAISR
                     LESGSHSPSLDLLLQVASVLELKIELTTPSTKVLSHMENNYELRLFDKVLLTFSLEKR
                     GIEGLTAHIISIDKEQLHLLPLGLELSDKGILRWLEKRVIPKNRTFVKEILKTLNLSQ
                     GDTKGIIDICKGLSLNDSFWVVPEGFEGSFAQYNLYENRFSEILSLVAYTGIEQSHGG
                     FTTSPELTTAGMLPKAWRLIDNDGIYLYKGGTFGAANTGNEPYSEYYACQIAQTMGLN
                     AVQYELENWKGILASKCKLFCDIDTAFVPVGRIVTSGGLKAVLKYYSSIGEDFLQAVH
                     SMLVFDAVIYNEDRHFGNFGVLRDNHSGALIAPAPLFDHGLSLFNYAMPKDIKNLDQY
                     AKTRLPAYPDVTFEDICTEVAGKAQAQQLRRLIGFTFKRHPSVNWPEERLTAIEKHLQ
                     KRVRQLLGMVK"
     gene            complement(123919..124164)
                     /locus_tag="PLF14_01250"
     CDS             complement(123919..124164)
                     /locus_tag="PLF14_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009139950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HOT69960.1"
                     /translation="MYRYKTKGTCSTEITFDIQEGKLKDVKFTDGCSGNLQGISKLLE
                     GMEAKKAVSLLKGIKCGYKETSCPDQLSIAISKVLEH"
     gene            complement(124171..124749)
                     /gene="purN"
                     /locus_tag="PLF14_01255"
     CDS             complement(124171..124749)
                     /gene="purN"
                     /locus_tag="PLF14_01255"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOT69961.1"
                     /translation="MNICVFASGGGSNMQAVIDACKNRKINAKVVLVISNNSDAYALE
                     RAKKEKIDNYHVSEYKYKEGFIDKLLELLAKYEIDLVLLAGYMKKLPKEIVERYKVLN
                     IHPALLPKYGGRGMYGERVHKAVLEAKEKESGCTVHVVDEHYDNGKILNQIKVPVLED
                     DTVETLAKRVLEQEHIIYVDTVKKILTKEIEI"
     gene            complement(124773..127298)
                     /locus_tag="PLF14_01260"
     CDS             complement(124773..127298)
                     /locus_tag="PLF14_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT69962.1"
                     /translation="MKRAILSLITIILLLSMCINAFAYCESFERTGKHINAAYVSDVH
                     TPQGHQCGYICQDCDLITVTGYTTLSTCCRCTGNHNYYTLSVGFHTAHGHLVKLKCST
                     CGYTTSYYKKDDSCCDCVGHIRGLTETSREHTQGLGHYYTVPCKECGKVIESGYTTLA
                     SCAICNCSHSYTTSISDYHTSGSGHYYTRKCSKCGYVCDSGYTEKSDCCECRGYHNYS
                     YSVSSAHTTYGHYVTQRCFDCGHNADYYTTVPTCCYCRGYHNYSYTVSQEHTADGHKK
                     TGTCSACGHVTVSYTTLADCCLCRGYHDFSEWSAVSKIHEEQGHRQSRTCKICKQTED
                     RFTTYRPCCLCHGHDMQKTSNIGEHTDFGHAVIYRCITCGYEETIYEDLFGCSLCSNT
                     PTNQEEFILWIQYLGIPVKSVNGNYTASFTMYRDYGYIVYGNPQDVVNNHCVAGEYQF
                     LGYTFDEDPFTNISYQNTEMGPVNANQWIYAEISGAVESWDRLEPSVQKPYMLHTNLI
                     GHGAVNFTANDIGMTKTRVQNGASWLSTGSIYTFKTNGYYATFKVPSMGVPNVNLNAA
                     FTEERLTYSAADVHKKGYINVNFTIDKPLKEIDYIKLSVQGGNSVILQPHERNGQLEL
                     DIPIQSVMPARYKVRVLIEVKSIFNDVHITREYCSVDLYKEETPYNPTDPVQPEEIIS
                     DLPEDIPYQANYDVHITDISLTGYWNIWHLNERFTSLERVRLNVAVAGRVTKLSVLFS
                     EELKNRTYRDRYGNTYDYKDLLGSYDMCPNDICYEGLWENGYTLNTEYILPLCPDTVD
                     SSGNRIKDKYFIKIIAENENKEHTKEYLIDITGNIYDFLYVQP"
     gene            complement(127350..128522)
                     /locus_tag="PLF14_01265"
     CDS             complement(127350..128522)
                     /locus_tag="PLF14_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOT69963.1"
                     /translation="MKKITSLLLIICLIPAAGVCGVNAQATGSRFSDVQSSQWFFEPV
                     IQLYGMNIINGYPDNTFKPDGNVKADEFIKMIIKALGFDIGNAEGYWAQNYIDKAGDI
                     KLLEGFDGEYQKEINRGQAALLISNAMKYLKEPEFYSEKIYPFDLGAYTPDKYKEHIK
                     KCYSAGVINGFEDFTFRFNETLTRAQACTLLIKLIDKTKRDTSVKFDCSFLDNTTKDN
                     VKYKGVTYEELSDVIKSTLTQVKDDIGYIVVEKRVYETKNTYEIRYHKSAINTNPTYC
                     LFAFRFFEGPSGYTETQWGYDTMFLKLELKKFNWDDGIPDSFYKSKVKNALCAALSEH
                     NPYDIADYVIDRYVFLRALKPWDAYNLLEDNSNETLRYVFFTMHNVIGNFTFSKKQ"
     gene            complement(128677..129246)
                     /locus_tag="PLF14_01270"
     CDS             complement(128677..129246)
                     /locus_tag="PLF14_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOT69964.1"
                     /translation="MKPDDFLQWLHVIEKMKCNFRHSKTSCGREESVAEHSYRLILMA
                     YVLKDTIPDVNMDRVVEMCIVHDIGEAITGDIPSFRKTKKDDKKEMEQVLEFIRQLPE
                     ERYQHIKELFSEMEQKKTKEAIVFKALDKMEAVIQHNEADISTWLDIEYELQKTYGIE
                     EAQVCEFLKALREKVLKDTEEKIEKSKRE"
     gene            complement(129246..130028)
                     /locus_tag="PLF14_01275"
     CDS             complement(129246..130028)
                     /locus_tag="PLF14_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT69965.1"
                     /translation="MKNKVFKTLTLVLLISLFCTTLTGCKIQLSEDEYTVGEIPKGSY
                     RLVYYIVNEEQNIDGDLTFKYVTDGNITKMQSYAEFDMWQYRAQVIFDTYTLIPSSSY
                     KSNVFYSGKEKDWSVKTDYTKEKATIKAIQEDKQADKEVAKPARYLDNEMINLTLGLI
                     QIEQDDKVYINVLVNESAMFAPYGVTNQTMETITVNGKSYECVKYSAKYAGFSLFTAR
                     ESYFWYTNDRDRRLVKFVSGSQIMTLKEIIEVPADDPWLFGE"
     gene            complement(130030..130296)
                     /locus_tag="PLF14_01280"
     CDS             complement(130030..130296)
                     /locus_tag="PLF14_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT69966.1"
                     /translation="MLIYGKLIKNNLILVEEKAECVNPDMNFQQRLTNCLVDICKKMD
                     IQVPVWMSKNTKELARFNKTFFTDEQFMEKVYFDRFEIEMKRKD"
     gene            complement(130415..131380)
                     /locus_tag="PLF14_01285"
     CDS             complement(130415..131380)
                     /locus_tag="PLF14_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT69967.1"
                     /translation="MKNTIDITEKVMINGIQQKIHIIGTDSVNPVVLFIHGGPGVVDR
                     HNVMKYMLDITDKVTIAGWDQRGTGGSYKGAKKEDMNLDTFVEDAREITEYLCKKFNK
                     DKIYIQGHSWGSCVGTMLVYRYPENIKAYFGQGQLVNGIRNEEKSYDYVLEKAKAAGN
                     EKDVQKLLEIKRPVEGLYAGDPIKGLMEQRKLLSKYGGATFKCSSMWKSTVKPILFSK
                     EYTLCDIAGVLKGYSFSLKNMWEDIVKLDFSQEYTKFSVPMFYLIGASDYNTPFELSY
                     EYYKTVEAPLKDYIWFNRSSHSPLAEEPEKFSKTLAEKILQVEAM"
     gene            complement(131364..133103)
                     /locus_tag="PLF14_01290"
     CDS             complement(131364..133103)
                     /locus_tag="PLF14_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT69968.1"
                     /translation="MEDFRYIDRGKPIPREQYTDQPYVVVADDGAWVCVLTTAYGQEG
                     AAGTHVGITVSYDRGNTWTDLYFPEGDDLRESAYGVLLKTSFNRIYCFYNFNKNNLRN
                     LICRKERLSHRVDMQGAFVFRYSDDSGKTWSERYEVPIRETKIDRENPYGGKVRMFWT
                     VGKPFILDNDGYVIIHKIGDMLTVSEGWLLRAVNMNIEKDPNKLVWETLPDGDIGLIT
                     PKGGGLIAEEQSMVVLSDKSIYCVYRSIDGHPVETYSRDKGHTWEQPQYKRYADNRLM
                     KHPRAANFVWKCKNGKYLYWYHNNGSKWYENRNPAFLCSGIEYDTKEGRRIKWSQPEV
                     VLYHEDPGVRMSYPDFIEDGGKYYITETQKLDARVHEIPEKFLQSLWNQFESSDITDE
                     GLLSESEGKSVIDMPELPVFCITDRKSHIFATKSTESGVTFEFIYKAGTGKRKILDSR
                     DASGKGVLIEQISDYRINILMNDGINTCCRSSDKYMLKPDQINHIAIIIDGGPDIVSY
                     VINGMYNDGGQDRIFGFGRFSTYFKDMNGKTKARVCKNVLKLRIYGRALLTSEIISNG
                     RGYKKGGKYEKYD"
     gene            complement(133161..133841)
                     /locus_tag="PLF14_01295"
     CDS             complement(133161..133841)
                     /locus_tag="PLF14_01295"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HOT69969.1"
                     /translation="MRSKRKKKKKNRAQKAIAIILLISALLFTSLQLYVMLYPLSDIF
                     DSVEQLEKIERPQAVLVLGAAVYASGNPSPVLKDRLDYAYQVYEYYDGKIAVIVSGDN
                     GTVEYNETKVMYDYLISKGVNADDIYRDYAGFNTYDSIYRAGYIFKVQSVIICTQRFH
                     IYRALYIAKRLGFKAYGYAAENKSMYNMTYNHIRESLARIKAVMETDIMKRQPKYLGD
                     PIPVKQYE"
     gene            complement(133831..134700)
                     /locus_tag="PLF14_01300"
     CDS             complement(133831..134700)
                     /locus_tag="PLF14_01300"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOT69970.1"
                     /translation="MKKCDILVPIFTPFKKDQTVDYEALKKLVRYVLDKGADGLYTTG
                     SSAETFLLTEEERMKTLEVAAKEANGATVVAHVGTPGTALAVMYAKHAKSMGVDAVAS
                     VPPFYYSHGHKAIKKYYTDIADACGLKIMIYNIPGATGVDMSVDQINDLLSDDRIYSI
                     KYTAADYYVLNRIAVASKKPIYSGKDEAFASALMAGATGAIGTTMNIYPQAFVQIKAA
                     FDKKDIASAQKLQTKLNNIIQPMIESGATIQAMKYVSKLAGIDCGEARKPFKNLTAED
                     KKKLEQAVRDNAF"
     gene            134820..135929
                     /locus_tag="PLF14_01305"
     CDS             134820..135929
                     /locus_tag="PLF14_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017250456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOT69971.1"
                     /translation="MKDPRNEKLADLLINYSCRLKEGEKILISVSETDNDLVKRLVEK
                     AYQAKALPFVQISDSSLSPKLYEHITEEQLEIMNERDLNFMKKMDAYIAIRASKNIYE
                     ISTVPSEKLSLASKILKPSFDRRVKYTKWCVLRYPNESMAQLAGMNTEKFTDFYYDVC
                     TLDYQKMSDAMDNLVELMNKTDKVHIKGEGTDLTFSIKDIPAIKCAGEMNIPDGEVFT
                     APVKDSVNGYVTFNTPSVSEGKTFENIRLEFEKGKIVKATANYTEHLNKILDTDEGAR
                     YVGEFAIGVNPYILDPMKDTLFDEKICGSFHFTPGACYDEAPNGNDSAIHWDLVCIQT
                     EQYGGGEIKFDDRLIRKNGIFTVEELHCLNPENLK"
     gene            135937..138138
                     /gene="csaB"
                     /locus_tag="PLF14_01310"
     CDS             135937..138138
                     /gene="csaB"
                     /locus_tag="PLF14_01310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046919 - pyruvyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase CsaB"
                     /protein_id="HOT69972.1"
                     /translation="MKIMHLAGGGDIGGAKTHIISLAKELSRTNTLKLVSFRYGDFAE
                     SAKAEGIDVTEIKSAFLLKDIHALFVEYDKFKPDIVHCHGAKANMMGLFLKRHRNACV
                     TTTIHSDYRKDYMHNLFKQATIGLFNTLALRIIKFHIAVTEQFARTLISRGFEAYNMF
                     SIYNGIDFEKEMPKISRKEYFEQYGIPYNPDELIIGIAARLTKVKDIPTLIKGFAKAH
                     ADNSDMRLLIGGDGEEKQKLTDLANQLGVSEKVHFLGWIDDVDSFFSVIDIDVISSLS
                     ETFPYSLTEGIRAGAIPVSSNVGGASSLIDQKVTGYIFEPKDYETLGNYLYQLSKDKN
                     KRITMQKASYEHTKANFSLKAMKNKQEDIYSLILKHFNEKERNKVVICGAYGRGNAGD
                     EAIKYTTLSSMRNIDPDMDICVMTLRPKKERAIDRVQTIYTFNVIKLFFLLRKSKIFV
                     SGGGSLIQDVTSSRSLFFYTFTLYLAKKLGCKVIMFGCGIGPVSRPFNRKLSGKIIDN
                     YVDVITVRDHMSEKELQSLGVTKPQITVSADPAINLTADKSNTHKIFISEDIPADGKY
                     ICLALRSWKNFNRNEAVAKACEHMYNKYGFTTIFFPMEYPKDLSVSEKTAELLDTPYY
                     LIRNKYPTEQVVAFMSKMEQVVAMRLHALIFAAANYVPVVGISYDVKVSGFMNYIESS
                     ACLELSDVTEQSVINAMEESFKHRERVIKATNTLKEKEAVNKQQFIKLLEN"
     gene            complement(138150..138857)
                     /locus_tag="PLF14_01315"
     CDS             complement(138150..138857)
                     /locus_tag="PLF14_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT69973.1"
                     /translation="MSKGEEMEKILEVKNAVYEYRNKYQTVKAVNDFSFEFERGKLYA
                     VVGKSGCGKTTLLAMLAGLDVPKEGSVVYKGKDTKEMNLDKYRRDDVAVIYQDYNLFP
                     LLTAIENVMYPLHLKGIKDKEAKVTAAKMMDSVGLDSAFHKRLPSMLSGGEQQRVAIA
                     RALAAKADVILADEPTGNLDSENGKNIIGILKKLAHEEGYCVIVVTHDSEISADADEV
                     LSMRDGKLVTKQEDKVN"
BASE COUNT        38673 a        30740 c        25553 g        43910 t
ORIGIN      
        1 ccaacaacta ccgctattaa tagtgtcacc aaaacaagca acactatacc tcttctgtaa
       61 aatcttttca ttatatgcct ccttaaaaac atgaaacttc tttcaaataa tactcaattc
      121 tgaattccga catacacaat gcagaatgat taatgtgctc attagtcgtt ttcatcatag
      181 gtttcttact gtgagcataa acagaatata ttgcctgtat atctgtcggt attccattta
      241 tgcttactta attataattt tccgaactta tatgcaatga aaacattaat agtagcagtt
      301 attactgtta tcatcgcaaa aagtctctta ccatatataa caactcaaag agacaaaacg
      361 gggacaaaaa ttttaaactt ttttcaaata tttcaaattt tcttaaattt actcaaaagc
      421 cactattctt cggctacact ttcagccatt cgtatcacat cttcatagga aacggaagtt
      481 gtacagtcta ttataaacag atatttaccg ttatcccata taacggtctg catatttctc
      541 ttggttgagt aaatagcggt atattgtccc actttcacat atttatacgg tgcatcttcg
      601 gtatctaatg tcatgcttgt gttatgaaga acggactgct taaatattat atcttcgcct
      661 ttttcattag tccaaataaa cttgcgacgg aaatccattt catccttgga ggagagagta
      721 taaccttcag gcagtgcagt taccatatac ttctgttcaa ttgttttagg gacatcaagg
      781 cctctaacat cgaaggaaaa agctgtgtat ttttcatata ccgtaatgat gtaatcgacc
      841 acagcctctc tggctgcttt tacagtcata gtaaaaccga acagcattat tattaccatt
      901 gcggcaacag caactttttt taacggggta ttaattaaag gccaatagct tttcctgctg
      961 tttttaatca gtctgtttat ctttctttca aacctcggag acggagtcca gtctatatcc
     1021 tcatctttag gtattccgga caactttatc cttttagcct ctataaatgc atcacgcaat
     1081 gtgtattttg tttcagtcat tttccatacc ttcttttctc agatagtata taagcatttt
     1141 cttgcctctt actatctgct ttctaactgt ttcctgcttt cttgataatg ccgatgctat
     1201 ttcccgggca gtaaggtttt ccacataatg caagtaaagc acatcgctgt aaatatcggg
     1261 tagactttgt atgatatcga cgatacggtt atattcttct ttagatacga attcctccgc
     1321 aatatcacta tcactgacaa acaaccagga atcatcatca aaatcaaggt ttattatttt
     1381 tctgctctcc cctttcttct tttttaataa attaagggca gtattttccg taatcgtcat
     1441 gacataatag tatgcttttt tggaatgaac aacatcaaca ttctttatat tgcgggctaa
     1501 agaaagcaag gtttcctgta ctgcatcttc cgcatcacat tcattatgca gaatctcatt
     1561 agcgacaaaa aacattttct tacgatacag attatacaat gcaccaaatt tttctttacc
     1621 ctcatcgtca tctatcaggc ttaagaatat caacattgct gctccttttt ccgaaatctc
     1681 ttttggtaag ataatatcaa actatacaac atattagcac acatccgttt tagtgacaat
     1741 aatcgcatag tcggaataaa gggtgttgca tgtacgctaa tgcttacaat attatgagca
     1801 tactgctctt atactatata taacaatcct gaaaggcaaa acggggacaa aaattctgaa
     1861 ctttttttaa attttcttat tattttgcca aattcttaag aatttcctct tttgcatcgt
     1921 ctaccgtgta tatacggtcg tttatattcg ggtgcttttc ctttattttc ttcatcacct
     1981 ttgttatttc cggtatgtct aaacctaaag acaggagttt ctcttcatta ccgaatactt
     2041 catctcttgt gccgtccata actactttac cttgatccag tacaattatt ctttcggcat
     2101 atagtgcaac aagttccatg ctgtgagcta ccagtataac ggtgcactct ttgcttttgt
     2161 gcagattctg tatagtggca aacaactctc ttgttccttc gggatccagt cccgctgccg
     2221 gttcgtcaag aaccagtatc tcgggtttta atacaagaat tcccgcaatg gccgcacgct
     2281 ttttctgtcc tcccgaaagg tcgtacggag agactttaag gttttcttct gttaagcccg
     2341 caagttttgc ggcttttggt actcttttct ttctttcttc ttccggaaca tttcttttta
     2401 tcagtccgaa ctctatatcc ttgtaaacgg tctcttcaaa cagctgatat tgtgcatact
     2461 ggaataccag accgactttt cttcttaagt ctccctgagc tttcttatcc tttgtatcta
     2521 ttccgtctac cgtaacctca cctttgtcgg gttttaaaag gccgttcaga tgttgtataa
     2581 gggtggattt accgctgccg gtagctccta ttatggctgt atattcattg ccgtagaagt
     2641 ctaaattaat atcctccaaa gccttctttt caaaaggagt gttctttgca taggtaaaag
     2701 aaacattctt tacggatatt atcttttttc tttcttgggc tttcattata gcaaccttcc
     2761 tattatatcc gaagctcggt ttcggtctat tgtaagatct tctatgttta tcttttcttt
     2821 tttcaactca tacaacagtt tggtcgcctg cggtacatcc aatcccgctt tgagcatatt
     2881 atccacatcg gtgaatacct cttcaggaga accttttaat gttacggttc cttgatgcag
     2941 cacatacact tcatctgcaa acctggcttc atccatgtta tgagtgatat ttattatact
     3001 tattccttct tctttattga gtttatccac aacacttaac agttcggcct gtcctttggg
     3061 atccaacatg gaagtggatt catcaagtat tatacatgcc ggtctcatgg ccagcactcc
     3121 cgctatggca accctctgtt tctgtccgcc cgacaaggta tgaaccgctc tttctcttaa
     3181 gtcatatata ccaatctgat gcagggcatc atctacccgt tgtataatct tttgcctttc
     3241 atatcctaag ttttccggac cgaatgcaac atcttccagc actcttgtgc ccactatctg
     3301 gttgtccggg tcctgaaata tcatgcctac ctttttcctt atgggcagtt gattttcttt
     3361 ttctttggta ttcataccca gtacgattat atctccgcta tcgggaatga taagtgcatt
     3421 aagcattctg gcaagtgtgg acttgccgga accgtttctt cctaaaattg ctatgtatct
     3481 tcctttagct atagacaagg aaataccgga aacggccttt gttgtttccg gttgtgtctt
     3541 gtatgagtaa tgtgcattct ttacttcaat catccttaat attaaacaag ccttccttga
     3601 aggcttattt tctacttaaa ttatctcaag taacaccatc tctgcggcat caccgcgtct
     3661 ctttcccaac ttatatatct tgacatatcc gccctgtctg tctttgagcc tgggtgcaat
     3721 ttcatcaaaa agcttgttga ccacgttaac tccgtctact ttgttaaccc atttgaatat
     3781 ctgtcttctg gcatgtagtc ttgagggact gtccacggta accatttctt ctaccgtttc
     3841 tctctcaata acttcgtaat ccaccttgta tttgctttcc ttggtcatgg taagccttct
     3901 gcctttatgt gtcttgggac gagtgacttt ctgaggcttg gatgtaaaat tatcgcactc
     3961 tttaacagct ttagctatta tgctgtcggc tatcttcttt acttcctttc ctcttgcttc
     4021 ggttgtttca atttttcctg tcattatcaa acccgtaacc agattcttaa gaactgctat
     4081 tctctggtct gtaggtaagt caagttttct atgtgacggc attttattcc tccttattca
     4141 tccgacttgg caaatttata tcccaattcg tcaagcttgt tatgtatttc ctcaaatgat
     4201 ttcttgccga gattgcgaac cttcatcatt tcctcttcgg tcttgctgat aaggtcacct
     4261 acggtgttga tagcggctct ctttaagcag ttgtaggatc ttaccgaaag atccaaatca
     4321 tcgatgggtt tttccaagct ttcatccttg ttgatttctt cggcttcttt aacggtttcc
     4381 gtctgtttga cttcttccga aagatcaatg aacaaactga tatgatcgct cattatctta
     4441 gccgcataac tcaaagcctt tgtcggggac attgatttgt ccgtatatat ttccattgtg
     4501 agcttgtcat agtcggttat ctgtccgaca cgggtatcct ccactttata caccactctg
     4561 tttaccgggg tatatatcga gtcgatggga atggttccga taggcatgcc ttcttttttg
     4621 ttcttttcgg cggaatcata tcctctgcct ttggataccg ttatttccat gtagaaagat
     4681 ttacattcat ccatcttgaa aagataatga tcgggattga gtacttccac ttcggcattc
     4741 ttaagaatat ctcccgctgt aacatctccg gtaccttcgg ttcttatgct taaagtcttt
     4801 acttcatcgg tatccagtct gaacctgata cctttaacat taagaattat ctcggtaaca
     4861 tcttccacta ctccggggat tgtggaaaat tcatgcaata cgccgtctat ctttatgctc
     4921 gtagcagctg ctccgggcaa agaggataac aaaattcttc ttaaggagtt acccaatgta
     4981 gtgccgtagc ctctctcgag gggttccact acaaacttac cgtaaacatc ggttgtttca
     5041 acacattcga ttctcggttt ctctattgtt atatctatca atgttatacc ctccctttta
     5101 attaatggta tatttagcag ttactacttg gagtagaatt ccactataaa tctttcctct
     5161 acaggcagac ttacgtcttc acgtttacat gtatcgacaa tctttgcgct cacggcggac
     5221 tgatcaaact ctatgtgtgc cggtattatc ttggtctgtg aggtttcaat aagttcctta
     5281 aacttgggcg agtctttcat ggactccttg agttgtatga cttctcccac ctgtaccaga
     5341 tatgacggta tatccacagt tttaccgttt atggtaaagt gaccgtgctt aacaagctgt
     5401 cttgcttccg gtctggaatt agccagtcct atcctgtaaa ctacattgtc cagtctgctt
     5461 tccaatattt taagaaggtt ttcgccggtc ataccctttt gtctggaagc catctcaaaa
     5521 tactttctga actgtgattc caatacgccg taaaatcttc ttgctttctg tttttcacga
     5581 agctgcatac cgtattcgct ctgtttgctc cttctcttac cgtgctgtcc ggaagcataa
     5641 gctcttctta ccaaagcaca tttatcggta tagcatctgt caccttttaa aaacaatttt
     5701 gttccttcgc gtctgcaaag tttgcatact gcatctgtat atcttgccat attaactgta
     5761 cctccttaaa cccttcttct cttcggcggt ctgcagccgt tatgcggaat aggagttaca
     5821 tcctttatca ttacaacggt aaggcctgcg gtttccatag ccctgattgc cgattctctg
     5881 ccggcaccgg gtccttttat atatacttcc accgttctca agccgtgttc catagcagct
     5941 ttagcagctg tctctgcggc aacctgtgcg gcaaacggtg tgtttttctt ggagcctctg
     6001 aagcctaatc cgcctgcgct tgcccatgac aaaacattac cttgtaaatc cgacatggtt
     6061 acaattgtat tattgaagct ggaacggatg tgtgcctggc cgtgttcaac gtttttcttc
     6121 tcttttttct ttcttgcttt cttaacagtc gttttctgtg ccattgctca tgtctcctta
     6181 cttcttcctt acgctaacag atgctctgac gggaccctta cgtgtcctgg cattggtttt
     6241 tgttctctga ccccttacag gtaagcccat tctgtgtctt ctgcctctgt agcttcctat
     6301 atcaaccagt ctttttatgt tgaatgatac ttctctgcga agatcacctt caactacatg
     6361 ttccttttct atcaggcctc tcaattttac aatttcagac tctgtaagat cttttactct
     6421 ggtgtcagga tttactcctg cctgctccag aattttctgt gaactggtca gtccgatacc
     6481 gtatatgtat gtcagaccga tctctactct tttctctctg ggtaaatcca ccccggcaat
     6541 acgagccatt ttactttttt acctccatag tcctttcaaa atctttttga gtatctatgt
     6601 tatcactgcc aagaacagtt actcactgtc agccgcttat ggcagtgcta agcacattat
     6661 ccctgtcttt gcttgtgcct gggattatcg cagattacca ttactctgcc ttttcttttg
     6721 ataatcttgc acttttcgca tcttttcttt actgacggtt ttactttcat agtatatccc
     6781 tcctatttgc ctctccatac tatccgacct ttggtcagat catacggcga catctctatt
     6841 tttaccttat ctcccggtaa tatccttata tagtgcattc tcagtttgcc cgagatatgc
     6901 gccaatatca catgcttgtt ctgtaattcc acccgaaaca tcgtatttgg taaagcttca
     6961 attactacgc cttccgtctc aataacatcc tttgccaaaa tctttgcctc cttatcccgg
     7021 acactacatc cagccgtgtt tttcggagta tttcagtatt tccttccata catcctccgc
     7081 tacctgctct tgcggtttgt gcccgtctat atccacaaca attcctcttt ctctgtatac
     7141 cgcaagtatg ggttccgttt gaagtttata tgcctccagt cttgtgtttg ccacttcaac
     7201 ggtatcatct tttctggtat acagttctcc gttacataaa tcacagattc cgggtgtctt
     7261 tggaggatag gttacgatgt gatatgcttt tccgcattta cacattcttc tgccggaaat
     7321 ccttcttatg acttcatcat ccgttactga aagattaatg gccatattga tggatttatt
     7381 gcattccgta agcatcctgt ccaacgactg ctgctggttg attgttctcg gaaaaccgtc
     7441 aagcacaaat cctctttcac agtcttcctt ttcaatcctt tccttcaata tacgtattgt
     7501 cagctcgtcc ggaaccagta ttcctttgtc aatgtacttc ttcgcctctt ctcctaccgc
     7561 agtgttgttg gctatgttcc acctgaatat atcacctgtg gatatatgcg gtactttaag
     7621 tctttgtgct actataacgc tttgtgttcc cttgccgctg gcaggggctc ccagcataat
     7681 aacaatcatg gctacctcaa tcaaggaatc ctttgtaatg tctcatgagc atctgcgatt
     7741 ccatttgttt aaccgtttcc agtgcaacac ctactacgat aagtaaagca ctgcctccga
     7801 aacctatctg tattcctacc acctttgcaa gtattgacgg tattgttgcc aataaagcaa
     7861 ggaaaagtcc tccgaaccag gttattttat tcagtacata cgcaagataa tcggaagtgg
     7921 gttttcccgg tctaaagccc ggtatgaatc ctccgttctt cctcaagttg cttgctactt
     7981 ccaccggatt gaaagctatc atggtgtaga agaacgagaa gaagaatatc aatgcggcat
     8041 ataccgctaa aaatccccat gaatcggtct tttgcagtgc tatcaggaac ttgctttcgg
     8101 tattgggtgc tacgaagtta ataatgatgg aaggcaaagt agttatggat gaagcgaata
     8161 ttatcggcat aacgcccgac atattaagct gcatcggtat atgagtactt tgtcctccgt
     8221 acatctttct tcccactact ttctttgcgt agtttaccgg tatctttctt gtagctccgg
     8281 aaacataaac cgttcccact accataccta acattaccac taaaagaccg gttaccaaaa
     8341 gtatagtctc tatggctgta gctccgagat ttccctgttg ccagtttgca taaagtactg
     8401 cggcagcggt aggtattctg gatacgatac ccacgaagat tatcatggat attccgtttc
     8461 ctattccctt ttccgtaatc aattcgccca accacataac tattgcggta cctgcacaga
     8521 aagtaaatac cactgtaaag aaactcaata ccgttccgcc gtcatacagc acatcataat
     8581 ttttcaatgt tataaacagc atacttgcct ggaaaagtcc cagtcctatc gtaagatacc
     8641 tggtgtactg ggtaatcttt ttcctgcctt cttcaccttc tttagccagt ctttccaaag
     8701 cgggaatggc tactgtgaga aggttgagta taatggaagc attgatgtac ggctgtatgt
     8761 tcattgcaaa cagcgacata ttggagaaag ctcctccgga gaacatatcc atgaatccga
     8821 aaagcttgtt tccttccacc agtacttcaa atgccgcacc tatgagcccg ggaacgggca
     8881 ggtggcaccc tactctgtaa atgagcagca ttgctatggt gaacaacatt ttctttctca
     8941 gctcgggaat acgccaagca tttctgatag tctgaaacat tatattacct ctgcctttcc
     9001 tcctgcagcc gtaatttttt ctgcagcgga cttcgtgaat cttgaggctt taaccgtaag
     9061 attcttggtt aattcacctc ttcccaatat aactataccg tcgcagacgc ctttaactat
     9121 tccggcttcg cgaagcaatg cttcatcgac aaccgtctta ttcttaaata tgttgagcgt
     9181 ctctatatta acttcagcgt aattcttctg aaatctgtaa ttattgaaac cgaatttagg
     9241 tattcttctg tataaaggca tctgtccgcc ttcgaagcct actcttactc cgccgcccga
     9301 gcgggaattc tgtccgttgg ctcctctgcc gcaagtcttg ccgcttcccg atccgtgtcc
     9361 gcgacctttc cttgttgctt tcttctttga accttcggcc ggtgctaatt gatttaactt
     9421 cattttcagc tcctccctat tcttccacac tgacaagatg ccgtactttc tgtatcatgc
     9481 ctcttatctt ttcattgtct ttctgctcta cggtttgccc tattttacgc agtcccaaag
     9541 cggcaacagt agctttatgc ttctgtatgg aattgtttgc gcttttgacc aacgttattt
     9601 tcagcatttt agcctgttcg gcttttttct tagccatata gccctcctaa cccaatatct
     9661 cttcaacgga tttgtttctc ttcttggaaa catcctcaac cgtcatcatt ctggataatc
     9721 cgtctatggt cgcctttgcc agattaaccg cattatgcga tccgagacat ttcgtcctta
     9781 cgtcctttat gcctgcaagg tccagtaccg ctcttacggt accgccggct ataactcccg
     9841 ttccgggtgc cgccggttta agcagaactc tggctgcgcc gtactgaccg attatttcgt
     9901 gaggaaccgt tgttccgtcc aaaggaacgt ttataaggtt tttcttagca tcatctgtcc
     9961 ctttccttat ggattcggga atttcagccg cttttcccat tcctgcgcct acacggccgt
    10021 tttcgtcacc tacgacaacc aatgcgctga aacggaagtt tctgccgcct ttaacaactt
    10081 ttgctacacg gccgattcta actactttct ctttaaattc cgatggtgtt ctatcaaaat
    10141 cgtttctcat taaaacccgt tcctcctatt aaaattccag accggctgct cttgccgcat
    10201 cggcaagcgc tttgatcttt ccgtgatata catatccgcc tctgtcgaaa acaacatttt
    10261 ttatgccttt ttccagagct ttctttgcaa ccaatgtacc tacttctttg gcagcctcca
    10321 tgttgccgcc gtatttaagc tttcccttca aagactggtc cagcgtcgat gcggatacaa
    10381 tggtattacc tttagtatca tctataacct gagcataaat gttgtttaat gacttataca
    10441 cattaagtct gggtacttca gccgtaccgg aaaaatgatt tctcagcctg tcatgttttt
    10501 tctttcttgc tgcattctta tctgttttct ttatcatttc aattacgctc ctgtttttcc
    10561 ttctttacgt ctgattatct catcagagta cttaacgccc ttgcctaagt acggttcggg
    10621 ttttctgtat gccctgattt gggcggcaat ctgtccgaca gcttgtttgt cataccctct
    10681 tactattatg gataccggtg tgggaacttc aaatgttatt ccttcgggag gtataacttc
    10741 caacggttgg gaataaccta agttcagtac cagtttctgt ccgtcaagag cggccttgta
    10801 gccgacaccg tttatctcta atttcttttg atatccgtta accactccgt gcaccatgtt
    10861 attcaaaagg cttctggtta acccgtgcaa agctttttgt ttcttttcct cactttttct
    10921 ttcaatattt ataacatcgc catccttggt tatagtcatg ctcttattga aagtctgtgt
    10981 aagggttcct ttgggtcctt taaccgtaac ggtattgtct ttggctatat ccactattac
    11041 gcctgccgga acggttatcg gtaattttcc tatacgtgac atttatctaa ttcctccttg
    11101 ttcttaatat tgcgagcatc tgcccttttc agaaactacg aatacttatc cgcggttacc
    11161 aaacaaatgc caacacctct ccgcctacac ctgcttcacg ggccttcttg tccgtcataa
    11221 tacctttgga tgtggatatt atggcaattc ccaatccgtt aagcaccttg ggtaattcat
    11281 ccttgcttga ataaactctc aatccgggtt tgcttattct ctttataccg gttataacgt
    11341 ttttcttgtc ctgatacttg agagtaatac ggataactcc ctgtttctca tcgtcaatcc
    11401 actcgacatt ttcaatatat ccttcttcca aaagaatatc ggctatgtct cttttaagat
    11461 tggagccggg tatatccact ctctcatgct tggctgtacc ggcattcctt attcttgtaa
    11521 gcatatctgc tattacatct gaaatctgca tcttgttcct cctttaccag ctggctttcc
    11581 taatgccggg aatttcgcct ttatatgcca attccctgaa gcacagtctg catacgccgt
    11641 attttcttaa atatgcacga ggtcttccac agatctgaca tctgttgtac tctcttgtac
    11701 tgaacttttg aggtttccgc tgttttaata tcatcgcttt ctttgccaag atcttatcct
    11761 ccttatgctt tcttaaaagg catgccaagt tcggtaagca gccatcttgc ttcttcatcg
    11821 gtttttgccg ttgttacaaa aactaaattc attcctctta tcttatctat gttgtcataa
    11881 attatttccg ggaatatgag ctgttcttta agtcccatag catagtttcc tctgccgtcg
    11941 aaacccgtaa ggttaagacc cctgaagtct ctgactctgg gcaacgcaat gttgataagc
    12001 ttgtttgcga aatcatacat tcttgcacct ctcaaggtta ccataagacc tattttcatt
    12061 ccctgtctta ccttgaagtt tgcaaccgat ttctttgctg ttgttacaac gggtttctga
    12121 ccggtaatgg cggtaagatc ggccgctgcg gcatccattg ctttggggtt atctttggct
    12181 tctcccatac cgatactgac aactatctta acaagcttgg gcacctgatt gacattcgta
    12241 tatttgaatc tctccataag tgcgggaata gctttttctt tatataactg ctgtaatatc
    12301 atttttatcc tcctacttcg cgcctacttt gtcgataacc gcgccgcact tcttacaagt
    12361 cctctgctta ttaccgtctt tgtcaaatgt cattccggtt ttggatgcct gtttgcattt
    12421 ggggcaaata agcattacat tggatgcatg tatggggcgc tcgaaatcca ctataccgcc
    12481 ggcttctccg gcttttctgg gttttacatg cttcttgccc atatttgcat tttccacaat
    12541 gacagtacat tttgtcttgt ttacgtcctt gattttaccg gatttgccgg catcattacc
    12601 gctaagtata agtacgttat cacctttttt tacatgcatt ttcaaagtct cctttctata
    12661 gcacctcggg tgcgagagag actatcttca tatattcctt atctctcagt tctcttgcaa
    12721 caggtccgaa aatacgggtt ccgacaggtt ccttatccgc tttgatgata actgcggcat
    12781 tctcgtcgaa ttttatgtaa gatccgtctt ttctctttat gccttttttg ctccttacta
    12841 taacgcatct gacgatatca ccctttttaa caagcccgcc gggagatgcc gttttaacac
    12901 tgcatactat tacatcaccg atattggcat aatatcttcc cgttccgccc aatactttaa
    12961 agcagaacag ttcttttgct ccggtattat ccgctgcttt taatctggtc tgtgcctgta
    13021 tcatagtatt acctcgcctt ttccactatg gaaacaactc tccatctctt atctttagat
    13081 aaaggtcttg tttccataac tcttactctg tcaccgacag cacactcatt attctcgtca
    13141 tgagctttga gtttatacgt tctctttatg atttttttat aaagaggatg ttttacgctg
    13201 tctaccactg cgacaacaac ggttttatcc attttatcac taacaacatt tcccactctg
    13261 gtttttctca agtttctcgt tgtcatctca tatctcctta atctcgcgtt ctctgattat
    13321 tgtctttact ctggcaatat ccttcttaac gctcttcatt ttcatgggat tttccaactg
    13381 attggtagca tattggaatc tgagcttaaa aagttccgat ttaagttggt tcagttcgac
    13441 tgccagttct tcgttagtct tcttccttat atcattagcc ttcatttgcc tcaccacttt
    13501 ctcttgccat aatcttgcac tttatgggca tcttgccgct tgcaagcctt aatgcctctt
    13561 tggcatccgc ctcggcaaca ccgcctattt cgaacattac tctgcccggc ttaacaaccg
    13621 ctacccagta ttccggtgct ccttttccgc tacccatacg ggtctcggcg ggtttcttag
    13681 ttaccggctt atgcgggaat atctttaccc atacgctacc gcctcttttg atataacggg
    13741 tcatcgctat acgggcagcc tctatctgat tactcttaat ccaaccgggc tcggcagcta
    13801 caagaccgta atcaccctga tcaatggtgt ttcctcttaa agctttacct ttcattctgc
    13861 ctctgtggac tcttctgtat ttaacacgtt taggtataag cattattgta tacctccttc
    13921 atcatctttc tttgcgggaa gcacttcacc cttatatatc cagaccttaa ccccgatctt
    13981 tccgtatgtg gtatccgctt cggcaaatcc gtaatctata tcggctctta atgtctgaag
    14041 aggtattgta ccctcgtgat aatgctcact tcttgcaatt tccgcaccgc ccagtcttcc
    14101 cgaagccatg gtcttaattc cgagagcacc ctgtttcatg gttcttccca ttgcctgttt
    14161 catggctttt ctgaatgaaa ctcttttttc cagctgtgct gcaattcctt cggctacaag
    14221 ttgtgcatcc atatcgatgt ggctgacttc ggtaatattt accagcacgg tcttggtcgt
    14281 aagcttttca atatccgctc tgatctgttc cacaccggca ccgcctttac ctataatgag
    14341 gccgggtttg gctgcaagga tgttaatctt tactttattt gcggctcttt ctatctctat
    14401 tttagaaatg cccgctatat acagtttctt tttaataaac tttcttatgg tattgtcttc
    14461 aacaagatac tttgaatagt cctttttatt tgcataccat ttggtatccc attcttttat
    14521 aattcctact ctaagtccgt gaggattaac tttctgtccc atgctcaagc ctcctatact
    14581 ctttctttca gaacaaccgt caaatggcat gttcttttat tgattctggt agcgcttcct
    14641 cttgaaagag ctttaattct cttaagagtg gtaccctggt cagcatatat ttccgcaaca
    14701 tacaacttac tttcatcaag gttattatta ttaacggcat ttgcatgtgc cgatacaagc
    14761 agcttttcca aagtttcggt tatgatattg ggagtattct taacaatagc aagagcaact
    14821 tcataatcct ttcctctgat atggtctata acaagtttgg cttttgttgc cggaacggaa
    14881 acattattaa tgcttgctct tccctgatct tttccgacac ccagctcttt tctttgtttc
    14941 ttggtcaaac gaggagcttt tttgcacttt ctggtaaaag tcttttcctt attttcagtt
    15001 gttttttcca ttttttcctc cattatctaa ccctcgtagc cttatcgcct tttccgtgtc
    15061 ctctgtaagt ccttgtcaag acaaattctc cgagtttatg tcctaccatg tcctccgtga
    15121 tatatacggg aagatgcttt ctgccgtcgt gaacggctat tgtatgacct accatctgag
    15181 gatatatagt tgaggatctt gaccatgtct ttaagactct tttatcgtta gtctcattca
    15241 tcttctcaat cttttctaaa agcctcggaa atacatatgg cgctttctta tttgatctgc
    15301 tcatgtcaat tctcctttac ttgctgtgtc ttctcttgac aatcatactg tcagaagctt
    15361 ttttcttttt tcttgtcttg gtacccagtg tacgtttacc ccaaggtgta acggggctgg
    15421 gtcttccgat aggagctttg ccttctccgc caccgtgcgg atggtctacc gggttcattg
    15481 cggaacctct taccgtaggt ctgactccca tgtgtctttt ctttccggct tttcctatac
    15541 ggatattggc atgatcgaca tttcctacct gaccgatagt agccttgcag tttacgctta
    15601 ccattctcac ttcgcccgaa ggaagtctta tctgtgcaaa atcgccttct ttggacatca
    15661 attgtgcggt attgcctgcc gaccttacca tctgagctcc cttgccgggt tttaactcta
    15721 taccgtaaat catggtacct acggggatat ttgccaaagg caatgcgttg cccactttta
    15781 tatccgctgt cggtccggac attacggtgt catttaccgc taacccgacc ggagcgataa
    15841 tatatctctt ttctccgtcg gcatagaaaa gtaatgctat gttggaagtt ctgttgggat
    15901 catattcaat agctgcaact cttgcgggta ctccgtcttt atctctctta aaatctataa
    15961 ttctgtattt tcttgctgca ccgccgccct gatgtctgac tgttattctg ccgtgattat
    16021 ttctgccgcc ttctctttta ataggcgaaa gaagagactt ttttgaagct ttgccggtta
    16081 tatccttgaa tgaggatact gtcataaatc ttctggccgg tgaagtcggg ttatatttct
    16141 tgattggcat aatttactcc tttactacta atgcggtgtt acaccgaatt cttcaatgct
    16201 ggtcttatac ttctttgcaa ccgtcttaat cttgccgcct ttatcaagat aagtttccgg
    16261 ctttggatcg gtatcgattg ttacgacagc tttcttgaaa gaagcggttc tgcccagatg
    16321 cacacctact cttttttctt taccttggta atgcattaca ttcactttaa gaacctttac
    16381 cgagaacagt ttctccaccg cctgctttat ctcaatcttg gtagcttttt tgtccaccat
    16441 gaaagtatat cttcccagca tggcctgttc attggttctt tccgttatat acggtcttat
    16501 aataatatct tcataatatt tcattatgca tagacctcct gaatcttttc caccgcttct
    16561 tttgttgcaa caaatttgtc atatttaata atgtcataaa cattaatggt atttgtcaga
    16621 gcggttgcta cattaggcag atttcttgca gatttgacta ctatatcgtc tttttctcca
    16681 agaactacca gacaggatgt atcgactttg atattgttca aaatttctgc aaatttcttt
    16741 gtctttatct catccatttt gagactgtcc agaattataa aggagcctga gccgaacttc
    16801 tgcgacaaag ccgacttcag agcaagtctc ttctgttttt tgttcaacga gaacctgtaa
    16861 gatctcggct tgggtgcaaa cgcaactccg ccgcctatct gatgaggtcc taccgaagac
    16921 ccctgacgtg cgcgaccggt tcccttctgt ctgaaaggtt ttgtctttcc acctcttact
    16981 tcggctctgg tctttgtgct ttgtgtgccc tgtctggcgt ttgcaaggta ctgaaccact
    17041 gccgagtgta tcgcactttc acttacttcg actccgaata catcttcgtt caatgacata
    17101 gtgtcgacaa cttccccttt tatattatat acatctgctt ttaacattta ccgtgtcctc
    17161 ctatgcgttc ttggcatcct gaatcattat cagtccgccc ttgggtcccg gtattgcgcc
    17221 tttaactaaa accgcattac ggtccgcata aaccttcatt acctcaagat tctgaaccgt
    17281 aactctttcc gctcccatat gtcccggcat ctgtcttccc ttaaatactt tgctcggagt
    17341 acttgacatt gctaaagctc ccactccgcg atggtatttt gaaccgtgcg aagcacgacc
    17401 gatagctgtt ccgtacctct ttgtcgttcc ggcatatcct tttcctttgg atgtgcctat
    17461 tacatcgaca agcattcctt ctttaagagt ttccactgtt attacatcac cgactttata
    17521 tccttcggtt gaatccactc tgaattctct tacaaatttc ttgggtgtaa gacttgattt
    17581 ctcatacagt cccttttccg gcttatttac tctgctttct tttacatcac cgtaagccac
    17641 cttaacggca ttgtatccgt cgtgctcttt ggtctttatc tgcaccacgg tcaggggacc
    17701 ggcttctatt accgtaacag gtaccgcctg tccgtcttcc agaaacaact gtgtcattcc
    17761 cgcttttttt cctaacataa agtttttcac ttcgtatatc tatcctccta agttattggt
    17821 tcaactttct ttcagttgaa cttgactttt acaactttat ctctatatcc actcccgccg
    17881 gcaaatccag aagcattaat gcatccactg tcttgggcat agggttcaat atgtcaataa
    17941 ggcgcttgtg cgttctcatt tcgaactgtt ctcttgcatc tttgtacttg tgcggtgctc
    18001 ttaatatggt cactatttcc ttctcggtag gaaggggtac cggaccggat acctttgcac
    18061 ccgttctttt cgcagtctcc acaatcttgt tggcagactg atcgattaat tcgtggtcat
    18121 aagctttaag tcttattctg attctctgtt tgctagccat tctgatagct cctccttaaa
    18181 ttcttttacc attcctgtta gcacatggtc ccgggtgtat cgcgccttcc cattatatat
    18241 gcccgtatga cacgggccgt gacctttgtc taatcgcttc cgcccaacct tatagattgg
    18301 tacttctccc cttaagttac ccgcaaaatg cggcaatctc aaggatcatg gtacttgcga
    18361 aaatatcgca gcctacaaat aatactacat ccctcagctt tttgcaacac ctttttttat
    18421 aaaagcctgt tttacatcga tttaacattt tctttacgtg tagtttataa aacattgatt
    18481 agataaatat ttaatgatat tatatatata gtcgaactgt actaatttaa taatgcacgg
    18541 aatttcaaaa ggagggtaac tatgaaagat ttttatggac gaaaagcaac gcttgttaag
    18601 cttgccattt gcgttgcgct gttttgatta gcgccctggt cggatcgttt gttcaaacgg
    18661 ccggctggac agcgaagatt gaagatttgc gcaacaaaac caacaccggc accatcacta
    18721 tcaaggctac ggatagcgag gaggccaaag actatacggt caacggtaaa gttgtttccg
    18781 gtattctgat tacacctaaa aatgccacaa aagatcatcc ggcacccggt gtggtcttta
    18841 cacacggtct gttcaacaac cgtgaaatgc agttgcaaaa cggcatcgag gctgcccgca
    18901 gaggttatgt cgtattgctg attgaccgtg aatctcacgg acacaacgac acaaccactt
    18961 atgacaatta cggtgattcg atgttgtccg cagccaagta cctttacaac ctgaccgatc
    19021 cgcaaggtaa cagaattgtc gacggcagca ggattttggt ttccggtcac tccatgggcg
    19081 gcagcgccac aaacggtgct ttgatgcttg acggtgtatc caataagacc gctatcaccg
    19141 taggcgacac caagtacaca tgggaaggcc aggttgatga agcattgagc aaaggatacc
    19201 acatgggtat tatctccgca ggtatcgtcc aggccaacaa tgctcagaac gccagttacg
    19261 gtaccaacct taaaggtgtt tcggttctca aggcatccag cgacgagttc ttctttgcca
    19321 gcacaaccaa gaacggaatt tatcgcccgc tcaacctcaa ccaaaacact gaattgttgt
    19381 tcacacaagg tataaacgaa aaggttacaa caaacagaag tttagacggt aaactgtatg
    19441 tgaagaaggg ggatgattat gtagcaataa cctcttccga tacttatcac aagaaaacac
    19501 aatactatgt atttacaaag aacggtaatt ctccgttcta cctcatgtcc tcgcaggcca
    19561 tgaactttgt gggcaaacct ctggaatcgg caacaggcga acaatatgaa gttattaacg
    19621 gcggaatcta tgacttcaat aaagaaggag ccctgatagg tcagcctaaa gacaacaaat
    19681 acctgtcggc acagaccggc ggagcgcaac aggcaagtga tactgttcag attcgtgcag
    19741 tttacgaggc caaagaaaat cacccgatga atcacttctc cagaatgagt gcagcgggaa
    19801 taatagactt ctgctacaac gtattcggag ttgccgaggg cacaacattt aaggcaccta
    19861 ataaccaaac atggtggatt aaagaagtat tcgccatttt cggaattgcc ggtctgttcg
    19921 gactgcttgt catactggta gatttactgc ttaagacaaa agcattcgct tcactggcag
    19981 ctgccgaagg cgatattccc gaggctccgg ttcttcttac gaagccccgc aaacatgtat
    20041 tctactggct gagcggtatt ctgactgcat ggtacggata ttatgcctgg gagaatatag
    20101 tgaggggtaa aaactggtat gcaaacagct tctggagaac actgattacc gacaccacaa
    20161 gcgaaatcac cggtcagacc tacaccagct gggcaaatgc cggtaccatt gcttactggg
    20221 gtatatgctg tgcaacattt gcactgataa taacggcggt catctggctc ataaacagag
    20281 tcatcaacat gattgtttac aaagatgatt atcttgcaca tgaagagaga ccgttcctgg
    20341 gcttcagaat tcgcagtgta ggtaatatct tcaagacgct tgcacttgca gcaatacttt
    20401 gcttcgtgtt cttcggaacc atacaactgc tttggaatgt gttggttgtg gacttcagag
    20461 catggacatt tgcattcaga atatttaaga ccgacagact gctgtcctac ttaagatatg
    20521 tgccgttctt ctttgtattc tacatgacca atgcggcatt gaatataaac tacagggtga
    20581 aggatctgcc cgagtgggca accgttctca taaatgtagt attcaatgta ggctggttga
    20641 ttataagtgt gtggtacttc aacagctact tcatcaatgt aggagcattc acaaacagca
    20701 cgttaagcat gcagaatata tggacatatc ccatcattcc atgcgttgca accgcaacta
    20761 ttgtttccag acgtctgtat gtacgtacag gaaatgcatg gcttgccggt cttgtaaatg
    20821 ccactataat ggcactggtc tgctgtgcag gcacatccat aggcgcataa gagaaacggt
    20881 aattcacaaa agataaatca aacggaacga gaaatcgttc cgtttttcta ccttatatat
    20941 tatataaaat ttatataaat accttatata taactatatt aaccaacaac aaaattatat
    21001 aaccctgata aactcctact tacgcaaaac aacagtcaac aaattgccac taccttttct
    21061 aaaaacttga acactcatac agcttattca aatccttatt tttatcacgg acatatctgt
    21121 ttacgatttt agaaaaccac tccgtctcta tttcattcct tttttagcaa ggtctcaaat
    21181 cgctctttcc aaatcataga cttttacaga tttttcgaaa acggttttac attccgtaac
    21241 acccggttca taaattggtt ttgttacatt gtgaatcctt acatttccgc ttatgcgatg
    21301 agaattgtag tctctccaca ctgtaatttc gagctcttga gggattattg tccgtatatt
    21361 gatggagata cggacctgag actataaaag cacatatggc tgattgcaac tataatattt
    21421 gatggttatc aatatatctt ttcagctgct gatagaaatt ctcgcgtgtt cctgccaata
    21481 tcacaacaat aaacttctcg ttctgctcaa atatcctata cgcaatttca taatttacac
    21541 tactataata tacattatag caatatattc ctgccaaatt tcctttctta atctctccgc
    21601 tataaggatt cttgctgatt ttgtttatag cttcacggta ttcgttcaat agcttttttt
    21661 ctttaacttt cttaaagtat ttctctgctg cagaagtaaa acggatttca tacattactc
    21721 gtccacctgc ccgaatactt cttccattgt agcgcctttt ttcttgccgg ctgctatgtc
    21781 gtcagcttct tttaaaagat tctccaatgc ggatttaatt ttctttctct tttccttgaa
    21841 tttaattatc aattcctgtc ctccgtaacc ttgctcaact aactccgaaa gaataaaatc
    21901 cgaatattca tccgccatgt tcacactaat aggacgaatc aatattgaat cttcctgtaa
    21961 aacacattcg acctcattgc ctaaatccaa ggcttcaaaa aacttttggg gaatggttat
    22021 ctgtctcttc tttgaaacgc ttatgatttt cctttttcgt tgattgtttt ccaaataact
    22081 gatcataatt acaacatatc ctccttatgt aaacttgcaa acagtaaaga aatatgggtt
    22141 tctttgttta tattatatcc aagaaatact gattttacaa gataattcta ccactcccgc
    22201 atattccgga actcaaatga tttggcatct aattccgata tggataactt gcaacaaaag
    22261 ctattagagg gtaattatcc cggtgttagt ggatattgaa gtagcagctt aaatgaaaat
    22321 acggactata ccgacctaaa taaaaaatat gcgtcaattt tatctaacat ttccaaatgg
    22381 ctacgcactg cgtggcgaat tgagctggac acattatcgc agacaaataa acagcttttc
    22441 ctatgaaagg ttgctatcaa gtcaaaatcg cctctctaaa ataaaggtgc ttgtatataa
    22501 gactatatga aataagccgt accacattga atcatttagt gtaaattgtt ccgcgaatag
    22561 aatcccattt tagtgctgct gataccaaaa catttgtgtc aattacagca taatattttc
    22621 taacagccat ttcacttcct tgcctctgca atttctgcat taatctcatc caaagtcata
    22681 tcggagattc tgttctcttt tgcaatttta cttgctcgtt tcatagctaa aactccctta
    22741 ttaacattta tgtcttcgag tttcatactg aatggaatac cgccttcttt aaccagtctg
    22801 gatacgcaca ttctaaagta agtcggaaga tcaatgccca gatgcatgca tatctgtgct
    22861 gcctctgctc tttcagcttc atctatccta aattggacca gtgtatttgc cataacgcat
    22921 ccctcctttg catattgatt atatcatcat atgcatgcat atgcaatcaa atgtttatat
    22981 tcaacattct aattcattat atttccggtt aattctgttt acttcttaaa caaccattcc
    23041 ggcagtttct tatcaggaga ttggaaagga tggtagcgta cgggaatatc caaataccat
    23101 gattttttaa gtatactctt tgtgtgtgag aaggtctcaa aggtctcttt accgtgatat
    23161 ctgcccattc cgctgtttcc cacccctccg aacggcagat gcggactgct taaatgcata
    23221 accgtgtcat tgacacaacc gccgccgaaa gaaagatttt tcagaattgt tttctctacc
    23281 gatttatctt ttgtaaaaag gtacagagcc aaaggcttgg gacggctctt aacaaagttt
    23341 acagcctcgt ttatatcgtc aaaacctatt acaggaagaa caggaccgaa aatttcctcc
    23401 tgcataagag aactttcggt atccggttcg tttactaaag tgaactctat ttgcagagta
    23461 tcgggatatg tatttccccc aatcaaaatg tcttgggttt ccataagttg cctgatacga
    23521 tcaaaatgct ttttgtttat aatcttgggg aaattttctc ttttgtattt tgtgtcggga
    23581 atcattgtat tgtaatgatt ctttatttca ttgataagtt ccgcctttat gctgttttgc
    23641 actaaaagat agtcgggagc tacacaggtt tgccctgcgt tcagaagttt tccgaagagt
    23701 atccgcttgg ccgttttttt aatatccgcg ctttgttccg ctattacggg actctttccg
    23761 cccagttcca gagtaacggg tgtaagatta tttgcagcct gttgcattac ggtttttcct
    23821 acagcgggtg atccggtaaa gaagatatag tcaaaatgct gctccaataa agcgttgttc
    23881 tcctctctgc ctccttccac aacggataca tattccggag gaaatgcttc gcttataacg
    23941 gagcaaactg catttgatac agcaggagca tatgccgaag gctttacgac agccgtatta
    24001 cctgcggcta tggcgcctat aagcggaacc atgcaaagtt gaaaaggata attccaaggg
    24061 gccattatga gaacagtacc gtaaggctcc tttattatct tcgctttagc cggagcctgt
    24121 gaaagagcaa ctttctttct ttcaggtctt gcccaatgtt ttatattttt taaagtatgg
    24181 tccaactcgc aaaatacaat tgcaagttcg gtcatatatc cttcataacg gctcttgttc
    24241 aaatcgcttt tcagagcgga aagaatcata tcttcattat ttcttatgga tgtttccagc
    24301 agtttaagag atttaatcct tgcacttatc ggcaaagtgt gtccttcatt gaaataaatg
    24361 cgctcttttt ctacaatctt atttataatt gtgctgttgc tcatgtcgga aactccttta
    24421 tcggtttcta taccacctgt tttgcgtact ccaatactct ttcctttacc atctcgcagt
    24481 tggttttatt acagtattcc aatatgaaag cggcatcggg ggtatgcttt ttacgaagcg
    24541 acagaaactt cacccagtct atatcgcctt ctcccacatt cctgcccttt gcatcattta
    24601 cttttctgtc tttaccgtga aaatacgcaa tatagtcttt aagatatttg aatatatctt
    24661 cttcatcgga attggcaatg agatttgcgg gatctaacaa tattttgaag ttatctcttt
    24721 cacactgcca gattaagtct ctggttcttt ttgcggaagg tgcaaaatca aatatacagg
    24781 gctcatatgc aatggatact ccgtaatgct ttgcgatatc caacagtctt atcatgttgg
    24841 atttgaaata ggcaaaatcc gcctcaaagg taaagaagtc tatgcctctt tttcccggaa
    24901 caaatcccga ttcggttgcc acatactcaa taccgttgtt tgcggctacc tgaatgtatc
    24961 tttcatatgc tttaaaattg ttttcctgct tctccttatc cggctcgaac agtgaggaaa
    25021 atgcacctat ggcaactaca tctatcgaat tatccctgaa caatttgatt atgtctttag
    25081 cggcttcatc ggtcaacgca tccatcggtt tgtaaccgtt gtactggtat acatataaat
    25141 tactgaatgc aaaacttatc tctgtagagg taaaaccgaa tttctttaat aagtctaccg
    25201 tttcctccgg tgtatgattt gcaagactcc tcgtaaccaa tcctacttcc attgtgtaat
    25261 tcctcctcta atccttttta attatatctt ccggtacaat ccatcctttt ttatagaaga
    25321 tattgagaac cggtctgata actaaaaaaa taaccagcgt ttctatcact atcaccgcta
    25381 cggtcttaat gcttcttgct accaataatt cattaatcgg tattctgtac agcatataca
    25441 atgctatact cataagaaaa aagtcgctta tcagggcttt ggtcagtacg gcaaaaattg
    25501 tggtttttac ggttatttct ttattgtgaa gaaataagcc gtatataagg ccgttagcca
    25561 tggccacaat ggttattacc ggagaatacg gtccggtagg aaataaaacg cttcccagca
    25621 aatcggcaag cacggcggat aatgctccgt aggcggggcc gaataatatt gatgccatag
    25681 caatggaaac aaattccgtc cctatcctta taacctgaag attaacggct aagagttttg
    25741 ctaaaacaac cgtaagtgcc gttaataacg acagtatcgt aattcgtctt gtgttgctca
    25801 cttgacggct ccttttatac aatactcttc cagaagttta agatatatcc ggtaattatc
    25861 cagagaaact tccggaggtg tctgatggtc cacactgggt atataccctc ccgacctcat
    25921 ggccggaagc agacgctcga attcttcccg catagcctgc tcggaaacac tcatcaccat
    25981 cttgtcgtat gccccgatta tgagaaaatc gggatgattc tttcttactc tgttgatatc
    26041 cactcccgct tttctttcca gaggcaaagc tcctcttata cccgctcttt tcagccacgg
    26101 aatcattgct tccagctgcc cgtcggtatc cactataggt attattcccg cttcttcaat
    26161 atgcggaacg gtctgcctgt agtacggaag caggaactca tcaaacatct cttcggaaag
    26221 catgggtccc aaattatatg acatatcttc ggcaaatgtc ataaagtcgg gctgtatggc
    26281 tttgctcatc tcgtcaatta catagtttat gtagtccgct tcatcctgat tcatgcggtg
    26341 catcagttcg ggctcatcat agaaggcata taaatgctcc tctataccga aaagcgttct
    26401 cggaaaccag aagaagccgt ccaccgttat ccataccacc aaatcaccgt catcatgcat
    26461 ttttttatat tgtgccgcat cttccgccgc agcctgtact ttccttttat cataaagata
    26521 gggttttaat ctttcatacg actgtatgtc ggttactttc ttcatatcct cggataactg
    26581 attatatcct ctccccctgg ctcctatcca atactgtttc aatctgtcca gcccgaagta
    26641 ctcatgcttt atgtcttctt tattgggcat atcgggaacg ccttcggaaa tgaatctttg
    26701 aatggtttta tcccaccatc ccgcccattc aatcatgggc aacctgtcca cgggtcggaa
    26761 attcataaca tttaaaaatc tttctctctg gttcatatta atcacttggc aatggaaaca
    26821 aattccgccc ctttctttat aacctgaaga ttaacggcta aaggttttgc aaaaaaaacc
    26881 gtaagtgccg ttaataacga cagtatcgta attggtcctg tgttgctcac ttgacagctc
    26941 cttttataca atactcttcc agaagtttaa gatatatccg gtaattatcc agagaaactt
    27001 ccggaggtgt ctgatggtcc acactgggta tataccctcc cgacctcata acaggaagca
    27061 gacgctcgaa ttcctcccgc atagcctgtt cggaaacact catcaccatc ttgtcgtatc
    27121 ccccgattat gagaaaatcg ggataattct ttcttactct gttgatatcc actccggctt
    27181 ttctttccaa tggcaaagct cctctgattc ccgccctttt cagccacgga atcattgctt
    27241 ccagttgtcc gtcggtatcc actataggta tgattcccgc ttcttctaca cgcggaacgg
    27301 tctgcctgta gtacggaagc agaaactcgt caaacatctc ttcggaaagc atagggccca
    27361 aattatatga catatcttcg gcaaatgtca taaagtcggg ctgtattgtt tcacttacct
    27421 cgtcaattat atagtttata aaatccgctt ggtcctgatt catgcggtgc atcagctcgg
    27481 gtttatcata aaaggcaaat aaatgctcct ctatgccgaa aagtgttctg ggaaagtaaa
    27541 agaatccgtc caccgttatc cacatcacca aatcaccgtc atcatgcatt tttttgtatt
    27601 gcgccgcatc ttccacagca acctgtgctc tgtacttatc ataaagatac ggttttaatc
    27661 tttcatagga ttcatcgtcg gttactttct taatttcatc ggataactga taataccctc
    27721 tccccctggg ttttatccag tactgcttca acctgtccag cccgaagtat tcatgcttta
    27781 tgtcttcttt attgggcata tcgggaacac cttcggaaat gaaacgcaag attgttttat
    27841 cccaccaacc ggcccattca atcatgggca acctgtccac gggttggaaa ttcataacat
    27901 ttaaaaatct ctctctttga ttcatagaaa ctcctcaacg gttatattac cagtccgtaa
    27961 ttgcttagtc aatttgtttt gactgtaata acttttcaaa ttcctcatat ggcatgggtt
    28021 gataatagta gtaaccctga gcgatatcac aatcaagcga ccttagtata tcggcttgct
    28081 cttttgtttc cacaccttcg catataatta atgaatccat attatgtgca agatcggtta
    28141 tgccttttat catctttttg gttttactgt cttctaagtt gtatatcatg cttctgtcaa
    28201 gtttgagata gtccaacatg aaattgggca aatcggagaa ggaggaatac cccgttccga
    28261 aatcgtccaa tgaaatggta aagcccattt cttttagttt attgactata tctttcatca
    28321 tcttcgcatt ttccgcaata agactttccg ttacttcaat gttcagataa ttgggagaca
    28381 cttggtattt tgtcgctatg gcaaatatcc ggtcgcagaa atcgatattt gcaaagtgcg
    28441 aacgtgcaaa attaaccgac acgggaacaa cctgtctgcc ttctttaaga ctctgttgca
    28501 acagttggca agccttttca aacatgtaca tatcaaatgt cgtaataata ccgctctttt
    28561 caaaaacagg tatatattcg cccggccgga taagtccgcg tctggggtta taccatctgg
    28621 acaatatctc gccgccgtat attgagttgt cggcaagcag tatcttaggc tgtatatata
    28681 tgaggaattc gtctttttcc atgcttgcaa ggaattcctg ttgcataagt ttttcctcta
    28741 cggtcataat acgaacggta tcatcataaa acttgtatct aacatcatcc ttttccttta
    28801 cgaactgacg ggcctgcaaa gatttctgca taaggacatc catattcagt tcttcatatt
    28861 taggattata aattcctata ctcagcacaa cgggataatc ggagttttta agattgtact
    28921 tttcatttac cttctctact atattggata atctccctat catttcctct ctttcggtaa
    28981 attgaagcaa caatatgaaa tcgtcgttat ttgctcttgc aagaatctca ttgttgtaaa
    29041 tatgattatc cagcagtacg gctatatcgt tctgaacctt gtccgcctct gtatatccgt
    29101 aaacctccct tatgtaattc aggttataca caacaagatt aaccatgcag tatgtactta
    29161 tattcaacat actgatggaa acatcggtta ccgctttcat cccccttata ttgggatacc
    29221 ctgtaagact gtcggtattc agaaaaagat tcttattctt cttatacttg aaatatgcaa
    29281 taagtatcgt agcacctgtc agtattgcgg atataactac cgagagagca atcgtgacac
    29341 gcagttcggt tctgttgaac attttttcat actgcacggc ctcgcttgcg ataatggtct
    29401 ttatatcttc ctcggaaatc gtatctatgg cacggttaat caacgcggca acatcgggcg
    29461 gcattctttg ggtaaactgc atccccacat cataaccttt gaattttacc aactgtctgc
    29521 tttgcatgaa gccggcatac ctgtttactt tcgatacggt aatatccgcc tgaaagttaa
    29581 atccgtattg cagggcttgt tcaaaatctt ttgttttgag atattcgaat tgaaatccgg
    29641 tcagctgatg tacatggtct aaaatccgta cgacaacacc ttcatattgt ttctccgcat
    29701 agttgtagaa ttcaaaagga aaactatttc tgtccagtgc cacatagaca acggggtaag
    29761 atgccacaac ggcctgttct tcctttgtca gcacaaaggt ttgttcctcg gccgcatatc
    29821 cgtatattgc agacattacc agaatacata tgattgataa aactaaaaat ctcttcttca
    29881 tctactctcc tatgctcagt ttatgtgctt tatcacagtc ctttttgatg ccttccgctc
    29941 cccttatgaa gcttttaagg actttggata ccgattgcct gaattcctcg aactcttcct
    30001 ctacctgttt atattgctcg acctgacgct ctttttcctc cattgtgtct ttcagtatta
    30061 tatcggcctg ctttcttgca tcggccaata tattgtccgc ctttttcctt gcatgaatca
    30121 aagaaagagc aatatcatcc ttattcttct ccagcagttc cagcctgtta agcagcattt
    30181 tgttctgctc cagtataaga ttgacctgcc tgtctttttc cgctatgcga ctgttcagat
    30241 cttcctgcaa gagttctatg tacataatga catctttttt tgaatatccg aataaaactt
    30301 ttttaatacc tttgaatgca atcatatctg ttatccttta tcttgtttat atacattgta
    30361 ctacatactt tgctttacac actttatatt atcggttgaa ttttaccctt tttattttac
    30421 cccactgcag ttttttcttc ttataaccta caaatgcgac cattctggta taggctaata
    30481 ttactctgaa tccgatattt tcaaatatac atgcaccgat tgccttccac acatcctttg
    30541 cggtcaacct tatgctctgt gtatgtatcc tggagaaaaa ggcggataat gtcaataccg
    30601 ctccgtaaag taatgatatt atcgtgtatg ctatggcaac ccttgtagtg accatgccca
    30661 gaagaaacat ggctataacc gacaatatac cgaatatctc tatataagga gatagcaact
    30721 cgaagataag aaaatacatg taggataccg ttctgaccaa tccgaattta ctgctgaaaa
    30781 gtatctgttt gtgcttggtc agactctgat acagtcccag atgccatctt cttctctgtt
    30841 ttctcagatc tctcatgtta tcgggagcct ggctccagca taccgcatcg gtggcatact
    30901 ttatggtata cggtatttga ttcttaagac aaaacacatg cagccgcaca accagttcca
    30961 tatcctctcc catggtggtg tgatcataac ctccgaccgc aattaccata tcctttttaa
    31021 ataatccgaa tgccccggat attatcaggc ttccgttgaa catatcaaac agtatcctgg
    31081 atgccagaaa agaccgttca tactccaaaa cctgcattgc cgcaaccata ttgtccggca
    31141 tttcgtactt tttgactttt ccgttttcca gcaccacccc gttggctatc cttataactc
    31201 ctccgcatgc aataagattc tcatcggtaa gtaccggccg gactattctc tcaagagcgt
    31261 ccgactgcag catggaatcg gcgtccatac acacgaaata aggatatctc gatgcgttta
    31321 tacccatgtt taaagcatcc gccttgccgc cgtttttctt tcttatgagt acgatgggaa
    31381 ctttatatgc atagctttca tatatgaatt cctcgtcctg gcaccgtatg cttttcttta
    31441 tttttctccg caccttggtc atcttgaact cttcgataag cagtttggaa gtgttgtctt
    31501 ttgaaccgtc atccacaacg ataatttcat agagtctgta gtcaagattt aaaagagagt
    31561 gtacggtatc gcaaatggtg acttcttcgt tgtatccggg tactacgatg gatacgggga
    31621 aatagtaatc atgtgaaatg acattatgca gtgtgttcaa ctgcttgctc ttgtacaatt
    31681 ccgaagctcc gatggttacc gataccagca aaaatgtagc atatgcaaaa aggtaaagag
    31741 agtaaaaaat ccctattgat ataagtattg ctcttgctgt ctcaataaac atcccgtttc
    31801 tcctttctcg tttttctctt gagcctttcg gtttccatct tgtactccag tatttccctt
    31861 gcgtatctgt ccggtccgtt atatatatcc accaaatcca catattccac atccagctct
    31921 acaaggcttt ccgacgcatt gaatcgcaca taccagttgt ccgatgtcaa tgcttctttc
    31981 agtgcattca ccgtttcatc gtttttgtat gatgcaagag aggttgcggc tattgccctg
    32041 tattcccact cggcattttt ctgcctcaga agctccacaa gatattgccc ggcgggagca
    32101 taagtcatcc ttccgaagta ccttattatt gcaagcctta tttccttgtg ggaatgctcg
    32161 tcttttaaca actttaagaa cttttcgctg taatccagag tggacatcct tatgaaatta
    32221 attatggcaa ccctggtgct tacatcgaaa cggctgtatt ccttccacaa aagcgatgca
    32281 aggcgtgctc tgtctccatg gaagagcatt aatccgtcgg atataagttt gttgttgtgt
    32341 tttattttca tttcctgcat ccgaagcatc gcggtaagta tggcttcgcc gttatcggat
    32401 gtatacagtg catgaagagc attctgcctt acatataccg atttattggt tgtataggat
    32461 atcattgccg aaacaatgaa gtcataaacc gtttcatcac acagtttgaa ctcgccgaca
    32521 agaaaagcaa gataaccttt tttagaattt tccttgttat cgaactctat aataagatat
    32581 tcaaacagcg gtttacacag tttaagatat gtgaatgtgc tctcataatc tacagccgct
    32641 tgctctttta caatctcatg aaatgctttt aagaaacgta tcttagtgaa attcctgtac
    32701 agatactgct gcttttgttt gtcaatctcc tcattcttac ttacaagctc aatctgatct
    32761 tttattgcct gcctgatttt ttctctttta tctttgaata taaagtcccg gtagcgcaga
    32821 tagagaagaa agaccgtgtt gaaacaaatc atcatcacac agattgccgt gtatataatc
    32881 aatatgagat ctatccttac ctcattcgcc atccgctgtc cgctcctcgt ctgtccacat
    32941 tttttgtatc atgtaatatg atgctttaag tctttcatga tatgttacct tgttccatcc
    33001 ttttaaactg aacgggaaca tttctattat atgccctctg ctgtccgctg accctatacc
    33061 cacatacatc ttatctatac ctatgctttc caccccgtaa tgctcataat agtcgtcatg
    33121 aataaacata ttggacggat cggaaaaatt cagaagattc cagggaattc tcaactctat
    33181 atagttgccg gcaatatgga aatcggccag cgaattgtat tctttatgtg cggggttgct
    33241 gtttccgtgt gtcaatattc ccgtttcata aacttgcgcc ttaatgccgg gatggttgac
    33301 tttttccaat gcttccagct gaagtatgag atttatctta ttgaataccg ggctgtttat
    33361 gtgcggagga cgggaatgcg gattctctcc cgctatatac tggttataca tcgctctcag
    33421 cacatcatat cgttcctgaa ccagtaccct gctatcattt atcccgttta atactataag
    33481 aaagtccgca tagcaatcaa accgcacatc atgttgctta cagtagttgc tcccggattt
    33541 tcgggtaata tctatgggta tgtaaagaat atcgcgttca cggtcataat cctttttata
    33601 tacaaggtaa tatatgaact tttcgtcata ctgcattgat atgctcatat caccgtttac
    33661 cccaaccggt acaataccga accattcgct taaatccccg tcaacatgac acaatacttt
    33721 tttctctccc gggtcaaaag ccaatatacc gaatccctga tcggaagact gtgcatccga
    33781 ccagtacaca caccttctct catccaccga atgttgtgtg ttccaggtgg tcagcgacca
    33841 gtcatcctgc cagacataca caaggcttcc ggcacaatcc accgatttta tctgctcata
    33901 tgtattaact attatctgtc cctgttcata ttccgtattc ctgccctggt tatacccttt
    33961 gtttgtatcc tcatgagcca tccctctgga ggtaggcact ccgaactcgg ttataactat
    34021 gggaatgctg tgatggagtt taagcatttt cagatatgcc gtataggtgt tgacattacc
    34081 ttccgcatcc ttaaaccaga aatccttatt ttcaaaatag ttaaggtaac cggggtaata
    34141 agggtaagca tgataggatg cgaaatgacc ggatataaat gcatcggtcg tccttatatg
    34201 ctccacatcc acctcggcac actttttgaa aaattccgtt atataatcgg gatatacgaa
    34261 aggatcggta gacggccagt taaagaaaga taccaacctc tgctctttat accttgccga
    34321 ttcgtaagtg attatggtat cgcctatcat tgcaagcacc gattcaaatg ccgtcgcatc
    34381 ttccgtagta tacatatact tgccgctgta tccttcatgt actttatgca acctgtcggt
    34441 atatgcgatg gtattgctgt tccagttgga ccccaatata tatcccaaca cccagggcga
    34501 gatatctttc atatagaatc cgtaaccgct ttgctgatta agtaccagtt ggcgttttcc
    34561 gtgtattata tccacaagaa tatatgtctg ctttattatc tctttataaa aatcttcatc
    34621 tttcgcatct atatgactgt tctggacata atcggaaacg gtaaggccgt gaagaagata
    34681 aagagggtct tcattatctt tattaaaatc atacagcgca ttatagaagt ctacattcat
    34741 tatctgatac acccttacgg tattggcgcc catttctttt atctgtttta accacctgag
    34801 gtaatcatca tattctatgg catagtcggt tccgaatttg ccgggtatgg cacttcggat
    34861 atttacacct ttaatctgaa atttctcata ttgtccgttt ttcagaatgt atatgttttc
    34921 ctcatcggta atgaatgacg tcgtaacagg tgtcttaggg tgtaaatcta tgaaaaagtt
    34981 cgtggagaac agcacatagt ttatacctaa tgcaagaatc aatataatac ccgttgctat
    35041 taaaaacttc ttcatttaac ctctttacat gcctaattat tgaaggactt gtttctggaa
    35101 tcgtggctgt actgttttat cgaatcaata ttctcatcca tccatttccc tcttgccgtt
    35161 atatagttct taagctgatt caatgccgca tcgtaacttt tgagatttct ttctgcaggc
    35221 tccagcaatc ctttatctat taagaaagta tatccccact tatcaaaatt tctgtcaata
    35281 gcaggtccga gatattttac ggtcttgtcg atatagttta ttaaatactc gtcgctcagc
    35341 accgtttttc ttaaaaactt atatctttta attaccaaat ctgtaaaata gtcgtctttc
    35401 atgagcatga tgtaccatac tttattctgg aaggaaaatc cggtagcact catgggtgtt
    35461 tccatatagt tatcataagc attgttaaaa tcccataccg gacctattcc tatcttccct
    35521 tttgtatctc tgtaaagata tgtggagtaa attcccgcat cataattttg cgtaaactcg
    35581 tttataatca aataatccac gaaagacatt acatcaatat gtttacggta gcctgattct
    35641 ttatcggcat aatcatagga gtataaagct ttttcaaatg ccgatatatc gttttcaata
    35701 tatgtcttta tctgctcgtt aatgtttttt cttgcgggat agacaatatt acatatggaa
    35761 ttcatgttgt atgtatatcc ggtaaacggg tctatctcct cttcgggaac ggtacctctg
    35821 tccagccgga caatataact tgtaaacggt ctgtcttctc tgtatctgga aatatccacc
    35881 ctgcctttgc cgcgtgagat tgcttccatc atgacatata cccccatata ttcatcattg
    35941 acaaatacct cgcaaaaacg gacatcggga gcattactca ttatctctcc ggctatattc
    36001 atgcttatat aattccttat gagtgtcttg tccagtatag gtccgttgag aatccactta
    36061 tcatgggctt ccatacccat tacttttttg gcgttctctg tacggtcctc ttttataagt
    36121 cttataagat aggatttttt atcaaaccat aaggaagtgt tgcctcttgc tcttatggta
    36181 atgagactgt gcaaatccgc ctgatcgtcc agatgattgt tgctatcgtc cttgtcaaat
    36241 atcgaaaggt ccgcatcgat aaccatgctg ttatcggcaa gaaccggtat gtcctgtccg
    36301 tccgtattta tatgcactaa aggaagatga gtacagaagc gaccgtccgt atgattacac
    36361 ttcacatgct ttacatcgtc aatatgctga tgatgtcttc tgttgccata atttaagtaa
    36421 gacagcgaaa cggctacggc aataaggcat ataagggcta ttgaactgat aattaacttc
    36481 ctacgcaaga cctctccttt taattattta tctcgtcgtt ctgttccacg atgttgacat
    36541 actcgatgtg ttgcaactcg taaagccttt cggttatgct tttttcgttg gcagccgctt
    36601 ttctcattac ttttttggat acctcgtaaa tatattccac ggtaagctcg gtggtattct
    36661 ttactctgag attggctttt gccgaatagt agttgaatat aatggcttca atctgacgct
    36721 cggagtttcg tgctcctttt acgattacca gcatcctgtt gtcattcctt accttaccga
    36781 aagcaagaag cagcaggaac acaacggtac ttcctatagc ggctaccaga tagtctccgg
    36841 caccgcaaca gatgcctgcg ataattgccc agaaaatgta tgcagtgtct ctggaatcct
    36901 taatcgccgt acggtatctt acaatggata atgcacctac catacctaac gacaaggcta
    36961 tgttgtttcc tatgaccgtc attaccattg tcgtaagcag cgtcagcatc aaaagagata
    37021 cattaaattt cctgctgtat atagtacccg tgtgggtata catgtacgac agaaatatta
    37081 ccactcctac aacggcggct acggcaagat gcgacaatat ttcctgtacg gtcagagttt
    37141 ttccctgctc cagcaattcc aataaatatt tagtcataat ccttctcctt tttaaaatac
    37201 ataattaagg catatagtac gactgagtgc atatttactt accgataact ccgattgctc
    37261 cactctgtcc agcatgtctt taatatagga aagtaaaaaa ccgttgtatt ttacctccaa
    37321 aatcaccccg tcctgactga aaaccggata tgtgttaaga ttttcggaaa atatgtcaaa
    37381 gcaactctcg gtcgccctaa tattatggtc aaatgtaatc cttatactgt tctctttggc
    37441 aataaaagcc ttcctgttat attccactat ggctttgggt ctgtaaaaac gcatattcat
    37501 gaccgcatac aattctttgg caaaagggtc tttgtagccg agcaaaggtg tatatattcc
    37561 ctttgtaagc agtatggcat cttctcttgt catcttcaaa gaccttttca gctgctggga
    37621 gccctgtttc tgtttcatct caagaccggc aaactgatcg gtgggcttat atattctcaa
    37681 ccgtatcttt cttcttattt ccacgccgtc ttctttttcg acaaaatcat tatcccataa
    37741 agtatcaaaa tataaagaac gcaccatata gccgtgctct ccgttgttct cgtcctgagc
    37801 aaggtaggta ttaaaagcgg ccgaatattt tatgtaatcg gtctttgata tgatatactt
    37861 tttttcctgt cgcaaaacct gcttcatttt cgtctctttt tctctcgttt agattatatt
    37921 attcaaggaa taaaataaca acaggcttta cttccataaa gctgttttaa ttgatttaat
    37981 ttaacttaaa taaatattta acttaaaata ctattcttat tacctttttt ccagtctttt
    38041 caacaaaggt ttacatattg tttccagata tttggagccc agcaccatga ctatatcacc
    38101 gcttttcgca tgctcgaaaa gataatcctc tatcgcctga tatgacggca gatatatcgc
    38161 ttttgtaaca tttttgttta ttgcttgggc aagccttttc cccgacactt cactgtcggg
    38221 actttccctg tcattgtata tgtcggcgat tatagcgata tccgcaaggg caaagctttg
    38281 cacaaattcc tcaaacaaca cccttactct ggaaaaggta tgagcttcaa aagccgcata
    38341 aagtttatta tgtttcatcc tgcttgccgt ttccagcgtt accgctattt cggtgggatg
    38401 atgagcgtaa tcggaataca cctttattcc gtcggcttct cctataagat caaatcttct
    38461 tttggctccg tgtacaaatg acagatgagt acacacttcg gtaagatccg cccctaataa
    38521 ccccataacc gcaatgacgg ccagtgagtt cagtgcattg tgttgccctt cggctcccaa
    38581 ttgcacatga cagataaaat ttcctttgat atacagatca tatgacggct taccttgtgt
    38641 atctattgtc atattcatag cggtatagtc taaagataaa ttattcagac cgtagtatac
    38701 gacattacgg gaagcttttt ttgctacttc ccttgtagga atatcatcat aacaggctac
    38761 cagaaaaccg tctttatcta ttttgtcggc aaattccaaa aaagaccgct tcatattgtc
    38821 gaaggttttg aaccagtcca catggtcaaa gtcaatattc agtataactc cgatatacgg
    38881 tttaagcttt aagaaactgc cgttgaattc acaggcttcg gtcagaaagt aatcggagtt
    38941 gccgcttttt acgttaccgt tcagaaactc cgcttccccg ccgttgtgta tggtggcatc
    39001 atatcccatc ttttccaata agtatgctgc aagagtggtg gttgtggttt ttccgtgagc
    39061 tcccgatatg gctatcgagt acttgaattc ctccattatc ttacctaaaa gtaccgctct
    39121 gtccattaca ggtatctctt tagacactgc atatgcatat tcgcaattat ccggatgcac
    39181 cgccgcggta tataccacaa gatcgggttt tcccactttg gaggcatcat gtggtatata
    39241 tacatgaata cccatggata acagtttatc cattgtagcg gtatgctcta tgtcggagcc
    39301 ggttatcttt atgccttttt ccgacaataa tatggcaaga ccggacatgg aagtaccgtt
    39361 tatacctata aaatgcacat gtttgattcc ctgcaaaaat ccggctttca tgcttagctc
    39421 tccgcctcaa aagtctgtgt ggcaattata tcggcacccg taccgcatac gtccttaatt
    39481 atcacatctc cctgctgaac gggagcctgt attcttactt tatatatctc ttccattaca
    39541 tcgaaaatct tactcttact tacgggagcg ccggttttta cgggacagac cgtatatttt
    39601 gcgttatcta tcttaacgga ggaagtaatc attcttgtag gggaaagtat ctcggctctg
    39661 gcatattcat cgcccctttt gcaactgctg ccttccatgc ttataatgcc gttcttgtca
    39721 taattcactt gtatcgtaca tcccctggga catactatac agactatatc ttttttcatt
    39781 gctccacctc tatgaccaaa tcgccgaaac cgtttgtttc actgcccgta ttcaacaaat
    39841 ccctcatctt tatatccacc gattccattt ccgaaggaat gagacgcagc aactttcttc
    39901 gggtaagcac attatcaccg acagaagccc ttacatatac ttcgcggaat cgcgcccttg
    39961 aacggaaaaa tatctttatc ttttcctccg tatcctttaa atatatatgc tgcggcaaca
    40021 catatctcaa tccgtttccg gcaaccaccg ctattttttg tgcagacttt ttttctgtca
    40081 aagttttatc cagataggct tttgcactca tagccgctct ttgaccttcc atcgttacat
    40141 aatccacaag atcatgcaca tgcagcacat ttccgcatgc aaatactcct tcggtatcgg
    40201 tttccaggtc ctgatccaca aaaggacccc ccgtaacctg atacatgccc acgcccatct
    40261 gctcacttaa ttcgttttcc ggtataaggc cgaccgacaa cagcagcgta tcacattcca
    40321 catattgttc ggtgcctttt ataggtttca tgttgctgtc caccttggcg gtggtgactc
    40381 ccgttatcct gccttttccg tgtatcttga ccaccgtttc ggacaggtgt aaaggaatat
    40441 cgtaatcatc cagacactgc actatattcc tggtaagacc tccgggatat tccattatct
    40501 cataaacacc cataactttt atgccttcaa gagtcattct tcttgccatt atgagaccta
    40561 tatccccgct tcctaaaatt acaacctttt tacccggtaa tataccttcg atatttacaa
    40621 atcgctgtgc cgttcccgcg gtaaacacac ccgcacaatt ggaaccgggt atgcgcaatg
    40681 ctcctctcgg ccgctccctg catcccatag ccagtattac ggctttcgct tttataacca
    40741 tgaaaccgta ctcgggattg acggcataaa gagtcttatc cttttccgcc cctataacca
    40801 tggtatccaa cataacggta atatttgttt cttttatctt ttccgaaaat ctcaacgcat
    40861 attcgggacc ggtcagttct tccttgaaaa catgcagacc gaaaccgtta tgtatgcact
    40921 gattgagtat tccgcctaag gaattgtctc tttccagtat aactatatct tttaccccaa
    40981 gctcataggc ttgtaatgcc gcagccattc ccgccggtcc tccgcctatt atcgccaaat
    41041 taaattgctt aatcacgtga atgcctccct aatatgcacg aatcggcatc tgcttttgct
    41101 attttattca ccgcaattcc tgtatgctcg gaaagtatcc tcataacatg aggagcacag
    41161 aatcctccct gacatcttcc cattcccgct ctggtacgtc tctttacacc gtctaccgtt
    41221 cttgctccgc acggagcata aatggctgaa acaatctctc tttccgagac ggtctcgcac
    41281 ctgcatatta tcttcctgca caattcatcg gatttacagg ccgcttcttt ctcttcattg
    41341 gtcatttcgg aaaaaggctt gtcatgttta actttcatgg tatattgatt ttttgccttt
    41401 tccttcattt ccataagcga tttaaccata tcttcaacat ataccgctat ggcgggagcg
    41461 gcggtcagcc ccggagattc tataccggca acattgataa agttaccgca ctttttactt
    41521 tcttcaatga taaagtccga agtgtcggga gtggcgcgta tgcctgcaaa aaccgtaatg
    41581 gattttctta tgttaagtcg ggtgtaggtt tgcgaagcgg ttttgaatac ataatccaga
    41641 ttctcgctgt tggtacactt gtcatcttta tggtcggcaa cggtggcatc gggacctatc
    41701 ataagattcc cgtccacggt gggtgttact aaaattcctt tacctttttc ggtgggagtc
    41761 tggaacagta cggtatttat tctgctgcct tcggttttgt caaacagtat atattcacct
    41821 cttctgggaa taatattaaa agaatcatct cctgccatcc gacttatctc atccgcatgc
    41881 gctccagcac agtttattac tattttcgca gttatatcag ttctgtcctc ttcttttatc
    41941 ctgtccaatt tgctgattga gcagatatcg ccggtaattt ctgtggagat atgaaacaat
    42001 ccgtcttcct tttctatgcc tgtcacttcg gtttcccgaa gaaacttaac accgttcatt
    42061 acggcattat ccatagccgc tatattcagt ttatacggcg atactattcc ggcatttttt
    42121 gcatacaggg cttttgtgac cagcggattt acataaggtt ctcttttaag tataaagtct
    42181 ttgtccacaa tagccatatc ggtaacaccg tttttgatac cgctgtcata aagcttctgc
    42241 agaacctctt cgccctcttg gtcgaaagcc agtacaaaag agccggtctt cttaaacttt
    42301 acggagagat tgtcacacag cccgggcatc atattgcagc ccgagacatt gaattttgcc
    42361 ttttttgtac cgggtttggc atcatatccg gcatgtacta taccggaatt ggcacccgta
    42421 gcatccatcg atacatcgac atgtcgctct attaccgcaa tcttaacttt atacttggac
    42481 aattgtcttg cgatgaggct gccgactata ccggccccga taatacatat atcgtaatac
    42541 atacatcctc cgttatctat acaatacgcc gctctgtaaa atcattgtca gaatcaggga
    42601 aagcaccagt attgcggcca gtatgattgc tattgctttc ttcatgtgat ctcccttcct
    42661 tactgattaa ttttataccc gagccgtctt ttttgcaatg atacgctacc ttgcataatc
    42721 tttaagcaac tctctgaaat acacatagtt ctcaaaagga acttccggcg gaaccccgtg
    42781 gtcgcaggtg ggcagatagc ctccctgttt tttcataaaa ggtaagattc tgtcaatttc
    42841 cgcttttatg gcctctttgc tgtgagccag tattctctta tctataccgc cttttatacg
    42901 tatgtcggga taatcttttc ttatctgcac cacatcgcat cccgaggcca cttccatggg
    42961 agacatatag ttcataccta tctctttata caacggaatg accggtctgc agtcaccgtc
    43021 ggtggcaaca tgaattataa ggtcataggt tttgggttgt ttgtacgcac actccttatc
    43081 actcattgca agtcgcgcat ttctgctcct taatccctgt ataagttctt tgtaataggg
    43141 aaacagaaag gtgcgtatct gctcgggaga tattaacggg ccgtgattgt agcatatgtc
    43201 ttcatccagc agcagttcat ctattcttac atggtcctga tattttgccg ttacataatc
    43261 ggataattcc agccactttt ccatgcaggt atgaagcagt ccgggatcgt cataaatcat
    43321 atacagtatt tcttcaccgc caacgagcga acgcagatac ataaaagcac ccacaatata
    43381 catgaccgaa tgataccctt tctctgccaa cggtacggag tcggatataa actttttcag
    43441 gtcctcgttt ctttgggcat ttgcataatc cagtctccac ttgatttcct cccatgtggc
    43501 catatcctta acgggaaaat ccagatattg gggcataaac ccgtctcttc ttcctttgaa
    43561 atatttgacc gtccttccgg ctttgtctat tgctatttca tattgctcat cggaatgaat
    43621 aacctcttct ttgaatcggg gaacaaacgg ggctacagtt ccggcaagac cgtgaaggta
    43681 atagtttgta agtcgttcaa aaccgaagag tgctaccatg tcttcacctt ctttaatata
    43741 tccttggctt atccactgct ctttgctgta ataaccgaac tcttccaacg gtatgggagc
    43801 atcgggtttc atggaataga aatctgcggt cattttttta agttgctcaa aatctctcat
    43861 actgtataaa tcaacctttc ctgtattctc ttaccgtttc aatcatattt atatagtttt
    43921 cggtaggcac ataatcggga acggaattac cggtacctat tgctatacct ttttcgttac
    43981 ccaccgagta aagcagatcg gtaatatatt ccttcaactg ctgtttatcc gttctgcaca
    44041 atacatcggc atccacccct ccgaaattac ctatacggtc tccgtaccgc tccacccaca
    44101 cggaaaacgg tgctatctgg tcttcattgg aatgcttggc atttatcttt gccacattga
    44161 taatatcctc catcacattg aaaatattac cgcaggaatg taaaagaaac ggcaagccgt
    44221 agctgtggga aagtcgtaca atatcggcat agcccggaac aatatacctc tttatatctt
    44281 cggctgaaag catggtgttg gatttatatc ccaaatcgtc gcccattctt gctacggcac
    44341 acatatcggc atatttatcc aggaaccctt gccatatatt aacaaacagt tttctcattg
    44401 ccaaaaatat atcggcatac agttcggggt cctcatagga cattatgcaa agattcatgt
    44461 atcccgtcag gtcctgaaca cattcgaata taccgtaacc gggaccgcct atcattttca
    44521 tacccggagg gagcgattgt ttcaaagcca tgtagaattc atccgccctt ttaagaaata
    44581 actccggtat ttcctcccac ggataattga taaaatcctc tcttgatgtt atggcgggag
    44641 taatatgttt atccagagct ccgccgcccg gaaaagactc tcttatcgcc acttcaaaag
    44701 aaacggtatc atatcccatg gttttaaaga aaaggcaata ccgattaaag aactcgcact
    44761 tatcggaaaa atcgccgtca atgagagctg caaacttttt attgagtatc ttttccatgt
    44821 ggtcggtact gatactgtgt tcatacatcg gaagcctttt cgccttttta ttaaatgccg
    44881 catcctgtat gtttctgtaa tccggtgtga attccataac cgtcctttaa tacttatctt
    44941 ttttctaatt atatcatcaa ataccgcttt aaaatataac tatcacctat acatatattc
    45001 aaagaataca atcactacat ggtataatga gatgaaacta taaattcaag aggtacatca
    45061 tgacaacact gaaaatgacc gtaaacacaa ccgttaaaaa aggacacata agtcccctta
    45121 tatacggaag ctttgccgag catctgggaa gatgcatata tgacggcata tatgtaggag
    45181 acaattccga tatacccgat accgacggta taaggaatga tatagtggag gctctgaagg
    45241 caattaaact gccggtgttg agatggccgg gaggatgctt tgccgatgaa tatcactgga
    45301 gagacggtat aggagataag tcaaaaagaa agaagatgat taatacccat tggggtatgg
    45361 tcatagatga taacagtttc ggtactcatg aattcttaag attgtgcgaa cttatagggt
    45421 gtgagccgta tattgccggc aatgtaggca gcggtaccgt tcgtgaaatg gccgaatggg
    45481 tggaatactt aaactgtgcc gcacctacat cggttgtgct ggaaagagcg gcaaacggac
    45541 gcattgagcc gtggaaagta aaatacttcg gaataggaaa tgaaaactgg gcatgcggag
    45601 gcaatatgag acccgagtac tatgcggatc tttacagacg atatgcggtc tactgcagaa
    45661 actacggttc cggcaggtta tataaaatag catgcggacc cagttctcat gattataact
    45721 ggaccgacaa gctaatggaa agcataaccg ccaccaaaga ctgttcggct atttctctgc
    45781 atcactattg cttaatgacc aatgtaagag caaccgagtt atcgtcaacc gattatgcaa
    45841 aaacaattgc caaagcacag gcaatggaca aaataataga gaaacactgg gaaataatgg
    45901 gcaaatacga tcccgatcgc agaatcaagc tggttgtgga tgaatggggc acctggtaca
    45961 ggtccgaacc gggaacccat ccgagcttcc tgcatcagca gaacaccatg accgatgcca
    46021 tagtagcagc cggaacgctg gatatattca acaacaagtg caatattgta gacatggcca
    46081 atatcgccca gacggtaaat gttctgcagt cggtaattct gacaagcggc aaacagctta
    46141 ttaaaacacc cacttaccat gtgttcgata tatacaagga acaccagaac aatatgcttc
    46201 tggaaagcag ctgtgagaac agaatattta acagtgacga agaaatttat gccgtatctc
    46261 attcggcatc gttaaaaccc gacggttcgg tattccttac actttccaat gccgatccgg
    46321 ataaagcata cgaaacggat attaatctca taggaaacaa ctatgacaat atatccgccg
    46381 aaatactggt ttcggacaat tatctttccc acaacacctt tacggagccg gaaaagataa
    46441 agaagcaaac atatacacag tacaaactga agaataacag attgctgtta acgcttcccg
    46501 catgctcggt tgtaggtata gctttgaaat agctttatac tgccggaaca actgcctcct
    46561 tcttgcttat atccccttat tatggtagat tgtcggaagg aaaagaaaca aacaatgccg
    46621 gacatgttcg gatattaata ttaaaaacta aaagggagta aaaatggaca agttacaaag
    46681 cctttatgat gaaattaacg gaaacacgga aagcccggtg gcatatatgc ccaagacccc
    46741 tataaccagc agattcgtat ctccgtggga tacttgggga tggtatacgc tgaaaagcaa
    46801 tttccgaaag ggagttgcac tttattccaa cagtgacgat tatgtaaaaa acatagatga
    46861 ctgttacgca ggtgccgact atatccaaac cttcaattcc aaagccgtca acttaaatga
    46921 ccacccggag ctggatttct ttgtggaaac cgatgcgagt gttacggttg caatggaaga
    46981 aggttgcatg ccggaatggc tgaaagactg gacaaataca aagaaaagca tgacatcagg
    47041 taaaggtata aaatatcttc tttatacaaa ggaattccca aaaggtgcac atgttcatgt
    47101 accgggcttt gaaaccgacc acaaccatta catagttatt atccttcctt tatccaaccg
    47161 ggagaagtta agtaaaacag acaaaataca ttacccgaac acacagttac aacctcacaa
    47221 aacccgtctt tatcaaagct atatagtgga ggttttcaat gacaagaatg acggtatatt
    47281 tgtaagtaac gactaccgat ccttcggttg ctgccatatt aaaacagatg ataaagacag
    47341 gaaaaacaaa taccttgcgc tggaaacaac cgacaaatgt gataaagcat atgtaaaaaa
    47401 gagtgtcgga ataaacattg aataccctat cgtttttgag tgcaaactca acatttccaa
    47461 agattccgca atgcaggcat tacttacggg aagtaatgaa aaaagcatcg gagcgatatt
    47521 caaaaaagac ggttttatct atgacgccga gggaaaaata aaggtatgtg cttttacaaa
    47581 gaataccgat gtttgtttga aaataaaggc ggatacgcaa agtaaaacat atgaaatatg
    47641 ggtaaaccac gtcaaacagg caaagaatat accgcttgac atggaagaca tacagcatat
    47701 gtgtttccat gtacagagtg ataagagttt atcttatgca tacgtggaca atatatatct
    47761 ttatgatgat acggaaatat atgcggttaa cgaaaccttt gaaaccgata cccttaacaa
    47821 ctggacatca aatggaaaac ttgcaatcaa accctatccg tttgacaaag acagaagctt
    47881 aaccttaacg ggagcatcct atgcaacata tgcattttgt cctgtagacg acatagtgtc
    47941 gatagaaacc aaggttaaag tagccgatga atcttttact cttgctcccg agattgcaga
    48001 taaatgcggc aatgtagccg ttaaagttgc tttgtataaa aacaacctgt atgcctccga
    48061 cggagaagtc tggaagagaa tatatgaagg acttacaccg tggatgtatt atcctcacaa
    48121 taactggttt aatataaaag tgacggcgga tattaagaag aacacatacg acctttatgt
    48181 ggacggagca aaaagagcgg tcggattcag gtttattaat aagacaaata atctcggaca
    48241 gttagccttt acttgtgaga aatcttcaaa agtatatatc aaccgcataa gaatatatga
    48301 ctgtgcggac ttttccagag gagtactgcc caatgcaaaa gtatttgatg taaagagcgc
    48361 tccgtataac gctaaaggtg acggaaaaac actggaaacg gccaagatac aaaaagccat
    48421 cgatgacgcg gcatataccg gaggaacggt gtacattcat gacggtactt tctttaccgg
    48481 cggtctcata cttagaccgg acatgacgct gtttgtcgac agaagtgcaa caataatagg
    48541 tacacaggac cacagccaat ataaattggt atcaccggga atttctctct gtgcggtaag
    48601 acaactggga aggggtcttt tatacggtga aaacatatct aatataagaa ttaccggcgg
    48661 aggaacttta gacggtaacg gtacttacag atacaagatg aatgatcctt tgcaagacag
    48721 agaggcggat gcaagaccgg atatagttta catatcctat tccaatgata ttgttgtgga
    48781 aaatgtggac atgaaaagct ccgcattctg gacggttgtc ccgctttctt cggggaatat
    48841 aactataagg aatctgaatc tggactgcat gaacacgccc aaccgtgacg gcatagatcc
    48901 tgtggactgc catgatatga ccatttataa ctgtaatata atggcgggag atgacggact
    48961 gtgctttaag agttcggata atgtaggctg ctataacata gatgcatatg acatgatgat
    49021 acagagcctt gcaagcggta ttaagttcgg aaccgataca tattactgtc ttaaaaacgc
    49081 aaggataaga gactgtgcca taaagaatgt caacagatgc ggagtgtcac tggaatcggt
    49141 agacggagcc gcagtggaaa atgtaatctt tgagcgtttg gatatgaccg atgtgggagc
    49201 cccgttgtat atatccacag gtatcagaaa cagacttccc agaggtaatc aaccggtaag
    49261 aagaagctac atgaagaatg taaccttcaa agacattcgc tttgaacaac cttatccgtt
    49321 cagttttgaa agagaaattc gcgagaacat ggttataggt cagtccaagg acaacttaat
    49381 tgaaaatgta aacttcatta acttcgacct taagcttccg ggcggtgtaa aaaccctgcc
    49441 taaacctccc gtagtcatta acgacaagta tcccgaatat gacagacacg gattatcttc
    49501 aggttatgcc tttacaataa aatatgccaa gaatgttaag ttcaaaaatt taaaagtaac
    49561 acttgaaaat gaagatatac gggatgaagt ggcatatttt gattatgagg aatagataat
    49621 atctaaatta gccatatagg aactataagg ttatctttgt caaatgcacc gagtttctcg
    49681 gcaagacaaa gtatcgcacc tgttcccctt ttaagaggtg acttgtcaat aattgaaaaa
    49741 cttttaacca gccgcttgtc cggcatggct gcctttttta tttccacggg acacaatttt
    49801 ccgtcacctt cgataatgag gtcaatttcc ttactatccc ggtctcggta gtagtatata
    49861 aaaggttcac gcccgcagtt ttggtaactc ttaataattt ccgctattgt atagttttcc
    49921 aacaatgccc cgctcatagc accgttcata gctgtttcgg agctggacca tttggtaaga
    49981 tagcagacta acccagtatc gaagaaatag agctttggcg tttttatagt acgctttaga
    50041 acattgtttg aatacgtatg taaatagaaa ataataccga gggtttctaa aatattaagc
    50101 cagttcttag ccgtatattg gtctatctcg gcatcgtccg caattccttt gtagttaaca
    50161 agttgtgccg ttcttgccgc aactgccgta ataaaccgca tgaatttcat tgcatctatc
    50221 gcttcggaaa gatcacttac atcacgttcc aaataggttt tgatatatcc ggaatataca
    50281 accgagcggt ctttatattt gccgcttacc aaagcaggca ttccgccttt ccatatgcgc
    50341 tcaaacaatt ccggagcgga caccgatgca actgctttct gtcgttctaa taatgtatca
    50401 aaatcagtgg taaaaggaac cgttacacct ccggttatct cactttgaga caacggtagc
    50461 atctgtaaaa gtgctacacg tcctgccaaa gattcctgca cacctctcat cagtttgaat
    50521 atctgtgatc ccgtcatcca gaagtcaccg ggattgcaac ctttatcaat cagtattttt
    50581 acatacggaa acaactccgg agcatactga acctcatcta taaatatcgg aggtttatga
    50641 agctgaaaaa acattgcagg atcggttttg gcaagcatac gatcgctaat atcatcaaga
    50701 gtaacataag tacggttttc gggctcactc ttcatcaatt ctttaagcat ggtggttttc
    50761 ccgacctgtc tcggacctgt aatgaggata gcgggaaatt gctcgcttaa tcgcagcacc
    50821 acttcttcca tatgtctttt tatatagttc atttcatcat ctccgtttcg gctaaaatat
    50881 ggctatattc atattatagc cgaaaaacta aaatgtcaat agcgtcaacc ttaacttcca
    50941 gcaattttga tagaggaaag tattacctga agttaaggat gtaaagttga tttttcttga
    51001 tgccgtaata gcaaatcccg atcgtcatac aaacaatttc gggcttttaa gagatataaa
    51061 taccggcaca ataatcggtc ttgctcccat attcgatcac aacatggctg ttacagcacg
    51121 cggttatccg ggaaacccca aagcaaccga cctgctgata tctcttttca atgaccttat
    51181 gaagaaatat cctgaataca ctactcacat accatccgtt accgaacaga cggtaatcaa
    51241 cattttagat aagataaaca tgcgtgttaa gagacaggta ataatagacc ttgttatggg
    51301 gcggtatggg tttattgagc agaataatat agattagtag ttagattaaa tatgaaatat
    51361 atcattacgc ccctgcactt gcggtacgtg caagttgaag tacagttttt tgattataat
    51421 atttatctgc aagtgcaagt aaaacatcgt acctcaaatt ctgtttttta atatgattgg
    51481 caacaacact atacggttga tcttcatcaa gcggagcact gtccatcata tctaagacat
    51541 caagtatttg aagatacatt ttattatctg ccgttacctt tatcttagga accttaatag
    51601 ccacacctaa ctttttatca taacgcatac aatctcttgc aacatttgtt gccagcacag
    51661 gatgcttagg catttgtgag gttaaaccta atctatgaag cagtgcagga cctgtttcat
    51721 acccgttatt attcgccaaa tacttgtcag atataagttt gttcttatta attccttttt
    51781 caccaaaaac cgtttcggtt gtacgataaa agatgccttt ttgatagctt cttaagtcgg
    51841 gaattgttct gttttcaata atacgtttca tggcaacagc tactgccgct gatgcgtctt
    51901 tgtaatttaa tttaaaagca tccttaacag attctgataa ctgctttgaa taaataggtt
    51961 ctccgggctg tattttacga ataatcttac acaagtattc aatatatgtc ataaaatgct
    52021 cctttcatgt gatatattat atacgtattt agcaattata taccgttatt attatatcat
    52081 ctgggaagaa tgtgtcaata gcctaattgg atagataaaa ataagtttct atgcacaatc
    52141 ttgcttttcg gctttctttc gaatgttaaa aaacgtataa gtctccctat acgtttcttg
    52201 ttggttattc ttttgttttt cccgctatat attaccgcta agtttaagaa tgagcaggat
    52261 tattgcaatt actataccta ttgctaatgc aacacgtgcc ggtgaaacag gtgaacgtcc
    52321 gccgaccttt cctgttcttc cgttgaccat gaaccggtaa atcttttctt tatacttgaa
    52381 tgatgaaagc catatcggca acagcagata cttgtacttt atatcattgt actttatctt
    52441 catatccagt tttcttacat cgtccgcatc gttttcttct tcaattttat cggttatctc
    52501 actatgaagt atactttcta tatcctcctt agcaatattc catcctttct gaagacctac
    52561 ggagtatctt tcggatatga atcccgcaac atattccggc ttgtaaggta tgctgttttc
    52621 cgtgtcaaac ggttttatca tgttaagagt tccctgatca tatctttccg ttgccggaac
    52681 gggatactcg tcaatgaatt tttcgtatgt tccgcgtgtc ggataccatt ccataacctt
    52741 cttggtttcg ccttcgtcgt cctttacttc tttttctttt ccgtactcgg cagtgtactt
    52801 tacatccgct tcagtatcaa atgtccagta tggcagataa acacccttga aagactccgg
    52861 tttggcacct tcttttgcag ctttgggaca gaacagtttt ctcttaagcc agttatggaa
    52921 gtttgcccct gcttctttat cggttacggt gaaagggcac actccgtcgg gagccaatga
    52981 atccgctccc ttttcctcgg tcacctgatt tgaaccgcaa tacggacata catcggatat
    53041 ctgaagagca tcatataccg atacagcagc acaggattta catactactg tctttttctc
    53101 agcaccccag ttgaagtttc cttttttctc ctcggcaaga aagtcctttt cttctacttt
    53161 tctgtcttca gcttctttaa cagatatttt tctcgtataa ccgcaatacg gacatgtaag
    53221 acctcccgtt ttgggattaa agtccatggt accgccacat gaaggacatt tttggtccgt
    53281 ttcccgtttt gtatctactc ttttatcttc atacatttta tattacctat tttacctcat
    53341 cggcatcatt aaatgcgtca ccgcattccg gacagaactt aggaggggta gcacctgcag
    53401 gtaaatccca tccgcattta tcgcatttat atgttttagc tccggcaggc ttgggttttc
    53461 cgcactctcc acagaactta cctttattaa cagtaccgca ttcacatgtc caaccggctt
    53521 gttcaacagg cttgggtttc ccgcattctc cgcagaattt tcctttattt acggttccgc
    53581 acgagcatgt ccagctgttg gggtccggag cattcggtcc ttgctgattt ccacccatag
    53641 catataagtt tgaagcattc attccgcctg cctggcctgc taaattcatt cctgcaaatg
    53701 ccataacggg acctgcacct tcattctgtg ccgcatgaac cattgcttcg gactgagctc
    53761 cgaccaatgt tgctgccgcc atagtaggat ctctcataac agcgcttctc tgcagcttct
    53821 tgattaattc ttcgtcctct tccgatgcct ttaatgaact gataccgaaa gtacctattg
    53881 caattcctct tgtctgagtc catttttccg acagcacctg attaagagca tctgcaagtt
    53941 ccattgtgtg tccgggtaaa gcactgtacc ttatacccat ttcggaaatc tttgcaaatg
    54001 ccggttgcag tgcggtcata agctcggttt ttaactgtcc gtctatgact tcacgtctgt
    54061 attcatccac tacattaccc gttacattgg tatagaagag taccgggtca actatcttgt
    54121 atgaatattc tccgtggcat ctgattgcta tatcaatatc caaccctatg tttttgtcta
    54181 taactctgaa aggtacagca ttaggtgtac cgtatttgtt accgacaatc tctttcatat
    54241 tgaagaaata tactctctga tctttccccg gatctgcgcc catggtaaaa cgctttccta
    54301 cttctttaaa gctgtttaaa agaccttctc cgaagttgcc gtacaacata ctcggcgatg
    54361 tggacgtgtc atatatgaat tctcccggtt ctccggaaaa atccactacc tgaccctgat
    54421 ccactatcat catgaactgt ccttcattaa cagccactat ggatccgttg gagattatat
    54481 tatcctctcc cttaacatta acacctcttt tgcctgttct cttctgtcct ttttttacaa
    54541 gaacatcggc atccattgac tcgcagtaga aaaattcacg ccattgatca gcaagtactc
    54601 cacctgcggc acctacaaca gctcttatta atcccataat tgttcctcct gatatgtatt
    54661 tgatagtaaa aatgttcaat aacagtataa cattgcaaac aaagcgattc aataagggat
    54721 ttgttttgta tataattata cgtaagccaa aagagcgttt tgaggcctaa attttcgacc
    54781 tcaaaacacc ccttcgtttt tgtagttgaa cctaaagtca ttcaaaaccg ttttaccacc
    54841 atataggatt acaccattct caaacgaaag cacagtaagt ctttgttttt actgtgcttg
    54901 catgtataat tttaatatgt atggaggcat aaaatgaaaa aaacaatctt actattcctg
    54961 ttggtttgta tcattgtttt agccggttgt cagaagcagc cgataaaaac cgatcagcca
    55021 acaacatcta tacccagtca gacacagaca ccaactccca gttcggaagt cacacagact
    55081 gaagagccgc ctgaaaagtt taacctttat gaggatacaa agggaatggc tgttgttaaa
    55141 attcaggacg gagaggcaag tatagcaatt aatccgaaga attggtcgga tgttccggag
    55201 aatcttgata gagataaaca aactgccttc tgtaagataa acggcttagc aggaaagata
    55261 tcaactgctt gtgtgggtga aatatccgat ttgtcacaaa ctttcggggg agatggaccc
    55321 tttactacac cgattgcggt ctttctaatg gaggacggca gcttggaatg ggtaacggta
    55381 aatccctggt ggtatgtatc cggtgcagaa cagggatacg ataaggtagc tcacagttgg
    55441 ggaaaaattc cctggatgga gaatataaca tcgctttcct tcggttcgga aaatgatggt
    55501 gacggcagca gaactaccat ttatgccaca gacaaagacg gtttgcgcta taatcttaaa
    55561 tacccttgta actttcaatc tcttttgcac acaacatggc gatataccat tgaccctgag
    55621 gacaaagatg ctttgtatct gacttttgag gattttgaaa gagtggattt ggatctcaga
    55681 tggggagccc agccgctgga aaactttttc ggtacttata agctttactt agccgaggac
    55741 ggaaaacaag gaaagcaagc aggctgtatt gagtttgatt ttactttagg atggtggatt
    55801 tatgaaggtg aaggcgagat agatcgtccg acagcaatca aaggaacttt ctttacacaa
    55861 tttgcaagag attggagtct ttccttaacc ctttggccgg cagacggaga tcctttgtat
    55921 gcaacacgcg aaaaaacacc ggatgaatat cgtttcttgt ctgcagatat gctcgaagca
    55981 attcccgaat atacaatgga cgatgatgaa ctggtaatct accttatgta tatagttccg
    56041 gatgtaaacg cgatgataaa ggaaagcgga atgtccgcct tggtaaccgg tgagactacg
    56101 gaacttgcag gtggtgtagt atgccgtaac atctggctcg gtacaaagaa ggatgattct
    56161 tttacccgcg aattgcttta tggtgtcagt cccgacggtt cggtttacag atatgatgca
    56221 ttgagcgaca gctggacaat gttacattaa tgactataac aaattttata ctacaaggtc
    56281 cttacataca gctatacaat acgaagtagt cgattccgta tagattataa aaggaaagtg
    56341 tcagagtgcc ataatatgga tggcattctg tttttctgtc tttttaaaaa gcaccgatta
    56401 catcgatgct ctttggtcgg agtgacaaga tttgaacttg cgacttcttg gtcccgaacc
    56461 aagcgcgcta ccaaactgcg ctacaccccg tgcttagata ttataacccg tcttttgtgt
    56521 tctgtcatct gctatttttt aatattcttt atgtcatcat atcggttata tccacatcat
    56581 cgtctttgct ttcttttctg acaagcatat ttaagaaatt cgttataggt ttcctgaata
    56641 ttattacaag tcctataaga acaagcatta tgacaataac caatatcatc tgtcctgttg
    56701 tccatttgag cacaccgtct cctaaaaatg ccaaggatag agttgagggt attcttgcta
    56761 caagcgctag gaataagaat atgctaaagg gaagcatcac tatcccggca acataacata
    56821 aaaggtcgtc aggtaatccc ggaataagga atgctaaaag gaagaatatg ggaccgtttc
    56881 ttttgacaaa atccacaact ttttttgtct ttcctcctcc cgccaacttt tcggtaagag
    56941 gctctccaaa cgctctcccc aaccaataaa cggttattga tgccaatact gttgccgtat
    57001 ggttgacggc aaaacctccc caggtaccga acagcatacc gcctacaaaa tatgttatct
    57061 gacccggtat gggagcaatt acaacctgta agtaggaaag cagaaagaac agagcgggac
    57121 cccatttgcc tttactgtca atgtattctt taaactgttc gccatttaac tggaataagt
    57181 cccacagttg ttttcctttt tcggtcagaa taaagataag aaaggctaaa ataatagcag
    57241 atgctatgat aagctttata ttccttcttc ttatcttttt ctttttatct tcactcatct
    57301 ttttattgat gcaatcattt ctttaagcat gtttccgtat tttatgtatg attggaaaag
    57361 ctcttcatca ccggcgggtt tctctcctgt atgaacaatg gcagctacat gcggttcaat
    57421 ggatataact ccgtcatact tgtcctcatt gattagaata tccagtattt ctcttacttt
    57481 tccttcacct tctcctacgt agcggaattg attttccaga ccgggtcttg caataagtgc
    57541 atctttgata tgtacataat ctatattccc cctcagtcct tgaaaaaact tcataggctt
    57601 atcataaccg tgagccacta catttcccgt gtcgtaaagg agtacaaagt tgggactgtt
    57661 tacttcctgt ttaagctcca gcatattttc cggagtttct cctccccaac ccgtgcagtt
    57721 ttcgtgagcc agtataacat ccagatcctc ggccatcttt gcaagttctt tacatcttct
    57781 gattacttct ttctttgtgt attttttatc gtcttcatcg ggaatccagg acattatcct
    57841 tatatacctg acattgagca tcttcatcct ctttgcagat attttcagat cctctttgtc
    57901 aatattgaaa tcccctctta tgctcctgct ccagttacct atagctgaag aaaatgcagt
    57961 tactttaaag ttgtgttctt caataatttt ccttacatta tcaaactcct gatcactgaa
    58021 gttggtggtg gaaggattaa caccgttatg atttcttaat tccaaggtat tccaccctaa
    58081 ttttttgtgc gcttcaatct gcgtggtaat atgtttgccc gcttcatcgc tgattcccgt
    58141 aataatcatt tcttctcctc tttattgttt tctcgtattt cttctaaaac cccgtcactc
    58201 cagagtttct cgaggttata cctgcttctg gtttcttttt caaatatatt taccactaca
    58261 tccatacagt ctataagtat ccatttagat tggttatacc cttccacagg catggaaacc
    58321 gtcattccca acgcttccgc cttctcttgc acatcatcgg ccatggattt acgatgacgg
    58381 tcggaagttc ccgtgcatat tacaaaatag tctgtcagtg tagttacatc tcttacctct
    58441 atggatatca aatcctttgc ttttttttct ttcagtacct caacaatttc attcttaaaa
    58501 ttttcagtat ccaatattac ctccttgtat gttttgatta tatcataaag agtaagttat
    58561 aaatgagaaa tacggaaaat catgaataat cgttaaatcc ggggaaaagt tgcatataat
    58621 tgctaaaagc attattatgc ttagagtatg aggaatggag gaaaactgta ttaacgaatt
    58681 actgaaagta acattgatat ttatagggat atttataatt cagtcgatgg tatactgggg
    58741 cactcagttt cttgttaaaa aacctcatat gctgggaagc aaactggatg ataagatacc
    58801 gtttataccg gttttcattt tcccgtatgt tatctggttt gttcttttgt tcacaataca
    58861 gtttgttcta tacttttatt ccctgcaaac atatgctctg tatgtaatgt ctttacttat
    58921 agtggatata acgagcggaa taatatatct ggtatatccc acaactttca tcagacccga
    58981 acccgaagga gattcttttt ccgttaaagt agtaagattc atttactcgc atgataagaa
    59041 aatactgagc tgtctgcctt ctttgcactg ttctctatcc acgcttttta tattatcggc
    59101 actttatgca accggtcttc cgttattttt aaaactgata attattatat tttcactact
    59161 cgttatagta tcaacggtat ttgtaaaaca gcatgtaatt atcgatgtta ttaccgccat
    59221 acctctggct gttataagct ggtatatagc tatggcaata ggtgctgata cgtttcttac
    59281 cttcttcaat tttttacagt aattttatag ttacattaca tttaatgtat agaatatatc
    59341 tgagaggtgt taatcatgaa aacggcaata atatattact catacagcgg taaaacaaaa
    59401 aagtactgcg aaaatatggc agcggaactt tccgccgata tcctggaagt gcagacagtc
    59461 aaaagaaagt gttttttatc aaatcttttt accgaatgcc ctaaggcact acaacagcaa
    59521 cctacggata ttttgcctat tgatttggat ttgtcgggtt atgaaaaaat tatactggca
    59581 gcgcctatgt gggcaggctt ccccgcaccc cctttcaata acatggtagc catgttacct
    59641 aaaggcaagc aagtggaaat atatattgtt tccgcaagcg gtgaaacaga tccgataaaa
    59701 aaagaaaagg taatagtatt gactgaagaa acaggttcaa aagtcatcaa gtataccgat
    59761 atatgcggtg cggatataaa ataggttatt ttgtttcgtt taaaaaccgt actttattaa
    59821 ttttagcgat atccgatgca atagtctcgg cttccgttac ggtatctttg ggaacgggta
    59881 tccggcactg gagcttttga ggtccgaatc gctgccatat tgtaaaatta accgttatgg
    59941 tatagttgtc ttcacggtct ttggcaaact gtacagatgt gattcttgtt aaaggtgctt
    60001 tccactgaaa atactcaccc ataatccatg caccgaacgg acttattaac acttccggag
    60061 cagcctcttt catcttgtta taccgagccc tgggtattaa aatagccgct aaagatatca
    60121 atatctgaat acccagcaat actacaatga agaatatggc atccttactg ttgctgaaac
    60181 cgaatattgc aaacaatcct cctattatca caacaaacgc aattattacc agcagcatta
    60241 ctttgttgga ggtttttctt tgcaggaact cttccgccgc tctttgcagt ctttcattct
    60301 cttcaaatct ccaatgggct aataaacccg ttcctttcag catgctgtcc agatttctcg
    60361 cctgccggat aaacataaaa aagcatataa agaaagtaag gaataatata gctccgacat
    60421 aaatcatcca gtagtaattc atttcgccta taaccgctcc cgctacaatc atcgctattc
    60481 cgataagcat aaagattata gacataatca cccatctctt tgcattattc tgtgcccaca
    60541 tatcttacct cccgattatt ttaagaagat tttccgaaat atgattaaat cccgaatctg
    60601 cgggatgaac tgtatggtcc tgatgatatt tggtgtcatg cggcataaga gccaagccgt
    60661 ttacgggaat aaaaccgtat ttctttgcta atgtcgttaa ttctttttga tactcatcaa
    60721 acatttcctt aaatgattgg tcttcatcgt atcttaaaca ccatatcgga gtaataatat
    60781 atacttttgc atgcttgtag atattcttaa gtttatacaa atacttgtcg gctgttttca
    60841 gtacattttc cagttccgct atatctttta attgatagat atcgttgatg ccgtatgcaa
    60901 taaaaatact gtcgggatca tatttgtcct gttcatccaa agaatccaca tcaaacttcg
    60961 ccgaaccgac acccctgttc agactctgtg cattcaaggc tcttgcaacc tgtgtgggaa
    61021 aagccaacga aggactcaaa gctaccatgc cctgggtaat ggaatctccc agaaaaagaa
    61081 gtttttgaga gggttgctct ttaatgcatt gaaaattacc tatatccaaa tcatacaccc
    61141 ttattctgct cagattaggc acataaacgg tcactctctt tatcgctttt tccgttaacg
    61201 gataagttaa agtagactgc tttgcatcca atgcttcttc tatgtgtctt ttaaatatac
    61261 cgttttcgta aaaatcaaat cccacatatc ttctggaaaa atggtcggct ctgtaggaaa
    61321 aggttataca ctcggcatcg gtataaaaat ccatacatat accggctgta cacagcgacc
    61381 ggtcttccgt tgccttggaa aggtttttat agtattccag ctgcttatcg gtaaaccgaa
    61441 cgggaaaata tccttcctcg gtttttataa ccttgtatgc gtttttaaaa atacccgtat
    61501 acattaatct ttaatccttt gctcttggaa atactccaaa gcggcagctg ttacgggata
    61561 gagaacttct cctttggaaa gtacatgata taccgtccct tcaaggctca gttccaccgc
    61621 ctcatcaagt ttattgtaca ttaatttgcg tattttagtc acgtttttgt atgttcttcc
    61681 tttttccgca gcatcggcaa taaatactat tttttcggta agtcccatcc ccgcccgtcc
    61741 gaacgagtga taataaatgg cattaagaat ctcttcatcc tctatgttat attctttttt
    61801 tgctattacc gcggcacagg gaccgtgcag tatattgccg gtatgatatt gcttttcaat
    61861 aaaaataccg gcagcttcaa gtattgctgc catatcttct tctttcatgt ctttggcaat
    61921 atcatgcaca agtgccgccg catatacttt ttcgctgtcc gccccgtgta atcttgcaag
    61981 atgatctgcc aattcgacga cattctttac atgctcatat ctttcggggc tatacctttt
    62041 tactatatcc agccttgcct cttcacttat atatcccatt ttcttttata tacctgtaca
    62101 cttttttgtc cagcatccac tctatcgagt tgtcctgctc cgctatccct ttacgtattg
    62161 cggtagagga tatttccgat atgcatctta tgtcgaatat aatgtcggca ccgtaatctt
    62221 ttttgaatat ttcaatctgt tcggttatct gctcttcggt aaagcccgat cttccgaata
    62281 ccaaaaacgt gcagagtttg aacacttcct catattctct ccatgtctta agctgaaaca
    62341 gggtgtccgc tcctatgata tatattatct tttcaaaaat cggatattct ttttttacct
    62401 gtttcaatgt atctatcgtg tatgtggttg tcgctctttt aagttccata tctgaagcta
    62461 tgagtttttc ataaccgtcc aatgccagac acagcatcgt atatcggata ttgtcatcgg
    62521 ttataccggc attatcttta tgaggcggct ttgtacaggg tgtcaagagc acatactcac
    62581 aataatccag tgcgtatttc gccatcagga tgtgagcctt atgtaccgga tcaaatgttg
    62641 ctcctaagat tcccagtttc attttctgcc tatatctttc ctgtaatgca tgtctttaaa
    62701 gcatacttta ttgatattat tgtatactct tgcgaccgct tgttcaacag tatctccttt
    62761 tgcagttaat gaaaggactc ttcctccgtt ggtaacatat tccccttctt tcatggctgt
    62821 tcccatatga taaacggtta tttcctgctt ttgtatttct tccaatcccg atatcggata
    62881 acctttggta tattctttgg gatatccgcc gcttgcaaga accaccgtgg caccggcact
    62941 gttctcccat tgaatgtcag tcttgtctaa cctttcatct gttatcgcat tgaatatatc
    63001 aagaaggtcg gttttcagta acggcagaag tacttgtgtt tcggggtctc cgaacctggc
    63061 attatactca attaccttgg gtccgtcttt tgtaagcatc atctggaaat atattacgcc
    63121 tttgaagttg cgaccttctt tcttcatggc tcttattgtc ggataaaata tgttgttaat
    63181 gcagtattct tctatctctt ttgtgtaatg cttgctggga ctgaaagcgc ccattccgcc
    63241 ggtattggga cctttatcgt tatcaaatac tcttttatga tcctgacacg acggcatggt
    63301 ctttatggtt ttaccgtcgg taaatgccaa tacggtaact tccggtccct gtaaaaactc
    63361 ttcgattata acggtgtggc cggatgagcc gaacatatcg tttatcatca tttggttaat
    63421 ggtttcctct gcctgccgat aattttcaac aattatcaca cctttaccca gcgccagtcc
    63481 cgacgctttt ataaccagag ggtattgctt gtttttaaca tattgcaaag ctttttcggg
    63541 gttatcgaaa acttcaaaag cacccgtagg tataccgtat ttcttcataa ggtttttggc
    63601 aaaagcttta ctgctttcta tttcagccgc tttagctacc ggtccgaacg cacgtatatt
    63661 tctttcgttc agtttatcca caagccccaa agctaacggg tcatcgggag tgaccacaac
    63721 caggtccaca gggttgtctt taagaaaatc cagtataccg tctatatcgg tagcctttat
    63781 gttgacgcat tgcgctattt ttcccattcc ggcattaccc ggagcacaat atatcttttt
    63841 aattttcgga ctctcggaaa gtttttttac tacggcatgt tctctgccgc ctccccctat
    63901 taccagtacc ttcatctttt acctttctca gtgtaaaaag tgccttattc ccgtgaatat
    63961 catacagata ccaaattggt ccgctttggc aattacctcc ttatcggcta acgatcctcc
    64021 gggttgtatt atgcattcca cgcccgcttc tttggccgct atgacacagt catcaaacgg
    64081 gaaaaacgca tccgatgcca taacgctgcc tttagccctt tctcccgcca aggatatggc
    64141 tatcttggcc gctcctatcc tgctcatctg tccgggacct attcctattg aagctaagtc
    64201 ttttacaaga actatcgcgt tggatttgac atgcttgcat acagtcattc cgaaaacggc
    64261 atctttcaac tgctgttcgg taggctgttt tacggtcacg actttaatat cctccgggtt
    64321 tacaacgcaa ttatctttgc tttgtacaag cattcccccg cctactttct tcataatata
    64381 ttcgttttca ttgttatatg ttatgttatc caatctaaga attcttaaat tcttcttttt
    64441 ccttaataat gtaagagctt catctttgta atcgggtgca ataactattt ccaaaaatat
    64501 ctcattgagc ttttcggcag ttactacatc caccgttctg ttgaatgcga ctattcctcc
    64561 gaatatggaa accggatcgg aatcatacgc ttttttgtat gcgtcaaata catttttacc
    64621 cactcccaca ccgcaggggt tagcatgctt taccgccaca catgcaggtc ggtcgggaaa
    64681 ctctttgagc atggacaatg cgccgtcggc atcatttatg ttgttatatg aaagttcttt
    64741 gccgtgcagt tgcaccgcat cgcagatggt tccctttgtt attgcgggtt ctttatagaa
    64801 agcggctttc tggtgaggat tctctccgta tctcatttcc tgaagcttct cataagtaac
    64861 ggtgaaagta ttctttaagc tgtaatctcc tgtaagcttg ttgaaatggt cggctatcat
    64921 tgcatcgtaa ttagcggtgt gttcgaatac ctttattgca agtctaaaat tggtctcata
    64981 agatactttg ccgttttgtt ccaattcatt taatacactt tcataatcag aaggatcggt
    65041 gataaccgtg actgttgcat gattcttggc accgcttctc aacattgccg gtccgcctat
    65101 atcaatattc tcaatagcct cttctttggt aacattgctt ttctgtacgg tctttttgaa
    65161 aggatagaga ttaataacca acatatctat aggggtgata tccagactct ccaatgtatc
    65221 catatggtct tttttatctc ttatagctaa cagacccgca tgtatattgg ggtgcagagt
    65281 ctttactctt ccgtccagac attctttgaa tccggttatg ctttcaatgg gaataacctt
    65341 tattccgttt tcgctaagta cttttgaagt gccgccggtg gaaattattt ccacacccat
    65401 atcactcaat ctctttgcaa attccgcaat tccgcttttg tcgcttacac tgatcaatgc
    65461 tcttctaatc atgttatctt tcctttatat atatattttc ctcaatatct tttaactttt
    65521 tgacatcccc ggctaaagcc acatatccta tctcgtatat ttcgttctta aactgttttt
    65581 gtattttctt cgcatcttct ttgcttaccg ctattaccgt tcctataccc atattgaaag
    65641 tacggtacat atcctcttcg gttatattac cttgcttcat tatgaggtca aatacgggta
    65701 acaccgccca gctgcccttg ttgattacgg gagttaaatt gttgtcttta aacatcctcg
    65761 gtatgttttc cacaaatcct cctccggtaa tatggctcat tccctttatt ttagcaacgg
    65821 tccttatcct tttaatatct tttacatata tctttgtagg tgtaagaagc acctcaccta
    65881 tggttgcttc atatccggga tatactttgc ttaagctttt tgcatcctct ccgaatatct
    65941 tacgtactaa ggaaaaaccg ttgctgtgca ctcccgttga tgccaatccc aaaagcacat
    66001 cgccttcttc tatcaacgat ccgtcaatta tatcaggctt gttgaccgct ccgacacaaa
    66061 atcccgctat atcgtattcg tcttgtttat agaagccggg catctcggcc gtttctcctc
    66121 ctaccagagc acattctgcc tgtatacatc cttcggcaac accttttact atcttttcaa
    66181 ttatatcggg ataattcttg ccgcatgcaa tatagtctaa aaagaataac gggtcggctc
    66241 cctgacacag tacatcgttg acgcacattg ccaccgcatc tatccctatc gtgtcatgaa
    66301 ctttcatgga atgagcaagt ttcagtttgg tacccactcc gtcggttccc gaaaccaata
    66361 ccggggtagg atatttattc aagtctattt ggaataatcc ggaaaaactt cctattccgc
    66421 ccagcacccc ttcggttacc gttctttgaa tatgcttttt aattaaagcc acagactcgt
    66481 aacctgcttg aacatccact cccgcttctt tgtatgcatc tttcacagtg attatcctcc
    66541 taaaactctt taggtatatc cacgggatag actcctgtaa aacaggaagt acaaaaatcc
    66601 actttggaat tgacaggtgc tttgataatt ccttcataac tgatgtactc taaactgtcg
    66661 gcacctatcc attcgcatat tgtctcttta tcattcttgc tggccagcaa ctgtcctctg
    66721 ctggaagtgt ccactccgaa atagcaaggg tatttatacg gcggagagcc tatccttaag
    66781 tgaacttcct tagctcccgc ttccctcagc attctgacaa gtgtcttggt tgtggttcct
    66841 ctgactatgg aatcatcaat gagtaccact cttttacctt tgacctcggt cttgaccggg
    66901 ttgaatttta tctttaccgc tatttctctg gcattctgat tgggggctat aaacgccctg
    66961 cccacatatt tattccttaa cagacctgca ggtgcaacta tattactttc ctgtgcaaac
    67021 ccgtatgcca tatcggttcc gccgtcggga atgcctatta ccacatccgc ctgtaccgga
    67081 tgttcttttg ctaaaatcct tccggcattt acccttgcgt catgtgtcga tgccccgtca
    67141 acaatgctgt ccggccgagc aaaatataca tgctcgaata tacataaact gctctttttg
    67201 cagtcaaaca accttcttga ctgaataccg tcggaatcta tcagtaccag ttctcccggc
    67261 tccacatctc ttataaattc tccgtcaacc gcatctatag cggccgattc gctggaaata
    67321 acatatgttg caccgttaat cctgcctata caaagaggca ttatacccag aggatcccta
    67381 agacctataa ggaatctggg ggtaatcatg acaatggagt aggagccctg aatatatttc
    67441 accatctgct ccaccgaatc ctctatatgt tcgctgctta tgcggaactt ggtcagaagt
    67501 gcagccagta cttcggtatc attggtggta tggaacatac atccgtccgc ctccagtttc
    67561 cctctcagct tattggcatt aattatattg ccgttctttg ctatggccat atatccgctg
    67621 atatacttca gtaccaacgg ctcgatgttt cctttggata catccccgga cgaggaatac
    67681 cgcacatgac ctattccggc atcacccggc agtttgtcca gatcctggct cttgaaaacc
    67741 tcgggaacaa gacctttatc tttatgccac tctattaaat ctttatctct gaccgctata
    67801 ccggcactct gctgtcctct gtgttgaaga gcatacagtc cgtaatatat ttgctccgct
    67861 atgttaaccg atttatcttt tgaatatact ccgaagactc cgctcatatt acttgttgtt
    67921 tatctccctg tccgcttgca ttaccttttg ttccatttcc tttttgaact gttgcaattt
    67981 attgcttaat tcttcattat tcagagccag tatctcggct gccaatattg cggcattttt
    68041 tccgccgtca atcgccatcg tggcaaccgg tactcccgca ggcatctgca ccgttgaaag
    68101 gagtgaatcc atgcctccta aatcgcttgt cttaacggga atccctataa ccggcaacac
    68161 ggtatgcgcg gctatgactc ccgctaaatg ggccgctttt cccgcagccg ctattatgac
    68221 cttaaaacct tcacttttgg cgtttacggc cagttgcttg gccatatcgg gtgtgcggtg
    68281 cgccgaacat acccttacac tatattcaat attatattcc tctaaaaatt tcagaccggg
    68341 ttctaccgtt ttactgtcgc tgtcgcttcc catcaatacc aatactttca tttaatcctc
    68401 ccattaaaaa agcagtctcg tagtgactgc accataaaaa agaacgcaat ggaataagcc
    68461 actcatagtc gggtctgagg tgtcccggta gaaacgttgc agaccgtttt ttgcaacata
    68521 tacgagttct cttcaagaaa atattttatc actccctccc tttatgtgtc aacacgaacg
    68581 ggctgttttg tcaataacct atgataattt caccccatga actaaccagt gaatatttca
    68641 ccccccctgt actgtaaaag tgaaatattc ttgaaaagtt tatatgattg gaggtatgaa
    68701 atatcatatg tcaacaaagg aattaaaaag gttatttgtt ttagagcaga gtgttgaagg
    68761 caaattgact gtaaaagaag cagcagaatt acttcattta actgaaagaa ggataaagca
    68821 gttaaagaag gagttcagag agaaaggagt tacagctgct attcatggaa atgctaacaa
    68881 gccttctcca aaacgcacag acaaaaccac tgaagagaga attttaggaa ttaggaaaaa
    68941 ggaaccatac tgttcaagca acttcaatca ctttagagaa atattgaaag aacactttga
    69001 cattaggatt tcctattccg ctctttatcg tttacttacc gaacatggga ttaaaagccc
    69061 taagaaaaga aggaagaagc gtagggtcta caaaacaaga gaaagaagac ctgcttttgg
    69121 catgatgtta caagcagatg catcaccttt taattggttt ggaggagaga aaaagttttc
    69181 attacatgga ttgattgacg atgccacagg taaactcaca gggctttatt tatgcgaaaa
    69241 tgagtgcctg cttggctacc ttgaagtact ccggcaaaca ctcatttcgt tcggaatccc
    69301 acaatcccta taccctgatc gtcacagtgt cttcttttat acaccacaag acaaagacaa
    69361 actctctatt gaagagcaac tttcaggatg tacaaagaag gtaacacaat ttgggaagat
    69421 tgtcgagcaa ctcggcattg atatgtttcc tgcacagtcc ccacaagcta aaggacgtat
    69481 tgaaagaagt tggaatactt tacagagccg tttgcctgta gaacttaaaa tgaggggtat
    69541 tacgagcatg gagaaagcta atcttttctt tgaaaaagaa tacattaatt tgttcaatga
    69601 gcaattttct gttgagccaa aggagagcta ttcagctttt gttcccattc cgcacactat
    69661 tgatttagac cgtttattaa gcgtcaaatt agaacgttct ctttcaagtg ggtctacaat
    69721 atctatctgc aacaaactct ttaaaatcga acaggatcgc ttttctgcca gagaaaaggt
    69781 tacagtcttg ctttccaaga aacacggatt aagagctctt attagcggtg agttctatcc
    69841 tatttatccg attgatgata ttagttatgc agaagatgtt gtcagaaccg gagactttcc
    69901 tactgtagtt gtaagtctgt tgcatcactt ctttcttagt gatgtcagat taggtcattt
    69961 tccggaaggg gtgaaatatt cacagaataa ttaaacactt tagggggtga aattatcata
    70021 ggttattgac atacgaacgg gctgtttccg tcatatacat tatttgtttc aacctgtaaa
    70081 aagcaggagt tttattaaaa aatgtccgat attaacaata cctatcaata gttttttctc
    70141 attttaaaga aaaatccaaa ttttacatac ttttttctca ttgttagaaa aaactccgaa
    70201 ttcttccgag tctttctctt ttcagaaaat ttctcacttt acataactta atatttcaaa
    70261 tttacatttt acattgtttt aactgttcaa tgtcctcact ctttacatcg acaatgtatt
    70321 ctttaggtat gaaaaagaaa acaaattatt gtaaaattaa atgggaggaa ctaagaatga
    70381 aaaaaacaat aacaatagtt ataagcctgg ttctgttact tgccatgctt ggcggatgta
    70441 ctcagaaagg gtttgataac aaaacggcta taaccgttat agctcgtgag gaaggttcgg
    70501 gcaccagagg cgctttcatt gaactgttca agattgagaa gaaagatgcc aacggcaaaa
    70561 aagtagatca cacgatttct actgccgaca tcaactccag taccggtgta gtattgacta
    70621 ccgtagccca gaacaagtat gccataggtt atgtatcttt aggctcactg aatgatacgg
    70681 ttaaggcttt gcagattgac ggggtagctg ccacagttgc aaatatcaaa aacggaacat
    70741 acaaagtttc ccgtcctttc aatattgcaa ccaaagacgg attatccgat gttgctaaag
    70801 acttcataga ctatatattg tcaaaagaag gacaggcagt tgtagaaaaa tccggatata
    70861 tttcagcagt ttcgggcgaa gcttatgcag gcaagaaacc ttcgggtaaa gtaaccgtag
    70921 caggatcctc ttcggtaacg ccggttatgg aaaaacttaa agaagcatat attaaactta
    70981 atcccaatgc cgttatagag attaaccagt cggattccac tacaggtgtt aacagtgctc
    71041 tcgacggaat atgtgatatc ggaatggctt caagagctct taaagacagt gaaattgaaa
    71101 aaggagtgaa acctacagta atagctattg acggaatagc ggttatagtt aataatgata
    71161 atcctacagt aggcatgaaa gctgaagaag taaccaagat ttacatcggc gaaataacca
    71221 agtggtcgga aataggaaac tgagattttg agtagtaaaa aaagtaaaag tgtaaaagaa
    71281 aacatcatga aggcgatctt ttttataagc gccttcatgt ctgtcgttgc ggtaatactg
    71341 atatgtatat ttctctttgc caacgggctt ccggcaatga aaaaaatagg gtttgctgaa
    71401 tttcttttgg gtagaacatg gagacccgga aatgacatat acggagtatt tccgatgatt
    71461 attggaagcc tctatgtaac tgccggagcg ctggtgattg gcgttccggt cggaattcta
    71521 tcagctatat tcttggcgcg gttctgtccg cccaagttat ataaagttgt taagccgatt
    71581 ataaacctgc tggcaggtat accctccgtg gtatacggct ttttcgggct tatggtcatt
    71641 gtgccgttta ttaggaatac tttcaatgtc gcagggacca gcatgttggc tgcttccctc
    71701 gtccttggta tcatgatact tcctaccata atcggcatgt cggaagcgtc cataagagca
    71761 gtacccgaca gctattacga aggttcattg gccttaggtg caagccatga gcggagtgtt
    71821 ttttattcct tgctcccggc tgccaaatcg ggaatcctgt ccagtgtaat attgggcatg
    71881 ggccgagccg taggcgaaac aatggcagtc gtaatggttg caggcaacca gccggtaatg
    71941 ccgtcaggtg tgttaaaagg cttaagaaca cttactgcaa acatagtgtt ggagatggga
    72001 tatgcagccg atctgcacag agaagcactt atctccacag cggtggttct ctttgtgttt
    72061 atcttgatta ttaacttact tttttctatt ataaagaaag gagttaagtc atgaaagctg
    72121 caaagagaca atcggttttt ttaagatgct tagtatatat ctcggccgga cttaccataa
    72181 cgacggttgt aggtatttta atatatattc tcattaaagg cgtgtcgcat ttgtcgtggg
    72241 atttgttcgc atttaagtac acatcggaaa atgtatcgct ttttcctgct cttgtaaata
    72301 ccgtcacaat gactgttgtc tcactgctta tagcggcacc gatggggata ttctccggta
    72361 tatatcttgt tgagtatgcc aatcgtgaca gtaaggcggt aaaacttatc cgattaacta
    72421 ccgaaacatt atcgggaatt ccctctatag tattcgggtt attcggttat ctgctctttg
    72481 tctcttccgc ttcgggtttc ggctggggat attccatgct ggcgggagct ttttctctgg
    72541 ctataatgat actgccggta ataatgcgtt ccaccgaaga agcattaaag agcgttccgg
    72601 acacttacag agaaggcagt ttcggattgg gagcaggcaa acttcgtaca gtattcaaaa
    72661 ttgtgttacc cgccgcaatg cccggtatac ttgccggaat aatattgtca ataggaagaa
    72721 tagtcggtga aaccgccgta cttatataca catccggaac ggtagcacat atacccaaga
    72781 acctgatggg ttcgggaaga actctgtcgg tgcatctgta tgctctttgg tcggagggaa
    72841 tgaataccga gtcggcatat gctacagcgg taatattggt agttaccgtc tcgttgctaa
    72901 atgcattgtc cgcttatctg gcaaaaaagt ttacgaaagg aaatttgaat ggataaattc
    72961 gtaatagaaa atcttgatct tttttatgat acatttcagg cactgaaaaa agtgaacatg
    73021 tctataccga tgaatgaaat aaccgctttt atagggcctt cgggatgcgg taagaccaca
    73081 ttgcttaaaa cgctcaacag aatgaatgac cttatagccg gttgcaaaat acaaggcaag
    73141 gtattgttgg acggggtgga catatacgga gacattgatg tcaatcttct cagaaagaga
    73201 gtaggtatgg tgttccagaa acccaatcct ttccctatga gcgtatatga taatatagca
    73261 tacggaccga gaacacacgg aattaaagct aaaattaaac tggatgagat agtggagaaa
    73321 tccctgaccg atgcggctat ttttgatgaa gtaaaagatc gtctgaaaag aaatgcattg
    73381 gaactctccg gcgggcagca acagagactg tgtattgcac gcgcattggc agtggagccg
    73441 gatgttctgc tgatggacga acccacaagt gcattggacc ctatatccac agctaaaata
    73501 gaggaccttg tacttgaatt aaaaaacaga tatactatta ttgtagtcac acacaacatg
    73561 caacaggcaa ccagaatatc tgacaagacg gcatttttcc tcctcggtga ggtaatagaa
    73621 atgaacgaca cggaaatttt attctcaaac ccggctgatg tgcgtactga aaactacata
    73681 acagggaggt tcggatgatg cgcagtaaat tcgatatgca gttggagata ttgaataaca
    73741 gccttataga gatgggcgca atgtgtgaaa aagccatttc atcggctatt aaggctttac
    73801 tggagaataa tatttctctt gcaaaaaaag taatggaatc ggttaaggat ataaatcagg
    73861 cggagaagga tatagaagca atgtgtctga agttgcttct ccagcaacag cccgtagcaa
    73921 gggatttacg tcagatatcc tcaatactta aaatgataac ggacatggac agaatcggtg
    73981 tacaagccgg ggacattgca gaaatagtta cttttgccaa tctgtcgaaa aataaggata
    74041 gagtacatat agccgatatg gctgaggcaa ctataaaaat ggtaacggaa agcataaatg
    74101 catttgttaa aaaagacctt gagcttgcca ataaggtaat agcatacgat gatgtggttg
    74161 atgatctttt tgttaaagtc agaaacggca ttgtcgacct tatatcaaaa gacaagagac
    74221 atggagaaca ggcaatcgat ataataatga tagctaaata tttggaaaga ataggagacc
    74281 acgcaacgaa tattgccggt tgggtcgctt tctcaattac cggcattcat gtggaactgt
    74341 aaagatgatt tactttgtag aagatgacag cagtatacgg gaacttgtag tatataccct
    74401 taacaacacg ggttttgaag cagagggcat ggataatgca aaaacattgt atagtaaaat
    74461 atccgtaaat aaaccggagc tgatactgtt ggacattatg ttgcccgggg agagcggact
    74521 ggaaatatta aagaagttac gttcttccaa agaaacggcg aatattccgg ttataatggt
    74581 gacggcaaaa ggtaccgagt atgacaaaat aatcggattg gacagcggtg ccgatgatta
    74641 catttccaaa cccttcggta taatggagct tgtatcaaga gtaaaagccc tcttgaggag
    74701 agtatccaga aaagaagata ccgacagtgt aaccgatact tatacgtatg agggagtatg
    74761 cataaacagc aagaaccata cagttacatt ggattcggaa cctgtggttc ttactcttaa
    74821 ggagttcaga cttttatatg agcttatggc taatcagggt atagtcctga cacgtgatac
    74881 actgcttgag agagtatggg gttatgatta tcccggcgaa acaagaacaa ttgatgtaca
    74941 tatacggaca ctaagaaaaa aattaggaaa gtacggggat attataaaga caatacgagg
    75001 tgtaggatat attatcggag gcaatgcatg agaaaaaggg tttttataag catctttctc
    75061 acctcgctgc tgacaatgat acttaccttt atccttatct cctggataat gtacaacaac
    75121 actttcgaca gcgtaaaacg tgaggttcaa aacgaatctt tttatgtttc ggatgctttg
    75181 aaaacatata ataatgatac ggccgacatg ttttcatacc ttaacagagc gggtcttaaa
    75241 agtcgcaaca gaataacata tataaaaagc gacggaaccg tcctttatga caatttagcc
    75301 ttagctgcgg atatggaaaa ccactcggac agaccggagg taatagaggc tttacaaacc
    75361 gggcacggtg agattacccg atattccgat actttgggag aaagtatata ctattatgct
    75421 ataaagttgg aagacggcaa cattttacgc attgccgcaa caaccaaaag catcttggga
    75481 atgatgacag gttccgcgat atggattatc ctcatattta taactttcat atctctatcc
    75541 gtaatcatag caagattgtt gacaaaatcc attgtcagac ctattaataa tctggatttg
    75601 gataatcctt tgtcaaataa gacttatgat gagttatctc ctttgctctt gcgattggat
    75661 aagcaaaaaa ccgaattgat ggaaaatgtg caggaaatat ccaaaacccg catcaatttt
    75721 gcttttatat ccaacaatat gtctgaaggt attgtggtgt tttccgaaaa aggctatata
    75781 acgggtgcaa atagaagtgc cagagacatt ctcggcatag atatacaaca attcttcatg
    75841 gaaggttgca gagatctcgg ttatatcaaa gcagtccgct ctgcactcga gggaaaacca
    75901 atgactgcaa aatttgaaaa aagcggtttt gtatatcagc tttcggcaag tccggttaaa
    75961 gagaatcccg aaaaacatgc cgctcttctt ttcatcaccg atataacgga gaaagatcag
    76021 gccgaacaga gaagaaaaga attttccgcc aatgtctcac atgaattgaa gacacctctc
    76081 acttccatat ccggttatgc cgaactcata tcaaaaaaga ttgcaaagaa agaggatgta
    76141 ccgcgttttg cggagatgat acacactgag gctacaagac tgcttcaggt tattgaagat
    76201 attatgaagt tgtcccgact ggatgaatcg gatctggaga aggaatttga ggatgttaac
    76261 ctgagcaagc tctgcacctc ggttattaat gatttacagc agatagcgca gaataaagat
    76321 gtagtgatta aatccgattt gaaaaatgtg tatataaacg gctattctcc gatactttat
    76381 gaaatgctct acaatctgat tgataacgct ataatttaca acaatccgaa agggtacgtt
    76441 gatatttcct tagcagaaga ggaagatgaa atcattctta ccgttaaaga taacggaata
    76501 ggaataccgc cggaacatca gcccagaata tttgaacgtt tctatagagt ggacaaaagc
    76561 cactcaagag atacgggcgg aacgggatta ggtttatcca tcgtaaaaca tgccgcagca
    76621 ttgcataagg cggatattga tttaacaagt actccgggcg aaggtacaac ggtaaccgtc
    76681 actttttaca acggtgataa ataatataaa cggcttttta ctttaattct tttgctaatt
    76741 tctcaagacc tgcttcaaag tttacaccga accgtatgtc ggtttttgct ttatatgaac
    76801 ggataagact ttctaccgtg aaatccactg ctaccgttgc ggaatctatc aggtttttac
    76861 ctttaatcat tgcggccaac aatgcgctgc cgaagacatc gcccgttccg tgataatatc
    76921 cttctatacg ttctctttta tagaatccga attgatcaac ggtcttatca tatgtagccg
    76981 cacccagttc cttttcattg taataaacac ctgtcagaac cactttatcg ggacccatgt
    77041 ctgcaagacc caataacaaa tcccttatat accgctcatc atacggtcct tccttgtatt
    77101 gcttatccag cataaaacag gcttctgtaa tattgggaac aaccgcatcg gctttggaaa
    77161 caagtcttgc cattcctttg ggaaaatccg gactgaacga tgcatagagc ttaccgttat
    77221 cggccattac cggatctact aatattaagg tatctttgtt cttgtatata tcaaatatat
    77281 ccgatactat gtcaatctgt tcaaaagatc cgagatatcc cgtatacagc gcatcatacc
    77341 aaatatccag agttttccag tgctccacta taggcattat atcctctgta agatccctgt
    77401 atgtaaaacc ggtaaatcct ccggtatgtg tagacagcac agccgtcggg ataaccgtgc
    77461 actctatccc ggctgccgat attatgggca gagccaccgt caacgaacac cttccgaaac
    77521 aggatatgtc atgtattgcc aatactcttt tttgtctttc catgttgcac ctcattcttt
    77581 cgtacaaata ccaaatcctt gctactttct aatatataca atagccatat ccgtgtcaaa
    77641 tggttttgac tgcattgtat gagagattaa atatcggatt aaataaaaac cgcataatac
    77701 ttttatccgt tatcttttag gcagtatctc tatccagtat ccgtccgggt cgtttataaa
    77761 atataaaccc attgctttat tctcgtagca tatgcagccc atatccttgt gttttgcata
    77821 tgaagcatca aagtcatcgc ttatcattgc cagatgaaac tcattatctc cgaggtcgta
    77881 tgacggcttg tcaaaatcct tcagataggt aagttccagc atgaaacttg tttcacgctc
    77941 ctttaagaac actaaagtga aggaatccgt ctcttttctt cggacctctt ccaatcctaa
    78001 agcttcttta tagaagacta cggatttgtc caaatcggtt acattgaaat taaagtgtgc
    78061 aaatgcgtgt ttcatgcctc ctcctcaaag aaaaatacct gattaatatg ctctattctt
    78121 actattttct cataatggtc actcagtgac ttgttttgga atattttatc ggttgtggag
    78181 taattctcga aataatgcat gggaaaaact ttcaatgttc tcgtgttatc cagaaagtat
    78241 ttcaaccctt tatcatagtg ctcttcctga cgtaaatcca caggatagaa agcgtaatca
    78301 atcaccctgt ttttcagttc cgctatcctt ttacggtaag ctttactcat tctttcattg
    78361 tgtctgtcac tctcttcttt ccatatccaa aggtgcaaat ccccggcatg gaagaatacc
    78421 ttattatcat gggttacaac atatgccact ccggcgtcgg ttgaggccaa tgtctctata
    78481 tgcacatccc ctatatccgt ttccctgtta ggcatcagat atgttattct gtcctgaaca
    78541 ttcggaaatt cggactttat tctgtctccg aaatttctcc ttatatcata tgccatgata
    78601 aagaatgccc tcgggttgtc ggcgaaaagt tccaatactt tcatatcaaa atgatcctca
    78661 tggctgtggc tggagaatat gtaaatgtcc ttatcaaaat gctcgggaag ttgccctttc
    78721 ccgtaatagt caaaaagtat aaacgctttc tttgtttcta taaggaatgt gctgtgtatc
    78781 agatgagtta tgtacatttc ttcctcctcc ccttaccgta ataatactgt attattacga
    78841 ttttaacgca ggtcctatta gttaatggta tcacggcggg aatacttatt caattaaaat
    78901 gcggaaaaac acttgttaat aaattaacca tcttttgctt tttatttgac ataacataat
    78961 atatgttgtt caataggaaa aacaataaag gagaaacaca tgaccgataa gaagcaaaac
    79021 aagttttgga gtaaagtaaa gaaatactta aaccatgtct ttatagacgg actttccgga
    79081 atggctttgg gattattcgc cactttgata atcggtacca taatagccca gattggatca
    79141 ttgataggcg gtaccgccgg taaatatata aatatggtgg cttccgtagc taaagctctg
    79201 accggtgccg ggataggatt cggtgtggca tataagttca aggagtccat attggtatcg
    79261 gtatccgcag gcgttgcggg aatgatagga gcatttgccg caagcatatt atccggtacg
    79321 gcatttccgg gagcagggtt gctgcttaca ggtcccggtg aaccgctggg tgcgtttttg
    79381 ggtgcattag ccggaataga agtaggtcat ctggtatcgg gtaaaacaaa ggttgatatc
    79441 ctggttactc ctttggttac aattaccgta ggggcggcgg taggtctgct cataggtccc
    79501 cccatttcca aattcatgga agctgtcggc gccgtaatca actggggagc ggaacagcaa
    79561 cctttcatag tcggtcttgt cgtttcagtg gtcatgggat gtgttttaac gttgccgatc
    79621 agttcggcag ctttgggaat aatattaaag ctttccggtc ttgcagcggg agcggctgcc
    79681 gcaggttgct gtgctcagat gataggcttt gcggtaagca gttacagaga aaataaagtg
    79741 aacggattaa tagcgcaggg actgggtact tccatgcttc agatgcctaa tatagtcaga
    79801 aaacccgtaa tatggattcc tccgatagtg gcaagtgcgg taacgggacc gatatccgcc
    79861 tgggctttga gaatgacatg cgatgcaacc ggttcgggaa tgggtacatc gggactggta
    79921 ggccccataa tgactttcaa aaccatgctt gcagacggat ttcccgtctg gtatacaata
    79981 ctttctatta tcggtgtaca gttcatactg cctgccgtga tttcgctttt tgtatcggaa
    80041 ctgatgagaa aaaaaggtat catcaaactt ggtgacatga aattagatat gtgaaatacg
    80101 gttttattct cttcaggcat ataattaaac aaattgaaag aaaaaaataa taaaaggaaa
    80161 caatttacag aaagacaata ccgcccgtaa aggcggtatt ttttagaatg tgtggttttg
    80221 tatatctttc aaaaggggat tctatatatc cagtaatttt aaaatagctt gtttcgactg
    80281 tacgccgact gctcttctta caacatttcc gtcctttata acaaccagag tgggaatgct
    80341 cattatgccg taacgttgtg cgagctcggg ttcttcgtcc acattgacct tacctataac
    80401 cttatcggtc acttccgctg ctaccgcttc aattgtcgga gcaatcattc tgcacggtcc
    80461 gcaccagcct gcccaaaaat ctataagtac cgtcttacct gattttaaaa cttctttttc
    80521 aaaattgtct ttcgttatct tcaatgttgc cattatgtgc ctccttttaa gtttgtcacc
    80581 aaactttcat ttacccatat tatatacccc tctataggca tcaacagtga ctttatcaca
    80641 ttttttagta aatattgatt gtataattag gacatagtat aattaaggag caaggaggac
    80701 ttaaaaatat ggaaaataac aatgtaagaa gaatgcccct tttgggagat gatgcaccgg
    80761 cttttacagc ggtaactaca cagggtacaa ttaactttcc ggcagattat aaaggaaaat
    80821 gggtaatact cttttcacat ccggctgact ttacccccgt atgcacaacg gagttcatga
    80881 ctttcggttc tatgatagat gagtttaaag ctcttaatac cgaacttatc ggtctttcgg
    80941 tagattcact atatgctcat attgcatggc ttcgcaaaat acaggaactg gaatggaacg
    81001 gaaagaagaa tattgagatt aaattccctc ttatcgaaga tatccgtatg gaagtttcca
    81061 atctttacgg tatggttcaa cccggacagt ccaacacaca ggcagtccgt gcagtattta
    81121 taatagatcc taagggaaag attcgtacaa tattgtacta tcccatatct acgggaagga
    81181 atttcgatga actcaagcgt attatccagg ctttacaaac agccgatgcc gagagttgtg
    81241 caactcctgc ggactggaga ccgggcgatg atgttatagt tcccacagca ggttcctgcg
    81301 gagtggcaaa agagagaatg gaatcaaaaa gtaaggatat gtactgcctt gactggttcc
    81361 tatgtttcag aaagctgaag aaataaggtg aaaacgtgca tgttcattat gcatgtagaa
    81421 taacaataca acagttgaag aaagcaggtg aaaacctgct tttttctaca cttcttctat
    81481 gtttatccct aatatccttt ttcattaact ttacgattct ttatatatct gtacagaaaa
    81541 atcaccagag atataaatat cagtattaaa taaagagcgg gataaaagta atgcttaacc
    81601 caagatttat aaggcaatct tctgaaagga agaatgaaca gtctcattat gcagaaaaga
    81661 tttgcatata ctaaaaaagc actcccttca ggaaacttgt cgctcttttt ttgcacaatc
    81721 aaagcaggta tcaggataat tatagcaaat gccatctcaa acaactgagt gggaagtacg
    81781 ggataacttg ccagaccgcc tgcatcggcg aacttgggat atacaacacc gaatatgctg
    81841 tttgtgaatt tgcccgaaca acatccgttc aaaaagcaac ctatcctggc tatagcaaaa
    81901 gaaacggcgg cgggaacaat gcataaatcc aaaatgccga atataggtat acgaaacaga
    81961 agacaccaca gtactaccgc aagcagagct ccaaaaaaac ctccgtatat tgatagccct
    82021 acaaactccg gctcaaaaac cgaaatatcc tcataagctg cgggattaac aaagatgttg
    82081 aacaatcggg ctccgataaa gaagaataca agcaggaaaa cagccaaaat tgccgttttc
    82141 catactttta tcttcttctt gccgagtaga agaaacagaa tggttgttcc cgatataacc
    82201 gccagaattg caaataagcg gtaggatgca acggtttttc cgaatatgac taaagaaggt
    82261 ttcatatcat tttatttctt aacaaacgtt gctcgtaatc tgacatgata atcggtcatt
    82321 ttcattcccg gaatcgtcat cattatatta cccgttacgg atatgccttc cctattcggc
    82381 agatatgcag ctataaatac acctctgata taaagatcgg taagtacaat tctgccttta
    82441 tcttcataat aactgaagtt catagagtct aatcctactc tgtcaaaata tatgcttcct
    82501 tcattttcct cctcatgggc ggagattgtc attgttgatt gcagagtttc tcctatcagc
    82561 tcttctccga cagttattgt aatctccgca gcggaatcac atcctatact ttttgccatt
    82621 tcatccgcct tttcctgcaa tctcgctaat gctttttcaa aattttctcc gataaaaaca
    82681 tcctcgataa ataaggttcc gtccggataa ctgcccaaaa gttcctcaaa tgtaggatgt
    82741 aaatcgggta cggttattac agtatcgggc attctgaatg taaagtttac ggttctgtcc
    82801 ggaaggttct ttcttaacga tgaagtcttt aagaatatgt tcaatgtatc gggcatgccg
    82861 gaaagaatgt atcctaaata agtcgcttca agtttaggat tttctccttt cttaatcatg
    82921 gggaaacgcc actgattgga aaatcttgca atttgcgttt ttgaaagctt tgtaaccaat
    82981 ccgtcgggta cggcaaatct cattgttgca cctttatact tatcatagaa tgtatcaggt
    83041 tgatcggata caatctcaaa agtaatggtc ttgtttaact tttctttttc cttcaaaagt
    83101 gtctcttcga actcagcctg cattttagca aatatcttct tcttgtactg agctactatc
    83161 ggtatcatac gcttccgcaa gttttctctt acggtggctg taacaatccg ttcatcttcc
    83221 tcactcaaag aaggatactc cacacgtctc atcgggtcgt tatccgccct ccattgattt
    83281 cctctgtaag cggcgggtgt tgccgcccat aagcgcggat agaaattaat tgcattggct
    83341 acagctgtaa taaatgcttc cggatcatcc ttgttaagct gatacaactt gtcaataagc
    83401 cacaaccact cggcatcact gcccgcttta tatgcgtaat agttcattcc cggcgaatta
    83461 tacatgtaat accatgcccc gcttttgctt tgactatatc tatccccttt cttaatgcaa
    83521 tacagataag actcgtttat aaaatcttcc tgtgttttta ccgctaaatc aattttcttt
    83581 gagcgtttgt gagaaatggc tgctccggca atattgactg ccgcccatcc tgtaaaagcg
    83641 acagtagcag ccacagggcc tccgatagca ccggccattg caaaccaact catttccatc
    83701 agccccgtta acacggtttg tgccgctttg cttccgctgt ctaaaataaa atactcataa
    83761 cttgctttta ataaaaccag tccgaaccct attttagaca gagtcgaagt cagtccctca
    83821 atctgtgtca atggaatact gttcataatg gcctgcatgg tcaccttgcc ttcataagcc
    83881 tgactggtga aatttaaaaa ttccaatcct actgttacca tgtcggaatc ggtaggtaaa
    83941 tttccctttt ttaatttttc atagaatttt aaagggtcac cgttctggaa aaagctcctt
    84001 ataacagtat agttaagtgc tgccgttgca aatcgcgaat cggatttgga tattttgaaa
    84061 agagcaaaat ccgtaaagtg atcggtgtaa ataacaacct tattctcttc tttgaatgtt
    84121 tcatgaggta gaatcctcca cttggaagat tgatcatcat aatatgcagc acagacatcc
    84181 aagtcatcat aatcggtcat atccagcggg atggctatgc ttactatacc tgcaaactcc
    84241 gaagattctt catcatcggc agtcaatctg tatgatgcca aggcattgcc catttcctca
    84301 tcggatgcac cttttgtcgg acttatcttc agttcaacat cttcatccga tgtcatacct
    84361 tcgaaatcta cagatatacc ctccgcctgc acaacgggac tctcccgtcc tacagttaca
    84421 accgtacctt tctgagctct tcccgccttg gtcttatatc tcctgttacg gtctataata
    84481 cccggaacat acattattcc aagaactata ctgactatca gtacaacggc tataacggat
    84541 atcaatactt ttttcttcag attcttaaca ggtttttttg cacgcttctt aggctgaggt
    84601 gttacttgag ttatcggagt tccgcagaca gtgcaaaatc cggttccctg ttttaatatt
    84661 gtgttacact tcgggcacaa taaatcctga ttaaattttt gaccgcaatt agtgcagaaa
    84721 gttgtatcat cattgttgtt tgccccacaa tatctacaat acataatatt gccctccctt
    84781 tgttatttta cattacaaaa gtattatacc atatggacat ccgtttcagg tcttatcggt
    84841 gtgtgattag acagccatta tggtaaaata ttaatatata cctgtaaaaa ttatgttaca
    84901 accgggaagg cggataagtg agtaaataca actacagtaa agaattgaaa aaacctataa
    84961 gcctgggaaa tattaacttt gagaatatcc ttatccgcac tctcataaga acgggctgcc
    85021 gcctgtccga tttgcagatt aaactcttga aaggcaagga agacttttcc aggagaaccg
    85081 ttcatatacc tgtcggagat aatataaaaa taccctgtac cgttattgaa ccgaaaaacc
    85141 aaaaaataaa cttaccggca attatatatc ttcacggagg tgcattcgta ttgccgttag
    85201 caagtcttat gattaaaaat gccctatact atgccgagca cttgcaatgc agagtgtttc
    85261 ttccccaata caggctggcg ccggaatatc ctttccctat tcctttgtat gactgctata
    85321 acgcattcaa tcatttaacg gaaaatgcag acacatacct tatagacaaa aacaatatac
    85381 tcatatacgg agacagtgcg ggcggctgtc tggccgcttc ggtgtgtcat atgttaagag
    85441 acaacaaaaa atatatgcct aatgcacaga tacttatcta tcccgtaacg gataattccg
    85501 ttacatcaaa atccatggag caatataaaa atgcggcctg gacaaaaacc gctacagttc
    85561 atatgtggaa cctgtactta aagaacggac atgcgggtat gctcggatat gcggctcctt
    85621 tacacaattc ggatttcggc aaccttcctc ccgcctatat cgaagcggca caaatggata
    85681 cattgagaga tgaagcgctg tcttatgcgg ataaactgaa agatacaaaa ataccggttg
    85741 agacatattt agtgaaaggt gcataccacg gatttgatat ggatcatacc tctcctttgg
    85801 taaagaaagt actcaagcac agaataaaag taatgcgcaa ttattttgct gcaagaaaaa
    85861 tctcggacgc ttttttccat tgttaatctt ttatataaac aaccaaagac ttatagccat
    85921 aacagccatg cccgctacca ttccgtatat ggacagatgg tgctctccgt attctcttgc
    85981 agatggcaac aattcgtcta cggatatgaa taccataata cccgcaacag ctgcgttgag
    86041 tattccgctt acggtgtcat tcataaaagg catcagtata aggtagccaa tcaaagctcc
    86101 tatgggttcg gacaatcccg aagcaaaaga atacagaaaa gctttcttct tgctgccggt
    86161 ggcatagtat atgggaacgg atacggctat accttcagga atattgtgta ttgctatagc
    86221 cgcaacaatg ggtatggcca ctctgggttc ctgaagcgct gcaacgaatg tagccaaccc
    86281 ttcggggaaa ttatgtattg caatagctaa agcggtaagc actcccgttc ttttaagcct
    86341 gtctttattg acttcttcat cttccgtctc caccattttc acttcatggg gattcttctc
    86401 gctgggtata agtttgtcta taagggcgat aaacagcatc cctccgaaaa aagcgataat
    86461 agtatacaga acaccgattt tttcaccatg tacctcggta agcacatccg atgcattgga
    86521 aaatatctcc accatggata cataaatcat aacaccggca gaaaatccca ggcttgtcga
    86581 taaaaactta gtatttgttt ttttagcaaa aaaagctatg agacttccta ttcccgtaga
    86641 caatcccgct ataagtgtca gcccgaatgc caaccataca ttacccatct gctacctcgc
    86701 atcgtatctc tacagtttga agtatatcat atcgtgcagc atagtttaaa gattaataat
    86761 aatggaatta aatggttatt aagaacctta ctttatgcat gaagcggcat attgataaaa
    86821 atataacaaa gcggagacaa attgcttgaa ttcttattga tataaggtta caataaggcg
    86881 cagtatggaa tttgacggac tacaaaggaa aagagattac aaggcagtac agaagacagt
    86941 tgtcattgct ataacggtta ttttgttact tatacctttt atgatatggc agaacaacag
    87001 cctgacagta accgagtatg acaaatacag tagcaactta cccgatgctt ttgacggtta
    87061 tacaattgtt cacctttcgg atttgcacag taaagatttc aaaggcaaag gtacggtaaa
    87121 gataaacgca ctcaatcccg acattattgt aataaccgga gatcttattg atttacataa
    87181 ccgaaaactt gatgtagcgg aagacgctgt taaaatgctt actaatattg ccaaggtata
    87241 ttttgttacg ggaaatcatg aaatactctc ccccatttat ccttctcttc ttgaaatatt
    87301 cgatgaatac ggagtaaccg ttctggataa cgagcaacaa aaaatatata tcggggagca
    87361 aagtataaca atctacggat atgccgattg cgaatacggt atagataccg atgcggctct
    87421 ggacaaggag gatttcaacc ttgttctctg tcacagacct tatgctccgg aagtttttaa
    87481 taaagtaaat gcggaccttg ttttaagcgg tcatgcacac ggcggacaaa tccgtcttcc
    87541 gtttataggc ggattgattg cccccgatca gggatttttc cctaagtatt atcagggaat
    87601 gcatgattat ctggacacct cggttgtgat aagcagaggt ctcggcagaa gtatattgcc
    87661 gttaagatta ttcaacagac cggaaatcgt ggttattaca cttcataagt aagtttatta
    87721 aacactcacg tgaaactgtt ttttcactta ttttttcttg ttttaagaag attttttggt
    87781 gaacttgtaa taacaacacc cagtataatc agtaccgctc ccaaaatgcc caatacggtt
    87841 attttttcct tcagcagagt tgcggccaat atcatagtca caaaaggtat ggcatagata
    87901 taactgttgg taacagtgat tccgagtttg cttaccggta tgttccatat cagataacac
    87961 aaaccgctgc ctatcacacc gaggaataaa atgctgataa cgagtttgag atcggaaaaa
    88021 gctgtaaaat caaacggctt tctttcgatt aagagaaaag gagcagtcgt tataatcccg
    88081 taaaacatta cttttcgtat cagcagtatg ttgtcatacc tgttaattac ttttttcaga
    88141 atgagagagt aagccgccca tgacagagca gataaaaaag ccaataaatc tcctatggga
    88201 ttgaatttca agataaatgt tccgttgaat acaacaaaag caactccgat aaaagcaatc
    88261 acgaaaccgg ctaccgtatt aacctttaac ttttcatcct ttgtaaatat gtgagccaga
    88321 agtgctatca gaataggaga tgatgctact ataatgctga catttgaagc ggatgtcatt
    88381 gtaagtgcct tgtattccgc aataaagtac agtgagcaac cggtaagtcc cataagaaag
    88441 aataccggtt catctttcca tataattttg gtaaatttcg gttttatcag cagaaggact
    88501 atatacgcaa gtataaatct cattatcata acctgaaacg gggtaaagta atccaacata
    88561 aatctgctgg cgataaaagt gcttccccat accataatcg ttatcagtgc tgtaaaatgt
    88621 gccccaaact tgcttttctt atccatcgct ccctttatcc gaccgataga ttatatcaga
    88681 ataatcatta aagcaataga actacattat tgaattctca cctgctctga aacattctat
    88741 actacttcaa ctctgcatat tttagaacat ccctgcataa gtatatgagc agtagggata
    88801 aaaggacatt gcatactgta attgccaaaa acggtaatat ataatccgac agcagaatcc
    88861 accccgtatt gtctatcagc aatgcgacaa ccgctccggg tgccgccatt actgcaaccg
    88921 aaaggaagta gaaaacatat ataagtactc ttgaggatat tgttccgaat accctttgga
    88981 taagtatatt acatgcggta cacagatatg agaaagacaa tcttgcaagt attagtacaa
    89041 ccacatcgaa tggtgtcagt tttaagataa acgcaagtgc tatgtataat atagccgctt
    89101 ccactaagaa tccggcaaat gactgcgcta tgttgtacaa tatctttttg aaagatgttt
    89161 ccggcaccag gtatatataa ggtttgccga gctccttgga gaaacgaccc atcttgaccg
    89221 atatcatctg catgtacgta gtgtatgcga aaatcggacc tattccgtat tctctcataa
    89281 ataccgcaaa tataatggaa acaatcatga acactatgcc ggccggttcc aatataaatc
    89341 ttcttgctct tctttcctct aatttgtgct tgtaataaaa agcacttgct ccttcacctt
    89401 tggaaagacc ggttttaccc acttttacct tgccggttgt ttccattatc ttgccttctt
    89461 tctgtgccga tgcaagcgaa aactgctctt cacttgtttt taaaacatcc tcatagtagt
    89521 cctgctttgc ataagaaagc aaaattgtca tgcccgctat atatgcaacg gttaaaaccg
    89581 aaccgagcag tatggcggaa atatctttca gcaatatgcc gttaagtatt ccgcctatcc
    89641 atcctccccc gggcataagt cgcaaaagta tactgtttcc ggcagttaca agattggtta
    89701 ctacattatc catatcctta agtaaaaata tgaccagtcc tattgcatat gcaatgatta
    89761 taccgaagaa aataactgcg gctatccttt ttttcttatc gtcgtaactg gtaaaagaat
    89821 atataaccat tgctgtcagt tgtgcaacaa ataccgataa tgagtaaccc aacaatataa
    89881 taataagttg caggaaattt attccgtaca aaatgtgcag ccatgagtat tgatataata
    89941 taaaaacacc taagagtaac gaagtaccca gttgctggaa taatccgtaa aacaacaccc
    90001 ttctcggatg tatgggtgac gtgaataaga gattcacatc cggcatcttg aacatagttg
    90061 ctccgctttt aaagccgtta taggcaatga gactgaacat aataatatac aaagccgtaa
    90121 gaccggctgt taattcccga ggatcacgga ataccgtatt ttcatccaca cgctttttcc
    90181 ccgatattgc ggtaaccact attaacgcta tcatgataac ggcatatatc aagcgagccg
    90241 gtttcttgaa cagttcctta atattatttt ttaactttat agccagaagg tatgtaattg
    90301 cgtttttttt catgcttctt cactcttttt ttcggtaatc ctgaaaaaca gttcttccag
    90361 actttccttg tcgtctccgg aaacgctttc atttaatttc gtagccgcaa atttcccgtc
    90421 catcattata tgagcgacat cccaataatc ttcaacgctg tcaatcatat gggtacttat
    90481 cagtattgtt cttccgtctt gcttcaattc ctgaaatata gcttttataa ccttaatggc
    90541 atgaggatcc agacctacta aaggttcatc aaatacaata acctcgggtt tgtgtaccaa
    90601 tgcacagcat atggataatt tctgctgcat tcctttggac aattccttgc ccaattttga
    90661 tttcttatcc cacagttcaa accgctccaa aagacttctc tcatattcgt catcttttat
    90721 cctatatgct ctggcaataa actccagatg ctcttccacg gtcagaagat catataccgc
    90781 aggaacttcc ggcacatacc ccaataaccg ctttgcttca atggatttgt tatcgtagcc
    90841 gtctaacttt atctcaccct tgaaacgcaa cagaccgctt atgcatttaa tgattgtgga
    90901 tttaccggca ccgttgggac ctaaaagaat tgctatctgt ccgccattga cagagaaaga
    90961 tatatcatca tttgccacca cttttccata ctttttcgtt acattcaata cttccagcat
    91021 tttcttcacc ttttatgtat atttaaaatc aaatttatcc tactctatga gatattttat
    91081 cactactgat acatagaaaa atcaattttc tgcaaattat tgtgttgctt gagttaaatg
    91141 tatatataca agttcaaaag aacgataaat tgcagaaaaa aacgtgaatt taaaggagag
    91201 ccttccggct ctctttcaac attagttttc tatgtttttt tccgtagtgt ttctatcttc
    91261 cgtatatgct gttggtcttt gaagaagcat aaacggacgt tataaggtcg acggccttca
    91321 ttccgtcttc cagtgaaaca taaggtgctc tcttttccag aagagcctct gtgaaatcgg
    91381 taagttgttt cacatgtccg ccggctgtaa taatatggct gcttgccgct tctccctgtg
    91441 ctatatcatc agccgaatac tctttctcgc cttctatgta aagacgtgca atatgctcct
    91501 cttcaagaag tattgtaccc ttggtgccgt gaatctctat tgttctgttg catgccggta
    91561 ccattgatgt ggacgcaatt attgttccca agcaaccgtt cctgaattcc acaaccgcaa
    91621 ccgcattatc ttctacctct attttgtgtg tcaaagtgcg tatgtagccg taaacattct
    91681 ttacttctcc tataaaataa agaagcgcat ccacaccgtg aataccttga ttcatcattg
    91741 caccgccgcc gtcaagttcc caggtgcctc tccatttacc ggaatcatag tattcctgcg
    91801 atctgtggaa tttcatggta aggttggcca ttataagatc accgagtctt ttatcctcaa
    91861 tcatgctttt tactttcctt attccgtcat aaaaccttaa ctgtgaaatt acactgccgg
    91921 tcatgccgtt tttcgcaaaa gcttgtacta cctgcttagc actttccgtg gtcaaggcaa
    91981 gcggtttctc aataatagcg tgcttcttat gctgtgccgc acttatggct ataggtgcat
    92041 gatatccgct cggagtacat acggttacgg catcaatctc ctcacttgat aaaagatgct
    92101 cattgctgtc aaagctttta atgccgtatt tttcggcaaa aggcacaggt tcctgcggtg
    92161 aacaaccgct gactcccaca agtttggctc cttttatctg cagaagggct tctgcatgag
    92221 tatgtgctat acccccgcat cctattattc cgtatcttat aatctcttta ctcatttcgc
    92281 tcctttgctc ttttcttttt tatcggtata gaagaaccat cttccgcttt ccgttcctct
    92341 cttctcatca cctgcaagca attctttgta attcaaagcc accttcttga aagcgtttgc
    92401 gtacatctct atgcattccg gcatatagtg tttaaaccac ggtattgaat atatctgaac
    92461 atgttgcgat accgttatac cttcatccag tttacgcaca tccctgtcgg taaatgccac
    92521 tcttgccggc ttatcatagt tcaatccgtt gtagtcttta aatatatcat gcagatgcaa
    92581 agccctgttt ccgcccgctc tggctgcaaa accttccgcc cgtaccgctt cacagaatcg
    92641 ctctacggaa agtccttcca gttcttccgg acagtataat ccgtggggag cataccatcc
    92701 tgccatattg gagccgtccg actcgtccac catatgggct ctgattcccg gaactccctc
    92761 cagaagactc cagaaatact tcattgcttt ccttatctcg gcagttctct catcataata
    92821 tttcagttgc acccttccca ttgcggcaca catctggttt actctcccct taactccgcc
    92881 caaaggcatg gaagcatatt ttaaaagata atcggtggat atgtacttgg aattgtttct
    92941 ttcataatgg caatatgcca ttgcacggtc atacatttcc ttgtcgtcgg ttacgagaat
    93001 acccatttca cctgtcgcaa aagatttccc gctcattgcg gacattgcgg ccacatgacc
    93061 gaatgtaccc aacattctgc ctttatacat gccgccctga gcatgggata catcctctat
    93121 cacataaatg ttatgtttct ttgcaatagc cataatgggg tccatgtcgg cgggatgtga
    93181 aagataatgc acaaccatta ttgctttggt tctcggtcct atgcatcgct ctatatcatc
    93241 ggggtcgata catagtgtcc tgggatgtat atcggcaaaa accactgttg cacctaaatt
    93301 aaacgcctga agacaggatg cccaataggt cttgctgggg catattattt catcgcccgc
    93361 tcctatctta catgcgtaca ttgcagcttg aagcgacatt gtgccgttgc aatatgcgat
    93421 ggcatatttt ctcttctgcc atttggcaaa ctctttttcg aatttctccg ttacatccag
    93481 tccgctcatt gcacctcttc tcaaaacatc cagaacggca tcttcatctt ccttggtcac
    93541 tatgggccac ttgaaaagcg aatccggcac cttcgttttg tctattgccg gctttcctcc
    93601 gaatattgca agttcactca tttatgtttt cctcctcttg tatgaatact tctatattat
    93661 accctaacat ttttattgcg gtatttcttt gcactttcca cttatttatt gactgtttcc
    93721 attcataaat cgttttctgt attgtcccgg cgtcatgccg ataatctgtt tgaatgtgcg
    93781 gttaaaatgg tttacattgt taaatcccga ctcataacat atgtttataa ccaggtcatc
    93841 ggtgtgtttc agcatctccg aagcttttgc tatcctcagt cctgttaaat attccttgaa
    93901 tgttttggat gtaaggttct taaacagata accgaagaaa gagggagaca agtgaaactt
    93961 ctttgcaacg gcatcaatat tcaagtcctg cgtatagtgc tcttctatat atcgtatagc
    94021 tcttttgatt gcatcgttat gtctttcaaa aagctttgcc gatttgctgt tacttatctt
    94081 tttggtaaat tctcttccca ccatcactaa aagtttcaaa agaagcgagc ggattataag
    94141 gtcaaatccc ggctctctgt tttcatattc cctgagtatt tcgttaaaaa tattttccgt
    94201 ttcacactgt attttgccta ccagattaaa tctcggtctg accgaatgct cttccactaa
    94261 aaaaggttct atatatgcga aatcccagaa cgactcgcct tttttcaaat cattaagaaa
    94321 ttcgttaata aatttgggat caaattcaaa ttccactatt tcggtatcgc ttactcccgc
    94381 gggttgtatc tgatgcggaa cattcggagg aatgacaaaa atatctcctt ttacaaggtc
    94441 atattttttg ttatgtatga tatgaacggc ttttccgctg tatacatagt ttatctgtat
    94501 gcatttatgt gcatgcagat aatgtgttcc gttttccggt cggtattttt tgacataaaa
    94561 catcaaatgt ccgtcaggat tgtcttctat tctgaaaacc ggtatgtctt ttagattgct
    94621 tttatccgtc atcaatcaca tctcacaaca agtactacat ttcccactgc cgtgatatca
    94681 tttattacct tttcttcatc gacttttctc ggtatgccta ttacaatact caacccgata
    94741 ttgccgtatg tgccgctttt tgcactctgc acttcaacat tatctatggt gtatcccgct
    94801 ttttcaatat cctttataac ggtatttatg ttcctgctgc cggtaatctc cagataaaca
    94861 tccatcttta tccttctgga cccgaagttg cctaagtaag ccattactat tgcaagtact
    94921 ataacgcata tgatagccgc ttcataaaat cccgctccta ttgctaaacc tacagcagca
    94981 atggtccaaa gccccgcagc ggtggtcagt ccctttacat gggtatgacc ggtaacaaga
    95041 atggttccta cccccaaaaa tcctatgccg gaaagtacct gagcggagag tcgcatggga
    95101 tcggtatcaa aacccaatat atccgacatg aatattcctg tcattgcggt cagggccgac
    95161 cctatgcata ctatgatatg tgtccgaaac ccggctgaac ggccgtgcct ttccctctgc
    95221 attcctataa gtccgccgca aagaaaagcc aacacaatcc ttataatcat ggtcacaaaa
    95281 ttaagttctc tgagataaga aatcacattg tccatgtcag acctcctttc tttcagtcaa
    95341 gattcttcat ccatccctca gaagatgcgt cgctcggcat gcgccagtcc cctcttggag
    95401 aaagtgcaac agaccctatt ttaggtccgt tgggactgca gcttctctta aactgatgca
    95461 ggaaaaatct tcttaaaaat atcttcagcc attttttaat ctcatcggcg gaataattat
    95521 ctttaaacgc atatgttgca agcataagta tttttttcgg tgtaaagccc cagcggagga
    95581 aatagtataa aaagaaatca tgcagttcgt aaggtcctac aatgctttcg gtcttttgta
    95641 taatttcacc gtttactccg ggtataagtt cgggacttat gggtgtatcc agaatatcat
    95701 acagaacagc tttcagtttg ctttccgaac ggtcagcaat atatcggata atatgtctca
    95761 ccaatgtttt gggcactgaa gtatttacat tgtacatgga catgtggtct ccgttgtatg
    95821 tactccagcc gattgcctgt tccgaaagac tgccggttcc tataaccata ccgtttgtca
    95881 tattggaaat atccatcaat acctgcgttc tttcccgtgc ctgtgcattt tcatatgcgg
    95941 catcggttgt tactccgtca tgccctatat ccgctaaatg cctttttacc gaggcggtaa
    96001 tgtttattac tctgaaatcc gtattaagaa tcctgctcag tttgcttgcg ttatcctttg
    96061 ttctttttgt ggtgccgaaa cacggcatgg atatacacac tatatccgaa ggagaccttc
    96121 ccatatcctt aaatgctcta tgtgtgacaa gcaatgcaag agtggaatcc atacctcccg
    96181 atatacctat aaccgcggtt ttgcagtttg tgtgttccat tctcttttta agacctgttg
    96241 cctgtatgtt caatatcagg tcacagcgct tttccatcgt gtcgttgtct tccggtatga
    96301 aaggatgttt ttttattgtc cgggatatcc gacattcaac aggcttcatt gtaaacggta
    96361 taatcttata gccgctatca tgctgttccg ctacgggtaa ccggctcttt ctgtcgtgag
    96421 acagacgttc cagatctatc tcggtttcaa tgtattcacc ggtaaaaggc agagcttcgg
    96481 aaagaaccgt tccgttttcc gctatcatct tatgaccgga aaaaaccatg tccgtagtgg
    96541 attcccgatc ggatgcattg ctgaagatgt aagcacatat actttttctt gattgcgctt
    96601 caatgagcgt tcttctgtaa ccggctttat ccagaagttc attgtttgcc gaaggatttg
    96661 ctataaccgc ggctccgttc attgcgtgca gagaaccggg atttaccgga gcaaacaaat
    96721 cttcacctat ttctatagca agtgaaaaat cgttcataag catattacgg aatatgagcc
    96781 ttgttccgaa cggtaccgac gtaccgttaa tatctattac ggagttctcc ttcggtgcac
    96841 tcctgaatat acgcatctca taatattcgt tatagctggg aagatgggtt ttgggaacaa
    96901 tacccagtat atttccgtta tacactactg cggcacagtt atacagataa ccttccgcct
    96961 taaccggaag tcccgtaacg ataagcattt ctttgctttc cgatgctttg acaatatcta
    97021 aaagaccggt taacgcttga tcgataagaa gcttatgtga aaaaagatcg tcacaggtat
    97081 atcccgttat attaagttcg ggaaatacca gaagttgaac atttctgtca tatgcctttt
    97141 ttattgtttt aataatgccg ttgcaattgt acttacaatc cgcaactctt aatgatggac
    97201 ttgccgccgc cacttttata aatccgtaat gcattttaat acctcttctt atatagatta
    97261 tacactgttt agggaaaaat taacatattt gtgttatgtt gataagcctt ttggtatgag
    97321 gaaacataat tatccgtttt gtaagtcatc ggcaagtaac ttatcattta atatccgaca
    97381 gagcagtccg actaaaattt gagctatgcc gcttattgcc agcacaatgg gaatacccgc
    97441 caatttgaag ccgaaaatgt ccacaacatt attccctata cgccaaaagc ataaagtgcc
    97501 caacatcata ctaaagaaac aggacatatg tactgccagg ttatagaagg aaatatgatt
    97561 tgttctgtcg ctttccggaa tattgtcatc caacaacgaa gcgataacgg aatttatgat
    97621 aactccgagc acatgctgca ttaatcgtac aataacccaa agccatatat agttttccga
    97681 tgtgacaaaa gcatacatga tatatgtagg tccctgcagc agtaatgcat aaccgagtat
    97741 cctgtaccgg gaattcttat taagagactt tgtcagcgcc ttaccgaaaa agacatgaat
    97801 cagaaagtat atggcattaa taaggttgat ttcccaatag cttaaaccta cacttttaag
    97861 caaaaagccg tttatggcgg cacttgtcag gttaaatccc aaatagtaaa gggctactat
    97921 aagaaccgtc cacagaaatt ttttattctt taaaggcaga accaacatat cttttatctt
    97981 tgtttttttt ctgcttggtt catagggata ttccttaggt atagccaata caactatatt
    98041 caatacggct aacgcaaatg cgatatatcg gaatgtggta atgatttcta tctgttcgga
    98101 actgcccgca aaagaatcta ttatcaatga aacgattaaa accacggttt gagtaaaaaa
    98161 accgcttatt ccggaggtta aggtataata gtccgctctt acatcattgg gaagataagg
    98221 tatatgccag gcggaataac cggaatcgat tattgcggat attgcagtgg atattgttac
    98281 cagcagtgtg aatcctacta ctctgccagt ctgcgacgta attaaatcgg gaagcagtgt
    98341 cacccccaga atattgaaca cattagccaa tacggctaaa accgtgagga aaacttttct
    98401 tctgcgaagc ctttccaaga ttaacggtga gaatatggcc aacagccttg ttatcatggg
    98461 aacaaagata attatcccta tgttaacggt attcattccg aagcatatta gaaagccgat
    98521 ataaaatgct ccgccgctta actgcgtagc taatgatgaa agtatggatg atgctaataa
    98581 acagtctctt cccttagctt tttcgtcgtt cagattaaat gtttttctaa aggaggaatt
    98641 ctgccatttt gtctttattg catgtaggaa ctcgctcata aacccctcaa tccgctgatg
    98701 ttgtgagtac tacaatgcag attatatcaa ttgaacggga atattggcaa taactgattg
    98761 tgtgttcatc ttctcaataa ttcatcggga agcagcttat catttaactt ccgggagagt
    98821 agtcccaccg ctatctgcat tatgccgctt acggcaagca gaataggcac tgccgccatt
    98881 tggaatccga aaaagttaag cgtattttct cccataactg aaagaaagaa tgtaccgaac
    98941 atcatactca agaaaatgga catattaact gctatgctat agaaggcaat atagtttgta
    99001 cgatccttgt ccggaaggtt gacatacagc attgaggcgg ttacggcatt tacaataaca
    99061 cccagtacat gctgtattaa tcttattaaa aaccaaagcc agatatagtt ttcaggtgta
    99121 acaaaagcat acataatgaa tgtcggtccc tgcataaaca ttgtataagc aagtgtcttg
    99181 aaccaggaat gcttcttaag aaactttttc cacatcttac cgaaaaagat aaagaacatg
    99241 aagtataccg cattgacaag attaatctgt gtataggtta tacccacatc ctgaagtaaa
    99301 aagatgttta tagaggcggt tgtcagattt attgccatat agtaaaaggc taaaatgata
    99361 atggtccaca aaaatttctt attacgaaag ggcaacacga atatatcgct tatctttatt
    99421 ttgtcgccgc ttcgttcata agagtattct ttaggtatag ccaatataag tatgcgtatt
    99481 accgccaaca ccaatgccgc ataacggaat cccgtcatta tttccaattg cccgggactg
    99541 cccgcaaagg agtctaccac caatgaaaga accagtgcaa taccctgcgc taaaaaactg
    99601 ttgattccgg aagtcagtgt gaaatagtcc gcccttatat cgtcaggaag atacggtgca
    99661 tgccaggccg aataaccggg atcgattatt gcggatattg ctgaagaggt tgttaccaat
    99721 aatgtaaagc ctattactct tcccgcttcc gatgtaataa tatcgggcag caatgttaca
    99781 cccaatatgt ttattacatt ggctaaaata ctcattgcag taagaaaaac ttttctcttt
    99841 cggaatcttt ccaaaataaa aggagagaat atcgacaaca gctttgttat tatgggaaca
    99901 aaggttatta tccctatatt aacaatgttt attccgaaac ttaagagaaa acctgtatag
    99961 aatattccgc cgctcagctg tgtcactaat gataaaagaa gagatgatgc caaaaaacaa
   100021 cttcttcctt tagccttttc ttcatgcaga ttaaatgttc ttacgaaagc cgaattccgc
   100081 cactttatct ttatcgcatg caggtactcg ttcataaatt cctcaaccgg ttaatgcctg
   100141 tgcattctat aatactgatt ataccaagtc aacgggaata ttggtaacta ttatctcttc
   100201 gataagtcat cgggaagcag cttatcatta aacttccagg aaagaatccc tacaataacc
   100261 tccgctatac cggttatggc aagcaatacg ggaacacccg ccattttaaa accgaaaaca
   100321 ttcaagacat tgtctcccat ccatgaaagg aacatggtgc ccagcatcat acttaggaaa
   100381 gaggacatat gtattactat gttatagaaa gaaacataat atgtcctgtc cgtgtccgga
   100441 agatttacat acagcattga agcaattacc gtattggaga ctacaccgag cacatgctgc
   100501 aacagtctta ccgtaaacca gagccaggta tagttttccg ctgtgacaaa agcatacata
   100561 atgaatgtcg gtccctgtaa aagcattgca attgccagag tcttaaacca ggaatatttt
   100621 ttaagaacct ttttccacat ctttccgaaa aagataaaaa acatgaaata tacggcattg
   100681 ataaggttaa tctgagaata ggttacacct acatcctgaa gcaaaaagat gttaatggaa
   100741 gcatttgtaa gacttgctcc caaatagtaa aagccaatca gaagaaccgt ccacagaaat
   100801 tttttactct taaaaggcag aatgaatatg ttacttaatt ttatcttgct gtcgctttgt
   100861 tcataaggat actctttagg tatagccaat accagtatat gcattatagc caacaccaat
   100921 gctgcataac ggaatcccgt tattatttcc aattgcctgg ggctgcccgc aaaggagtct
   100981 gctaccaatg aaagaatcaa taccacacct tgcgttacaa aactgtttac tccggagctt
   101041 aatgtgaaat agtcgcccct tatctcattg ggaagataag gtgcatgcca ggcggaatag
   101101 ccgggatcga ttattgcgga tattgctgaa gatattgtta ccaacaatgt aaatcctatt
   101161 actcttcccg tttccgatgt aataatatca ggcagcagag ttacacctaa tatgtttatt
   101221 atgttggcca atatacccat taccgtaaga aaaacttttc ttgagcggaa tcgctctaaa
   101281 ataagaggcg agaacatcga tagtagcctt gttattgtgg gaacaaaggt tattatccct
   101341 atattaacga tatttattcc gaaacttaag agaaaaccgg tataaaacac tcctccgctt
   101401 aactgtgcag ctaaagagga gagtatggac gaagctaaaa aacaacttct tcctttagcc
   101461 ttttcgtcag tcaggttaaa tgttcttgca aaagcggaat tccgccactt tatctttatt
   101521 gcatgcagat actcgttcat atatccctca atcagctaat attctgagta ttctataata
   101581 ctgattatat caagtcaatg agtacattgg taacaattat ggaaattagg aaattataaa
   101641 actatgaata ccgggaacct ttgtgtacaa aatatatttg caaataacct atagacaact
   101701 atatgtttgt gatataataa aagtctaata gtactaagta ctaaatagaa aggagaaata
   101761 caatgaaaaa agatcagaac agatttttta tctgcagacg ttgcggaaat atgataggaa
   101821 tgatttataa ctcgggtgtg cccattgtct gttgcggagt gaatatggag caattggttg
   101881 caaatacaac cgacggtgca aatgaaaaac acttacctgt aataaaacag aacggtaaca
   101941 atgttgttgt ggaagtatcc accgtagttc atcccatggt aaaagaacac tatatcatgt
   102001 ggatttatct ggaaacggca aaaggcggac aacgtaaatc ccttcttcca ggggaaaaac
   102061 ccgtggcaac gtttgcaatc accgatgacg acaaggtaat tgcggcatat gaatactgca
   102121 atttgcacgg actgtggaaa actgccgtaa aatagtgtat agtcataaat ataattgtgg
   102181 aggaggtttt ttccgtgaaa aaatatgttt gcatggtatg cggttatgtt tatgatgaaa
   102241 aagaaggcga tccggataac ggtgtagatc ccggcacaaa atgggaggat gttccggaag
   102301 actggacatg cccgatatgc ggagtttcca aagaggagtt tgaagaagaa taatataaac
   102361 atcaactcga agaaagcagc acatgctgct ttctttttta tcgagcctgc taagaatata
   102421 atcatattat cattatacgg tttgttaata aacataactt caaaccgtta gatgataatg
   102481 tataatagtg acatggtgga tacaagatga gaaaaataac tgtagcaggt tcaaagaaat
   102541 atgacataat aatcgataaa aacattctgt cggaagcata tcaatatatt aagcctttct
   102601 taacaggtaa tattgtctat atcgtaaccg atgacatagt ggattcgcta tacggctcta
   102661 tattaaccga ctctttggaa agcaacgggc tgtcggtatt caggtatatt attccgcacg
   102721 gggagcaatc caaaaacgcc gacaattaca tagcggtact caatgatatg gcaagcaaag
   102781 gagtttccag atcggatatg cttatcgctt tcggtggcgg cattcccggt gacataggcg
   102841 gctttgctgc ggctacatac ctgcgcggta taccgctttt acacattccc accacattgc
   102901 ttgcaacagt ggattcctca ataggaggaa aaacgggaat taatcttaaa gaaggcaaaa
   102961 acctcgcagg tgctttttat cagccgcaag cggtaatatg cgacctttca ttattggaca
   103021 ctttgccgga tgaagtattt aaagacggat atgccgagat tataaaatac gggatattat
   103081 cgggcaatca gctttttaac cgtataaaaa aacctctaaa aagcgatatc gaaagtatag
   103141 tgtacgaatg tataaagata aaaagagaca ttgtacaaaa agatgaatcg gatatcggta
   103201 tacgacaact tcttaatctg ggacacacga tagctcatgc tgttgaactt ctgagcggtt
   103261 atacaatttc gcacggtgct gcggttgcaa agggactgca tttaatatct aaaatttcct
   103321 atacacaagg gttatgcacc aaggaagttt taacaaagat aacggatatg ctgaacctcc
   103381 acggttttaa taccgatttg ttatacggcg caaaagatat cgcaaacgca ataacggcgg
   103441 ataagaaaag aagcagcggc cacataaatc tgatacttat acgggacata ggtaaatgca
   103501 ctatagaaaa aaccgatatg tcagatttga ccgatataat acaaaccgca acggaatata
   103561 gaggttgaaa tggatatatc ggttacagta aagaatcccg ggggtaccgt aaaagcgatt
   103621 acatcaaaat cacatgcaca caggcatctc atatgttcgg cattgtcaga tgtacctaac
   103681 tttgttgaag taaccgacac ctcttccgat attgatgcta ccgttcaatg ccttaactca
   103741 ttgggtgcaa acattaccgc tgaaaaaaac ggttattata taaacccgat aaaaaccgta
   103801 agaataaatg cccttttgga ctgcaatgaa tcggcctcca cttttcgttt ccttcttccg
   103861 gttacctgtg cattgggagc aaagtcttcc tttattatga aaaaagggtt atctaaaaga
   103921 cccataacac ctttatataa tcagcttatc ggaaaaggct gcacattatc tttagaagga
   103981 agcgtacctt tccttaccga agggcagctt aaaagcggta tatatcaatt acccggtgat
   104041 gtgtcttctc aatatatatc cggtcttctt ctggcacttc ctcttctgaa gaataaaagc
   104101 gctatcctgc taacctccga ccttcagtct tcgggatatg ttgatatgac tgttgatgtc
   104161 atgaatcaat acaatgtaaa gattattaaa acagagaaag gttttgaaat acccggcaat
   104221 gctgcttata caccgacagg aaatatgagt attgaaaaag actggtccaa tgcggcattc
   104281 ttcctttgtg ccggagcttt ctctgaaaga gcaattaccg ttaccggatt gaatatacac
   104341 tcccgacaaa ccgacaaaac aattatcgat attcttaaaa caacgggagc atatgttaca
   104401 gttaataaag atactgtaac cgtcaaaaag aacaggttaa atgccaccga tattgatgca
   104461 tcacaaatac ccgaccttgt ccccgtactt gctcttttgg cagctgtttc cgaaggaaca
   104521 accaatatat tcaatgcaaa aagacttcaa ctaaaagaaa gcaacagatt atcttccact
   104581 tgtgatatgc ttaaaacgtt aggtgcggat ataacggaaa agcccgattc cctggttata
   104641 acaggaaaag aaaagttgaa cggcggaaca gtttgttccc ataatgacca cagaattgcc
   104701 atgaccgcgg caatagcatc tattgtgtgc gaaaatgccg taataatcaa agaggcgcat
   104761 gcggtaaaca aatcatatcc cgcctttttc cgggattttg catccatagg cggtataacg
   104821 gaaaggattt agtaaaatgt catcggtatt cggaaaaaac ataacagtca ctatattcgg
   104881 acaatctcat tcaaaaggta taggtgtggt tatcgacaat ctgcctgcag gtttttcaat
   104941 agatatggag caactcaccg cttttatgca aagaagaagt gccatagata aaccgctctc
   105001 cacaaaaagg aaagaaaagg atgaaataga gtttctttca ggactggtgg gaaatactac
   105061 atgcggcgcc ccgatatgtg caattattta caacaaagat ataaatcccg aagactaccg
   105121 atcctttaag gatatcccca gaccttctca tgccgattat acggctcata taaaatacaa
   105181 aggttttcag gacacttcgg gaggaggaca cttctcggga agactgaccg cacctttatg
   105241 tatagcggga ggtatctgtc ttcagatact ggaaaagtac ggtataagca tacatgcaca
   105301 cataaaaaag atatacgata cggaagatat atctttcaat cctgcagaag ataacagaga
   105361 gaatattgaa aaactcttaa acaatgactt tcccgttata gacaagcaaa aaggcatact
   105421 catgcaggag cgcattcgta cagcatcaca acagaatgat tcgttaggcg gaataattga
   105481 atgtgcggta aataaccttc ccgcaggaat aggcgagtcc atgtttgacg gtctggaaaa
   105541 caacatttcc aaggccgtgt ttgcaatacc cgcagtaaaa ggaatagagt tcggtaacgg
   105601 ttttgattgc gccaatctga aaggctccga gaacaatgat cctttctata tcaaagacag
   105661 caaaattctt acacggacaa ataaccacgg cggcatattg ggaggcataa gctccggaat
   105721 gccgttgata ttcagagtgg tcgtaaagcc cgctccgtca ataggtattg agcaggaaag
   105781 tgtcaatatg acaaacaata acagtgagca aataaaggta ggcggaagac atgatgcatg
   105841 tatcgtgccc agagcggttg cctgtgtgga agcggcggct gcaatagcaa ttttagacat
   105901 gtatgcatca tacagaatat gggaataatt taaaaattat ataaagggat aaaatgaatt
   105961 tagacgattt gagaaaagaa atagatagaa ttgatgataa acttttacaa ttatttcagg
   106021 aaagaatggg cattgtaaaa gacattgcgg agtataaaaa agcacacagc atacccatac
   106081 ttaacagtga attggaaaaa gccaaacttt caaatattat ctccaagacg gacgaagagt
   106141 tggtctccta tacacaaata ctcttcaata cattgtttga tacaagcaaa tcttatcagg
   106201 aaaagctgaa tatttctcag ccggctgcgc tcggatattc cgataagaag ttcggtttat
   106261 tgggtgccga attacagcac agttactccc cgcttataca ttcccatctg ggcgattatg
   106321 aatacgggct ttatgaaaaa cagtccgatc agctttatga ttttgtacat aacagcaacc
   106381 ttgacggttt taacgtaacg ataccctata aaaaagatat tatgaagtac tgtaatatac
   106441 tgacacctat cgcaaaaaga accggcagtg tcaatacggt tatagggaaa gcaaacaatc
   106501 ttacaggaga taataccgat tactacggtt ttagatatat gattgaaaga agcggcataa
   106561 atccggaagg taaaaagatt ctccttctgg gtgacggagg ctccgctgcc acaatcaaag
   106621 cggcaataca ggacatgaac gcaggctgtc tcgtaacggt atccagaagc ggtcaggaaa
   106681 attacggaaa tataagcaat cactatgaca gcgacataat tattaactcc acaccggtag
   106741 gaatgtaccc tgacaacggc aaaaccgtta tagatatctc ctctttcaaa aaatgtattg
   106801 ctcttttcga tctcatatac aatcccgctc aaacaagatt gatgtatgaa gcatccaaac
   106861 tcggcataaa agcatataac ggactctcca tgcttgcggc acaggcaaag aaagccgctg
   106921 aactatttac cgaatccgtg atcgatgatt ccgtcattga aagagtagtt gaaaaggtag
   106981 ctaagaaaac cgggaacata gtactcatag gaatgccggg aagcggaaag agtactgtgg
   107041 gaaaagctct tgcagaactg actgaaagag cctttataga taccgatgag gtaatagccg
   107101 caacatataa taagagtgca tcggacatta taaaagacaa aggagaagtt ttcttcagag
   107161 acattgaaac aaaggtgctg gaaaaagtaa ctaaacaaag caactgtgta atatctacag
   107221 gcggcggcgt tgtaacccga gaaagaaacc gatatctctt acgccagaac ggtattgtcg
   107281 ttttcctgaa aagagattta tcactgcttt gcacactcga cagaccgctg tcgcagcaaa
   107341 acactttgga agagctttac aaagcaagaa agaataatta tctcggctgg tcggattatc
   107401 aaatagactg tgacgataca gataaagcac atttaaaaat atatgaggtg ttaggcttat
   107461 gaaactttta gttataaacg gacccaatat gaatatgctg ggtatcagag aaccggaaat
   107521 atacggtcat gcaacatatg cggatttagt aagatacata aaggatatct gtacgcaaaa
   107581 aaatattcaa tgtgagattt ttcaatccaa ccatgaaggt gatatcatcg ataaaataca
   107641 ggatgcatat atgatttccg atgccatagt cataaacccc gcagcatatt cccatacaag
   107701 tatagccatt gccgatgcaa taaaatccgt ggggcttccg gctgtggaag tgcatttaag
   107761 cgatattaca aaacgtgaag atttccggaa acattcctac actgcaaatg tatgcataaa
   107821 aaccgttacg ggaaaaggtt ttgacggata taaagaagcc atattgcttt tgaaggatgc
   107881 tatcgacaga tgctgattaa gtaattattc ttattatctt ttacgaagaa atcggcagca
   107941 catgccgccg actacttatg ctttcttcta taaccaaatt aatcctattt attcttatcc
   108001 tttatatcca caaccacctg ctgtaaaggt atctcaatcc cttgctcatc aaatgatttc
   108061 tttacgtttt cgttcatgtc aaaatatacc gtccaataat cgtcggcttt gcaccatgct
   108121 cttaatacat acacaagtga attaggacca tgagcataaa ctccggacgt tacagacgga
   108181 tctttaagaa ctaaaggatg tttctccgct actgaagtca aaacactttt taccttatct
   108241 atgtcgtttt tatatgaaac tttgtactct atatccacgc gtcttaactc ttttgcactg
   108301 aagttggtta tggaagcggt agtcatggta ctgttgggaa cagttacaag tttattatct
   108361 tttgtcagca gccttgtata aaatatggtt attgaatcga cataacccat atgcgttccg
   108421 ttgtctacaa aatctcctac cttgaaagga cgaaatacca gtatcattat acctccggca
   108481 aaattggaca gtgctccctg taatgccaat cctattgcca gtccggcaga agcaagcacc
   108541 gctattattg atgtcagcgg aacacccagt attcctatca cggagataat aagaagcact
   108601 ttcaatgtaa tagccagagc gctttttaaa aatgtctgta ccgttacgtc aattttattg
   108661 aatcctttgg atttcactat aaacttcaca aggtatttga taagcagaag acctacaatc
   108721 agtaccgcta ttgccagcag tatcctcaag cccgccgtag cagatgcttc aagcagagta
   108781 tcccatagtt tgcttaatgt ttcattcatg ttttttacct ctctttgcta taaactatta
   108841 tatctcaata ctgtcggaaa gtgtcataaa ccgattcatt tatacctcat tatctgcttt
   108901 tgcatattat atcatcttca ttcttacttc aataaactta ttcttccctc tgcatataac
   108961 aaacttacct tttaatctga cactaaagtc attaacagtt atgccgtcaa ttttaatgcc
   109021 tctgcccgct atcagtcttc tggcttccga gttggatgtt gctaaacctg tttttacaag
   109081 aacatcaata atgcccgtct catactcctc aatatgaaat acatctatat tgtccggaat
   109141 tgcctttgcg gcaatactgt tatatctgtc ttccgccgca ttaatacagt ctcttccgtg
   109201 gtacatctcc actatctttc ttgcatatac tttatgggct tctcttatat cctcagaaat
   109261 aatatcggta tactccgctt cacaaatatc cgttgtaagt ctaaaatagt cagtaagcaa
   109321 tccgtcggga atccgcatac acttttcata cattatctcc gccgactcat ctatacctat
   109381 atagttatcc aaagatttac tcatcttctc cactccgtcc aatcccgcaa taagcgggaa
   109441 agtcataact acctgaggaa tctgcccgta atccttttgc agttctctgc ccaccagaag
   109501 attaaatgtt tgatcggttc ctcctatttc aatatcagca ttaaccgcaa ccgaatcata
   109561 cccctgtata agagggtata aaagctcatg aatacctatc ggttgattat tcgaatacct
   109621 tgcggaaaaa tcctctcttt ccattatcct tgcaagagta tacttcccgg ccaattcaat
   109681 aacatcggca aacctcatct ttcccagcca ttccgagttg tatactatct ctgttttcga
   109741 cggatccaat atctttgtta cctgctcaaa atatgtttga ccgttctttc cggtttcttc
   109801 gtaagacagc tgacttcttg tcttgcttct gcccgacgga tctcctatca tgccggtaaa
   109861 atcgcctatc acaaatatga tatgatgtcc tgcctgctgc aacactttca gcattctcaa
   109921 aggcaccgtg tgtcccagat gcaaatccgg tctggacgga tcggcaccga actttatctt
   109981 cagggatttc ccgctgttca gttttgtgcg aagttcttct tcattgaaaa cattgaccgc
   110041 atttttcatt agaatttcca ttgtttttcc attattcata ccggtttctc cttacaatca
   110101 aaaaagactc aagcgtttgc ctgagcctaa tcattaattg tttctacaaa agaattacga
   110161 caataacagc ccaagcctat ttaagtagta atagttgtaa taatacatat ttgaactgtg
   110221 ttgtctgttt actgccattt ggcgctcctt tttaatttat ccgaagtata tcaacaaatc
   110281 tttgtaatgt caataaatat cggtaatagt aatagtgcta tttaataaag ttcgtcctaa
   110341 tatcttttta ttgaggaaag cccaaaccat gccggcagag ccgcgaacgg attatgcttt
   110401 atatcatatt tcctgtttcc gtccgcttcg gcttctttaa tagttttgta aaaccataag
   110461 tcgtgtgccc cgggttctgc gtgaaattcc tcaagatgca gaatataaaa gccttggctg
   110521 ataacggcat taaatatatc cataattcgc caatggtttc ctgttgtatt gttgtacggc
   110581 tttttcactt ttatttttgc acctgaatcg tcaaacggtc gattgaaagg atgagtttca
   110641 aacataatat actttccctt cggcttcaac acacggttga agtttgaata catagcttgt
   110701 aggtcattta tccagatatg agtgccgttt gatgtgtaga caagatcaaa ttcactatcg
   110761 cacattccgt ctaacttcat agtgtcggct tgaataaatt caatattcca gcgttcattg
   110821 tcggcaatct tttttgcatt tcccagttgc tgctccgaaa tgtccgcaga gacaactgtt
   110881 gcaccgagca aatgaaaggc aaaaaccgct ccgttgtctc cgcttgacgg tacaagcacc
   110941 ttgatacctt tcatactgcc aaacgcattt tgaatcatat tacggacagg tacgggaaac
   111001 gcccaccacg ggtcggcttt tatccttatc gcattgttgt ttatgttata tgctctgtta
   111061 tagtactcat cggaaccgtc attccagaac tttttaatgc tgctttccat tatactcctc
   111121 cgcaagcata gcataacaaa gccggtcatg acgcacgccg ccgaaataga ttgccttgcg
   111181 ctcgcgtcct tccaagacaa atcccagctt ctccagcgac ttctttgctg aaatattgca
   111241 gtccaatgtg cctgccgtaa tgcgttcaag acccaggttg caaaaaccgt actcaagtat
   111301 cagcgagact gcctgcttgc cataacctct gttacggttt tccaacttct gtatgcccat
   111361 gccgatggaa caggaacggt tacgattatc gatattctgc agggcgatat ctcctatcac
   111421 agtaccgtca ttaagaaaga ttcccagtcg cacatgtgtc tttccctgtg ccgcctgtat
   111481 ctcctcaaac cacttgtcgg ctccttctac agaataaccg atgtaaagaa tatcggtagg
   111541 attctcaaaa tcgtattcgt tgtcctcaaa aagtttgcgg cagtctgcac gttccagcgt
   111601 tgctaaatat atggatttcc cctgtaattt aagcattttc ttttcctccg attaatgtga
   111661 ttacggtaaa gcctgtcata atcgccgttt ttaccgctca ctcattaata gtataacagt
   111721 actcatactc cattcccaat aggtttttct ttcccgcata ttttgatgta tcggatgccg
   111781 gtgcaaaata ctgctccaga tattccagca acggtgtaat gtcggcatcg cttcttgttt
   111841 ctataactat agacaggatg cactcgtttt tgaaaggctc ctttacatac ttcctgttat
   111901 acccggtata tgtaacatac accgtatcac tttcgtcagc ggtttcataa tagcctatta
   111961 ctttaaatcc gaatctcatt ctgcccgaag ggttgtcttc ttttttcggt tgctcaaaca
   112021 accttataat ctggtagtct atatcgggat ggttgcggac cgattcccat atcattttag
   112081 gcacaattgc atatcccgtt atgccgctgt ccaagtgatc cttgggttcc acatagtctt
   112141 cttttctgat aacaacaagg tcattttcgg tatagttggt ataaaatgtt atatcacaat
   112201 tttttttcat acttttaatt tctcttacac ttgtaacggc ttgatggtac caaggactat
   112261 ccataccgtg tgattggtag tattccgtat atttaacata aaaaccttcc aatccgtaac
   112321 ctccgaaatt aataccgtaa cagggaatct ttacatttag cgggtactta gaccaatcct
   112381 ctccttttac ccctgtgctt attattatgt ccgttatata ttgcgaatat ttatggtagt
   112441 cttcaatcaa tgcatcgccg tatacatgtg acatctgaag attatcgtta ccgtctattc
   112501 cggttacggt tattatattc ttttcaaaac cggaatcttc cgaaagattt atcggcagga
   112561 tatcaacatt atttacatac atatattgat aaggttcatt gaatttgttt attctttttt
   112621 catactgtga aagcacatta tagtctgcaa aatattcgct caggaaatat gagaatttta
   112681 caaggttcgc tttatcgttt acatcaatcc ccatgtagtc gatatgaccg acaatatctt
   112741 ctttccctcc cagcaccaca ttgctcaatc ctgaatagga aaagtacaaa gtgtcgtgtc
   112801 tgtgcttggt ttcatattcc cctattatgg tgaaatatgc cagaccgctt gtgccggaat
   112861 ttataaggaa caataccttt tcattatcgg ataattgaga atctatatgt tcgctaagag
   112921 tagagggaat tattacatag ttatgtaaac tctgttccag aaggtccgca gagtaacctt
   112981 ggtaaattgt gattttactg tccgctttat cagcagttaa accgcttaat gcagttatac
   113041 ctttacagta cttaattgat tctataactt tctttgtctc tccgctttcc ggatttttca
   113101 aaacctcctt acagttatac aaagtcagac ccgttttact tatttcatat ctgacggtgg
   113161 ttatgttgag tctttctatt tcctctctga acaccaacct tttatccaat aaagccttgg
   113221 aatatctgta cggcatattc agcggttcct gtgcatccga ccttttaata actatatcgc
   113281 tgttgaaagt tgtgacattg aaatgtccga tattgcaaaa aagtctgtcg cgaaaaagaa
   113341 acaacaatgt tgctacaacc gctaaaacga ttacagcgac agtaataacc aaacctgtct
   113401 ttttcttttt caccatacgc ttaatgccct caaatacctt cactaacgct gtttatttaa
   113461 atacaacaca agttacggaa taatgcgtgt taataattaa tccgcattgc ttttaattgt
   113521 atagcaatat tcataatccc tgcccaatgc atttttcttt cccgcatact ttgatgtatc
   113581 ggttgcaggt gcaaaatact gctccagata tccttgcaac acttttatat ccgtatcatc
   113641 ttgtgcccat aaggttacgg actcgatgtg ttcgttctca aacggttcaa gcttgtactt
   113701 ttcgttactg cccgcatatg ttatatatat aacatcatac ttatcggtag tttcataata
   113761 tccgattact ttaaaacagg atattgttct gcaggaaaaa attcttcttc ccgattcttc
   113821 atattccgcc attgcttcat tcatacggac aataatgttc acattatcaa aacgattgcg
   113881 attagcttca tgcatcggta aaggtattac ggcatagcct tttacataat ggtctatatc
   113941 ggtctgtttc ggcactatca tgtccttact tgaatatccg aaatgcaatg tcactttaca
   114001 gtttttcttc atgcttttta tttcgcttat accggtaacg gcttgataga aaggggggaa
   114061 atcttcataa ttattccaaa actgactttg aaatccgaaa ttcatttgta tgctatataa
   114121 aggataatta taatatccgg gctctgaaga atctgtcgga tagtcgtccg gattaacacc
   114181 ctttacaccc gtacttatga caatatcgga tatatgctgt gaatatttgt cgtaatcttc
   114241 tatcaacgca tcgctatata catgtgacat ctcaagatca ctctttccgt caattcttga
   114301 tatgattatt tcatagtttg cgtaaataat gtcttgcaaa ggaacaacgg gttctatatt
   114361 caacgaatga acatacatga actcataggg ctcattataa acattaaatc tcccctcata
   114421 ttcggaatat acactccctt ccgcaaaata ttgacttaag taattaacca gtccggtaag
   114481 atccttattc tcatatacat ccaactccag gctgtcaaca tggttaggta tatctgtgcg
   114541 ccttgcacga attaattccg ttaatcccgc atatgagaca tataaagtgt catactcgct
   114601 ttttgtctta tactccccta ttatcgtgaa atagaatgta ttgccgaaat aggtatcact
   114661 tatgaacaat accttctcat tgtccggtat ttctttattg atatacttgc tcatggaaga
   114721 aggaataata gcacagtttt catagccctt taacaacaac tcttcggagt agccttcata
   114781 aagcattatc ttgcagtctt ccctgtcgga aagtattccg cttaaggcgg aaataccctt
   114841 gcaatacttt atcgcatcta taatttccct gtctttgtcg gtatcgtcct ttaccaaaaa
   114901 accttcattt gtataccatt taatattatt ttcttccatc ttatacctta cggaagatat
   114961 attgatgttt gctatttcct ctataaatgt ccttctttta tccagtatac tcttagcata
   115021 cttatatgac atattcagcg attcaccggt atccattctt tttataacaa tatcgctgtt
   115081 aaaagacgaa acaacattat tatcttcctt ttgaaacaga ctgtcgcgga atatatacaa
   115141 cattgcagcc gcaataactg caatgactat tacgataact ataacaacta ttgctttttt
   115201 ctttctttcc atactgttat tctccgctca gtctttcaat aatatattaa acgcacaaaa
   115261 tgtcctgtaa gttgtgttta tagtcgtata tattttacct taccgtatag caatattcat
   115321 aatccctgcc caatgcgttt ttctttcctg catattttga tgtatcggtt gccggtgcaa
   115381 aatactgctc cagatattcc agcaacggtg taatgtttgt atcttttttt gtttctatgg
   115441 ttacagagtt gatatagtca tttctgaaac gttcctttgt atacttatca ttgctgcccg
   115501 catatgttat atataccaca tcatatctgt catccgtctc gtaatagcct attaccttaa
   115561 aaccgactaa tactttctgg ttactctcca cagcatcatt tttctcgctc aggtataaaa
   115621 tatgggtttt taaatcggga agattgcttg cggcatcatg cattgtctta ggtattatcg
   115681 catatccttt tatatcaccg tcttttttaa aggtgctctc aatataatct tcctttcttt
   115741 gcacaacaag atccctgttt gtatagttcg tataaaaagt caccttgctg ttgctcttca
   115801 ttctttctat ttcacttatg ctggtaatag gctgatgcaa aagatattga acgtgttttt
   115861 tccaagcttt cccttttcct acatctgcta aggcatcttt acgattctgt gtaatcatat
   115921 ttgttaccgc atcgcgaaaa ccggttgagc ttgaagcgaa acttatattc agaccgtcag
   115981 cattatcaag aataaaattt gtctgtataa aaccaccgcc tttgtctatt gactcaagtt
   116041 ttgcgttttc ataaggatca tgtcctccca aaccggtact tatatttata tcggttatgt
   116101 attgtgagta tttatcgtaa tctctgacca atgcgtctgc atataaatag gacatatcca
   116161 aatcaccttt tccgtctatt cgggaaacgg ttattatttt cttttcacaa tctgtatcgg
   116221 gtgataaatc caacgacttc atgttgacgg agttttcata catgtatgag taatgctcgt
   116281 taaacatgtt tatcctgcct tcatattgag aaagtgtgtt tgcatcggca aaatatgtgg
   116341 taagacaata ggtaagtttt gcaagatcgg cttgctcatc tgcgtcaatc tccagacaat
   116401 caacaaagct tatgtcaatc tcttcaccta tattaatcaa gttaccaaat cccgcatatg
   116461 acagatatag ggtatcgggc tcccctttat ttttactcct gtcttttact tcatactccc
   116521 ctattatggt gcaataagct ataccgaaag cgcctttctc cagcactatt cttttgtcgg
   116581 aatcaggtat ctcttcgttt ataagctcac ctaaagaaga cggaataatt acataggttt
   116641 tatcaacact taaaagatgc tcctcggaat atcccgcata gaactccact ttactttttc
   116701 ctttgtcgcc gactatcgtg cagagagaag ttataccttt acagtacttt atcgagccca
   116761 taacttccgc aactttatcc tcttccgaag aaacaaacaa atccttttct ccgcgcatac
   116821 ttatatcatt atccttcata ttgtatctgc gagaagatat atttacagcc tgcactcctt
   116881 caatatatgc tatattgtcc gaaagcaggg cttttgtata cttatacggt atatttgccg
   116941 gttgcttacc gtcaaccctt tttataacta tgtcgctgct gaatgttgca tcaatcaaac
   117001 cttcttcttt ttttgacaga ccgtcacaag atgccaacag caatgcagtt ataataactg
   117061 ttaaggcgac agctataatc atatttgttt ttttcatgtt tttaccctcc aatcggttgt
   117121 ttgtaaatcc ttatgtaaaa cacaaattac ggcataattt ttactccgca atctccttca
   117181 ttgtaaaact gtattcatac gccaaaccca attcatttgt actgcctgca tattcagcaa
   117241 cattttccga aggtgcaaaa tataacttca aaaaattaat caacggactt atctccacat
   117301 ctccttttgt cttcatggtt atagactcta tatgttcgtt cttgaatgct ccacttttat
   117361 acttctcgtt gcatccgaca tatgttacaa acactgtatc atacggatcg gtagtctcgt
   117421 aataccctat tattttaaat tgggctctaa aatgaggaga tctgtctatc tgacttgggt
   117481 caactccgcc tataccgaca tataccctgc tctcatatac ttcaacaatg tgattgttaa
   117541 aattgggaag atcggatgtt gcctcatgca gcggttttgg aactatggca tagcctttta
   117601 ttttgcccaa caagtccttc tgtctgggta caataaggtc actgttcata tagttcagat
   117661 agtatgtaac tttgcagtct tttttcatcc ttaatatttc atttatactt gtaacggcct
   117721 gatgataaca gggaaagacc cccagcgttt ctttcagttt agcctcgtat actgtgtcat
   117781 attttaaaag caactgatat accggctgat tccagaaagc ttccgaaccg agaggataat
   117841 ctgccggatt aattcctttt actcccgtac ttatatctaa atcggttatg cattgtgaat
   117901 atttattata cccttttatc agcgcatcgg catacacatg cgacatttca agatcttcct
   117961 tcccgtccat tctggatatg gttattatgc tcttttcata gattacattc tctttaagtt
   118021 caatcggttc tatatccatt gaatgaacaa acatatattg ataagggtcg ttatatatat
   118081 tattcctttc cgtatactgg gaaagcacct gtgcatccgc ataatattca cttaagaaac
   118141 gcataagttt attaagatcc ttgtcctcat tcacatcaat ttccagacag tcaacatggt
   118201 ttagaatatc ggttctgccg gcacggatta aagtacttaa gccggtatac gtaacataca
   118261 aggtatcata ctcttcttca gtcttgtact ctcctattat cgtaaaatgc aacatgctgc
   118321 cgtatctcat attccttata ttcaacacct ttccatcggc aggcaaatcc ttattaaaat
   118381 acttactcat ggaagaaggt ataatggcaa agttttgata atcatgcatc agtaaatctt
   118441 cggaatagcc ttcataaatg gttatcttgc agtcttcctt atcggcaact atcgaactta
   118501 aagtggatat acccttgaaa tactttaccg cctctattac ctgcttacct ttacctctgt
   118561 cggtatctgt caaaacaccc tccggtgtat gccaatcgat atagttttca ctcatgttat
   118621 actttacgga tgctatattg atgccggcaa tttcctgtct gaatataaat aagttatcca
   118681 tcagtgcttt ggtatatctg taaggcatat ccaacgattc attcgcatcc gttcttttta
   118741 ttactatatc gctgttaaat gttgtaacaa caaaattgtc ttttttaaat aatctgtcac
   118801 ggaataaaaa caacagtgaa gccgcaataa ctataattac tgctgcgata atcaatatag
   118861 caaatctttt tttcctgttt tccattctgt cagacctccg atttaaatgc aataaaattg
   118921 catatatact gtattttcaa gacttaacta tccaaacaat actctgtttc tttttttctt
   118981 ctatatatac tttttattaa ttattttgtg ctacacacgg ttcattctaa cataaaacag
   119041 agaactgtta aataaattac ttaaattgtt cacaattgtt cacaattgga gatatatttg
   119101 ttaataaaac attgcatatt ccttctatat tatatccaac agccgtaatt gattggcata
   119161 ttgcggtatg atataatttt aaagaggtac ggaagtacca cggaaaggga tggaatatgc
   119221 ttaaaaaaag ctctgaattc aaagaaatac aaggaccgct tcttcttatc gtaatggacg
   119281 gagtggggtt atcggataaa aaagaaggaa atgccgtagc ggctgcaaat gctcctacat
   119341 tgaggatgtt gatggacaaa tatccgcata cgaccataaa ggctcacggt gttgcggtag
   119401 gattaccggg tgatgaggat atgggcaact cggaagtagg tcataacgca ttgggttccg
   119461 gacagattta cagtcaggga gcaaaactgg tttttgaagc catagaaaag aaaaccattt
   119521 tcagcgggaa aacctggctt aaacttaaag acaattgcat aaagcataat tctacaatac
   119581 attttatagg gttgttttcc gacggcaatg ttcattccaa tatagctcat cttaaagcaa
   119641 tgttggaaca ggcaaaagcc gaaggagtta aaaaagcgcg tgtgcatata ttattggacg
   119701 gaagagatgt cggagaaacg tcggccctgg aatatgtgga gccgtttgaa gagtatcttg
   119761 atactttgcg ctgtaatagt tttgacgttg aaattgcaag cggcggcggc agaatgaata
   119821 ttaccatgga cagatatgaa gccgactggg atatggtaag aaaaggctgg gaaattcatg
   119881 tattgggaaa aggacgcgca ttttcctccg ccaaggaagc tatagagaca tacagggcgg
   119941 aaaccgatgt ggtggatcag tttcttccgg gatttgtaat aacaaaagac aataaaccgg
   120001 tgggaacaat agaagataac gacagtgtta tattctacaa cttccgcgga gacagggcta
   120061 ttgaaataag cagagcattt gaagaaaaga actttgacaa attcgacaga gtaagagtac
   120121 cgaatgtaat ttatgcgggt atgctggaat atgacggaga ccttcatata cccaagaact
   120181 atttggtcag ccctccggat ataaaaaaca cattgggaga aaatctctcc aaaaaaggag
   120241 ttaaacagtt cgcaatatcc gaaacacaga aattcggaca tgtcacatat ttctggaacg
   120301 gaaacaattc cgccaaattt gagggtgaga catatgttga aataccttcg gataagatac
   120361 cgtttgagca acgtccctgg atgcaatgcg cccttataac cgataagctt ctggaagcgt
   120421 tgaaaagcgg agaatataag ttcctgagaa tgaatttccc caacggggat atggtaggtc
   120481 acacgggcag tttcgatgcg gctgtaatag gtgtggaatc ggtagacctt tgcctggcaa
   120541 gaatgttgcc ggttgtggac cgattgaacg gagtagccat aataacggca gaccacggaa
   120601 acgccgagga gatgtatgag acaaagaaag gcatcatatc aatagacagg aaaacgggaa
   120661 aaccggtgcc caaaaccagt catactctta acaaagttcc gttcattatc tatgacaaca
   120721 gaaaagacaa gggttataca ctgaaagaag gcgatttcgg attggccaat gtggcggcta
   120781 cggttgtcaa tcttttagga tatgtggccc ccgaaacctg ggaagaaagt atgattgagg
   120841 aggtttataa tggataattt ttacgaattg gcatcaaaaa gacagagttg cagaaactat
   120901 tcggacagac cggttgaaaa agaaaaactt atgcagtgca taaagtctgc ggccatagca
   120961 ccttcggcat gtaacggtca gccgtggcat tataccctta ttaccgagag tgaaacagta
   121021 gctaaactgg taagacattt acagtccgcc ggcgccaaca gatttacatc caaaacaccc
   121081 gcttttgcgg ttattactga aaaacctact aatgcaatgg catctatggg agagcgatta
   121141 aaggatcagg atttttcctc aatggatata gggatatccg tggcacattt tgtattgcag
   121201 gctgaagctt tgggattatc cacctgtata atcggttggt ttaatgaaaa cggcattaaa
   121261 gaactgctga atattaaaaa gtccgacagg ataaggctgg ttatagcggt aggctacaaa
   121321 gacgactcgg acaaactccg tccgaagaaa agaaaatcct tggaagatat tttaacggtt
   121381 atagaatagc ggcaaaattg ctgctttttt tatttcctcc ccttttttaa taattgcata
   121441 attatattgc tatttaagtc tttttgtgca ataattaaaa atacaggagg tatattcttt
   121501 tgaaatacta tgagaatatg gtagaaatgg gttgcttttc ccgtagtgaa cttgctaagg
   121561 ctttacaatt gggggataat acagcagcca ccatattaca acaatatcag aaaaaaggat
   121621 atatagaacg ggtcaggcat aacttttacg ttgtgataag catagagaat aagcaaccaa
   121681 tcttatcacg ttacggaata ggcagccgcc tatttcccga tgcgtccgtt tcacatcata
   121741 gtgcctttga agtatatggg tatgcaaatc aagtatttta tgaggtttat gttatgacaa
   121801 gcagtcgttt caaggacttt gaatacgatg gaataacttt ccgccgtgtt tcacctaaag
   121861 ggactgtgca aacggaaata ataggaggtg tccgtgttac aggagtagaa cagacggtta
   121921 tcgacagtat caatgatgta gataagattg cgggactgga ggagcttctc cgctgccttg
   121981 atctaatccc cactctgaat gaagaaaaac tgctatttac tttgaatgaa tatcaaaatg
   122041 gttttctcta ccaaaaaacc ggttttatcc tgaaacactt tcgcgagtct tacggtctat
   122101 cggatgcttt cttccggata tgtgcagatc gtatacctaa atccgaccgc tatctcacaa
   122161 aggaacatat aggatatgta tatcatccgg aatggagact tatggcacca aaggatataa
   122221 atacggtgat aaataaagga gtagattatc atgctgcact ttagtaaagc agaactgaac
   122281 agacaagcaa atgctcttgg ttttgtccgc gatacatttg aaaagatttg tcgtcttgca
   122341 gatgtgcttg gttttatgga aaataataaa aaaccacaaa aagtttttaa gataggaata
   122401 gcatttacaa tttaatcaaa taattgtcta catatttgtt accacgcaga actgttatta
   122461 aatgtttctt gcaatttgtt gacaaatttg ttacattttg caataataga gcaggaggta
   122521 ttttaatgac ggacaataat attaaagact ttcgtcagga tattatcaaa cagattacga
   122581 aagcacgggt tgctaaaggt ctcacacaaa gtcagttagc tgaactttta ggtatgcacc
   122641 gttccgccat atcacgcttg gaaagcggaa gtcacagtcc ttcattggac cttttactac
   122701 aagtggcttc ggttttggag cttaaaattg aactgacaac accttcaacg aaagtacttt
   122761 cccatatgga aaacaactac gagttacggc tctttgataa agtattacta actttctctc
   122821 ttgagaaaag aggaattgaa ggattgacag cacatattat ttctattgac aaggaacaac
   122881 ttcacttact cccgcttgga ttggaactct ctgataaagg aattttgaga tggttggaga
   122941 aacgagtgat tcctaaaaac cgtacctttg tgaaagagat cttgaaaact ttaaatctgt
   123001 ctcaaggtga tactaaaggt attattgata tttgcaaggg cttgtcgctt aacgacagtt
   123061 tttgggtagt cccggagggc tttgaaggtt cctttgctca gtataacctc tatgaaaacc
   123121 gtttttcgga aattctttct ttggttgcct atacgggtat agagcaaagt cacggaggtt
   123181 tcacaacctc gccggaactg actactgccg gaatgctgcc taaagcatgg cgccttattg
   123241 ataatgacgg tatttacttg tataaaggcg gcacattcgg tgccgctaat acgggaaacg
   123301 agccttacag tgaatattat gcttgccaaa ttgcacagac catgggtcta aatgccgtgc
   123361 agtatgaatt ggagaactgg aaaggtattc tagcttcaaa atgcaagttg ttttgtgata
   123421 ttgataccgc ttttgtaccg gtaggacgca ttgtaacatc gggcggttta aaagcggtat
   123481 taaaatacta ttcttcaatc ggggaagatt ttctccaagc ggtacacagc atgctggtat
   123541 ttgacgcggt aatctacaat gaggacagac acttcggaaa tttcggagtc ttaagagata
   123601 atcattcagg tgcattgatt gcccctgcac ctctgtttga ccacggactt tcactgttta
   123661 attatgctat gcctaaagac atcaaaaacc ttgaccaata tgccaaaacc cgtctacccg
   123721 cttatccgga tgtaacattc gaagacattt gtacagaagt tgcgggaaaa gcacaggcac
   123781 agcagttacg tcgtttgatc ggttttacct tcaagagaca tccgtccgtt aactggccgg
   123841 aagagcgatt gacggcaatt gaaaagcatc tgcaaaaacg agtacgtcaa ctgttgggaa
   123901 tggtaaaata aaaaggattc agtgttccaa caccttactt atcgctatgg acaactgatc
   123961 gggacaggat gtttctttat aaccgcattt aatgcctttc aacaggctta cggctttctt
   124021 tgcttccata ccttcaagta atttgcttat tccctgaaga ttcccgctgc atccgtccgt
   124081 aaacttcaca tctttcagtt ttccttcctg tatatcaaaa gttatctcgg ttgaacatgt
   124141 tccttttgtt ttatatctgt acatatctcc tcatatctct atttcttttg tcaatatttt
   124201 ctttacggtg tcgacatata tgatatgttc ctgttccaat acccttttcg ccaatgtctc
   124261 cacggtatcg tcttccagta caggaacttt tatctgattt agtatcttac cgttatcata
   124321 gtgttcatcc accacatgaa cggtacagcc cgattctttt tctttggctt ccagtaccgc
   124381 tttatgtact ctttctccgt acatgccccg gcctccgtat ttgggaagaa gggccggatg
   124441 gatattcagc accttatatc tctccacaat ctctttaggc aatttcttca tatagcctgc
   124501 aagcagtacc agatcaatct catactttgc caacaattcc agcaacttat caataaaacc
   124561 ttctttatat ttgtactcgc ttacatgata attatctatc ttttcctttt ttgcgcgctc
   124621 caaagcatag gcatcactgt tattggaaat aaccagtacg acttttgcat taatcttcct
   124681 gttcttacag gcatctatga ccgcctgcat gttggaacct ccgcccgagg cgaatacaca
   124741 aatattcatc atggaaatta tatcatcata tgctatggct gcacatacag aaaatcataa
   124801 atgttaccgg taatatcaat caggtattcc tttgtatgct ccttattctc gttttcggct
   124861 attattttta taaaatactt atcctttatt ctgttaccgg aagaatcaac ggtatcggga
   124921 cacagaggaa gtatatattc cgtattcaat gtgtagccgt tctcccataa tccttcataa
   124981 catatatcat tcggacacat gtcataactg cctaaaagat ctttataatc ataggtgttt
   125041 ccgtatctgt cccggtatgt gcggtttttc aattcttcac tgaacaggac cgacagtttt
   125101 gttactctgc ctgcaaccgc tacatttagg cgaactcttt ccagtgaggt aaatctttca
   125161 ttcagatgcc atatattcca gtaacccgtc agtgatatgt cggtgatgtg aacatcataa
   125221 ttcgcttgat acggaatatc ttccggcagg tcggatatta tttcctccgg ttgtactgga
   125281 tcagtcgggt tatacggagt ctcttcttta taaaggtcta cagaacaata ctctcttgtt
   125341 atatgaacat cgttaaatat gctcttaact tctattaata ccctcacctt ataccttgcc
   125401 ggcatgacac tctgaatcgg tatatccagc tccagttgcc cgtttctttc atgaggctgc
   125461 agaataacgc tgttacctcc ctgtaccgac agttttatat aatcaatttc cttaagaggt
   125521 ttgtctatgg tgaagtttac attgatatat cctttcttat gcacatcggc agcggaatag
   125581 gtaagccttt cttcagtaaa agcggcattc aggtttacat taggaacacc cattgaggga
   125641 accttgaaag tggcataata tccgttggtt ttgaaggtat agatggaacc ggtactgagc
   125701 caggatgcgc cgttctgcac ccttgtctta gtcataccta tatcatttgc ggtaaaattc
   125761 accgctccgt gccctataag atttgtgtgc agcatatacg gtttttgcac cgaaggctcc
   125821 agcctgtccc acgattcgac cgctccggat atttcggcat atatccactg attggcatta
   125881 acgggaccca tttcggtgtt ctgataagat atattggtaa aagggtcttc atcaaacgta
   125941 tatcccaaaa actgatattc acctgctaca caatgattat tcactacatc ttgaggatta
   126001 ccgtaaacaa tatatccgta atcacggtac attgtgaaag atgcggtata gttaccgttt
   126061 acacttttta caggtattcc taaatattgt atccatagta taaactcctc ctgatttgta
   126121 ggagtattgg aacacaaaga acagccgaac aaatcctcat atattgtttc ttcatatccg
   126181 catgttatgc atctgtatat gacggcatgt ccgaaatcgg tatgctcccc tatgttcgat
   126241 gtcttctgca tatcatgtcc gtgacataag caacacggtc tgtatgtagt aaacctgtct
   126301 tccgtttgtt tgcaaatttt acaggttctg ctctgtcgat gtccttgttc ctcatgtatt
   126361 tttgaaacag ccgaccattc ggaaaaatca tgatatcccc tgcacaggca gcagtcggca
   126421 agtgttgtat aagatacggt gacatgcccg catgcagaac aggtgccggt ctttttatgc
   126481 ccgtcagctg tgtgttcctg tgatacggta tatgagtaat tatgataacc tctgcaatag
   126541 cagcaggtgg gtactgtcgt gtaatagtcg gcattatgcc cgcagtcaaa acacctttgt
   126601 gtgacataat gtccgtatgt cgtatgtgca gaagatacgc tgtaactgta attatggtat
   126661 cctctgcatt cgcaacagtc ggatttctct gtatatcccg aatcgcaaac atagccgcat
   126721 ttactgcatt tcctggtata gtaatgtccc gatccggacg tatgataatc ggatatgctt
   126781 gttgtatagg aatgactgca attgcagata gcacatgacg ccagtgttgt atatccgctt
   126841 tctataacct ttccgcattc cttacaggga acggtgtaat aatggcccag tccctgcgta
   126901 tgctccctgc ttgtttccgt gagacctcga atatgaccga cacaatcaca gcagctgtca
   126961 tcttttttat agtaggaggt cgtgtatccg caggttgaac acttcaattt taccaaatgc
   127021 ccgtgagcgg tatgaaaccc gacagagagt gtatagtagt tatgattacc ggtgcaccga
   127081 caacatgttg aaagagttgt atatcccgtc acggtaatca aatcgcaatc ctgacaaata
   127141 tatccgcatt gatgaccttg cggagtgtgt acatcggaaa catatgcggc atttatgtgt
   127201 tttccggttc tctcaaaact ttcacaatat gcaaatgcgt taatgcacat gcttaacagt
   127261 aaaattatcg taatcaaaga caggatagct cttttcatgt tatcttctcc ataagttgcg
   127321 ctatttattt ttttcctata cttacctttt cattgttttt ttgaaaaagt aaaattgcct
   127381 ataacattat gcatagtgaa aaagacatat ctcaatgttt cattggagtt gtcttccaac
   127441 aggttatatg catcccaagg ctttaaagca cgtaaaaata catatctgtc tattacataa
   127501 tccgctatat catacggatt atgttcggat aatgctgcac acagtgcatt tttaactttg
   127561 cttttataga agctatcggg tataccgtca tcccagttaa acttcttcag ctccaacttt
   127621 aaaaacattg tatcgtaacc ccactgtgtt tcggtatatc ccgacgggcc ttcaaaaaac
   127681 ctgaaagcaa acaaacaata tgtggggttg gtatttatgg cggatttgtg gtatcgaatc
   127741 tcatatgtat tcttggtttc atatactctt ttttccacca ctatataccc gatatcatcc
   127801 ttgacctgtg tcagagtgga tttaataaca tcggataatt cctcatatgt aacacccttg
   127861 tatttaacat tatctttggt tgtattatcc agaaacgagc aatcgaattt caccgaggta
   127921 tctctctttg ttttatcgat aagtttgatt aataaggtgc aggcctgtgc tctggtaagc
   127981 gtttcattga atcgaaaagt gaaatcttca aacccgttaa taactcccgc actgtagcac
   128041 ttttttatat gctccttata cttatcaggt gtatatgcac ccaaatcaaa cggatatatc
   128101 ttttccgaat aaaattccgg ctccttaagg tatttcatgg cattggagat aagcaaggct
   128161 gcctgccctc tgttaatctc tttttgatat tcaccgtcaa atccttccaa aagcttaata
   128221 tcaccggctt tatctatgta attttgagcc cagtaacctt cagcattacc tatatcaaaa
   128281 cccagcgctt ttattatcat ttttatgaac tcatcggctt tgacattgcc gtcgggtttg
   128341 aatgtattat cgggatatcc gtttataata ttcattccgt acaactgtat gaccggttca
   128401 aaaaaccact gggatgattg cacatcgcta aaccgtgaac ctgtagcctg agcattaaca
   128461 ccgcatactc cggctgccgg tattaagcat atgataagca ataaagatgt aatttttttc
   128521 atgatactcc tttcgagtat catactattg ttattgccaa tatatgtcta ctgacaaaaa
   128581 tgcttttttt tatactataa gaaagaattc cgataatttt ttcggaatct cattccgtta
   128641 atcacgaaat attgtaaaaa gtcaatatgt aaaaaactac tctcttttac ttttctctat
   128701 cttttcttcg gtatctttca gtaccttttc tcttagggct tttaagaatt cacaaacctg
   128761 tgcttcttct ataccgtagg ttttctgcag ctcatactct atatccaacc aggtagatat
   128821 gtctgcctca ttatgctgta taaccgcttc cattttatcc aatgctttaa agactatcgc
   128881 ctcttttgtc ttcttttgct ccatctcgga aaagagttct ttgatatgct gatagcgttc
   128941 ttccggaagc tgccgtataa attccagcac ctgctccatt tcttttttat catctttttt
   129001 tgttttcctg aaagaaggta tatctcccgt tatcgcttcg cctatgtcat gaacaataca
   129061 catttccaca actctgtcca tgtttacatc gggtatagta tcttttaata catatgccat
   129121 aagtatgagc cggtaggagt gttccgcaac cgattcctct cttccgcagg atgtctttga
   129181 atgccgaaaa ttgcatttca tcttctctat aacatgcagc cattgtaaaa aatcatcagg
   129241 tttcattatt caccaaataa ccacgggtca tcagccggca cctctataat ttcttttaaa
   129301 gtcattatct gagagccgct tacaaatttc accaatcttc tgtctcggtc gtttgtatac
   129361 caaaaatagc tttctcttgc cgtgaataag gaaaatcccg catacttggc agagtacttg
   129421 acacactcat aagacttacc gttaacggta attgtttcca tcgtctgatt ggttactcca
   129481 taaggagcaa acattgcact ttcgtttaca agcacattta tgtacacctt atcgtcttgc
   129541 tctatctgaa ttaacccgag tgtcaggtta atcatttcat tgtcaagata tcgtgcaggc
   129601 tttgctactt ccttatccgc ttgcttgtct tcctgaatcg cttttatagt tgccttttct
   129661 ttcgtatagt cggtttttac cgaccagtct ttttcttttc cggaatagaa aacattggat
   129721 ttgtacgaag aagacggaat aagagtatag gtgtcgaata tgacttgtgc tctgtattgc
   129781 cacatatcaa actccgcata tgactgcatt tttgttatgt tcccgtctgt tacatacttg
   129841 aatgtgaggt ctccgtcaat attctgctct tcgtttacta tgtagtatac caggcggtag
   129901 gaccctttcg gtatttcacc gaccgtatat tcatcttcgc taagctgtat tttgcatccg
   129961 gttaaggttg tacaaaacaa cgatatcaat aaaacaagag ttaatgtttt aaatacttta
   130021 ttcttcatct taatccttcc tcttcatctc tatttcaaat cggtcaaagt atactttctc
   130081 cataaactgc tcatcggtga aaaatgtctt gttgaatcgt gcaagctctt tggtgttttt
   130141 actcatccat accggaacct gtatatccat cttcttgcat atgtccacaa gacagtttgt
   130201 gagtctttgc tgaaagttca tgtcgggatt gacacattcg gctttttctt caaccagtat
   130261 taaattgttc ttgataagtt tgccgtatat tagcattatt ttccgtcctg ctcattttgt
   130321 attgcctatt ttatcactga atgagtgttt tttaaacatt ttatcttatc gcctattgct
   130381 atattgtgca tgtaaagcaa gttcctgccc tgacctacat cgcttccacc tgcagtattt
   130441 tttccgcaag agttttactg aacttctccg gttcttccgc taacggactg tggcttgatc
   130501 ggttaaacca tatatagtct ttaagcggag cctctacggt tttatagtat tcatacgaca
   130561 gttcaaacgg tgtattgtaa tcggaagctc ctatcagata aaacatggga acggaaaact
   130621 ttgtgtactc ttgggaaaag tccagcttaa ctatatcttc ccacatgttt ttcagagaaa
   130681 aactgtagcc ttttaaaacg ccggctatgt cgcacaatgt gtactccttt gagaaaagta
   130741 tcggtttaac ggtactcttc cacattgatg agcatttgaa tgttgcaccg ccgtattttg
   130801 ataaaagttt tctctgttcc atcaatccct ttatcggatc gcctgcataa agcccctcta
   130861 ccggtctttt tatttccaga agcttctgca catctttctc attaccggca gcctttgctt
   130921 tttccagcac gtagtcataa cttttctctt catttcttat cccgttcaca agttgcccct
   130981 gtccgaaata cgcttttata ttctcaggat atctgtatac cagcattgtg cctacgcagg
   131041 atccccatga atgtccctgt atgtatatct tgtctttatt gaatttttta caaagatatt
   131101 ccgtaatctc ccttgcgtct tccacaaagg tatccaaatt catatcctct ttctttgccc
   131161 ctttatagct tccgccggta cctctttggt cccatcccgc tattgtcact ttgtcagtta
   131221 tatctaacat atacttcatt acgttatgtc tgtccactac accgggtcct ccgtggataa
   131281 agagtacaac gggattaacg ctgtctgttc ctataatatg aatcttctgt tgtatcccgt
   131341 taatcattac cttttccgtt atatcaatcg tatttttcat atttaccccc ttttttgtag
   131401 cctcttccgt tggatataat ttcggaagtc aaaagtgctc tgccatatat tcttaacttc
   131461 aaaacattct tacacacacg tgcttttgtt tttccgttca tatccttgaa gtatgtgctg
   131521 aaccgaccga aaccgaatat tctgtcctga cccccgtcat tgtacattcc gttgatgaca
   131581 taggaaacta tatcgggacc tccgtcaatt attatggcta tatggtttat ctgatcgggt
   131641 ttcagcatat atttatcgga actccggcaa caggtattta taccgtcatt cattaatata
   131701 ttaattctgt agtcggatat ctgttctatc agcactcctt ttcccgaagc atccctgctg
   131761 tctaatatct ttctctttcc cgttcccgct ttgtatataa actcgaaggt aactccgctt
   131821 tcggtactct ttgttgcaaa tatgtgactc ttcctgtcgg tgatacagaa tacgggaagt
   131881 tccggcatat caattaccga tttgccttcg ctttctgaaa gtaacccctc atcggttata
   131941 tccgatgact caaactgatt ccaaaggctt tgcaggaatt tttccggtat ttcatgcact
   132001 cttgcatcga gtttttgtgt ttcggtaatg tagtatttgc cgccatcctc tatgaaatcc
   132061 ggataactca ttctgacacc gggatcttca tgatataaaa caacttccgg ctgcgaccac
   132121 tttatcctcc tgccttcttt tgtatcatat tcgataccgg aacataaaaa tgccggattt
   132181 ctgttttcat accatttgct tccgttgttg tggtaccagt acaggtattt gccgttttta
   132241 cacttccata cgaagtttgc cgctctcgga tgtttcatca atctattgtc ggcatacctt
   132301 ttgtattgcg gttgctccca tgtatgtcct ttgtctcgac tgtatgtttc caccggatga
   132361 ccgtctatag aacggtatac acagtaaata cttttatcgc ttaatacaac catggattgt
   132421 tcttcggcaa tcaatccgcc gcccttaggg gtaataagtc ctatatcgcc gtcgggcagt
   132481 gtttcccaca caagcttatt gggatctttc tcaatgttca tattaaccgc tcttaacagc
   132541 cagccttcgg atacggtaag catatcgcct atcttgtgga ttataacgta gccgtcattg
   132601 tccaatataa aaggtttgcc cacggtccag aacatgcgaa cttttcctcc gtacggattt
   132661 tctctgtcta tctttgtttc tcttataggt acttcgtatc tttccgacca ggtctttccc
   132721 gagtcatcgg agtatctgaa tacgaaagct ccctgcatat ccactctgtg agacagacgc
   132781 tcttttctgc atatcagatt tcttaagttg tttttattga aattatagaa acagtaaatc
   132841 ctgttaaacg atgttttcag caacactccg taagccgact ctcttaaatc atcgccttcc
   132901 ggaaagtaca gatcggtcca ggtattgccc cggtcataac ttacggttat accaacatgt
   132961 gttccggccg ctccttcctg tccgtatgcg gttgtcagaa cgcagaccca tgctccgtca
   133021 tccgcaacca cgacatacgg ctggtcggta tactgctccc taggtatagg ttttcctctg
   133081 tcaatgtatc tgaaatcttc cattttgcca cctctttaaa acacggtgca tatacactgc
   133141 ttatataact gcataaataa ctactcgtac tgttttaccg gtatggggtc accgagatat
   133201 ttgggctgtc ttttcataat atccgtttcc ataacggcct ttatcctggc taaactctcc
   133261 cttatatgat tatacgtcat attgtacatg ctcttgttct ctgcggcata tccgtatgct
   133321 ttaaagccca acctttttgc tatatacaat gctctgtaaa tatggaatct ctgtgtgcat
   133381 attattacgc tttgcacttt gaatatatag cccgctctgt atatgctgtc ataagtgttg
   133441 aatcccgcat aatctctgta gatatcgtct gcattcacac ctttggatat aaggtaatca
   133501 tacatgacct ttgtctcgtt gtactcaacg gtgccgttgt cccccgacac tataacagca
   133561 atctttccgt catagtattc atatacctga tatgcgtaat ccagcctgtc tttaagtacg
   133621 ggtgaagggt ttccgcttgc gtatacagcc gcacccaaca caagaacggc ctgaggtctt
   133681 tcaatctttt ccaactgctc aacactgtca aatatatcgg acaaaggata aagcattaca
   133741 taaagctgta atgacgtaaa cagcagtgcc gatatcagta aaataatcgc tattgccttt
   133801 tgtgctctgt ttttcttttt cttttttctt ttagaacgca ttatccctta ctgcctgctc
   133861 cagttttttc ttgtcttccg ccgtaagatt cttaaaaggt tttctcgcct ctccgcaatc
   133921 aattcccgca agttttgaga catatttcat agcctgaata gtagcaccgc tttcaatcat
   133981 gggttgtata atgttgttca gttttgtctg taatttttgt gccgaagcaa tgtctttttt
   134041 gtcaaaggcg gcttttatct gtacaaaagc ctgcggatat atgttcattg ttgttccgat
   134101 agcaccggtt gctcccgcca ttaaagcgga agcgaaagct tcatccttgc cgctgtatat
   134161 cggttttttt gatgcaaccg ctattctgtt taacacatag taatcggcag cggtatattt
   134221 aatggagtaa atcctgtcat cggacaacaa atcgtttatc tggtctaccg acatatccac
   134281 acccgtagca ccgggaatgt tatatatcat tatctttaag ccgcatgcat ccgctatatc
   134341 ggtatagtac ttttttatag ccttatgtcc gtgtgagtaa tagaacggag gaaccgaagc
   134401 taccgcatca acgcccatgc tttttgcatg tttcgcatac attacggcta acgccgtacc
   134461 gggtgtgccc acatgtgcca caacagtcgc tccgtttgct tcttttgcgg ctacttccag
   134521 cgttttcatt ctttcttctt cggttaaaag aaaggtctcc gcggaactgc cggttgtata
   134581 tagtccgtcg gctcctttat ccaacacata tcttacaagt tttttcagcg cctcataatc
   134641 cacggtctga tcttttttaa acggtgtgaa tatcggaact aaaatatcac atttcttcat
   134701 gagcaaatac ctcctgctat gttaagttta atggatactc gccttattta caatcaatga
   134761 tgttatgttt tatgataata tatattcaaa tatattttgt taataagaaa gaagagaaaa
   134821 tgaaagatcc gagaaacgaa aaactggcag atttattaat caactattcc tgcagactta
   134881 aagaaggaga gaagatactc atttccgtct ccgagaccga taacgacctg gttaagaggc
   134941 tggtggaaaa ggcatatcag gcaaaagctc tgccttttgt acagatatcc gactcgtccc
   135001 tgtccccgaa actgtatgag catataacgg aagagcagct ggaaataatg aacgagcggg
   135061 acctgaactt catgaagaag atggatgcat atatagccat acgggcaagt aagaatatat
   135121 atgagataag taccgttccc tcggaaaaac tgtccctggc atctaaaata ttgaaacctt
   135181 cctttgaccg aagagtcaaa tatacaaagt ggtgtgtgct gcggtatccc aatgagtcca
   135241 tggctcagct tgccggaatg aataccgaaa agtttaccga tttctattac gatgtatgta
   135301 cgctggatta tcaaaaaatg agtgacgcaa tggataatct ggtggaactt atgaataaaa
   135361 ccgataaagt gcatataaag ggagaaggta ccgatttgac tttttccata aaggacattc
   135421 ccgctataaa atgtgccggt gaaatgaata tccccgacgg agaagtattt accgctccgg
   135481 taaaagactc ggtaaacggt tatgtaacct ttaacactcc ttcagtcagt gaaggtaaga
   135541 catttgaaaa tatccgtctg gaatttgaaa aaggtaaaat tgtaaaagct acagccaact
   135601 ataccgagca tttaaacaag attttagata ccgacgaagg cgcaaggtat gtaggtgagt
   135661 ttgcaatagg tgtgaatccg tatatattgg atcccatgaa agacactttg tttgatgaaa
   135721 agatatgcgg atctttccat tttacccccg gtgcctgcta tgacgaagca cccaacggca
   135781 atgattccgc cattcattgg gatttggtgt gcatacagac cgaacagtac ggcggaggag
   135841 aaatcaagtt tgatgacagg ttaatacgca aaaacggtat attcacggtg gaagaacttc
   135901 actgtcttaa ccccgaaaac ttaaagtgag gactttatga agataatgca tcttgccggc
   135961 ggcggtgata taggaggagc aaaaacccat ataatctctt tggcaaaaga gctttcccgt
   136021 accaatactc ttaagctggt atctttccga tacggagatt ttgccgaaag cgccaaagcc
   136081 gaaggcattg atgtgacgga aattaaaagt gctttccttc tcaaagatat tcatgccctt
   136141 tttgttgaat atgacaaatt taaaccggac atagttcact gtcacggagc caaagccaac
   136201 atgatgggac tttttcttaa aaggcacaga aatgcgtgtg ttaccaccac tatacactcc
   136261 gattacagaa aagactatat gcataactta ttcaaacagg ccactatagg tctgttcaat
   136321 acattggctt tgagaataat aaagtttcac attgccgtta ccgaacagtt tgcaagaacc
   136381 cttatatcca gaggctttga ggcatataac atgttttcca tatacaacgg cattgatttt
   136441 gaaaaagaaa tgcctaagat atccaggaag gaatattttg aacaatatgg cataccgtat
   136501 aatcccgacg agttgattat agggatagcg gcacgtctta ccaaagtaaa ggatataccc
   136561 acccttataa aaggctttgc caaagctcat gccgataatt ccgatatgcg tcttcttatc
   136621 ggcggagacg gcgaagaaaa gcaaaaactt acggatttgg caaatcaact gggtgtaagt
   136681 gaaaaggtgc actttttggg ctggatagac gatgtggaca gctttttcag tgtaatagac
   136741 attgatgtaa tatcctcact gagcgaaact ttcccttact ctttaaccga aggaatccgg
   136801 gcgggagcca tccccgtttc cagtaatgtg ggaggagcat cttcactgat tgaccagaaa
   136861 gttaccggat acattttcga gcccaaagat tatgagactt taggcaatta tctgtatcaa
   136921 ctgtcaaaag acaagaataa gcgcataacc atgcaaaaag cttcctatga gcatacaaaa
   136981 gccaacttct cgctaaaggc aatgaagaat aaacaggaag atatttattc gcttatactc
   137041 aaacacttta acgaaaagga gcgcaacaag gtagttatat gcggagcata cgggcgaggt
   137101 aatgccggtg atgaggcaat aaaatacaca accttatcca gtatgaggaa tatcgatccg
   137161 gatatggata tatgtgtaat gaccttaagg cctaaaaaag aaagagcaat cgacagagtt
   137221 cagaccatat acacctttaa tgttataaag ctgtttttcc tgttaagaaa atccaagata
   137281 tttgtaagcg gaggaggttc ccttattcag gatgtgacat catcaagatc gcttttcttc
   137341 tacaccttta ctctttatct tgccaagaaa ctgggctgta aagttataat gttcggctgc
   137401 ggaatagggc ctgtatccag accgttcaac agaaaactgt cgggaaagat aattgataac
   137461 tatgtggatg taataacggt acgcgatcat atgtcggaaa aagagctgca atctttagga
   137521 gtaacaaaac ctcaaatcac cgtttccgca gaccccgcaa taaacctgac cgccgataaa
   137581 agcaacaccc ataagatatt catttcggaa gatatacccg cagacggaaa atatatctgt
   137641 ctggcactta gaagctggaa aaacttcaac aggaatgaag ccgttgcaaa agcctgcgag
   137701 catatgtata acaaatacgg atttaccaca atattcttcc ctatggaata tcccaaggat
   137761 ttatcggtgt cggaaaagac ggcggaactg ttagatacac cttactattt aattcgcaat
   137821 aaatatccca ccgagcaggt agtggcattc atgtcaaaga tggaacaggt tgtggcaatg
   137881 agacttcacg cattgatatt cgcagccgca aattatgttc ccgtagtggg aatatcctat
   137941 gacgtaaaag tatcgggctt catgaactat atagaaagct cggcatgctt ggaactttcc
   138001 gatgtaacgg aacaatccgt tattaatgca atggaagaga gttttaaaca tagggagaga
   138061 gttataaaag caacaaatac gttaaaggaa aaagaagcgg tcaacaagca gcagtttatc
   138121 aagttgttgg agaattaata cggtcttttt cagttgactt tatcctcttg tttcgttaca
   138181 agtttaccgt ctctcatgct taacacctcg tcggcatcgg cagatatctc cgaatcgtga
   138241 gtaacaacaa taacacaata tccttcttca tgggcaagct ttttcagtat gcctattatg
   138301 ttctttccgt tctccgaatc taaattgccc gtcggttcat cggcaagtat tacatcggct
   138361 tttgctgcca atgcgcgggc tattgccact ctttgctgct cacctcccga aagcattgac
   138421 ggcaatcttt tatggaaggc ggagtccaat cccaccgagt ccatcatctt agcagctgta
   138481 acctttgctt ccttatcttt gattcctttc agatgcagag gatacataac attctcaata
   138541 gcggtaagaa gagggaacag attgtaatcc tggtatatta ccgcaacatc atctcttctg
   138601 tacttgtcca gattcatctc tttagtatct ttccctttat acacaaccga cccttctttc
   138661 ggaacatcca gccctgcaag cattgctaaa agagttgtct taccgcatcc ggattttccc
   138721 acaaccgcat ataacttacc tctctcaaac tcaaaactga aatcgttaac ggcttttact
   138781 gtctgatatt tattcctata ctcatatacc gcatttttaa cttccaaaat cttttccatc
   138841 tcttctcctt tactcatttg ccttcattat tttcat
//
LOCUS       DAOSOM010000003       120229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_130_length_120229_cov_8.384676, whole genome shotgun sequence.
ACCESSION   DAOSOM010000003 DAOSOM010000000
VERSION     DAOSOM010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 120229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 120229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..120229
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130_length_120229_cov_8.384676"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..893)
                     /locus_tag="PLF14_01320"
     CDS             complement(66..893)
                     /locus_tag="PLF14_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT69974.1"
                     /translation="MEKEKTKVKARIAVFIIVAILIIVAAVFLFLLPGNKEEAPSLHE
                     IPVRRIGQDAEIDLNMPYEYVRSMINRQEDFLANAEDIEAQTVLSKQRDDISLAYKKS
                     WHIGPIDRLSYYQGITSIKTIERKDNCKVTFIKGYDSSYMMKKNEEEKKGYVILPAWM
                     KENIAKGYTVDDIDIMLINLEPYHLSRNFKVIGFYQADTQDTIYVSMYTYGELFEYDE
                     AKLNKMIKSLSIYYEEEADVSAIYGLMNEYFSDSATKKEGRNCVYTDYDFYYIPNVK"
     gene            complement(1097..2398)
                     /locus_tag="PLF14_01325"
     CDS             complement(1097..2398)
                     /locus_tag="PLF14_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOT69975.1"
                     /translation="MKRKAYKLLILIIVAALLLVLCGCAEPVISPSGTTVVLPSPTPT
                     PTPTVIPETETPSATPEPTPEPTEEIKPDAEDITKKVYVSVDGHYSDKLSDYSFYSKH
                     KLNKGSKIEISSYEKMHSLYIIWDKIPGEWTLNIGDEKLTCGKNGFIHEYIELPCGCT
                     RISIELTNDGATICDVYVFTYGNLPGWVQIWNPPYEDADMLLLPTHADDEHLYFGGMM
                     PYYAGQLQYKVQIAYLTNHWGEPYRPHELLNGLWTVGMTAYPVISEFEDKYCSNLEHA
                     KSVYPLEDVLNFQVELLRRFKPEVVIGHDLKGEYGHGAHMLNAYALTLAVEYAADSTK
                     YMSSYEKYGVWDTPKLYLHLYEENKILMNWDIPLERFNGLTAFEMAVQGYDCHKSQHI
                     WSFAVRQGESQYDCRWFGLYRSLVGPDVEKNDVFENILSGK"
     gene            2530..3573
                     /locus_tag="PLF14_01330"
     CDS             2530..3573
                     /locus_tag="PLF14_01330"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOT69976.1"
                     /translation="MTNKLGAAKRAVSVILKTLAVFFTTLALIVVALLGVIYVLVKGP
                     SPTAKKLFVLSVRETSAVGFLADIYLSDEELATLTVSEIVEEPKEKTDTSLIEIPTPT
                     SSLPPSGEDADITDTVPEEKDIEVVRIKGSLYEAVMLIVKDPLRVFVGTPDELGGKGL
                     KLTEMVEKYDAVAGINAGGFYDPLGGGHEGTPDGMVICNGEVVWGDPDVKLNVSAFDD
                     KGILHVGMMTTQEAMDAKIQWAVSFGPPLIINGKVQSFEKDLISGLNPRTAIGQRADG
                     AVLMLVVDGRRMNSLGATYADLADIMLDFGAVNASNLDGGSSTLMIYEGEVLNVCASV
                     TGPRALPTSFLVR"
     gene            3576..4988
                     /locus_tag="PLF14_01335"
     CDS             3576..4988
                     /locus_tag="PLF14_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT69977.1"
                     /translation="MRDKAVMHNKDNAGKPKSKFVKGLIIYSICFLVAVFIGLVFFWQ
                     YIKSYEISRPENYMNEFISTVNDDTVRTLMTENKKIEVSEFEEENTVINQLYFSLLKN
                     EEYKYRKMPGQYADTSPVYIIYTDSADLGKVVLTSKGDNSAKYGFNLWQTGSFTVLDW
                     VLLPEQKTLSITVPEYADVYVNNIKVGESYITDNAVAYTGFEYVNQPFDKRPTGKKYE
                     IPGIYLEAEVTAEDDKGLPLELNKTENEYAYPLVIKETYSYKVTAPKEVKVYINGVLL
                     TEKYIENGEAYYPGQENITTYSQVPLLNTYSADNLYYKPDIECYDVNGKSLEVTKASD
                     SKNIFYHYPADEQLKNTHEQHALNFAKTYINFTVNATGDIWGYFPTLNAYLVEGSVLQ
                     SRLKSACASLYWVMGSTLEYKYLKVIDFRPVSETCFTCTMSFSAIHKTYYETRYLEDS
                     FDLVFILYNNKWKVAEMLSE"
     gene            5004..6083
                     /locus_tag="PLF14_01340"
     CDS             5004..6083
                     /locus_tag="PLF14_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyltransferase family 2/GtrA
                     family protein"
                     /protein_id="HOT69978.1"
                     /translation="MTFKDNGLGITAVIPSLNPSQKLLTTVSGLIEQGFSDIIVVNDG
                     SSPEYDGIFDEVSSTEQCTLLKHEVNKGKGAALKTAFSYIQQRNKDIKGVVTADADGQ
                     HLPEDILKCSQHMTDDKTVILGARNFHQPDVPPKSFLGNKITSFVFKTGCGININDTQ
                     TGLRVFPVKLIPFLLSVEGDRFEYETNMLLEMKPEGIPFKEVEITTVYEDKNVGSHFK
                     AVRDAFRIYKLIFKFMLSSIISFLLDNIVFYVSLLIFANYVENTRILISTVIARALSS
                     FFNFNFNKSSVFKHKCDYKKCLIKYYVLSVSVMLASAGLVTLLSLIIPAEKEIIITLI
                     KIGVDAILFFVSFHVQREWVFKKCK"
     gene            complement(6401..7198)
                     /locus_tag="PLF14_01345"
     CDS             complement(6401..7198)
                     /locus_tag="PLF14_01345"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT69979.1"
                     /translation="MECNLFYTELSRGIYRISDSLHGPNSDGFSDAPGRATQNSYLIQ
                     GQEKAALIDLAVDTPDLFSYACQLTGLPTQVLLTHGHPDHVYNLESVGEAWLHPDDYD
                     LVLKGIPGICFPIHNVVLNPLKDRQKIDLGERILEVIHMPGHTLGSVLFWDKQSGFLF
                     SGDSCSRRLLYGLTPTVPLDEHCKKLAGLAEFPINHIYTAHDRCGLPKAHLLRICELI
                     RQELPKSATTVIIPGIGIMRNLRWGDENTLDYFDMAVEESYVEGLLK"
     gene            complement(7227..8357)
                     /locus_tag="PLF14_01350"
     CDS             complement(7227..8357)
                     /locus_tag="PLF14_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014369445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOT69980.1"
                     /translation="MKNHSIRPGKPFLDTEGKRIQAHGGSIFFDAETETYYLYGENKE
                     FSVPGSGIWTWGVRAYASRDFYNWEDKGLIVEPVLDDPLSPFNPRTACLDRPHILFNQ
                     KTEKYVCWMKIMHRDDNRRQTVTIAVADSFLGPYSIVQSGVSPLGMNAGDFDLVDDEN
                     GKAYYYFERVHSELICAELTADYTDVTDVCSHHFPHPYPPFVREAPAHFKRNGLHYLI
                     TSGTTGYFPNRSEVAVSESWHGPFTVLGDPHPGDPTGTSFHSQISSVFKMPGKKNLYV
                     ALADRWMPNAMEIPAAFCEEIFEGFFSGRVPLSEGEHFRESLIRKYGLQGYRSILVQN
                     DTDTSVADYVWLPLRFEEPAPQHPNGMIFIDWLDEWRIEDYS"
     gene            complement(8359..11040)
                     /locus_tag="PLF14_01355"
     CDS             complement(8359..11040)
                     /locus_tag="PLF14_01355"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOT69981.1"
                     /translation="MAEAAKQKPKDGLKEQLHFGRNRDMKLYHLRVNHRTNPCVDGDI
                     EFSWRISSEHKNIVQRSYRILVEGEKGTMWDSGTVESRRQSFVAYDGPKLPSKSEYRY
                     TVTVCTADGDSSGASGRFYTGLDLADWKACWVESTFMRNPHQMLSDGIENPVLSFTRG
                     FEIRGKLRSAVMYATCYGVYHLIINGERADDREFAPEFTAYSKLLYYQRYDVSGLLHT
                     GKNKLEMLVGDGWYFNSQTVPVGCDHEKPAILYQLELEYADGTRETVFSDGSEECRQT
                     NILYSDLFVGEKIDLTKPYSEPLRPVLCDYPFDILRAQALDPVRAVEIFSVKKVIQSP
                     KGELIYDFGQVIAGRCRVLLREERGTEVRIHHTEVLDKDGNYFAALSSRQRETVICSG
                     EKTLYEPIFTFHGFRYIMIEGARNIRPEDVTAVLLSTEKENKGGFNCSNPKLNRLYQN
                     IRYSQRNNMMSIPTDCPQREKAGWTGDVLVYNAASMLNEEMTPFYESWLSSLLADQQE
                     SGAVPITCPSTKMYDFMLRKISREYDKSKPAKGLEDILPAEGESAAGGELPSVAGWSD
                     VIIELPYSMYRLTGNDLILKKCLPAMKKYANQLIRTAAMRRGGASLDENDRYLWNTGF
                     HFGEWLVPGKEKPGFEACKETAWYIAPFYAFRSVNLLAEICEKLGDEKSAAYYGSIAR
                     KMKAAIEAEVLPLQHEYADYMGRYILALAFELVDGDLEKEYAGKLIELIHSNDYRIGT
                     GFLATPYILKVLDSIGRHDLALKVLMNPNCPGWLYEVSMGATTIWESWSAVAPDGTPQ
                     KISFDHYAFGIVDEYFFSVLAGVRPLEPGFARFEVSPDRVIGLDRLKREFICEAGLIS
                     VEYTDSVLTVTVPPNTRAVIKWNGETKEVGSGTWRFE"
     gene            complement(10937..11986)
                     /locus_tag="PLF14_01360"
     CDS             complement(10937..11986)
                     /locus_tag="PLF14_01360"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT69982.1"
                     /translation="MITPETTIKEIRSILQDNLFSQLIYDLSERGAGRFSDESRTLKD
                     IQAADPAWNADDMVFGLNTLCRNAARGRVLYDVYRSDETAACPGKANVKLVHFPAETA
                     CNYEKDCVRRTLILASGGAYGAVCNLPEAFPAAAEAAALGIDCLCLTYRVGALAPLFP
                     KPMEDLAAAIRFLFSNEQNLDVSMKHYAVGGFSAGGHLAACGGIKELGYRKFNLPAPE
                     AVLLAYPLLNVWKALEQLPEPYRKLMLKGLLGSGVDESYCGPYNVDQNIDCDYPPCFI
                     VHARDDETVDCELSSAFARALTTAEVPCVIEKPEHGGHGFGRGGKTEAEGWIKRAITF
                     WKEQRHETVSFTRKS"
     gene            complement(11992..13185)
                     /locus_tag="PLF14_01365"
     CDS             complement(11992..13185)
                     /locus_tag="PLF14_01365"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOT69983.1"
                     /translation="MKTETINQVMQGYIDSHELAGGALHVFSEAETIFRGKWGLSDIE
                     ATRSVEYDSIFRLMSMTKPIVAAAVMICAERGLIDIDDPVSHYLTEFADMQVNHDPKY
                     ANGPDLLKRIPLLLPFFNISKVKTKPASRDITVRDLLSHSSGMQQGTVSLLSLLKGLP
                     QFGSLREEVSYFAAQPLDFDPGTGTGYSPLAGFDVLGYLIECVSGTRLEGFVRTEICD
                     PLEMFDTGFFLSEERQKRLVKVYKRHAGSLKDVTGTKNDVAGAMHQKAISFEHGCGGM
                     YSTLDDYSHFAQMLLCEGSYKGRRILQPETVRMMRSEAPLVHLEPEPGFVWGLGMKIR
                     QDPEKGGSPATAGTYGWSGAFGTHFFISPIDRLGVVFMTNRADLEGSGSYISKRIEEL
                     VFEGD"
     gene            complement(13175..14638)
                     /locus_tag="PLF14_01370"
     CDS             complement(13175..14638)
                     /locus_tag="PLF14_01370"
                     /inference="COORDINATES: protein motif:HMM:NF019157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HOT69984.1"
                     /translation="MFRNICVSFNGSGEYHPKVSLIQRMFGGGKKIEGLPAYYEVELE
                     HLTGNHTETLIVWSPAAWNDRFAGTAGGGTGIGGRSYLTKPDNAQRGLTVPFAVMNGF
                     TAATMFAGNNDGGLHDYTIDPKTGTLNRELYENWRLRSTHNMTVFGKAIAELLHDRPV
                     RWSYMNGGSGGGRQSLMEVQNYPKDYNGIWASCPAINWQKLMVSGFWAQTLMNEYGIT
                     FSAKKNQFFIDAVHEANGGADKYYHLEKIPEFDFYSCVGKNIGSDSITEADARFMTEL
                     CRGPRKADGSFLWYGFAPGVKNWQCVIPIGTYYYPMPWSRHVKPFFLAPICARWITET
                     PGQKFDGMKIREFTELFEEGVRKFSDNLGDNPAIDAFVNAGGKLIIDHGMDDPLIPVA
                     GTLDYYGKLLTHFGSQSALDRFMRLYITPGDNHGNCFGNGPGITEKDGMLALMKWVEE
                     GKAPEEIRKVRVDRKNGETLEESSQSPYRDERHNNED"
     gene            complement(14780..16261)
                     /locus_tag="PLF14_01375"
     CDS             complement(14780..16261)
                     /locus_tag="PLF14_01375"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT69985.1"
                     /translation="MKSKESKPKIDKSLWLTTKSERINYYVGDLGRTLEGYIVTAFMT
                     LFLLFQGINLISISAAILVVKVIDALDDVIFGFLIDKLNIKKSRLFGKIAGEGKYLPW
                     YRATFFLFPIFTVLFFLMPQGVPNAVKIGWFMVTYLLYDISYTLVEVPMNSMIVSLTD
                     NTEERNSILQTKGIIGGIATVLAGIIWTFLISEHVGISIRMVAIVSSVIFFFMMLPLA
                     TKVKEHNTELKNTEDEKSTERYNFRDMIKCVKTNKYMAVILLSTLIMGALATGSSLGT
                     YASYYLYGDSMILIIPIAIAFIPQLIAQLNTDKLCRKFGKKKVYMVCGLTAALIYGSI
                     FFAGYNNFVVITILLVLQAAPGNVANIAKTFFTPDTIEYTRYKTGKDCSGIFFSLNAF
                     VNKLTTSIASSLGVFILGLSEWITVEADSFEELAELNVVQTQSALDTLWIVFMLIPAL
                     GTLLSVVVMAFYKLKDDDAKLMAQCNAGEISREECETRLSRKY"
     gene            16413..16934
                     /locus_tag="PLF14_01380"
     CDS             16413..16934
                     /locus_tag="PLF14_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT69986.1"
                     /translation="MKAVTSHEFVLESLTSALIGLMKTKSFDKITVTELCEKACVGRV
                     SFYRNYNSKQDILVKYLAACTNEWWQEFSQKPEKEFYENFWPSLLDEFKKQKELLLLL
                     NQNNLNYLLRDHIFYCCGPSPDEDDNVAYIRALLAGMISGLIEEWVHRGMNDLPESLN
                     IHSMLKEALRSHE"
     gene            complement(17048..17185)
                     /locus_tag="PLF14_01385"
     CDS             complement(17048..17185)
                     /locus_tag="PLF14_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT69987.1"
                     /translation="MCKQRPQNQKDESKVFEDLLSASESSMDFWFNDVDDEQWNDEKN
                     D"
     gene            complement(17199..17657)
                     /gene="rlmH"
                     /locus_tag="PLF14_01390"
     CDS             complement(17199..17657)
                     /gene="rlmH"
                     /locus_tag="PLF14_01390"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOT69988.1"
                     /translation="MKINIICVGKLKEKYLIEAQDEYVKRLSRFCDVKITEVSDEKDD
                     IPDAMEKEGARILSKIQPKDYVICLAIEGMKFDSEAFSEKVISVFDLSNPVIDFVIGG
                     SLGLSDSVKKRADMLLSFSDMTFPHRLMRVILLEQLFRAFKIANNESYHK"
     gene            complement(17654..18445)
                     /locus_tag="PLF14_01395"
     CDS             complement(17654..18445)
                     /locus_tag="PLF14_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT69989.1"
                     /translation="MIKFCPLFSGSSGNAIYLKYRDTSILIDAGLSKRKITQAIADIG
                     EDASELDAILITHEHYDHISGIGVMERGYNIPIYANTSTWKEMPRIFGRIREENKKTF
                     ETGKRFVIKDIEIMPFSIPHDTADPVGFSFFLDSKKITSVTDMGHLTETIAENIKGSD
                     LLLLESNHDLDMLINGRYPLKLKKRIQGEKGHLCNKDCGDIVVKFIEFGLKTVVLGHM
                     SAQNNVPQLAYDTVRKCIEENGYVIGKDINLVVAVREGNNRVFLL"
     gene            complement(18445..18981)
                     /locus_tag="PLF14_01400"
     CDS             complement(18445..18981)
                     /locus_tag="PLF14_01400"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOT69990.1"
                     /translation="MDIKEKITDRKVMYKGRILEVEKVTALLPDGKEVYRDVIRHSGA
                     CAIVALDEDMNIILEKQYRIAFDDILIELPAGKKEEGEEPLQCAVRELEEETGYVADK
                     MTHMITVASSPGFSDEVVHIFLAEGLTRTQMKWNQEERLIVWKEPLSDAKKMVMEGKI
                     KNGLAVVGILTACQLKGI"
     gene            19112..19795
                     /locus_tag="PLF14_01405"
     CDS             19112..19795
                     /locus_tag="PLF14_01405"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HOT69991.1"
                     /translation="MTGVFICLAIFFITLCVYLYHIIFKENPREKITKTAPSIMFFAT
                     AVVSVILTGNYTATALFLCAGLFFSIFGDFFLISEVKIKNSFLIGVAFFFIAQICYIV
                     SFSILVPITWLDILLFIGLEGSILLVYRFMGYDAKELKIPMLLYSSAISFMTVKAVSL
                     AFAKPFGFYHGITVVLGAVLFLFSDWLIARGILTGGRTLRARVLNLVTYYLGQIMLAF
                     SIFLMFMKY"
     regulatory      19889..20017
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            20105..21364
                     /locus_tag="PLF14_01410"
     CDS             20105..21364
                     /locus_tag="PLF14_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOT69992.1"
                     /translation="MNVLLKIAIVLVVGIIGGRVARLVKLPNVSGYLVFGLLLGPSFF
                     NFISPADVQSFNIINELALAAIAFSIGNEFVLKEMKKIGKSIFIITLMEVIGAVLLVF
                     CILFFVFRQPFAFSIVIASMSAATAPAATLLVMKQYNAKGPLTRTILPVVALDDVFGI
                     VVFGIALSVAKIVTYKQQLSVVSMMFGPLIEIAGSVGIGFVLGLLLSWLSQKASGRDE
                     LQAIALAAIGVATGISLLLNMSPLLTCIVMGTVLVNIQQNSIRVFDSVNSFAAPIYIL
                     FFTLAGASLDLSILKTVGLMGIAYVLARGLGKIAGAWAGAAMVKAEPAVRKYLGLGLL
                     PQGGISIGLMVLVRQQLPDYAVAINTIIMFSVLIYETSGPIFAKIALKKAGEIGIEEK
                     VRKPRRKGKKKSKVSSQKSESCIIDKG"
     gene            21369..21722
                     /locus_tag="PLF14_01415"
     CDS             21369..21722
                     /locus_tag="PLF14_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT69993.1"
                     /translation="MYVLFIVLNETCCLEDILAGFLREGIGGATIIDSQGMGRAIAHT
                     KNEDIPMFGTLRMMLGNTHPYSKTIFTVLNDEQMVEKAVSTVRETVKGCTCSGVGLMF
                     TVPIGKVYRMVEEDK"
     gene            21931..23394
                     /gene="malQ"
                     /locus_tag="PLF14_01420"
     CDS             21931..23394
                     /gene="malQ"
                     /locus_tag="PLF14_01420"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOT69994.1"
                     /translation="MRASGILLPVFSLPSPYGIGTMGKAAYEFVDFLVSAKQKYWQIL
                     PLGPTGYGDSPYQSFCSFASNSYFIDLDILESKGLLLKEEYQNEPWGENPDKVDYGLL
                     YRKRNKVLEKAFNRFDKENLSFKAFCRDNAYWINDYAIFMSLKEHFGGKEWQSWPEDI
                     RMRHNDALVKYGTHLSEKILFHKFLQFEFFTQWTQLKKYANEKGIMIIGDIPIYVSPD
                     SSDVWSRHYLFLTDEDRNLTSVAGVPPDAFSATGQLWGNPLYNWDAMEQEDFYWWKQR
                     TGMASLLYDYVRIDHFIGIERYFSIPAGSDTAATGVYHQGPGMKLIDAINQSRGNMGI
                     IAEDLGVLTDNVRKLLEKTGYPGMKVLQFGFSGEPSNDNLPMHYKKNLIAYTGTHDND
                     TILGWYKKLDRKNKDFLRKYLNRSRVHVYDLIFETMKSVANTAVVPIQDWLCQGSKSR
                     INFPSVAFGNWTYRITDNMLTESLAQKISDMTTLCAR"
     gene            complement(23399..25081)
                     /locus_tag="PLF14_01425"
     CDS             complement(23399..25081)
                     /locus_tag="PLF14_01425"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014522055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOT69995.1"
                     /translation="MTYKDKYNDWLKKADSDTIKELEAIKGNEKEIEDRFYKTLDFGT
                     AGLRGVMGAGTNRMNKYVIGQTTQALARYVIEQGMEKRGAVIAYDCRIKSDEYAKQSA
                     TVLAANGIKVYLYESLRSTPQLSYSIRKLNAATGINITASHNPKQYNGYKVYWEEGSQ
                     ILDDIADAIAENISRIDPFNDVKTVDFDKAVKSGQITMLGKDMDDMFIEEVLSLTLND
                     DIDKDVKIVYTPLYGTGLVPVTRILRERGFHNVTVVEEQKEPDGTFPTTPYPNPENVK
                     VFEIPLKYGKKTDADMIIASDPDCDRIAMMIKNDNNEFVFCTGNQTGALLAEYMLSQR
                     KEKGLYSPKDAVVQSIVSGSVVPKIAKHYGTHFFETLTGFKYICGLANEFEKTGEYRY
                     VFGYEESIGYCIETFTRDKNGVSAAMMMCEMAGYYKKRGITLYEVLTDIYERFGYMTE
                     NQISVEYPGIKGAKVKKHVMSQWRKEPLLEINGIKCSVCKDYINGYEGLPAQDALKYE
                     FEDGSWYALRPSGTEPKLKVYINAMGHSRKEADDLVKVFTEDISDYLAKCEK"
     gene            complement(25078..26106)
                     /locus_tag="PLF14_01430"
     CDS             complement(25078..26106)
                     /locus_tag="PLF14_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOT69996.1"
                     /translation="MGKIVTFGEIMLRLKSPYGERLLQSDKLEATFGGGEANVAVSCA
                     NFGMDTSYVTVLPDNDIAKACVRQLRGFGVNTDDIYYRNGRMGIYFLEPGAVQRPSKV
                     IYDREYSAISLVKPGDIDWKKVFNGASWLHVTGITPALTQNSALVTLDAVTAAKQAGL
                     TVSIDLNYRGNLWKYGKKASEVMPGIVKFTDVCVANEEDVQKTLEIHNDADVTKGELD
                     DSKYKELSDKVLSTYPNIKVIAITLRESRNANINGWGACINDRKDFYVSKKYEITDIV
                     DRVGGGDSFAAGLIYGLNTYGDSKKALEFAVAASCLKHTVYGDFSRFTVAEVERLMKG
                     DGSGRVQR"
     gene            complement(26099..26752)
                     /locus_tag="PLF14_01435"
     CDS             complement(26099..26752)
                     /locus_tag="PLF14_01435"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HOT69997.1"
                     /translation="MKKLQVIENVMKSGLIVVVRAQSAEEAYRITDACLEGGVKLIEI
                     TFTVDGAHRIIEELSKKYDEKDLILGAGTVMDEETARIAILSGAKYIIAPHLDLPTIT
                     MCHRYRVPAMPGCMTPREIVTATEAGVDIVKIFPGEVLGTKFIKAVKGPMPYLNLMVN
                     GGVDENNAKDWIKAGACGVGVGGNITKYAKTGEYKKMTAIAGQILESIKQAKEELNG"
     gene            complement(26794..27414)
                     /locus_tag="PLF14_01440"
     CDS             complement(26794..27414)
                     /locus_tag="PLF14_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008768185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOT69998.1"
                     /translation="MKRETLEVFEELLRSYTSLKKCKADIMRAFEITEETFRYGKMLL
                     ICGNGGSASDSEHIVGELMKGFNKRRPIEPNPMLDQSISERLSEGLPAICLSSHTSLI
                     TAIGNDASFEMIYAQQVYAFRKLAGVILCISTSGNASNVNNAAITARACRIPVIGLTG
                     ETGGQLNSLADVCIKVPASKTYKVQELHLPVYHCLCAMLECEFFED"
     gene            complement(27430..28419)
                     /locus_tag="PLF14_01445"
     CDS             complement(27430..28419)
                     /locus_tag="PLF14_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016206677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOT69999.1"
                     /translation="MYIAGIDIGGTKCAAVLGKCVDNNIIRVDIVDKISFKTKSCDSP
                     SAVIDRLILGLESILAKNFLISNDLSCIGISCGGPLDSEKGLILSPPNLIGWDSVPIT
                     DIFEERFKVKAVLQNDANAGALAEWLWGSGFGYRNFIFCTFGTGFGAGMILNGKLYTG
                     TNNMAGEIGHVRLADYGPVGYGKMGSVEGFCSGGGIAQLAKSMVREELQSGGKVSFCS
                     SETEIEQLDALIVARAAAKGDNLAKAIYARSGEMLGRALAILVDVLNPDAITIGSIFT
                     KERNLLWPHAKRVLSKESLAISSQVCDILPSSLGDAIGDYEALAVAADYVANN"
     gene            complement(28495..29568)
                     /locus_tag="PLF14_01450"
     CDS             complement(28495..29568)
                     /locus_tag="PLF14_01450"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT70000.1"
                     /translation="MLDTSVYALNDAQLRRYEELEKEYMQMFTDPKNCIPKFTIPIKT
                     SFNPTWEEILADKDVMLKNHLERNARHVEVGDDSTLNVRVDFGTCVVANAFGCEVFYP
                     TNSLPCAKDHVVKTREQIFSLKAPDINVAPYRKVMEWSEYFVEKLPEGYHMEFPDIQG
                     PFNNAHLIRGNDIFFDMYDDIEAFDKLMEVVTDATIEYAKATRQWTNAKEGWLYDWSA
                     LWKGNARISNCSLHMIGRELYLNHVIKHDVRFIRELNGARIHYCGSAKNVIEEMAKIP
                     GITGIDYESLLHDIEETMENVPEDLTILQPLGLSTDVAKKILSSKTWPFKKRNIIFRL
                     RGPDKTVEEAKELYRRLRRVAEN"
     gene            29747..30997
                     /locus_tag="PLF14_01455"
     CDS             29747..30997
                     /locus_tag="PLF14_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT70001.1"
                     /translation="MNWIDRTITNHLLHAMDFFPAVLLTGPRQVGKSSLLQMVAKDFE
                     YITFDNALILQQAKSDPELFLMSHSPKLILDEIQYVPELFSSLKIRIDNMRFEALSNN
                     KPRECLYLLSGSQAFELMQNVSESLAGRLAILPLQGISYREQKKISCALPFLPTEQYL
                     SVREKESVDLSDLWEIIHRGYMPRLVSSNDDWETFYASYVSTYIERDVNKISHIDDKT
                     DFVRFMASVAARSGELLNYDSISKDIGVSSTTVKRWLQILQTAGIIFLLYPYYSNHLK
                     RMIKTPKVYFMDTGLMAWLTRWVTPDTIKYGAKAGQFFETFVVSEIIKSLLNAGKSTR
                     DLYFYRDADQKEIDLIIQDGRTIYPVEIKMTARPHKKMASAFSVLESVRADDIQIGNG
                     AVINLYHKPLLIDRNVLSIPAGYL"
     gene            complement(30999..33350)
                     /locus_tag="PLF14_01460"
     CDS             complement(30999..33350)
                     /locus_tag="PLF14_01460"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOT70002.1"
                     /translation="MRIHINDDWQFYPSVESSESFTVRLPHSNVDMPYNCFDEAIYQK
                     ISKYEKIIHADNSWENKTILLTFSAVAHEAEVYINDRHVYSNSCGYTSFTVDISQYIE
                     WGKENRITVIADAYETLNQPPFGNVIDYMTYGGIYREAYIDIKEKTHISDVFVSGKAK
                     AADGLCEFFAEVQIVNCTEDCEIQLSIFAVKDNECVFEKYYPVRKEIIHLSEKVPAEN
                     WDIDNPNLYRITVKLYRNKKLSDEYCDRFGFRDAIFKNDGFYLNGKKVKVTGLNRHQS
                     YAYTGYAMSKRVQINDADILKNMLNVNAVRTSHYPQSKHFINRCDEIGLLVFTEIPGW
                     QHIGDEQWKKKAVENTENMVKQYRNHPSIILWGVRINESPDDDDFYRQTNKVAKQYDS
                     YRQTGGVRCIKKSSFLEDVYTYNDFTHNGTKQVIAKKKDVTKGDVPYLITEYNGHMYP
                     TKMFDDQPHRTEHALRHARVLNAVMGDDGVAGSFGWCMNDYNTHKDFGSGDRICYHGV
                     LDMFRNPKDAAYVYASQTDKYPVMHVATSMSKGDYPMAMTKSVWAFTNCDYIKMSING
                     KHIKDFYPDRKRFKHLLHPPIEIDDFTGNMLIDDLHFSEYSSRVIKKLLSYIYVNGPE
                     NLPLGYMLMLVTVLIKEKLTINKLTEIITAHVAGWGDNVKQYVFEGIKDGKTVITEIK
                     EPMNKMQLVLSADAHELKEEETYDTASVTIRAVDQNMNLLNYCNEPLRIVTEGPIKLI
                     GPELISLKGGCAGVYVKTDKQSGKAVLRVIGKDVTAETEFSVS"
     gene            complement(33366..34562)
                     /locus_tag="PLF14_01465"
     CDS             complement(33366..34562)
                     /locus_tag="PLF14_01465"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT70003.1"
                     /translation="MSLKKVTLRLNIKYGVMQALYWMGFITVYNYAAYFLSDKGFNSS
                     QTGIILAVASILAALLQPPIAAYADKAEESKLKRIILALTSAIVVLTVLIVAIRSYPY
                     LTAILLLLIITFVHTLHSFINSFAMEYANKGIQLNFTIARAVGSLGAAFASFGVGYIT
                     AKLGADSVPVIYFAMFLLLIPVTLSFKPAHDVGISSEVIKYAHAPATGVFKFFAKYKK
                     FTGLLIGSTLIFTSHNMNINFLINIVKRLGHGSAELGNVLGIAAIFEVIGMILYLIIS
                     KRLKCSMLVQISAVFFTIEKLGILLSKNINHIYASYSLQMFSYAVFVPATVYYINNLM
                     RDNDRIKGQAYLTTTMTLGGVFGSLLGGTLIDALSIPSMLLIATIISAAGSLLVVVFT
                     EKEKAI"
     gene            34816..35385
                     /locus_tag="PLF14_01470"
     CDS             34816..35385
                     /locus_tag="PLF14_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT70004.1"
                     /translation="MMLQELLERENMTLYKLSKNSGVPYTTVNDIYHGRTGLGKCTAE
                     TVYKLSKALSVSMEELLLPYLIERTDFELYKSSVCHRLKESGDMAFIAETLQQDNISK
                     LYRRKMYPESLYLLAMLDYVSRENNIPLCTKYDALRNVKLEETLYPAGVLVMNTVIGN
                     NKIKEDALSQAIPEFMRHNIVESEVRNVI"
     gene            35375..36106
                     /locus_tag="PLF14_01475"
     CDS             35375..36106
                     /locus_tag="PLF14_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HOT70005.1"
                     /translation="MSSDTVYSFNKDNLDIYLKELAKLFRKQNGKKATAEIILVGGAA
                     VLVNYGFRNMTADIDAIIHASSSMKDAINQIGDKYNLPNGWLNTDFIRTASYSDKLNV
                     FSVYYKTFYGVLQVRSISAEYLIAMKLKSGRKYKNDMSDIIGILAEHKERKEPLTWEK
                     VNQAVVDLYQDWDGITEEVKVFIKNAIDTDNYDTVFQQIKEQEQLSKNILIQFQQEHP
                     HTVTESNVDSILRMLESKKTSDALE"
     gene            complement(36278..37981)
                     /locus_tag="PLF14_01480"
     CDS             complement(36278..37981)
                     /locus_tag="PLF14_01480"
                     /inference="COORDINATES: protein motif:HMM:NF021375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HOT70006.1"
                     /translation="MAIMIPEKPHQFDSVSLEGLMFDALKLLPDDYYVFHSFRIASVT
                     ENILHESETDFVIFNRRKGVLCIEAKAGQVIYENGYWYYSSGEPMHNGGPFNQASSNK
                     YKLIRYIERSSCSGILSRCKFLHGVWFPSVTDIRLRTMTLPPEADRNLIMTKEALESP
                     DEYLNKIFSTELPNGITTDLSEYEEKKIIREIFCPQFNVFPSASFENDLKKIVFHRFL
                     KEQEGILNFLTEQRTAVINGVAGTGKTMIAIEKAQRHASSGEKVLFLCFNNQLKTHLS
                     KCYQNEKIDFYTVAGLACKVCDTAVPDYKRFKAALEDMYLSESFPYQHIIVDEGQDFG
                     IDTINEADILQLMKDIITDTKPLGTFYVFYDKLQMIQANHFPKFIEEADCKLTLYKNC
                     RNTENIATTSLRPITVKKPLLIEGCLKGVPAKIHFCSSPESVLARIDHTINNLNKDGF
                     SDIVILTCKTEEESVFNSKCKDGLYRNKYRFTTCRKFKGLEADAIILIDVDSDSFNSK
                     NVLIYYVGTSRARLKLDVITQLSDNDCIDILVHNLGYTGKIKRPKKELASALNAIGSI
                     V"
     gene            complement(37983..40334)
                     /locus_tag="PLF14_01485"
     CDS             complement(37983..40334)
                     /locus_tag="PLF14_01485"
                     /inference="COORDINATES: protein motif:HMM:NF038324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HOT70007.1"
                     /translation="MELENAERIRLNHITHSVRASQAVLQYGVGAMVDFKDQTLMTAA
                     PEYWREQIIEIHDERLEKALRVDFFGIPAGKNNELCKEGISYARFPEWYFCPKCRKFQ
                     PIKHWIDEYKRKGRKRAIESDPYMVKHMCCPICNQDLVVARIVTVCEHGHIDDFPWVK
                     WVHCKNSGGAKIICNRPSLTFKTGASSTEGLEGLVITCETCGAKTTLKEAFDREIFEE
                     LDKKHNNYYNFTCSGRHPWKNQKESCSLYPKVLQRGSSSVYFPVIVSSLVIPPYSSML
                     TRKIEESSAFSECKNVISAFMRNPYTAQESKDAIIVNQIDHYSNQMSLEINVSPEKIK
                     QVLERKWRQPSEEEYSTLSVKYRAEEYDALSGEISLGKDIYGDFQRESTDIVAYNLPY
                     IKRISLIHKVREVQALTGFSRIRPVDRTEPVGKQVNVVPIKEPTTNWYPAYEVRGEGI
                     FIEFNQEAINNWRSENRDLQLRIDRLNENYSKSFISSGNPRTVTSKYVLLHTISHLLI
                     KQLSFECGYSIASLKERIYCSEGSEGKEMAGILIYTASGDSEGTLGGLVRQGYPDTFP
                     NIFKKAIKSSEVCSNDPVCSLSLGQGRDSLNLAACYSCALIPETSCEEFNIFLDRGTV
                     AGVINNYRLGFFYEQLHGNRRWESFVTHTEDSSANRKTNLQVIIPEKGTDMSDVSYAD
                     IWKSVKEWTDNLTERSLLDKLINIADICDSKEKPVYECSFIIAGDSKKYRATLLWEKS
                     KVMYFSSDYKDTFEKITTEKWKCFCGDNKIIDATDILNAIGGK"
     gene            complement(40336..44025)
                     /locus_tag="PLF14_01490"
     CDS             complement(40336..44025)
                     /locus_tag="PLF14_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018885348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOT70008.1"
                     /translation="MANADEIKLLIEARNEYVKLIKQEMLGPGSEISIPDEEHELITS
                     SPDIRYSIGILFPRNNMINADNDDSVRVEESVSEENEEVFEEDLNSEITNEATSYKGD
                     SVNPADEENLDEEIGLAAQNMPSSMGITFFALGDSSEIKCKITFATYIRAELNDCRIP
                     FSPQNPETYTVPEPLSNYLVYDVSERCLKFKDSKISRREIHNLKKQNLLKFDESNIYD
                     LMYKLADQGQRGFVRVPHSLEVKLKFGNSDYIDKNKELDGTKAKLTALRKRISDDLYS
                     ITIMLVNDLEGKSNGLRCVFQPEIRVSSYDNSFTFRDYSGVAEFSALDSEEQSLELQY
                     RNKKVYGTGLGTSINWNVDGNGNGELFSDFFPMLEVPQMDFELPPKYAVEKKTLSMRY
                     LSDFCDSPKEEKIELLNIFADAYKKWIDELTEKLSVIDAEYNGRYNKIAETNITKCRI
                     SYNRIKKGIKALESSEMKWNAFALANRAMLMQRIHLKLQEVTSDIDRYPDDPEISDML
                     NKLDYGDNKDITADKYFWRPFQLAFLLMSINSVSEDASAERDIVDLIWFPTGGGKTEA
                     YLGLTAFTIFYRRLAHPDSYAGTSVIMRYTLRLLAAQQFTRASTLICACEYIRRDSLR
                     RNPRYKSYPLGEDDITIGLWIGGEHTPNKNKEAKDCLEKLIKSTADNIRENKDKYNKF
                     QVLKCPWCGTKLVKDKNRINQRLDGQFGYRMRNGNYFELFCPQESCFFNQVGKLPIQI
                     IDEELYKSPPTLLFGTVDKFAMLPWKKEIGSFFGLNNSNRSPELIIQDELHLISGPLG
                     TMVGLYETAIDALCSSKGIKAKIVASTATIRRAAEQCSALYNREVAQFPQPGLDAEDS
                     FFARELNINYDINQYGRLYIGLMPSGKTKAMMQVRSIAALLQKINVMDLPDSIKDKFW
                     TLTVYFNSLKDLGKCSTLVDDDVKDFIKRTAYRLGTAKDARMIAGADELTSRVSTTEL
                     NETLDKLEKLEYSKENIENKRYASNVLLATNMISVGIDVARLNVMLLVGQPKLTSEYI
                     QASSRIGRSNPGIAFILYDGSKSRDRSHYEQFKPYHESFYKYVEPTGATPFSNPARER
                     ALHAVVIALLRHTNTDLLPENGAANFSKEKYTDSIEKIRDYIINRDASIITRMNSGMT
                     SDSVLISEEIDRIVDEWERMAESYGKDCFTYGEKYMIKSPKDGEARLMKVYNSGYYDA
                     AFDTMTSMRNVDSLVKGNILIWEEK"
     gene            complement(44037..46649)
                     /locus_tag="PLF14_01495"
     CDS             complement(44037..46649)
                     /locus_tag="PLF14_01495"
                     /inference="COORDINATES: protein motif:HMM:NF038316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DrmE family protein"
                     /protein_id="HOT70009.1"
                     /translation="MKAINALKKIIERCDLLLEGNVISRELLMQTFADFLIETTNKEK
                     HNAGFVLHTGSVCYNAVAVVYAAATCLVYNEYDTEDIIASLQEGDLVLYKGKRYAYEG
                     YTKQDFFNRGKKEEYILISQNGSRDYVPKKLWGKIQPYFGKSQKLDGRGVHKKGTIRE
                     DFYKDVLNYKSEDIPAVINASVALVMPRSMADFLIDKLALSFNGKNIRLLDLITASYF
                     SENDEYHYRGNTAKTEAILKVSGKISVARQLIISREGNRHVGVIVFGNETVEKSLSEL
                     PELLNRKSLQYIYVMMNLDSENAITLIRQVENPNVFLCSKGFLLSNTLPCEIKNKYTR
                     ELEKQVSAVLDRQVISHSVEGWMNWEEYKIFREAIYDIKNSDYVTEEKENFIITAWFL
                     MNLFMTVAFKITEIDNCVSLGLLTISSVKQRLKDLHSAAASLPKQLQERARNVILLLE
                     ISYLYLSDKCEKEEYLRDLLEKNKDKRIAIIVPKTYYVTVMTKCGYYDIMDNKHFLTI
                     INANSFDNSVLYDLVIVMGNFVGKRFNAFKCSSSPHIETILYSFESNIYKYRAGKFHR
                     EEAELNSMNTASAGMSDEFITDETYCTDNVEDVEEVAQIEESIDDYISKLNEIAAFSG
                     IEAFENQSGYTPNADVIAAGIFDSGERIFFTKFYKAYVFDESDGIVNEVNVSDLKEGD
                     SLIFTHNTDETRDIVDTVLTKLVDERRVGSEIIESYSKSKKWKQILQKYMQITGLSAG
                     EIAKRMIRNGVNVQEATIRGWLDEDSHTVGPREVDSIKQIALLVGDEEMFDNAQNYFN
                     ACKTIRKIRRQILDWIGEAIIKKISGRTPKEGTLEAYIYDRIDSVAKILYLESIVFVE
                     RSVPMNLTNRPINL"
     gene            complement(46971..48074)
                     /gene="dcm"
                     /locus_tag="PLF14_01500"
                     /pseudo
     CDS             complement(46971..48074)
                     /gene="dcm"
                     /locus_tag="PLF14_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008802756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(48075..48488)
                     /locus_tag="PLF14_01505"
     CDS             complement(48075..48488)
                     /locus_tag="PLF14_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009757073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HOT70010.1"
                     /translation="MDRLTPEQRRKNMQAVKNKDSEIERKLRQELWARGVRYRKNVKT
                     IYGHPDLAFIGKKVVVFCDSEFWHGYDWENKKEEIKSRREFWIPKIERNMQRDAEVNK
                     RLESEGWRVIRFWGKDIKENVSACADFVVKCLEES"
     gene            complement(49182..49268)
                     /locus_tag="PLF14_01510"
     tRNA            complement(49182..49268)
                     /locus_tag="PLF14_01510"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(49232..49234),aa:Leu,seq:cag)
     gene            49410..51482
                     /locus_tag="PLF14_01515"
     CDS             49410..51482
                     /locus_tag="PLF14_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70011.1"
                     /translation="MNAKIKSYNNRPTIFIDDKPVPPIIYALTDWIGGRLSYDEITRF
                     SIKKFAAAGIRLYQLDISFQDMWFEDGTFDISIALKQIKGIIDVRPDAAVFFRLHINP
                     PRWWYKGKESEWVRYANTEVYPEPDIELARTYILNDLKPVPRASFASERWMNDMTIMV
                     RRFLEELYKSPLSEHLAGIQVANGVYGEHHYWAFMRNDPDLSEPMLKRYRKFKNDPNA
                     QIPGMEERYNPKDGIFFDPEKDSNLIDYLTCQHEAVAQSIIHFCKLVKETWKREIITG
                     VFYGYYVSMFGRAGLGGHLAEEMILQCPYVDFLSAPQAYNSNLRHIGGAGVARGLLES
                     ARLNNKLFLDEMDHPTELGVLHGGMKVYPPYESLQILRRNTLVSFLSGMGFWYYDFGP
                     FNTSGWWYEPYYLDEIKSLQKIYNKYYEKEYIKRADVLLVFDTKVQKYTALTENADPI
                     TDKACINVAYPMALRSGASMDTIYLSDLGKTNLDKYKCIIFMNTFALTDTQKQYIAKK
                     VYKKGRSIVWFFAPGYTDKKRNDIAFCKQVSGFDLDVIAPAPQITVQCKDFSYSVDNS
                     DKESRLNKLFVPKDGNILGYIGKTPALAHKTIDGCDVYFSSIPFTTPESFRYIFERSG
                     VHIYDSCNDAVIEGSNLLLIHAEHKGERVIKFRDSEITVDFQAGETILFDINTKAILR
                     RGEQGLTV"
     gene            complement(51530..53347)
                     /locus_tag="PLF14_01520"
     CDS             complement(51530..53347)
                     /locus_tag="PLF14_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70012.1"
                     /translation="MENKKKTGVTIIIIVSVVIVAAALLFIFRDSLFGKTERFNVTTS
                     NSDIVFKRSDAQEPLNMPYRYSKVLMDKRLAFKEEIVSINISSVRYEMSDKGLSLYNY
                     NDILKNPDSSNVEEVIGSIKYCKGITALSGIISDKKDSKITIYEGYSADLLAENFQHY
                     VIIPSTLSSHINEELPDNEKVLFIINPGTSGLAYFTVIGEYTTKSESDTLYLSYSGLS
                     TVVLGAQKDVPDHIDCMEINVNKQADLTNFSYFLSEYFADMNMLNQYEKRINRFNEPY
                     SYMFVNTVDLKPIDLSGEKSSEKNIITISRIDGKDKLEMSHVYADALINDYSRYSQYI
                     TDLNISTGRKGVNPSDYPAFTKVPSYSFSITLPGTYGREITDYLKKSGKNWPWFHQAV
                     TGVSEIERMKKGCEITFYTNYTSKDLAALRNEDYRKEKELEGDIKGYAIVPVAMHEAT
                     RNNRSIDHHIVTMSESDRSINFLEAMRVGFKIIGYYELPEETKDKVEKDEKEEKIIAV
                     YDKEGNKIEMRESVPDGYDTIFLTYTGYNDKYKKEAFKNEHIEAITIETRSDTDITEL
                     IKYLKQYFAPAEEAAGYVGQKNELGLAYEYCFTITKNAN"
     gene            complement(53880..55685)
                     /locus_tag="PLF14_01525"
     CDS             complement(53880..55685)
                     /locus_tag="PLF14_01525"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOT70013.1"
                     /translation="MLHIGTKHVDITDGFWKKRQDINKDITVKAVQNRFLETGRFDAF
                     DFTNEKIAHIFYDSDVAKWIEGVSFILHKERNSELEAFIDRIVDLIEKNRSDDGYYNS
                     HFLRKPEERWQHRSDHELYCAGHLMEAAVQYYKATGKDKFLKLMEDYADYIYKVFITE
                     KSAAFKTPGHEEIELALVKMYDTTGKKKYLELSKHFIDRRGVEEEPWIGSNYNRYNTQ
                     DFLPVRQQRTAEGHAVRASYLYSGMADIAYRYKDKELLAACKAIFSNIVNKRMYITGG
                     TGSSYLGEAYTLDYDLPNTKGYAETCAAIALAFFAQRMLLSDADSVYADTVERIIYNG
                     MLSGVSLDGKAFFYENPLEIQPVLNNRNVATTLTERNAVTQRKEVFGCSCCPPNVIRF
                     IGAINEYIYSYNKDTLFINQFISSNLEYENIRAEISTEFPNCGKVYIKVSGINKVAVR
                     KPYWCKYLKLVINDVRSKYSSKKGYIYINLPKGQNRIRLEMDITPRLVYSNPKVYDNS
                     HKTAVMRGPVVYCAEAVDNDFPLNNIVFGKELNYKTVKSDQLDAYMLDVDIKIPQSID
                     DLYTFTPFKYRKKTLRMIPYYCFANRGETEMRVWL"
     gene            complement(55730..58090)
                     /locus_tag="PLF14_01530"
     CDS             complement(55730..58090)
                     /locus_tag="PLF14_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70014.1"
                     /translation="MKKNTVRIGILLLVLTFSVITLASFPTDDAVIVNTEERVQSEQA
                     TVQQSATEEVIKGPWENAVKIYGNTTHSLSFSDSFSEKWFELEPVTEGKSVILTISCD
                     DQAATGVISALYYKDDVLSQGNNAERLFGTYMLNKPSAYKISKGGSYYLKVSVIGNSA
                     IGSNIKLNYTTIVNDIYEDNDVWERATPIDIKKPYIIDISAINDIDWFKISLQKDQAL
                     QYIIRNDISSGKGVNVALYREEDLIKNGDTAPVLFTSDEFNKYYTHKSDKTQTYYLKV
                     TPSAREAVLNKVTLITDIIPPDSLEGNDSYEQAVMLTPGVAKTVTISASNDVDWLMVK
                     TDKPGQTVKLSIRNASFSGAKLVCDWYEGARLITGSEAVETLYKTRGTGTSYVYMEEA
                     GTYYFKLYTVDNSVITTPIEISYTVIPADRYEPNNDYQSAKTISGNSETRFTLPALND
                     QDWFAVKTYKENQALRLNTYIPKTGASVNIFVFRQDDVESYGEAAPLFTFNSAKAPYI
                     YMLEEKGTYYIKIVSDTIISDDCILYLSLLNSDANELNNTRDTATYFPAGERKTFTIS
                     GVNDTDWFYFNVEKDNSVIDYIMQSDFNKYAKVSIYSEAELDSYEEYAAPLFTSDKYD
                     ERVAYKLDKAGKYYIKIDSALPVFDDIHLEYTIRESDKHENNDTWDKATPLTPTEKID
                     FTIRAANDADWFKISSSGMNKMSLTFNVSSKYMMKDVINIYVYREVDLLNYGSRAQHI
                     HVAKISDISNFIDIADGTYYLKAVAVKNDAFDFDYTLRANVFKMQD"
     gene            58278..58988
                     /gene="fabG"
                     /locus_tag="PLF14_01535"
     CDS             58278..58988
                     /gene="fabG"
                     /locus_tag="PLF14_01535"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HOT70015.1"
                     /translation="MRTVLITGASKGIGRQTTEDFLAAGYNVIANFHTTKMDKSEYPS
                     FQEHIEVIKADVSDYGQVEEMFKKGKERFGNIDVVINNAAVNYAGLLSQMEEKDWDRI
                     INVNLKGIFNVCKCAIPSMVNRKAGHIINISSMWGITGASCEVAYSVSKAGVIGFTKA
                     LAKELAPSNIYVNCVAPGIIQTDMNKAYTQQELEEMIPLGRIGKCRDVSEMLLFLADE
                     KTSFITGQVFSVNGGTVI"
     gene            59053..59445
                     /locus_tag="PLF14_01540"
     CDS             59053..59445
                     /locus_tag="PLF14_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70016.1"
                     /translation="MMRRIREFFMNRYGTDQLNRALMVVYALFFFVYLITKNNVFLFI
                     ALAVALYQVFRTLSRNFTQRAKENQFFLKYWNPVKRKYSSIKKKVTDKTHRYYKCPKC
                     KTEVRVPKNKGKIRITCPKCSEQFIKKT"
     gene            59552..60451
                     /locus_tag="PLF14_01545"
     CDS             59552..60451
                     /locus_tag="PLF14_01545"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOT70017.1"
                     /translation="MEFLKTLEQLRTPFFDQFFLLVTKMGEELFFTVVILIVIWCIDK
                     KTGYRMFFIFLLGSFVNQMLKLIFKVPRPWTYENAAKPVEGSVEAATGYSFPSGHTQQ
                     GIGLFGSVAMFLKKTRVYIVMVILTLLVAFSRMYLGVHTPADVFTSLAIGVVLLLLCY
                     YAFRVADKHKYGDVYLYGIVAFAIVISLIVLSFDKTGSPEYLHGIENGWKLLGCCIGI
                     IVSIIVDIKYTNFEVKAKWWQQIIKLVVGLGIVLVIKSFLKQPLLDLFNQSPVADGIR
                     YLLIILFAGCLYPMLFKVFKRKQ"
     gene            complement(60507..61601)
                     /locus_tag="PLF14_01550"
     CDS             complement(60507..61601)
                     /locus_tag="PLF14_01550"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOT70018.1"
                     /translation="MNATSSKIIRTIAILVIIFVVIGLGAWYVVEHGFSCFTFSFVDG
                     HRIEIRRGQYEEKARYETGTSGIKEIEVNWITKAVEFKVHNQDKILLIESCQRDLSKG
                     ENLTYTVKDGILKISELEDESIFRIAPKKVVIWIPKTLSESLSALKADGQISDISAQD
                     VTADTIYLETHTGRINVSNLAASRTITVITDTSSITASELKAKNIVIKSDTGNISLNG
                     ITAEEKINTETDTGRIEAKDIIAETLYCKSDVGRVICKNYKVKSVTQKSDTGTVETEG
                     SFRTGEFRSNTGSITMKCATPPESIYAETDTGSINLYTVKTDKLVVSYSTSVGKFDAE
                     IPVLVSHSADANINLKTSTGNIYIKQYEAE"
     gene            complement(61613..62239)
                     /locus_tag="PLF14_01555"
     CDS             complement(61613..62239)
                     /locus_tag="PLF14_01555"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HOT70019.1"
                     /translation="MENKLKAYVNNLFRDAPATAKAYEVKEEILQNLKDKYNDLVTEG
                     KSKEEAYKIAVESIGDLSELFAELEKEITPDDSKRQKYLALRAVVAMIFVLAAVPVVM
                     ALSYRSVLINIMIAVILVAIGVILAIYNRTNKPGYTQISTQTDRMAEIERKRSIRKSV
                     SGFLWLIILIMYFIISFQTHAWHLTWLIFLFGAAVECILDIWLGKAKK"
     gene            complement(62229..62576)
                     /locus_tag="PLF14_01560"
     CDS             complement(62229..62576)
                     /locus_tag="PLF14_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOT70020.1"
                     /translation="MTIASDLIRGNTDTIILASLMKKDSYGYEINKDISAKTAGEYEF
                     KEATLYTAFKRLEEGGFITSYWGEDNVTARRRYYSITERGKEEYIKLLNDWNKSKNMI
                     EKLIDTGVIVNGE"
     gene            complement(62950..65418)
                     /gene="gyrA"
                     /locus_tag="PLF14_01565"
     CDS             complement(62950..65418)
                     /gene="gyrA"
                     /locus_tag="PLF14_01565"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOT70021.1"
                     /translation="MENENLCDVSKIIPVDIEEQMRKAFIEYAMSVIADRALPDVRDG
                     FKPVHRRILYAMHLLGFYPDKSYRKCAATVGEVLGKFHPHGDAAVYDAMVRMAQDFSL
                     RYPLIDGHGNFGSRDGDPPAAMRYTESRLTKIAVEMLADINKETVDFKSNFDDHEMEP
                     VVMPSRFPNLLVNGSSGIAVGMATNIPTHNLTEVIDAIFAVIKNPDITIDELIEIVKG
                     PDFPTYGTIMGRSGIYQAYKTGRGRIVVRGKAEIEELANGRQRIIIHDLPYQVNKAKL
                     IETIADMVKNKSIEGISEINDESDRNEEVRIIITLKKDANANVILNRLYKHTKLQDAF
                     NANMLALVPSKTGYEPKTLNLKQMLEYYIQHQVDVIRRRTKFDLDKAQTRAHVLEGLI
                     KAVDILDAVIETIRKSPNEPEAKIRLVEKFEFTDRQAQAIVDMRLGRLTGLEILKLQT
                     EYDELTEKIKYYLSILEHEEITNQVVMEELEVIKKKYGDERRTAIEDSEEDLLDEDLI
                     KEETVVLTLTRFGYIKRTPVDTYRSQNRGGKGIIGVTTREEDNIVDIFTTSTHSTVLC
                     FTDRGKVYKLRGFQIPEAGRTARGTAIVNLLELDPNEKVTTILPIESFETNQNLFMST
                     KLGFVKKTPLKSYVNIRRSGIIAVTLREEDDLIAVRLSEGDDTVMLITNKGKAIKFAE
                     QDTKETGRSAKGVIGIRLDDDDYVVGMSLVKEEDTILSVTENGFGKRTFVSEYPIQKR
                     AGKGVINYKVTGRTGSIIASMVASDQDDLLLMSLQGDMIRLDVGQISILSRATQGVTL
                     MRIEEDNAVVAATITEKEQEEEEE"
     gene            complement(65625..66047)
                     /locus_tag="PLF14_01570"
     CDS             complement(65625..66047)
                     /locus_tag="PLF14_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70022.1"
                     /translation="MTEDRRNISAEEKTKAIRKAKIEKIVTISILVLLVLGIAAALIF
                     KSIVGTWWVEIQTDSTTRGAYIYFGGDKTYETFMYGVDEWAEKGTYELKGTSVNGYIV
                     FNPENGRQYVRQYNIRGTRLKIFNPDGSFVSFLRNRTD"
     gene            complement(66085..66888)
                     /locus_tag="PLF14_01575"
     CDS             complement(66085..66888)
                     /locus_tag="PLF14_01575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HOT70023.1"
                     /translation="MKEILNEILIPVSIVGGIALFFGVILTIAGKFFEVKKNEKAEAI
                     REILPGANCGACGYSGCDLYAEAVAEGKAEGNLCIPGTNASAIKIGEILGVKVATQKT
                     RVMKAICCPDVSKKYTYSGIMTCAAASLAYRGENSCLYSCLGYGDCVAACPNGAIMMK
                     DGAPVIDPVICTECGLCAKTCPRNLIRYLIGEEGYYVACASEDSGRVTGKNCKNGCIA
                     CKLCEKACEDDAIHVINNVAVIDYEKCTRCGKCYDVCPKKIIKKFCSSY"
     gene            complement(66899..67492)
                     /gene="rsxA"
                     /locus_tag="PLF14_01580"
     CDS             complement(66899..67492)
                     /gene="rsxA"
                     /locus_tag="PLF14_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HOT70024.1"
                     /translation="MNTDTFKMIIYISFGAILMNNFVLSNFLGICPFLGVSKKLSSAV
                     GMGVAVIFVMTLASVVTYLTNTYLLVPYNLEYLQTLTFILVIAGLVQFIEMFMKKALP
                     SLYKALGIYLPLITTNCAVLGVALLNVQYGYNLWQSALYGATGGLGFMIATIIFAGIR
                     ERLDMNDVPKYFKGLPIALITAAFLSMAFTGFKGMFG"
     gene            complement(67489..68151)
                     /locus_tag="PLF14_01585"
     CDS             complement(67489..68151)
                     /locus_tag="PLF14_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOT70025.1"
                     /translation="MKNRSLHELSKGIIKENPTFILVLGMCPTLAVSTTAKNSFMMGI
                     AATAVLVLSNIIISLLRKLIPDTVRIPVYITVIAGFVTIVQLLMKAFLPELDKALGIY
                     IPLIVVNCIILARAEAFAGKNNAWYSFLDGLGMGLGFTLALTVIGTIREFLGNGAIFG
                     FKIIKNIPMPAIFVTPAGAFLVLGSLMALVSALTRKKTEIKDCADCPVQCETKDKKGE
                     SR"
     gene            complement(68148..68681)
                     /locus_tag="PLF14_01590"
     CDS             complement(68148..68681)
                     /locus_tag="PLF14_01590"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOT70026.1"
                     /translation="MKKYIAPVLSLFLITLCVVGLLALTNRFTKGPIEAKEKEEAEKA
                     MNTVLPGGAPFTDISEKVMNLNINTKAKLKGVSKSDKGYVFTMVAKGYGGEMVVFVGI
                     NNEGKVENIVIGDNDETPNIGKNAELPEFTSQFTGLTMQDNIPDKIDYITRATVTTDA
                     VISAVGSALAIHAEVVK"
     gene            complement(68678..69598)
                     /locus_tag="PLF14_01595"
     CDS             complement(68678..69598)
                     /locus_tag="PLF14_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003365912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOT70027.1"
                     /translation="MDKLIVSSSPHIKDKATTSRIMLDVIIALMPTAILSVYYFSYVS
                     ALIIVASVSSCVLFEYLFNLITKKKHSETDLSCVVTGLILAMNMPPLKSSIILSIIGA
                     FIAIVVIKMLFGGIGQNIFNPAAAARIFLTTAFSSLMTVYVAPLTDGVTAATPLAIPK
                     GELLPSYLDLFLGKTPGSLGETAAVTLIAGGIYLMIRKVITWEIPVIYIGTVALLSFA
                     FGQDPLYSILAGGMMLGSIYMATDYTTSPVTRKGKIVYAAGLGLMTASIRKFGSNTEG
                     VAFSLLFMNALVPLIDKFTVKKTFGRIKKK"
     gene            complement(69591..70904)
                     /gene="rsxC"
                     /locus_tag="PLF14_01600"
     CDS             complement(69591..70904)
                     /gene="rsxC"
                     /locus_tag="PLF14_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOT70028.1"
                     /translation="MIIKIKNIKGVTLPHRKGTSEIPTETLPIPKLVEIPMQQHIGAP
                     CKVLVGKGDYVYVGQVLGDTEKFVSAPIHSSISGTVVQIKDMILHSGVKCESIVIEPD
                     GKQEVLPGITQPDISTKEKFLKAVRNSGLTGLGGAGFPAHVKLDTKERIPEALIVNAV
                     ECEPYITCDYRAIIEEGNEIVNGVKLILDKLSIPKAYIAVEDNKPAAFSLLKELAKDD
                     CRIETVMLKTKYPQGAEKMLIYALTGKEVPMNGLPIDIGTVVMNVTSVLALYKYCKTG
                     MPLVSKRITVEGNGVKNRGNYQVVIGTYIKDIFEYCNGDKEKTVKVLCGGPMMGTTAE
                     SYEMPVVKQSNAFLFMTPEDLDFMEESACIRCGRCVEACPMGLMPLTMNRYALAKMTE
                     ELEKYNIMNCIECGSCSYVCPAKRHLVQSFRVGKDLVRKAKVKNG"
     gene            complement(71004..71804)
                     /locus_tag="PLF14_01605"
     CDS             complement(71004..71804)
                     /locus_tag="PLF14_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70029.1"
                     /translation="MRRKTVIVIAIVLAIGFVVGLTSCTPYHKRPRPSINTANALEDK
                     YIIKEFPYDIATYGGDPIMKPTVSQFNNISRQKTLNARLSAFNEMIGRDPVQDRNEQL
                     YQTYEIRQMNHYLFSCEYFTGFETTVTNKGVNMIIGSEIDGTLLMNFPALLGTNQETD
                     AWEEFMIFFNNARTAVGAKEITKEYMATDNVSFVFDGDDVTNLTLRVLYKAPDDTEQQ
                     EVPMSLNEIKGCLSERMQVYMSFTEDASMTYEEYANTIAEYVLFLATE"
     gene            complement(72054..72848)
                     /locus_tag="PLF14_01610"
     CDS             complement(72054..72848)
                     /locus_tag="PLF14_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70030.1"
                     /translation="MKRKTVMLIAVILAIVFVTGLASCTPHEIRISPSINTANALEDK
                     YIVVEAEYNVATYGGEPIMKPTVAQFNNISRQKTLNARLNAFNEMVGRDSGQDRNEQL
                     YQSYEIRQINRYLFSCEYFTGFETTVTNKGVNMLVGSEINGTLLMNFPALLGTKQESD
                     SWTEFMVLFEEARKAAGAKEITKEYMATDNVSYVFDGDDLTDLTLRILYKAPDDTKQQ
                     EVPMKLNDIKGCFLEKIQIFMSFTEDPLMDYEEYANTILEYVLQQE"
     gene            complement(73254..73330)
                     /locus_tag="PLF14_01615"
     tRNA            complement(73254..73330)
                     /locus_tag="PLF14_01615"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(73294..73296),aa:Arg,seq:acg)
     gene            73439..74644
                     /locus_tag="PLF14_01620"
     CDS             73439..74644
                     /locus_tag="PLF14_01620"
                     /inference="COORDINATES: protein motif:HMM:NF018914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HOT70031.1"
                     /translation="MKDKFDSKSILYSDTLIPDIFLSEYLPLLSPQAVKVYTYCCFLE
                     KTERTVDIIKLSRRLDVEETALTSALEELTKKHLLSVSDKDIFVNDIKGIEIDRLYKE
                     RTSLKPDDLGEKASVISAINDQFFDGNMPIYMYGCIEQWFKKYRFEDPVMVMLFSVSN
                     EKGALTRNYIEAVAKDWFENGVKTVFDVEALFEERDKMKDMHTRIQKALNRKAPFTQY
                     ETDLINKWFNEYHYSFEIVEEALKKTVKISNPNIAYVDKILSTWYENQFKNLDDLEKD
                     KSIRDLSPDQLRMIIQEHYQGINMKNSMLFESRKAEVFKKSPQIEKLYNEINDLHFKQ
                     AFSPDKKAVAEEIKNKNYEMSLLFKHNNIPEDYLIRKYDCDICKDTGVDNGKDCSCKT
                     DFLRNYGTK"
     gene            complement(74649..76574)
                     /locus_tag="PLF14_01625"
     CDS             complement(74649..76574)
                     /locus_tag="PLF14_01625"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT70032.1"
                     /translation="MRIIAAHIYGYGKLADRTFNFKEGLNLVLGKNESGKSTLMSFIK
                     AMLYGHKKNEREKDGFIPEVKKYKPWNSDKYGGYLILETDEKKILRVERDFNGKTLSV
                     YNEYGEDITNEFSFSKENEMLGQDLLGMDYECFTNSSFICQDKSLLYAEDKEHIAQKL
                     MNINESGEEQISVGKAVKKLKEGITLLGNERTKGRKYNILSAQIREEEIKLTNMQNEN
                     KKCMEYLAEAEEIKKELEELEKEEKLCRKSETFKRVNENIEEYEGYNNEIEPLKKKLS
                     DTDIDAYEKRRKEFDTVSGEYKEPEKAYEKFVKEEKRNNTKITAFSILAILIIITGAV
                     LAITTDKLLLLSALAAIPFIVLLLITRKKNKETVNEKEKLDDIIKTKNELEQIETVLG
                     NDKSFNDLIKGYTGLQEDILRKEDVSDYNALLEKADIYNPEGKEPSMDYEECLNRIQT
                     KKVRLGVVKAQLDAYMNSDEQIASQKEHILLLEEQKKAVGEKQKALQCAIDGIEEASK
                     QIREEIIPEMNKNMSEYLEKITAGKHDNLSTGSTMDLNTEHNKTIRSVYSFSDGTLRQ
                     MYLAFRLAAAKVFSSECVTPVFMDETLVYYDEERKKSTFDFLYELSRHNQILFFATDM
                     ENFLLEGKDIAVIQLSK"
     gene            complement(76571..77617)
                     /locus_tag="PLF14_01630"
     CDS             complement(76571..77617)
                     /locus_tag="PLF14_01630"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT70033.1"
                     /translation="MVLTFFHMADIHLDQPFSSLGLVEGFPAKRREEIFHEFMRVLET
                     AREEQPAFLFIAGDLYEHGYTNIKRISKINEAFSKLKGNILLISGNHDPESKNSFYSH
                     FPWSENVHVLGRNKPNVIFEKEKVCVYGIGYDTGGGQAKILETIRTDRKYKNVLLLHG
                     DVDLSLSTYNAVSSDMLKDIGFDYVALGHNHRMYIKDNIFNPGSLCALSFKEEGAHGY
                     FKGDLSTGEVEFVNSKSRKYIDLKMDYEKLADFESMYPDKRNIYRIKVTGTKSDNKPL
                     AIPEGYDYVQLTDARTAKKEKKAQEITRGIKGRFTDIMTSKMENIPSEEIKIYEDALK
                     LGLQALSDEDLDLE"
     gene            complement(77589..77999)
                     /gene="acpS"
                     /locus_tag="PLF14_01635"
     CDS             complement(77589..77999)
                     /gene="acpS"
                     /locus_tag="PLF14_01635"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOT70034.1"
                     /translation="MRIACGADIVENKRISDAVSRSDRFLEKNFTPGEIKYCRSKKTL
                     MNQSLAARFAAKEAVSKALGTGFCATVQPIHIEVINDEKGKPHILLNEQLKARFPDIM
                     DISVSLSHCKEYSVAYAVITFKEEQDGSHILSYG"
     gene            complement(77996..78850)
                     /gene="ispE"
                     /locus_tag="PLF14_01640"
     CDS             complement(77996..78850)
                     /gene="ispE"
                     /locus_tag="PLF14_01640"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOT70035.1"
                     /translation="MIVQKAHAKINIGMDIKGLRQDRYHEIDTVMQTVELGDRITFAE
                     REKGIQFWCDDSSIKNNENTAFIAAKLFMKEMGIKRGIKIDLYKSIPLFSGLGGGSAD
                     AAAVLRGLNFLFNTNLPYEDLEKMAAMIGSDVPFCIRGGTQRATGRGEVLKPLKDFSD
                     VWFVIIKPEDSVETAWAYKEYDKAGNPVRPDMDLIESLLEERDIEGLKGNVVNIFEKV
                     VIPHKPSIQKVMDDLSETDAVTVFMTGSGSAVAAMYSEMRLASVAYTSLSRKHDDIFI
                     TKTSGGLK"
     gene            complement(78853..79884)
                     /gene="tdh"
                     /locus_tag="PLF14_01645"
     CDS             complement(78853..79884)
                     /gene="tdh"
                     /locus_tag="PLF14_01645"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF003808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HOT70036.1"
                     /translation="MKAIIKAAAGMGNVELAKRPVPEINENEVLIKIKKAGICGTDFH
                     IYKWDKWSQNRIKPPLIIGHEFAGEIVETGKSVKGLYIGQRVSAEGHITCQRCKLCRT
                     GRGHICEDVDIIGVDIDGCFAEYVKMPAINVWPLDDEVADKYGAIMDPLGNAMHTVMA
                     QPISSRDVLITGGGSIGLFATAIARKCGAARLVVSEPQPYKAALAKKAGADVVINPLS
                     DNPEQQVIEAFRGNRPEVVLEMSGFPSAFQLGLEVIENGGDMAILGIPSEELNINMGK
                     YVIFKGITLRGINGRRMFDTWYQCQNFLKGNSSFIDHVITHELPMEDINEGFRLMSES
                     KAVKVVLDF"
     gene            80035..80475
                     /locus_tag="PLF14_01650"
     CDS             80035..80475
                     /locus_tag="PLF14_01650"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOT70037.1"
                     /translation="MKNLFDKLMQNRLFRELFLLFKYGIVGIVNTGLTTGIFFLLSHL
                     GLKYYVYTVIGYIVGILTSFFLNRKYTFKKDNENITRQIILFFVISACLLGLVQLIQY
                     LLIDVLSLKEWLGVGIGMVFYTVTGFLLNRNVVFKYKDKENPEQ"
     gene            complement(80517..81830)
                     /locus_tag="PLF14_01655"
     CDS             complement(80517..81830)
                     /locus_tag="PLF14_01655"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT70038.1"
                     /translation="MQLKSKLSIFLLVIIVVASVFAYMGAILVSNKNFIETIKENQRY
                     VAETILSANKETDLPLERIIAITHNALYPVKLTKGIDDLRLSDTEKALLENGIIVFIK
                     QRVIPVGISVVKTDEYYLRVGYQPENNLFRNYAMLLAYAFLFSIVVFMLIIMGLSNKA
                     MKPIVQLNNATQEVAKGNFDVVVVNDSQDEIGQLTESFNKMAKELKSIEYLRKDFVSN
                     VSHEFKTPISSILGFAKLLESPNLTDEEKKEYLDIIVEESKRLSNLSTNILNLSKLES
                     QEIVFNKSEYSLDEQIRNVILLLEPKWYEKSISFDIEMEHISFYANEDLMQQVWINLF
                     NNAITYSHPKGVVHIKMYNEEEKAVIVIQDEGIGMDEYTKNRMFEKFFKGDTVHSKEG
                     TGLGLALVKRIIDLHNGAIEVESKPGEGTTIKLIFPNEFRGWEVI"
     gene            complement(81814..82500)
                     /locus_tag="PLF14_01660"
     CDS             complement(81814..82500)
                     /locus_tag="PLF14_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT70039.1"
                     /translation="MFNILIVEDDKNLRKLIGTVLKQNGFNVLCANDGEQALEVLDST
                     KADLIICDIMMPNMDGYELTESLRWSNYNMPILMITAKETLEDKRKGFLSGADDYMVK
                     PINTDEMLLRVNALLRRSKIATEHRIEVGDVIIDYDTLSVQKDTERIVLPKKEFLLLF
                     KLMSYPKKIFTRQQLMDDIWGLDSETDERTVDVHIKRLREKLVNIDEFEIVTVRGLGY
                     KAEKKNATEI"
     gene            complement(82599..83279)
                     /locus_tag="PLF14_01665"
     CDS             complement(82599..83279)
                     /locus_tag="PLF14_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOT70040.1"
                     /translation="MSNIFYMIIITKREFEEAYLNFFRENDVHMVLSDLCRGTAQKKM
                     LDYLGIEKTEKIMFHIFVDNDRREIILKNLVNQMEIEVAGNGIALTIPLESIGGESCL
                     KYLTEGQEIGKKKGEKMENLKYSLIIVIADKGASETVMEAARSANAKGGTVVSAKETE
                     VNANERFFGVSIAEEKEMIYILAKKQEKDEIMSAIMTKAGIGTDANAAVFSVPVDRIA
                     GLRSLMTD"
     gene            complement(83293..84840)
                     /locus_tag="PLF14_01670"
     CDS             complement(83293..84840)
                     /locus_tag="PLF14_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HOT70041.1"
                     /translation="MSRENREFIKHNRTILLEKLKESLSSVLPVTIVVFILCFIFFPA
                     DSGIMLTFILGAVMLILGMGLFTLGADIAMMPIGESVGSSITKSRKLWLIIIASLIIG
                     TAVTVSEPSLTVLAQQVPGIPDMVLILTVSLGVGLFLTFSMLRVIFRIKLAYVLMGVY
                     AVIFTLSFFAPKEFLSLAFDSGGASTGAMTVPFLLAFGAGAAAVRSDKDAHSDSFGFV
                     GLSAAGPILAVLLLGILYKPGNTTYEATAPVNPGKTTQITRLFITEIPGKLGEVALAI
                     LPIVVCFLIFQLAVLKLKKGQVIRIVVGLIYAYVGLVLFLTGANVGFLQMGSFLGKAL
                     GSLPYNWIVIPIGMVIGYFLVISEPAVHVLSKQVVELSSGAIPKKAIATSLSVGVATS
                     IGLCMIKILTSASILYFVIPGYVIGLILTFFVPPVFTSIAIDSGAVSSGPMASTFLVP
                     FAIGVCLSVGGNVIVDAFGIIALVTMTPFITIQVLGLFYRFKLKAAEREQEQQQKIKK
                     KESIINF"
     gene            complement(84940..85251)
                     /locus_tag="PLF14_01675"
     CDS             complement(84940..85251)
                     /locus_tag="PLF14_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HOT70042.1"
                     /translation="MDSRKFTMKDESFVCEACGVKVHALGVTARNHCPRCLYSKHVDN
                     NPGDRMCDCHGLMEPIGLEKYRDSYKIVFKCQRCGAVKKNIVAKDDNMDKIIELSSKP
                     V"
     gene            complement(85366..85456)
                     /locus_tag="PLF14_01680"
     tRNA            complement(85366..85456)
                     /locus_tag="PLF14_01680"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(85419..85421),aa:Ser,seq:gga)
     gene            complement(85485..86675)
                     /locus_tag="PLF14_01685"
     CDS             complement(85485..86675)
                     /locus_tag="PLF14_01685"
                     /EC_number="1.1.1.261"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-1-phosphate dehydrogenase"
                     /protein_id="HOT70043.1"
                     /translation="MKLKDLEIKELIKNIYPCECGLIHDPKMERIEISEHGTEEAIRF
                     ILNKAKGRIMVISDEITFKIAGKRVYERLKEVNKETYSYVIPQKNNIPVIPNESTIGE
                     TLIRMPLGIDFFVAVGSGVVNDVVRNISYHLGKEYAVIGTAPSMDGYASITSSLIINN
                     VKESCPGQVPKGIFADIDILQNAPYTMLTAGFGDVLSKYNAVREWRFSRDLNKEHYCE
                     EIAEMVWKAADKCADSASLLKNRDKKAVTGIMEALILSGIAMGMYKNTRPGSGCEHHI
                     VHYWDIEFIKRGWVHPLHGNAVGVALCVICRLYDLIKEKLPVEVTDLNTENIENILKS
                     AGCETNPKTLGIPEDIFHDSILYGYKMSSSKYTILTHLNKYDKALLEKCAQILTKQYY
                     IDIL"
     gene            complement(86685..87824)
                     /locus_tag="PLF14_01690"
     CDS             complement(86685..87824)
                     /locus_tag="PLF14_01690"
                     /inference="COORDINATES: protein motif:HMM:NF017495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HOT70044.1"
                     /translation="MLAKIIQLVLFFGIPYILYKLENKVKLIKTVGPIIFCYAIGIIW
                     ANSGLPLDAGLSDIVSSVLIPIAITLILFNSDFTTLRKNAKKAGLSFLILSVSVMVVA
                     AVSVLVFPNVYEKEKIASMLVGCYTGGTPNLFAIGYGVNADKNTIIVANLADQVLGGV
                     YFLFICTLAKPLLNKLSRQISVATEKTEYKKEGIVDKVEIPVKEKIKQGVIAILLGIA
                     ALGVSIGISYLLTKEATNVAIIMLGVSVIGVALSFVKGVRQNKVSYSIGQYFIYVFSL
                     AVGSMADLKLILGQSVYVLGMVAFVLFVTMFLHFFIALLFKIDADTIIITSIAGIFGP
                     AFIIPTAKAIKNEGVILIGIATGLLGYAIGNFMGFGLYEILKLIY"
     gene            complement(87853..89247)
                     /locus_tag="PLF14_01695"
     CDS             complement(87853..89247)
                     /locus_tag="PLF14_01695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOT70045.1"
                     /translation="MIKQFYQPEYMLRKHKKLGELPSAKEAYGRMLKMALPSTIEALL
                     GGLTSFVDMIMVSSVGITAVTAVGITTQPRMIILMLFLALNTGVTALIARRKGENDAK
                     RANRVLMQALLICFILAIIMTALGIVTARPLLRFAGAGEDFIDDSIVYYRIITAGTVF
                     GVLTMTINAAQRGAGNTKIAMTTSLISNVVNVFFNFLLIKGRLGFPALGVKGAAIATV
                     IGMVAAFLAACYSLRRATFIYFDIKGLKKFDLSIVKSIANISGNAALEELCFRFGFFM
                     YNKIVAELGTIDYGTHLVCMQIMNLSTYIGAGLGTGTTALVGQTLGEKRSDLSMLYIK
                     IGRRMAIIISVVLSAVFIFGGKMLMGLFTDDSVIIENGRYVLLIMALSTFCINTQIVH
                     SGSLRGAGDVKFVATMALISVAVIRPLFSYIMVYPLQLGLIGAWLGLLADQFVRSFSG
                     MLRIKSGKWAKIHI"
     gene            89446..91206
                     /locus_tag="PLF14_01700"
     CDS             89446..91206
                     /locus_tag="PLF14_01700"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HOT70046.1"
                     /translation="MTKNKMLLSWIEEMAALTTPDEIVWITGDSEQLDQLRQQAIATG
                     ELYKLNEEKLPDCYLHRTAVNDVARVEHRTFICSRREIDAGPTNNWMAPKEAYEKLGK
                     IFNKSMKGRTMYVIPYSMGPVGSKFSKIGIELTDSIYVVMNMVIMTRVGNDVLDALGN
                     SNDFVRGLHGKADIDEENRYICHFPEDNTIWSVNSGYGGNVLLGKKCFALRIASYQGK
                     NENWMAEHMLILGVEYPDGDTKYIAAAFPSACGKTNLAMLIPPEIFAKKGYKVWTVGD
                     DIAWIRKGPDGRLWAINPENGFFGVAPGTNIKSNPNALHATEKGTIFTNVVHNLENNT
                     VWWEGLDKNPPTKALNWKGEPWTAESGEKGAHPNSRFTAPATNCPSISKEFDSPNGVP
                     LSAMVFGGRRAKTAPLVYQSLDWVNGVFVGSIMASETTAAASGAVGVVRRDPMAMLPF
                     CGYNMGDYFQHWLNIGKDVGDKVPKIFHVNWFRLDDEGHFLWPGFGENLRVLLWILDR
                     CEDKVGAVETPIGYLPKPEDINLEGIDVTEETLKELLSVDKELWLEDTVRIEDFYNKI
                     GNTVPKELRNELTKLKKKLS"
     gene            91522..92844
                     /gene="dnaA"
                     /locus_tag="PLF14_01705"
     CDS             91522..92844
                     /gene="dnaA"
                     /locus_tag="PLF14_01705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOT70047.1"
                     /translation="MAVQEIWNDALTNIESVMGELNFKTWILPLEPYTIEGDNFVLIA
                     QSDFSKNYLANYKLLLKNAISDAAAKDLSPLFKLKNEIGEYDENGKHEDFRSNNYADS
                     QLLDKYVFSNFVTGSSNQFARAAAEAVVDNPGTTYNPLFLYGESGIGKTHLMHAIGNA
                     ISQKYPDKKILYVQSETFTNEMIFAIKSAKSKEFREKYRNLDVLLIDDIQFITGKEGT
                     QEEFFYTFEKLYYSNKQIVITSDVPPDKMTRLEERLKTRFQQGLMADMQTPDYETRVA
                     ILEQNSLKSEYNISKEVIEFIAKNISSNIRNLQGALNKTVAHARVFGEDITIDLAKKA
                     LKDMIDESNANDITIPSIIDTVAKYYNITTKDIISPTRKSAIAFPRQIAMYICRIKTP
                     FSLPVIGKYFADRDHTTVLYACDKIAKKIKTDKNVEKEVNELIEKLNY"
     gene            93045..94151
                     /gene="dnaN"
                     /locus_tag="PLF14_01710"
     CDS             93045..94151
                     /gene="dnaN"
                     /locus_tag="PLF14_01710"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOT70048.1"
                     /translation="MNFTCARKKLQDAVNSVSKAISSNPIMPVLGNINIKAKDSQIIF
                     RGYDLEIGIEHIEDAFIKEEGEILVNARLFSDIVRSLGEEEVEIFTEESKLVIISGNA
                     VFNIRWLDPAAFPKLPYVEKDNKVTIKTEYLKNMIRNTLFSVSTNEQRLILTGALFES
                     EENSFKMVTLDSFRMSIRKEYILGNKEEFNFVIPGKTLNELLKLITNDDEDIRIYYEK
                     TQVLFEFGNCKFISKLLQGDYFNYKAIIPTKDFATKIEINVNEFRNIIERNMLVSDDG
                     RKAPLILEIVENMLTANTQGEVGNVTEKCHIKKEGKDIRIGFNARYLLDAIKSIDEEK
                     VRISFMGETSACIITPLNSDEFLYLILPVRISAY"
     gene            94151..95257
                     /gene="recF"
                     /locus_tag="PLF14_01715"
     CDS             94151..95257
                     /gene="recF"
                     /locus_tag="PLF14_01715"
                     /inference="COORDINATES: protein motif:HMM:NF000823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HOT70049.1"
                     /translation="MLIERIVLKDFRNYEYQEISPNKGINIIYGRNAQGKTNILESIF
                     LCSTARSHRTNKDADLIRNLQQGYSVRIEGKRDEEPFSIDINCTKISSRQNKRVIKVN
                     DITLLKTGQLMGVLNSVMFSPEDLTVIKEGPSERRRFLDILISQINSSYFYSLQEYNR
                     IIKQKNALLKRVFETGKNSDLIDVYNIKQAKTGTKIIKERAAFTEQINEKAGQNHFDI
                     ANGTEELDIRYACDIPDYSGEESDFYKALDKIKDKEIKIQSTILGPHRDDVDILLNGL
                     NIRDYGSQGQQRSAILSLKFAEMQIIKDKTNKQPVLLLDDALSELDLLRKRKLLNKIE
                     TKQTFITCTEKRRYMTLDDKISFFYVSKGSVKSI"
     gene            95310..95579
                     /locus_tag="PLF14_01720"
     CDS             95310..95579
                     /locus_tag="PLF14_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HOT70050.1"
                     /translation="MFLHIGDNLVVREKEVIGIFDFETSTLSKHTKNYLRDKEKKGTI
                     TTVSDKLPKAFVVVKKNKESDFRIYLTLISSATLRKRAENNKITE"
     gene            95583..97526
                     /gene="gyrB"
                     /locus_tag="PLF14_01725"
     CDS             95583..97526
                     /gene="gyrB"
                     /locus_tag="PLF14_01725"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOT70051.1"
                     /translation="MDNKKRQDNIRDCNYDESQIQVLEGLEAVRKRPGMYIGTTSVRG
                     LHHLVYEIVANSIDEALAGRCDRIEITIDKGNIVTVTDNGSGIPVGIHPKMGIPTVEV
                     VHTVLHAGGKFDSSAYSISGGLHGVGASVVNALSEFLEVEVKRDGKIYKQRYERGKTV
                     SKLEIIGNTNETGTKTVFKADEQIFDTIEYEYDALLSRYREMAFLNKGVMIVLTDKRG
                     EEEVTNELCYEGGIVSFVRYLDSNKTRIHKDTIYIEAEKENCLVEIAMQYNDDYNETL
                     YSYANNIATSEGGMHVTGFRSALTKVINDYARKIGALKESDKNLTGEDVREGLTVVLS
                     VKLHDPQFEGQTKSKLGNSYVRTATEQTVNEELAAYFEENPQEGKVIIEKCIRASQAR
                     EAARGARELARRKTALEGNALPGKLADCSETDPTKSEIFIVEGDSAGGSAKQGRDRKY
                     QAILPLWGKMLNVEKARIEKVYNNDKLSPLITALGTGIGEDFNIEKLRYHKVIIMADA
                     DVDGAHIGMLLITFVYRYMRELIEKGHLYLAQPPLYRITKGKDSIYAYNEAEKDRILR
                     ERDWDSKDCTLQRYKGLGEMSSTQLWETTMNPATRTIIQIKMDNPFLADIAVSDLMGD
                     DVQPRKEFIERHAKDVQLNRLDI"
     gene            97558..98331
                     /locus_tag="PLF14_01730"
     CDS             97558..98331
                     /locus_tag="PLF14_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT70052.1"
                     /translation="MGKIIAVANQKGGVGKTTTSINLSASLAFMGKRTLLIDNDPQGN
                     ATSGLGVDKWTVKQSVYDAIINDIDIKETILPTMLDSLFLCPANIQLAGAEIELVSLM
                     MRENRLKNAIKPIRNDYDYIIIDCPPSLGLLTLNSLTAADTILVPIQCEYYALEGLKQ
                     LMKTVSLVQKHLNPSLKVEGVVMTMFDSRTNISLQVVESVRQHFSDRVYRTIIPRNVR
                     LSEAPSHGLPIILYDKDSKGAMSYLDLAEEVIQCGGENG"
     gene            98324..99172
                     /locus_tag="PLF14_01735"
     CDS             98324..99172
                     /locus_tag="PLF14_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOT70053.1"
                     /translation="MAKYGLGKGLGALLGGDYTDISVINKENQLIEMDIDKIQRDPRQ
                     PRRDFSKEEMDELAESVREKGIIQPILVRKTDDTYTIVAGERRFRAAKQEKLKTVPVI
                     VKEFTDKEVLEITLIENIQRKDLNPIEEAEAYKRLCDEFGATQEEIAKTLGKSRPVVT
                     NKLRLLNLEEKILSFIRRGKLSEGHARLLLSVHEDIREDLAEETVNKELSVRDLEKLI
                     KKTKRTPGKKEQIENDEEFAKVENKLQKKLATKVTITRSIKKGKITIEYYSLEGLNRI
                     IDILSR"
     gene            99194..99361
                     /locus_tag="PLF14_01740"
     CDS             99194..99361
                     /locus_tag="PLF14_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70054.1"
                     /translation="MEYLIYASISLGISLIVSSITVLIFVNKKKKNKKREQEREISID
                     NIKTYEDVLRQ"
     gene            99386..99688
                     /locus_tag="PLF14_01745"
                     /pseudo
     CDS             99386..99688
                     /locus_tag="PLF14_01745"
                     /inference="COORDINATES: protein motif:HMM:NF025935.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
     gene            99782..99872
                     /locus_tag="PLF14_01750"
     tRNA            99782..99872
                     /locus_tag="PLF14_01750"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:99817..99819,aa:Ser,seq:tga)
     gene            99876..99967
                     /locus_tag="PLF14_01755"
     tRNA            99876..99967
                     /locus_tag="PLF14_01755"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:99911..99913,aa:Ser,seq:gct)
     gene            100341..100475
                     /gene="rpmH"
                     /locus_tag="PLF14_01760"
     CDS             100341..100475
                     /gene="rpmH"
                     /locus_tag="PLF14_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOT70055.1"
                     /translation="MKRTYQPKTKQRNRVHGFRQRMKTAAGRKIIKNRRAGGRKRLSA
                     "
     gene            100516..100875
                     /gene="rnpA"
                     /locus_tag="PLF14_01765"
     CDS             100516..100875
                     /gene="rnpA"
                     /locus_tag="PLF14_01765"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOT70056.1"
                     /translation="METLKKNYEFLRVYRKGKYFSGKYLTINVLENAFLRNRVGISVS
                     RKAYKSSVKRNLYRRRIKEILRTGNFNLKNGYDMVFVVKKTDEIPEYHEIEKEIVDLL
                     DKAKLIIKNEKTDNQID"
     gene            100847..101056
                     /gene="yidD"
                     /locus_tag="PLF14_01770"
     CDS             100847..101056
                     /gene="yidD"
                     /locus_tag="PLF14_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOT70057.1"
                     /translation="MKKLIIRLIKFYRINISPNKLPCCRFLPTCSQYAIEAVEKYGAF
                     KGSLLSLWRILRCNPLCKGGYDPVK"
     gene            101071..102066
                     /locus_tag="PLF14_01775"
     CDS             101071..102066
                     /locus_tag="PLF14_01775"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HOT70058.1"
                     /translation="MFDFIYNFFGKIFELLYTGLCGQNFGITIIVYTLLLKLLLMPLS
                     IKGQKSTMRMTELTPKVDAIKRRYKNDQQKQNEEIQKLYKQENVGCASGCLPTLIQFP
                     LLIAMYRVLAQPLTYIKGVSKDVINQIIERLGYAPTVLQTTINNDLLSDPAKFELVKD
                     LISFDKLIDMNFLGMNLGLVPKVNPSILFGAETIGTYLPILFLPLISVGLVILSQYLN
                     DRAMKKRMPAKKKAPNPNDAPDMSEQMGKTMKFLMPAMTLWIGFTLPVSMSLYWIFTH
                     IVQIIQHFIMEKIRKNHDAKKEAEAVRSGAASQSEEIIEATYEVKEESKESKDKK"
     gene            102063..102713
                     /gene="jag"
                     /locus_tag="PLF14_01780"
     CDS             102063..102713
                     /gene="jag"
                     /locus_tag="PLF14_01780"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HOT70059.1"
                     /translation="MSRVSIEKTGKTVDEAVQAALLELGLELGDVEIEIVEEETKGLL
                     GLGRKEAKVRVYFSTEDDPKETIKFFLEDILSQMNLVPDVFVSEEDGFYKAEISCNDS
                     GLIIGRKGEVLQALQFITSQVVSRAYDTYIRVIIDTENYRERHKAYLEELARKTADKV
                     VKAKRDFMLDPMSSYDRRIIHTCLQDRENVSTYSIGDEPYRKIVISFERDGKRNSY"
     gene            102719..104089
                     /gene="mnmE"
                     /locus_tag="PLF14_01785"
     CDS             102719..104089
                     /gene="mnmE"
                     /locus_tag="PLF14_01785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOT70060.1"
                     /translation="MEHTIAAISTPVGVGGISVIRISGSKAFSVAESMFKSKTPFRDI
                     KSHTVTYGHIQDKKGNTLDNVLLLKMKAPKTYTKEDTVEIQCHGGLYVTRKILALAYT
                     NGAVPAQPGEFTKRAFINGSIDLMEAEAVMDLINSQAEDAYREALSQMKGEKSLQIKS
                     IRSELIDLISHIMVIMDYPEYDTEKITAKEAQDKAQDIADRLKVFVEDFDERRIIREG
                     LTVAVIGDVNVGKSTFINRACGENRVIVTDIAGTTRDVVEVKIDVKGLCVTLKDTAGI
                     RSTDNEIEKIGIEKSKQAIEQSDAVIYIADINQPYKDEILHYLRDRDKVICVLNKTDL
                     LLEKDKDKHIESMEKELKKLFKDRIIACSLDKKEGISEIFDKLLDMVNAGSIRSKDSA
                     ITNERHKKHILDAITNLENTKEGSFIDMLSIDLTTAAEELGKITGETASADIIDRIFE
                     KFCIGK"
     gene            104091..105971
                     /gene="mnmG"
                     /locus_tag="PLF14_01790"
     CDS             104091..105971
                     /gene="mnmG"
                     /locus_tag="PLF14_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOT70061.1"
                     /translation="MKQYDVVVVGAGHAGCEAALACARMGLKTAIFTLNLDSIANMPC
                     NPSIGGTAKGHLVREIDALGGQIGKCADATLIQSKMLNRSGGPAVYSLRAQIDRRQYQ
                     TVMKHMLEKQENLDIRQYEITDIIFDNNSGVTGVVTNTDVVISAKAVIIATGTYMKSR
                     IIIGDMSYEGGPDGFLPANRLSLNLEKKGIKLRRFKTGTPARVHKNSIDLTKMSVQNG
                     DEPIVPFSFETDKIEINQIPCYLTYTNEKTHDIIRKNLDRSPLYSGKISGTGVRYCPS
                     IEDKIVKFADKERHQVFLEPMGLMTDEMYVQGMSSSMPEDVQEAMYKSVKGMENVVFM
                     RSAYAIEYDCFDPLQLMPTLEFKKFPGLYSAGQSNGSSGYEEAAAQGIMAGINAALKI
                     KKQSSFVLDRSEAYIGVLIDDLVTKGTNEPYRMMTSRAEYRLILRQDNADERLTPKGY
                     KLGLISEERYTGFLRKMENIEKEIERLKKKNVSPKIVNPFLEKRNQQPLNSGTTEAAI
                     LKREGVGYNDLYEIDDEIPELTKEEREQVEIKIKYEGYIEKQQRQIEQFKKMEKVLLS
                     ENIDYKKIKGLRNEAQQKLDNVKPVSLGMAGRISGVSPADIQVLYVYIEQQKRKKRKA
                     KE"
     gene            105968..106645
                     /gene="rsmG"
                     /locus_tag="PLF14_01795"
     CDS             105968..106645
                     /gene="rsmG"
                     /locus_tag="PLF14_01795"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOT70062.1"
                     /translation="MKQLLKGIFKELGIEYQEEHYDKFDTYASLLIEWNKKFNLTGIT
                     DLRDIYIKHFGDSLLLYEGIKATESLIDVGSGAGFPGIPLKIAGYKQKTVLLEANGKK
                     ASFLELDNIFVCNKRAENAAREEAYRELFDVVVARAVAPLSILLEYCVPFIKREGIFI
                     AMKTDNSELQSAENAMKQLSVEILKEDKQTIPFSDIKRNNMYFKKTRNTAYKYPRPDG
                     VPKKKPL"
     gene            complement(106750..107490)
                     /locus_tag="PLF14_01800"
     CDS             complement(106750..107490)
                     /locus_tag="PLF14_01800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HOT70063.1"
                     /translation="MDILGIKIDNTDMEKALEKACDALSDRASFRYIFTPNAEICMAA
                     IKDNSLMEVLNSSFMNIPDGDGVVLASKILKTPLKEKVPGCVFVQNLLGMGKSFSVYL
                     LGASHDSVSTAAKKIQSLYPHITVAGYHDGFFNVDQEKEIIDNINTEKPDLIIVGLGA
                     PKQEFFIYKHRHNIHSGLAIGAGGTIDIISGFSKRAPEFFCKHKLEWFYRLLKQPARF
                     KRMLNIPLFLIKCRQHARKAFKKKNRRT"
     gene            107575..108747
                     /locus_tag="PLF14_01805"
     CDS             107575..108747
                     /locus_tag="PLF14_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70064.1"
                     /translation="MNFADMRKTNALKLIIIILIMSAVLVSCTEEEYRASRLYKSLTS
                     YEQKSETVTLKNKKYNKIDLSKKAVLELSKGMIYNVDFSEAVNVRDASTASILNSEII
                     SKTLAMKVTEKDTVMNVISTDIASYGDYVISVTDNAVFTGSGLKVNNLGEGGAAIKIS
                     SGASMVLNKSNVKANGAGVESDSLVSISSSEMTVESLRFYEGATVTLDDSRFYTNHGI
                     MLLDNANEKLIHVSLNLKKAKLTADDGALLSMIDTKASVKIEDTTLNQGSSNIMLLKN
                     SEATVTLCNSDAEGGVMTDESSSLNLLIKNGSAFKGYINKGNRTKTVTVQIEENSVWE
                     VTSDSYVRGVILKDANFENIKSNGFTIYYDPKSSTNAWLDKKTVNLPDGGRLAPLR"
     gene            complement(108764..109426)
                     /locus_tag="PLF14_01810"
     CDS             complement(108764..109426)
                     /locus_tag="PLF14_01810"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70065.1"
                     /translation="MIDIHSHILPKVDHGSSSLEESLRQLKTADKFGVSSIVCTPHFY
                     PFKESIDRFLSRRDKAFLELSAANNTDIKLYKGAEVRLTTGLGNNKKIRKLTIEDTNY
                     ILIEMPFSSWDNMLLNEIVNLQSMGLIVIIAHIERYSPIQVQKLEGLDVLAQINAYSL
                     YSPLQALRIRRYVKKGLINFIASDTHIYNYLYSYRTLKRSRFFLGSTYDKFMANASKI
                     IG"
     gene            complement(109419..110171)
                     /locus_tag="PLF14_01815"
     CDS             complement(109419..110171)
                     /locus_tag="PLF14_01815"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HOT70066.1"
                     /translation="MADIEKREFLLFDNKSFPVAEAIKAARTNLMFITAKDECPVISF
                     TSSHMGEGKSTVCANMALAFAEAGFKTLIIDADMRKPTINKYFKIKSSPGLSDILGGL
                     VKENFIYPTKYKNLHVLPSGTIPPNPSELLTSERMTLLLSELRNTLDYIFIDTPPILI
                     VTDALAISDKVTGTVLAVRQNYSRTNLIKDAVDAINKVNGKFLGFIFNDYNIEAMSYN
                     KQYRYGKNGYKYERYGGYDNKPVTPPVNLDND"
     gene            complement(110164..110871)
                     /locus_tag="PLF14_01820"
     CDS             complement(110164..110871)
                     /locus_tag="PLF14_01820"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOT70067.1"
                     /translation="MEELDIRQIFSYLKRHFWLIVSIVLVAASLSLTVTYFFITPQYE
                     ASATLYVFNNSNRSDTTISSSDITTSQKLIATYSVIMQCDNVINKVIETTGLDYTPGQ
                     IRKMFSGSSVNNTEVMKITIKSEDPVDAMIIANAFIDVAPEEILRVIKAGAVEILDRA
                     VVPSKPSSPNMVKNVAVGVVLGFIVAVAFVILYELFNTKVRTEENLLRVVKAPVIGYI
                     PDLKLYQDEDKKGHFYG"
     gene            complement(111033..112220)
                     /locus_tag="PLF14_01825"
     CDS             complement(111033..112220)
                     /locus_tag="PLF14_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70068.1"
                     /translation="MDLSTLIKYLRKNIVLIVIVVLCCAILASVFKIITNYSNYRKEE
                     ENIQVLQTELDSLVARKDKLLKNKADYEKNELYMYIKPINSSSLKKYTAVYNIEKNVA
                     LLIAVVSDESLLTEVYKESGISVPFKVVDIFVDFTATVSYNTLTMHVYAKGADDLVML
                     KGIANKCFERAISTVNSTLGTGNITLLYETENLSGNTALADIQKTYNTSYTDIQKAIS
                     EADKQIAEKQTVIDEFNYDSYNKDVFLYTLLGIVLGMFLAYVLIVARLQLSSKLIDIE
                     QIPGLNLFSVGKDINIIAEQALVPVIHACGKTTDPIGITGSENIEAVDIIAKYLKDNT
                     DYDWRLLNNFFDSVDIAKSFLDCSGIILVEKYRSSQVKKVNRILEVCKNNNITVYGVI
                     IVE"
     gene            112500..114107
                     /locus_tag="PLF14_01830"
     CDS             112500..114107
                     /locus_tag="PLF14_01830"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOT70069.1"
                     /translation="MGKKKTVKKTEKKNTKKDLIKDFFYLEQDTKKLTYFEIVTTAFL
                     YVFLSSFALIVSDKIYENITATKLYALISMFGIWFILMCVGLLEKIRSKKVNAEYFKQ
                     AVKNLSVTDIALIAYTLVAFISAILSEYREVAFIGDKARNEGFLIQLMYISMFFMLSR
                     TYVHKLELKIWALASMPVALIGILQLNGYYAGKFTYDILRHGRSFLTTLGNIDVVSTY
                     LVTPIMICTVLYCQTKEKFFRLLYIASAGLCFYLSILIGVDSGFVGMATAIFFMFPFI
                     AKDRLSASRIFHIVSLFLFLIYLEDQLSKIGTRHFVFFEKPFDDKLFLGAIAAIIVSV
                     IIYFAGKVIKTEKKFVWPIAYFVVAAAGVYFAVKKILVMPEPTGSSVKLVLYQFGQIL
                     QGNIQDHYGSNRIYAWRVIYRFFKEKSVIGHGPDTVRALWMREEYMYSTKKYGVVFDK
                     AHSDYLQVLVTTGILGLTSYLVFQFSLLIRSFKKFNQPLVIALVTACICFCAQAFFNI
                     GVPIVSPYGWILFGMLASVLRKKEETA"
     gene            114136..116064
                     /locus_tag="PLF14_01835"
     CDS             114136..116064
                     /locus_tag="PLF14_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006427182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HOT70070.1"
                     /translation="MKKDKKNISLWIKKIILVALDIISINAATILSLFVRFELSFSKI
                     DVQYIENARQFLWIDTTFTLIIFTIFGLYRGLWKYASIKELFNIIVACTISYTFKYFV
                     TNWMGISQPRSTIFFGWVLLIILVFATRFSYRFLRYLVHENAQRKDIKRIMVIGAGDA
                     GNMIIREIRSSGVLKSKVVCVIDDDENVHGLNIQGIKIIGGRDKIKEAVKKYKVDEII
                     IAMPSVPSREKKKIYDICKDTQCTLRTIPGMYQIINNEVKLSDLREVQIEDLLGRDTV
                     DINVDEIMDYVKGRVILVTGAGGSIGSELCRQIAGHNPKKLVMLDIYENSIYDIQLEI
                     AGKYPKLDMEVLIATIRDEKRIKSIMKKYKPEIVFHAAAHKHVPLMETSPNEAVKNNV
                     FGTLTLVKAADESSVDKFIMISTDKAVKPTNIMGATKRICEMIIQAYNKKSNTEFVAV
                     RFGNVLGSNGSVIPVFRKQIEEGGPVTVTHKDITRYFMTIPEAVSLVLQAGYYAKGGE
                     IFILDMGEPVKIYDLATNMIKLSGRIPDVDIKIEITGLRPGEKLYEELLMDEEGIEKT
                     QNNLIYIGRPIQFDEKIFFKNLEKLKKMADDEVSCMKLLIAKMIPTYTPEVTDTTDEN
                     EVNGCMTQLLAQDNQIKQ"
     gene            116113..118374
                     /locus_tag="PLF14_01840"
     CDS             116113..118374
                     /locus_tag="PLF14_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT70071.1"
                     /translation="MKTDDKIQKLVDSYGSWFKEDTGFSAAKMTNKHAPYPMLFSPIK
                     INSLTIKNRLVMAPMGNINMAEETGRPNEKMIEYFRERAKGGVGLITTGLIPISFGID
                     HSLIELDKLSYFPRIDRSRTVYVGWRNLAHAVHSYGARIFVQLTPGLGRVGNPQCLTN
                     QFKFPVSASVNPNWYMAEVPCMPLTDAACRKIIKNVGQAAADAKAFGLDGVYLHGHEG
                     YLLEQMTNPAFNRRKLGRYSDWQAFGVEMIQQIRKRAGKSYPIMYRIDLSLALNETYG
                     EKMNKPALKKFKNGRTVEMTLDYMKNLVSAGVDIFDVDLGCYDNWWLPHPPEGMPPAC
                     FADVARIVKKYFEENNILSNAGVKVPVVAVGKLGYPDVAEEVLMQDKADMIMLGRPLL
                     ADPEWPNKARAGLTKDIVPCIGCQEGCINEFVEGGHPQCAVNPRTAFEHLYSEQPQPA
                     EKIKNIAVIGSGPAGVMAAVTAAKRGHDVTVYEKQAVIGGRLNAGGKPKIKFDIQNYH
                     EYLKNLLDTTSKEYKLKVILNTEANAELLKKEGYDTVIYAAGTKDVELKLPGYEKADK
                     VQAVDVLEKPELVKAAKKVVVIGGGVVGCETAFFLNKELGKEVTVIEMEPYFMNHVCT
                     ANRTYLIYYLEKAGVKLFNCTKLVSYEIGGIKVSRNMSKTVPDPYNTWSPILPPNIEN
                     PLAKPIKEELEEQFIEADIVVQAAGGRCDDTGFYALQKQLPKCEMYNIGDSFKGGKVF
                     DATKAGYAVGLTV"
     gene            118398..119678
                     /locus_tag="PLF14_01845"
     CDS             118398..119678
                     /locus_tag="PLF14_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X"
                     /protein_id="HOT70072.1"
                     /translation="MFLTDEEKDILNGSKGEVMAKVAKSVVMYGEVFDAKRLVPVTAP
                     SHLVTSFGISLMKAVFAIMDEINDAGVTTIGKFTVDPRPLDYENVKCNLLEKLVFGII
                     YGKQKDYEEQLKKTGLKDSNAFSCTCYMEEVGNIPKKGDILSWAESSAVVYANSVIGA
                     RCNRNSGIIEMFGAILGKVPEFDLLTDEGRKATWIVQIKTTKKPEAQVLGSAIGMKVM
                     SEVPYIKGLDKFIGKELNAQTKDYLKDMGAATASNGAVGLYHVENLTPEAVELKDALI
                     KDKAKVYVIDDAELERVKKNYPVIWKNADAKPSRCFIGCPHLSYNQLIEWTDRIGKEL
                     EKQGKKKVSLETVFTAAPDVVAHFKENKEYYDRFISYGTKISSICPLMYMNNPICGKR
                     AVVTNSNKLRTYTTARYYTDEEIAGIAVRGDIAR"
     gene            119675..120121
                     /locus_tag="PLF14_01850"
     CDS             119675..120121
                     /locus_tag="PLF14_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HOT70073.1"
                     /translation="MKKEFKGRNVVAGTVQTTAVVSKNGMNTLATFQKSLLANKKTVL
                     GSDQNNPDLYKKEITGKAICLPKTIGSTTGGMVLQSAAAMGVAPKAMLFSESIDSIAA
                     AGVILADVWTENSIVTVDKLGQDFLDYVKDGMTVTVKQDGTVIVEQ"
BASE COUNT        35402 a        24912 c        22906 g        37009 t
ORIGIN      
        1 ggtctttatg ggtgctctgt atagtttctt aatggagtat ttcattatta cctcatttcc
       61 atcagttatt tcacattagg tatgtagtaa aagtcataat ccgtatatac gcaattccga
      121 ccttctttct ttgttgcact atcggaaaaa tattcgttca taagaccata tattgcggac
      181 acatcggctt cctcttcata atatatgctg agcgatttaa tcattttatt caatttagcc
      241 tcatcatact caaacagttc accataggta tacattgaaa catatatggt atcttgggta
      301 tctgcctgat agaaaccgat aaccttgaaa ttgcgactga gatgataggg ctctaaatta
      361 atcagcatta tgtctatatc atctaccgta tatcctttgg caatattttc tttcatccat
      421 gcaggcaaga ttacatatcc ctttttttcc tcctcattct tcttcatcat atatgaagag
      481 tcataacctt taatgaaagt taccttacaa ttatcttttc tctctatagt tttgatgctg
      541 gttataccct gataatatga aagcctgtca atcggcccta tatgccacga ctttttatat
      601 gcaagagaaa tatcatcacg ctgcttcgat aagactgtct gagcctctat atcctccgca
      661 tttgcaagaa agtcttcctg cctgttgatc atgcttcgca catactcata cggcatattc
      721 aaatcgattt ctgcatcttg tcctatcctg cgtacaggta tttcatgcaa tgaaggagct
      781 tcctctttat ttcccggcaa caaaaacaga aacactgccg ctacaattat aaggatagcg
      841 acaattatga atactgctat tcttgctttg actttggttt tttctttttc cattatctca
      901 cctctcggtt taataaaata tatacacgga cttaaagcct tatattgctt ctgttaatat
      961 tttgattgtt tagatatgtt tgatactatc ataacaggca tttatgtcaa cgctttgcag
     1021 gaaaagttca ttaaaagttc atttttacta ataatttagt tacaataagc actaaaaaat
     1081 cgagttttac cgatatttac tttcccgata atatattctc aaatacatca ttcttctcca
     1141 catccggtcc gactaaactt ctgtacaatc cgaaccatct gcagtcgtac tgactttcac
     1201 cctgtcttac ggcaaaggac catatgtgct gactcttatg acagtcatag ccttgtacgg
     1261 ccatttcaaa tgctgtaagc ccgttgaatc gctccagcgg aatatcccag ttcattaaaa
     1321 tcttgttttc ctcatataaa tgaagataca gtttcggagt atcccataca ccgtattttt
     1381 catacgatga catatatttt gtgctgtccg cggcatattc caccgctaaa gtaagggcat
     1441 atgcattcaa catatgagct ccgtgaccgt actcgccttt taagtcatgt cctatcacca
     1501 cttccggttt gaatcttctt aaaagttcca cctggaaatt cagcacatcc tccagaggat
     1561 atacgctttt agcatgttcc agatttgaac aatatttatc ctcaaattca cttataaccg
     1621 ggtatgcggt catgcctacg gtccataatc cgttcagcaa ctcatgaggc ctgtacggtt
     1681 ctccccaatg gtttgtaaga tatgcaatct ggactttgta ttgcaactgt ccggcataat
     1741 acggcatcat acctccgaag tacagatgtt catcatcggc atgagtgggt aacagcagca
     1801 tatcggcatc ttcatacggg ggattccata tctgtaccca tccgggaaga ttgccgtatg
     1861 tgaaaacata gacatcacat attgttgcac catcgtttgt caactcaata cttattcttg
     1921 tgcaaccgca cggcaactct atatattcat gtataaatcc gttctttccg cacgtcaatt
     1981 tttcatcacc tatattaagc gtccattctc ccggtatttt gtcccatatt atgtataaag
     2041 agtgcatctt ttcataagac gatatctcta tttttgatcc tttgttaagt ttgtgtttgg
     2101 agtagaagct gtaatcggaa agcttgtcgg aataatgccc gtcaacactt acatacacct
     2161 tcttggttat atcctccgca tcgggtttta tctcttctgt aggctcggga gtcggctcgg
     2221 gtgttgcaga cggtgtttcc gtttccggta taactgtggg tgtcggtgtc ggtgtgggtg
     2281 atggtaaaac tactgttgtc ccgctcggag aaataaccgg ttcggcacaa ccgcacagca
     2341 caagcaacag agctgcaact ataatcaata taagcaattt atatgctttc cttttcataa
     2401 gacaactaac ctttatataa caataccttt cttttctttg ccgcatatcc gcttaacggc
     2461 accaatatta tattggaaat tcctctcttt gtgaatataa tatatggagg gtaagtaagg
     2521 agacttgtta tgacaaacaa actcggtgct gcaaaaagag cggtttcagt aatattgaaa
     2581 actctggctg ttttctttac cactttagct ttaatagttg tggctctgct gggagttata
     2641 tatgtattgg taaaaggccc ttctcctacc gcaaaaaaac tgtttgtgct gtcggtaaga
     2701 gaaacaagtg ctgtaggctt tctggccgac atatatctgt cggatgaaga gcttgcaacc
     2761 cttactgttt cggaaattgt agaagaaccg aaagaaaaaa ccgatacctc cttaatcgaa
     2821 atacccacac ctacctcttc attacctcct tcgggggagg atgctgatat tacggatacc
     2881 gttccggagg aaaaagatat agaggttgtc agaataaagg gaagccttta tgaagcggtt
     2941 atgcttatag taaaagatcc cttaagagta tttgtgggaa ctcccgacga attgggagga
     3001 aaaggcttaa agcttacgga aatggtggaa aagtatgatg ccgttgccgg tatcaatgcg
     3061 ggcggtttct atgatccctt gggaggcggt catgaaggca ctcccgacgg tatggttata
     3121 tgtaacggtg aagtagtctg gggtgacccc gatgtaaaac tgaatgtctc cgcctttgat
     3181 gataagggta tattgcatgt aggcatgatg accacacagg aggcaatgga tgccaagata
     3241 cagtgggcgg tcagtttcgg accccctttg attataaacg gaaaagtgca gagttttgaa
     3301 aaagacctca taagcggatt aaatccccga acggcaatcg gacaacgtgc cgacggagcg
     3361 gttctgatgc tggtggttga cggcagacgc atgaactccc tgggcgccac atatgccgac
     3421 ttagcggata ttatgctgga tttcggagca gtcaacgctt ccaatttaga cggcggctcc
     3481 tcaacactta tgatttatga gggtgaggta ctcaatgtct gtgcatcggt aaccggtccg
     3541 agggctcttc ccacttcatt tttggtaagg tgattatgag agataaagca gttatgcata
     3601 ataaagacaa tgcgggaaaa cccaagagta aattcgtaaa aggcttgata atatattcaa
     3661 tatgctttct tgttgcggtg tttatcggtt tggttttctt ctggcaatat ataaagtcct
     3721 atgaaatatc ccgaccggaa aactatatga acgagtttat ctctaccgtc aatgacgata
     3781 ccgtaaggac cctgatgact gaaaacaaga agattgaagt atcggaattt gaagaggaaa
     3841 acacagtcat taatcaatta tacttttcac ttcttaagaa tgaagagtat aaataccgta
     3901 agatgccggg gcaatatgcc gacacatcac cggtctacat aatctatacc gactcggcag
     3961 atttaggtaa agttgtgctg acaagtaagg gtgataattc cgcaaaatac ggtttcaatc
     4021 tttggcagac cggcagcttt accgttttgg attgggtact tttacctgag caaaaaacat
     4081 taagtataac ggtaccggag tatgccgatg tgtatgtcaa taatataaaa gtgggtgaat
     4141 catatatcac ggacaatgcc gtagcatata caggttttga atatgtcaat cagccttttg
     4201 ataaaagacc tacaggaaag aaatatgaga taccgggtat atacctggag gcggaagtaa
     4261 cggctgaaga cgacaagggg ttgcctttag aacttaacaa aacggaaaac gagtatgcct
     4321 accctttagt tataaaagaa acttattcat ataaagtcac cgctccgaaa gaggttaagg
     4381 tttatataaa cggagtatta cttacggaaa agtatattga aaacggtgaa gcatattatc
     4441 cggggcagga aaatataacg acatactcgc aggttccttt gttaaacaca tactcagccg
     4501 acaatttgta ttataaaccg gatattgaat gttatgatgt aaacggaaag tctttggagg
     4561 taacgaaggc ctccgatagc aagaatatat tctatcacta ccccgcagac gagcaactga
     4621 aaaacactca cgaacagcat gctctgaatt ttgccaaaac atatattaat tttaccgtca
     4681 atgccacagg cgatatatgg ggatatttcc ccacattaaa cgcatatctt gttgaaggca
     4741 gtgttttaca gtccaggctt aagtcggcat gtgcaagtct gtactgggta atgggctcca
     4801 ctttggagta taagtatctt aaagttattg atttccgtcc ggtaagtgaa acatgtttca
     4861 catgcactat gtctttcagt gcaatacata aaacatacta tgaaacgcga tatctggaag
     4921 attcgtttga cctggtgttt atactgtata ataataagtg gaaagtagcg gagatgcttt
     4981 cggaataaat aaggagtata atcatgacat ttaaagataa cggtcttgga ataaccgcag
     5041 ttataccgtc attaaatccc tcacagaaac ttcttacgac ggtttcggga ttaattgaac
     5101 aaggcttttc cgatattata gtggtaaatg acggaagcag tccggagtat gacggtatat
     5161 tcgatgaagt atcttctacg gaacagtgca ctctattaaa gcatgaagtg aataaaggaa
     5221 aaggagctgc tcttaaaaca gccttttcat atattcagca acggaacaaa gatattaaag
     5281 gtgtagtcac agcggatgcg gacggacagc atcttcctga agacatattg aaatgttcgc
     5341 agcacatgac cgatgacaaa acggttatat tgggagcaag gaatttccat caaccggatg
     5401 ttcccccgaa aagctttttg ggaaacaaga taacttcctt tgttttcaaa acgggatgcg
     5461 gaataaatat aaacgacaca caaaccggac tgagagtatt tcccgtaaaa cttattcctt
     5521 tccttctttc agttgagggt gatcgttttg aatatgaaac caatatgctt ttagagatga
     5581 aacccgaagg tattccgttt aaagaagtgg aaataaccac cgtatatgag gataaaaatg
     5641 tagggtcgca tttcaaggcg gttcgagatg ctttcaggat atacaagctc atattcaagt
     5701 ttatgctcag ttcaataata tctttccttt tggacaacat tgttttctat gtgtctttgt
     5761 tgatatttgc aaattatgta gagaatacaa ggattcttat aagtacggta atagccagag
     5821 cattatcctc attttttaac ttcaatttca ataaaagttc ggttttcaaa cataagtgtg
     5881 attataaaaa gtgtcttatc aaatactatg tattatccgt atccgttatg cttgcatcgg
     5941 caggtcttgt aactttgctt tccttgatta tccctgccga aaaagaaata ataatcacat
     6001 tgattaaaat aggggtggat gcaatattat tctttgtttc ttttcatgtt caaagagaat
     6061 gggtattcaa gaagtgtaaa tagttattta atcgttttgc tcctcatcta tgtcattaaa
     6121 ccaaaaatcc atgtattatc cgtatccgtt atgcttgtat tggcaggtct tgtaacttta
     6181 ctttccttga ttatccctgc cgaaagagaa ataataatca tattgattaa aacaggggcg
     6241 tgtatacaat acttttcttt gtttctttcc atgttcaaag agaatgggtt ttcaagagtt
     6301 aatatgtaaa gggtggtttg taccaccaaa tacatatggc tgcggctcat ttgtggtgaa
     6361 ttggaaagca gttgtaagaa atgagtattg tctttttctt ttatttcaac aatccctcaa
     6421 cataggactc ttcaaccgcc atgtcaaagt aatccagagt attttcatct ccccaccgga
     6481 gatttcgcat aatgcctatt cctggtatga taactgttgt tgccgacttt ggaagttcct
     6541 gtcggataag ttcacaaatc cgcagcaagt gtgccttggg cagaccgcat cggtcatgtg
     6601 ctgtgtaaat atgattaatg ggaaactctg caagcccggc aagtttttta caatgttcat
     6661 ccagcgggac cgtaggcgtt agaccgtaga gcaatcgtct tgaacaggaa tccccgctga
     6721 acaaaaaacc agactgcttg tcccaaaaca gaacgctccc cagtgtatgc ccgggcatat
     6781 ggataacctc aagaattcgc tcacccagat caatcttctg tctgtccttc agcggattca
     6841 gtacgacatt atgtatggga aaacatattc ccggaatccc tttcaggacc agatcataat
     6901 catcaggatg aagccatgct tcaccaaccg actcaagatt gtaaacatga tccgggtgcc
     6961 catgggttaa cagtacctgt gtcggaaggc ctgtaagctg acaagcatag gaaaacaaat
     7021 ccggtgtatc tactgccaaa tcaattaaag cagccttttc ctgtccttgt atcaaatatg
     7081 aattctgtgt tgccctgccc ggtgcgtctg aaaatccgtc agaattcggt ccgtgaagac
     7141 tgtcggaaat ccgatatatc cctcttgaca gttccgtgta aaacaaatta cattccaatt
     7201 acatcgtctc cttttataaa taatccttat gagtaatctt caattctcca ttcatccagc
     7261 cagtctatga agatcattcc attcgggtgc tgcggagccg gttcctcaaa cctcagcgga
     7321 agccagacat agtctgcgac ggaagtgtcc gtatcattct gcacaagaat actccggtaa
     7381 ccttgtagcc cgtatttcct gatcagagat tccctgaaat gctcgccttc gctgagcggg
     7441 actcggcccg aaaagaagcc ctcaaaaatt tcctcgcaga aggctgccgg tatttccatc
     7501 gcattcggca tccagcggtc tgccaacgct acatacagat tcttctttcc aggcatcttg
     7561 aacaccgagg aaatctgcga gtgaaaggat gtgccggtcg gatcgccggg atgtggatct
     7621 ccgagaacag taaaagggcc atgccagctt tccgacactg caacttcgct gcggtttgga
     7681 aaatacccgg tcgttccgga tgtaataaga tagtgcagtc catttcgctt aaagtgtgcc
     7741 ggcgcttccc gaacaaaagg cggatacgga tgcgggaaat gatgggagca gacatctgtc
     7801 acatctgtgt aatctgctgt caactctgcg caaatcagct ctgaatggac tcgttcaaag
     7861 taataataag cttttccgtt ctcatcatcg acaagatcaa aatccccggc attcatgcca
     7921 agcggagata ccccgctctg tacaatcgaa tacggtccta aaaagctgtc tgcgaccgct
     7981 atcgtaaccg tctgtcgcct gttgtcatct ctgtgcatga tcttcatcca gcagacatat
     8041 ttctccgtct tttggttaaa caggatatgt ggccgatcca aacatgccgt ccggggattg
     8101 aacggtgata acggatcgtc aagcacaggc tcaacgatca atcccttatc ctcccagttg
     8161 tagaagtccc ttgacgcata ggcacgaact ccccaggtcc agataccact accgggaaca
     8221 gagaattcct tgttttctcc atacagataa tatgtctcgg tttcagcatc gaaaaagata
     8281 ctccccccgt gggcctgaat gcgctttcct tccgtgtcga ggaaaggctt tccgggtcgg
     8341 atactatgat ttttcatatt attcgaatct ccatgtacca ctcccaacct cctttgtttc
     8401 tccgttccat ttgatcactg cacgggtatt gggcggaact gttaccgtca gaacggagtc
     8461 tgtatattcg acagaaataa ggccggcctc gcagataaac tcccgtttca gccggtccag
     8521 accaatcaca cggtccgggc tgacctcaaa tctggcaaaa cccggctcaa gcggtctgac
     8581 tcccgcaaga acggaaaaga aatattcatc cacaataccg aaagcataat ggtcaaagct
     8641 gatcttttgc ggtgttccgt caggtgctac ggcactccag ctttcccata ttgttgtagc
     8701 tcccatgctg acttcataga gccagcccgg gcagttcggg ttcatcagga ccttcaatgc
     8761 cagatcgtgg cggccgatgc tgtccagtac tttcagaatg tagggcgttg caagaaaacc
     8821 ggttcctata cgataatcgt ttgaatgaat caattcaatg agtttcccgg cgtattcctt
     8881 ttccaggtct ccgtcaacca attcaaatgc cagggcaagg atgtatcgtc ccatatagtc
     8941 tgcatattcg tgttgtagag gaagcacttc tgcctcgatt gctgctttca tttttcgggc
     9001 gatacttccg taatacgcag cgctcttttc gtcgccaagc ttttcacaga tttctgccag
     9061 caggtttacc gaacggaatg cgtaaaaagg agcgatatac caggccgttt ccttacaagc
     9121 ttcaaatccg ggcttttcct tccccggaac aagccattct ccgaaatgga agccggtatt
     9181 ccagaggtac cgatcgtttt catcaagtga agcgccgcct ctgcgcatcg ccgctgtgcg
     9241 gattagctga ttagcgtatt tcttcatagc gggcagacat ttcttcagaa tcaggtcatt
     9301 gcctgtcagc ctgtacatac tgtacggaag ttctataatc acatcgctcc acccggcaac
     9361 cgaaggcagt tctcctcctg ctgcggattc gccttccgcg ggcagaatat cctccagccc
     9421 cttggccggc tttgacttat catactcgcg gctaatcttt ctaagcatga agtcatacat
     9481 ttttgtcgag gggcaggtaa tcggtaccgc cccgctttcc tgctgatctg caaggaggct
     9541 ggaaagccag ctttcataga aaggtgtcat ttcttcgttc agcatgcttg cggcattata
     9601 gaccagaaca tcacctgtcc atcctgcttt ttcgcgctgc gggcagtctg tcgggattga
     9661 catcatattg tttctctggg aataccgaat gttttgatac agccggttca gcttcggatt
     9721 gctgcaattg aatccgcctt tgttctcctt ttccgtggaa agaaggacgg cggtcacatc
     9781 ttcagggcgg atatttcttg cgccttcgat catgatgtag cgaaagccgt gaaaggtaaa
     9841 gattggttca tagagagtct tttccccgct gcagattacg gtttcgcgct ggcgggaaga
     9901 tagcgccgcg aaataattgc catctttatc cagaacttcc gtatgatgga tgcgcacttc
     9961 cgttccgcgt tcttcccgta gcaagactcg gcatctgccg gcaattacct gaccgaaatc
    10021 ataaataagt tcgccctttg ggctttgaat gaccttctta actgagaaga tttcaacagc
    10081 cctaaccgga tcaagcgcct gagcccggag aatatcgaag ggataatcac agagcaccgg
    10141 tctcaagggc tccgaataag gctttgtcag atcgattttt tctccgacga acagatccga
    10201 atacaggata tttgtctggc gacactcctc actgccgtcg ctgaaaacag tctcgcgggt
    10261 tccatctgca tattcaagtt ccaattgata caggattgcg ggcttttcgt gatcgcagcc
    10321 gacaggtacg gtctggctgt tgaagtacca gccgtctccc accagcatct ccagcttgtt
    10381 ttttcccgta tgcaacagcc cggacacatc gtatctttgg taatacaaaa gcttgctgta
    10441 agcggtgaac tccggagcaa attccctgtc gtccgctctt tctccgttga ttatcagatg
    10501 ataaacgcca taacaggtgg catacatgac ggctgagcga agtttcccgc ggatttcaaa
    10561 acctctcgta aaagagagca ccggattttc gataccgtca gagagcatct gatgcggatt
    10621 tctcataaag gtggattcaa cccagcatgc tttccagtct gcaaggtcaa gacctgtata
    10681 aaaacgtccg gatgcaccgg aggaatcacc gtcagcggta cacacggtca cagtataccg
    10741 atattcagat tttgagggaa gcttcggtcc gtcatatgcg acaaaactct gccgcctgct
    10801 ctccactgta ccgctgtccc acatcgttcc tttctcgcct tccacaagga ttcggtaaga
    10861 acgctgcaca atgttcttgt gttcagaact gatgcgccag gaaaactcaa tatccccgtc
    10921 aacgcaggga tttgttctat gatttacgcg taaatgatac agtttcatgt ctctgttcct
    10981 tccaaaatgt aattgctctt ttaatccatc cttcggcttc tgttttgccg cctctgccaa
    11041 aaccatgccc gccgtgttca ggtttttcta taacacaggg gacttctgct gttgtgagag
    11101 cacgagcaaa agctgagctg agttcacaat ccaccgtctc gtcatctctc gcgtgaacaa
    11161 taaaacacgg cggatagtcg cagtcaatat tctgatcaac gttataaggt ccacaataag
    11221 attcatcgac tccagatccg agaagacctt taagcataag cttgcgatac ggttccggca
    11281 actgttccaa ggctttccaa acattaagca ggggataagc aaggagcact gcttctggcg
    11341 caggaaggtt gaatttccga tatcccagtt ccttaatgcc cccgcaggca gcaagatgtc
    11401 cccccgcgga aaagcccccg actgcgtaat gcttcatgct aacatcaaga ttctgttcat
    11461 tgctgaaaag gaaacgaatc gcggctgcca gatcctccat cggctttggg aaaagcggtg
    11521 ccaaagcccc gacccgatat gtcagacaaa ggcagtctat tcctagcgct gcagcttctg
    11581 ccgctgcggg aaatgcctcg ggcagattgc aaactgcacc gtaagcgcca cccgaggcga
    11641 gtatcagcgt tcttcgtacg cagtcttttt cgtagttgca tgcggtttct gccggaaaat
    11701 ggaccagttt aacattcgct tttccggggc acgcagcagt ctcatcagac ctgtatacat
    11761 catataaaac ccttcctctt gcagcattac ggcacagtgt gttcagaccg aaaaccatat
    11821 cgtccgcatt ccacgccgga tctgcagcct gaatatcttt cagggttctg ctttcgtcag
    11881 agaatctgcc ggctcccctt tctgaaaggt catagatcaa ctggctgaac agattgtcct
    11941 gcagtatgct gcggatttct tttattgttg tttccggtgt aatcatagtt ctcagtcccc
    12001 ttcaaaaacc agttcttcga ttctcttgct gatataggac cctgaccctt ccaaatcggc
    12061 gcggttcgtc ataaatacaa cgcccagcct gtcaataggg ctgatgaaga agtgcgtgcc
    12121 aaaagccccg ctccagccgt aggttccggc tgtcgcagga ctgccgccct tttcaggatc
    12181 ctgacgaatc ttcattccca gtccccatac aaagccgggt tcgggctcaa gatggacgag
    12241 aggcgcttca cttcgcatca ttcgaaccgt ttccggctgc agaatccgtc tgcctttata
    12301 ggaaccttca cagagcagca tctgtgcaaa atggctataa tcatcaagag tggaatacat
    12361 tccgccacag ccatgttcaa aagagatggc cttctggtgc atggctcctg ccacatcatt
    12421 ctttgttccg gtgacatctt tcaggctgcc cgcatgcctt ttatatacct tgacaagcct
    12481 tttctgtctc tcttctgata ggaaaaagcc tgtatcaaac atttcgagag gatcgcagat
    12541 ttctgtgcga acaaaacctt caagccgtgt gccactcaca cattcaatga gatatccaag
    12601 cacatcaaaa ccggcgagag gtgaataacc cgttccggtt cccggatcaa aatccagggg
    12661 ttgagcagca aaatacgaaa cttcttccct gagcgatccg aactgcggaa gacctttcag
    12721 aaggctcagc agactgaccg ttccctgctg cattcccgag gaatggctta agagatcgcg
    12781 gactgtgatg tcgcgggagg caggttttgt tttcacttta ctgatattga aaaacggaag
    12841 aagcagaggt attcttttaa gtaagtccgg tccgttggca tatttcggat catgattcac
    12901 ctgcatatcc gcgaattccg tcagataatg cgaaacagga tcatctatgt cgataagtcc
    12961 cctctctgca cagatcatca ctgcggcagc tacgatcggc tttgtcatcg acatgagtct
    13021 gaaaatactg tcatattcca cggatctcgt tgcttcaata tcgcttaatc cccattttcc
    13081 gcggaaaatc gtttcagctt ctgaaaacac atgcaacgct ccaccggcta gttcatgtga
    13141 atcaatatag ccttgcatca cctgattgat tgtttcagtc ttcattgtta tgcctttcgt
    13201 cccggtaagg actctggctg ctttcctcca gcgtctcccc attctttctg tccactctga
    13261 cttttcgtat ctcttccgga gcctttccct cttctaccca cttcatcaga gcaagcatgc
    13321 catctttttc ggtaattccg ggtccgttgc cgaagcaatt accatggttg tcaccggggg
    13381 ttatgtatag tctcataaag cggtccaacg cactctgcga accaaaatgc gtgagaagct
    13441 tcccgtaata gtccagcgtc cctgccaccg gtatcagcgg gtcgtccata ccgtggtcga
    13501 ttatgagttt gccgccggca ttcacaaatg catcgattgc aggattatca ccaaggttat
    13561 ccgaaaactt tctgacgcct tcttcaaaaa gctcggtgaa ttccctaatc ttcatgccat
    13621 caaacttctg acccggtgtt tccgtgatcc atctggcgca gattggagcg agaaaaaaag
    13681 gctttacgtg ccgcgaccat ggcatcggat aataataggt gccgatagga atcacacact
    13741 gccaattctt cacgccgggt gcaaaaccgt accaaaggaa actgccgtca gcctttctcg
    13801 ggccacggca aagttccgtc atgaatcttg catcggcttc tgtaatggaa tcgctaccaa
    13861 tatttttccc cacacaggaa taaaaatcaa attcagggat cttttccaag tgataatact
    13921 tgtccgcacc gccgtttgct tcatgaacgg cgtcaatgaa aaactgattc tttttagctg
    13981 aaaatgtgat tccatactcg ttcatgagtg tctgagccca aaaaccggag accatcaact
    14041 tctgccagtt gattgcgggg caggatgccc agatgccgtt atagtctttc ggataattct
    14101 ggacttccat gagcgattgt cttccgcctc cggaaccgcc gttcatatag gaccatctga
    14161 cagggcggtc atggagaagc tctgctatag cttttccgaa aacagtcata ttatgagtgc
    14221 tccgaagtct ccagttttcg tacaactcac ggttgagcgt tccggttttc gggtcgatgg
    14281 tgtaatcgtg gagcccgccg tcattgtttc ccgcgaacat cgtcgctgca gtaaaaccgt
    14341 tcatgacggc aaaggggacc gtcaaaccac gctgggcgtt atccggcttt gtcagataac
    14401 ttcgtcctcc gattccggtc cctcctccgg cggttcccgc aaaacgatcg ttccaggctg
    14461 caggcgacca aacaatgagc gtctcagtat ggttaccggt cagatgctcc agttccactt
    14521 cataatatgc gggtaggcct tcaatctttt ttccgccgcc gaacatacgc tgaatcagag
    14581 aaacctttgg atgatactcc ccgctcccgt taaaactgac acatatattt ctgaacacct
    14641 caccgggaaa cagccgtgca aggccggctt cgacatactg acccgttgcc ctccgttcga
    14701 ttccctgcgt cggagagaca gaagtcatat caaagaatct gacgttgtct ctttcaagca
    14761 tgatacagtc tcctgttttt cagtatttcc gcgacaatct ggtctcgcat tcctcgcggc
    14821 taatctcacc ggcattacat tgagccataa gttttgcgtc gtcatccttc agcttataga
    14881 atgccataac cacaacgctc agcagtgtgc caagtgcggg aatcagcata aagacaatcc
    14941 aaagagtgtc cagtgcactc tgggtctgca ctacattgag ctcggcaagc tcctcaaaac
    15001 tgtctgcctc aacagtaatc cattcggaaa gccccaggat gaacacgccc aaagaagaag
    15061 cgatgcttgt cgttaacttg ttaacgaacg cattgaggga gaagaaaatc cccgagcagt
    15121 cttttccggt cttatagcgc gtatactcaa tcgtatccgg agtgaaaaaa gtctttgcga
    15181 tattggcaac attgcccggc gcagcctgca gcaccagcag gattgtaata acgacaaaat
    15241 tgttgtaacc ggcaaagaaa atagaaccgt agattagagc tgcggtcaac ccgcagacca
    15301 tataaacctt tttctttccg aatttgcggc ataacttatc ggtattgagc tgggcaatca
    15361 gctgagggat aaaggcgatc gcgattggaa tgataagaat catcgagtca ccgtacagat
    15421 agtatgacgc ataggttccg agagaactgc ccgtcgccag agcccccata ataagcgtgc
    15481 tgagaaggat gacagccatg tatttgtttg tctttacgca tttaatcata tcacggaaat
    15541 tgtaacgctc cgtgctcttt tcgtcctcgg tgtttttcag ttcggtattg tgttccttca
    15601 cttttgtcgc cagcgggagc atcatgaaga agaaaatgac agacgaaaca atcgcaacca
    15661 ttcttatgga gattcctaca tgttcgctaa taaggaatgt ccagatgatg ccggctaaaa
    15721 ccgtcgcaat gccgccgatg atgcctttcg tctgaaggat tgagttacgc tcctcggtgt
    15781 tgtccgtcag cgacacaatc attgagttca tggggacctc aacaagcgta tagctgatat
    15841 catacagaag ataggtcacc ataaaccaac cgatcttcac ggcgtttggc acgccctgcg
    15901 gcataagaaa gaaaaggacc gtaaaaatcg ggaacaggaa gaaggtcgcg cggtaccaag
    15961 gtaggtattt gccttcaccg gctatttttc caaaaagtcg gctctttttg atattgagtt
    16021 tgtcgatcaa gaaaccgaaa attacatcgt cgagcgcatc gatgaccttg acaaccagga
    16081 ttgccgcgga aatcgaaatc aggttgattc cctgaaacaa cagaaagagc gtcataaacg
    16141 ccgtgacgat atagccctcc aatgtgcgcc ccaaatcgcc aacatagtag ttgattcttt
    16201 cgctttttgt tgtaagccat aaagacttat cgatctttgg tttgctttcc ttgcttttca
    16261 tagaaacctc ccgatattat attatgcttg acaagctccc agccaactga tacatttgtt
    16321 tcatggtgca agtgtatcaa tcctttttcc tgaaatcaag agaaaggatt gcttcgtcaa
    16381 aaaaagaaac aaaacgggga atttgtatca ctatgaaagc agtaacatca cacgaatttg
    16441 tacttgaaag ccttaccagt gccttgattg gcctcatgaa aactaagtca tttgacaaaa
    16501 tcaccgttac ggagctttgt gaaaaagcct gtgtcggcag agtttctttt tatagaaact
    16561 acaactccaa acaggatatt cttgttaagt atctcgccgc ctgtaccaat gaatggtggc
    16621 aggagttttc ccaaaagccg gaaaaggaat tctatgaaaa cttctggccg tcactgttgg
    16681 atgaatttaa aaaacagaag gaactgctgc tccttcttaa tcagaataac ttgaattatt
    16741 tattacgtga tcacatattc tattgctgtg gcccctcacc agatgaagat gacaatgtgg
    16801 cctatatacg ggctcttctc gcaggtatga tctccggact tatagaagaa tgggtgcatc
    16861 gcggcatgaa tgatctgccg gaatcattaa acatccattc aatgctgaag gaagcgttga
    16921 gatcacatga atgaaaccat tggagtcaga ttgaaccgaa atactcaact gcatacttct
    16981 tgcgttttaa tgtaaggggt aaaaaatccc cttgacaaac cgtatctcaa gaagtgtaaa
    17041 tagttattta atcgtttttc tcatcattcc actgctcatc atctacatca ttaaaccaga
    17101 aatccatact gctttcactt gctgataata agtcttcaaa tactttgctc tcatcctttt
    17161 gattctgcgg tctttgttta cacatttttt aaatccttct acttatgata actctcattg
    17221 tttgctatct tgaatgctct gaatagctgt tctaacaata ttactctcat caaccggtga
    17281 ggaaaagtca tgtcggaaaa ggacagaagc atatccgctc ttttctttac actgtccgaa
    17341 agaccgagac ttcctcctat cacgaaatca ataacggggt ttgataaatc gaatacagat
    17401 atgactttct cggaaaaagc ttcactgtca aatttcatgc cttctatcgc aagacatatc
    17461 acatagtctt tgggttggat ttttgacagt attcttgctc cttctttttc catggcatcg
    17521 ggaatatcgt ctttttcatc acttacttcc gttatcttca catcacaaaa ccttgacagt
    17581 cttttaacat actcgtcttg tgcttcaatt agatattttt cttttaattt tcccacacag
    17641 attatgttta tcttcataat aaaaacactc tgttattgcc ttcccttacc gctacaacca
    17701 gatttatgtc tttgccgatt acataaccgt tctcttctat gcacttcctt acggtatcat
    17761 atgccagctg aggcacattg ttctgtgcac tcatatgtcc gagcacaacg gtctttagcc
    17821 cgaactctat gaacttaaca actatatctc cgcagtcctt gttacataaa tgcccttttt
    17881 ctccctgtat tctttttttc aacttcagcg gatagcgacc gtttatgagc atatccaaat
    17941 catgattgga ctccaataac agcaggtcgc tgcctttgat gttttccgct atggtttcag
    18001 taagatgccc catatccgtc actgatgtga ttttctttga gtctaaaaag aaactgaatc
    18061 ctaccggatc ggcagtatca tgcggtatgg aaaaaggcat tatctctata tctttaataa
    18121 caaatctctt tcccgtttca aatgttttct tgttttcctc tcttattctt ccgaatattc
    18181 tcggcatttc tttccacgta gaggtatttg catatatcgg tatgttatat cctctctcca
    18241 taacgcctat gccggatata tggtcgtagt gttcatgcgt tattaatatt gcatccaatt
    18301 ccgatgcatc ttcaccgata tccgctatag cctgagtaat ctttctttta gacaaccccg
    18361 catctatcag tatcgatgta tccctatatt taagataaat agcgttcccg ctgcttccgc
    18421 tgaataaagg acaaaatttt atcattatat cccctttagc tgacatgcag tcaatatacc
    18481 tacaaccgcc agtccgtttt ttatcttgcc ttccataacc atttttttag catcggatag
    18541 cggttctttc catactatca accgctcttc ctgattccat ttcatttgtg tccgggtaag
    18601 cccttccgct aaaaatatat gcacaacttc atcggaaaaa cccggtgatg atgctaccgt
    18661 aatcatatga gtcattttat cggctacata accggtttct tcttccagtt ctcttacagc
    18721 acactgcaaa ggctcttcgc cctcttcttt ttttcctgca ggtaattcaa tgagaatatc
    18781 gtcaaaagca atcctgtact gcttctccag tatgatgttc atatcttcgt ccagcgccac
    18841 tatggcacag gcaccgctgt gccgaatcac atcccggtac acttcctttc cgtcgggaag
    18901 aagcgcggtt actttttcca cctccagaat tctcccttta tacataactt ttctatccgt
    18961 tattttctct tttatatcca caatctttac cttcaccgat atattatacc atctaaaaga
    19021 ttctataaat gattgtgtat ataaaatagt ttggttagta tttgcattta tatgcctgta
    19081 taggttaaaa tctatttagg tagaggttaa tatgacaggt gtatttattt gtttggcaat
    19141 ttttttcata acgttgtgcg tttatctgta tcacattata ttcaaagaaa atcctcgtga
    19201 gaagataacc aagacggctc cgagcattat gttttttgct acagccgttg tgtcggtcat
    19261 ccttaccggc aattacactg caaccgcgct gtttttatgc gcaggtcttt tcttttccat
    19321 attcggtgac ttcttcctta tcagtgaggt aaagataaag aacagctttc ttataggtgt
    19381 ggcattcttc tttattgcgc aaatctgtta tatagtctct ttttccatat tggttccgat
    19441 tacctggttg gatattctgc tttttatagg gcttgagggt tctatcctgc tcgtttacag
    19501 gtttatgggt tatgatgcca aggagcttaa gatacctatg ttgctgtaca gcagtgctat
    19561 atcttttatg acggtaaaag ccgtttcact ggcatttgca aaacctttcg gtttttatca
    19621 cggtatcacc gttgtgctgg gtgcggtact tttcctgttt tccgactggc tgatagcacg
    19681 cggtatttta accggaggta ggacattaag agctcgtgtc ttaaaccttg ttacctacta
    19741 tttgggacag ataatgctgg cattcagtat attccttatg ttcatgaagt actgatttct
    19801 ttgctttcat aactatattt atttatcttt tcaagggttg acacacaacg gtgcccttca
    19861 ttgacttaac ctgctttcta tgttactatt ttgtggatat ccgaatatct gttttgatag
    19921 tgggggaacc gacttattgg ggtgaatctt tgtaagatga gtgtcttggt ggctcaaacc
    19981 cgacagctaa tcccatagga ttgcaccaag caatgtagta catgatgatt tttatgcgct
    20041 ttgttaagtt gttgccaaaa acatgatgga ctgcatatga aatatatact gtgggaggtc
    20101 ttatatgaat gttttgctga agattgctat tgttcttgtc gtaggaataa taggaggaag
    20161 ggtagccaga ctggtcaagt tgcctaatgt ttccggctat ctggtattcg ggttgctttt
    20221 aggtccttca ttctttaact ttattagccc cgcggatgtt caatccttca acattataaa
    20281 cgaactggca ttggcagcta ttgctttcag tataggaaat gaatttgtct tgaaagaaat
    20341 gaaaaagata ggtaaatcca ttttcataat cactcttatg gaagtaatag gtgcggtgtt
    20401 attggttttt tgcattttat tctttgtttt cagacaacct ttcgcattca gtatagtaat
    20461 cgcttccatg tcggctgcta ccgctcctgc agcaacatta ttggtaatga agcagtataa
    20521 cgctaagggt ccgctcacaa gaaccattct ccccgtagta gctcttgatg atgtcttcgg
    20581 aatagtggta ttcggtattg ccttgtcggt tgctaagatt gttacatata agcagcagtt
    20641 atcggttgtt tccatgatgt tcggtcctct tattgaaata gcgggatctg taggaatcgg
    20701 cttcgtgttg ggattattat tatcctggct ttcacaaaaa gcctcgggaa gagacgaact
    20761 tcaggcaata gcattggcag ccataggtgt agctaccgga atctctcttc ttttgaatat
    20821 gtctccgctg cttacctgta tagttatggg aacggtttta gttaatatac agcaaaactc
    20881 cataagagta tttgattcgg taaacagctt tgcggcgccg atatatatac tcttctttac
    20941 acttgccggt gcaagcttgg atctttcgat acttaaaacg gtcggtctga tgggcattgc
    21001 ctatgttctg gcaagaggtt taggaaaaat agcgggtgcc tgggcaggtg cagccatggt
    21061 gaaagccgaa cctgctgtaa gaaaatatct gggtttggga ctgcttccgc aaggcggcat
    21121 atccatagga cttatggtac tggtgcgtca gcaacttccc gactatgcgg tagccatcaa
    21181 taccattatc atgttctccg ttctcatata tgaaacaagc ggaccgatat tcgcaaagat
    21241 agcgcttaag aaggcgggag agataggaat tgaagaaaag gtcagaaagc ctcgcagaaa
    21301 aggcaaaaag aaaagtaaag tatcttcaca aaaaagtgaa tcatgtataa tagataaggg
    21361 gtgagattat gtacgttttg ttcatagtat taaatgaaac ctgttgcttg gaagatatcc
    21421 ttgcaggatt tttaagagaa ggtataggcg gcgctacaat tatagacagc cagggaatgg
    21481 gcagagcaat tgctcatacc aaaaatgagg atatacctat gttcggcaca ttaagaatga
    21541 tgctgggtaa tactcatcct tacagcaaaa caattttcac cgtcctgaat gatgaacaga
    21601 tggtggagaa ggccgtatct accgttcgtg aaacagtgaa aggttgtacc tgcagcggtg
    21661 taggcctgat gtttaccgta cctatcggta aagtttacag aatggttgag gaagataaat
    21721 aatctatcaa ttaactgtta aatatagaac ggctgtatta acaagtttag ctgttaatat
    21781 cacaaatcta ttagcaaaac ttgctgttca atctttaata taaccggctt aatgccaggc
    21841 attaaagtta aactttcgtg tattaaatag cataaagaac actttacaaa ctaagtaatc
    21901 tcggatatat tcattgttaa gaggtttatt atgagagcat ccggaatatt acttcccgtc
    21961 ttcagtcttc cttcacctta cggtataggg accatgggta aagcggcata tgagtttgta
    22021 gattttctgg tttcggcaaa acaaaaatac tggcagatac ttcctttggg acctaccggt
    22081 tacggtgatt ctccctatca gtctttctgc agtttcgcat ccaacagtta ctttatagat
    22141 ttggatattt tggagagcaa aggtctgctg ctcaaagaag agtatcagaa cgagccatgg
    22201 ggcgaaaatc ccgacaaggt cgattacggc ttgttatata ggaagagaaa taaggtgttg
    22261 gagaaggctt ttaacaggtt tgataaagaa aatctctcat ttaaagcctt ttgccgggat
    22321 aacgcatatt ggattaatga ttatgcaata ttcatgtcgc tgaaagaaca ttttggcgga
    22381 aaagaatggc agtcctggcc ggaagatata aggatgaggc ataacgatgc tttggtgaaa
    22441 tacggaactc acctttcgga aaaaatattg tttcataagt tcttacagtt tgagtttttt
    22501 acccagtgga cacaattaaa aaaatatgca aatgaaaaag gcataatgat aataggcgat
    22561 atacccatat atgtatcacc ggacagttcc gacgtgtggt ccagacatta tctgttcctg
    22621 accgatgagg acagaaatct tacatcggtt gcgggagttc ctcccgatgc tttttccgcg
    22681 acgggtcagc tatggggaaa tcctctctat aactgggatg ccatggagca ggaggatttt
    22741 tactggtgga aacaaagaac aggtatggct tctctacttt atgattatgt acgcatagat
    22801 cattttatag ggattgagcg atatttctcc atccccgccg gctccgatac cgctgcaacg
    22861 ggagtatatc atcagggccc cggaatgaaa ttgattgatg caataaacca atccagaggt
    22921 aacatgggta taattgccga ggatttggga gtgttgacgg ataatgtgag aaaattgttg
    22981 gaaaaaaccg gttatcccgg aatgaaagtg ctgcagttcg gtttttccgg agagccttcc
    23041 aatgacaatc ttcccatgca ctataagaaa aatcttattg catataccgg cacacatgat
    23101 aacgatacaa tactcggatg gtataaaaag ctggacagaa aaaacaaaga cttcttaaga
    23161 aaatacttaa acaggtcgcg tgttcatgtg tacgacttga tttttgaaac aatgaaatcc
    23221 gtggcaaata ccgctgttgt acccatacag gactggctgt gtcagggttc aaaaagccgt
    23281 attaattttc cttccgtagc tttcggaaac tggacttaca gaattaccga taatatgctt
    23341 accgaatcgc ttgcacaaaa gatttccgac atgaccacac tctgtgccag ataaacagtt
    23401 atttctcgca ctttgccaaa tagtccgata tatcctcggt gaatactttc accaggtcgt
    23461 cggcttcttt ccttgagtgt cccattgcat ttatatatac tttcagtttc ggttcggtac
    23521 ccgaaggcct taaagcatac caggaaccgt cttcaaactc atatttaaga gcatcctgag
    23581 cgggcaaccc ctcatatccg ttgatgtagt ccttacatac cgaacattta atgccgttaa
    23641 tttccagaag aggttccttt ctccattgtg acattacatg ctttttgact tttgctcctt
    23701 taataccggg atattccaca gatatctggt tttccgtcat atagccgaat ctctcatata
    23761 tatcggtcag cacttcataa agagttatac cgcgcttttt gtaatatccc gccatttcac
    23821 acatcatcat ggccgcactg actccgtttt tatctcgcgt aaatgtttct atacagtatc
    23881 cgatgctttc ctcataaccg aatacgtatc tgtattcacc ggtcttttca aattcatttg
    23941 ccagaccgca tatatatttg aatccggtga gcgtttcaaa gaaatgtgtt ccgtaatgct
    24001 ttgctatttt gggtacaacc gaaccgctta ctatggactg cacaacagca tctttcggcg
    24061 aatacagtcc tttttccttt ctttgcgaca acatgtattc cgcaagcaat gcacccgtct
    24121 gattgcctgt gcagaagaca aactcattgt tgtcattctt aatcatcatg gctatcctgt
    24181 cacaatcggg atcggatgct attatcatat cggcatccgt ctttttgcca tatttcagag
    24241 gtatttcaaa aaccttaaca ttctccggat tgggataagg agtggtgggg aatgttccgt
    24301 cgggttcttt ttgctcttcc actacggtca cattgtggaa tcctctttct ctgagtatcc
    24361 ttgttaccgg aaccaatccc gtaccgtaca gaggtgtata aacaatttta acatccttgt
    24421 ctatatcgtc attcaaagtt aaggacagaa cctcttcgat gaacatatca tccatgtcct
    24481 taccgagcat tgttatctgt cccgatttaa ccgccttgtc aaagtccacg gtttttacat
    24541 cgttaaaagg atctattcgg gaaatgtttt cggctatagc atcagctatg tcgtccaata
    24601 tctgtgatcc ttcttcccag tacactttat atccgttata ctgcttggga ttatggctgg
    24661 cggttatgtt aataccggtt gcggcattca gtttccttat cgaataggac agctgcggtg
    24721 tagatctcag actctcgtac agatacacct ttattccgtt tgccgccaat accgttgccg
    24781 actgttttgc atattcatcg gatttaatcc tgcaatcata cgctataacc gctcctcttt
    24841 tctccatgcc ctgctctata acatatcggg caagagcctg tgtggtctgt cctattacat
    24901 acttgttcat gcggttggtt ccggcaccca ttacgcctct caatcccgca gttccgaaat
    24961 ccagagtttt atagaatctg tcttctattt ctttttcatt tcctttaata gcttccaatt
    25021 cctttatagt atcggaatcg gcttttttta accaatcatt atacttatcc ttgtatgtca
    25081 tctttgtacc cttcctgaac cgtctccttt cataagtctt tccacttccg ccaccgtgaa
    25141 tcgtgaaaaa tcgccgtaca cggtatgctt taagcaactt gcggcaaccg caaactccag
    25201 tgctttttta ctgtcaccat atgtgttaag tccgtatatc agtcctgcgg caaaagaatc
    25261 tcctccgcct actctgtcca caatgtccgt aatctcatat tttttgctta cataaaaatc
    25321 ttttctgtcg tttatacatg ctccccatcc gtttatattt gcatttctgc tttctctcaa
    25381 tgtgatggca atgaccttta tattgggata ggtcgacaga actttatcgg aaagctcctt
    25441 atatttggaa tcatccaact cgccttttgt aacatcggca tcgttgtgta tctccagggt
    25501 tttttgtaca tcctcttcat ttgcaacaca gacatctgta aacttaacta tgccgggcat
    25561 tacctccgat gcctttttac cgtatttcca cagatttcct ctgtaattaa ggtctatgga
    25621 taccgtaagt cccgcctgct ttgcggcagt caccgcgtct aaagtaacca atgcgctgtt
    25681 ttgcgtcaat gccggggtaa tacctgtaac atgcaaccat gaagccccgt taaatacctt
    25741 tttccaatca atatctcccg gttttaccag ggatatggcg gaatactctc tgtcatatat
    25801 tacttttgag ggtcgctgca ccgctccggg ttccagaaag tatatgccca tacgtccgtt
    25861 tctgtaataa atgtcgtctg tatttactcc gaatcctctt aactgcctga cgcatgcttt
    25921 tgctatgtcg ttatccggca gaaccgttac atatgatgta tccattccga aattggcaca
    25981 ggaaactgct acattggctt ctcctccgcc gaaagtggct tccagtttat ccgactgtaa
    26041 aagcctctct ccgtaaggag atttcagtct cagcattatt tctccgaaag taactatctt
    26101 acccattcaa ctcctccttg gcctgcttta tactttccaa tatctgtccg gctatggcgg
    26161 tcatcttttt atactcaccc gtcttggcat actttgttat atttccgccc actcccactc
    26221 cgcatgctcc cgcttttatc cagtcttttg cattattttc gtccactcct ccgttaacca
    26281 taagattgag atacggcatg ggacctttta ccgcttttat gaacttggtt cccaatactt
    26341 ctccggggaa tatcttaact atatccactc ccgcttcggt agccgttact atctctctcg
    26401 gtgtcatgca tcccggcatt gcaggaactc tgtaacggtg acacatggtt attgtaggca
    26461 aatccagatg tggtgcgatt atatacttag ctccggacag tatggctatt cttgcagtct
    26521 cctcgtccat aaccgttccc gctcccagta tcaagtcttt ttcatcgtac tttttcgaca
    26581 actcctctat tatgcggtga gctccgtcga cagtgaaagt tatctctatc agcttgactc
    26641 cgccttctaa acatgcgtct gttatcctgt atgcttcttc ggcactttgc gctctcacaa
    26701 caactatgag tccgcttttc attacatttt ctattacctg cagttttttc atttagtttc
    26761 ttcctttcgg ttatacatcc ttttcattat atcctaatct tcaaaaaatt cacattccag
    26821 catggcacat aagcagtgat ataccggaag gtgcagttcc tgaactttgt atgtttttga
    26881 agcgggaact tttatgcaca catcggccag cgaattgagc tgcccgcctg tttccccggt
    26941 taaacctatt accggtattc tgcatgccct tgcggttatt gccgcattat tgacattgct
    27001 tgcattgcct gaggtactta tgcacagtat gactcccgca agttttctga aagcataaac
    27061 ctgctgagca taaatcattt caaatgaggc atcgtttcct atggcggtta tgagcgaggt
    27121 atggctggat aagcagattg ccggcagtcc ttccgaaagc ctttcgctta ttgattggtc
    27181 cagcatgggg ttgggttcaa tcggtcttct tttattgaat cctttcataa gttcccccac
    27241 tatatgctca ctgtcggaag ccgatccgcc gttaccgcag ataagcagca ttttgccgta
    27301 tctgaatgtc tcctccgtta tctcaaatgc cctcattata tcagccttgc attttttcaa
    27361 tgaagtataa cttctcaaca actcttcaaa aacttccaat gtctctcttt tcatatcaat
    27421 tacctcttat cagttgtttg ccacataatc cgctgcaacc gctaaagctt cataatctcc
    27481 tatggcatct cccagtgaag aaggcagaat atcacatacc tgtgaggata ttgccagact
    27541 ctctttactt aagactctct ttgcatgcgg ccaaagcaga tttctttctt ttgtaaatat
    27601 gcttcctatt gttatggcgt cgggattcaa tacatccaca agtattgcca atgctcttcc
    27661 caacatctct ccgctccttg catatatcgc ttttgcaaga ttatcaccct ttgctgcggc
    27721 ccttgccaca atcagcgcat ccagttgctc tatttcggtc tcactgctgc agaaggatac
    27781 ttttccaccc gattgcagct cttcccgtac catagatttt gccagttgtg cgattcctcc
    27841 gcccgaacag aagccttcca ccgaacccat tttcccatat cctacgggac cgtaatccgc
    27901 aagtcttaca tgtcctatct ctcccgccat gttgtttgtt ccggtataca gtttaccgtt
    27961 aagtatcatt cccgctccga aacccgttcc gaatgtgcag aatatgaaat ttctgtatcc
    28021 gaatcccgaa ccccataacc attccgccag cgctcctgca ttggcatcgt tctgtaaaac
    28081 ggcttttact ttaaatcttt cttcgaaaat atccgttatg ggcacactgt cccagcctat
    28141 aaggttgggg ggtgaaagta taaggccttt ttcactgtcc aaaggacctc cgcaacttat
    28201 gcctatgcag gataaatcat tactaatcaa aaagtttttt gcaagtatac tctccagacc
    28261 taagataagt ctgtctatta ccgcggacgg tgagtcacag gatttggtct tgaatgaaat
    28321 cttgtccaca atgtccactc gaataatatt attgtctacg cattttccca ataccgccgc
    28381 acattttgtg ccgcctatat ctatcccagc aatatacatg acgtacctcc gtcctataaa
    28441 tgatccgctt caattatatc atctgtacag ctacattagg aaattctata aagcttaatt
    28501 ttccgccact cttcttaacc ttctgtacag ctcttttgcc tcttcaacgg tcttgtcggg
    28561 tcctcttaac cggaatatga tatttctttt cttgaacggc catgtcttgg aagaaagtat
    28621 cttctttgct acatcggtgg ataatcccaa aggttgcaga atggtcaagt cttccggcac
    28681 attttccata gtctcctcaa tatcatgcaa gaggctctca taatcaatac cggtaatgcc
    28741 gggtattttt gccatttctt ctattacatt ctttgccgaa ccgcaataat gtattctggc
    28801 cccgttcagt tctctaataa accttacatc atgtttaatc acatggttta aatacagctc
    28861 tcttcctatc atatgtaaag aacagttgga tattctggca ttacctttcc ataaagctga
    28921 ccagtcatac agccatccct ctttcgcatt tgtccactgt cttgtggctt ttgcatattc
    28981 tatggtggca tcggtaacca cctccataag tttatcaaag gcttctatat cgtcatacat
    29041 atcaaaaaat atatcgttgc ctcttatgag gtgtgcattg ttgaacggtc cctgaatatc
    29101 gggaaattcc atatggtatc cttccggcag tttctcaaca aagtattcgc tccattccat
    29161 cacttttctg taaggagcaa cattgatatc gggagctttc agagaaaaaa tctgttcccg
    29221 cgttttcacc acatggtctt ttgcacaggg cagactgtta gtcggataga acacttcaca
    29281 gccgaaagcg tttgcaacaa cgcatgttcc gaaatccact cttacattca aagtggaatc
    29341 atctccgact tcaacatggc gtgcatttct ctccagatga tttttaagca ttacatcttt
    29401 atcggcaagt atttcctccc atgtgggatt aaacgaagtt tttataggaa tagtgaattt
    29461 aggtatgcag tttttaggat cggtaaacat ttgcatatat tctttttcca gctcttcata
    29521 tcttcttaat tgtgcatcat taagagcata aaccgatgtg tccagcataa gccgctccct
    29581 tcccgtactg aataaattat accattacct gctgttttat acattatgtc attgacgttt
    29641 ggaatatata ttccggaaaa ctgcgtataa tccgacatga catgttgcat tatacgaaat
    29701 aatgcattaa aatatatgtg gaatataaat atacaaggag gttaccatga actggattga
    29761 cagaaccatt acaaatcatc ttcttcatgc aatggatttt ttccctgccg ttttattaac
    29821 aggtcccaga caggtaggaa agtcctcttt gttacaaatg gttgcaaagg attttgagta
    29881 tataaccttt gataacgccc ttattctgca acaggcaaaa agcgatcctg agttgtttct
    29941 tatgagccat tcaccaaaat tgattcttga tgaaatacaa tatgttccgg aattgttttc
    30001 ttctttgaag ataagaatcg acaatatgag atttgaggca ttaagtaaca acaaacccag
    30061 agaatgctta tatctgttgt ccggttcgca agcatttgaa ttaatgcaga atgtcagcga
    30121 atctttagcg ggaagattgg caatattgcc tcttcaaggg atatcttatc gtgagcaaaa
    30181 aaagatatcc tgtgcacttc cttttcttcc taccgagcaa tatctttccg tccgcgaaaa
    30241 agaaagcgta gatttatcag acctttggga aataatacac agaggatata tgccgagact
    30301 ggtttcttca aacgatgatt gggaaacctt ttatgcaagc tatgtttcaa catacattga
    30361 gcgtgatgtt aacaagattt cacatattga tgataaaacg gattttgttc gcttcatggc
    30421 atcggtagca gcaagaagcg gcgaattatt gaactatgac agtatatcga aagatatcgg
    30481 tgtttcttcc acaacagtaa aaagatggct tcaaatactg caaacagcag gcatcatttt
    30541 cctgctttat ccgtattaca gtaatcatct gaaacgtatg ataaaaacgc ctaaagtata
    30601 ttttatggat acgggattaa tggcttggct gacaagatgg gttactcccg ataccataaa
    30661 atacggagct aaagcaggcc aattctttga aaccttcgta gtttccgaaa ttataaagtc
    30721 attacttaat gcaggcaaat ccacacgtga tttatatttc tacagagatg ccgaccaaaa
    30781 agagattgat cttattatcc aggacggcag aacaatatat cctgtcgaga ttaaaatgac
    30841 tgccagacct cataaaaaaa tggcatcggc tttcagtgtt ttggaatcag tgagggcgga
    30901 tgatatacag attggaaacg gagcggttat taatctgtat cacaaacccc tgttgattga
    30961 cagaaatgta ctttccatac cggcaggata tttgtaaatc agctcacgct aaactccgtt
    31021 tcggcggtta catcctttcc gattactctt aaaaccgcct tgccgctttg tttatcggtt
    31081 tttacatata ccccggcaca acctcccttt agcgagataa gttcgggacc tataagcttt
    31141 ataggacctt cggtaacaat tcgtaaaggc tcgttgcagt aatttaaaag gttcatattc
    31201 tgatccaccg cccttatggt taccgatgcg gtatcgtaag tttcttcttc tttcaattca
    31261 tgagcatctg ccgataagac taattgcatc ttattcatag gttctttaat ttcggttatt
    31321 accgtttttc cgtccttaat accttcaaac acatactgtt ttacattgtc tccccaaccc
    31381 gccacatgag cagtgattat ttccgtcagt ttgtttattg taagtttttc ttttataagt
    31441 accgtcacaa gcatcagcat atatccaagc ggaagatttt ccggtccgtt tacatatata
    31501 tatgacaaaa gttttttaat tacccttgat gaatattcgg aaaaatgcaa atcgtcaatt
    31561 aacatatttc ccgtgaagtc atctatttct ataggagggt gtaataaatg tttgaatcgc
    31621 tttctgtcgg gataaaagtc ttttatatgc tttccgttta tgctcatctt tatataatcg
    31681 caattggtga aggcccatac gctttttgtc attgccatgg gataatcgcc tttcgacatg
    31741 gatgtggcta catgcattac cggatattta tcggtctgtg atgcgtatac atatgcggca
    31801 tctttcggat ttctgaacat gtccagcacc ccatgataac atatcctgtc accgcttccg
    31861 aaatccttat gtgtattgta gtcgttcatg caccagccga aacttcctgc cacaccgtca
    31921 tctcccatga cggcattcag cactcttgcg tggcgtaggg catgttctgt tctgtgcggt
    31981 tggtcatcaa acattttggt aggatacata tgtccgttat actcggttat gagatacggc
    32041 acatcacctt ttgtcacatc ttttttcttt gctattacct gctttgtacc gttatgggta
    32101 aaatcattgt aggtatagac atcctccaaa aagctgcttt ttttaataca tctcactccg
    32161 ccggtctgcc tgtacgaatc atattgctta gcaaccttat tggtttgcct gtaaaagtcg
    32221 tcatcatcag gtgattcgtt tattctgacc ccccacaata ttatgctcgg atgatttctg
    32281 tactgtttta ccatgttttc ggtattttcc acggcctttt tcttccactg ctcatcacct
    32341 atatgctgcc atcccggtat ctcggtaaac accaacaacc ctatttcatc acatctgttt
    32401 atgaaatgtt tactctgggg ataatgcgat gtcctgaccg catttacatt cagcatattt
    32461 ttcagtatat cggcatcatt tatctgtact cttttgctca ttgcatatcc tgtatatgca
    32521 taagactgat gtctgttaag ccctgttact ttgacctttt taccgttaag ataaaaaccg
    32581 tcattcttaa aaatcgcatc acggaatccg aaccggtcac aatactcatc tgaaagcttt
    32641 ttgttacggt aaagttttac tgtaattcta taaaggttag gattgtcgat atcccagttt
    32701 tccgccggga ctttttcaga caaatgaata atctctttcc tcaccggata gtatttttca
    32761 aaaacacatt cattatcttt aaccgcaaag attgaaagtt gtatctcaca atcctccgta
    32821 cagttaacta tttgcacttc cgcaaaaaac tcgcataatc cgtcagccgc tttggcctta
    32881 ccggatacaa acacatcgga aatgtgtgtc ttctctttaa tatcaatgta tgcttccctg
    32941 taaatacctc cgtaagtcat ataatctata acattaccga agggaggctg gtttaatgtt
    33001 tcataagcat cggcaataac agttattctg ttttctttcc cccattcaat atattgagat
    33061 atgtcaacgg tgaatgaagt atatccgcaa ctgttactat atacatgcct gtcatttata
    33121 tacacctcgg cttcatgagc tactgctgaa aaagtgagta atatagtttt gttttcccac
    33181 gagttatccg catgaataat cttttcatat ttcgatattt tctgataaat tgcttcgtca
    33241 aaacaattat atggcatatc gacattactg tgcggcagtc gcacggtaaa agattcggaa
    33301 gattcaaccg acggataaaa ttgccagtca tcatttatat gtattctcat agtagcctcc
    33361 ttctgtcaga ttgctttttc tttttctgta aagacaacaa ccaacaggga gcctgcggcg
    33421 gatattatgg ttgctatcaa cagcattgaa ggtattgata atgcatctat caatgttcct
    33481 cccagcagac ttccgaaaac accgcctaat gtcatggttg tagtcagata tgcctgacct
    33541 tttattctgt cattatcccg catcaggttg tttatataat agactgttgc cggaacaaat
    33601 acggcatagg aaaacatctg taatgaatag ctcgcatata tgtgattaat attctttgat
    33661 aaaagaattc ccagtttttc tattgtaaag aatactgcgg agatttgtac cagcatactg
    33721 cattttaatc tctttgatat tatgagatac aaaatcatac ctataacttc aaatattgca
    33781 gctataccca acacgtttcc cagttcggca gaaccgtgcc ccaatctctt tacgatatta
    33841 ataaggaaat ttatattcat gttgtggctt gtaaatatca gggttgaacc tattaaaagc
    33901 ccggtaaact ttttatactt cgcaaaaaac ttaaacactc ccgtagcagg tgcatgcgca
    33961 tatttgataa cttcggagga tatgcccaca tcatgtgcag gcttgaaaga cagtgtaacg
    34021 ggaattaaca gcaggaacat agcaaagtat attacgggaa ccgaatccgc tcccaatttc
    34081 gcagtaatat atcctacacc gaaagaggca aaagccgcac ctaacgaacc gacagctctt
    34141 gcgatagtga aattgagttg tatgcctttg ttggcgtatt ccatggcaaa ggaattaatg
    34201 aatgagtgca atgtatgtac gaaggttatt atgagcaata acaatatggc tgttagataa
    34261 ggataactcc ttatagccac aatcaaaaca gtcaatacaa caattgcaga ggttaacgcc
    34321 aatattattc tctttaattt cgactcttcc gctttgtctg cataggccgc tataggcggt
    34381 tgtaataagg ctgccaatat gcttgctacc gcaagtatga ttcctgtctg tgaactgtta
    34441 aatcctttat ccgaaagaaa gtatgcggca taattgtata cggttataaa gcccatccag
    34501 tagagagcct gcattacccc gtactttata ttcaacctca atgttacctt cttcagtgac
    34561 atgttcacct ctatgaaaat tgtaacagaa aaagataaat agtctataag gagttgatta
    34621 acagaatatc ttttatgttt ttagaatgat tacttgtctt taagttgtac tcacatacaa
    34681 aacataacat attttccgca ttgtaaagag gtacggatac tcaacagttt cattgaacat
    34741 tgtaaaattc tatcactcta ttcatattga cattatatcg gcgcaccgat ataatgtgtg
    34801 tgataggagg taatcatgat gcttcaggaa ctgcttgaaa gagaaaatat gactctgtat
    34861 aaactgtcaa aaaacagcgg tgttccttat actaccgtta atgacatata tcacggaaga
    34921 accggcttgg gcaaatgtac ggcggaaacg gtgtataaac tgtccaaggc acttagtgtg
    34981 agcatggaag aacttctcct gccttatctg atagagcgaa cagactttga gctttataaa
    35041 agcagtgttt gtcatagatt aaaagaatcg ggcgatatgg cttttattgc cgaaactctt
    35101 cagcaagaca atatcagtaa gttataccga cgcaaaatgt atccggagag tctctacttg
    35161 cttgctatgc ttgactatgt gagtagagaa aacaatattc ctttgtgtac aaagtacgat
    35221 gcattgcgca atgttaaact ggaggaaacg ctttatcccg ccggagtact tgtcatgaat
    35281 acggtaatcg gaaacaataa aattaaggaa gatgcattat ctcaagccat tccggaattc
    35341 atgcgccaca atattgtaga aagcgaggtg cgtaatgtca tctgatacag tttattcatt
    35401 taataaagac aatttagata tttatttaaa agagttggca aagctctttc gcaaacaaaa
    35461 cggcaagaaa gctacagcgg aaatcattct ggtaggcgga gcagccgttt tggttaacta
    35521 cggttttcgt aatatgacag cagacataga tgccatcatt catgcttcct cttccatgaa
    35581 agacgctatc aaccaaatcg gtgataagta taatttgcct aacggttggt tgaataccga
    35641 ctttatacgt accgcttctt attcagataa actcaatgtt ttttctgtgt attataaaac
    35701 cttttacggt gttcttcagg tgcgtagtat atcagcagaa tatctcattg caatgaaact
    35761 taagtcaggt cggaaatata aaaatgatat gtcagacatc ataggcattt tggcagaaca
    35821 taaagaacgt aaagagcctc tgacttggga aaaagtaaat caagccgtag tagatctata
    35881 tcaagactgg gacggcatta ctgaggaagt caaagtcttt atcaagaatg ctattgacac
    35941 cgataactat gatacagtgt ttcagcaaat aaaagaacag gaacagctct ccaaaaatat
    36001 attaattcaa tttcaacagg agcacccgca tactgtaacg gaatcaaatg ttgacagtat
    36061 attaagaatg cttgaatcca aaaagacatc cgatgctctt gagtaagaat aaaagttttg
    36121 tctgaaatat cacaattaat gtgaaatcaa aacatttata aggtgcatga aaagaaatgt
    36181 tttgagacca atggtgctcc aacatatcat cctcctataa aaatcagcaa tatatacttt
    36241 ccaaatgctt agataaatta aatacactat gcatatacta aactatacta cctattgcat
    36301 tcaatgcact ggcaagttcc ttctttggtc tttttatttt tccggtatag ccgagattgt
    36361 gaaccaaaat gtcaatacaa tcgttgtctg aaagttgagt tataacatca agctttagcc
    36421 tagcccttga agttccaaca tagtaaatca gaacattctt ggaattaaat gaatcgctat
    36481 caacatcaat aagaattatc gcatctgctt caagtccttt aaattttcgg catgttgtaa
    36541 aacgatattt gtttctatat aaaccatctt tacattttga attaaatacg ctctcctcct
    36601 cagtcttgca agttaaaata actatgtcgg aaaaaccatc cttgttcaag ttgttgattg
    36661 tatggtcaat tcgtgccaat acactttcag gggaagagca aaaatggatt tttgcaggta
    36721 ctccttttaa gcatccctca atcagaagtg gctttttgac agttatggga cgcaaagaag
    36781 tagtagcaat attttcggta tttctgcaat ttttgtagag agtcaattta cagtctgctt
    36841 cttcaataaa tttcggaaaa tgatttgctt gaatcatctg taacttatca taaaagacat
    36901 aaaaagtacc aagtggtttt gtatcagtta taatatcttt catcaactgc agaatatcag
    36961 cctcatttat tgtatcaatt ccgaaatctt gcccctcatc aactattatg tgttgataag
    37021 gaaaagactc cgacaaatac atatcttcaa gagccgcttt aaatctttta tagtcaggta
    37081 cagcagtatc acatacttta caagcgagac ctgcaacggt gtagaaatca attttttcat
    37141 tttgatagca cttactaaga tgggttttta actggttatt aaagcataaa aataaaactt
    37201 tttcacctga tgaagcatgc cgttgcgctt tttcaatagc aatcattgtt tttccagttc
    37261 ctgccacacc atttataact gctgttctct gttccgtaag gaaatttaat attccctctt
    37321 gttccttcag aaacctgtga aaaacaattt tcttcaagtc gttttcaaat gaggctgacg
    37381 ggaaaacatt gaactgtgga cagaaaattt ctcgtataat tttcttctct tcatattcac
    37441 ttaaatccgt ggttattcca ttgggtaatt ctgtgctgaa aattttattc aaatactcat
    37501 ccggactttc aagtgcttct tttgtcataa tcagatttct atcagcttcc ggtggaagtg
    37561 tcatagtcct caaccttata tctgttaccg atggaaacca tacaccatgg agaaatttgc
    37621 agcgactcaa tatcccggaa caactgcttc tttcaatata gcgaattaat ttatatttat
    37681 tagacgaagc ttgattgaac ggaccgccat tatgcatagg ttctccactt gaataatacc
    37741 agtaaccgtt ttcatatata acttgtccgg cttttgcctc gatacaaagt acgccttttc
    37801 tgcgattaaa tatcacaaaa tcagtttcac tctcatgaag aatattctca gtcacactgg
    37861 caattctaaa tgagtggaaa acataatagt cgtcaggtaa taatttcaat gcatcaaaca
    37921 ttaatccttc aaggcttact gaatcaaatt ggtgaggctt ttcaggaatc attattgcca
    37981 tattatttcc cccctattgc gtttaaaata tcagttgcat caattatctt attgtcaccg
    38041 caaaaacatt tccacttctc ggtagtaatt ttttcgaaag tatctttgta atccgaagaa
    38101 aaatacataa ctttagattt ttcccacaat aatgttgccc tatatttttt tgaatcccct
    38161 gcaataataa atgagcactc ataaaccggt ttttcctttg aatcacatat atctgcaata
    38221 ttaatcaatt tatcaagcaa agaccgttct gtcagattat ctgtccattc tttaactgat
    38281 ttccatatat cagcataact gacatcactc atatcggtac ccttttccgg aataatcact
    38341 tgaagatttg tttttctgtt cgctgaagaa tcctctgtat gtgtaacaaa actttcccat
    38401 cgcctgttac catgcaattg ttcataaaaa aatccgagac ggtagttatt aatcactcct
    38461 gcaacagttc ctctgtcaag aaagatgttg aattcttcgc aacttgtttc aggaataagt
    38521 gcacatgaat agcatgctgc taaattaagc gaatctcttc cttgtcccaa acttaaactg
    38581 caaaccggat catttgaaca cacttcgctt gatttaattg cctttttaaa aatgttcgga
    38641 aatgtatccg gatacccttg gcgaacaagt cctcccaatg ttccttctga atctccgctt
    38701 gcggtataga taagtatacc cgccatctct tttccttcac ttccctcact gcaataaatt
    38761 ctttctttca atgaggcaat actatagccg cattcgaaac tcaactgttt aatcaaaaga
    38821 tgtgaaatag tatgcaaaag aacatactta cttgtcactg tacgcgggtt accagagctg
    38881 ataaatgatt tgctgtaatt ctcgttcagt ctgtctattc gtaattgtaa atcacggttt
    38941 tcgctacgcc aattattgat tgcttcttga ttgaattcaa taaaaatgcc ttcaccgcgt
    39001 acttcatatg caggatacca gtttgttgtc ggttctttta tcggtacaac attcacctgt
    39061 ttcccaacag gttctgttct gtcaacaggt ctgattcttg aaaaccctgt caaagcttgt
    39121 acttcacgaa ccttatgaat cagtgaaata cgcttaatat atggcaaatt gtatgcaacg
    39181 atatcagttg attctctttg aaaatcgcca tatatgtctt tacctaacga tatttctccg
    39241 ctcaaagcgt catattcttc cgcacgatac tttacactca atgtactgta ttcttcttct
    39301 gaaggttggc gccattttct ttcaagtact tgttttattt tttcaggcga tacattgatt
    39361 tccaggctca tctggtttga gtaatggtca atttggttaa caataatggc atctttactc
    39421 tcctgtgcgg tgtagggatt acgcataaat gcagaaataa cgttcttgca ctcagaaaat
    39481 gcagagctct cctctatttt tctagttaac atacttgaat acggtggaat cactaatgaa
    39541 cttacgatga cgggaaagta tactgaggaa ctgcctcgtt gcaagacctt cgggtagagc
    39601 gagcaacttt ccttttgatt tttccaagga tgccttcccg agcaagtgaa attgtagtaa
    39661 ttgttgtgtt ttttatcaag ttcttcaaat atttccctgt caaaagcttc ttttaatgtc
    39721 gttttcgcgc cgcaagtttc acatgttata actaacccct ccagaccttc cgttgaagaa
    39781 gctccagttt tgaaagtaag tgacgggcga ttacaaataa ttttagcccc gcctgaattt
    39841 ttacaatgaa cccatttaac ccatggaaaa tcatcaatat gtccatgctc acatacagtc
    39901 actatacgag ctactaccaa gtcttgattg catatcggac aacacatatg cttaaccata
    39961 tacggatctg attctattgc tcgttttcga ccctttcttt tgtactcatc aatccagtgt
    40021 tttataggct gaaactttct gcatttcgga caaaaatacc actcgggaaa gcgagcatac
    40081 gatatgcctt ctttacataa ttcattattc ttacctgccg gtatcccaaa aaaatctaca
    40141 cggagggctt tttcaagtct ctcatcatgt atttctataa tttgttcacg ccagtattcg
    40201 ggagcagcgg tcattaaggt ctgatcctta aaatcaacca tagcaccgac cccatattgc
    40261 aatacagcct gtgaggctct gacagaatgt gtaatatgat ttaaccgtat tctttcagca
    40321 ttttcgagtt ccattttatt tttcctccca aatcagaata ttacctttga ccaaactgtc
    40381 gacattacgc atagatgtca ttgtatcaaa agctgcatca taatatcccg aattataaac
    40441 tttcatcaaa cgggcttctc cgtctttagg actttttatc atatactttt cgccgtatgt
    40501 gaaacaatct tttccataac tttcagccat tcgttcccat tcgtctacaa ttctatctat
    40561 ctcttcagaa ataagcacac tgtcacttgt catccccgaa ttcatgcgtg tgatgattga
    40621 agcatcacga tttataatgt agtcacgaat tttttcaatt gaatcagtgt atttttcttt
    40681 tgaaaaattt gcagcaccat tttcaggcaa aagatctgta tttgtgtgcc tgagcaacgc
    40741 aataactaca gcatgcaatg ctctttctct tgcaggatta gaaaacggtg tagcacctgt
    40801 aggttcaaca tatttgtaaa aggattcatg atagggtttg aattgttcgt aatgtgatcg
    40861 gtctctactt tttgagccat cgtacaatat aaaagctata ccgggatttg agcgacctat
    40921 acggcttgat gcttgaatat attcacttgt caacttgggt tgaccgacca acaacatcac
    40981 attcaagcgt gctacatcaa tacctaccga aatcatattt gttgccaata gcacatttga
    41041 tgcatatcgt ttgttttcga tattttcctt agaatattcc agtttttcaa gtttatcaag
    41101 agtttcattc aattcagttg ttgaaacgcg acttgttaat tcgtctgcac cagctatcat
    41161 tcgagcatct tttgccgtac cgagacgata cgcagttcgc ttaataaagt cttttacgtc
    41221 atcatcaacc aatgttgagc atttcccgag atctttaaga ctgttgaaat acactgttaa
    41281 agtccagaat ttatccttaa tactgtcagg caaatccatc acattaattt tttgcagtaa
    41341 tgctgcaata gaacgaacct gcatcattgc ctttgtttta ccggatggca tcaaaccgat
    41401 atatagcctt ccatactggt ttatatcata gttaatattt aattccctcg caaaaaaaga
    41461 atcttccgca tcaagcccgg gttgcgggaa ctgagcgacc tcacgattgt atagtgcaga
    41521 gcattgttct gctgctcttc tgattgtcgc cgtagaagca actatttttg cttttatgcc
    41581 cttagaactg cacaaagcat caattgctgt ctcatagagc ccgaccatcg tgccaagggg
    41641 accggaaatc aagtgcaatt catcctgaat aattaattcc ggcgaacgat tactgttgtt
    41701 taatccgaaa aatgagccga tttctttttt ccatggcaac atagcaaatt tgtcaactgt
    41761 accaaacagc aatgtaggtg gtgatttgta caattcctcg tcaattattt gtatcggcaa
    41821 tttgccaact tgattaaaga aacaactttc ttgaggacaa aacaactcaa aatagttacc
    41881 atttctcatc ctataaccga attgtccgtc aaggcgctgg ttaattctgt ttttatcttt
    41941 aactaatttt gttccacacc aaggacattt aagaacttga aacttattat acttgtcctt
    42001 gttttcacga atattgtcgg ctgtggattt aattaacttt tctaaacaat cttttgcttc
    42061 tttgttctta ttaggtgtat gttcgccccc aatccacaat ccgattgtta tatcatcctc
    42121 tccaagcgga tatgacttat aacgagggtt tcttctcaaa gagtctcttc gtatgtactc
    42181 gcacgcacaa atcaaggtcg atgctctcgt aaactgttgt gcagccaaca gtcgtaaagt
    42241 atatctcata ataacagaag tacctgcata actgtcggga tgtgcaagcc ttctgtaaaa
    42301 tatggtaaaa gctgttaaac caagatatgc ttctgtctta ccaccaccgg taggaaacca
    42361 aattaagtca acaatatccc gttctgcaga tgcatcttcc gataccgaat ttatactcat
    42421 taaaagaaat gccaactgga aggggcgcca aaaatattta tctgcagtaa tatctttatt
    42481 atctccgtaa tcaagcttgt ttaacatatc cgatatctct ggatcatcag gatagcgatc
    42541 aatatccgag gttacttctt gtagtttcag atgaattctt tgcatcaaca ttgctctatt
    42601 ggccaatgca aatgcattcc atttcatttc ggaggactcc agtgctttta ttcctttttt
    42661 aattcgatta tatgagattc ggcacttggt tatattggtt tctgcaatct tattatatct
    42721 tccgttatat tcagcatcta taactgaaag cttttcagtc agttcatcaa tccatttttt
    42781 gtatgcgtca gcaaatatat tcagaagttc tattttctct tcctttggag agtcacaaaa
    42841 gtctgataag taacgcattg acagtgtttt tttctctact gcatattttg gcggtaattc
    42901 aaaatccatt tgcggaactt ctaacatagg gaaaaaatcg ctaaataatt ctccattacc
    42961 atttccgtca acattccagt taattgatgt gccaagccct gttccgtaaa cttttttgtt
    43021 acgatactga agttccaaag attgctcttc ggaatcaaga gcagaaaact cagccacacc
    43081 ggaataatcc ctaaaagtaa aactattatc atatgaactt actcttattt caggctgaaa
    43141 gacacaacgc aatccgttag atttaccctc caagtcatta acaagcatta tagtaatcga
    43201 gtataagtca tcactgattc tttttcgaag ggcagttaat tttgcttttg tcccatccaa
    43261 ttctttgttt ttatcaatgt aatccgaatt cccaaatttc agtttaactt ccagcgaatg
    43321 cggtacacgt acaaaccctc tttgtccttg atcggcaagt ttgtacatta aatcgtaaat
    43381 gttagattcg tcaaacttca gaaggttctg ctttttcaga ttatgaattt ctcttctgct
    43441 gattttactg tctttgaatt taaggcacct ttcggataca tcataaacta aatagttact
    43501 tagtggttca ggtacggtat atgtctcagg attttgagga gagaacggaa ttctgcaatc
    43561 atttaattct gcacgaatat atgtagcaaa tgtaattttg catttgattt cgctcgaatc
    43621 acctaatgca aaaaaagtta ttcccattga agaaggcata ttctgtgcag ctaaaccgat
    43681 ttcttcgtca agattttcct cgtccgcggg attcacggag tcacctttat aacttgttgc
    43741 ttcattcgtt atctcgctat taaggtcttc ttcaaaaact tcttcatttt cttcagagac
    43801 agattcttct actctaacac tgtcgtcatt atctgcatta atcatattat tacgcgggaa
    43861 aagaattcct attgaataac gaatatccgg cgaactggta atcaactcat gttcttcgtc
    43921 gggtatcgat atttccgaac ccggacccaa catttcctgt ttaataagtt taacatactc
    43981 gtttcttgct tctattaaca gctttatttc atccgcattt gccatattct tcctccttat
    44041 aagttaatcg gacgatttgt cagattcatc ggtacagaac gctcaacaaa tacaatcgat
    44101 tctaagtaaa gaatcttagc aacagaatca attctgtcat atatataagc ctctaatgta
    44161 ccctctttag gtgttctgcc gcttattttt ttgataattg cttcacctat ccaatccaaa
    44221 atttgtcgtc ttatttttct aatagtttta catgcattga aatagttttg ggcattatca
    44281 aacatttctt catctcctac caataaagca atttgtttaa tggagtcaac ttctcttggt
    44341 cctactgtat gagaatcctc atccagccat cctcgaattg ttgcttcttg gacattaact
    44401 ccgtttctta tcattctttt ggcaatttct cctgccgaga gacctgttat ttgcatatac
    44461 ttctgtaata tttgtttcca cttctttgat tttgaataac tttcaataat ctcagaaccg
    44521 actcttcgtt cgtcaactaa ctttgttaaa acagtatcca caatatctcg tgtttcatcg
    44581 gtattatgcg taaagattaa agaatctcct tctttcaaat cagaaacatt aacttcatta
    44641 acaataccgt cactttcatc aaaaacatat gctttgtaaa actttgtgaa aaagattctc
    44701 tctccgctat caaatatacc ggctgcaatc acatcagcat taggagtata tccagattga
    44761 ttttcaaatg cctcaattcc actgaacgct gctatttcat ttaacttact gatatagtca
    44821 tcaatacttt cctctatttg cgcaacttct tcaacatctt ctacattatc tgtacaatac
    44881 gtttcatctg ttataaactc atctgacata cccgctgatg cagtgttcat agaattcagt
    44941 tctgcttctt cacggtggaa tttccccgct ctatatttat agatattgga ttcaaatgaa
    45001 tagagtattg tttcaatatg tggagatgat gaacacttaa atgcattaaa tcgtttgccg
    45061 acaaaatttc ccatgacaat taccaaatca tatagaacag aattgtcaaa actgttagca
    45121 tttatgatag tcagaaagtg tttattatcc ataatgtcgt aatatccgca ttttgtcata
    45181 acagttacat agtacgtctt cggtacaata attgctatgc gcttatcttt gttcttttcg
    45241 agtaaatcac gaagatattc ttctttttca catttatcac tgagatacag atatgaaatt
    45301 tcaagaagca atattacatt tcttgctctt tcctgcaatt gtttgggtaa agaagctgca
    45361 gcggaatgaa gatctttaag tctttgttta acagaggata tcgtcagtaa tcctaatgaa
    45421 acacaattat ctatttcagt tatcttaaaa gctacagtca taaagagatt catcaagaac
    45481 catgccgtaa taataaaatt ctctttttct tctgtaacat aatccgagtt tttgatgtca
    45541 taaatagctt ccctgaaaat tttatactct tcccaattca tccatccttc aacagaatgt
    45601 gaaattactt gtctgtctaa aactgctgaa acctgttttt caagttcacg ggtatattta
    45661 tttttaattt cacacggaag agtattagac aaaagaaatc cttttgaaca gagaaaaaca
    45721 tttggattct ctacctgtct gattaaagta attgcgtttt ccgaatcaag attcatcata
    45781 acataaatat attgaagtga ttttctgttc aggagttcag gcaattcgga aagactcttt
    45841 tccactgttt catttccgaa aacaattacg ccgacatgtc tatttccctc tcttgatata
    45901 atcaattgtc ttgcaactga tatcttcccg cttaccttta gaatcgcttc ggtttttgcc
    45961 gtatttcccc tatagtgata ttcatcgttt tcggaaaagt aagatgcagt tatcaagtca
    46021 agtaaccgga tgttcttgcc attaaatgat aatgccaatt tatcaatcag aaagtcagcc
    46081 atgcttcgag gcataactaa agcaacagaa gcgttaataa ctgcgggaat atcttctgac
    46141 ttgtagttca aaacatcctt ataaaaatct tctcgaatag tacccttttt gtgcactccg
    46201 cgaccgtcaa gtttttgtga ttttccaaaa taaggttgaa ttttccccca taatttttta
    46261 ggcacataat ccctgctccc attttgagaa attagtatat attcttcttt tttgcctctg
    46321 ttaaaaaagt cttgttttgt atagccttca taggcatatc tcttcccttt ataaagaacc
    46381 aaatcgcctt cttgtaaaga agcaataata tcttctgtat catactcgtt ataaaccaaa
    46441 caagtagctg ccgcatagac aacagcaaca gcattataac aaaccgaacc tgtatgtaaa
    46501 acaaatccgg cattatgttt ttctttattg gttgtttcta ttagaaagtc agcaaatgtc
    46561 tgcatcaata attctcttga aataacattg ccctccaaga gcaaatcgca tctttcaatt
    46621 atttttttga gtgcatttat tgctttcatt ttgttccctt tccgttgaaa aacacattaa
    46681 atgaaacaat acctcttatt ctctgttttt ctaatacatt ccacactaat caattggttt
    46741 aatatctgta aggtccttgc ttctgtacac ccaagcaact ccgccatctc ttgtttactg
    46801 aaagaggttc tccctgcaaa atgatgtacc acatcaaaat acttatacaa agtcttttta
    46861 tgtaatcgtg atgataattt tgcttcaatt tttttgcata gtcgtaattc atcaccatac
    46921 atggaatata aacgtttttc agaatcatca aagttcccct ccattactga taaacaatct
    46981 tttataaata gtgccatttc ttctattacg ggaatagtaa ccgaattccc taactgtttg
    47041 tattgctggg taatcgatat atttctagga aaagaaaatt tttcgtttcc ttctgcgtct
    47101 ttgaaggcat atcctacaaa tccctgtaag cgtccccact cagtaggggt cataacacga
    47161 atgtatttat cattaatggg tgattgcttt ccttttacca ttgtgccagc atattttttt
    47221 ccgttctttt gatcataaat aaggttcctc tctttacctg aacctccagt agccagcaaa
    47281 gtgtttgcaa tcgggttatt aatctccgga gagtttacta ttctgtatcc gaatccgttt
    47341 cctttactct tttgtcttat aatatgattt tcaagagttt ggagatagcc ggaagaaaga
    47401 aaaaatcttg caggaacatc tttttcaagt acttgcttta actgtgaaaa aatagcaggt
    47461 cgctctcttt tcttaggcat ggcatcaggc aatgatgata catgctttcc gaaatatgca
    47521 cgactgaatg caataatata cacccgtggt ctattttggg gtaatccgaa aaacttactg
    47581 ttgcgaataa atgatctgga atatttttct aaattatcat actctccatg cggaaatccg
    47641 actatttcat aatctaattc ttcatcaagt gtcttaataa ttgtcttaaa tgtatttcct
    47701 ttatcatgta ttacaaggtt ttcgacattt tcaaggaaaa caactttagg aattgtgttt
    47761 tttattattt ttgctatctc aaaaaaaact gttcctctcg taatatcctc aaatccgagt
    47821 ttctggccga cactgctaaa tgcctgacat ggaaatccgg ccatcaaaat atcatattgt
    47881 ttttttgtta tttctttttg gaattcttca gatgtcaaat catttttcgg attttcatta
    47941 aataagtgct cataagtaat acaggcaagt tcatcaattt ccgctgatat aatgttttcg
    48001 aagtctcctg caagctcaaa ccctcgtcgt attcccccga ttcccgcaca caaatcaatt
    48061 gtcttataaa gcatttagct ttcctccaag catttaacaa caaaatcagc acaagcagaa
    48121 acattttcct taatatcttt gccccaaaat ctgattactc tccatccttc tgactctaat
    48181 cttttattca cttcagcatc tctttgcata tttctctcaa tcttcggtat ccaaaactct
    48241 cttctcgatt taatttcctc ctttttgttt tcccaatcat aaccgtgcca aaattcactg
    48301 tcgcaaaaca caactacttt cttaccgata aaagcaagat cgggatgtcc gtaaattgtc
    48361 tttacatttt ttctataacg aactccacgt gcccacaatt cctgcctcag tttccgttcg
    48421 atttctgaat ctttgttttt aacggcttgc atattctttc ggcgctgttc cggagtaagt
    48481 ctgtccattc ttttttctat aaatcgagaa atataaaccc ctcttctctt tcctgttctt
    48541 catactctat attatcattt tgtaatgttg ctaatatcac tattaaaagc attataatct
    48601 gtatgtgttc ttgtattgtt tttttgtcaa ggtcgagttt tgttatcgct caaatgttta
    48661 tgcaaaaaac tgaaagtgca tcattttctt gttaatcagc agttcccaca acttattgca
    48721 aaaagtaaat ataactacta attatttatg caatataata acattgtgta tatcataaat
    48781 tttgctatat atcaataaaa ttcataaatt attgatgata tttgattaaa aaagatagaa
    48841 tttaattgaa atgtcccatg gctcatcaat aatttctttg gtcttctttc aagacttgtc
    48901 aatacaaatt ttgattagtg ataactctta aaatcaattt cctttcaatc atagtatgcc
    48961 caacaattac tgaataagtc aattatagca atgtacaaaa tctatatata actattatag
    49021 aaatgtgttt gtgcaaatta tataaacagt ttgaaatttc ttttcaacaa atatcgtatt
    49081 gcataaatca cgaaaaagca acctcgttca attttctttt caaaaaaaac aatattagtg
    49141 aataaataaa cggcaactga aatacttcaa tcaccgtctt ttggtgcgga tgacaggact
    49201 tgaacctgca caatctcgca atcactagaa cctgaatcta gcgcgtctgc caattccgcc
    49261 acatccgctt gctttaccat tttagagcat aatcaccgta tagtcaatga tttaattgtg
    49321 ttgactatat tctcaaatat gcagttttgt gcttgattac tttgtacatt atgttattgt
    49381 ttacatatat caattaagga gtttccgtca tgaatgcaaa gattaaaagt tacaacaaca
    49441 gaccgacaat atttattgat gacaagccgg taccgcctat catttatgct cttaccgact
    49501 ggataggtgg aagattaagt tacgatgaga taaccaggtt ttccataaag aaatttgcag
    49561 ccgcaggtat aaggctgtat cagctggata tttcctttca ggatatgtgg tttgaagacg
    49621 gtacttttga tatctccata gcgttaaagc agattaaagg aataatagat gtccgacccg
    49681 atgcggcagt attcttccga ctgcacatca atcctccgag atggtggtat aaaggcaaag
    49741 aaagtgagtg ggtcagatat gcaaataccg aagtttatcc cgagcctgat atagagttgg
    49801 ccagaaccta tattcttaat gatctgaaac ccgtacccag agcaagcttc gcttcggaaa
    49861 gatggatgaa cgacatgaca atcatggtaa gaagattctt agaggaactc tataaatcac
    49921 ctttatccga gcatcttgca ggaatacagg tagcaaacgg agtgtacgga gaacaccatt
    49981 actgggcttt tatgagaaat gatcccgacc tttctgaacc tatgttaaaa agatacagaa
    50041 agtttaaaaa tgatccgaac gcacaaattc ccggaatgga agagcgatac aatccgaagg
    50101 acggcatatt ctttgacccg gagaaagact ccaatctgat tgattatctt acctgtcagc
    50161 atgaggctgt tgcccaatcc atcattcact tctgtaagct tgttaaggaa acatggaaaa
    50221 gagagataat aaccggcgtt ttctacggtt attatgtttc catgttcgga agagcaggat
    50281 tgggcggtca tcttgcggaa gagatgatac tgcaatgtcc ctatgtggac tttttatcgg
    50341 cacctcaggc atacaacagc aatttaaggc atataggcgg agcaggtgtt gccagaggct
    50401 tgctggaatc ggcaagactc aacaacaaac tgttccttga cgagatggac caccctaccg
    50461 aattaggggt tcttcacgga ggaatgaaag tatatcctcc ttatgaatca ttgcagatac
    50521 taagaagaaa tactttggtt tccttccttt cgggaatggg cttttggtac tatgatttcg
    50581 gaccgtttaa cacatcgggc tggtggtatg aaccgtatta tctggatgag ataaagtcgt
    50641 tacagaagat atacaacaaa tactatgaga aggagtatat aaagagggcg gatgtgcttt
    50701 tggtgtttga taccaaagtc cagaaatata ccgctcttac ggaaaatgcc gaccccatta
    50761 ccgataaagc ctgtataaat gtagcctatc cgatggcatt gagatcggga gcttccatgg
    50821 acaccatata cttaagcgat ttgggaaaaa caaatttgga taagtataaa tgcataatat
    50881 tcatgaacac attcgcactt accgatacgc agaagcagta tatagccaag aaagtttata
    50941 agaaaggccg ttcaatcgta tggttctttg ctcccggata tacagataaa aagagaaatg
    51001 acatagcttt ctgtaagcag gtatcgggat ttgatctgga tgttattgca cctgcgccgc
    51061 agataaccgt acagtgtaaa gacttttcct attccgtaga taacagtgat aaagagagca
    51121 gattgaataa gctgtttgta ccaaaagacg gaaatatatt gggatatata ggcaagactc
    51181 cggcattggc acataaaaca attgacggct gtgatgtata tttctcctca atacccttta
    51241 ccacaccgga atccttcaga tacatctttg aaagaagcgg agtgcatatt tacgatagct
    51301 gtaatgatgc ggttattgaa ggctccaatc ttctattgat acatgccgaa cataaaggag
    51361 agagagttat aaagttcagg gactcggaaa tcaccgtgga tttccaagca ggagaaacca
    51421 tattatttga tattaacacc aaggctatat taagaagagg tgaacaggga cttacggtct
    51481 gattactgtt ttctcaaata catctgtttt acatataccc aaattgttgc taattcgcat
    51541 ttttcgtaat agtaaaacag tattcatatg ctaagcccaa ttcgttcttc tgccctacat
    51601 aacctgcagc ttcctccgca ggagcaaaat actgctttaa atattttatc aactcggtaa
    51661 tgtcggtatc gcttcttgtt tctatagtta ttgcttctat gtgctcgttc ttgaatgctt
    51721 ccttcttata cttatcatta tatcccgtat atgtcagaaa tatcgtgtca tacccgtcag
    51781 gtacagactc acgcatctct atcttgttac cttccttgtc atacacagct ataatttttt
    51841 cctctttttc atccttttct accttatctt ttgtctcttc cggcaattcg taatatccga
    51901 ttatcttgaa tccgacacgc attgcctcaa gaaaattaat gcttcggtcc gactcactca
    51961 ttgtaacaat atggtggtct atacttcgat tattgcgagt agcctcatgc attgctacag
    52021 gtactatggc atatcctttt atatcccctt ccaattcttt ttctttccta tagtcttcat
    52081 ttcttaatgc tgcaagatcc ttactcgtat agttggtata aaatgttatt tcacagcctt
    52141 ttttcattct ttctatttca cttacaccgg taacagcttg atgaaaccac ggccagttct
    52201 taccgctttt tttaaggtaa tcagttattt ctctgccgta agttccggga agtgttatcg
    52261 aaaagctata agacggcacc tttgtaaatg cagggtaatc actcggattc actccttttc
    52321 tgcccgtact tatatttaaa tccgttatat actgtgaata tcgggagtag tcatttatta
    52381 gggcgtcggc gtatacatgc gacatttcca gtttgtcctt cccgtcaatc cgggatattg
    52441 ttattatatt cttctcggag cttttctctc ccgaaagatc aataggcttt aaatccaccg
    52501 tgtttacaaa catatacgaa tacggctcat taaacctgtt tatccttttt tcatactggt
    52561 taagcatatt catatctgcg aaatattcgc ttaagaagta tgagaaattt gtaaggtcgg
    52621 cctgcttatt cacattaatc tccatacaat caatatgatc cggtacatct ttttgagctc
    52681 cgagaaccac cgtacttaat cccgaatagg acagatacaa agtatcactc tcactttttg
    52741 tagtatactc tcctattacc gtgaaatatg ccaagccgct tgtgccgggg tttattataa
    52801 acaacacctt ctcgttatca ggtaattctt cattgatatg actactcaat gtagagggaa
    52861 tgataacata gtgctgaaag ttttccgcca ataaatctgc ggaatatcct tcataaatgg
    52921 ttattttact gtctttttta tcggatatta taccgcttaa tgcggtaata cctttgcagt
    52981 acttaattga acctataacc tcttcgacat tactgctgtc cggatttttc aaaatatcat
    53041 tgtagttata caaacttaag cccttatcgc tcatctcata cctgacggaa gatatgttga
    53101 tactgactat ttcttcttta aatgccaacc ttttatccat tagaactttt gaatatctgt
    53161 acggcatatt aagaggctcc tgagcatccg accttttaaa aacaatgtcg ctgttagaag
    53221 tcgtaacatt gaaacgctct gttttaccga aaagactgtc acggaatatg aacagtaaag
    53281 ctgccgcaac aatcacaacc gatactatga tgattatagt aacacctgtt tttttcttgt
    53341 tctccatctg ttaacctcca ttcgtatatt taaaatatag atgtttatgc aaaccgtatt
    53401 aattactaac taacggtact atataaacta tctcggataa tgctcatact gaaaatatgc
    53461 gaatcaccaa acttgttgcc gttataacaa caatgataca taacactaaa aaccgtattt
    53521 tctttttttc ttctttcatt attaatgcct cttctctttc gtaaaaaatg ataaaactgt
    53581 tttcctgtgt tttttgcagt taatatgtcc cgagtatact ttcaacatgg gcataagtca
    53641 acaaaggagc aaaagaattc atggaaagtt aattttttgt tcacacttta ttaacacttt
    53701 cttcttatac tcatgttgta ttactagcat ttacttcaat aattccttcc tcctctaaaa
    53761 aaatcaaaaa acatgctaat aataccgaaa caatattaac aactcctcat acaaagtttc
    53821 gctccgtaaa atagcaccgt gagtgctatt tttcagtgtc cgcttttctt tgaatcggct
    53881 cataaccaca ccctcatctc cgtttccccc ctgttggcaa agcagtaata gggaatcatt
    53941 ctcaatgttt tctttcggta tttgaacggg gtaaaggtat aaaggtcgtc aatgctttgc
    54001 ggtattttaa tatccacatc cagcatatat gcgtccagtt gatcggactt tactgttttg
    54061 taattgagct ctttcccgaa aactatattg ttaagaggaa aatcgttatc gacagcttcc
    54121 gcacaatata ccacaggacc tctcattacg gcggtcttat gggaattgtc atacactttc
    54181 gggttactgt ataccagtct cggtgtaata tccatttcca atctgattcg gttctgtcct
    54241 tttggcagat tgatgtatat gtaacctttt ttcgaagaat atttgcttct tacatcattg
    54301 attaccaatt ttaaatattt acaccaataa ggttttctta cggcaacttt atttattccg
    54361 gaaactttaa tatatacttt cccgcagttc ggaaactccg tactgatttc tgcccttata
    54421 ttttcatact ccagattgga ggatatgaac tggtttataa acagggtatc cttattatat
    54481 gaatatatgt attcgttaat tgctcctata aatcttatta cattaggagg acagcaggaa
    54541 cagccgaaca cttcctttct ctgagtaacg gcatttctct cggtaagtgt cgtggccaca
    54601 ttcctgttgt tcaaaacggg ctgaatctcc aaaggatttt cataaaagaa cgctttcccg
    54661 tccagagata ctcccgagag cattccgttg taaataatcc tttccaccgt gtccgcatat
    54721 acggagtcgg catcagataa aagcatgcgt tgcgcaaaga atgccaacgc tattgccgca
    54781 catgtttcgg catatccttt ggtattggga agatcgtaat ccaatgtata tgcttcaccc
    54841 aggtacgatg atccggttcc cccggtaata tacattcttt tgttcactat attggaaaaa
    54901 atggctttac atgcagcaag aagttctttg tctttgtatc tgtacgcaat atccgccatt
    54961 cccgaataga gataagatgc tcttactgca tgtccttccg ccgtcctctg ttgccttaca
    55021 ggcagaaaat cctgtgtgtt atatctgttg tagttgctgc ctatccaagg ctcttcttca
    55081 acacctcttc ggtcaataaa gtgcttggaa agttcaagat acttcttttt tccggtcgtg
    55141 tcgtacatct tgacaagagc aagttctatt tcctcatgac ccggtgtttt aaatgcggcg
    55201 gatttttccg ttataaacac tttgtatatg tagtcggcat aatcttccat cagttttaga
    55261 aacttgtcct ttcccgtcgc tttatagtat tgtacggcgg cttccataag atgacctgca
    55321 caataaagtt cgtggtccga acggtgctgc catcgttctt ccggttttct taaaaaatgc
    55381 gagttatagt aaccgtcatc cgacctgttc ttttcaatga gatctactat gcggtctatg
    55441 aatgcctcca gctcgctgtt tctctcttta tgtagtatga aagatacacc ttctatccat
    55501 ttggctacat cggaatcata gaagatgtgt gctatcttct catttgtaaa atcaaacgca
    55561 tcaaacctgc ctgtttccag aaacctgttt tgaacggctt ttaccgtaat atccttattt
    55621 atatcctggc gcttcttcca gaaaccgtcg gttatatcca catgcttggt accgatatgc
    55681 agcatattcg attctccttt gcagctttta tatccttcag taataaatgc taatcctgca
    55741 ttttgaaaac attagctctg agtgtataat caaagtcaaa agcatcattt ttaaccgcca
    55801 ctgctttgag gtagtaagta ccgtccgcaa tatcgataaa attggagata tcggatatct
    55861 tggcaacatg aatatgctga gctcttgagc cgtaattcag aagatccacc tctctgtata
    55921 catagatgtt aataacatcc ttcatcatat acttcgaact cacattaaaa gtaaggctca
    55981 tcttgttcat accgctgctg cttatcttga accagtcggc atcatttgcg gctcttatgg
    56041 tgaaatcaat cttttcagtg ggggttaacg gtgtcgcttt atcccatgtg tcgttattct
    56101 catgcttatc cgattctctg attgtatact ctaagtgtat atcatcaaaa acgggtaaag
    56161 cggaatctat cttgatgtaa tattttcccg ccttgtccag cttatatgcc accctctcat
    56221 catacttgtc ggaggtaaat aacggagcgg catattcttc ataactgtcc aattccgctt
    56281 cgctgtatat tgatacttta gcatatttgt tgaaatccga ttgcattatg taatctatta
    56341 cactgttatc tttttccacg ttgaaataga accagtcggt atcattaact cccgatattg
    56401 taaaggtttt tctttcaccg gccgggaaat aggttgcagt atctctggtg ttgttcagtt
    56461 cgttggcatc ggagttcagc aacgaaagat acaatataca gtcatcggaa ataattgtat
    56521 cggatactat tttaatgtaa taggtaccct tttcctccag catgtatatg tagggagcct
    56581 tggcgctgtt aaaggtgaaa agaggagcgg cttctccgta cgattccaca tcatcctgtc
    56641 tgaaaacaaa tatatttacc gatgctccgg tcttgggaat atatgtgtta agtctcaatg
    56701 cctgattttc tttgtatgtt ttaactgcaa accagtcctg atcattcaat gcgggaagcg
    56761 tgaaacgtgt ttcactgttg cccgatatgg tttttgcact ttgataatca ttgttgggtt
    56821 catatcggtc tgccggaatc acagtataac ttatctctat cggagtagtt attacgctgt
    56881 tatcgacagt gtacagtttg aagtaatatg tgcctgcctc ttccatatac acatacgaag
    56941 ttcccgttcc tcttgtctta tacaaggttt ccaccgcttc acttccggta attaatcgtg
    57001 caccttcata ccagtcgcat acaagttttg ctcccgagaa agatgcatta cgtatgctga
    57061 gcttgaccgt ctgcccgggc ttgtccgtct ttaccattaa ccagtccaca tcgtttgatg
    57121 cggatatggt aacggttttg gcaactcccg gagtaagcat taccgcctgt tcataagaat
    57181 catttccttc caggctgtcg ggagggataa tgtcggttat aagggtgact ttattgagaa
    57241 ccgcttctct tgcggacggt gtcaccttta agtagtatgt ctgcgtctta tcactcttat
    57301 gggtataata tttgttgaat tcgtcggatg tgaacaatac cggtgcggta tccccgttct
    57361 taatcagatc ttcttctctg taaagtgcga catttactcc tttaccggaa ctgatatcat
    57421 tccttattat atattgcaaa gcctgatctt tttgtaagga tatcttaaac cagtctatat
    57481 catttatggc cgagatatct ataatgtacg gtttctttat atctatcggc gttgctcttt
    57541 cccaaacatc attgtcttca tatatatcat ttacgattgt agtgtaattc aatttaatat
    57601 tgctgccgat tgcactgttt cctattaccg aaactttcag ataataagat ccgcctttgc
    57661 ttatcttata tgccgacggt ttgttcagca tgtaggttcc gaacagtctc tccgcattat
    57721 tgccctgact taaaacatcg tctttgtaat acagagccga tataacgccg gttgcagcct
    57781 gatcgtcgca acttatcgtc agaatgacgg atttaccttc cgtcacgggt tccagttcaa
    57841 accacttttc actgaacgaa tcactgaagg ataaggaatg tgtcgtattg ccgtatattt
    57901 ttacggcatt ttcccacggt ccctttatca cttcctcggt tgcagattgt tgaacggtag
    57961 cctgttcaga ctgtactctt tcttcagtgt taactattac ggcatcatca gtaggaaatg
    58021 atgctaatgt tatgacgcta aaagtaagaa ctaataacag aattcctatg cgaactgtat
    58081 tttttttcat tatgtccctc cgaatgatgt ataaaggata catcaatata ctaaaattgt
    58141 aaacacaaaa ggcgccattc cggacatagt tcagaaaaag ttcacaatta accgtaacta
    58201 tcaagtgagt ctttgaagca atgtcttcgg tgtttaaaaa taatacggtt tatgctaaca
    58261 tatcgatagg aggcagcatg agaactgttt tgataaccgg tgcttcaaag gggatcgggc
    58321 gacagacaac tgaggatttt ttggcagccg ggtacaatgt tatcgctaat ttccatacta
    58381 caaaaatgga taaatcggaa tatccctcgt ttcaggagca tatcgaggtg ataaaagccg
    58441 atgtctccga ctatggtcag gttgaagaaa tgttcaaaaa aggcaaagaa cgattcggca
    58501 acattgacgt tgttataaac aatgccgccg taaactatgc gggccttctt tctcagatgg
    58561 aagaaaaaga ctgggacaga attatcaatg tcaatcttaa gggaatattt aacgtatgca
    58621 aatgtgcgat accttccatg gtcaacagaa aagcggggca tattataaat atatcttcca
    58681 tgtggggcat aaccggtgct tcctgtgagg ttgcctattc ggtatccaaa gccggtgtca
    58741 taggctttac aaaagcgctg gcaaaagaac tggctccttc aaatatatat gtcaactgcg
    58801 tcgcacccgg cataatacag accgatatga ataaagccta tactcaacag gaattggaag
    58861 agatgattcc tttgggaaga ataggtaaat gcagggatgt atcggaaatg cttcttttct
    58921 tagccgatga gaagacctcg tttataaccg ggcaggtctt ttcggtaaac gggggaacgg
    58981 tgatttagtt taaggacagt tcataatttc catctattat atgatactat taatgtcggg
    59041 agattaaaat taatgatgag aagaataaga gaattcttta tgaacagata cggaacggac
    59101 cagctgaaca gggctttaat ggttgtgtat gccctcttct tttttgttta cctgataacc
    59161 aaaaataatg tgtttttgtt tattgccctg gctgttgcct tgtatcaggt attccgtact
    59221 ctatcaagga atttcactca aagagcaaag gaaaatcagt ttttcttaaa atactggaat
    59281 cccgttaaaa gaaaatatag ctcaattaaa aagaaagtta ccgataaaac acacagatac
    59341 tataaatgtc ccaaatgcaa aaccgaggtt cgcgtaccta aaaacaaagg caagataaga
    59401 atcacctgcc ccaaatgcag tgagcagttt ataaaaaaga cttaaaataa gcggtttaac
    59461 cgcttcaata ggcgtctacc ttgtatagaa ataattggtg tcataagtta aaatagaatt
    59521 gaaataataa taaattaaat ggggggttgt tatggaattt ctaaaaacat tggaacagct
    59581 gaggactccg ttctttgatc agtttttcct gttagtcaca aaaatgggtg aggaactttt
    59641 cttcaccgta gtaatattga ttgtcatatg gtgtatagat aaaaaaaccg ggtaccggat
    59701 gtttttcata ttcttgctgg gttcctttgt caatcagatg cttaaattaa tctttaaagt
    59761 cccgcgcccc tggacatatg agaatgccgc aaagccggtg gaaggctcgg tggaagcggc
    59821 aaccggctac tcttttcctt cgggacacac ccaacagggc atcggtcttt tcggatcggt
    59881 agccatgttt cttaagaaaa cccgggtgta tatagttatg gtaattctta cacttcttgt
    59941 ggccttttcc agaatgtatc tgggagtcca cactcctgca gatgtattta catctttagc
    60001 tatcggagta gtgcttttat tgctctgcta ctatgctttc cgtgttgcag ataagcataa
    60061 atacggggac gtatatcttt acggtattgt tgcatttgct atcgtaattt cacttatagt
    60121 gctctcgttt gacaaaaccg gctctccgga atatctgcac ggaatagaaa acggatggaa
    60181 acttttaggt tgctgtatcg gtattatagt ctccataatc gtagacatca aatacacaaa
    60241 ctttgaagta aaagccaagt ggtggcagca gattataaag ttggttgtgg gtctcggaat
    60301 cgtattggtt ataaaatcct tcttaaaaca gcctctcctg gacctgttca accaaagtcc
    60361 ggttgccgac ggaataaggt atcttcttat aattctgttt gcaggatgcc tgtatcctat
    60421 gcttttcaaa gtatttaaaa ggaaacagta aaacagacag ctgatgctgt ctgctttttg
    60481 ctatatattc ttttgttaag ttttacttat tccgcttcat actgtttgat gtatatattt
    60541 cccgtagaag tcttaagatt aatgtttgca tctgccgaat gagaaaccaa tacggggatt
    60601 tccgcatcaa atttacctac gcttgtgctg tacgaaacaa caagtttatc ggttttcacc
    60661 gtgtacaggt ttatagatcc ggtatcggtc tccgcatata tactctcggg tggagtagca
    60721 catttcattg tgatacttcc ggtattactt ctgaactcgc ctgtcctgaa ggagccttcg
    60781 gtttccactg tgcctgtgtc ggatttttgt gtaacggact ttactttgta atttttgcat
    60841 attactctgc cgacatccga tttgcagtac agcgtttccg ctattatgtc ttttgcttca
    60901 attcttccgg tatcggtctc cgtattgatt ttttcctccg ctgttatccc gtttaaagat
    60961 atattacccg tatcgctttt aatcacaata ttcttagctt ttaattccga tgcggttatc
    61021 gaagaagtat ccgtaataac agtaatggtg cgacttgcag caagattgct tacattaatc
    61081 cttccggtat gtgtttccag ataaatagta tcagccgtta catcctgtgc tgaaatatcg
    61141 gaaatctgac cgtccgcttt cagtgcggat aatgactcgg ataaggtttt cggaatccat
    61201 ataactactt tcttgggagc tatcctgaat attgactcat cttccagctc actgattttc
    61261 aaaattccgt cttttacggt ataagtaaga ttctctcctt tggacaagtc tctctggcaa
    61321 ctctctataa gcaatatctt gtcctgatta tgtaccttaa attccaccgc tttagttatc
    61381 cagtttacct ctatctcttt tattccggaa gttcccgtct cataccttgc cttttcttca
    61441 tactgccctc tgcgaatctc aatcctgtgc ccgtcgacaa aggaaaaagt aaagcagctg
    61501 aacccgtgtt ccactacata ccatgctccc agacctataa ctacgaatat gattaccagt
    61561 atcgctatgg ttctgattat ttttgatgat gttgcattca tattatttca cctcacttct
    61621 ttgctttccc aagccatata tccaatatac attcgactgc agctccgaac aggaatataa
    61681 gccatgtaag atgccatgca tgtgtctgga aacttattat gaaatacatt atcaggatta
    61741 taagccacaa aaatccagat accgattttc ttatgcttct tttcctctca atttccgcca
    61801 ttctgtcggt ttgagtactg atttgtgtat atcccggttt gttcgtcctg ttgtatattg
    61861 caagtattac acctatggct accaatatta ccgcaatcat aatatttatg agcacactcc
    61921 tgtatgacag cgccattacc accggaaccg ctgcaagtac gaaaatcatt gcaaccaccg
    61981 ctctcaatgc gagatatttc tgcctcttgg aatcatcggg agttatctcc ttttccagtt
    62041 cggcaaaaag ttccgataag tcacctatgc tctccaccgc tatcttatat gcttcttcct
    62101 tgctttttcc ttctgttaca agatcattgt atttatcctt gagattctga agtatttctt
    62161 ctttaacctc ataagccttc gccgtcgcag gcgcatctct aaacagatta ttaacgtacg
    62221 cctttaactt attctccatt tactatcacc cccgtatcaa ttagtttctc aatcatgttc
    62281 tttgacttat tccagtcatt taatagcttt atatattcct cttttcctct ctcggtaatg
    62341 gaatagtatc tgcgccttgc agtcacatta tcttcacccc agtaggaagt aatgaatccg
    62401 ccttcttcca gtcgcttgaa agccgtatac agcgtcgctt ctttgaattc gtattcgccg
    62461 gcggtttttg cagatatatc tttatttatc tcataaccgt aactgtcctt tttcatcaag
    62521 cttgccagta taatcgtatc cgtatttcct cttataagat cagatgctat tgtcacacta
    62581 tctcctcctt tcgttaagta tttcacctta cgtgatacat aataatacaa gatacctcgc
    62641 ctgtcaaggt attttttaaa aattttttat tttttccttt ctcttcattt ttctttttta
    62701 catagttgta aatacaaaaa atttacagtt atatccaata ttaaaaattc gtttcttttg
    62761 ttttttctgt ggataacttg tttttgaatt tatcgcatat ttatcgtata aattttgatt
    62821 ttacctttct tatccacaaa aagcggggtt agcctgtgga taactttgta ttaaaaaaat
    62881 gccttgttta tgggcatttt tatatgtatg aaagttatat ggtttgtttg tccacaagta
    62941 ctgtgagttt tattcttcct cttcctcttg ttctttttcc gttattgttg cggcaacaac
    63001 ggcattatct tcctctattc tcataagggt aactccctgt gtagctctgg ataagatgct
    63061 tatctgtcct acatccagcc taatcatatc tccctgcaaa gacatgagca gaagatcatc
    63121 ctggtccgat gctaccattg aagctataat tgatccggtt cttccagtga ctttgtagtt
    63181 tatgacgcct tttcctgcac gtttttgtat cggatactcg gatacgaagg ttctcttacc
    63241 gaaaccgttc tccgtgacgg aaagaattgt atcttcttcc tttacaagac tcattcctac
    63301 cacatagtcg tcatcatcca accttatccc tataacgcct tttgccgatc ttccggtttc
    63361 tttcgtgtcc tgctccgcaa acttaatagc ttttccttta ttggttatga gcattacggt
    63421 atcatcaccc tcggaaagtc tgacagcaat caaatcatct tcttctctga gagttaccgc
    63481 aataataccg cttcttcgta tattaacata gcttttaagc ggagttttct ttacaaatcc
    63541 gagtttggtg gacataaaca gattctgatt tgtttcaaag ctttcaatag gcagaatcgt
    63601 ggtaactttt tcattgggat ccaattccag aagattgact atggctgttc ctcttgccgt
    63661 tcttcccgct tccggtatct gaaaacctct gagcttatat acttttcctc tgtcggtaaa
    63721 gcagagaacc gtgctgtggg tgcttgtggt aaatatatct acaatattat cttcttctct
    63781 tgtcgtaact cctattattc ccttgccgcc tctgttctgg cttctgtaag tatctacggg
    63841 agtccttttg atatacccga atctcgtaag agtcagcact accgtttctt cttttatgag
    63901 atcttcgtcc agtaagtctt cttcggagtc ttctatagcg gttcttcgct catccccgta
    63961 tttcttctta ataacttcca gttcctccat tacgacctga ttggtaatct cttcatgctc
    64021 cagtatgctg agatagtact ttatcttttc cgtcaactcg tcatactcgg tctgaagttt
    64081 cagtatctcc agtccggtaa gtcttcccag tctcatatcc actattgcct gagcctgtct
    64141 gtcggtaaat tcgaattttt ccaccaaacg tattttggct tccggctcgt tggggctctt
    64201 gcggatggtt tctatcaccg catctaatat atcaacggct tttataaggc cttccagtac
    64261 atgcgccctg gtctgtgctt tatccagatc gaacttggtt cttctcctta ttacatccac
    64321 ctgatgctgt atataatatt ccagcatctg cttgagattc aatgtcttgg gctcatatcc
    64381 ggtcttggaa ggcaccaatg caagcatgtt ggcattaaag gcatcctgaa gttttgtatg
    64441 tttatacagc ctgttaagta taacgttggc attggcatct ttctttaaag taatgattat
    64501 tcttacttct tcgtttctgt ccgactcgtc gtttatttcc gatatgcctt cgatagattt
    64561 gtttttaacc atatcggcaa tagtttctat aagttttgcc ttatttacct ggtaaggaag
    64621 gtcatgaatt attattctct gtcttccgtt tgcaagctct tctatttcgg ctttgcctct
    64681 tactacaatc cttcctcttc ccgtcttata tgcctgatat attccggatc gtcccattat
    64741 ggtcccgtat gtgggaaagt cggggccttt aactatttct atcagttcgt cgattgttat
    64801 gtcgggattt ttaataaccg caaaaatagc gtctattact tccgtcagat tatgagtagg
    64861 tatattggtt gccattccta cggcaatacc gctggaaccg tttacgagaa gattggggaa
    64921 tcttgacggc attacaaccg gttccatttc gtggtcgtca aaattggatt tgaagtccac
    64981 agtctccttg tttatatccg caagcatttc cacagctatc ttggtaagtc tggactcggt
    65041 gtatctcatg gcggcaggcg gatccccgtc acgcgatccg aagttgccgt gtccgtcaat
    65101 caaaggatat ctcaatgaaa aatcctgagc catccgcacc attgcgtcat ataccgcagc
    65161 atcaccgtga ggatgaaatt tacctaaaac ctctcctacg gtagcggcac attttctgta
    65221 agatttatcg ggatagaatc ccaacaaatg cattgcataa agtattcttc tgtgaacggg
    65281 tttgaatccg tcacgtacat cgggcagtgc acggtcggct ataacgctca tagcatattc
    65341 tatgaacgct ttcctcattt gctcttctat atctaccggt atgattttgg aaacatcaca
    65401 caaattttcg ttttccatag tcttttttat gtatctcttt cacaagtttt atctcaataa
    65461 ttataccata aaacacattc ccggtctatg aaaaaaggga agataataag cggctataac
    65521 tccgttttta cggaaaaaac aacttggatt ttaaaaagaa aaagcttgtt taaaaagctt
    65581 tttcgtcgga acattttatt tgtttttttg ctgctctccg tcggttaatc ggtcctgttt
    65641 cttaagaaac tgacaaacga accatccgga ttaaatatct tcagcctcgt tcctctgata
    65701 ttatactgtc ttacatactg ccgtccgttc tcgggattaa agactatata accgttgaca
    65761 ctggtcccct tcagctcata agtccccttc tccgcccatt cgtcaactcc gtacatgaac
    65821 gtctcgtatg tcttatcacc gccgaaatat atatatgctc ctcttgtcgt ggagtcggtc
    65881 tgtatttcca cccaccatgt gcctactata gatttaaata tcaaagccgc cgctataccc
    65941 aaaacaagca ataccaatat ggatatagta actatctttt caatcttcgc tttgcgtatg
    66001 gccttggtct tttcctccgc acttatattc cttctgtctt ccgtcattta taggtctcct
    66061 tgttttcaca ttctatcaca actgtcagta acttgaacag aacttcttta taatcttctt
    66121 gggacagaca tcatagcatt ttccgcatct tgtacatttc tcataatcta tgaccgccac
    66181 attgttgata acatgtattg cgtcatcctc acaggctttt tcgcacaatt tacatgctat
    66241 gcatccgttc ttgcagtttt tgcctgtaac cctgccgctg tcctcgcttg cacatgcaac
    66301 ataatatccc tcttctccta taagatatct tatgaggttt ctggggcagg tctttgcgca
    66361 aagtccgcat tcggtacata tcaccgggtc aataaccggt gcgccgtctt tcatcattat
    66421 ggcaccgttc gggcatgcgg caacgcaatc accgtatccg agacaggagt aaagacagga
    66481 attttcccct ctgtatgcca aagaggcggc tgcacatgtc ataataccgg aataagtata
    66541 tttttttgat acatcgggac aacatatggc tttcattact cttgtttttt gtgtcgccac
    66601 tttgactccc agtatctccc ctatttttat tgccgaggca ttggttcccg gtatgcataa
    66661 attaccctcg gcttttccct ccgcaacggc ttcggcatat aaatcacaac cggaataacc
    66721 gcatgcaccg caattggctc cgggaagtat ttctcttatc gcttccgctt tctcgttctt
    66781 cttcacttca aagaacttac ctgctatagt gagtatcaca ccgaagaaca gagctattcc
    66841 tcctactatg gaaaccggaa tcagtatttc attaagtatc tctttcaccg cttacacctc
    66901 acccgaacat tcccttaaat cccgtaaatg ccatagacag aaatgccgct gttatgagcg
    66961 ctatgggaag acctttaaaa tatttaggca catcattcat atccaatcgc tctcttatac
    67021 cggcaaatat tattgtggct atcataaatc ctaagccccc ggtagctccg tacaaagcgc
    67081 tctgccacag gttatatccg tactgcacat tcaacaaagc cacacctaaa acggcacagt
    67141 tggtggtaat gagaggaaga tatattccta aagccttata taacgaaggc agggcttttt
    67201 tcatgaacat ctctatgaac tgcaccagtc ccgctatcac aagtatgaat gtcaatgttt
    67261 gcagatactc aagattataa gggacaagaa gatatgtgtt ggttaaataa gttacaacgg
    67321 aggccaaagt cattacaaag ataacggcaa ctcccattcc cacagcggaa cttaactttt
    67381 tcgaaactcc caaaaaggga cagattccca gaaagttgga gagaacaaaa ttgttcatca
    67441 ggatagctcc gaaagatata tatataatca ttttaaatgt atctgtgttc atcggctttc
    67501 tcctttctta tcttttgttt cgcattgcac ggggcaatcc gcacagtcct tgatttcggt
    67561 ttttttacgg gtaagagcgc ttaccagtgc cataagcgaa cccagcacca ggaatgctcc
    67621 tgccggtgtt acaaaaattg caggcatagg tatgttcttt ataatcttaa atccgaaaat
    67681 tgcgccgttc cccaaaaact ctcttattgt acctataacc gtcagtgcaa gggtaaaccc
    67741 cagacccatt ccgagaccgt ccaaaaatga ataccaggca ttgttcttgc ctgcaaaagc
    67801 ctcggctctt gccaaaataa tacagtttac aactataagc ggaatgtata ttcccaatgc
    67861 cttgtccagt tccggcagga atgctttcat aagcagctgt actattgtta caaagcctgc
    67921 tattactgtt atatataccg gtattctgac cgtatcgggt atgagctttc tcagaagcga
    67981 tataattata ttggacaaaa caagtaccgc tgtcgctgct atacccatca taaaagagtt
    68041 cttagcagta gtagaaacgg ctaatgtagg gcacattccg agcaccaata tgaatgtggg
    68101 attctctttg attatgcctt ttgaaagttc atgtaaagat ctgtttttca tttaaccacc
    68161 tctgcatgta tggcaagggc acttcccacc gccgaaataa ccgcatcggt ggtaacagtt
    68221 gcccttgtta tatagtctat tttatcagga atattatctt gcattgtaag gccggtaaac
    68281 tgtgaagtga actcgggaag ttccgcgttc ttgcctatgt tgggggtttc atcattgtct
    68341 cctatcacaa tattctcaac ctttccttcg ttatttattc ctacaaatac taccatttct
    68401 cctccgtaac cttttgcaac catggtaaaa acatatcctt tatcgctttt acttactccc
    68461 tttaatttcg ctttggtgtt gatgttcaag ttcatcacct tttcggaaat gtccgtaaaa
    68521 ggcgcaccgc cgggaagcac cgtgttcatt gctttttccg cttcttcttt ctctttggct
    68581 tcaatcggtc ctttggtaaa acgattggta agcgcaagca gtccgacgac acagagggtt
    68641 ataaggaaaa gacttaatac aggagctata tactttttca ttttttctta atcctcccga
    68701 aagttttctt caccgtgaac ttatctatca aaggaaccag cgcattcatg aaaagcaacg
    68761 aaaatgcaac tccttcggta ttggaaccga acttccttat acttgcggtc attaaaccta
    68821 acccggctgc ataaacaatt tttcctttac gtgtcacggg actggtagtg tagtcggttg
    68881 ccatatatat ggaacccaac atcattcccc ccgcaagtat gctgtaaagc ggatcctgtc
    68941 cgaaagcaaa actcaataaa gcgaccgttc cgatatatat cacgggaatt tcccatgtta
    69001 ttacttttct tatcataaga tatatgccgc ccgctatcag tgttaccgct gcggtctccc
    69061 ccaagcttcc gggagtcttg cccaggaaaa ggtcaagata ggaaggcaac aactctcctt
    69121 taggtatagc caaaggtgtt gccgccgtca ctccgtcggt caaaggtgcc acatataccg
    69181 tcattaaaga ggaaaaggca gttgttaaga aaattcttgc ggcagcagcc gggttgaata
    69241 tgttttgtcc tattcctccg aaaagcattt ttattaccac tatggcgata aatgcgccta
    69301 taatgcttaa aataattgaa gacttcaaag gaggcatatt cattgccaga atcaggccgg
    69361 taacaacaca ggaaaggtcg gtctcggaat gtttcttttt tgttataaga ttgaaaagat
    69421 actcaaacag aacgcatgat gaaaccgagg caactataat aagtgcggaa acataggaaa
    69481 agtaatatac cgataatatg gcagtgggca ttaacgcaat tatcacatcc agcattatcc
    69541 ttgatgtggt ggctttgtcc tttatatgtg gtgaagaaga tactattaat ttatccattt
    69601 ttcaccttcg ctttcctcac aagatctttt ccgacacgga aagactgtac aagatgtcgc
    69661 tttgccggac agacatatga acaggatccg cattcaatgc agttcattat attatatttc
    69721 tccaactcct cggtcatttt agccaaggcg tatctgttca ttgtcagggg cataagaccc
    69781 atgggacaag cctctacgca acgaccgcat cttatacatg ccgattcttc cataaaatcc
    69841 agatcttccg gagtcataaa cagaaatgcg ttactttgct tgacaaccgg catctcatag
    69901 ctctccgccg ttgtccccat catggggccg ccgcaaagca ccttgaccgt tttttcttta
    69961 tcaccgttac aatattcaaa aatatctttt atgtatgttc ctatgacgac ctgataattc
    70021 cctctattct taactccgtt gccttcgacg gtgattcttt tggaaaccag aggcattccg
    70081 gtcttgcagt acttatataa tgccagcacg ctggttacat tcattaccac tgtccctatg
    70141 tctatgggca gaccgttcat aggtacttct tttccggtta aggcataaat gagcattttt
    70201 tccgcacctt gcggatattt tgtcttaagc ataaccgttt ctattctgca atcgtctttc
    70261 gcaagctcct taagcagaga aaatgccgca ggtttattgt cttccactgc aatatatgct
    70321 ttgggaatgg acagtttatc taaaataagt ttaactccgt ttactatttc attgccttct
    70381 tctattattg ctctgtagtc acaggttatg tacggttcac attccaccgc atttacaatg
    70441 agagcttccg gaattctttc ctttgtgtcc agttttacat gagcgggaaa tccggctcct
    70501 ccgagaccgg tcagcccgct gtttcgaacg gctttaagaa atttttcctt tgtcgaaata
    70561 tccggttggg ttatacccgg taaaacttcc tgcttcccgt ccggctcaat aactatgctt
    70621 tcgcatttta caccgctatg aagaatcata tcctttatct gcacaacagt acccgatata
    70681 cttgaatgta tcggagccga tacgaacttc tccgtatctc ctaaaacttg tcccacataa
    70741 acataatctc ctttaccgac aagcactttg cagggtgctc ctatatgctg ttgcatgggg
    70801 atttcaacaa gcttcgggat tggaagagtt tccgtaggaa tctccgaagt tccttttctg
    70861 tgaggtaagg taactccttt tatgtttttg atttttatta tcatccactc cctctcaaat
    70921 tataatcatt gatattatac accgctacag gtagaaaaaa aaggacattg tatgcccttt
    70981 cttttatcct gatataatat cgtttattcc gttgccagga acaaaacata ttccgctatt
    71041 gtgtttgcat actcttcata tgtcattgac gcatcttccg tgaatgacat atagacctgc
    71101 attctctctg aaagacatcc ttttatttca ttaagactca taggaacttc ctgctgttcg
    71161 gtatcatcgg gagctttgta aagaacgcgt aatgtaagat tggtgacatc atcgccgtcg
    71221 aaaacgaaac tgacattgtc cgtggccata tattcctttg ttatttcctt tgctcctact
    71281 gcagttcttg cattattgaa gaatatcatg aattcctccc atgcatcggt ttcctggttg
    71341 gttcctaaaa gtgcggggaa attcatcagc agtgttccgt caatttccga accgataatc
    71401 atatttacac ctttgtttgt taccgtcgtt tcaaaaccgg taaagtattc acaggaaaac
    71461 agatagtggt tcatttgtct tatctcgtat gtctggtaca gttgctcatt tctgtcctgt
    71521 acgggatctc ttccgatcat ttcattaaaa gcactcagcc ttgcattcag tgtcttctgt
    71581 ctgctgatgt tgttgaactg tgaaacggta ggcttcatga tagggtctcc gccgtacgtg
    71641 gcaatatcat aaggaaactc tttaatgata tatttgtctt ctaaagcatt tgcggtattg
    71701 atagacggtc tgggtctttt atgatacggg gtacatgagg taagccccac tacaaatcct
    71761 attgcaagaa ctattgctat tactataact gtttttcttc tcatttaatc ctcccatgcc
    71821 tttgaacagc actattttat gaaataattg gtacttatat tatacaactt aagattttga
    71881 ttgtaaatca gttttcacaa ttgcatcata ttgtttatgt gatatttaat aggaaaatta
    71941 taataaccgt taaaatttac tcaattattt ctaatactgt ataaagatta tattcttttt
    72001 cacagacagg aaaaggacat tttctgtcct ttccttctat tattgaaata atactattct
    72061 tgttgtaaga cgtattccag aattgtattt gcgtattctt cataatccat taagggatct
    72121 tccgtaaagg acataaatat ctgtattttc tccaaaaagc accctttaat atcattgagc
    72181 ttcatgggaa cttcctgctg ttttgtatca tcgggagctt tataaaggat acgcaatgta
    72241 agatcggtaa gatcgtcgcc gtcaaaaacg taactgacat tgtcggtagc catatattcc
    72301 ttcgttattt cctttgcgcc cgctgctttt ctcgcttctt caaacagcac catgaattcc
    72361 gtccacgaat cggattcctg ctttgttcct aaaagtgcag ggaaattcat caaaagcgtt
    72421 ccgttgattt ccgaacctac aagcatatta acacctttgt tcgtaaccgt cgtttcaaag
    72481 ccggtgaaat attcacagga aaagagatag cggttaattt gtcttatctc atatgactgg
    72541 tatagttgtt catttctgtc ttgtccggaa tctctgccga ccatttcatt gaaagcattc
    72601 agccttgcat tcagtgtttt ctgcctgctt atgttgttaa actgagcaac ggtaggcttc
    72661 attatcggtt ctccgccata agtagcaaca ttatattccg cttccacaac tatatacttg
    72721 tcttccagag catttgcggt gttgatagaa ggagaaatcc ttatttcatg cggggtacat
    72781 gacgcaagcc ccgttacaaa tactattgca agaattactg ctataagcat aactgttttt
    72841 cttttcatat tcacttttcc tttcttagct gtttttgaga aataacaggc gcccgcatta
    72901 cttcttatgc tttgattgta aatcggtttt tataaatgca caataatgta aatgtgacat
    72961 ttaataggaa aattataata aatgtcgaga aattcttgaa aatatatgca atataataga
    73021 ctttctgtcg gaatcttcaa aagaaaggga acaatatggt gctgtattga tgattgttat
    73081 atgttttttc cgacaattct attataaatt acacattaat ctcttaaaat gcttaaatgt
    73141 gacattttat gcgactttta taactctttt ctcttaaatt gccttttcat atatttcggt
    73201 ccgacttaat ctctgtaaga aaaaagacac acaatgttgc atgtcttact gtctggcgcg
    73261 cccgagagaa ttcgaatccc cagccttttg atccgtagtc aaacgctcta tccagttgag
    73321 ctacaggcgc atgtagatgt atattaatac tttttataca ttttttcaag gtgttctccg
    73381 ctttcaattt acttgccgag cttttttata tataattatc agcaagaagg acgtaatcat
    73441 gaaagacaaa tttgacagca aatccatatt gtattccgac acattaattc ccgacatttt
    73501 cttatctgaa tatcttccgc tgctttcacc gcaggcagtg aaggtataca cttactgctg
    73561 ttttttggag aaaaccgaaa gaaccgtcga tattattaaa ttgtccagac gtctggatgt
    73621 tgaagaaacg gcacttacgt ccgctctgga agaacttaca aagaaacatc ttctatccgt
    73681 cagtgacaaa gacatttttg taaatgacat taagggaata gagatagacc gtctttacaa
    73741 agaaagaact tctttaaagc ctgatgatct gggagaaaaa gcgtctgtaa tatccgccat
    73801 taacgaccag ttctttgacg gtaatatgcc tatatatatg tacggatgta tagagcaatg
    73861 gtttaaaaaa tacagatttg aagatcctgt catggttatg cttttttccg taagcaacga
    73921 aaagggtgct ttgacccgta actacattga agcggttgct aaagactggt ttgaaaacgg
    73981 agtaaaaacg gtttttgatg tggaagcatt atttgaggaa cgggacaaga tgaaagatat
    74041 gcacacaaga attcaaaaag ctttgaacag aaaggccccg ttcacgcaat atgaaacgga
    74101 ccttatcaac aagtggttta atgagtatca ctacagtttt gaaattgtcg aagaagcgtt
    74161 gaagaagact gttaaaatat ccaatcccaa tatagcatat gtggacaaga tactttccac
    74221 ttggtatgaa aaccaattca agaatcttga tgatcttgag aaggacaaat ccatcagaga
    74281 cctttctccc gaccagctgc gtatgattat tcaagaacac taccaaggca taaatatgaa
    74341 gaattccatg ctttttgaaa gccgtaaagc ggaagtattc aaaaaatctc ctcagattga
    74401 aaaactgtac aatgaaataa acgatttaca tttcaaacag gctttttctc cggacaagaa
    74461 agccgttgca gaagagataa agaataagaa ttatgaaatg tcgctgctgt tcaagcacaa
    74521 caatattccg gaagattatc ttataagaaa atatgattgt gatatatgca aagacacggg
    74581 tgttgataac ggcaaagact gcagttgtaa aacggatttt ttacgtaatt acggaacgaa
    74641 atagttttct atttgcttaa ttgaataacc gctatatctt tcccttccaa aaggaaattt
    74701 tccatatcgg ttgcgaaaaa cagtatctga ttatgtctgc ttagctcgta taggaaatca
    74761 aaagtactct tctttctttc ttcatcataa tatacaagtg tctcatccat aaaaacaggg
    74821 gttacgcatt cggaagaaaa aacctttgca gcagccagcc ggaaagccag atacatctgt
    74881 cttaacgttc cgtcggagaa actgtatacg gagcgtattg ttttattatg ttcggtattt
    74941 aggtccattg tgctgccggt tgataagttg tcatgctttc ctgccgttat cttttcaaga
    75001 tactcactca tgtttttatt catttcggga attatttctt ctcttatctg cttggacgcc
    75061 tcctctatcc cgtctattgc acattgaagc gccttctgtt tttccccaac cgctttcttt
    75121 tgctcttcca gtaataatat atgctctttt tgtgatgcta tctgctcgtc ggagttcata
    75181 tatgcatcta attgagcttt gactactccc aacctgacct ttttagtctg aatacgatta
    75241 aggcactctt cataatccat agaaggttct ttcccttcgg ggttgtatat atcggctttt
    75301 tccaatagag cattatagtc cgatacgtcc tctttgcgta aaatatcttc ctgtaatccc
    75361 gtatatcctt ttatcaggtc attaaaggat ttgtcatttc ccaatactgt ttcaatttgc
    75421 tctaactcat tctttgtttt tataatatca tccaactttt ccttttcatt aaccgtttcc
    75481 ttattctttt tacgagtaat gagaagcagt acgataaacg gtattgcggc taaagcggac
    75541 aacagtaaca atttgtccgt tgttattgcc aacacggcgc ctgttataat aatcagaatt
    75601 gcaagtattg aaaaagcggt aatttttgta ttatttctct tttcctcttt cacaaatttt
    75661 tcatatgctt tttccggctc tttatattcc ccggaaacgg tatcaaattc ctttcttctc
    75721 ttttcataag catcaatatc tgtatcggaa agtttctttt tcagcggttc tatttcgtta
    75781 ttgtatcctt catattcctc aatgttttca ttgacgcgtt taaaagtctc acttttcctg
    75841 cagagttttt cctctttctc cagttcttca agttcttttt ttatctcttc cgcttcggcc
    75901 agatactcca tgcatttttt gttctcattt tgcatgtttg taagtttaat ttcctcttcc
    75961 cttatctgtg cactcaatat gttatatttt cttccttttg ttctttcatt acctaataaa
    76021 gtaatgcctt ctttaagttt ctttacggct tttccgacag atatctgttc ttcccccgat
    76081 tcgtttatat tcataagttt ctgtgctata tgttctttat cttctgcgta taaaaggctt
    76141 ttgtcctgac atataaatga cgaatttgta aagcactcat aatccatgcc taagagatcc
    76201 tgtcccagca tctcgttttc tttactgaag gaaaattcgt ttgttatatc ttcaccgtat
    76261 tcattgtata cggacagagt ttttccgttg aagtctcttt ctactcttaa tattttcttt
    76321 tcatcggtct ccagtataag atatccgcca tatttgtctg aattccacgg tttgtacttt
    76381 ttgacttccg gaataaatcc gtccttttct ctttcgttct tcttatgccc gtaaagcatc
    76441 gcttttataa acgacatcag tgtacttttg ccggactcgt ttttccccaa cactaaattc
    76501 agaccttctt tgaaattgaa tgtcctgtcc gccagtttgc cgtatccgta tatgtgagcc
    76561 gctataattc tcattccaaa tcaagatcct catccgataa agcctgcaat cccagcttta
    76621 aagcatcttc atatatcttt atctcttccg aaggaatatt ttccatcttg cttgtcatta
    76681 tatcggtaaa tctgcctttt attccccgtg taatctcttg agcttttttt tctttctttg
    76741 cagtccttgc atctgtcagt tgtacatagt catatccttc gggtattgca agcggcttat
    76801 tgtcgctctt tgtccctgtc acctttattc tgtatatgtt ccgtttgtcg ggatacatgc
    76861 tttcaaagtc tgcaagtttt tcataatcca tctttaaatc aatgtatttt ctggatttac
    76921 tatttacaaa ctcaacctct cccgtagaca aatctccttt gaagtaaccg tgggctcctt
    76981 cttctttaaa agacaatgca caaaggcttc ccggattaaa tatattgtct tttatataca
    77041 ttctgtgatt atgccccaat gccacataat caaaccctat atctttcaac atatccgaac
    77101 tgaccgcatt gtatgtggaa agagacaaat ccacatcgcc atgcaaaagc agcacatttt
    77161 tatactttct gtcggtacga attgtctcca aaatttttgc ctgaccgcct ccggtatcat
    77221 agcctattcc gtagacacac actttctctt tttcaaaaat aacattcggc ttgtttcttc
    77281 cgagcacatg tacattttcc gaccacggga agtggctgta aaaagagttc ttggattcgg
    77341 ggtcatgatt gccggatata aggagaatgt ttcctttaag tttggaaaag gcttcattaa
    77401 tcttacttat tctttttata tttgtatatc cgtgttcata aagatctccg gcaataaata
    77461 ggaaagcagg ttgttcttca cgtgccgttt ccagcactct cataaactca tggaaaattt
    77521 cttctcttct ttttgcaggg aagccttcaa ccaatcctaa ggatgaaaac ggctggtcaa
    77581 gatgtatgtc agccatatga aagaatgtga gaaccatctt gctcctcttt gaatgttatt
    77641 accgcatacg caaccgaata ttccttgcaa tgtgagagac ttacacttat gtccataata
    77701 tcggggaaac gagctttcag ttgctcattc aacaggatat gcggtttccc tttctcgtca
    77761 tttataactt caatatgaat gggttgtacc gtagcacaaa accccgtccc caatgctttt
    77821 gacaccgctt cctttgcggc aaatcttgca gcaaggcttt gattcatcaa agtttttttg
    77881 gatctgcagt attttatctc tcccggtgta aagttctttt ccaaaaacct gtctgaacga
    77941 cttacggcat cggatattct tttattctcc actatatccg caccgcatgc aatcctcatt
    78001 taagacctcc cgatgtcttg gttatgaata tgtcatcatg ttttcttgac aaagacgtat
    78061 atgccacgct tgcaagtctc atttcgctgt acattgccgc tacagcggaa cctgaaccgg
    78121 tcataaacac cgtaacggca tctgtttcgg aaaggtcatc cattactttt tgaatggaag
    78181 gcttatgcgg tataacaact ttttcaaaaa tgtttacaac attacccttt agtccttcaa
    78241 tatctctttc ttccagcaaa gactcaatca ggtccatatc cggtctcacg ggattgcctg
    78301 ccttgtcata ttctttatat gcccatgccg tctccacgga atcttcgggt tttattatca
    78361 cgaaccatac atcggaaaaa tctttcaaag gttttaatac ctcgcctctg ccggttgctc
    78421 tctgtgttcc tcctcttatg caaaaaggca catcggaacc tatcatcgca gccatttttt
    78481 ccaaatcctc atatggaaga tttgtattga aaaggaagtt cagtcctctt aataccgcag
    78541 cggcatcggc acttcctccg cccagaccgg aaaacagagg tatggactta tacagatcaa
    78601 tttttatgcc gcgctttata cccatctctt tcatgaaaag ttttgcggca atgaaggcgg
    78661 tgttttcatt gttcttaatc gaactgtcat cacaccagaa ttgtatccct ttctctcttt
    78721 cggcaaaagt tatccggtct cccaattcca ccgtctgcat gacggtatca atttcatggt
    78781 atctgtcctg tctcaaccct ttaatatcca tgcctatatt aatttttgca tgtgccttct
    78841 gtacaatcat cgctaaaaat ccagtactac ttttaccgcc ttgctctcag acatcagtct
    78901 gaatccttca ttaatgtctt ccataggaag ctcatgggtt ataacatgat caatgaacga
    78961 ggagtttcct ttaagaaaat tctggcattg gtaccaggta tcaaacattc tccttccgtt
    79021 aattcctctc aatgttatac ccttgaaaat aacatacttg cccatattga tattcagttc
    79081 ttccgacgga attcccaata tggccatatc tccgccgttt tctattacct ccagtcccaa
    79141 ctggaatgcc gacggaaaac cgctcatttc cagaacaact tccggtcggt taccgcggaa
    79201 agcttctatt acctgttgct cgggattatc ggataaagga ttaataacca catcggctcc
    79261 cgcctttttt gcaagtgcgg ctttatacgg ttgaggttcg ctgacaacca gtcttgccgc
    79321 tccgcatttt cttgctatgg ccgttgcaaa caaccctatg gaaccgccac cggttattaa
    79381 gacatccctt gacgatatgg gttgtgccat taccgtatgc atggcattac ccagaggatc
    79441 cattatagct ccgtatttgt cggcaacctc gtcgtccaag ggccatacat ttattgcagg
    79501 catttttaca tattccgcaa aacaaccgtc tatatccact ccgattatat ctacatcttc
    79561 gcatatatgg cctcttccgg ttctgcagag tttgcatctc tgacaggtaa tatgtccttc
    79621 agcagacact ctttgtccta tatataaacc ttttaccgat ttacctgtct ccactatctc
    79681 tccggcaaac tcatggccga ttataagcgg aggcttaatc ctgttttgag accacttgtc
    79741 ccatttatat atgtgaaaat ccgtcccgca gattcccgct tttttaattt ttataagcac
    79801 ttcgttttca ttgatttcgg gaaccggcct cttcgcaagt tccacatttc ccattcctgc
    79861 cgctgccttg ataatcgctt tcatatttac tccttaataa cattttaatt ttatcatatc
    79921 ctaacccgtt tgttaacgga tttaacatat atccgcattt atacggtttc ggttttgttg
    79981 gcatagctcc gcattttact gaataatccg tgttttttat agtataatga ccgtatgaaa
    80041 aacttattcg acaaactgat gcaaaacaga cttttcagag aattattcct tttattcaaa
    80101 tacggtattg tgggtattgt aaatacaggt ttaacaacgg gaatattctt tttgctcagt
    80161 catctggggc ttaaatatta tgtttacacc gttataggat atatagtagg catacttaca
    80221 agtttctttt taaacagaaa atatactttt aaaaaagata atgaaaatat cacccgacaa
    80281 attattctct tctttgtaat aagtgcctgc ctgttaggtc ttgttcaact tatacagtac
    80341 cttcttattg atgtattgtc gcttaaggaa tggctgggtg tgggtatcgg gatggttttc
    80401 tataccgtta ccggttttct actcaaccgg aatgttgttt ttaagtataa agataaagag
    80461 aaccccgaac aataaaactt ctcaggttct tttgtattat cttttaacag ctttccttat
    80521 ataacttccc aacctctgaa ttcgtttggg aatattagtt ttatagttgt tccctctccc
    80581 ggtttgcttt ccacttcaat ggctccgtta tgaaggtcaa ttattctttt gacaagagct
    80641 aaacccaatc ccgttccttc ttttgaatgt acggtgtctc ctttgaagaa tttttcaaac
    80701 attctgtttt tggtatattc gtccattcct ataccttcat cctgtattac tattacggct
    80761 ttttcttctt cattgtacat ctttatatga acaactcctt tgggatggga gtaagtaatg
    80821 gcattgttaa aaagatttat ccatacctgt tgcatcagat cttcattggc ataaaaggat
    80881 atatgttcca tctcaatgtc aaaactgata cttttttcat accatttagg ttctaagagt
    80941 aatataacat ttcgaatctg ctcatcaagg ctgtattcgg acttgttgaa tactatctcc
    81001 tgactctcca gtttggacag attcaatata ttggtggaga gattggaaag ccttttggat
    81061 tcctcgacta ttatatccag atattccttt ttttcttcat ctgtgagatt agggctttcc
    81121 agaagttttg caaatcccag tattgatgat atgggagttt tgaactcatg tgatacgttg
    81181 cttacaaaat cctttctcag atactctatg cttttaagct cctttgccat cttgttgaaa
    81241 ctttctgtca gctgtcctat ctcgtcctga gaatcattta ctacaactac atcaaaatta
    81301 cccttggcca cttcctgcgt agcattatta agttgcacta taggtttcat tgctttgttg
    81361 cttaatccca tgattatcag catgaacaca acaatggaaa acagaaacgc ataagcaagc
    81421 aacatggcat agtttctgaa caggttgttc tccggctgat atcccactct taagtaatat
    81481 tcatcggttt tcacgacgga tattcctacc ggtattactc tttgttttat gaagacaata
    81541 attccgtttt ccagtaacgc tttttccgtg tcacttaagc gaagatcatc tatgcctttg
    81601 gtgagtttaa caggataaag ggcattatga gttatggcta taatcctttc caaaggaagg
    81661 tcggtctcct tatttgccga caggatggtt tcagctacat atctttggtt ttcctttatg
    81721 gtttctataa agtttttgtt ggacaccaga attgctccca tatatgcaaa cacggaagct
    81781 actacaatga taacgagcag gaatatggat aatttagatt tcagttgcat ttttcttttc
    81841 cgctttgtaa cccagccccc ttacggtgac tatttcaaat tcatctatat tcacaagttt
    81901 ttctctcagt ctttttatat ggacatctac tgttctttcg tccgtttcac tgtccaatcc
    81961 ccatatatca tccatcaatt gctgacgcgt gaatatcttt ttagggtaac tcatcaattt
    82021 aaaaagcagt agaaactctt ttttgggaag aactatcctt tcggtatctt tctgtacgct
    82081 taacgtgtca taatcaatga tcacatctcc aacctctatt ctgtgttcgg tagctatttt
    82141 agaccttctt aaaagagcgt taaccctcag cagcatttca tcggtgttaa tgggttttac
    82201 catatagtcg tccgcaccgg acagaaaccc tttcctcttg tcctccagtg tttctttggc
    82261 ggttatcatg agaatgggca tattgtaatt ggaccacctt aaactttccg tcagctcata
    82321 tccgtccata ttgggcatca ttatgtcaca tatgataaga tcggcttttg tgctgtcaag
    82381 aacctccaaa gcctgttctc cgtcattggc gcagagtaca ttaaaaccgt tctgtttaag
    82441 aaccgtccct ataagttttc ttaagttttt atcgtcttcg actattaata tattaaacat
    82501 aaataatctc tctccgatac atattatata tcaacaccgc cgtgatataa attgtataca
    82561 caccggaaaa agaaataatt tataaaaata tctgttgttc agtcggtcat taagctgcgt
    82621 aatcccgcta ttctgtctac gggaacggaa aataccgctg cattggcatc ggtgcctatt
    82681 cctgcttttg tcatgatggc gctcatgatt tcatcttttt cctgcttctt tgcaaggata
    82741 tatatcattt ccttttcttc cgctatggaa acaccgaaaa atctttcatt tgcattcact
    82801 tccgtctctt ttgccgatac tacggtaccg ccttttgcat ttgcactacg cgcagcttcc
    82861 attaccgttt ccgatgctcc tttgtctgct attacaatta ttaatgagta ttttaagttt
    82921 tccatcttct ctcccttttt tttgcctatt tcctgtcctt cagtcagata ctttaaacaa
    82981 gattctcctc cgatactttc caaaggtatt gtaagtgcta taccgttacc ggcgacttca
    83041 atttccatct ggtttacaag attttttaat attatttctc tacgatcatt atctacgaag
    83101 atatgaaaca ttattttctc tgttttttct attccgagat aatccagcat tttcttttgt
    83161 gcggttcctc tgcataaatc ggacaatacc atgtgtacat cgttttccct gaagaaatta
    83221 aggtatgcct cttcaaactc tcttttcgta attattatca tatagaatat gttgctcatt
    83281 tcacacctgc catcaaaaat tgatgatgct ttcctttttc tttattttct gttgctgctc
    83341 ctgttctctt tccgcagcct ttaacttgaa ccggtagaac agtccgagaa cctgtatcgt
    83401 tataaacggc gtcattgtaa ccaatgctat tattccgaaa gcatccacta ttacattacc
    83461 gcctacactg agacatacac ctattgcaaa aggtaccaga aaagttgagg ccataggccc
    83521 ggatgagaca gcgcccgagt caatagctat tgaagtaaag acgggcggta cgaaaaatgt
    83581 caatatcaac ccaataacat atccgggtat aacaaagtat aaaattgacg cacttgttaa
    83641 aatcttaatc atgcaaagac ctatcgatgt ggctaccccg actgaaagac tggtggcaat
    83701 tgctttcttg ggtattgctc ccgatgacaa ctccacaacc tgtttgctta acacatgaac
    83761 cgccggttcg gaaataacca ggaagtatcc tattaccatg ccgatgggaa tgacaatcca
    83821 attgtacggc agactcccca gcgcttttcc taaaaaactt cccatctgta gaaagcctac
    83881 attggctccg gtaagaaaca aaaccagccc aacatatgcg tatataagac ctactacaat
    83941 tctgataacc tgtccttttt tgagctttaa aaccgccagt tggaaaatga gaaaacatac
    84001 tacaataggc aatattgcca atgctacttc acccaatttt cccggtatct ccgttatgaa
    84061 caagcgggtt atctgtgttg ttttgccggg attaaccgga gcggttgctt catatgtcgt
    84121 gttgccgggt ttatataaaa tacctaacaa caatacggct aatataggtc cggccgcact
    84181 taatcctaca aatccgaaac tgtcgctatg tgcatcttta tcgcttctta ccgcagctgc
    84241 tcccgcaccg aaagccaaaa gaaacggaac ggtcatggct ccggtggaag ctcctcccga
    84301 atcaaaagca agcgacagga attctttagg agcaaaaaac gataatgtaa atattaccgc
    84361 atacactccc atcagaacat atgccaattt aattctgaat ataactctta acatggagaa
    84421 ggttaaaaac aatcccactc cgagcgatac cgtcaatatc aaaaccatat ccggtattcc
    84481 gggtacctgc tgtgccaata cggtaagact gggttccgat acggttaccg cagttccgat
    84541 aataaggctt gcaataataa tcaaccatag ttttcgtgac tttgttattg atgaaccgac
    84601 actttctccg ataggcatca tggcgatatc ggcacctaac gtaaacagcc ccattccgag
    84661 aataagcatt acggcaccga gaataaaagt cagcattatt ccgctatcgg ccgggaaaaa
    84721 gataaagcaa agtataaaca caactatggt taccggtaaa accgaagaaa gtgattcctt
    84781 caatttttca agcagtatag ttctgttgtg ttttataaat tccctgtttt ctctgctcat
    84841 tacatgcctt tccaatcaat ggataataat ataccataat atatgggaat ttgtaaatta
    84901 attatggaaa ataaaacatg ttatacaaga ctaactaaat taaacaggct tagaagataa
    84961 ctcgattatt ttgtccatgt tgtcgtcttt tgcaacaata ttcttcttaa cagctccgca
    85021 tctttgacac ttgaatacta ttttataact atccctgtac ttctccaagc ctataggttc
    85081 cattaagcca tgacaatcac acatgcgatc tccgggattg ttgtccacat gtttggaata
    85141 cagacaacgg ggacaatgat ttcttgcagt aactccgaga gcatgtactt ttactccgca
    85201 tgcttcacaa acaaatgact catctttcat tgtaaattta cgagaatcca tattttctcc
    85261 catattcacc atcgcctttc ctctctgtct ttctaagcga aagaaaggaa aaaccgaagc
    85321 gatatatcaa aaagacacct ttcagtcgtg aaacgatgtc ctttatggcg gagagacagg
    85381 gattcgaacc ctggttacta ttcctagtaa acacgatttc cagtcgtgcg ccttcgacca
    85441 gctcagccat ctctccaaac gcaagtcata ttttatacat cgtctcataa gatgtcaatg
    85501 taatattgct ttgtaagtat ttgtgcgcac ttttccaata gtgctttatc atatttatta
    85561 aggtgtgtca gaatggtgta ctttgaagaa ctcattttgt atccgtataa gatactgtca
    85621 tggaaaatat cctctggaat acctaaagtc ttcggatttg tttcacaacc ggcactcttt
    85681 aaaatattct caatgttctc agtattcaag tccgttactt caacgggaag tttttcctta
    85741 attaaatcat acagtctgca aatcacacat aatgccacac ctacggcatt tccgtgtaaa
    85801 ggatgaaccc atcctctttt tataaactca atatcccaat aatgcacaat gtgatgttca
    85861 catcccgaac ccggccttgt atttttgtac atgcccattg ctataccgga aagaattagt
    85921 gcttccatta taccggttac cgccttttta tctctattct tcaagagaga ggcgctgtcg
    85981 gcgcatttgt ccgcagcctt ccaaaccatt tccgcaattt cttcacagta atgttccttg
    86041 tttaagtctc tggaaaatct ccattctctt accgcattgt atttagacaa tacatctccg
    86101 aatccggcag taagcatggt atatggagca ttttgcagta tatctatgtc cgcaaaaata
    86161 cctttcggta cctgtccggg acagctttct tttacgttat ttattattaa cgacgatgtg
    86221 attgaagcat atccgtccat ggaaggagcg gttcctatta ccgcatattc ctttcctaaa
    86281 tgataagaaa tatttcttac aacatcatta acaactccgc ttcctactgc tacaaaaaaa
    86341 tctattccca aaggcatcct tatcaaagtc tctcctatgg tactttcatt cggtataacg
    86401 ggaatattgt ttttctgagg tattacataa ctgtatgtct ctttgtttac ttcctttaat
    86461 ctttcataga ccctttttcc cgcaattttg aatgtaatct catcggaaat taccattatt
    86521 ctgccttttg ccttgttaag aataaacctg attgcttctt ctgtaccgtg ctcggatatc
    86581 tctatccttt ccattttcgg atcatgaatc aacccgcact cacatggata tatgttttta
    86641 ataagttctt ttatctccaa atctttgagt ttcatgtttt ccctctaata tataagcttc
    86701 aaaatttcat atagtccgaa acccatgaag tttcctatcg cataacccaa caagcctgtc
    86761 gcaattccta taagtataac tccctcattc tttatggctt tggccgtggg tataataaac
    86821 gcaggtccga agattcctgc tattgatgtg attattatgg tatccgcatc aatcttaaag
    86881 agcagcgcaa tgaaaaagtg taaaaacatt gttacaaaaa gaacaaacgc taccatgcct
    86941 aaaacataca cggactggcc gagtataagc ttcaggtccg ccatagagcc gaccgccaat
    87001 gaaaacacat atatgaagta ctgtcctatg gaataggata ctttgttttg ccttacccct
    87061 tttacaaagg ataatgccac tccgataacc gatactccga gcatgattat agctacgttg
    87121 gttgcttctt ttgtcagcag ataagaaatc cctatgctta cgccgagtgc tgctattccc
    87181 aacaggatgg caataactcc ctgttttatc ttttctttta caggtatttc caccttgtcg
    87241 acaatgccct cttttttata ttcggtcttt tccgttgcta cggatatctg cctgctcaat
    87301 ttattcaata agggtttagc caaagtacat ataaacagaa agtacacccc tcccagcacc
    87361 tggtccgcaa gattggcaac tattatggtg tttttatcgg catttactcc gtaccctatc
    87421 gcaaaaagat tgggtgtacc tccggtatag caccctacca gcatggaggc tattttttct
    87481 ttttcataaa cattggggaa aaccaaaaca ctaacggcgg ctacaaccat tacggaaaca
    87541 cttaagatta gaaaagacaa tccggctttc tttgcatttt ttcttaatgt cgtaaaatcc
    87601 gagttaaaaa gaataagggt gattgctata ggtataagta ccgatgaaac gatatccgac
    87661 aaaccggcat ccaaaggcag tccgctgttt gcccatatta ttcctatagc atagcagaaa
    87721 ataatcgggc ctactgtctt tataagcttt actttatttt ccagtttata cagtatataa
    87781 ggtattccga aaaataaaac aagttgaatt atttttgcta acataaatta ttccccctgc
    87841 gataattata tatcaaatat gtatctttgc ccacttcccg ctttttattc ttaacattcc
    87901 cgaaaaactc ctgacaaact gatcggcaag aagaccgagc catgctccga taagacctag
    87961 ttgcaacggg tataccatta tatatgagaa caacggacgt atcaccgcta cacttatcaa
    88021 cgccattgtc gccacaaatt ttacatcccc tgctcctctt aaacttcccg aatgcacaat
    88081 ctgtgtattt atacagaagg tagacagtgc cataatcaac aaaacatatc ttccgttttc
    88141 tataatgacc gaatcatcgg taaacagacc cataagcatc tttcctccga agataaatac
    88201 ggctgataaa acaacggaaa tgattattgc catccttcgc ccaattttaa tgtaaagcat
    88261 ggagaggtcg cttcgcttct cccctaaggt ttgccccact aaagctgttg ttcctgttcc
    88321 gagaccggct cctatatatg tagaaagatt cattatctgc atacaaacaa gatgtgttcc
    88381 gtaatctatg gtgcccagtt ccgcaacaat tttgttatac ataaaaaatc cgaaacgaaa
    88441 acataattct tccaatgccg cgtttcccga tatgttcgct atggatttta caatacttaa
    88501 atcaaacttt ttaaggcctt ttatatcaaa ataaataaaa gttgctcttc ttagcgaata
    88561 acatgctgcc aaaaatgcgg caaccattcc tataacagtt gcaatagcag ccccttttac
    88621 tcctaaagcc ggaaatccca atcttccttt tatcagcagg aaattaaaaa agacattgac
    88681 tacattggat ataaggcttg tggtcatagc tatcttggtg tttcccgcac ctctttgcgc
    88741 cgcatttatc gtcatggtca gaacaccgaa tactgtcccg gcagtaataa tacggtagta
    88801 cacaatagaa tcgtcaataa aatcttctcc ggctcctgca aatcgtaaaa gaggccttgc
    88861 ggtaactatt cctaacgctg tcataataat tgcaaggata aaacagataa gtagggcctg
    88921 catcagtacc cgattagctc ttttggcatc attctctcct tttcttctgg ctatcaatgc
    88981 tgtaacacct gtgtttaatg ccaaaaaaag cataagaatt atcattctcg gctgcgttgt
    89041 tattcctacc gccgtaaccg ccgttatgcc gacggaagat accattatca tatccacaaa
    89101 acttgtcaat ccgcctaaga gcgcttcaat tgtagacggc aaagccattt ttaacattct
    89161 gccgtatgct tcttttgccg aaggcagttc tcctaatttc ttgtgcttac gaagcatata
    89221 ttcaggctga tagaattgct taatcatact ctttattcta acatgtttat acctgtttag
    89281 tcatgtaaca tgtctttaac ataattttaa ctttaccatt ccttatttca gtgtttttct
    89341 cgcagtgtcg cctttttttc tttacttatt taagcaagtg aattattatt aaaggaggga
    89401 gtttttgaaa accgatttat tttccgaaag gaaggagata taaaaatgac aaaaaacaaa
    89461 atgttacttt catggataga agagatggct gctcttacta ctcctgatga gattgtctgg
    89521 attaccggag attctgaaca acttgaccaa ttaagacaac aggcaatagc aaccggagag
    89581 ctctataagc tcaatgaaga aaaacttccc gactgttatt tacaccgcac agccgtaaac
    89641 gatgtggcac gagtagaaca tcgcactttc atatgttcaa ggagagagat agatgccggg
    89701 ccgacaaata actggatggc gcccaaagaa gcctatgaaa agttgggcaa aattttcaac
    89761 aagagcatga agggaagaac aatgtacgtt attccctatt caatgggtcc tgtaggttct
    89821 aaattttcaa aaataggcat tgagcttacc gactcgatat atgtagtaat gaatatggtt
    89881 attatgacaa gagtgggcaa tgatgtactt gatgctctcg gaaattccaa tgatttcgta
    89941 agaggacttc acggtaaagc ggatatagat gaagaaaaca gatatatctg tcatttcccc
    90001 gaagacaata caatatggtc ggttaactcc ggatatggcg gaaatgttct tttaggtaaa
    90061 aaatgctttg ccctgagaat agcctcatat cagggaaaaa atgaaaactg gatggcggaa
    90121 catatgctta tactcggcgt tgaatatccc gacggcgaca ccaagtacat agctgcggca
    90181 ttcccttcag catgcggaaa gaccaatctc gctatgctta ttccgccgga aatttttgcc
    90241 aagaagggat ataaagtatg gacagtggga gatgatatcg catggataag aaaaggtccc
    90301 gacggaagac tttgggccat taatccggag aacggtttct tcggtgttgc tcccggaaca
    90361 aatataaaat ccaaccccaa tgcattacat gcaacggaaa aaggcactat tttcaccaat
    90421 gttgtccata atcttgaaaa caatacggtt tggtgggaag gattggacaa aaatcctccg
    90481 acaaaagcac tcaactggaa aggtgagcct tggacagccg aatcgggtga gaagggagca
    90541 catcccaaca gcagatttac cgctcccgca acaaattgcc cctctataag taaggaattt
    90601 gattctccga acggtgttcc tctctcagct atggtattcg gaggaagaag agcaaagact
    90661 gcacctttgg tatatcaatc tcttgattgg gtaaacggag tgtttgtagg atccataatg
    90721 gcttccgaaa caacggcagc tgcaagcgga gctgtcggag tagtaagaag agatcctatg
    90781 gccatgcttc ctttctgcgg atataacatg ggtgattact ttcagcactg gttgaatatt
    90841 ggaaaagatg taggagacaa ggttccaaaa atattccatg taaactggtt caggctggat
    90901 gatgaaggtc attttctatg gccgggattc ggagagaatt tacgtgttct gctttggata
    90961 ctggatcgct gtgaagataa ggtaggtgct gtagagactc caatcggtta tcttccgaaa
    91021 cctgaggaca tcaaccttga aggaattgat gttaccgagg aaacattaaa agaacttctc
    91081 tctgttgata aagaactctg gcttgaggat actgtcagaa tagaagattt ctataacaag
    91141 attggaaata ccgttccgaa agagctccgg aacgaattaa ctaaacttaa gaaaaaactc
    91201 tcataagttt atagagaatt tctgaaggtt gtactgacaa ggtgcaacct tctttatgtt
    91261 cggaaatata aataaatttt cacttatcaa cagattttct gtggataagt gtttttctgt
    91321 ggataacttt aaaattttgt taaatttttt aaaaatttct ttttttttcc tcttgtgcta
    91381 tgtttacttc tctgatatac tatgcttccc tgttgataac actgttttat ccacaggtca
    91441 cgaaggataa atctgcttgg ggcgaaaagt ttcagaaagc agttttatct tttttttaat
    91501 aattagggga aactaacatt tatggccgta caagaaatat ggaacgatgc tttgacgaat
    91561 attgagagtg taatgggtga acttaatttt aagacatgga ttctgccatt agagccttac
    91621 actatagaag gggacaattt tgtactgata gctcaatcag acttttcaaa gaattatctt
    91681 gccaattaca agcttctttt aaagaatgcc atttccgatg cggccgctaa agatctcagc
    91741 cctcttttta agttaaagaa cgaaatcggg gaatatgacg aaaacgggaa acatgaagat
    91801 ttccgttcca ataattatgc agattctcag cttctggata aatatgtttt ttccaatttc
    91861 gttacaggtt catcaaatca atttgccaga gctgctgcgg aagctgttgt ggacaatccc
    91921 ggtaccacat ataatccttt atttttatac ggagaaagcg gaataggaaa aacgcatctg
    91981 atgcatgcta taggaaatgc aatttctcaa aaatatccgg ataaaaaaat cttatatgtg
    92041 caaagtgaaa cctttacaaa tgagatgatt tttgctataa aatcagcaaa aagtaaagaa
    92101 ttcagagaaa aatacagaaa tttggacgtt ctgcttattg acgatattca gtttattacc
    92161 ggaaaagaag gaacgcagga agagtttttc tatacgtttg aaaaacttta ttattccaat
    92221 aaacagatag ttattacttc cgacgttcct ccggataaaa tgacaagact ggaagagagg
    92281 ctgaaaacga gattccagca aggacttatg gcagatatgc agacacccga ctacgagaca
    92341 agagtagcta tcctggagca gaattcctta aagtcggaat ataatatctc caaggaagta
    92401 atagagttta ttgcaaagaa tatatcgtca aatataagga accttcaggg agcgttaaat
    92461 aaaacggttg cccatgcacg cgttttcggt gaagacataa ccatcgatct tgccaaaaag
    92521 gccttaaaag atatgattga cgaaagtaat gcaaatgata taaccatacc gagcataatt
    92581 gataccgttg caaaatatta taatataaca acaaaagata taatttctcc gacaaggaaa
    92641 agtgctattg cttttccgag acagattgcc atgtatatat gtagaattaa aacacctttt
    92701 tctcttccgg taataggtaa atattttgcg gacagagatc ataccaccgt tttatatgct
    92761 tgtgacaaaa ttgcgaaaaa aataaaaacg gataaaaatg tagaaaaaga agttaatgag
    92821 ttgatagaaa agctgaatta ttaacaaatt tctgtgtaaa tgttaatatc tttctgtgga
    92881 atattgtata attcatatat aagcgtagtt tacagttttt attaacaaaa attatccaca
    92941 gtaaaaagaa cggtaaataa gggataaaat acgttataca caatatccac aagacctact
    93001 actactgctt atagatatgt tattaaaaga aagaaggtaa acaaatgaat ttcacgtgtg
    93061 caagaaaaaa attacaagat gccgttaata gtgtatcaaa agctatttca tctaatccta
    93121 taatgcctgt actgggaaat ataaatataa aagccaaaga ttctcagata atattcagag
    93181 gatatgatct tgaaatagga atagaacata ttgaagatgc ttttataaaa gaggaaggtg
    93241 aaatactggt taatgcaaga ctgttttccg acatagtaag aagtctcgga gaagaggaag
    93301 tggaaatatt caccgaagaa agtaaattgg ttattatttc gggaaatgcc gtatttaata
    93361 taagatggct ggatcctgct gcctttccta aattaccata tgtggaaaaa gataataaag
    93421 ttaccattaa gacggaatat ttaaaaaata tgataagaaa cactttattt tccgtcagta
    93481 caaatgaaca gagacttatc ttaaccggag cgttgtttga aagtgaagaa aacagtttta
    93541 agatggtaac gctggacagt ttcagaatga gtataagaaa agaatatata ttaggtaata
    93601 aagaagagtt taattttgta ataccgggaa aaacattaaa tgaattactt aaattaataa
    93661 caaatgatga tgaagatata agaatttatt atgaaaaaac acaggtactg tttgaattcg
    93721 gtaactgtaa atttatttca aaacttttac agggggatta tttcaattat aaggcaatta
    93781 ttcccactaa agactttgca acaaaaattg agataaatgt aaacgaattt aggaatatta
    93841 tcgaaagaaa tatgcttgta tcggatgacg gaagaaaagc tccccttata ctggaaatag
    93901 ttgaaaatat gctgacggca aatacgcagg gtgaagtagg taatgtaacg gaaaagtgtc
    93961 atataaagaa ggaaggaaag gatatcagga taggatttaa tgccagatat ttactggatg
    94021 ccattaaatc aatagacgaa gaaaaggtaa gaatatcttt tatgggagaa acaagtgcgt
    94081 gtataataac tcccctgaat agcgacgagt tcctttatct tattcttccc gtacgtatat
    94141 cggcgtatta atgttaatag aaagaattgt actgaaggat ttcagaaatt acgaatatca
    94201 ggaaatatcc cccaataaag ggattaatat aatatacgga agaaatgctc agggaaaaac
    94261 caatatcctt gagtcgattt ttttatgctc cacagcaaga tcacacagaa caaataaaga
    94321 tgcggacctg ataagaaatt tacagcaggg ctattccgta agaattgaag gaaaaagaga
    94381 cgaagaacct ttttctatag atataaattg tacaaaaata tcctccagac aaaataaaag
    94441 agttataaaa gtgaacgata taactctttt aaaaacagga cagctgatgg gagtattgaa
    94501 tagtgttatg ttttcaccgg aagatcttac cgtgataaag gaaggtccga gcgaaagaag
    94561 aaggtttctg gatattttaa taagtcagat taattcgtca tacttttact ctttacagga
    94621 atataacagg ataataaagc aaaaaaatgc acttcttaaa agagtgtttg aaacaggaaa
    94681 gaacagcgat cttattgatg tatataatat aaaacaggca aaaaccggaa cgaaaattat
    94741 aaaagaaaga gcagctttta cggaacaaat aaatgaaaaa gcgggacaga atcattttga
    94801 tatagcaaac ggcacggaag agctggatat aagatacgct tgtgatattc cggattattc
    94861 aggcgaagaa agcgactttt ataaagcatt ggataaaata aaagataaag aaataaagat
    94921 tcaaagtaca atattagggc ctcatagaga tgatgttgat atattattaa acggattaaa
    94981 tataagagat tacggatccc aggggcagca gcggtctgcg atattgtcat tgaaatttgc
    95041 ggaaatgcag attattaaag ataagacaaa caaacagccg gtgctattgt tggatgatgc
    95101 attaagtgaa ctggacttat taagaaaaag aaaattgttg aacaaaatag aaacaaaaca
    95161 gacttttatt acctgcaccg aaaaaagacg atatatgacc ctggatgaca aaataagttt
    95221 tttctatgta agcaagggat cagtcaaaag tatatagaaa aaagaccgta aaaatgctat
    95281 aattaacaga agaatgaaga gaggatttca tgtttttaca cataggagat aatttggttg
    95341 tgcgcgaaaa agaggtaata ggaatatttg attttgaaac ttctacacta tccaagcaca
    95401 caaaaaatta tctcagagat aaagaaaaaa aaggaacaat aacaacagta agtgacaaac
    95461 ttccgaaggc ttttgttgtt gttaagaaaa ataaggagtc cgatttccgg atatatctca
    95521 cactgatatc ttctgcaacg ctaagaaaac gagctgaaaa caataaaata acggagtaat
    95581 atatggataa taaaaaaaga caggataata taagagattg caattacgac gagtcgcaga
    95641 tacaggtact ggaaggtctt gaagcggtca gaaaaagacc cggtatgtat ataggaacta
    95701 catccgtgag aggattacac caccttgtct atgaaatagt ggcaaacagt attgatgaag
    95761 cacttgccgg aagatgtgac agaattgaaa taacgataga taaaggcaat atagttacgg
    95821 ttacggacaa cggcagcgga atcccggtag gcatacatcc taaaatggga attcccacgg
    95881 ttgaagttgt gcatacggtg ttacatgcag gaggaaaatt tgattcttcg gcatacagta
    95941 tatccggagg actgcatgga gtaggagctt cggtagttaa cgcactaagc gaatttcttg
    96001 aggtggaagt caagagagac ggaaaaatat ataaacaaag atacgaaaga ggcaaaacgg
    96061 tttcaaaact ggaaattatt ggaaatacta acgaaaccgg aacaaaaacc gtatttaaag
    96121 ccgatgaaca gatttttgat actattgaat atgaatatga tgctctctta tcaaggtata
    96181 gagaaatggc atttctgaac aagggtgtca tgatagtgtt aacagataaa agaggagagg
    96241 aagaagtaac aaacgaatta tgttatgaag gcgggatagt atcatttgtc agatatctgg
    96301 attcaaacaa aacaagaata cataaagata caatatatat tgaagcggag aaggaaaatt
    96361 gtcttgtgga gattgcgatg cagtacaatg atgactacaa tgagacactt tattcatatg
    96421 caaacaacat agcgaccagc gaaggcggaa tgcatgttac aggttttcga tcagctctga
    96481 caaaagtaat aaacgattac gcaagaaaga taggagcact taaggaatcg gataaaaacc
    96541 tgaccggaga ggacgtaaga gaaggcttaa ccgtagtatt atcggtaaaa ttacacgatc
    96601 cgcagtttga gggtcagaca aaatcaaaat taggtaacag ttatgtgcga accgccaccg
    96661 agcaaacggt aaatgaggag ttggcagcat attttgaaga gaatccgcaa gaaggaaagg
    96721 taattattga aaaatgtatt cgtgcatccc aggccagaga agcggcaaga ggagcacgcg
    96781 aattggcaag aagaaagact gcactggaag gaaatgctct tcccgggaaa ctggcagact
    96841 gttcggaaac cgatcctaca aagagcgaaa tattcatagt ggaaggagac agtgccggcg
    96901 gaagcgctaa acagggacga gacagaaaat atcaggcaat acttccttta tggggaaaaa
    96961 tgcttaatgt ggaaaaagca agaatagaga aagtatataa taacgacaag ctctctcctt
    97021 taataactgc attaggcacg gggatcggcg aagattttaa tattgaaaaa ctgagatatc
    97081 ataaagttat aataatggcc gatgcggatg ttgacggagc tcatatagga atgctcctga
    97141 ttacttttgt atacagatac atgcgtgaac ttatagaaaa aggacacctt tacctggctc
    97201 aaccgccttt atacagaata accaagggaa aagattcaat atatgcgtat aatgaagcgg
    97261 aaaaggacag aatattacga gaaagagact gggacagtaa agattgtacc cttcagagat
    97321 ataaaggact gggagaaatg agttctacac agttgtggga aacaacaatg aatcctgcca
    97381 ccaggacaat tatacagata aagatggata atccgttcct ggcagatata gcggtgtccg
    97441 atttaatggg ggatgatgta caacccagga aagaattcat tgaaagacac gcaaaagatg
    97501 tacaactgaa cagattagat atataatgtt tcacgtgaaa catatgaagg ggtcgatatg
    97561 ggaaaaataa tagcagttgc caaccaaaaa ggcggagtag gcaagacaac tacttcgata
    97621 aacctaagtg caagccttgc atttatggga aaaaggacac ttcttattga taatgatccg
    97681 cagggtaatg caacaagcgg tctgggagta gacaaatgga ccgttaagca gtcggtctat
    97741 gatgctataa ttaatgatat agacataaaa gagacgattc ttcctacaat gttggatagt
    97801 ttattccttt gtcccgccaa tatacagtta gcgggggcgg aaatagagct tgtaagtttg
    97861 atgatgcgtg aaaacagact gaaaaatgct attaaaccta taagaaatga ttatgattac
    97921 ataataattg actgtcctcc atctctgggt ctgctcacat taaactcact aactgcagca
    97981 gacaccattt tagtgcctat acaatgcgaa tattatgcgc ttgagggctt gaaacagttg
    98041 atgaagacgg tttctttggt acaaaagcat ttaaatccct ccttaaaggt tgaaggagtg
    98101 gttatgacta tgtttgattc aagaacaaat atctcacttc aggtagtaga aagcgtaaga
    98161 cagcactttt ccgacagagt atatagaaca ataataccga gaaatgtgcg gttaagtgaa
    98221 gctccgagcc acggactacc tatcattcta tacgataaag actccaaagg agctatgagt
    98281 tatttggatt tagcagaaga agttatacaa tgcggagggg aaaatggcta aatacggttt
    98341 aggaaaggga ttaggtgcac tgctgggtgg tgattatacc gatatttccg ttattaacaa
    98401 agagaatcag ttgattgaaa tggatattga taagatacag cgggaccccc ggcagcctcg
    98461 tagagacttt tcgaaagagg aaatggatga acttgcggaa tctgtacgcg aaaaaggcat
    98521 aatccaaccg atattggtta ggaaaacaga tgatacatac acaatagtgg caggagaaag
    98581 aagattccgg gccgctaaac aggaaaagct gaaaacagtt ccggtaattg taaaggaatt
    98641 taccgataaa gaagttttag agattacttt aatagaaaac atacaaagaa aagatttaaa
    98701 tccgattgag gaagcggaag catataagag attatgtgat gaattcggag caactcagga
    98761 ggaaatagca aaaactctgg gaaaaagccg tccggtcgtc acaaacaagt taagactgtt
    98821 gaatttagag gaaaaaatac tcagttttat aagaagagga aaactttccg aaggtcacgc
    98881 aaggctgttg ttgtctgtcc atgaagacat aagagaggat cttgccgagg aaacggtgaa
    98941 caaagaatta agtgtaagag accttgaaaa actcataaaa aagacaaaaa ggactcccgg
    99001 aaagaaagaa caaatagaaa acgatgaaga atttgcaaaa gttgagaata aactacagaa
    99061 gaaacttgca acaaaggtaa caataaccag aagtattaag aaaggtaaaa taacgattga
    99121 atattattcg cttgaaggat taaacagaat catagacata ttaagcagat aatgtttcac
    99181 gtgaaacaaa gtaatggaat atctaatata cgcatcaata agtttaggaa tatcactgat
    99241 tgtgtcatca attacagtgc tgatttttgt taataagaaa aaaaagaata aaaagcggga
    99301 acaagagcgg gaaatctcta tagataatat aaagacatat gaagatgtac tgcgtcagta
    99361 gaacaaatta catgaaaata ctaaggaaaa tgagcgtatg atcagatatt tggacatgca
    99421 gagcgaaaaa ttgaataaac tgacaacaat atcatttaaa aacgtaggcc ttgtaagata
    99481 taaatattcc gatgatgcag gaggcaatct cagtttttca cttgctttga tggatgataa
    99541 acagaacgga atagtgttta ccaatatctc gttaatagac agcaattcct tatatgtaag
    99601 aaggataaca aacggagtat cggatgtcct tcttatggaa gaagaaaaag aggctataaa
    99661 acaagcaaaa gaaagttctg taatttaaca atatttgtca tatacataac tattgacaca
    99721 tttatttaac tttggtatat tcattaggcg cgtttgcgta tgtactttga acagtagaca
    99781 cggagagatg gtcgagttgg tttaaggcac cggtcttgaa aaccggcgta ggggcaaccc
    99841 tacctagggt tcgaatccct atctctctgc cacacggaga agtacccaag tcggctcaag
    99901 ggggcggttt gctaaaccgt tagtaggggt aactctagca tgggttcgaa tcccatcttc
    99961 tccgccataa tttgtccgca aatcggttcg ggtttgcgga cattttgatt tcttgcgtaa
   100021 aagagtaaaa ttctttaata taattttttt aaaaagttac aaagaaataa tgttatatac
   100081 aactaacact tagcatattg tttaatttaa tatcttcatg tcggatattt tgcattaaag
   100141 tccggagatt tttaacggtc attgtgtttt agctacactg agtatactat atgtagtaga
   100201 gataaaaggg aatataatta tgcaaaggtc tgaaattaaa agaccttgac attttacatc
   100261 tcaaatatat ataatctaat acgctgtgca tgaaagtgta caaaaaaatg agataaattt
   100321 tttacatata ggagttactg atgaaaagaa catatcagcc gaagaccaaa caaagaaaca
   100381 gagtccacgg attcagacaa aggatgaaga cggctgccgg aagaaagatt attaaaaaca
   100441 gaagagccgg gggcagaaag agattatctg cataggattc ctttctcatg cctttattta
   100501 tacgaaaggt taaatatgga aacgctgaaa aagaactatg agtttttacg tgtatacaga
   100561 aaaggaaaat atttcagcgg aaagtatttg acaataaacg ttctggaaaa tgcatttttg
   100621 cgcaataggg taggcatatc cgtaagtcgc aaggcataca aaagcagcgt taaaagaaat
   100681 ttataccgta gaagaataaa ggaaatatta aggacaggta actttaatct gaaaaacggg
   100741 tatgatatgg tattcgtagt taaaaaaact gatgaaatac ctgaatacca tgaaatagag
   100801 aaggaaatag tcgatttgct ggataaggca aaattgataa taaagaatga aaaaactgat
   100861 aatcagattg attaaatttt acagaatcaa tatctctcca aataagttgc cgtgttgcag
   100921 atttcttccc acctgctctc agtatgctat tgaagctgtg gaaaaatatg gcgctttcaa
   100981 aggcagtttg ttgtcattgt ggagaatttt gagatgtaat ccgctttgta aaggcggtta
   101041 tgatcctgtt aaataaggaa aggaagggct atgtttgatt ttatttataa ctttttcgga
   101101 aagatatttg aattgcttta taccggtctt tgcggacaga atttcggaat aaccataatc
   101161 gtatatactc tgctcttaaa gcttctcttg atgccgcttt ccattaaagg gcaaaaatcc
   101221 acaatgagaa tgacagaact cactccgaaa gtcgatgcaa ttaagagaag gtacaagaac
   101281 gatcagcaga aacaaaatga agaaattcag aaattgtata aacaggaaaa tgtaggttgc
   101341 gcatcaggct gtctgcccac cctgattcag tttccgttgc tgatagcaat gtaccgagtt
   101401 cttgcacaac ctcttactta cattaaagga gtttccaaag acgtaatcaa ccagattata
   101461 gagaggctgg gatatgctcc tacggtttta cagaccacta taaacaacga ccttctctcc
   101521 gaccctgcta aattcgagct tgtcaaggat ctcatatctt tcgacaaact tattgatatg
   101581 aattttttag gaatgaatct cggcttggtt ccgaaggtta atccttccat acttttcggt
   101641 gcggaaacta tagggacata tcttcccatc ctgttcctgc cgttaatttc agtgggattg
   101701 gttatacttt cacaatatct taatgataga gctatgaaga aaaggatgcc tgctaagaaa
   101761 aaggctccca atccgaacga cgctccggat atgtccgaac agatgggcaa gacaatgaag
   101821 ttcttaatgc ccgctatgac attatggata ggatttacat tacctgtcag tatgtcgcta
   101881 tactggatat ttacacatat tgtccagata attcagcatt ttataatgga gaaaataagg
   101941 aaaaaccacg atgcaaaaaa agaagccgaa gcggtaagaa gcggtgccgc atcgcagagt
   102001 gaagaaatca ttgaagcaac atatgaagtt aaagaggaaa gcaaggagag taaagataaa
   102061 aaatgagcag ggttagcatt gaaaaaacgg gtaaaacagt tgatgaagcg gtacaggcag
   102121 cactcttgga attaggcttg gagcttggag atgtggaaat agagatagtt gaggaggaga
   102181 ccaaggggct tttagggctg ggaaggaaag aagcaaaagt aagagtatat ttctctacgg
   102241 aagacgatcc caaagaaaca ataaagtttt tcttggagga tatactttca cagatgaatc
   102301 ttgttcccga tgtatttgtc agcgaagaag acggttttta caaggcggaa ataagctgta
   102361 acgattccgg acttattatc ggaagaaaag gagaagtgct ccaggcatta cagtttataa
   102421 catcacaggt agtaagcaga gcttatgaca catatataag ggttataatc gatacggaga
   102481 attacaggga gagacacaaa gcatacttag aagagttagc aagaaaaact gccgataagg
   102541 ttgtcaaagc aaaaagggat tttatgctgg atcccatgtc ttcatatgac agaaggataa
   102601 tacacacatg tcttcaagac agggagaacg tttctacata cagcatcgga gacgagccat
   102661 atagaaaaat agttatttcc tttgaaagag acggaaaaag aaacagctat taataaaaat
   102721 ggaacatact atagcagcaa tttccacacc tgtaggggtt ggaggaatat cggtgataag
   102781 gattagcgga agcaaggctt tttcggttgc tgaaagtatg ttcaagagta aaactccttt
   102841 tagagatatc aaaagtcata cagtaactta cggacatatt caagacaaaa aaggcaatac
   102901 attggataat gtactgcttc ttaagatgaa agctcctaaa acttatacca aggaagatac
   102961 ggtggaaatt caatgccacg gcgggttata tgtaacaaga aaaatacttg ctttggcata
   103021 taccaacgga gcggttcccg cacaaccggg agagtttacc aaaagagcat ttataaacgg
   103081 cagcattgac cttatggaag cggaagcggt catggacctt ataaacagcc aagcggaaga
   103141 tgcttacagg gaagctttgt cgcaaatgaa aggggagaag agtttacaga ttaagagtat
   103201 aagaagtgaa cttatcgacc tcatatcaca cataatggtg attatggatt atcccgaata
   103261 tgatacggaa aagataacgg cgaaagaagc acaggataaa gcacaagaca tagcggacag
   103321 actcaaggta ttcgttgagg attttgatga aagaagaatt atacgggaag gattgactgt
   103381 agcggttata ggggatgtaa atgtaggaaa gtccacattc ataaacagag catgcggaga
   103441 gaatcgtgta attgttaccg atatagccgg gacaacaagg gacgtggtgg aagtaaaaat
   103501 tgatgtaaaa ggactatgtg ttacattaaa agacactgcc ggcattcgca gtacggataa
   103561 tgaaatagaa aaaataggca tagaaaaatc caagcaggcg atagagcaat cggatgcggt
   103621 tatatacatt gcggatataa accagccgta taaagacgag atactccatt acctgagaga
   103681 cagggataaa gtgatatgcg tactgaacaa gaccgatctt ttattggaaa aagacaagga
   103741 taaacatatt gaatctatgg agaaagagct taaaaaatta ttcaaagaca gaataattgc
   103801 ctgctcttta gataaaaaag aaggaatcag cgagattttt gacaaattgc tggatatggt
   103861 taacgcagga agtataagga gcaaagactc cgcaataaca aacgaaagac ataaaaaaca
   103921 tatactggat gccattacaa atcttgaaaa cacaaaagag ggttctttta tagatatgct
   103981 cagtattgat ttaacaacgg ctgcggaaga actgggtaaa ataacgggag aaaccgcaag
   104041 cgcggatatt atagacagaa tttttgagaa attctgtata ggtaaataaa atgaaacaat
   104101 atgatgtagt tgttgtcgga gcgggacatg cgggatgtga agcagcactt gcctgcgcaa
   104161 gaatgggatt aaaaactgca attttcactt tgaatctgga cagtatcgcc aacatgccgt
   104221 gcaatccgag tataggaggc accgcaaagg ggcatctggt gagggaaata gatgccttgg
   104281 gcggacagat tggtaaatgt gccgatgcaa ccctcataca gtcaaaaatg cttaacagat
   104341 ccggaggacc tgcagtttat tctcttcgtg cccagataga cagaaggcag tatcagaccg
   104401 tgatgaaaca tatgctggaa aagcaggaaa atttggacat ccggcaatat gagattaccg
   104461 atattatttt tgacaacaat agcggagtta ccggagtggt taccaatacc gatgttgtca
   104521 tctccgctaa agctgtaata atagcgaccg gaacgtatat gaagagtcgt attataatcg
   104581 gagacatgtc atatgaaggc ggacctgacg gttttttgcc tgcaaacaga ctgtccctca
   104641 atctggaaaa aaagggaatt aagttaagaa gattcaaaac ggggacaccg gcaagagtac
   104701 ataaaaacag tattgattta accaaaatgt ccgttcagaa cggtgatgag cctattgtgc
   104761 ctttctcttt tgaaacggat aagattgaaa taaaccagat accttgttac cttacatata
   104821 ccaatgaaaa aacgcatgat atcatcagaa aaaatctgga cagatctcct ttgtacagcg
   104881 gcaagataag cggcacggga gtacggtatt gtccgtctat tgaggataag atagttaaat
   104941 ttgccgataa ggagcggcat caggtttttc ttgagcctat gggtttgatg accgacgaga
   105001 tgtacgttca gggaatgagc agttccatgc cggaggatgt acaggaagca atgtataaat
   105061 ccgtaaaagg catggaaaat gtggttttca tgcgcagtgc atatgcaatt gaatatgact
   105121 gctttgatcc gttgcaactt atgcccacat tggagtttaa aaaatttccg ggcctttatt
   105181 ccgccgggca gagcaacggc agcagcggtt atgaagaagc tgcggcacag ggaataatgg
   105241 ccggcataaa tgcggcattg aagataaaaa aacaatcatc ctttgttctg gacagatcgg
   105301 aggcatatat cggagtactc atagatgatc tggttaccaa aggaacaaac gagccgtata
   105361 gaatgatgac cagcagagcg gaatatcgcc ttatattaag acaggacaat gcggatgaaa
   105421 gactgacacc gaaaggttac aagttggggc ttataagcga agaaagatat accgggtttt
   105481 taagaaagat ggaaaacata gaaaaggaaa tagagcgatt aaaaaagaaa aatgtttctc
   105541 ctaaaattgt taatccgttt cttgaaaaaa gaaatcagca acctttaaat tccggaacaa
   105601 ccgaagcggc tattctgaag agagagggtg tcggctataa tgacctgtat gaaattgacg
   105661 atgagattcc cgaattgact aaagaagaaa gagagcaggt cgagataaag ataaaatatg
   105721 aaggctatat agaaaaacaa caaaggcaga tagagcagtt taagaaaatg gaaaaggtgc
   105781 tgctttccga aaatatagat tacaaaaaaa ttaaaggtct cagaaacgaa gcgcaacaga
   105841 aactggacaa cgtgaaacct gtttcactgg gtatggccgg aagaatatcc ggcgtatcgc
   105901 cggcagatat acaggttctt tatgtttata tcgaacaaca aaagcgaaag aaaaggaaag
   105961 cgaaagaatg aagcaacttc ttaaaggtat ttttaaagag ctgggaatag aataccaaga
   106021 ggagcattac gataaattcg acacatatgc atctcttctt atcgaatgga ataaaaagtt
   106081 caatcttacg ggaataaccg atttgagaga tatttacata aaacatttcg gagacagtct
   106141 cttgttgtat gagggaataa aagcaaccga atcgttaatt gatgtgggaa gcggagcagg
   106201 ttttcccggt atacctttaa agatagcggg gtacaaacaa aaaacagtat tacttgaagc
   106261 aaacggtaaa aaggcttctt ttctggagct ggataatatt ttcgtatgca ataagcgagc
   106321 ggaaaacgcg gcaagagaag aagcgtatag agagcttttt gatgttgttg tagcaagggc
   106381 tgttgcgccg ttgagtatac tgttggaata ttgtgtaccc ttcataaaaa gagaaggcat
   106441 ttttatagcc atgaaaaccg ataactccga actgcaatcg gccgagaatg ccatgaagca
   106501 actttctgtt gaaatattaa aagaagacaa gcagaccatt cctttttccg atattaaaag
   106561 aaataatatg tattttaaaa aaacaagaaa tacagcatat aaatatccca gaccggacgg
   106621 ggttcccaaa aagaaaccgc tataaaattt cacagagatt taacaggtta gtcataattt
   106681 attcacatgt tttctttatt atgagagcac aacaatttca taatatattc ctcataaaag
   106741 cagaaagact taagtccttc tgtttttttt cttaaacgct tttcttgcat gctgcctgca
   106801 ttttatgagg aacaaaggaa tgttaagcat tcttttaaat cttgcaggct gttttagcag
   106861 tctgtagaac cattccagct tatgcttgca gaagaactcg ggagctcttt tggaaaagcc
   106921 cgaaataata tcaatggttc cgcccgctcc gatagcaagt cccgaatgta tgttatggcg
   106981 gtgtttgtat ataaaaaact cctgcttggg agcgcctaat cccacaataa tcaaatcagg
   107041 tttttcggta ttaatattat caattatctc tttttcctga tcgacgttaa agaatccgtc
   107101 atgataaccg gctactgtta tatgcgggta aagggattga attttttttg ctgcggtact
   107161 tacggaatca tgtgatgcac cgagcaggta aacggaaaaa cttttaccca tgcctaaaag
   107221 attctgtaca aatacacatc cggggacctt ctcttttaag ggagtcttaa gaatttttga
   107281 agcaagaaca actccgtctc cgtcagggat attcataaag gaggaattta atacctccat
   107341 aagactatta tctttaattg cagccataca tatttccgca ttgggagtaa agatgtatct
   107401 gaaagaagca cggtcggata aagcatcaca tgctttttcc aatgcctttt ccatatctgt
   107461 gttgtcgatt tttataccca agatatccat aattgctcca attactttga agtataggaa
   107521 aaaacaagtg aaaaatcaat aaaaaggttg attattgatt aatatggata tagaatgaac
   107581 ttcgcagaca tgagaaaaac caatgcttta aagttaatca tcattatatt gataatgtcg
   107641 gcagtacttg tttcgtgtac cgaagaagag taccgcgctt ccaggctgta caaatctttg
   107701 acatcatatg agcaaaagag tgaaacggtt actctgaaaa acaagaaata caacaaaatt
   107761 gatttatcca aaaaagccgt attggaactt tccaagggca tgatatataa cgtggatttc
   107821 agtgaagcgg tcaatgtcag agatgcttca accgcctcca tactgaattc ggaaataata
   107881 tccaaaacac ttgccatgaa agtgacagaa aaagatacag ttatgaatgt gatatctacc
   107941 gatatcgcat catatgggga ttatgttata agcgtaacag ataatgctgt atttacaggt
   108001 tcaggactta aagtaaacaa tttaggagaa ggcggagcgg caataaagat ttcttcgggc
   108061 gcatccatgg tattaaataa atccaatgta aaggcaaatg gcgccggcgt ggaatccgat
   108121 tcgctggtaa gtataagttc cagcgaaatg acggtggaaa gcctgaggtt ttacgaagga
   108181 gcgacagtta ccctggatga ttcaagattt tacacaaatc acggtataat gcttctggac
   108241 aatgctaatg aaaaattgat tcatgtttca cttaatctta aaaaagctaa actgaccgcc
   108301 gacgacggag cactgctgtc catgatagat acaaaggcct cagttaagat tgaagatacg
   108361 acattaaacc agggttcatc gaatattatg ttgttaaaaa attccgaagc aacagttacc
   108421 ttgtgcaact ccgatgccga aggcggagta atgaccgatg aaagttcttc tcttaacttg
   108481 cttattaaga acggatcggc cttcaaagga tatataaaca aggggaacag aacaaaaacc
   108541 gttaccgtcc agatagagga aaacagtgtt tgggaagtta catcggacag ttatgtcaga
   108601 ggtgttatat taaaggatgc aaatttcgaa aacataaaat ccaacggatt tacaatatat
   108661 tatgacccca aaagttcaac caatgcctgg ctggataaaa agactgttaa tcttccggac
   108721 ggaggaagac ttgcgccgtt aagataataa gaaactaaag gtgctaacca ataatttttg
   108781 atgcattagc catgaattta tcatacgtgg agcccagaaa gaaacggctc ctttttaaag
   108841 ttctgtaaga ataaagatag ttgtatatat gtgtatcgct ggcaataaaa ttaatcaagc
   108901 ctttcttgac atatctgcgt attcttaaag cctgaagagg agaataaagg gaatatgcat
   108961 ttatctgagc aagtacatcc agtccctcaa gcttctgcac ctgtatggga ctatatcttt
   109021 ctatatgagc tattattacg ataagcccca tagactgcag atttacaatt tcgttcagaa
   109081 gcatattgtc ccaagaagaa aagggcattt ctataagtat ataatttgtg tcttctattg
   109141 taagtttgcg gattttctta ttattaccga gtcccgtcgt cagccgtact tccgctccct
   109201 tatacagttt tatatcggtg ttgttagcgg ctgataattc taaaaaagcc ttatctcttc
   109261 gggaaaggaa tctgtcaatg ctttctttaa aaggataaaa atgaggagta cagacaatgg
   109321 aagacacacc gaacttgtca gcggttttta attgtcttaa agactcttcc aaagaggaag
   109381 agccatggtc gacttttggt aatatatgag aatgtatatc aatcattgtc aagattcacc
   109441 ggcggtgtca caggtttatt gtcgtatccg ccgtatcttt catacttata cccgttttta
   109501 ccgtatctgt attgtttgtt ataggacatt gcttcaatgt tataatcgtt gaatataaaa
   109561 cctaaaaact ttccgtttac tttgtttata gcatctaccg catccttaat aagattagtc
   109621 cgcgagtaat tctgtctgac agccagaacc gttcccgtaa ccttatccga tatagccagc
   109681 gcatccgtga caataagtat agggggcgta tcaataaata tatagtctaa agtattacgc
   109741 aattcactca gcaataaagt cattctctcg gaagtaagca gttccgacgg attgggggga
   109801 atggtgccgc tcggtaaaac atgaagattt ttatatttcg tgggatatat aaagttctcc
   109861 ttcacaagtc cgccgagaat atcactgagt cccggtgagg attttatctt aaagtactta
   109921 ttaatagtgg gcttacgcat atccgcatca ataattaatg tcttaaatcc cgcttctgca
   109981 aaagctaaag ccatattggc acagacagta gattttcctt cacccatatg tgagcttgta
   110041 aacgagataa ccgggcattc atctttcgca gtaatgaaca tcaagttggt tcttgcagct
   110101 ttaattgcct ctgcaacggg aaaagatttg ttatcaaaaa gaagaaactc acgtttttca
   110161 atgtcagcca taaaagtgtc ctttcttgtc ttcatcctga tatagtttca gatccggaat
   110221 gtacccgata acaggtgcct tgacaaccct cagcaggttt tcttcagtcc gcactttggt
   110281 gttaaataat tcatacaata taacaaaagc gacagcgaca ataaatccta aaacaactcc
   110341 gacagcaaca tttttaacca tattgggaga ggaaggtttt gacggtacga cagctctgtc
   110401 cagtatttcc acagctcccg ctttaatgac tctgagtatt tcctccggtg ctacatcaat
   110461 aaaggcattg gctataatca tagcatctac cggatcttcg cttttgatgg ttattttcat
   110521 aacttcggtg ttattcaccg aagaccctga aaacatcttt cttatctgtc ccggagtata
   110581 atccaggcct gtagtttcta ttaccttatt tatgacatta tcgcactgca ttataaccga
   110641 ataagtggct ataagtttct gcgaagtagt tatatccgat gaagaaatgg ttgtatccga
   110701 cctgttggag ttattaaata cgtacaatgt tgccgatgct tcatactgag gtgtaataaa
   110761 aaagtacgtt acagtcaaag aaagactggc cgcaaccaga acaatactga ctatcagcca
   110821 gaaatgtctt ttcagataag aaaatatctg tcgtatatca agttcttcca taaaaacatc
   110881 tcctacagtg gttattaata catcgtttat aataataagg tatcataatt ggcttgtcaa
   110941 gcaagacaca tacggataac ggcacggtaa gtgacgatta atctttctga tggagacagg
   111001 ctgagccgaa aaaacttttc cgtaaaacaa ccttattcaa caataataac accgtaaacg
   111061 gtgatattgt tgttcttgca tacttccaga attctgttta ctttttttac ctgcgaagag
   111121 cggtatttct ctacaagaat aattccgcta cagtcaagaa aagattttgc aatatcaaca
   111181 gaatcaaaaa agttgtttag cagtctccag tcataatccg tattgtcctt gagatatttt
   111241 gcaataatat ctacagcttc tatattttcc gaaccggtta ttccgatcgg atccgttgtt
   111301 tttccgcaag catgtattac aggtaccaat gcctgttcgg caattatatt tatatctttt
   111361 ccgacggaaa aaaggttcag accgggtatc tgctctatat ctataagttt actgcttaat
   111421 tgcaatctgg cgacaatgag gacatatgcc aaaaacattc ccaaaacaat cccgagtaaa
   111481 gtatacagaa agacatcctt gttatatgaa tcataattga attcatctat tacggtctgc
   111541 ttctctgcaa tttgtttatc tgcctcactg atagccttct gtatgtcggt atatgatgta
   111601 ttgtatgttt tctgaatgtc ggctaaagcg gtatttccgg acaagttttc cgtttcatac
   111661 agtaaagtga tgttaccggt acccaaagtg gaattaacgg tactgattgc tctttcgaag
   111721 catttatttg caataccctt taacattact aagtcatcag caccttttgc atacacatgc
   111781 atggtcaatg tattgtatga gacagttgcg gtaaaatcta caaaaatatc cactacttta
   111841 aaaggaacgg atataccgct ttctttgtat acttcagtaa gaagactctc atcactcact
   111901 acggcgataa gtagagcaac attcttctct atgttatata cagcagtata ttttttcagt
   111961 gaagaggaat taataggttt aatatacata tacagttcat ttttttcgta atcagccttg
   112021 ttttttaaca gtttatcctt gcgggcgact aaagaatcca gttctgtctg taaaacctgt
   112081 atgttctctt cttctttacg gtaatttgaa taatttgtga taatcttaaa aacacttgcc
   112141 aaaattgcac agcacaaaac aacaataact attaacacta tattcttacg caaatatttt
   112201 ataagtgtgg ataaatccat gtttcctccg aaagactttt acaatattaa aattatatca
   112261 cataaacagg gcaagaccgg taaaatcggt gtatttattt acatgttata ttgtcaattc
   112321 atagcgattt aatagtgaat gttaataata tacggctttt taaaattccg cagttcaaca
   112381 aagagtacat cggacagaaa atgaagtatt aaagaacggc tcggtcaatg gcttcttagc
   112441 attgtaaaat atggcgcttt actatataat tcaaatgtca acaagttggg aaagcactta
   112501 tgggtaaaaa aaagaccgtg aaaaaaactg aaaaaaagaa cacaaagaaa gatcttataa
   112561 aggatttttt ctatcttgaa caggatacaa aaaaattaac atattttgag attgtcacaa
   112621 cggcttttct ttatgttttt ttatcaagct ttgcacttat agtcagcgat aagatttatg
   112681 agaatataac ggcaacaaaa ctgtatgctt tgatatcaat gttcggaata tggtttatct
   112741 taatgtgcgt cggcttgttg gagaaaatac gttcaaaaaa ggtaaatgcg gaatacttca
   112801 aacaggctgt caaaaatctt tccgtaaccg atatagccct tatcgcgtac acgctggttg
   112861 cttttatttc tgcaatactt tccgaataca gagaggttgc atttatagga gataaggcaa
   112921 gaaatgaagg atttcttatt caattgatgt atatttccat gttctttatg ctctctcgga
   112981 catatgtgca taaactggaa ttgaagatat gggcgttggc cagcatgccg gtggcattaa
   113041 tagggattct tcaactaaac ggatattatg cagggaaatt tacttatgat atattgaggc
   113101 acggaaggtc cttcttaact actttaggaa acattgacgt ggtttcaaca tatcttgtca
   113161 ctcccataat gatatgtacg gttctgtact gtcaaacaaa agagaaattc ttcaggcttt
   113221 tatatatcgc ttcggcagga ctctgtttct atctttccat actgataggt gtagacagcg
   113281 gctttgtcgg aatggcaacc gcaatatttt tcatgttccc gtttattgca aaagacagac
   113341 tgtcggcatc caggatattc catattgttt ctctctttct tttccttata taccttgagg
   113401 atcagctttc caagataggt acgagacact tcgtattttt cgaaaaaccg ttcgatgata
   113461 agcttttctt aggagcgata gcggcaatta ttgtttccgt tataatatat tttgccggga
   113521 aagttataaa aacggaaaag aaatttgtat ggcccattgc gtactttgtt gttgccgctg
   113581 cgggtgtata ttttgccgta aagaaaatac ttgtcatgcc ggaacctacc ggttcatcgg
   113641 ttaagttggt attgtaccag ttcggacaga tattacaggg aaatatacag gaccattacg
   113701 gttccaacag aatatatgca tggagagtta tataccgatt ctttaaggaa aaatccgtta
   113761 tagggcacgg ccccgatacg gtacgcgctc tatggatgag agaagagtac atgtacagca
   113821 cgaaaaaata cggagtagtt tttgataaag cgcacagcga ctatctgcag gtactggtga
   113881 caacgggaat actgggactg acatcctatc ttgtttttca gttttctctg ctgataagat
   113941 cctttaagaa atttaatcag ccgttggtaa tagctttagt gacggcttgt atatgctttt
   114001 gtgcacaggc attttttaat ataggagttc cgattgtgtc accgtacgga tggatactgt
   114061 tcggtatgct cgcatcagta ctgaggaaaa aggaagaaac cgcctgattg aaggtgtttt
   114121 tggaggaata agaccttgaa aaaagacaaa aaaaacatca gcctatggat aaagaagata
   114181 atactggtag ccctggatat aatatcaata aacgctgcta ccattctgtc cctgtttgtc
   114241 cggtttgaac tgagtttttc aaaaatcgat gtacaatata ttgaaaacgc aaggcagttc
   114301 ctgtggattg atacaacatt cactctcata attttcacaa tattcggcct gtacagagga
   114361 ctgtggaaat atgcatccat taaagagttg ttcaatataa tagtggcatg tacaatatct
   114421 tatacattca aatacttcgt aacaaactgg atgggaataa gtcaaccccg cagtacgatt
   114481 ttcttcggat gggtactgct cataatattg gtttttgcaa caagattcag ctacagattc
   114541 ttaagatatt tggttcatga aaatgcacag cgaaaagata taaaaagaat aatggtcata
   114601 ggagcgggag atgccggcaa tatgattatc cgggagataa gatccagcgg tgtactgaaa
   114661 tccaaagtgg tttgtgtaat agatgacgat gaaaatgttc acggccttaa tattcaggga
   114721 ataaagataa tcggcggaag ggataagata aaggaagcgg taaagaaata taaagttgat
   114781 gaaataataa ttgccatgcc ttccgtgcct tcgcgggaaa aaaagaagat atatgatatt
   114841 tgtaaagata ctcaatgcac gttgcgtaca attcccggca tgtatcagat tataaacaac
   114901 gaagtaaaat tgtccgattt aagagaagtg cagattgagg atttactggg aagagatact
   114961 gtagatatta atgtcgatga aataatggat tacgttaagg gaagggtaat attggttacg
   115021 ggagcgggcg gatctatagg aagcgagctt tgcagacaga tagccggcca taaccccaag
   115081 aaacttgtaa tgctggatat ttacgagaac agcatatatg atatccagct ggagatagcc
   115141 ggcaaatatc caaaattgga tatggaagta ctcattgcaa ccataagaga cgagaagaga
   115201 ataaagagta taatgaaaaa gtacaagccg gagatagtat tccatgcggc ggctcataag
   115261 catgtaccct taatggaaac aagtcctaac gaagccgtga agaataatgt gttcggtaca
   115321 cttactcttg ttaaagcggc ggatgaaagc tctgtagata aatttataat gatatccaca
   115381 gataaagcgg taaaacccac caatatcatg ggtgctacca aaagaatatg cgaaatgatt
   115441 attcaggcat acaataagaa gtcaaatacg gaatttgttg cggtccggtt cggcaatgtt
   115501 ttagggtcca acggatcggt aattcccgtt ttccggaagc agattgaaga aggcggaccg
   115561 gtaacagtca cacataaaga tataacgcga tattttatga ccattccgga agcggtgtct
   115621 ttggtacttc aggcgggata ctacgcaaaa ggcggagaaa tcttcatact ggatatgggt
   115681 gagccggtaa agatatatga tttggccaca aacatgataa aactttccgg ccgtattccc
   115741 gatgtggata taaagattga aataaccgga ttaagaccgg gagagaaact gtacgaagag
   115801 cttctgatgg acgaagaggg aatagaaaaa acacaaaaca atttgatata cataggtcga
   115861 ccgatacagt ttgatgaaaa gatatttttc aaaaacctgg aaaagcttaa aaaaatggcg
   115921 gatgatgagg taagctgtat gaagttgctt atagctaaaa tgattccgac atataccccc
   115981 gaagtgaccg ataccacaga tgaaaatgaa gtaaacggat gcatgacaca acttcttgct
   116041 caggacaatc agattaagca gtaataaaca gttacattct gaatatataa atagcaaacg
   116101 gaggtaagat tcatgaaaac agacgacaaa attcaaaagc tcgttgacag ttacgggtcg
   116161 tggttcaaag aagataccgg attcagtgct gcaaaaatga caaacaaaca tgctccctat
   116221 cctatgttgt tttcgcccat aaagataaac agtctgacaa ttaagaacag acttgttatg
   116281 gcacctatgg gcaatatcaa tatggcggaa gaaacaggac gcccgaatga aaaaatgatt
   116341 gaatatttcc gggaacgcgc aaaaggagga gtgggcctga taaccaccgg tcttatcccc
   116401 ataagcttcg gaatagatca ttcattgatt gagttggaca aactgtcata ttttccgagg
   116461 atagaccgtt cgagaactgt ctatgtggga tggagaaatc tggcacatgc ggtgcactca
   116521 tacggggcaa ggatttttgt tcagctgaca ccgggactgg gaagggtagg aaatcctcag
   116581 tgtctgacaa atcagtttaa atttccggta tcggcatcgg ttaaccccaa ctggtatatg
   116641 gcggaagttc cgtgtatgcc gcttaccgat gcagcgtgca gaaagataat aaagaatgtc
   116701 ggacaagccg ctgccgatgc aaaagcattc ggcctggacg gagtatatct gcacggtcat
   116761 gaagggtatc tcttggagca gatgaccaat cccgcattca acagaaggaa actgggaaga
   116821 tattccgact ggcaggcttt cggtgtggaa atgatacagc agataagaaa aagagcgggt
   116881 aaatcttatc ctatcatgta cagaatagac ctttctcttg ctctcaacga aacatacggg
   116941 gagaaaatga acaaaccggc acttaagaaa tttaaaaacg gcagaacggt ggaaatgaca
   117001 ctggattaca tgaaaaatct cgtgtccgcc ggagtggata tattcgatgt ggatttgggc
   117061 tgttacgata actggtggct gccgcatccg ccggagggaa tgcctcccgc ttgttttgcc
   117121 gatgtagcaa gaatagtaaa gaagtatttt gaggaaaaca atatactttc caatgccgga
   117181 gttaaagtgc ccgtagtagc cgtaggcaaa ctgggatacc ccgatgtggc cgaggaagtg
   117241 ctgatgcagg ataaagccga catgataatg ctgggaagac cgctacttgc cgatcccgaa
   117301 tggcccaata aagcaagagc gggactcaca aaggatatag ttccgtgcat agggtgtcag
   117361 gaaggatgta taaacgagtt tgtggaaggc ggacatcccc aatgtgccgt caatcccaga
   117421 acggcattcg agcatttata cagcgaacag ccgcaacctg ctgaaaagat aaaaaacata
   117481 gcggtaatcg gaagcggacc ggcgggagta atggcggcgg taaccgcggc aaaaagagga
   117541 catgatgtaa cggtatacga aaagcaggcg gttataggag gaagacttaa tgcgggagga
   117601 aaacctaaaa ttaagtttga tatacagaat taccacgagt atcttaagaa tcttctggat
   117661 acaacctcaa aggaatacaa gctcaaggtg attctcaata ccgaagccaa tgcggaactg
   117721 ctgaaaaaag aaggatatga tacggtaatc tatgcagccg gaacaaaaga tgtggagttg
   117781 aaacttccgg gttatgagaa agccgataaa gtgcaggcgg tggatgtgtt ggaaaaaccg
   117841 gaactggtga aagcagccaa gaaagtggtg gttatagggg gaggcgttgt gggatgtgag
   117901 accgcattct tcctgaataa agaattaggc aaagaagtaa ccgtaataga gatggagccg
   117961 tacttcatga atcatgtatg tacagcaaac aggacctacc ttatctacta tctggaaaaa
   118021 gccggagtaa aactgtttaa ttgcaccaaa ctggtaagtt atgaaatagg agggataaag
   118081 gtttccagaa acatgtccaa gacggtaccc gacccgtata acacatggag cccgatactg
   118141 cctcccaata tagagaatcc tcttgctaag cccattaaag aagagttgga agaacagttt
   118201 atagaagcgg atattgttgt gcaggcagcg ggcggaagat gtgacgatac gggattttat
   118261 gctttacaga aacagcttcc gaaatgtgaa atgtacaata tcggcgactc tttcaaggga
   118321 ggaaaagtat ttgatgcaac caaagccggt tatgcggtag ggttgacagt ttaaggagga
   118381 gaagaatgga atataaaatg tttctcaccg atgaggaaaa agatattctg aacggttcaa
   118441 agggagaagt aatggccaag gttgctaaat ccgtggtcat gtacggagag gtttttgatg
   118501 caaagcgatt ggtgccggta accgctccgt ctcatcttgt aaccagtttc ggaatatcct
   118561 taatgaaagc cgtatttgcg attatggatg aaataaacga tgcgggggtt actacaatag
   118621 gcaaatttac cgtagacccg aggccgctgg attacgagaa tgtcaaatgt aatcttctgg
   118681 aaaaactggt tttcggtata atttacggaa aacagaaaga ttatgaggaa cagcttaaga
   118741 aaaccggatt aaaggattcc aatgcgttca gctgcacctg ctatatggaa gaagtaggca
   118801 atatacctaa aaaaggagat atactttcct gggcggaatc ctccgcagtg gtatatgcca
   118861 attcggtcat aggagcgagg tgtaacagaa actcgggaat aatcgaaatg ttcggagcaa
   118921 tactgggtaa agtaccggaa ttcgatttgc tgaccgatga aggaagaaaa gccacatgga
   118981 ttgtgcagat aaaaacaacc aaaaagccgg aagctcaggt gttgggaagt gccataggca
   119041 tgaaagtaat gagtgaagta ccgtacataa aaggactgga taagtttata ggcaaagagc
   119101 ttaatgcaca gacaaaagac tatctgaaag atatgggagc agctaccgca tcaaacggag
   119161 cggtaggtct ttatcatgtg gaaaatctga cgccggaagc agtggaactg aaagatgcac
   119221 ttattaaaga caaagcaaag gtttatgtga tagatgatgc cgagctggag agggttaaga
   119281 aaaattaccc tgtcatatgg aagaatgccg atgcaaagcc ttcaaggtgt tttatagggt
   119341 gtccgcatct gtcatacaat cagctgattg aatggaccga cagaatagga aaagaattgg
   119401 aaaaacaggg caagaaaaaa gtttctttgg aaacggtgtt taccgccgct cccgatgttg
   119461 tagcacattt caaagaaaac aaggaatact acgacagatt tataagttac ggcacaaaaa
   119521 tatcttccat atgccctctt atgtatatga acaatcctat ttgcggaaaa agagccgtag
   119581 tgacaaactc caataaactg aggacttaca caacggcaag atattatacc gatgaagaaa
   119641 tagccggtat tgcggtaagg ggggatattg caagatgaaa aaagaattta aaggaagaaa
   119701 tgttgtagcg ggaacggttc aaacaacagc tgtagtatca aaaaacggca tgaatacttt
   119761 ggctacattt cagaaaagtt tattggcaaa taagaaaacg gttttaggaa gcgatcagaa
   119821 caatcccgac ttatacaaaa aggaaataac cggcaaggca atatgtctgc ctaagaccat
   119881 aggctccact accgggggaa tggttctgca aagtgcggcc gctatggggg ttgctccgaa
   119941 agctatgttg ttcagtgaaa gcatagattc aatagccgct gcaggagtta tacttgccga
   120001 tgtgtggaca gaaaacagta ttgtaaccgt ggataaactg ggacaggatt ttctggatta
   120061 tgtcaaagac ggaatgaccg ttacagtaaa gcaggacgga acggttatag tagagcaatg
   120121 atacaataaa gaagaactga aaaagctaaa ggaaagcaag aagagtattt gaagaatcga
   120181 gtagggcaga attaactgcc ctctcacacc accgtacgtg ccgttcggc
//
LOCUS       DAOSOM010000004        94518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_222_length_94518_cov_5.692451, whole genome shotgun sequence.
ACCESSION   DAOSOM010000004 DAOSOM010000000
VERSION     DAOSOM010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 94518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 94518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..94518
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_222_length_94518_cov_5.692451"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="PLF14_01855"
     CDS             complement(<1..529)
                     /locus_tag="PLF14_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70074.1"
                     /translation="MVKIHKKKNKGYILPTLITLCIVLQLSACNKNSPSPYPTTTVLP
                     SATAKPTQSEVAEQTDFVQLDMLMQYQHGVQVLPAIYDTRSITLEASDGYQVKLYLIQ
                     VNGQEYPVYNGRENETLNSPLIAIPDTEGKQYLLCNQHKIYLVDLSVPVMDILLSESD
                     ETTTYRYSLENNRANG"
     gene            637..1851
                     /locus_tag="PLF14_01860"
     CDS             637..1851
                     /locus_tag="PLF14_01860"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOT70075.1"
                     /translation="MKKRTVSIFVLISLLLLMLPVQASKDSYSKHEVSAIYSKASLND
                     LQATSAILLEVNTGKILYSKEENKQIPIASLTKIMSVYLVMQAIANKEISYDTVVKAS
                     AKAHGVEGSSVYLNLGEEFTLEEMLYAIEVRSANDATVAVAEAVAGSQGDFVNRMNQK
                     AVELGMMDTYYRDCTGLTDVGHYSTVKDLSVICRALVTEYPDIFRFSTTLERDFRDKD
                     HRDYQYMVNRNNLLNYYDGATGLKTGFTTAAGYCLVGTAEKGELSLIAIVTGEPDNNH
                     RVAETAFLLDYGFANFMFTDTRDDMQTVGSIEVRKGIEKTVETYAKGTLRFLLKKTEI
                     PEISKEIVYNAATLEAPVEKDTEIGKVVYSLNGEVLGEIPVYTAEDMEKAGFWTLLWR
                     SILSWFGVDWEK"
     gene            1888..3744
                     /locus_tag="PLF14_01865"
     CDS             1888..3744
                     /locus_tag="PLF14_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70076.1"
                     /translation="MENKKKIGKILVIIITVVLAVGALLYILRGTLLNFIAPFNVTTF
                     NSDIVVKRSDGKEPLNMPYRYSKAILDNRQLYKEEIVGLNISTIKYDLSDTGISLRNY
                     EDILLNADSSEVKAAIDSIKYCKGITALSGIISDKEGSKIELYEGCTADLLLKDYKHY
                     AIIPSTFGKHINKELADKDKMFYLLNPDKNGLAYFTIIGEYEVKQDRKSNREPDTIYL
                     SYAGLSNVVRGEQEDISAHIDCMEIDVNEQADLVKFSYFLSEYFADINVISQYAKRIN
                     RFNEVYSYMFVNTVGIEPIILEEDTDFEKTVITISRIDGKENLEMSHIYGDALINEYD
                     QYSQYITDIILSTGRKGININDYPPYRAPRYGLNFGGTTHFFSDSLEYCEEQNRQFPY
                     YHQAITGLREIKGMKEGTEITFYTNYTDKDLVKVREEDIKSMSRRNNVYVKGYAILPV
                     TLHESTRNYRSIDYHVLFLSEDRMFREKPDELLYMGFKVIGYYELPEDFVPEEYKYRR
                     EDYEKQGKGIVSSLDKYGNEVKITDYEPDGYDTIYFTYAGYNDRYKLKQFKNEYIEAI
                     TIETKSDTDITQLVKYLERYFVPSDNTAKYSKEKNVLGLEYEYCYTITNKAN"
     gene            3997..4629
                     /locus_tag="PLF14_01870"
     CDS             3997..4629
                     /locus_tag="PLF14_01870"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOT70077.1"
                     /translation="MIPVMLISLTLHECAHGLMALKLGDTTARDAGRLTLNPLKHLDP
                     MGAIMMVIARVGWAKPVPVNPMNFKDPKNGMALVGLAGPLTNLLISFGAAFPVALIEQ
                     AVNLHGVEFTVFLRITYTFFYLLFQANVFLALFNLIPVPPFDGSRILFGILPDKYYFS
                     VMRYERIVGMIFLVVFFIFRSQFANVLDLIASPITKAFLFVANWFVGLFY"
     gene            4639..5628
                     /gene="trpS"
                     /locus_tag="PLF14_01875"
     CDS             4639..5628
                     /gene="trpS"
                     /locus_tag="PLF14_01875"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOT70078.1"
                     /translation="MKKKVVLSGTRSTGSLHLGNYFGAVENWLNLQNKYDCYFFIADW
                     HSLTTGYEDTDNYIANIKEVVMDLYASGLDSDKCNIFLQSKNQDHAELHLLLSMITPL
                     SWLERCPTYKDQLSQLAEKDIRTYGFLGYPCLMAADILIYRAEGVPVGEDQLAHLEIT
                     REIARRFNYMYGEVFPEPKALLTKAKVLPGLDGRKMSKSYDNTILLKEEDDVLRAKIM
                     SMVTDPARIRKTDPGHPEICSVYAFHKVFNQAEVASICESCKKGEIGCVACKKNLYGK
                     VSEFLRPIQERRREIAGKPKLMKEILEKGNANAKIKTDETMTMVRKAMKVNWE"
     gene            5619..6386
                     /locus_tag="PLF14_01880"
     CDS             5619..6386
                     /locus_tag="PLF14_01880"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOT70079.1"
                     /translation="MGVGYQLKLENFEGPLDLLLHLIDENKMSIYDIKISIITEQYLD
                     YLMAMQQLDMDIASDFLVIAATLLHIKSRSMLPKAKEDEVDSETDLIRQLIEYKKIKK
                     AGERFRELHEEGQKSLHSFASTEDFGESEKTYTLCKSMLVMIYRETMQRQIDKRNLKA
                     ENIKEIVKKEKISLIKSIRKIIDVLFKEKRISFFTRFKDNSVSNAQLITDFLSILVLS
                     TQKKATLKQKRPFEDIIVEGTETLSYPDNIQEIYDWE"
     gene            6390..6953
                     /gene="scpB"
                     /locus_tag="PLF14_01885"
     CDS             6390..6953
                     /gene="scpB"
                     /locus_tag="PLF14_01885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOT70080.1"
                     /translation="MDKEYIINQITAMLYANGEPVPLTKLEQILETDKKEIKKIIDEH
                     ITYLKDIKSGILIRQIEDSYQMCTNPIYFPAVRQLFEKKRTDTLSRAAYETLAIIAYN
                     KEATRAKIESIRGNSDSPLKTLLERGLVIEAGRLEAPGRPVLYKPAEEFYRAFDVSSL
                     DDLIPLDSFDDEEVTDTEETEVTEVSE"
     gene            6971..7684
                     /locus_tag="PLF14_01890"
     CDS             6971..7684
                     /locus_tag="PLF14_01890"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOT70081.1"
                     /translation="MRLQKYIALCGYASRRAAEELIFNGSVSVNGKIVRDIVMVDDSD
                     EVRVNGKLLKTETKKLYFVFNKPNMVITSMSDKFGRKTVADFFNNIEERVYPVGRLDY
                     DSSGLLFMTNDGDFANYLMHPKNEVTKTYAVSAKGNITDEIKEMLENGVDIGGYITGK
                     AEINNIVNYSDGKCSAEISIKEGKNRQVRKMFEAVGIKVYALQRISVGAVQLGDLKKG
                     RYRSLTQKELRDLGYERQF"
     gene            7833..9371
                     /locus_tag="PLF14_01895"
     CDS             7833..9371
                     /locus_tag="PLF14_01895"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOT70082.1"
                     /translation="MSMTEKVDELRKRKQQTSLGRGQQRIEDGKAKGKKMAADRISKL
                     LDSGSFIETDAFTQSRSTDFDMASKRADGDGVICGYGKINNRPVFIASQDYSVLHGSV
                     GEMHAKKIERTIDCAIKTGVPVIYLLDSDGARVEEGTDVLAGIGGVLTKQSMASGVIP
                     QISVVLGNCAGISSLFTAMSDFVVMADKISCMYMNGPEAILGISGVKTTDEEIGSAAT
                     HATSSGNAHIRCENEDDCINAVKLLLSYLPDNNLTEALKLEVSDAPDRTAENLYETVA
                     DSYDMREVITSVTDTSTFMEIQSEFAASMITGFARMNGQSVGIVANNPAVNEGRIDMH
                     AADKAARFVRLLDCFNIPIITFTHTSGYVADALQEKEGLIRHAAKMVYAFTEADVPKI
                     NIITGKAIGGAYIAMNSKATGCDIVFAYPTASVAVMDSEGAANIIYNEQIAKSDNPVE
                     FRKAKIKEYHERFASPYEAAKRGYVDDVINPAFTRAYVITALEMLKSKRENRPNRKHG
                     NMPL"
     gene            9390..9791
                     /locus_tag="PLF14_01900"
     CDS             9390..9791
                     /locus_tag="PLF14_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOT70083.1"
                     /translation="MKKFIARVNGKEYVVELEEVTGTPAVSAPVVTVATPVAAPVVKE
                     EVKQEVPAPKKTVSSGTAGSVRIEAPMPGTILSVKVSVGQAVKKGQAVAILEAMKMEN
                     DIVAAQDGVIVSVNVSNGESVETGQLLVSMD"
     gene            9804..11198
                     /locus_tag="PLF14_01905"
     CDS             9804..11198
                     /locus_tag="PLF14_01905"
                     /EC_number="4.1.1.112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HOT70084.1"
                     /translation="MRVQITETILRDAHQSLIATRMSTEDMKDALQLLDKIGYFSLEC
                     WGGATFDSCVRFLNEDPWERLRFIRKNVKNTKLQMLLRGQNLLGYKHYADDVVEYFVQ
                     KSVANGIDIIRIFDALNDSRNLETAMRACKKEGGHAQGAICYTISPVHTFEQYLKDAK
                     YLVEAGADSICIKDMAGLLLPYTAYDLVKVLKEHVKLPIQVHTHYTAGTASMTYIKAA
                     EAGADILDCALSPFSQGTSQPPTESIVAALRGTPYDTGLVLADINEISQIFRAVREKY
                     IKSGLLNPKVMGVDINTLIYQVPGGMLSNLVNQMKEAGKEDQLQDVLKEVPRVREDFG
                     YPPLVTPTSQIVGTQSVMNVISGERYKMVPKESKDLVAGKYGKTPVAIKQEIIDKILK
                     GEKPITHRPADDIAPALEETRREMKEYLEQEEDVLTYAMFPKVAKDFFEHRRAVKYKL
                     DKDLTDMEDKVHPV"
     gene            11333..12193
                     /locus_tag="PLF14_01910"
     CDS             11333..12193
                     /locus_tag="PLF14_01910"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HOT70085.1"
                     /translation="MSIKDRLRNTKGFSKGQKKIAEYILANFDKAPFLTATKLAAEAD
                     TSESTVVRFAIELGYDGFPDMQRDMKEVARQTLTSAERLIRISDTVDMDNVLMSVMKS
                     DIMSIRETMNMIDVDVFNTVVDKLSAAKRIYILGIRSSYSVALYMSIYFRMIFDNVHL
                     VGLPGAADVFEEIISVNNSDAFIGITFPRYSRRTVHAMEIAKNNGAYTVALTDSMDAP
                     ITEVAHNVLVARSGLTGFVDSLTAPFSVVNALIAACGIKKDKELLKKLQNLEQIWKEH
                     SIYTMENKKK"
     gene            12190..13440
                     /locus_tag="PLF14_01915"
     CDS             12190..13440
                     /locus_tag="PLF14_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOT70086.1"
                     /translation="MKKSYDVIVVGAGAAGMMAAITAADKNFSVLVIEQKKQEGSKIL
                     VTGKGRCNITNDTDSAGVMKNVMSNRTFLYSALFTFPPSEIIRYFNNIGVATVTERGR
                     RVFPKSGKAADVRDALVKQMRKSKVNVLKRNKVTSAIKGNDGFYYVNVLNDDNMTDCF
                     RCVNLILATGGMTYRSTGSDGSGYELAKKLGHTVTELKGALTGLLTKEKWVAETMGLT
                     LRNVSLTVKKDGKALYKEQGELLFTHFGISGPVVLTASRYLLDTDYRNTVAVIDLKPA
                     LEYQTLYKRITSDMEKNASKKYANSLDALLPKSLIPVIIKLSGIDPDKVCNQISREEK
                     TSLVKLLKNLTLTVTSGMDIDDAVVTKGGISVKEINPSTMESKISPRLFFAGEIIDCD
                     ALTGGYNLTIAFSTGYLAGKSVYL"
     gene            13500..13919
                     /locus_tag="PLF14_01920"
     CDS             13500..13919
                     /locus_tag="PLF14_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutP family protein"
                     /protein_id="HOT70087.1"
                     /translation="MENETIGSKDTSKAAIRLSVSSTREEEYALIKDYAEQGIKCVAV
                     DFGGDFISSVSKIIERAVVASKRQNVIRDVHHEQGAVVGATREALGQIMPKAIGCNIG
                     GKIGIARYKEHITVAVFTGIGFLNLDEVGIGLGHRSI"
     gene            13916..14581
                     /gene="cmk"
                     /locus_tag="PLF14_01925"
     CDS             13916..14581
                     /gene="cmk"
                     /locus_tag="PLF14_01925"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOT70088.1"
                     /translation="MKYKSIAIDGPAGSGKSLMARMLSEQKGYTYLDTGAMYRTVALK
                     AIRSGIDTLDGEKIAELIKNINFKLVIKDNKQYNYLDNRCVDGLIRTNQVSKGASDVS
                     KFPPVRLFLVEMQRQVAKEHNVVMEGRDIGTYVLPDADYKFFLTASNEVRAKRRLLQM
                     QQEGKTVTYEEVLADIIYRDKQDSERDFAPLKKADDAIEIDTSKMGIDEVFATLSGYI
                     NGK"
     gene            14571..15170
                     /locus_tag="PLF14_01930"
     CDS             14571..15170
                     /locus_tag="PLF14_01930"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOT70089.1"
                     /translation="MGNKCFRKFCRGIVRGIVKIFYRVEYIGEKYPEEGPYVIYPNHL
                     SILDIVFVILHSKKRWVYFMAKSELYKHKLSAWFLDKMGAFPVRRGQCDVRSIKRAVT
                     LIQEGKLVCIFPQGTRQKGNKILPPRRGVAMLSALTGAEVIPVFIERKAKIHKKTKVY
                     VGKPVNTGLDRNSSKEEIAKKSVELMDIVYNLPEVYKDK"
     gene            15178..17373
                     /locus_tag="PLF14_01935"
     CDS             15178..17373
                     /locus_tag="PLF14_01935"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF000907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase/30S ribosomal protein S1"
                     /protein_id="HOT70090.1"
                     /translation="MVAKNAGYCFGVSNAVKIAGEITEKEQIKYSYGNVIHNHHVIKE
                     LADKGLITVEDISQIPDGAGVLVRAHGVQKSFFEQAEEKNLIIYDATCPYVRNIQKRV
                     ADMYNDGYKIIIVGSAVHPEVIGINGYCDNTAVIIEQPEDAEKLLPDRENKYCLVAQT
                     TYIKEKYEKTAQELKKKFTLLEVFDTICRATSVRQNEAVKLAANVDIMIVVGDPDSSN
                     TRKLYEVCNNTCARTYLVTGAEQVRDIDIKNKETVGITAGASTPDRIIREVKETMEEN
                     AKKGSADTFEEMLEKSLVVLRNGQVVTGKVISVGEKEVYLDIGFKIDGTIPVEEFPTV
                     DGQVQVAVGDKVEAMVTRVSDKDGIVYLSKKRIDEKRRYENIYEAFKTGEPVKGTVKE
                     TTSGGLILDINGMDAFMPISQISTQFQRNLQKFVGRKLKVRVIECDRRKRKLIVSRRV
                     LIEEENERMEREVWSKLAVGETVTGTVKSFSNFGAFIDLGGIDGLAHISELSWGKINH
                     PQDVLEIGQEVEVMIRDIDKDKRKVSLVYRKEEDDPWYNAEERFAPNTIVTGKVVKIL
                     PFGVFVEIAKDIDALVHISQISNKRLKSASEVLEEGQMVEVAIIDVDLENKKINASIK
                     AVQPLDPLPTKEDLEREQLREERRQQSIKERTERFNRRVEEAESAQKSGEQRQSRKQR
                     EELPVSHEEVLSNKIGSLLADFKFEPEEEDKPKEEDKPKEEDKPKEEDK"
     gene            17505..17657
                     /locus_tag="PLF14_01940"
     CDS             17505..17657
                     /locus_tag="PLF14_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70091.1"
                     /translation="METIEQMSRENLLIVLKYREQRIKELEKEIQLYQRIIKELMEKK
                     TDNEKK"
     gene            17644..19047
                     /gene="murD"
                     /locus_tag="PLF14_01945"
     CDS             17644..19047
                     /gene="murD"
                     /locus_tag="PLF14_01945"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOT70092.1"
                     /translation="MKKNDFKNHNEYIEYLKTMKVAVVGLGIGNINLFKHLAKKGVHV
                     TGFDKNDDFTDLRKYIEEYPHASLITGPDYLDKLAGYDVIFKTQSMKRDLPQFLDAIK
                     SGATLTSEMWEFIKYCNAKIIAVTGSSGKTTTTTLIGELLKQQGYKVWVGGNIGTPLF
                     HRLDDIDVNDYVVLELASCQLQMFCNKSPNISVITNITPNHLDFHDDYDEYIYCKSII
                     FKYQKSDDIIILNQDNDITRQLRESVQSKAYMFSMTEKYDKENSINGAYLHDNAIYVN
                     IDKNIKRMLDVNDIKIPGRHNIENYMAAILATYSLVEQATVKHVATTFNGVEHRVEYV
                     RTVNGVRFYNDSIGTTPSRTVASLHCFDKKVILIAGGYDKNISYDIMGPLINEKVKGL
                     VLMGQTKKKIKQAYTDYSCTATLCEASTMEEAVRKAFNMAKEGDIIILSPASAAFDMF
                     KNFEVKGNIYKQIVNSL"
     gene            19064..20536
                     /gene="glpK"
                     /locus_tag="PLF14_01950"
     CDS             19064..20536
                     /gene="glpK"
                     /locus_tag="PLF14_01950"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOT70093.1"
                     /translation="MKKYVIALDQGTTGSRAVAFDHDGKCVGSVNKEFTQFFPSPGWV
                     EHDPYDILQSQMGALFELIKKLAAKPEQIAAIGITNQRETVVVWDKMTGKPVYNAIVW
                     QCRRTAGICEQLKADGYADLIKSKTGLVLDAYFSGTKIKWILENVKEAKNKNLLCGTI
                     DTWLIYNLTKEKSHVTDMSNASRTMLFNINTLKWDKQLLDILGIKKEMLPKVVPSSCV
                     VGHMELEGISIPIAGIAGDQQAALFGQACFEPGEVKNTYGTGCFILMNTAEKPVFSDK
                     GLITTIAWNLNNKTYYALEGSIFNSGSAVQWLRDQLGLIKTAAETEDMAFAVEDTDDV
                     YFVPAFTGLGTPHWDMYARGVLVGITRGTNKNHIARAVLESMAYQTVDVIDCMQQCSK
                     VPLKCLKVDGGASANNFLMQFQADMLNCDVVRPVMQETTSLGAAFLAGLAVGFWKDVE
                     ELKCIIKQDRIFVPKEFFDRAYRKIRWHEAVKRSKNWAID"
     gene            complement(20533..21393)
                     /locus_tag="PLF14_01955"
     CDS             complement(20533..21393)
                     /locus_tag="PLF14_01955"
                     /inference="COORDINATES: protein motif:HMM:NF019128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HOT70094.1"
                     /translation="MRLRRIITSILTIMILLSPLISINVDASAEISPQGIKYWVTNSP
                     VIYDYKNIQFKNDKLIVGGYLIVPVMEFFDFFGRYEYEWDNDTKSVTVTDGNNTYQIY
                     SGTCYLLENGHKIQSPAMNFIYNGKIYASLKVMADALGLKTLYEEVSDSILLTRHSFY
                     FNANYTYEDLYWLSRIVHAEAAHESYEGKVAVANVVLNRVRHDSFPDTIYGVIFDKAG
                     GVQFTPVAAGTIYNDPSEEAIRAAKDALNGYNNVSWCLFFFNPKLAKSFWIADTCDFY
                     KSIGNHDFYF"
     gene            complement(21552..22682)
                     /locus_tag="PLF14_01960"
     CDS             complement(21552..22682)
                     /locus_tag="PLF14_01960"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT70095.1"
                     /translation="MFKPDYTTLREKTPFTWHVNMSYYVMAELAGVTLHDLFTNYKAS
                     VKLYSKENFDKFNEFFPMHEKIKKLSPSTPPISYGHINMLGVDLAFPDGNGEVNYARS
                     DKSLDEWISILDKPIDFSVTGMVPFYLEYRDNMKKAMNIDNVNFGMAYQGPITTAYTL
                     LDLQLYYDIYDEPEKLKVLLGKLIRSIIQFKKFYCQVNNVDYKYTPYMSDDCAALIPA
                     DMWEEFVIPYMDIFYSDVNANRRSLHSEGMVYKHLKNLETLGITRYDPSVSPQLDPVI
                     IYNNCLVPFLWRMCSIYHDLLDRELAMDFVFASVRDGASGVFSCITELKEHNKNVVEG
                     FIQACEIVEDMLAKGANRADIGAMMSSRGKDIFWEKWRTQYI"
     regulatory      22887..22972
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            23077..23820
                     /locus_tag="PLF14_01965"
     CDS             23077..23820
                     /locus_tag="PLF14_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOT70096.1"
                     /translation="MANVKQTTTKQNLRSMEIYYLPVLNIQLGITQAYITQLRINDPQ
                     MGVIIDEAFMPVAERTVVTEKITTWLLDNLFNDIQRFRGRGVQADWFGVYIPTRVLLK
                     KDFLTELCKKVVEYNIQNSDLCICLYNKVLYEDHMEIKSILSQAKLEGIKSLMLDFGD
                     DFCPVSRISDIKTDYVHLSESVTESLISNDEIKRNTALGLYHLLHSLNIETITSNVKD
                     RDVSGSIPESCILCTGKFAGNYRRPRSVR"
     gene            23820..24563
                     /locus_tag="PLF14_01970"
     CDS             23820..24563
                     /locus_tag="PLF14_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70097.1"
                     /translation="MRKDLSVAKTAYNVRRTSLWFRGMVISLIALVLLLSAAYLVSAF
                     YDRYSSFTVSTAYSGSGSSGKGGSDSRGSSLLKLALSLCETPDFLTPTTRLYADAIEN
                     CTNISGQSIPENVDAINGQHNGENYLAYTFYLKNAGMEAVTYEYVVNILNVTKNVDEA
                     VRIRVYVDGTPTTYAKTKTDGTGPEKDTVEFLTESIVARNRRAYFMPGDITRFTVVIW
                     IEGDDPECLDDIIGGKFKVTMDIAIVEGS"
     gene            24624..25472
                     /locus_tag="PLF14_01975"
     CDS             24624..25472
                     /locus_tag="PLF14_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70098.1"
                     /translation="MKQIKTRHKLIPAIIMLLVAAITMSTASYAWFTMSTKVDIQGIQ
                     LNVIAPANILIRETKTLAPYNEFVNVIETTQNPTKKLKPASSVDGVAFFIPNDETAGN
                     VLASGALPETGEINTTSIPVTNEQDGYFAEYKFQLVNTGGSAVNIGLKDLQITAGGGN
                     QQDTDILPAVRFAILSEGANVITGVGEDKIFASSDTATVYALSASGSYSGSSPTTTEA
                     PARNKFDTGALFTLAANGVASAVADPDGMKDIIVRVWIEGQDTACKTTSANSTFKIQF
                     ALYIIP"
     gene            25665..26345
                     /locus_tag="PLF14_01980"
     CDS             25665..26345
                     /locus_tag="PLF14_01980"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOT70099.1"
                     /translation="MKKWLKTVGNVLLWVFIIFAVFMTVIAFTSTKNELGVSVILGKM
                     PITILTESMEPTLKKGDLIISHELKPEQKSLLKEDDIITYAVDLDKDGKMELNTHRIV
                     SVRKGGDGYIYYTTKGDNNVIADTDEVRYDNVIGVYNGRRVGGVGSVLNFLQTPNGFL
                     ICVVIPLVLFLLYEIYNFIKVMLTVKNEKKNKLDEEEIKRKAIEEYIAQQNKEQEKSE
                     SDADSAQS"
     gene            26376..27983
                     /locus_tag="PLF14_01985"
     CDS             26376..27983
                     /locus_tag="PLF14_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002852337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70100.1"
                     /translation="MDAFLGWFFEFMNYMLRGVIRFFVGIYEGIVQTFNIVKYIEIFR
                     SYYQQFKFLDWFFAILAIILVIAVWLTIILLIILGIKRIINIRKNTVTQESIFEEITD
                     LHREILRLNDEKDRILSLKISQAGISAKDLAKELTDIAEKSREKVSQMDEFGTATTDS
                     TGDISVSSDQQPEVMMESRFSKLIEVDQKYTYYTPQEYTTGFTLEQLCNDFRNFACSK
                     MKLYYELEMMRVLISSMASTKLIVLQGISGTGKTSLPYCFGKFIKNDVTMASVQPSWR
                     DRTELFGYFNEFTKKFNETEVLRRIYEASYNDDINMIVLDEMNIARIEYYFAEMLSVL
                     EMPDPEQWQLELVPSSWTSDPRNLVGGKLRIPENIWYLGTANNDDSTFALSDKVYDRA
                     FTINFESKGQPFEAPDTEARPISFTYLEELYSDAWERFPVSKENLDKIAKLDLYIIQK
                     FRIAFGNRILKQLLEFVPVYVACGGKEIDGIDYVLYTKVLRKFESLNLSLIRDDLKEF
                     VTYLNRSFGRNNMNECKKYLELLQRMY"
     gene            28007..29248
                     /locus_tag="PLF14_01990"
     CDS             28007..29248
                     /locus_tag="PLF14_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70101.1"
                     /translation="MARRKKKLILDQEQKDSIYEDTKDFISYIYEDYDFYQSLVRLLD
                     RGKNQAVLYRKVLQKNIDEIWVKRVEDALPYLDTALRAVRKDIKEVEEIKPIALSRNI
                     TVKSLRHLATHTNFIDRVDEKGNVIPNKLLNVYKDEEILTYENKFLNTLIYKLALFID
                     VRYEKLKEYGADEVSNVMSMDIETKVVDTELKMSIHIETKDGADEQDKKDNVDIYEDV
                     SKSSLWARVLRIRKIIRQYQASPFITQMGKAYIRPPVMRTNAITKNVHLRECLELWLF
                     IESYESAGFDIESSSVTEKPSKELMENLYRLLAFQYVMFRKNTYDQLDDIINKSTAKL
                     RYNARFKQLDTQEFTDQYEFLTPGVKKVYVEELSRKGTRLTKEEVKIRKAIDNALKSE
                     RLYAAERKKAAPKQRRKTKQK"
     gene            29287..29967
                     /locus_tag="PLF14_01995"
     CDS             29287..29967
                     /locus_tag="PLF14_01995"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOT70102.1"
                     /translation="MKKLFKRKNIRTIFTVVFSVLLVLLVLLVGNIIYSQITHKVPAI
                     FGYSVMNIIAGSMEPVIPKNTFILIKQVDPEQLQVGDVITFYSSDPLIQGLPNTHRIF
                     DIVMENGQKFFVTKGDANAIPDSYNVGFDDVIGVYVKNIISLGKIGMIFQNKSVVFLL
                     LVVPAAVLFIMEIINLTKTAAKVKQEDIESASIEEKCDDVETSDSKETDSEDIKSEEK
                     EEKSVEST"
     gene            29954..30607
                     /locus_tag="PLF14_02000"
     CDS             29954..30607
                     /locus_tag="PLF14_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70103.1"
                     /translation="MSQPKNAENVIEEKDEDIIEEKDVETEQKADAVEEQDVKKKKKG
                     LIERFLGPKEPDVNDDSDYQSVLRKNFYDFDQINKKRQITSLRMAVMALTLILIVILT
                     LYIVHLSDSTILLDKLIDSSSKYVLDNVMYVDNPIKYTEVVSELNVMRTLMNHNGELG
                     KRQYAISELYYIAIQLPDSFVKYKAEIYDAFKNINEGKQDVGYKLIEELLVKIVEER"
     gene            30616..32883
                     /locus_tag="PLF14_02005"
     CDS             30616..32883
                     /locus_tag="PLF14_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOT70104.1"
                     /translation="MRRGVSFKSAYLVPSMIIIMFLLLFVMLFQMRFSITEFVMEQAA
                     TDNETIAKEKSALFDSLLVNMSLETNGILNYTDFTFYTIENEYDAREAISDYFPNTNC
                     VRFFYFMRDGRVYDQDTRLYKDIEDNLAQLKATETLSLSMPFYDEAAKTELIIIYLPN
                     PDSGSVLAGVAAYYKVSEIYTIGEFYVSDTGSHYIFDKDGNSVFLKDKGITVASLMES
                     YEFPETDINRITNFMQEKGIVDTVISSSDSAVISVCGVRKFVDWYVVSLLPSTVISDK
                     TSHVIEQTLLISVLILAVFIIFVIIFLAADTRSLRKMERLQNIDPVAECANQYKFEID
                     AGEILRANSSTKYAILYINIMNFKNIKQLLSVSEVNAIVRNLSDILRKTIVRNLETYG
                     RTSDGRMVVLMRYKDIEEIQDRFNEIAEDFKSTDPVSGYMMRLNAGAYCIDRSGNYQV
                     SEMIDRAAFAQNSIKEDSNISLDFYHQKSHDDLLRTVALEGFMEKALSNDEFIIHYQP
                     KFDLNNNRIYGAEALVRWVRPNEGIVPPSQFINLFERSGYISQLDRFVFTEVCKFQNR
                     MVKEGSRIIPISVNVSRASAEKEGFIEYYVNTKNQYGIKDGFLELEFTESKALEDNDL
                     LSDRIKELKKYGFAISIDDFGAGYSSFKALQTLDFDIIKLDAVFLKTNPNNPKKNVNL
                     LSGVIALAKTLGMKVIAEGVETHQEVALLRELKCDVIQGFIYARPMTGTDFVAFVKNA
                     LSTRTEKDMLTEDIT"
     gene            32885..33469
                     /gene="rsmD"
                     /locus_tag="PLF14_02010"
     CDS             32885..33469
                     /gene="rsmD"
                     /locus_tag="PLF14_02010"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOT70105.1"
                     /translation="MPRIIAGSRKSIILEAPKGENVRPTYDRTKENLFNIISSRYPLE
                     GAYVLDMFSGTGSIGLECLSRGAKYAVFIDKDVSLTRKNIEKCDFSDVSRTVRGDWAT
                     GLRILKEENIKFTHIFLDPPYHSGLHERIIENSDLYDIMGENGVVIAELSDKANVPEN
                     TAELVLKDKRKYGICVFAFYIKGSHEDLDMSGEL"
     gene            33438..33917
                     /gene="coaD"
                     /locus_tag="PLF14_02015"
     CDS             33438..33917
                     /gene="coaD"
                     /locus_tag="PLF14_02015"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOT70106.1"
                     /translation="MKIWICPGSFDPVTKGHIDIISRAGVMCDKLIVAVGENKFKKSV
                     FTAEERVDFIRRSLNGLKNVEVESFHGLLIDYAREKKAICIVKGLRAVSDFEYEMQMA
                     FLNKRMDEEIETVFLMTNINYSFLSSSAVKELALHGGPIEGLIPDAIIDDVRKKFFK"
     gene            33934..34320
                     /locus_tag="PLF14_02020"
     CDS             33934..34320
                     /locus_tag="PLF14_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70107.1"
                     /translation="MDNTMNEYEIIEQMEDLVTSAKQTVFSDRISISKDEMLDLIYYL
                     KNAIKQRDQEEEAKNKELLRMISEAKNRESDADDQSVVLEDANREANEIKRAALKYAD
                     NLLASVQESIEKTLNIIASNREELDQ"
     gene            34493..35095
                     /locus_tag="PLF14_02025"
     CDS             34493..35095
                     /locus_tag="PLF14_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004061280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70108.1"
                     /translation="MNKAIAKAGSIIVTLTVILFAIFMFTSEFASYLVCIFLALGYLM
                     MIAGYHNESSDDRKAAANVGLIFGCIYAVFIILVYFAQTTAVRTDSLNEQAIMILDYS
                     KGGLFFSYDLLGYGMMALSTFFIGLSIKPKNKTDKWLKYLMMIHGVFFLPCLIMPMTG
                     VFGSFMADDSSSIGGIIALEFWCAYFLPIGILSFFHFKND"
     gene            35192..36592
                     /gene="miaB"
                     /locus_tag="PLF14_02030"
     CDS             35192..36592
                     /gene="miaB"
                     /locus_tag="PLF14_02030"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOT70109.1"
                     /translation="MNDFCNKYPSDNMLLYNADQYCRKIASINYGKNAKYFIETLGCQ
                     MNEADSEQLAGMLTDMGYSQAEDISSADIIVINTCCVRENAEFKLYGHLGELKILKQR
                     NKELLIVLCGCMMQQSHVVDKIKNSYKYVDILFGTHNRQEFPELLFDSISEKKKIYEI
                     WEESRGIAENVPVKRSSTITAYVPVTHGCNNFCSYCIVPYVRGREHSRHPEIIKKEII
                     QLASEGIKEITLLGQNVNSYYCGMDFPGLLHYISDIEGIERIRFLSCHPKDLSDDLIK
                     AMVEIPKMCSHIHLPLQSGSNKVLQQMNRKYTKEQYLMLIDKIRASIKDIAISTDIIV
                     GFPGETEEDFNETLEVYEKAGFDFAFTFIYSKRTGTAAAKRPDQIPEDIKDTRFARLV
                     ALVNDMTLASNIRDENKVLQVLTGGKSRTDRSVMTGRTSSNKIVNFKCDNDFTGHLVD
                     VKIKKGHTWYLEGEKV"
     gene            36589..36981
                     /locus_tag="PLF14_02035"
     CDS             36589..36981
                     /locus_tag="PLF14_02035"
                     /inference="COORDINATES: protein motif:HMM:NF017902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HOT70110.1"
                     /translation="MIFDKVDELSALIEDTKQYDDYLEAEVELENDGHGIDMINAFYD
                     LQQEYIKTYNEGSYDEILAMENVLEMRHTELMNYTTTGNYIRAKEALDELLQQITDRI
                     MSNLGLDTDCDDDCDCDCDECSYGGHIN"
     gene            36982..39546
                     /gene="mutS"
                     /locus_tag="PLF14_02040"
     CDS             36982..39546
                     /gene="mutS"
                     /locus_tag="PLF14_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOT70111.1"
                     /translation="MENLTPMMKQYMKTKEQYKDCILMFRLGDFYEMFFDDAVTASKE
                     LEITLTGRNCGLDERAPMCGVPYHSVDPYIAKLIKKGYKVAICEQLEDPATAKGIVKR
                     DVTRIITPGTVHENTLLDEKENNYIMSLFKRGEVYSLAVCDVSTGELNSCQIIYGNTL
                     EKLINEIVKYKPKEIISNFDTEFIKQYISVYVSDVEQSLFDRSFPDIAIKSKSKTNDF
                     DIWRNACAALYQYLKETGKCDISHISFVNVYEAEQYMSLDLNARNNLELTENLKDNRR
                     YGTLLWVLDKTSTAMGARLIKKWIEQPLNDIDDINLRLDSVAELKDKYIMRQELMELL
                     KSVYDIERLVSKTVMANANGKDLISLKNSLFKLPYIKQLSVKLESDLLRHTDSNLNTF
                     SHLADLIEQSINEECPVVLREGNLIKSGFDKGVDELRKASHGGKNWIIEFEEKEKERT
                     GIKKLRVSYNKVFGYYIEVTNSFLDQVPTDYVRKQTLVNSERFITQELKDMEESILGA
                     EEKLIRLEYEIFCKIRDIIASDAEKLLKTAYSLSVLDVLCSLSEAADRFTYNRPEMTK
                     DCVIDIKEGRHPVVERMPTCPEFVPNDLYINDTTDKTLIITGPNMSGKSTYMRQAALI
                     TIMAHIGSFVPAQSATIGITDKIFTRIGATDDLAGGKSTFMMEMSELAYILENATDKS
                     LLILDEIGRGTSTFDGLSIAWAVIEYLNETRALRTLFSTHYHELTVLEDKLSGVRNYF
                     ISAIKKDKDIVFLRKVKRGAAKESYGIEVARLAGIPEQLVVQAGEFLTELEKSENKRT
                     RIRNKETYMEGQVGILEFNKRDDNYKEIKDILENCDIQKMTPLEALNKLYELKSKV"
     gene            39548..41350
                     /gene="mutL"
                     /locus_tag="PLF14_02045"
     CDS             39548..41350
                     /gene="mutL"
                     /locus_tag="PLF14_02045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOT70112.1"
                     /translation="MSGKIIILDEFTSNQIAAGEVVERPASVLKELIENSIDAGADKI
                     EIEIKRGGIGYIKVSDNGKGIEPEDIPMVFERHGTSKIRQISDLTTISTMGFRGEALP
                     SIASVSQVTLTTKTQDNEYTTKAVYEALQQKSIVKAARAAGTTVEVRNLFYNTPARYK
                     FLKGNTQEARYCTDIVLRLALANEHVAFTMISDNREVLRTTGDKDLLNTIYSVYGRDI
                     AKDLIKVDAKSDTFSVAGYIGKPFASRGNRQHQSLIINNRYVKYPELNAAIERAYQTH
                     VMKGRFPFYVLKLNVPAHNLDINVHPAKTEVKISNISDIFGKLNFIIKEALDKEQQAF
                     TPATVKPSYTKPEIKKEDGNLIKDFTEQLAKLREETPPVTPKTHIINEQITVYKEQPV
                     VNTSDIKQEFTDSVADKIYYDEPYKIIGQVFNTFIIVEKTDYIYIIDQHAAHEKMFFE
                     SLKADYDKQSVISQQLMVPTEVVMTSVEMSFLEQNSELIKRMGFDYSQFGKNSILIRE
                     IPSTINECDIKDAFATMLDDMQSEKKDKVNTAIYSLACKAAVKANRKLDEKQMRYLYE
                     NLMKLDNPFTCPHGRPTIVKMTRYDFDKLFKRIV"
     gene            41350..42411
                     /gene="miaA"
                     /locus_tag="PLF14_02050"
     CDS             41350..42411
                     /gene="miaA"
                     /locus_tag="PLF14_02050"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOT70113.1"
                     /translation="MKKVIIITGPTASGKSKAALSLAKKIDGEIISADSMQVYKRMDI
                     GTAKPTKNELDEVKHHMIDICNIDQDYSVAQYKEQATDSINDIIYRKKTPIITGGTGL
                     YLDSLIYNIDFKQERQSNREYYEQYLKNNGKDSLYNLLLQRDPDAALSTHPNNTKRVI
                     RYLDILSGFEGTLEEYKKAAIGKNTDFDFHIYLLHMQREQLYRRIEKRVDEMLDAGLV
                     EEVKGILNDGYSTDCNALTAIGYKETAEYLRGFCTYDEMVNILKKNTRNYAKRQITWF
                     KRYEEARIINIRDYIDADDIAEFIREDVMRTMLKEEKNEKVSPVAKITGILEGKVEPN
                     VDKEDIKNERLSERYVKKS"
     gene            complement(42398..43000)
                     /gene="lexA"
                     /locus_tag="PLF14_02055"
     CDS             complement(42398..43000)
                     /gene="lexA"
                     /locus_tag="PLF14_02055"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOT70114.1"
                     /translation="MKDYLSIREEQTYEYIRDYIREKGYAPSVRDICRDLNIPSTSTA
                     HSYINKLRRLGYIEKNDSISRSLTLSKQDAVPVVEVPVIGDVAAGTPILAVENIVEYF
                     PVPEAYLKSGITFMLKVKGDSMVNAGIHDNDLVLVKKQEVAYDKDIIVALIDNETTVK
                     TFYRERDRVRLQPENDNYEPIYVYNDLQILGKVFGVFRIF"
     gene            complement(43166..44899)
                     /locus_tag="PLF14_02060"
     CDS             complement(43166..44899)
                     /locus_tag="PLF14_02060"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HOT70115.1"
                     /translation="MNRKISYKLITVLITILCLLTICGCSNQTEEDNDIFDYIIEDSS
                     VMITKYKNMEGTEKVVIPDTINGYPVKGIDNEVFMNMTDIINITIPDTVSHIGYNAFK
                     GTKWYENLNDKFVIAGDKVLIKYNGTEINVEIPKTVKYLSACAFTDRNDIWAIKLHEG
                     ITIIPDYCFKDCLALKSVYPIEQVNYIGESAYEGTSIGDDFIIPTGVDTIHKKTFYNC
                     KGLLRILLHDNIHTICEQAFAGSSLYEIRINGYDNEQRDYQYGTANSIVLPKNIKYVA
                     DDVFMGCESLKSLSTIYVREGIESINKKDFTILEKPGYVYIPLSVKYIEERMFDAYSD
                     ITLLVYENSYAHEYAKNNQYRYILRPLFADALFERILKSGFREKGWEFTHENMLKVES
                     ILIYPSFFNHNDIEETPYILTINEDGPCGGVENDRITRITSLEDLKYFENLTSLEIVS
                     LTKISDISPIVNCPNLKHFTVRYANIRDISPLLQLKTLNWLGLDNNPIEDFSPIGQLT
                     DLTLLTLNNTGVKDLKFLENLTNLEVLDLCNNQFDDVTPLLSLKRIRHLYLDEQKLKD
                     YNNLKKTNYAD"
     gene            45080..46345
                     /locus_tag="PLF14_02065"
     CDS             45080..46345
                     /locus_tag="PLF14_02065"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOT70116.1"
                     /translation="MKKTILSLNCGSSSLKYNVFRINNDLTVTPILNGNAEEIGNPQR
                     STMTFKINDEKNKYPVPLETHEDAIHEMIKVFRKNGITKESICAVGHRIVHGGPVYTK
                     STLITEEVKKQIIELFSVAPLHNPQNYIGVEEIEKLLPGVPNVAVFDTAFHGTMGEEA
                     FRYAVPEKWYKEFLVRRYGFHGTSHLYVSKRAAVLLKTPYNEFTCITAHLGNGSSITK
                     VENGKCRDTSMGFTPLAGVIMGTRCGDIDPAIIGHVAAKYASEGMDKYEAFEKTVKML
                     NKESGLMGIAGTNLMQDIRAKALEGDEQCMKIVDIYCYRIAKFIGEYACTTDNLKAIV
                     FTAGVGENEWFIRQRVLEMLKAFAFEIDNEANRLRGEEVIVGKGRFAGNDVYAMVIPT
                     DEELVIAYDTLAIGYLGEKAPTVYPFEKV"
     gene            46332..47501
                     /locus_tag="PLF14_02070"
     CDS             46332..47501
                     /locus_tag="PLF14_02070"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HOT70117.1"
                     /translation="MKKFKTKDNFNRFFEKHLSVIDLIHECGYKAYFIGGCVRDAVMG
                     VQASDFDIATNATPEDIINMFTDVRKTGIEFGTVTVKTATGSYEITTFRKEYGYSDNR
                     KPDKVVYAKSLYDDVRRRDFTVNALAYDGKYVYDYFNGIEDIQRKIIKTVGSPKRRFS
                     EDALRILRAARFASTLDFSIEDKTSESMKRCAHLLRSISKERIFSELSKMIAGGESLK
                     ILFDTGIADYIFLYPHHINQDNLKKGSYKCRLADVFCKYPSADMAVEELKNLRADNKT
                     VGTLYDILIFLNSDTDEMSVRKLLSEIGLDNVSVLLEFSGQSRDVLDKIVKEGYITGI
                     NELAISGHDIMAMGYPKESIKDIKWFLFNKVIYDISLNKKEILQDLLNNCPIEKH"
     gene            47545..49347
                     /gene="lepA"
                     /locus_tag="PLF14_02075"
     CDS             47545..49347
                     /gene="lepA"
                     /locus_tag="PLF14_02075"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOT70118.1"
                     /translation="MKKDRIRNFCIIAHIDHGKSTLADRLIEKTGLLTSREMSDQVLD
                     NMDIEKERGITIKSQAVRMVYKHNDGKEYILNLIDTPGHVDFSYEVSRSLAACDGAIL
                     VVDAAQGVEAQTLANVYMAIDNNLEILPVINKIDLPAARPELAIEEIENLIGIEAHHA
                     PLISAKNRINIEEVLEKIVTYLPAPLDKSDMPARALIFDSKFDAYKGVIIYCRVMEGV
                     IKATDTIRLMNTKKEYQVVELGYLKPGGFIPAEQLTSGEVGYITASIKNVEDTRVGDT
                     VTTAENKAAVPLPGYKEAVSMVFCGIYPADGAKYNMLKDALMKLKLNDAALMYEPETS
                     AALGFGFRCGFLGLLHMEIIQERIEREFDLDIITTAPSVIYRVTKTDGQVLRVDNPTN
                     LPPVSEIAYMEEPIAKAVIIVPAEYVGNIMELCQDRRGTFIHMEYIDATRTKLDYELP
                     LNEIIYDFFDALKSRSKGYASLDYEVMKYEKSKLVKLDILLNQELVDALSFIVHESKA
                     YARGRRMVEKLKEQIPRHQFEIPVQAAIGGKIIARETVRAYRKDVIAKCYGGDISRKK
                     KLLEKQKEGKKRMRQLGSVEVPQEAFMSVLKLDT"
     gene            49349..50470
                     /gene="hemW"
                     /locus_tag="PLF14_02080"
     CDS             49349..50470
                     /gene="hemW"
                     /locus_tag="PLF14_02080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOT70119.1"
                     /translation="MNDLRIYIHIPFCKSKCNYCDFNSFAGMDHMFRPYFKSLITEIA
                     LYSNELRGRRIDSIYFGGGTPSHVDVQYIYQVLKCFTFDENTEITIEVNPGVVGYHNF
                     VQYRQMGINRISFGIQSFNDRILMYMGRIHNSLTAINNIHECKEAGFDNISADMIFGY
                     PKQTLEQYEDSLNVLKSLEIPHVSCYSLSIEPGTLLDEMVKKNVMPMPDEDLDRAMYN
                     LSIESLKKANLQQYEISNFAVPGYESRHNIGYWNRDEYLGFGLSAHSLYNETRYSNTE
                     DLHQYISLLNKYEKPVVHTDTLTKEEIITEYIILKLRMNSGLDIAHFQDKFDLNFEEK
                     YGDKLKTLVNTGLLYKQSDSYKLTRSGMDVANKVFVEFV"
     gene            50593..51453
                     /locus_tag="PLF14_02085"
     CDS             50593..51453
                     /locus_tag="PLF14_02085"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HOT70120.1"
                     /translation="MHITITGMLGSGKSTVCRIISEQTGYEVYSTGKIQRQVAAQKGI
                     STLELNKMMSDNTELDNIIDNETVRISDAVKDRIILFDSRMAWFFVKQSYKVFLTVDP
                     LVAAQRVAKADRGGVEDYTDVNEAKQKLLERAAAEHERFIKIYGADYWDYNNYDLVLD
                     ATWHDPHTLASIVMGQFAMFTESDEQQSAILISPKSLYPVFMEGKKQPDKQSWNAANV
                     RIVADNHYNYVIKGHAVVLRALENRTEFISVLYNKDIKTDNLVVHKDSLSLFEKYGEF
                     KYKSLPDNVI"
     gene            51563..52588
                     /gene="hrcA"
                     /locus_tag="PLF14_02090"
     CDS             51563..52588
                     /gene="hrcA"
                     /locus_tag="PLF14_02090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HOT70121.1"
                     /translation="MDKRAANILLVIIEEYVNSAEPVGSKSITEKYPLGVSSATVRNE
                     MHKLEEEGYITSLHTSSGRIPTDKGYRFYVDYLTEHIRQREIEEKIYKLLKKVEIEET
                     LGYILERLFLGNPYTSLGVLKRNVSRIDTFHILSVAKDTYLVLVIFRNGVAAHRLIKE
                     QLSDKVQVNILSEYLNEKLYGMTLRDIQTTDFAFIDTFDRDNKAFFIRVVSEVMDILH
                     QYEDNEFFLKGKENLLSFPDFKELSEVKSIFDAFSREMELSELLLCPTDDAFSVSIGN
                     EIKNENFNKLSIAKHTYDLGAEGKITFAVAGPTRMDYRRVMNSLNYMETILGDIIYDK
                     NDKKEVQ"
     gene            52588..53121
                     /gene="grpE"
                     /locus_tag="PLF14_02095"
     CDS             52588..53121
                     /gene="grpE"
                     /locus_tag="PLF14_02095"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOT70122.1"
                     /translation="MAKKKEEKDVNIQQDGAEVSDESNTGNEEVTSCETDIEKENREL
                     TDSYKRLAAEYDNYRKRTAKEKQDIYDICVADITEKFLPVLDNLDLALANCDSSDDNV
                     VKGIELIHKQFLEILAKLDVFEIDCLNKPFDPNIHDAVMVTEDPDIQPGTVVEVFKKG
                     YIYKDRVVRHSVVKVAQ"
     gene            53185..55029
                     /gene="dnaK"
                     /locus_tag="PLF14_02100"
     CDS             53185..55029
                     /gene="dnaK"
                     /locus_tag="PLF14_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOT70123.1"
                     /translation="MAKVIGIDLGTTNSCVAVIEGGEPVVITNAEGARTTPSVVAFSK
                     TGERLVGQVAKRQAITNPDRTIVSIKREMGSDKRINIDNKKYSPQEISAMILQKVRAD
                     AEAYLGEKVTQAVITVPAYFSDSQRQATKDAGKIAGLDVLRIINEPTAAALAYGMDKD
                     QDQKILVFDLGGGTFDVSILDISDGVFEVLATNGNTRLGGDDFDNKVIDYLAETFKND
                     NGIDLRNDKMALQRLKEAAEKAKIELSSVLSTNVNLPFITADATGPKHLDITLSRAKF
                     DELTADLVQKTMDPLRKAMNDAGLKPADINKVLLVGGSTRIPAVQDAVKKFIGKEPFK
                     GINPDECVAMGAAIQAGVLTGDVKDLLLLDVTPLSLGIETLGGVCTRLIERNTTIPTK
                     KSQIFSTAADGQTAVDIHVLQGEREMAQYNKTLGKFQLTGIAPAPRGIPQIEVTFDID
                     ANGIVNVSAKDLGTGNEQKVTITASTNLSEDEIQKAVNDAKAHEKEDKLKKEEIDARN
                     NADSLIYQTEKLLKDNADKVDPTEKAKVEEQIENLKSALKGTNVEQIKTATDDLQQSV
                     YKLSEGMYKQAGPDMGQAGPDADSHTTDDHTKDDNVYDADYEVVDDDK"
     gene            55117..56226
                     /gene="dnaJ"
                     /locus_tag="PLF14_02105"
     CDS             55117..56226
                     /gene="dnaJ"
                     /locus_tag="PLF14_02105"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOT70124.1"
                     /translation="MAEKRDYYEVLGVDRNATDEELKKAYRILAKKYHPDVNDTPEAE
                     TLFKEIGEAYSVLSDPDKRRQYDRFGFDGLNGAFSSGFAGGFNPFDIFNDLFGGGFGS
                     GFSSGFSRRNAPMRGRDINQSIKISFEEAAFGTKKTITVQRSESCEQCEATGAKDGKA
                     IKTCPQCKGTGEMRVQQNTLFGQMITATTCNKCNGKGTVIEELCEHCKGAGTIRVTKK
                     IEVNIPKGIDNNQIISLRGQGDAGKNGGPEGDLRVHITVTPHEIFTRENTTVYCTIPV
                     TYAQAVLGAEITVPTLDGDIKYNIPAGTQTGTTFTLKNRGIHFLNKESRGDQIFEVYV
                     DVPKKLNREQKKALEAYAALFGESTDKDKKKKRLF"
     gene            56226..57158
                     /gene="prmA"
                     /locus_tag="PLF14_02110"
     CDS             56226..57158
                     /gene="prmA"
                     /locus_tag="PLF14_02110"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOT70125.1"
                     /translation="MEWIKVKIFTDKDNQEELQWFLINMGINGCTVEDSADFNDFLKD
                     KSFNWDYIDNSLMKWKDIRSSITVYLADNEQGNMQLDCIKNAYTDIETEKISDQDWAN
                     NWKQYFKPIEIGKRLVIRPSWEQYDNTDNRRVVTLDPGNSFGSGTHETTKLCLMQSEA
                     LVKEGFAVLDAGCGSGILGISALVLGAKSCVFFDIDQASADTTMANAIANDVQDKSLV
                     YCTDILEDMESYSLLLAKGPYDMVFANIVPDVIAAMLPFLSKAVKSDGYLICSGIIKE
                     REKETADNMRKYGFSSIECNELNGWVALTGRKDA"
     gene            57151..57957
                     /locus_tag="PLF14_02115"
     CDS             57151..57957
                     /locus_tag="PLF14_02115"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOT70126.1"
                     /translation="MHRFFVQSKLNNNMTVRIEGDDYQHLKNSLRIRTGELIEICDGC
                     NNTFIASVGNLNKNYCEAVLQERIPLSNEPGCKITLYPAVLKGDRFESMIQKCVEGGV
                     YAVKPVITSNTIVDISSENAVKKHKRWSRVCKLACMQSKRDILVNIDKPVKLFDAVMC
                     LQSGNNLNNNDSKKESKRYRKEISFVCHEAEEEHSIDAILEKAFGEKVENINIFIGPE
                     GGFSEQEIEKLNEYGILSVHMGKRILRADTAAYSAVFYTAMRYELNRVNQ"
     gene            58008..59840
                     /gene="htpG"
                     /locus_tag="PLF14_02120"
     CDS             58008..59840
                     /gene="htpG"
                     /locus_tag="PLF14_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOT70127.1"
                     /translation="MKEKGNISINTENILPIIKKWLYSDKDIFLREVVSNAIDAVTKH
                     KKVSSLGESEQPEKYRIDVKVNKKNKTVTVSDNGIGMTFDEVKQYINQIAFSGAIDFF
                     EKYKDKTDATDQIIGHFGLGFYSVFMVSEYVEIQTKSYIESEQPVYWKSDGTSGYEMS
                     EGTRQTHGTDIIMHINSDKENKDLVNVWKMREILDKYCKFLPVEIYLSEEGAKKDKDG
                     KDASEEKPVNNTSPLWLKDAKDCTDEEYKAFYREVFRDFKEPLFWIHLKMDYPVNLRG
                     ILYFPKLTNEYNTLEGKIQLYCNQVYVADNIKEVIPEYLLLLKGVIDCPDLPLNVSRS
                     FLQNDGYSGKIAGHISKKVSDKLKQLYKSDAEQYYKYWDDINIFVKYGCLKEEKFYDK
                     VKDIIVYKDIYDKHHTLEQYKKINEEKKQKDVIYYVSDMQKQAQYINIFKEHQQNAVI
                     LDNILDSHFLTFIESNNPGTSFMSIDSGIKDVLAEEGATEGFDDIKKFFLELLKDKKV
                     SVEVEALKSSDVSAVIISDEYESRMKNMQTMYGMEFPSSNTLVLNTRHPVISKLATME
                     DEQKKSDIAIYIYQLAMLSAGKLEKEDMTDFLKRNNEMLNKSTE"
     gene            59864..63655
                     /locus_tag="PLF14_02125"
     CDS             59864..63655
                     /locus_tag="PLF14_02125"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HOT70128.1"
                     /translation="MRSVRTVGNIRRIYIERKSGFDAEAKGLLDQCVNLLGIKKLEHI
                     RILNRYDIQGMDDSIYAVAKYGIFAEIQTDTVYEEEFDAKDAAYVFAYEFLPGQYDQR
                     ADSTEQCIQLISHTRPIVKTAKVVCLYGNVKNEDIEAIKKYLINPVECRIISIKKPDN
                     LTEHFEVPQSVDTVTGFITFHSSELKTLKQQYSFAMSDEDLLFVQQYFRSVHRDPTIT
                     ELKVLDTYWSDHCRHTTFNTVLSDITIEKDKYNETCENTYKRYLEIRKQLYKGKEKDI
                     SLMDIATIGARYLKSKGLLDDLEDTKENNACSIVVEAKVNESLEKWLVMFKNETHNHP
                     TEIEPFGGAATCLGGAIRDPLSGRSYVYQAMRVTGAGNPFTKIEDTIPGKLPQIKIIR
                     EAANGYSSYGNQIGLATGLVDEIYHDSYVAKRMEVGALIGAAPLKNVKRCDPADGDVV
                     LLIGGRTGRDGIGGATGSSKGHTEQSLESCGAEVQKGNATIERKIQRLFRNRKMSLLI
                     KKCNDFGAGGVSVAIGELADSLDIYLDKVPKKYEGLDGTELAISESQERMAVVVDKKD
                     VDKALKLAEEENLEATPVAKVTDSGRLRMFWNGHTIVDIERSFLDTNGVKQYAKAYIA
                     GKDENNNPFYLKTRGDKSYKERLFESVTDLNACSKKGLIEKFDSTIGSATVLMPLGGK
                     YQITPAEVMAAKLPVLNGKTNYCTLMSYGFFPQIFEWSCFTGACYSVLSSVAKIVAAG
                     GDYTNIRLTFQEYFESLRKDPVRWGKPLQAVLGALHAQLELKMPSIGGKDSMSGSFMD
                     MDVVPTLISFAVSHTDAENVLSPEFKKAGNTVIRFSHRQKADSTPDFADSLKIFAAFK
                     ELNQKKAILSASTVKHKGAAEALLKSCFGNMIGFELSADVNTLFTPGYCDIIAEIDSD
                     KIEDIEISYIFDKYKEVDISVVGKTIEDKQFVMGNLSVALSELVEAYTDKYERVFPTK
                     AKTNTPKINNTLYDGKSIHYSKNKIATPYVFIPVFPGTNCEYDTKRAFEDHGAVCDFS
                     VFRNLTPAHIDDSVKDFVKKINKANIIAIPGGFSSGDEPDGSGKFIATVFRNPYIREA
                     VYDMLYNRDGLMLGICNGFQALIKLGLVPYGKMVELKENSPTLTFNSIGRHVSCMVNT
                     KVVSKLSPWFMCADLGEVYTIPVSHGEGRFICTKEEYLNLSKNGQIATQYTDDYNNPS
                     YDIQYNPNGSYYAVEGITSPDGRILGKMGHSERIGQYICKNIPGNKDQMLFASGVKYF
                     R"
     gene            63672..64709
                     /locus_tag="PLF14_02130"
     CDS             63672..64709
                     /locus_tag="PLF14_02130"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT70129.1"
                     /translation="MISKTVIKKVLEFDNPGRIGYDFLDGSLRDFKFVELNIPVKEHN
                     KWQKPAYFADMYPEYKDFDGTLRLDEYGNLWGRMAHDTTGGGEVLEGALKSWEDLDSY
                     KLPDIDDEKRFFNAHLSVEPYKERFILGPIPGCTFAIMRNIRKMEYFLEDLVLEEDNV
                     LKLQDMVITKLVNMVDMFGAMKCVDGIFFCEDWGIQDRLLVSPAMWREIFKPAFVKLC
                     TAARRNNLKVLMHSCGYIYEILDDLLEVGIDAFQLDQPTLMGIERLAEKIGGKATLFS
                     PVDIQAILPTGNRQLIRDAATQMVDLFYKNGGLIAKDYGDYNTLRIKDEWAGWARERF
                     FEIGGFDPGKK"
     gene            64770..66152
                     /gene="mltG"
                     /locus_tag="PLF14_02135"
     CDS             64770..66152
                     /gene="mltG"
                     /locus_tag="PLF14_02135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOT70130.1"
                     /translation="MTDTDNDKIETEENTELPLEQVDDTANEQDFDQGREQGIEKKQS
                     KILTFIDGIWKFFTKEQAEKRRERKKNKKIRIVVYKPKSQFRKFISLILWLGFLFTLG
                     FVFYKGYTFTNTLVKEVNVFDAKAAIGTIEEKNRKVVNIPKGANTKEIAKILVNEGII
                     INDTYFWVYSLLMGNDGNYKSGNHIFDASVTYDSGSIDVVTAQGYEMLIYILSSDSVP
                     NPSKKIFFTEGLTMAQTVEKFTENDIRTKEEFYEVCNNYEFEYDFVKQIPKDSRRTNR
                     LEGYLFPDTYIFDVTVNAEEDIKKMLDNFNSKFNETYRAKAQALGYTIDEVVIIASLL
                     EKEASTMDERAMIAGVLYNRMNSKETYLNYLQVDATIQYIYLNETGKLKEQLTNEDHE
                     IMNSYNTYMYKGLPPGPICNPSLASIAAALYPETHNYFYYVAKNDGTDAHAYATTLEQ
                     HNYYVKLYQK"
     gene            66155..66808
                     /locus_tag="PLF14_02140"
     CDS             66155..66808
                     /locus_tag="PLF14_02140"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HOT70131.1"
                     /translation="MEKRFNIVNNDVEKYIQDTLAPLRDNLQVMYRNALKNCVPVVRL
                     EVLRVLETYLILKKPYSLLELGTATGFTSIFFSEYLKDNGYIDTVENDLEMVKRAREN
                     IKEMAPDKNINVIYAEACDYLKNINKKYDIIFIDAAKGHYEEYFDICSNLVNDNGIIF
                     SDNVLYRGMVAKGDKIPHRQKTLVERLHRYLDKITKDERFCTSILSVGDGLSISVKK"
     gene            66754..68052
                     /locus_tag="PLF14_02145"
     CDS             66754..68052
                     /locus_tag="PLF14_02145"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOT70132.1"
                     /translation="MHIDIIRRRRIIDFGKEMRAIELLAPAGNMEKLRIAYAYGADAV
                     YLGLNKFSLRDKADNFSYEELEHAVAYARALGKRIYVTVNILPKNKDLEEIKKTFDRL
                     SDILPDGIIIADIGLIELAKKHAKNIDIHISTQANILNKESAKAFCRLGAKRLILGRE
                     LSLSDVAQIKDAVGAEHEIEIFIHGAMCISYSGRCLLSSYMIGRDANQGDCAQPCRWK
                     YALMEEKRPGQYFPIYEDDLGSYILNSDDLNMINHIPELLECKADSFKIEGRMKSAFY
                     VATVTKAYRTAFDDCIRDKQKYLSSLEEYNEIVSMASHRPFTTAYNLGYEDFVNKTRE
                     SMTYSNSNYIREYTYCGIVEDYNKELGLARVHELNPVYIDDTVILMSPHINTFEQKVI
                     KLYDEDMQPIDKAHHPAMKYYLKTDIPVEKDTIIIKRGGQ"
     gene            68049..68816
                     /gene="proC"
                     /locus_tag="PLF14_02150"
                     /pseudo
     CDS             68049..68816
                     /gene="proC"
                     /locus_tag="PLF14_02150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
     gene            68832..69305
                     /gene="rnhA"
                     /locus_tag="PLF14_02155"
     CDS             68832..69305
                     /gene="rnhA"
                     /locus_tag="PLF14_02155"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HOT70133.1"
                     /translation="MSGDKKEKINGKSVRIYTDGACLYNPGPGGYAAILLYGNKEKEI
                     SGYKEDTTNNEMELTAALEGLAQLKKSCDVTVYSDSAYLINAFNQGWIDSWKSNNWTR
                     GKNEEIKNLELWQSLYEMNQKHNIKWVKVKGHSDNEYNNRCDRLANKAIKDNQNK"
     gene            69302..71185
                     /locus_tag="PLF14_02160"
     CDS             69302..71185
                     /locus_tag="PLF14_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOT70134.1"
                     /translation="MSKKYLEWNNSPEAIYLYSLLKTVFERILPRKRKTPSKKQLIKM
                     SSDLSYSSKQRLTKNNAEMYLKRNRNILNTALPHRNKLTISDNCFDEIYKKMAICELY
                     LFNRYNKENGHIPDMFTLFEDTGNVFQLKNNTVLSIYMERYLKKMAKEQILKLIPQNP
                     KDEFCETRKMKRYFVIHSGLTNTGKTYHALNALKEGKSGAYLSPLRLLALEVQERLMQ
                     DGVLCSMLTGEEEDIVEGAKHISSTVEKADYSQNYDVAVIDECQLIQDEARGCYWTKA
                     ILGIKAQTIHLCCAPEAEQILVKIIEDCEEEYDIIRHKRNTELIFSEQPFSLKNTQKG
                     DALVVFSRKSVLRVADELSRYNKKASIIYGALPYQTRKKQLGLFLEGKTDVVVATDAI
                     GMGLNLPIQRIVFLETEKFDGTVIRPLVPWEVKQIAGRAGRRGIYEKGYVSTVSDTNL
                     VKHNLTVPNATVEYAYLGYSDILLDIECDITQALRVWKSIPTGGFYVKQDISRTLNLC
                     DQIRKIYKDFSKRELLKLASIPFDEENRQVMELFKEYTLLFSYSQPIFKPVIISYTLD
                     ALEDYYKCLDLYYSFSRSMGYTPDLEWLKETKHIIAEKINKELLLNSSGQHAKKQRKK
                     SNY"
     gene            71214..71681
                     /locus_tag="PLF14_02165"
     CDS             71214..71681
                     /locus_tag="PLF14_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70135.1"
                     /translation="MKETSKQESDNRVNKGIKVIYYVTLTISMLVGLWHFFVPNMFKW
                     YDYLPMQYENLIVGIDYTNLCFSALLFGASLVLIILAKSAFKLNFETIVFYTFLTVVW
                     VFRACLAVFIEPWPLEPVPAAAIAQLISSVMLAVFMVIVTVVFWKNRKVHKKG"
     gene            71907..72158
                     /locus_tag="PLF14_02170"
     CDS             71907..72158
                     /locus_tag="PLF14_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002665791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70136.1"
                     /translation="MKMYKSKTWQFEVSGIDGNVILFGKNIFEYEWNNTGESVLVEDP
                     IYHQEFKFPVYTVNIDGQSHSFVAGEFSNCIWGFYLFKY"
     gene            72216..72425
                     /locus_tag="PLF14_02175"
     CDS             72216..72425
                     /locus_tag="PLF14_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70137.1"
                     /translation="MLHVEKLDFTGFYECKDSQKQRFVYDFFSGEYVIIMLKGENVVV
                     NRVSELPQSKKFEKLKELDRKNIKN"
     gene            72534..72752
                     /locus_tag="PLF14_02180"
                     /pseudo
     CDS             72534..72752
                     /locus_tag="PLF14_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018107267.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein Fic"
     gene            72788..73399
                     /locus_tag="PLF14_02185"
     CDS             72788..73399
                     /locus_tag="PLF14_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70138.1"
                     /translation="MTTDDKMLVHCVWEHNKNDSLIYSSNVIGAFTRGASKEEALGKM
                     EREIESYFLWTGETPPSSIEMIIIQESVTNLSISDADSEVLFETEKMDLSIEEYERLK
                     ALVLKSAKDFLSLYNSFPDKNQSVLPIRKTFYGTAPRTASEMYVHTKNVNEYYWGEIG
                     LDVSNDGTIVENRIRGFEELEARGNFLSGKVYKGSYGEEWSIP"
     gene            73889..75223
                     /locus_tag="PLF14_02190"
     CDS             73889..75223
                     /locus_tag="PLF14_02190"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HOT70139.1"
                     /translation="MNLGKETETLEFKKSTSEIKAAMISIASILNKHGIGTLYFGVKP
                     NGDVVGQNVSESSLRDVSRAVYESIKPQIYPAIDEIVIDGKQLIKVEFSGDSKPYSAA
                     GRYYLRTADEDREVSPEELKTFFEANKYRTKWENSKSDTLSKQVDKLAVKSFWQKAIN
                     AGRLPEGRYTTPLILKRFGLVKDEHLTNAGEVLFGNTRPVSLKVGIFASDEKLTILDM
                     RLYEDNIYNLLKKAEDYIFKNIRWKVEIIESERSEIPEIPTAVIREVLANSFAHAMYG
                     SRTNHEICIHPSMITIYSPGDYASRYKPEEYIKGNVESEIRNATISKILYLSKYIEQF
                     GSGFKRIDSLCKDSGIKYSYESKDNGFKFLIYRPQVRSDILDVTLDVTLNGTEKAVYA
                     ILKQKPDSSREEIADKISKTVRTVQRALNSLVEKGYIRRVGSKQNPVWEVLK"
     gene            75262..75675
                     /locus_tag="PLF14_02195"
     CDS             75262..75675
                     /locus_tag="PLF14_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HOT70140.1"
                     /translation="MTFCGDDCCRECSKLSECGGCEKCNGHPFGGSCIAERNSNFQEL
                     KSRLIVEINHLKIDGLIVKDLFLLNGGYVNLEYPLTNGTTVKFLNKNDVYLGNQIEKE
                     KSERCYGVVANENFILICEYGCNGSEPEIILYKRR"
     gene            76096..76341
                     /gene="secG"
                     /locus_tag="PLF14_02200"
     CDS             76096..76341
                     /gene="secG"
                     /locus_tag="PLF14_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOT70141.1"
                     /translation="MSEALKIILGIVHIIVCLATIIVVLMQSGKSEGLSGAITGGAET
                     FFGKGKGRAIDKLLSKYTVYIAIFFAVTSIFLYLVLK"
     gene            76523..77602
                     /locus_tag="PLF14_02205"
     CDS             76523..77602
                     /locus_tag="PLF14_02205"
                     /inference="COORDINATES: protein motif:HMM:NF024151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70142.1"
                     /translation="MLIRLIGLLVAFGLFIFLICQKKVLKWKKAILVIVSFVLVVFMI
                     IQVFFPFIPAKKPTGPYGVITETAFYKYNTDIPGMATNGNEREIPVKVWFPKDAQAKS
                     HPLFLFSPGSFGVAESNETLFLELASRGYVVVSMNHPYHSFSCKMSDGRNINMDGKFI
                     QSVMNSQGSEDLPGTLSSFKEWLKVRIDDINTVLDCISDFQNDYDYERYIDKERTVLS
                     GHSLGGSAVLAVGRERAQEIKALAVLEAPFAADIVGIDKDRYIFTDQPYPLPVLHVYS
                     DALYGKMDEITTYDMNTRLMKSDNPMYVNKHIKGVGHLGLTDMRLVTPVITYLMDGGL
                     DTRPAAETLSELNEYVLDFLNIYNN"
     gene            77715..78221
                     /gene="ruvC"
                     /locus_tag="PLF14_02210"
     CDS             77715..78221
                     /gene="ruvC"
                     /locus_tag="PLF14_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOT70143.1"
                     /translation="MRILGIDPGFAIMGYGVIDYFKNKIEVLEYGVIKTQAKTPFDLR
                     LLQNYRGINEVIEKYKPEHVAMEQLFFNTNQKTAINVCQARGVAVLAVAENRLEFFEY
                     TPLQVKQALCGYGRADKNQIQQMVKVLANLKDIPKPDDAADALAVAICHANSYREERK
                     TENKCMSI"
     gene            78206..78790
                     /gene="ruvA"
                     /locus_tag="PLF14_02215"
     CDS             78206..78790
                     /gene="ruvA"
                     /locus_tag="PLF14_02215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOT70144.1"
                     /translation="MYEYIKGEVVAKKHDYVVVDNAGVGYRIFTSINTMSKLQTHEQT
                     LLYTYLHVREDVMVLYGFACEEELFLFNMLISISGIGPKAALSILSVLSYQEVATAVV
                     SEDAASLSKAPGVGKKTAMRIILELKDKLKGLYAAEGDAVKQIAVSDKYSEAVNALLV
                     LGYNTKYAREALNKVYDESDSLQDIIKKALAEQI"
     gene            78803..79804
                     /gene="ruvB"
                     /locus_tag="PLF14_02220"
     CDS             78803..79804
                     /gene="ruvB"
                     /locus_tag="PLF14_02220"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007790131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOT70145.1"
                     /translation="MEEERFVTPSFIKNDQSEASLRPKVFDEYFGQEKIKENLKVFIE
                     AAKLRREPIDHVLLAGPPGLGKTTLAGIIASEMGANLRVTSGPAIEKAGDLAAILTNL
                     DENDILFIDEIHRLNRTVEEILYPAMEDFALDIIIGKGPSAKTIRLDLNKFTLIGATT
                     KTGMLSSPLRDRFGVSCRLEMYTVEELSKIVKRSAGILGFNINEYGANEIASRSRGTP
                     RIANRLLRRIRDFAQVEGEDVITSEIADYGLQKLEIDKIGLDNIDRAILETIITKFNG
                     GPVGLDTLAASVNEEKDTIEDVYEPYLLQLGFIKKTPKGRCVTPHAYKHLKIPYEPK"
     gene            79791..80327
                     /locus_tag="PLF14_02225"
     CDS             79791..80327
                     /locus_tag="PLF14_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HOT70146.1"
                     /translation="MNRNKILLHTCCAPCLVYVNEKLNEMNVDFDSYFYNPNIHPKKE
                     HERRLLTLRSYTSRFNIPLIEDDEFLQYLWENGFQCKDCYNIRLEKTARYAKNNGYKG
                     FSTTLLVSIYQNHQLIIDIAESVADKYGIEFFYHDFREGYRIGQNKAKEYGLYRQKYC
                     GCINSYNSSKYKDKIKWD"
     gene            80345..81556
                     /locus_tag="PLF14_02230"
     CDS             80345..81556
                     /locus_tag="PLF14_02230"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOT70147.1"
                     /translation="MRLFNKKKIYLFDGSKGFMLQKYGLQKGELAESYNITHPDTVMR
                     IHKEYCDAGSDIIQTNTLCSNRLVLKRHGLEGLHDKINSQGIELAKQAIDGRNILIAA
                     SIGPIGELLQPFGPLTFEYAYSLYKDQIYACRNADVINFETFTDLKEMRIAYLANRET
                     VNLPVICNMTFEKNNMTLMGHSPKVCAGILKKMGADVVGVNCSNGPDKMGDILKDIAV
                     FEDFVCVKPNAGVPSFVDGIAHYDQGEIEFCGYSDNLLDFNVGIIGGCCGTTPEYIKA
                     MNYIKDKDRSITVPVDKSRYIFCQYGYVDIDDSYETVDFDIIDGDIYDNIDNAYDINE
                     EPCDVYNVFYRGDNAQFMFELINQLQDILKKPFIFHIDDKVCLDSALRAYCGIAGITG
                     CDFSKYGAVKI"
     gene            complement(81564..83414)
                     /locus_tag="PLF14_02235"
     CDS             complement(81564..83414)
                     /locus_tag="PLF14_02235"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT70148.1"
                     /translation="MKNRFSDIPFFIRIVLLVLAILVISLIITTVIYTVASEEIFVTN
                     QLERLKEETDMLASLIPGDWVTRDQLENFFDKALQAESSILRSFIFVSDKLNVEVYSY
                     APAIRRETQTVAKADMDKFYRNNLSKHMRGVLAGQYISLKNTDNEFNSDMFIIMRPIF
                     SQYGDVQAVLTVCKFIDDYSGELRTINNALVLTMIGVSLLMLFPIYRMSKSITKPLLE
                     IRDVAVAFGKGDFSVKADESYPAELGQLSLTMNHLSSQLGKTITSLTRETNILQGVLN
                     SMNQGLLVVDESLSPMIINPALPKFFKSTMNMNEKHSIIPIQAVWEDFEQCLSEQQYI
                     ENIYEFQGNMILCVINPLKVNDALIGAMGIFSDITKEIRLEQTRRDYVANVSHELKTP
                     LTGVMGLLAPLKDGIVTDPDKITKYYAIMSNELQRLNRLINDLLMLSRLQSASEAFAM
                     DKVNLSAIIVDKIESYHFTYEDNNLTIDFTGPSQPVLVYGNADRIEQILTILIDNAIK
                     FSQNSADKKINNEKIKIKIAIVKKDDKYIVSVQDYGTGISKEDIEYVFERFYKSDKSR
                     SSAGSGLGLAIARETLKRMNEDIWVESDGKSGSVFSFTLSEYKPNIDKVK"
     gene            complement(83404..84093)
                     /locus_tag="PLF14_02240"
     CDS             complement(83404..84093)
                     /locus_tag="PLF14_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012993453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT70149.1"
                     /translation="MNIHVLICDDDPLVHEALSVYFDKFGMTHQSAFNGQECLDLYQK
                     GTFDVIVLDIMMPVIDGMEVCRRIRISSTIPIILLTAKSEEVDRLLGFDLGADDYITK
                     PFSSLEVIARIKAIINRVRNPSQKESSGKVSFKQLTVDITRYSILIDDVPTHATPKEV
                     EVLFLLLSNPKIVFTREQIMNLIWGYEYTGDTRTIDTHIKRIRAKLPAEIQKCIQTIY
                     GVGYKFECNEE"
     gene            84335..85588
                     /locus_tag="PLF14_02245"
     CDS             84335..85588
                     /locus_tag="PLF14_02245"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HOT70150.1"
                     /translation="MIYDFDKVIDRHNTNSLKYDFYEDSGYTVDHIPLWVADMDFPVI
                     EEIKSAISRINAVGIYGYTGISDSYYDIVAEWMYRYHNFKVEKDWFCITPGVVYALSV
                     AVCAYTQPGESVIVQTPVYSPFYGVINNNKRKLVRNPLVYKNGSYYVDYVDFENKIVE
                     NDVKLFIMCNPHNPVGKVFTKEELIRMCHICCKHGVIIVSDEIHSDFVYAPHKHTVLA
                     NLNSNFEQSCVICTSPGKTFNITGLKASNIFIPNPELKKLYDEEMAKRGPGLVNTLGI
                     AACVAAYRYGRPWLNKLLKYLQGNIEYVTDFINLNLPKIKVVQPQGTFLVWIDCMEMG
                     LENSKLNNFFKDEAKIWLSPGDKYGPEGEGFQRINIATSRSILEKAMNRIKTAYDVLF
                     YERKANSDDKETDNQSAENLDNEVQ"
     gene            85625..86890
                     /gene="serS"
                     /locus_tag="PLF14_02250"
     CDS             85625..86890
                     /gene="serS"
                     /locus_tag="PLF14_02250"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOT70151.1"
                     /translation="MLDINRIRNNREEVEKALLKKNFKIDFTELLDFDAQRRSKIFEV
                     EQMKAKRNTVSGEIPKLKKQGTDVTDLLNEMKKISLSIAELEKEITSIEAQIKDIMDY
                     IPNTPADDVVAGDATQNQPVRYHGRKPEFDFTIKDHVALCEALGIIDYKRGAKLSGSG
                     HWMYRGDGALLEWALLNYFITEHIKDGYEFILPPHILNYECGYTAGQFPKFGDDVFKL
                     TENDQFLLPTAETALINLHRNEVLREEELPKKYFAYTPCYRKEAGSYRAEERGMIRGH
                     QFNKVEMFQYTVPEKSEEALMELVAKAEALMNGLGLHFRLSKLAAADCSFAMGKTYDV
                     EVWIPSMGIYKEVSSASNAYDFQAIRGGMRFKRTATGKNEYLHTLNASGLATSRVFPA
                     IIEQNQHKDGSVTVPEVLRKWVGKDKLTV"
     gene            86887..88131
                     /gene="lysA"
                     /locus_tag="PLF14_02255"
     CDS             86887..88131
                     /gene="lysA"
                     /locus_tag="PLF14_02255"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOT70152.1"
                     /translation="MIEYFSEKTDFYKQHKPSELLSAYGSPLYVYNEDILRERARQIK
                     NFVNMPNFTVSYSVKANCNLHILKILKEEGLHADAMSEGEIFVTEQAGYEKDRIFFVC
                     NNVSAEEMKYAVDRGIMVSVDSLSQLELYGKNFPKTKVAVRINPGIGAGHHKKVVTAG
                     EATKFGINIDKANDVIRIAEKYNLKIAGLNQHIGSFIMTSTEYIKSVKELLQLSSLFE
                     DIEFLDFGGGFGIPYNKQQGQQPLDLKQFGKELKDVLTSWQKEKKKNILFKCEPGRFV
                     VAESSILLGKVYAKKHNYNKIYIGTDIGFSVLIRHAMYGAHHDIEIYRNDKPVKNGAY
                     EFVNIAGNICESGDILAEERQLPVILEDDIIGVMDAGAYGYSMSSNYNNRLRPAEVLI
                     KNDGGLQVIRKRETLEDLIKGF"
     gene            88199..89503
                     /locus_tag="PLF14_02260"
     CDS             88199..89503
                     /locus_tag="PLF14_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOT70153.1"
                     /translation="MNTMIKNADILTMDEKFTILKNQVICIEEDKIVYIGEDEPFFFI
                     PNRMINAKNKIVMPGLFNAHTHCAMTLMRNRSNDLPLERWLHEQIFPVEANLTYKDVY
                     NGAMLGIAEMIRGGTVAYLDMYYIHEASLEAIKETGIKANLSYGVSTSGKVKELGKQG
                     AEQYCKNFILTNKNAFKGRLNTSVEIHSVYLVDEPELILSAKMAKETDTLIHIHLHET
                     KAEVENCIKTYGMTPLQLCAKTGVLDNRVTAAHTVHVNDEDMLLMKEKNVIPVHNPSS
                     NLLLGSGIADIPKMLKAGIKPAIGTDGAASNNTLDMFKEMHLTSLIHRGYKGDSNVIN
                     ARQVLTMATANGARALGFDKCGMIKTGMKADIILVDTESLNMTPFHDPVSALVYSVRA
                     TDVDTVMVNGEILMKDKQLLTIDEEKVKYEAAGSAARLYNLF"
     gene            89575..90834
                     /locus_tag="PLF14_02265"
     CDS             89575..90834
                     /locus_tag="PLF14_02265"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006352856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HOT70154.1"
                     /translation="MYSTEKAYKQAKEYYKRFGIDTEKAMKLCDKVPISIHCWQGDDV
                     GGFEKSASDLTGGLQVTGNYPGKPRTADELRSDLDVVMKYLPGVKKINLHAIYQEAEE
                     FVDRDAIKPEHFKKWADWAVDRNIGLDFNPTYFSHPLSENGTLSAANDEVREFWIRHG
                     IACRRIGQYFYERTGKPCVVNHWTSDGSKEIPHDTLSPRLRLKDSYDRIFAETADVKG
                     VIDGIESKLFGIGLESYTAGSFEFCLSYVINKRKNHIILTMDTGHYHPTEVVSAKLGA
                     IFAFQKSILLHVSRPVRWDSDHVVSFDDETRAIFEELVKLGKLNNTYVATDYFDASIN
                     RVMAWVVGLRNTRKAILAALLQPNEMLSKVATRNDNTGVLAIREEFKSAPFGLVWDYY
                     CEKAGAKSGIEWINDIKQYEQDVLLKR"
     gene            complement(90977..94012)
                     /locus_tag="PLF14_02270"
     CDS             complement(90977..94012)
                     /locus_tag="PLF14_02270"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOT70155.1"
                     /translation="MKKYHKDISILGKNVLAPRATSIPYSDVADAKKDIRGLSPYFKL
                     LNGKWNFKYCENEYDVDESFYLPEYDDEDWDIIPVPSSWQVLGYDKPQYINVRYPYPV
                     DPPHIPDINPVGLYRTIFVLPDSFAARNVIIHFGGVNSSFILYINGQEVGYSQGSHMP
                     SEFDITKYVRQGANLIAVQVYKMSATSYLEDQDFYRLSGIFREVYLYSTHNNSIMDFT
                     VKTTLNDIFVSILSSETKGITYVYELYDKDDVKIFGKSVKKPFIDMHVEGVLPWTSET
                     PNLYTLILQLKDAKGTVTDIRSCKVGFRTVSINDGVFMINNTPVKLKGTNRHDTYALT
                     GHAISREVMESDIVMMKRHNINTIRTSHYPNDPYLYSLCDLYGIYVIDEADIETHGFY
                     YNDPEYDLSDKPEWTHHFVSRAARMVQRDKNHPCIIMWSLGNECRFGNNHKAMINYIR
                     SVDNRPVHYERALTDQSVDVVSAMYTSHEKLEEIGAASDTRPFFLCEYAHGMGCGPGG
                     LKDYWDIIYKYPNLMGGCVWEWADHGLLAYTEDMVPFISYGGDFKDFPNDGNFCLDGY
                     NTADRIPKAALIELRKVYEPVKVINFDKHTNTVTFKNMLDFADISNIGVYYELSKDGN
                     ILKVGNIPGLNIAPQETKTYTLEFDANIFGDVQLTFFFEYNESTPFAEKGYEVTRSQI
                     IIDEKFEYKKPLERNDLAWVETRTEYIVLGESFKVSFSKMFGNLYSYYFNGNEMLLGT
                     GTSESFYRAPGDNDVKIAKKWEEYGLDRLQKRIESYSFKANTKKAVFKVVSVFAATTK
                     KPVLKTVTEYTVYTNGQITVNSGYEPLNDFIKFQNTDGNRECLYLPKFGLTMQIPFEY
                     EYVKWFGRGFHQSYEDMNSSAVVGIYEGLIGDLQEHYEKPQENGNRMDTRWASFKNVQ
                     GQGVFFGGLDTFNFSASYHDAYQKAKARHLHELEESEGITVNIDYKQSGLGTASCGPG
                     PEDKYLLYTNPNSFSFTLSPFSDSVLDEQEMFRLRHVKK"
     gene            94208..>94518
                     /locus_tag="PLF14_02275"
     CDS             94208..>94518
                     /locus_tag="PLF14_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70156.1"
                     /translation="MSNSKKKKTDKEKKALTAITVITVVVGIPLFVLFLSRYFTKNTG
                     ISTDTPVIQSPIATYQQTEIPAETVAPTQTVMPTETAAVQTPEPVPSSSINLNEIEIR
                     N"
BASE COUNT        31471 a        16135 c        21308 g        25604 t
ORIGIN      
        1 gaccgttagc cctgttattc tccaatgagt atctgtaagt ggtagtctca tccgattccg
       61 acaaaagtat atccataacg ggaacggaca aatccacaag atatatcttg tgctggttgc
      121 ataaaagata ttgctttcct tcagtatcgg gtattgcgat aagagggctg tttagtgttt
      181 cattctctct tccgttatat accggatatt cctgtccatt tacctgtatt aaatacaatt
      241 ttacttgata gccgtccgat gcttctaatg tgattgaacg ggtgtcatat atagccggca
      301 ggacctgaac accgtgctga tactgcataa gcatgtccag ttgtacaaaa tccgtctgtt
      361 ccgcaacttc agattgggta ggcttagcgg tagcggaagg taagacggtt gttgtagggt
      421 agggtgacgg actgttttta ttgcaagcgg aaagctgcaa tactatacat aaagttatca
      481 atgtcggcaa tatataccct ttattttttt tcttatgtat cttaaccatg tgtatatttt
      541 atccgtaata gtgttaaata acaataataa ttgttgtttg acagtataca gcaatggtat
      601 aatctatgtg tcacaaaaga taaggagggc aatgccttga aaaaaagaac agtatcaata
      661 tttgttttaa tatctcttct gcttttaatg cttccggtac aggcttcaaa agattcctat
      721 agtaagcatg aagtttccgc tatatattca aaagcttcgt tgaacgattt acaggcgaca
      781 agcgctattt tgctggaagt caataccgga aagattcttt actcgaaaga ggaaaataaa
      841 cagataccca ttgccagtct tacaaagatt atgtcggttt atcttgttat gcaggccatt
      901 gcaaacaagg agatatccta cgatacggtt gttaaagcct ccgctaaggc acacggagtg
      961 gaaggttctt cggtctatct caatctgggt gaagaattca ctttagagga aatgctgtac
     1021 gctattgaag tacgatcggc taatgatgct accgttgcgg ttgccgaggc tgttgccggc
     1081 tctcagggtg actttgtaaa cagaatgaat caaaaggccg tagaactggg tatgatggat
     1141 acctattaca gagactgcac gggattaacc gacgtggggc attacagtac cgttaaggat
     1201 ttgtctgtca tttgcagagc gcttgtaacg gaatatcccg atatattcag gttcagtacc
     1261 acactggaaa gggattttcg cgacaaagat cacagagatt atcaatatat ggttaacagg
     1321 aataaccttt tgaattacta tgacggagcg acaggattga aaaccggctt taccactgct
     1381 gcgggatact gtcttgtggg tactgccgag aagggggaac tcagtcttat tgccattgtt
     1441 acgggagagc cggacaataa ccacagggta gcggaaacgg ctttcctttt ggactacgga
     1501 tttgccaatt ttatgtttac cgatacccga gacgatatgc agacggtagg gtccattgaa
     1561 gtcagaaaag gcattgaaaa gaccgtggag acctatgcta agggaacttt gaggttttta
     1621 ttgaagaaaa ccgaaatacc cgagatatcc aaagagatag tatacaatgc ggctactttg
     1681 gaagcaccgg tggaaaaaga taccgaaata ggtaaggtgg tatactccct taacggcgaa
     1741 gtgttgggcg aaataccggt atacactgca gaggatatgg aaaaagcggg tttctggaca
     1801 ttgttgtggc gctctatcct ttcctggttc ggagtggatt gggagaaata ggggatgcaa
     1861 tttatttttt gattggtaat aagttgtatg gagaacaaaa aaaagatagg aaaaatctta
     1921 gttattatta taacagttgt gcttgctgta ggtgcattgc tgtatatttt acgtggcact
     1981 cttttaaact ttatagctcc tttcaatgtt acgaccttta atagcgatat tgtagtaaaa
     2041 aggtcggacg gtaaagaacc tctaaacatg ccttataggt attccaaggc tatattagac
     2101 aacagacagc tgtataaaga agagattgtc ggtcttaaca tatctacaat taagtacgac
     2161 ttaagtgata cgggcataag tttacgtaac tatgaagata ttttgctaaa tgctgatagt
     2221 agcgaagtta aggctgctat agattcaata aagtactgca aaggtataac cgcattaagc
     2281 ggtataattt ccgataaaga gggtagtaag atagagcttt atgaaggttg caccgctgat
     2341 ttgttattaa aggattataa gcattatgct attattccct cgactttcgg aaaacatatt
     2401 aacaaagaac ttgcggataa agataagatg ttttatctat taaatcctga caaaaacggt
     2461 ttagcatatt tcactataat aggagagtat gaagtaaagc aggacagaaa atcaaataga
     2521 gagcctgata ctatatattt atcttatgcg ggattaagta atgtagttcg gggagaacag
     2581 gaagatatat ctgcccatat tgattgcatg gagattgatg taaatgagca ggcggacctt
     2641 gtaaagttct catattttct ttccgaatat tttgcagata taaatgtaat ttctcaatac
     2701 gcaaaaagga taaacagatt taatgaagtt tattcatata tgtttgtaaa tacggtgggt
     2761 atagagccta tcattcttga agaggatacc gattttgaaa agacggtaat aactatttcc
     2821 cgtattgacg ggaaagaaaa ccttgaaatg tcacatatat atggtgatgc attgattaat
     2881 gagtatgacc aatattcaca gtatataacc gacattattc tcagtaccgg tagaaaaggc
     2941 attaacataa acgactatcc tccgtaccgg gctccaagat acggtttgaa tttcggtggc
     3001 actactcatt ttttcagtga tagtttagaa tattgcgaag aacagaaccg ccaattccca
     3061 tactaccacc aagcaattac cgggttaagg gaaataaagg gtatgaaaga aggcaccgag
     3121 ataacgtttt atactaatta tactgataaa gatttggtaa aagtaaggga agaagacata
     3181 aaatctatgt ctcgtcggaa taatgtttac gtaaaaggat atgctatatt acctgtgaca
     3241 ctacatgaat ctactcgcaa ttatcgcagt atagattatc atgtgttgtt cctttcggaa
     3301 gatagaatgt ttagagaaaa gcccgatgaa ttattgtata tgggtttcaa agtaataggt
     3361 tattatgaat tgccggaaga ctttgtaccg gaagagtata aatataggcg ggaggattat
     3421 gaaaagcaag gaaaaggaat cgtaagttca ctggataagt acggtaatga agttaaaata
     3481 actgattatg aacctgacgg gtatgacact atatatttta catatgcagg gtataatgat
     3541 aggtataagc tgaaacagtt taaaaacgaa tacatagagg ctataacaat agagacaaaa
     3601 agtgataccg atattacgca actggttaaa tacttggagc gatattttgt accatccgat
     3661 aatactgcaa aatattcaaa ggagaaaaat gtattgggtt tggagtatga gtactgttac
     3721 acgataacaa ataaagcaaa ttagcgaaca gtgcaaagcc gatctatatg ttcatcaagc
     3781 aaagattcta cttaatttcc aatattcaaa aacgtaatct gtttccaagc gatagttaaa
     3841 acaggttaag tataggcagt ttaagctgtc taagcataat atcaaaatag ggatatatct
     3901 ttacatatta aatcttaaaa tgtataatcg gtaaaaggaa aaggtgttaa ttatatgtgg
     3961 caaggccttt taaacacgtt aataaccatg ttgtatatga ttccggtaat gcttatatcg
     4021 cttacccttc atgagtgtgc gcacggcctt atggcgttaa aattgggaga taccaccgca
     4081 agagatgccg gtcgtttaac attgaatcct ttgaaacact tagaccctat gggtgcgata
     4141 atgatggtaa ttgccagagt ggggtgggca aaacccgttc cggtcaatcc catgaatttc
     4201 aaagacccta aaaacggcat ggcgttagtg ggacttgccg gacctctcac aaatctgctg
     4261 atatctttcg gagcggcttt tccggttgct ttgattgaac aggcggtgaa tttgcacggg
     4321 gtggaattta ccgttttttt aagaattaca tatactttct tttatttgct ttttcaggca
     4381 aatgtttttc ttgcactgtt taacctcata ccggttccgc cgtttgacgg atcgaggata
     4441 ttgttcggta tactgcccga caaatactat ttcagtgtaa tgagatacga aagaattgtg
     4501 ggaatgatat ttttagttgt tttctttata ttccggtcac agtttgcgaa tgtgttggat
     4561 ttaatcgctt cacccataac aaaagcgttt ttgtttgtgg caaattggtt tgtaggacta
     4621 ttttactagg aggtttttat gaaaaagaag gttgtattaa gcggaacaag aagtacgggc
     4681 tcactgcatt tgggaaacta tttcggagcg gtggaaaact ggctcaattt gcagaataaa
     4741 tatgactgct acttttttat agctgactgg cattcgctga ctaccggtta tgaagatacc
     4801 gataactata ttgccaatat taaggaagtt gttatggact tatacgcatc gggactggat
     4861 tccgataaat gtaacatatt tttacaatcg aaaaaccagg accatgcaga actgcatctg
     4921 cttttatcca tgattacacc gttatcctgg ctggagcggt gtcctacata caaagatcag
     4981 ttgagtcagc ttgccgagaa ggatataagg acgtacggct ttttaggtta tccctgtctt
     5041 atggctgcgg atattctcat ttacagggcg gagggtgtac cggtaggtga agaccagctt
     5101 gcacatctgg aaataaccag agaaatagca cgaagattca actatatgta cggtgaggta
     5161 ttccccgaac ctaaagctct gcttacaaaa gcaaaggtgc tgccggggct ggacggaaga
     5221 aaaatgagta aaagttatga taataccata ctgcttaaag aggaagatga tgtattgaga
     5281 gctaaaatca tgagcatggt taccgatccc gcacgtataa gaaagaccga tcccggacat
     5341 ccggaaatat gttcggtata tgcctttcat aaggtcttca atcaggcgga agttgcttcc
     5401 atctgtgagt cctgtaaaaa aggcgagata ggatgtgttg cctgcaagaa gaacttatac
     5461 ggaaaggtat cggagttctt acgcccgata caggaaagaa gaagagagat tgcggggaaa
     5521 cccaagctta tgaaagaaat attggaaaag ggcaatgcca acgctaaaat aaagacggat
     5581 gaaactatga ctatggtaag aaaggccatg aaggtaaatt gggagtagga tatcagttaa
     5641 aactggaaaa ctttgaagga ccgctggatc ttctgttaca tcttatcgat gagaataaga
     5701 tgagtattta cgacataaaa ataagtataa taaccgaaca gtatctggat tatcttatgg
     5761 ctatgcaaca gctggatatg gatatagcaa gcgattttct ggttattgcc gccacattgt
     5821 tgcatataaa atccagatcc atgcttccta aggcaaaaga agatgaggtt gacagcgaga
     5881 ccgatttgat aagacagctt atcgagtata agaagataaa gaaagcggga gagcggttca
     5941 gagaattaca tgaagaaggg cagaagagtt tgcattcctt tgcctccacg gaagacttcg
     6001 gagaatcgga aaaaacctat acactttgca aaagcatgct ggtaatgatt tatcgggaaa
     6061 ccatgcaaag gcagattgat aaaagaaatc tgaaagcgga gaatataaaa gagattgtaa
     6121 aaaaagagaa aatatcgctc ataaaatcca tcaggaagat aatcgatgtg ctctttaagg
     6181 aaaagaggat aagttttttt acccgattta aagataactc ggtatccaat gcgcaactga
     6241 taacggattt tctctccatt ttagttttgt caactcagaa aaaagccaca cttaaacaaa
     6301 aaagaccttt tgaagatata atcgtggaag gaaccgaaac attaagctat ccggataata
     6361 tacaggaaat atacgattgg gagtaagata tggataaaga gtacattata aaccagataa
     6421 ccgcaatgct ttatgctaac ggtgagccgg tgcccttaac caaattggaa cagatactgg
     6481 agaccgataa aaaagagata aaaaagataa ttgatgagca tataacctat ttaaaagaca
     6541 ttaagtccgg cattctgata agacagatag aagactctta ccagatgtgc accaacccga
     6601 tatattttcc cgcagtccgt cagctgtttg agaagaagag gaccgataca ttgtccagag
     6661 cggcatatga gactctggca ataatcgcat ataacaaaga agctaccaga gcaaagatag
     6721 aaagtataag aggcaattcc gacagtccgt taaagacatt actggaaaga ggtctggtaa
     6781 tcgaagcggg aagactggaa gctccgggaa gaccggtgtt atacaagccc gcagaagagt
     6841 tctacagagc ctttgatgtg tcttctttgg atgatttgat tccgctggac agttttgatg
     6901 atgaagaggt gacggataca gaggaaaccg aagtaaccga agtatcggaa taagaaaatc
     6961 cgtggaaaaa atgcgtttac aaaaatatat tgctttatgc ggttatgcat ccagaagagc
     7021 ggcggaagaa cttatcttca acggctcggt atcggtaaac ggcaagattg tacgggatat
     7081 tgtaatggtg gacgattccg atgaagtaag ggttaacgga aaacttttaa agacagaaac
     7141 aaaaaaactg tatttcgtat tcaataaacc taatatggtt ataacctcca tgtcagataa
     7201 gttcggcagg aaaaccgttg cggacttttt taacaatatc gaggaaagag tttatcccgt
     7261 aggaagattg gattacgatt cctccggtct cctattcatg accaatgacg gtgactttgc
     7321 aaattatctg atgcatccta aaaatgaggt aacaaaaacc tatgccgttt cggcaaaggg
     7381 taatataacc gatgagataa aagaaatgtt ggaaaacggg gtggatatag ggggatatat
     7441 aacagggaaa gctgaaataa ataatatagt aaattatagt gacgggaaat gctctgcgga
     7501 aatatcgata aaagaaggta aaaaccgtca ggtaagaaaa atgttcgaag cagtcggcat
     7561 aaaggtatat gcactgcaaa gaatatccgt aggggctgtg caattgggag atcttaaaaa
     7621 gggcagatac aggtctctta cgcaaaaaga attgcgtgat ttgggatatg aaaggcagtt
     7681 ttagcccgag ttttccgtaa tatttctatt gttgtttttt ttgtacagca aaccggcaat
     7741 aaacttaata atttaccaaa attcgtttgt acagatctgt tataaatgat ataatcaact
     7801 gcaatggcat attatgaatt agaggtaaat aaatgagcat gacagaaaaa gtagatgaat
     7861 taagaaaaag aaagcagcaa acatctcttg gaaggggtca acagcggatt gaagacggta
     7921 aagccaaagg aaagaagatg gcagccgaca gaatatccaa gttgctggat tcgggttctt
     7981 tcatagagac cgatgctttt acccaatcaa gaagtacgga tttcgacatg gcctcaaaaa
     8041 gagcagacgg tgacggtgtg atatgcgggt acggtaagat taataaccga cccgttttta
     8101 ttgcttcaca ggactattcg gtcttacacg gctcggtagg cgagatgcat gcaaagaaga
     8161 tagaaagaac catcgattgc gctatcaaga cgggagtacc tgtcatttac ttgttggatt
     8221 ccgacggtgc cagagtagaa gaaggtaccg atgtgcttgc cggcatcggc ggagttttaa
     8281 caaaacagtc catggcaagc ggagtgattc ctcaaataag tgtagtactg ggcaactgcg
     8341 caggtatttc atcattgttt acggccatga gtgattttgt tgttatggcg gacaagataa
     8401 gctgcatgta tatgaacggc cccgaagcta tcttaggtat ttcgggagta aaaacaaccg
     8461 acgaagagat aggaagtgcc gctacacatg caacatcaag cggtaatgcg cacatacgtt
     8521 gcgagaatga agatgactgt ataaatgcgg ttaaactgct tttatcctat cttcccgata
     8581 acaatcttac cgaagcttta aaacttgaag tatcggatgc tcccgacaga actgcggaaa
     8641 atctgtatga gacggtagcc gattcatatg atatgagaga ggttatcact tcggtaacag
     8701 atacatcaac ctttatggaa atacaatcgg agttcgctgc ttccatgata acgggttttg
     8761 caagaatgaa cggtcaatcg gtaggtattg tggcaaacaa tcctgcggta aatgaaggtc
     8821 gtatcgatat gcatgccgcc gataaagctg caaggttcgt aagactttta gattgcttta
     8881 atattccgat aattactttt acacatacat cgggctatgt tgcagatgca ttgcaggaaa
     8941 aagaaggact gataaggcat gcggcaaaaa tggtatatgc attcacggaa gccgatgtgc
     9001 ctaagataaa tataatcacg ggtaaggcaa taggcggcgc ttacatagcg atgaacagca
     9061 aggctacagg ttgcgatatt gtatttgctt atcccaccgc aagtgttgcg gtaatggaca
     9121 gcgagggagc cgccaatata atttacaacg agcagatagc aaaaagcgac aatcccgttg
     9181 agttcagaaa agcaaagatt aaagaatatc atgagcgatt cgcatcacct tacgaagccg
     9241 caaaaagagg atatgtggat gatgtcataa atccggcatt tacgcgtgct tacgtaataa
     9301 ctgcattgga aatgctcaaa tcaaaaagag aaaatcgtcc taacagaaaa catggcaata
     9361 tgccgcttta ataaacggag gaaatatata tgaaaaagtt catagcaaga gttaacggaa
     9421 aagaatatgt agtggaattg gaagaagtta cgggaacacc tgccgtttcg gcacctgttg
     9481 ttaccgtagc gacgcctgtt gctgcacctg tagttaagga agaagtaaag caggaagttc
     9541 ctgctcccaa aaagaccgta tcttccggta cggcaggctc tgtcagaata gaggctccca
     9601 tgcccggtac tattttaagc gtaaaggtat cggtaggaca ggctgtcaag aaaggtcagg
     9661 ctgtagccat actggaggca atgaaaatgg agaacgatat agttgccgca caggacggtg
     9721 ttatcgtatc ggttaatgta tccaacggag agagcgtgga aacgggacag cttttagtgt
     9781 ccatggatta gaaagggttc aagatgagag tacaaatcac tgagacaatt ttgcgggatg
     9841 cgcatcagtc gttaattgcg acaaggatga gcactgaaga tatgaaagat gccttacagc
     9901 ttctggataa gatagggtat ttttcattgg agtgttgggg tggtgccact tttgattcct
     9961 gcgtcaggtt tttgaatgaa gatccgtggg aacggttgag atttataagg aagaatgtta
    10021 agaataccaa actgcagatg cttttaagag gacaaaacct tttgggctac aagcattatg
    10081 cggatgatgt tgtggaatat ttcgttcaga agtcggttgc aaacggtatt gatattataa
    10141 ggatattcga cgcattgaac gattcgagaa atctcgagac agccatgaga gcctgcaaaa
    10201 aagaaggcgg gcatgcacaa ggagcaatat gttatacaat atcgcctgtg catactttcg
    10261 agcagtattt aaaagatgcc aaatatcttg tggaagcggg tgccgattcc atatgtatta
    10321 aagatatggc gggactgttg cttccgtata cggcttatga tttggttaaa gtattaaagg
    10381 aacatgtcaa gcttcctatt caggtacata cccactatac cgccggaacg gcatccatga
    10441 catatattaa agcggccgaa gcgggagcgg atatcttaga ctgtgcttta tcgccctttt
    10501 cacagggtac cagtcagccg cctaccgaga gtattgttgc ggcactcaga ggtacgccat
    10561 atgacaccgg tctggtttta gcggatatta atgaaatatc acagatattc agagcggtac
    10621 gtgaaaaata tatcaaatca gggttgctca atcccaaggt tatgggtgtg gacatcaaca
    10681 ctctcatata tcaggttccg ggcggaatgc tttccaattt ggttaatcag atgaaagaag
    10741 cgggtaaaga agatcagctt caggatgtgc ttaaggaggt tccgagagta agagaagatt
    10801 tcggatatcc tccgcttgta acacctacaa gccagatagt gggaacacag tcggtaatga
    10861 acgtcatttc cggcgaaaga tacaagatgg ttcccaagga atcgaaagac cttgtagcgg
    10921 gcaaatacgg aaagactccc gtagcaatta aacaggaaat aatagataag atactgaaag
    10981 gtgaaaagcc cattactcac agaccggctg atgacattgc tccggctctg gaagaaacaa
    11041 ggcgcgagat gaaggaatat ttagaacagg aggaggatgt gcttacatac gccatgttcc
    11101 ccaaagtggc aaaggatttt ttcgaacaca gaagggcggt aaagtataaa ctggacaagg
    11161 atcttaccga catggaggac aaggtccatc ccgtataaaa tgaattgtaa acacctttag
    11221 gtttcaaaaa tacctgaagg tgttttttca ccccctgcgg gccgggaaaa tgcaggattg
    11281 gatatttaat tattcataat aagtggtata tataaagaaa acaggtgaaa cgatgagtat
    11341 taaagacaga ttgagaaaca caaaaggctt cagtaaagga cagaaaaaaa tagcggaata
    11401 tattttggcc aatttcgaca aggctccttt ccttacggca accaaactgg ctgccgaagc
    11461 cgatacctcg gagtccacgg tagtgcgatt tgccattgag ttgggttatg acggatttcc
    11521 cgatatgcaa agagatatga aggaagttgc ccgacagact ctcacttcgg cagagcgcct
    11581 tatcaggata tccgatacgg tggatatgga taatgtgctt atgtcggtaa tgaaatcgga
    11641 tattatgtcc ataagagaaa caatgaatat gatagatgtc gatgtattca ataccgttgt
    11701 ggataagctt tcagccgcaa agagaatata tattttgggt atccgaagct cctattccgt
    11761 tgcattgtac atgtccatat atttccgtat gatttttgac aatgtgcatc ttgtgggctt
    11821 accgggagcg gccgatgttt ttgaagagat tatatccgta aacaattccg atgcatttat
    11881 cggtataacc tttccgcgtt actccagaag gacggttcac gccatggaaa tagcaaaaaa
    11941 caacggagca tataccgttg ccttaaccga tagtatggat gctcccataa ccgaggtggc
    12001 acacaatgta cttgttgcaa gaagcggtct taccggattt gtcgattcgc ttaccgctcc
    12061 gttttccgtt gtcaatgctc tgatagccgc ctgcggcata aaaaaagata aggagttgtt
    12121 gaaaaagctt cagaatttgg aacaaatatg gaaagaacac tctatctata cgatggaaaa
    12181 caagaagaaa tgaaaaaaag ttatgatgtc atagttgtcg gtgccggtgc agccgggatg
    12241 atggccgcta taactgccgc agataaaaac ttcagtgtat tggtaataga gcaaaaaaaa
    12301 caggaaggca gcaagatact tgttaccggt aaaggccgat gcaacattac caatgatacc
    12361 gatagtgcag gtgtaatgaa aaatgtaatg tccaaccgca cttttcttta ttccgctctt
    12421 tttacttttc ctccgtcgga gattatccga tatttcaaca acataggtgt agccaccgtt
    12481 accgaaaggg gaagaagggt ttttccaaag tcgggtaaag cggctgatgt acgcgatgcg
    12541 cttgtaaaac agatgcgaaa gtcaaaagta aatgtactta aacgcaataa agttacaagt
    12601 gccataaaag gtaacgacgg tttttactat gtgaatgtat tgaatgatga taatatgacc
    12661 gattgcttca gatgtgtaaa cctcatactt gcaacgggag gaatgaccta tcggtccaca
    12721 ggaagcgacg gctccggtta cgagctggca aaaaaactgg gacatacggt taccgagctt
    12781 aaaggtgcat taacaggtct tttgacaaaa gaaaaatggg tagccgagac tatgggactt
    12841 acattgagga atgtgtcgct tacggtaaaa aaagacggta aggcattata caaagagcaa
    12901 ggggagttgc tgtttacgca tttcggtata tccggtccgg ttgtacttac agcaagcaga
    12961 tatcttttgg ataccgatta cagaaacacg gtagcggtta tcgatcttaa gcccgctttg
    13021 gagtatcaga ctctttataa aaggattact tcggacatgg aaaagaatgc gtcgaaaaag
    13081 tatgcaaact ctttagatgc tctgctgccc aaatcgctca ttccggtgat tataaagtta
    13141 tcgggaatag atcccgataa ggtatgtaac cagatttcaa gagaagaaaa aacttcattg
    13201 gtaaaactgc ttaaaaacct cacacttacg gtaacatcgg gtatggatat tgatgatgcc
    13261 gttgtcacaa aaggcggcat ttcggtaaaa gaaataaacc cttcaacaat ggaatctaag
    13321 atatcgccac gcctgttttt tgcgggagaa ataatagact gtgatgcact tacaggagga
    13381 tataacctga caatagcttt ttccaccggt tatttggcag gaaaaagtgt atatttataa
    13441 tcttttgttg taaaatgtgt ttttaatata tataattaac cgacaaagga agacgattga
    13501 tggaaaatga aacaataggc agtaaagata cttcaaaagc ggcaatcagg ctatcggttt
    13561 cttctaccag agaggaagaa tatgctttga ttaaagacta tgccgagcaa ggcattaaat
    13621 gtgttgctgt ggatttcgga ggcgacttca tatcttccgt ttccaagatt atcgaacggg
    13681 ccgtagttgc ttcaaaaaga caaaatgtaa tacgtgatgt tcaccatgaa cagggtgccg
    13741 tggtaggtgc cacccgcgaa gccttgggac agattatgcc caaagcgata ggttgcaata
    13801 tcgggggtaa gataggtatt gccagataca aggagcatat aaccgttgcg gtatttaccg
    13861 gcataggttt cttaaatctg gatgaagtag gaataggatt ggggcacaga agtatatgaa
    13921 gtataaatcc atagccattg acggtcctgc gggaagcggt aagagcctga tggcgagaat
    13981 gctgtcggaa caaaaaggtt atacatattt agataccgga gcaatgtatc gtactgtagc
    14041 gctgaaagct ataagaagcg gtatagatac tttggacggt gaaaaaatcg ctgaactgat
    14101 taaaaatata aattttaaat tggttataaa agataacaaa caatacaatt atctggataa
    14161 ccgatgtgtt gacggtctta tacgtaccaa tcaggtaagt aaaggcgcat cggatgtatc
    14221 caagtttcct cccgtgagac tgtttttggt ggagatgcaa agacaggtag ccaaagagca
    14281 taatgtcgta atggaaggca gagatatagg cacttatgtg cttcccgatg ccgactataa
    14341 gttcttctta actgcaagca atgaggtacg ggcaaaaaga cgtctgttgc agatgcaaca
    14401 ggaggggaaa accgtaacat atgaggaagt gctggccgat ataatataca gagataagca
    14461 ggactcggaa agagattttg caccgcttaa aaaagctgat gatgcaatag aaatagatac
    14521 atcaaaaatg ggaatagatg aggtttttgc aaccttgtcg gggtacatca atgggaaata
    14581 aatgtttcag aaaattttgt cgcggaatag taagaggaat agttaaaata ttctaccgtg
    14641 tcgagtatat cggtgaaaaa tatcctgaag aagggcctta tgtaatatac cccaaccatc
    14701 tgagcattct ggatatagta tttgtcattc tgcactccaa aaagagatgg gtttacttta
    14761 tggctaaaag cgaactgtat aaacacaagc tttccgcatg gtttttggat aagatgggtg
    14821 cctttcccgt aagaagaggg caatgcgatg taagaagcat aaaacgtgca gtgactttga
    14881 tacaggaagg taaacttgta tgcatatttc cgcaagggac cagacagaaa ggcaataaga
    14941 ttctgccgcc cagaagaggg gttgcaatgc tgtccgcttt aaccggggcg gaagtaatac
    15001 cggtgtttat tgaaaggaaa gccaagatac acaagaaaac aaaagtttat gtaggcaaac
    15061 ccgtcaacac cggtctggac aggaactctt ccaaagaaga gattgcaaaa aagagtgtgg
    15121 aacttatgga tatagtctac aacctgccgg aggtatataa agataagtaa gagagttatt
    15181 gttgcaaaaa atgccggcta ctgtttcgga gtttccaatg ccgtgaagat tgccggcgaa
    15241 ataacggaga aagaacagat aaaatattcg tacgggaatg ttattcataa ccatcatgtt
    15301 ataaaagaac tggccgataa aggactcata acggtagagg acatatcaca gataccggac
    15361 ggtgccggag ttctggtacg ggcacacggt gtacaaaagt cgttttttga acaggctgaa
    15421 gagaagaatt taataattta cgatgccaca tgtccttacg tcaggaatat tcaaaaaaga
    15481 gtggcggata tgtataacga cggctacaag attattattg tgggctccgc tgtacatccc
    15541 gaggttatag ggataaacgg ctactgcgac aataccgccg taataatcga gcaaccggaa
    15601 gatgcggaaa aattattacc cgatcgggaa aataaatact gtttagttgc tcagacaaca
    15661 tacataaaag aaaaatatga aaaaacagca caagagctta aaaaaaagtt tacattgctt
    15721 gaggtgtttg atacaatatg cagagctacg tccgtacgtc aaaatgaggc tgtaaaattg
    15781 gctgcaaacg tagatataat gatagttgtc ggtgatcccg atagctccaa tacgagaaaa
    15841 ctgtatgaag tgtgcaacaa tacatgtgca cggacatacc tggtgaccgg tgcggagcaa
    15901 gtgcgggaca tagatataaa aaacaaagaa acggttggta ttacggcggg agcaagtacc
    15961 cctgaccgta ttatcaggga ggtaaaggaa acaatggaag aaaatgcaaa gaagggatcg
    16021 gcggatactt ttgaggagat gctggaaaaa tccctcgtag tgttaagaaa cggacaggta
    16081 gtaaccggta aggttataag tgtcggtgag aaggaagtgt atctggatat aggcttcaag
    16141 attgacggca cgatacctgt ggaagaattc ccgactgtag acggtcaggt acaggtggct
    16201 gtaggggata aagtggaagc gatggtaacg agagtcagtg ataaagacgg tatcgtttat
    16261 ctttccaaga agagaattga tgaaaaaaga agatatgaaa atatatatga agcatttaaa
    16321 accggtgaac ccgtaaaagg taccgttaaa gaaacaactt cgggagggct tatactggat
    16381 attaacggta tggatgcttt catgcccatt tcacagataa gtacgcaatt tcagcgaaac
    16441 ctgcagaagt ttgtcggaag aaagcttaaa gtaagagtta ttgaatgtga ccgcagaaaa
    16501 agaaagctca tagtatccag aagggttctt attgaagagg aaaacgaaag aatggagcgg
    16561 gaagtttgga gcaaacttgc agtgggtgaa acggttaccg gaacggtaaa aagtttttcc
    16621 aacttcggag catttatcga tttgggcggc atagacggtc ttgcccatat atcggaactt
    16681 tcctggggaa agattaatca tccgcaggat gtactggaaa tagggcagga agtggaagtt
    16741 atgataagag atatcgataa agataaaaga aaagtatctc ttgtatacag aaaagaagag
    16801 gatgatccct ggtataatgc ggaagagcga tttgcaccca atacaatagt gaccggtaaa
    16861 gtggtcaaga ttttaccttt cggtgtattt gtggaaatag caaaggatat cgatgcactt
    16921 gtacacattt cccagatttc caacaaacgt ctgaagagtg cttcggaagt gcttgaagag
    16981 ggtcagatgg tggaagttgc aattatcgat gtagacctgg agaacaagaa gattaatgcc
    17041 agtatcaaag ccgttcagcc tctggatcct ctgcctacaa aggaagatct tgaaagagaa
    17101 cagctgagag aagaaagaag acagcaaagc ataaaggaac gtaccgaaag attcaaccgc
    17161 agggtagaag aggcggaatc ggcacaaaaa tccggagagc aaaggcaatc aagaaaacag
    17221 cgtgaagagc tgcccgtaag tcatgaggaa gtgttaagca ataagatagg ttcgttgctt
    17281 gcggatttca agtttgaacc ggaagaagag gataaaccta aagaagagga taaacctaaa
    17341 gaagaagata aacctaaaga agaagataaa taaacggtac gcggtactgt aataacggca
    17401 gtaataatta attgatagtt aacaaattga ttaataaaca aataaattaa tgcaaaggag
    17461 aaatcttttg catttttatt gtataattaa cagagggaga tattatggaa acaatagaac
    17521 agatgtccag agaaaacctg ctgatagttc tgaaatacag ggaacagcgc ataaaagagc
    17581 tggagaagga aatacagctt tatcagagaa taattaagga actgatggag aaaaaaaccg
    17641 ataatgaaaa aaaatgattt caagaatcat aatgagtata ttgagtatct gaagacaatg
    17701 aaagttgccg ttgtgggatt gggaataggc aatatcaatc tatttaagca tttggcaaaa
    17761 aaaggtgtcc atgttaccgg ttttgataaa aatgacgatt ttaccgactt aaggaagtat
    17821 atagaggaat atccccatgc gtctttaata accggaccgg attatttgga taaactggca
    17881 ggatacgatg ttatattcaa gactcaatcc atgaaaagag atttacctca gtttctggat
    17941 gcaattaaga gcggggcaac tcttacatcg gagatgtggg agtttataaa atactgtaat
    18001 gccaagataa ttgccgttac cggaagttcc ggcaagacca ctaccactac attgataggc
    18061 gagttgttga agcaacaggg atataaggta tgggtaggag gtaatatagg cacaccgcta
    18121 ttccacagac tggacgatat agacgtgaat gattatgtcg tactggaatt ggcatcctgt
    18181 cagttgcaga tgttttgcaa caagagtccg aatatttccg ttattacaaa cattacacct
    18241 aaccatctgg attttcatga cgattacgat gaatatattt actgtaaaag cattattttt
    18301 aagtatcaga aaagtgatga tatcataatt ctaaatcagg ataacgacat aacaagacaa
    18361 ttgagagaat cggtgcagtc aaaagcatat atgttttcca tgactgaaaa gtatgacaaa
    18421 gagaattcga taaacggtgc atatcttcat gataatgcca tatatgtcaa tattgacaaa
    18481 aacataaaga ggatgctgga tgttaatgat ataaagattc cgggaagaca taatatagaa
    18541 aactatatgg ctgccattct tgcgacatat tcgcttgtgg aacaagctac cgtaaagcat
    18601 gtggcaacta cctttaacgg agtggagcac agggtggaat atgtgaggac agttaacgga
    18661 gtgcgtttct acaacgactc cataggtacg acaccttcga gaaccgtggc aagcctgcat
    18721 tgttttgata agaaagtcat tttaattgcc ggaggttacg acaaaaacat atcctacgac
    18781 ataatgggac cgctcatcaa cgaaaaggtt aaggggctgg ttcttatggg acaaaccaag
    18841 aaaaagataa aacaggcata cactgattat tcctgcacag caacactgtg tgaagcgtct
    18901 acaatggaag aagctgtccg caaggcattt aacatggcaa aagaaggtga tataattata
    18961 ctgtctcccg caagtgcagc tttcgacatg tttaagaact ttgaagttaa gggtaatata
    19021 tataagcaga ttgtaaattc actataaata aagcaggtgt gtcatgaaaa aatacgtaat
    19081 tgcattggat cagggaacaa ccggcagcag agctgtcgct ttcgaccatg acggtaaatg
    19141 tgtcggttcg gtcaacaaag agtttactca gttctttccg tctccggggt gggtggagca
    19201 tgacccgtat gatattttac aaagtcagat gggtgcgctt ttcgagctta taaaaaaatt
    19261 agccgccaaa cccgaacaga ttgccgctat aggtattacc aatcagcggg aaacggttgt
    19321 ggtatgggat aaaatgacgg gaaaacccgt ttataacgca atagtatggc agtgcagaag
    19381 aaccgcaggt atatgcgaac agcttaaagc ggacggatat gccgatttga ttaaatccaa
    19441 aacaggactt gtactggacg catatttttc cggaaccaag attaaatgga ttctggaaaa
    19501 tgtcaaggaa gcaaaaaata agaaccttct ttgcggtacc atagatacct ggctcatata
    19561 caacctgaca aaggaaaaga gccatgtcac cgacatgtcc aatgcctcca gaaccatgtt
    19621 gtttaacatc aacacgttaa aatgggataa gcaactttta gatatattag gtataaaaaa
    19681 agaaatgctt ccgaaagttg taccttcttc ctgtgttgta ggtcatatgg aattagaagg
    19741 tataagtatc cccattgcag ggatagcggg ggatcagcag gcggcactgt tcggacaggc
    19801 atgcttcgag ccgggtgaag tcaagaatac ttacggaacg gggtgcttca tactgatgaa
    19861 caccgcggaa aagccggtat tttccgataa aggactgata accacaattg catggaattt
    19921 aaataataag acatattatg cactggaagg cagcatattc aacagcggtt ccgccgttca
    19981 atggttgcgc gaccagctgg gccttataaa aaccgcagcg gagaccgagg atatggcttt
    20041 tgcggtggaa gataccgatg acgtatattt cgtcccggca tttaccggtc tgggtactcc
    20101 gcattgggat atgtatgcaa gaggagtgct tgtgggtata acaagaggca caaacaaaaa
    20161 tcatatagca agagcggtac tggaatccat ggcatatcag acggtggatg taatagactg
    20221 tatgcaacaa tgctcaaagg tgccgttaaa gtgcctgaaa gtagacggcg gagcaagtgc
    20281 aaacaacttt ttaatgcagt tccaggccga tatgcttaac tgtgatgtgg tgagacctgt
    20341 tatgcaggaa accacctctt tgggagcggc tttccttgcg ggacttgccg taggcttctg
    20401 gaaggatgtt gaagaactga aatgcatcat aaagcaggac agaatatttg taccaaaaga
    20461 attctttgac agggcataca gaaaaataag atggcatgaa gccgtcaaaa ggtcaaaaaa
    20521 ctgggcgata gattagaaat agaaatcgtg attgcctatg cttttgtaaa aatcacaggt
    20581 atcggcaatc cagaaactct ttgccaattt aggattaaag aagaataaac accacgacac
    20641 attgttataa ccgttcaatg cgtcttttgc cgcacgtata gcttcttccg aagggtcatt
    20701 ataaattgtg ccggcagcta ccggtgtaaa ctgaacacct cccgccttat cgaatataac
    20761 gccgtatatc gtgtcgggaa aactgtcatg tcttacacga ttgagtacta catttgctac
    20821 cgccactttt ccttcatacg attcatgtgc ggcttccgca tgcactattc tggacaacca
    20881 ataaagatct tcataagtat aatttgcatt gaagtaaaaa gaatgcctgg taagcaatat
    20941 ggaatcggac acttcttcat ataaggtttt taagcctaat gcatcagcca taaccttcag
    21001 tgaagcatat atcttgccgt tgtatataaa gttcattgcg ggagattgta tcttgtgtcc
    21061 gttttccaga aggtaacatg tcccggaata aatctgatag gtattattac cgtcggttac
    21121 ggttacgctt ttcgtatcat tatcccactc atattcatat ctgccgaaaa agtcgaagaa
    21181 ttccatcacc ggcactatca gataaccgcc tactatcagt ttgtcattct taaactggat
    21241 attcttgtaa tcatatatca caggtgagtt ggtaacccaa tatttaatac cctgaggaga
    21301 tatctcggca cttgcatcaa cattgattga tattagcggt gagagcagaa tcattattgt
    21361 cagtatcgaa gttattattc ttcttagtct caaatctttc atcatttcgg tatattacac
    21421 caaaaaatac ggtaatgcaa tagcaaaacc gcaaattgtg aacaaattgt gaacaaaatg
    21481 tgaacagaga taataattag ttacattttt atataaattt taataaagag caatagagag
    21541 tgatagtgtc atcagatata ctgagtacgc catttttccc agaatatatc tttacctctg
    21601 gaggacatca tggcgcctat atcggctctg tttgctcctt ttgcaagcat atcctcaaca
    21661 atttcacaag cctgaatgaa accctcaaca acatttttgt tatgttcttt cagttcggta
    21721 atacaggaaa atactccgct ggctccgtca cgtacagaag cgaatacgaa gtccatggcc
    21781 agttcacgat ccagtaaatc gtgatatatt gaacacattc tccaaaggaa gggaactagg
    21841 cagttgttgt aaataattac agggtccagt tgcggggata ccgacggatc atatcgtgta
    21901 atacctaacg tttccagatt cttcaagtgt ttatacacca tgccttccga atgcaaagag
    21961 cgtcggttag cattgacatc ggaatagaaa atatccatat agggaataac aaattcctcc
    22021 cacatatccg caggaataag tgcagcacag tcatcggaca tatagggcgt atatttgtaa
    22081 tcgacattgt taacctgaca atagaatttc ttgaattgga ttatacttct gataagtttt
    22141 cccagaagaa cctttaattt ttccggctca tcgtaaatat cgtaatacag ctgcaaatcc
    22201 agcagggtat atgccgtggt tatcgggccc tgatatgcca tgccgaaatt tacattatct
    22261 atattcatgg cttttttcat attatcccta tactcaagat aaaacggaac catgccggtt
    22321 acggaaaaat ctataggttt atccaatatg cttatccatt catccagcga cttgtcgctt
    22381 cttgcataat taacttctcc gttaccgtcg ggaaaagcca gatccactcc cagcatattt
    22441 atatgaccgt aagatatggg cggggtagag ggactcagct tctttatttt ttcatgcata
    22501 ggaaaaaact cattaaactt atcgaagttc tcttttgaat acagttttac cgacgcttta
    22561 taatttgtaa acaaatcatg caatgtgaca ccggcaagtt ccgccattac ataataagac
    22621 atgttcacat gccatgtgaa cggagttttt tctcttaaag tggtgtagtc cggcttaaac
    22681 atatatgggc tccttcaact tttgtgcatt aatatttcct atcttattat acattaatat
    22741 tatataatta aaaccggaat gggctgataa gatgagaaaa gagcagaaaa taaggcataa
    22801 attcgcaaaa aaaaattttt tgaaaaattt tacaaaaaaa attaaaaaaa ggtgttgaca
    22861 taaattaact gctgtggtaa attgttaccg tacaaaggca aaactgtaga aatgcagtga
    22921 cgcaaaacta tggagcttcg ttgttagtta gcaagcccgc caagttgcta cattggacca
    22981 aacaggttcg gatgtagctt ttttattagg aaaataagaa agctgcaaac gggaaaaatg
    23041 tgaaatgtga cttaattgga tgaatgggcg agtgtaatgg ctaacgtaaa acaaactaca
    23101 actaaacaga atctccgtag tatggaaata tactatttac ccgtactcaa catacaactc
    23161 ggaataaccc aagcatatat cacccagtta aggataaatg atcctcagat gggtgttatt
    23221 attgatgaag catttatgcc cgtagccgaa agaaccgttg ttaccgaaaa gattacaacc
    23281 tggttgttgg acaacctttt caatgatatt cagagattca gaggcagagg agttcaagcc
    23341 gactggttcg gagtatacat tcccacccgc gtattgttga agaaagattt cttaacggaa
    23401 ttatgtaaaa aagtagttga atacaatatc caaaacagtg atttatgtat ttgtttgtat
    23461 aataaagtat tatatgaaga ccatatggaa attaaaagca tactttcaca agccaaatta
    23521 gagggtatta aatcccttat gttggacttc ggagatgatt tctgtcccgt atcaagaata
    23581 tccgatataa agaccgatta tgttcactta agtgagagtg taaccgaatc gttgatttct
    23641 aacgatgaaa ttaaaagaaa tacggcattg gggctgtatc atcttttaca ttctttaaat
    23701 atagaaacta tcacatctaa tgtaaaagat cgtgatgtat ccggtagtat tccggaaagc
    23761 tgcatattat gcacaggtaa atttgcaggc aattacagaa ggcccaggag cgtaagataa
    23821 tgagaaaaga tttatcagtt gctaaaaccg catacaacgt tcgcaggaca tcactctggt
    23881 tcagaggtat ggttatatcc ctgatagcct tagtattact cttatccgca gcttaccttg
    23941 tttcggcatt ctatgacaga tacagcagtt ttacggtatc cacggcatac agcggaagcg
    24001 gcagctccgg aaagggagga agcgattcga gaggcagttc cttgttgaaa ttagcattgt
    24061 ccttatgcga aactccggac tttcttacac ctacaaccag gttatatgca gatgccatag
    24121 agaactgcac caacatctca ggtcaatcca tacccgagaa tgttgatgcc attaacggac
    24181 agcataacgg agagaattat ttagcatata cgttctacct taagaacgca ggtatggaag
    24241 ccgtcacata cgaatatgta gtaaatatac tgaatgttac aaagaatgtg gatgaagcgg
    24301 taagaatcag ggtttatgta gacggaacac ctacaacata tgccaagacc aagacagacg
    24361 gaaccggccc tgaaaaggat actgtggagt tcttaaccga atccatagtt gcaagaaaca
    24421 gacgtgccta ttttatgccc ggtgacatta caagatttac cgttgttatc tggattgaag
    24481 gcgatgatcc cgaatgtttg gatgacatta tcggcggaaa gtttaaagtt acaatggaca
    24541 tagccattgt agaaggatcg taaaaataat gcttttcaaa aagcttattt aatattataa
    24601 taaaaaaagg agttattgaa gaaatgaaac aaatcaaaac aagacacaaa ctgatcccgg
    24661 ccatcataat gctgttggtt gctgcaatca caatgagcac agcatcatat gcctggttca
    24721 caatgagcac aaaggtagac atacagggta ttcaacttaa tgtaattgca ccggcaaaca
    24781 tacttataag agaaacaaag acattagcac cgtataatga gtttgttaat gtaattgaaa
    24841 ccacccaaaa tcccactaaa aagctaaaac ctgcttcttc cgtagacggt gtagctttct
    24901 tcataccgaa cgatgaaaca gcaggtaatg ttttagcatc aggagctcta ccggaaacag
    24961 gagagattaa cacaacatcc attccggtta ctaatgagca agacggctat tttgccgaat
    25021 acaaattcca gttggttaat accggcggta gtgctgtaaa tataggttta aaagacctgc
    25081 aaattacagc aggcggtgga aatcagcaag acacagatat attgcctgca gtcagatttg
    25141 ctatattgag tgaaggtgct aatgtaatta ccggagttgg tgaagacaag atatttgcat
    25201 ccagtgatac tgctacagta tatgctttat ctgctagcgg ttcttattca gggtcatcac
    25261 ctactacaac agaagcaccc gctcgtaaca agtttgatac aggtgcactg tttacacttg
    25321 ctgctaacgg tgtcgcaagt gcagtagcag acccggatgg tatgaaggat ataattgtaa
    25381 gagtttggat tgaaggacag gatactgctt gtaaaacaac aagtgcaaat tcgaccttta
    25441 aaatacagtt tgccttatat attataccct aactcttgat ttaactgatt tacctgcccg
    25501 tagcctttaa acgggctgcg ggcgggattg tatatctaaa tcggtataaa catttcagtt
    25561 gattgcgctg atttgtttaa tggaaaagat ctttcctttt gcttgaaaga ccttttctgt
    25621 tagatttaga tgtgctatta tatagttaaa tggggaggcg gattatgaag aaatggctaa
    25681 aaacggtagg gaatgtactt ttatgggtat ttattatatt tgctgtgttc atgacagtaa
    25741 ttgcatttac ctctaccaag aatgaacttg gggtctctgt tatcttaggt aagatgccca
    25801 ttaccatact gacggagtct atggagccga cactgaagaa aggtgacctc ataattagcc
    25861 atgagttgaa gccggaacag aagagcttat taaaggaaga cgatatcatc acatatgccg
    25921 ttgatttgga caaagacggc aagatggaac ttaacactca cagaattgtt tctgtaagga
    25981 aaggtgggga tgggtatata tactatacca ccaaaggtga taataacgtt attgccgata
    26041 ccgatgaagt acgttatgac aacgtaatag gtgtatataa cggaagaagg gttggcggtg
    26101 tgggaagtgt attgaacttt ttgcagacac cgaacggttt ccttatatgt gtagtcattc
    26161 ctttagtact gttcttactt tatgagatat ataacttcat aaaagttatg ttgacagtta
    26221 agaacgagaa gaagaataaa ctggatgaag aagagattaa gagaaaagct attgaagaat
    26281 atattgcaca gcagaataaa gaacaggaga agtcggagag cgacgctgat tctgcacaat
    26341 cctgaattaa acttaaataa aggatagatt taagcatgga tgcgttttta ggttggtttt
    26401 ttgaatttat gaactacatg ctacggggag tgatacgctt ctttgtaggc atatatgaag
    26461 gaatagtaca gacatttaat attgtaaagt acatcgagat tttcagaagc tattatcagc
    26521 aatttaagtt tctcgattgg ttctttgcca ttttagctat tattttggta atagcggtat
    26581 ggctgactat aatactgctt atcatcctcg gtataaagag gattataaac ataaggaaaa
    26641 atacagtcac acaggaaagt atctttgaag agataaccga tcttcacaga gaaatattgc
    26701 gacttaatga tgaaaaagac aggattttat ccttaaagat atcacaggcg ggtatatcgg
    26761 ctaaggatct ggccaaggag ttaaccgaca ttgcggagaa atcacgtgaa aaggtcagcc
    26821 agatggatga attcggtacg gcgactaccg attctacagg tgatatttcc gtatcttccg
    26881 atcagcagcc cgaagtaatg atggagtcca ggttctcgaa gcttattgaa gtggatcaga
    26941 aatatacata ttatactccg caagagtata caaccggatt cactctggaa cagctttgta
    27001 atgatttccg taacttcgca tgcagtaaga tgaagttgta ttacgagttg gaaatgatgc
    27061 gtgtcctgat ttcctctatg gcttccacaa aattgatagt actgcagggt atatccggta
    27121 caggtaaaac atcgttaccg tactgtttcg gaaaatttat taagaatgat gtcacgatgg
    27181 catcggttca gccttcctgg agagacagaa ccgaactgtt cggatatttc aatgagttta
    27241 ccaagaaatt caatgaaacc gaagtgttga gaagaatata tgaagcctca tacaacgatg
    27301 acatcaatat gattgttttg gacgagatga atattgcacg tatagagtac tactttgccg
    27361 aaatgctttc ggttttggaa atgcccgatc ccgagcagtg gcaactggag ttggtaccca
    27421 gttcctggac atccgacccg agaaatctgg taggcggtaa attgcggata ccggagaata
    27481 tctggtatct cggaacggct aataacgacg actctacttt cgcactttcc gataaagtgt
    27541 atgaccgtgc atttacaatt aactttgaat ccaaaggaca accttttgaa gcacccgata
    27601 ccgaagcacg ccctattagt tttacttatc ttgaggagct ttactccgat gcatgggaaa
    27661 gatttcccgt atccaaagaa aaccttgata agattgccaa actggattta tatattattc
    27721 agaagttcag aattgctttc ggtaaccgta ttttgaagca gttacttgag tttgttccgg
    27781 tatatgtggc atgcggcggc aaagagattg acggtataga ctatgttctt tataccaaag
    27841 tcttaagaaa attcgaaagc ctcaacctgt cgttgataag agatgacttg aaggagtttg
    27901 ttacatatct gaacagaagt ttcggaagaa acaatatgaa tgaatgtaaa aaatatctgg
    27961 aactgcttca gaggatgtat taatgatttt gtaggagagt attattatgg ccagacgcaa
    28021 gaaaaagtta atacttgacc aagagcagaa agattcaata tacgaagata caaaagattt
    28081 tatttcatac atatatgaag attatgactt ctatcaatcg cttgtacgtt tgttggacag
    28141 aggtaaaaac caggcggttc tttacagaaa ggtacttcag aagaatattg atgagatatg
    28201 ggtaaaaagg gtggaagatg ctttacctta tctggatacc gcattaaggg ctgtgaggaa
    28261 ggatataaaa gaggtggagg agatcaaacc catagcatta tccagaaaca taaccgttaa
    28321 gtctttgcgg catcttgcga cacataccaa ttttatagac agagtggatg aaaagggcaa
    28381 tgttataccc aacaagctgc tgaatgtata taaagatgag gaaatactta catatgagaa
    28441 taagttttta aacaccctca tatacaagtt ggctcttttt atagatgtca gatatgaaaa
    28501 actgaaagaa tacggtgcgg atgaagtatc caatgtaatg tccatggata ttgaaaccaa
    28561 ggttgtggat accgaactca aaatgagtat tcacatcgag acaaaagacg gagcggatga
    28621 acaggataaa aaggacaatg tagatattta cgaggatgtg tcaaagtctt ctctgtgggc
    28681 aagggttttg agaataagga agattataag gcagtatcag gcttcaccct ttataactca
    28741 gatgggaaag gcatatatac gtcctcccgt aatgagaacc aatgcaatta ccaagaatgt
    28801 gcatttgaga gagtgtctgg agttgtggtt gtttattgag agttacgaaa gcgccggatt
    28861 tgatatagaa agcagttcgg taacggaaaa accaagcaaa gaacttatgg agaatctcta
    28921 caggctgctg gcttttcagt atgttatgtt cagaaaaaat acatacgatc agttggatga
    28981 cataataaat aagagtaccg caaaactcag atacaatgcc cggtttaaac aattggatac
    29041 tcaggagttt accgatcagt atgagttttt aactcccggt gtcaaaaaag tatatgttga
    29101 ggagctgagc agaaaaggca ccagattgac taaagaagaa gtgaagatcc gtaaggctat
    29161 cgataatgcg ctgaaatcgg aaagactata tgcagccgaa aggaaaaaag ccgctcccaa
    29221 acagcgcagg aaaacaaagc aaaagtagtt tttcatgtgc tttcgggttt cggtatatat
    29281 acaaatatga aaaagctttt taaaagaaaa aatataagaa caattttcac ggtagtattt
    29341 tccgttttac tggttttgtt ggttcttctg gtgggtaata tcatttattc acagataact
    29401 cataaggttc ccgctatttt cgggtacagt gtcatgaata taattgccgg cagtatggaa
    29461 ccggttatac ctaaaaatac cttcatattg ataaagcagg tggatcccga acagttgcag
    29521 gtgggggatg taattacgtt ttattcttcc gatccgctta tacagggact tcccaatacc
    29581 cacagaattt ttgacattgt tatggagaac ggtcagaagt tctttgtgac aaaaggagat
    29641 gccaatgcca tacccgattc ctacaatgtc ggttttgatg atgttatagg ggtttatgta
    29701 aagaatatta tttcactggg taaaatcggt atgatttttc agaataaatc cgttgtattc
    29761 ctgcttcttg tcgtaccggc tgcagtgctg tttataatgg aaattatcaa tcttaccaaa
    29821 accgctgcca aggtaaagca ggaggatatt gaatcggctt caattgaaga aaagtgtgat
    29881 gatgttgaaa catccgattc aaaggaaaca gattcggaag atataaaatc agaggaaaag
    29941 gaggaaaaga gcgttgagtc aacctaagaa tgcggaaaat gtgattgaag agaaagacga
    30001 agatataata gaagaaaaag acgtagagac cgagcaaaaa gccgatgcgg ttgaagaaca
    30061 ggatgttaag aagaaaaaga aaggcttaat agagagattt cttggtccga aagagcccga
    30121 tgttaatgat gattccgatt atcagagtgt gctcaggaaa aacttttacg attttgacca
    30181 gattaataaa aagcggcaga taacttcatt gcggatggct gtaatggctc ttacccttat
    30241 tctgattgtc atactaaccc tttatatagt acatctgagc gattcgacga ttcttctgga
    30301 taaattaatc gattcgtcaa gtaaatatgt tttggataat gtgatgtatg tggataatcc
    30361 cataaaatat acagaagtgg tttcggaact taatgttatg aggactttga tgaatcataa
    30421 cggtgaactg ggaaaaagac aatatgctat aagcgagttg tattatatag caatacagct
    30481 tcccgattct tttgttaagt acaaagctga aatatatgat gcatttaaaa acattaatga
    30541 agggaaacag gatgtggggt ataagcttat agaggagctt ttggttaaaa tagtagagga
    30601 aagataggaa gtaagttgag aagaggtgta agttttaaaa gtgcatacct tgtcccttca
    30661 atgataatta tcatgtttct tctgctgttt gtaatgttat tccagatgcg tttttccata
    30721 acggagtttg taatggaaca ggctgcaacc gataatgaaa cgatagcaaa ggagaaatcc
    30781 gctttattcg attcattact ggtaaatatg tcattggaga caaacggaat attgaattat
    30841 acagacttta ccttttacac tatagagaat gaatatgatg ccagagaggc cataagcgac
    30901 tatttcccca acactaattg cgtgagattc ttttatttca tgcgtgacgg cagagtatat
    30961 gaccaggata caagacttta caaagatata gaggacaatc ttgcgcaatt gaaagccacc
    31021 gagacgcttt ctttgagtat gccgttttat gacgaggctg ctaaaaccga acttatcata
    31081 atttatttac ccaatcccga ttccggcagt gttcttgcag gtgttgccgc atactacaag
    31141 gtatcggaaa tatataccat aggcgagttc tatgtttccg acaccggctc gcactatata
    31201 tttgataaag acggtaattc cgtattctta aaagataaag gaataacggt agcatcactg
    31261 atggagagct atgaatttcc ggaaacggat attaacagaa ttacgaattt tatgcaggaa
    31321 aaaggtattg tagataccgt tatcagttcc tcggacagtg ccgtaataag tgtgtgcggc
    31381 gttcggaagt ttgtggactg gtatgttgtg tctttgctgc cgtcaacggt tatttccgat
    31441 aaaacttcgc atgttattga acaaacactg ctcatatccg tccttatcct tgccgtcttt
    31501 ataatcttcg tcatcatatt ccttgcagcg gataccagat ctttaagaaa aatggaaagg
    31561 ctgcagaata tcgatccggt tgcggaatgt gccaatcagt ataagtttga aatagatgcc
    31621 ggtgaaattt taagagcaaa ctcctctacc aaatatgcga tattgtatat aaatataatg
    31681 aatttcaaga atatcaaaca gttgttatca gtaagcgaag ttaatgcgat agtcagaaat
    31741 ctttcggata tattaagaaa aactatagta agaaacttag aaacatacgg tcgcacttcg
    31801 gacggcagga tggtagtcct tatgaggtat aaggatattg aagagataca ggatcggttt
    31861 aatgaaatag cggaagactt caaatcaacc gatccggtaa gcggatacat gatgagactc
    31921 aatgccggtg cttattgtat tgacagaagc ggcaattatc aggtttcgga aatgatagac
    31981 agagcggctt tcgctcagaa ttccataaag gaagattcca atataagtct ggatttctat
    32041 caccagaaga gtcatgacga ccttttaaga accgttgcgc tggaaggatt tatggaaaaa
    32101 gctttgagta atgacgagtt cattattcat tatcagccca aattcgatct taataataac
    32161 agaatttacg gggcggaggc actggtgcgc tgggtaagac ctaatgaagg aatagttccg
    32221 ccttctcagt ttattaacct ttttgaaaga agcggataca tttcacagtt agacagattt
    32281 gtttttaccg aagtgtgtaa attccagaac agaatggtta aagagggcag caggattata
    32341 cccatatcgg ttaacgtatc cagggcaagt gcggaaaaag aaggatttat cgaatattat
    32401 gtaaacacaa aaaatcagta cggtataaaa gacggttttt tggagctgga gtttactgaa
    32461 agtaaagcac tggaagataa cgatttgctg agtgacagaa taaaagagct gaagaagtac
    32521 ggttttgcaa tatccattga cgacttcggt gcgggatatt cttcgtttaa ggctttacag
    32581 actttggatt tcgatataat aaagctggac gcggtattcc tgaaaaccaa ccccaacaat
    32641 cccaagaaaa atgtcaatct tctctcgggt gttattgcac tggctaagac tttagggatg
    32701 aaggtaatag cggaaggggt ggaaactcac caggaagttg ccttactgag agaactgaaa
    32761 tgtgatgtta ttcagggatt tatatatgcc cggcccatga ccggtaccga ctttgttgca
    32821 ttcgttaaaa acgctctcag taccagaacg gaaaaagaca tgctgacaga ggatattacc
    32881 tgatatgccc agaataatag cgggaagcag gaaaagtata atattggaag ctccgaaagg
    32941 ggagaatgtt cgcccgacat atgacaggac aaaagaaaat ctgtttaata ttatatcttc
    33001 aagatatcct ttggaaggtg catatgtcct ggatatgttt tccggaacgg gaagcatagg
    33061 tctggaatgt ctgtccagag gagcaaagta tgcggtgttt atagacaaag atgtttcact
    33121 gacaagaaag aatattgaaa aatgcgattt ttccgatgtt tcccgtaccg taagaggcga
    33181 ttgggcgacc ggattaagaa tattaaaaga ggaaaatatc aagtttacgc acatttttct
    33241 ggaccctccg taccactcgg gactgcatga aagaattatt gaaaacagtg atttgtatga
    33301 tataatgggt gaaaacggtg ttgtaattgc agagttgtcg gacaaggcaa atgttccgga
    33361 aaataccgcc gaacttgtac tcaaagacaa aagaaagtat ggaatctgcg tattcgcatt
    33421 ttatattaag gggagccatg aagatttgga tatgtccggg gagctttgat cccgttacca
    33481 aaggacacat agatataata tcaagagccg gtgtaatgtg tgacaaactg attgtcgcag
    33541 taggcgaaaa caaattcaag aaaagtgtat ttacggccga agaaagagtg gacttcataa
    33601 ggcgttcttt aaacggattg aaaaatgtgg aagtggaaag ttttcacggt cttttaattg
    33661 attatgcaag agagaaaaaa gctatatgca tagtaaaggg tttacgtgcc gtatcggatt
    33721 tcgagtatga aatgcagatg gctttcctga ataaacgcat ggacgaggaa atcgagacgg
    33781 tatttctgat gaccaatatc aattattctt tcttaagttc gtctgcggtc aaggagctgg
    33841 ctttacacgg aggtcccatt gaaggactta tacccgatgc tataatagat gatgtaagga
    33901 aaaaattctt taaataatgt gaggggtaac ataatggata atactatgaa cgaatatgag
    33961 ataatcgaac agatggaaga ccttgtaaca agcgcaaaac agacggtttt ttccgacaga
    34021 atatccataa gcaaagatga gatgctggac cttatttact atcttaaaaa tgctataaaa
    34081 cagcgggatc aggaagaaga ggcaaagaac aaagagttgc ttcgtatgat ttccgaagcc
    34141 aagaacaggg aatcggatgc cgatgaccaa agcgttgtcc tggaggatgc caacagagaa
    34201 gcaaacgaaa tcaagagagc cgcactgaaa tatgccgata atttgcttgc ctcggttcag
    34261 gaaagtattg aaaaaaccct gaacattatc gcctccaaca gagaggaact ggatcagtag
    34321 ttaagcagaa tacgcacatt tgacgatata caaattattc cgacatatac aataaacaat
    34381 atattttaca aacctttttc tttcctgctt ttttaagaaa actgttggac agcaaagaaa
    34441 aatgtgctat tttaatggtg tgaaaagtag aatttgttga ggaaaaggtg tcatgaataa
    34501 agcaattgcc aaggccggtt caataatagt aactttaact gtaattcttt ttgcaatctt
    34561 tatgtttact tccgaatttg ccagttattt agtttgcata tttcttgcat tgggatatct
    34621 tatgatgatt gcgggatacc ataatgagag cagtgatgac aggaaagcag ctgcaaatgt
    34681 cggactgata ttcggatgca tatatgccgt ttttattatt cttgtgtact ttgcacagac
    34741 aacagctgtc agaacggatt ctttgaatga acaggctatt atgattcttg attattccaa
    34801 aggcggattg tttttcagtt atgatttgct tggttacgga atgatggcat tgtcgacatt
    34861 ctttatcgga cttagtatta aaccgaagaa taagacagat aaatggctta agtatcttat
    34921 gatgattcac ggagtatttt tcttgccgtg cttgattatg cctatgacag gagtattcgg
    34981 ttcatttatg gcggatgatt caagcagcat aggcggaatt attgcattgg agttctggtg
    35041 tgcatatttc ctgccgatag gtatattatc gttttttcat ttcaaaaatg attaaattaa
    35101 aaatcttgaa tcttgctgta aagtgatgtt cttctttaag tgttgatata aggtatttaa
    35161 tttgttatgc aaaattgata taatcggatg tatgaatgat ttttgcaata agtatccatc
    35221 cgataatatg cttttgtata atgccgatca atattgccgt aagattgcat caattaatta
    35281 cggaaaaaat gcaaaatact ttatagaaac cttgggatgt cagatgaatg aagcggacag
    35341 cgaacagctt gccgggatgc tgacagacat gggctacagc caagcggagg acatttcctc
    35401 tgcggatatc atagttatca atacctgctg tgtcagagag aatgccgagt ttaagcttta
    35461 cggccattta ggagagttga agattctgaa gcaacggaat aaagaattgt tgattgtact
    35521 atgcgggtgc atgatgcaac aaagtcatgt ggtggacaag attaagaaca gctataaata
    35581 tgtggatatc ctattcggga cacataacag acaggaattc cccgaacttc tttttgacag
    35641 tattagtgaa aagaaaaaaa tatatgaaat atgggaagaa agcagaggta ttgccgaaaa
    35701 tgtaccggta aaaagaagca gtacaattac ggcgtatgtt ccggtaacgc acggatgcaa
    35761 taatttctgc tcttactgca tagtacctta tgtacgggga agagagcact ccaggcatcc
    35821 cgagataatt aaaaaggaaa taattcagct tgcttccgaa ggtataaaag aaataacgct
    35881 gttgggtcag aacgtcaatt catattactg cggtatggat tttcccggtc ttttgcacta
    35941 tatatccgat attgagggaa tagaaagaat aaggtttctg tcctgccacc cgaaagacct
    36001 tagcgatgat ttgataaaag ccatggttga aatacccaaa atgtgttcgc atattcatct
    36061 acccctgcaa tcgggaagca acaaagttct gcaacagatg aacagaaagt atactaaaga
    36121 gcagtatctt atgcttattg acaagataag agcatcaata aaagacattg cgataagtac
    36181 cgatattata gtgggttttc cgggagagac ggaagaagat ttcaatgaaa ctctggaagt
    36241 atatgaaaag gcgggttttg attttgcatt taccttcatt tattcaaaac gcaccggtac
    36301 cgctgcggca aaacgaccgg atcagatacc cgaagatatt aaagacacca ggtttgcaag
    36361 gcttgtagcg cttgttaacg atatgacact ggcaagcaat atcagggatg agaataaggt
    36421 tttacaggtt cttacgggag ggaaaagcag aaccgaccga tcggtaatga cgggcaggac
    36481 aagcagtaat aaaatagtta attttaaatg tgataacgat tttacaggtc atcttgttga
    36541 tgttaaaatt aaaaaaggcc atacatggta cctggaagga gaaaaagtat gatatttgat
    36601 aaagtagatg aattaagtgc attgattgag gatacaaaac agtatgacga ctatctggaa
    36661 gcggaagtgg agttggagaa tgacggtcac ggtatagata tgataaatgc cttctatgat
    36721 ttgcagcagg aatatataaa gacatacaat gaaggcagtt atgatgagat attggctatg
    36781 gagaatgtat tggaaatgag acataccgag cttatgaact acactaccac aggcaattac
    36841 ataagagcaa aagaagccct tgatgagctt ttacagcaga taaccgacag aataatgagt
    36901 aatctcggat tggatacgga ttgcgatgat gactgtgatt gtgattgtga tgaatgttct
    36961 tatggaggac atattaacta aatggaaaat ctcacaccta tgatgaaaca gtatatgaag
    37021 acaaaggagc agtacaaaga ctgtatactt atgtttcgtc tgggcgattt ttatgagatg
    37081 tttttcgatg atgccgttac ggcatccaaa gagttggaga taacgctgac aggaagaaac
    37141 tgcggtctgg atgagagagc acccatgtgc ggtgtacctt atcacagtgt agacccttat
    37201 atagccaaac ttatcaaaaa aggatataaa gtggctatat gcgaacagct ggaagatccc
    37261 gcaacggcaa aaggtatagt taagagggat gtcacaagaa taataactcc cggaacggtg
    37321 cacgagaaca cgctactgga cgaaaaagag aacaattaca taatgtcgct ttttaaaagg
    37381 ggtgaggtgt actcattggc ggtatgcgat gtttccacgg gggagttgaa cagctgccag
    37441 attatttacg gaaatacgtt ggaaaaactc ataaacgaga ttgttaaata caaacctaaa
    37501 gagataatat ccaatttcga taccgagttt ataaagcagt atataagtgt atatgtttcc
    37561 gatgtggaac agtcattgtt cgaccgctct tttcccgata tagccataaa gagtaagagc
    37621 aagaccaatg actttgatat ctggagaaat gcctgtgcgg ctctttatca atatcttaag
    37681 gagacgggaa agtgtgatat ttcccatata tcttttgtca atgtctatga agccgaacag
    37741 tatatgtctt tggacttaaa cgcaagaaac aatctggaat taaccgaaaa cctcaaagat
    37801 aacagaagat acggcacgct tttatgggtt ttagataaaa catccacggc gatgggtgcc
    37861 aggcttatta aaaaatggat tgagcagccc ttgaatgata tagatgatat taatttgcgt
    37921 ctggattcgg ttgcggaact gaaagataaa tacataatgc gtcaggaatt aatggagctt
    37981 ttaaagagtg tttacgatat tgaaagactg gtttccaaga cggtaatggc aaatgccaac
    38041 ggcaaagacc tcatatccct taaaaactca ctgtttaaac tgccttatat aaaacaactg
    38101 tcggtaaagc tggaaagcga cttattgaga catacggaca gcaatctgaa taccttttcc
    38161 catctagccg accttataga acaaagcatc aacgaagagt gtcccgtagt attgcgtgag
    38221 ggcaacctta taaaatccgg ttttgataaa ggggtggacg aattgagaaa agcctctcac
    38281 ggcggtaaaa actggataat agagtttgaa gaaaaggaaa aagagagaac cggtattaaa
    38341 aagttaagag tttcatataa caaggttttc ggctactata tcgaagttac caacagtttt
    38401 ttggatcagg taccaactga ctatgtgagg aaacagactt tggtaaacag tgagagattt
    38461 atcacacagg aactgaaaga tatggaggaa tccatattgg gagcggaaga gaagctcata
    38521 agactggaat atgagatatt ctgtaaaata agggatatca ttgcttccga tgccgaaaag
    38581 ctcttaaaaa cggcttacag cctgtctgta ctcgatgtgt tgtgttcttt gagtgaagca
    38641 gccgacagat ttacatataa cagacctgaa atgacaaaag attgcgttat agatattaaa
    38701 gagggcagac atccggttgt ggaaagaatg cctacctgtc ccgagttcgt tccgaatgat
    38761 ttatacataa atgataccac ggacaagact ttaattataa ccggacccaa tatgtcgggt
    38821 aaatccacat atatgcgtca ggcagccctc ataacaataa tggcacacat aggttccttt
    38881 gtgccggcac agtcggcgac aataggtata accgataaga tattcaccag gataggtgca
    38941 accgacgatc ttgcgggcgg caagagtaca tttatgatgg agatgagcga gctggcatat
    39001 atcttggaaa atgcaaccga taaatcattg ctgatattgg atgaaatagg caggggaacc
    39061 agcacatttg acggattatc cattgcctgg gcggtaattg aatacttaaa tgaaacaagg
    39121 gcgttaagaa cactgttttc cactcattat catgaactga cagtactgga agataagtta
    39181 tcgggtgtaa gaaactattt catatctgca attaaaaagg ataaagatat agtgttctta
    39241 cgtaaggtta aaagaggagc ggccaaggaa agttacggta tagaagtggc ccgtttggcg
    39301 ggtattcccg agcaacttgt ggtgcaagcg ggggaattcc ttaccgaact ggaaaagagc
    39361 gagaataaac gaacaaggat aagaaataaa gagacatata tggaaggtca ggttggaata
    39421 ttggagttta ataagcggga tgacaattat aaagaaataa aagacatact ggaaaactgc
    39481 gatattcaga agatgacacc gttggaagca ttgaacaaat tgtatgaatt aaagagtaag
    39541 gtataaaatg agcggaaaaa taattatact tgatgagttt acatccaatc agattgcagc
    39601 cggagaagtg gtggaaagac ccgcttcggt tcttaaagaa ctcattgaaa actccataga
    39661 tgcaggtgcc gataaaatag agattgagat taaaaggggc ggtataggat acattaaagt
    39721 atcggataac ggtaaaggaa ttgagccgga agacattccg atggtatttg aaaggcacgg
    39781 gaccagcaaa atccgtcaaa tttccgattt gacgaccatc tccacaatgg gtttcagggg
    39841 agaggcgcta cccagtatag catcggtatc tcaagttaca ctgactacta aaacccagga
    39901 taacgaatat acaacaaagg cggtatatga agcattacag caaaaaagca ttgtaaaagc
    39961 cgccagagct gccggtacaa ccgtggaagt gagaaatctg ttctacaata caccggcaag
    40021 atacaagttc ctaaaaggga acacccagga agcacgttat tgcaccgata ttgtgctaag
    40081 actggcattg gcaaacgaac atgtcgcttt taccatgata tccgataaca gggaagtgct
    40141 cagaacaacc ggtgataaag atcttttaaa caccatatat tccgtatacg gaagagatat
    40201 agcaaaagat ctcataaagg ttgatgccaa gtccgataca ttcagtgtag cagggtatat
    40261 aggtaaaccg ttcgcgtcca gaggtaacag acaacatcag agtttaatta tcaacaacag
    40321 atatgtaaaa tatccggaac tgaacgccgc aattgaaaga gcatatcaga ctcatgtgat
    40381 gaaaggcaga ttcccgttct atgtgttaaa actcaatgtt cccgctcaca atctggatat
    40441 caatgtacat cctgccaaaa ccgaagtcaa aatatccaat atatccgata tattcggaaa
    40501 acttaacttc ataataaaag aggctttgga taaagaacaa caggcattta cacctgcaac
    40561 ggtaaaacct tcatatacaa aaccggagat aaagaaagaa gacggcaacc tgataaaaga
    40621 ttttacggaa cagctggcaa aattaagaga agaaacaccg ccggtgacac caaaaacaca
    40681 tataatcaat gaacagataa ccgtctataa agaacagcct gttgtaaata caagtgatat
    40741 taagcaggaa tttactgatt ccgttgcaga taaaatatat tatgatgaac cgtataaaat
    40801 aatagggcag gtgttcaata cctttataat agtggaaaaa accgactata tttacataat
    40861 tgaccagcat gcggcacatg aaaagatgtt tttcgagtcg cttaaggcgg attatgataa
    40921 acaatccgtt atatcacaac agttaatggt tccgacagag gttgtaatga caagtgttga
    40981 gatgagtttt ctggagcaga attcggaatt gattaaaaga atgggatttg attactctca
    41041 attcggtaaa aactcaatat taataaggga aattccctca acaataaacg agtgtgatat
    41101 aaaagatgct ttcgcaacca tgctggatga catgcagagt gaaaaaaagg ataaggtaaa
    41161 taccgcaata tattctttag cctgcaaagc tgcggtaaag gcaaacagaa aactggatga
    41221 aaagcagatg cggtatttat atgaaaattt aatgaaactg gacaatcctt tcacatgtcc
    41281 gcacggaaga cctacaatag ttaaaatgac acgatatgat tttgataaac tgttcaaaag
    41341 gattgtgtaa tgaaaaaagt cattatcata accgggccta cggcatcggg taaatcaaaa
    41401 gcagcattat ctcttgcgaa gaaaatagac ggcgaaataa tatcggccga ttctatgcag
    41461 gtttataaga gaatggatat cggcaccgct aaaccgacaa aaaatgaatt agatgaagta
    41521 aagcatcaca tgatagatat atgtaatata gatcaggatt attcggttgc acaatataaa
    41581 gagcaggcaa cagacagcat taacgacatt atatatagaa agaagacccc tataataacc
    41641 gggggaacgg gactttatct ggattctttg atatataaca ttgattttaa gcaagagcgg
    41701 caaagcaaca gagagtatta tgagcaatat ctcaagaata acggcaaaga tagtctatat
    41761 aacttactat tacaaagaga tcccgatgcc gcattatcga cacatccgaa taatacaaaa
    41821 agagtaataa ggtatttaga tatactcagc ggttttgaag gaactttgga agagtataaa
    41881 aaagctgcta taggtaaaaa tactgacttt gatttccata tatacctatt acatatgcaa
    41941 agagagcaac tgtacaggag gattgagaaa cgggtggatg aaatgcttga tgcagggttg
    42001 gttgaggaag taaaaggtat tctgaatgac ggctattcta cagattgcaa cgcattaacg
    42061 gcgatagggt acaaagaaac agcggaatat ttaagaggtt tttgcactta tgatgagatg
    42121 gttaatatac ttaagaaaaa caccagaaat tacgcaaaaa gacagataac gtggtttaaa
    42181 agatatgaag aagcaaggat aatcaatatt agagattata tagatgccga tgatatagcg
    42241 gaatttataa gagaggatgt aatgagaaca atgttaaaag aagaaaaaaa cgaaaaagtt
    42301 tctccggttg cgaaaataac gggtatattg gaaggcaaag tggagcctaa tgtagataaa
    42361 gaagatataa agaatgagag attatcagaa agatacgtta aaaaatcctg aaaaccccga
    42421 ataccttacc taaaatctgt aaatcattgt atacataaat aggttcatag ttgtcatttt
    42481 ccggttgcag acgtactctg tcgcgttctc tgtagaatgt ctttaccgta gtttcattat
    42541 ctataagtgc aactataata tccttgtcat atgcaacttc ctgttttttt accaaaacca
    42601 ggtcattatc atgaataccg gcattaacca tgctgtcgcc tttgactttc agcatgaatg
    42661 ttatgccgct tttaagatat gcttcgggaa cggggaagta ctctactata ttttccactg
    42721 caagaatggg tgttccggct gcgacatctc ctattaccgg aacttccact accggtacgg
    42781 catcctgttt ggaaagtgtc agagaacggg aaatcgagtc atttttctca atgtaaccca
    42841 aacgtctcaa tttatttata tacgaatgtg cggtagatgt agacgggata ttcaagtctc
    42901 tgcatatatc ccttaccgaa ggggcataac ctttctcacg aatgtaatcc cggatatatt
    42961 cgtaggtctg ttcctctctg attgacagat aatctttcat ttatttatac ctccgctgct
    43021 ttacaggtag attatagcat ggatagggag cggatgtaaa caaatgtttg agttgcggat
    43081 atatataaga tttgatagat atgacgtgat gaataattat tgcaaaagtg tcaagtaaaa
    43141 cactgcaaaa gtgtcaagtg agatactaat ctgcataatt agttttcttt agattgttgt
    43201 aatctttgag tttttgttca tcgagataca gatggcgtat tctttttaaa ctcaataaag
    43261 gtgttacatc atcaaattga ttattacata aatctaaaac ttcaagattt gttaaattct
    43321 ctaagaattt caaatctttt actccggtat tattcaatgt aagcaaagtc aaatccgtta
    43381 actgccctat cggactaaaa tcttctatag ggttattatc taaacctaac cagtttagtg
    43441 tttttaattg tagaagaggg cttatatccc ttatatttgc atatcttaca gtaaaatgct
    43501 ttagattagg gcaatttact atcggactta tatcacttat ttttgttaag gaaacaattt
    43561 ccagcgaagt taaattctca aagtatttca agtcttccaa cgaagtgatt cttgttattc
    43621 tgtcgttctc aacacctcca cagggaccgt cttcgtttat tgtcagaatg taaggagttt
    43681 cttcgatatc attatggtta aaaaaactgg gatatatcaa tatactttcc actttaagca
    43741 tattctcatg tgtaaattcc catcctttct cgcgaaatcc cgatttcaaa attctttcaa
    43801 acagagcgtc tgcaaacagc gggcgtaata tatatctgta ttggttgttc tttgcatatt
    43861 catgagcata ggagttttcg taaaccaata aggtaatgtc ggaatatgca tcaaacattc
    43921 tttcctcaat atatttaacg gatagaggaa tgtatacata accgggtttt tccaaaatag
    43981 taaaatcttt tttgtttata ctttctattc cttctctaac atatatggta gacagacttt
    44041 taagactttc gcagcccata aacacatcat ctgcaacgta tttaatgttt ttaggtaaga
    44101 ctatggaatt tgctgtaccg tattgataat ctctctgctc attatcatat ccgtttatgc
    44161 gaatttcata caaagaacta ccggcgaatg cctgctcaca aatggtatga atattgtcat
    44221 gaagtagtat tcttaacagg cccttgcagt tgtagaatgt ttttttatgt attgtgtcta
    44281 cacccgtggg aattatgaag tcatcaccta tgcttgttcc ctcataggcg ctttccccga
    44341 tgtaattaac ttgttcaatg ggataaacac ttttcaatgc taaacagtcc ttgaaacagt
    44401 aatcgggtat tattgttatg ccttcatgca gtttaatagc ccaaatatca tttctatccg
    44461 tgaatgcaca agcagacagg tatttgacgg ttttgggtat ctcaacattt atttccgtcc
    44521 cgttatactt aatcagcact ttgtctccgg caattacaaa tttatcatta agattttcat
    44581 accacttagt tcctttaaat gcgttatagc ctatgtgcga tacggtatcg ggtatggtga
    44641 tattaataat atccgtcatg ttcatgaaaa cttcattatc aatgccttta accggatatc
    44701 cgttaatagt atcaggaatt actacttttt ccgtgccttc catattcttg tattttgtaa
    44761 tcattaccga agaatcttca ataatgtaat caaatatatc attatcttct tcagtttgat
    44821 tagaacatcc gcatatagtg agcaggcata gaattgttat taaaacggtg ataagtttat
    44881 acgatatttt tctattcatt ttaagtgcac cctttacata gttgtatccg taagtttcgt
    44941 atttgtgttc attagacgat ttgtataaca gaaatgtttc atgtagatta tagcatgctt
    45001 tatggagttg acgacagcct tttcccttgt aacacaaaaa gtgcaatggt ataatcatta
    45061 ggatgaaagg aaatttacta tgaaaaaaac aatactctct ttaaactgcg gaagttcttc
    45121 actcaaatac aatgtattca gaatcaataa tgatttgacc gttacaccta tactgaacgg
    45181 taatgccgaa gaaataggca atccccaaag aagcaccatg acttttaaga taaacgatga
    45241 aaagaataag tatccggtac cgttggaaac acatgaagat gccatccatg agatgattaa
    45301 agtcttcagg aaaaacggaa taaccaaaga gagcatatgt gcagtaggtc acagaatagt
    45361 acacggagga ccggtatata caaaaagtac cctcataacc gaagaagtaa aaaaacagat
    45421 aatagaatta ttttccgtcg ctccgcttca caatccccag aattatatcg gagtggaaga
    45481 aatagagaaa ctgcttccgg gcgttcccaa tgttgccgta tttgacacgg catttcacgg
    45541 aaccatggga gaagaagctt tcaggtatgc cgttcctgag aagtggtata aagagttttt
    45601 agtaagaaga tacggtttcc acggcaccag ccatctgtat gtctcaaaaa gagcggctgt
    45661 tctgttgaaa acaccttata atgagtttac ctgtattacg gcacatctcg gcaacggatc
    45721 cagtataaca aaggtggaaa acggtaagtg cagagatact tccatgggtt tcacaccgct
    45781 tgcaggtgtc ataatgggca caagatgcgg tgatatagat cccgccataa tcggtcatgt
    45841 cgccgctaaa tatgcatcgg aaggtatgga taaatacgaa gcatttgaaa agaccgtaaa
    45901 aatgcttaat aaggaaagcg gattgatggg aattgcggga accaatctta tgcaggacat
    45961 ccgtgctaag gctttggaag gcgatgagca atgcatgaag atagtggata tctattgcta
    46021 caggattgcc aaattcatag gcgaatatgc ctgcaccacc gataatctca aagccattgt
    46081 ctttaccgca ggcgtgggag agaatgagtg gtttataagg cagagagtat tggaaatgtt
    46141 aaaggcgttt gcatttgaaa tagataatga agcaaacagg ttaagaggag aggaagtaat
    46201 tgtcggaaaa ggccgtttcg caggtaacga tgtatatgca atggtcattc ccaccgatga
    46261 ggagctggtt atagcttatg acactcttgc aataggttac cttggggaaa aggcaccgac
    46321 ggtatatccg tttgaaaaag tttaaaacga aagacaattt taatagattt tttgaaaagc
    46381 acttatcagt tattgatttg atacatgaat gcggctataa agcatatttt ataggcggtt
    46441 gcgtccggga tgccgtaatg ggagttcagg cttccgattt tgacatagcc acaaatgcaa
    46501 caccggaaga tattattaat atgtttaccg atgtcagaaa aaccggtata gaattcggta
    46561 ctgttacggt aaaaaccgca acgggtagct atgaaattac cacattcaga aaagaatacg
    46621 gttattccga caacagaaag cccgataagg tagtttacgc aaaaagcctg tatgatgatg
    46681 tgcgaagaag ggattttact gtaaatgcat tggcatatga cggtaagtat gtttacgact
    46741 actttaacgg tatagaggat attcaaagga agattataaa aacggtaggt tcaccgaaaa
    46801 gacgcttcag cgaagatgct ttaaggatat taagagcggc aaggtttgca tcaacattgg
    46861 atttttctat tgaagacaag acttcggaaa gtatgaaaag atgtgcacac cttttacggt
    46921 ctatttcaaa agaaaggatt ttcagtgaac tttccaagat gatagcagga ggagagagct
    46981 taaaaatact gtttgatacc ggcattgcag actatatttt cctttatcct catcatatca
    47041 atcaggataa tcttaaaaaa ggctcatata aatgcagact ggcggatgtg ttttgcaaat
    47101 atccgagtgc ggatatggcg gttgaggaac ttaagaatct gagggcggat aataaaacgg
    47161 tcggtacttt gtatgatata ctcatattcc tgaattccga taccgatgaa atgtctgtaa
    47221 gaaaattgct gagcgagata gggttggaca atgtttcggt tttattggaa ttttcaggac
    47281 aatcgaggga tgttttggac aaaatagtta aggaagggta tataaccggt attaatgaat
    47341 tggcaatatc cggtcatgat ataatggcga tgggatatcc caaagagagt attaaagata
    47401 taaaatggtt tctttttaat aaagttattt atgatatttc tcttaataaa aaggaaatat
    47461 tacaagattt attaaacaat tgcccgattg aaaagcattg actattaaga tataattttt
    47521 gcgaaattgc agttggagag taatttgaaa aaagacagga taagaaattt ttgtataata
    47581 gcgcacatag accacggcaa aagcactctt gcagacaggc ttatagaaaa aaccggatta
    47641 ctgacaagca gggaaatgtc cgaccaggtt ctggataata tggatataga gaaggaacgc
    47701 ggtataacca ttaaatccca ggccgtgcgt atggtataca agcataacga cggtaaagaa
    47761 tatattctta accttataga tacgccggga catgtggatt tttcttacga agtcagcaga
    47821 tcgcttgccg catgtgacgg tgcaatactg gttgtggatg ccgctcaggg agtggaagcg
    47881 cagacactgg ctaatgtcta tatggctatt gataacaatc tggaaatact gccggtaata
    47941 aataaaatag atcttccggc cgcaagaccg gaattggcta tagaagagat agagaactta
    48001 atagggattg aagcgcatca cgcccccctt atttccgcaa aaaaccgtat aaacatagaa
    48061 gaagtgttag aaaagatagt gacatatctt cccgcaccat tagataaatc cgatatgcct
    48121 gcaagagccc tcatattcga ctcgaaattt gatgcatata aaggtgttat aatctactgc
    48181 agagtaatgg aaggggtaat aaaggctacc gataccattc gtctgatgaa tacaaaaaag
    48241 gagtatcagg tggtggaatt gggttatctt aaacccggag gctttatacc cgcggagcag
    48301 cttacttcag gcgaagtggg atatattact gcaagcataa agaatgtgga agataccaga
    48361 gtgggcgata ccgtgacaac tgcggagaat aaagcggcag tgcctttacc gggttacaaa
    48421 gaagcggttt ccatggtgtt ttgcggtatt tatcccgcag acggcgccaa atacaatatg
    48481 ttgaaagatg ctctgatgaa attaaaactc aatgatgcgg cattgatgta tgagccggag
    48541 acatcggcag cattgggatt cggatttcgg tgcggattct tagggcttct tcacatggag
    48601 attattcagg aaagaattga aagagaattc gatctggata taatcaccac cgcacccagt
    48661 gtcatataca gagtgacaaa aaccgacggg caagtgctgc gagtggataa tcccaccaat
    48721 cttccgcctg taagtgaaat tgcctatatg gaagaaccca ttgcaaaagc cgttataatc
    48781 gttcctgccg aatatgtagg taatattatg gagctgtgtc aggacagaag aggtacattt
    48841 attcatatgg aatatattga tgccacgaga acaaaacttg attatgagtt gcctcttaac
    48901 gagataattt acgatttctt cgatgcgtta aaatcccgtt ccaaaggtta tgcatcatta
    48961 gattacgaag ttatgaaata tgagaagagt aaactggtca aattggacat attgttaaat
    49021 caggaacttg tagatgctct ttcttttata gtgcatgaaa gtaaggcgta tgcacgcggc
    49081 agaaggatgg tggaaaaact taaagagcag atacccagac atcagtttga aatacccgtt
    49141 caggctgcaa tagggggtaa gataattgcc agagagactg tcagagcata cagaaaagat
    49201 gttatagcca agtgttacgg aggagacatc tccagaaaga agaaactgtt ggagaaacag
    49261 aaagaaggca agaagagaat gaggcagtta ggttcggtgg aagtgcctca ggaagccttt
    49321 atgagtgttc tgaaattgga tacctgacat gaatgatttg aggatataca ttcacatacc
    49381 gttttgtaag tccaaatgca attactgcga tttcaattct tttgcgggta tggaccatat
    49441 gttccgaccg tatttcaaat cccttataac cgaaatagcc ctttattcca atgaacttcg
    49501 aggcagaaga atagacagca tatatttcgg aggaggaaca ccctctcatg tggatgtgca
    49561 atatatctat caggtgttga aatgttttac atttgatgaa aataccgaaa taactattga
    49621 agtcaatccc ggtgttgtgg gttaccataa tttcgtacaa tacagacaga tgggaatcaa
    49681 cagaataagt ttcggtatac agtcttttaa cgacaggata ttgatgtata tgggaagaat
    49741 ccacaacagt ctgaccgcca ttaacaatat acacgaatgt aaagaagcgg ggtttgataa
    49801 tataagtgcg gatatgatat tcggatatcc caagcagaca ttggaacaat atgaggattc
    49861 cttaaatgta cttaaaagcc tggaaatacc gcatgtatcc tgttacagtc tttccataga
    49921 accgggtact ttactggatg aaatggttaa aaagaatgtt atgccaatgc cggacgaaga
    49981 tctggacagg gcaatgtata acttaagtat agaaagtctt aaaaaggcta acttacagca
    50041 atatgaaata tccaatttcg ccgtccccgg ttatgagtca agacataaca taggttactg
    50101 gaaccgtgac gaatacttgg gattcggttt atccgcacat tctttgtata atgagacccg
    50161 atacagtaat acagaggatt tacatcagta catatcttta ttgaataagt atgaaaagcc
    50221 cgttgtacac accgatacac ttacaaaaga ggagattata accgagtaca ttattcttaa
    50281 actaagaatg aacagcggat tggatattgc tcatttccaa gacaaattcg acctgaattt
    50341 tgaggaaaag tacggtgata agttaaaaac gcttgtaaat acaggcttgc tgtataagca
    50401 atcggacagt tataaactga caaggtcggg aatggatgtg gcaaataagg tatttgtgga
    50461 gtttgtgtaa atgtttgagg aaacagggga ataaagatta tttacacttg cccgtatgga
    50521 accgactttg gtatatttat gtagatacaa ggcactaagc catatataat atgtaaccgg
    50581 gagcgcaaac ttatgcatat tactataacg gggatgctgg gaagcggtaa atccactgta
    50641 tgcagaatca tatcggaaca gacaggttat gaggtatatt ccaccggaaa aatacaaaga
    50701 caggtagctg cacaaaaagg tatctccact ttggaactta ataagatgat gtcggataat
    50761 accgaactgg ataatattat agacaatgag accgtccgta tctccgatgc ggttaaagac
    50821 aggataatac tgttcgattc ccgtatggca tggttctttg ttaagcagtc atataaagta
    50881 tttcttaccg tagatcctct tgtagcggct caacgtgtag ccaaagcgga cagaggagga
    50941 gtggaagatt ataccgatgt aaacgaagca aaacaaaaac ttttggaaag agctgcagcc
    51001 gaacatgaac gttttatcaa aatctacggt gccgattact gggattacaa taattatgat
    51061 cttgtattgg atgccacatg gcatgatccc cacactttag caagtatagt tatgggacaa
    51121 ttcgccatgt ttacggaaag cgatgagcag caatccgcca tacttatatc tcccaaatcg
    51181 ctttatcccg tatttatgga aggcaagaag caacccgaca agcaatcgtg gaatgcggca
    51241 aatgtacgga tagttgccga taatcactac aattatgtta ttaaagggca tgccgtagtg
    51301 ttaagagcat tggagaacag aacggagttt atttcggttc tgtataataa ggatattaaa
    51361 accgacaatt tggttgtaca caaagattct ttaagtcttt tcgagaaata cggagagttc
    51421 aagtataaaa gtctgccgga taatgtgatt taatcacata tatcttcttt ttaacaaaat
    51481 agatgttgac aaaaatatga caggatggta tctttgagta gagttagcac tctatgacgt
    51541 tgagtgctaa aaagggagaa aaatggataa aagagcagcc aacatactgt tggttataat
    51601 cgaagaatat gttaacagcg cggagccggt aggatcaaaa agtattaccg aaaaatatcc
    51661 tctgggtgtt tccagcgcta ccgtaaggaa cgaaatgcac aaactcgaag aagaggggta
    51721 cattacttcc ttgcacactt cttcggggcg tatacctacc gataaaggct acagatttta
    51781 tgtcgactac cttaccgagc atatccgtca aagagagatt gaggaaaaga tatataaact
    51841 gcttaaaaaa gtggaaatag aagaaactct gggctatata ctggaaagac tgttcttggg
    51901 taatccgtat acttccttgg gagtgctcaa aagaaatgtc agccgcatag atacattcca
    51961 tatactgtcg gtggccaaag atacttatct tgtactggtt atattcagaa acggggtagc
    52021 ggcacacaga cttatcaaag aacagcttag cgacaaagtg caggtaaaca ttttatccga
    52081 gtatctcaac gaaaagctgt acggtatgac attgagagac attcagacaa ccgattttgc
    52141 ttttatagat acattcgacc gcgacaataa agctttcttt atccgggtgg tatcggaagt
    52201 aatggatata ctgcatcagt atgaggataa cgagttcttc ctcaaaggaa aggaaaacct
    52261 tttgtctttc ccggatttca aggaactgtc ggaagtaaag agtatctttg atgctttcag
    52321 cagggaaatg gaactttccg aactgctgtt atgtcctacc gacgatgctt tttccgtcag
    52381 tataggaaat gagataaaaa atgagaattt caacaaactt tccatagcca agcatacata
    52441 tgatttgggg gcggaaggga aaataacgtt tgcggttgcg ggaccgacaa gaatggatta
    52501 caggagagta atgaactctc tcaattacat ggaaaccata ttgggagata tcatatatga
    52561 caaaaatgac aagaaggagg tacagtagtg gcaaagaaaa aggaagaaaa agacgtgaat
    52621 atacagcagg acggagcgga agtttccgat gagagcaata ccggtaatga agaagtaact
    52681 tcgtgtgaga ccgatattga aaaagaaaac cgtgaactga ccgacagtta taaacgtctt
    52741 gcagcggaat atgataatta caggaagaga accgcaaaag aaaagcagga catctatgat
    52801 atatgtgttg cggacataac ggagaaattc ttacctgtat tggataacct tgatttggca
    52861 ttggcaaact gcgattcgtc cgatgacaat gtcgtaaagg gaatagaatt gatacataag
    52921 caattcctgg aaatcttggc aaaactggat gtttttgaga ttgattgtct taacaaaccg
    52981 tttgatccca acattcacga tgcggtaatg gtaacggaag atccggatat tcagccgggg
    53041 accgttgtag aggttttcaa aaaagggtat atttacaaag accgtgttgt tcgtcacagc
    53101 gtggtaaagg tagcacaata ggaaagaaat aatttaaatg aaaataaggt caaaaaaagt
    53161 caaagacaaa taaggaggac taaaatggca aaagtaatag gaatagattt aggaacaaca
    53221 aactcatgtg tagcagtaat agaaggcggt gaaccggtag taataaccaa tgccgaagga
    53281 gcaaggacaa caccgtcggt tgtggcattc tccaaaacgg gagagagact ggtcggacag
    53341 gttgcaaaaa gacaggcaat aaccaatcct gacaggacaa tagtatccat taaaagagaa
    53401 atgggttcgg ataagaggat taatatagac aataagaaat attcaccgca ggaaatttcc
    53461 gcaatgattt tgcagaaagt gcgtgccgat gcggaagcat atctcggaga aaaagtaact
    53521 caggcggtta ttaccgttcc ggcatatttc tccgactcgc agagacaggc cacaaaggat
    53581 gcggggaaaa ttgccggtct ggatgtattg cgtatcataa atgagcctac cgctgccgct
    53641 ttggcatacg gaatggataa ggatcaggac cagaagatac tggtgtttga cctcggcggc
    53701 ggaaccttcg acgtatccat attggatata tccgacggag ttttcgaggt tcttgcaacc
    53761 aacggtaata cgcgtctggg cggagatgat tttgataata aagttattga ttatcttgcc
    53821 gaaactttca agaatgataa cggtatagat ttacgaaatg ataagatggc tttgcaaaga
    53881 cttaaggaag cagcggaaaa agcgaagata gagctgtcat cggtgttaag caccaatgtc
    53941 aatctgccgt ttataacagc ggacgctaca ggacccaagc atttggatat aacgctgtcg
    54001 agagcaaagt tcgatgaact gacagcggat ctggtacaaa aaaccatgga tcccttaaga
    54061 aaagcaatga atgatgcagg gttaaagcct gcggacatca acaaggttct tttagtgggc
    54121 ggttccacca gaatacctgc cgttcaggat gcggttaaga agtttatagg aaaagaaccc
    54181 tttaagggaa taaatcccga cgaatgcgtg gcaatgggtg cagccataca agccggcgta
    54241 ctcacaggag atgtaaaaga tttacttctt ctggatgtta ctccgttatc attgggtatt
    54301 gagaccttgg gcggcgtatg cacaagactc attgaaagaa ataccacgat acctaccaaa
    54361 aagtcacaga tattctccac cgctgcagac ggacagacgg ctgtggacat tcatgtgctt
    54421 cagggagaaa gggaaatggc ccaatacaac aagacattgg gtaaattcca gcttaccggt
    54481 atagctccgg ctcccagagg aatacctcag attgaagtta ccttcgatat tgatgctaac
    54541 ggtattgtta acgtttcggc caaagatctg ggaacgggaa atgaacagaa agtaaccatc
    54601 accgcttcca caaacttaag cgaagatgag atacaaaaag ccgtaaatga tgcaaaagct
    54661 catgagaagg aagacaaact gaagaaagaa gagattgatg caaggaataa tgccgactcg
    54721 cttatctatc agacagagaa acttttgaaa gataatgccg ataaagtcga tccgacagaa
    54781 aaggcaaagg ttgaagagca gattgaaaac cttaagagtg cattgaaagg caccaatgtg
    54841 gaacagataa aaaccgctac cgatgattta cagcagtcgg tctacaaact ttcggaagga
    54901 atgtacaagc aggcgggtcc cgatatgggg caggcaggtc ctgatgccga ttcccatacg
    54961 acggatgacc atacaaaaga tgacaacgta tatgatgccg actatgaagt ggttgatgat
    55021 gacaagtaag tcggtataat atataatggt tcggtcgctt aaggcggcga tgaaaatcgc
    55081 cgcttaagtt aataaataaa acaaaggacg ttacttgtgg cggaaaaaag agattattac
    55141 gaagttttgg gcgttgaccg caacgctacc gatgaagaat taaaaaaggc atacaggata
    55201 ttggcaaaaa aataccatcc tgacgttaat gatacacccg aagcggaaac acttttcaag
    55261 gagataggtg aagcatacag tgtgttaagc gatcccgata agagaagaca gtatgaccgg
    55321 ttcgggtttg acggcttaaa cggagccttt tcttcgggtt ttgcgggagg ttttaatccc
    55381 tttgacattt ttaacgattt gttcggcgga ggcttcggca gcggtttctc atcgggtttt
    55441 tccagaagaa atgcccctat gagaggcagg gatattaacc aatctataaa gatttctttt
    55501 gaagaggcgg ctttcggtac caaaaagacc attaccgtac aaagaagcga gtcctgcgag
    55561 cagtgtgaag caaccggagc caaagacgga aaagccatca agacctgccc tcagtgcaag
    55621 ggcacggggg agatgcgtgt tcagcagaat acactgttcg gacagatgat aaccgccacc
    55681 acatgtaaca aatgtaacgg aaaaggcaca gttatcgagg aactatgcga gcactgcaaa
    55741 ggtgcgggta ctattcgggt aacaaagaag attgaggtta acatacctaa gggaattgat
    55801 aataaccaga ttatctcatt aagaggacag ggtgatgccg gtaaaaacgg aggaccggaa
    55861 ggagatttgc gtgtgcatat tacagttaca cctcatgaga tatttacaag agaaaacact
    55921 acggtgtact gcacaatacc cgtaacctat gcacaagcgg tattaggtgc ggaaataacc
    55981 gttcccacat tggacggaga cattaaatac aacataccgg cgggtaccca gaccggtaca
    56041 acctttactt tgaaaaacag aggaatacat tttctcaaca aagaatcaag aggagatcag
    56101 atatttgaag tatatgttga tgtacctaaa aagctgaaca gggagcagaa aaaggcactg
    56161 gaagcttatg cggctctttt tggtgaatca accgataaag acaaaaagaa aaagagactt
    56221 ttttgatgga atggataaaa gttaagatat ttacggataa ggacaatcag gaagagttac
    56281 agtggttcct tataaatatg ggaattaacg gctgtacagt ggaagacagt gcggatttta
    56341 acgatttcct taaggataaa tcctttaact gggattacat agacaatagt ttaatgaagt
    56401 ggaaggatat aaggagcagc ataaccgtct atcttgccga taatgagcag ggcaatatgc
    56461 aacttgactg tataaagaat gcatataccg atattgaaac ggaaaagata tccgatcaag
    56521 actgggcaaa taactggaag caatatttta aacccataga aataggcaag cgtctggtga
    56581 taagaccttc ctgggagcaa tatgacaata ccgataacag aagagttgta acattggatc
    56641 ccggaaacag tttcgggtcg ggtactcatg agaccacaaa actgtgtctt atgcagtcgg
    56701 aagctttggt taaagagggt ttcgctgttt tggatgcggg ctgcggttcg ggaatcttag
    56761 gcataagcgc attggtcttg ggtgcaaaaa gttgtgtgtt tttcgacata gatcaggcaa
    56821 gtgccgatac taccatggca aacgcaattg caaacgatgt acaggataaa tcgctggttt
    56881 actgcactga tattcttgaa gacatggaat catattcttt acttttagca aaaggtcctt
    56941 acgatatggt gtttgccaac atagtgcccg acgttatagc cgcaatgctt ccttttttga
    57001 gtaaagcggt taaatcggac ggttacctta tctgttcggg aattattaaa gaaagagaaa
    57061 aagaaaccgc tgataacatg agaaagtacg gatttagcag tatagagtgt aatgaactaa
    57121 acggctgggt tgcgcttacg gggagaaaag atgcatagat tttttgttca atcaaaattg
    57181 aataataata tgacggttcg catagaaggc gacgattatc agcatctcaa aaactcttta
    57241 cgcataagaa cgggagaatt aatagagata tgcgacgggt gcaacaatac atttattgca
    57301 tctgtcggta atcttaacaa aaattactgt gaagccgtct tgcaggagag aataccttta
    57361 agtaacgagc ccggttgtaa aataaccctt tatcccgccg tattgaaagg cgaccgcttt
    57421 gaaagcatga tacaaaagtg cgtagaaggg ggagtatatg ctgttaaacc tgtaattaca
    57481 agtaacacga tagtggatat ttcttctgaa aatgccgtaa aaaaacataa aagatggtcc
    57541 agggtatgca agcttgcctg tatgcagagt aaaagggata ttttagtgaa tattgataag
    57601 ccggtaaagc tgtttgatgc ggtaatgtgt ttacaatcgg gtaataattt aaataataat
    57661 gatagtaaga aagaaagtaa gagatataga aaggaaatat cctttgtctg ccatgaagcg
    57721 gaagaggagc attccataga tgcgatactg gaaaaagctt tcggtgagaa agtggagaat
    57781 ataaacatat ttatcggtcc ggagggcggt ttttcggaac aggagattga aaaattgaat
    57841 gaatacggga ttctttcggt tcatatggga aaaaggatat taagagccga taccgcggca
    57901 tacagtgcgg tattctatac ggccatgaga tatgagctta accgtgtgaa tcaataagtg
    57961 attgttgtag aatgaagaaa aagtaagtag ttatttggag gtgcaaaatg aaagaaaaag
    58021 gaaacatatc cattaatacg gagaacattc tccctatcat taagaaatgg ctttattccg
    58081 ataaagacat atttttgaga gaagtggtat ctaacgccat tgatgccgtt acaaaacata
    58141 agaaagtatc atcgttaggt gagagcgagc aaccggagaa atacagaatt gatgtcaaag
    58201 tcaataagaa aaacaagacc gttaccgtat cggacaacgg tataggaatg acttttgacg
    58261 aggtaaagca gtatataaat cagattgcat tttccggtgc cattgatttc ttcgagaaat
    58321 acaaggataa gaccgatgca accgatcaga ttatagggca tttcggttta ggattctatt
    58381 cggtgtttat ggtatcggaa tatgtggaaa tacagaccaa atcatatatt gaatcggagc
    58441 agccggttta ctggaaatcc gacggaacca gcggatacga gatgagtgaa ggcacaaggc
    58501 agacccacgg gaccgacatt ataatgcata taaactccga taaggagaat aaggatctgg
    58561 ttaatgtgtg gaagatgcgg gaaattttag ataagtattg taaattcctg cctgtggaga
    58621 tttatctttc cgaagagggt gcaaagaaag ataaagacgg taaggatgct tcggaagaaa
    58681 aacccgtcaa caacacttca cctctgtggc tgaaagacgc caaagactgt acggatgagg
    58741 aatataaggc attttaccgg gaagtcttcc gtgattttaa agagcccctg ttctggattc
    58801 atctgaaaat ggactatccg gtcaatctcc gcggcatact gtatttccct aaacttacca
    58861 atgaatacaa tacattggaa ggtaaaatac aactttattg caaccaggta tatgtagcgg
    58921 acaacataaa ggaggtcata cctgagtatc tgttgctgtt aaaaggcgtt atagattgtc
    58981 ccgatttgcc gttaaacgtt tccagaagct ttttacagaa tgacggttat tcgggaaaga
    59041 tagccggcca tatatccaaa aaggtatcgg ataaactgaa acagttgtat aagagcgatg
    59101 cggagcaata ctacaagtac tgggatgata tcaacatttt tgtcaagtac ggatgcttaa
    59161 aggaagagaa gttctacgat aaggtaaagg atatcatcgt atacaaggat atatacgaca
    59221 agcatcatac tttggagcaa tataagaaga ttaatgaaga gaaaaagcag aaggatgtaa
    59281 tatattatgt ctccgatatg caaaagcagg ctcagtacat caatatattc aaagaacatc
    59341 aacaaaacgc ggttatactg gacaatatac tggattcgca tttccttacg tttatagaat
    59401 ccaataatcc cggaacctct tttatgagta tagacagcgg cattaaggat gtattggcgg
    59461 aagaaggagc gaccgaagga tttgacgata ttaaaaagtt cttcttggag cttctgaagg
    59521 ataaaaaggt cagtgtggaa gtagaggcat taaaatcttc cgatgtaagt gcagttataa
    59581 tatccgatga atatgaaagc agaatgaaga acatgcagac aatgtacggt atggaattcc
    59641 cttcgagcaa cacccttgtg cttaacacac ggcatccggt tataagtaag ctggcaacta
    59701 tggaagatga gcaaaagaag agcgatatag ccatatacat ttatcaactg gcaatgcttt
    59761 ccgcaggtaa attggaaaaa gaagacatga cggattttct taaaagaaat aacgaaatgc
    59821 ttaacaagag tacggaatag tgtagtatta tataatcaaa taaatgcgga gcgtacggac
    59881 tgtgggaaat ataagaagaa tatatattga gcggaaaagc ggatttgatg cggaagcgaa
    59941 aggtcttttg gaccaatgtg taaatttact gggaataaag aaactggagc atatccgtat
    60001 tctcaacaga tacgatatac agggaatgga tgacagcata tatgccgtag ctaaatacgg
    60061 catttttgcc gaaatacaga ccgatacggt atatgaagaa gaatttgatg caaaggatgc
    60121 tgcatatgtc tttgcatatg agtttcttcc cggacaatat gatcagagag ccgattctac
    60181 cgaacagtgt atacagctca tatcccatac aagaccaata gtaaagaccg ctaaggtggt
    60241 atgcctgtac ggaaatgtga aaaatgaaga tatagaagca ataaagaagt atcttatcaa
    60301 tcccgtagag tgccgtatta taagtataaa aaaacctgac aatcttaccg aacacttcga
    60361 ggtgccgcaa agtgtggata cagtaaccgg ttttataact ttccattcat ccgagttgaa
    60421 aacacttaag caacaatatt catttgccat gagcgatgag gatttattgt ttgtacagca
    60481 atatttccga tcggtacaca gagatcccac cattaccgaa ctgaaagtat tggataccta
    60541 ttggtcggat cactgccggc ataccacatt caataccgtt ttgtccgata taacgataga
    60601 aaaagacaaa tataacgaga catgtgaaaa tacttataaa agatatttag agataaggaa
    60661 gcaactgtat aaaggtaaag aaaaagacat aagtcttatg gatatcgcaa ccataggtgc
    60721 cagatacctt aaaagtaaag gactgttgga tgatttggaa gataccaaag aaaacaatgc
    60781 ctgttcaata gtggtagagg ctaaagtgaa tgagtctttg gaaaaatggc tggttatgtt
    60841 taaaaacgag acacataatc atcccaccga aatagagcct ttcggcggtg ccgcaacctg
    60901 tcttggcggt gcaataagag atccgctgtc gggaagaagc tatgtgtatc aggctatgag
    60961 agtaaccgga gcgggaaacc cttttactaa aatagaagac acaattcccg gaaaactgcc
    61021 tcagattaag attattcggg aggcggcaaa cggctacagt tcctacggta accagatagg
    61081 acttgccaca ggcttggtgg atgagattta tcatgacagt tatgtggcaa aaagaatgga
    61141 agtgggagcg cttatcggcg cggctcctct gaagaatgta aaaaggtgtg atccggcaga
    61201 cggtgatgtg gtattgctta taggcggaag aacgggcaga gacggtatag gcggtgctac
    61261 cggttcatcc aaaggacaca ccgagcaatc gttggaaagt tgcggggcgg aagttcagaa
    61321 gggtaatgcc acgatagaga gaaaaataca gcgcctgttc cgtaatcgga aaatgagtct
    61381 tttgattaaa aaatgcaatg acttcggagc gggcggtgta tcggtggcta taggagagtt
    61441 ggccgacagt ctggatatat atctcgataa agtacctaag aaatatgaag gtcttgacgg
    61501 taccgaactt gccatttccg aatcgcagga gcgtatggcc gttgtggtgg ataaaaaaga
    61561 tgttgataaa gcattgaaac tggccgaaga ggagaatctg gaagctacac cggttgcaaa
    61621 agtaaccgat agcggtcgtt taagaatgtt ctggaacgga cacaccatag tggacataga
    61681 aagaagcttt ttagatacca acggtgtgaa acaatatgct aaggcttata ttgccgggaa
    61741 agacgaaaac aacaatccgt tttatctgaa aacccgtgga gacaaatcat ataaagagcg
    61801 gttgtttgaa tcggtaaccg atctgaatgc atgttctaaa aaaggtctga ttgaaaaatt
    61861 tgacagtacg ataggttccg caaccgttct catgcctttg ggcggaaagt atcagataac
    61921 gccggcggaa gtcatggcgg caaaacttcc cgtattgaac ggtaagacaa attattgcac
    61981 attgatgtcc tacggatttt tcccgcagat ttttgaatgg tcctgcttta ccggtgcctg
    62041 ttattcggta ctttcatcgg ttgcgaaaat tgtggcagcc ggaggtgact ataccaatat
    62101 acgtttgaca ttccaggagt atttcgagag tttaaggaaa gatcccgtaa gatggggaaa
    62161 acctttacag gcggttttag gagcactgca tgctcagttg gagctcaaga tgccttcaat
    62221 aggcggaaaa gactccatgt ccggttcttt tatggatatg gatgtggttc ccacccttat
    62281 aagctttgcg gtcagtcata ccgatgccga aaacgtgtta tcaccggaat tcaagaaggc
    62341 gggtaatacg gttattcgtt tcagtcacag acaaaaagcc gatagcactc ccgattttgc
    62401 cgactcactg aagatttttg ccgctttcaa ggaattgaac caaaagaaag cgatattgtc
    62461 cgcaagtacg gtaaaacaca aaggagcggc tgaagcgtta ttaaagagct gcttcggtaa
    62521 tatgataggt tttgaattat cggccgatgt gaacacctta tttacccccg gctattgtga
    62581 tatcatagcg gagattgact cggataaaat agaggatata gaaatatcat atatttttga
    62641 taagtataaa gaagttgata tctcggttgt aggaaagaca attgaagata agcaatttgt
    62701 catgggtaac ctttccgttg ctcttagtga attggttgag gcatataccg ataaatatga
    62761 acgggttttc cccaccaaag caaaaacaaa tactccgaag ataaacaata ctttatatga
    62821 cggtaagagt attcattaca gtaagaataa aattgcaacc ccctatgtat tcataccggt
    62881 atttcccgga accaattgcg aatatgatac aaaaagagct tttgaagatc atggtgcggt
    62941 atgtgacttt tccgtgttca gaaatcttac acctgctcat attgacgact cggtaaagga
    63001 ttttgttaaa aagattaata aggccaatat tatcgccata ccgggcggct tttcttcggg
    63061 tgatgagccg gacggttcgg gcaagttcat agcgacggta ttccgcaacc cgtatataag
    63121 agaggcggtt tacgatatgc tgtacaacag agacggattg atgctgggca tctgtaacgg
    63181 atttcaagcg ctcataaagt taggactggt tccgtatggt aagatggttg aacttaaaga
    63241 aaacagccct acattgactt ttaacagtat tggaaggcat gtatcctgta tggttaacac
    63301 aaaagtggtc tccaaactgt ctccgtggtt catgtgtgcc gacctcggtg aagtatatac
    63361 aataccggta agtcacgggg aaggacggtt tatctgtaca aaggaagaat atttaaatct
    63421 ttcaaagaac ggacagattg caacacagta tacggatgat tacaacaatc cttcgtatga
    63481 tatacagtac aatcccaacg gctcctatta tgccgttgag ggaataacca gtcccgacgg
    63541 caggatattg ggtaagatgg gacattccga aagaataggg cagtatattt gcaagaatat
    63601 acctggcaac aaagatcaga tgctgtttgc atcgggtgtc aagtatttca gataaggtat
    63661 cggagggtaa aatgatttca aaaacagtta ttaagaaagt attggaattc gataatccgg
    63721 ggaggatagg atatgatttc cttgacggtt ctttaaggga ttttaaattt gtagagctta
    63781 atatccccgt aaaagagcac aataaatggc agaagcccgc atattttgcc gatatgtatc
    63841 ccgaatataa ggattttgac ggcactctcc gtctggatga atacggtaac ttatggggac
    63901 gtatggctca tgataccacg ggcggaggag aagtcctgga aggtgctttg aaatcctggg
    63961 aggatctgga ttcatataag ttgccggata ttgatgatga gaaaagattt tttaatgcac
    64021 acttaagcgt ggaaccgtat aaagaacgct ttatactggg tcctataccg ggctgtacct
    64081 tcgctattat gagaaatatc cgcaaaatgg aatactttct ggaagatctg gttctggagg
    64141 aggataatgt tcttaaactt caggatatgg ttataaccaa gttggtaaac atggtggaca
    64201 tgttcggagc catgaagtgt gttgacggaa ttttcttctg tgaagactgg ggcattcagg
    64261 acagactgct ggtatcccct gcaatgtgga gagaaatttt taaaccggct tttgttaaat
    64321 tatgtaccgc tgcgcgcagg aataacttaa aggttcttat gcattcctgc ggttatattt
    64381 atgaaatatt agacgatctt ttagaagtgg gaattgatgc ttttcagttg gatcaaccca
    64441 cattgatggg aatagaacgg ttggcggaaa aaataggggg gaaggcaacg ctgttttcac
    64501 cggtagatat acaggcaata cttcctacag gaaacaggca acttatacga gacgcagcca
    64561 ctcagatggt agacctcttc tataaaaacg gaggcttgat tgccaaagat tacggtgact
    64621 acaatacctt acgcattaaa gacgaatggg cgggatgggc tcgggagaga ttttttgaga
    64681 taggagggtt tgatcccgga aagaagtaga attcccgatt taatgatttt atggtatatt
    64741 ataaaagatg aaattttcgg agcggataat tgactgatac ggataacgat aagatagaaa
    64801 cagaagagaa taccgaattg cctttagaac aggttgacga taccgccaat gagcaggatt
    64861 ttgaccaagg tcgggagcag ggtatagaaa agaagcagag taagatactt acttttatcg
    64921 acggtatctg gaaatttttc acaaaagaac aagcggaaaa gcgaagagaa agaaaaaaga
    64981 ataaaaagat tagaatagtt gtatataaac ccaagtctca attcaggaag tttatatcgt
    65041 tgatattgtg gttgggattt ctgtttacat tgggatttgt tttctacaaa ggatatactt
    65101 tcacgaacac ccttgtaaaa gaagttaatg tatttgatgc caaagctgca ataggcacca
    65161 tagaagagaa gaaccggaag gtcgtaaaca tacccaaggg tgcaaatacg aaagaaatag
    65221 ctaaaatact tgtcaatgaa ggaattatca taaacgatac ctatttctgg gtttattcgt
    65281 tgttaatggg taatgacggc aactataaat ccggcaatca catttttgat gcttcggtca
    65341 cttatgattc gggaagtata gatgttgtta cggctcaggg atatgaaatg cttatttaca
    65401 ttctctcttc ggacagtgtt cccaatccct ccaagaagat tttctttacc gaaggtctta
    65461 caatggcaca gacagtggag aaatttacgg aaaacgacat acgcacaaag gaagagttct
    65521 atgaggtctg caataattat gagttcgaat atgattttgt aaagcagata cctaaggatt
    65581 ccaggcgtac caaccgtctg gaaggctatt tattccccga cacatatatt ttcgatgtta
    65641 ccgtcaatgc cgaagaggat attaaaaaga tgctggataa cttcaacagc aaatttaatg
    65701 agacctacag agcaaaagca caagctttag gatatacaat agatgaagtg gtgattatag
    65761 catcgctgct ggaaaaagaa gcatctacaa tggatgaaag ggcaatgata gcgggtgtgc
    65821 tttacaacag gatgaattct aaagagacat atctcaatta tcttcaggtg gatgcaacga
    65881 tacagtatat ttacctcaat gagacgggaa aacttaagga gcaattgacc aacgaagatc
    65941 atgagataat gaacagttac aacacctaca tgtacaaagg actgcctccg ggaccgatat
    66001 gcaatccttc tttagccagt atagcggccg ctttatatcc cgaaactcat aattacttct
    66061 attatgtggc gaaaaatgac ggaaccgatg ctcatgcata tgccaccact cttgaacagc
    66121 ataactatta cgttaagtta tatcagaaat agccatggaa aaacgcttca acatagtcaa
    66181 taacgatgtg gaaaaatata tacaagacac attagcaccg cttagggaca atctgcaagt
    66241 gatgtacagg aacgctttaa aaaattgtgt tccggttgtg cgattggaag tcttaagagt
    66301 actggaaacc taccttattc ttaaaaaacc gtattcatta cttgaactgg gcaccgcaac
    66361 tggttttaca agtattttct tttccgaata tctcaaagat aacggttata ttgataccgt
    66421 ggaaaatgat ttggaaatgg taaaaagagc acgagagaat ataaaggaga tggcacccga
    66481 caagaatata aatgttatat atgcggaagc gtgcgactac ttaaaaaaca tcaataaaaa
    66541 gtatgatatt atattcatag atgcggcaaa agggcattat gaagaatatt ttgatatatg
    66601 ttcaaacctt gtcaatgata acggcataat cttttccgat aatgtcttat acaggggaat
    66661 ggttgccaaa ggcgataaaa tacctcacag acagaaaaca ctggtggaaa gattgcatag
    66721 atatctggat aagattacaa aggatgaaag gttttgcaca tcgatattat ccgtaggaga
    66781 cggattatcg atttcggtaa agaaatgaga gcgatagaac ttctggcacc ggccggaaat
    66841 atggaaaaat taagaattgc atatgcatac ggagcggatg cggtatatct gggcttaaat
    66901 aagttcagtt tacgcgataa agcggataat ttcagttatg aggaactgga acatgctgta
    66961 gcctatgcaa gagccctcgg aaagagaata tatgtcacgg ttaatatact gccgaagaat
    67021 aaggatttgg aggagattaa aaagacattt gaccgattgt cagatatttt acccgatggt
    67081 atcataattg ccgacatagg gcttattgag ttggcaaaga aacatgccaa aaacattgat
    67141 atacatataa gtacacaagc caacatatta aataaagaaa gtgcaaaagc attttgcagg
    67201 ttaggtgcca aacgccttat cttaggcaga gaactgtcgc tttccgatgt ggcccaaata
    67261 aaggatgccg taggagcaga gcatgagatt gagatattca ttcacggagc catgtgtata
    67321 tcctattctg gaagatgcct tttaagttca tatatgatag gaagagacgc aaatcagggg
    67381 gattgcgctc aaccgtgcag atggaaatat gctctgatgg aagaaaagcg tcccggtcaa
    67441 tattttccca tatatgaaga tgatttaggt tcatatattc tcaattccga cgatttgaac
    67501 atgataaatc atattcccga acttttagag tgtaaagccg acagttttaa gattgaagga
    67561 cgtatgaaga gtgcctttta tgtggctacc gttaccaaag cataccgtac ggcatttgat
    67621 gattgtatca gggataagca aaagtattta tcttcactgg aggaatacaa cgagattgtc
    67681 tccatggcaa gccacagacc gttcacaacg gcatataatc tggggtatga ggactttgta
    67741 aataaaacaa gagaatccat gacatactca aattccaact atatcaggga gtatacctat
    67801 tgcgggatag tggaggacta taacaaagag cttggtcttg caagagttca tgaactgaac
    67861 ccggtatata ttgacgatac ggttattctg atgtctccgc atataaacac ttttgaacaa
    67921 aaagtaataa aactttatga tgaagatatg cagccgatag ataaagcaca tcatccggca
    67981 atgaaatatt atcttaaaac agatataccg gtggaaaaag ataccattat tataaaaaga
    68041 ggtggacaat gactgacata ataggtttta tcggagcggg taatatgggt tccgcactta
    68101 taaaaggcat taaggcaagt aaagcaaaga tatttatata cgagcagcag agaggaaagg
    68161 ccgattatct tatcgatgct tcgaccaaac ttgttaaaag tgtcgaggag ttgcttaaaa
    68221 agtgcaatat agtattttta tgtgttaaac ccgacgggat ggcggaattg ctggagcaga
    68281 ttaaagcata taaagcggta aaagacatac tgttcataac catagcggcg gggaaaacaa
    68341 tggagtttta cgaaaatata ataaaagaag gcaggtttat acgggtaatg cccaatatgc
    68401 ctatggcaat aggcagcgga atggctacga tatacaaggg aaacaatgca acaaaagccg
    68461 atttgttgaa agcggtgatg taccttaaat atgtcggaga aacacttgtg gtaaaagagg
    68521 agtttttgat gaatataacc acggcagttg ccggatcggg acctgctttc gttttcctgt
    68581 ttataaaatc cctcatggat acagcggtaa agaacggcat ttcaccggaa gatgcaaagc
    68641 tgttggcatg tcagactgtt gaaggttcgg caaaatatgt aatgcaacag gatgccgaca
    68701 tggaaaccct tatacaaagt atatgttctc ccaacggtac aacggttgaa ggcataaaag
    68761 tgcttaaatc aaagaatttt gaaaagactg tagaagcagc ggtaattgcg gcaaaaaaac
    68821 gctcaataga tatgagcgga gacaaaaaag agaagataaa cggtaaaagt gtaagaattt
    68881 ataccgacgg tgcttgtctt tacaaccccg gtccgggcgg ttatgccgct attcttctgt
    68941 acggaaataa ggaaaaagag atatcggggt ataaagaaga tacaaccaac aatgaaatgg
    69001 agcttacagc cgcattggag ggactggcac agcttaaaaa gagttgtgat gttacggttt
    69061 attccgattc cgcatacctt atcaatgctt ttaatcaggg ctggatagat tcctggaaga
    69121 gtaacaactg gacaagagga aagaacgaag aaataaagaa tcttgagctg tggcaatctc
    69181 tttatgaaat gaatcaaaaa cataacataa agtgggttaa agttaaaggt cacagtgata
    69241 atgaatataa caacagatgt gacaggcttg caaataaagc aattaaggat aatcagaata
    69301 agtgagcaag aaatatcttg aatggaacaa ttcgcccgag gcgatatatc tgtattcgct
    69361 gttaaagacg gtatttgaga gaattttacc aagaaaaaga aagacccctt caaaaaaaca
    69421 actgattaag atgtcttccg atttaagtta ttcttcaaag cagcgattga ccaaaaataa
    69481 tgctgaaatg tatctgaaaa ggaacagaaa tattcttaat accgcattac ctcataggaa
    69541 taaattaacg ataagtgata actgttttga tgagatatac aaaaaaatgg cgatatgtga
    69601 gttgtatctg tttaacaggt acaacaaaga aaacggtcat atacccgata tgtttacact
    69661 gtttgaggat accggtaatg tatttcagtt aaaaaacaat accgttcttt cgatatatat
    69721 ggaaagatat cttaagaaaa tggctaaaga acagattctc aaacttattc cccaaaatcc
    69781 caaagatgag ttttgcgaaa caagaaaaat gaaaagatac tttgttattc attccggact
    69841 gaccaataca ggtaagacat atcatgccct aaacgcgtta aaagagggaa aaagcggtgc
    69901 atatctttcg ccgttgagac tgttggcact ggaagttcag gaacgtctta tgcaggacgg
    69961 tgtgttatgc agtatgctca caggggaaga agaggatata gtggaaggcg caaagcatat
    70021 atcctcaaca gtggaaaagg cggattacag tcaaaattat gatgtagcgg taattgatga
    70081 atgtcaactt attcaggacg aagcaagagg ctgctattgg acaaaagcta ttttaggcat
    70141 aaaagcacaa actattcatc tttgctgtgc cccggaagcg gaacagattc ttgtgaaaat
    70201 aattgaagac tgtgaagagg aatatgatat tatccgacac aagaggaata ccgaacttat
    70261 cttttccgaa cagccttttt ctctgaagaa tacgcaaaaa ggtgatgctt tagtggtttt
    70321 ttccagaaaa agtgtattaa gagttgcgga cgagttatcc cgttacaata aaaaagccag
    70381 cataatatac ggagcattgc cttatcagac cagaaaaaag caactcggtt tatttctgga
    70441 aggcaaaacc gatgtggtgg ttgcgaccga tgcaataggt atgggactta atcttccgat
    70501 acaaaggata gtgtttctgg aaacggaaaa gtttgacgga actgtcatca gaccgttagt
    70561 tccctgggaa gtaaagcaga tagcggggcg tgcgggaaga agaggaatat acgaaaaagg
    70621 atatgtctcc acagtaagtg atacaaatct ggttaaacac aatcttaccg ttcccaatgc
    70681 caccgttgaa tatgcgtatt taggatactc cgacatactt ttagatattg aatgtgatat
    70741 aacgcaggca ttgagagtat ggaaatccat tcctaccggc ggtttctatg ttaagcagga
    70801 tatttccagg acattgaact tatgcgatca gattagaaag atttataaag acttcagtaa
    70861 gagagaatta ctgaaactgg catcaatacc ttttgatgaa gagaacaggc aggtaatgga
    70921 gctttttaag gagtatactt tactgttttc ctactcgcaa ccgatattta aaccggtaat
    70981 aatctcctat actttggatg ctctggaaga ctactacaaa tgtctggact tgtattactc
    71041 gttttccaga agtatgggtt atactcccga tttggaatgg ctcaaagaga caaagcatat
    71101 aattgccgag aagataaata aagagttgct tttaaactca tcgggacaac atgcaaagaa
    71161 acaacggaag aaaagcaatt attagtatat aatgttaaca gaggcaattt aaaatgaaag
    71221 aaacgtcaaa acaagaaagt gataacaggg taaacaaggg aataaaagtt atatactatg
    71281 tgactctgac aataagtatg ttagtgggct tatggcattt ttttgtgcct aatatgttta
    71341 agtggtacga ttatctgcct atgcagtatg agaatcttat tgtaggaatc gactatacta
    71401 atttatgttt ttcggctttg cttttcggtg ccagtctcgt tcttatcata ctggcaaaaa
    71461 gtgcttttaa gcttaacttc gaaacaattg tattttatac atttttaacc gtagtctggg
    71521 tgttcagagc atgtcttgct gtgtttatag agccttggcc gttagaacct gtccctgcag
    71581 ctgcaatagc tcaactgatt tcatcggtta tgttggcagt ttttatggta attgtaacgg
    71641 tggttttctg gaaaaacaga aaggtacata aaaaagggta gagtaaagtc gaccattaaa
    71701 aatacttttt ttaaaaaaaa acggattctt caagagaaga aatagctgaa aagatttcta
    71761 aaactgtaag aacggttcaa agagcactca attctcttgt agagaagggt tatatcagtc
    71821 gagtaggctc taaacagaat ccggtatggg aagtgttgaa ataaggttag gtatataaat
    71881 aagaatttga tagaaaaggt gtggtgatga aaatgtataa atcaaaaact tggcagtttg
    71941 aggtttccgg tattgacggt aatgtaattc tgtttgggaa aaatatattt gaatatgaat
    72001 ggaacaatac cggtgaaagt gttttggtag aagaccccat atatcatcag gagttcaagt
    72061 ttccggtgta tactgtgaat attgatggac aatctcatag ttttgttgcg ggtgaattta
    72121 gtaactgcat atggggattc tatttgttta aatattagaa taacaaatta gaatttgttg
    72181 tacctgaact taagaaaact tctgatgtca gacaaatgct acacgtagaa aagcttgatt
    72241 tcaccgggtt ttatgaatgt aaagacagcc aaaaacagcg ttttgtttat gacttcttct
    72301 ccggggagta tgtcataata atgctaaagg gggaaaatgt tgtagtgaac agggtttcag
    72361 agcttccaca aagtaagaaa ttcgaaaaac tgaaggaatt agacagaaaa aatataaaaa
    72421 attaattcgg cttaccggat taagttttaa ccaatgatat aattactaaa gggttggtaa
    72481 aatagttggc ataaggcagc agaagtgagc cttaaagata ggtggtggat attatgaatg
    72541 acaataacaa caataatgtg ttgatatatc aagatgataa cggaatcaca aaggtatcgg
    72601 tccgtttttc cgatgaggat atctgggtta cacagcagca gctcgcggag gtatatgaca
    72661 ccacgcaaca aaatatcagc cagcatatag acggcatata caaagacggc gagcttatgc
    72721 cgggggcaac taacaggaaa ttcttgttag tttgaactta tgttgataaa tagttgaaag
    72781 gtcaaagatg acaactgacg ataaaatgct tgttcactgt gtttgggaac ataataaaaa
    72841 tgacagcttg atatattcaa gcaatgtgat tggtgctttt acccgtggtg cttcaaaaga
    72901 agaagctttg ggaaagatgg agcgcgagat tgagtcgtat tttctttgga caggggaaac
    72961 tccgccatct tccattgaaa tgataattat tcaggaaagt gtaacaaatt tatctattag
    73021 tgatgcagac tcagaggtct tatttgagac ggaaaaaatg gatttgtcaa ttgaagagta
    73081 tgaacgacta aaagcccttg tgcttaaatc ggcaaaagat tttttaagtc tatataattc
    73141 ttttcctgat aaaaatcaaa gcgtgttacc cataagaaaa actttttacg gcactgctcc
    73201 cagaactgcc tcggaaatgt atgtgcacac taaaaatgtc aacgagtatt attggggaga
    73261 aatcggatta gatgtctcaa atgatgggac aatagtagaa aatcgaattc ggggttttga
    73321 agaactagaa gcgagaggaa attttttgtc aggcaaagtt tataaaggta gttacgggga
    73381 agaatggtcg attccataag ttttaagaag atttctctgg cacgatagaa tccacgcaaa
    73441 agctatgtac aggatgagca tagcgacatt tggaacagga gttattccta atatatttaa
    73501 attcgaattg taattgcaaa taataagagt acatgagtat tcgtataccc ctgatgtcaa
    73561 acaaatgcta cactcggaaa aggttgattt tacgaggttt tatgaatgta aagacagtca
    73621 aaaagagcgt tttatttatg acttcttctc cggggagtat gtcataataa tgctaaaggc
    73681 gaaaaacgca gcaacaaaca gggtttcaga gtttccacaa aattctaaat tcaaaaatac
    73741 tgcacaatta tgatactttg taatcggtgc cataaagtga caccgattat ggcaccgatt
    73801 tcagacagca ttcgactaaa taattctaag tgacataagt gtcacttcgt ggtacaataa
    73861 tgtcactttg gaaagaggtg gtaagataat gaatctcgga aaagaaactg aaactcttga
    73921 atttaaaaaa tcgacgagtg agataaaagc tgctatgatt tccatagcgt ctatattaaa
    73981 taagcacggt atcggcacat tgtatttcgg ggtcaagcct aatggtgatg ttgttggaca
    74041 gaatgtgtcg gaatcatctt taagagatgt ttcaagagcg gtttatgaaa gcattaaacc
    74101 gcagatatat cctgcaattg atgaaattgt gatagatgga aaacaattga taaaggttga
    74161 gttcagcggt gatagcaagc catattccgc ggcgggcaga tactatttgc gaaccgcaga
    74221 tgaggacaga gaagtaagtc ccgaagaatt aaagactttt ttcgaagcaa ataaataccg
    74281 tacgaaatgg gaaaatagca aatccgacac tttatcaaaa caagttgaca agcttgcagt
    74341 aaaatcgttt tggcaaaaag cgataaacgc cggaagatta cccgaaggca ggtataccac
    74401 tccgctcatc ttaaaaagat tcggtcttgt taaagatgag cacttgacaa atgccggtga
    74461 ggttctcttt ggcaatacaa ggccagtttc tctaaaagtc ggtatttttg catcggatga
    74521 aaaattgaca attcttgata tgagattata tgaggacaat atttataatc tgttgaagaa
    74581 agcagaagat tacatattta agaatatacg atggaaagtt gaaattattg aatcggaaag
    74641 aagtgagatt cctgaaattc cgacggcagt gataagagag gttttggcaa acagttttgc
    74701 acacgcaatg tatggaagca ggacaaatca tgagatatgt attcatccga gtatgataac
    74761 tatatatagc cccggagatt atgccagtag atataagccg gaagaatata taaaaggtaa
    74821 tgtggagtct gagataagaa atgcgacaat ctccaaaatt ttgtacttga gcaaatacat
    74881 agagcaattc ggcagcggtt ttaagagaat agatagctta tgtaaagatt ccggaatcaa
    74941 gtattcttac gaaagcaaag ataatggatt caaattttta atatatcgac cgcaggttcg
    75001 aagtgacatt ttagatgtca ctttggatgt cactttgaat ggaacagaga aagcggttta
    75061 tgctatttta aaacaaaaac cggattcatc aagagaagag atagccgata agatttctaa
    75121 aactgtaaga acggttcaaa gagcgctcaa ttctcttgta gagaagggtt atatcaggcg
    75181 agtcggttct aaacagaatc ctgtatggga agtgctgaaa taaggttaat aaaaaaatag
    75241 aacttgatgg aggaagaata tatgacattt tgtggtgacg actgctgtag agaatgcagc
    75301 aagctatccg aatgcggcgg atgtgagaag tgcaacggac atccctttgg gggaagctgt
    75361 atcgcagaac gcaactcaaa ctttcaggaa ctaaagagcc ggttaattgt tgaaatcaat
    75421 catttgaaga ttgacggact tatcgttaaa gacttgtttt tactaaacgg agggtatgtt
    75481 aatctagaat atccccttac caacggaacc acggtaaaat tcttaaacaa aaatgacgtc
    75541 tatctgggaa atcaaattga aaaggaaaaa tcagaacgtt gctacggtgt agttgcgaat
    75601 gaaaatttca ttttgatttg tgaatatgga tgtaatggtt cagagccgga aatcatatta
    75661 tataaacgga gataaatttc agttttttgg atagatacaa atttagattt attgtacctg
    75721 aacttaagaa aacctctgat gtcagacaaa tgctacactt gggaaagttt gatttcaccg
    75781 ggttttatga atgtaaaaac agtcaaaaac ggcgttttat cctcgacttc gctacaggtt
    75841 ctaattactt gctgataaca tatacttctc aatatattcc gcaccgtcat tggacaaaat
    75901 aatattaccg gttacttctt taaactcaaa aagagtataa accattgtct cggtgtaatt
    75961 tgcaatgaca gtccattttg aattccgcaa gtagctctat tcctgtccga atgaatgaaa
    76021 acagcaagaa aaaatatact tgctatacaa aatcgtttat gttataatct cgtagttaag
    76081 ttttggaggc aaaaaatgag tgaagcttta aagataatat taggtatagt gcatataatt
    76141 gtgtgtctgg caacaatcat agtggtattg atgcagtcgg gcaaaagcga aggactttcg
    76201 ggagctatta caggcggagc ggaaaccttt ttcggtaagg gtaagggcag agcaattgac
    76261 aagcttcttt ccaagtatac cgtttatata gcaatattct ttgcggttac atccatcttc
    76321 ttatatctcg tactcaaata aagtgcggat acggaatatc tttatgattt ttaaaaaggc
    76381 ttataagcct tttcttttta cttttttgtt gcgatttaat gggaaatcta ctgaatatac
    76441 aatagtttta caatagtcac cgtttaagaa attgaacaga attatccgtt ggaatataat
    76501 ttaacgggag gttataaaga ctatgcttat acgattgata ggattattgg ttgctttcgg
    76561 tttatttatc ttccttatct gtcagaagaa agtactgaag tggaaaaaag cgatattggt
    76621 aattgtctca tttgttttgg tagtatttat gataattcag gtattttttc cgtttattcc
    76681 ggccaagaaa cctaccggac cttacggtgt cataaccgag acggcatttt ataagtataa
    76741 taccgatatt ccgggaatgg ctactaacgg caatgaaagg gagattcctg tcaaagtgtg
    76801 gtttccgaag gacgcacaag caaaaagtca ccctttgttt ttattctctc ccggatcttt
    76861 cggtgttgcc gaatccaatg aaactttatt tttagaactg gcatcacgag gctatgttgt
    76921 agtaagcatg aatcatccgt accactcttt cagctgtaag atgtcggacg gcaggaatat
    76981 aaatatggac ggaaagttta tacaaagtgt tatgaattcg cagggttcgg aagatttgcc
    77041 cggaacttta agttcattta aggagtggtt aaaagtacgg attgatgata tcaatacggt
    77101 acttgattgc atttcggact ttcagaatga ttatgattat gaacggtata ttgataaaga
    77161 acggacggtt ttatccggac attctttggg cggttccgcc gtattggccg taggtcgtga
    77221 aagagcgcaa gagattaaag cattggcggt tttggaggct ccgtttgcag cggatattgt
    77281 ggggattgac aaagacaggt atatctttac cgatcagccg tatccgttac ccgttttgca
    77341 tgtctattcg gatgcattgt atggaaaaat ggatgaaatt actacatatg atatgaatac
    77401 acgacttatg aaaagcgaca atcctatgta tgtgaataag catattaaag gtgtcggaca
    77461 tctgggatta accgatatga gattggtaac acccgtcatt acatatttga tggacggagg
    77521 gttggatacg cgtcctgcgg cggaaacctt atcggagcta aatgaatatg tactggattt
    77581 tttaaatata tacaataatt aaacgaattt tatatttccg attaatttac gtgattttca
    77641 ttactcttca aaatgacggt tgtactgtgc aaaatacaaa gtatcatgat ataatctcaa
    77701 gatgtggagt acttatgaga atattaggta ttgatcccgg ttttgccatt atgggatatg
    77761 gggtcataga ttatttcaaa aacaaaatcg aagtactgga atacggtgtt attaagaccc
    77821 aagcaaaaac cccgtttgat ttaagactgt tgcaaaatta cagaggtatt aatgaagtaa
    77881 ttgagaagta taaaccagag catgtagcca tggagcagtt gttttttaac accaatcaga
    77941 aaacagctat aaatgtatgt caggcaagag gtgttgctgt attggctgtt gccgagaacc
    78001 gattggagtt ttttgagtat acgcctcttc aggtaaaaca ggcactttgc ggttacggca
    78061 gagccgacaa gaatcagatt cagcagatgg taaaggtatt ggccaatctg aaagatattc
    78121 ccaagcccga tgatgcggcc gatgctttgg cggtagccat atgccatgcc aattcctaca
    78181 gggaagaaag gaagacggaa aataaatgta tgagtatata aaaggggaag tagtagctaa
    78241 aaaacatgat tatgtggtag tggacaatgc gggagtagga tacaggatat ttacaagcat
    78301 aaacaccatg tcgaaactgc agacacatga gcagactctg ctatatacat atcttcacgt
    78361 aagggaggat gtgatggtac tgtacggatt tgcctgtgaa gaagagctct ttctgttcaa
    78421 tatgctgata agtatatccg gtataggacc caaggccgct ttatccatat tatcggtact
    78481 gtcctatcag gaagtggcta ctgcggtagt atcggaagat gcggcatcgt tatccaaagc
    78541 acccggtgta ggtaaaaaga ctgcaatgag aataatactg gagcttaagg ataaattaaa
    78601 aggtctttac gctgccgaag gcgatgcggt aaaacagatt gcggtcagcg ataaatactc
    78661 cgaagcggtt aatgcactgc ttgtattggg gtataataca aagtatgcaa gagaagctct
    78721 gaataaggta tacgatgagt ccgattcgtt gcaggatata atcaagaagg ctttagcgga
    78781 gcagatataa aggagaaggg caatggagga agaaagattt gtaactccgt catttataaa
    78841 gaacgaccaa agcgaagcga gtttacggcc taaggttttt gacgagtatt tcggtcagga
    78901 aaagataaag gaaaatctta aggttttcat agaggctgcc aaattacggc gtgaacctat
    78961 tgatcatgtg cttcttgctg gacctccggg cttaggaaaa accactttag caggcatcat
    79021 agcatcggaa atgggagcta atttaagagt aaccagcggg ccggctatag aaaaagccgg
    79081 ggatcttgca gccatattaa ccaatcttga tgaaaacgat attctgttta ttgatgagat
    79141 acacagactg aatcgtactg tggaagagat tctgtatccc gcaatggagg attttgcgtt
    79201 ggatattatt atcgggaaag gtcccagtgc caaaaccata cgcctggatt taaacaagtt
    79261 tactttaata ggagctacta ccaaaacagg catgctttct tcgccgttgc gcgacagatt
    79321 cggcgtatcc tgcagactgg agatgtatac agtagaggaa ttaagcaaga tagtaaagag
    79381 aagtgccggt attttagggt tcaatatcaa cgaatacgga gctaatgaga tagcttcccg
    79441 ctccagagga acaccgagaa tagcaaacag gctcttgaga agaataaggg acttcgctca
    79501 agtggaagga gaagatgtaa ttacaagtga aattgccgat tacggactgc aaaaactgga
    79561 aattgacaaa ataggattgg ataatataga cagagctatc ctggaaacta taataaccaa
    79621 atttaacggc ggaccggtag gactggatac gcttgcggct tccgtaaacg aagaaaaaga
    79681 taccattgaa gatgtatatg aaccgtatct tcttcagttg ggcttcataa agaagacacc
    79741 gaaaggacga tgcgtaacac cacatgcata caagcatctg aaaatacctt atgaaccgaa
    79801 ataaaatact tttacatacg tgctgtgcac cttgtcttgt atatgttaat gaaaagctta
    79861 atgaaatgaa tgtggatttt gactcgtatt tctataaccc caatattcat cctaagaaag
    79921 aacatgagag aaggctttta acgctcagaa gttatacgtc acgttttaac attcctctta
    79981 ttgaagatga tgagttttta cagtatcttt gggaaaatgg tttccaatgt aaggattgtt
    80041 ataatataag gttggaaaaa acggcacgtt atgccaagaa taacggttat aaggggtttt
    80101 ccaccacgtt actggtaagc atttatcaga atcatcaact tattatcgac atagcggaaa
    80161 gtgttgccga taaatacggc atagagtttt tttatcatga ctttcgggaa ggttacagga
    80221 taggtcagaa taaagcaaag gagtacgggt tataccgtca gaaatactgc ggttgcatca
    80281 actcatataa ctcttcaaaa tataaagata agattaaatg ggattaaagg aaaaagtgta
    80341 agaaatgaga ctatttaaca agaaaaagat atatctgttt gacggttcca aaggattcat
    80401 gctgcaaaaa tacggtttgc aaaaaggaga gttggcggaa tcgtataata taacacatcc
    80461 ggatacggtc atgcgcatac ataaagaata ctgcgatgcg ggaagcgaca taattcagac
    80521 caatacgtta tgttccaaca ggcttgtatt aaagcgtcac ggtttggaag ggttacatga
    80581 caagataaac agtcagggaa ttgagcttgc aaaacaggcg attgacggaa gaaatatact
    80641 tatcgcggca tcaataggac ctataggaga gcttttacag cctttcggac cgttaacttt
    80701 cgaatatgca tacagtttgt ataaagacca gatatatgca tgtcgcaatg ccgatgtgat
    80761 taactttgaa acttttaccg atttaaaaga gatgagaatc gcttatcttg ccaacagaga
    80821 aacggtaaat ctgcctgtta tatgcaatat gacatttgag aagaataaca tgactcttat
    80881 gggacattca ccgaaggtat gtgccggtat tttgaagaaa atgggagccg atgtggtggg
    80941 agtaaactgt tccaacggac cggataaaat gggagatata cttaaagata ttgcggtatt
    81001 tgaagatttt gtctgtgtaa agcccaatgc gggagttccg agttttgtag acggaatcgc
    81061 tcattatgac cagggggaaa tagagttttg cggctattcg gacaatttgc tggatttcaa
    81121 tgtggggata ataggaggat gctgcggcac cactccggaa tatataaagg ctatgaatta
    81181 catcaaagat aaagacagaa gtattaccgt acctgttgat aagagcagat atatcttctg
    81241 tcagtacgga tatgttgata ttgatgactc ttatgaaacc gttgattttg atattattga
    81301 cggcgatata tacgacaata ttgacaatgc ttatgacata aatgaggagc cttgtgatgt
    81361 atataatgta ttttacagag gagataatgc acaatttatg tttgaactga taaatcagct
    81421 gcaggatata ctgaaaaagc cttttatctt ccatattgat gataaagtgt gtttagacag
    81481 tgctttaaga gcgtattgcg gaatagcagg cattacaggg tgcgatttca gtaaatacgg
    81541 agctgttaaa atttaatgtt gtttcactta accttgtcaa tattcggctt atactcgctg
    81601 agagtgaaag agaaaaccga tccggatttc ccgtcacttt ccacccatat atcttcattc
    81661 attcttttca gagtctcacg ggcaatggcc agtcccaagc cggaaccggc acttgaacgg
    81721 gatttgtccg atttatagaa tcgttcaaat acatattcga tatcctcttt ggatattccg
    81781 gttccgtaat cctgaacgga aacgatatat ttatcgtctt ttttgactat tgctattttt
    81841 atcttaattt tctcgttatt tattttctta tcagccgagt tttgtgagaa ctttatcgcg
    81901 ttatcgatga gtatggtaag tatctgttct atacgatcgg cattgccgta tacaagcaca
    81961 ggttgcgaag gaccggtaaa atcaattgtc aaattattat cctcataggt aaaatgataa
    82021 ctctcaatct tatcgacaat tattgccgaa aggttaactt tatccatggc gaatgcctcg
    82081 cttgccgact gcagacggga cagcataagc aaatcgttta tcagtctgtt cagtcgctgt
    82141 aattcgttgg acataattgc ataatacttt gtaatcttat cggggtcggt aactattccg
    82201 tctttaagcg gagccaataa tcccataaca cctgtaagag gagttttcaa ttcgtgtgat
    82261 acatttgcca cataatctct tcttgtctgt tccagtcgta tctcctttgt tatatcgctg
    82321 aatattccca ttgcacctat caacgcatca tttaccttta acggatttat aacgcacaga
    82381 atcatatttc cttgaaactc atatatgttc tcaatatact gctgttccga taaacattgc
    82441 tcaaaatcct cccataccgc ctgtatggga attatactgt gcttttcatt catattcatt
    82501 gtggatttaa agaatttcgg aagggcggga ttgattatca tgggagaaag cgactcgtcc
    82561 acaactaaaa gaccctggtt catggagttc aatactccct gcagaatatt ggtctcacgg
    82621 gttaaactgg ttattgtttt gcctaattgt gaggataggt gattcatggt taaagacaac
    82681 tggcctaatt ccgccggata ggattcgtcg gctttgacgg aaaaatctcc ttttccgaat
    82741 gcaaccgcta catcccttat ctccagaaga ggcttggtga tgcttttgga cattctgtag
    82801 atgggaaata acatcagtaa tgatacacct atcatggtca gcaccagagc attgtttatt
    82861 gttcttaatt cgcctgaata gtcatcaatg aatttgcaaa cagtgagaac cgcctggaca
    82921 tctccgtact gcgagaaaat aggtctcatg ataataaaca tatcggagtt gaactcatta
    82981 tcggtatttt tcaaagaaat atattgccct gcaagcactc cgcgcatatg ctttgacagg
    83041 ttatttctgt agaacttgtc catgtccgct tttgccaccg tctgagtctc tcttctgatg
    83101 gcgggagcat aagaatatac ctctacattc agtttgtccg aaacaaatat aaagcttctt
    83161 aagatagagg attcggcttg taatgctttg tcaaagaagt tttccaattg gtctcttgta
    83221 acccaatcac cggggataag cgaagccagc atatctgttt cctcttttaa tctttccagt
    83281 tgattggtta cgaatatctc ttccgatgca acggtgtaaa tgaccgtggt gataataagt
    83341 gaaatgacaa gtattgctaa taccagtaat actattctga taaaaaaagg tatgtcggaa
    83401 aacctattct tcattgcact caaacttgta tcctactccg tagatggttt gtatacactt
    83461 ttgtatttca gcaggaagtt ttgcacgtat acgtttaatg tgagtgtcga tggtcctggt
    83521 gtcacccgta tactcgtaac cccagattaa gttcataatc tgttctctgg taaagactat
    83581 cttgggattg gataacagaa gaaaaagaac ttccacttct ttaggtgtgg catgtgtggg
    83641 tacatcgtct ataaggatgg aataccttgt aatgtccacc gtaagttgtt tgaaagacac
    83701 cttcccggaa gactcttttt gagaaggatt tcttacacgg ttgattattg cttttattct
    83761 cgctataact tccagtgagc tgaaaggttt ggtaatatag tcatccgctc cgagatcaaa
    83821 accaaggagt ctgtctactt cctcggattt tgcagttagt aatataatgg ggatagtgct
    83881 gcttattctt attcttctgc atacttccat accgtcaata accggcatca ttatgtccag
    83941 cacaataaca tcaaaagtgc ctttttgata taaatccaga cattcctgtc cgttgaaagc
    84001 cgattgatgc gtcattccga acttgtcgaa ataaacgctt aaagcctcgt gtaccaaagg
    84061 atcatcatca cagattaaaa catgaatatt catacttatt cctctttaaa aaaattatac
    84121 caaacaaaac acctatattc aaacataacg ttaaatagcg aaaatcaaag gaaaatcaac
    84181 ggtcaagaat tatgtatatt attaaattgt agaaaaagtt atataaactg taataaaaca
    84241 ggtaataaag caataaataa catcattctt ttgagactaa caatgctaaa agtgttataa
    84301 taccctggag gatgaaaggc aggaaggata ttttatgatt tatgactttg ataaggtaat
    84361 cgacaggcat aataccaatt cgcttaaata tgatttttat gaagattccg gatacacggt
    84421 agatcatatt ccgctttggg tagcggacat ggattttccc gtcatagagg agataaagag
    84481 tgcgataagc agaataaatg cggtgggaat ttacggatat accggcatct ccgacagcta
    84541 ctacgatata gtcgccgaat ggatgtacag ataccataat ttcaaagtgg aaaaggactg
    84601 gttctgtata acgcccgggg tggtgtatgc attaagcgta gcggtatgcg catatacgca
    84661 gccgggtgaa agcgtgatag tacagacacc cgtgtattct cctttttacg gtgtcataaa
    84721 caacaataaa cgtaagttgg tcagaaatcc gttggtttat aaaaacggaa gctattatgt
    84781 ggattatgtg gattttgaga ataagattgt ggaaaatgat gtaaagctgt ttataatgtg
    84841 caatcctcat aatcctgtcg gaaaggtctt taccaaagag gaacttataa gaatgtgcca
    84901 catctgctgt aaacacggtg tgataatagt aagtgatgaa atacatagcg attttgtgta
    84961 tgcaccccat aagcataccg ttcttgccaa tctgaacagt aatttcgaac agtcttgtgt
    85021 aatatgcaca tcgcccggta agacatttaa tataacggga cttaaagcat ccaatatatt
    85081 tatacccaac ccggaactga agaaactgta tgatgaggaa atggcgaaaa gaggacccgg
    85141 tctggtcaat acgcttggga ttgccgcctg tgttgcggca tacagatacg gaagaccgtg
    85201 gctcaataaa ttactgaagt atctgcaagg caatatagag tatgttaccg attttattaa
    85261 cctgaatctt ccaaagatta aagttgttca accgcaaggt actttcctgg tctggataga
    85321 ctgtatggaa atgggtctgg aaaacagcaa actcaataac tttttcaaag atgaagcgaa
    85381 aatatggctt agtcccgggg ataaatacgg tccggaaggc gaaggtttcc aaagaatcaa
    85441 tatcgctacc agcagaagca ttctggaaaa ggcaatgaac agaataaaga ctgcttatga
    85501 tgtgttgttt tatgagagaa aagcaaacag cgacgataaa gagaccgata atcagtcggc
    85561 ggaaaatctt gacaacgagg ttcaatgatt ttattattaa ctaaaatatt acgaggatag
    85621 aaaaatgctg gacattaatc ggataagaaa taacagagaa gaagttgaga aagctctcct
    85681 gaaaaagaat ttcaagatag attttaccga acttttggat tttgatgcgc aacgcagaag
    85741 caaaatattc gaagtggagc agatgaaagc caagagaaat accgtttccg gtgagattcc
    85801 caagcttaaa aagcagggaa ccgatgttac cgatcttttg aatgagatga agaaaatttc
    85861 attatccata gcggaattgg aaaaagaaat aacatcaatt gaagcgcaga taaaagacat
    85921 aatggattat atacccaaca cacccgcaga cgatgtggtt gccggagatg cgacgcaaaa
    85981 tcaacctgtc agatatcacg gaaggaaacc ggagtttgat tttactataa aagaccatgt
    86041 tgctttatgt gaagcactgg gcataataga ttacaaacgc ggagccaaac tcagcggaag
    86101 cggccactgg atgtacagag gtgacggagc acttttagag tgggctttgc ttaactattt
    86161 cataaccgag catatcaaag acggatatga gttcattctg ccgcctcata tactcaatta
    86221 cgaatgcgga tatactgcgg gacagtttcc caaattcggt gatgatgtat ttaaacttac
    86281 cgaaaacgat cagtttttac tgccgacggc ggaaaccgca ctgataaatc tgcataggaa
    86341 tgaagtattg agagaagaag agcttcccaa aaaatacttt gcttatactc cctgttacag
    86401 aaaggaagcg ggcagttaca gagcggaaga aagaggcatg ataagaggtc atcagttcaa
    86461 taaagtggaa atgttccagt ataccgtgcc ggaaaaaagc gaagaagcac tcatggaact
    86521 ggtagccaaa gcggaggctc tgatgaacgg attgggctta catttccgct taagtaaact
    86581 ggctgcagcc gactgttctt tcgcaatggg caaaacatat gatgtggaag tatggattcc
    86641 cagcatgggt atatacaagg aagtaagttc cgcttccaat gcatatgact ttcaggccat
    86701 aaggggaggc atgagattta aaaggacagc aaccgggaaa aatgagtacc tgcacacatt
    86761 gaatgcttca ggacttgcca cttccagagt ttttccggca attatagaac agaaccagca
    86821 taaagacgga tcggtgactg ttcccgaagt attaagaaaa tgggtaggta aagataaatt
    86881 aacggtatga tagaatattt cagcgaaaaa acggactttt ataaacagca caagccttcg
    86941 gagctgctct cggcatacgg gtcgcctttg tatgtttaca atgaagatat actgagagag
    87001 cgggcaaggc agataaagaa ttttgtgaat atgcccaact ttaccgtttc ctattcggta
    87061 aaggcaaact gtaatctgca tattctgaag atattaaaag aagaaggact gcatgccgat
    87121 gccatgagcg aaggagaaat ctttgtaacg gaacaggccg gttacgaaaa agaccgtata
    87181 ttctttgtct gcaacaatgt atcggccgaa gaaatgaaat atgccgtaga caggggcatt
    87241 atggtaagtg tggattcact gtctcagctg gaactgtacg gaaagaactt tcctaaaact
    87301 aaagtagcgg tcaggataaa tccaggaata ggagcgggac atcataagaa agtggttaca
    87361 gcgggagagg ctaccaaatt cggtattaat atcgataaag caaatgatgt catacgcatt
    87421 gctgaaaaat ataatctgaa aattgcgggt ttgaatcagc atatcggttc tttcattatg
    87481 acttccacgg aatatatcaa aagtgtaaag gagttactgc aattatcgtc gctttttgaa
    87541 gatatagagt ttctggattt cggaggcgga ttcggtattc cgtataataa gcagcagggg
    87601 caacagccgt tggatcttaa acagttcggg aaagaactta aagatgtatt aacaagttgg
    87661 cagaaggaaa agaagaagaa tatactgttt aaatgcgagc cgggacgatt tgtagttgcg
    87721 gaatcgagta tacttttggg caaggtatat gcaaaaaagc ataactacaa caaaatatat
    87781 atcggaacgg atatcggatt ttccgtactc atccgtcatg caatgtacgg agctcaccat
    87841 gatatagaaa tatacagaaa tgacaaaccc gttaaaaacg gtgcttatga atttgtaaat
    87901 attgcaggta atatatgcga aagcggagat atacttgccg aagaaaggca attgcccgta
    87961 atattggagg acgatatcat aggtgttatg gatgcgggcg cttacggata ttccatgagt
    88021 tccaattaca acaacaggtt gcgtcctgcg gaagtactga taaagaacga cggcggttta
    88081 caggttatac ggaaaagaga gactctggag gaccttataa aaggtttttg atgttttgtg
    88141 gaaaatatag ctaagatgga atataattgg atatattcca ttttttttga ggtgaattat
    88201 gaacacaatg ataaaaaatg cagatattct gacaatggat gaaaaattta ccatattaaa
    88261 aaatcaggtt atctgcatag aagaggataa gatagtgtat atcggtgagg atgagccgtt
    88321 cttttttata cccaacagga tgattaatgc gaagaataaa atagttatgc cgggtctttt
    88381 caatgcacat acccattgcg ccatgacact gatgcgaaac cgctccaacg atcttcctct
    88441 ggaaaggtgg ttacacgaac agatatttcc ggtggaagcc aatcttacat acaaggatgt
    88501 ctacaacgga gcaatgctgg gaatagcgga aatgataaga gggggaacgg ttgcctatct
    88561 ggacatgtac tatatccatg aagcatcatt ggaagctata aaagaaacag gtataaaggc
    88621 caatctttct tacggggtat ccacatccgg caaggtaaag gaactgggta aacagggagc
    88681 ggagcagtac tgtaagaact ttattttaac caacaaaaat gcatttaaag gaaggcttaa
    88741 tacatcggtg gaaatacatt cggtgtatct ggtagatgag cctgaattaa tcttaagtgc
    88801 aaaaatggct aaggaaaccg atacccttat acatattcat ctgcatgaaa caaaggcgga
    88861 agtggaaaac tgtatcaaaa catacggtat gactccttta cagttatgtg ccaagaccgg
    88921 tgttctggat aacagggtta ccgccgcaca caccgttcat gtaaatgatg aagatatgct
    88981 gttgatgaaa gaaaagaatg tcataccggt acacaatccg tcaagtaatc ttttgctcgg
    89041 cagcggtatt gccgacatac ccaaaatgct taaagcaggt attaaacctg caataggcac
    89101 agacggtgcg gcaagtaata acactttgga tatgttcaag gaaatgcatc ttacttcact
    89161 tatacacaga ggttataaag gtgacagtaa tgttataaat gcacggcagg tgctgaccat
    89221 ggcaacggcc aacggagcaa gagcattggg atttgataag tgcggtatga ttaaaaccgg
    89281 tatgaaagcc gacattatac tggtcgatac cgaaagcctt aacatgacac cgttccatga
    89341 tccggtatcg gcattggtct attcagtaag agcaaccgat gtggataccg tgatggtaaa
    89401 cggtgagatt cttatgaagg ataagcagtt gctgactatt gatgaagaga aggtaaaata
    89461 tgaagcagcc gggtcggcag caagactata taacttattc tgacaaaaaa ataccggata
    89521 atgctgaaaa tgctttataa aaatgataga ataaagaaaa acaggagggt gaatatgtac
    89581 agtacggaaa aagcatataa acaggctaag gagtattata aacgtttcgg aatagacacg
    89641 gaaaaagcaa tgaaattatg tgacaaggtt ccgatttcaa tacattgctg gcaaggcgat
    89701 gatgtgggcg gatttgaaaa gagtgcatcg gacttaaccg gaggacttca ggtaacgggc
    89761 aattatccgg gcaaaccgag gaccgccgat gaattacggt cagacctgga tgttgtaatg
    89821 aaatatcttc cgggagtcaa aaaaataaat ctgcatgcaa tttatcagga agcggaggaa
    89881 tttgtagaca gagatgccat aaaaccggaa cacttcaaga aatgggcgga ctgggctgtg
    89941 gacagaaata tcggactgga ttttaatccc acatattttt ctcatccttt gagcgaaaac
    90001 ggcacgctgt cggctgccaa tgatgaggta agggagttct ggataagaca cggaatagcc
    90061 tgcagaagaa tagggcagta tttctatgaa agaacaggga aaccctgcgt tgtaaaccac
    90121 tggacgagcg acggttccaa agaaatacct catgatactc tgtcaccgag attgagatta
    90181 aaagacagtt atgacagaat atttgcggaa acagccgatg taaaaggcgt catagacggt
    90241 atagagagta aattgttcgg aataggattg gaaagttata cagcgggaag ttttgagttt
    90301 tgtctgagtt atgttattaa caagagaaag aatcacatca ttcttacgat ggataccggt
    90361 cattatcatc ccaccgaggt ggtatcggca aaactgggag cgatattcgc tttccaaaag
    90421 tcgatactct tacatgtaag cagacctgta agatgggatt ccgaccatgt agtttctttt
    90481 gatgatgaga ccagagctat atttgaagaa ttggtaaaat tgggcaaact taataacact
    90541 tatgtagcaa ccgactattt tgatgcttcc atcaacaggg taatggcatg ggttgtgggt
    90601 ttaagaaata ccagaaaagc gatacttgcg gctctgttac aacctaacga aatgctttcc
    90661 aaggtagcaa caagaaatga taacaccggt gtattggcaa tcagagagga gttcaaatcc
    90721 gctccgttcg gtcttgtttg ggattactac tgtgaaaaag ccggtgccaa atcgggcatt
    90781 gaatggatta atgatataaa acagtacgag caggatgttc ttttaaagag atagtattta
    90841 actgctaaag aagtattaag aagaaaagag tacatagaac aaaaaaaccg gtatacccgg
    90901 ttttttgttt cgatattttt atacaaatca atgtcttaca taatatttag aaataccgta
    90961 aaagtagcgg aaatgactat ttcttcacat gtctcaatcg gaacatttcc tgttcatcca
    91021 gcacgctgtc ggaaaaaggt gacagggtaa aggaaaagct gttcggattt gtataaagca
    91081 ggtacttgtc ttccgggccg gggccgcagg aagcggtacc caatcccgac tgtttgtaat
    91141 cgatgtttac ggtgattcct tcactttctt ccaactcatg taagtgtcgt gcctttgcct
    91201 tctgatatgc atcatgatag gaagcggaga agttgaatgt atccagtccg ccgaagaaca
    91261 ctccctgtcc ctgaacattc ttgaagcttg cccatctggt gtccattctg ttgccgtttt
    91321 cctgaggttt ttcataatgc tcctgtaaat caccaataag tccctcatat attcccacaa
    91381 cggcactgct gttcatgtct tcataactct gatggaaacc tcttccgaac catttgacat
    91441 attcatattc aaaaggtatc tgcatggtta atccgaattt gggcagatac aggcattccc
    91501 gattaccgtc tgtattttga aattttataa aatcattcaa cggctcatat cccgaattaa
    91561 cggtaatctg accgttggta tatacggtat attccgtaac tgtttttaat acaggtttct
    91621 ttgtagttgc ggcaaatacc gatacaactt taaaaaccgc tttctttgta tttgctttga
    91681 atgaataact ctctatacgt ttctgcagtc tgtctaaacc gtactcctcc cattttttcg
    91741 ctattttgac atcattgtct ccgggcgctc tgtagaatga ctcgcttgtt ccggtaccta
    91801 aaagcatttc gtttccgttg aagtagtaag aatagagatt accgaacatt ttggagaagg
    91861 ataccttgaa actttctcct aaaactatat attccgttct tgtttccacc caggcaagat
    91921 catttctttc caaaggcttt ttatactcga acttttcatc aattattatc tgtgacctgg
    91981 ttacttcata tcctttttcc gcaaacggag tgctttcgtt gtattcaaag aagaatgtca
    92041 gctgtacatc accgaatatg tttgcatcaa actccagtgt atatgtcttg gtttcctgag
    92101 gggcaatgtt aagaccgggg atatttccga cttttagtat attgccgtct ttagacagtt
    92161 cataatacac ccctatattg gatatatcgg caaaatccag catgttttta aaggtaaccg
    92221 tattggtgtg tttatcgaaa tttataactt taaccggttc gtaaaccttt cttaactcta
    92281 tcagagcggc tttgggtatt ctgtctgcgg tattatagcc gtccagacag aaattaccgt
    92341 cattgggaaa atctttgaag tcgccgccgt atgaaataaa ggggaccatg tcttcggtat
    92401 aggcaagaag accgtgatcc gcccattccc agacgcatcc gcccatgagg ttgggatatt
    92461 tgtatattat atcccagtaa tctttcaatc cgccgggacc gcatcccatt ccgtgggcat
    92521 attcgcaaag gaagaaaggt cttgtatccg aagcggcacc tatttcttcc agtttttcat
    92581 gacttgtata cattgccgaa accacatcca ccgattggtc tgttaaggca cgttcatagt
    92641 gaacgggacg attgtcaacg cttctgatgt aattaatcat ggctttatgg ttattgccga
    92701 accggcattc attacccaat gaccacatga taatacaggg atgatttttg tctctttgca
    92761 ccattcttgc cgctctgctg acaaaatgat gtgtccactc gggtttatcg gataaatcat
    92821 attccgggtc attgtaataa aatccgtgag tctctatatc tgcctcatct ataacatata
    92881 tgccgtataa atcgcagagc gagtaaagat acgggtcgtt gggatagtga gaagtacgta
    92941 tcgtatttat attatgcctc ttcatcatca ctatgtcgct ttccattact tcacgtgata
    93001 tggcatgtcc ggtcaatgca taagtgtcat gtctgttggt gcctttcagt ttaaccggtg
    93061 tgttgttaat catgaataca ccgtcattta tagaaactgt tctgaatccc actttacacg
    93121 accgtatatc ggtaaccgtt ccttttgcat cctttagttg caggataagg gtatataaat
    93181 tgggtgtctc gcttgtccag ggcagaactc cctcaacatg catatctata aagggtttct
    93241 ttacggattt gccgaatatt ttgacatcgt ctttatcata cagttcataa acatatgtaa
    93301 taccttttgt ttcggatgaa agaatactga caaaaatgtc gttcaatgtt gttttgaccg
    93361 taaaatccat aatggaattg ttgtgtgtgg aatagagata tacttcacgg aaaataccgc
    93421 tcagtctgta aaagtcctgg tcttccagat aactggtggc actcatctta tacacctgta
    93481 cggctataag gttggcaccc tgacgaacat atttagtgat atcgaattcc gaaggcatat
    93541 gcgagccttg ggaataacct acttcctgac cattgatgta aagaataaaa gaggagttaa
    93601 caccgccgaa atgaattatg acatttcttg cggcaaaaga atcgggcagt acaaatattg
    93661 ttctgtaaag tcccacaggg ttgatatcgg gaatatgcgg aggatcgacg gggtacgggt
    93721 atcttacatt aatgtactgc ggtttatcgt atcccaatac ctgccaggat gaagggacgg
    93781 gtataatgtc ccagtcttca tcatcgtatt ctggaagata gaaagattca tccacatcat
    93841 attcattctc gcagtatttg aagttccatt ttccgttcag aagtttaaag taaggtgata
    93901 accccctgat atctttcttg gcatcggcga catcgctgta aggtatcgaa gttgctcttg
    93961 gagcaagtac atttttccct aaaattgata tgtctttgtg gtattttttc atggagacct
    94021 cctcatattt tattgacaga cggttattga atcaatacat tcaacaacaa cattatatat
    94081 cttacatcac gtatgtgaaa gttatgtgaa aaggaaaaag gtttttaagt aaacaagttt
    94141 aaagttaaat aacttaccgt taatatcaga taattaatat gtggtataat gcttgaaggg
    94201 taacactatg agcaattcga agaaaaaaaa gactgataag gaaaagaaag cattaactgc
    94261 aatcaccgtc ataaccgtag ttgtgggtat accgcttttt gtacttttcc tttcccgata
    94321 ttttacaaaa aatacgggta tcagtaccga tactcccgta attcagtcac cgattgctac
    94381 atatcagcaa acggaaatac ctgctgaaac agttgctccc acacagaccg ttatgcctac
    94441 ggaaaccgcg gctgttcaga cacctgaacc tgtaccgtca tcaagtataa acttaaacga
    94501 aattgaaata cgaaacat
//
LOCUS       DAOSOM010000005        82617 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_294_length_82617_cov_5.361498, whole genome shotgun sequence.
ACCESSION   DAOSOM010000005 DAOSOM010000000
VERSION     DAOSOM010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 82617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 82617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82617
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_294_length_82617_cov_5.361498"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="PLF14_02280"
     CDS             <1..529
                     /locus_tag="PLF14_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003871201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HOT70157.1"
                     /translation="NDIDVKYIKQGLSDVVVKGRCEKVDTGKDFTVMIDYAHTPDSLE
                     KLLIAVRETRTSGRIICLFGCGGDRDKSMRPMMGKIAGKNADFSIITSDNSRTEKPDD
                     IISMIEEGIKETDGEYICITDRTEAISYALRNAKKDDLIILAGKGHETYMDIMGKKTH
                     YDEREIIQKVLKGDI"
     gene            526..1881
                     /gene="murF"
                     /locus_tag="PLF14_02285"
     CDS             526..1881
                     /gene="murF"
                     /locus_tag="PLF14_02285"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOT70158.1"
                     /translation="MRIELGRIYDLFATGLKTDKKTVVTGVSIDSRTIQPGEIFFAIK
                     GENFDGHNFVNKALEKKAAAAVCSRKDKYAGDNIIYVEDTVTALQDLARFYRRSLDIP
                     FIAVTGSNGKTTTKDYTGAALAASFKTDYTKGNLNNQTGVPLTVFSVGEDSKAAVIEM
                     GMNNAGEIRQLSSVVLPDIAVITNIGTSHIGNLGSRENIFKAKTEVLEYMDNGYLIVN
                     ADDDMLRRVKNNHKYKVIRVGILADDLDYRARNIVKNADNTYTFSVTDTKIKLSIPGI
                     HNIVNALLAFAVADILKADRKKAAQAIGACTNQVMRTQISEIDGYTVINDAYNANYDS
                     MRMAIDILKNYRGRKVAVLGDMLELGDFEEMMHERTGEYLAYNKIDVLIAVGKNADCY
                     KKGATDFGMEEKSIHVFPDIPQAKKHIMDIICKGDTILVKGSRGSSMEKILEVFEGDF
                     V"
     gene            1878..2831
                     /gene="mraY"
                     /locus_tag="PLF14_02290"
     CDS             1878..2831
                     /gene="mraY"
                     /locus_tag="PLF14_02290"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOT70159.1"
                     /translation="MIIAISASFILTVILGIVLLPVLKRLKIGQVVRDDGPKDHLKKA
                     GTPTMGGIIFIFPIIGCMLFLGWNNPVVLVTCGLILLSALVGFLDDFLKIKRKNKDGL
                     KPLYKTLLFILISAAYGIYIVYFSKIGSEIIIPFTNAGKTFVLPGWIYVIFIVLFYNA
                     VINAVNFTDGVDGLCGSVTAVVFVFFAIASKIVFDDTDMTYMSLIIVGAMVGYLIFNI
                     YPAKLLMGDTGSLALGAAVTAISVNLRIPWIVLFIGCIYVIEILSSVIQVMHYKRTKK
                     RIFLMAPIHHHFELKHWSENKIVIVFSAVTLAGGIIAFLAI"
     gene            2842..3960
                     /gene="ftsW"
                     /locus_tag="PLF14_02295"
     CDS             2842..3960
                     /gene="ftsW"
                     /locus_tag="PLF14_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HOT70160.1"
                     /translation="MKNGEKKKADFLILLFPLALTALGLLMIFSASAPYAKSQFGDIY
                     FFIKDQMIAALIGIVAMMGVSFINYKLYKKFSWVIIAGGLVLLLLTFVPGIGVEHNDA
                     RRWISIAGFEFQPSELYKLAFIIFLSKIIVDFKEKEKPLAGYFTTLAITLASAVIIFL
                     QPHLSAAIIVILIGFIMIYAGGTKLKYLILTVAPVVALAIIGAFTVNYMKDRIMTWLD
                     PFAYPTDSGYQVIQSLYAIASGGLFGVGLGKSTQKFLYIPEPQNDFIFSILAEELGYA
                     GVFLVIALFCMLLWRGIRIAIFCEDLFGTLLATGITALIFVQAALNIAVVTGLVPNTG
                     IPLPFFSAGGTSLVTFLIEIGILINISRQPIKKERLTA"
     gene            3957..5054
                     /gene="murG"
                     /locus_tag="PLF14_02300"
     CDS             3957..5054
                     /gene="murG"
                     /locus_tag="PLF14_02300"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOT70161.1"
                     /translation="MNILIAGGGSAGHINPALSIAKKIKNNHPGYKILFIGSEKGLEK
                     DLVPREGFDIEFIEIEGFKRPFGLRSIKTLFVLIKGLFQAGKIIKRFKPDIAIGTGGF
                     VSGPAIYISSLYKAKILIHEQNAYPGITNRMLARRADVAMVSFESALKSLSKAKKTVL
                     TGNLIDESFSDFDKYQPADGMKTVVIIGGSQGAMTINNAVTGMINEYMKETDFKLIFA
                     PGKRHYEEVTGKITKTLKNVTVHDYIYDRNTVYASADLMVTRGGATTISEVLAMGMPS
                     IIIPSPFVPDNAQEKNAEQIYEANACEIIYDNSEFNSKVLYDKIMNLLGDKEKLIALS
                     ENAYSLGIRDANQRVYEEFSEVVNEINRSGK"
     gene            5029..5763
                     /locus_tag="PLF14_02305"
     CDS             5029..5763
                     /locus_tag="PLF14_02305"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT70162.1"
                     /translation="MKLTVLGNNGPFPAKDGACSGYVVENNKDCRSGNKTLDSKGLVI
                     LDLGSGCLANFQKKFDIEDISFVVITHLHFDHMSDLLVLRYALQVKGRKMDIYLPKEP
                     ENVASILKCDEFNLIYIDESSVIERDSFILNFKQTTHPVETYAVKINNHFVYSSDLAQ
                     PEDLKDFACGVDTLLIDSALLAKQKSKTNAAHLCAKECGMLAAELNVKRLILTHFPPY
                     SDLRQYLMEASTYFANTVTAEILQEY"
     gene            5896..6897
                     /locus_tag="PLF14_02310"
     CDS             5896..6897
                     /locus_tag="PLF14_02310"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOT70163.1"
                     /translation="MIKKAYISILLIILLICLTGCMSIFPKEDEMLLLPVPSTAPVEY
                     KTINPTKGDIVNSITILCNVANRYENLQDQFFSVDGYLEELFVENEQYVEKGQLIAVL
                     SDREDREQVENTYLNALNEFIKVQDLYVKNAATEYQFRRAEIAYNHAKEKYDDLYTYM
                     TNKELRASQSGFIVNIRSFTNDIAKGTPIGKTIRFCSIATGQYKSISGTASAANASKL
                     KPGASVIIVSKEIEYDGYVLTSEGTNLSFGLDDMDAVTAGEVVQIKVIIDEVYDVLKL
                     PKYAVTIRDQKTAAVRVLKDNTPTNMEINIGLASSSEVEILPGQGIDEDTMIITGTN"
     gene            6899..9733
                     /locus_tag="PLF14_02315"
     CDS             6899..9733
                     /locus_tag="PLF14_02315"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOT70164.1"
                     /translation="MLRLIFRKILNNKWMVICLIIGCAFAIGVAAAIPMYASGMYNTM
                     LVNEFKKEQVQFDTFPFKASYNVTFFGLGSDTSKRVYENLDNTFYNKYMANMPVEVMK
                     DCNSVATKTYSVLDQNNYRIKQDNLLKEEKPSYRNFTIKTDFCLTSVVDAETVFDVVG
                     GRIMQTGTEEGVIEAVISEYAAALYRMSVGDYYFVYNKPDDDDFIAKIKIVGIVKVNE
                     SSASFFSVAERAFIIDFDIMQEMLKESRITLNQANWFAAFDYTTVRLRYANDISQATQ
                     ALSTSSSVTGVIINTSNNYISIISNYNRDMQTMQKMFLLLIIPVILMIGMYVVMISRL
                     MMDREKSEIATLASRGASRNQIFLIYLGQGIVIAVVSFVLGILLARLLCHLLGAANGF
                     MEFVNRTAIEVRFESTSLLYAVVCALIYLIMILVPAYKASKYTIVQLKQSIADQSRRS
                     FWQIMFLDVILIGVSLYFLYDYEQMSKYIQDVQSAQVDFTLFLAVTMFILGLGLLFIR
                     LFPYLTELVFIIGKRHWKPASYNALMHVSRGGNYKHFIMIFLILTIGVGIFNANSGRT
                     LNENLEDNIRYEIGADASFVPTLIVLNDKGTFDYKPIKGAALMSFARLYCNSDFVENY
                     TTYYYYKTETRPSREPIVFMGIEPYEFGNVVKLRKDLNEGIPINHILNTLSSYPQGMI
                     ISRSLANEYGVKEGQFMNYVYTSVIEGVTVENLVENALVVAVVDYWPAMTDERFIVMT
                     RDNYFVMKDFDTRGRILIDKKDGVLDSQVLADIKGKMGPEETIDSINFTDYNVTLAKK
                     NSILNGMNGILTLDFLISMIVCCIGFIIFWIISVRQRVLQFGIFRAMGMNKGELYRMI
                     FYEQLMISFVSVIAGIVIGGVASQLFVPLFERMSTLSNQYLPFATYRDGIDYIRIYVI
                     TGVSLIIGLSVLIRFISKLKIDQALKLGED"
     gene            9733..10407
                     /locus_tag="PLF14_02320"
     CDS             9733..10407
                     /locus_tag="PLF14_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016741088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT70165.1"
                     /translation="MAVLLRTVDICRDFKQGSQTVHALVDVNVSIQPGTLTMLAGRSG
                     SGKTTLMNIAGALDRPTSGQIFFHDREISDLSENKRDEIRRKSMGFVFQSVALISVLN
                     AYENVEFGLRMAGVHPKKRDRLVKEALTFVGLERRMKHKPHQLSGGEQQRVAIARSIV
                     ARPELIFADEPTAELDTKTGLRIVRLFRHLVDEEKLTIIMTTHDPSMIELADNVIRLE
                     DGRVVK"
     gene            10533..11426
                     /locus_tag="PLF14_02325"
     CDS             10533..11426
                     /locus_tag="PLF14_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT70166.1"
                     /translation="MIFCDGLVKIYKSKDTEVVALQGLELEIKKGEMMAIIGNSGSGK
                     STLMNLIGGLDVPSAGTLIVDGENLLRMKKSELLRYKKEKVGFVWQNNARNLIPYLPA
                     IHNVEIPMILAGRKDAKKRAAELLDLMGLKDRMKNRLSQLSGGEQQRVAIAIAMANDP
                     KILLADEPTGAVDTATAALIYNIFRKLNTEYGVTIIIVTHDRAISKNVDRVVTIEDGR
                     TGSEYIKKKSYKEQIMELDRMTGVEESETHEEMIVVDRQGRLKIPEEYLELAKIPNNS
                     KVRIKMEEEKIVIYTEEKDEE"
     gene            11416..12540
                     /locus_tag="PLF14_02330"
     CDS             11416..12540
                     /locus_tag="PLF14_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOT70167.1"
                     /translation="MKNKSFIILFIIAAVMLSFLTGCTYFYPLEGRYHAVQPELIEQK
                     KVELDSFEVRKTDVFEGFDLGEEFGRRLVYVNNPKKTTIVQSEIARAKIQKLYVEPND
                     YVMEGDILVEYVYEVNKDAEFQVNWNKRRAEIRYENAYIQYQQGKINEQTLQNLQKAY
                     AVAKNALDSFNAIPDKYTLRAPVDGYIAKIEGDTVSTDEVQTLVFHICKIEDGILLTY
                     AREQNEYAAYPFTLCFEGTGMELYDEQSDRTYSGRVSMSNMTFKKEYVNDLSYFSGGA
                     YLYLALELNDNKLPEDVVFYQEFTGKLIRREAYDVLIVSRSAVDMEKTDDPEEPFVYQ
                     VYKILPDETIERVRIKIGDIFDAYYEVLEGLEEGDKVVLR"
     gene            12537..13625
                     /locus_tag="PLF14_02335"
     CDS             12537..13625
                     /locus_tag="PLF14_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70168.1"
                     /translation="MIEKRTNLLISIIIIIVFTAILTSCATQAVTYEIPVFDIPKEVF
                     TTLKVEKQDIHDYERINLDAQSLGGSRGIVFSYSDFESATLLVSEQNTVVKEGDVLAV
                     KSDKTLEYDLRLKLEDLELKQLLYEELYRKYLNTGEGYYDMQLALSDMELAQFNYDKA
                     LEKLETLQFKAPYSGTVNKVVTTYKNEKDEFNNQYKVYSVTYYITPDNYSVSLQITAA
                     GYPYFEELGYKSGDTLQIYDKDGKIHNMIIKSIFTRTTELAGSKIKTAFVYLEFIKEE
                     GVDYGELIKYALFYDMTLAKYDDVIVVPVSYVRKTSEGKDFTYVLENGRKVPRTLIVG
                     NKVLNGTAYLVLDGLYEDDLIITGSSFE"
     gene            13694..16531
                     /locus_tag="PLF14_02340"
     CDS             13694..16531
                     /locus_tag="PLF14_02340"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOT70169.1"
                     /translation="MLRLIFRKIWNNKWLVICLIIGCAFAVAVVAAIPMYTTGLYNLQ
                     LSEEFKKLQEEFDSFPFKAYLNASFLGMKKSDSIKTFNDLDSKFYDRYVANIPAKNIF
                     DYKSISTVSYPVFDKRNYEIKQDMIARGEKLYTRNLTIKSSFTITSFAKVPDMIKLVG
                     GRMYSTDTEEGVVEAVITKNSTIMNRISVGDTLYVYNKMTDIDLLVKIVIVGVVENTD
                     ESAAFYTLSDKTFLIDYDKMLEMIGNEEITLQSANWFCAFDYTTVKLRHSDRIIAATK
                     VLDSYDADSKVMLSSSNSYMTLLEKYSEDVKAMQRLYILLLLPVILMVGIYVVMISRL
                     MMEREKAEISVLASRGASRSQIFLIYLAQGIVISVISYVLGILIARFLCRLLGAANGF
                     MEFVNRTAIDATLTSAALLYAAICATTYLILILVPAYRASKFTIVQHKQNIAEQSKKS
                     FWQIIFLDVILIGIAIYFLFDYESVKEYIQFAQITSVDFTLFVAVTFFVLGTGLLFIR
                     LFPYLVELIFRIGKERWKPASYAAFTHVARGGNYKQFIMIFLILAIGIGIFNANAGRT
                     LNENLKDNLKHSIGADISFVPSVLEYDFILNGYYFADNRGPGLSKQAHKYCNSDLIEN
                     YTVYYYHNTTLRPSNQSVTFMGIEPGDFGRVVKIRKDLNEGVPLNHILNILSTYPKGI
                     IISRSISQLYNVKAGQYFDFTYTNRAFREVQEIPVKDVLVVAVVDYWPTMTVDNFIIM
                     TQNKYSSMRHFRQNGKVLVDKKEGVSDNEVLEDMKTRMSFDESIGTNSFTDYEATQAK
                     RGSVLNGINGILTLDFLISMLICCIGFIIFWIISVRQRVLQFGILRAMGMKKHELYRM
                     IFYEQLMISFVSVLAGILIGGIASKLFVPLFERMSTFSSNFLPFYAYREVVDYNRVYI
                     ITGAALTIGLSVILRFTSKLKADQALKLGED"
     gene            complement(16584..17858)
                     /locus_tag="PLF14_02345"
     CDS             complement(16584..17858)
                     /locus_tag="PLF14_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70170.1"
                     /translation="MNKKLYLTVAVLLILCFVLSSCRNETQISFTPTNTVESDLVILQ
                     PDLSLPDEYYTSMNQGARVWHSYLNTPYGKYHYVDGFILYSQYGNSKFVKLCTKAECT
                     HTDDDCDAYLGKIDQIVPTNEKTIQLGYYENNIYYVERSKKNGLDWNLIKMNMDGTER
                     QLVKTFATVSLTQGKRCLGFFHSGYYYFVMINAGQEMGSKYNTDNNFYRVKLDDYSEY
                     ESVISHDVIPEACMFTLVGDNVFFYVRTGPYNENYKLYDENPAEYELYMLSLTDKNMR
                     LLTDKWADYRNSHFDWENAYCYKHNDGFYELNLTSEQMNKVADFKIEDAWGSLAYFYP
                     DYNYIIAFAKTSDSRGYPRYFDSQTLYILDKQYHILNSCIIDALKLDTGCFIDDTGIS
                     VLLASSAHKPIDFFIYKSDIGTDNVKLNEITD"
     gene            complement(18012..19247)
                     /locus_tag="PLF14_02350"
     CDS             complement(18012..19247)
                     /locus_tag="PLF14_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70171.1"
                     /translation="MNKKLYLAVAVLLILSFILCSCRKEAQIVPAPTETEDTGLVILQ
                     PDLSLPDEYYTSMNQGAKIWHCYLNTPYGKYHNVEGFIHYSQYGNTKFMTLCQKENCP
                     HKYDDCDAYVGKNTQIGYYHGNIYFVQRAGNMLMTLYKMNMDGTERQTVKAIITDIQL
                     SIKEMHGFFHSGYYYFVLTDDQYMGSRFNTDKNLYRIRLDDDSPYESVFCHDIIPMAY
                     RFTLVDNNVFFYVRTGTYTEGTKNPENFAVDFELYKLSLTDNELCLLTDNWSDFTNCY
                     FDSENGYCYKHNDGFYVLNLATKEKTKVADSAIKGGWGTVAHFYPDNIYVIAFKKTKN
                     NRGHDRYYDDQTLYILDWEYNIVNIYNNPTSSTEAGFLIDDTGNCIVLSTDSHKPPDY
                     FIYKSDFGMKSLKLYEITH"
     gene            19456..20358
                     /locus_tag="PLF14_02355"
     CDS             19456..20358
                     /locus_tag="PLF14_02355"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HOT70172.1"
                     /translation="MDKKGNKLGEWINNELPEIASQLEALNDDYYLRENTISLAGKER
                     VKNILFLLRSALFPGVYEKCSISKANVDMLIGNNLREAAIELREILESVFSYKCDKDC
                     DNCPINLDEIIITFLDKMPKIRRKLQTDIHAAYVGDPAAQSFEEILLSYPSLEMMSIH
                     RIAHELYKLKVPLIPRMMNEYGHRVTGIDIHPGASIGEHFFMDHGTGIVIGETTEIGD
                     NVKIYQGVTLGAKSFKLDANGNPVKGIKRHPKIEDNVVIYSHATILGGDTVIGHDSVI
                     GASVWLMESVPPYSKIYYNLGSDK"
     gene            20362..21351
                     /gene="galT"
                     /locus_tag="PLF14_02360"
     CDS             20362..21351
                     /gene="galT"
                     /locus_tag="PLF14_02360"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HOT70173.1"
                     /translation="MAELRWHPLIKDWVMVASHRQNRPQMPKNWCPFCKGSEKVPKDY
                     IVMKYDNDFPALSQNPPIPDNVATDLYKTAPLYGKCEVILYSSDHHATLPELDDLHLN
                     ELVKLWKQRFIEISSDKLIKYVFIFENRGEAVGVTMPHPHGQIYGYPFIPKKLELELQ
                     SCKEHADNTGRCLICDMNGEEISFAQRVIFQNDSFIVYLPFFTEYPYGIYISSKRHIQ
                     YITQFTEKESSDFAKTLKIAAGTLDSLFGFTFPYMMCMHQAPVNTGEHYDYYHFHVEF
                     FPPMRARDKIKYNASSETGAWAHCNPTSVEEKAEELRAAYRRFVTGKGNTKGE"
     gene            21355..22518
                     /locus_tag="PLF14_02365"
     CDS             21355..22518
                     /locus_tag="PLF14_02365"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013411714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOT70174.1"
                     /translation="MIKEELANILNNVYGSSEESIRYFQAPGRVNLIGEHIDYNGGYV
                     FPAAIDKVSTVAVRKRKDDIIKLYATDLGYVVEADMNSLEQYKDLKWGNYQLGVIDQI
                     LKKGIKVKGAEFIFDDKVPLGSGLSSSAAIEVVTAYAMLQLYGHKTDKMDIALLCQKA
                     EHEYVGVKCGIMDQFASAMGLKDHAMLLDCNSLEYEYIPIKLDDYSIVISNTNKKRSL
                     GSSMYNKRRAECEQALADMQKKFPDKLNLCDFTYKEFLSCADDITDADCRKRAEHAVC
                     ENLRVLDSVKALKKGDIYSFCKYLNLSHISLRDLYEVSCKELDVLVESAWMQKDVLGS
                     RMTGAGFGGCTVSIVKKQSVKDFIEDIGRVYQEVIGYNASFYICEISDGVKEI"
     gene            22519..23520
                     /gene="galE"
                     /locus_tag="PLF14_02370"
     CDS             22519..23520
                     /gene="galE"
                     /locus_tag="PLF14_02370"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOT70175.1"
                     /translation="MSILVTGGAGYIGSHMVLHLLEKGYEVVVVDDLSTGNKEAVLAN
                     KFYQTDIKDEKAMAKLFKENEIEGIIHFAAYSLVGESMAHPLKYYENNVAGTLQILKY
                     MDMYGINNIVFSSSAAVYGESPSPIKETADTFPTNAYGETKLAVERMLKWASKAYGIN
                     YAALRYFNAAGSHVSGRIGEKRAVETHLIPIALDVALGKIDKLLVFGDDYDTFDGTCI
                     RDYIHVSDLATAHLLALEKMINDSINLERKSGNSGISMIYNLGSEKGFSVNQIIDAVK
                     HVTGADIKTEITKRRQGDPAVLIASSEKIKKELGWVPEFTDIEDIIATAWEFRKKMR"
     gene            23530..23826
                     /locus_tag="PLF14_02375"
     CDS             23530..23826
                     /locus_tag="PLF14_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HOT70176.1"
                     /translation="MNKKLKSKETDYLFDAILSLKNKDECYIFFEDLCTVAEIKAMAQ
                     RLCVAKMLDNKHTYADISVKTGASAATISRVAKCLNYGAEGYTTVLKRLGEING"
     gene            23819..26590
                     /gene="secA"
                     /locus_tag="PLF14_02380"
     CDS             23819..26590
                     /gene="secA"
                     /locus_tag="PLF14_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOT70177.1"
                     /translation="MGRILDKIFGTYSDREIKKILPLVEDIEALADEMKGKTDAQLRA
                     YTNLFKQRLEDGETLDDILVEAFAVCREASTRVLHMTPYKVQLIGGIVLHQGRIAEMK
                     TGEGKTLVATLPVYLNALSGKGVHVVTVNDYLAKRDSEWMGKLYNFLGLSVGLIVYGL
                     TKEQRENAYNSDITYCTNNELGFDYLRDNMVTRKEELVQRDLHFAIVDEVDSILIDEA
                     RTPLIISGAGEQSSNLYKRADNFAKTLKKKVVIEEDSGGKLQRIMELTGQEEEDEYAP
                     DKPDYIVDEKNRTASLTPKGVEKAERYFNVENLTSYENTGLLHHINNAIKAQSIMTRD
                     VDYVVKDNQIIIIDEFTGRMMFGRRYSEGLHQAIEAKENVKVERENKTLATITFQNFF
                     RMYGKLSGMTGTALTEEEEFREIYKLDVIAIPTNKPVIREDYPDVVYTTRKAKLDAIV
                     ADIIECSQRQQPVLIGTISIEKSEELSLMLRRRGIKHEVLNAKHHEREAEIVAQAGKP
                     GAVTIATNMAGRGTDIVLGGNAGYMAMQKLKKEIFDENILLQADSFNETDDEAVIEAR
                     NLYRQYEADFKKLIEEDRKRVLEKGGLYILATERHESRRIDNQLRGRAGRQGDPGMSR
                     FYLSLEDDLMRLFAQDRLANMMNVLNVPDDIPIEAKIITNTIETAQKRIEGVNFQRRK
                     TVIQYDDVMNTQRNLIYSQRRQVLDGTDMKGSYLKMLESVLTRLTDDYCQSNRSNDWN
                     MQAISDRLNEMLPKQYNPLLKGNTADADYNEKSFRQMLIDSYSKDYDDKEAELSPSLM
                     RELERIILLRTVDIHWMDHIDAMDQLRSGIGLRAFGQKDPVMEYKFEGYMMFEEMTNS
                     IQEEALKKIFSVHKQTEVKNEESKIRINAQTAPDDVRKPAVSSNTKVGRNDPCPCGSG
                     KKYKKCCGA"
     gene            26594..27874
                     /locus_tag="PLF14_02385"
     CDS             26594..27874
                     /locus_tag="PLF14_02385"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOT70178.1"
                     /translation="MNYEEAIKYIHSVGKFGSKLGLINITKLLDLLDNPQNDLKFIHV
                     GGTNGKGSTCNYIAHMLHAAGYRTGLFISPYIEKFGERMQVDFKNIDNRSLVAHVQKV
                     KDCIDMMLEQGYNHPTEFEINTAVAMLYFKQKRCNTVVLEVGLGGRLDATNVIKSPLV
                     TVICTIEKDHMGVLGDTLQKIAFEKAGIIKPASPVVVYGDNEPCTYDVIEAQAERKGA
                     PYFVVNFENISNIRYSDFGYTFDFEGYTDLRINMFGDYQVKNACTAIKAVEASKLKVS
                     EEAIRKGLFNAKWPGRLEVLSKNPLIICDGSHNPEAVRSLKNTLIKYFPDKKKILIMG
                     VMKNKEYEKMIEDIFPLADMGIAIRTQCNRALELDILQQTMQKYCKNTYSFDKIEDGL
                     DYAVANADNNTVICTFGSLYYIADVKNYVRRTGL"
     gene            27875..29191
                     /locus_tag="PLF14_02390"
     CDS             27875..29191
                     /locus_tag="PLF14_02390"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT70179.1"
                     /translation="MDINELQKYLEKIDFNALSKDNVKLSEDDAYVGELYNSAIDNIS
                     QYNIDVARIKLSRVVKLDPTFEKAKILLDKINSLGSDKSLGDKMKEALDKTEDKDKSK
                     GKKATKEKSGGKVSIKTFFQSLFTGRPSVKKKSAYAAKSKGKMTPLKFIKMQMYIIIG
                     LIVVILILVAAILIMKKKHDDYIAAQPDLGSKITQLTQEKHEQSALYQATVLQYEDRI
                     KRLEDTLNNSDSDTNKYKHQSEILLQQKRLYQGKYLSVSQKYDEALVVLNEIDTAKAE
                     FSDADYAMYNETVAQCKANIAISLYNSGNKLYQDLKYKEAYSNFYDIWQSYPDYDSVK
                     VWEKDNLYSNMVYDAVYKMSKCAFEIGEYNIAIKGYTFVEDSLSEFAKANREGLIYHS
                     AKAYAGIEDYVKAEELFKKVIDEYPASNLAIYAKDGLAAVRKKLGR"
     gene            29204..32227
                     /locus_tag="PLF14_02395"
     CDS             29204..32227
                     /locus_tag="PLF14_02395"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOT70180.1"
                     /translation="MNSIDLFAAIADTKIEQRKEYMEAKQPVPVPDVLFECVNIVKDN
                     DICKDNNEGKLIDKIKDKYSFPEEQSYEQQLKNYYEGLEQLKAQYAPFLKRRSKHAIK
                     RARLEFENFDFRFETREDIDDFSAVLEGRGIWESITIPHYHGPTGKWVTYYRTVFDYK
                     KDNLRDYIVFKGSDYITGVYVNGRHVLSHEGFFFAFEEDITEYLKEKGNVLVVRVEND
                     ITMLGENGRLGKKIYAATGIGWDDPKDGWHHCPPGGGIFDKVYIEKRESTFIFDACVY
                     PDIDNQTAKIKVVVKNVDRYKKDVKINISVEPFNFDDCFRYKGEFQRKIHEGENHYHF
                     DIKIENPRLWHSFTPYLYTVIIESDNSDQYEQNFGMRKFHMDTENKPYGTLYLNNTEI
                     LLRGANEMGHLQLCVMNDDYEQLIEDILIAKYCNMNYYRITQRPVQSEIYEYCDMLGM
                     MVQADFPLFGQLARSQFYEAVRQVGEMERHVRRHPSVIMVSYINEPTDQFKSDNQQIN
                     LNRAELENWFEICDRVIKQENPYRVIKRVEGDYDPPTFEGLSDFHCYNMWYTNHALPI
                     GDLYRGYLPAIRKDWKTACGEYGSEGLDNLDLMKERYPKEWLPKNEEDCWIPDRIVRA
                     QTNSMHGDWFYEQDNIKDWIYFSQLHQAKAASIMTLALRRRSDYVVQTALHLLIDAYP
                     SGWMKTVVGVDRVPKPAYFAFKEALIPLKVNLRCDRRTCYTNDVLNAECWILNDTAVD
                     YSDMTITAVLYDRHKNPLKTYKLNTSAKGCKSVPVGKIRIDTSDIAVKEREAFSLAVF
                     MEHQGKVIDTDVYNFDIFENNYESINAYPLGMLAKQVVNTSPAFKQADKETADIIIIS
                     DKKHLKEAEAIKKSINKPILFILNSCLENFKFNRHTYEMSDRRMWKATYCPINQSLLP
                     GYRWDDLSFLYDSDTDKINFVAEYFIQCDKVEKMYVFNYKKPAFGESAEGRKEKRPVL
                     MHNEDGIFTTLNLKGRVGVNPVSDKILYDVVRGLL"
     gene            32224..32928
                     /locus_tag="PLF14_02400"
     CDS             32224..32928
                     /locus_tag="PLF14_02400"
                     /inference="COORDINATES: protein motif:HMM:NF018964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1461 domain-containing protein"
                     /protein_id="HOT70181.1"
                     /translation="MRIAIRIMAVVFAIILCFVFLLSSIRYVAFNEKLYRKLQIKHEI
                     APFVLMTQEELDDITHEIIKYMKGDRKDLVIYDIQEGVKREIFNEREKTHMVDVLNLF
                     TVSEWVLVIMICLLAIIVIAGFLMDMDTMRSIFLKTVMITMVITVLLFIMLIFYMIVD
                     FHSFWMLFHEILFTNDLYLLDPYTDLLVNIMSLDFFVSISYYVSLCFGLLFLGMFATI
                     GGVKLMRKVIKSGQEV"
     gene            32912..34399
                     /locus_tag="PLF14_02405"
     CDS             32912..34399
                     /locus_tag="PLF14_02405"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOT70182.1"
                     /translation="MDRKFDRKIDITTIPGIRTYRNTLQLILLKVLNDIFPHRKVKIE
                     YSINKGSFCTVEDIKINNEHVEAIKKGVREYIDKDVPIKELHCTRQKAIEIFRERKIY
                     DIAESLEYVNNPKVLIYNLDGFISIFYSKVAERTSYVDLFDIRAYEPGFIYFYPTRHK
                     PYELPQYTEQNKLAAVFGEFNRWGDVLGIPDVTSLNRVVRSGSVNDIILATEALQDIK
                     LGQISREISEKHKKFVMIAGPSSSGKTTFANRLKIHLIVHGVKAKIISLDNYYINRAD
                     MVPDETGKVDLESVDALDICMFQDNMKELSKKGHTMIPVFDFNTASRKEELIPLSLSD
                     SEVLIIEGIHGLNPVMTKGIEEKYIYKIYISALTTINIDSHNRFATTDTRLIRRLTRD
                     YEYRNSSASRTLNMWESVRSGEDKYIFPFQEQADVIFNSALMYEHGLLRVFAEPILKK
                     VKKDDPAYDEAKRLLDILSAFEKIDSAAVPPSSILREFIGGSCFH"
     gene            complement(34400..34795)
                     /locus_tag="PLF14_02410"
     CDS             complement(34400..34795)
                     /locus_tag="PLF14_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOT70183.1"
                     /translation="MKNRLDLKKKIIKVEGCPQAIGPYSQAVRADNLLFVSGNLPVDP
                     ATGKIPEGVEKQTHTVLGNIATLLKGAGLDMSDIIKTTLFIKDMNDFSVINAIYAEYF
                     TSDYPARSTVEVARLPKDALIEIECIAKY"
     gene            34882..35538
                     /locus_tag="PLF14_02415"
     CDS             34882..35538
                     /locus_tag="PLF14_02415"
                     /inference="COORDINATES: protein motif:HMM:NF025059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOT70184.1"
                     /translation="MDILSDIKHKDILQVSFGKATADMSKAGIRRVNDGFQLEYFSDN
                     KAFHINFDGSNLFEHIYKLITTNFKECTIFTKQNDIVVFRNRKNELIIKNHKPTKSVV
                     NTSHDKQKNHIIKDGTDAPFLYYLGISDINGVVTDKGRRKFVQINSFLQIFDHAFEKL
                     SFDNKQLKVVDFCCGKGYLTFALHFYLHNIKHMNTNITGIDLKSDVIVILMILLKNTV
                     "
     gene            35535..35981
                     /locus_tag="PLF14_02420"
     CDS             35535..35981
                     /locus_tag="PLF14_02420"
                     /inference="COORDINATES: protein motif:HMM:NF025059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOT70185.1"
                     /translation="MTDLTFISGDIKAYDDYLDIAVGLHACDIATDIFLSSAVRNKAK
                     LIISVPCCQHQLFKQIDNEQLKPMLSYGLQKDRFTEMLTNTLRVLALKSKGYSTDMVE
                     FAPVEHTMKNVLIRAIYTGKPDENAKEEYNRLKTMFSVTDFEGDTI"
     gene            35978..36760
                     /locus_tag="PLF14_02425"
     CDS             35978..36760
                     /locus_tag="PLF14_02425"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HOT70186.1"
                     /translation="MRCLINCFSPTRNTYNLCEDMAKTLRNNGYAVDIFDVTRRAIRE
                     RRQAVDIAYDLFVIAFPVYNQSIPDAYAEYLKHLKINAENGAIVATYGSVTIGNALSQ
                     ANHILKEKGISIYGAAAIPAEHSYSSAFKDYGIYYDYSVELENFLKKVIYNVKNKKHV
                     DIKGQFAVAELFGQKFLGKVSIKIPRVDKNKCVRCNKCITACPTDAIDKDLSISVKQC
                     IRCAACVKVCEFGARLCTFNNPIAKSIVSKGVKNLGKLIDIT"
     gene            complement(36753..38177)
                     /locus_tag="PLF14_02430"
     CDS             complement(36753..38177)
                     /locus_tag="PLF14_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70187.1"
                     /translation="MIPVLENRWILKDNILKYYGLRKKPYTFKNTVNISASTAKIIQS
                     FDGIRHLSECKINSQIKQLIKKKIIVDVHSKRTVPDTIDNAFFCKNCCSNDYVIPGIE
                     FDDNGLCPVCTNMPSFKNIKDVLPVVNTIEHNEKAKYDVALFYTGGKDSTFLLYYFAD
                     VLKLKVLALTWDIPFMSDNAIKSMNNAKEIFTDTDFISKALNDEEQAAIYKKSYELMK
                     NVCICPFAAYPLFLKELRDSGVKYVVSGNEPAQPINLIFNNLSPKTFYNPLFQMLFRV
                     IYNMVRIFKGLKPLKKGRMEMLLLLETLAFGQPRFISAKTVRNPMIRNIHQALKEADV
                     LITSFRKEVEQASLDGNIPALIHIDFNDVIKGCMNNILSDGYVWKDIKALLKDKAGWI
                     DSENTDSGLHTSCKIERCKDFSQYQSFKAMESDIIPFSALELSVAVNLGSLTREDAIK
                     EIRHNSGFTDVAPCETGIMTKSFM"
     gene            38293..39273
                     /locus_tag="PLF14_02435"
     CDS             38293..39273
                     /locus_tag="PLF14_02435"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT70188.1"
                     /translation="MMNSKEVVKATLQFKYPDRLAVDFPPEYGTDFKFINMRANPDYR
                     PSKGKDEWNAVWDNIGISKLGEVKEFPLKSWDDFESMHIPDVTEDRRWEGVKENIENH
                     KDKFTMGVGISIYERVHFLRGLENTWIDIYENTDYLCQLIDILTDMNVHIVERFASYG
                     VDSLIFADDWGLQNSLMISPDKWREIWKPRYKRIYKAAHDNNMYTILHSCGDIRSILD
                     DLIDAGLDCIQMDQQQNMGLEYLGKYKDRITFFCPVDIQNIFVKGSLDEIRRYCHTMF
                     HALGSKKGGFMAKWYSDPVGAGHRREAIDVMCQEFLKISADVFGHSIVKY"
     gene            39390..39830
                     /locus_tag="PLF14_02440"
     CDS             39390..39830
                     /locus_tag="PLF14_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOT70189.1"
                     /translation="MQIEIKIDEDCKEPKIIVVTDKMTEEINEVIKKLSEQQPQIIAG
                     FKEEIVKVLELSDIYRIYASGGKVFAETTHGEYTLRLRLYEIEERLDNNNFVRISNSD
                     IINLKKVKGFDLSLAGTICVILSNGTVTYVSRRFVARIKQLLGI"
     gene            39840..40310
                     /locus_tag="PLF14_02445"
     CDS             39840..40310
                     /locus_tag="PLF14_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HOT70190.1"
                     /translation="MKKKIINRGLLGFPLGIAIGYIVSILVSLYAGDGMFHFAKPELV
                     EKVGSELNAVILQTVLSGILGAGFAMSSVIWEIETWSLAKQTGIYFAVICLVMFPISY
                     VAKWMPNTVGGVISYIAIFLGIFIVTWLIQYFIWKSKIKKINEKVKDVEKLSTE"
     gene            complement(40409..41626)
                     /locus_tag="PLF14_02450"
     CDS             complement(40409..41626)
                     /locus_tag="PLF14_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70191.1"
                     /translation="MCDTMWKSFEKGGIFAKNSDRSCNEINLVQFIPGGKTGGQPVKC
                     TYITVPQEEKKYSVLLVRPHWMWGAEMGINECGVAIGNEALFTKSKGKRTERLLGMDI
                     LRIALERAKNAKEGIEEIKKALTAFGQGGNCTFDKTFYYDNSFLVADKKEAYILESSG
                     LEYKTKKLEGYGNISNRITIEEDDFAKKHTNNLITHFAGSMKRQNCACNAIKDAKGIR
                     DVFGALRAHDTDDRHKLYTKGSVSSVCMHQSFLGDHTTGSMVVDFREDIPVIWVTGSS
                     TPCLSVFKPVFFGHINPPVFLDEKDSLNYWLKREYLNRAIFAGLTNADSHIKQVKEIE
                     EDFISGYESLLKKGATVEQFADFSKKCSEKEETFINSYSKIIETVKNRTVQLPGMWGK
                     KTKRLIEQNHMKY"
     gene            complement(41799..43094)
                     /locus_tag="PLF14_02455"
     CDS             complement(41799..43094)
                     /locus_tag="PLF14_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT70192.1"
                     /translation="MKRMIEDKLNKWKVSGSRKPLLLMGARQVGKTYSCLEFGKNNYQ
                     NVVYLNFEGDMRLLHDIFREDLEPARIVQELSYYSKQAIIPEKTLIIFDEIQACEMAL
                     NSLKYFCENAPQYNIIAAASLLGIAINRGKYSFPVGKVDMVTMYPMSFDEFLYACGKD
                     LLLEKIKESFSSFKPLSAVYHEEALSLYHSYLVIGGYPAVVKTYLEKNDFNVVKAQQA
                     NIANAYIADMAKYSTPNITIKSTDIFNTLPAQLAKENTKFRYNIIKSSARAKDYELSL
                     QWLKAANVVLESIKATEAKHPLSVYQEIDSFKIYYSDVGLLSYKAGIQPQDVISNISS
                     HRARGMLAENYVAQQLVAAGYPLNYWQSSGTAEIDFIIRIDDSIIPIEVKSADNVRSR
                     SLSVFADKYNPDYAIRFSTKNFGYENSIKSIPLYAVFCL"
     gene            complement(43310..43395)
                     /locus_tag="PLF14_02460"
     tRNA            complement(43310..43395)
                     /locus_tag="PLF14_02460"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(43360..43362),aa:Leu,seq:caa)
     gene            43504..44937
                     /locus_tag="PLF14_02465"
     CDS             43504..44937
                     /locus_tag="PLF14_02465"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007494030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOT70193.1"
                     /translation="MNKDIVNNYTLLCDYYELTMANGYYIAGYKDKYCYFDMFYRNNP
                     DNGGFAIACGLDQVIEYIQNLEFTDEDIAFLRSKKCFDEGFLKYLKGFKFTGDIYAVP
                     EGTVVFPNEPLITVRAKTIEAQILETFILLTVNHQSLIATKSNRIVRSAKGRPIMEFG
                     ARRAQGTAASVLGARAAYIGGCAGTSCTLADKMYGVPAGGTMAHSWVQMFDTEYEAFY
                     IYAKTYPDNCVLLVDTYNVLKSGVPNAIKVFKELGIKKCAIRLDSGDITYLSKKARAM
                     LDEAGMTECKITASNSLDEYIIRDVIHQGATLDSFGVGERLITSESDPVFGGVYKLVA
                     VEDDKGNVIPKIKISENPTKITTPHFKKVYRIYCKDMLKAEADLICVYDEVIDTSKPL
                     TIFDPDYTWKRKTFEDFTIKELLVPVFKNGECVYDRPSIEEIRAYCAKQIDEQWDEVK
                     RFENPHNYYVDLSQKLWDIKYRMLNRE"
     gene            44972..45358
                     /locus_tag="PLF14_02470"
     CDS             44972..45358
                     /locus_tag="PLF14_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003146921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70194.1"
                     /translation="MLLLGVTEDKGWLAATFNLTYRVGWEQIQKGVGTAYRFFKKTEI
                     LVDDKPVSVTSGEDIMKLEEAGSMTIRGLSTIIKVPLMITFYNQLQTVNVALPAQNEE
                     FRNTDYQKFNMSLGQYMDSIELAMYR"
     gene            45454..46929
                     /locus_tag="PLF14_02475"
     CDS             45454..46929
                     /locus_tag="PLF14_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013659075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOT70195.1"
                     /translation="MIKIIIIAIFIAIQVAVGIYSVRKIKNIDDFLLGGRKMGAWLSA
                     LAYGTSYFSAVIFIGYAGGIGWEMGTSAVWIGIGNAVAGSYLAWRVLAKRTRSITREL
                     DAKTMPEFFQKRYDSEALKIFTSVLTFIFLVPYTASVYKGLGYLFSKLFPASVSVSQA
                     FTITVIIMAAFTLIYLVLGGYIATALNNLIQGTIMIIGTVIIVVYVVKSPEVNGFANG
                     LASLNNIKEGLGTITGPDPWKLLSLVLITSFGVWGLPQMVHKFYAIKDESSIKKGTII
                     ATVFAAVIGIGAYLTGSFGRLFFSEIPNGSVDTIVPNMLVKILPDAFLGIFMVLILAA
                     SMSTLSSLVLVSASSISVDLVKGYIKKDMSEKSQLTLFRILCGVFVVISVIMALMNNN
                     AIVTLMNLSWGTLAGCFLGPYVVGIYSRRADKVSAFAGMLSGFTVMMTLVIIKGMTYS
                     TVAGCIAMATSVAVSFIVSRLSWKKNITVEEILNEKVVTGE"
     gene            46904..48646
                     /gene="iorA"
                     /locus_tag="PLF14_02480"
     CDS             46904..48646
                     /gene="iorA"
                     /locus_tag="PLF14_02480"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HOT70196.1"
                     /translation="MKKLLLANEAVARGLYEAGVKVVSAYPGTPSTEITEATSKYDEI
                     YTEWAPNEKVALEVAASSAIAGARSFCAMKHVGLNVASDPLFTTSYIGVNAGFVIAVA
                     DDPGMHSSQNEQDSRHYAKAAKVMMLEPSDSQEAKDFTKKAFELSERFDTPILLRLST
                     RVSHSRSLTTLEDRVDVPLKDYVKNPKKNVMMPAMAKVRHEIVEERVIKQKEYAFLSE
                     LNKICIKNKDIGFISSGIAFQYAREALPDASYLKLGITYPISEELLKKFASSVKKIYV
                     IEELEPIIETACKACGIEVTGKQLLPVTGEYSVELLRNRLFGQKPYNCMEGYDMSKVP
                     GRPPVMCPGCPHRGIFHILKRLKLTVTGDIGCYTLATLQPLVAMDTSLCMGSGITSLH
                     GFNKARGDEYMKKCVGVIGDSTFVHSGITGLIDIVYNKGVGTVIVLDNSITGMTGHQN
                     NPLNGLDIHNNPAKQIDLVMLAKSIGIERVRIIDPFDVEEAMRVIKEEVNAMEPSLII
                     SQRPCALLKTVKYKGCVTVDRDKCTKCKMCMSLGCPAISFKNNKIEIDTSLCNGCGLC
                     VNVCRFGALKKEEK"
     gene            48646..49221
                     /locus_tag="PLF14_02485"
     CDS             48646..49221
                     /locus_tag="PLF14_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOT70197.1"
                     /translation="MVKSIVIAGVGGQGTLFASTIIGEAATLKGFDVKVSEVHGMAQR
                     GGSVITYVRYGEEVISPIIPVGEADVLIAFEQLEALRWAHYLKKGGILISNTQKIDPM
                     PVITSSMQYPDDIISELTGRVDNCIFMDASVLAEQSGSIRTVNSVLIGRMARDSDIEK
                     EIWLEALKKIAKPAFIDMNIKAFEAGYNYTV"
     gene            49312..51429
                     /locus_tag="PLF14_02490"
     CDS             49312..51429
                     /locus_tag="PLF14_02490"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT70198.1"
                     /translation="MSITEKDKIFEQDRLNEVYAKLSKRIEDLEVSLKQSIDSLRKSN
                     QMMWESGKRNPADFHDAIENMPYLESIKYDFFKTETIKKEVRRMYLLQRSMYFGRIDF
                     QEQNEEDVENIYIGKFSLTDDEGQYLVFDWRANICSLFYENELGNVYYDAPDGRIYGE
                     MSKKRQFDILYNNIHAMFDSSLMIDDDILKDILSKSKDSKMSNIVETIQKEQNKAIRS
                     TYSTIIVDGPAGCGKTSIAMHRAAWLMYKYKNELQNNQIIIFSPNETFNDYISEVLPE
                     LGEENIRMSTFRNLAYHILSDRRVLISKYVELEDVLLDNEYIDIDVLRLKYSVDFANK
                     LKSYTELISEGDFPFKDLIVDGITIFTKREFEDLFYNEYSFNDVMTRLAKIRIVLNER
                     IFPVIRNLRLEYVRKVKADELDMSKGFKELRDSCKRIYQAVDMSVSADFEMIYAQFLA
                     HSYSAKISSDFLKRLRDGYIYAEDTAPLIYLRLLLGNKYNFSDIRHVIIDEFQDYSYI
                     EKLVISLLFGKCHMTLLGDINQKINHYQEKVNDKDIFKGARSVKLDKSYRSTYQIAKF
                     CNDMLKDNLDIKYFNRQGKQPEILNLNTADLDVIANTVINKVSKYRASGHRSLAVITR
                     TEAFAKKLQPYLKDAKINRVTEKSTTYTYGAVLIPSYLTKGLEFDAVIVLDTQNDRFK
                     KEEELHLFYTCCTRALHELTVIN"
     gene            complement(51431..52342)
                     /locus_tag="PLF14_02495"
     CDS             complement(51431..52342)
                     /locus_tag="PLF14_02495"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT70199.1"
                     /translation="MNKDYFSIIGKSGAFFVFKPHITDNPDFILVVAPGLAEHAARYA
                     RLSEFMKNHNVQLYCIDHIGHGVYTESLGIWPVDGFFECVSNMNSLVEYVRKQHPDKK
                     VILFGHSMGSFMTLAYAERYASNIDGCILSGTNDQQPSLVLGAGRFIAALQELFIGRN
                     KPSGILNALSFGAYNSQFKPNRTEFDWLSRNESEVDKYVEDPLCGYVPSVGMFKELFK
                     GLSVIYKKESIAEIPKELPIHSFCGSDDPVGGNSKGPLALVARLQNAGIKNVTSRIFE
                     DMRHECLNELGNDAVMKHVYEVCKDMI"
     gene            52499..53794
                     /locus_tag="PLF14_02500"
     CDS             52499..53794
                     /locus_tag="PLF14_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012068258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOT70200.1"
                     /translation="MIRDRVESISLDEMRTIQGKRLVETVKHVYDNVAYYRRKMESIG
                     LLPGDIRGYEDLHKLPFTTKADLIETYPYGMVAVDMDKIVRVHASSGTTGKRIVVAYT
                     KEDIDTWADVMARCFARIGTDVNDVVQVAYGYGLFTGGLGTHYGAERLGATVIPISGG
                     NSRRQVEFLIDLKPTVIACTPSYMLHLYEVMTEAGVTVDDISLKAGLFGAEPWSENMR
                     KEIERLFNIKAYDIYGLSEITGPGVASECCAQNGLHINEDCYLPEIVDTETQQPLGLG
                     MQGEIVFTTIKKKGMPLIRYRTKDITKLFRDDCRCKRTLVKMERVMGRTDDMLIIRGV
                     NVFPSQIESVLLEFSSVTPNYQIVVDRINMMDTLEVRVEMNENLFSDEVRKIEMLERN
                     ITGAIESVLGISAKVKLAEPKSIERSEGKAVRIIDRRKI"
     gene            53805..54221
                     /locus_tag="PLF14_02505"
     CDS             53805..54221
                     /locus_tag="PLF14_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOT70201.1"
                     /translation="MLVKQISIFLENKEGRLAEVTSILARANININALTIADTADFGI
                     LRIIVDDAEKTEKLLREYGFSVSVTDVLAIDIDNVPGGIARPLIMLHKNGISVEYIYA
                     FLAKEDDTAHVIFKVNDIETAVRILTKNGFRNTAKL"
     gene            54218..55486
                     /locus_tag="PLF14_02510"
     CDS             54218..55486
                     /locus_tag="PLF14_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HOT70202.1"
                     /translation="MKAEIIAVGTELLMGQIDNTDASYMSKRFPECGVYVYYHTVVGD
                     NRGRLAECINIALKRADVVVLTGGLGPTQDDITKETVAEVLGLKLVFHPEILENVKEY
                     FNTIRHNRKINIMAASNEKLAYYPEGCLILPNNNGTAPGCIISHFVEGADKTIILLPG
                     PPWELQPMFDNYVVDYFKSKIQEGLKSVYLRMFGIGESAMESVISDIVSTQTNPTIAP
                     YAKKGEVMLRVTARYEKGKQDPDLILKPILDILHERLGEYIYSDKDVELKVVTANLLM
                     SKNITVSFAESCTGGMLSELLTDVPGISKVFLGSVVAYSNEIKQSVLKVSGNTLKKYG
                     AVSEQTAKEMAQGVRLLYNTEVGISITGIAGPSGAVADKPVGRVYIGISTEQETLSYE
                     YTFSGDRDKVRYFSCLNALNILRLYALSKG"
     gene            55490..57100
                     /gene="murJ"
                     /locus_tag="PLF14_02515"
     CDS             55490..57100
                     /gene="murJ"
                     /locus_tag="PLF14_02515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOT70203.1"
                     /translation="MKEIRFKSTVFLIMASVLLSRIMGFVRTALIPVKMGGLSRVTDA
                     YNAAFQVPDLMYSMLIGGAIASSLIPVLAGYVAKGEEKKGWRSVSSFMNLVSIIMFVV
                     VILCVIFAKPLVNMLVPGYDAETLELTVQLTKILMPIAFFMMLAGLCNGIMNSYNEFI
                     IPAFGPCLYNLACIISIALFSNDNVADNYGVKKVVLGIVICAGLYFLFQFAFTFKHFK
                     NNYTTVIAYKEPDFKLLIALAIPSLLTSMLGQISLIISRYFTAFFDVGSVTALELVNK
                     TWQMPLGIIAQAIGIAMLPSLAAVYDADKKSEVVYKINTSLRLVIFLAIPSAVGLSVL
                     SKPLIQVLFNYGTIEQEGITLAANLLIGYSLALVMQCIITIVNRVYFSTKNSIIPFWG
                     SLIGVVFNYLIAYLLLKLTDAGIFSVPVAYSLSSVLNLVFLGLLFKTRIKGYTFRDNI
                     PTILKSLMSAVMMGAAIYLLDISLMPAIFGTAIIQLGKIKQFLWILIEIGIGAGIYML
                     LTYLMKADQARELIDMVRAKFKSIIGNN"
     gene            57185..58207
                     /gene="recA"
                     /locus_tag="PLF14_02520"
     CDS             57185..58207
                     /gene="recA"
                     /locus_tag="PLF14_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOT70204.1"
                     /translation="MMDKKKALEASLAGIEKAYGKGAVMRLGQNTAMTVESIPTGAIS
                     LDIALGIGGVPRGRIIEIYGPESSGKTTVALHIIAEAQKGGGEAAFIDAEHALDPIYA
                     KKLGVNIDELIVSQPDTGEQALEICEALVRSGALDVIVIDSVAALVPKAEIDGEMGDA
                     HVGLQARLMSQALRKLTAVVAKSKTTTIFINQLREKVGIMFGNPEVTPGGRALKFYSS
                     VRIEVRKADNIKLGDNIIGSKTRAKIAKNKIAPPFKTAEFDIIYGHGISKEGCLIDVG
                     VDCGVVDKAGSWYSYKDQRIGQGRENAKDYLVQNPKIAEEIEKLIRAAFEAHKTPEQP
                     TEAADE"
     gene            58200..58679
                     /locus_tag="PLF14_02525"
     CDS             58200..58679
                     /locus_tag="PLF14_02525"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HOT70205.1"
                     /translation="MNDELSIVKREVLKKDYILTLSDNTQLFIDEETYFKYCIYDKET
                     LSNAFIEDIKDKTEAMQCYKKAVAYLLNGKKTENRMRLYLENKGFGPKAVDSCIHRLI
                     EEGKIDDVAFMDKFIKANLKKDTKREKLIAKLIYHGIDEQLAVQEVDKAVGYEEDTY"
     gene            58660..59982
                     /gene="rimO"
                     /locus_tag="PLF14_02530"
     CDS             58660..59982
                     /gene="rimO"
                     /locus_tag="PLF14_02530"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOT70206.1"
                     /translation="MKKILINSLGCPKNLVDSEIMAKSLKDKGFVLTDNIEDANIAII
                     NTCSFIQPAVEEAISVILEAAEYKKRGILEKLIVTGCLVERYKEEIITSIPEVDVCVG
                     IDGYKDIASVVNNTDEVLICGNKTDVTFMNKERMLSENTASTYVKIAEGCDNRCTYCT
                     IPSIRGRYRSRSIEDIYAEAEKLCSLHGIKEITLVAQDTTAYGTDIYGKPSLDLLLKK
                     LETVKSLQWIRLLYCYPELITDDLINSVKNSKKILHYLDIPLQHSSDRILKLMGRKGT
                     CAMYTELIDKLRFHIPDIVLRTTFIVGFPQETEEDFENLCEFIKASRFDRAGFFEYCR
                     EEGTPAYRLKGQIPKKVKIQRRKQAEQIQQDILISKNDERIGKTYSIIIQGISDDGLF
                     YVGRSYAEAYEIDPVIYVAAKEELTVGDITDAVIVAKHEDSLIAEVVQ"
     gene            59979..60542
                     /gene="pgsA"
                     /locus_tag="PLF14_02535"
     CDS             59979..60542
                     /gene="pgsA"
                     /locus_tag="PLF14_02535"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003376978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOT70207.1"
                     /translation="MNIANKLTLIRIILIPFFIIFAVPTPEWLSEPVFSFHQQYGTLI
                     ALVIFVIASLTDLFDGYLARKYKLITNMGKFLDPIADKILITSALVVLVERGQIAAYT
                     VVIIIARDLIVDGLRLVVSEKGEVIAANVFGKIKTNLQIISVCLYLLAFYINLWYIPK
                     IVYALALIATVYSGVIYVKESIKFIKD"
     gene            60667..61203
                     /locus_tag="PLF14_02540"
     CDS             60667..61203
                     /locus_tag="PLF14_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013403463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis transcriptional
                     regulator"
                     /protein_id="HOT70208.1"
                     /translation="MTKATLKKLKKLEDLQILLSESPYMNDKQLAEALGVSVPCIRNY
                     RNELGIKQVRERIKDTVRSLAPEDIVGTITNLKLNEGASAEFVTTADMVFKKTNIVRG
                     QYLYSFAESVAISVIDAEASLVGVANIKYKEPVYANEKLTAQAKVTQIRLNNYFVWVF
                     VYREDVEIFRSKFILVSV"
     gene            61215..62201
                     /gene="plsX"
                     /locus_tag="PLF14_02545"
     CDS             61215..62201
                     /gene="plsX"
                     /locus_tag="PLF14_02545"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOT70209.1"
                     /translation="MNILVDAMGGDNAPEAIVIGCLNAIKTEEGFDITLFGDSKIIAD
                     ILCNYNYDKSRIKVEHCSEVILFKDTPTVAVKNKKDSSLVRALTAMREGNGDAVISAG
                     NTGALLTGSLLITGRMQKVIRPALGALIPSGEKFALLIDAGLNSNLRPESYVQFAKFG
                     SEYMKIVTKCDNPLIGLLNIGTEDTKGSAEVKEANLLLKQSGLNYYGSIEGNSIVEGV
                     VDVVVTDGFTGNVSLKVLEGTASYIFSKMKSVFSMDVFHKLAASVIRPDMKKFKDNLN
                     PDIMGGAPILGVNGLILKSHGSSNAKTIMHVVLKAVKLAKNDIVGNIRRAVC"
     gene            62298..62525
                     /gene="acpP"
                     /locus_tag="PLF14_02550"
     CDS             62298..62525
                     /gene="acpP"
                     /locus_tag="PLF14_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOT70210.1"
                     /translation="MIFEKVKAIIVEQLGVEADDVTMESSFVEDLGADSLDIVELIMS
                     MEDQFKITIPDTEAENITTVGSAVKYIESNR"
     gene            62648..63358
                     /gene="rnc"
                     /locus_tag="PLF14_02555"
     CDS             62648..63358
                     /gene="rnc"
                     /locus_tag="PLF14_02555"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOT70211.1"
                     /translation="MEKVIDKSIASVEKAIGYSFKDKSLLSTALKHSSYDKTENYEKL
                     EFLGDSVLSLVISDFLFRTHNYLQEGEMTVTRAYAVCEESLSKVSKRLGIDKYILIGS
                     SAVSSSVNTNKSVIADAFESLIGAMYLDSGFKYVRPFIISNLNEFIVEYIRSGDNNDH
                     KTKLQHFVQSRYQEEPKYLVKGEKGPPHDKTFLVEVIITGKPYGKGIGKTKKEAQQAA
                     AKDALENIEKKGLDNYDS"
     gene            63348..66896
                     /gene="smc"
                     /locus_tag="PLF14_02560"
     CDS             63348..66896
                     /gene="smc"
                     /locus_tag="PLF14_02560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOT70212.1"
                     /translation="MILKKVELQGFKSFATRTVLEFNKGITAIVGPNGSGKSNIADAI
                     RWVLGSQSSKQLRGGNMSDMIFSGTQYRRPVGFAEVSLYFDNSDKHLAVDFEDVKITR
                     RLYRSGESDYSVNDKSCRLKDIQMMFFDTGVGKEGYSIIGQNEIKDIVDSNSLERRGI
                     FEEAAGIMKYKQKKLEALRKLSNTEENLVRITDIISEIENQLEPLRLQAEKAKEYLRV
                     YEILKVNEVGLYLNNLQMYQKRIQKYEQDIQVLSEDILTRESELENIKQENRDSINII
                     NRYEEQEQNLKDELNTFLRDLESQRHEIILCNERLSNADSELIKNGKEIEEYQLRENE
                     LSKDYLTKEDRRKYLENQYTSYKEKLDLLTAQAESITASLSGSEKILEGVKASLMRHI
                     EETSDSKLVLNNVINEKNALKTQLDMMDNDINKKVLENDSILLAKQDNQSKNIAAKKQ
                     QEQLASRRDKSKTEYALLQKRMEQAESAERNLKTNIEILQSNYKLIYDMEKQMHGYKQ
                     DVKAVLENKSLNGIVDVVAKLMGTEEIYNTAIEAALGGNIQNVVVEDEEAAKAAIAFL
                     KENKLGRVTFLPVSSVRPNQFNKDEEREILSYKGVIDVAYKLLEYDSKYENIAKNLLG
                     RILVVDTYDNAVLIARKTQSRYKLVTLTGEFFNTGGSITGGTQNVGKVGLLGRHKQLD
                     RLESEIAAHKEKLEQSVTQSRSMSKQASELASDIDKTEQCIIEGNMLTAKLESAISAL
                     DEAYKSNTDALNATKNRKKELTDKYHGYDDKIAILNNQIDALSEQIEKDKLKINEYEE
                     GHKEQSAKRDNIYLDISDMKVSVNSIEESLKNNDESLIRIGFEKQALTVKRNELIKRN
                     EQLKEIKADLQDKIQTKSKNISNPEKVEKEKNKAIQELQNKRKKLSQENEKYIQNITD
                     VNRDIMLLSNKISSIEVNKAKTDAEMTAMKERMWDEYNLTHNEALQRHGEIENITEAR
                     KEIAEARNIIKQLGPVNIHSIEDYAKTNERYRFFTTQKEDIEKSKEDLYKTVNEMNTV
                     MRKIFKEKFEIINDNFNEIFARLFEGGQAKLELTDQSDILECGIDIIAQPPGKKLQNM
                     MLLSGGEKAFTAIALVFAMLKLNPSPFCVFDEIDTSLDDNNVLKYCKYLNEHKSNTQF
                     VLITHRKGTMENADTIYGVTMQEHGITKVVSMKITE"
     gene            66898..67827
                     /gene="ftsY"
                     /locus_tag="PLF14_02565"
     CDS             66898..67827
                     /gene="ftsY"
                     /locus_tag="PLF14_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013411935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOT70213.1"
                     /translation="MSFFRRLKEGLSATKEKLTKQIDKLLASFRKIDEELFEQLEEVL
                     IESDIAIDTVMQITEQLKQEVKINNISDPKQIRDLLKKKLMDMITKNSNTTLNALKDD
                     YKDKLNIILVVGVNGTGKTTSIGKLAGMLTGHNKKVLLAAADTFRAAASEQLIEWAKR
                     TGVDIVAHKSGADPGAVVYDAIHASVKRGADVLICDTAGRLHTKSNLMEELKKISRVI
                     GKEAPDAIVETLLVIDASAGQNSLVQARVFNEYVKIDGIILTKLDGTAKGGMVISICN
                     ELDIPVKYIGIGEKADDLRPFDGKSFIEALFDN"
     misc_binding    67851..68058
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            68104..71232
                     /gene="ileS"
                     /locus_tag="PLF14_02570"
     CDS             68104..71232
                     /gene="ileS"
                     /locus_tag="PLF14_02570"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOT70214.1"
                     /translation="MYKKVSTDLNFVKRELETLEFWKQNRVFEKSIENREGCEHFTFY
                     DGPPTANGKPHIGHILTRVIKDIIPRYKTMKGYKVLRKAGWDTHGLPVELEVEKMLGL
                     DGKDDIERYGIEPFIKKCKESVWKYKGEWEKMSDRVGFWADMENPYITYDNNYIESEW
                     WALKKIWDMGLLYKGHKVVPYCPRCGTSLSSHEVAQGYKDITDKTIYVKFKVKDTADT
                     YFMAWTTTPWTLPSNVALTVGAKFDYAKVKYNNEYIIVAKQLVPSVFEGSDYEIVSTV
                     KGKELEYMKYEPLFDFVDEDAKKEGFYVCLGDFVTLTDGTGIVHTAPAFGEDDSIIGK
                     KYNLPFLQMVDEAGHMVEQTGFIKGLFVKDADKIIIGDLDKRGLLFKSSEIVHSYPFC
                     WRCDTPLIYYARSTWFIKMTAIRDSLLKNNDKINWMPATIKDGRFGNFLRAVVDWGIS
                     RERYWGTPLPIWECECGHREAIGSIAELKEKAVDCPKDIELHKPYIDNVHLKCPKCSA
                     MMKRVAEVIDCWFDSGSMPFAQHHYPFENKQVFEQGFPADFISEALDQTRGWFYTLHA
                     ISCLLFDSPAYKNCIVLGLVQDKEGQKMSKHKGNVVDPWDVLENQGADAVRWYFYTVS
                     APWLPSRFHDKAVTEGQRKFMGTLWNTYAFYVLYAEIDKFNPLKYRMKKDTLCLMDRW
                     ILSKLNTLVKNTDKNLVNYRIFEASREIQEFTDQLSNWYVRRSRERYWGSEETQDKIN
                     AYLTLYTALVTLAKLAAPFTPFITEQIYGNLVVNLDKSAPISVHLTDYPVCDESLIDK
                     EMENQMEELLKVIVVARSLRNISGIKNRQPLRNLYISSEKKLNQEYIELLKDELNIKD
                     ITYVTDASMFIDYSLKPQLRTLGKKYGRLLPAISEHLKSADGAHVMGLFEKGLNYEFT
                     VEGNAVVLEKDDVLSETKEKDGLISQSDNGITVVLDINLDEQLIEEGYVREIVSKIQT
                     MRKDAGYDVTDKITVYIESSDMLEKLVVKNFPSIMKDVLATGIAKAADDSFYCATWDI
                     NGVQADIKIKRV"
     gene            71332..72402
                     /locus_tag="PLF14_02575"
     CDS             71332..72402
                     /locus_tag="PLF14_02575"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT70215.1"
                     /translation="MKQLKVAIIGQGRSGYSIHGTYLIDDTERFKVIAVIDFIEKRRL
                     LAKERYGSHVVTSDNYKDLFGMDIDLVINSSYSHLHYSITKDLLMHGFNVLTEKPFCR
                     TADEVQDLIDTAKKQKRMLAVFQNSRFTAYFMKIKEILDSKVLGDIIQISIDFSGYAR
                     RWDWQTLQSYNAGNLYNTGPHPVDQALQLYGEGMPEVKAFMRRVNTFGDAEDYVKLIL
                     YGENKPLIDVEVTSCQGYKPYVYNIQGSKGALTGTMTQINWKYFKTEELPEQKLLLSF
                     DKEGTDIPAYCSENIKWYEEQWTSEGEKYGTFGASTRRYYDMLYNHMVNGGELTVTAE
                     QVKKQIAVIEEAHRQNPLSKKF"
     gene            72412..74376
                     /locus_tag="PLF14_02580"
     CDS             72412..74376
                     /locus_tag="PLF14_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70216.1"
                     /translation="MKETENKETIKSDEVQQVSDTDTVEHIAEEDINTEEDNITEEVS
                     EKRNVKKSKFSQHLKKFGFYYGLGLLIVILAVVLIFDGNRRLRGDIPYDDNIYIVGDN
                     SISMIRYNQEPVKITSQLFRNYEDQKLGLEAHNGILMSKDGNSIMYVDDLYLLSNEDL
                     SLTKLVGTLWMYNGVKNIKIADDADFYYVVNDDFNIVLYKTYRLAEDESRLISNLYKF
                     DLATGQSVILREDIDTDLYSVSNDGKVCVFLDEKQESFVENGEPTYSLFVYKDGQTEK
                     LADSVYDLGASVYTGTAEVNYPIISKNGDKIVYAVVDYIYMVEDEQEEQPEDVEETQT
                     EEEEKKVVYPTFNMYLYHDGVSKLIAKSCAQLLISEDFSQMIYIDAVVEHLAAYDEIG
                     FCGTYNHYDVETGKSTKVMDNVFGFVERNVLGFVDNDFIDANFYFKQYDIVMNTAHLY
                     TIRNGKEILIDDYASVGEDEDLNIKLVFFSPDYKDVYVFKEYVSKTSSLLHRFTIKSN
                     GSISDYMCEEMQVISAIALSKDGDYLVYNADGKLIIISDKNVKKTIEVNGVSSFGITE
                     DGKYVYYYKETEIGLGAVYYYDLYKSEKPVKFLNNVNEVWNYKDNKVMLRINTDYNTM
                     TGDLYLSDFEQFEYISPDVYAQITTKVTER"
     gene            74401..76041
                     /locus_tag="PLF14_02585"
     CDS             74401..76041
                     /locus_tag="PLF14_02585"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOT70217.1"
                     /translation="MPLGGLNEIGKNLTVFEYRNDILILDCGIKFPQEDMPGVDIVKP
                     DMSYLVENKDKIKGVVITHAHEDHIGAVCYLLKEINTPVYGAKLTLGLLESKLNEMNL
                     LTTTSLNIIDGDSIISIGCFKLEFIPVNHSIPDTYAVVINTPVGIILHTSDFKIDYNP
                     VNEKVTDLNRFSELGNRHVLLMMADSTNATKEGHSMSERAVGDSLDNLFRKASSRIIV
                     ASFASNIHRIQQILNLAEKYKKKVCIFGFSMRNNIRKSIELGYIQVKRGIIIEDFEIK
                     NYKHKDMVFIVTGSQGETNSALSKIAFKEHQKISVEKNDLVIISASVIPGNEKYLYRV
                     INELFKSGANVIYQALNEVHASGHAYRQELRLLLTLVRPKYYMPVHGEFMHMESNALL
                     AMEMGIKEKNIFLLENGDVMKVYPNRISVDDKDKIKVGLSFVDGHGVGELGDDVLKER
                     EILAGGGILNISIVLNKSDRKSAVEPVIIPVGFVYESESDDIIRKIREMLDEDIGKYT
                     KTSMSINELQNSVKKRLRKFLYQETKRNPVVLPVIMEV"
     gene            76050..77273
                     /gene="tyrS"
                     /locus_tag="PLF14_02590"
     CDS             76050..77273
                     /gene="tyrS"
                     /locus_tag="PLF14_02590"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOT70218.1"
                     /translation="MKAYDILKERGFIAQATDEEAIKNLLNNEKIVFYIGFDPTADSL
                     HIGHFLQLIVMSHLQKAGHKPIALLGGGTAMIGDPTGKTDMRRIMTREEIDYNASCFK
                     KQMSRFVDFTDDRAIMVDNYEWLSKLEYIPFLREIGMHFSVNRMLTAECFKSRLEKGL
                     SFIEFNYMLMQSYDFLLLRRKYGCILQLGGDDQWSNIISGVDLIRRKELGEAYGMTFN
                     LLTTNEGKKMGKTEKGAIWLDENKTSPFDFYQYWRNIDDAEVIECLKLLTFLPMEEIK
                     KLSKLKDAEINHAKEVLAYEVTKIVHGEKKADDAKIASRALFSNVGEIGNMPSKELTK
                     NNLEEGYTILDALTDAGFIKSNSEGRRLIQEKCVSVNDIVVEDFKLCINSDMFTDGEM
                     LLRRGKKKYFRFILK"
     gene            77349..77984
                     /locus_tag="PLF14_02595"
     CDS             77349..77984
                     /locus_tag="PLF14_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013977160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HOT70219.1"
                     /translation="MMIYDDISAALQKGRRKDVEALIQQAIDDKLPPKDILNKGLLAG
                     MDIVGEKFKNNELYVPDVLVAARAMNAGTALLKPLLAEAGVESIGKVVIGTVLGDLHD
                     IGKNLVKMMMEGKGFEVIDLGVNVSAEQFVQSAIEHDAEIIACSALLTTTMGEMANVV
                     KVATEKGIRDKVTIMIGGAPVNEAFKISVGADIYTPDAASAAEEAAKVVLN"
     gene            complement(78098..79336)
                     /locus_tag="PLF14_02600"
     CDS             complement(78098..79336)
                     /locus_tag="PLF14_02600"
                     /inference="COORDINATES: protein motif:HMM:NF017452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOT70220.1"
                     /translation="MNIVGIISEYNPFHNGHLHQITEIKKLFKNNIKVICLLSSDFVQ
                     RGEPALLDKYARAQMAISNGADMVLEYPTLFCCASAESYADNAVYILNNIGIIDYISY
                     GFKSSDNDNVKKISSLLAYESNEYKEILKAELSKGLSFPKARQHALIKLLNKSDTDIY
                     KLSETLKSSNDILAIEYEKALIRQKSDILTVPIERTGSDYNDPYMKENFSSATAIRKY
                     LLSEKVINNKDLADNLPPSTLKILEDYSEVKHFGSMYLFENLIFYSVISKSNDELKKY
                     PLVNEGIENLLKNNVFSCSSVNDFINTCVNKRYPATRIQRLLIHILLGMNKGITYKYK
                     RPGYIRVLACRNKSLLSLISSKSSLPVITSLKKSYDDFDSDLKTLIEFENKACDIYNI
                     VCLKDKSQYLNEFSVKPMFD"
     gene            complement(79358..80449)
                     /gene="ychF"
                     /locus_tag="PLF14_02605"
     CDS             complement(79358..80449)
                     /gene="ychF"
                     /locus_tag="PLF14_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOT70221.1"
                     /translation="MKLGIVGLPNVGKSTIFNVMTHNKAQTNNYMFSTLEPNVGIVNV
                     PDERVDFLASIYKPDKTTYATIEFVDIPGLVRGSNKGEGVGNKFLAAIQQVDALCHVV
                     RCFDDADLLHVDGSVNPVRDVETINLELIFADIDMIERRIEKTAKTAKSGDKAYIPML
                     KFYDDLKEHLLEGKMARSFKFENTDQEAYMSDLFLITLKPILYVANVDEQGMSEDNDY
                     TKLLTEIAAAEGNEVIKVCAKFEEELSALDKDEKTAFLADLGITETGLERLIVKGYKL
                     LDLMSFLTVGKKEVRAWTIRNNTTAPKAAGKIHSDFERGFIRAEVFTYDDIKRLGSYN
                     AVKEKGLVRSEGKNYVFKDGDIVLFRFNV"
     gene            80614..81675
                     /locus_tag="PLF14_02610"
     CDS             80614..81675
                     /locus_tag="PLF14_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019908027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT70222.1"
                     /translation="MKEKIKVGIIGTGNISTFHMAGYKRLSDRCEVVAVCDIDEEKVT
                     AFAKKHDVPYYYTDYNKMLSAHKLDAVSVCTWNSAHKDATIAALNSKANVLCEKPMAM
                     NTAEALEMQKAAKRNGKLLMIGFVRRFGNDAKILKSFIDNGTLGDIYYAKATYLRRSG
                     CPGGWFKDIRYAGGGPLIDLGVHVIDLVRYLSDTPKPVSAYGAVYDNLGPNRAEGPKG
                     YVVEDSSKYVFSVEDMATGLVRFDNGLTMFVEASFNLNIKQDTGVIEIFGTKGGAKID
                     PGIEFYLDINGRFVDVKPHGDSALSFTGLFESEIEHFTDCVINKTPCRATAEDGVMVM
                     KIIDAIYESAKLKKEVRID"
     gene            81728..82405
                     /locus_tag="PLF14_02615"
     CDS             81728..82405
                     /locus_tag="PLF14_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT70223.1"
                     /translation="MNKILVVDDDQNICDLLTLYLEKEGYTVVCANDGLEALEVYRDE
                     NPDLIILDIMLPGMDGKDVCKQIKRKDDVPILMLSAKAETFDKVLCLELGADDYVVKP
                     FEAKELVARVKAIIRRTKGNQSGYSDLKFTDLVISKNTYTVLLKDKQIELPLKEFELL
                     FLMASNPNSVFTRDMLLEKIWGFDFFGDSRTVDVHIKRLREKINLPDVNWQLKTVWGV
                     GYKFEVL"
     gene            82402..>82617
                     /locus_tag="PLF14_02620"
     CDS             82402..>82617
                     /locus_tag="PLF14_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70224.1"
                     /translation="MKQNVLSTTLRRFVLYIIIIVSLFSIIIYYQTWFGILNNQSLTA
                     KNIYPLVKSELEQRKILLYSNLTSSAFN"
BASE COUNT        26971 a        13953 c        18576 g        23117 t
ORIGIN      
        1 caacgatatt gatgtaaaat atataaaaca agggctctcc gatgttgtag taaaaggacg
       61 atgcgaaaaa gtcgatacag gtaaagattt taccgtaatg atagattatg ctcacactcc
      121 ggacagtctg gagaaactgc tcattgcggt gcgggaaacc aggacatcgg gacggataat
      181 atgtctgttc ggttgcggcg gtgacagaga taagtccatg agacccatga tgggaaagat
      241 agcggggaaa aatgccgact tttccataat aacatcggat aattccagaa ccgagaagcc
      301 ggatgatata atatccatga ttgaagaagg tattaaagag accgacggcg aatatatatg
      361 tataaccgac aggacggagg ctatttctta tgcgcttcgt aacgcaaaaa aggacgacct
      421 cataatactt gccggtaaag ggcatgagac ttatatggat attatgggta aaaaaacaca
      481 ttatgacgaa agagaaataa tacaaaaggt actgaaaggg gatatatgag aatagagtta
      541 ggaagaatat atgatttgtt tgcaaccggg ttgaaaaccg acaaaaagac cgttgttacc
      601 ggtgtttcca tagactcgag aaccatacag cccggagaaa tattcttcgc cataaagggc
      661 gagaattttg acggtcataa ttttgtcaat aaagctttgg aaaaaaaagc ggctgcagcc
      721 gtctgttcaa ggaaagataa atatgccgga gataacataa tctatgttga agatacggtt
      781 acggcattac aggatctggc aagattctac aggcggtctt tggatatacc ctttatcgca
      841 gttacgggca gtaacggtaa aaccacaacc aaggattata ccggagcggc tttggccgcc
      901 tctttcaaaa ccgattatac aaagggcaat cttaacaatc agaccggtgt tccgttaacg
      961 gtattttccg taggggaaga ttcaaaggct gccgttatcg agatgggaat gaataatgcg
     1021 ggtgagataa ggcaactgtc ctccgtagta ttgccggata tagcggtaat aaccaatata
     1081 ggtaccagcc atataggtaa tctgggaagc agggagaata tctttaaggc caaaaccgaa
     1141 gtactggagt atatggataa cggttacctt atagtaaatg cggatgatga tatgctgcgg
     1201 agggttaaaa acaatcataa gtataaggtg atacgggtgg gaatacttgc cgacgacctg
     1261 gattacaggg cgaggaatat tgtcaagaac gcagataata catatacatt ttcggtaacg
     1321 gatacaaaaa ttaaattatc cataccgggt attcacaata tcgttaacgc attattggct
     1381 tttgcggtgg cggatattct gaaggctgac agaaaaaagg cggcacaagc cataggagca
     1441 tgtactaatc aggtaatgcg gacacagata agcgagattg acggatacac tgttatcaat
     1501 gatgcgtata atgccaatta tgactccatg aggatggcaa tagacatact gaagaactat
     1561 agaggcagaa aggttgccgt gctgggagat atgctggaac tgggtgattt tgaagaaatg
     1621 atgcatgaaa gaacaggaga atatcttgcg tacaacaaaa tagatgtact gattgcggta
     1681 ggtaaaaatg cggactgcta caaaaaaggt gcaacggatt tcggaatgga agaaaagagt
     1741 attcatgtat ttcccgatat tccgcaggca aaaaagcaca ttatggatat aatatgtaag
     1801 ggcgatacta ttttggttaa aggttcgaga ggatcgtcga tggagaagat attggaagtt
     1861 tttgaaggag attttgtatg ataattgcaa taagcgcatc tttcatatta acggttatat
     1921 tgggaatagt gctgctacct gttttaaagc ggcttaaaat agggcaggta gtcagagatg
     1981 acggacctaa agatcacctt aaaaaagccg gcacgcctac tatgggcgga ataatattta
     2041 tatttcccat aatagggtgt atgttgtttc tcggatggaa caatccggtt gtattagtca
     2101 catgcggtct gatattactg tccgctctgg tgggttttct ggatgatttt ctgaagataa
     2161 agagaaaaaa taaagacggt ctgaaacctt tatacaaaac actgctcttt atactgatat
     2221 ccgcagctta cggcatatat atagtatatt tttccaagat aggttcggag ataatcattc
     2281 cctttacaaa tgccgggaag acttttgttc taccgggatg gatttatgtg atttttatag
     2341 tacttttcta caatgcagtc ataaatgcgg ttaattttac cgacggggtg gacggacttt
     2401 gcggatcggt taccgcagtt gtattcgttt tctttgcgat tgcgtcaaaa attgtgtttg
     2461 acgataccga tatgacatac atgtcgctga ttatagtagg ggctatggtc ggttacctta
     2521 tattcaatat ttatcctgcg aaactgttga tgggagatac cggctctctg gctttggggg
     2581 cagcggtaac ggcaatttcc gtaaatctga gaataccgtg gatagtattg tttataggtt
     2641 gcatatacgt aatagaaatt ttatcttcgg ttattcaggt gatgcattat aaacggacaa
     2701 aaaaaagaat atttcttatg gctcccattc atcaccattt cgagttgaag cattggagtg
     2761 agaataaaat agtaatagtg ttcagtgcgg taacacttgc aggcggtata attgcatttt
     2821 tggcaatata ggagcggtaa tttgaaaaac ggtgaaaaga aaaaagcgga ctttctgata
     2881 ttgctatttc ctctggcact gacggctctg ggccttctta tgattttcag cgcttccgca
     2941 ccgtatgcaa agagtcaatt cggtgacata tactttttca ttaaagacca gatgattgcc
     3001 gcattgatag gtatagtggc aatgatgggt gtttctttta taaactacaa attgtataag
     3061 aaattctcct gggttattat cgcgggcgga ttggtgcttt tgctgctgac ttttgtaccg
     3121 ggaataggcg tggaacacaa cgatgcaaga aggtggataa gcattgcggg gtttgaattc
     3181 caaccttcgg aactgtataa actggctttt ataatattcc tgtcaaaaat tattgtggac
     3241 tttaaggaaa aagagaaacc tttagccgga tattttacaa cactggcaat aactttggca
     3301 tccgccgtca ttattttcct gcaaccgcac ttaagtgccg ctattatcgt cattttaata
     3361 ggtttcatta tgatttatgc cggaggaaca aagctcaaat atctgatact gactgttgct
     3421 ccggttgttg ctcttgcaat tatcggtgcc tttaccgtca actatatgaa agacagaata
     3481 atgacctggc tggatccttt cgcatatcct accgacagcg gttatcaggt tatccagtca
     3541 ctgtatgcca ttgcatcggg cggtctgttc ggagtggggc tgggaaagag tacacagaaa
     3601 ttcctttata ttccggaacc gcagaatgac ttcatttttt ccattctggc ggaagaattg
     3661 ggatatgcag gtgtttttct cgtaatagcc ttattctgta tgctgctctg gagaggtatc
     3721 agaattgcaa tcttttgcga agatttattc ggaacactgc ttgccaccgg aattaccgca
     3781 ttgatatttg tacaggcggc tttgaatata gccgttgtga ccggtctggt acccaatacg
     3841 ggaataccgt taccgttttt cagcgcaggc ggtaccagtc tggtaacgtt tttgatagag
     3901 ataggaattt taattaatat ctccagacaa cccataaaaa aggagcgact tacggcatga
     3961 atatactgat agccggcggc ggatcggcag gacacataaa tcccgcactg tccatagcaa
     4021 agaagataaa aaataatcat cccgggtata agatactgtt tatcggatcg gaaaaagggt
     4081 tggaaaaaga tctggtaccc agagagggat ttgatataga gttcatagaa atagagggtt
     4141 tcaaacgtcc tttcggatta aggtcaataa aaacactctt tgttctaatt aaaggattat
     4201 tccaggccgg taagataata aaaagattta agccggatat tgctataggt accggaggat
     4261 ttgtgtccgg tcccgcaata tacatttctt ccttatacaa agccaagata cttattcatg
     4321 aacagaatgc atatcccggt attacaaaca ggatgctggc aagacgtgcg gatgttgcca
     4381 tggttagttt cgagtcggca cttaaatcct taagtaaagc aaaaaagacc gtacttaccg
     4441 gcaatctgat agatgaatca ttttccgact ttgacaagta tcagcctgcc gatggtatga
     4501 aaaccgttgt gataatagga ggcagccagg gagccatgac cattaacaat gccgttacag
     4561 gtatgattaa tgaatatatg aaggagacgg attttaagtt gattttcgct cccggtaaaa
     4621 ggcactatga agaggttacg ggaaagataa ccaaaacact taagaatgta accgttcacg
     4681 attatatata cgaccgcaat accgtatacg cttccgccga ccttatggta accagaggag
     4741 gtgcgaccac aataagcgaa gtgcttgcca tgggtatgcc ttcgataata ataccttctc
     4801 cttttgtgcc ggataacgca caggagaaaa atgccgagca gatatatgaa gcgaatgcct
     4861 gtgagattat atacgataac agtgaattta attccaaggt tctttacgat aagattatga
     4921 atcttttagg tgataaagag aaacttatcg ccctgtcgga aaacgcctat tcgctgggta
     4981 tacgtgatgc caatcagaga gtttatgaag agttttcgga ggttgtaaat gaaattaacc
     5041 gttctgggaa ataacggacc tttccctgca aaagacggag cctgttcggg atatgtagta
     5101 gagaataata aagattgcag aagcggaaat aagactttag acagtaaagg tcttgtaatt
     5161 ttggatttgg gaagcggttg tcttgcaaat ttccaaaaga aattcgacat tgaagatata
     5221 tcttttgttg taataaccca tcttcatttt gatcatatgt cggatttgtt ggtcttgaga
     5281 tatgctcttc aggtgaaagg gagaaaaatg gatatatatt tgccgaaaga gccggaaaat
     5341 gtagcatcta tattgaaatg tgatgagttt aaccttatat atatcgacga atcatcggta
     5401 atagagagag actcttttat cctaaacttt aagcaaacca cacatcccgt ggaaacatat
     5461 gccgtaaaga ttaataatca ttttgtttat tcatccgatt tggcgcaacc ggaagattta
     5521 aaggattttg cttgcggtgt cgatacttta ctgattgatt cggctttgct tgcaaagcag
     5581 aagagcaaaa caaatgccgc tcatctttgt gcaaaagaat gcggcatgct ggcagccgaa
     5641 ctgaatgtaa aaaggcttat actgacacac ttcccgccat attcggattt gagacaatat
     5701 ctcatggagg cttcaactta ttttgcaaat acagtgacgg ctgaaatact tcaggaatat
     5761 taacagatta ttgtgatatt tcctgcggtt tttgaaaaga tttataaaaa gttcatgaaa
     5821 agttcataat gttttgcagt aaaatgcttt taattgatat gttaatgggc tataatgcct
     5881 acataaggat ttcaaatgat taaaaaagca tatatttcca tactactgat aatattattg
     5941 atttgtttga caggttgtat gtcaatcttc cccaaggaag atgagatgct gttattgcct
     6001 gttccctcaa cggctcccgt ggagtataag actataaatc ccacaaaagg agatattgta
     6061 aactccataa ccattctttg taatgttgct aacaggtatg agaacctgca ggatcagttt
     6121 ttttcggtag acggatatct ggaagaattg tttgtggaaa acgaacagta tgtagagaaa
     6181 ggacagttga tagccgtact gtccgaccgc gaagacagag agcaggtgga gaacacttat
     6241 ttaaatgcct tgaacgagtt tataaaggta caggacctgt atgtgaagaa tgccgccacc
     6301 gagtatcagt tcagaagggc ggaaattgct tataatcatg caaaagagaa gtatgacgac
     6361 ctttatactt acatgaccaa taaggagctt cgggcatcac aatcgggctt tatagtaaat
     6421 attcgttcct ttaccaatga tattgcaaaa ggtactccta tcggtaagac tataagattc
     6481 tgttctattg caacgggaca atataagagc atcagcggta ccgcatccgc tgccaatgcc
     6541 tctaaattga agcccggagc gagtgtgata attgtcagta aagaaattga atatgacgga
     6601 tatgtgttaa catccgaggg aacaaatctt tcgttcggat tggatgatat ggatgcggta
     6661 actgcgggag aagtagtgca aatcaaagta ataattgatg aagtttatga tgtactcaaa
     6721 ctcccgaaat atgctgttac tattagagat cagaaaacag ctgcggtaag ggtattaaaa
     6781 gataataccc ccacgaatat ggaaataaac ataggattgg cttcatcttc ggaagtggaa
     6841 atacttcccg ggcaaggtat tgatgaggat actatgatta taacggggac caattaatat
     6901 gctgagactc atattcagaa aaattttgaa taataaatgg atggttatct gtcttatcat
     6961 agggtgtgca tttgccatcg gggtagctgc ggcaattccc atgtatgctt cgggtatgta
     7021 caacacaatg ttggtaaacg aatttaaaaa agagcaggtg caatttgata cttttccgtt
     7081 taaagcatca tacaatgtga catttttcgg actgggaagc gatacctcca agcgagtata
     7141 cgaaaatctt gataacactt tctataacaa atacatggct aatatgccgg ttgaagttat
     7201 gaaggactgc aacagtgttg ccaccaaaac ttattccgtc ctggatcaga acaattacag
     7261 gataaaacag gataatcttt tgaaggaaga aaagccgtca tatagaaact ttacgataaa
     7321 gacggatttt tgtcttactt ccgttgtaga tgcagagact gtttttgatg ttgtgggcgg
     7381 acgcattatg cagacgggta ccgaagaagg agtgattgag gcggtaatat ccgaatatgc
     7441 cgccgcttta tatcggatgt cggtaggcga ttactatttt gtctacaaca agcccgacga
     7501 tgatgatttt atagcaaaaa ttaaaattgt aggcatagta aaggtaaatg aatcttcggc
     7561 ttcctttttc tctgttgcgg aaagagcttt tattatagat tttgacatta tgcaggaaat
     7621 gttaaaagaa tcacggatta ctttaaatca ggcaaactgg tttgcagctt ttgactatac
     7681 caccgtccgc ttaagatatg ccaatgatat ctcacaggct acgcaggctt tatccacatc
     7741 cagcagtgtt acgggcgtaa ttataaatac atcgaacaac tatatttcca ttataagcaa
     7801 ctataatcgt gacatgcaga ctatgcagaa gatgtttctg cttctgataa taccggtcat
     7861 tttgatgata gggatgtatg ttgtcatgat ttctcggttg atgatggaca gggaaaaatc
     7921 ggaaatagcc actttggcga gcagaggtgc aagcagaaat cagatttttc ttatctatct
     7981 gggacagggt attgtcatag cggtagtttc ctttgtttta ggtatattgc ttgcgcgatt
     8041 gctgtgtcat cttctcggag cggcaaacgg attcatggaa tttgtcaaca gaacggcaat
     8101 tgaagtcagg tttgaaagca cttctctgtt gtatgccgta gtatgtgctc tgatatatct
     8161 cataatgatt ctggtacctg catacaaagc atcaaagtat acaattgttc agctcaagca
     8221 gagcatagca gaccaatcca ggagatcttt ctggcagatt atgttcttgg atgtcattct
     8281 gattggtgtc tcgctatatt tcctttatga ttatgaacaa atgagcaaat atatccagga
     8341 tgtgcaatcg gcacaggtgg attttacatt gttcctggcg gtaaccatgt ttatattggg
     8401 attggggtta ttgtttatca ggttgttccc ttatctgacg gaacttgtgt ttatcatagg
     8461 aaagaggcat tggaaaccgg catcatacaa tgcattaatg catgtttccc gcggcggtaa
     8521 ctacaagcat tttataatga ttttcctcat tcttacgata ggcgtgggta ttttcaatgc
     8581 caattccggt aggactctta atgagaatct ggaagataat atccgttatg aaataggtgc
     8641 ggacgcatct tttgtaccta ccctgattgt actcaatgat aagggaactt tcgattacaa
     8701 acccattaaa ggtgcagctc ttatgtcttt tgcgcggtta tactgtaatt ccgatttcgt
     8761 tgaaaattac actacatatt attattacaa gaccgaaacc aggccttcaa gagaacctat
     8821 agtatttatg gggattgagc cgtacgaatt cggcaatgtt gtaaaattac ggaaagattt
     8881 gaatgaaggt attcccatta atcacatatt gaatactctg tcctcttacc ctcagggcat
     8941 gataatatcc agatcgctcg caaacgaata cggggtgaaa gagggacagt ttatgaatta
     9001 tgtgtatacg tctgttatag aaggtgtgac tgtcgaaaat ttagtagaga atgcccttgt
     9061 ggtggcggta gtggattact ggccggcaat gacagacgaa cgctttattg tcatgacacg
     9121 tgacaactat ttcgtaatga aggattttga tacaagaggc aggatattga tagataaaaa
     9181 agacggtgtt ttggacagtc aggttctggc ggatataaaa ggaaaaatgg gaccggaaga
     9241 aaccatagac agtataaatt tcaccgacta taatgtaacg ttggctaaga agaattccat
     9301 tctgaacgga atgaacggaa tattgacact ggatttctta atatccatga tagtgtgctg
     9361 tatcggtttt ataattttct ggattatatc ggtcagacag agagtattgc aattcggtat
     9421 tttccgtgca atgggaatga acaaagggga attatacaga atgatattct atgaacagct
     9481 gatgatttct ttcgtatcgg ttattgcggg tattgtgata ggcggtgttg ccagtcagct
     9541 gtttgttcct ttatttgaga gaatgtcgac attaagcaat caataccttc cttttgccac
     9601 ttacagagac ggaattgatt acataaggat ttatgttata acgggtgtat cgttaattat
     9661 cggattatcg gtgcttataa ggttcatatc caaattaaag atagatcagg cactgaagtt
     9721 aggagaagac tgatggctgt attattgaga accgtggata tatgcagaga ttttaagcag
     9781 ggaagccaga cagtacatgc tcttgtggat gtaaatgtat ccatacaacc cggtacgctt
     9841 acaatgcttg caggcaggtc ggggtcgggt aaaacaacgc ttatgaatat agccggtgca
     9901 ttggacaggc ctacttcggg acagatattc tttcatgaca gggagatttc cgatttaagc
     9961 gagaataagc gggatgaaat aagaagaaag agcatgggat tcgttttcca gtcggtagct
    10021 ttaatatccg tactcaatgc atatgagaat gtcgagttcg gtcttcgtat ggcgggggtg
    10081 catcccaaga aacgggacag gctggttaag gaagcattga catttgtcgg tctggaaaga
    10141 agaatgaaac ataaaccgca ccagctttcg ggaggagagc agcaaagagt tgccattgca
    10201 cgctcaatag ttgcaaggcc ggaattaata tttgccgatg aaccgaccgc ggagttggat
    10261 acaaagacag gtttgagaat agttcgactg tttcggcatc ttgtggatga agaaaaactg
    10321 acaataataa tgacaacgca tgatccgagt atgattgagc ttgccgataa tgttataagg
    10381 ctggaagacg ggagggtagt gaaatgatta aaaagataaa atcattcttt aataccgtct
    10441 ttaaaaaaca caaaaccgat tctgacgaga tggagaccaa gaatttctac ggtcaggatg
    10501 agtcggaaat accgggttac acacccgatc atatcatttt ttgcgacggg ttggtaaaga
    10561 tttataaatc caaggatacc gaagtggttg ctctgcaggg gctggaactg gagataaaaa
    10621 aaggcgagat gatggcaata ataggtaaca gcggaagcgg taaatccact ctgatgaact
    10681 taataggagg tctggacgtg ccttccgcgg gaacgctgat agtggacggt gaaaatctgt
    10741 taagaatgaa aaagtcggaa ctgctgagat ataagaagga gaaggtaggt ttcgtgtggc
    10801 agaacaacgc aagaaacctt ataccttatt tgccggccat tcataatgtg gagataccca
    10861 tgatacttgc aggcaggaag gatgccaaaa agcgtgccgc tgaactttta gatttaatgg
    10921 gacttaaaga cagaatgaaa aaccgtttgt cacagctttc cggaggagaa cagcaaagag
    10981 tagccatagc catagcaatg gcaaacgacc ctaaaatatt acttgctgat gaacctaccg
    11041 gagcggtgga taccgctact gcggcattga tatacaatat tttccgaaaa ctgaatacgg
    11101 aatacggagt taccatcatt atagtcacac atgacagagc aatatcaaag aatgtggaca
    11161 gggtagttac aattgaagac ggaagaacgg gaagcgagta tatcaagaaa aaatcctata
    11221 aagaacagat aatggaatta gacagaatga ccggagtgga agagtcggaa actcatgagg
    11281 aaatgattgt agtagacagg cagggacgct taaaaatacc cgaagaatat cttgaacttg
    11341 ccaagattcc caacaacagt aaagtgcgaa ttaagatgga agaagaaaag attgttatat
    11401 atacggagga gaaagatgaa gaataaatca tttattatcc tgtttattat tgccgccgta
    11461 atgctttcat ttcttacagg ttgcacatat ttctatccgt tggaaggcag atatcatgcc
    11521 gtacagccgg agctgattga gcaaaaaaag gtggaattgg atagttttga agtaagaaaa
    11581 accgatgtat ttgagggatt tgacttaggt gaagaattcg gaagacgatt agtatatgtg
    11641 aacaatccga aaaaaactac aatagtgcaa agcgaaatag cccgggctaa gatacaaaag
    11701 ctgtatgttg aacccaacga ctatgtcatg gaaggcgata ttttagtaga gtatgtatat
    11761 gaagtaaaca aagatgcgga gtttcaagtc aattggaata aaagaagagc ggaaatacga
    11821 tatgagaatg catatatcca gtatcagcag ggaaagatta acgagcagac tctgcaaaac
    11881 cttcaaaaag cctatgcggt tgcaaaaaac gctctggact cctttaatgc cataccggac
    11941 aaatatacat tgagagcacc ggttgacggt tacatagcca aaatagaggg agatacggtt
    12001 tccacggatg aagtgcagac actggttttt catatatgca aaattgaaga cggtatcctg
    12061 ttgacatatg cacgagaaca gaatgaatat gccgcatacc cctttacgct gtgtttcgaa
    12121 ggcaccggaa tggaattata tgacgagcaa tcggatagga catattccgg ccgtgtcagc
    12181 atgagcaata tgacgtttaa aaaagagtat gtaaatgatt tgagttattt cagcggcgga
    12241 gcctacttat atctggcatt ggagcttaac gataacaaac ttcccgaaga tgtggtgttc
    12301 tatcaggagt ttaccggtaa attgataaga agagaagcgt acgatgtatt gattgtttcc
    12361 agaagtgcag tagatatgga aaaaacggac gacccggagg aaccgtttgt atatcaggtc
    12421 tataagatat taccggatga gactatagag cgtgtccgaa ttaagatcgg cgatattttt
    12481 gatgcttatt acgaagtatt agaagggttg gaagaagggg ataaggtagt attaagatga
    12541 tagaaaagag aacaaacctt ttaatttcaa ttattataat aatagttttc acagctattc
    12601 ttacatcgtg tgctacccaa gcggttactt atgaaatacc tgtttttgat ataccgaaag
    12661 aggtatttac cactttaaag gtggaaaaac aggatataca tgattacgaa agaatcaatt
    12721 tggatgctca gagtttagga ggaagcagag gcatagtatt ttcctattcc gatttcgagt
    12781 cggcaacact tctggtatcc gaacagaata ctgttgtgaa agaaggggat gttcttgctg
    12841 tcaaaagcga taaaacttta gagtatgatt tgcgtcttaa actggaagat ttggaactga
    12901 aacaattgtt atatgaagaa ctttacagaa agtatctcaa taccggtgag ggatactatg
    12961 atatgcagtt ggctttatcg gatatggagc ttgcacagtt taattatgac aaagcattgg
    13021 aaaaactgga gacgcttcag tttaaggcac cttacagcgg aacggttaac aaagttgtaa
    13081 cgacatacaa aaatgagaaa gatgaattca ataatcaata caaggtgtat tcggtaacat
    13141 attatattac tcccgataat tacagtgtca gtttacagat aacagctgcc ggatatcctt
    13201 attttgaaga actcggatat aaatcaggtg atactcttca gatatatgat aaagacggga
    13261 aaatccataa tatgataatt aaatcaatat tcaccagaac aaccgaactt gccggttcaa
    13321 aaataaaaac tgcttttgta taccttgagt ttataaaaga agaaggtgtt gattacggcg
    13381 aactgataaa atatgctctg ttctatgata tgacactggc gaaatatgat gatgtcattg
    13441 tagtaccggt atcatatgtc aggaagacat ccgagggtaa agactttacc tatgttctgg
    13501 aaaacggcag aaaggtaccc cgcactctta tagtcggcaa taaagtatta aacggcaccg
    13561 cataccttgt attggacgga ttgtatgaag acgatcttat aataaccggt tcgtcatttg
    13621 agtgattttg gaagttttta catattatta tactcaatag attacagtaa ataattatat
    13681 cgggtagaaa taaatgctga gacttatatt cagaaaaata tggaataata aatggttggt
    13741 catctgcctc ataataggct gtgcattcgc tgttgcggtc gttgccgcaa ttcccatgta
    13801 tactaccggt ttatataatt tgcagttgtc cgaggaattt aaaaaactgc aggaagagtt
    13861 tgattctttt ccgtttaagg catatttaaa tgcaagtttt ctgggtatga aaaaaagtga
    13921 ttcgataaaa acatttaacg atcttgacag caaattttat gaccgatatg ttgcaaacat
    13981 acctgcaaaa aatatattcg attataaaag tatatccacg gtttcctatc ccgtttttga
    14041 taaacgaaat tatgaaatca aacaggatat gattgccaga ggagaaaaac tttatacaag
    14101 gaatcttaca ataaaatcga gttttacaat aacttctttt gcaaaagtac cggatatgat
    14161 taaacttgtc ggagggcgca tgtacagtac ggatacggaa gaaggagtag tggaggcggt
    14221 tattacaaaa aactccacta taatgaatcg catttcggta ggtgatactc tatatgtcta
    14281 caataaaatg acggacattg atttgcttgt aaaaatagta attgtcggag tagtggaaaa
    14341 taccgatgaa tcggctgcat tttacacatt gtccgacaaa acattcctga tagattacga
    14401 taagatgctg gaaatgatag gtaatgagga gataaccttg caatcggcca attggttttg
    14461 cgcatttgat tatacaaccg ttaaacttcg gcacagtgac aggataatag ccgctaccaa
    14521 ggttctggat tcatatgatg ccgactcaaa ggttatgctg tcctcttcaa acagttatat
    14581 gacgcttttg gaaaaataca gtgaagatgt taaagcaatg caaagactgt acatcttact
    14641 gctgttgcct gtaatattga tggtcggtat atatgttgtg atgatatcca ggttaatgat
    14701 ggaaagggag aaggcggaaa tttccgtttt agccagcaga ggtgcaagca gaagtcagat
    14761 tttcttgata tacttggcac aaggtatagt catctccgtt atttcatatg ttctgggaat
    14821 tttaattgcg cgatttttat gtcgtctgtt gggggcggca aacggattta tggaatttgt
    14881 aaacaggacg gcaattgatg ctaccttaac ttctgccgcg ttattgtatg ccgcaatatg
    14941 tgctacaaca tatcttatac tgatactggt accggcatac agagcgtcaa aattcaccat
    15001 tgtgcaacat aagcagaata ttgccgaaca atcaaaaaag tccttctggc agattatatt
    15061 tttagatgtc attcttatcg gtatcgccat atatttcctt ttcgattatg aatcggtgaa
    15121 ggagtatata cagtttgcac agattacctc ggtggatttt actttattcg tagcggtgac
    15181 attttttgtg ctgggtacgg gtttgttgtt tataaggctg tttccgtatt tggtggaatt
    15241 gattttcagg ataggaaagg agcgatggaa acccgcttcc tatgccgcat ttactcatgt
    15301 ggcaagggga gggaattaca agcagtttat tatgattttc ctcatactgg caataggtat
    15361 cggtatattc aatgcaaatg cgggaagaac gcttaatgag aatcttaaag acaatctgaa
    15421 gcattccata ggtgccgata tatctttcgt gccctcggtt cttgaatatg atttcatttt
    15481 gaacgggtat tattttgcgg acaacagagg accgggtcta agtaaacagg cccataaata
    15541 ctgtaattcc gatttgattg agaactatac cgtctattac tatcacaata ccactttaag
    15601 accttcgaat caatcggtaa cttttatggg tattgaaccg ggtgatttcg gcagggttgt
    15661 aaagataaga aaagatttga atgaaggcgt acccttaaat cacatattga atattttgtc
    15721 aacatatcct aaggggataa tcatatcgcg tagtatttca cagctttata acgttaaagc
    15781 aggacaatat ttcgatttta cctacacaaa cagagcgttt cgggaagtgc aggagattcc
    15841 cgtgaaagac gtgcttgttg ttgcggttgt ggattattgg cctacaatga cggtagataa
    15901 ttttattatt atgacgcaga acaaatactc ttcgatgagg cattttaggc agaacgggaa
    15961 ggtgctggtg gataaaaaag aaggcgtcag cgataatgag gttctggaag atatgaaaac
    16021 aaggatgtcc tttgacgaat caataggcac aaattctttt accgattatg aagcaacaca
    16081 ggcaaagaga ggatcggttc tgaacggaat aaacggaata cttacattgg attttcttat
    16141 atccatgctt atatgctgta taggttttat tatattctgg attatatcgg tgcgacagag
    16201 ggtattacag ttcggtattc tccgtgcaat gggtatgaag aaacatgaac tgtacaggat
    16261 gatattctac gagcagctca tgatttcctt tgtatcggtt ttagcgggca tattaatagg
    16321 cggcatagca agtaagttat ttgttccctt gtttgaaaga atgtccactt tcagcagtaa
    16381 ttttcttcct ttttatgcct accgggaggt tgtggattat aacagggttt acattatcac
    16441 aggtgcggca ctgacaatcg gtttatcggt aattttgaga tttacatcca aacttaaagc
    16501 ggatcaggca cttaaactgg gtgaggactg ataatattac cgctgaactg acggtataaa
    16561 gtttaattta tctttccgaa caatcaatcc gttatttcat tcaatttaac attgtccgtc
    16621 ccgatatccg acttgtatat aaagaaatct atgggcttgt gagcggagga tgccagtaaa
    16681 acggagattc cggtatcatc aataaagcat cctgtatcca gtttcaaagc atcaataatg
    16741 caagagttca atatgtgata ttgcttgtca agtatataca aagtctgact gtcaaagtat
    16801 ctgggatagc ctctgctgtc agaggtcttt gcaaaagcaa tgatgtaatt atagtcggga
    16861 tagaaatatg caagagaacc ccaagcatct tcaatcttaa aatcggcaac tttgttcatc
    16921 tgttcagacg taagattcaa ttcatagaaa ccgtcattat gtttgtagca gtatgcgttt
    16981 tcccaatcga aatgagagtt tctgtagtcc gcccacttat ccgtcaataa acgcatattc
    17041 ttatccgtaa gagagagcat atacagttca tactcggcgg ggttctcatc gtacagttta
    17101 tagttttcat tgtagggacc tgttctgaca taaaagaata cattgtcacc gacaagcgta
    17161 aacatgcaag cttcgggtat tacatcatga ctgataaccg actcatattc cgaataatca
    17221 tccaatttaa cacgatagaa attgttgtcg gtgttgtact tcgagcccat ttcctgtcct
    17281 gcgttaatca ttacaaaata gtaataaccg gaatgaaaaa accctaagca tctttttcct
    17341 tgtgtcagcg acacggtcgc aaaagttttt acaagttgac gttccgtacc gtccatattc
    17401 attttgataa gattccaatc taaaccgttc ttttttgatc tctcaacata atatatgtta
    17461 ttttcataat aacctaactg aatagttttt tcgtttgtgg gaactatttg atcaattttc
    17521 ccgaggtatg catcacaatc atcatccgta tgggtacatt cggcttttgt acataattta
    17581 acaaactttg aatttccgta ttgcgaatag agaataaaac cgtcaacata atgatattta
    17641 ccgtaggggg tattcagata ggaatgccat acccgtgctc cctggttcat ggatgtataa
    17701 tactcatccg gtaaggataa atccggttgc agtataacta aatccgattc aactgtattt
    17761 gtcggtgtaa aactgatttg tgtttcgttt cggcaggatg acagcacaaa acataatatc
    17821 agcaaaacag ctaccgttaa gtatagtttt ttattcatga taaaatctcc ttgtattgaa
    17881 atatgtacaa tccgagcgga ttaaccaatt cacttattat tatacttatt ataaagctcg
    17941 tttgaagtgc tttgggtaaa agttcattaa aagttcatat ttcgatataa aggaaggcat
    18001 ataatcagca atcaatgtgt aatctcatac aatttaagac ttttcattcc gaaatccgat
    18061 ttatatatga aataatcggg cggtttatgt gaatcggtag acagtacaat acagttaccg
    18121 gtatcatcaa ttaagaatcc cgcttccgta cttgaagtcg gattattgta aatattcaca
    18181 atgttatact cccaatcaag tatatacaaa gtctggtcgt cataatatct gtcatgacct
    18241 ctgttattct tggtcttttt aaaagcaata acataaatgt tgtcgggata gaagtgagca
    18301 accgttcccc aaccgccttt aattgcggaa tcggcaactt tggtcttctc ctttgtggca
    18361 aggttcaata catagaaacc gtcattatgt ttatagcagt atccgttctc cgaatcgaaa
    18421 tagcagttgg taaaatccga ccagttgtcc gtcaataaac ataattcatt gtctgtaaga
    18481 gatagcttat acagttcaaa atctacagca aagttttccg ggtttttggt accttcagtg
    18541 taggttcccg tccggacata gaagaataca ttattgtcga ctaaagtaaa tcggtaagcc
    18601 atcggaatta tatcatggca gaaaaccgat tcatacggag aatcgtcgtc caatctgatg
    18661 cgatagaggt ttttatcggt gttgaaccgg gaccccatgt attgatcgtc ggtcaataca
    18721 aaatagtaat aacccgagtg aaagaaaccg tgcatttcct tgatggaaag ttgtatatcg
    18781 gtaataattg cttttacggt ctgacgttcg gttccgtcca tattcatttt gtaaagtgtc
    18841 attaacatat tgcccgctct ttgcacaaaa tatatatttc catgataata acctatctga
    18901 gtgttttttc cgacatatgc atcacaatca tcgtacttat gcggacaatt ttctttttga
    18961 cataatgtca taaatttcgt atttccgtat tgggaatagt gaatgaaacc ttctacatta
    19021 tgatatttgc cgtagggagt attgagatag caatgccata ttttagctcc ctgattcatg
    19081 gatgtgtaat actcatccgg aagagacaag tccggttgca gtataaccaa gccggtatct
    19141 tcggtttccg tcggtgccgg aactatttgg gcttctttac ggcaggagca taatataaag
    19201 gacagtatca gcaaaacagc taccgctaag tatagttttt tgttcataac agtttctccc
    19261 gtatttgata actttcgtac tatccgaacg gattagtaaa ttcatttact attataccct
    19321 atattaagcc ttttttaaag tgccttgagt taattcagtc aactgaagtg atgaatcggt
    19381 gcattatttc ttcttttgtt aattatttat cttctaattg ttgtataatg tatgttgctg
    19441 attagcggag gagaaatgga caaaaaaggc aacaaacttg gtgaatggat taataatgag
    19501 cttccggaaa tagcatcaca actggaagca ctcaatgacg actattattt acgtgaaaac
    19561 acaatatctc tggcggggaa agagcgggta aagaatatac tgtttctttt acgctcggct
    19621 ctgtttcccg gggtgtatga aaaatgctcc ataagcaaag ccaatgtgga tatgcttata
    19681 ggcaacaact taagggaagc cgcaattgag ttaagagaga ttttagagag tgtattcagt
    19741 tacaagtgtg acaaggactg cgacaattgc ccgattaatc ttgatgaaat tattattacc
    19801 ttcctggata aaatgccaaa aatacgaaga aagttacaga ccgatattca tgccgcatat
    19861 gtgggtgacc ccgcagctca gagttttgag gagatattat taagctatcc gtcactggag
    19921 atgatgagca ttcacagaat agctcacgag ttatataaac tcaaagtgcc tttaattccc
    19981 agaatgatga acgagtacgg tcacagagtt accggcatag atattcaccc gggagccagt
    20041 ataggagagc atttcttcat ggaccacggt accggtatag taataggaga gactacggaa
    20101 ataggggata atgtaaagat atatcagggt gttactttag gtgcaaaaag ctttaagttg
    20161 gacgcaaacg gcaatcccgt taaaggtata aagcgacacc ctaaaataga agacaatgtg
    20221 gttatatact cacatgcaac aatattgggc ggggatacgg taatagggca tgattcggta
    20281 attggtgcgt cggtatggct tatggagtcg gttccgccgt attccaaaat ttactataac
    20341 ttagggagtg acaaatagat tatggctgaa ttaagatggc atcctctgat aaaagactgg
    20401 gtaatggtgg catcgcacag gcagaacaga ccgcagatgc ccaaaaactg gtgtcctttc
    20461 tgtaaaggca gtgaaaaggt accgaaagac tatattgtca tgaaatacga caatgatttt
    20521 cccgcactgt ctcaaaatcc tccgattccc gataatgtgg caacggattt gtataaaacc
    20581 gcaccgttgt acggtaaatg tgaagttata ctgtattctt ccgatcacca tgcgacatta
    20641 cccgaattgg acgatttgca tctgaatgaa ctggtaaaac tttggaagca gcggtttatc
    20701 gagatatctt ccgataagtt gataaagtat gttttcatat tcgaaaacag gggagaggct
    20761 gtaggagtta ccatgccgca tcctcacggg cagatttacg gttatccttt tatacccaag
    20821 aagttggagc tggaattgca atcttgcaaa gaacatgccg ataataccgg cagatgtctc
    20881 atatgcgaca tgaacggaga agaaataagt tttgctcaac gggttatatt ccagaacgac
    20941 agtttcatag tttatcttcc gttttttacc gaatatccgt acggtatata tatttcttcc
    21001 aaaagacata tacaatatat tactcaattt accgaaaagg aatcatcgga ctttgctaag
    21061 actttaaaga ttgctgcagg tacgttggac agtcttttcg gatttacatt cccgtatatg
    21121 atgtgtatgc atcaggcacc ggtaaatacg ggtgagcact atgactatta tcattttcat
    21181 gtggagtttt tccctccgat gagagcgaga gataaaataa agtacaatgc atccagcgag
    21241 accggagcat gggcacactg caatcccact tctgtggagg agaaagcgga agagttgaga
    21301 gcggcatata gaagatttgt aacgggaaaa ggaaatacaa aaggtgaata gtttatgata
    21361 aaagaagagt tggcaaatat tttgaataat gtatacggca gttccgagga gagtatcaga
    21421 tactttcagg cgcccggaag agtcaattta ataggggaac atatcgatta caacggagga
    21481 tatgtttttc ccgcggcaat agataaagta tccactgttg ctgtaagaaa aaggaaagat
    21541 gacataataa aactgtatgc taccgatttg ggttatgtag tggaagcgga catgaatagt
    21601 cttgaacagt ataaagactt gaaatggggc aattatcagc tcggagtcat tgatcagatt
    21661 ttaaagaaag gcattaaagt aaaaggtgcg gagttcatat ttgacgacaa ggtgccttta
    21721 ggaagcggtt tatcctcatc tgccgcgatt gaggttgtga ctgcatatgc aatgttgcaa
    21781 ctttacggcc ataaaacgga caagatggac atagccctgt tgtgtcagaa ggcggaacat
    21841 gagtatgtcg gcgtgaaatg cggtataatg gaccagtttg cttcggcaat ggggttaaaa
    21901 gaccatgcca tgctgctgga ttgtaattca ttggaatatg agtatatacc tataaaactt
    21961 gacgactatt cgatagttat atccaacacc aataaaaagc ggtcgttggg ttcgtcaatg
    22021 tacaacaaaa gaagagcgga gtgtgaacag gcgttggccg atatgcagaa gaagtttccc
    22081 gacaagttga atttatgtga ttttacctat aaggagtttt tatcctgtgc agacgatatt
    22141 accgatgccg attgcagaaa gagagcggag catgcggtat gtgaaaattt acgggttttg
    22201 gacagtgtca aagcattaaa gaaaggcgac atatattctt tctgtaaata cctcaatctg
    22261 tcacatattt ctttacgtga tctttatgag gtatcgtgta aggaactgga tgtgcttgtg
    22321 gaaagtgcat ggatgcagaa agatgtatta ggttccagaa tgacgggagc gggtttcggc
    22381 ggctgtacgg tttccattgt gaagaagcaa tcggtaaagg attttattga agatataggc
    22441 agagtttatc aggaagttat cggatacaac gcatctttct atatctgcga aatttccgac
    22501 ggggttaagg agatatagat gagtatttta gtaaccggag gagcaggtta tataggctcg
    22561 catatggtac tgcatttgtt ggaaaaaggc tatgaggtgg tagttgttga tgatttatct
    22621 accggtaata aagaagcggt gcttgcaaat aagttttatc agacggatat taaagacgaa
    22681 aaagccatgg caaagctgtt taaagagaat gagatagaag gaataataca ttttgcagca
    22741 tattcactgg taggcgaaag catggcacat cctctgaaat attatgagaa caatgtggcc
    22801 gggacattgc agatactaaa gtatatggat atgtacggta taaataatat agtgttttcc
    22861 tccagtgccg cggtatacgg ggaaagccct tctcccataa aagaaactgc cgacaccttt
    22921 cccacaaacg catacggtga aaccaaactg gcggtggaaa gaatgctcaa atgggcttca
    22981 aaggcttacg gtataaatta tgcggcattg agatacttta atgcagcggg aagccacgta
    23041 agcggcagga taggggagaa aagggctgtg gaaacacacc ttatacccat tgctttagat
    23101 gtagcattgg gaaaaataga taaacttctg gtattcggag atgattatga tacctttgac
    23161 ggaacctgca taagagacta tatccatgtc agcgatctgg cgacggcaca tcttctggca
    23221 ttggaaaaga tgataaatga ttctattaac ttggaaagga agagcgggaa ttccggtatt
    23281 agcatgatat ataacctggg aagcgaaaag ggtttttcgg taaatcagat aattgatgcg
    23341 gtaaagcatg ttaccggtgc ggatataaag acggaaataa caaaaagacg acaaggagac
    23401 cctgctgtac ttattgcgtc cagcgaaaag ataaagaagg aattgggatg ggtacccgag
    23461 tttacggaca tagaggatat tatagccacg gcttgggagt ttcgaaagaa aatgaggtga
    23521 aaactaaaaa tgaataagaa acttaaatca aaagagaccg actatttatt tgatgcaata
    23581 ctttctttga aaaacaaaga cgaatgctac atctttttcg aggatttatg caccgttgcg
    23641 gagattaaag caatggctca gcggctttgt gttgcaaaga tgttggataa caagcataca
    23701 tatgccgata tttcagtaaa gaccggggcc tcggctgcta caataagcag agttgccaaa
    23761 tgcttgaact acggagcgga agggtataca accgttttga aaagattagg agaaattaat
    23821 gggtagaata ctggataaga ttttcggtac atacagcgac agagagatta agaaaatact
    23881 gccgcttgtg gaagatatag aggctctggc cgatgaaatg aagggtaaga ccgatgcaca
    23941 acttcgggca tatactaatc tttttaagca acgtctggaa gacggagaga cattggacga
    24001 tattcttgtg gaagcatttg cggtatgcag agaagcttcg accagagtac tgcatatgac
    24061 cccgtataaa gtacagttga taggaggaat agtactgcat cagggcagaa ttgcggaaat
    24121 gaaaaccgga gaaggaaaga cacttgtcgc tacgctgccg gtttatctga atgctctttc
    24181 gggcaaaggt gttcatgtag ttacggtgaa cgactacctg gctaaaagag acagcgaatg
    24241 gatgggaaaa ctgtataact tcttgggact gtcagtcggc ttgattgtct acggccttac
    24301 caaggaacaa agagaaaacg catataattc cgatataaca tactgcacga acaacgagtt
    24361 gggattcgac tacttaagag acaacatggt cacaagaaaa gaagagctgg tgcaaaggga
    24421 tcttcacttt gccattgtcg acgaagtgga cagtatttta attgacgaag caagaacacc
    24481 tcttatcata tccggagcgg gagaacagtc ttccaatttg tacaaacgtg cggataattt
    24541 tgccaagaca cttaaaaaga aagtggttat tgaagaagac agcggcggca aactgcaaag
    24601 aataatggag ttgaccggtc aggaagaaga ggatgagtat gctcccgaca aacccgacta
    24661 tattgtggat gaaaaaaacc ggacggcatc gcttacaccc aagggagtgg agaaagccga
    24721 acgatatttc aatgtggaaa atctgacatc gtatgagaat accggtcttt tgcatcatat
    24781 aaataatgca ataaaagcac agagcataat gacccgcgat gtggattatg tggtaaagga
    24841 taaccagatt ataataatag acgagtttac cggcagaatg atgttcggaa gaagatattc
    24901 cgagggactt catcaggcaa tagaagcaaa ggaaaatgtc aaggtggaaa gggaaaacaa
    24961 gactcttgcc acaataacct tccagaactt tttccgtatg tacggtaagc tttccggtat
    25021 gaccggaacg gcattgaccg aagaggagga attccgggag atatacaaac ttgatgtaat
    25081 cgctattccg accaataaac cggtaataag ggaagattat cccgatgtgg tctataccac
    25141 cagaaaagcc aaattagatg ccattgtggc cgatataata gagtgttctc aaagacagca
    25201 gccggttttg ataggaacca tatccattga aaagtcggaa gaactcagtc ttatgctgag
    25261 aagaagggga ataaagcatg aagtactcaa tgccaagcat catgaaagag aagcggaaat
    25321 tgtggctcaa gccggaaaac ccggagcggt aaccatagct accaacatgg cgggcagagg
    25381 aacggacatt gttctcggag gtaatgcggg atatatggct atgcagaaac ttaaaaaaga
    25441 gatatttgat gaaaatattt tactgcaagc agacagtttt aatgagaccg acgatgaggc
    25501 ggttatagag gcaaggaacc tttaccgtca gtatgaggcc gacttcaaga aacttattga
    25561 agaagacaga aagagagtgc tggaaaaagg cggcctgtat atacttgcta ccgagcggca
    25621 tgaatccaga agaattgaca accagttaag aggtagagcg gggcgtcagg gcgatcccgg
    25681 tatgagcaga ttctatctgt cgctggaaga tgatctgatg cgtctttttg cacaggacag
    25741 attggccaat atgatgaatg tactgaatgt tcccgatgac atacccattg aagccaagat
    25801 aataaccaat accattgaaa cggcacagaa gagaatagaa ggtgtcaact tccagagacg
    25861 taagacggtt attcagtatg acgacgtaat gaatactcag aggaacctta tttacagtca
    25921 gagaaggcag gtacttgacg gtaccgatat gaaaggcagt tatctgaaga tgttagagtc
    25981 ggtacttacc agactgacag atgattactg tcagagcaac aggtcaaacg attggaatat
    26041 gcaggcaatt tccgacagat taaacgaaat gctgcccaaa caatataacc cgttgttgaa
    26101 aggcaatacc gccgatgccg actacaatga aaaatccttc cgtcagatgc ttatagactc
    26161 atacagtaag gattatgacg acaaagaggc ggaactgagt ccctccctta tgagggagct
    26221 ggaaagaatt atactgcttc ggacggtgga tatacactgg atggatcata tcgacgcaat
    26281 ggatcagttg cgttcgggga taggattgag agctttcggg cagaaagacc cggttatgga
    26341 gtataagttt gaaggatata tgatgttcga ggaaatgacc aacagcatac aggaggaagc
    26401 actgaaaaaa atattttccg ttcataaaca gaccgaagta aagaatgaag agagcaagat
    26461 aagaataaat gcgcagacgg ctccggatga tgtacggaaa cctgccgttt cctccaatac
    26521 aaaagtcggt cgtaacgacc cctgtccctg cggaagcgga aagaaatata agaagtgttg
    26581 cggagcgtaa tatatgaact atgaggaagc aataaaatac atacactcgg taggcaaatt
    26641 cggttccaaa ctggggctta tcaatataac aaaactgctg gatttactgg acaaccctca
    26701 aaatgatttg aaattcattc atgtgggcgg aaccaacgga aaaggctcca catgcaacta
    26761 tattgctcat atgttacatg cggccggata cagaacaggt ttgttcatct ccccgtatat
    26821 cgagaagttc ggcgaaagaa tgcaggtgga ttttaaaaat atcgataaca gaagcctggt
    26881 agctcatgta cagaaggtta aggactgtat tgatatgatg ctggagcagg ggtacaatca
    26941 tcctactgag tttgagataa ataccgcggt tgcaatgctt tattttaaac agaaacgctg
    27001 taatacggta gttttggaag tagggttggg aggtcggctg gatgccacca atgtcatcaa
    27061 atcaccgtta gttaccgtca tatgcaccat tgagaaagac catatgggtg tgttgggcga
    27121 tacattgcag aaaatagcat ttgaaaaagc aggcataatc aaacccgcat ccccggttgt
    27181 ggtatacgga gacaatgagc cgtgcacata tgatgtgata gaggcacagg cggaaagaaa
    27241 gggagcacct tattttgttg tgaattttga aaatatatca aatatcagat attccgattt
    27301 cggctataca ttcgattttg agggttatac cgatttgcga ataaatatgt tcggtgatta
    27361 ccaggtgaaa aatgcctgca ccgctattaa agccgttgag gcttcgaagt tgaaggtctc
    27421 ggaggaggca ataagaaaag gactttttaa tgcaaaatgg cccggtagac tggaagtatt
    27481 atcgaaaaac cccttgatta tctgtgacgg ttcacataat cccgaagccg taaggagttt
    27541 gaaaaatact cttatcaaat actttcccga taagaaaaag atattgataa tgggcgtgat
    27601 gaagaacaaa gaatatgaaa aaatgattga agatatattt cccttggcgg atatgggcat
    27661 tgctataaga acgcaatgta acagggcttt agaattggat atattgcaac aaaccatgca
    27721 aaaatactgt aaaaatactt attcttttga taagatagaa gacggactgg attatgcggt
    27781 agccaatgcc gacaataata ccgttatttg cacattcgga tctttgtatt atatagcgga
    27841 tgtaaagaac tacgtcagga gaacggggct gtagatggat attaatgaac tgcaaaagta
    27901 tctggaaaag attgatttca acgcattgag taaagacaat gtgaaattaa gcgaggatga
    27961 tgcttatgta ggcgagttat acaatagcgc tattgataat ataagtcagt acaacatcga
    28021 tgtggccagg ataaagcttt ccagagttgt gaaactggat cccacatttg agaaagccaa
    28081 gattcttctg gataagataa attccttagg aagcgacaaa tccttaggcg acaaaatgaa
    28141 ggaagccttg gacaaaacag aggataaaga taagtccaaa ggtaaaaaag caaccaaaga
    28201 aaaaagcggc ggtaaagtat caataaaaac tttctttcaa tcgttgttca caggaagacc
    28261 ttcagttaaa aagaaatccg catatgcggc aaaaagtaaa ggtaaaatga ctcctcttaa
    28321 gttcattaaa atgcagatgt acattatcat aggacttatt gtagttattt taatactggt
    28381 agcggcaatt cttatcatga agaagaaaca cgatgattat atagcggcgc aaccggatct
    28441 cggcagtaaa ataacccagc tcacacagga aaagcatgaa cagtcggcat tgtatcaggc
    28501 aaccgttctg caatatgaag acaggataaa aagactggaa gatactctga acaattccga
    28561 ttccgatacc aataaataca aacatcaatc ggaaattctt ctgcaacaga aaagattgta
    28621 tcaggggaaa tatctttcgg tttcacaaaa atatgatgaa gccttagtcg ttcttaatga
    28681 gattgatacg gcaaaagcgg agttctccga tgcggactat gctatgtaca atgaaaccgt
    28741 tgcccaatgt aaagctaata tagcaatttc tctatataat tccggcaaca aactgtatca
    28801 ggatttgaaa tacaaggaag catacagtaa cttttatgat atatggcaaa gttatcccga
    28861 ctatgactcg gttaaagtat gggagaagga caatctctat tccaacatgg tctacgatgc
    28921 cgtttataaa atgtccaaat gcgccttcga gataggtgaa tataacattg ctataaaagg
    28981 atatacattt gttgaggaca gcctttcgga atttgccaag gcaaacagag aaggtttgat
    29041 atatcacagt gcaaaggcat atgcgggaat agaagactat gtaaaagccg aagaactttt
    29101 taagaaggta atagacgaat atcccgcttc caatcttgca atttatgcaa aagacggact
    29161 tgccgctgtc cgaaagaaat taggaagata gcagaggtat gctttgaaca gtattgattt
    29221 gtttgcggca atcgccgata caaagataga acaaagaaaa gaatatatgg aagcaaagca
    29281 accggtaccg gtaccggatg ttttgtttga gtgtgtcaat attgttaaag acaatgatat
    29341 ttgtaaagat aacaatgaag gcaaattgat tgataaaata aaagataaat attcttttcc
    29401 cgaggagcaa tcgtacgagc agcaattaaa gaattactat gaaggtcttg agcaattaaa
    29461 agcgcaatac gcaccttttt tgaaaagaag aagtaaacat gcaataaaaa gagcgagact
    29521 ggagtttgag aatttcgatt tccgttttga gacacgggag gatattgacg atttctcagc
    29581 cgttttggaa ggaaggggta tatgggaaag cataacaata cctcattacc acggtcctac
    29641 gggtaaatgg gtaacatatt accggacggt atttgattac aaaaaagaca acttaagaga
    29701 ctatatcgta ttcaaaggtt ccgactacat taccggggta tatgtcaacg gccgacatgt
    29761 actgtcgcat gaaggtttct tctttgcatt tgaagaagac attacggaat atcttaaaga
    29821 gaagggaaat gttcttgtag taagagtgga aaacgatatc accatgttag gggaaaacgg
    29881 cagattgggt aaaaagatat atgccgcaac cggtatcggt tgggatgacc cgaaggacgg
    29941 atggcatcat tgtccccccg gaggcggtat ttttgataag gtatatattg aaaaaagaga
    30001 aagtacattt atttttgatg cctgtgttta tccggatata gacaatcaaa cggcaaagat
    30061 aaaagtggta gttaaaaatg ttgataggta taagaaagat gttaagataa atatttctgt
    30121 tgaaccgttt aattttgacg attgcttcag atataaaggt gagtttcaaa gaaaaataca
    30181 tgaaggggaa aaccactacc attttgacat caaaatagaa aaccccagat tatggcacag
    30241 ttttactccg tatctatata ccgtgataat agaatcggac aattccgatc aatatgagca
    30301 gaatttcgga atgcgtaaat tccatatgga tacggagaat aaaccgtacg gaacgcttta
    30361 tcttaacaat accgagattc tgttaagagg tgcaaatgag atgggccatt tgcagttatg
    30421 cgtaatgaat gatgattatg agcagctcat agaggacatc cttatagcca aatactgcaa
    30481 tatgaattat tacaggatta cgcagcgacc ggtgcaatcg gaaatatatg aatactgtga
    30541 tatgctggga atgatggtac aggcggattt tccgttattc ggtcagcttg caagaagcca
    30601 gttttatgaa gcggtaagac aggtaggcga gatggaaagg catgttagga gacatccgag
    30661 cgtaataatg gtttcctaca taaatgagcc taccgatcag ttcaaatcgg acaatcagca
    30721 aataaatctt aacagagcgg aactggagaa ttggtttgag atatgtgaca gagtaataaa
    30781 gcaggaaaat ccttacagag taattaaaag ggtggaaggg gattacgatc ctcctacctt
    30841 tgaaggctta tccgatttcc actgctataa catgtggtat acaaatcatg ctctaccgat
    30901 aggagattta tacagaggtt atctgcccgc tataagaaaa gattggaaaa cggcatgcgg
    30961 agagtacgga tccgagggtt tggataatct tgaccttatg aaagaaagat acccgaaaga
    31021 atggcttccg aagaatgaag aagactgctg gataccggac aggatagtaa gagcacagac
    31081 caattccatg catggtgact ggttctatga gcaggataat ataaaagact ggatatattt
    31141 cagtcaactc catcaggcaa aagctgcaag tattatgact ctggcattaa ggagaagaag
    31201 cgattatgtg gttcagaccg cattgcacct tctgattgac gcttatcctt cggggtggat
    31261 gaagaccgtt gtgggtgtgg acagagtgcc gaaacctgca tatttcgctt ttaaagaggc
    31321 cctgatccct cttaaagtca atttgcgatg tgacagaaga acatgttata caaacgatgt
    31381 attgaatgcg gaatgctgga ttttaaacga taccgccgtt gattacagcg atatgacaat
    31441 aacagctgtg ttatatgacc gacacaaaaa ccccttgaaa acttacaaat tgaatacttc
    31501 cgcaaaaggt tgtaaatcag ttcccgtcgg aaaaatacgc attgacacat cggatattgc
    31561 cgtaaaggaa agagaagcat tttctttagc ggtatttatg gagcatcagg gcaaggtaat
    31621 tgataccgat gtgtataatt ttgatatctt cgaaaataat tatgaaagta tcaatgccta
    31681 tcctttagga atgcttgcga aacaggttgt aaatacaagc cccgctttta agcaggcaga
    31741 taaagaaacg gcagatatta taattatttc cgataaaaaa catttaaagg aagcggaagc
    31801 gatcaaaaaa agcataaata aaccaatctt atttattctg aattcctgtc tggagaattt
    31861 taagtttaac cgacacactt atgagatgag cgacagaagg atgtggaaag ccacatattg
    31921 tccgatcaat caaagccttt taccgggtta tagatgggat gatttatctt tcctgtatga
    31981 ctccgatacc gataagatta attttgttgc ggaatatttc atacagtgtg ataaggtaga
    32041 gaagatgtat gtctttaact ataaaaaacc cgccttcggt gaatcggctg aaggtcgtaa
    32101 agaaaaaaga ccggtactta tgcataatga ggacggaata tttacaactt tgaatttaaa
    32161 aggcagagta ggagttaatc cggtttcgga taagatactt tatgatgtgg tgaggggatt
    32221 gttatgagaa ttgctataag gataatggct gtagtttttg caataatatt gtgttttgtg
    32281 ttcctgttaa gttcgatacg gtatgtggct tttaatgaga agctttacag gaaattacag
    32341 attaaacatg aaatagcccc gtttgttctt atgactcagg aggagttgga tgatattacc
    32401 catgagatta taaaatacat gaaaggggac agaaaagacc tcgttatata tgacatacag
    32461 gaaggagtta aaagagagat attcaatgaa cgggaaaaaa cacatatggt ggatgttctt
    32521 aaccttttta ctgtttccga gtgggtttta gtgataatga tatgtttact tgcaatcata
    32581 gttattgcgg gatttctcat ggatatggat acaatgagat cgattttctt aaaaaccgta
    32641 atgataacca tggtaataac cgtactgctg tttataatgc ttatcttcta tatgatagtg
    32701 gatttccaca gtttctggat gttgttccat gagattctct ttacaaatga tttgtatctg
    32761 ttggatccct ataccgattt gttggtaaat ataatgtctt tggacttctt tgtgtcaata
    32821 tcctattatg tcagtctctg tttcggtttg ctatttttag gaatgtttgc taccattggt
    32881 ggagtaaaac tgatgaggaa ggtgataaag agtggacagg aagtttgacc gtaaaattga
    32941 tataaccaca attccgggta tacgtacata ccgtaatact cttcaactta ttcttctgaa
    33001 agttcttaat gatatttttc ctcacagaaa agtaaagatt gagtattcga taaataaagg
    33061 aagtttctgt acggttgagg atataaagat aaataatgag catgtggaag ccattaaaaa
    33121 aggtgttcgg gaatatatcg ataaggatgt accgattaag gaattacact gcacaagaca
    33181 aaaagccatt gagattttta gggaacgtaa gatatatgac attgccgagt cattggaata
    33241 tgtcaataac cccaaagtac tcatatacaa tctggacggc tttatatcca tcttctattc
    33301 caaagtggcc gaaagaacat catatgtgga tttgtttgat ataagggcat atgaaccggg
    33361 ttttatctat ttctatccga cacgtcataa accgtatgag ttaccgcagt atacggaaca
    33421 gaataaactg gcagcggtat tcggtgagtt taacaggtgg ggagatgtac tgggcattcc
    33481 cgatgtaacc agcttaaaca gggtagtaag aagcggttcg gttaacgata taatacttgc
    33541 tacggaagct cttcaggata tcaaattagg acagatttcc cgtgagataa gcgaaaagca
    33601 taaaaaattc gtcatgatag ccggtccttc cagcagcgga aagaccacct ttgccaacag
    33661 gcttaagatt catctgattg ttcacggagt taaagcaaag ataatatcac tggataatta
    33721 ctatattaac agagcggata tggtgcccga tgaaacgggc aaagtggatc ttgaatcggt
    33781 agatgctctg gacatatgca tgtttcagga taatatgaaa gaactttcaa aaaaaggaca
    33841 cacaatgata cctgtttttg attttaatac ggcgtccagg aaagaggaac ttataccgct
    33901 ttcattgagc gattcggagg ttcttatcat agaaggtata cacggactta atccggtcat
    33961 gacaaaagga atcgaggaga aatatattta caaaatatat atcagcgcat taactactat
    34021 taatatagac agtcacaaca gatttgccac tactgataca agactcatac gcagattaac
    34081 cagggattac gaatacagga attcctcggc atcccgtaca ttgaatatgt gggaatcggt
    34141 cagatcgggt gaggataagt atattttccc gttccaggaa caggccgatg tgatattcaa
    34201 cagtgcattg atgtacgaac acggattgtt aagagtattc gcggaaccta tattgaagaa
    34261 agtcaagaaa gatgacccgg catatgatga ggcgaaaaga ctgttggata tactgtcggc
    34321 atttgaaaag attgattccg cagcggtacc tcccagttcc atattaaggg agtttatagg
    34381 gggctcctgc ttccattaat caatatttag ctatacactc tatttctatc aaagcatcct
    34441 tgggaagcct tgccacttcc acagtggaac gggcagggta gtcggatgtg aaatattcgg
    34501 catatattgc atttatgacg gaaaaatcat tcatatcttt aataaacaat gttgtcttaa
    34561 taatatcgct catatccaga cctgcacctt ttaataaggt tgctatgttt ccaagcaccg
    34621 tatgggtctg cttttccact ccttccggaa tctttcccgt ggcaggatca acagggagat
    34681 tgcctgatac aaaaagcaaa ttatccgctc ttaccgcctg agaatagggc ccaattgcct
    34741 gagggcatcc ttctactttt ataatcttct tttttaaatc taatctattt ttcatatcaa
    34801 taccgccttt aaataatgta tataattcag tacccctatt ttatcatata atcataagtt
    34861 aaaagatggt ataattcgta tatggatatt ttgtctgata taaaacataa ggatattcta
    34921 caggtctcat tcggaaaagc aactgcggat atgagtaaag ccggaataag aagagtgaat
    34981 gacggctttc aactggagta tttttccgat aataaagctt ttcatatcaa ttttgatgga
    35041 agtaatctat ttgagcatat atataagctt attacgacta atttcaaaga atgtactatc
    35101 tttaccaaac agaacgacat agttgtattc agaaacagga aaaacgaact tatcataaaa
    35161 aatcataagc ctacaaagtc agttgtaaat acaagtcatg ataaacagaa gaaccatatc
    35221 ataaaagacg gaaccgatgc accgtttctt tattatctgg ggatatctga tataaacggt
    35281 gttgttaccg acaaagggcg cagaaaattt gtgcagatta acagtttttt gcagatattc
    35341 gaccacgcat ttgaaaaact gtcatttgat aataagcaac ttaaggttgt ggatttttgt
    35401 tgcggtaaag gttatctgac ttttgcatta cacttttatt tgcataatat taaacatatg
    35461 aataccaata taacaggtat agatttaaag agtgatgtta tagtaatctt aatgatattg
    35521 ctgaaaaata cggtatgaca gatttaactt tcatatccgg cgatattaag gcatatgacg
    35581 attacttgga tatagcggta ggacttcatg catgtgatat agcaactgat atctttttga
    35641 gcagtgctgt aaggaacaag gcaaaactca tcatatcggt tccgtgctgt cagcatcagt
    35701 tatttaagca gatagataat gaacaactta aacctatgct ttcatacggt ttacagaaag
    35761 acaggtttac cgaaatgctg acaaatacat taagagttct ggctttgaaa tccaaaggtt
    35821 acagtacgga tatggtggag tttgcacctg ttgaacatac aatgaagaat gttcttatca
    35881 gagcaatata taccggtaaa ccggatgaaa atgcaaaaga ggagtataat cgtttgaaga
    35941 caatgttttc agtaacggat tttgaaggag atactatatg agatgcctga ttaactgttt
    36001 ttcaccgaca aggaacacat ataacttgtg tgaagatatg gctaaaacac tgagaaacaa
    36061 cggatatgcg gtagatatat ttgatgttac aagacgtgca ataagagaga gaagacaggc
    36121 ggtggatatc gcatatgatt tgtttgtcat agcttttccg gtatataatc agtccattcc
    36181 cgatgcgtat gcggaatatc ttaaacatct taaaatcaat gcggaaaatg gtgctatcgt
    36241 agctacatac ggttccgtta ctataggtaa cgctttatct caggcaaacc atattttaaa
    36301 ggaaaaaggt atatcgatat acggagctgc tgctatacct gccgaacatt cctattcaag
    36361 tgcctttaaa gattacggta tatactacga ttattccgtt gaattggaga atttcttaaa
    36421 aaaagttatt tacaatgtga agaataaaaa gcatgttgat ataaaggggc agtttgcggt
    36481 agcggaactt ttcggtcaga agtttttagg aaaagtttca ataaagatac ctcgcgtaga
    36541 taagaataag tgtgtacggt gcaataaatg cataacggca tgtccgaccg atgcaattga
    36601 taaagactta agtatttcgg ttaaacaatg cataagatgt gcagcttgtg tcaaggtatg
    36661 tgaatttggt gcaagactct gcactttcaa taatcccata gcaaaatcca ttgtatccaa
    36721 gggagtaaaa aacttaggaa aacttataga tattacataa acgatttagt cattatcccg
    36781 gtttcacaag gtgcaacatc ggtaaatccg gaattgtgcc tgatttcctt tattgcatct
    36841 tctcgtgtca aagaacctaa attaacggca acggataatt ccagtgcgga gaaaggtatg
    36901 atatcgcttt ccatggcttt gaaagactga tactgtgaaa aatccttaca ccgctctatt
    36961 ttacagctgg tatgaagtcc tgaatctgta ttttccgaat caatccatcc ggctttatct
    37021 ttgagtaatg cctttatatc cttccataca tatccgtcgg ataaaatatt attcatacaa
    37081 cctttaatta catcgttaaa atcgatatga ataagggcag gtatgttgcc gtcaagtgag
    37141 gcttgttcaa cctctttccg aaatgaagta atcagaacat ccgcctcttt caatgcttga
    37201 tgtatgtttc ttatcatagg atttcttact gttttagcgg atataaaccg aggttgcccg
    37261 aaggccaatg tttccaaaag cagaagcatt tccatccgac ctttttttag cggcttcaat
    37321 cctttaaaaa ttctcaccat gttatatata acccgaaata gcatctgaaa taacgggtta
    37381 tagaaagttt ttggtgataa gttgttgaat atgagattta tgggttgagc cggttcgttt
    37441 cccgaaacca catactttac acccgaatct ctcaattctt tcaaaaacag gggataggct
    37501 gcaaagggac atatacatac atttttcata agttcatagg atttcttgta tatggccgct
    37561 tgttcttcat cattcaatgc tttggaaata aagtcggtat ctgtgaatat ttcttttgca
    37621 ttattcatac ttttaattgc attatccgac atgaaaggaa tatcccatgt aagagcaagc
    37681 accttcagtt ttaacacatc tgcaaaatag tataaaagaa aagtggagtc tttacctccg
    37741 gtatagaaaa gagcgacatc atattttgcc ttttcattgt gttctatggt gttaactacg
    37801 ggtaagacat cttttatatt cttaaacgaa ggcatattgg tacaaacggg gcataatccg
    37861 ttgtcatcaa actctatacc gggtatcaca taatcgtttg aacagcagtt tttgcagaaa
    37921 aatgcattat ctatagtatc ggggacggtt cttttggaat gtacatcgac aattatcttt
    37981 ttcttaatca attgctttat ctgactattt atcttacact ccgacaaatg tcttattccg
    38041 tcaaaagact gtattatttt cgcagtggaa gccgatatgt ttaccgtatt tttaaatgta
    38101 tacggctttt tacgtaagcc gtagtatttt aatatattat cttttaaaat ccatctgttt
    38161 tccagtacgg gaatcatgtg tgtcctcgct gatttgatat tacagataca ttatacctga
    38221 atcaggcaga atatatagcg gatattgtgg ttcgtaatga aaatgaatat aattttaatg
    38281 agggtgatgg aaatgatgaa ttcaaaagag gtagttaaag cgactttaca gtttaaatat
    38341 ccggacaggc tggcagtgga ttttccgccg gaatacggaa cggatttcaa gtttatcaat
    38401 atgagggcaa atcccgatta cagaccctca aaaggcaaag atgaatggaa tgccgtatgg
    38461 gataatatcg gtatatccaa actgggagaa gttaaggaat ttcctcttaa aagctgggat
    38521 gactttgaaa gcatgcatat acccgatgta acggaggata gaagatggga aggcgtaaag
    38581 gagaatattg aaaaccacaa agataaattc accatgggtg tgggaatttc catttacgaa
    38641 cgagttcact ttctgagagg attggaaaat acctggattg atatatatga gaataccgat
    38701 tatttgtgtc agcttattga tatcttaacc gatatgaatg tgcatattgt ggaacggttt
    38761 gccagttacg gcgtcgattc gctcatattc gccgatgatt ggggattaca gaactcttta
    38821 atgatttctc ccgacaaatg gagagagata tggaagccga gatacaagag gatatataaa
    38881 gcggctcatg ataataatat gtatacaatt cttcacagtt gcggggatat aaggagtatt
    38941 cttgacgatt tgatagatgc cggtctggat tgcattcaga tggatcagca gcagaatatg
    39001 ggactggaat atctggggaa atacaaagac aggattactt ttttctgccc ggtggatata
    39061 cagaatatat ttgttaaagg ttctttggat gagataagaa gatattgcca taccatgttt
    39121 catgcactgg gaagtaaaaa gggcggattt atggccaaat ggtattccga cccggtagga
    39181 gcaggacaca gaagagaagc aattgatgtc atgtgtcagg aattccttaa gataagtgcc
    39241 gatgttttcg gtcattcaat agttaaatat tagcattttc cttatactaa ataccactta
    39301 tggtcgtaaa acgaccacct gtcaggaatc ggtggaaatc gtttttttgc tttagtatat
    39361 tgtaactgcg gagaagggag gggtgattaa tgcagataga aataaagata gacgaagact
    39421 gtaaagaacc gaagattatc gttgtaacgg ataagatgac agaagagatt aatgaagtca
    39481 ttaaaaaact ttcggaacag caaccgcaga taattgccgg attcaaagag gagattgtta
    39541 aggtgttgga gctttccgat atttaccgta tatatgcttc aggcggtaag gtatttgccg
    39601 aaacaactca cggggagtat acgctccgtc tcagacttta tgaaatagaa gagaggctgg
    39661 acaataataa ctttgtgcga atatccaact cggatattat caatctgaaa aaagtaaaag
    39721 gttttgactt aagtctggca ggaacaattt gtgtaatact ttccaacgga acggttacat
    39781 atgtatcaag gcgatttgtc gccagaataa aacaattatt aggaatatga ggtgataaaa
    39841 tgaaaaagaa gattattaat cgcggtttgc tcggttttcc tctgggtatt gcaataggct
    39901 atatagtttc catacttgtt tcgctatatg caggagacgg aatgttccat tttgcaaaac
    39961 cggaattggt ggaaaaagtg ggcagtgaac tcaatgccgt tatattacag acagttttgt
    40021 cgggaatact gggagcagga tttgcaatgt cttccgtcat atgggaaata gaaacctgga
    40081 gtcttgcaaa gcagacaggt atctattttg ccgttatatg cttagttatg tttcctattt
    40141 cttatgttgc aaaatggatg cccaacaccg taggaggagt tatatcttac attgcgatat
    40201 ttttaggaat ttttatcgtt acctggctga tacagtattt tatttggaag agtaagatta
    40261 aaaagatcaa tgaaaaagtc aaggatgtag aaaaactaag taccgagtaa aaataactga
    40321 ataaagaata agcaaaaaga agcggcgtgg tcataaagat tacgctgttt ttatagagcc
    40381 aaactgaccg attcggaaag tggctttatt aatatttcat atgattttgc tcaataagtc
    40441 tttttgtctt ttttccccac attccgggaa gttgcacggt acggtttttc acggtctcta
    40501 tgatttttga gtagctgttt atgaaagtct cttctttctc cgagcacttc tttgaaaaat
    40561 cggcaaattg ttctactgtt gcccctttct tcagcaagga ctcatagcct gatataaaat
    40621 cttcttctat ctcttttacc tgcttgatat gggaatcggc attcgttaag cctgcgaata
    40681 ttgctctgtt taagtactct ctttttaacc aataattcag gctatctttt tcatctaaga
    40741 aaaccggggg attaatatgc ccgaagaaga caggtttgaa taccgataag cagggggtag
    40801 aggagcctgt tacccagatg accgggatat cttctctgaa atctactacc atacttccgg
    40861 ttgtatgatc tccgagaaaa ctctggtgca tacaaacaga ggaaacactt ccttttgtgt
    40921 atagtttgtg cctgtcatct gtatcatgtg ctcttaatgc gccaaataca tcccttatac
    40981 cctttgcatc ttttatggca ttgcaggcac agttctgtct cttcattgaa ccggcaaaat
    41041 gagtaatgag attgttagta tgtttttttg caaaatcatc ctcttcaata gtgatacggt
    41101 tggaaatatt accgtaacct tccagcttct ttgtcttgta ctcaagtccc gaagactcta
    41161 aaatgtatgc ctctttttta tccgcaacca gaaaactgtt gtcgtaataa aacgtcttat
    41221 caaaggtaca gttacctccc tgaccgaacg cagttaatgc tttttttatt tcctctatac
    41281 cttcttttgc attttttgct ctttccaatg caatccttag aatatccatt cccagtaatc
    41341 gctctgtcct tttacctttt gatttggtaa aaagcgcttc gtttccgata gcaacaccgc
    41401 actcgttaat gcccatttcg gctccccaca tccaatgagg gcgaaccaga aggacagaat
    41461 atttcttttc ttcctgcggg actgttatat atgtacactt gacgggttga ccacccgttt
    41521 tcccgccggg aataaactgt accagattaa tctcgttaca agatctgtcg ctgttttttg
    41581 caaaaattcc gcctttttca aaggacttcc acatagtatc acacatatca attctcctaa
    41641 ctctgtaccg ctatattata tcatattcag tttgtggaat aattattata cattaaatca
    41701 aataggcaaa gaataacgga gcagtcaaaa gatatagctg ctttctgcat atgagatatt
    41761 ttgccagcaa tcagaaatat aaatttataa ggtctgcttt atagacaaaa aactgcatat
    41821 aacgggatag atttaatact gttctcatat ccgaagtttt ttgtcgaaaa cctgatagca
    41881 taatccggat tgtacttatc tgcaaaaaca gacaaacttc tggaccttac attatctgct
    41941 gattttacct ctatcggaat aattgaatca tctatacgaa ttatgaaatc tatttccgct
    42001 gttccgcttg actgccaata attcagcgga tatccggcag caaccaattg ttgagcaaca
    42061 taattttcgg cgagcatacc gcgtgctcta tgcgaagata tatttgatat cacatcttga
    42121 ggttgaattc ccgctttata ggagagcagt cctacatcgg agtaatatat tttaaacgaa
    42181 tcaatttcct gataaacgga taaaggatgt tttgcttccg tagctttaat actttccagc
    42241 accacatttg cagcttttaa ccattgtaag ctaagctcat agtctttagc cctggcagaa
    42301 cttttaataa tattgtaccg gaattttgtg ttttcctttg caagttgtgc aggtaaggta
    42361 ttgaaaatat ccgtactttt tatagtaata ttcggggttg agtattttgc catgtctgca
    42421 atatatgcgt tggcaatatt ggcttgttga gctttaacta cattaaaatc atttttctca
    42481 agatatgttt taactactgc cggataaccg ccaatcacga gatagctgtg ataaaggctt
    42541 aatgcttcct catgatatac tgccgaaagc ggtttaaatg acgaaaatga ttcctttatt
    42601 ttttccagca aaagatcttt cccgcatgca tataaaaact catcaaatga catcggatac
    42661 atagtaacca tatcaacttt tcctaccgga aatgaatatt tgccacgatt tatcgcaata
    42721 ccaagcagac tggcggcagc aataatattg tactgtggag cattttcaca aaaatatttt
    42781 aaagaattga gtgccatttc acatgcttgt atttcgtcaa aaattatcag ggttttctca
    42841 ggtattatag cttgtttgga ataataacta agttcttgca caattcgtgc aggttccagg
    42901 tcttctctga aaatatcatg aagcagtctc atatcacctt caaaattcag gtatacgaca
    42961 ttttgataat tattttttcc gaactctaaa catgaataag tttttcctac ctgtcttgca
    43021 cccataagta acaaaggctt tcttgaaccg gaaactttcc atttgtttaa tttatcttct
    43081 atcattcttt tcatagtatt caacctcgat aatcattata ctcctgaata tacagaaaaa
    43141 tcaatacttt taggccataa gttagaaaaa tgttatgtat aacacatttt tatcaaccaa
    43201 aaatgttgat gatgggaaca taaaattaac ttaattgtaa aaaacatatt ttgaaaagtt
    43261 tgaaatttaa aaaaatgagg gtctaaatat acttaaagac cctcttgcct ggtaccggcg
    43321 gtgggacttg aacccacaca tagtctccta taccggattt tgagtccggt gcgtctgcca
    43381 ttccaccacg ccggctgata cttttgtaat attaacatat taggcaatat ttacgcaaca
    43441 ggtttgttat aaatgttata atatcattaa gtcgttggta tttcggcgga aaagaggtaa
    43501 gaaatgaata aagatatagt aaataactac acattgcttt gcgattatta cgagcttaca
    43561 atggctaacg gatattatat agcggggtat aaggataagt attgctattt tgatatgttt
    43621 tacagaaaca atcccgataa cggcggtttt gccattgcat gcggattgga ccaggttata
    43681 gagtatatac agaatcttga gtttacagat gaagatatag cttttttacg ttccaagaaa
    43741 tgttttgatg aaggtttttt gaaatatctt aagggtttta aatttaccgg tgatatctat
    43801 gccgttcccg aaggcacggt ggttttcccc aacgaaccgc tgataacggt aagagcaaaa
    43861 acaattgagg cacagatttt agagaccttt atacttctta ctgtcaatca tcaatcgttg
    43921 atagcaacca aatccaaccg tatagtccgt tcggcaaaag ggcgtcctat aatggaattc
    43981 ggagcaagaa gagctcaggg aacggctgca tcggtgctgg gagcaagagc ggcatatata
    44041 ggcggatgtg ccggtacatc ctgtacattg gctgacaaaa tgtacggcgt acccgcaggc
    44101 ggaaccatgg ctcattcatg ggttcagatg tttgataccg aatatgaagc attttatata
    44161 tatgccaaaa cctatcccga taactgtgtt ttactggtag acacatataa tgtgcttaaa
    44221 agcggtgtac ccaatgccat taaagtgttt aaagagttgg gtattaagaa atgtgcaata
    44281 agactggact cgggagacat cacatatctt tccaagaaag ccagagcgat gctggatgaa
    44341 gccggaatga ccgaatgtaa gataacggca tccaattctc tggatgagta tattatcagg
    44401 gatgttattc atcagggagc aacattggat tccttcggag taggtgagcg ccttataact
    44461 tcggaaagcg atccggtatt cggcggggta tacaaacttg tggcagtgga agatgataag
    44521 ggaaatgtta ttcccaagat aaagattagt gaaaatccca caaagattac cacaccgcat
    44581 tttaaaaagg tatacagaat atattgcaaa gacatgttaa aggcggaagc ggatttaata
    44641 tgcgtttacg atgaagttat cgatacaagc aaacctttaa ccatatttga tcccgactac
    44701 acatggaaga gaaagacatt tgaagatttt actataaaag aattattggt accggtattt
    44761 aaaaacggtg aatgtgtata tgaccgtcct tccattgaag agataagagc atattgtgca
    44821 aaacagattg acgaacagtg ggatgaagtt aaacgttttg aaaatcctca taactactac
    44881 gtggatttat cgcagaagtt gtgggatata aagtatagga tgcttaacag agaataaaac
    44941 ttttattaat tagtttataa ggagaaaact tatgctgcta ttaggtgtaa cggaagataa
    45001 aggatggttg gctgcaacgt ttaatctgac atacagggta ggatgggaac aaattcaaaa
    45061 aggtgtcggt acggcttacc ggttttttaa gaaaacggag attctggttg atgataaacc
    45121 ggtgagtgtt acttcaggag aagatatcat gaaactggaa gaagccggaa gtatgacaat
    45181 ccgcgggtta tccacaataa ttaaagttcc gctcatgata actttctata accaacttca
    45241 gacggttaat gttgcgctgc cggcgcaaaa tgaggaattc agaaatacgg attatcagaa
    45301 attcaacatg tcgttgggac agtatatgga cagcattgaa ttggccatgt atcgttaaat
    45361 ttaccgaatt ttgttgctat aatcaaagaa aaaggttgac agtgaagaaa atattcgtat
    45421 aatctgcttt aagtaggacg ggaggacggt agtatgatta agatcattat tattgctata
    45481 tttatcgcta tacaggtggc tgtgggaatc tacagcgtca gaaagattaa gaacattgac
    45541 gattttttgt tgggcggaag aaaaatggga gcttggctct cggctttggc ttacggaact
    45601 tcctatttca gtgcggttat ctttataggt tatgccggtg gtataggatg ggaaatgggt
    45661 acttccgccg tctggattgg tataggtaat gcggttgcgg gcagctatct ggcatggaga
    45721 gtgcttgcaa aaagaacacg atccataacg agagaattgg acgcaaagac catgccggag
    45781 ttctttcaga aaagatatga ctcggaagct ctcaagattt ttacttcagt actgacattt
    45841 atatttctgg ttccgtatac agcttcggtc tataaagggc tgggatacct gttttccaag
    45901 ctctttcccg catcggtatc ggtttcgcag gcatttacaa ttaccgttat tataatggcc
    45961 gcttttactt tgatatatct tgttctcgga ggatatatcg caacggcact taataacctt
    46021 attcagggca caataatgat tataggtacg gttatcattg ttgtatatgt tgtaaaatca
    46081 cccgaggtaa acggatttgc caacggattg gcttctttaa acaatattaa agaaggttta
    46141 ggcacgataa caggtcccga tccctggaaa cttctttcat tggtgttgat aacaagtttc
    46201 ggagtctggg gactgcctca gatggtgcat aaattttatg caataaaaga tgaatcttct
    46261 ataaagaaag gaacaataat cgctacggtt tttgctgcgg ttataggtat aggagcatac
    46321 ctgaccggct cgttcggacg acttttcttt tccgagatac cgaacggttc tgtagatacc
    46381 attgtaccca atatgttggt taagatactg cccgatgctt ttttaggtat attcatggta
    46441 cttatactgg ctgcgtctat gtcaactttg tcatcactgg tgctggtatc cgcatcatca
    46501 atatcggtgg atctggtcaa aggatacata aagaaggata tgagtgaaaa gagtcagctt
    46561 actctttttc gaatactttg cggtgttttt gtagtgatat cggtaataat ggctcttatg
    46621 aataataacg caatagtaac attgatgaat ctctcatggg gaacgctggc aggatgcttc
    46681 ttaggtcctt atgttgtagg gatatacagc aggagagcgg ataaagtttc cgcttttgcc
    46741 ggtatgcttt ccggctttac agtaatgatg acacttgtta ttataaaagg gatgacatat
    46801 tccacggtag caggatgtat agctatggca acatccgtag ccgtttcatt catagtttca
    46861 aggttgtcgt ggaagaaaaa cattacagtt gaggagattt taaatgaaaa agttgttact
    46921 ggcgaatgaa gcggtagcaa gaggattata tgaagccgga gtaaaagtgg tatcggcata
    46981 tcccggtact cccagtaccg agataaccga agcgacatca aaatatgatg aaatatatac
    47041 cgaatgggct cctaacgaaa aagtggcttt ggaagtagcc gcatcttcgg caatagccgg
    47101 tgcaagatcc ttttgcgcta tgaaacatgt gggactgaat gtcgcatccg acccgctgtt
    47161 tacaacatcc tatataggag tcaatgccgg atttgtaatt gcggtagccg atgatccggg
    47221 aatgcattca tcacaaaatg aacaggattc aagacattat gccaaagcag ccaaggttat
    47281 gatgttggag ccgtccgact cgcaggaagc caaggatttt accaagaagg catttgaatt
    47341 atcggaacgc tttgatacac cgattttact gaggctttcc acgcgggtaa gtcattcccg
    47401 gagtctgaca actcttgagg atagagtgga tgtgccgctg aaagattatg taaagaatcc
    47461 gaagaagaat gtcatgatgc cagcaatggc taaagtacgg catgagatag tggaagaaag
    47521 ggtcattaag caaaaagaat acgcttttct gtcggaattg aataagattt gtataaagaa
    47581 taaggatata ggcttcatat cttcgggaat agctttccaa tatgcccgtg aagccctgcc
    47641 cgatgcatct tatctgaaat taggtattac ttatcccata tctgaagagt tattaaagaa
    47701 atttgcttca tcggttaaaa agatttatgt tatagaagag ttggaaccga taattgaaac
    47761 ggcatgtaag gcatgcggta tagaggtgac ggggaagcaa ctgctgcctg taaccggaga
    47821 atattcggtg gaacttttga gaaacaggtt attcggtcaa aagccgtata actgcatgga
    47881 aggctatgat atgagcaaag tgcccggcag acctcccgta atgtgccccg gatgtcctca
    47941 cagaggaata tttcatattt taaagagact taaactgacg gtaacgggag atatagggtg
    48001 ttatactttg gcaactttgc agccgcttgt cgctatggat acttctttat gtatgggaag
    48061 cggtattaca tcacttcacg gcttcaataa agcaagaggg gatgaatata tgaagaagtg
    48121 cgtgggtgtg ataggggatt ccacattcgt gcattcgggt attaccggcc ttatagacat
    48181 agtctacaac aaaggggtag gcaccgttat agtattggat aattccataa ccggtatgac
    48241 gggacatcag aataatcctc tgaacggatt ggatattcac aataatcccg ccaaacagat
    48301 tgatctggtt atgttggcaa agagtatcgg aatcgagagg gtaagaataa tcgatccttt
    48361 tgacgtggaa gaagcgatga gggttataaa agaggaggtc aatgcaatgg aaccttctct
    48421 gataatctca caaagaccgt gtgcattgct taaaacggtt aaatataaag gatgcgttac
    48481 cgtagacaga gacaagtgta ctaaatgcaa aatgtgtatg agtctgggat gtcccgcaat
    48541 atcttttaaa aacaataaga tagaaataga tacatcctta tgtaacgggt gcggtttatg
    48601 cgtcaatgta tgcagattcg gtgccttgaa aaaggaggag aagtaatggt taagtcaatt
    48661 gtaatagcag gggtgggcgg acagggtaca ctatttgctt ctacaataat aggagaagca
    48721 gccacgctga aaggttttga tgtaaaagtt tccgaggtac acggtatggc gcaaagaggc
    48781 ggttcggtta taacatatgt gcgttacggc gaagaagtaa tatctcccat aatacctgtt
    48841 ggagaggccg atgtacttat cgcttttgaa cagctggaag ctctgaggtg ggctcattat
    48901 cttaaaaaag gcggtattct cataagtaat acacaaaaga tagatcctat gcctgtaatt
    48961 acatcttcca tgcaataccc tgatgatatt atttctgagc ttaccggaag ggtagataac
    49021 tgtatcttca tggatgcttc ggtattggct gaacaatcgg gttctataag aacggtaaac
    49081 tcggttctga taggtcgcat ggcaagagat tccgacatag aaaaagaaat atggctggaa
    49141 gctttgaaaa agattgcaaa acccgctttt atcgatatga atataaaggc atttgaagcc
    49201 ggatacaact acacagtttg actctgtccg ttaaacttcc cgtttgctat atgagcaaaa
    49261 ctaaggtata attatggtta atgcatattg tatctttagc aaggggcgtt tatgagcatc
    49321 actgaaaagg acaagatatt cgaacaggat agattgaatg aggtctatgc caagctgtca
    49381 aaaaggattg aggaccttga ggtatcttta aaacaaagta ttgattcttt aagaaaaagt
    49441 aatcagatga tgtgggaaag cggaaagcga aatcccgcgg attttcatga tgccatagaa
    49501 aatatgcctt acctggaaag catcaaatac gactttttca aaaccgaaac aattaaaaaa
    49561 gaagtaagaa ggatgtatct gcttcaaaga agcatgtatt tcggcagaat agattttcag
    49621 gaacaaaatg aagaggatgt agagaacata tacatcggaa agttcagtct gaccgatgat
    49681 gaagggcagt atctggtttt tgactggaga gcaaatatat gcagcctgtt ctatgaaaac
    49741 gaattgggaa atgtgtatta tgacgctccc gacggtagaa tttacggtga gatgtctaaa
    49801 aagcgacaat ttgacatttt atataacaat atacatgcta tgttcgattc ttcgttaatg
    49861 attgacgatg atatcttaaa agatatctta agtaagtcca aagactcgaa gatgagtaat
    49921 attgtggaaa ccatacagaa agaacagaat aaagccataa ggagcactta cagtacaatc
    49981 atagtggacg gaccggcagg ttgcggtaag acttccatag caatgcacag agccgcctgg
    50041 cttatgtata aatataagaa tgaattgcag aataaccaga taatcatatt ctcgcccaat
    50101 gaaacattta acgactatat atcggaagta ttgccggagt tgggtgaaga gaatataagg
    50161 atgtctacat tcagaaatct ggcatatcat atcttatccg acagaagagt gcttatatcc
    50221 aaatatgtgg aattggaaga tgtgctgttg gataacgaat atattgatat tgatgtttta
    50281 agacttaagt attcagtaga ttttgcaaac aaacttaaga gttacacgga acttatctcc
    50341 gaaggtgact ttccttttaa agaccttatt gttgacggaa ttacgatttt tacaaaacgg
    50401 gagtttgaag acctgttcta taacgaatat tcttttaacg atgtaatgac cagactggct
    50461 aagataagga tagtgcttaa tgaacggata ttcccggtta tccgaaactt aagattggaa
    50521 tatgtgcgta aagttaaagc cgatgaattg gatatgtcaa aaggattcaa agagttaaga
    50581 gatagttgca aaaggattta tcaggctgtc gatatgtcgg tatcggcgga ttttgaaatg
    50641 atttatgctc aattcctggc gcacagctat tcggcaaaga taagttcgga ttttttgaaa
    50701 agacttcgtg acggttacat atatgccgag gacactgcac cgttaatcta tttgcgactg
    50761 ctattgggta ataaatacaa tttcagtgat ataaggcatg taataatcga tgaatttcag
    50821 gattattcct atatcgaaaa gcttgtcata tcattactgt tcggaaaatg tcatatgacc
    50881 ttactgggtg atatcaatca gaaaatcaat cactaccagg aaaaagtcaa cgataaggat
    50941 attttcaaag gtgccagatc ggtaaaactg gataagagtt atcgttccac atatcagatt
    51001 gcgaaattct gtaacgatat gctgaaagac aatcttgata ttaaatattt taacaggcag
    51061 gggaaacaac cggagatttt aaacttaaat actgccgact tggatgtaat agcaaatacg
    51121 gtaattaata aagtatcgaa gtacagagca tcaggtcatc gttccctcgc cgttataacc
    51181 agaactgagg catttgccaa gaaacttcaa ccctatctca aggatgcgaa aatcaacaga
    51241 gttaccgaaa agagtacaac ctatacatac ggtgcggtgc ttataccgtc ttatcttaca
    51301 aaagggctgg agtttgatgc cgtaatcgta ctggatacac aaaatgacag gtttaaaaaa
    51361 gaagaggaat tacacctctt ctatacctgc tgtacaagag ctcttcacga actcaccgta
    51421 ataaactaac ttaaatcata tccttacaca cttcataaac atgcttcata acggcatcat
    51481 ttccaagctc gttaaggcat tcatgacgca tatcttcaaa tatacgtgat gttacatttt
    51541 taatgcctgc attctgcaac cttgcaacaa gagcaagcgg tcctttggaa ttacctccta
    51601 cggggtcatc ggagccgcag aatgaatgta tcggaagttc tttcgggatt tctgctatgc
    51661 tttctttttt gtaaataacg gacaaaccct taaacagttc tttaaacatt ccgaccgacg
    51721 gaacatatcc gcataacgga tcctcaacat atttgtccac ttccgattca tttcttgaaa
    51781 gccagtcgaa ttccgtacga ttgggtttaa actgtgaatt gtatgctccg aaagacaatg
    51841 cattaagaat cccggacggt ttattcctcc ctataaacaa ctcctgaagt gcggcaataa
    51901 accttccggc acccagtaca agagaaggtt gttggtcatt ggttcccgat aatatacatc
    51961 cgtcaatgtt ggaggcatat cgctcggcat atgccagagt cataaaagaa cccatgctgt
    52021 gtccgaaaag aataactttc ttatcgggat gttgcttgcg gacatattcc accaatgagt
    52081 tcatattgct tacacactcg aaaaatccgt caacaggcca aatgccgaga ctttcggtat
    52141 atacaccgtg accgatatgg tctatacaat acaactgtac attatgattc ttcataaact
    52201 ccgataatct tgcatacctt gcggcatgct ccgccaatcc cggtgctacg accagtataa
    52261 aatcgggatt atccgttata tgcggcttaa acacaaaaaa ggcgccgctc ttacctataa
    52321 tagaaaaata gtctttgttc atttttgtaa tctccctcaa ctatattatc acagatgttt
    52381 aaaattacca agagttctat tcatgaggta tgagcagccc ttgaatgcaa tacggagtta
    52441 ttcattgatt tttaatctgt ataagagtaa aatactataa aacggaggac gtcattttat
    52501 gatccgcgac agggttgaga gtatttcact tgatgagatg cgtacaatac agggtaaaag
    52561 attggtagaa accgtaaaac atgtttacga taatgttgca tattacagaa gaaaaatgga
    52621 atctataggg ctgctgcccg gggatataag agggtatgaa gatttacata aacttccctt
    52681 taccacaaag gccgatctga tagaaacata tccttacggt atggtagccg tggacatgga
    52741 taagatagtc agggttcatg catcttcggg aacaacggga aaaagaatag ttgttgctta
    52801 tacaaaagaa gatatcgata catgggcgga tgtgatggcc aggtgttttg caagaatagg
    52861 cactgatgta aatgatgttg ttcaagtggc ttacggatac ggtttgttta ccgggggact
    52921 gggaacacat tacggtgcgg agagactggg tgccacagtt atacctattt caggcgggaa
    52981 cagcagaagg caggtggaat ttctcattga tttaaaaccc acggttatag cttgcacccc
    53041 ttcatatatg ctccatttat acgaagttat gaccgaagcg ggtgttaccg ttgatgacat
    53101 ttctttaaag gcgggacttt tcggagcgga accgtggagc gagaatatga gaaaggaaat
    53161 agaacgcctt tttaacataa aagcttacga tatatacggc ttaagcgaga taaccggtcc
    53221 cggtgttgca tcggaatgtt gtgcgcagaa cggtttacac ataaatgaag actgttatct
    53281 tccggaaata gtggataccg aaacacagca acctttagga ttaggtatgc agggcgagat
    53341 tgtttttaca accataaaga aaaagggtat gcctttaata aggtacagaa caaaagatat
    53401 aactaaatta ttccgtgatg attgccgatg caaaagaaca ttggttaaaa tggaacgtgt
    53461 aatgggcaga accgacgata tgctcataat aaggggagta aatgtatttc cttcgcagat
    53521 tgagagtgta ttattggagt ttagcagtgt tactcccaat tatcagattg tagtggacag
    53581 aatcaacatg atggatacac tggaagtgcg ggtggagatg aatgaaaatc ttttctcgga
    53641 tgaagtaaga aaaattgaaa tgttggaaag gaatataacc ggagcaattg agagtgtatt
    53701 gggtatcagt gccaaagtaa aattagcgga acccaaatca attgaaagaa gtgaaggaaa
    53761 agctgtacgg ataattgaca gaagaaagat ttaaggggtg tgaaatgtta gtaaaacaga
    53821 tatcgatatt tctggaaaac aaagaaggca gacttgccga agtgacttcg atattggccc
    53881 gagcaaatat taatatcaac gcacttacca ttgcggatac agcggatttc ggcatattaa
    53941 gaattattgt cgatgatgcg gagaaaacgg aaaaattact gagggagtac ggtttttccg
    54001 taagtgtgac cgatgttttg gcgatagata tcgataatgt gccgggaggc atagcacgtc
    54061 cgctgataat gcttcataag aacggaatat cggtggagta catttatgct tttttggcta
    54121 aagaagatga taccgcacat gtcatattta aagttaatga tattgagacg gcagtcagga
    54181 tactaacaaa gaacggattc aggaatacgg caaaattatg aaagcggaga taattgcggt
    54241 aggcacggag ctgctgatgg ggcagattga taataccgat gcaagttaca tgtcaaagag
    54301 atttcccgaa tgcggcgtgt atgtatatta tcataccgtt gtaggtgaca atcgggggag
    54361 attggctgaa tgtattaaca ttgcgctgaa aagagcggat gttgtagtgc ttacgggcgg
    54421 tttgggacct actcaggacg atataacaaa agagactgtg gctgaggttc tgggactgaa
    54481 attggttttt catccggaaa ttctggagaa tgtaaaagaa tacttcaata caataaggca
    54541 taacaggaaa ataaatataa tggcggcaag caatgagaaa ctggcatact atcccgaggg
    54601 ttgtttaata ttgcccaata ataacggaac ggctccggga tgcattattt ctcattttgt
    54661 tgagggagcg gataaaacaa taatattgct tcccggtcct ccttgggaac tgcaacccat
    54721 gtttgataat tatgtcgttg attattttaa gagtaagata caggaaggtt tgaaatcggt
    54781 atatcttcgt atgttcggca taggggaatc ggctatggaa tcggttattt ccgatattgt
    54841 atcaactcag accaatccga ccatcgcccc ttatgcgaaa aaaggagagg taatgctgag
    54901 agttaccgcg cggtatgaga agggcaagca ggatcccgat ttaattctta aaccgatact
    54961 ggatatattg cacgaaaggt taggggagta tatttattcc gataaagatg ttgaactcaa
    55021 ggtcgttact gcaaacttgc ttatgagtaa gaatattacc gtatcttttg ccgaatcatg
    55081 tacgggaggg atgttatccg aattattgac cgatgttccc ggaatatcaa aggtgttttt
    55141 aggttcggtt gtggcctatt caaacgagat taaacaatcc gtgttaaaag tcagcggtaa
    55201 tacattgaaa aaatacggag cggtcagcga gcaaacagct aaagagatgg cacaaggtgt
    55261 acggctgctg tataatacag aagtcggtat ttccataacc ggcattgcgg gaccttccgg
    55321 agccgttgcg gataaaccgg taggccgtgt gtatatcggg ataagtaccg agcaggaaac
    55381 attgtcttat gaatacactt tcagcggaga cagagataag gtaagatatt tttcctgcct
    55441 taatgcactt aatatattgc gcttgtatgc actttcgaaa ggatgagatt tgaaagagat
    55501 aagatttaaa agtactgttt tccttataat ggcttcggtc ctgttgagca gaataatggg
    55561 atttgtgaga acagccctta ttcccgtgaa aatgggaggg ctttccagag ttaccgatgc
    55621 atacaacgcg gcttttcagg ttcccgattt gatgtattcc atgttgatag gcggagccat
    55681 agcttcctct cttattcccg ttctggcagg ttatgtggca aaaggagaag aaaagaaagg
    55741 ctggagatcc gtaagttctt tcatgaatct ggtctccatt ataatgtttg tcgtagtaat
    55801 attatgtgtt atattcgcaa aaccgctggt aaacatgctg gttcccggat atgatgcgga
    55861 aacactggaa ttaacggtac agttgaccaa gattctgatg cctatagcct tttttatgat
    55921 gcttgccggg ctttgtaacg gaataatgaa ctcatacaat gagtttataa taccggcatt
    55981 cggaccttgc ctatataatt tagcctgcat tataagcata gcattattca gcaatgataa
    56041 tgttgcggat aattacggag taaaaaaagt agtactgggt attgtaatat gtgccgggct
    56101 ctattttctt ttccagttcg catttacctt caagcatttc aagaataatt acacaaccgt
    56161 aatcgcatat aaagagccgg atttcaaact gttgatagcg cttgcgatac cttcattgct
    56221 gacatccatg ctggggcaga taagtctcat aatatccaga tattttaccg cattctttga
    56281 tgtaggctcg gttaccgctc tggaattggt caacaagaca tggcagatgc cgctgggtat
    56341 tattgcgcag gcgataggta ttgccatgct gccttcatta gctgcggttt acgatgccga
    56401 taagaaaagt gaggtggtct ataagattaa tacctcctta aggttggtta tattcctggc
    56461 cataccttca gcggtaggat tgtcggtgct cagtaaaccg ttaatacagg tacttttcaa
    56521 ttacggaacg atagaacagg aaggtattac attagcagcc aatttattga taggctactc
    56581 tctggcattg gttatgcagt gtattataac aatagttaac agagtatatt tttccaccaa
    56641 aaattccatt attcctttct ggggaagttt aatcggtgtt gtgttcaatt acctcattgc
    56701 ttatttactg ttgaaactga ccgatgccgg aattttcagt gtgcccgtag catattctct
    56761 atcctccgta ctcaatcttg tatttctggg actacttttc aaaacaagga ttaaaggata
    56821 cacttttcgc gataatatac ccactatttt aaagtcatta atgagtgcgg taatgatggg
    56881 cgcagcaata tatttattgg atatatctct tatgccggca atattcggaa cggcaattat
    56941 tcaattgggt aagataaagc aatttctatg gatacttatt gagattggta taggtgccgg
    57001 aatatatatg ctccttacat atctgatgaa agccgatcag gcacgcgaac tgatagatat
    57061 ggtaagagca aaatttaaga gcataattgg taataattag caattacgaa aaaatattcc
    57121 ttgtatgtat ttttaagatg atatataata ttggtatatt caaaaaagat tgaaaaagga
    57181 gcatattatg gataaaaaga aagcattgga agcgtcactg gccgggattg aaaaagcata
    57241 cggtaaggga gctgttatga gactgggtca aaatacggcg atgacagtgg aatctatacc
    57301 gacaggtgca atatccttgg atatagcatt gggtatcggc ggtgtgccaa gaggcagaat
    57361 aattgaaata tacggaccgg aatcttccgg taaaaccacc gttgctctgc atattattgc
    57421 agaggcacaa aagggaggcg gcgaagctgc atttattgat gcggaacatg ctttggatcc
    57481 catatatgca aaaaaactgg gagttaacat agacgaactt atagtcagcc aaccggatac
    57541 aggcgagcag gctttggaga tatgtgaagc tctggtaaga agcggagcat tggatgtaat
    57601 tgtaatagac tcggtagctg ctctggttcc taaagccgag atagacggcg aaatgggaga
    57661 cgctcatgta ggactgcagg ccagattgat gagtcaggcg ttaagaaaac tcacagctgt
    57721 cgttgcaaaa tccaaaacca caaccatatt tatcaaccaa ttgcgagaaa aagtaggcat
    57781 aatgttcggt aatcccgaag ttactccggg cggaagagca ttgaagtttt attccagcgt
    57841 cagaatagag gtcagaaaag ctgacaacat aaaattgggt gataacatca tcggaagtaa
    57901 aacaagggcc aaaattgcta aaaacaaaat tgctcctcct ttcaagacag cggagtttga
    57961 tattatttac ggacacggaa tatccaaaga aggatgctta attgatgtcg gagtggactg
    58021 cggagtagtg gataaagccg gatcatggta ttcatataaa gatcagcgga taggtcaggg
    58081 acgggagaat gccaaagact accttgtgca gaaccccaag atagccgaag agattgagaa
    58141 gctgataagg gcagcatttg aagcgcataa aacgcccgaa caaccgacag aagctgcaga
    58201 tgaatgatga attgagcatc gtaaaaagag aagtattaaa aaaagactat atccttactt
    58261 tatccgataa tacacaactt tttattgatg aagaaacata tttcaaatat tgtatctacg
    58321 acaaagaaac attatcaaat gcttttattg aggatattaa agataaaacc gaagcgatgc
    58381 aatgctataa aaaggcggtt gcttatcttt taaacggaaa aaaaacggaa aatcgtatga
    58441 ggttatacct tgagaacaaa ggattcgggc ctaaagcggt agacagttgt attcaccgtc
    58501 tgattgagga aggaaagata gatgatgttg cttttatgga caagttcata aaagccaatc
    58561 tgaagaagga cacaaagaga gaaaaactta tagcaaaact gatataccac ggaatagatg
    58621 agcaactggc agtacaagag gtggataaag cggtaggata tgaagaagat acttattaat
    58681 tcactgggat gtcccaaaaa tctggtggac agcgagataa tggcaaagtc actgaaagat
    58741 aaaggttttg ttcttaccga caatatcgaa gatgcgaaca ttgcaataat caatacatgc
    58801 tcttttattc aacccgccgt ggaagaagca atcagcgtaa tattggaagc cgcggaatac
    58861 aaaaaacggg ggattctgga aaaacttata gtcacaggtt gtctggtgga aagatataag
    58921 gaggagataa taacttcaat acccgaagtg gatgtatgtg tgggaataga cggctataag
    58981 gacatagcat ctgttgttaa taatacggat gaagttttga tttgcggaaa taaaaccgat
    59041 gtaaccttta tgaataaaga acgaatgctg tcggaaaata ccgcatcgac atatgtgaaa
    59101 attgccgaag gttgtgataa cagatgtact tactgcacca ttccgtccat ccggggaaga
    59161 tacagaagca gaagtatcga ggatatatat gctgaagcgg agaagttgtg ctctctgcac
    59221 ggtataaaag aaattacact ggtggctcag gatactaccg catacggtac ggatatttac
    59281 gggaaaccgt ctttggattt gttgcttaaa aagttggaaa cagtcaaatc tttacagtgg
    59341 ataagactgc tttactgcta tcccgaactt atcactgacg accttattaa ttccgttaag
    59401 aacagcaaaa agatattgca ctatctggat atacccttgc agcattcttc cgacagaata
    59461 ttaaaactga tgggacgtaa gggaacatgt gccatgtata cggaattaat agataaactt
    59521 cgatttcata tacccgatat agtgttaagg accactttta tagtggggtt tccgcaggag
    59581 acggaagaag atttcgagaa cctgtgcgag tttataaagg catcccgttt tgacagagcg
    59641 ggtttctttg agtattgcag ggaagaaggc acaccggcat acagactgaa aggtcagata
    59701 ccaaagaagg taaagattca aaggcgcaaa caggcggaac aaattcaaca ggatatatta
    59761 atcagcaaaa atgatgaaag gataggcaaa acatatagta taattataca aggcatttcc
    59821 gatgacggat tgttctatgt gggcagatct tatgcagagg catatgaaat agatcccgtc
    59881 atatatgttg cagccaaaga agagttaact gtcggagata taacagatgc cgtgattgtt
    59941 gccaaacatg aagattcact gattgcggag gttgtgcaat gaatatagcg aataaactta
    60001 ctctgataag aataatattg ataccttttt tcatcatatt tgccgttccc acaccggaat
    60061 ggttgagtga accggttttt tcttttcatc agcagtacgg tacattaatt gcgttggtaa
    60121 tttttgtaat tgcttcctta accgaccttt ttgacggata tctggcgagg aaatacaaac
    60181 tgataacaaa tatgggtaaa tttttagatc ccattgccga taagatactt atcacaagtg
    60241 cattggttgt actggtggaa agaggacaga tagcggcata taccgttgta ataattattg
    60301 ccagagactt gattgtagac ggattgagac tggtggtaag tgaaaagggt gaagtgattg
    60361 ccgctaatgt attcggcaag attaaaacca atcttcagat aatcagtgta tgcttgtatc
    60421 ttctcgcttt ttatatcaat ctttggtata tacctaagat tgtttacgca ttggctttga
    60481 tagctaccgt atattcggga gttatatatg ttaaggagtc aattaagttc attaaagatt
    60541 gatttttcat ttattttcgg agaaaagtta aggaaaaata atgtatatct ctttacaaag
    60601 tatgtgggat agaatatact gatgtaaatt tgttatcagg taataaaatg ttttgaaggg
    60661 aaaaggatga caaaggcgac actgaaaaaa ctgaagaaac ttgaagattt gcagatattg
    60721 ctttcggaaa gtccgtatat gaacgataag cagcttgccg aagctttggg agtatcggta
    60781 ccgtgtataa ggaattacag aaatgaactg ggaataaagc aggttcgtga aagaataaaa
    60841 gacacggtaa gatcgcttgc acctgaggac atagtgggaa ctataacaaa tctgaaactt
    60901 aatgaaggag cttctgcgga atttgttaca acggcggata tggttttcaa gaaaaccaat
    60961 atagtcagag gtcagtacct gtattccttt gcggaatcgg tagctatttc cgttatagat
    61021 gcagaagctt ctctggtagg ggtagcaaat attaaataca aggaaccggt atatgccaat
    61081 gaaaaactta ccgcacaggc caaagtaaca caaataaggc tgaataacta ttttgtatgg
    61141 gttttcgttt acagagagga tgtagagatt ttccgaagca aatttatttt ggtttcggta
    61201 tgatgtgagg atttatgaat atacttgtag atgctatggg cggtgacaat gcgccggaag
    61261 caatagttat aggatgttta aatgcaataa agacggaaga aggttttgat ataaccttat
    61321 tcggtgacag caaaattatt gccgatatac tttgcaacta taactatgat aaatcgagaa
    61381 taaaagtaga gcattgttcg gaagtaatac tttttaaaga tacacccaca gttgcggtta
    61441 agaacaaaaa ggactcttcc ttggtcagag cacttactgc gatgagggaa gggaacggag
    61501 atgcggtaat atccgccggt aataccggtg cactgcttac aggctctctt ctgataacag
    61561 gcaggatgca aaaagtaata cgcccggctt tgggagcatt gattccttcc ggggaaaagt
    61621 ttgctctatt gatagatgcc ggactgaact ccaatttgcg tcctgaaagt tatgtgcagt
    61681 tcgcaaaatt cggttccgaa tacatgaaga tagttacaaa atgcgataat cctttaatag
    61741 ggctgttgaa tataggaacg gaagacacaa aaggaagcgc ggaagtaaaa gaggccaacc
    61801 tactcttaaa gcagtcggga ttgaactatt acggcagtat agaagggaac agtatcgtag
    61861 aaggtgttgt ggatgttgtt gtaaccgacg gttttacggg aaatgtatct cttaaagttc
    61921 tggagggtac cgcatcgtat attttcagta aaatgaagtc tgtattcagt atggatgttt
    61981 tccacaaact tgccgcatca gtgataagac ctgacatgaa gaaatttaaa gataatttga
    62041 atcccgacat aatgggcgga gcaccgatat taggagtaaa cggtttaata ctgaaatcac
    62101 acggaagttc caatgccaaa acaataatgc atgtggtgct taaagcggtg aagctggcaa
    62161 aaaatgatat agtggggaat ataaggagag ctgtatgtta aacgctattt gcgttttaat
    62221 ataaaaaagg ttaatattaa cgatggaata atattatgaa tattaaaaac acgtccatcg
    62281 tgagacggga ggataaaatg atttttgaga aggtaaaagc gattatcgta gaacaattgg
    62341 gtgtggaggc agacgacgtg acaatggaat cttcatttgt ggaagatctg ggagccgatt
    62401 cattggacat tgtggaactt atcatgtcaa tggaagatca gtttaagatt accatacccg
    62461 ataccgaagc ggagaacatt accacagtag gtagtgccgt aaagtacata gaaagcaaca
    62521 ggtaggagaa tcaaggctat ttcaatagcc ttattttctt aagatttaaa aatctgaaga
    62581 aaatgaggca gttttgcaac cggatattct ccaattacaa gaggtatgca tatcggatac
    62641 aggtaaaatg gaaaaagtga tagataaatc aatagcatcg gtagagaaag ctataggtta
    62701 ttcttttaag gataaatcat tgctgtctac cgccttaaaa cacagctcat acgacaaaac
    62761 ggaaaattac gaaaaactgg agtttttggg tgattcggtt ctttctctgg ttatatcgga
    62821 ttttcttttc agaactcaca actatttgca ggaaggtgag atgacggtaa caagagctta
    62881 tgcggtgtgt gaggaatcat tatccaaagt aagcaaacga ttgggaatag acaaatatat
    62941 acttatcggt tcgtcagccg taagttcgtc cgtcaataca aacaagtcgg ttatagccga
    63001 tgcattcgag tcgctgatag gtgcaatgta tttggattcg ggatttaaat atgtccgacc
    63061 ttttattatt tccaatttga acgagtttat tgtagaatat atcaggtcgg gtgataataa
    63121 tgatcacaaa acgaaattac agcattttgt acagtccaga tatcaggaag agcccaaata
    63181 tctggttaaa ggagaaaaag gaccgcctca tgacaaaaca ttccttgttg aagttataat
    63241 tacagggaaa ccgtacggaa aaggtatagg aaaaacaaaa aaggaagcac agcaggctgc
    63301 tgcaaaagat gctttggaga atattgagaa aaaaggttta gataattatg attcttaaaa
    63361 aagtggaatt gcagggattc aaatcctttg caacaagaac agtgttggaa tttaacaaag
    63421 gtataaccgc aatagtaggt cccaacggga gcggaaagag taatattgcc gatgcgataa
    63481 ggtgggtttt aggttcgcaa agttccaagc agttaagagg cggtaatatg agtgacatga
    63541 tattctcagg tactcaatac agaagaccgg tcggttttgc cgaagtttct ctatactttg
    63601 ataacagtga caagcatctg gcggtggatt tcgaggatgt caagattacc agaagactgt
    63661 accgttccgg tgaatcggat tattccgtta acgataagag ctgtcgtctg aaagatattc
    63721 agatgatgtt cttcgatacc ggggtaggta aagaaggata ctctatcata gggcagaatg
    63781 aaataaaaga tatcgttgac agcaacagcc tggaaagaag aggtatcttt gaggaagctg
    63841 ccggtattat gaaatacaaa cagaagaaac tggaagcatt acgtaagctt tcaaacaccg
    63901 aagaaaacct tgtaagaata accgatatca taagtgaaat agaaaaccag ttggagccgt
    63961 taagattaca ggccgagaaa gcaaaggaat acttaagagt atacgagata ctgaaagtta
    64021 atgaagtggg actgtatctg aataatctgc agatgtatca gaaacgcatt cagaaatacg
    64081 aacaggatat acaggttttg tcggaagaca ttttgaccag agagtcggaa ttggagaata
    64141 taaagcagga aaacagggac agtattaata tcatcaaccg gtatgaggaa caggaacaaa
    64201 acctcaaaga tgaattgaac acctttttaa gagacttgga gtcacaaaga catgaaataa
    64261 tattatgcaa cgagcgattg tccaatgccg acagtgaact tataaaaaac ggcaaagaaa
    64321 tagaagaata tcaattaaga gagaatgaac tgtccaaaga ttaccttaca aaagaagaca
    64381 gaaggaagta tctggaaaat caatacacaa gctataaaga gaaattggat ttattgaccg
    64441 cacaggcgga aagcattacg gcatcgctga gcggaagcga gaaaatactg gaaggtgtga
    64501 aagcatcttt aatgagacat atagaagaga cttccgacag taaactcgta ttaaataatg
    64561 tcatcaatga aaagaatgct ctgaagactc agctggacat gatggataac gatataaaca
    64621 aaaaggtgtt ggagaatgat tcaatactat tagcaaagca ggacaatcag agcaaaaaca
    64681 ttgccgccaa gaagcagcaa gagcagctgg cctcaagaag agataaaagc aagaccgagt
    64741 acgcattact gcaaaaacgt atggagcagg cggaatcggc agagcgtaat cttaaaacca
    64801 atatagagat actgcaaagc aactacaaac ttatatacga catggaaaag cagatgcacg
    64861 gttataagca ggatgtaaaa gcggtgctgg aaaataaatc tcttaacgga atagtggatg
    64921 tggttgccaa acttatgggt accgaagaga tatataacac ggccattgaa gcggcgttag
    64981 gcggaaatat tcagaatgtc gtagtggagg atgaagaggc ggcaaaggcg gctattgctt
    65041 tcttaaaaga aaacaagctg ggacgagtga cattcctgcc ggtaagttcg gtgcgcccca
    65101 atcagtttaa caaagatgaa gaaagagaaa tactgtcata taaaggtgtt attgatgttg
    65161 catataaatt attagaatat gattcgaagt atgaaaatat agcaaagaat cttttaggca
    65221 gaatattagt tgtagacact tacgacaatg ccgtacttat agcaagaaaa acacagagca
    65281 ggtataaact tgtaacgctt accggagagt ttttcaatac cggcggctcc ataaccggcg
    65341 gtactcagaa tgtaggtaag gtggggctgc tgggaagaca caagcaactg gatcgtctgg
    65401 agagtgaaat agctgcacac aaagaaaaac tggaacagtc ggttacacag agtcgttcca
    65461 tgtctaaaca ggcatcggaa ttggcttcgg atatagacaa gacggagcaa tgtattattg
    65521 aaggcaatat gcttacagcc aagctggaat cggcaatatc cgcacttgat gaagcatata
    65581 aaagtaatac ggatgcactg aatgccacaa agaacagaaa gaaagagtta acggataaat
    65641 atcacgggta tgacgataag atagcgatac tcaataatca gatagatgcg ttaagtgagc
    65701 agattgaaaa ggataaactt aagattaacg agtatgaaga aggacataaa gaacagtcgg
    65761 caaagagaga taatatttat ctggatatat ccgatatgaa ggtttcggtt aattccatag
    65821 aagaaagcct caagaacaac gatgaatcgc taataaggat aggttttgaa aaacaggctc
    65881 ttaccgttaa gcgtaatgaa ctgataaaga gaaacgagca acttaaagaa ataaaagccg
    65941 accttcagga taagatacag acaaagtcca aaaacataag taatcccgag aaagttgaga
    66001 aagaaaagaa taaggctata caggaattgc agaacaagag aaagaagtta tcccaagaga
    66061 acgagaaata cattcagaat attaccgatg tcaacaggga tataatgctg ctgtccaata
    66121 agatttcttc cattgaagtg aataaggcga aaaccgatgc cgagatgacg gcaatgaaag
    66181 aacgtatgtg ggacgaatac aatttaactc acaacgaggc attacaaaga cacggcgaga
    66241 tagaaaacat aaccgaggcc agaaaagaaa tagccgaggc aaggaacatt ataaagcagt
    66301 tgggtccggt aaatattcat tccattgaag attatgccaa aaccaatgaa cgatacagat
    66361 tctttacaac acaaaaagag gatatagaga agtccaaaga agacctgtat aaaacggtta
    66421 atgaaatgaa tacggtaatg aggaaaatct tcaaagagaa atttgaaata atcaatgata
    66481 atttcaacga gatttttgcc agactgtttg agggaggtca ggcaaaactg gagcttaccg
    66541 accaatcgga cattctggaa tgcggtatag atataatcgc acaaccgccc ggaaagaagt
    66601 tgcagaacat gatgctgctg tcgggaggag aaaaggcttt caccgcaata gctttggtat
    66661 ttgccatgct gaagctcaat ccctctccgt tctgtgtatt cgacgaaata gatacatcat
    66721 tggacgacaa taacgtactc aagtactgca aatatctcaa cgagcataag agtaatacac
    66781 aatttgttct tataacacac agaaagggga ccatggagaa tgccgatacg atatacggtg
    66841 tcactatgca ggaacacggt ataaccaaag tagtatccat gaagattacc gaataatatg
    66901 agtttttttc gacgattaaa agaaggtctt tccgcaacaa aagaaaaact cactaaacag
    66961 attgataaac ttctggcatc ctttcgcaaa attgatgaag agttattcga acaactggaa
    67021 gaagtgttga ttgagagtga tattgcaata gataccgtaa tgcagattac cgagcagctg
    67081 aaacaggaag ttaagattaa caatatcagc gatcctaaac agattaggga tttgttgaaa
    67141 aagaaactta tggatatgat aacaaaaaac agcaatacga ctctcaatgc attgaaggat
    67201 gattataaag acaaattgaa catcattctt gtagtgggag taaacggaac gggtaaaact
    67261 acttccatag gcaaacttgc gggaatgttg accgggcaca acaaaaaagt tctgcttgca
    67321 gcggccgata ctttccgggc ggcggcgtct gaacagttaa tcgagtgggc aaaaagaacc
    67381 ggtgtggaca tagtggcaca taaaagcggt gctgacccag gtgccgttgt atatgatgca
    67441 attcatgcat cggtaaaaag gggagcggat gtcttaatct gcgatactgc gggaagattg
    67501 cacaccaaga gcaatctgat ggaagaactt aaaaagataa gcagggtaat aggcaaagag
    67561 gctcccgatg cgatagtgga aacactattg gtaattgatg catcggcagg gcaaaattca
    67621 ttggtacagg caagggtatt caacgaatac gtaaagatag acggaataat actgacaaaa
    67681 ctggacggta cggcaaaggg cggaatggta atatccatct gcaatgaact ggatattccg
    67741 gtgaagtata tcggtatagg tgaaaaagcg gacgatttgc gaccgtttga cggaaaaagt
    67801 tttatagaag cattgtttga caattaatat gacatatgct tataatatac tgaaaagcaa
    67861 ggatccgaag agtaaatatg cttatccttc aagagagtat gtggtcggtg caaacataca
    67921 ggcaggcatg ttgaaaatca tcggggagcc ggttacccga atacaagcag taaggtaacc
    67981 cggaacagac cgttatcgta attaagcggc ttttataagc aacttgggtg gtaccgcgaa
    68041 tactctttcg tcccttgaga tgagagagtt tttttatagg cattaagccg gaaagaggaa
    68101 gttatgtata aaaaagtatc aactgatttg aatttcgtaa agcgggaatt ggagactttg
    68161 gagttctgga agcaaaacag agtcttcgaa aaaagtatag agaacagaga gggatgtgag
    68221 catttcacat tctatgacgg tccgccgact gctaacggca agccgcatat aggtcatata
    68281 cttaccagag tcataaaaga cattattccc agatataaga ccatgaaggg atataaagtg
    68341 ttaagaaaag ccggatggga tactcacggg cttcctgtgg agctggaagt ggagaaaatg
    68401 ttaggcctgg acggaaaaga cgatatagag cgttacggaa ttgaaccttt tattaaaaag
    68461 tgcaaagaaa gcgtctggaa atataaaggc gaatgggaaa agatgagtga tcgtgtgggt
    68521 ttctgggcgg atatggaaaa cccatatatt acctatgaca acaactatat agaatcggaa
    68581 tggtgggctt tgaagaaaat atgggatatg ggtctgctgt acaaaggaca taaggtagtt
    68641 ccttattgtc ccagatgcgg aaccagcctt tcttcacatg aagtggctca gggatacaaa
    68701 gacataaccg acaagaccat atatgtcaag tttaaggtta aggatactgc cgatacatat
    68761 tttatggcct ggacaaccac tccgtggaca ttaccgtcaa acgtggcact tacggtaggt
    68821 gcaaagtttg attatgcaaa ggtgaagtat aacaatgagt atataatcgt tgccaagcaa
    68881 ctggtccctt cggtatttga aggatcggat tacgaaatag tgtcgacggt aaagggaaaa
    68941 gagctggaat acatgaagta tgagccctta ttcgattttg tcgatgaaga tgcaaagaaa
    69001 gaaggctttt atgtatgttt aggcgatttc gtcacattga cagacggtac cggtattgta
    69061 cataccgctc ccgcattcgg tgaggatgac tcgataatag ggaagaaata taaccttccg
    69121 tttttgcaga tggtggacga agccggacac atggtagagc agacgggatt tataaaaggt
    69181 ttgtttgtta aagatgccga taaaattata atcggggatt tagataagcg cggtcttctg
    69241 ttcaagtcct cggaaatcgt acactcctat cctttctgct ggagatgcga caccccttta
    69301 atttattatg cccgttccac ctggtttata aaaatgacgg caatacggga cagtcttctt
    69361 aagaataatg acaaaataaa ctggatgccc gccacaataa aagacggacg tttcgggaat
    69421 ttcttacgtg cggtagtgga ttggggaata tccagggaac gttactgggg aactcctttg
    69481 cccatttggg aatgcgaatg cggtcacaga gaggctatag gaagtattgc ggagttgaag
    69541 gaaaaagcag tcgattgccc aaaagatatc gaactgcata aaccctatat cgacaatgtg
    69601 catcttaaat gtccgaaatg ttccgcgatg atgaagagag ttgcggaagt aatagactgc
    69661 tggtttgaca gcggttccat gccttttgct cagcatcact atccgtttga gaataagcaa
    69721 gtatttgagc agggctttcc ggcagatttc atatccgaag cattggatca gaccagaggt
    69781 tggttttata cattacatgc catttcctgt ctgctttttg acagcccggc atataaaaac
    69841 tgcatagttt taggtcttgt tcaggataaa gaaggtcaaa agatgagtaa gcataaaggt
    69901 aatgtagtgg atccttggga tgtgctggag aatcagggtg cagatgcggt gcgttggtat
    69961 ttctatacgg taagcgctcc gtggctgccc agtcgtttcc acgataaagc cgttaccgaa
    70021 gggcagagaa aattcatggg tacattgtgg aatacctatg ctttctatgt actttatgcc
    70081 gaaatagata aatttaatcc actgaaatat cggatgaaga aagacacgct ctgtttaatg
    70141 gacagatgga tactgtccaa acttaacact ttagtaaaga ataccgataa aaatctggtg
    70201 aactacagaa tattcgaagc ttccagagag attcaggagt ttaccgatca attgagtaac
    70261 tggtatgtca gaagatcgcg cgaaagatat tggggaagcg aggagactca agacaagata
    70321 aacgcttatc tgacattgta caccgctttg gttactttag ctaaactggc ggcaccgttt
    70381 acacccttta taaccgagca gatatacgga aaccttgttg ttaacctgga taaaagcgct
    70441 cctataagcg ttcatctgac cgattatccc gtatgcgatg aatcattaat cgataaggaa
    70501 atggagaatc agatggaaga gttgcttaaa gtaattgttg ttgccagaag cttaaggaat
    70561 atttccggta taaagaacag acagcctctt cgtaaccttt acatatcatc ggaaaagaaa
    70621 cttaatcaag agtatatcga gcttctgaaa gatgagttaa atatcaagga tataacttat
    70681 gtgaccgatg cgtccatgtt catagattac agcctcaagc cgcagttgag aacattgggg
    70741 aagaaatacg gcagattatt gcctgcgata tccgaacatc ttaaaagtgc cgacggtgct
    70801 catgttatgg gactgtttga aaaagggttg aattatgagt ttacggtaga gggaaatgca
    70861 gtagtactgg aaaaagacga tgtgctcagc gagactaagg agaaagacgg tcttatatcc
    70921 caatcggata acggaataac cgtggttctg gatatcaatc tggacgagca actgattgaa
    70981 gagggatacg taagggaaat agtcagtaaa atacagacca tgagaaaaga tgcaggttat
    71041 gatgttaccg acaagataac cgtatacatc gaaagcagtg acatgctgga gaaactggtt
    71101 gtgaaaaatt tcccctcgat tatgaaggat gtacttgcaa cgggcattgc caaagcagcc
    71161 gatgattcat tctattgtgc gacttgggac ataaacggag tacaggcgga tattaagata
    71221 aagagagttt gatgcatata atcaaatccg atgctcattt atcttaccgt tattgaataa
    71281 attggtatat tattgtaata taaagtttgc ttgattatga gaggtgacaa aatgaaacaa
    71341 ttgaaagtag ctatcatagg acagggaaga agcggatact ccatccacgg aacatattta
    71401 attgatgata ccgaaaggtt taaagtgatt gccgttatcg attttatcga aaagagaagg
    71461 cttcttgcaa aagaacggta cgggagccat gtggtaactt cggataacta taaagatttg
    71521 ttcggaatgg atatagattt ggttataaac tcatcttaca gccatttgca ttacagtatc
    71581 acaaaggatc ttttaatgca cgggtttaac gtacttacgg aaaagccctt ctgccgcact
    71641 gcagacgagg tacaggatct gattgatacc gcaaaaaagc aaaaacgtat gcttgcggta
    71701 ttccagaatt ccagatttac cgcttacttt atgaaaatta aggaaatact ggattctaaa
    71761 gtgcttggag atataataca gatcagtatc gacttttccg gatatgcaag aagatgggat
    71821 tggcagacat tgcagtcata taatgcgggt aatttataca atacgggacc gcatcccgtg
    71881 gatcaggctc tgcaactcta cggagaaggt atgccggaag tcaaagcttt catgagaaga
    71941 gtaaatactt tcggagatgc ggaagattat gtgaaactca tactgtacgg agagaacaag
    72001 cctttaatag atgtggaagt aacctcctgt cagggatata aaccttatgt gtataatatt
    72061 cagggctcta aaggagcgct tacgggtact atgacacaaa taaactggaa atacttcaaa
    72121 accgaggaac ttcccgagca gaaactgtta ttgtcctttg acaaggaagg aaccgatata
    72181 ccggcttatt gcagtgagaa tataaaatgg tatgaagagc aatggaccag tgaaggcgag
    72241 aaatacggta ctttcggagc gtctaccaga agatattacg atatgttata taatcatatg
    72301 gttaacggag gcgagcttac agtaacagcc gagcaggtta aaaagcagat agctgttatt
    72361 gaagaagcac acagacagaa tccgttgagc aaaaagtttt gaggagaaac aatgaaggaa
    72421 acagaaaata aagaaactat taaatccgac gaagtccaac aggtttccga tacagacacc
    72481 gttgaacata ttgcagaaga ggatataaat accgaagagg ataatataac ggaagaagtc
    72541 agtgagaaaa ggaatgtaaa gaagtccaag ttctcgcaac acttgaaaaa attcggattt
    72601 tattacgggt tgggattact gattgtcata ttggcggtcg tccttatttt cgacggtaac
    72661 cgaagattaa ggggcgatat accgtatgat gataatatct atattgtggg agataattcc
    72721 atttccatga taagatataa ccaggaaccc gtaaagatta catcacaact gttcaggaac
    72781 tacgaagatc agaaactggg actggaagct cataacggaa tattaatgag taaagacggg
    72841 aatagtatca tgtatgtgga tgatctttac cttttaagca atgaggattt atcattgact
    72901 aaactggtcg gtaccctatg gatgtacaac ggtgtaaaga atataaaaat tgcggatgat
    72961 gcggacttct attacgttgt taacgatgat ttcaatatag ttttatacaa gacctatcgg
    73021 ctggcggaag atgagtcaag acttatctcc aacctgtata agtttgacct tgcaacggga
    73081 caatcggtta tcttaaggga agatatcgat actgacctgt attcggtaag taatgacggc
    73141 aaagtatgcg tattcttaga cgaaaaacag gagagttttg ttgagaacgg tgagccgaca
    73201 tattcactgt ttgtatacaa agacgggcag accgaaaagt tggcggacag cgtttatgat
    73261 ttgggtgctt ccgtttatac gggtactgcc gaagtaaatt atcctatcat aagtaaaaac
    73321 ggcgataaaa tagtttatgc tgtagtggat tacatataca tggtggaaga tgagcaagag
    73381 gagcaacccg aagatgtaga agaaacacaa acggaagaag aggagaagaa agtcgtctat
    73441 cctacattca acatgtatct gtatcatgac ggagtttcca aactgattgc aaaaagttgt
    73501 gcacagcttt taatctccga agatttttct caaatgattt atatcgatgc ggtagtcgaa
    73561 catttagcag catatgatga aataggattt tgcggaacat ataatcacta tgatgtggag
    73621 acaggaaaat ccaccaaagt aatggataac gtgttcggat ttgtcgaaag aaacgtattg
    73681 ggttttgtgg acaatgactt tatagacgca aacttctatt ttaaacaata cgatattgta
    73741 atgaataccg ctcatctgta cacaataaga aacggaaagg aaatactgat tgacgattat
    73801 gcatcggtag gggaggatga agatcttaat ataaaacttg tattcttttc acccgactat
    73861 aaagatgtat atgtttttaa ggaatacgta agtaagacaa gcagcttgct tcacagattc
    73921 accattaaat ccaacggatc cataagtgac tacatgtgtg aggaaatgca ggttataagc
    73981 gctattgcac tgtcaaaaga cggagactat cttgtataca atgcggacgg taagctcatt
    74041 attatatccg acaaaaatgt taaaaaaacc atagaggtaa acggagtatc atcattcggt
    74101 attacggaag acggaaagta tgtgtactat tataaagaaa ccgagatagg tttaggtgcc
    74161 gtttattact atgacctgta taaaagcgaa aagccggtca agtttctgaa caatgttaat
    74221 gaggtatgga actataaaga caacaaggtt atgctcagga taaacaccga ctataataca
    74281 atgacgggtg atctttatct gtcggatttt gaacagttcg agtacatatc tcccgacgtt
    74341 tatgcacaga taacaactaa agtaactgaa aggtagataa aaattactgc tttaaaaata
    74401 ataccgctgg gcggactgaa tgaaataggg aaaaatctca ctgtctttga gtataggaat
    74461 gatattctta tactggattg cggtataaag tttccgcagg aagatatgcc gggagtggat
    74521 atagtcaaac cggatatgtc ttatcttgta gaaaataagg ataaaatcaa gggagttgtc
    74581 attactcacg cacatgagga ccatatagga gccgtctgtt atcttctcaa ggaaatcaat
    74641 acccccgtct acggtgcaaa actcacgtta ggattattgg aatccaaatt gaatgagatg
    74701 aatctgctga caactacatc gctcaatatt attgacggag attcaataat atccataggt
    74761 tgttttaaac tggaattcat tcccgtaaat cacagtatac cggacacata tgcagtggtc
    74821 ataaacactc cggtcggaat aattcttcat acctcggatt ttaagattga ctataacccg
    74881 gtaaacgaaa aagtgaccga tctgaacagg ttctcggaat tgggaaacag gcatgtgctt
    74941 ttgatgatgg cggacagtac aaatgccaca aaagaaggac acagtatgag cgagagggcg
    75001 gtaggcgatt cattggataa tctgttcaga aaagcctctt cacgtattat tgttgcgtct
    75061 ttcgcttcca acatacacag aatacagcag atactgaatc ttgcggaaaa atacaaaaag
    75121 aaagtatgta tattcggatt cagcatgcgt aacaatataa gaaaatctat agagctggga
    75181 tatatacagg ttaaaagagg cattatcata gaagattttg aaataaagaa ctataaacac
    75241 aaagacatgg tctttatagt gacaggctcg cagggagaga ctaactcggc attatcgaag
    75301 attgctttta aggagcatca gaagattagt gtggagaaaa atgacctggt tatcatctcc
    75361 gcttccgtaa taccgggaaa tgaaaaatat ttatatcgtg ttataaatga attattcaaa
    75421 agcggcgcaa atgttatata tcaggctctc aatgaagtac atgcttcagg acacgcttac
    75481 agacaggaat tacggctttt gcttacactt gtacgtccta agtactatat gccggtacac
    75541 ggtgaattta tgcatatgga gagtaatgcc ttattagcca tggaaatggg aattaaagag
    75601 aagaatatat ttcttctgga aaacggtgat gtcatgaagg tctatcccaa tagaatttca
    75661 gtggatgata aagataaaat aaaggtaggg ttatcttttg ttgacggaca cggggtaggc
    75721 gaattgggtg atgacgttct gaaagaaaga gaaattcttg ccggcggggg aatactcaat
    75781 atttccatag tcctaaacaa atcggataga aagtcagcgg ttgaacctgt tataatccct
    75841 gtaggattcg tatatgagag tgaatcggat gatataataa gaaagataag agaaatgctt
    75901 gatgaagata tcgggaagta tacgaaaact tcaatgagca ttaatgaatt gcaaaactcc
    75961 gtaaagaaac gtttgagaaa atttctttat caggaaacaa aaagaaatcc ggtagtactg
    76021 ccggttatta tggaagtata ggtggaaaaa tgaaagcgta tgatatttta aaagaaagag
    76081 gctttatagc ccaggcgaca gatgaagaag ctatcaaaaa tcttcttaat aatgaaaaaa
    76141 ttgttttcta cataggcttt gaccctacgg cggacagtct gcatataggt cattttcttc
    76201 agcttattgt catgagccat ttgcagaagg cggggcataa gcccattgca ctgttgggag
    76261 gcggcaccgc tatgataggc gatcctaccg gcaaaaccga tatgagaaga ataatgacaa
    76321 gagaagaaat tgactacaac gcaagctgtt tcaaaaagca gatgtcgaga tttgtggatt
    76381 ttaccgatga cagagctatt atggtagata actacgaatg gctgtccaaa ttggagtata
    76441 taccgttctt aagagaaata ggaatgcatt tctccgttaa cagaatgctt acggcggagt
    76501 gttttaaatc ccggttggaa aaaggattat cctttataga attcaactat atgctcatgc
    76561 agagttacga ttttctgtta ctgagaagga aatacggatg tatcctgcaa ctgggcggag
    76621 atgaccaatg gagcaacatc atatccggag tggatctgat aagaagaaaa gaacttggag
    76681 aagcatacgg aatgacattc aatcttctta ccacaaacga aggaaagaaa atgggtaaga
    76741 cggaaaaagg agctatctgg ctggatgaga ataagacatc tccgtttgat ttctatcagt
    76801 actggagaaa tatagatgat gcggaagtta ttgagtgtct caaattgctt acctttttac
    76861 ctatggaaga gattaaaaaa ttaagcaaac tgaaagatgc ggaaattaat catgccaagg
    76921 aagttctggc ttatgaagtt accaaaatag ttcacggcga gaaaaaagct gatgatgcta
    76981 agatagcttc acgagcatta ttctccaatg taggagagat aggcaatatg ccttccaagg
    77041 agttgacaaa gaataatctt gaagaagggt atactattct tgatgctctg acagatgccg
    77101 gatttataaa gtccaacagt gaaggcagac gtctgataca ggaaaaatgc gtatccgtta
    77161 atgatattgt tgtggaggat tttaaattat gtataaacag cgacatgttt accgacggtg
    77221 aaatgctttt aagaagaggt aagaagaagt atttcaggtt tatacttaaa taattaaatt
    77281 gatgttgcaa attttattaa tatttgattt aatagaatac agtattttta aacaagcgga
    77341 gggtttaaat tatgatttat gatgatattt cggcggcatt acaaaaaggt agaaggaaag
    77401 atgtagaagc attgattcag caggctatag atgacaaatt accgccaaaa gatatactta
    77461 acaaaggact tcttgcagga atggatattg tcggtgagaa gttcaagaac aacgaattat
    77521 acgttcccga tgttcttgta gctgccagag caatgaatgc cggaactgca ttacttaagc
    77581 cccttttggc tgaagcggga gtggaatcaa taggtaaagt ggtgataggg accgtattgg
    77641 gcgatcttca tgatataggc aaaaaccttg taaaaatgat gatggaaggt aaaggctttg
    77701 aggttataga tttgggcgta aacgtttccg cagaacagtt tgtacaaagt gctattgagc
    77761 acgatgccga gattatcgcc tgttccgctc ttttaacaac aacaatggga gaaatggcca
    77821 atgttgtaaa agtagcaacg gaaaaaggca taagagataa agttacaata atgataggcg
    77881 gagcaccggt taatgaagcg tttaagatct cggtaggtgc cgatatctat acacccgatg
    77941 cggcaagtgc tgcggaagaa gctgccaagg tagtattgaa ttaacataag tttatatttt
    78001 taacattaac gagagttgcc acatttagca gtggcaactt ttaaatttat tattttatct
    78061 ttttgtcttt aacaacttta acagaggata aatttaatca atcaaacata ggtttaacgg
    78121 aaaactcatt aagatattgt gacttatctt tcaaacagac gatattatag atatcacaag
    78181 ccttgttttc aaactcaatg agagttttca ggtccgagtc gaaatcatca tatgactttt
    78241 ttaaagatgt tattacaggt agagaggatt ttgaggaaat cagggataag agtgatttgt
    78301 ttctgcatgc aagcactctg atataacctg gcctcttata cttatacgta atacccttat
    78361 tcatacctaa tagtatatga ataagcagtc gttgtattct ggttgcggga tatcgtttat
    78421 taacacaggt atttataaaa tcgttaacgg aactgcagga aaacacattg ttttttagca
    78481 gattctcaat accttcgttt accaaaggat actttttcag ttcatcatta ctttttgata
    78541 taaccgagta aaaaatgagg ttctcaaata gatacatgga gccgaaatgc ttaacctctg
    78601 agtaatcttc caatattttt aatgttgagg ggggaagatt gtctgccaaa tctttattgt
    78661 taataacttt ttccgacagt aaatatttcc ttatggctgt ggcggaagaa aagttttctt
    78721 tcatataggg atcattataa tcgcttcccg ttctttctat cggtacggtt aaaatatcgg
    78781 atttctgtct gattaatgct ttttcatatt ctattgccag aatatcattg gaggatttta
    78841 atgtttcgga caacttataa atatctgtat ccgatttatt cagtaatttt atcaaagcat
    78901 gctgtctggc tttgggaaaa gacagacctt ttgataactc ggctttaagt atctctttgt
    78961 attcatttga ttcgtaagcc aacagcgagg atatcttctt gacattatcg ttatcggatg
    79021 atttaaaacc gtaactgata tagtcgatta taccgatatt attcagtata taaacagcat
    79081 tgtcggcata tgattcagca gatgcgcaac agaacagggt cgggtattcc aataccatgt
    79141 ccgcaccgtt ggatatcgcc atctgcgccc gggcatattt atctaataat gccggttctc
    79201 ctctctggac gaagtcggaa cttaagagac atatcacctt gatattgttt ttgaataatt
    79261 tcttaatctc ggttatctga tgtaaatgac cgttatggaa aggattatat tccgatatga
    79321 tgccgacgat attcatctat tttgtccttt ttaaaaacta tacattaaat ctgaacagaa
    79381 caatatctcc gtctttgaag acataatttt tgccttcact tctgaccagt cctttttctt
    79441 ttaccgcatt atatgagcct aaacgcttta tatcatcata agtgaatact tccgccctta
    79501 taaaccctct ttcaaaatcc gaatgaatct taccggcagc tttgggtgcc gtagtattat
    79561 ttcttattgt ccatgcccgg acttcctttt tcccgacggt aagaaaactc atgaggtcta
    79621 aaagcttata gcctttgaca ataagtcttt ccaggccggt ttcggtaatg cccaaatccg
    79681 ccaagaatgc ggttttttca tctttatcca aagcggaaag ctcttcttcg aattttgcac
    79741 agaccttgat aacttcatta ccttcggctg cggctatttc cgttaataat tttgtgtaat
    79801 cattgtcttc cgacattccc tgttcatcga cattggcaac atagaggata ggcttcaagg
    79861 ttatcaggaa aagatcactc atatatgctt cctgatcggt attctcaaat ttaaaggatc
    79921 ttgccatttt tccttccagc aggtgttctt ttaaatcatc atagaatttc agcataggaa
    79981 tatatgcctt atcaccggat tttgcggttt tagcggtttt ttctattctg cgctctatca
    80041 tatctatatc cgcaaatata agttccagat taattgtttc cacatctctt acaggattta
    80101 cacttccgtc gacatgcaac aaatcagcgt catcaaagca tcttacaaca tgacaaagag
    80161 cgtccacctg ttgtatagcg gctaaaaact tgttacctac accctcacct ttattggaac
    80221 cgcgcaccag accgggaata tcaacaaatt caatggtcgc ataggtggtc ttatcgggct
    80281 tgtaaatgct tgcaagaaaa tccactctct catcgggaac attaactatg ccgacattgg
    80341 gctccaatgt ggagaacata tagttattgg tctgcgcctt gttgtgtgtc attacattaa
    80401 atattgtact tttacctaca ttgggtaatc caactattcc aagtttcata aaaacctcgt
    80461 tgtactattc ttggctgatt atatcacacc gaacatatat tgaacaaaat atcttatata
    80521 tgaaagcttt aaaagcatct ttaacggtaa tagctttgac attgaatatt tgctatgtta
    80581 aacttcagat atatccgaat caaggggtgc agtatgaaag aaaagattaa agtaggtatt
    80641 atcggaacgg ggaatatcag cacatttcat atggccggct acaaacgtct ttccgacaga
    80701 tgcgaggttg tagcggtatg tgatattgat gaagaaaaag ttacggcttt tgcaaagaaa
    80761 cacgacgtac cgtactacta caccgattac aataaaatgc tatcggcaca taaattggat
    80821 gccgtaagtg tatgtacatg gaacagtgct cataaagacg caacaattgc cgcattaaac
    80881 agtaaggcaa atgtgttatg cgaaaagcct atggcaatga atacagccga ggctctggaa
    80941 atgcaaaaag ccgcaaaaag aaacggcaaa ctccttatga taggttttgt aagacgattc
    81001 ggtaatgatg caaagatact caaatccttt attgataacg gtacattggg agatatatat
    81061 tatgccaaag ccacatattt acgtcgcagc ggttgtcccg gagggtggtt caaggatatt
    81121 agatatgccg gcggaggccc gttaatagac ctgggtgttc acgtaattga tctggttcgt
    81181 tatctttccg atactcccaa gccggtatcg gcatacggag cggtatacga caatctcggt
    81241 cccaacagag cggaaggacc caaaggatat gtcgtcgaag atagttcgaa atatgtattc
    81301 agtgtggaag acatggctac cggactggta agatttgaca acggacttac aatgtttgtg
    81361 gaggcttcgt ttaatttgaa tataaagcag gataccggtg taatagagat attcgggacc
    81421 aaaggcggtg cgaaaataga tcccggtata gaattttatt tagatataaa cggcaggttt
    81481 gtggatgtca aaccgcatgg tgattccgct ttgagtttta ccggtctttt tgaaagcgag
    81541 atagaacatt ttacggattg tgttatcaat aaaacgccat gccgtgctac agccgaagac
    81601 ggtgtaatgg taatgaagat tatcgatgcg atatatgaat cggcaaaatt gaaaaaggaa
    81661 gtaagaattg attagttttg tttatacaaa atattcaaac tgctcaattt ggtaaaggga
    81721 aattccgatg aataagatac tggttgttga tgatgatcaa aacatatgcg accttctgac
    81781 actttatttg gaaaaagagg gttatacggt tgtctgtgca aatgacggat tggaagctct
    81841 ggaagtgtac agagatgaaa atcctgattt gattattctg gatatcatgc ttcccggaat
    81901 ggacggtaag gatgtatgca aacagataaa gagaaaagac gatgtgccca ttctgatgct
    81961 gagtgcaaag gcagagactt ttgacaaggt tttatgtttg gaattgggtg ccgatgatta
    82021 tgttgttaaa cctttcgaag caaaagaact ggttgcaaga gttaaagcga ttatacgtag
    82081 gaccaagggc aatcaaagcg ggtattccga tttgaagttt accgatcttg tgataagtaa
    82141 gaatacatat accgttttac tcaaagataa acagattgag cttcctctta aagaatttga
    82201 gttgttattt ttaatggctt ccaatcccaa ttcggttttt acaagagata tgcttttgga
    82261 gaaaatatgg ggatttgact ttttcgggga ttccagaacc gtcgatgtgc atataaagag
    82321 gctgagggag aaaataaatc ttcccgatgt caactggcag ttgaagaccg tttggggagt
    82381 aggatataag tttgaagtat tatgaagcag aacgttttat ccacgacatt aagaagattt
    82441 gtgctttata tcattataat agtatctctt ttttcaatca taatctatta tcagacctgg
    82501 ttcggcatac ttaataatca gagtctgact gcaaagaata tatacccttt ggttaaaagc
    82561 gaactggaac aaagaaagat acttctctat tccaatctta catccagtgc ttttaat
//
LOCUS       DAOSOM010000006        70228 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_412_length_70228_cov_6.706882, whole genome shotgun sequence.
ACCESSION   DAOSOM010000006 DAOSOM010000000
VERSION     DAOSOM010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 70228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 70228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70228
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_412_length_70228_cov_6.706882"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="PLF14_02625"
     CDS             complement(<1..550)
                     /locus_tag="PLF14_02625"
                     /inference="COORDINATES: protein motif:HMM:NF014642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HOT70225.1"
                     /translation="MNIPENYDATPHSISVNTIIERYCLDKMYVPENIDKKIIDSAEV
                     YRPGLQLAGYLEGFCPERVQLLGRTEMNFLGTLSREERQKRLDAYMHESIPCIIISNS
                     QPVYDEIIKSARLKRVPVLRTYEGTAVFLAMLISYLNLELAERMTIHGELLELYGVGV
                     LITGHSGVGKSETALELVKRGHR"
     gene            complement(528..1295)
                     /locus_tag="PLF14_02630"
     CDS             complement(528..1295)
                     /locus_tag="PLF14_02630"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOT70226.1"
                     /translation="MDFIKAVNAVLDAGALMKDATYRTNAVKKGDKDYVTACDIAIQK
                     SIAQALRQHYPDIPLIAEEKDNRDILSDSVFILDPLDGTTNFLHGYTPSCISLAYVYK
                     EQPVFGIVHDPVADATYIAEKGKGAQLNGKPIRVSNVKNIENALITCETSPYERGDAD
                     MHFDVLKKVFLACMDIRISGSAALDICYVAEGAADAFLTRNLSPWDVAAGICILTEAK
                     GVCTDWKGNPPSMRYKSDILASNGLLQEVLYEYTGKL"
     gene            complement(1295..1582)
                     /locus_tag="PLF14_02635"
     CDS             complement(1295..1582)
                     /locus_tag="PLF14_02635"
                     /inference="COORDINATES: protein motif:HMM:NF019749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HOT70227.1"
                     /translation="MKKILILFLTAVILLTLTACQTYLFGVYVSEETGRKYEFMNNEF
                     TCTENDNEISGTYVIKNKTITFKYDGKEDTFSYSRDGAAAVIDGVVYIRGQ"
     gene            complement(1618..2991)
                     /locus_tag="PLF14_02640"
     CDS             complement(1618..2991)
                     /locus_tag="PLF14_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70228.1"
                     /translation="MKFTKIFASSVATLDSNVYNGGGTDVTAALQRVLDKAGEDQGIH
                     LIMDGAALITGLDLKSNTTIECLSKDCGFFLKDGSDRSLIDLKERSYTKLLTRNVSLI
                     GGTYNHNNRGQQHHVPITEEKYRFGIEGISDDFGAYRMVVAFEFFGVQDLIMRDITIR
                     NQRAWSVMCVNFKNVTIENIYIDLPNWMRGQNQDGFHFWGPGQFLTMRNIKGRSGDDF
                     IALGPDEHDLVSSITDVLIDGVHLDYADQAIRMLCRSKGHLDRVTVRNVTGTYRSYGF
                     FINPWFPGDGFGNYGHITFENIDLRPMDHVYPYRTATLFDIGGNFDCITFKNIHHQNP
                     SDNRPLWVFGLPFHENDLNYAPDCRPRIRNVVIDGLTVIQAASDVKVDGYIHVYDRVE
                     NLFLKNVIVSSDEGAVKTDAFITFRKFKNCERYGAIGNLVTHDIYMPNVENLTDNPSQ
                     VECMCDT"
     gene            complement(3071..3466)
                     /locus_tag="PLF14_02645"
     CDS             complement(3071..3466)
                     /locus_tag="PLF14_02645"
                     /inference="COORDINATES: protein motif:HMM:NF024861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HOT70229.1"
                     /translation="MLVVIDTNILVSALWSRNSNPAKIVSMILRGVLVPCFDYRIITE
                     YREVLKRPGFGFSEGEINSLLGWFESNGHSVVAKPSDIDFTDEADKMFYEVAKHCGAV
                     LITGNLKHFPKDRKILNAADFLKEYADGF"
     gene            complement(3470..3745)
                     /locus_tag="PLF14_02650"
     CDS             complement(3470..3745)
                     /locus_tag="PLF14_02650"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOT70230.1"
                     /translation="MNFYTVRDLRTTPKSLWEDLVTNGEVVITNNGRPTAIMLDISDG
                     SFEETLKAVRQAKAAIAFNSMRAKAAAKGYMSDEEIEAEIAAARRGE"
     gene            3831..4019
                     /locus_tag="PLF14_02655"
     CDS             3831..4019
                     /locus_tag="PLF14_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70231.1"
                     /translation="MNIGYTFIEVVMMLKSITPSTKENCIEETRENNLSEKLDSFLAK
                     TDYVNSGGSFTRDEMNER"
     gene            complement(4353..5075)
                     /locus_tag="PLF14_02660"
     CDS             complement(4353..5075)
                     /locus_tag="PLF14_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70232.1"
                     /translation="MADSKYLNGLKGKEKFSRKEMLESFRREGYSLSDASFYKTLEGL
                     VHNGNIIRESRDVYFVPDGRTADYTHTYSELAKEVANIMTEQYPYLTYSVFELVQLND
                     FVNHQIAHNVIYLSVEADITGFVFDTLKEYYPGKVLINPTVELYHQYWSDNMIVIVKL
                     TTEAPKGKEIVWHARLEKILVDLFAEPLIMESVSESEYPAIYKDAFTRYVIDKKALFR
                     YANRRKVDKKISEFIRENDIKL"
     gene            complement(5318..6946)
                     /gene="groL"
                     /locus_tag="PLF14_02665"
     CDS             complement(5318..6946)
                     /gene="groL"
                     /locus_tag="PLF14_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160286.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOT70233.1"
                     /translation="MAKQIKYGIDARQALETGINKLADTVKITLGPKGRNVVLDKKYG
                     APLITNDGVTIAKEIELEDAFENMGAQLVKEVATKTNDVAGDGTTTATLLAQAIVREG
                     LKNLAAGANPMILKKGIEKAVDAAVSGIVENSSHIKGKSDIERVAAVSANDGEIGKLV
                     SAAMEKVGNDGVITVEESKTMGTNLEVVEGMQFDRGYLSAYMVTDTDKMEAVLDDAYI
                     LLTDKKISNIQEILPLLEQIVQTGKKLLIIAEDVEGEALATLVVNKLRGTFTCVAVKA
                     PGFGDRRKAMLEDIAILTGGQVVSEELGLDLKETTMDMLGRARQVKVQKENTIIVDGA
                     GDAAKIKARVASIKSQIEETTSEFDREKLQERLAKLAGGVAVIQVGAATEIEMKEKKL
                     RIEDALAATRAAVEEGIIAGGGAAFVDALPKVNAVIRSLTGDEKTGAQIVARALEEPL
                     RQIVINAGLEGSVILAKVMSSKKGVGYNVLSEKYEDMIEVGIVDPTKVARTALQNAAS
                     VAALILTTESLVCDIPEKEPPMPAGNPGMGGYGM"
     gene            complement(6964..7248)
                     /locus_tag="PLF14_02670"
     CDS             complement(6964..7248)
                     /locus_tag="PLF14_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOT70234.1"
                     /translation="MVIKPLCDRIVIRMLESEETTKSGIVLPGSAKEKPQVAEVIAVG
                     PGGFVDGNKVEMELVVGDKVLISKYAGTEVKLDGVEYTILRQSDVLAKVV"
     gene            complement(7421..9073)
                     /locus_tag="PLF14_02675"
     CDS             complement(7421..9073)
                     /locus_tag="PLF14_02675"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008395088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOT70235.1"
                     /translation="METYPILKGEIVESTTKFIFVTGGVVSGLGKGITAASLGRLLKA
                     RGLKVAAQKIDPYINVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDENLNKYSN
                     LTSGKIFWNVLNKERKGEYLGETVQIIPHITNEIKSFIYRVGKKTNANVVITEIGGTI
                     GDIESTYILEAIRQISLELGPGNCLFIHVTLVPYIASSGEYKSKPTQHSVKELHSMGI
                     SPDMIVLRCSDHLDDSLKKKISLFCNVKEDCVIENLDMDVLYEVPLELEKLDFSSLVC
                     RELGLVTKDPDMTEWTAMIERLKKIEDEVVIALVGKYIQLHDAYLSVVEALKHAGLEN
                     NVKPVIKWVDSEEVTAENAQEVFADCHGIIIPGGFGIRGVEGKIIACNYARVNNLPYF
                     GICLGMQIAVVEFARNVCGLTDANSREFDERSHHKVVDIMPDQYVSMVKGGTMRLGAY
                     PCKIKDGTLMHQAYKTDEVSERHRHRYEINNEYRKQFEAAGMVISGTSPDDILVEAIE
                     LPKNDFFLGVQFHPEFKSRPNRAHPLFTLFIKHAVHKKGKKK"
     gene            9463..11022
                     /locus_tag="PLF14_02680"
     CDS             9463..11022
                     /locus_tag="PLF14_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOT70236.1"
                     /translation="MAYFLKQTKQKGRTYLSIVESFYHPKKKGTAHRVYKSLQSVETW
                     IEKGIENPVAHFQKEVDELNKVKPKEKEKKISDIPPVVHLGYFPLKSIMEKLEIEKNG
                     DYLKLTKNLDFNLYHLISSLTYARVVEPCDANEAFYDILPLLDFSYCFSHEQMLEGLA
                     FLGQNYEKFAKIFTLQTKEKFGIDTSATYFDYDNFYFNMTEERNLGDGTGKKGSLFRY
                     AILTDKNNIPIGLNFLWGDVSKKYLLKDIIQRLNATNIPVKKTVFVIEEEEGSISFDF
                     NCGYLYSPPLPLSEKTEKERILSNKGYSEVKDSYGNLLYRHKSYIDRFPYTVSIKGKE
                     DNVMLEEKRLITYAPYLVEEDKLIIKALAECTDIYSFKEETDEALSFAGYRLFITSDV
                     NMSDRKIYDTYYYFRHIVDTFRSMESDINGYSPTLETEDCIKGYFLICYIALLLERLF
                     CFKVLEDKYSVSELSYFMENFKLVRTETKYLNMARYTAFLDRLSNILKLPLTDYYLTE
                     RQVKRIRDYTL"
     gene            complement(11042..11575)
                     /locus_tag="PLF14_02685"
     CDS             complement(11042..11575)
                     /locus_tag="PLF14_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70237.1"
                     /translation="MAFLVRKLIKMSELFSLCERTEIKEIYADIPTTEFRTKNGTLST
                     WHINSLDDIEDAILAIAFTSSDITKMDFIIIDTDILSKNSLEYKKTYAGQEIAIVDLQ
                     DTHYDIVNITIEKLINCTRVYQTVVEADPDAETYIIRYTAGDIRELLKKSWEEKRVDE
                     KKVPEKLKEQFNKLKVN"
     gene            complement(11575..12897)
                     /locus_tag="PLF14_02690"
     CDS             complement(11575..12897)
                     /locus_tag="PLF14_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70238.1"
                     /translation="MFVPLNYQLSIFGEYSAIEPSPEIIIDFMSKMNQEIQQVLLPIV
                     VNSQPISEPSSKNAVRYNIGFISQDQRYSITILDDRIDINYSNLDESDIDMKDFFILA
                     TKVFTVIIDYFSISSNRLAMNILQVHEMENVHKLEKCGKSLLKSAAYYKDKDFVEWAM
                     RTNAHTDILINGFSETLNVITDISYGQHVKEQKAGVVFHIDINTLQQNQDMRFKKKSI
                     ESFIRKVMPIASELIVDNERLLQMENSTKDYKNVATVGETNILYFPNSSRFQSTIRSV
                     VSKTDSSATFGKEIFGESKVRKNIISRYINEIKILIRQDEFIDGEFSQSELYMDDAYD
                     KGQIDYVTDALMEIYTSSLNEPHMLEGILTMISSVPYEIVYPKGQIIAAGLLENKELS
                     VRDKAIQCFEKWNSKKGLIFLKNIHCTPKWLQKYTDRVIMHIERDGTE"
     gene            complement(13158..13796)
                     /locus_tag="PLF14_02695"
     CDS             complement(13158..13796)
                     /locus_tag="PLF14_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HOT70239.1"
                     /translation="MNKLKKIYNTLYSHYGNLNWWPADTPFEVIVGTILTQNTAWSNV
                     EKAIERFEGDLSPERVMSLSMEELQEIIRPAGFFRQKAQYLKAVTEWFMGYDCNIDLI
                     RECSLSDIRAELLKVRGVGNETADSILLYALCLPSFVVDAYTMRFFRRFPVDAGNTYM
                     EVKNYCEKELPSDIEIYNRFHALIVHNGKEHCKKKAECAGCPLEKYCKKISS"
     gene            complement(13874..14839)
                     /locus_tag="PLF14_02700"
     CDS             complement(13874..14839)
                     /locus_tag="PLF14_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70240.1"
                     /translation="MKPIFIIIIVIVVLAVIGGVLYLHFRNMIKMPGEVTDKTKKNAP
                     KTIKSEKISKFDAEFVYSNECGELFYHFKAKRINRTVTELTGGLVKMEKTIHTGPEFL
                     VELQKIIDKYNLASKNGIYRVTSGLPNDPTRFLCRYRSCESIRFCVDNDSKSAWMKEI
                     YELFSTEFGKKGERKDFLSDSEMTRFYFRHGGMAMPQIYSFTIEKKGDKYLFKANFSD
                     PENSYKEAEVVWDKEQDQDIVKRFYYWVLRIYYGNQIYLWDGFDGNNRFVKDGTMFNM
                     SVDFENGEKIRADGNNSFPDKYYKAHKEIEKLLEEIIKVYQERGK"
     gene            complement(14890..15861)
                     /locus_tag="PLF14_02705"
     CDS             complement(14890..15861)
                     /locus_tag="PLF14_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70241.1"
                     /translation="MKPLYIIIIIAVVIILIVVCTVLFFHFRDQERMPGQVRDRTKKN
                     APKVIKSKDIKSFDTYFIYADEYGERFYQFSAVKKSNTEVELTLGPGDIEDVTHVGCE
                     FLVSLQEIIDKHKLASDNGVYRVTAGLPVEPTSFLCKYESGESIYFYIDGDSYSEWMK
                     DIYELFAIQFGGIGERKDFLSESEMTDFYFSHSGMGKDYIYSLDIRKRGEDYRLRADF
                     FDPENSYNEVKPVWDTKEYSDVVEGIYNQVIRIYSENHIYMWNGFDVCNNDILDGTEF
                     DMSIIFANGKSISACGSNSFPTGYNEVKDEIEKLMDEIIKIYHEKSK"
     gene            complement(15950..18205)
                     /locus_tag="PLF14_02710"
     CDS             complement(15950..18205)
                     /locus_tag="PLF14_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008700588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HOT70242.1"
                     /translation="MNEILQLKGRFEQKDSGNRPGHSNIPKNKEVKVSRLIDLKNDLV
                     KVREFWEKESLRIKPLVSVYYTCVVAKSNRMKGILESGSRKNNQSIVGAKFSFDGSPK
                     HIITHCVSDKTLIDAINNLKRVIKIVEETFGEEITYDKIDDINRDKYKSIFRSRDAAG
                     LSKTRLVNTIVDAYYIENFGIERDTSDLQDDAIITIYDTGTKTSDILKQVGIDLLDVR
                     IIDETTILLKPDQYRLLKEKAPYLISMAVTDISKLEKVDMSSTYETSTLIPHPGNEPT
                     IGVIDTMFDENVYFSEWVEFKNMLDSNIPLEGKDYDHGTMVSSIIVDGAGINPDLDDG
                     CGRFKVRHFGVSKGGQFSSFTILRAIKEIVATNRDIKVWNLSLGSAMEINANFISPEA
                     AILDKIQYENDVIFVVAGTNKPKDSNLKKIGAPADSINSLVVNSVSFDDEPAEYSREG
                     LVLSFFNKPDVSYYGGAGEKGIRVCSPYGAKTVYGTSFAAPWITRKVAFLIEVLGFTR
                     ELAKALIIDSAARWDSKPYSSTLIGYGIVPIHIDDIVMTPGDEIKFTIDGISEKYDTY
                     NYNIPVPEDRGKQPFVSKATLCYFPNCSRNQGVDYTNTEMDIHFGRLIKTKKGKIKID
                     SINDNKQSDEENLLLYEGTVRELFRKWDNVKHIRENVKTPTGRQKKPKEKLENGLWGI
                     SIKTKERLNTEDGENLKFGLVVTLKEINGVNRIQEFIQQCQFRGWLVNRVNIENRIEI
                     YNRAEETIHFD"
     gene            complement(18213..19247)
                     /locus_tag="PLF14_02715"
     CDS             complement(18213..19247)
                     /locus_tag="PLF14_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016361302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT70243.1"
                     /translation="MKKANIINLIKYYAENNDVGFRNEAYQIADIFDKMGDYQLSEYI
                     MALLSDANTFVPQINEDDLTFVRKIEVTGDPLPLPEKILEDVKGIINAVGHRIGVNKF
                     LFQGAPGTGKTETVKHIARILNRDLFIVDFAYVIDSKLGQTGKNIAKLFDEINNLVAP
                     EKVVILFDEIDALALDRTDSKDLREMGRAMTSVLKGLDELNERILLIATTNLFEYFDK
                     ALIRRFDFVIDFNRYSREDLIDIAEIILNHYLSKFDNAGRNIRLFKKIISSMEKIPYP
                     GDLKNIIKTSLAFSSPDNEFDYLRRLYASVCEEGGLELKVLQDKGFTVREIEILTGVS
                     KSQVSRELKE"
     gene            complement(19634..20329)
                     /locus_tag="PLF14_02720"
     CDS             complement(19634..20329)
                     /locus_tag="PLF14_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70244.1"
                     /translation="MENAIRELSETIQQVNMHFNWPSAISAVCSVVSLLAIVVLLKER
                     AEKKRPYLQVSLELIRGTLVCIVIRNVGVVPAVLKKVQYNESFIKQLSHDAQKRCLVG
                     KEINVSIHPKQQWVLCLGETFSKVVDYKNKMLEVTYTYVSKGKQKKFTEKEIICFDDY
                     SIFLEYISEIDELRSEIKELIINLNNINETLQTISKTNIESVQLEKCANIGEKCNRYV
                     PRIKESKVPKDVT"
     gene            complement(20384..20551)
                     /locus_tag="PLF14_02725"
     CDS             complement(20384..20551)
                     /locus_tag="PLF14_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70245.1"
                     /translation="MHNKPELEVEWKRKQKNHTDYYVQNENELLVNAYFERFCTIKQS
                     DKKSGSDVRKD"
     gene            complement(20651..22480)
                     /locus_tag="PLF14_02730"
     CDS             complement(20651..22480)
                     /locus_tag="PLF14_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008854427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing HNH endonuclease family
                     protein"
                     /protein_id="HOT70246.1"
                     /translation="MAKNIEPKLKKIGEYLKLEGDSKFLVPEYQRAYSWEEKQCDKLW
                     QDIEDFIDSGGTDPYFFGTIIISCQEEDKNLSLIDGQQRTITFILLLKALLIRLNQSI
                     EETKGDEDSQRLTRALEMKRDRIIKILYKAKDEDIYDILANFENVQTADILENLSINE
                     QFSSDLKTILNSHDFKKAENEVKKIKYKQRNNKYTNNFRNFKFFYTKLLERSESNINV
                     FSEYILDKSEIIEIRSWNVEQAITMFNSLNSDGMPLLDADIISAKLYSNSGDNREDFV
                     EKWTELKGLVSDLEQRRIVDIDSILMQYMYIKRAIDREYISETGAVSVTTPGLRRYYT
                     EENKELLKNSLGLTAQLLKIAKTWDIIKYYPTVQMSAKFNENIKLYLISYLYRYELDE
                     ITENLVVDFLHYLLKLFSVLELEDIGYSSAKFKTFLFGLNIKVVDKNTEIEEIKKDIK
                     SHINKNWDKENIEKEILDYNKNPLVYLSEYIYSKKIDKKFKLPEKYEIEHIMPRSGKN
                     IVQIRIDAGLSDEEEFLSVVNKLGNKMLLEEDINRSIGNAWFRTKIQSSVKEKKGYKD
                     SMFLLAKNMIDTYRDSSEPVWTVEDINKRTEKISKEISDFIFS"
     gene            complement(22561..25497)
                     /locus_tag="PLF14_02735"
     CDS             complement(22561..25497)
                     /locus_tag="PLF14_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004027190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HOT70247.1"
                     /translation="MGIRTQKAESSLEKQIIEYLSSESVAYEYVEPEKMKLVYNKRHA
                     VDEERLFRFIEKTQPKAYTDHRLGTDAGRQKFLNTLSSNLHTQGVAKVLKDGIKMYPT
                     GIIFFYHALDKKRPDSFKEFDSNTFSVTNQLTYSEKNPGLELDICIFINGLPIFTMEL
                     KSKASSTGWTYRDAEDQYKADRDPKETLFSFKRCIAHFAVDENNISFTTRLAGKSTFF
                     MPFNKGDGKGGAGNPIGTGTMTDYLWKVFLDRKMLAKLIVDFIYVEKKKDKDTGKTKE
                     TLIFPRYQQWRVVERLIEDIEKNGVGKKYLIQHSAGSGKSNSITWLAYRLVKAEKDDK
                     TLFDSIIVVTDRRNLDRQISDNIRHFMSVSSNFAHSESSKDLHDLLVDGKKIITTTVQ
                     KFPYIIETIGTKMKGKNFAVIIDEAHSSQSGKAAASLSTAISGKYHIEDDMDSEDFIN
                     AIIEARKMPENASYFAFTATPKAKTIEMFGSVFDLYSMKQAIEEGFILDVLKNYTSYE
                     NYYKVYKTIEDNPVFDSKKAARKIKTYVEGQAFPIRQKADEMVKHFIHNSSNKIGGKA
                     KAMVVTSSIMRAIEYYHAIKDILKGYNSPFKALVAFSGEKEYMGKKVTEASLNGFSDK
                     ETATVFKGNEYRFLIVADKYQTGYDEPLLHTMYVDKILNDVKAVQTLSRLNRSYPGKI
                     DTCVLDFANKPEDIQEAFQPFYKETKLIEGTDPNKLYEILGRLDALCVYEEDQIERVV
                     DMFFKDQPREFIDPIMDNCVERYKELKEEEQVEFKSGVKAFIRNYNFLSAILPIGQVE
                     WTKKCIFFEFLVHRLPTPRVDDNIDELIESIDLESYRLEKKNEIDIALSNVDGELEAK
                     QFSTVSISEAQFEYLDTIIANFNDIFGDIDWNDKDNVARQIRELPNMVAKDERISNAI
                     KNSDERNIRIEYDVVLQDVMRSMMKDNMELFLQFTSNPQFKRWLSDTVFTEIMNKGEY
                     YFEQGQ"
     gene            complement(25500..26885)
                     /locus_tag="PLF14_02740"
     CDS             complement(25500..26885)
                     /locus_tag="PLF14_02740"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOT70248.1"
                     /translation="MRYKAYKKVDLPWLEEIPEHWEIAALKRFTDIYTGNSIPDEKKD
                     HYTKNDSARPYISTKDIRKDNNTIDYNNGMYIQKNDLSFKIAPKGASLVCIEGGSGGE
                     KIGYLEKEVSFVNKLCCVSSEKQVNDKFVFFLLLSNQFKHQYKMSVTGDRNGVNLGKL
                     GTFNFALPPKEEQEQIAEFLDWKINEIDRLIGLERAKINSVENLKQVLINKYLFSGLD
                     KNVPKVNITTDYTNSIPESWKFESIKRNFEIVKNIVGEEGYNILSITQKGIKIKDISA
                     NEGQMAQNYSNYQFVEVGDFAMNHMDLLTGYIDISKHFGVTSPDYRVFRIKNDKKIYP
                     EYYLLMFQLFYKRRVFYKLGQGAARMGRWRLPAKKFLKMYVPVPSFDEQKRIVDKIKS
                     ELLSLNKYIENLDNTIQALKNLKKTLISDVVTGKVDIRNIDIPEYEKVQILDDTLEED
                     TEVEIDFEEED"
     gene            complement(26875..29067)
                     /locus_tag="PLF14_02745"
     CDS             complement(26875..29067)
                     /locus_tag="PLF14_02745"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006806061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HOT70249.1"
                     /translation="MKNYKLSTADYNKLVNFIWSIADDCLRDVYVRGKYRDIILPMTV
                     IRRFDAILEPKKKEILDLKETFIKNQAENLEAAMALAIDLPFYNLSKFTLKDLKSETS
                     AQNLKKNFEDYLNGFSPNVREILDKFKFYNQLDTMTEAGILGSVIEKFVSTEINLSPY
                     PVLNSQDEVIKPGLDNHTMGVLFEELIRRFNEENNEEAGEHFTPRDVIELMADIAIIP
                     VADKIKDAPYTLYDGAAGTLGMCTVAAEKMQDLAEKKGKTISTHIYGQEVNPETYAIA
                     KADMLVKGQGQDSDNIYFGSTISSDFLAGHTFDFMLSNPPYGKNWKIDLEKIGAGESK
                     NLKDNVIDSRFVTTHKGDGDFRMIPDVSDGQLLFLLNNISKMKDTEMGSRIVEVHNGS
                     ALFTGGVGNGANNARKYMIENDLIEAIIELPENMFYNTGISTYIWILSNRKEERRKGK
                     IQLINASSIKTSLRKNMGKKNCEFSQYDQKKILNLYLDFEENEYSKIFDNDEFGYYQV
                     TVERPLRQAVRVDDETLSEFNAILKGLGVLDGEFDAKALKEYKDKGVKSTKGSKTELA
                     DTDKMKAYLEIMDTLKSAEDYLSYTEFEEKFNEKAKGISGLTLNNLNKTSLLDLFVKK
                     NEKAAITKDSKGNLVPDADLRDTEQIPLTHDGGINAFIEKEVIPYHEDAWVDEASIQV
                     GYEINFTKYFYEPKKLPSVKEIVEEIKSLEKESEGLMDTILEGLDYEI"
     gene            complement(29399..29653)
                     /locus_tag="PLF14_02750"
                     /pseudo
     CDS             complement(29399..29653)
                     /locus_tag="PLF14_02750"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(30329..30910)
                     /locus_tag="PLF14_02755"
     CDS             complement(30329..30910)
                     /locus_tag="PLF14_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70250.1"
                     /translation="MHEQVFLICIGALISSFAYVFHKLVDRWLDNTGDVTIYRKLVYE
                     KNSDKRPSGIYNMNGEIVMRLPIWIEIQNMKKTPVIIRDFGVVLYKKGRRIKKMKQIN
                     YQKDNTKKDSVLVYYGDNGRYSFIIPSESIRAFDLLFVLKKTECPSDFDEINITYYNQ
                     KNKFIKVSLKKVEKSWALQQFDVDEDWVEVNKF"
     gene            complement(30912..31520)
                     /locus_tag="PLF14_02760"
     CDS             complement(30912..31520)
                     /locus_tag="PLF14_02760"
                     /inference="COORDINATES: protein motif:HMM:NF037930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70251.1"
                     /translation="MARSQIIKDLIDGDKSITVSLQKLLVIVDELENKELKEWAKNEL
                     NGYGADEDIPDYRKNLPFAILYSGMNGSFLVKNQPLVINSFGDYADEVRKLNIITNSI
                     LELENLKGGTLQYDLTKYAEVVKKNMGISCISIVLKYGYNVADSILSSVKTKVLESLL
                     YLEKEFGLLDELFIADSKLDKSKIEKTNHNINSIIFSDGVRY"
     gene            complement(31651..32676)
                     /locus_tag="PLF14_02765"
     CDS             complement(31651..32676)
                     /locus_tag="PLF14_02765"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HOT70252.1"
                     /translation="MKVFISWSGTRSRKVALIFHDWLPSVIQSIEPFVSSEDIDKGAR
                     WNTDIARELKESTFGIICVTKENLLAPWLNFEAGALSKTMDNTYVAPFLFDVKPSDLK
                     NSPISQFQATSFNKEDLKRLLGTLNRAAENSLSDQQLAKTFEVWYPTLDKSLNDLHLE
                     PTENSEDNLETVESDVLEEILEMSRNTQRLLGNVDARIYKNNEQFQKMVEEVMSKSYR
                     VLDVDAMHYSCRMSSVIAEEIFYMLRSEKDFDNIMCYDLMVILSLYRDDFPWLYDVAS
                     ELERIIESDIPHESKVKIIDRYKRILNFIVDHPKTRELFRYNKETLMILRELSMKLID
                     ELTYKIL"
     gene            complement(32980..34305)
                     /locus_tag="PLF14_02770"
     CDS             complement(32980..34305)
                     /locus_tag="PLF14_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT70253.1"
                     /translation="MKESQNIEWKESWRDEYLKWICGFANAQGGKIYIGTRDDGSITG
                     VKNAKRLLDDLPNKIQSKLGIVADVNLLTEGEHEYIEIVVSPWAFPVNYDGLYYYRSG
                     STKQLLRGNALTSFLMEKTGRKWDAATITNVGIEDLDKESFDIFRREALRSGRMTKED
                     LDISNAELLEKLDLIVDGKLKRAAALCFYRQPERVSGGCYVKIGKFEGSEILYQDEVH
                     GSLIIMADRVIDLIYLKYLKAAISYYKDTRVETYPFAREAVREAVFNAIIHCNWAENV
                     PVQIRIEDDVMYVSNCSMLPFGWTAETLLGSHVSKPYNPDIARVFYRAGYIENWGRGI
                     QKIREACVAHGAEDPEYIVHGGDIMVKFKALQRAIVTSNEPNVTKDVTIEEMILDLVR
                     KNKAITTTEMAENLSVARRTIQRGLDTLKEKGIIERKGGRRYGYWEILK"
     gene            34764..35303
                     /locus_tag="PLF14_02775"
     CDS             34764..35303
                     /locus_tag="PLF14_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HOT70254.1"
                     /translation="MITQKEYNKARKATLEYLDKAGIVVTEAEKERIEVADFGLGDLK
                     NTGLQILTYVNTKKVCAKELVLTPFQTCPEHRHPPLKGSDGKEETFRCRYGEVYLYVS
                     GPATKNRKVDPPEGVYTVFHEIILKPGDQYTLYPDTLHWFKAGASGAVVSEFSTSSHD
                     ESDVFTDPDINRMSGVEEK"
     gene            35321..36565
                     /locus_tag="PLF14_02780"
     CDS             35321..36565
                     /locus_tag="PLF14_02780"
                     /inference="COORDINATES: protein motif:HMM:NF019736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase"
                     /protein_id="HOT70255.1"
                     /translation="MSKLFIQYGAGNIGRGFIGQLFSQGGYRVGFIDVNTDIINRLNA
                     DKKYTVTILDNDIKEEIVLYDVYGIDGRDNSAVAEAIASADCMATAIGVNILPYIAPN
                     IATGIKLRYQRGITSPLDIIICENLIDADKYLSQLICKQLDEKETGYFTEHIGLVKAS
                     IGRMVPVLTAEQIKSNPTNVFAERYCKLPVDKDAFKGNIPDIPGIYPFSPFGYFIKRK
                     LYIHNMGHSLTAYLGALSGYKYIWEAIDDSRIKYVVLRAMLESAGAISSEYDMPLRDL
                     LLHIYDLMRRFSNHALEDTVDRVGKDIKRKLSENDRFIGALKYCLSIGIRPVYIPAGV
                     AAGLLFRAQKDDNSGELIKALEEKGVVYVLENFCGLSDNETYLNMITEYYKQLTDSFD
                     MEKFIYNLDERYGKINRESDIL"
     gene            36584..37534
                     /locus_tag="PLF14_02785"
     CDS             36584..37534
                     /locus_tag="PLF14_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOT70256.1"
                     /translation="MNDIACVGILVADCIAKPVKVIPDKGKLELVDSIGLYSGGCAAS
                     ASINITKIGKKAVIIGKIGNDGFGMFMKSSLESHGVDTRGLKVDANGETSSSLVMVTP
                     DGERSFLHSVGTNGTFNEKDIDYDIVKSSNIIFVAGTMLMPAFDGKDCALFLSKCKAL
                     GKTTALDTAWDSLGRWMKLLAPCMPYIDYFLPSYEEAVQLSGKTKPEDIADVFLGMGP
                     HTVVIKMGEDGCFIKSKLTGEKHFIDSFRVNAVDTTGAGDAFCAGFLSALVSGKSLYD
                     AGRFANATGAHCVMAKGASTGIKSEKEMEKFIADYDVGIL"
     gene            complement(37701..37994)
                     /locus_tag="PLF14_02790"
     CDS             complement(37701..37994)
                     /locus_tag="PLF14_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70257.1"
                     /translation="MSILKQVLIEELERNNHFIDSAEKEISQLPNGYLSKKNINNKTY
                     FYYQWREGEKIKSEYIKMKDVQNLDKKIKRRKELEKAILEARNENKRIEKVLK"
     gene            complement(38118..38681)
                     /locus_tag="PLF14_02795"
     CDS             complement(38118..38681)
                     /locus_tag="PLF14_02795"
                     /inference="COORDINATES: protein motif:HMM:NF025124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT70258.1"
                     /translation="MQIKIEDDVMYVGNCSMLPFGWTAENLLGSHVSKPYNPDIARVF
                     YRAGYIENRGRGIQKIREACVAHGAEDPEYIVHGGDIMVKFKALQSAIVTDTKVPDAT
                     TVLKNLNDVGHNVGHDVGHKLEPNERRRKIVDLLFENPNTTAPELSKVFSLNVRTIER
                     DLAKLTEEGFIEYIGSSMEGAWKVKKR"
     gene            complement(39072..40460)
                     /locus_tag="PLF14_02800"
     CDS             complement(39072..40460)
                     /locus_tag="PLF14_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013977532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HOT70259.1"
                     /translation="MDIKSIVKDLCAYDDEQEWFEFKENWFQPETLGEYVSAMSNAAA
                     FHYKKYAYFVWGVNNDTHEIVGTTFNQYCEHNKEPFQNYLARKLSPSINFSFEEETID
                     GNRVVVLVIPAAEEIPTAFKEKRYIRIGSSKAALKDYPKREIQLFKILDGRVETIETV
                     PSKYQELTFSKLFGYYGSRGIVLRKETFIKNLGLKNKSGEFNILAQLLSDNSHIPLRV
                     SIFDGETKGSPLFSVREFGNSCLLYSLDELLRYGDVLNLIQTDERNRIVERKEVPLFD
                     NKAFREAVINAILHNRWIDGNEPMFSVFSNRIEILSRGTIPPAQTMEGFFLGESVPVN
                     EKLSEIFLQLHISEKSGRGVPKIIEVYGKDAFTFRENSIVVTIPFQRIHNPQNAEKKL
                     GNNMGNKKPLNERRSRILAEMRNDPNITTAQLIVILNCAETTVENNIAFLRNNGYIKR
                     VGSRKSGYWKVL"
     gene            complement(40551..40991)
                     /locus_tag="PLF14_02805"
     CDS             complement(40551..40991)
                     /locus_tag="PLF14_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70260.1"
                     /translation="MKASTRASLSFSEGIFAVICVCHRKSMKFNAANVDSTRTHLNIN
                     NCNAPIKQVYHELVIQIGKKDDTGSATEIGEQAKLALDEYFQSFHERNQWVQSEKEQL
                     AQGMECYGFEWEHLSIIEFKKQERAKAAQQVQKRQRQRSRGWER"
     gene            complement(41172..42098)
                     /gene="guaA"
                     /locus_tag="PLF14_02810"
                     /pseudo
     CDS             complement(41172..42098)
                     /gene="guaA"
                     /locus_tag="PLF14_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015485001.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
     gene            complement(42238..42522)
                     /locus_tag="PLF14_02815"
     CDS             complement(42238..42522)
                     /locus_tag="PLF14_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70261.1"
                     /translation="MDENSKSSKIMKWAIVIFAIGFEGITFGMFMRLVYMNEQYMARW
                     HMWVWYVVGVAFLIPSFYLFYRANKQKEQERHEYYEDYFKEEEKKITIEE"
     gene            complement(42669..43445)
                     /gene="hisF"
                     /locus_tag="PLF14_02820"
     CDS             complement(42669..43445)
                     /gene="hisF"
                     /locus_tag="PLF14_02820"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007546375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOT70262.1"
                     /translation="MSVNKKGRIKRIIPCLDIDKGRVVKGINFINLTDAGDPVETAKA
                     YCEKGADELVFLDITASSEKRGNTLTMLKKVAEETDIPFTVGGGIRSIADFESVLKAG
                     ATKVSVNTAAVLNPDLLSKASTMFGREHIVAAIDAKKQEDGRYAVYINGGRKKTNLNA
                     VDWAKKCEELGAGEILLTSMDRDGKKNGFDLELTKSVSQAVSIPVTASGGAGSIKDFY
                     DVLTKGYADAALAASLFHFHELDITELKKALRDKGIAVNI"
     gene            complement(43467..44177)
                     /gene="hisA"
                     /locus_tag="PLF14_02825"
     CDS             complement(43467..44177)
                     /gene="hisA"
                     /locus_tag="PLF14_02825"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HOT70263.1"
                     /translation="MIIYPAIDILNGHCVRLYKGDYGQVTKYSDDPVKTALTFKEAGA
                     SHIHIVDLDGAKTGKSENIEVIKRICKETGLSVQTGGGVRTIKRIRELAAANADRIIL
                     GTAAIKNPSLVEEAVKEFGKKIVVGIDARNGFVSTDGWTDDSGKDAIETALFMQSLGV
                     QSVVYTDISKDGTLKGPNLDALKEMIEKTDMYVVASGGIKDMEDIREVKETGAYGVIT
                     GKAIYEGKIHLKELFTYS"
     gene            complement(44183..44767)
                     /gene="hisB"
                     /locus_tag="PLF14_02830"
     CDS             complement(44183..44767)
                     /gene="hisB"
                     /locus_tag="PLF14_02830"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOT70264.1"
                     /translation="MRKATFKRKTKETDVSVKLNIDGTGKYTVETGIGFFNHMLELFS
                     KHSLMDLTVKTTGDIEVDEHHTVEDTAIALATAMKEALGNKEKISRYGFASVPMDEAL
                     TNVTVDLGGRPYTVFICPVSGGRTGEMDFETVEEFVTAFASNLGANIHVNVQYGHNRH
                     HIAESIFKALARALRQAVSIDNRETGIPSTKGAI"
     gene            complement(44767..45819)
                     /gene="hisC"
                     /locus_tag="PLF14_02835"
     CDS             complement(44767..45819)
                     /gene="hisC"
                     /locus_tag="PLF14_02835"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOT70265.1"
                     /translation="MNNLFSDRLKKLTPYTPGEQLNDKRYIKLNTNENPYPPSDKVLE
                     AIKDKAGETLRLYPDPESKELLSVIAKKYSIDNDMVFVSNGSDETLAFAFNAFFSGKK
                     VAFPRITYSFYPVYCKLYDAKEETPEMNYDYTINKEAVSKCKYPLIIANPNAPTGELL
                     SNEYICSLLEKDKERLILVDEAYIDFANQPSMAEYVKKYKNLLVVRTFSKSYSLAGMR
                     IGYAIGDKALIAALNTVKNCFNSYPLDRLAQISAVTALNNQEYYDMINNKVKATRDRV
                     AKQLDKLGCTVLPSQSNFLFVKFGGYDSSTIYTKFKDEGILIRQWNYIPDYVRISIGT
                     DSDMDSLINTAKEIMK"
     gene            complement(45812..47098)
                     /gene="hisD"
                     /locus_tag="PLF14_02840"
     CDS             complement(45812..47098)
                     /gene="hisD"
                     /locus_tag="PLF14_02840"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOT70266.1"
                     /translation="MIPIFDKDKAGFIAKVRKDGFLDNKEVYDIVYKIMQDIRKDKDK
                     ALMQYTKKFDKTDIAPEDMLVTKEEIKKAYKEIDKDLLKVIQQAVLNVATYSIKQKEK
                     SFLWNKEEGVKIGQKVTPLRSVGIYIPGGTAPLISSVIMNAVPAYVAGVKEIVMCSPP
                     PLDAARIVAANESHVTKIYRAGGAQAIFAMAFGTETIPKVDKITGPGNIYVAYAKKMA
                     YGYCGIDMVAGPSEIMIIADETANPRFVAADMLSQAEHDKLSISSLLTTSKKLAKEVI
                     KEITVQAKELERSDIINESIKNNAAIYIGQDITECIDVLNECAPEHAEIITENAEVYS
                     DYITQAGAVFVGPYSPEPLGDYLAGPNHTLPTAGTARFFSPLGVYDFMKKSSVIQYDK
                     EALERAYKGISLFAQCEGLDAHANAVNIRFKEKKDE"
     gene            complement(47082..47732)
                     /gene="hisG"
                     /locus_tag="PLF14_02845"
     CDS             complement(47082..47732)
                     /gene="hisG"
                     /locus_tag="PLF14_02845"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004053355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOT70267.1"
                     /translation="MEYLKIALPKGRLAVQTVELFKKAGIPCTDIDDDSRKLIHTDQS
                     NKIKIFLVKAADVPVYVEYGAADMGVAGKDTLMEESKNVYDVLNLGFGACRMCVAGEG
                     KYLNRTDKIMNKRVATKYPKIASEYYRNVKNENVEIIKLNGSVELAPLCNLSELIVDI
                     VESGKTLQANGLVVLEKICDISAHLIVNKVSMNMKTDKISYIISALKKELSNDSDI"
     gene            complement(47720..48958)
                     /gene="hisZ"
                     /locus_tag="PLF14_02850"
     CDS             complement(47720..48958)
                     /gene="hisZ"
                     /locus_tag="PLF14_02850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HOT70268.1"
                     /translation="MAYHNPEGMSDLTDSKCFTKKNKEQAVRQLFRNYGFTEIETPCL
                     EYFDMYYGENEYVSQKDIFKLIDKTGNLLVLRPDMTVPACRVAATKIRYEALPYKLSY
                     IAKCFRADEFGGGKQREFTQCGAEIIGSDNPYYDALVIKLAVSTAKRVGINDISIQIG
                     QVEFFNGLAKEASLTQKESEIISKMIDSKDSFGISEFLEGRKIDKDIYNHLTAITGYF
                     GDKSLIDRLLETKLNLRSKQALNNLKQILDILDKMGLSGYVTVDLSMVKKLGYYTGMI
                     FNGNTYQMGFPILAGGRYDNLAEKMGKKLEATGFSLSLEMAIKALERQGQVEELPVMP
                     AIVSFSESMRDYVLSVTKDIEEDKNIQVLPDSDDLERYMKERNISRMARCIAPNHVII
                     RSNGKTEITDFAKWRALWNI"
     gene            49262..49609
                     /locus_tag="PLF14_02855"
     CDS             49262..49609
                     /locus_tag="PLF14_02855"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT70269.1"
                     /translation="MKKLSTAKLAEIMKTRRKKLNLTQEDLSKKTKINRSIISRMEKN
                     NFIPSIIQLENLAKALQFDIETLFYDDTENHTFTALRSKELSAEEDNELKKLYALIFI
                     LQKQIVLSEGIEK"
     gene            49606..50478
                     /locus_tag="PLF14_02860"
     CDS             49606..50478
                     /locus_tag="PLF14_02860"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HOT70270.1"
                     /translation="MNDINTILTEDEIAELQMLASEKRTSLFYSNNVPVANGLPMILD
                     KLNIHLLEYPIKSLNGCNAFSAVILCNRINGRKYTFMGINSANSLTEQCFDIAHELYH
                     FFTDNGIKSCSEQENKRDEAKANRFAAEFLLPETALKRILLDEFNKTSLHDIDIFVLI
                     RLIARIHCNWWLPYVAIVRRLFETQAISQKQSDALLFPDNNDYYKYFERICHALDSDT
                     FLKLNTPTNKIGISPKNIEIIIKNYENDLIDDNTFIKVLNMFNIDPASFGYDFEICDD
                     DYNELEEVLEEVDS"
     gene            50741..51181
                     /locus_tag="PLF14_02865"
     CDS             50741..51181
                     /locus_tag="PLF14_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70271.1"
                     /translation="MKVFNDESLSYNEQILRNTIESEIGNCISYSALYNEKQDRGEIK
                     TLSFMAVKEYKYLVSNDNDCVIIVRNKLPNCSIIFTYELIYLLSEIYPKKAEDLRKLY
                     RFLYYSISKDKQHNPSRSDFKTKMSILYKSSLPILKEISKQRQI"
     gene            complement(51301..51978)
                     /locus_tag="PLF14_02870"
     CDS             complement(51301..51978)
                     /locus_tag="PLF14_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004842391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HOT70272.1"
                     /translation="MDLFLTQSDGNYLLTTYGYIAIALLAVLVFCAIYFFRPKKEKSE
                     LSVNPTKKLAFCSMAVAAGFVASYIRLFSMPLGGSVTLLSMFFICLAGYWYGTGTGLI
                     AAFTYGLLQLVQEPYIINFWQVCFDYLFAFTALGLSGLFKDKKNGLLTGYIVAVLARG
                     FFHTVGGYLFFMDWIPEKFPEKLIPLYPVIYNYSYILAEGAITVALISVLPVKKALER
                     VKAMAVK"
     regulatory      complement(52017..52112)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            52239..53375
                     /locus_tag="PLF14_02875"
     CDS             52239..53375
                     /locus_tag="PLF14_02875"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT70273.1"
                     /translation="MISTRMCTYKDKAAMKKLWTELFGDSKSFTDWFFDNRFSPSDSV
                     CTYDDELLVSCAHSLPMPVLIRGKEIPCAMISGIATVEAYRKQGLMHKTLLYLINELN
                     AKGIYAATLKAVNLSTYYSMHYYGCTLSAYIRSGKKDKYACLMPYIERKANSEVSCRQ
                     IDIYNEFDGLHACYTGFTKGYSGIAIRDKAAFKLKMNDYLSDNAKAIAVFKDGKITGY
                     CIYFDEKDLYAEEYITDDLYSACAMFKALKEQGKSITVKTSKKVADRVGVKNMKVTEQ
                     NSMGIINIGKLLESICGNGDYGVKVFDSFYNPNNGIYLLNGKKVNDKYHLELSSGHLM
                     QLLTGHKSLEELVSEGVAVIYDKVACDEISDMLPEQNCFIADEY"
     gene            complement(53399..54451)
                     /locus_tag="PLF14_02880"
     CDS             complement(53399..54451)
                     /locus_tag="PLF14_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70274.1"
                     /translation="MERIIKKQYKLYPESEMADWFKLFCQSEFGNAHIPEDKNSNFIA
                     LKDEIEDKKSNKTDIEDIGNGNVRVHLGYLKKIGLSQELFTEIMMQSRLLSKGTREGF
                     SDKVKALEKAVKESSLPITEEEFYTFKESYYAAGCRLISHSERYKALYNPSYRVIRAE
                     YANKMKLWAKVEKLQNKARISGTTVNIAIDGKSTAGKSYLGDMFSKIFDCNVIHMDHF
                     FLQSHQRTMERLLQPGGNIDYERFQKEVIPNLKSPTDFTHHAYNCHTNEMSEIKICAK
                     TLNIIEGAYSMHPYFGDIYDLRIFMDIDKETQFKRILKRSGELYKSFTNRWIPSENEY
                     IESFKIKDQCDFIFKA"
     gene            complement(54451..55566)
                     /locus_tag="PLF14_02885"
     CDS             complement(54451..55566)
                     /locus_tag="PLF14_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70275.1"
                     /translation="MDNTTRAMNILNYRDADRMPAVHFGYWKELLQEWVELGHLKPEE
                     IVGVGDGNDADYRLNAKLGWDFNWRTTRRGSASLRPSFKSEVLEELPDGSRKVLTSWG
                     VIEIVKPGIVSISGECDYLLKDRKAYEELFKPKLQYTEDRVDPDYFNNNNWDEIDAPI
                     GLELGSVAGDIRNMLTLMGMSYMIYDDYELLDEIVSDYAELQYKVAERTLQITDFKFD
                     FAHYWEDICYRNGPLFAPDILDNLCAKHYKKRNDLANKYGMKIISLDCDGCIDQLIPM
                     WLENGVNTMFPIEVGVWNASIAPWREQYGKELRGVGGMNKNVLSYDKKAVDDEIERLK
                     PLIALGGYIPCPDHRLPPHTKYELVQYYAEKIKTVKI"
     gene            complement(55683..56414)
                     /locus_tag="PLF14_02890"
     CDS             complement(55683..56414)
                     /locus_tag="PLF14_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOT70276.1"
                     /translation="MIEVKRLHKSFGPKGSLEVLKGIDQTIQKGEKVVIIGPSGSGKS
                     TFLRCLNMLETPTSGEIYYNKQLITDRLYDINKLRMNIGMVFQHFNLFPHLSILKNIT
                     LGPVKLKGKTENQAKEQAMELLQRIGLCDKADAYPSQLSGGQKQRVAIIRALAMEPQV
                     MLFDEPTSSLDPEMVGEVLDLMKKLADAGMTMIVVTHEMGFAKEVGTRILFMDDGLIV
                     EENTPEKLFTHPQNQRTKDFLSKVL"
     gene            complement(56401..57117)
                     /locus_tag="PLF14_02895"
     CDS             complement(56401..57117)
                     /locus_tag="PLF14_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOT70277.1"
                     /translation="MKENFSEFIEQFKISFVNNFITDNRWQYLTKGLLVTLEITFFAV
                     MIGIILGFLMAVVRTTHDKTKKFKVLNAICKGYITVIRGTPVVLQLLIIYYVVFATVA
                     VDKVLAAIVAFGLNSAAYVAEIVRSGIMSVDPGQMEAGRSLGFTHGQTMWYIVLPQAF
                     KNILPALGNEFIVLLKETSVSGYIALADLTKGGEIIRSRTYDAFMPLIAVALIYLGIV
                     MLLTYLVNKLERRLAKSDRG"
     gene            complement(57180..57968)
                     /locus_tag="PLF14_02900"
     CDS             complement(57180..57968)
                     /locus_tag="PLF14_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOT70278.1"
                     /translation="MKKIIATVLTTILALSVLTGCTQVAPNTVFSADDLPGKVIGVQL
                     GTTGDIYASDYEAEGSVIERYNKGADAVQALKQGKVDCVIIDNEPAKAFTAKNKDLMI
                     LPDPFELENYAMCIAKSNSELKDKINAAIAQLKSDGTLAAIISNYIGDDTKGKSPYVS
                     YNTDYSNGKLTMATNAEFEPYEFMEGNTIVGIDVDLTQAVADILKMELVIENMDFDAI
                     IAAVQSGKADIGVAGMTVTEDRLISVDFTDSYTTATQVIIVRKK"
     gene            complement(58214..60817)
                     /locus_tag="PLF14_02905"
     CDS             complement(58214..60817)
                     /locus_tag="PLF14_02905"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOT70279.1"
                     /translation="MNEDELLDSYEEEQEESADLEYVEYTPSFFSTLLSTFWAALKII
                     IAVVMIAAFLIGGLGLGIITAWISTSKVLTEEDLAITTGLTTFIYDIDGNVIETLTGS
                     DNINREIITSKQLPVVLEHAIIAIEDERFPTHSGFDFIRIGSSVLKLIENRGDIAQGG
                     STITQQVYKNVTGRFEQTFERKIQEIYNAILLEMKYDKKQILTMYANLVNMGNGCYGY
                     QSASKMYFNKTLDKINLAEAAVLAAIPNSPYTYNPYGTEENIRKLMYRREDVLDKMLE
                     IGYIDQKEYDDAMAYEIVFAEKVDYVSSSITSYFVDYVITEAIDLLAEKNSISKVQAE
                     RIIYNNGLHIYTTLQPKVQDALDSVFTDNSYFAEYKEDGTIININAVRYGEKPQGAMV
                     IIDQYTGGIAGLYGGNGEKTGSRTYNRATGMNRQPGSTFKPIAVYGPALDLKLITAAT
                     VIDDAPCYLDVKNPDLIYPTNYDSGDYQGLTTIRNAIKASVNVVAAKVWAYYLGRDNS
                     VEYLERVGIDRSEYIYDDSTVSAAIGGLAVGVSPLEMASAFSTFANKGIHMNAHSITR
                     IANNSGETVLRWANTYTVAYSEQTAAIMTDMLEEVVKGVSTTYWINGTAYGSVYINDG
                     QISVAGKTGTTSDYLDKWFCGYTPYYSAAVWYGYDNRVSPVSLVKEEYGRALKIWNTV
                     MNLVHKDLPAKQFTMPSAGIVEREICIYSGKLVGNFCTSDPRGRKTVITEYFIEGTEP
                     KETCDVHQEVVVCAYPDNKDEYGRFYVASDDCPASFVWAMTRLVRPGGAFKKVHEETF
                     SVTVDGKVIWQIERVPYDVVYEVDMTAVCPLHHKHPEEGEENTETEEPEVVEPVGEQP
                     EGTESPTPPVN"
     gene            complement(60883..61242)
                     /locus_tag="PLF14_02910"
     CDS             complement(60883..61242)
                     /locus_tag="PLF14_02910"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOT70280.1"
                     /translation="MIRQKKLDVLIGAMCTIKGDINTKGSVTVEGVIEGDIIADGDIV
                     FAKSAKAKGQFTGANIILSGEAEGILKAKKYIKMTSTANFIGDTYALSLISDEGAKFT
                     GKCYVVESDKEDKTRKE"
     gene            complement(61257..62216)
                     /locus_tag="PLF14_02915"
     CDS             complement(61257..62216)
                     /locus_tag="PLF14_02915"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOT70281.1"
                     /translation="MRKGRTKLEGEGTLVYIPNGGKSSRVLKVTSPILKLSLIFYFMI
                     AVIVCLSLSLVYFINENKRMNAEIDSIMAGYNSEMIALSTYVGKQAMVLDNKLNEIAT
                     LETAQQDLNIQIKELSSQLKLIAEKYTDSITLASASVTANNVAKFAEDTASVAAALEI
                     LLATNESTRTELVDFSEAANVLCNYLDSIPTLWPTKSKHISSQFGMRNHPILNQYKMH
                     TGVDIGGAVGDEIYASATGTVVAVHYEKSGYGYWLRIKHSESISTLYAHCSKILVKAG
                     DTVEKGQLIAYVGKTGLATGPHLHFEIRVNEVPINPTLFISAE"
     gene            complement(62286..63128)
                     /locus_tag="PLF14_02920"
     CDS             complement(62286..63128)
                     /locus_tag="PLF14_02920"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOT70282.1"
                     /translation="MKRYVKAVMFVLVLVLVATFSVSCADTKENLFSINIINGEEIHI
                     TKYHGKSNRISVPAEIKGLPVTGVTSNAFWGNTEAVSIVLPDSVEVIGNNAFFSCKSI
                     TSFNIPKSLKELGDNAFTGCDNLKSITVTVGNEYFISKDGVLFDKDMTKLIIYPARKT
                     GKQYTVPAGITHLGRNAFCNNTYLTEIILPEGLVEIGPTAFKNCSGITKMEIPEGTES
                     IRASAFWDCVNLESVNFPASVNNIGEQVFYGCSKLTVIYVKEGSFAHQWCEDNSRTAG
                     MEFY"
     gene            63335..64756
                     /locus_tag="PLF14_02925"
     CDS             63335..64756
                     /locus_tag="PLF14_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HOT70283.1"
                     /translation="MRNAILIKDCTLKNCETDKALNISFKEKLEIARQLENINTDIIE
                     TGFADTSQGAFIAVKTIASNLKKSILCCPSQAFKASIDKTYEAIKGAVRPRIHISLPV
                     SSVNMEYQLHMKKNQVVETVRQSISYAKTLFSDVEFTALDATRSDRDFLIEIINTAMD
                     AGATTVGVEDSVGHMLPDEMHSFLTDLIKSIGRDNIAFSFRAKNELGMAVANTLSAIK
                     AGVHQITCAINGTANDASLEETVSAIHTRQDAMNVQCNMLIDQLNRASVLLSGITDIK
                     NGKIRGTAGTGLHISESDLLDKEMDFYSFRTFVERLGYTLTEKDIERIYDMFKELCAK
                     KQQVYTMDIEAIIGREAVQVPRIYRLDRFVINSGNNITSTAFIRLMYYEKPLEGVGMG
                     DGPIDAAFHVIEQIMGMNIELFDFNLKSVTEGKDALGAATVKIRYNGKIHVGRGLSTD
                     VIESSILAYINAVNKMLFEEGIQ"
     gene            64753..67266
                     /locus_tag="PLF14_02930"
     CDS             64753..67266
                     /locus_tag="PLF14_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT70284.1"
                     /translation="MKYAFYTANRNNLTWKEAITTAKDLGFSGIEIGAGLLDAQPFTS
                     QYILRTAEQLANLNLEISCFSSSCSLNDREKRKENIQQLRELIDIAARMGASFVRLLP
                     DDDIMPNDRATDQAVISAISAILPYAEKKHVILLIDTIGIYCDTDRLRKVLEHFASDN
                     LAALWNVHHPYRFHNESAEKTVQNLGAYIKYVHMKDSLMNDGAVKFTLMGEGDLPVDE
                     IIRALKSVNYDGYIAYEWITDQDSDLDEADIVFPHFMNYITSKFEENTKANKLYYNKA
                     KTGRFVWKKEQLIDCTFPQLLDRMVEEFPDQYAFRYTTLNYTRTYSQFRDDVDTFARS
                     LIALGVKKGDHVAVWATNIPQWYITFWATTKIGAVLVTVNTAYKIHEAEYLFRQSDTH
                     TIVMIEGHKDSHYAKIVKELCPELETSLPGQPLRIKRLPFLRNIITVDFSMQGCITWE
                     DAMDMHVKTPVEEVYRRANSVGKHDVCNMQYTSGTTGFPKGVMLTHYNIVNNGKSIGD
                     RMDLSTADRMMIQVPMFHCFGMVLAMTACVTHGSTMSPLPYFSPRPALNCINLEKITC
                     FHGVPTMFIAMLEHEDFKKTDFSHMRTGIMAGSPCPIKVMEDVQNKMNMTEITIVYGQ
                     TEASPGCTQSSTDDSIELRVNTVGKALPGMECKIVDPETYEDLPDGTDGEFVVRGYNI
                     MKGYYKMPNATAAAIDKDGWLHTGDLARRNNGYYKITGRLKDMIIRGGENIYPKEIED
                     FIYTHPKVKDVQVIGVPDEGYGEEIMAVVILKDNETMTEKDLKDYVRTHMAKHKTPRY
                     VEFVEEFPMNAAGKILKYKMRENAIEKLKLQKANGIVTA"
     gene            67356..68369
                     /locus_tag="PLF14_02935"
     CDS             67356..68369
                     /locus_tag="PLF14_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOT70285.1"
                     /translation="MKVLIIGATGLLGSEAARELIKRGHSVTGMALPPVPQGADIPDQ
                     MQLILKDCMSLNIDELASIMKQCNGLVFAAGVDERVEGPPPIYDMFYRYNIVPLQKLL
                     PLAKECGIKQTVICGSYFTYFDRTFPEWELYKHHPYIRSRADQAKIALDYADENFAVS
                     ILELPYIFGAQKGRKPVWTFLVEMIRKMPVVTMYPKGGTAMITARQVGQCIASALEIG
                     KGGQLFPVCTYNMTWKEMLAMFHEHMGMPNRKIVTVPTCFFKASVKKLAKKQKAAGHE
                     GGLDMVKFVKVMTYEAYMSDFNAKTILRARPDDINKAIGESVRLCLDILDSNTQVVDM
                     KGE"
     gene            68370..69614
                     /gene="rlmD"
                     /locus_tag="PLF14_02940"
     CDS             68370..69614
                     /gene="rlmD"
                     /locus_tag="PLF14_02940"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOT70286.1"
                     /translation="MKQVELNIIDISYEGKGIARKDNLVYFTDNAIDGETVTAEAYET
                     KPKYVKAKTIQILKPSKYRTEPKCAVYNKCGGCSLRHMSYERQLKLKKDTVIANLKRI
                     AHINIADITIHQNPKTDYYRNKAEYKIKNGKIGFYEDKSHNIVTHEECLLLKEEINNI
                     KKSIEKHITKDMTALTIKEADDGYMVIAESDKTLPDKLINTLKENNKVISVYQRKQNL
                     NIQIYGKQYITDSLCGLKFQISPDSFYQVNKMQTERLYNIAKHYLKAADKNNNLKDKT
                     VLDLYCGIGTISLFIADSVKYVIGVEIVKEAIDNAKSNAKLNAIENCQFIHADVNDAI
                     LQIEYDILIADPPRKGLDDSVIQAIRQKKPENIIYISCNSSTLARDIGQLSDLYSLSE
                     IEAVDMFSHTSHVECVVLMTRK"
BASE COUNT        20217 a        15158 c        12156 g        22697 t
ORIGIN      
        1 ggcggtgacc tcttttaacc agttccagtg cggtctccga tttgcctacc ccgctgtggc
       61 ccgtgataag aacacccaca ccgtatagct ctaaaagctc cccgtgtatg gtcatccttt
      121 cggccagttc cagattgagg taacttatga gcatggccaa aaataccgcc gtaccttcat
      181 aagtccgtaa aaccggaact ctcttcagcc gtgccgactt tataatctca tcatataccg
      241 gctgcgaatt ggatattatt atacagggta tgctttcatg catgtaggca tccagtctct
      301 tctgccgctc ctcacgggaa agagtgccta aaaagttcat ctcggttctt cccaacagct
      361 gcacacgctc cggacagaaa ccttccagat agcctgcaag ctgcaatccc ggtctgtata
      421 cttccgcaga atctataatc ttcttgtcga tattctccgg aacatacatc ttatccagac
      481 aatacctctc tataatggtg ttcacactga tgctgtgagg agtcgcatca taattttccg
      541 gtatattcat aaagtacctc ctgtaacaat ccgttgcttg ccaatatatc acttttatat
      601 ctcatggaag gcgggttacc tttccagtcg gtacatacgc cttttgcttc ggtcagtata
      661 catatacccg cagccacatc ccacggactt aaattgcgtg taagaaaggc atcggccgcc
      721 ccttcggcca cataacagat atccaatgcg gccgaaccgc ttatacgtat atccatgcat
      781 gccaggaaca ctttcttcag cacatcgaaa tgcatatcgg catcgcctct ttcgtacgga
      841 ctggtctcac atgttataag ggcgttctct atattcttaa catttgaaac ccttatgggt
      901 ttaccgttaa gttgggcgcc cttacccttt tcggctatat aggtggcatc ggcaaccgga
      961 tcgtgcacta taccgaaaac cggctgctct ttatacacat aagccagcga tatgcaggaa
     1021 ggcgtatagc cgtgtaaaaa attggtggtg ccgtccaggg ggtctaaaat aaagacggaa
     1081 tcggataaaa tatcccggtt atccttctct tcggcgataa gcggtatgtc gggataatgt
     1141 tgtcgtaaag cttgggctat acttttttgt atggctatat cgcatgccgt cacatagtcc
     1201 ttatcgcctt tttttacggc atttgtacga taagttgcgt ctttcatcaa tgctcccgca
     1261 tccagcaccg cattaacagc ttttataaaa tccattattg tcctcttata taaaccacac
     1321 cgtctattac ggcggcagct ccgtcacgcg agtaggaaaa agtatcttcc ttaccgtcat
     1381 atttaaatgt aatggtcttg ttttttataa cataggtccc gcttatttca ttatcatttt
     1441 ccgtacatgt aaactcatta ttcataaact catattttct tccggtctcc tcacttacat
     1501 acacaccgaa aagatatgtc tggcatgcgg taagagtgag taatataacg gctgttaaaa
     1561 ataagataag aatctttttc atatatttat atacctcttc tttcttccca aattatatca
     1621 tgtatcacac atacactcca cctgtgacgg attatcggta agattctcca cattaggcat
     1681 atatatgtca tgtgtaacga gatttcctat ggcaccatat ctttcacagt ttttaaattt
     1741 cctgaaagtt atgaaggcat cggtctttac ggcaccctca tcggaagata ctatcacgtt
     1801 ctttaaaaac agattttcca cacggtcata tacatgtata tatccgtcaa ccttcacatc
     1861 ggaagcggcc tgaattacag ttaacccgtc aattacgacg ttcctgatac gcggcctgca
     1921 atcgggggcg taatttaagt cgttctcatg gaaaggcaat ccgaacaccc acagcggccg
     1981 gttatccgaa gggttctgat ggtgaatgtt tttaaatgta atacagtcga agttcccccc
     2041 tatatcaaac aaagtggcgg ttctgtaagg atatacatgg tccatgggac gtaagtctat
     2101 attctcaaag gtaatatgtc cgtaattgcc gaaaccgtct cccggaaacc aggggtttat
     2161 gaagaagccg tagcttctgt aagtacctgt tacatttctt accgttaccc tgtccaagtg
     2221 tccttttgat ctgcacagca ttcttatggc ctgatcggca tagtccaaat gcactccgtc
     2281 tataagcaca tcggttatgg aagataccag gtcatgctcg tcgggaccta aggctatgaa
     2341 gtcgtctccc gaccgccctt ttatattcct cattgtaaga aactgccccg gcccccagaa
     2401 atgaaaaccg tcctgattct gtcccctcat ccagttgggc aaatcaatat atatgttttc
     2461 tatggtgaca tttttaaagt tgacgcacat taccgaccag gctctctgat tccttatggt
     2521 tatatccctc atgataaggt cctgcacccc gaaaaactcg aaagccacca ccattctgta
     2581 agcaccgaaa tcgtcggata tgccttctat accgaaacgg tatttctctt ccgttatggg
     2641 tacatggtgc tgttgccccc tgttgttatg gttatatgtt ccgcctatga gagaaacatt
     2701 tctcgtaagc aattttgtat agcttcgctc cttcaaatcg ataagtgaac ggtcggagcc
     2761 gtctttcaaa aagaatccgc agtccttact taaacattca atagttgtat tggatttcag
     2821 atccagaccg gtaataagag cggcgccgtc cattataagg tgaataccct gatcctcccc
     2881 cgctttatcc aatacccgct gtaaagcggc cgttacgtcg gttcctccgc cgttatatac
     2941 attactgtcc aatgtagcca cagagcttgc aaatatttta gtaaacttca ttttgtccct
     3001 cctgtccgat acctttattt taacatctgt aaccgatatt acaattaaaa ggttagggat
     3061 agatatggcg tcagaaaccg tcggcatatt cttttaagaa gtcagccgca ttgagtattt
     3121 tccgatcttt cggaaaatgc ttaaggttgc ctgttatcaa cacggcacca cagtgctttg
     3181 caacctcata gaacatttta tcggcttcat cggtaaaatc aatatccgac ggtttagcaa
     3241 ccaccgagtg tccgttactc tcaaaccaac ccagcagtga attaatttcg ccctcggaaa
     3301 aaccaaatcc cggtcttttc aatacctcgc gatactcggt gattatccta taatcaaaac
     3361 aaggaacaag aacacctctc aaaatcatgc ttacaatctt ggccggattg ctgtttcttg
     3421 accacagtgc cgataccaat atatttgtgt ctattacaac aagcatgaac tactcccccc
     3481 ggcgggcagc cgcaatctcc gcttctatct cctcatcgct catgtagcct tttgccgccg
     3541 ctttagctct catggaatta aaggcgattg cagcctttgc ctgacgaacc gctttcaatg
     3601 tttcttcaaa actgccatcg gagatatcca gcataattgc cgtcggtctg ccgttgttgg
     3661 tgataacaac ctcgccgttg gtaacaaggt cttcccaaag gcttttaggt gtggttctta
     3721 aatctcgtac agtataaaaa ttcataacag tacctccgct catattgtac cgttaatgta
     3781 gcacaattgt caacatattg tgtattttga aaatatttcc gggagtatac atgaatatag
     3841 ggtatacttt tatagaggtg gtgatgatgc ttaagagtat tacgccgagt acaaaggaaa
     3901 attgcattga agaaacaaga gaaaataacc tgtcggaaaa acttgattcc tttcttgcta
     3961 aaacggatta tgtaaacagc ggaggaagtt ttacccgaga cgaaatgaat gaaagataag
     4021 ctttcattaa tacgatatat tcaaggagca atgtctgctt gagaagattg caaaaatatg
     4081 taagagaaat tgtatttaag acttaaagca atattaccga aaaaaacaaa tagattcatc
     4141 gcctaatgtg ggaaatgtaa aggttttaat gtgggttttg ttaatgtgtt tttttgcgat
     4201 ctatcattat ttcatctgtc tttactacat atgcatatgc ctcgggataa gctcttttca
     4261 agtagcaaag aggtaacaaa atttcatgtg taactgtctt atatgctctt ctttaaaact
     4321 atccggttta atcataattt ctatctctcc atttaaagct ttatatcgtt ctctcttata
     4381 aactccgata ttttcttgtc gacttttctc ctattggcat aacggaaaag tgctttctta
     4441 tcgataacat atcgtgtgaa agcatcttta tatattgcgg gatactcact ttcgctgaca
     4501 gattccataa tcaacggttc cgcaaacaga tctaccagta ttttttccaa tcttgcatgc
     4561 caaacaatct cttttccctt aggggcttcc gtcgtaagct ttacaataac aatcatattg
     4621 tcgctccagt attgatgata taactctaca gtaggattta ttaaaacttt accgggataa
     4681 tattctttca gcgtgtcaaa tacaaatccc gtaatatccg cttctaccga cagataaatt
     4741 acattgtgag caatttggtg attaacaaaa tcattcagtt gcaccagctc gaaaacggaa
     4801 taagtgaggt atggatattg ttcagtcatt atatttgcaa cctcctttgc aagttcggaa
     4861 taggtatgag tgtaatccgc agtcctaccg tccggaacga aatacacatc tctgctttct
     4921 cttataatat tgccgttatg tactaatcct tctaatgttt tgtaaaaaga cgcatcactc
     4981 agcgaataac cttctcgtcg aaatgattca agcatctcct ttcttgagaa tttctctttg
     5041 cctttcagtc cgttaagata tttggaatca gccatacacc ataccttcct taatcttatt
     5101 tttgcggcac taatactcgt ttagtgccgt aaagataatt atagtataca tcggaatttc
     5161 aaaatagtct ataagttata agtattaata tgctttttat gacaacaacc ttatactcaa
     5221 taaacttatt gccatcatat gcagaaaacg atttaattaa aacaaggcat taccttgacg
     5281 ataatgccct gtgttttaag ttatttaact ttttcggtta cattccgtat ccgcccatgc
     5341 ccgggtttcc tgccggcatc gggggctctt tttccggtat gtcacaaacc agtgattcgg
     5401 ttgtgagtat cagagccgct accgaagctg cattctgcaa cgcggttctt gcaacctttg
     5461 taggatctac tattcccact tctatcatgt cttcatattt ttccgaaaga acattgtatc
     5521 cgacaccttt cttggaagac attactttag ccagtataac cgaaccttcc agtcccgcat
     5581 tgatgactat ctgtcttaaa ggctcttcta aagctcttgc cactatctga gcaccggtct
     5641 tttcgtctcc ggtaagtgaa cgtatgacgg catttacttt aggcagtgca tctacaaatg
     5701 ccgctccgcc gcccgctatt attccttctt ccacggccgc tcttgttgct gcaagtgcgt
     5761 cttctattct taatttcttt tccttcattt caatctcggt tgcggcacct acctggatta
     5821 ccgctacacc gcctgccagt tttgcaagtc tctcctgcaa cttttctctg tcaaactccg
     5881 atgtggtttc ttcaatctgt gattttatcg aagccactct tgctttaatc ttggctgcat
     5941 ctccggcacc gtccactatt atcgtgttct ctttctgaac tttaacctgt cttgctcttc
     6001 ctaacatatc catggtggtt tctttaaggt ccaagcccag ctcttccgaa actacctgcc
     6061 cgcctgtaag aatagctata tcttccagca tggcttttct tctgtcaccg aatcccggag
     6121 cttttacggc tacacaggta aaggttcctc ttaacttatt tacaactaat gttgccagcg
     6181 cttcgccttc cacgtcttcg gctatgataa ggagcttctt gcctgtctga actatttgtt
     6241 ccagtaaagg cagtatttcc tgaatattgc ttatcttctt gtcggtaagc agtatatatg
     6301 catcgtccag aaccgcttcc atcttatcgg tatcggttac catgtatgcc gagaggtatc
     6361 ctctgtcaaa ctgcattcct tccacaactt cgaggttggt tcccattgtc ttggattctt
     6421 ctacggtgat aacaccgtcg tttcctactt tttccattgc tgcggaaacc agttttccta
     6481 tttcgccgtc atttgcagat acagccgcta ctctctcgat atcgcttttg cctttgatgt
     6541 ggctgctgtt ctccactatt cccgaaaccg cggcatctac cgctttttca ataccttttt
     6601 tcagtatcat gggatttgcg cccgctgcaa ggttcttgag cccttctctt actattgcct
     6661 gtgcaagcaa tgttgcagtg gtggttccgt cacctgccac atcgttggtc tttgtggcaa
     6721 cttccttaac cagctgtgca cccatatttt caaaagcatc ctccagctct atttccttgg
     6781 ctattgtaac gccgtcattg gttatgagag gagctccgta tttcttatcc agaacaacat
     6841 ttcttccctt gggtcccaat gttatcttaa ccgtatccgc cagcttgttt atgccggttt
     6901 ccaacgcctg tcttgcatct attccgtact taatctgttt tgccatatca tatcctcctt
     6961 aaattaaaca acctttgcca atacatccga ttgtctcaat attgtgtact ccacaccgtc
     7021 aagctttact tccgttccgg catatttgct tataagcacc ttatcgccga caacaagttc
     7081 catctccacc ttatttccat ccacaaatcc gccgggacct actgcgataa cttccgctac
     7141 ctggggtttc tccttggccg atccgggaag tacaataccg ctcttggttg tttcttccga
     7201 ttccaacatc cttattacta ttctgtcaca taatggtttg attaccataa ttctacctcc
     7261 gattgtccat attttagcac tcaagacgtt agagtgctaa agatattgta gtttacatag
     7321 cacatattct caaaccgcaa aatcggtcaa atatgctgtt tatccgtaaa tatatcccga
     7381 ttgctttaaa ttactcaaat atgccggtct ttactcaagt ttacttcttt ttgccttttt
     7441 tatgcactgc atgctttata aacagcgtaa acagcgggtg cgctctgttg ggacggcttt
     7501 tgaattccgg atggaactgt acacctaaga aaaagtcgtt cttcggcaac tctatggctt
     7561 ccaccaatat atcgtccggt gatgttccgg agattaccat tcccgcagct tcaaattgtt
     7621 ttctgtattc gttatttatc tcatagcggt gtctgtgacg ttcggaaact tcatcggtct
     7681 tgtatgcttg gtgcataaga gttccgtctt ttatcttaca gggataggcg cccaatctca
     7741 tggtgcctcc ctttaccata cttacatact gatcgggcat gatatccacc actttgtgat
     7801 gacttctttc gtcaaactct ctggagttgg catcggtcaa tccgcatacg ttacgtgcaa
     7861 attccactac cgctatctgc atacctaagc atattccgaa atacggcaga ttattgactc
     7921 tggcatagtt gcatgcgata attttacctt ctactcctct gatcccgaat cctccgggaa
     7981 taataatacc gtggcagtcg gcaaaaacct cctgtgcatt ttcagcggtg acttcttcgc
     8041 tgtccaccca ttttataaca ggcttgacgt tattctccaa tcccgcatgc ttcagagctt
     8101 ccactaccga aagataggca tcatgaagct ggatatattt gcccaccaga gcaatgacca
     8161 cttcgtcttc tattttcttc agcctttcaa tcatagccgt ccattcggtc atatccgggt
     8221 cttttgttac cagacccaat tccctgcata ccaaagaaga gaagtccagt ttttccagct
     8281 ccagaggaac ttcatacaaa acatccatat ccagattttc tattacacag tcttctttta
     8341 cattacagaa aagcgatatc tttttcttta aagaatcatc cagatgatcg ctacagcgaa
     8401 gcacaatcat atccggagat attcccatgg aatgcaactc ttttaccgag tgctgagtgg
     8461 gtttggattt gtattctccg gaactggcta tatacggaac aagcgttaca tgaataaaaa
     8521 ggcagttacc gggacctaat tccaaagata tctgccttat cgcttccaat atatatgtac
     8581 tttctatatc acctatggtt ccgcctattt ccgttattac cacattggcg ttggtctttt
     8641 tacctacgcg gtatatgaag ctttttattt cgttggttat atgagggatt atctgaaccg
     8701 tttctcccaa gtattcccct tttctttctt tgttgagtac gttccagaaa atctttcccg
     8761 aggtgagatt ggagtatttg ttgagatttt catcgataaa tcgctcgtaa tgacctaaat
     8821 ccaaatcggt ttccgctccg tcatcggtca cgaatacttc gccgtgctga taggggctca
     8881 ttgttcccgg atcgacattg atgtacggat caatcttctg tgctgctacc ttcagtcctc
     8941 tggctttcaa cagtcttcct aaggatgccg ccgttatgcc tttacccaag cccgacacaa
     9001 caccgcctgt aacgaatatg aattttgttg tactctccac tatttccccc tttaatatcg
     9061 gatatgtttc catatccgac caatacatat caatcgcata taatgccgat gaataaaacc
     9121 tgctattcat actatcacta atcggactta taatccagtg gttttaagga attaacgata
     9181 tgttttcata aatttttcaa tcctcagcaa tccgcctatc catatgacaa ttaaatggta
     9241 aaaaatgtgt aaataaattc ctgcaaccta catccatgaa aaaggagccg gaagttccct
     9301 tctcgattag cattttttga ttacctttct cgatactata ctctttgctg atttctttaa
     9361 acggttgctc attcaattgt ttaacggaag gctcaaaaac gctggaatgt tatatcttaa
     9421 agtgtataat aagggcagca gcggtattac cgggaggttt tcatggcgta ttttcttaaa
     9481 caaactaaac aaaaagggcg tacttatctt tcaatagttg aaagttttta tcacccgaaa
     9541 aagaaaggta cggctcatag agtatacaaa tctttacaat ctgttgaaac atggattgaa
     9601 aaaggcattg aaaatcctgt tgcacacttc caaaaagaag tggatgagtt gaataaagtt
     9661 aagcctaagg aaaaagagaa aaagatttcc gatataccgc ctgttgttca tctcggatat
     9721 tttcccttaa aaagcattat ggaaaaactt gaaatagaga agaatggcga ctatctcaaa
     9781 ctgacaaaaa acttggactt taatttgtat caccttatat cttctttaac atatgcaaga
     9841 gttgtggaac cctgtgatgc aaatgaagca ttctatgata tactgcctct ccttgatttt
     9901 tcttattgtt tttctcatga gcaaatgctg gaaggattgg ccttcttagg acaaaactac
     9961 gaaaagttcg ctaagatatt taccttacag actaaagaaa aattcggcat agatacctcc
    10021 gctacatact tcgattacga caacttttac tttaatatga cggaagaaag gaatttgggg
    10081 gatggtacag gcaaaaaagg ttctttattt cgttacgcta ttcttacgga taagaataat
    10141 attcctatag gattgaattt tctttgggga gatgtttcaa aaaagtattt gctgaaagac
    10201 attatacaaa gactgaatgc aacaaacatt cctgttaaaa aaacggtatt tgttatagaa
    10261 gaagaagagg gaagtatctc ttttgatttt aattgcggat atctgtattc cccgccttta
    10321 ccgctttccg aaaagacgga aaaagaacgg attctgtcaa ataaggggta cagcgaagta
    10381 aaagacagtt acggcaacct gctttacaga cacaaatcat atatagaccg attcccttat
    10441 acagtaagta taaagggtaa agaagacaat gtaatgcttg aggaaaaaag attaatcaca
    10501 tatgccccct accttgtaga agaagacaaa cttataataa aggctctggc cgagtgtacc
    10561 gatatttact cctttaaaga agaaacggat gaagcattat cctttgcagg atacagactt
    10621 tttattacct ccgatgtaaa catgtctgat cggaaaatat atgacaccta ttattatttc
    10681 cgacatatag tggatacttt cagaagtatg gaatccgata taaacggata ctctccgacg
    10741 ctggaaacgg aagattgtat aaaaggttat ttcttaattt gttacatagc cttgttgctg
    10801 gaaaggctat tctgttttaa ggtattggaa gataagtatt ccgtatccga actatcctat
    10861 tttatggaaa atttcaagct tgtgcgtacc gaaaccaagt atttgaatat ggcaagatac
    10921 acagctttct tagatagact gtcgaatata ttaaaacttc cgcttaccga ttattacctt
    10981 acggaaagac aggtaaaacg tatacgggat tatacattgt gagcattaag tttaattata
    11041 attagttgac tttcagcttg ttaaattgct cctttaactt ttccggaacc tttttctcat
    11101 caactctctt ttcttcccac gattttttca gtaattctct aatatctccg gcagtataac
    11161 gaatgatgta tgtttcagca tcaggatccg cttcgacaac tgtttgatat acccttgtac
    11221 agtttataag tttttcaatt gtaatattca caatatcgta atgtgtatct tgaagatcaa
    11281 caattgcaat ctcctgtccg gcatatgttt tcttatactc aagtgagttt tttgaaagaa
    11341 tatccgtgtc tattattata aaatccattt tggtaatgtc agaggatgta aaagcgatag
    11401 caagtattgc atcttcaata tcatcaagtg aatttatatg ccatgtagac agtgttccgt
    11461 tctttgttcg aaattctgtt gtaggtatat cggcataaat ctctttaatt tcagttcttt
    11521 cgcaaagaga aaacagctcg ctcattttaa ttagctttcg caccaaaaac gccattattc
    11581 cgtcccgtct ctttcaatat gcataataac cctatcagta tacttctgaa gccatttggg
    11641 ggtacaatgt atatttttca aaaaaatcaa accttttttt gaattccatt tttcaaaaca
    11701 ctgaattgct ttatcgcgca cggataactc tttattctcc aaaaggcccg ccgctataat
    11761 ctgacctttt ggataaacaa tctcataggg tacactcgaa atcattgtca gtataccctc
    11821 aagcatatgt ggctcattta aactggaagt ataaatctcc atcaacgcat ctgtcacata
    11881 atcaatttgt cctttatcgt acgcatcatc catgtagagt tcggattggg aaaattctcc
    11941 gtcaatgaac tcatcctgtc gaataagtat ttttatctca tttatatagc gcgatataat
    12001 gttcttcctt actttgcttt cgccaaaaat ctccttcccg aaagtagcgg atgaatccgt
    12061 ctttgacact acagaccgga tagttgattg aaatcgcgaa gagttcggaa aatacaatat
    12121 atttgtttcg cctactgtag cgacattttt gtaatctttc gtgctattct ccatttgtaa
    12181 caacctttca ttatcgacta ttaattccga agcaataggc atcactttcc gaataaaact
    12241 ttctattgat tttttcttaa aacgcatatc ttgattttgt tgtaaggtat ttatatcaat
    12301 atgaaaaaca acacctgctt tttgctcttt tacatgttgt ccgtaagata tatcggtaat
    12361 tacattaaga gtttcagaaa atccgttaat tagaatatcg gtatgagcat tcgttcgcat
    12421 agcccattcg acaaaatctt tatctttgta gtatgcggca ctcttaagta gagattttcc
    12481 gcatttttcc aacttatgga cattctccat ttcatgtacc tgcaaaatat tcatagctaa
    12541 tctgttagaa gatatagaaa agtagtcaat tataacggta aatacttttg tcgctaatat
    12601 gaaaaaatct ttcatatcaa tatccgactc atccaaatta ctgtaattaa tatcaattct
    12661 atcgtcaaga attgtaatac tatatcgttg atcctgagag ataaaaccta tgttatatcg
    12721 gacagcattt ttacttgaag gttctgaaat cggctgactg ttaacaacaa taggtaacaa
    12781 aacttgctgt atctcttgat tcattttact cataaaatcg ataattattt ccggtgaagg
    12841 ctcaattgcg gagtattcac cgaatattga taattggtaa ttgagtggaa caaacataaa
    12901 aatcctccat gttgcacata tgtgaagtat agcatttatc gtctcttcaa tttctaactg
    12961 tttgctttct taatgactca cttttcttct ttacaaaact ctgcttattg ccacagataa
    13021 ctatactacg aaagcattat aaacaaagaa caaagagaaa gcaatatact tatgtgtgtt
    13081 tttgacattt attgctatat ttgccaagca gatataccgc ttaaataccg gaacagaact
    13141 ctgaatgata taaaatttta agaagaaatc tttttacaat acttctccaa cgggcaacct
    13201 gcacattccg ctttcttttt acagtgctct ttaccgttat gcacaataag tgcatggaaa
    13261 cggttatata tttcaatatc cgaaggaagt tctttttcac agtaattctt gacttccata
    13321 tatgtgtttc cggcatcaac agggaaacgt ctgaaaaacc tcattgtata ggcatctacg
    13381 acaaaagaag gtaaacataa tgcatagagc aaaatggagt ctgcggtttc attaccgaca
    13441 cctcgtactt tcaaaagctc agcacgaata tccgataatg aacattctct aatcaaatca
    13501 atattacaat cataacccat aaaccattcc gtaacggctt tcagatattg tgccttttgc
    13561 ctgaaaaaac ctgcaggacg tattatttcc tgcaactctt ccatagataa agacataacc
    13621 ctttcaggcg acaaatcacc ttcaaatcgt tcaattgctt tttccacatt cgaccaagcc
    13681 gtattctgtg taagaattgt accgacaatc acctcaaaag gtgtgtctgc aggccaccag
    13741 ttaaggttgc cgtagtgaga gtataaagtg ttatagattt tttttaattt attcataaac
    13801 cgatgccttt atcttgttta tagccctaat atatttctgt tactctacca atcaatagga
    13861 actcaactgt aaatcactta cctctctctt gatacacctt tataatctct tctaacagtt
    13921 tttctatttc tttatgagct ttataatact tatcaggaaa cgagttgtta ccgtccgctc
    13981 ttattttctc cccgttctcg aaatcaacac tcatattgaa catggtaccg tccttaacaa
    14041 aacgattgtt tccgtcaaac ccgtcccata aatatatctg atttccgtaa tatatcctta
    14101 aaacccaata atagaatctt tttacaatat cctgatcttg ctctttatcc cagactactt
    14161 ccgcttcctt atacgagttt tcaggatcgg agaaatttgc cttaaaaagg tatttgtctc
    14221 cttttttttc tattgtaaag ctgtaaatct ggggcattgc catgcctccg tgtctaaaat
    14281 aaaaacgtgt catttcggag tctgaaagaa aatctttcct ttcacctttt ttaccgaatt
    14341 cggttgaaaa gagttcatat atttccttca tccacgcact tttagaatca ttatctacac
    14401 agaaacgtat tgattcacag gacctgtacc tacaaagaaa tcttgtagga tcgttaggaa
    14461 gtcccgatgt caccctgtat ataccgtttt tgcttgccaa gttatattta tctattatct
    14521 tctgcaattc aaccaaaaac tccggtcccg tatgtattgt tttctccatt ttaacaagac
    14581 cgcctgtcag ttccgttacc gttctgttaa ttctttttgc tttaaagtga taaaacaatt
    14641 ccccgcactc attggaatat acaaattcgg catcaaattt actgatcttc tccgacttta
    14701 ttgtcttagg agcattcttt tttgttttat cggtaacctc tcccggcatt tttatcatgt
    14761 ttctgaaatg cagatacaaa acaccgccta taacggcaag tactactatt actattatta
    14821 tgataaatat cggcttcatg gcattctcct atctcaatca gtaagtaagt tcatttatat
    14881 tattccaaat cacttactct tctcgtgata tatcttaata atttcatcca tcagtttttc
    14941 tatttcatcc ttaacttcgt tatatcccgt tggaaatgaa ttgcttccac aagcactgat
    15001 gcttttcccg ttcgcaaaaa taatactcat atcaaactcg gtgccgtcta aaatatcgtt
    15061 attacataca tcaaagccgt tccacatgta tatatgattc tcgctgtata tccgtataac
    15121 ctgattatat atcccttcta caacatcgct gtattccttt gtatcccaaa ccggctttac
    15181 ctcattatat gaattctccg gatcaaagaa atccgctctg agacgataat cctctcccct
    15241 ctttcttata tcaaggctat atatataatc ttttcccata ccgctgtggc tgaaatagaa
    15301 gtcggtcatt tccgattccg aaagaaaatc cttcctttct cctataccgc caaactgaat
    15361 tgcaaacaat tcatatatgt ctttcatcca ctcactgtaa gaatcaccgt caatatagaa
    15421 gtagattgat tcacctgatt catatttaca aagaaagctc gtaggttcaa caggtagtcc
    15481 cgctgtcact ctgtatacac cgttatcgct tgcaagttta tgtttatcta ttatctcctg
    15541 cagacttact aaaaactcac accctacatg tgttacatcc tcaatgtcgc cgggacctaa
    15601 agtaagttcc acttcggtat tacttttctt aacggcacta aattgataaa acctttctcc
    15661 atattcatca gcatagataa aatatgtatc gaaactttta atgtcctttg actttataac
    15721 cttaggagca ttcttttttg ttctgtccct gacctgaccg ggcattctct cttggtccct
    15781 gaaatgaaag aacaagacag tacagacaac aataagtatt atcaccactg ctattatgat
    15841 tattatgtat aacggtttca ttgtgagtcc tttcctttct gttgtatatt atagaatatt
    15901 ttaaatgcct tctacactat tatatatccg gaatccttat ttattaacgt taatcaaaat
    15961 gaattgtctc ctccgccctg ttatatattt caattctgtt ttcaatattg actctattaa
    16021 ccagccaacc tctgaactga cattgctgta taaattcctg tattcggttt acaccgttta
    16081 tctcctttaa tgtaaccact aaaccgaatt taaggttttc tccgtcttcg gtattcaatc
    16141 tttcttttgt ttttatgctt attccccaca gtccgttttc aagtttttct ttcggttttt
    16201 tttgccttcc tgtaggagtt tttacattct ctctgatatg ctttacatta tcccattttc
    16261 tgaataattc acgcacagtc ccttcataca acaataaatt ctcttcatcg gactgtttgt
    16321 tatcatttat tgaatcaatc tttatttttc cctttttagt tttaataagt cttccgaaat
    16381 gaatatccat ttccgtattg gtataatcta caccttgatt tcttgaacaa ttgggaaaat
    16441 agcaaagggt tgccttagat acaaacggtt gcttacctct atcttcggga acgggaatat
    16501 tgtaattata tgtatcgtac ttttccgata ttccgtctat cgtaaacttt atttcatcac
    16561 cgggtgtcat aacaatatca tcaatatgta tcggaactat accgtaacct atcaatgtgg
    16621 aactgtacgg cttactgtcc caccgcgcag cggaatctat tatcagagct tttgccagtt
    16681 ctcttgtaaa gcctaatact tcaataagga atgcaacttt tcttgtaatc cacggtgccg
    16741 caaatgaagt accgtaaacc gtttttgcac cgtaaggtga acatactctt attccttttt
    16801 cacctgctcc gccataatag ctgacatcgg gtttattaaa aaatgaaaga accagccctt
    16861 cccttgagta ttctgcaggc tcatcatcaa aactcaccga attaacaact aatgagttaa
    16921 tagaatctgc cggtgcaccg attttcttta gattggaatc tttaggtttg ttagtacctg
    16981 ccacaacaaa tattacatca ttctcatatt gtattttatc cagtatagcc gcttcgggag
    17041 aaataaaatt ggcatttatc tccatagccg atcccaacga caaattccaa actttaatat
    17101 ctctgttagt tgctacaatt tccttaatgg ctctgagaat agtaaaggaa ctgaattgtc
    17161 cgccttttga cacgccgaaa tgtctaacct tgaatctgcc gcacccgtcg tccaggtccg
    17221 ggttaatacc ggctccgtca acaataatag acgagaccat tgttccgtgg tcataatctt
    17281 taccttctaa cggaatatta ctgtccagca tgtttttaaa ctccacccat tccgagaaat
    17341 agacattctc atcaaacatg gtatctatga cacctattgt aggctcatta ccgggatgcg
    17401 gtatcagagt tgatgtttca taggtactag acatatctac tttttccagt tttgaaatat
    17461 ccgttacagc cattgatatt aaatatggag ccttttcttt caataacctg tattgatcgg
    17521 gtttaagcaa aattgttgtc tcgtctataa tcctcacatc caataaatct atacccacct
    17581 gtttcagaat atcggaagtt tttgttcccg tatcatatat tgttataatt gcatcatcct
    17641 gtaaatcgga ggtatcacgt tcaataccga aattttctat gtagtatgca tcaactattg
    17701 tatttactaa ccttgtcttt gaaagacctg cagcatctct gcttctgaat atgcttttat
    17761 acttatcacg attaatatca tcaatcttat catatgttat ttcttcaccg aatgtctcct
    17821 caactatttt tataactctc tttaaattat taattgcatc tataagagtt ttatcactta
    17881 cgcagtgggt tattatatgt ttcggggaac cgtcaaaaga aaattttgca ccgacaattg
    17941 attggttatt cttcctgctt ccgctttcca aaattccctt cattctgttg ctttttgcta
    18001 caacacatgt ataatacaca ctgacaagcg gctttattct caaactttct ttttcccaaa
    18061 attcgcggac tttaactaaa tcatttttta agtcaattaa acgtgaaact ttcacttctt
    18121 tattcttggg tatatttgaa tgtcccggtc tgttaccgga atctttttgt tcaaatcttc
    18181 cctttaattg aagtatctca ttcataagta agttactcct ttaattctct tgatacctga
    18241 ctcttggaaa ccccggtcaa tatttcaatt tctctgacag taaaaccctt gtcctgtaag
    18301 acttttaatt ccaatccgcc ttcttcgcaa accgatgcat acagtcttct taaataatca
    18361 aattcattat cgggactgct gaatgccagc gatgttttta taatattctt taaatctccg
    18421 ggataaggta tcttctccat tgaagatata atcttcttga ataggcgtat gtttcttccc
    18481 gcattatcaa attttgataa gtaatgattt aatattattt ccgctatatc aatcagatct
    18541 tctcgtgagt atctgttaaa atctattaca aaatcaaacc ttcgtattaa agccttatca
    18601 aaatattcaa ataaatttgt tgttgctata agtaaaatcc tttcatttaa ttcgtctaaa
    18661 ccttttaata cggaagtcat agctcttccc atctctctaa gatcttttga atcggttctg
    18721 tctaatgcca aagcatctat ttcatcaaac agaatcacaa ccttttcagg ggctaccaag
    18781 ttatttatct catcaaacag ttttgctata ttttttccgg tctgaccaag tttgctgtct
    18841 attacataag caaaatctac aataaacaaa tctctattca atattctcgc tatatgtttt
    18901 accgtttccg tttttcccgt gccgggagca ccttggaaca gaaacttatt aacacctatt
    18961 ctatgtccta cggcatttat aataccttta acatcctcca gaatcttttc aggaagaggt
    19021 aacggatctc cggtaacttc tatttttctg acaaatgtta aatcatcctc atttatctga
    19081 ggaacaaagg tattcgcatc ggatagaagt gccattatat attcggaaag ctggtaatca
    19141 cccatcttat caaatatatc cgcaatctga taagcttcat tcctgaaacc tacatcatta
    19201 ttctctgcat agtattttat taagttaata atattagcct ttttcatatc ccaacacccc
    19261 ctaccgttta cttccgaaga cattatatca cactgggaca ataatgcaat agttgggaca
    19321 ggaatgagat atttttaaat ttggaaacaa tccacagaat aagattgcta aactcaagaa
    19381 acaaagccaa gtaactctgt cagttcggaa gggtggctct catcctaccg gactggcagc
    19441 tccgtcgcaa cggaataggc aatgaaagaa catgacagta cagacaacaa taagtattat
    19501 caccactgct attatgatta ttatgtataa cggtttcatt gtgagtcctt tcctaacctc
    19561 aaagaacggt caattatacg gtttcgattg tattttgcga gaactcaaga cattacattt
    19621 cgacttattt gatttatgta acatcttttg gcacttttga ctcttttatt ctgggcacat
    19681 atcgattgca tttttcgcca atatttgcac acttttctaa ctgaactgat tcaatattag
    19741 ttttcgaaat agtttgcaaa gtttcattaa tgttatttaa atttataatt agttccttta
    19801 tctcacttct caactcatct atttctgata tatattctaa aaatatacta tagtcatcaa
    19861 aacatattat ttccttttca gtaaattttt tttgctttcc ttttgaaacg tatgtatatg
    19921 tgacttcaag catcttattc ttgtagtcta caacttttga aaaagtctct ccaagacata
    19981 aaacccattg ttgctttgga tgaatagata catttatttc cttaccaact aaacaacgtt
    20041 tctgagcatc gtgcgaaagt tgctttataa aactttcatt atactgaacc ttttttaata
    20101 cagcaggtac tacaccaaca ttccgtataa ctatacacac taaagtacct ctaatcaatt
    20161 caagggacac ttgtaaataa ggtctttttt tctctgcacg ttctttcaat aatacaacaa
    20221 tagcaagaag agaaacaact gagcaaacag ctgaaatagc agatggccag ttaaaatgca
    20281 tattaacctg ttgtatagtt tcactcaact ctctaattgc attttccata aaaactcctt
    20341 tcaaaatcct tccatttgtt ttttaaaata aagctcactt agtttaatct tttcgtacat
    20401 cactcccact cttcttatcc gattgtttga tagtgcaaaa cctttcaaaa taggcattta
    20461 cgagcagttc attttcattc tgtacataat aatccgtatg gtttttctgt tttctcttcc
    20521 attccacttc caattccggt ttattgtgca tttataaaac aagttctatg cctaaaatta
    20581 tcaaactcat aatttaattt tcgccaccat tttagtcaaa actgagtcgg tgttaaaact
    20641 catctttaaa ctaagagaaa ataaaatcag aaatctcctt tgaaatcttt tctgttcttt
    20701 tatttatgtc ttcaacagtc caaacgggtt cagaactatc acgatatgta tcaatcatat
    20761 tcttcgccaa taaaaacatg ctgtctttgt atcctttttt ctctttgact gagctttgaa
    20821 ttttagttct aaaccaagca tttccaattg accggttaat atcttcctca agcagcatct
    20881 tgtttccaag tttatttact acagacaaaa actcttcttc atctgataaa cccgcatcta
    20941 tgcgtatttg aacaatgttt ttaccacttc tcggcataat atgctctatt tcatattttt
    21001 ccggcaattt gaatttttta tcaatttttt tggagtaaat atattcgctt aaatagacaa
    21061 gtgggttctt gttatagtct aaaatttctt tttcaatgtt ttctttgtcc cagtttttat
    21121 taatatgaga ttttatgtct tttttaatct cttcaatttc cgtgttttta tctacaactt
    21181 ttatatttaa cccaaacaaa aaagtcttaa atttagcact tgaataacca atgtcttcca
    21241 attctaaaac cgaaaataat ttcaaaagat aatgcaggaa atcgacgacc aagttttcag
    21301 ttatttcgtc caactcatat ctataaagat aagagatcag atataactta atattctcat
    21361 tgaacttcgc actcatttga acagtagggt aatatttaat tatgtcccaa gtctttgcta
    21421 tttttagcaa ttgagccgtt agtcccaacg aattttttaa taattcctta ttttcttctg
    21481 tataatatct tcttagcccg ggcgttgtta cgcttactgc acctgtttca gatatgtatt
    21541 ccctatcaat agcacgcttg atatacatgt attgcataag gatggaatca atatctacta
    21601 tccttctttg ttcaaggtct gaaaccaatc cctttaattc agtccatttt tcgacaaaat
    21661 cttctctgtt atcccctgaa tttgagtata actttgcaga aatgatgtct gcatcaagaa
    21721 gcggcatacc gtcagaattt aaagaattaa acattgttat cgcttgttct acattccaac
    21781 ttctaatctc gattatttct gacttatcca gtatatactc agaaaaaaca ttgatatttg
    21841 attcagaacg ttcgagtaat tttgtgtaaa aaaacttgaa attcctaaaa ttatttgtat
    21901 atttattatt tctctgttta tatttaattt ttttgacttc attttcggcc tttttaaaat
    21961 catggctgtt taaaattgtt tttaaatccg aagaaaactg ttcatttatt gaaagatttt
    22021 ctaagatatc cgctgtttgt acattttcaa agtttgctaa tatatcataa atgtcttcgt
    22081 ctttagcttt gtacaagatt tttataattc tatctctttt catctcgagg gcacgagtta
    22141 atctttgtga atcttcatct ccttttgtct cttcaatcga ttgatttaag cggattagaa
    22201 gagcctttaa taataaaata aatgttattg tcctttgctg cccatcgatt aaacttaagt
    22261 ttttatcctc ttcttggcaa ctaataataa ttgtcccgaa aaaatatgga tctgtaccac
    22321 cgctatcaat aaaatcttca atatcttgcc ataatttatc acattgtttc tcttcccatg
    22381 aataagccct ttggtattca ggcactaaaa actttgaatc tccttccagc ttcaaatatt
    22441 ctcctatctt ttttagtttt ggttcaatgt tttttgccat ctgtatctcc ttttatttgt
    22501 ccattacttt tcgagcaaac atctatgtct cacgtttacc tcaaaaattt ataaccgcct
    22561 ctactgtcct tgttcaaaat aatattctcc cttattcata atctccgtaa acaccgtatc
    22621 tgaaagccat ctcttaaatt gcggattaga tgtaaactgc aaaaagagct ccatattatc
    22681 cttcatcatc gaacgcatga cgtcttgaag aacaacatcg tattcaattc tgatatttct
    22741 ttcatcagag ttttttattg cgttggaaat acgctcatct ttagcgacca tatttggcag
    22801 ttctctgatt tgtctggcca cattatcctt gtcattccag tcaatatcgc caaaaatatc
    22861 attgaaatta gctataattg tgtcgaggta ttcaaactga gcctcagata tagaaactgt
    22921 tgaaaattgt tttgcttcca actcaccatc cacattggaa agggcaatat ctatttcatt
    22981 tttcttttca agcctataag attcaagatc tattgattct ataagctcat ctatattgtc
    23041 gtcaactcgt ggagtgggca atctatgaac caaaaactca aaaaagatac attttttggt
    23101 ccactcaact tggcctatcg gaaggatagc ggaaagaaaa ttataattcc ttataaacgc
    23161 ttttacgccg gacttaaact caacctgctc ttcttcttta agttctttat atctctcaac
    23221 acaattgtcc ataatcgggt ctatgaactc tctcggttgg tctttgaaaa acatgtcaac
    23281 cactcgctct atctgatcct cttcgtagac gcacaaagca tctaaccttc ccaatatttc
    23341 atatagtttg ttaggatctg ttccctctat cagttttgtt tctttataaa acggctgaaa
    23401 tgcttcctgt atatcttcag gtttattagc aaaatccaga acacaagtgt cgatttttcc
    23461 gggatatgac ctgttaagtc tggaaagagt ttgaaccgcc tttacatcat tcaaaatttt
    23521 atccacatac atagtatgta gaagaggttc gtcatatcct gtctggtact tgtccgctac
    23581 aattaaaaac ctatattcat ttcctttaaa aacagtcgct gtttccttat cggaaaaacc
    23641 gtttaatgaa gcctcagtga cttttttacc catatattct ttctcacctg aaaaagcaac
    23701 aagcgcctta aaaggactgt tatatccctt caaaatgtct tttatagcat ggtaatactc
    23761 aatcgctcgc ataattgagc tcgtaacaac catagccttt gccttgccac ctattttatt
    23821 ggaagaatta tgaataaagt gtttcaccat ctcatcagcc ttttgcctaa tgggaaacgc
    23881 ctgtccttca acataagttt taatctttct cgcagccttt tttgagtcaa agacaggatt
    23941 atcctcaatc gtcttataaa ccttataata attctcgtaa gaggtataat tcttcaggac
    24001 atctaaaata aaaccttctt caatagcctg cttcatagaa tagaggtcaa acaccgagcc
    24061 gaacatctct atagtctttg ctttgggcgt agctgtgaaa gcaaaataag atgcgttctc
    24121 cggcattttt ctcgcttcaa taatggcatt tatgaagtct tccgaatcca tatcatcttc
    24181 tatatgatat tttccggaaa tagcagtaga aagcgaagcc gctgccttac cggattgaga
    24241 ggaatgtgct tcatcaatga taaccgcaaa gtttttgccc ttcatttttg ttccgatagt
    24301 ttcaatgata taaggaaatt tttgtaccgt agtcgtaata atcttctttc cgtctacaag
    24361 caagtcatgt aaatctttag aagattcgga atgagcaaaa ttggaggaaa ctgacataaa
    24421 gtgccttata ttatccgaaa tttgtctgtc taagttcctt ctatccgtta cgacgattat
    24481 tgaatcaaac aaagtcttat cgtctttttc cgccttcacc aatcgatagg cgagccaagt
    24541 tatggagttg gattttcctg aaccggcaga atgttgaatc agatattttt taccgacacc
    24601 gtttttttct atgtcctcta tcagtctctc aactacacgc cactgctgat atctgggaaa
    24661 aatcagagtt tcttttgttt tgcctgtatc tttatccttt ttcttctcca catagataaa
    24721 gtcaacaatc aacttggcaa gcatttttct atccaaaaac accttccata agtaatccgt
    24781 cattgtcccc gtacctatag ggtttcctgc gcctcctttt ccgtcacctt tgttaaaggg
    24841 catgaagaag gtagattttc cggcaagcct cgttgtaaaa gagatattgt tttcatctac
    24901 agcaaaatga gcaatacatc tcttaaatga gaatagtgtc tctttcggat cacggtctgc
    24961 tttatactga tcttctgcat ccctataagt ccagcctgta gaagatgctt ttgattttaa
    25021 ttccatagta aaaataggta ggccgtttat gaatatgcaa atatccagtt ccaatccggg
    25081 atttttctct gaataagtga gctgattggt cacagaaaaa gtattagaat caaattcttt
    25141 gaaagagtcg ggtctctttt tatccaaagc gtgataaaag aagataatgc ctgtaggata
    25201 catttttatg ccgtctttta aaaccttggc cacaccttga gtatgcaagt tggaggaaag
    25261 agtattgaga aatttttgtc ttcctgcatc tgtacccagt ctatgatctg tataagcctt
    25321 gggctgggtt ttttctataa aacgaaagag tctttcttca tctacagcat gtcttttgtt
    25381 gtagacgagt ttcatctttt caggctctac atattcataa gccacagatt ctgaacttaa
    25441 atattcgatt atctgctttt caagcgaact ctcggctttt tgtgttctta tgcccattct
    25501 taatcctcct cttcaaaatc tatttccact tctgtatctt cttcaagtgt atcatctaag
    25561 atttgaactt tttcatattc ggggatatct atattacgaa tatcaacttt gccggtaact
    25621 acatcagata tcaatgtctt cttcaagttt tttaacgctt ggattgtgtt atccaaattt
    25681 tcaatgtact tatttagcga caataactcc gatttaattt tatcaactat ccgcttttgt
    25741 tcatcaaaac tcggtacagg aacatacatt tttaaaaatt tttttgctgg taacctccac
    25801 cttcccatac gtgcagctcc ctgccccaat ttatagaata cccttctttt ataaaatagt
    25861 tgaaacatga gcaaataata ttccggatat atttttttgt cattttttat tctgaaaacc
    25921 ctgtaatccg gactggttac gccaaaatgt tttgaaatgt ctatatatcc tgtcaataag
    25981 tccatatgat tcatcgcaaa gtcaccaact tctacaaatt ggtaattaga ataattctgt
    26041 gccatctgac cttcgttagc acttatatct ttaatcttaa ttcctttttg agtaatagaa
    26101 agaatattat aaccttcttc gcccacaata tttttaacaa tttcaaaatt tcgcttgata
    26161 ctctcgaatt tccaactttc agggatgcta ttagtataat ctgttgtgat attgactttc
    26221 ggaacatttt tgtctaaacc ggaaaacaaa tatttattaa tcaatacttg ctttaaattc
    26281 tcaacactat taatcttagc tctttctaac ccaatcagcc tatcaatttc attgattttc
    26341 caatctaaaa actcggcgat ttgttcttgt tcttcttttg gcgggagtgc aaaattaaat
    26401 gtacctaatt tccctaaatt cacaccattt ctatctcctg tgacagacat tttgtactgg
    26461 tgcttaaatt gattactaag aagtaaaaaa aatacaaatt tgtcatttac ttgtttttca
    26521 gatgaaacac aacataattt atttacaaac gatacttctt tttcaagata tcctattttt
    26581 tcgccaccac ttccaccttc aatgcatact aaagaagctc cttttggcgc aattttaaaa
    26641 cttaaatcat ttttctgaat atacattccg ttattataat caatagtgtt attatctttt
    26701 ctaatatctt ttgttgatat atagggtctt gcgctatcat ttttggtata gtggtctttt
    26761 ttctcatcag gaatactatt tcctgtatat atatccgtaa atctttttaa tgctgcaatt
    26821 tcccagtgtt ccgggatttc ctcaagccaa ggaagatcta cttttttata agctttatat
    26881 ctcataatca agtccctcca aaatagtatc catcaagcct tcggactctt tttccaagct
    26941 ttttatttct tctacaatct ctttcaccga cggcaatttc ttaggttcat agaaatattt
    27001 agtaaaatta atttcataac cgacctgaat agacgcttcg tctacccagg catcttcatg
    27061 gtatgggatg acttcttttt caataaaggc atttatgccg ccatcatgtg tcaaaggaat
    27121 ttgctccgta tcacgcaagt ccgcatcggg aacgagattt ccttttgagt cttttgttat
    27181 cgcagctttt tcatttttct tgacaaaaag gtctaaaagg cttgttttat tcaagttatt
    27241 gagagttaaa ccgcttattc cttttgcttt ttcattaaat ttttcttcaa attccgtata
    27301 cgataaatag tcctccgctg attttaaggt gtccataatt tctaaatagg ctttcatctt
    27361 atcagtatca gcgagctctg tctttgaacc cttagttgat tttacacctt tgtccttata
    27421 ttccttcaaa gctttagcgt caaattcacc gtcaagaaca cccaagcctt taaggatagc
    27481 gttgaattcc gataaggttt cgtcatcaac tctcacagcc tgtctcaaag gtctttctac
    27541 agtcacttga taatagccga attcatcatt atcaaagatt ttggaatact cattttcttc
    27601 aaagtccaag tacaggttta ggattttttt ctgatcgtat tggctgaatt cacaattctt
    27661 cttacccata tttttacgaa gagaagtctt gatagaagat gcgtttatta attgtatttt
    27721 ccccttacgc ctctcttctt ttctgttgga aagaatccaa atatatgttg aaattccggt
    27781 attataaaac atattctccg gcaattcgat aatagcttca ataaggtcat tttctatcat
    27841 atattttctg gcattattag caccgtttcc tactccgccg gtaaatagtg ccgagccatt
    27901 atgaacttct acaatacgtg aacccatttc ggtatctttc atcttagaga tattattaag
    27961 caagaataag agctgtccgt ccgatacatc cggtatcatt cgaaaatctc cgtctccttt
    28021 gtgcgtagtc acaaaacgag agtctatcac attgtccttt agatttttac tttctcctgc
    28081 accgattttt tcaagatcta ttttccagtt cttcccgtaa ggtggattgg atagcataaa
    28141 atcaaaagta tgtcctgcca gaaaatcgct tgaaatagtc gaaccgaaat aaatgttgtc
    28201 agaatcctga ccttgtccct ttacaagcat gtcagctttg gcaatggcat aggtttccgg
    28261 gttaacttcc tgcccataaa tatgtgtact aatggttttg ccttttttct ccgccaaatc
    28321 ctgcattttt tctgctgcaa cagtacacat gccaagtgtt ccggctgcac cgtcataaag
    28381 agtgtaaggg gcatctttta ttttatctgc cacaggaata atggctatat ctgccataag
    28441 ttcaataacg tcacgaggcg taaagtgctc tccagcttcc tcgttatttt cctcgttaaa
    28501 ccttcttatc agttcttcaa acaaaacgcc cattgtgtga ttatcaagac cgggtttaat
    28561 cacttcatct tgactgttaa gaacaggata ggggctgagg ttaatttcag ttgaaacaaa
    28621 tttctcgata actgagccta atatacccgc ttcagtcatg gtgtccaatt gattataaaa
    28681 cttgaattta tcaagaattt cacggacatt cggcgaaaaa ccgtttagat aatcctcaaa
    28741 gtttttcttt aggttttgag cagaggtttc actctttaag tcctttaatg tgaattttga
    28801 aagattataa aagggaagat caatagctaa agccatagca gcttctaaat tttctgcctg
    28861 attttttata aatgtctctt ttaagtccaa aatttctttc tttttaggct ccaatatagc
    28921 atcaaaacgt ctgataactg tcatggggag aataatatct ctatactttc cacgtacata
    28981 cacatctcta aggcagtcat cagctataga ccaaataaaa ttaaccaatt tattatagtc
    29041 tgctgtgctt aatttataat ttttcatcag gtagtctccc tctttctgcg tcgataatac
    29101 aattttcaac aagattcaat tgattttgag tgcttatgaa attgcttaca aaataaaaca
    29161 atctcacagt tattcataaa agtccaagtt tgtgaacttt aatgtgaact ttaacttgtt
    29221 attaattata tcacagatgt gcttttaaat aagattaaag taacttgtaa atagtatgcc
    29281 tattccggtg tggaggagcc aacagtccgg tagaatgaga accaccttcc tttttaatta
    29341 tcttatgttc ttggatccaa tccataacgc ttttgcatta attcattctt gcttttatct
    29401 agactttaaa attaatcttg taaaaatcgt atgtgattct gtccataact acatctgcta
    29461 gaatactatc atcaccaatt ctttcatacc aaccttcttc tctgaactgc gagcagaaga
    29521 ttatggaact tcttttgcag catttgcgga ttaactcaaa atcatgtttt gcttctgaat
    29581 tgttaggttt cacaagcaac cattcgtcaa ttatgagtaa cgtcggttta agatatttgt
    29641 ttaatgcctt agcgtaatag ccttcgcttt tttagtaact tcaatctcca ttggaaaatc
    29701 aggaagtcta acatatcaca cagtgaaaaa ctgtttacaa gcttccatgc aaaagggcag
    29761 accatgtagg tttttccact gcctctagct ctggtgataa aaatgttttg atattgtgca
    29821 atgtttctct cgcaaaaatt tatgcaatat agtcgcagaa gtcttgatat ttggttataa
    29881 ttcttgaccc gcctttatat atatttacgc caatgtgatg catgtggaag gaacaataaa
    29941 gccaacgaaa gaggtgcctc tcaaactctg gacagctggc tcaaaatggt ccggaatatt
    30001 gacacctaaa ttcagttatc tttcttttat aacttagtca ttaatacttt actctgattt
    30061 atcggacagg gtgcactatt taatcggtat atccaatcta ggaaaatatc tttatatagc
    30121 acagactttt agatgaatag ttatgagaaa gaattcaggc tatactccca aatcaagtta
    30181 gacaacaaga ttttatttat cctaaagaaa aagcaaatat caatatgcat atcaacaatg
    30241 tccctgcagt ctcttgctct tgagaaataa agctattaag caagaactgt tttttctact
    30301 gttttttctt tcttggtttt tctctttctt aaaacttatt tacctcaacc caatcttcat
    30361 caacatcaaa ttgctgaaga gcccaagatt tctcaacctt ttttagactt acttttataa
    30421 atttgttttt ttgattgtaa taagtaatat ttatttcatc aaaatcacta ggacactctg
    30481 ttttctttaa aacaaataat aggtcaaatg ctcttatgct ttcagaagga ataatgaagg
    30541 aatatctacc attatctcca taataaacga gaactgaatc tttcttagta ttgtccttct
    30601 gataattaat ttgtttcatt ttcttgattc ttctcccttt tttataaaga acaacaccaa
    30661 aatctctgat gataacggga gtttttttca tattttgtat ttctatccaa attggtagac
    30721 gcataacaat ttcaccattc atattataaa ttccagaagg tcttttatca ctattttttt
    30781 catatacaag ttttctgtaa atagttacat ctcctgtatt atctaaccac ctatcaacta
    30841 atttatgaaa cacatatgca aaactcgaga ttagagctcc aatacaaatt aaaaaaacct
    30901 gttcatgcat attaatacct cactccatca gaaaaaatta ttgaattaat attatgatta
    30961 gttttctcaa ttttactttt atccaattta ctatctgcaa taaataactc atccagtaaa
    31021 ccaaattctt tttctaaata taacaacgac tctaatactt ttgtttttac actagaaagt
    31081 atgctatcag caacattata gccatatttt agtactatac ttatgcacga tattcccatg
    31141 ttttttttaa caacctctgc atactttgtc aaatcatact gcaatgtacc tccttttaaa
    31201 ttctctaatt ctaatatgct attcgttata atgtttagct ttcttacttc atccgcataa
    31261 tcgccaaatg agttaataac aagtggttga ttcttaacca aaaatgaacc attcattcca
    31321 gaatatagaa ttgcaaaagg cagattcttc ctgtaatccg gaatgtcctc atccgctcca
    31381 tatccattta attcattttt agcccattct tttaactctt tgttttctaa ttcatctaca
    31441 ataactaaaa gtttttgcaa tgaaactgtt attgatttgt ctccatctat taaatcctta
    31501 ataatttgac ttctagccat tattatatcc acctcccatt ttataactca gccctacaac
    31561 taagtaatcc cccgcttaac aaacattctt cttggcataa caactaatac acttaataaa
    31621 caagcttaca aaaatttatg catgtgtttc ttataaaatt ttatatgtaa gttcatctat
    31681 aagtttcatt gataattctc gtaatatcat caatgtttct ttattatagc ggaataattc
    31741 acgtgttttt gggtggtcaa ctataaaatt aagaattctc ttatatctat ctataatctt
    31801 aaccttcgat tcatgtggta tatcagactc aattatcctt tctagctcac tagctacatc
    31861 atacaaccac ggaaaatcat cccgatacaa acttaaaata accattaaat cataacacat
    31921 aatattgtca aaatctttct cactacggag catataaaag atttcttccg caattacaga
    31981 tgacatccta cacgaatagt gcattgcatc tacgtcaaga accctataac tctttgacat
    32041 aacttcctca accatcttct gaaattgctc attgttttta taaatcctag catctacatt
    32101 gcctaataag cgctgtgtat tacgcgacat ttcaagaatt tcttcaagaa catccgattc
    32161 taccgtttct aagttatctt cactattttc tgttggttcc aaatgtaaat cattcaaact
    32221 tttatctaaa gttggatacc aaacctcgaa tgtttttgcg agttgctggt cagaaagact
    32281 attttccgca gcacgattta gtgttccaag caatctcttt aaatcttctt tgttaaatga
    32341 agtggcttgg aattgggaaa taggcgaatt ttttaaatcc gagggtttta catcaaatag
    32401 aaatggtgct acataggtat tatccatagt ttttgatagt gcgccggctt caaaatttaa
    32461 ccatggagct aataagtttt cctttgttac acaaatgata ccaaatgtag attcttttaa
    32521 ttctcttgca atatctgtgt tccaccttgc acctttgtct atgtcttcag aggaaacaaa
    32581 tggttcaata gattgaataa ccgatggaag ccaatcatgg aaaattaacg caacttttct
    32641 actccgagta cctgaccaac taataaaaac tttcattgaa taaactcctt tttaaaaact
    32701 ctatcttaat aaaaaaatat tctcttatta tatagcataa tatgcaaaat tttgtcaacc
    32761 acgcctattt tctgtgtaat cccatacaac atactcaata gcaaccttag gcaattgttc
    32821 ctttggcttt atctttgtta tcctatgata atccaatgta attctgttta tactgtctga
    32881 tttgagtttt tcatcaacag caaacaaatc ttctatacta cccctcccga gaagctaatt
    32941 ctttatcaat tcgtcacgca gtgcgtgacg aatttgtctt tattttaaaa tctcccaata
    33001 accataacgc ctaccacctt tacgctctat aattcctttt tcttttaagg tatctaagcc
    33061 tctttgaatc gttcgcctcg ctactgacaa attctcagcc atttccgtcg tagtaatagc
    33121 tttatttttt cttactaagt ctaatatcat ttcctcaatt gtgacatcct ttgtgacatt
    33181 tggctcattg gatgtcacaa tggccctttg gagagcctta aacttaacca ttatatctcc
    33241 gccatgaacg atatattccg ggtcttccgc accatgtgcc acgcatgctt cacggatttt
    33301 ctgaattccg cgaccccagt tttcaatata gccggctctg taaaatacac gagcaatatc
    33361 aggattatat ggctttgata catgtgaacc taaaagagtt tctgccgtcc agccaaaagg
    33421 aagcatactg caattactca catacatcac atcatcttct atcctgattt gcacaggaac
    33481 attctcggcc caattacaat ggattatggc gttgaagacg gcctccctta ctgcctcacg
    33541 tgcaaaggga taggtctcca ccctggtatc tttatagtaa gaaattgcgg cctttaaata
    33601 cttcaaataa atcaagtcaa ttacacggtc tgccataata atgagagagc cgtgtacctc
    33661 atcctgatat aaaatctcac ttccttcaaa tttaccgatc ttaacataac agccgccgct
    33721 gactctctcc ggctgacgat aaaaacataa ggctgcagca cgtttaagtt ttccgtcaac
    33781 aatcaaatcc agcttctcca aaagttcagc gttggaaata tccaaatctt cttttgtcat
    33841 gcggccgctg cgaagcgctt ctcgcctgaa aatatcaaaa ctttccttat ctaaatcttc
    33901 tatgcctaca ttggtaattg ttgcagcatc ccacttacgt cctgtctttt ccatcagaaa
    33961 acttgtaagt gcattaccac gcaataactg ttttgtgcta ccactgcgat aataatataa
    34021 accatcgtaa tttaccggaa acgcccacgg actcacgacg atttcaatat attcatgttc
    34081 tccctctgta agtaaattga catcagccac aatacctaac ttactctgta ttttatttgg
    34141 taaatcgtca agtaagcgtt tagcattttt gacgccggta atgcttccat catcacgtgt
    34201 tccgatatat attttcccgc cctgagcatt agcaaagcca cagatccatt tcaaatattc
    34261 atctctccat gactctttcc actcaatgtt ttggctctct ttcatttctt tgaactccgc
    34321 tttatatatc tcggttttac tttcttatta tatcatataa gacataaaat cttatgctac
    34381 atactacaaa cctatataaa aaatgaataa tgaataatga ataataaata ataaaaggtc
    34441 aaagcaatta taaatctaag tcacctgata ctactcacac ccaattgtat ctggaggttt
    34501 aagaaatcct taatccacta ttcttataat accatacatc caacttattt tatttcattt
    34561 cactttactt taattttgta ctttctctac attatccctc tatataagtc ataaatattc
    34621 gttccaatat ttgtaaatca cctgttatat attctcccta catctgttat catatatatg
    34681 tgtacatttc ctttattgtt atatttacgt gtttcttaga aagcggttag caagcattaa
    34741 aggatgcaca aaaggagatc ttcatgatta cgcaaaaaga atacaataag gcaagaaaag
    34801 caactctgga atatcttgac aaggcgggta ttgttgtgac cgaggcggaa aaagaaagaa
    34861 tagaagttgc ggatttcgga ttaggagact tgaagaacac aggccttcag atactgacct
    34921 atgtaaatac caaaaaggta tgtgccaagg aactggtttt gacaccgttc cagacatgtc
    34981 ccgagcatag gcatcctccg ttaaaaggtt ctgacggtaa agaggaaacc ttccgttgca
    35041 gatacggtga agtttatctt tatgtttccg gtcccgctac gaaaaaccgt aaggtggatc
    35101 ctcccgaagg agtctacacg gtgtttcatg agattatact gaaacccgga gaccagtaca
    35161 ctttatatcc cgacactctt cactggttta aagcaggagc ttcgggagcg gttgtttcgg
    35221 agttttccac ttccagtcat gacgaaagtg atgtttttac cgacccggat ataaacagaa
    35281 tgtcgggtgt tgaagagaaa tagcttgggg gaatgtaacc gtgtctaaac tgtttattca
    35341 atacggtgcg gggaatatag gaagaggatt tataggacaa ctcttttctc aaggaggcta
    35401 tagagtaggt tttatcgatg tcaatactga tatcataaac agattaaatg ctgataagaa
    35461 atataccgta acgattctgg ataatgatat aaaagaggag attgtcttat atgatgttta
    35521 cggtattgac ggaagagata actccgccgt tgccgaagcc atagcctccg ccgattgtat
    35581 ggcaacggca ataggggtta acatactccc gtatatagca cccaacattg caacaggtat
    35641 aaagttaaga taccaaaggg gtattacatc tccgttggat attataatct gtgaaaacct
    35701 tatagatgcg gataaatatc tgtcacagct aatatgtaag caacttgacg aaaaagaaac
    35761 cggatatttt actgaacata taggtttggt aaaggcttcc atcggacgta tggtaccggt
    35821 tttaactgcg gaacagatta aatctaaccc cacgaatgtt tttgcggaaa gatactgtaa
    35881 gcttcccgta gataaagatg cttttaaagg aaacataccg gatattcccg gcatatatcc
    35941 tttctcccct ttcgggtatt tcataaagcg caaactgtat atacataaca tgggccattc
    36001 tttaacagct tatctcggag cgctttcagg ttacaaatat atatgggagg ctattgatga
    36061 cagcagaata aaatatgttg tactcagagc tatgctggag tccgcagggg ctatatcttc
    36121 agaatatgat atgcctttaa gggatttact tctccatatc tatgacctta tgagaaggtt
    36181 ctccaaccat gccctggagg ataccgttga cagagtgggt aaggatataa agaggaagtt
    36241 atcggagaac gacagattca taggagcctt gaagtattgt ctatccatag gtatcaggcc
    36301 cgtttacatc ccggcgggag ttgccgcagg tcttctgttc agagcacaga aggatgataa
    36361 ctccggtgag ttgataaagg cgctggaaga aaaaggtgtt gtttatgtat tggagaattt
    36421 ctgcggttta tccgataatg aaacttattt aaatatgatt acggaatatt ataaacaatt
    36481 gaccgattca tttgatatgg agaagtttat ttataacttg gacgagcgat acgggaaaat
    36541 aaacagggag tcggatatat tgtaggctga tgtggaggat aacatgaatg acatagcttg
    36601 tgtaggaata ttggtagcgg attgtatagc aaagcctgta aaggtaatcc ccgacaaggg
    36661 caaattggag ttggtggaca gtataggtct ttattcggga ggatgtgccg ccagtgcttc
    36721 catcaatatt acgaaaatag gcaaaaaggc tgttattata gggaaaatag gtaatgacgg
    36781 tttcggtatg tttatgaaat cttcactgga atcacacggt gtagatacaa gaggactaaa
    36841 agttgatgcg aatggagaaa cttcttcttc tctggttatg gtaacccctg acggcgagcg
    36901 gtcttttctg cattcggtag gaaccaacgg cacattcaat gaaaaagata tcgattatga
    36961 tattgttaag tcctcaaata ttatattcgt tgcgggcacc atgctcatgc ctgcttttga
    37021 cggaaaagat tgtgcccttt tcctgtcaaa atgtaaagcc ttgggaaaaa ccaccgctct
    37081 ggatacagca tgggattctt tagggcgttg gatgaagctt cttgctccat gtatgccgta
    37141 tatagactat ttcctaccca gttacgaaga agccgtacaa ctatcaggta aaacgaaacc
    37201 ggaagatatt gccgatgtat tcttaggtat gggacctcat accgtagtaa taaagatggg
    37261 tgaagacggc tgctttataa aaagcaaact tacaggtgaa aaacacttta ttgattcttt
    37321 tcgtgtaaat gcggtagata caacaggtgc gggtgacgca ttctgtgcag gctttttaag
    37381 tgccctggtc tcgggaaaat ccctatatga tgcaggtaga tttgccaatg caacgggtgc
    37441 acactgtgtc atggcaaaag gggcttccac aggaatcaaa tcggaaaaag agatggaaaa
    37501 gtttattgcc gattatgatg taggtatact ttaagtcctg ttttacttcc actcttcatt
    37561 tgtaaaagtt ggtaattcag attaaatcac ttaataagtc ttattgttca taaaagtaat
    37621 cctatactat taaattacta aagcattttc tttatccaac aaatcaataa tatcccaaaa
    37681 ttccttgtat ttgtttttaa tcatttcagg accttctcta ttcttttatt ttcatttctt
    37741 gcctcaagta tagctttttc taactctttt cttcttttga ttttcttatc taggttttga
    37801 acgtctttca tttttatata ttcagacttt attttttctc cttctctcca ctgataataa
    37861 aaataagtct tattatttat atttttttta cttaaatatc cgttcggaag ttgagaaatt
    37921 tctttttccg cagaatctat aaaatgattg tttctttcta attcttcaat taacacttgt
    37981 ttcaatatag acatttctct cccctactta acctacatta ttattataat acattttgta
    38041 ggttaaataa cgttttatta atatattaga aacatataaa ccggaaaata aaaataataa
    38101 tatcagggaa cagtaaatta tcttttctta actttccatg ctccctccat agatgaacct
    38161 atgtattcta taaaaccttc ttctgttagt tttgccaaat ctctttctat cgtccttaca
    38221 tttaaggaaa acaccttact taattccggt gctgttgtat ttggattttc aaataataaa
    38281 tctactattt ttcgtcgtct ttcattaggt tctaatttat gaccgacatc gtgaccgaca
    38341 ttgtgaccga catcattcaa atttttcaat actgttgttg cgtccggcac tttggtgtct
    38401 gttacaatag cactttggag agccttaaac ttaaccatta tatctccgcc atgaacgata
    38461 tattccgggt cttccgcacc atgtgccacg catgcttcac ggattttctg aattccgcga
    38521 ccccggtttt caatatagcc ggctctgtaa aatacacgag caatatcagg attataaggc
    38581 tttgatacat gtgaacccaa aagattttct gctgtccagc caaaaggaag catactgcaa
    38641 ttacccacat acatcacatc atcttctatc ttaatttgca caggaacatt ctccgcccaa
    38701 ttacagtgaa ttatggcgtt aaagacagcc tccctgactg cctcacgtgc aaagggatag
    38761 gtctccactc tggtatcttt atagtaagaa attgcggcct tcaagtattt caaataaatc
    38821 aagtcaatta cacggtctgc cataataatg agagagccgt gaagcgatta attgagaaac
    38881 aatggatttc tcctatttga caacaaggtg tttgcgtata ataaatcgac caaaagaaca
    38941 tttaagcctt taggtttttc ccataaagca gcatttaaaa gtttgctgac gcttactctt
    39001 catatccgat tattcgacaa gtgcaatctg cttcggtgat acttgatatt ttcactgata
    39061 tttatttgtt tctacaatac tttccaatag ccgctctttc ttgaaccgac ccgtttaata
    39121 taaccgttgt tacgcaagaa agcgatattg ttttcaacgg ttgtctctgc gcagttaagg
    39181 ataactatta gctgtgccgt agtaatgttg gggtcgtttc gcatttccgc aaggatcctg
    39241 ctgcgacgct cgttcagcgg ttttttgttc cccatgttat tccccagctt cttttccgca
    39301 ttctggggat tatggatccg ctgaaacgga atcgtaacga cgatagaatt ctccctgaat
    39361 gtgaacgcat ccttcccgta gacctctata atctttggaa cacctcttcc ggatttctcg
    39421 ctgatatgaa gctgcaggaa tatctctgat aacttctcat tgaccggaac agattctccc
    39481 aggaagaacc cttccatggt ctgcgcggga ggaattgttc ctctggatag aatctcgatt
    39541 cgattggaaa agaccgagaa cataggctca ttcccgtcga tccaccgatt atgtaggata
    39601 gcattgatga ctgcctcacg aaatgccttg ttgtcgaata gcggaacttc ctttctttca
    39661 acgatccggt ttctttcatc cgtctgaatc agattaagga cgtccccata gcgaagaagc
    39721 tcgtcaaggc tgtatagaag gcaggagttc ccgaattccc gaacagaaaa caacggagag
    39781 cctttcgttt caccgtcaaa aatggaaacg cgcaatggga tgtgggaatt atctgacaaa
    39841 agctgggcca gtatgttgaa ctcaccgctt ttgtttttga gtcccagatt cttgatgaaa
    39901 gtctctttcc tgaggacgat tccccgtgaa ccgtaataac cgaagagttt tgaaaaagtc
    39961 agttcttgat attttgaggg aacggtttct atcgtctcaa ctctgccgtc aaggatcttg
    40021 aaaagctgaa tctctctttt cggataatcc ttcagcgccg ctttggaaga accgatccgg
    40081 atataccgtt tctctttgaa tgcggttggg atctcctcag cagccggaat aacaagtact
    40141 acaacgcggt tgccgtcaat tgtttcttct tcaaatgaga aattaatgct gggggaaagt
    40201 ttccttgcga ggtaattctg aaacggctcc ttattatgct cgcaatactg attgaaagtc
    40261 gttccaacga tttcatgagt atcattgttt acgccccaaa cgaagtatgc atacttctta
    40321 tagtgaaaag cagccgcatt cgacatggcg gaaacatatt cacccagagt ttccggttga
    40381 aaccagtttt ccttgaattc aaaccattcc tgctcatcgt cgtaagcaca gagatctttt
    40441 acgattgact taatatccat atcagtgtct gctcctttcc ccaagtttat tccccacatt
    40501 cgcattgcaa catgtgggga ataaaaattg aactgatttc agaaagtttt ttatcgctcc
    40561 catccgcgac ttctctgacg ctgtcgtttc tgaacctgct gagctgcttt tgcacgttcc
    40621 tgtttcttaa actcgattat gctcaagtgc tcccactcaa aaccgtaaca ctccatgccc
    40681 tgggcgagct gttccttttc ggactgaacc cactggttac gctcatgaaa gctttggaaa
    40741 tattcatcca gggcaagctt ggcctgctct ccgatctccg tagcagagcc tgtgtcatct
    40801 ttttttccaa tctgaatcac cagttcatga tagacctgct tgattggagc attacaatta
    40861 ttgatattga gatgagtacg agtagaatcc acattggccg cattgaactt catggacttc
    40921 ctatgacata cacagattac tgcgaaaata ccctccgaga aactgagcga cgccctggtc
    40981 gaagctttca agaaaaaact gaaagactga catattccac cgtcctttca agcaaaatga
    41041 aatgagctac ttgactttca aatcagatag ctcttatttt tcgttattcg atggagtatt
    41101 tcagatgttg ccaaacgcct ttttagaagt tcagaaatcc ggtatttata cgggtcccgg
    41161 cgttttcgcg gttactccca ctcaatagta gcaggcggct tccccgttat gtcaaacaca
    41221 actcttgtta tgcctttgac ttcttctgtt attctttcgg atatctttgc taatgtactg
    41281 tgcggtatct gtgcatagtt tgctttcatg aaatccacag tttccaccgc ccttaaagcg
    41341 agtacatagc cgtatgttct ttcgcctttc tgtaccccta ccgttctggt gtttgtcaga
    41401 actgcaaagt attggtcggg cttctttttc agtttgtcta tttcttctct gtatatgtaa
    41461 tccgcttctc ttaacatatc cagtttttct ttggttatat cccctattat ccttattgcc
    41521 aatcccggcc ccgggaacgg ttggcgttga gttatggaat cgggtaaacc taagagtttt
    41581 cctacttctc ttacctggtc tttatacagt gctctcaaag gctccacaag gccggtaaag
    41641 cccattttct ccggtaaacc ccctacattg tggtggctct taatagttgc agatttaccg
    41701 tctcccgact ccacaacatc ggaacgaatg gtaccctgag caaggaattt aatgcgtcct
    41761 aattttaaag cttcatcgtt gaatacttcc acaaattccg cccctattat ctttctcttc
    41821 ttttccggat cggttactcc ttcaagtttc ttcaaaaacc gctccgatgc atccactgct
    41881 ataagtttca ggtccttgtt gccgaaaaca cttattacat cttttgcttc atttttacgc
    41941 ataaagccgt ggtctacaaa tatacataca agctgattgg gtatcgcttt ggatatcaag
    42001 gctgcacata ctgcggaatc cacaccgccc gataagccta ataatacctg ctcattccct
    42061 acctgcctct ttatatcttc aattgtttta ttgatatagt ccttcatggg tcctcctttt
    42121 gtttcatgta tattatatag aatttatgcg gtgattatat agcaaattgt gcatatttat
    42181 attttcctta aattcaaaaa accggctttt aaagccggtt tgtttgcatc ggatatatta
    42241 ttcttcaata gtaatctttt tctcttcttc cttgaagtaa tcttcgtagt attcatgcct
    42301 ctcctgctct ttctgtttat tggctctata gaacaagtag aaggaaggaa tcaggaatgc
    42361 tacacctaca acataccata cccacatatg ccatcttgcc atatactgct cattcatata
    42421 tacaagtctc atgaacatac caaaagtaat accttcaaaa cctattgcaa aaataactat
    42481 tgcccatttc attatctttg aacttttgct attctcatcc attatatcta cctccccgag
    42541 tgtggcataa atattaccta ttactcaaca aattatatca ttcgcgtctg ttactgtcaa
    42601 atgatttacg gtgtttttta aactttgttt aagttgcata attccatgtt ttaaatctat
    42661 gcgatttact aaatattaac ggcaatacct ttgtcccgaa gagccttttt aagttccgtt
    42721 atgtccaatt cgtgaaaatg gaacagcgat gcggctaacg ctgcatcggc ataccctttt
    42781 gttaagacat cgtagaaatc ttttatgctc cccgctcctc ccgaagctgt tacgggaata
    42841 cttaccgctt gagaaaccga ttttgtaagc tccagatcaa aaccgttctt tttaccgtca
    42901 cggtccattg atgtaagcag tatctccccc gctcccagct cctcacactt ttttgcccaa
    42961 tcaacagcat taaggttggt ctttttcctt cccccgttta tatataccgc ataccttccg
    43021 tcctcttgct tcttggcatc tatggcggct acaatatgtt ctctgccgaa cattgtgctt
    43081 gcttttgaaa gcagatcggg atttaatacc gctgcggtat tgaccgatac cttggttgca
    43141 ccggctttca gtacggattc aaaatccgca atactcctta tgcccccgcc taccgtaaaa
    43201 ggtatatctg tttcttctgc aacctttttc agcattgtaa gcgtattacc ccgcttttca
    43261 cttgaagccg ttatatctaa gaaaaccagt tcgtctgcac ctttttcaca gtatgccttt
    43321 gcggtttcca caggatctcc ggcatcggta aggttgataa agtttatgcc ttttaccacc
    43381 cttcctttat ctatatccaa acagggtatt attctcttta ttcttccctt cttgtttaca
    43441 ctcatccgct ttactctttt ctttacttaa gaatatgtaa ataattcttt caaatgtatc
    43501 tttccttcat atatcgcttt tccggttata actccgtaag ctcctgtttc tttaacttct
    43561 cgtatatctt ccatatcctt tatcccgccc gatgctacaa catacatatc tgtcttttca
    43621 atcatttctt taagtgcatc aagattaggt cctttcaatg ttccgtcctt ggatatatcg
    43681 gtatatacaa ccgattgtac tcctaaagac tgcataaaca gtgcggtttc tatggcatct
    43741 ttcccgctgt catcggtcca gccgtcggta gagacaaatc cgttacgggc atcgattcct
    43801 accactatct tttttccgaa ctcttttacc gcttcttcca ccagggaggg attcttaatt
    43861 gccgccgttc ccagtattat cctgtcggca tttgccgctg ccagttccct tatccttttt
    43921 attgtgcgca caccgcctcc ggtctgtact gaaagacctg tttctttaca tattctttta
    43981 ataacttcaa tgttctccga cttgccggtt tttgctccgt ccaagtccac aatatgtatg
    44041 tgggaggcac cggcttcttt aaaagttaaa gcggtcttta cggggtcatc ggaatatttc
    44101 gtaacctgac cgtaatctcc tttatacagg cgcacgcagt ggccgttaag tatgtctatt
    44161 gcgggatata taatcaaggc tgttaaatcg ctccttttgt cgacggtatg ccggtttctc
    44221 tgttgtcaat gcttactgcc tgtcttaagg ctctggcaag tgctttgaat atgctttccg
    44281 caatgtgatg tctgttgtga ccgtactgta catttacatg gatattggca cctaagttac
    44341 ttgcaaatgc ggttacgaat tcctccaccg tctcaaaatc catctccccc gttctgccgc
    44401 cgcttacggg gcagataaat accgtatacg gtcttccgcc caaatccacc gttacatttg
    44461 taagcgcttc gtccatggga acggatgcaa atccgtatct gctaatcttc tccttattac
    44521 ccaacgcctc tttcatagcc gtagccaatg ctatggcggt atcttccacg gtgtggtgct
    44581 cgtccacctc tatatcgcct gttgtcttta ctgttaaatc catcaaagaa tgcttggaaa
    44641 acaactccag catatgatta aaaaaaccta tcccggtctc gacggtatat ttgcccgttc
    44701 cgtcgatatt cagttttacc gacacatcgg tctctttggt ctttcttttg aatgtcgctt
    44761 ttctcattat ttcataatct cctttgcggt atttatgagg gaatccatat cggaatcggt
    44821 tcctatagat attctgacat agtcgggtat atagttccac tgtctgataa gtataccctc
    44881 atctttgaac tttgtatata tcgtgctcga atcataacct ccgaatttaa caaacagaaa
    44941 gttgctctgt gaaggtaaca cggtacaccc cagtttatcc agttgctttg caactcggtc
    45001 ccttgtcgcc ttaactttgt tgtttatcat gtcataatac tcctgattat ttagagcggt
    45061 taccgcgctt atctgagcca gtctgtctaa aggataggaa ttgaaacagt tctttacggt
    45121 attgagtgca gcaataagtg ctttatctcc tatcgcataa cctattctca tgcctgctaa
    45181 agagtaggat ttggaaaagg tcctgaccac taacagattc ttgtatttct tcacatattc
    45241 cgccatggac ggctgatttg caaaatcgat atatgcttcg tctaccagta tcagtctttc
    45301 tttgtctttc tccaataaag aacatatata ttcattgcta agcagctcac ctgtcggagc
    45361 attgggattg gctattatca acggatattt acacttactc acggcttctt tatttatagt
    45421 atagtcatag ttcatttccg gtgtttcttc ttttgcatca tacaatttgc aatataccgg
    45481 atagaaactg tatgtaatcc tggggaatgc cactttcttt ccggaaaaga aagcattgaa
    45541 agcaaatgca agagtctcat cggaaccgtt ggatacaaag accatatcgt tgtctattga
    45601 atatttcttt gctataaccg acagaagctc tttactttcc gggtcgggat aaagtcttaa
    45661 tgtttcgccc gctttatctt ttatggcttc cagtacttta tccgagggcg gatagggatt
    45721 ctcattggta ttgagtttga tatatctttt atcgttaagc tgctctccgg gggtataagg
    45781 agtcagtttt tttaatctat cggaaaacag gttattcatc ttttttctcc ttaaaacgta
    45841 tattcaccgc attggcatga gcatccaacc cttcacattg tgcaaacagc gatatccctt
    45901 tatatgctct ctccaacgct tctttatcat actgtatgac actgcttttt ttcataaagt
    45961 cgtaaactcc caacggcgaa aagaatcggg cggttcccgc tgtgggcagt gtgtgattgg
    46021 gacccgcaag gtaatcacct aacggttcgg gtgaataagg tcccacaaat accgcaccgg
    46081 cttgggtaat atagtcggaa tagacctcgg cattttcggt aataatttcc gcatgctcgg
    46141 gcgcacattc gttaagcaca tcaatacatt ccgtaatatc ctgtccgata tatatggcag
    46201 cgttattctt aatactttca ttaatgatat cgcttctttc caactctttt gcctgtacgg
    46261 ttatctcttt tattacttcc ttcgccagtt tcttggatgt ggtaagtaat gaagatattg
    46321 agagtttgtc atgctcggcc tggctcagca tatctgcggc aacaaatctc ggatttgccg
    46381 tttcatcggc aattatcatt atttccgaag gcccggccac catatctata ccgcagtatc
    46441 cgtatgccat cttcttggca taggcaacat atatgttacc cggcccggtt atcttatcca
    46501 ccttcggaat tgtttcagtg ccgaatgcca ttgcaaatat tgcctgagct cctcccgctc
    46561 tgtatatctt tgttacatgc gattcattag cggcaacaat gcgtgctgca tccaacggag
    46621 gagggctaca catgactatt tcttttacgc ctgcaacata tgcggggact gcattcataa
    46681 tgactgaaga tatgagaggt gcggtgcctc ccggtatgta tatgcccaca cttcttaacg
    46741 gtgtgacttt ctgccctatt tttacgccct cttctttatt ccataaaaaa cttttctctt
    46801 tctgttttat ggaatatgtc gccacattca gtaccgcctg ctgtattacc tttaaaagat
    46861 ccttgtctat ctctttgtag gcttttttta tctcttcttt tgtaacaagc atatcctcag
    46921 gcgcaatatc ggttttgtcg aacttctttg tatattgcat caatgcttta tctttatctt
    46981 ttcttatatc ctgcataatt ttgtatacta tatcgtaaac ttctttatta tccagaaatc
    47041 cgtcttttcg cacctttgct ataaaacccg ctttatcctt atcaaatatc ggaatcattg
    47101 cttaactcct ttttcaatgc tgagattata taggatatct tatcggtttt catgttcata
    47161 cttactttat ttactatgag gtgagcggaa atgtcgcaaa tcttctctaa caccacaaga
    47221 ccgttggcct gcagtgtctt tccgctttcc actatatcca caataagttc cgaaagatta
    47281 cataacggag ccagttccac cgatccgttc aacttgatta tctccacatt ctcatttttg
    47341 acatttctgt aatattcgct tgcaatcttg ggatatttgg tggcaaccct cttgttcatt
    47401 atcttatcgg ttctgttaag atacttaccc tctcccgcca cacacatcct gcaggcaccg
    47461 aaacctaagt taagaacatc ataaacattt tttgattctt ccatcagagt gtcttttccc
    47521 gcaactccca tatcggcggc accgtattcc acataaaccg gcacatcggc cgcctttacc
    47581 agaaatatct taatcttgtt tgattgatcg gtatgtatga gctttctgga atcgtcatct
    47641 atatccgtac agggtatccc tgcttttttg aaaagttcca cggtctgtac cgccagtcgc
    47701 cctttgggca gcgcaatttt taaatattcc ataatgccct ccactttgca aaatcggtta
    47761 tctccgtttt gccgttactg cggattataa catggttggg cgctatacag cgtgccatcc
    47821 ttgaaatatt ccgctccttc atatatcttt caaggtcgtc actgtccggc aacacctgta
    47881 tgtttttatc ttcctcaatg tcttttgtta cggataaaac ataatctctc atactttccg
    47941 aaaaagacac tattgcaggc ataaccggca gctcttcaac ctgtccctgt ctttccaaag
    48001 ccttgatggc catttccaga ctcaaggaaa agccggtagc ttccagcttc tttcccattt
    48061 tttccgccag attgtcatat cttccgcccg caagtatggg aaagcccatc tgatatgtgt
    48121 taccgttgaa aatcattccg gtgtaataac ccagcttttt aaccatggat aaatccacgg
    48181 taacatatcc gcttaaaccc atcttatcca gtatgtccag aatctgctta agattgttca
    48241 aagcctgttt tgatcttaag ttaagttttg tttcaagtaa tctgtcaatc agtgatttat
    48301 ccccgaagta tcccgttata gcggttaagt ggttgtatat atccttatct atttttcttc
    48361 cttccaagaa ttcagagata ccgaaactgt ccttgctgtc tatcattttg gatattattt
    48421 cactttcttt ctgtgtaaga gaagcttctt tggccagacc gttaaaaaac tccacctgcc
    48481 ctatctgaat agatatgtca ttaattccta ctctctttgc cgtactaaca gccagcttta
    48541 taaccaacgc atcgtaatag ggattatcgc tgcctattat ctctgcgccg cactgggtaa
    48601 actctctctg ctttccgcct ccgaattcat cggcccggaa gcatttggct atgtacgaga
    48661 gcttatacgg taaagcttca tagcgaatct ttgttgccgc taccctgcat gccggtaccg
    48721 tcatatccgg ccgtaagacc agaaggtttc ccgttttgtc tataagcttg aatatgtctt
    48781 tttgagacac atattcgttc tctccgtaat acatgtcaaa atattctaag caaggtgtct
    48841 ctatttccgt aaagccgtag ttacggaaaa gttgtctcac cgcctgctct ttattcttct
    48901 tggtaaagca tttgctgtct gttaagtcgc tcatgccttc gggattgtga taagccattt
    48961 gcttttctcc ttacttcact acgctaaagt aatgaattga taaagtgtct acatattacc
    49021 accggtattg tacactgtca actgttaaat taaaatatcc ggggaaaaaa gcagtctgaa
    49081 aattatagca gaagactatc accttaaaat atagtattta aaaatgctga atgttattat
    49141 atataacaaa atatgcttta ttttgtattt acagattata attgacacgt atatgatatt
    49201 tatattatca tattaatata atctgatagt tttttgtggc atgaatagga ggataagtgt
    49261 tatgaaaaaa ctttctaccg ccaaattagc tgaaataatg aagacaagaa gaaaaaagtt
    49321 gaatttgaca caggaagatt tgagtaaaaa gacaaaaata aacaggtcaa ttataagcag
    49381 gatggaaaag aataatttca taccttctat aatacagctt gaaaaccttg caaaggctct
    49441 tcagttcgac attgaaaccc tgttttatga tgatactgaa aaccacacat ttacggcttt
    49501 aagaagcaag gagttatcag cagaggaaga taatgaactt aagaagcttt atgccttaat
    49561 ttttatactt caaaaacaga tagtcttatc ggaaggtatt gagaaatgaa cgatatcaat
    49621 accatcttaa ccgaagatga aattgcagaa ttacagatgc ttgcttctga aaaaaggaca
    49681 tctttatttt attcaaataa tgtacctgtt gcaaacggtt taccgatgat tcttgacaaa
    49741 ttaaacattc acttacttga atatccgatc aaatctttaa acggatgtaa tgcattctcg
    49801 gctgtaattc tttgcaaccg tattaatggc aggaaatata ccttcatggg tatcaactct
    49861 gcaaattcat taacggagca gtgttttgat attgctcatg agttatatca tttctttact
    49921 gacaatggca taaaatcctg ttcagaacaa gaaaacaaga gggatgaagc aaaggccaac
    49981 agatttgcgg ctgagttcct tctacctgaa accgctttaa agagaatctt gcttgatgag
    50041 tttaacaaaa cttctttaca tgatattgat atctttgttt taataagact aattgcaaga
    50101 atacactgta actggtggct tccgtatgta gctatagtca gacgactatt tgagacacag
    50161 gcaatatcac aaaaacaatc tgatgctctt ttatttccgg acaataatga ttattacaag
    50221 tattttgaaa gaatttgcca tgctttagac tcagatacat ttctgaaact taatactccg
    50281 acaaacaaga taggaatttc cccgaaaaat attgagatta ttataaaaaa ctatgagaat
    50341 gatttaattg atgataacac attcataaaa gtattaaaca tgttcaatat agaccctgcc
    50401 tctttcggtt acgacttcga aatatgcgac gatgactaca atgaattaga ggaagtactg
    50461 gaagaggtag attcatgaga gtagatgcta cacgacacaa ttccgcaatt aacagcataa
    50521 taagaaatcc aaatcagatt atttttctgg atgcaaacat cttaatcccg cccggcagat
    50581 tcaataatat ctttaataag gacaaatata tatcgttttt ccttgaaccg ttattcacta
    50641 cttttcaggt ttagcaattc atgaagctgt acagcttgaa gttttaaata gtggaattat
    50701 tgattttctt aatacacaaa tatcgagtaa tccttgcaag ttgaaagtct tcaatgatga
    50761 gagtttgtcg tataacgagc agatattaag aaatacaatt gagagtgaaa taggaaactg
    50821 tatttcttat tcagctttgt acaatgaaaa gcaagaccgg ggagaaataa aaaccttatc
    50881 ttttatggcg gtaaaggagt ataagtattt agtgtcaaac gataatgact gtgtaataat
    50941 agtgaggaat aagcttccaa actgctcaat tatcttcaca tatgaactta tttatcttct
    51001 ttcagagata tatccgaaaa aagcggagga cttgcgtaaa ttatacaggt tcttatatta
    51061 ttccatcagc aaggataagc aacacaatcc gagccggagt gattttaaga caaaaatgag
    51121 catactttat aaaagcagcc tgccaatctt aaaggaaata tcaaaacaaa gacagattta
    51181 attaacagaa aagcaagttg cttttccctt cttatgctta tgtattactg tcaacggtta
    51241 aatttaaaat atccgggaaa aacccggaat attcttaaat catatattta caatcctttg
    51301 ctatttaacc gccatagcct ttactctttc caatgccttc ttaaccggaa gtaccgatat
    51361 gagggcaacg gttatggctc cttcggcaag tatatatgaa tagttgtata tgacgggata
    51421 aagcggaatg agtttttcgg ggaatttttc cggtatccag tccataaaga acaggtatcc
    51481 tcccaccgtg tgaaaaaatc cccgtgcaag tacggctacg atataacctg ttaaaagacc
    51541 gttcttttta tctttgaaaa gtcccgacag tcccagtgcg gtaaaagcaa acaggtaatc
    51601 aaaacacacc tgccagaagt ttatgatata cggttcctgc actaactgca gaagaccgta
    51661 tgtgaaagcg gcaataagac cggtaccggt tccgtaccag taacctgcaa ggcatatgaa
    51721 aaacatggat aacagcgtta ccgaaccgcc cagaggcata ctgaacaacc ttatgtacga
    51781 tgccacaaag cctgcggcaa ccgccatgga acaaaaggcc agtttctttg taggatttac
    51841 cgatagttcg gatttttcct tcttgggtcg gaaaaagtaa attgcacaaa ataccaagac
    51901 agccaacaac gctattgcga tatatccgta tgttgtcagc agataattgc cgtccgattg
    51961 agtcaaaaac aaatccataa aaatacctcc cttgtttata catgcaggag gtgaaacagt
    52021 actgttgatt gcttccttac gccggtatta cccggatcaa gtaatgaggg tcaataatat
    52081 ctctcagcca catggctccc ctagcatata tgccgatttc tcggtgaatt tgattctatt
    52141 acaaggaaat attaatgtca agcatattat gaaaaccgaa accttcatta agcattgggt
    52201 aatcatcttt tttgatatat aatttagaag aggcagcaat gatttcaaca aggatgtgca
    52261 catataaaga taaagcggcc atgaagaagc tctggacgga gcttttcggt gacagcaaaa
    52321 gcttcaccga ctggttcttc gataacagat tttctccttc ggacagtgta tgtacttatg
    52381 atgatgagtt attggtatcg tgcgctcatt cgcttcccat gcctgtttta atcagaggta
    52441 aagaaatacc ctgcgccatg atatccggaa tagccaccgt tgaagcatac agaaaacaag
    52501 gcctcatgca taagactttg ctatatttaa taaacgagtt aaatgcaaaa ggcatttatg
    52561 cagccacatt gaaagcggta aatctttcaa catattattc gatgcactac tacggttgta
    52621 ctttaagtgc atatatccgt tcgggtaaga aagataaata tgcctgttta atgccctata
    52681 ttgaaagaaa agcaaacagc gaggtttcat gcaggcaaat agatatatac aatgagtttg
    52741 acggtttaca tgcctgttac acgggattta caaaggggta cagcggtatt gcaataagag
    52801 ataaggcagc gtttaagtta aaaatgaatg attacttaag cgataatgcc aaagcaattg
    52861 cagtgtttaa agacgggaaa ataacgggat actgcattta ctttgatgaa aaagatttat
    52921 atgcggaaga atatataacc gacgatttat acagcgcatg cgccatgttt aaggcactta
    52981 aagaacaggg gaagagtata acggtaaaga cttcaaagaa agttgcggat cgggtggggg
    53041 ttaagaatat gaaagttacc gaacaaaact ccatgggcat aataaatatc ggtaaactgc
    53101 tggaaagcat ctgcggtaac ggcgattacg gtgtaaaggt ttttgacagt ttctataacc
    53161 ccaacaacgg tatttatctt ttaaacggta agaaagttaa tgataaatac catctggagt
    53221 tgtcttcggg acatcttatg cagttactta cggggcataa gtcattggaa gagctggtgt
    53281 cggagggtgt cgccgttata tatgataaag ttgcatgtga tgaaatatcc gacatgcttc
    53341 ccgaacaaaa ctgttttatt gcagatgagt attaaaccgt ataccgttta tttaaagact
    53401 acgctttgaa gataaagtcg cattgatctt taatcttaaa gctttcaata tattcattct
    53461 ccgaaggaat ccacctgttg gtaaaacttt tgtacagctc cccgcttctt tttaatattc
    53521 ttttaaactg cgtctccttg tctatatcca tgaatatacg taaatcatat atatcgccga
    53581 aatacgggtg catactgtaa gcgccctcaa ttatattcag tgtctttgca catatcttta
    53641 tctcactcat ttcgtttgta tggcagttgt aagcatgatg ggtaaagtct gtaggtgatt
    53701 tcagattggg aatcacttct ttttgaaatc gctcataatc tatattgccg cccggctgca
    53761 gcagtctttc catcgttctt tggtgacttt gcaaaaagaa atggtccatg tgaataacgt
    53821 tacagtcaaa tattttggaa aacatatcac caagataact cttgcctgcg gtactcttac
    53881 cgtcaatggc gatattcacc gttgttccgc ttattcttgc tttgttttgc agtttctcga
    53941 cttttgccca taatttcatc ttatttgcat attccgcacg tattaccctg taagaaggat
    54001 tgtataacgc tttatacctc tcactgtgac ttattaaacg gcatcccgcc gcataataac
    54061 tttccttaaa tgtataaaac tcttcttcag ttatgggaag ggatgactcc tttaccgctt
    54121 tctccaaagc tttgacttta tcggaaaacc cctcccgagt accctttgat aaaagcctgc
    54181 tctgcatcat tatttccgta aataattcct gtgaaagacc gatcttttta aggtagccca
    54241 agtgtaccct cacattgccg tttccgatat cttcaatatc ggttttattg ctttttttgt
    54301 cctctatttc gtctttaaga gcaataaaat tgctattttt gtcttcgggt atatgagcat
    54361 taccgaactc gctctgacag aataatttga accagtcggc catttccgac tcgggataaa
    54421 gtttgtactg ttttttaata attctttcca ttatatcttt acggttttaa tcttctccgc
    54481 gtaatactgc accagctcat atttggtgtg cggcggcaac ctgtgatcgg gacaggggat
    54541 gtacccgccc agggctatca ggggcttcaa cctctcaatc tcgtcatcca cagccttttt
    54601 atcatatgac aggacgttct tgttcattcc gcccactcct cttaattctt taccgtattg
    54661 ctctctccag ggagcaatgc tggcattcca gacacccacc tctatgggaa acatggtatt
    54721 aactccgttt tccagccaca taggtatcag ctgatcgata cagccgtcac agtccaaaga
    54781 tattattttc attccgtatt tattggcaag gtcatttcgc tttttgtaat gcttggcaca
    54841 cagattgtcc agaatgtcgg gggcgaaaag aggaccgttt ctgtaacata tatcttccca
    54901 gtagtgagcg aaatcaaatt tgaaatcggt tatctgcagt gttctttcag ccaccttgta
    54961 ttgcagctcc gcataatcgg acactatttc atccagcagc tcatagtcat catatatcat
    55021 gtaactcatg cccattaatg tgagcatgtt acgtatatct cccgctacac tgcccaattc
    55081 cagccctatg ggagcatcta tctcgtccca gttattgttg ttgaaataat cggggtcaac
    55141 acggtcttcc gtatactgta atttaggctt gaacagttct tcataagcct tcctgtcttt
    55201 taataaataa tcgcattctc cggatattga cacaataccg ggtttcacta tctctataac
    55261 accccaactt gtaagcactt tcctggaacc gtcgggaagt tcttcaagaa cttcactttt
    55321 aaaagaaggt cgtaaacttg ccgatcctct tcttgtggta cgccagttaa aatcccaacc
    55381 gagcttggca ttgagccggt aatcggcatc gttgccgtca cctacaccta caatttcctc
    55441 gggtttcaga tgtcccagtt ccacccattc ctgcagcagt tccttccagt aaccgaagtg
    55501 aactgcgggc attctgtccg catccctgta attgagaata ttcatagctc ttgttgtgtt
    55561 atccatacgc caccgcctat agagagattt tacaattatc gcaaaggcaa gtcaacctaa
    55621 actcaccttg aaagcataag cagccggaca ggtcaggtcg ttattgatat aaataaggtt
    55681 tattacagta ccttggacag aaagtctttg gttctttgat tttgaggatg ggtaaacagt
    55741 ttttcaggcg tattttcttc cacaatcaat ccgtcatcca taaacagaat tctggtgccc
    55801 acctcttttg caaaacccat ctcatgggta accacaatca tggtcatacc tgcatcggcc
    55861 aacttcttca tcaaatccaa cacttcccct accatctccg ggtccaacga tgaagtgggc
    55921 tcgtcaaaaa gcatgacctg cggttccatg gctaacgcac gtatgatagc cacacgctgt
    55981 ttctgtcctc cggaaagctg tgaaggatat gcgtcggctt tatcgcaaag acctattctc
    56041 tgcaacagct ccattgcctg ttcttttgcc tgattttcgg ttttaccttt aagctttaca
    56101 ggtcccaaag taatattttt caatatgcta aggtgcggga aaagattgaa gtgctgaaat
    56161 accataccta tattcatacg caacttgttt atatcataca atctgtcggt tataagttgt
    56221 ttattgtagt atatttcccc cgaagtggga gtctccaaca tattaagaca gcgtaagaat
    56281 gtgcttttac cgctgcccga aggcccgata atgactactt tttcaccttt ttgtatagtc
    56341 tgatctattc cttttaacac ttccaacgac cctttaggtc cgaagctttt atgtaaccgt
    56401 ttaacctcta tcacttttag cgagcctcct ttccagctta tttaccagat aagtgagcag
    56461 catgactata cccagatata tcaatgccac tgctataagg ggcataaatg cgtcatatgt
    56521 cctgctgcgg ataatctctc cgccttttgt cagatcggcc aatgcgatat agccggaaac
    56581 cgaagtctcc ttgagtaaca ctataaactc gttacccaat gcgggaagaa tgttcttaaa
    56641 tgcctgcggc aatactatgt accacatggt ctgaccgtgc gtaaagccca aggatcttcc
    56701 ggcttccatc tgtccgggat ctacactcat tattcccgaa cgcactattt ccgcaacata
    56761 tgcggcggaa ttcagtccga aagccacaat cgcagccaat accttatcca ctgcaacggt
    56821 agcaaaaaca acataataga ttatcagcag ttgcaatacc accggtgttc cccttattac
    56881 cgttatatat cctttacaga ttgcattgag aactttaaac ttttttgtct tgtcatgagt
    56941 tgtgcgtacg accgccatta aaaatcccag tataatacct atcataacgg cgaagaaagt
    57001 tatctccaaa gttaccagta atcctttggt aaggtactgc catctgttgt cggttatgaa
    57061 gttatttaca aagcttattt taaactgctc tataaactcg ctgaaattct ctttcatcat
    57121 tacctttccg acaaacatcg gagtcggaag atatccgact ctgacgcctg tatttaaaac
    57181 tacttcttcc ttactattat tacctgtgta gccgtagtgt atgaatcggt gaaatccaca
    57241 cttataaggc gatcttcggt aacggtcatt ccggccactc ctatgtcggc ttttcccgac
    57301 tgaacggcgg caataattgc gtcaaaatcc atattctcaa taaccagctc cattttgagt
    57361 atatcggcta ccgcttgcgt aagatccaca tcaattccca ctatggtatt gccttccata
    57421 aattcatacg gttcaaactc cgcatttgta gccattgtaa gcttaccgtt actgtaatcg
    57481 gtgttatagg aaacataggg actcttgcct tttgtatcat ctcctatata gttggagata
    57541 atggcagcca gtgttccgtc cgacttcagt tgagctattg ccgcatttat cttatcctta
    57601 agttccgaat tggattttgc tatacacata gcataatttt ccagctcaaa cggatcgggc
    57661 aatatcataa ggtctttatt ctttgccgta aatgcttttg ccggttcgtt gtcgataatg
    57721 acacagtcca ctttaccttg tttcaaagcc tgaacggcat cggcaccttt attgtatctc
    57781 tcaataacgg aaccttccgc ttcatagtcg gaagcatata tgtctcctgt ggttcccaac
    57841 tgaaccccta taactttgcc gggcagatcg tctgcggaaa acaccgtatt gggtgctacc
    57901 tgtgtgcagc ccgtcaggac agacagtgcc agtattgttg ttaaaacagt tgctataatc
    57961 tttttcataa tatatattac ctccgtaagt aatttcatgt tttcataaat atacacttat
    58021 atgcataatt agtcaatagt aaagttgaaa tttcactata tttttccgtt tttaataaaa
    58081 aatctaaaaa accaatgaat attattcatt ttcattgcat aaattgatgt ataaatcatt
    58141 aaaaggcaag gaatatataa ggaaatataa aaaaccgacc ggagtcggta tgtgaaatat
    58201 gttgtatact gctctaatta acgggcggcg tcggagattc ggtaccttcc ggttgctcac
    58261 ctacaggctc gacgacctcg ggttcttcgg tttcggtatt ctcttcacct tcttcaggat
    58321 gtttatgatg caaaggacat acggccgtca tgtccacttc atataccaca tcatacggta
    58381 ccctttctat ctgccatatt accttaccgt caacggtaac ggagaatgtt tcctcatgaa
    58441 cctttttgaa tgctccgccc ggtcgcacca gacgcgtcat tgcccataca aagctggcgg
    58501 gacagtcatc gctggcaaca taaaaccgcc cgtattcgtc tttgttgtcg ggatatgcac
    58561 atacaacaac ctcctgatgc acatcgcatg tctctttggg ttcggtacct tcaatgaaat
    58621 attcggtaat gacggttttt ctgccgcgcg gatcggatgt acagaaattc cccaccagtt
    58681 taccggaata tatgcatatc tctctttcca caattcctgc ggaaggcatt gtaaactgct
    58741 tggcaggcaa atctttatgt acaagattca ttacggtgtt ccatatcttc agtgcccttc
    58801 cgtattcctc ttttaccaaa gaaacgggag acactctgtt atcgtaaccg taccagaccg
    58861 cagccgagta gtacggagta tatccgcaaa accatttatc cagatagtcg gaagtggttc
    58921 cggtttttcc cgcaacggat atctgaccgt cgtttatata caccgaaccg tatgcagttc
    58981 cgttaatcca gtatgttgtg gaaaccccct ttaccacctc ttccagcata tcggtcataa
    59041 tagccgccgt ctgttccgag taagctaccg tataggtatt tgcccaacgc agaacggttt
    59101 caccggagtt attggctatc ctggttatgc tgtgagcatt catatgtatg cctttgttgg
    59161 caaatgtact gaatgcactt gccatctcca gagggctgac acctaccgcc aacccgccta
    59221 tggcagctga aactgtcgag tcgtcatata tatattcgct tctgtcgata cctaccctct
    59281 caaggtactc tacggaattg tctcttccca gataatatgc ccacactttg gcggctacaa
    59341 cgttaaccga tgccttaatg gcatttctta tggttgtcaa accctgataa tcaccggaat
    59401 cgtagttggt cggatatatg aggtcgggat tcttgacatc aaggtaacag ggagcatcgt
    59461 ctataacggt tgccgcggtt atgagtttaa ggtccaaggc gggaccgtat accgcaatcg
    59521 gtttgaaagt ggagcccggc tgtctgttca taccggtcgc cctgttgtat gtcctgcttc
    59581 cggttttctc cccgtttcct ccgtaaagac cggcaattcc tccggtatac tggtctatta
    59641 tgaccatggc tccctggggc ttttcaccgt atcttacggc attgatgttt atgattgttc
    59701 cgtcttcttt gtattcggcg aaataggaat tatcggtaaa tacactgtct aatgcatcct
    59761 ggaccttagg ctgtaaagtg gtatatatat gcagtccgtt attgtatatt attctttcgg
    59821 cctgtacctt ggatatggag tttttctcgg caagaaggtc tatggcctcg gttattacat
    59881 agtccacaaa atatgatgtg atggatgacg aaacgtaatc cactttctcc gcaaatacaa
    59941 tctcgtatgc cattgcatcg tcatactctt tttggtctat ataccctatt tccagcatct
    60001 tatccaacac gtcctcgcga cggtacatca gttttcgtat attctcttcg gtaccgtacg
    60061 ggttgtatgt gtagggagag ttgggtattg ccgcaagtac ggccgcttcc gcaaggttaa
    60121 tcttgtccag cgtcttgttg aaatacatct tggatgccga ctgataaccg taacagccgt
    60181 tacccatatt aacaaggttt gcgtacattg ttaaaatctg cttcttgtca tacttcattt
    60241 ccagcagtat ggcattgtat atctcctgta tcttccgctc gaatgtctgc tcaaaacgtc
    60301 ccgtaacatt cttgtacacc tgctgggtta tggtacttcc gccctgagct atgtcgcctc
    60361 tgttttctat gagtttcagc accgaactgc ctattcggat aaagtcaaaa cccgaatgcg
    60421 tgggaaatcg ctcatcttca atagcaataa tcgcatgctc cagtacaacc ggaagctgtt
    60481 tggaagttat aatttctctg ttgatattat cgcttcccgt cagcgtctca atgacatttc
    60541 cgtcaatatc atatataaaa gttgtaagcc cggtggtgat ggcaagatcc tcttcggtca
    60601 gaactttgga agtggaaatc catgcagtga tgattcccag tcccaggccc cctataagga
    60661 aagccgctat catgacaacc gcaataatta tctttaatgc agcccagaaa gtgcttaata
    60721 atgtactgaa aaaagaagga gtatactcca catactcaag atcggcagat tcctcctgct
    60781 cttcctcata tgaatctaac agttcatctt cattcattac attacctcat cgacataagc
    60841 gcacttatgc caaaaaaccg gcagactgca aatctgcccg catcattctt ttcttgtctt
    60901 gtcttcctta tcgctttcaa caacataaca cttgccggta aatttggcgc cctcatcgct
    60961 tatgaggctt aaagcatatg tgtctccgat gaaattggcg gtggaggtca ttttaatgta
    61021 tttctttgct ttcaatattc cctcggcttc gccgctcaat attatattgg ctcccgtaaa
    61081 ctgtcctttt gctttggccg atttggcaaa aacaatatca ccgtcggcga ttatatcacc
    61141 ttcaataacc ccttcgacgg ttaccgaacc ttttgtgttt atatctcctt ttatcgtgca
    61201 catggcacct ataagtacat caagtttttt ctgtctgatc attctttaca cctctgtcat
    61261 tcagctgaaa taaacagcgt cggatttatc gggacttcat ttacccttat ttcaaagtgc
    61321 agatgcggtc ccgtagccaa cccggtcttg ccgacatatg cgataagctg tcctttttcc
    61381 accgtatcgc ccgctttgac gagaatcttg gaacagtgag cgtacagagt ggaaatactt
    61441 tcactgtgtt ttattctcag ccaataaccg tatcccgatt tctcgtagtg gacagctact
    61501 accgtacccg tagcggaagc atatatctca tcgcccaccg ctcctcctat gtcaacaccg
    61561 gtatgcatct tgtattgatt taagatagga tgattcctca tgccgaattg tgaactgata
    61621 tgtttggatt tggtaggcca cagtgtggga atggaatcca gataattgca caaaacattg
    61681 gccgcttcgg aaaaatccac aagttccgtt ctggtactct catttgtcgc aagcaatatt
    61741 tccaatgcgg ccgcaacgga agctgtatct tctgcaaatt tggccacatt attagcagta
    61801 accgaagcgc tggcaagagt aatactgtcg gtatattttt ccgcaatcaa ttttaactgt
    61861 gaggataatt cttttatctg tatattaaga tcctgctgag cggtctccaa tgtggctatc
    61921 tcgttcaatt tgttatccag aaccatggcc tgttttccga cataggtact cagtgctatc
    61981 atctcactgt tgtaaccggc cataatactg tctatctcgg cattcatccg tttgttctcg
    62041 tttataaaat agaccaaaga aagactgagg caaacaatga ctgctatcat gaaatagaat
    62101 atcaatgaca acttcaatat aggtgatgtt acttttaaga ctctggaaga ttttccgccg
    62161 ttcggaatat acaccagcgt tccttcgcct tcaagctttg tccttccttt tctcatctgc
    62221 ctccccgttc tcaaattgat actaacacaa gcaggaactt ttttcaacag ttatgtgtcc
    62281 ctgcatcagt aaaattccat ccccgcggtt ctggaattat cctcgcacca ctgatgtgca
    62341 aaggaacctt ctttcacgta aattacggta agttttgaac agccgtaaaa aacctgctcc
    62401 ccgatattgt tgacacttgc ggggaaattg accgattcca gatttacaca atcccagaat
    62461 gccgaagctc ttatgctctc ggttccttcg ggaatttcca tcttcgtgat accggagcag
    62521 ttcttaaatg ccgtaggccc tatttccacc aatccttccg gaagaataat ctcggtcaga
    62581 taagtattat tacaaaaagc attcctgccc agatgtgtaa tgccggcggg aaccgtgtac
    62641 tgtttacctg tcttccgtgc cgggtatatg atgagctttg tcatatcctt atcgaataac
    62701 actccgtctt tagaaataaa atattcattt ccgacggtaa cagttatact tttcagatta
    62761 tcacaccctg taaaggcatt gtctcccaac tctttcagac ttttgggaat gttgaaagag
    62821 gttatggact tacagctgaa gaaagcatta tttcctatca cctccacgct atcgggcagc
    62881 acgatggaaa ccgcttcggt atttccccaa aaagcattgg aagttactcc ggtaacggga
    62941 agccctttta tttccgcggg taccgatatt ctgttgctct tgccgtggta cttggtaatg
    63001 tgaatctctt ccccgttaat tatatttata gaaaatagat tctccttggt gtccgcacag
    63061 gaaacggaaa atgtcgccac gagaaccagc acaagtacaa acattaccgc ttttacatat
    63121 cttttcatat cgcttcgcat gatacagttt actgctgttc acactcctcc tatctcacca
    63181 tgttaaatat atatatcaca aatgtgagca tattgtcatc aaaatccgga taaacgaaat
    63241 ttctatgaag ggaaattata tgaaaaaata gacttttttc tgctccgatg atataatccg
    63301 atattaataa tattctgaac ggggaggtgt cgacatgaga aacgcgatat tgattaagga
    63361 ctgcacactg aaaaactgtg agaccgataa agctttgaat ataagcttca aggaaaaact
    63421 ggaaattgcc agacaattgg aaaacattaa taccgatata atcgagacag gttttgcgga
    63481 cacgtcccag ggagctttta ttgcggtaaa aaccatcgca tccaatctga aaaagagtat
    63541 actatgctgt ccctctcagg cattcaaggc aagtatagat aagacatacg aggcaataaa
    63601 aggagcggtc agaccgcgca tacacatttc gcttccggta tcctcggtaa atatggaata
    63661 tcagttgcat atgaagaaaa accaggttgt ggagacggtc aggcaatcga tatcctatgc
    63721 aaaaaccctt ttcagcgatg tggagtttac ggcactggat gctaccagaa gcgatagaga
    63781 ttttctcatt gaaataatca atactgctat ggatgccggt gccactaccg taggggtgga
    63841 agattcggtg ggacacatgc tgcctgacga aatgcattcc tttttaaccg atctcataaa
    63901 atcaataggc agagataaca tagccttttc tttcagagca aaaaatgagc tgggaatggc
    63961 ggttgcaaac accttatcgg cgataaaggc gggagtgcac cagataacct gtgccataaa
    64021 cggtacggca aacgatgcca gtctggaaga gacggtttcg gcgatacaca cccggcagga
    64081 tgcaatgaat gtacaatgca atatgctgat agatcagctt aacagagctt cggtactgct
    64141 ctcgggaata accgatatta aaaacggcaa gataagaggc accgcaggca ccggtctgca
    64201 tatatccgaa tcggatcttc tggataagga aatggacttc tacagtttca gaacatttgt
    64261 ggaaaggctg ggatatactc tgaccgaaaa agacattgaa agaatatatg atatgttcaa
    64321 agaactctgt gccaagaagc aacaggtcta tacgatggat atagaggcta tcataggaag
    64381 agaagccgtt caggtaccca gaatttatcg tctggacagg tttgtaataa acagcggaaa
    64441 caacattact tccaccgctt ttatacgact gatgtattac gaaaaacccc ttgaaggagt
    64501 gggtatggga gacggcccaa tagatgcggc atttcatgtt atagagcaga taatgggcat
    64561 gaatatcgaa ctgttcgatt tcaatttaaa atcggtaacc gaaggaaaag atgccttggg
    64621 tgccgctacc gtaaagatac gctataacgg aaagatacat gtcggaagag gtttatcgac
    64681 cgacgtgata gaatcctcca tattggcata tataaatgcg gtcaataaga tgctttttga
    64741 ggaaggtata caatgaagta tgcgttttac acggcgaacc ggaataattt aacatggaaa
    64801 gaagcaatta ccacggcaaa agacttagga ttctccggta tagaaatagg ggccggtttg
    64861 cttgatgcgc agccttttac ttcacagtat attttaagaa ccgccgaaca gctggcgaat
    64921 cttaatctgg aaatatcctg cttttcctct tcttgcagtc ttaacgatag ggaaaaaaga
    64981 aaagaaaaca tacagcaact gagagagctc atagatattg cagccaggat gggtgcttca
    65041 tttgtacgac tgctgcctga tgatgatata atgcccaacg acagagctac cgaccaagcg
    65101 gttatctcgg caatatcggc aatattgcct tatgccgaaa aaaaacatgt cattctgctt
    65161 atcgatacaa taggcatata ctgcgatacc gacagattaa gaaaagtact ggaacatttt
    65221 gcatcggaca accttgccgc attatggaat gtccatcatc cttacagatt tcacaacgaa
    65281 tcggcggaga aaaccgttca gaacttgggt gcatacataa agtatgtcca catgaaagac
    65341 tcgttgatga atgacggtgc agtgaaattc acacttatgg gtgaaggcga tttacccgtg
    65401 gatgaaataa tccgggcact gaaatcggtt aattatgacg gatatatagc ctatgaatgg
    65461 ataaccgatc aggattccga tctggacgaa gcggatatag tattccccca cttcatgaac
    65521 tatataacat ccaaatttga ggaaaataca aaagcaaata aactctacta taacaaagcg
    65581 aaaaccggcc gtttcgtgtg gaaaaaagaa cagctcatag actgcacatt cccgcaactt
    65641 ttagacagga tggtggaaga atttcccgac cagtatgctt tccgatatac cactctgaac
    65701 tataccagaa cctattcgca atttcgggat gatgtggaca cattcgcccg ttcgctcata
    65761 gcattaggtg ttaaaaaagg cgatcatgta gccgtgtggg caaccaacat accccaatgg
    65821 tacataactt tctgggcaac caccaaaata ggggcggtgc tggttacggt aaatactgca
    65881 tacaagatac atgaagcgga atatctgttc cggcaatcgg atacgcatac aatagtaatg
    65941 atagaaggac ataaggattc gcattacgca aagatagtaa aagaactctg tcccgaactg
    66001 gaaacttccc ttcccggaca acctttacgt ataaagagac ttccctttct gaggaatata
    66061 ataacggtgg acttttccat gcaaggatgc ataacatggg aagatgctat ggatatgcat
    66121 gtaaaaaccc ccgtagagga agtatacaga agagccaatt ccgtaggtaa acacgatgtc
    66181 tgcaatatgc agtacacgtc gggtaccacg ggattcccca aaggcgtaat gctgacacac
    66241 tacaacatag taaacaacgg taaaagtata ggagaccgta tggatttatc cacagccgac
    66301 agaatgatga tacaggttcc catgttccac tgtttcggaa tggtcttggc aatgacagcc
    66361 tgcgttactc acggaagtac aatgtcgcct cttccctact tctcaccccg gccggcactc
    66421 aattgtatca atctggaaaa gataacctgt ttccatggag ttcccacaat gttcatagcc
    66481 atgctggagc atgaggactt caagaaaacc gacttttcac acatgcgtac cggcataatg
    66541 gcgggaagcc cctgtcctat caaggtaatg gaagatgtgc aaaacaagat gaatatgacc
    66601 gaaattacca tagtctacgg tcagaccgag gcttctccag gctgcaccca gagttccacc
    66661 gacgattcca ttgagttaag ggtaaatacc gtaggtaaag cgcttcccgg aatggaatgt
    66721 aagattgtag atcccgaaac atatgaagac ctgcccgacg gtaccgacgg agagtttgta
    66781 gtaagaggat ataatataat gaagggctac tataaaatgc ccaatgcaac cgctgcagca
    66841 atagataaag acggatggct gcataccgga gatctggcaa gaagaaacaa cggctactat
    66901 aaaataaccg gcagattgaa ggatatgata ataaggggcg gagaaaacat atatcccaag
    66961 gaaatagaag atttcatata tacccatccc aaagtaaagg acgtacaggt tattggggtt
    67021 cccgacgaag gctacggtga agaaataatg gcagttgtaa tattaaaaga caatgaaacc
    67081 atgaccgaaa aagacttaaa agattatgtc cgcacccata tggctaaaca caaaactccc
    67141 aggtatgtgg aatttgtaga ggaatttccc atgaatgccg ccggcaagat actgaagtat
    67201 aagatgaggg aaaatgccat agaaaaactc aaactccaga aagcaaacgg catagtaacg
    67261 gcataaccgg aaaacaaata cattaaatcg atatgatata attagactgg aaaagcagca
    67321 taaacttttt tatcaaacct tcaggagaga ataaaatgaa agtgcttatc ataggagcaa
    67381 ccggcttatt gggaagtgaa gccgccaggg aactgataaa aagaggacac agcgtaacag
    67441 gtatggcttt gccgcccgta ccccaaggag cggatatacc tgatcagatg caactgatac
    67501 ttaaagattg catgtctctt aatattgacg aactggcctc aattatgaaa cagtgtaacg
    67561 gactggtatt tgccgcaggc gtggatgaaa gagtggaagg tccccctcct atatatgata
    67621 tgttctacag atacaatata gtgccgttac agaaacttct gcctcttgcc aaagagtgcg
    67681 gaataaagca gaccgtaata tgcggttcct acttcacata tttcgaccgc acattccccg
    67741 aatgggaact gtacaagcac catccgtata taaggagcag agcggatcag gcaaaaatag
    67801 ctctggatta tgccgatgag aactttgccg tatccatttt agaactgccc tatatattcg
    67861 gtgcccagaa aggcagaaaa ccggtctgga cattcttagt ggaaatgata agaaaaatgc
    67921 cggtagtgac catgtacccc aaaggcggta ccgctatgat taccgcaaga caggtaggtc
    67981 aatgcatagc ttccgctctg gaaataggaa aaggcggtca gctcttccct gtctgtacat
    68041 ataacatgac atggaaggaa atgcttgcaa tgttccatga gcatatggga atgcccaaca
    68101 gaaagatagt aaccgtaccc acctgctttt tcaaagcaag tgtaaagaaa cttgcaaaaa
    68161 aacaaaaggc tgcaggacat gagggcggat tggatatggt taaattcgta aaagtaatga
    68221 catatgaagc ttacatgagc gatttcaatg caaaaaccat attaagggct cgaccggatg
    68281 atataaacaa ggctataggc gaatcggtac ggttatgctt agacatattg gacagcaata
    68341 cccaagttgt cgacatgaag ggcgaataat tgaagcaagt agagctgaat ataattgata
    68401 tatcatacga aggaaaagga atagcaagga aagataacct tgtctatttt acagataatg
    68461 ctattgacgg agaaacggta acagcagaag cgtatgaaac aaaacctaaa tatgtaaaag
    68521 caaaaacaat acaaatactc aaaccgtcaa aatacagaac cgaaccgaaa tgtgctgtat
    68581 acaataagtg cggaggttgc agcttaaggc atatgtctta tgaaagacag cttaaactta
    68641 agaaagatac agtaatagcc aacttgaaaa gaatagcaca catcaacata gcagacataa
    68701 caatacacca aaaccctaaa acagattatt atcgcaataa ggcggaatat aagattaaga
    68761 acggaaagat aggattttat gaggataaaa gccataatat cgttacgcat gaggagtgcc
    68821 tgcttcttaa agaagagatt aacaatataa agaaaagcat agagaaacat attacaaaag
    68881 acatgactgc gttaacaata aaagaggctg atgacgggta tatggttata gcggaaagcg
    68941 ataaaactct acccgacaaa ctgattaata ctctgaaaga aaacaataaa gtcatatcgg
    69001 tatatcaaag gaaacaaaac ctaaatatac aaatctacgg aaaacaatac ataaccgaca
    69061 gcttatgcgg tctgaaattt caaatatcac cggacagttt ctatcaggta aacaaaatgc
    69121 agacagaaag actgtataac atagccaagc actatctgaa agcagcagat aaaaataata
    69181 acttaaaaga taaaacagtt ttagacctgt actgcggtat aggtacaata agtcttttta
    69241 ttgcagacag cgtaaagtat gtgataggtg tggaaatagt gaaagaagcg atagataacg
    69301 ctaaaagcaa tgcaaaactt aatgcgatag aaaactgtca atttattcac gccgatgtaa
    69361 atgatgcaat cttgcagatt gaatatgaca tacttatcgc ggatcctccg agaaaaggat
    69421 tggatgattc ggttatacag gctatcagac aaaagaaacc ggaaaacata atatacatat
    69481 cctgtaacag ctctacactt gcaagagata taggtcaatt aagcgacctc tactccctgt
    69541 ccgaaataga agctgtggat atgttcagcc atacaagtca cgttgagtgc gtggtcttga
    69601 tgacaagaaa gtgaatcagg atagtcatgg aaatgcaggt aaagaaagca attctgtcaa
    69661 ctcgacttaa actattgatg tgtgtagtaa gcggctaata atcccatggg tttaaaattc
    69721 ttgtataaac acaattgaca agtctggatt cttatttttt ttttacgtta ctcttttctg
    69781 ccggatgttc ttcctgcgta agtgagcagt ctaacaaaaa tcagcccgca accatatcca
    69841 caactgaaaa taatacgtca gaagatatta atcagaataa tatagaaaac agtgatgtac
    69901 ctcttttggc acagccaatt gaagaacagg gacagctttt tatgggcttg aacttggatg
    69961 gaattggaaa ttatgatgat gaggcatatg tgagattata tgtttgggat cataatggat
    70021 ttggtagata tgatgcttcg caacttgtga tacgacagtc atacaatccc tcttgacaat
    70081 gaatgaggag aagatatata attaaattat cattaaacaa ccggttagtt taattggagg
    70141 aatataattt gaaaaaaaac tctgatggtg aagcaagaga acgaataata aaagcggcta
    70201 tacaactgtt ctctcaaaaa ggttatga
//
LOCUS       DAOSOM010000007        61019 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_538_length_61019_cov_7.826553, whole genome shotgun sequence.
ACCESSION   DAOSOM010000007 DAOSOM010000000
VERSION     DAOSOM010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 61019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 61019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61019
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_538_length_61019_cov_7.826553"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="PLF14_02945"
     CDS             <1..1083
                     /locus_tag="PLF14_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70287.1"
                     /translation="YNISITGLNLLCNGHNSKEGNLIVGLRGQVCFFYVKDLVIRDLL
                     CLDLMEHSFCIHICTFENLLIENVHIEGLKDGIHIDKGSKFTIRHGVFKTFDDPIALN
                     AHDYTSCAPELGWIEDGIIEDCYDLDQEKTTGYFCRLLAGSWLDWFKGMKVQQSDTVV
                     YNNKIYRVNMQPDGKIYTSNTAPNHSEGTKEYDGIRWTMLQDREIVYNCGCRNIHFRN
                     IFLKKKRHMAFALTFDRNEYSRGYYPNSIPPVQEDITFERIYVQNEIIDLLGIRTPVN
                     TIKLTDSVIGNNRIYLRYLEEEGITYIPAHVLISNTTFKGDGKQSLVYAEKGRKATLK
                     IKGSIKENASYEPLIEGDIEILKKDI"
     gene            complement(1234..2508)
                     /locus_tag="PLF14_02950"
     CDS             complement(1234..2508)
                     /locus_tag="PLF14_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOT70288.1"
                     /translation="MDKELYKPRLVDKQVKLELEAFGAVCIEGAKWCGKTWTSGYHSK
                     SAIYLGDPSRNFQNRELAKLEPTTVLEGEVPRLIDEWQEVPSIWDAVRHEVDRRGKKG
                     QFILTGSSTPKQKGILHSGAGRISTIRMRPMSLFESNDSSGQVSLTSLFDGTFKAVAT
                     GNIDLETLIKLTIRGGWPESLGLDYEKFRLLPKEYIKSIINHDIYRLEGINRSSAKMQ
                     LLLQSLARNESTTASIATLKKDISDFDNDTLDNDTISTYLSTFERLFLIENQKAFSSN
                     IRSSTRIKQSEKRHFVDPSLAIAILEASYEDLLNDLHTFGFMFEALCVRDLRIYAESL
                     EAQLYHYQDYKEREIDAVVKLRDGRFGAFEIKLGADAIEKAAQSLIKIRDDLKAEGAR
                     IPDILVVICGLSNFAYQREDGVYVVPITALRP"
     gene            complement(2806..3129)
                     /locus_tag="PLF14_02955"
     CDS             complement(2806..3129)
                     /locus_tag="PLF14_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70289.1"
                     /translation="MTKVMIDPGICNLVTSVEASSDDGMEVTLKVKSGCKSINDMFKD
                     LGDTFDAYELCLGKPGSGPLYDYAKEHLPGHCGCPAIAGIIKAAEVECNLALPKDAKI
                     TFVKQ"
     gene            3294..5090
                     /locus_tag="PLF14_02960"
     CDS             3294..5090
                     /locus_tag="PLF14_02960"
                     /inference="COORDINATES: protein motif:HMM:NF014852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HOT70290.1"
                     /translation="MGRYTQKISLRLIPLPKELSCNDIKKVAFNDIRIDVIYPEDALL
                     RSAKRKLVSFFGNSLYGSFRIRIVDPMPETLNVLSHFPNSDQAYRIWSDGSINISALT
                     SKGIMNGVRTLLQAVSKEDGMFNMPLLFIYDYPDIPLRGQRSETAVRDIAYMASLKLN
                     SADYMSINSIDEEKRLKENCINEGIVLNSNISYSGCITDENLCVLSENADTVIFDWMT
                     YGGKCIFPRTAVDFIKENCEKFVENKTRKVVCYTPVSDIYQKFNITAMAEWLWNAKSR
                     DAESFMRAYATVNQMDSDKFVKWCTYNMTPARTLAENGFIERLMSNRTVTVKGKEYLD
                     YCGFDDFIENAIIDIDKYIRMADRAVRAAKQTGYEEGETESRCITAALRCAGAQQKFF
                     TLYRMKKRNPEEEQQMVLMYEMMHNNSRSLYYALSDWKNVVDKENLHFHTGITCTAVA
                     CMRMADIMKKILEEEDIKHIEHHSLSYHEIGGWDVSVFNKEGIAELTYDIAKYADKEG
                     RYHICFDYTDGKSDISVKSISVLYNNVEKAHIIYDNTSSLKRLNNDEPWYEVFIDIEC
                     TGRAEEKILKVTLEKATDSKDKCNGIVGIRKI"
     gene            complement(5091..6119)
                     /locus_tag="PLF14_02965"
     CDS             complement(5091..6119)
                     /locus_tag="PLF14_02965"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT70291.1"
                     /translation="MNSRERVHAAIHFQPVDKAPLQYNYTPVGYFEHGEKLNELYASV
                     PDDFGPFQKKEIPVLTKDQLDENGNYHEFKKDEWGTVWEYRIYGIAGIPHIYPLEDMS
                     NLKNYKMPEQSEIGSEQFLQDKRDAVLHKKEYYLSRFAGTMIEKLKALCPDENIYCDM
                     AEDTAEINMLADMIVEYYEKKIQYLLAIDVDGFYFGDDYGTERGMILSPALWRRFFKP
                     RWNRLFEPIRAAGKNIHFHSCGYVLPILEDLKELGVHSIWPQLPAYDMEKFAKYCRSL
                     RLAVAIHTDRANVMTFGTPSEVRDLVKREYETFRMYEGGSWFYVECDNGFPFENMEML
                     VRTIAEYR"
     gene            complement(6165..7910)
                     /locus_tag="PLF14_02970"
     CDS             complement(6165..7910)
                     /locus_tag="PLF14_02970"
                     /inference="COORDINATES: protein motif:HMM:NF019313.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase fold protein"
                     /protein_id="HOT70292.1"
                     /translation="MSRLGYTDKPVEKLTEDSFGIGTYINGLCQFIVSCDTPMTISIQ
                     GDWGSGKTSMMNMIKREIEGEVYPIWFNTWQYSQFNMGNDLCLSFLENLITKLQSKAE
                     TATSKILETSLKILKIGAKTAISSVGLDGAAIVNVFDKQDSDIAKAVNDLKDAFQESV
                     NNKLKDNKKDRVVIFVDDLDRLNPGKAVELLEVLKVFLDCENCVFILAVDYSVVTAGV
                     RQKFGNLVDEDKGKSFFDKIIQLPFKMPVAQYDINKYIYDMLKKMNMKANSRIVQDYK
                     QLIRNSIGFNPRSIKRLFNTYQLLNLISDSKKTSVREDLHQRLLFAIICMQMTYEHLY
                     AFIVTSAKNDELTGKGLRTLADPEVVRNYKGLPEELRLIGKDGKIDEDELIRMCAFMK
                     TFVSIVDIDKDKLLSDEELNDMKEILFFSSVTSIMEEAKTGTGMEADQKIRARNRAII
                     DDVDANLTNLIGDFEIWQPLKSESDTKMSDVRCSINLITVSGIPFKYEFSISSKTLQD
                     ICLFIKFTCVQENLERLKEFFGPNPLDNEIPPVINDKGYEYVCMNIPEDKAVEVITEK
                     VKEAHGKLKTKGYIV"
     gene            complement(8110..9156)
                     /locus_tag="PLF14_02975"
     CDS             complement(8110..9156)
                     /locus_tag="PLF14_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase/BAAT C-terminal
                     domain-containing protein"
                     /protein_id="HOT70293.1"
                     /translation="MKIFFGILKTIAIILVIIITSFMLIFGIRTYNGIKYKSYKHDIG
                     EALSPTDLSQYNTNIEGVEVSRITGEYMNGFRLKPLNKTYKGVIITFGGSEGSPSYHS
                     AEAFARAGFETLALFFFGMENQKEELVRIPLDYFEEVLAYIDKNIEDNEIITVFGLSK
                     GAELALNLATLYPEIDNVILSAPTAWNYMGLSKNYSREMMSSWTYKGEELPYIDMTKG
                     SPEAATNLILDFIFNRPIDYRPGYESAANNDPGSEAARIKVENCDANILIFAGAMDKM
                     WQSELSARVIKEHKPENTDIYIFDDAGHIFFLGPYVYVNGMMLEMGGSLETNTKAGEE
                     SNVILVNKIKEWHK"
     gene            complement(9440..10768)
                     /gene="xylA"
                     /locus_tag="PLF14_02980"
     CDS             complement(9440..10768)
                     /gene="xylA"
                     /locus_tag="PLF14_02980"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HOT70294.1"
                     /translation="MKNYLKTIPKIKFKGSKSTDPFSFRFYNPNEVIHGKSMKEHLRF
                     AMSYWHTLCGTGVDMFGSGTADKSFSKKEGTLAHAKAKADAGFLLMSKLGIGFFCFHD
                     VDIAPAGETLAETHTNLDIMADYIKEKMDSTGIKCLWGTANLFGHPRFMHGAATAPNA
                     DVFAYAAASIKKAIDITKKFDGQGYVFWGGREGYETLLNTDMKLELDNLARMMHMAVD
                     YKNKIKFNGPFYIEPKPKEPTKHQYDFDAASTISFLRKYGLEKHFKLNIEANHATLAG
                     HTFEHELRTAAINDMFGSIDANQGDMLLGWDTDQFPTDVYNAVYCMLEVIRAGGFTTG
                     GLNFDAKQRRGSYTPEDTVIAYIAGMDTFALGLKIAYAIIDDGRIDDFVKERYKSYTH
                     GIGADIVNGKTDLEKLTRYALSNGEITTNTSGRQEMLESIINSIMFGNRF"
     gene            complement(10892..11995)
                     /locus_tag="PLF14_02985"
     CDS             complement(10892..11995)
                     /locus_tag="PLF14_02985"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HOT70295.1"
                     /translation="MRKNFHVLVKPASSLCNMNCKYCFYDDVSNKRKIKSYGIMSQDT
                     SHKVIDRALQACPEGEITFSFQGGEPTLATLDFFLDFTEYAQQAKTEKVKYMYSIQTN
                     GLLIDKAWCRLFKKHDFLVGVSLDGLKEAHDTLRKDYFQKDTFNRVMNGINLLKKYEV
                     PFNILTVATKYNVRHPQKLWNIYEKNGFDYIQLIPCLSSLDNTNCNQPYALEPKAYAD
                     FLKTFFDIWLQKYLQGKYVSIRVFDNLVKMCAGYPPEQCGMTGFCQAQFVTEADGSVY
                     PCDFYVLDDFNGGNVHEQDFDSILNSDNFKKFLKDNSSYNLICNNCEVKDYCNGGCRR
                     YRYFYFQTNDYCPQKDFLTHALPALKKIAASLR"
     gene            complement(12118..12193)
                     /locus_tag="PLF14_02990"
     tRNA            complement(12118..12193)
                     /locus_tag="PLF14_02990"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12158..12160),aa:Thr,seq:tgt)
     gene            complement(12198..12273)
                     /locus_tag="PLF14_02995"
     tRNA            complement(12198..12273)
                     /locus_tag="PLF14_02995"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12238..12240),aa:Val,seq:tac)
     gene            complement(12371..13597)
                     /locus_tag="PLF14_03000"
     CDS             complement(12371..13597)
                     /locus_tag="PLF14_03000"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015852184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOT70296.1"
                     /translation="MKSLKELYRIGRGPSSSHTMGPDRAARRFGEEHPDAERFEVTLY
                     GSLAKTGLGHMTDKAVKEALSDKPVKVHIDTVTEDLPHPNTMDFVAIKNNEVADKWRV
                     MSIGGGAIRIEGKPYMDSPDFYKEKNFTEIKKYCEKHHLKLYEYVEQCEGPEIYDYLD
                     DVWHHMKLSIKEGLNATGILHGGLKLQRKARYLYRQRHMDESPSTRENRIVCSYAFAA
                     SEQNAGGGIVVTAPTCGSCGVLPAVLKYMQEKNGYSNRDILRALAAAGVIGNVIKTNA
                     SISGAECGCQAEIGTACSMASAGLAELFGMDLDQIEYAAEVAMEHHLGLTCDPIMGLV
                     QIPCIERNAVAAMRAINSVSLANFLTHTRKISFDMVVDTMYETGRDLFSKYRETSEGG
                     LAIKYSEKKGNKLYTT"
     gene            13920..14744
                     /locus_tag="PLF14_03005"
     CDS             13920..14744
                     /locus_tag="PLF14_03005"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT70297.1"
                     /translation="MKNKSVIKTINIIVPIFILGLIIFNSTIEALVLAVFYFIYRIII
                     GLSKIYSYIAIKYYVKGNLKKARKYYKKAYEVKVKEPPIVASYAYLLILLGEYDQAEQ
                     VLQVLESMKPVGKAATSLMLCNAVLMWKRDKNLIAALAKVESMDETMRNQWYYNVYAK
                     LCIFSGNIGKAKEAALKGYEYLESNPVAVENLLIIHCINEEYDKALSAANKLINNKKF
                     RASSQDAYYYSALAYEKNGDKETAKKLYLKALRYENTTMTYVDKQKVLDKAGEYMK"
     gene            14741..15649
                     /locus_tag="PLF14_03010"
     CDS             14741..15649
                     /locus_tag="PLF14_03010"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOT70298.1"
                     /translation="MKEKLKVAWPKIADYIVMTVGVVISAAAVNLFFVPYKIHSGGVS
                     GIATVLYYLFNNKVPVGVLVALLNLPLFLIGYRIIGKRFMIRTLYATVMYTVAVDVTA
                     KWLSEVSKKITIESGNIPDLMLFCFFGGALMGLGLGIVFTRNACTGGSDLFAEIMRRK
                     GIHYSIGTLMFMFDGVSVLMSLIVFKNLLLVLYSVIAIFVSMKVIDLILDGANVAKAV
                     YIMSDKSEEIADAIINDIGRGVTGFAGRGMYYRQSRETLFCVVKNRQLPALKKLVKEL
                     DSKAFLIVTTAHQVLGEGFGAFDKEL"
     gene            15727..16569
                     /locus_tag="PLF14_03015"
     CDS             15727..16569
                     /locus_tag="PLF14_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOT70299.1"
                     /translation="MEQELLLKLKRKAADVRKGIIEAVFSAGSGHPGGSLSCADILTV
                     LYFKHMNVNPSKPKDPDRDRFVLSKGHAAPVLYSVLAEKGYIPKEDLFTFRQADSYLE
                     GHPSLKDVPGVDMTTGSLGQGICAACGMAQMGKLDKKDYRVYAVLGDGELQEGQVWEA
                     MMYAAHYKLDNLTAFLDHNGLQIDGDIKEVMSPEPVVAKFEAFGWSVILIDGHDMDQI
                     DKAIKKAKRTKGKPTMIVAETIKGKGVSYMENKYEWHGKAPNQEQRDIAIAELDAYIE
                     SLGV"
     gene            16576..17511
                     /locus_tag="PLF14_03020"
     CDS             16576..17511
                     /locus_tag="PLF14_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HOT70300.1"
                     /translation="MKKATRAAYGEALAEYGSDERIIVLDADLSKSTMTAKFKEVYPE
                     RFFNTGIAEANMISSAAGIATCGKIVFASSFAIFAAVRAAEQIRNSVCYPRLNVKIAA
                     THAGISVGEDGATHQAIEDIAVMRALPNLTVIQPCDGRETKQAVKAIIDYDGPVYLRL
                     GRLPVEDIYDNSYEFTWGKGVQLKDGCDVTIIATGLMVGSALEAYEILKAQGILARVI
                     NIHTIKPIDREIIIKAAKETGAIVTAEEHTIIGGLGSAVCEVLSEEYPVPVKRIGIED
                     KFGKSGKPNELLKLYGLTPEDIVIKAKNVMTLKRK"
     gene            17508..18302
                     /locus_tag="PLF14_03025"
     CDS             17508..18302
                     /locus_tag="PLF14_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOT70301.1"
                     /translation="MIFETHAHLLAEQFDQDRNEVIQRALENGILAVLNVSDDMKSSK
                     KAVMLAQKHERVFGSVGVHPHNGADFRDQDVSILKELTSDEKILAIGEIGLDYYYDFC
                     DRTVQREVFVKQLDLAKQLDLPVIIHNREAHKDTIDILSSFGKDIKGVMHCYSGSAES
                     AKILLNLGMYISFTGVITFKNAKRAVEALEVIPLDRLLLETDCPYMSPEPLRGTRNEP
                     KNLVYIARKMAEVKKISYERLLEQLWENSFRLFKKAEKYKQTLETT"
     gene            18299..19159
                     /gene="rsmA"
                     /locus_tag="PLF14_03030"
     CDS             18299..19159
                     /gene="rsmA"
                     /locus_tag="PLF14_03030"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOT70302.1"
                     /translation="MKNTDNIIPLPDYVRAKKALGQNFLTDKSIALKIIEALNLDKED
                     IVIEVGAGTGAITALIAPLCKKLYAVETDKDLIKPLAAVLAKYDNVEIINKDILKTDI
                     NGIIDKECRYKTVANLPYYITTPVISKFVEQENPPDVMVFMMQKEVADRLCASEGSRI
                     YGSFTIYVGYRYEPSFVVNVPPQSFIPAPEVDSTVLKFIKREKPPVVVPSEEFFFSVV
                     RTAFSQRRKKLSNCIAAMPMFDISKNETEDILIQMGIRADARAEALSMEQFAELSWRL
                     YDRNTDNVNK"
     gene            19341..21686
                     /locus_tag="PLF14_03035"
     CDS             19341..21686
                     /locus_tag="PLF14_03035"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008816017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HOT70303.1"
                     /translation="MITKIIKRDGREESFSSFRITRAIFLAAEAVAKEENTKADFTIA
                     EQLTEKVVQLLNRRFIETIPTVEDVQDAVIKVLIEEGHAKTSEKYILYREARSKARSM
                     KTRLMKAIEEITFHDANQSNLKRENANIDGNTAMGTMLQYGSAVSKEFCKTNLLDPKH
                     TKMHEEGDIHIHDMDFYNMGTLTCCQIDLSRLFKHGFSTGHGHLREPQDIASYTALAA
                     IAIQSNQNDQHGGQSIPAFDYYLADGVAKTFRRTYRSNTIKAMKIIYGSVVPDCEDKI
                     KNFEKEVGLYAKIEMHSEYLKKFNDFLVKECKVKTKDLDKINKFAYSESLAETDKRTF
                     QAMEGIVHNLNTMHSRAGAQVPFSSINFGTDISPEGRMVTKNLLLAQDAGLGNGETPI
                     FPILIFKVKEGVNYNPEDPNYDLFKLACRVSAKRLFPNFSFIDAPFNISYYKPGNPDT
                     EVTYMGCRTRVLGNINGDEVVTRRGNISFTSINLPRLGLKHGVVLKGKFDEAGFFKEL
                     DEKMETVKQQLLERLVVQGSKKVKNFPFLMGQHLWMGSENLGWEDTLETVVKHGTLTI
                     GFIGLAECLKALIGEHHGESEKAQELGLKIIGHMRKRCDEFMEENNLNFSLIATPAEG
                     LSGRFTALDKKIYGTIEGITDREYYTNSFHIPVYYKISAYDKLQKEAPYHALTNAGHI
                     SYVEIDGDPLKNLEAFEDIIRYMKECNIGYGSINHPVDRDSICGYTGVIQNSVCPNCK
                     REEKNGPKFERIRRITGYLVGTLDNFNDSKAAEERDRVKHA"
     gene            21679..22197
                     /gene="nrdG"
                     /locus_tag="PLF14_03040"
     CDS             21679..22197
                     /gene="nrdG"
                     /locus_tag="PLF14_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOT70304.1"
                     /translation="MHKIRLSGKCTRDSTVDGPGLRIVIWTQGCLHNCKGCHNPHTHD
                     LNGGFTEDIDKVIEFINQSKIQRGITLSGGEPFLQPEALIPIVKAAIEKGLNVWAFSG
                     YTYEQLLKDENKKALLDYVDVLVDGPFILEQKDERLTYKGSKNQRIIDVKQSYAQNKV
                     VLSRYDEVNADF"
     gene            22278..22976
                     /locus_tag="PLF14_03045"
     CDS             22278..22976
                     /locus_tag="PLF14_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT70305.1"
                     /translation="MYNILICDDDYDIVSALKIYLSGDNYNIFTAYNGKKAIEIIKEH
                     KIHLVLLDIMMPEVDGITAMVEIRKISNVPIILLTAKSEDNDKILGLNLGADDYITKP
                     FNPVEVLARVKSQLRRYMHLGSQTKSQAVIRIGDIELDDDAKTVTVSGEEVSLTPIEF
                     DILRLLMKNPGKVYSTKHIYSEVWDDEPYGAENTVAVHVRHIREKIEINPAEPRYFKV
                     VWGQGYKMERQNQQ"
     gene            22973..24841
                     /locus_tag="PLF14_03050"
     CDS             22973..24841
                     /locus_tag="PLF14_03050"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT70306.1"
                     /translation="MKNIKYGLLMKIMAYILITISALAFAVSVSASVYIKGSDIDRNP
                     GMAFFESEICKKITHDYATEVYNGYFKEYMLTYMDWQKLEDYKQYYSKENTNFFFAIT
                     DKWGNHVLSSYTDTYGYRNTFVMEQDGYTVTVFVRTDITAEDDYLFPYKLYTFAHEVR
                     HLLIPIASVSLVVTVLLFIYLMCSAAHKKGSDALEPSFIDKIPLDLLLVVFAGLIILA
                     YLLFSELVTALYSMYMFEELIMVLSFGFAFVFILFMILAASIAARIKLKTLFKNTLIY
                     IVTRAVFRFIWKILGKLPLLWKLLLFIGAVSLIELVVIVVFTAKGGAAPAVIWFLLRM
                     GVTAFLCFITLNMIKIKEGGEEISRGNFQYKIDNKHMLTDFKRHADNLNNISEGMSDA
                     IKEKMKSERFKSDLITNVSHDIKTPLTSIINYVDLLDRQNIDDSTSREYIEVLKKQSA
                     RLKKLTEDLIEASKATSGVIKANMEKLNLKEIMNQVSGEYSAKFTENNIEPVINDFGE
                     DIYIWADGRLIWRVMDNMMGNICKHSHPGTRAYITVEQQDNRIKICFKNVSKEKLNIT
                     AEELMERFVRGDSSRTSEGSGLGLSIAKSLTEIMQGTMQLEIEGDLFKACLIFNKG"
     gene            24878..26731
                     /locus_tag="PLF14_03055"
     CDS             24878..26731
                     /locus_tag="PLF14_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70307.1"
                     /translation="MDNNKKRSAVLSFALIIVIIATALISASCNNPVFIRNSFNATTF
                     NSDIVIKRTDGQEPLNMPYSYSKALLDNRLLFEEEIIGFNISSVKYEFSDTGMNMYAN
                     RDVFVNEDSREVKAVMDSVKYCKGITTLNGITSDKEDSKITVYEGYSANLLTESLRNY
                     VIIPSTLSRHINKELPDNEKILFIVNPGTNGLAYFTIIGEYTTKNEKDTLYLSYEGFA
                     NMARGGQEDISEHVECMEIDVNEQADLTNFSFFLSAYFADINAPDQYENRINRLNEPY
                     PYMFVNSVNIKPISFTEDTNFKKNIITVSRIDKEEKLLMSYLYSDALIKDYYEYSRYI
                     TDINISTGIKGVNWRDYLTPADYKEYGLSVEPFDEFGNQWSDYRNKSGKNCADYFYQQ
                     AVTSISEIKKMKSNCKITFYSNYTNRDLVVQRKEDYKEAAFMGEIMMAPPIKGYAIIP
                     VTMYEAARSRRNTDHHIIEMAEISAAYLARSALRVGFKVIGYYEVSEEQKVEVPDRKA
                     EEIMVEFYDHTGNLIKMPVVEKPEYISDGYDVIYLSYIGYNDRYERELFRNEYIESIT
                     IETRSDSDIAPLIRYLEQYFAPATDVKKYEKEKNVLGLDYEYCYTIKEKAD"
     gene            26879..27469
                     /locus_tag="PLF14_03060"
     CDS             26879..27469
                     /locus_tag="PLF14_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009003554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HOT70308.1"
                     /translation="MIIDKINKKMKNNNYILSTEQLTDIGVSKTTLSNYVREGLLDRC
                     GHGYYTVPDSIEDDMYLLMLRSKHIVFSHETALFLNGLSERTPFYHSVTIPSNASLPA
                     SVKQKCNCYYIRPELYTLGLYEKVNTFGNIVRCYDPERTICDILRSRSRLDEETVLSA
                     IKNYASFGGKDLNKLGNFAEAFRVSKSLRIYLDVLL"
     gene            27523..28068
                     /locus_tag="PLF14_03065"
     CDS             27523..28068
                     /locus_tag="PLF14_03065"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOT70309.1"
                     /translation="MNYIILDLEATCEQNNRDYPNETIEIGAVKIDENFNIVSTYNEF
                     IKPEINPILTDFCKQLTSITQEDIDNAKTFPDVIRHFKNWIGVETEEYILCSWGFYDK
                     KQFTRDCELHGLDSSWTNRHISLKHQFGTIRNEKPCGMEKALNKLNIPLEGTHHRGID
                     DAKNIAKIFLMYKDKWVFTYE"
     gene            28234..29250
                     /locus_tag="PLF14_03070"
     CDS             28234..29250
                     /locus_tag="PLF14_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HOT70310.1"
                     /translation="MPFNIIRQDITKMKVDAVVNAANTRLKMGGGVCGAIFRAAGAEA
                     LQAACDKLAPIETGKAVITPGFDLPAKFIIHTAGPIYNMQTAKESERLLRSSYTESLR
                     LAVEYKCESIAFPLISGGIYGYPKDQALQVATSAIRDFLKEQDIDIYLVLFDKSAFVI
                     GRELLGDVKSYIDEHYVERHEVKRERFLGAESRTMRKAKASINRCFGPMYESADAATT
                     LDDVIGNLDEPFSDMLLRLIDSKGMTDVEVYKRANLDRRLFSKIRSGKGYMPSKRTAI
                     AIAVALKLSLEETEALLKRAGYALSHAVKFDVIVEYFIINGKYDIFQINEVLFEYDQP
                     LLGN"
     gene            29341..29994
                     /locus_tag="PLF14_03075"
     CDS             29341..29994
                     /locus_tag="PLF14_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOT70311.1"
                     /translation="MKKGLTELVFILDKSGSMSGLESDTIGGFNSMLAKQKKVDGECF
                     VTTVLFDNDYELIHDRIDIKAIAPITEKEYYVGGSTALIDAMGKTINKIDNVQKNTAE
                     EYRAEKVMFVIITDGQENSSKEYTADKVRALVERQKEKNGWEFVFLGANIDAVETAER
                     YGISRNRAVDYLSDKKGTALNYRVMADAVTSFRKSGEVDDSCFEEIRKDVKKRGKKK"
     gene            30229..30921
                     /locus_tag="PLF14_03080"
     CDS             30229..30921
                     /locus_tag="PLF14_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70312.1"
                     /translation="MKDFLSKVLPSSLMVGIMYAILTAGGYLREIRIGDYDTPKVIRD
                     GNLSMFKSVIITNSILLGILIILISITLIAILTLMFKNKEIDYRVKIGIILAVFIIFM
                     ISYFCRNTPYTENDVYDTIESKAEDYITDLTNKEYDKMVKNYVLNRNMNKFVSKNIYS
                     QAMERTIREYGQIGKIEDPVKVYINEEYTFLSYPVIHGNGQVVYYNIAFNSKNEISGL
                     FTTLILPDRNNN"
     gene            31263..31943
                     /locus_tag="PLF14_03085"
     CDS             31263..31943
                     /locus_tag="PLF14_03085"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007157173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT70313.1"
                     /translation="MNRKELIKNAYRLTGSNNFYDGMITCSTLLGRAVSRLVWDMNKE
                     DCTDYQMQALLGIPENFSGRLLEVPVGTGVITMPFYRTLSDADITCLDYSPDMMSQAK
                     VRAIGLGLENITFLQGDVGALPFRDNCFDAILSLNGFHAFPDKEAAYNETFRVLKSGG
                     VFCGCFYVSGCHKRTDWIIRHIYQPMKFFSPPYETMDSLRKRLGSMYVDFKISNVKGI
                     ACFNCRKE"
     gene            32017..32733
                     /locus_tag="PLF14_03090"
     CDS             32017..32733
                     /locus_tag="PLF14_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70314.1"
                     /translation="MQENINWSKISSLMKIGLLGAFIILIGDFLIGWGVKDLSLPGIE
                     GQISQYLTISDSRFFWSSILGVIGVPVAAVGHFGIYRLLKPYSKKYAKLYAIGGLGFL
                     VFGGAGVHLSSVAAGFFYKYMATASPDMALASSIKYFCYFPLPLCIALMLFWIIMGYA
                     HIRAVAGGFSPYPRWCWVFAMPAGTLLITLITFFGNHAIVNAITVGAFSIGNIWALGG
                     HLLMLNKAKENYDSKGNRNF"
     gene            32757..33599
                     /locus_tag="PLF14_03095"
     CDS             32757..33599
                     /locus_tag="PLF14_03095"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT70315.1"
                     /translation="MEKIKIEKNSVQETLIIPLYGRKMCSEKFPELYTDIYAERLCNR
                     IDYDFSELEKKNKSFLYEFGSLEAAMRQLDMIWEIKDYLKKYPKATVVNLGCGLDDTG
                     KACDNGLCHIVNVDFPDVISIRKQLIIDGEREKNIACDLKDYSWMEQVDGSEGVIFFA
                     AGVFHYFKTEEVKNLVLELSKRFPEGCLVFDSVGKLGLKIILNKVLKNMDINDVDGYF
                     YINNPKKELNWSENIKVTSKGYMLGYYDMKSPGIRFSHRFFAWLGDKMMKMYVHKMEF
                     AGKE"
     gene            complement(33650..33892)
                     /locus_tag="PLF14_03100"
     CDS             complement(33650..33892)
                     /locus_tag="PLF14_03100"
                     /inference="COORDINATES: protein motif:HMM:NF018016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HOT70316.1"
                     /translation="MKTVDRIRAEIVKLYRTDPNIHIDVSMNRPRVRISNQEARIKSV
                     YPHIFNIETQGKCYSVQYTEILTKNVRIVELEEHNV"
     gene            complement(33894..34154)
                     /locus_tag="PLF14_03105"
     CDS             complement(33894..34154)
                     /locus_tag="PLF14_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70317.1"
                     /translation="MKRVKSACIFQTLVFVQKPEYGFSKEHALKINREEFEHYIAVLE
                     RSKTRYLIDDTVEQEDGSIVVRIRKQYNDKTDVSEYFKKNGG"
     gene            34409..34789
                     /locus_tag="PLF14_03110"
     CDS             34409..34789
                     /locus_tag="PLF14_03110"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOT70318.1"
                     /translation="MKTTKVRICNTEYVIKGDESEEYIEKIALFVDKKMNSIIRENPK
                     LSLSTAAVLTAVNVSYDYFKERDQANKQKDLMDEFEKKSKDQVSRVRLLEAKIEKLTQ
                     ANSELKETLTKTEEQLKKAQNEFR"
     gene            34786..35268
                     /gene="dut"
                     /locus_tag="PLF14_03115"
     CDS             34786..35268
                     /gene="dut"
                     /locus_tag="PLF14_03115"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOT70319.1"
                     /translation="MIKVYIEKTRKDAKIPVYANSFDAGADVCACEDAVLDINSTRVI
                     PTGLKVAIPEGYEIQIRPRSGLSLNTRLRVANSPGTIDAGYRDEIGIIMHNTSVVNEG
                     DFYVTEKHNRQGVYHIKKGDRIAQIVLQQVPKIEFVETDCVADIGKDRGGGFGSTGVR
                     "
     gene            35246..35884
                     /locus_tag="PLF14_03120"
     CDS             35246..35884
                     /locus_tag="PLF14_03120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOT70320.1"
                     /translation="MARQGSDKAVYTIKQDAVSEITEKKSRFISYAIKTETEKDALAE
                     LEKIKSKYPDATHHVYAYVVNDQGVVYHRYSDDREPSGTAGLPTLDVLKKGNIENVLL
                     VTVRYFGGTLLGTGGLTRTYGLSAKKAVKQAGLVKRVDCAVFTLTADYSSYEKIKRLL
                     DECVVMDTEFRELVTVRFAVEDLKAEERIGRMNELLNNRCVIERKNNIVLEI"
     gene            35886..36617
                     /locus_tag="PLF14_03125"
     CDS             35886..36617
                     /locus_tag="PLF14_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOT70321.1"
                     /translation="MAGHSKWANIKHKKEKSDAARGKIFTKIGREIMVAVKAGGPDPA
                     SNSRLRDVIAKAKANNVPNDTINKSIKKAAGEGDSENYEQITYEGYGPNGVAVIVEAM
                     TDNRNRTASDVRHYFDKFGGNLGTTGCVSYLFDRKGVLIIEEKESIDTEQLMTDALEG
                     GAEDFEHEDEMYEIICDPAEFSSLREALEQKGYEFLEAEVQMLPQTYVTVEDDKVESM
                     QKLIDNLEDLDDVQNIYHNWEQPEE"
     gene            36679..39036
                     /locus_tag="PLF14_03130"
     CDS             36679..39036
                     /locus_tag="PLF14_03130"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HOT70322.1"
                     /translation="MQKRINVSVRDIADFSLASGDIETYAAFGYREAALDGTQNHIRF
                     QKDMIKQYGEDCFAKEVYVEDVLENDVVYMVVTGRIDGVLKTDDSNIFVYEIKTTCKD
                     INEIKPADRPEHLGQAHCYAYMYCKKKNIDATGIRLVYINRENQDSITFEYRLTFNQL
                     ESIYSELTNRYLDWVTGITAWKTIRNQSIETLEFPFASYRKGQKKLMSEVYNCIDDAV
                     KLFATAPTGIGKTMGVLFPAIKALGAGLVDKLMYLTAKTVTAKVAVDAYKKLEKNGLN
                     LRTVCITAKDKVCPMEKKDCDPEKCSRANHYYIRARACLKEILHEQFFDRDIIARYAD
                     EYNICPFELSLDVSGYCDLIICDYNYLFDPRVRLQRYFDYENEQSFCFMVDEAHNLVE
                     RGREMFSCSIGIKQLEDFKDSIKKEWKALRKSVNALINEMKELQAEHFSTMNQGFISQ
                     AELPLVLTDRANRCVNDIMMYDLSKIPDDKRESFLEGMFNILFFSRVAQMYDRRFTTL
                     FTSDGKGLDVKLMCLDPSYLLRQSMDTGRSAILFSATLEPKRYYMDLLGGDGNMDSFL
                     TLDSPFPRENLKVKLVTDISTKYKDRDLSYEKIAQLLKKQFSQKTGNYMVFFPSYAYM
                     RRVVEIFTDLCPDMDILEQKPYMDDAERSLFLERFEKFGENTLVAFAVMGGLFGEGID
                     LVGEMLSGVAIIGPGLPMLCPERELIKKYFDDIDMNGFDYSYTFPGMNRVLQAAGRVI
                     RSETDTGFVLLIDSRFGTAKYRSLFPKWWNCSNMYNSFEEDGFLC"
     gene            39030..39491
                     /locus_tag="PLF14_03135"
     CDS             39030..39491
                     /locus_tag="PLF14_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HOT70323.1"
                     /translation="MLKQYLYILAISAVPVIEQRGAIPLGILLYKLDPVLVTLVSYAG
                     SLIPMFFVIYCFTPIFKWLSTKKILKWFYDFVDNKVRKGKEKVERYEVIGLTIFVAIP
                     LPMTGVWTGSAVAAFMQMNKKKAFISVSIGAAISAIVITVICYVAPKLLGY"
     gene            39548..40393
                     /locus_tag="PLF14_03140"
     CDS             39548..40393
                     /locus_tag="PLF14_03140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOT70324.1"
                     /translation="MKIVEYKESDISDFFPLRKDISCKSDYGRFLVIGGSPGMTGALR
                     LCVDACYRSGAGLVYYTAPKKDLLIYDLLTVEGITVPKEDIISLTGDKDCICIGCGLY
                     NDEENLILLNKVLENSKCNIVADAAALRMLAEHKELLKRHGNRMTVLPHPGEMSHLTG
                     LSVEDIQKERVGTAYTFYERYGCITVLKGNRTVVAGNDSVYINNTGNPGMAVAGSGDV
                     LAGMVTAYSVMSNDMFKNACMAVYVHGLAGDLAVKDLGVISMISSDIIRYIPQAHKEV
                     ACGKA"
     gene            40380..41525
                     /gene="alr"
                     /locus_tag="PLF14_03145"
     CDS             40380..41525
                     /gene="alr"
                     /locus_tag="PLF14_03145"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOT70325.1"
                     /translation="MEKPDRVWVEIDLDNIAHNIASIRKKIGDVKVAAVVKADGYGHG
                     AVEIAQTLCENGVYMLVVATLDEGISLRRNGITAPILVLGYICPEKIEQAIDNNLAAT
                     ITNSDMAVKMSSAAKEAGKTLYAHIKIDTGMHRVGFAYDDKQSILSALALCNIHYSGI
                     FSHFSTADEEDKTYTNMQYERFSGVLDSVKKAGYDIALKHICNSGAIILHQDKYMDMV
                     RPGIIIYGLYPSQYCKNHDMSDLRCAMSFKSRIIQIKKVKKGQPISYGNSYITERDSI
                     IATIACGYADGYSRVLSQKTHVFINNRRAPIVGNICMDMCMADITGLKGISVNDEVEL
                     FGPNIPVEHLATICSTINYEYICKIGARVPRVYIKEGNIVKINNYLV"
     gene            41601..42194
                     /locus_tag="PLF14_03150"
     CDS             41601..42194
                     /locus_tag="PLF14_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70326.1"
                     /translation="MRQDKKTAIAIITVAFIMSLMFCTTAQTADLVIASVEYVDAKIA
                     EVRALIQSSGTPSGGTTPVTPVDNAKITELETALGNALKEMDALRSRMDFLDVTVRDM
                     SDNYGMQVLHVYKDDVIYALGTSELILRSGSAIVIGNANNEGLSDATTGENLNPGVSL
                     GKDHFVVIPRGDGRGIKITSGESYIMYRGSFSYVPAN"
     gene            42178..43281
                     /locus_tag="PLF14_03155"
     CDS             42178..43281
                     /locus_tag="PLF14_03155"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HOT70327.1"
                     /translation="MYPQTDIKSYMPVKILFGNNCVFNNKDVFKQTGKFPFIVTGKSG
                     AVKSGAFDDVISVLNGGPYILYDRVEENPKVSDMEDAAIISKKFRCDYIIAIGGGSAL
                     DAAKAIAVLAVNNMSGMDLFSKKADAALPVIAIPTTSGTGSEVTPYSILTVDEWQTKK
                     SFAGDCIYPKIALLDPKYTFSLPIDYTISTAFDAVSHLFESYFSLKSNEMSKMYSQEG
                     IRAFAKAVDAIKNRKFDEQSRSALMYASMLGGIAISHTGTTAMHAMGYSMTYFHGLPH
                     GFANLYFAEPYFRHMMKQEEQKTKTVTDLMGMTDLQSMINFFSDNIPRPKLNKEQCER
                     YATLAMMQSSIKATPGNLTKEDIVNMFKEAGRT"
     gene            43278..43862
                     /locus_tag="PLF14_03160"
     CDS             43278..43862
                     /locus_tag="PLF14_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70328.1"
                     /translation="MIVYYHIPEDKAKDAISFGMKLSEYGNFLINIGNGPKNVITAYL
                     CPADDIKRYNRSGYTCLCIDFPIEKTYIMEGVYLETENTQALKASLTKAAEYRLGSYR
                     KPLALITTTVFAERIKVLGKYMDVPMIVDDSEELYLKNALLELEEGDSGYYEKALYGY
                     LTVTKEAHMLYEKNGYAEYILNKRKYIVRKPDGK"
     gene            43862..44617
                     /locus_tag="PLF14_03165"
     CDS             43862..44617
                     /locus_tag="PLF14_03165"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOT70329.1"
                     /translation="MCDVEIIKLPYINSTNTYAKDNAQSFSKKITVIQAERQLQGRGR
                     LGRDFLSDNSDGAWFSVAIKPEKNTISPQKVLLMPILAAGETAAAIEDIYGIKAGIKW
                     PNDILINGKKVCGILCESRVVDNNVLLIVIGIGINVNQKAMHPDIEDIATSLFMETAT
                     QVSVDLLIQNICGNIVCSYDRMRRGNTDGIIDKWNEYSIMNNAVITYTKGQSLHSAVS
                     KGIDRQGRLIAEQDGLTIYLDSSEISIKSTRSK"
     gene            44617..44934
                     /locus_tag="PLF14_03170"
     CDS             44617..44934
                     /locus_tag="PLF14_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70330.1"
                     /translation="MNDNRNTASANDGNRKRSNNNYYRKKKNYSGERKQNNLPAQVTA
                     GEEKDNRKSSQNKGYNNYKRYNRDRRRNRSGEETVDDIKKDIMRIEKEIRLEIEEIKM
                     IRV"
     gene            44938..45744
                     /locus_tag="PLF14_03175"
     CDS             44938..45744
                     /locus_tag="PLF14_03175"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009644257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOT70331.1"
                     /translation="MLLAIDVGNTNIVMGVHNGEKYTNVWRLTTNKERTPEEFGVFYI
                     NMFNAAGLKLSDVDAIILSSVVPGIMYTLTNSLEKYFNRTPLLVEQGIKTGLNIKIDN
                     PRELGADIIVNLVSAYETYKGPAIVIDFGTATTFSCVTDNFECPGVVICSGVSLTAEA
                     LYKRAAKLPNVDIIKPAKVIGKNTITSMQSGIVNGYLGLIAGIVKDMKKELAEDYNQN
                     YEDIKVIATGGLLSIIAKNSDQIDYIDSMLTLNGLKLVYDKNKSKTEGKA"
     gene            45752..46387
                     /gene="tmk"
                     /locus_tag="PLF14_03180"
     CDS             45752..46387
                     /gene="tmk"
                     /locus_tag="PLF14_03180"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOT70332.1"
                     /translation="MAKGMIISFEGTDGSGKTTQIKALSRYLEEKGYMPAILREPGGT
                     RIGEEIRSILLNSDNTEMASLTEMLLYASSRAQMMEEKVEPLLKEGKIVILDRFFDSS
                     LAYQAYGRDLNFDAVLSINLAAVKNIVPKITFFIDITPEQSLKRRQNATATDRLENED
                     MSFHYKVYEGYKKLLEKFPERIIRIDGTKEAREITNEITSYVDNVLKEDIR"
     gene            46384..46713
                     /locus_tag="PLF14_03185"
     CDS             46384..46713
                     /locus_tag="PLF14_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HOT70333.1"
                     /translation="MKLIFAIVNDEDELDVMRGLSQEGYGVTKLCSTGGFLKSGNTTL
                     LIGVDDEKLDHAIEIIKARSKSRKKIVNSSMIGASQGAVLTGYPIEITMGGATIFVTD
                     VVKYVKS"
     gene            46715..47668
                     /locus_tag="PLF14_03190"
     CDS             46715..47668
                     /locus_tag="PLF14_03190"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta' C-terminal
                     domain-containing protein"
                     /protein_id="HOT70334.1"
                     /translation="MADFDDIKGRKPLKNQISKLIKKGRICHGYIFEGPVGSGKKLIA
                     DAFSRLLFCKNPDKLKVCGHCEGCLGHKEQYILKSDKSISVEDIRTLIKDTQLLPLGS
                     GNKIYIIHDADTMTDQAQNAFLKALEEPPSNVYFLLTVTNSEQLIDTVRSRCVIIYTG
                     VNTESEVKDYLQNKYPDTDIQQINFAACFSEGCIGRADTLITDETFRQTRSAAVRYLS
                     EINTDLINKDKSIDITVFLEVLQSLFRDALIFKETEQTDSLINADFLDIIKRTAANNS
                     SESLFKACNYVAKAAKYIKDNVSVSIAIGTMNVELMEELNI"
     gene            47665..48552
                     /locus_tag="PLF14_03195"
     CDS             47665..48552
                     /locus_tag="PLF14_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HOT70335.1"
                     /translation="MITVVGVKFRHSNKIYSFAPGDWELNINDKVIVETIKGTEMGTV
                     ASPITQVEQSNVVAPLKEIIRKATQEDLNKAEESKSKESEAFAICEKKIAGHKIAMKL
                     VGVEATFDMSKIIFYFTADGRVDFRELVKDLASTFRTRIELRQIGVRDEAKLLGGIGV
                     CGRVFCCTSYLNDFQTVSIKMAKDQAISLNPLKISGVCGRLMCCLKYEHEVYTDLIKK
                     TPGIGAIVQTADGKGEVIDVNMLKGELKVKLDKGEEIDIRQYNAQDVTVLKKSSKYKV
                     LPDEEAAQDINELTKLEDN"
     gene            48643..48813
                     /locus_tag="PLF14_03200"
     CDS             48643..48813
                     /locus_tag="PLF14_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOT70336.1"
                     /translation="MPYRITDECISCGACEAECPVEAITQGDDIYVIDPELCISCGAC
                     ADVCPVDAPVEE"
     gene            48874..49512
                     /locus_tag="PLF14_03205"
     CDS             48874..49512
                     /locus_tag="PLF14_03205"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOT70337.1"
                     /translation="MTNHYYTDKQLTHEDMFEYKARINEVEFRFYSDSGVFSKTGIDY
                     GSKLLIDSVIADIGEQEKGLSNKERPSLADLGCGIGVIGIVLNRLLKINNTYLYEINE
                     RAAELAKINAGQNRSKRTYVKKLDIVKEDIPEKADICVTNPPIRAGKATVFAFYEKAY
                     EMLNENGIFYCVIQKKQGLDSTKKKLAELFSNLETINRSAGYHILKCIKDKN"
     gene            49543..50373
                     /locus_tag="PLF14_03210"
     CDS             49543..50373
                     /locus_tag="PLF14_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70338.1"
                     /translation="MKVYALVGPSGTGKSYKSTLLASDMGITHIIDDGLLISENRVIA
                     GKSAKKEATKIASIKRAIFDDNDHRKEVIKAIRAGKVDSLLILGTSDKMVNEIAQRLE
                     LPSIQKIIRITDISDEEEIAAATRSRQEKGKHVIPVPTFEIKKDFAGYLLDPLYIFRR
                     RDKTRYEPDGTKTIVRPTFSYLGGYTISNNTLFQIASYGCTEINGYEGMTRFRVINRP
                     EGISLEVNITVNVDYDIPETLRQVQQKIYDVLDFTTALNMIRIDVTAKNIMINGEVYP
                     "
     gene            50518..50811
                     /locus_tag="PLF14_03215"
     CDS             50518..50811
                     /locus_tag="PLF14_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70339.1"
                     /translation="MSILKQVLIEELERNNHFIDSAEKEISQLPIGYISKKNINNKTY
                     FYYQWREGEKIKSEYIKKKDVQNLDKKIKRRKELEEAVLKAKNENKRIERALK"
     gene            51046..52359
                     /locus_tag="PLF14_03220"
     CDS             51046..52359
                     /locus_tag="PLF14_03220"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOT70340.1"
                     /translation="MDSDSIYRSIALIGLIVVGGLLAGMETAYSSCNKIRMKYNAENG
                     DVKSQRVLKILEQSDKYISTILILLNVIHITAASIATVIAVRYLGEIGSLVSTIVLTI
                     LIFTFSEVIPKSIAKDNADAYSRTFAPLLRFLIVILTPIAYVLSAVGRLVRKLMGNAP
                     KEPTMTEEELQSMIETIEEEGVIEADKSELIQSAIDFLDTTAADILTPRVDIVAVDID
                     WSKEKIDSIVIKEKFSRIPVYENTIDNIIGVLHSRKYLKGILNNTAVDVASVMDEPIF
                     VHQSLLVNTLFEMMSARRVHMVIVTDDYGGTAGLITMEDIMEELVGDIYDEDDIVEED
                     FVHLDDGVLEVVGELRMDELLDELDIPCNDDDIEHKTVSAWTMEHLQNIPEVGDCFED
                     MGLSVSIQELDGMRIEKLLVKVIEKKHDENPEEENNRKKQEEVNR"
     gene            52359..53624
                     /locus_tag="PLF14_03225"
     CDS             52359..53624
                     /locus_tag="PLF14_03225"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOT70341.1"
                     /translation="MDWLIYLGIIILIMLSFFFSGTEMAFSSLNIGRIRLEAEGGDKK
                     KKKTYELAKNFSSVLYTVLVGNNLVNIAISSLTTLIALRIIEQTNTTLPVQTITVIVT
                     TIVILIFGEITPKMVANDNPNRFAVNVTWLVNVFIFIFKPIVFTVTKMTEKLSVIWKP
                     KKEEPTVTEDELLSIVETIEEEGGFTEKESDLIRSAIEFSDDRAFEILTPRVDVFAID
                     INDEPEDIIRAVVDEEYSRVPVFEGTIDNIVGILSTKRLLKAAVKKGAQIDIRSMLRE
                     PLYIYKSMDISSVMEEFKAKRAHMAVVVDEFGGTMGIVTLEDVLEQLVGDIWDETDDM
                     SEDTVETAEGTYEVAGDMLLDDFLEMIDFDEELESESTTVGGFVTERLGRLPVENDRV
                     EFDGHVITVLEMANLRVERIKVEMRKDAD"
     gene            53614..54252
                     /locus_tag="PLF14_03230"
     CDS             53614..54252
                     /locus_tag="PLF14_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT70342.1"
                     /translation="MLTDNIKWLGHAAFRVDGSKTIYFDPFRIDEGKKADIILVSHKH
                     HDHCSKEDILKILKPDTLIVTEADSAEILQLDNMIIVKPYDSISVEGIEIQALPAYNV
                     TKPHHPKENGWLGFLINIDGKTIYHAGDTDCIEEMRDITTDVALIPIYPDNKYVMTPE
                     EAAQATYYLNAKVAVPMHYGVLGGSKGDALTVAKKGNPAIQIVIMESRKNSF"
     gene            54376..56199
                     /locus_tag="PLF14_03235"
     CDS             54376..56199
                     /locus_tag="PLF14_03235"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HOT70343.1"
                     /translation="MEKKRKRRQAFKEKAAIILLTVLYLVISLTNLGNTKAPVNGWTP
                     QKAGSVVYVDFEKEETIERITYLCGLGDTWYNVTALDLYYKDASGYYVYFTTLERRAS
                     TFLKWLYTDLEQPVTTDCVKVVSGIYQDKEEDYIRGTRGEIMEVCFFASDKDTPIPIS
                     SVRPTDPHKDEKSVNLFDEQDLYVYEPSYMNSTYFDEIYFPRTAYEFLIKNQTIYENT
                     HPHLGKIIIMWSYKLFGVNPFGYRAMGAIAGALMIPVMYALGKKLFRNHYFAMIGATL
                     LAFECMHFVQTRLGTVDSYLVLFIMLTFYFMACYVDMDLRKTSYFKTLVPLFFSGIFF
                     GCAISVKWIGFYAGAGIAFLFFVKIFSQWKSFGYGRIVYGGKALAARNSLLTCFFCLI
                     FFIAIPALIYSLSYTPIFQIQSAAIDNEKFTIMEMADNIIASQKHMFEYHKGVTQDHP
                     FKSSWYTWPIMYKPVYYYSAPVKAEGQWGTIVAIGNPLIWWTGLAALIGTAIVSLRRQ
                     DRRGLFIFAGYLSILLPWAVAPRSITFLYHYFGCVPFMILAIVYMASYFMEDNPKVKK
                     YINAYVILAILVFAAFYPAMTGIKVSVDYIELLRWLPSWWF"
     gene            56200..57411
                     /locus_tag="PLF14_03240"
     CDS             56200..57411
                     /locus_tag="PLF14_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOT70344.1"
                     /translation="MRELYDVSASEHNPKWENMICRKEHIGRKENDIRSEFYRDYTRI
                     INSRAYRRLKQKTQVFFATRNDHVCTRMEHVNNVLSISYGAAKYLGLNEELVMAIAVG
                     HDLGHSPFGHSGERIIDRITKEYLKTDFWHEKNSLRVIDYSETIQNAWGQEENLNLTY
                     AVRDGIVTHCGEIERKQLVPRTEYIELEDIKNASSVSPYTYEGCLVKAADIIAYLGRD
                     IEDALRLKILNSQDISRLDEILKQYKKADEAMNNSTIINSLVTDLCFTSKKEHGICFS
                     DEGFMILKKIKAFNYEYIYKNKRLRKYEEYAELIIQSLFDVLMSFYKEGDTLNYIKTE
                     YSTIYPELASSFSDRLKKYSIMENRPDNLKNKMLYNLSSKEDYIIAVIDYISSMTDMY
                     AKKLFDEITSF"
     gene            57471..57887
                     /locus_tag="PLF14_03245"
     CDS             57471..57887
                     /locus_tag="PLF14_03245"
                     /inference="COORDINATES: protein motif:HMM:NF022338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOT70345.1"
                     /translation="MEIKEQVGKRKTGIILSAVIVAAIAALLIFSKVSITVGEQAITV
                     KAGLERSVIAYEDIVKVEKVSSITVGKRIMGADLIKIYSGTFKNEQYGRYRLYIYKDV
                     SNYIVVEHKEGFAVFNSDTTERTDSLYEDILEKLKR"
     gene            58011..58811
                     /locus_tag="PLF14_03250"
     CDS             58011..58811
                     /locus_tag="PLF14_03250"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT70346.1"
                     /translation="MNGLYKIADSHTHIYPDKIAMKASKSIGDFYGIPMFYNGTVKEL
                     LENGSRINIKKYLVCSVSTTSQQVISINDFIISQCLEHEEFVGLAAMHQDFNNVEKEL
                     ERVAEQGLKGIKMHHDFQKVYSDDERFFRTYKKAEEAGMVCLLHAGDDRYDYTRPKRI
                     ISVMEKFPNLKCIAAHFGGYKCWEEAYKTYKGTDNLYFDTSSSLYSLKPDEARDIIRE
                     FGSDRFLFGTDYPMWDAKTELERFLSLNLTERENSSILYDNFINLFNL"
     gene            complement(58801..59520)
                     /locus_tag="PLF14_03255"
     CDS             complement(58801..59520)
                     /locus_tag="PLF14_03255"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOT70347.1"
                     /translation="MDIRLMTYNICSGMSYGENRKRDLAQSAEVIKEYAPDILALNEV
                     HYNIGFSGFVKQAEEINKLLNYEYMYYGKAGAIKEGFQGNALFSRYPVKTVHTVLVDQ
                     PAVLDEDVYYEQRSFIDSVIDIGKDLHVIVTHFGLAEGERKNALDLLIPIFRQHRKSL
                     VFMGDLNLEPHEEQLKSISGILKDTAAISNKKYFTFPSDIPDRKIDYIFVSYDIKVND
                     VVVPLTTASDHLPYIADVFIG"
     gene            59693..60523
                     /locus_tag="PLF14_03260"
     CDS             59693..60523
                     /locus_tag="PLF14_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009254444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOT70348.1"
                     /translation="MSLPIALQLYSVRQDLDKDFEQVIKKVKEYGYDGVELASLHGKE
                     PEYVKKLLSDTGLTPISAHVPLDSMLKDPDGVFGTYAFIGCKYVAVPYLPEERRPGKP
                     LFDKTLEQMEQLGKKAKEAGLQLLYHNHDFEFVKIGDRLGLDVIYETIPADLLQTQID
                     TCWVKVAGQDPAEYIRKYKGRAPVVHLKDFYKQETDKDIKMYELIGIKGKDHKPSDKK
                     AFGFMPLGFGQQDIPEILEASKEAGASWVVVEQDEPQEGRTALECARLSIKYLKNLIW
                     "
     gene            60538..>61019
                     /locus_tag="PLF14_03265"
     CDS             60538..>61019
                     /locus_tag="PLF14_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT70349.1"
                     /translation="MSEDILNKFKKIHEEKKSTDTGKVLKIGIIGTGWIAEPHVNAYL
                     KCPDAKIVACADLIDGKAAAFCKKMGLEDVRLYRSHKELLDNEELDAVSVCTYNCTHA
                     ECAIYALEKGVHVLLEKPMCVTLDEAVAIVRAEKASGKVLSIGFQPRFDENMKMIKKI
                     V"
BASE COUNT        19588 a        11037 c        13522 g        16872 t
ORIGIN      
        1 tataatattt ctataaccgg acttaatctt ttatgtaacg gacacaactc caaagagggt
       61 aatctcatag tcggccttag ggggcaggta tgtttctttt atgttaagga tttggttata
      121 cgtgaccttc tctgtttaga ccttatggaa cacagtttct gcattcatat atgtacattt
      181 gagaatcttt taatagagaa tgtgcacata gaagggctta aagacggtat acatatagat
      241 aagggcagca agtttacaat aaggcacggg gtatttaaaa cattcgacga tcccatagca
      301 ttaaatgccc atgattacac aagctgtgct ccggaacttg gctggattga agacggcata
      361 attgaagact gttatgattt ggatcaggaa aagactacag gctatttctg cagactgctt
      421 gcagggtcct ggcttgactg gtttaaagga atgaaagttc agcaatcgga taccgtcgta
      481 tacaacaata agatatacag ggtaaacatg cagccggacg gcaaaatata tacatcaaac
      541 accgctccaa atcacagcga aggaacaaag gaatatgacg gtataaggtg gacaatgtta
      601 caggaccgag aaattgtata taactgcgga tgcaggaata tacattttag gaatatcttc
      661 ttaaaaaaga aaagacatat ggcatttgct ctgacctttg ataggaatga atattccaga
      721 ggttattatc ccaactcaat acctcccgtg caggaagata taacattcga gcgtatatat
      781 gtacagaatg aaattatcga cttattaggt attagaacgc cggtaaatac aataaaactt
      841 accgattcgg taataggtaa taacaggatt tatctcagat atcttgaaga agagggaata
      901 acatatatcc ccgcacatgt acttatatcc aacacaactt ttaaaggtga cggcaaacag
      961 agccttgtat atgcggaaaa aggaagaaaa gcgacattaa agattaaagg ctccataaaa
     1021 gagaatgctt cctatgaacc tttgatagaa ggggatatcg aaatactgaa aaaggatata
     1081 tagtacggac atattccgcc gacttgcaca tcaaaacagc gccgacttgc acattcgtgt
     1141 tgctgtcagg aactgatgat gtgtatatgt aatacctatt cggtgtttga agataaatgc
     1201 tcggacataa ccattttaca attttcaaca tctttatggt ctcagcgcgg taatcggtac
     1261 tacataaact ccatcttccc tttgataagc aaaattgctt aatccgcata tgaccaccaa
     1321 aatatccgga attcttgctc cttcagcttt taaatcatct ctgattttga ttagtgattg
     1381 tgcagctttt tcaatagcat ctgcaccgag ctttatttca aaagccccga aacgaccatc
     1441 tctaagtttg acaactgcat caatttctct ttccttgtag tcttgataat ggtacagttg
     1501 agcttccaag gattctgcgt atattcttaa atctcttaca catagcgctt caaacataaa
     1561 accgaatgtg tgcagatcgt ttaataaatc ctcataactt gcttcaagta tcgctatagc
     1621 taaagaggga tcaacaaaat gtctcttttc cgattgctta atgcgtgttg atgaccttat
     1681 attacttgaa aaggcctttt ggttttctat taagaacagt ctttcaaatg tacttaaata
     1741 tgttgagatt gtatcattgt caagagtatc gttatcaaaa tcggaaatat cctttttaag
     1801 agtagcaata gaagctgttg tcgattcatt tcttgcaaga gattgtaaca ggagttgcat
     1861 tttggccgag cttctgttta ttccttccag tcgataaata tcatgattta tgattgactt
     1921 aatatactct ttcggaagta gcctgaattt ttcataatcc aatcctaaac tttccggcca
     1981 accgcctcgg attgttaatt ttataagggt ttccaaatct atattgccgg tggctactgc
     2041 cttaaaagta ccatcaaaca aagaggtcaa tgatacttgt cccgatgaat cattcgattc
     2101 aaataatgac atcggtctca ttcttatcgt agatattctg ccggcaccgc tatgcaaaat
     2161 tcctttttgc ttcggtgttg acgagcctgt taaaataaac tgtccttttt tacctcttct
     2221 gtctacttca tgtctgactg catcccaaat agaaggcact tcctgccatt catctattaa
     2281 tctgggtact tctccttcta aaacagttgt tggttcgagc tttgccaatt ccctgttttg
     2341 aaaatttctc gacggatctc ctaagtatat ggcgcttttg gaatggtatc cggaagtcca
     2401 tgttttgcca caccatttag ctccttcaat acaaacggcg ccaaaggctt caagctccag
     2461 ttttacctgt ttatcaacta atctcggttt atatagctct ttatccatag ttacctccga
     2521 cacgagtata acatgaatcg gtcatatttt caatttctat tcggttaaat ttgcaatttc
     2581 tattcggtca tattttcaat ttacattcgg tcattttttg gaatattaaa cttgattaat
     2641 gtttttcgac ggtttcattt aatgtttttc ctgcatatcg acgatttcat ttagcgtttt
     2701 ccctgcatat tgacgatttc atttaatgtt tttcgacaat atcaatcaat gtttttcgaa
     2761 gtattgatta aaaacaatgt atatttgatt attatgaaag tacggctact gtttcacaaa
     2821 tgttatcttg gcgtctttgg gtaaagccag attacattca acttccgccg cttttataat
     2881 ccctgctata gcgggacatc cgcagtggcc gggcaggtgc tcttttgcat agtcatataa
     2941 aggtccgctt cccggtttac cgagacacag ttcatatgcg tcaaatgtgt cgcctaagtc
     3001 cttaaacata tcattaatgc ttttgcatcc cgatttaact ttcagtgtaa cttccattcc
     3061 gtcatccgag gatgcctcta ccgatgtgac aaggttgcat atacccggat ctatcattac
     3121 ttttgtcata ttacagaata tcctttcaaa tgttttacat gaatgctatt ttacaccaat
     3181 atacagtcgt atggaacatg taacagtgat attatcactt ccctaatatg tgtaagactg
     3241 taaatcatta catgaacatg atagaattca cgtataaaag cgggggtggt atcatgggca
     3301 ggtatacgca gaaaatatct ttaaggctta ttcctttacc gaaggagttg tcatgcaatg
     3361 acattaaaaa agtggcattc aacgatataa gaatagatgt aatatatccc gaagatgctt
     3421 tgctgcgttc ggctaaaaga aagctggtat cgtttttcgg caattccctt tacggctctt
     3481 tcaggataag aatagtggat cctatgccgg aaaccttgaa tgtgcttagt cattttccga
     3541 attccgatca agcatatcgc atatggtccg acggatcaat aaacatatct gcactgactt
     3601 ccaaaggaat aatgaacgga gtacgcacat tattacaagc cgtatcaaag gaagacggca
     3661 tgttcaatat gccgcttctg ttcatatatg attatccgga cataccgtta agaggacaac
     3721 ggagcgaaac agccgtgcgg gatatcgcat atatggcttc tttaaaactc aacagtgcgg
     3781 attatatgtc aattaattca attgatgagg aaaaaagatt gaaggaaaac tgcataaatg
     3841 aaggtatagt gctcaactcc aatatatctt attccggatg tattacagat gaaaatcttt
     3901 gtgttttatc ggaaaacgca gataccgtta tttttgattg gatgacttac ggcggaaaat
     3961 gtatattccc ccggaccgct gtagatttca taaaagaaaa ctgtgaaaaa tttgttgaga
     4021 ataaaacacg caaggttgta tgttacacac cggtatccga catatatcaa aagttcaata
     4081 taacggctat ggcggaatgg ttatggaacg caaaaagcag ggatgcggaa agcttcatga
     4141 gggcatatgc gaccgtcaat cagatggatt ccgacaaatt cgtaaaatgg tgtacatata
     4201 acatgactcc ggcacggacg cttgcggaaa acggatttat tgaaagactg atgtccaata
     4261 ggactgtgac ggtaaaaggg aaagaatact tggattactg cggatttgat gacttcatag
     4321 aaaatgcgat tatagatatt gataaatata tccgcatggc cgacagagcc gtaagagcgg
     4381 caaagcagac gggatatgaa gaaggtgaga ctgaaagcag atgcataacg gcggcattac
     4441 gttgtgcggg ggcacagcag aaattcttta cattgtatag aatgaaaaaa agaaatcccg
     4501 aagaggaaca acagatggta cttatgtatg agatgatgca taataactcc agaagcttgt
     4561 attatgctct ttcggattgg aaaaatgttg tcgataaaga gaatctgcat ttccatacgg
     4621 gaataacatg tacggccgtc gcatgtatga gaatggccga cattatgaag aagatattgg
     4681 aagaagagga tataaagcac atagagcatc attcgctttc ttatcatgaa atcggaggct
     4741 gggacgtgtc tgtgtttaac aaagaaggaa ttgcggagct gacgtatgat attgcaaagt
     4801 atgccgacaa agaaggccga tatcacattt gtttcgatta tacggacggt aagtccgata
     4861 tatcggtaaa aagcatatcg gttttataca ataatgttga gaaagcgcac attatttacg
     4921 acaatacttc ttctctgaaa cggttgaata acgatgagcc ttggtatgag gtatttattg
     4981 atattgagtg tacgggcaga gccgaagaaa aaatactgaa agtaacattg gaaaaggcta
     5041 cagacagtaa ggataaatgt aacggtattg tgggtataag gaagatatag ttatctgtat
     5101 tcggctattg tacgtacaag catttccata ttttcaaacg ggaagccgtt gtcacattcc
     5161 acatagaacc acgatccgcc ttcatacatg cggaatgttt cgtattctct ttttacaaga
     5221 tcccgtacct cggaaggtgt tccgaaagtc attacatttg ctctgtcggt atgaatagcc
     5281 accgcaagtc tcaatgaacg gcagtatttt gcaaatttct ccatgtcata tgcgggaagc
     5341 tgaggccata tggaatggac gcctaattct ttcaagtctt ccaaaatagg gagaacatac
     5401 ccgcaactgt ggaaatgaat gtttttaccg gctgctctta taggctcgaa aagcctgttc
     5461 catctgggtt tgaaaaatct tctccaaagt gcagggctca gaatcatacc tcgttcggtt
     5521 ccgtagtcat cgccgaaata gaagccgtct acatcaatag ctaaaagata ctgaattttc
     5581 ttttcgtaat attcaactat catatcggca agcatgttta tttccgccgt atcttcagcc
     5641 atgtcgcagt atatattttc gtcagggcat aatgccttta gtttttcaat catagtgcct
     5701 gcaaatctgg acagataata ttctttttta tgaagaacgg catcccgctt atcctgcaaa
     5761 aactgctcgc tgccgatttc agactgttca ggcattttgt aattctttaa atttgacata
     5821 tcctcaagag gatatatatg aggaattccc gctatgccgt atatcctgta ctcccagacc
     5881 gtaccccatt cgtctttttt gaattcatga taattgccgt tttcgtccaa ctgatctttt
     5941 gtgagtacgg gaatttcttt tttttgaaaa ggtccgaaat catcgggaac ggaagcataa
     6001 agttcgttaa gcttctctcc gtgctcgaaa tatcccacgg gagtgtagtt atattgcagc
     6061 ggagctttat ctacgggctg gaaatgtatg gctgcgtgaa ctctttcacg gctgttcata
     6121 atgtacctct acttgtgata tttatcaaag gcaaagtcgg catattaaac tatgtaacct
     6181 ttggttttca gttttccgtg tgcttcttta accttttcgg taattacttc aaccgctttg
     6241 tcctcgggta tattcatgca gacatattca tatcccttat cattaataac aggcgggatt
     6301 tcattatcca acggattggg tccgaaaaac tcctttaatc tttccagatt ctcctgtaca
     6361 catgtgaatt taataaacag gcatatatcc tgcagggttt tggaagatat ggaaaactca
     6421 tatttaaaag gtataccgct tacagtaata agattaatgg aacatcttac atcggacatt
     6481 ttagtgtcgc tctcggattt cagcggttgc catatttcaa aatctcctat cagattggta
     6541 aggtttgcgt ctacgtcatc tatgatagct ctgtttcgcg cccttatctt ctgatccgct
     6601 tccataccgg tgccggtctt tgcctcttcc attatggaag taaccgagga gaagaaaagt
     6661 atctctttca tatcattcaa ttcctcgtcg gataagagtt tgtctttgtc tatatccact
     6721 atggagacaa atgttttcat gaatgcacac attcttatga gttcgtcttc atctatcttc
     6781 ccgtctttac ctattaaacg caattcttcc ggaagacctt tgtagttgcg aaccacttcg
     6841 ggatcggcaa gtgtccgaag gccctttccc gtaagttcgt catttttagc acttgttaca
     6901 atgaaagcat acagatgctc ataggtcatc tgcatgcaga ttattgcaaa aagcagtctt
     6961 tgatgtaaat cttccctgac actggttttc ttgctgtccg atatcagatt cagcaactga
     7021 tatgtgttga aaagtctttt tatgcttctg ggattgaatc ctatggaatt tcttatgagt
     7081 tgcttatagt cctgtactat tctggaattc gctttcatgt tcattttttt gagcatgtca
     7141 tatatatact tatttatatc atactgcgcc accggcatct taaacggaag ctgtattatc
     7201 ttatcaaaga agctcttgcc tttgtcttca tccacaagat ttccgaactt ctgccgtaca
     7261 ccggccgtta ccacgctata gtcgacggct aaaatgaaca cacagttttc acagtctaaa
     7321 aacactttaa gtacttccag aagttccacc gctttgccgg gatttaatct gtctaaatca
     7381 tccacaaata tgacaacacg atctttttta ttgtctttaa gtttgttgtt tacgctttcc
     7441 tggaatgcgt ctttcaaatc gtttaccgct tttgctatat ccgagtcttg cttatcaaat
     7501 acattcacta tagcagcgcc gtcaagaccg accgaactga tggctgtttt ggctcctatc
     7561 ttcaatatct ttaaactggt ttccagaatc ttggaagttg cggtctccgc tttggattgc
     7621 agtttggtta tgaggttttc caaaaaagat aaacacaaat cattacccat attaaactgt
     7681 gaatattgcc aggtgttaaa ccagatggga tatacctcac cctcaatttc tcgttttatc
     7741 atattcatca tactggtttt tccgcttccc caatcaccct gaatggatat ggtcatcggt
     7801 gtatcacagc ttacaataaa ttggcataaa ccgttaatat atgtgcctat accaaaagaa
     7861 tcttccgtta gcttttctac cggtttatcc gtatatccca acctgctcat ttgctacccc
     7921 ctttgaaaca caatagattt ctaatacatt tatataatta ttacacatat tgattataca
     7981 caaaaatcat gaataatatc aattaatcat ggttttggat agtgtaaaaa accttgattc
     8041 ttctgaaaga gataatctac ccatcaaacc cggctttcgc taatatctat gtcggggtgt
     8101 agcatatttc tatttgtgcc attctttaat tttatttact aaaattacgt tgctctcttc
     8161 tcccgccttt gtgttggttt caaggcttcc gcccatttcc agcatcatac cgttaacata
     8221 aacataaggg ccaaggaaga agatatgtcc tgcgtcatca aagatataga tatccgtatt
     8281 ctccggcttg tgttctttta ttactcttgc agataattcg ctctgccaca ttttgtccat
     8341 tgcgcctgca aatatgagta tattcgcatc acagttttca acttttattc tggcagcctc
     8401 gctaccaggg tcattgtttg cagcactttc atagcccggt ctgtaatcta tcggtctgtt
     8461 aaatataaaa tctaaaatga gattagtcgc cgcttcagga ctcccctttg tcatatctat
     8521 ataggggagc tcttcacctt tataagtcca ggaagacatc atttcccggc tgtaattctt
     8581 ggacagaccc atataattcc aggcagtcgg tgcgctcagg ataacattgt cgatttcggg
     8641 atacaaagtt gccaaattga gtgcaagttc cgctcccttg gataagccga aaaccgttat
     8701 aatctcgtta tcttcgatgt ttttgtcaat atatgcgagg acttcttcaa aatagtccaa
     8761 gggaatccgg acgagttctt ctttttggtt ctccataccg aagaaaaaca aagcaagggt
     8821 ttcgaaaccc gctcttgcaa aggcctctgc agaatgatag gaaggggagc cttccgagcc
     8881 gccaaaggtt ataatgacac ctttatatgt tttattcagc ggcttcagtc tgaacccgtt
     8941 catatattcg cctgtaattc tgctgacttc tacaccctca atatttgtat tatattgcga
     9001 caaatccgta ggagacagag cctcaccgat gtcatgttta taacttttat atttgatacc
     9061 gttgtatgtt cttattccga agataagcat aaacgaagtg attataataa caagtatgat
     9121 tgcgattgtt ttcaatatgc caaaaaatat tttcattgcc ggctcctttc aaaaaagtgt
     9181 cattataggg agtgtataat aattttctgc aaatcggttt cctgtatcag attaatttta
     9241 gcaactgaaa aaactcctga aatccaagat tttcttagcc aggagccatt ataaaatacg
     9301 atatcggacg cgtcaaggtg tgtgaaacac agatagtgtt aaatgttatt taaatgttat
     9361 taaaatatta tttatgttac tcttttcttt caaaaatcat ctactgcttt atactttttt
     9421 aaactccgat taccgttact taaaaacgat taccgaacat tatggagttt ataatgctct
     9481 ccagcatttc ctgtcttccc gaagtattgg ttgtaatctc tccgttggat aatgcatacc
     9541 tggtcagttt ctccaaatcg gttttcccgt tgacaatatc ggcacctata ccgtgagtat
     9601 aactcttgta gcgctctttt acgaaatcgt caatcctgcc gtcgtctata atagcataag
     9661 ctattttaag acccaatgcg aatgtatcca taccggcgat atatgcaata accgtatctt
     9721 caggtgtata tgaacctctt cgctgttttg catcgaaatt caaacctccg gtagtgaatc
     9781 cccccgccct tattacttcc agcatgcaat atacggcatt atatacatcg gtagggaact
     9841 gatctgtatc ccaccccagc aacatatctc cctgatttgc atcaatggat ccgaacatat
     9901 cgtttattgc cgccgttctt aactcatgct cgaatgtatg tccggcaaga gtggcatgat
     9961 tggcttcgat gtttagtttg aaatgctttt ccaatccgta ttttctaagg aatgatatag
    10021 ttgatgccgc atcaaaatca tactgatgtt ttgtaggctc cttgggcttg ggttctatgt
    10081 agaaagggcc gttgaattta attttattct tgtaatccac ggccatatgc atcatacgtg
    10141 ccaaattatc caactccagc ttcatgtctg tgtttaaaag agtctcatat ccttcacgtc
    10201 cgccccagaa aacatatccc tgtccgtcaa atttcttggt tatgtctata gcctttttta
    10261 tggaagcagc cgcataagcg aaaacatcgg cattaggagc tgtagcggca ccgtgcatga
    10321 atctggggtg accgaacaga ttggccgttc cccataagca tttaatgcct gtgctgtcca
    10381 ttttctcttt gatatagtcg gccattatat ccagattggt atgtgtctcg gccagtgttt
    10441 caccggcagg tgcaatgtcc acatcatgaa aacagaaaaa tccgattcca agtttgctca
    10501 taagcaaaaa acccgcatcg gcttttgcct ttgcatgggc taatgttcct tctttctttg
    10561 aaaaagattt gtcggcggtt cccgaaccga acatgtccac gcctgttccg cacagagtgt
    10621 gccaatagct cattgcaaat cttaagtgtt ctttcatgga ttttccgtga ataacttcat
    10681 tcggattata gaatctgaaa gagaaaggat cggtactttt tgatcccttg aatttaatct
    10741 tcggaatggt ttttaaatag tttttcatat gcctcctacc ttttaataaa attttataaa
    10801 tgcttttaaa aagacatttt tatagtatca gccgcaatgc tctttgtcaa aggttaacat
    10861 atagtgcgaa aaaattctgt ttatgctttc tttatcttaa cgaagctgct atcttcttta
    10921 atgcaggcaa agcatgtgtc agaaaatctt tctgcgggca gtaatcgttt gtttggaagt
    10981 aaaaatacct gtatcttctg catcctccgt tacagtagtc tttaacttcg cagttattac
    11041 atattaggtt atatgaggaa ttgtctttaa gaaatttttt gaagttatcg gaattcaata
    11101 tagagtcaaa atcctgttca tgaacatttc cgccgttaaa atcgtccagt acataaaaat
    11161 cgcacggata gaccgaaccg tcggcttctg tgacaaattg cgcctggcag aatcccgtca
    11221 ttccgcattg ctcgggagga tatcccgcac acatctttac cagattatca aacactctta
    11281 tacttacata ctttccctgc aaatatttct gcaaccatat atcaaagaaa gtttttaaaa
    11341 aatccgcata tgctttcggt tccaaggcat acggttgatt gcaatttgtg ttgtctaaag
    11401 atgaaaggca ggggattaac tgaatgtaat caaatccgtt cttctcataa atattccaaa
    11461 gtttttgagg gtgtcttaca ttgtacttgg ttgcaaccgt cagtatgttg aacggaactt
    11521 catatttttt taacagatta ataccgttca ttactctgtt gaaggtgtct ttttgaaagt
    11581 agtcctttct gagagtgtca tgcgcttctt ttaatccgtc aagcgatacg cccaccagaa
    11641 agtcatgctt tttaaataac ctgcaccatg ccttgtctat aagtaagccg tttgtctgta
    11701 ttgaatacat gtactttact ttttcagtct ttgcctgttg tgcatattcc gtgaaatcaa
    11761 gaaagaaatc taatgttgca agagtgggtt ctccgccctg aaaggaaaag gttatttctc
    11821 cctcgggaca agcctgcagt gcccggtcta ttactttatg tgaagtgtct tgggacatta
    11881 tcccgtaaga ctttatcttt cttttattgc ttacatcgtc ataaaaacaa tatttacaat
    11941 tcatattgca aagcgaagat gccggtttta caagtacatg gaaatttttt ctcatttcat
    12001 tgtccttcaa cttcccggat tttatcacca tgtatgcttt ttggtcaaac gaatatatct
    12061 ctttatatta aaaaaacgcc tcttacctgt gggacaaaaa gacgtttctc atatatgtgg
    12121 agctggtgga cggacttgaa cccccgacct gctgattaca aatcagctgc tctaccagct
    12181 gagctacacc agcatcatgg tgggagcaat agggctcgaa cctatgaccc tctgcttgta
    12241 aggcagatgc tctcccagct gagctatgct cccacgacat gccgttgaga cggtattttg
    12301 aagtatacta aaagcgtaac cggttgtcaa cagggatttt tgaaaaccgg atagccgtat
    12361 tagtaatatt ttatgttgta tacagtttat tgcccttctt ttcggagtac ttaatagcca
    12421 gtccgccttc ggaagtttct ctgtatttac tgaagagatc ccttcccgtt tcatacatgg
    12481 tatccacaac catatcgaaa gatattttcc ttgtatgcgt aagaaaattg gcaagactta
    12541 ccgagttgat tgctctcatg gcggcaacgg catttctttc aatgcaagga atctgtacaa
    12601 gacccattat agggtcgcag gtaagtccca gatggtgctc catcgcaact tccgctgcat
    12661 attcaatctg atccagatcc atgccgaaga gttcggcaag tcccgccgat gccatggaac
    12721 atgccgttcc tatttccgcc tggcatccgc attcggcacc ggatatggaa gcattggttt
    12781 ttattacatt tcctataact cccgccgcgg caagagctct caaaatatcc ctgttggaat
    12841 agccgttttt ttcctgcatg tatttcagta cagcaggtaa caccccgcag gatccgcagg
    12901 taggcgccgt aaccacaata cctccgccgg cattttgttc cgatgccgca aaagcataag
    12961 agcagacaat gcggttttct ctggtggaag gactttcatc catgtgtctc tgtctgtaca
    13021 ggtaccgggc tttccgctga agttttaatc cgccgtgcag tattcccgtg gcattaagtc
    13081 cctctttaat ggacaatttc atatgatgcc aaacatcatc aagataatcg tatatttccg
    13141 gtccttcgca ctgttctaca tattcataaa gctttaagtg gtgcttttca caatattttt
    13201 taatctcggt aaaattcttt tctttataga aatcgggaga gtccatgtat ggtttgccct
    13261 cgattcttat ggcgccgccg cctatgctca tcactctcca cttgtccgct acctcgttat
    13321 ttttaatagc cacaaaatcc atggtgttgg gatgaggcaa atcttcggtg acggtatcta
    13381 tatgtacttt aaccggttta tcggacaaag cctcttttac cgccttgtcg gtcatatgac
    13441 ccaatcccgt ttttgccagc gagccgtaca aagtcacctc aaatctttcg gcatcgggat
    13501 gttcctctcc gaaccttctt gccgccctgt ccggtcccat tgtgtgagag cttgacggac
    13561 ctcttcctat tctgtacagt tcctttaaag acttcattat tattaccttc gcttagaggc
    13621 aattatatca tagatataat ttaataccag tatagtgatg ccttatatat atcggatggt
    13681 atcggtaacg ggaaagcact gtaaaatagc tttaataaag aaaaaatccg aggggagcgg
    13741 atcttttctt gctaagattt gattgctgac atcagagtca gggtacgagc gtattataca
    13801 atatacgaac atttgtttca atagtgaaat ttttactgtc cgattaatta cccagtggtt
    13861 tctctgttgt aaagagagcg gccgatgata tataatttag gggcaggaaa gagggatttg
    13921 tgaagaataa atctgtaata aaaaccatta acataattgt accgattttt attctcggtc
    13981 ttattatctt taactcgaca atagaagcgc tggtgcttgc cgttttttac tttatatata
    14041 ggataataat cggattatcc aagatatatt catatatagc cataaagtat tatgtaaaag
    14101 gtaatttaaa gaaggcaaga aagtactata aaaaagcata tgaagtgaaa gtaaaggaac
    14161 cgcccattgt cgcatcatac gcatacctcc tcatattact cggcgagtat gatcaggccg
    14221 aacaggtgct gcaagtactg gaaagcatga agccggtagg caaagccgcc acatcgctga
    14281 tgctttgtaa tgcggtgctt atgtggaaaa gggataaaaa ccttatcgcg gcacttgcta
    14341 aagtggaatc gatggatgag accatgcgca atcaatggta ttacaacgta tatgccaaac
    14401 tgtgcatttt ttccggtaat attggaaagg caaaggaagc cgccctgaag ggttatgaat
    14461 atcttgagag caatccggta gcggtggaaa atcttctcat tatacattgt ataaatgaag
    14521 agtatgacaa agctctttcc gctgcaaaca aacttataaa caacaaaaaa ttccgggcat
    14581 catcacagga tgcatattac tacagtgcgc tggcttacga aaagaacggc gataaggaaa
    14641 cggcaaaaaa actgtatctg aaggcgttac ggtatgaaaa tacgacaatg acttatgtag
    14701 ataaacagaa agtgctggat aaggcgggag agtatatgaa atgaaagaaa aactgaaggt
    14761 tgcatggcct aagattgctg attacattgt tatgactgta ggggtggtta tttccgctgc
    14821 ggcggtaaat cttttctttg ttccgtacaa gatacactcg ggaggagttt ccggtatagc
    14881 caccgtgctt tattatcttt ttaacaataa ggtgccggtc ggtgttctgg tagcgcttct
    14941 taatctgcct ctttttttaa taggttatag aataatagga aagagattta tgataagaac
    15001 attgtatgcc accgtgatgt acaccgtagc agtcgatgtc accgcaaagt ggttgtcgga
    15061 agtttccaaa aagataacaa ttgaaagcgg taacatacct gatcttatgc tgttttgttt
    15121 cttcggagga gcacttatgg gtctgggatt gggtatagtc tttacccgaa atgcctgcac
    15181 cggcggttcg gatttatttg cggagattat gagacgaaaa ggtatacact acagcatagg
    15241 tacattgatg ttcatgtttg acggcgtatc ggttttgatg tcacttatcg tgttcaaaaa
    15301 tttattactt gtgctctatt cggtaattgc catatttgtt tccatgaagg tgattgacct
    15361 tattcttgac ggcgcaaatg ttgccaaagc cgtctacatc atgtcggata agagcgaaga
    15421 gatagccgac gccattataa acgacatagg tagaggagtc accggatttg ccggtcgggg
    15481 catgtattac aggcaaagca gagagactct gttctgcgtg gttaaaaacc gacagctacc
    15541 cgctttgaag aaattagtta aagaattgga cagcaaggca tttttaatag ttactaccgc
    15601 acatcaggtg ttaggagaag gtttcggcgc ctttgacaaa gagttatgag gtgatataat
    15661 taccgcgtta ggtaataaat gtataacctt cgtaattaca tatataacag taaaaaggag
    15721 ttgttaatgg aacaggaatt attgctaaaa ttgaaaagga aggcagccga tgtcagaaaa
    15781 ggcattattg aagcggtttt ctcagccggc agcggccatc cgggcgggtc attatcctgt
    15841 gcggatatac tgacggttct gtatttcaaa cacatgaatg tcaatccttc caagcccaaa
    15901 gatcccgaca gagacagatt tgtgctttca aaaggacatg cggcaccggt attgtattcg
    15961 gtacttgcgg aaaagggata tatacccaaa gaagaccttt ttacattccg tcaggccgac
    16021 agttatctcg aaggtcaccc ctctttgaaa gatgtaccgg gagtggacat gaccacaggc
    16081 tctctgggac agggaatctg tgcggcatgc ggtatggccc agatgggaaa actggataaa
    16141 aaggattaca gggtttatgc cgtattgggc gacggagaac tgcaggaagg gcaggtatgg
    16201 gaagctatga tgtatgcagc ccactacaaa ctggacaatc ttacggcttt tctggaccat
    16261 aacggtttac agatagacgg agacataaag gaagtaatgt cacccgaacc ggttgttgcc
    16321 aagtttgagg ctttcggctg gagcgttatt ctgattgacg gacatgatat ggatcagata
    16381 gataaggcaa ttaaaaaagc caaaaggacc aaaggtaaac ctaccatgat tgttgcggaa
    16441 acaattaaag gtaagggtgt atcatatatg gaaaataaat atgaatggca cggaaaggct
    16501 cccaatcagg aacaaaggga tatagccatt gccgagctgg atgcgtatat agaaagtctg
    16561 ggggtgtaaa gtaaaatgaa aaaagctaca agagcggctt acggtgaagc gttagcggaa
    16621 tacggaagcg atgaaagaat aatagtactt gatgccgatt tatccaaatc caccatgacg
    16681 gcaaaattta aggaagtata tcccgagcgt ttcttcaata cgggtattgc cgaagccaac
    16741 atgatatcct ccgcagcggg aatagccaca tgcggcaaga tagtatttgc cagttccttt
    16801 gccatattcg cggcggtaag agccgccgag cagataagaa actcggtatg ctatcccaga
    16861 ctcaatgtca agatagccgc aactcatgcg ggtatatcgg taggagagga cggtgcgact
    16921 catcaggcga ttgaggatat agcggtaatg agggcattac ccaacctgac ggtaattcag
    16981 ccgtgtgacg gaagagaaac caaacaggcg gtaaaagcaa taatagatta tgacggaccg
    17041 gtatacttac gtctgggaag gctgcccgta gaagacatat atgataactc atacgaattt
    17101 acctggggta aaggtgtaca acttaaagac ggctgcgatg taacaataat agcaacggga
    17161 cttatggtag ggtccgctct ggaagcttat gagatattaa aggctcaggg tattctggca
    17221 agagtaataa atatacatac cattaagccg attgacaggg aaataattat caaggcggca
    17281 aaagagacgg gagctattgt taccgccgaa gaacatacca taatcggtgg actgggttcc
    17341 gcggtatgtg aagttttaag cgaagagtac cccgttcccg tgaaaagaat aggaattgaa
    17401 gacaaattcg gaaaatccgg aaaacctaat gaacttttaa aactttacgg actgacaccg
    17461 gaagatatag taataaaagc aaagaacgta atgacattaa aaagaaaatg atatttgaaa
    17521 cacacgctca tctgcttgca gaacagtttg accaagacag gaatgaagtt atacaaagag
    17581 ctttagagaa cggcatattg gccgttctca atgtttccga cgacatgaaa tcttccaaga
    17641 aagccgttat gctggcacag aaacatgaaa gagtattcgg ctccgtcgga gtacaccctc
    17701 ataacggagc ggatttcagg gatcaagatg tcagtatact taaggaactt acttccgatg
    17761 agaaaatact tgcaatcggt gagatcggtc tggattatta ctatgacttt tgcgaccgaa
    17821 cggtacaacg ggaggttttt gtaaaacagc tggatttggc aaaacaactg gatttacccg
    17881 tgataattca taacagagaa gcgcataaag ataccattga catattatct tctttcggta
    17941 aagacattaa aggggtaatg cactgctatt cgggaagtgc cgagagtgca aaaatacttc
    18001 ttaacttagg catgtatatt tcctttaccg gagttattac attcaaaaat gctaaaagag
    18061 ccgttgaagc attggaggtc atccctttgg acaggctgct tttagagacc gattgtccgt
    18121 atatgtcacc tgaacctttg agagggacca gaaatgagcc taaaaatctt gtatatattg
    18181 caaggaaaat ggctgaggtc aagaagatat cgtatgaaag acttttggaa caattgtggg
    18241 aaaacagttt cagactgttt aaaaaggcgg agaaatacaa acagacattg gaaacaacat
    18301 gaagaataca gacaacatca tacctcttcc ggattatgta agagcgaaga aagctttggg
    18361 acagaacttc ttaacagaca aatccatagc tttgaaaata atagaagcgc tgaatcttga
    18421 caaagaggat attgtaattg aagtgggtgc gggtaccggt gccataacgg cacttatagc
    18481 tccgttatgt aaaaagcttt atgccgtgga gaccgataaa gaccttataa aaccccttgc
    18541 cgctgttctt gcaaagtatg ataatgtaga aataataaat aaggatattc tgaaaaccga
    18601 tattaacggt attattgata aagaatgcag atataaaaca gtagccaatc ttccgtacta
    18661 tattaccact ccggttatat ctaaatttgt ggaacaagaa aacccgcccg acgtaatggt
    18721 ttttatgatg cagaaggaag ttgcggacag attgtgcgca tcagagggaa gcaggatata
    18781 cggaagcttt acgatatatg tgggttacag atatgaacct tcatttgtag tgaatgttcc
    18841 gccgcaaagt ttcatccccg caccggaagt ggacagtacg gtattgaagt ttataaaacg
    18901 ggaaaaaccg ccggtggttg tgccgtcgga agagttcttc ttttcggtgg taagaacggc
    18961 attttcccaa agaagaaaaa aactttccaa ttgcatagcg gcaatgccta tgtttgatat
    19021 aagcaaaaat gagacggaag atatacttat acagatgggc ataagggcgg atgccagagc
    19081 cgaagctctg tccatggagc aatttgccga gctgtcttgg agattatatg accgaaatac
    19141 agacaatgta aataaatgac agtttaacaa cactattgcg gaagaaaaaa aacaaaattt
    19201 gtttcgttta aaatttaaca tgtaaattct taaatttttg taaaatcgct attgcaaaat
    19261 aaaaagggta gccatatact aataactaac tgttgtgttt atattcacac taaacatact
    19321 atatattggg agagagggtc atgattacga aaattatcaa acgcgatggc cgggaggaaa
    19381 gcttttcgtc ttttagaatc accagagcta tattcctagc cgcagaagcg gtagctaaag
    19441 aagaaaacac caaagccgat ttcacaattg cggaacagtt gaccgagaag gtcgtgcaat
    19501 tgctcaatcg aagattcatt gaaacaattc ctacagttga ggatgttcag gatgcggtaa
    19561 taaaggtact tattgaagag ggtcatgcca aaacctccga gaagtatatt ctgtacaggg
    19621 aagcgcgaag taaagcacgc tccatgaaga cgcgtcttat gaaggctatt gaggagataa
    19681 cctttcatga tgctaatcaa agcaacttaa aaagagaaaa tgccaacata gacggcaaca
    19741 ccgcaatggg caccatgctt cagtacggat ctgcagtgtc aaaggaattc tgcaagacca
    19801 atctgttgga tcctaaacac accaagatgc acgaagaggg agatatccat attcacgata
    19861 tggattttta caatatgggt actctcacat gttgtcagat tgatctttcc cgtctgttca
    19921 aacacggatt ttccacaggt cacgggcatc ttcgagaacc tcaggatata gcctcatata
    19981 ccgcgctggc tgcgatagct atccagagca atcagaacga tcagcacggc ggacagagta
    20041 taccggcatt tgactattat cttgcggacg gtgttgctaa gacattcaga agaacttaca
    20101 gaagcaatac aataaaagcc atgaagatta tttacggaag tgtcgttccg gattgtgagg
    20161 ataagataaa gaattttgag aaggaagtcg gtctgtatgc aaaaatagaa atgcatagcg
    20221 agtatctgaa gaaattcaac gattttcttg taaaagaatg caaagtcaaa accaaagatt
    20281 tagataagat taataagttc gcttacagtg agtctttagc ggaaaccgat aagagaacat
    20341 tccaggctat ggaaggaata gtgcacaatc tcaacaccat gcattcaaga gcgggagcac
    20401 aggtgccgtt ttccagcata aacttcggta ccgacatatc ccccgaggga cgtatggtaa
    20461 ccaagaatct tctgcttgct caggatgcgg gcttaggcaa cggagaaaca ccgatattcc
    20521 ccatactcat attcaaggta aaagaaggag taaactataa tccggaagat ccgaattatg
    20581 atttattcaa gcttgcatgc agagtttccg caaaaaggct gttccccaac ttttcgttta
    20641 tagatgctcc gttcaacatc tcatactata aaccgggcaa ccccgatacc gaagttacat
    20701 acatgggatg ccgtacaagg gtattgggta atataaacgg tgatgaggtg gtaaccagaa
    20761 gaggaaatat ttctttcaca tccataaacc tgccgagact gggtcttaag cacggagtgg
    20821 tgctgaaagg taaattcgat gaagcgggat tctttaagga acttgacgaa aagatggaga
    20881 cggtaaagca gcagcttctg gaaagattgg tagtacaggg cagcaagaaa gttaaaaact
    20941 tcccgttcct catgggtcag catctgtgga tgggatcgga gaatctgggt tgggaggata
    21001 cccttgaaac tgttgtaaaa cacggaaccc tgacaatagg atttatagga cttgccgagt
    21061 gtctgaaagc tctgataggc gaacaccacg gggaaagtga aaaggctcag gaactggggc
    21121 tgaagataat agggcacatg agaaaaagat gtgatgagtt tatggaagaa aacaatttga
    21181 acttctccct tatagccacc cccgctgaag gattatccgg cagattcacg gcactggaca
    21241 agaagattta cggaaccatt gaagggataa ccgacagaga atattacacc aattccttcc
    21301 atatacccgt atactataaa atatccgctt acgataagtt gcaaaaggag gcaccttacc
    21361 atgcacttac caatgcggga cacatatctt atgtggaaat agacggagat ccgttaaaga
    21421 atctggaagc atttgaagat atcataaggt atatgaaaga atgtaatata ggttacggaa
    21481 gtataaatca tccggtagac agagactcga tatgcggtta tacgggtgtt atacaaaatt
    21541 ccgtttgccc caattgtaaa agagaagaaa agaacggccc caagtttgaa agaatacgca
    21601 ggataacggg atatcttgtc ggaacattgg ataacttcaa tgattcaaaa gccgcagagg
    21661 aaagagacag ggtcaaacat gcataagata aggctgtccg gtaagtgcac aagggacagc
    21721 acggtggacg gacccggttt aagaattgtc atatggacac agggatgtct gcataactgt
    21781 aaaggatgcc ataatcccca tactcacgat ttaaacggcg gatttacgga agacatagac
    21841 aaggttatag agtttataaa ccaatccaaa atacaaagag gcatcaccct ttcggggggt
    21901 gagccttttt tgcaaccgga agctttgata cccatcgtaa aagcggcaat agaaaaaggg
    21961 ctcaatgtgt gggcattttc cggctacaca tacgagcagt tgctgaagga tgaaaacaaa
    22021 aaagctcttt tagactatgt cgatgttctt gtagacggcc cctttatatt ggaacagaaa
    22081 gacgagcggc ttacctacaa aggatccaaa aaccagagaa taatagatgt gaaacaatct
    22141 tatgcacaaa acaaggttgt tttaagccga tatgacgagg taaatgcgga tttttagaat
    22201 cttaataaaa tattaatgat attaaggctt tttccttaaa catttatagg gcaaaatagt
    22261 aagtgaagga gacagccatg tacaacatac ttatttgtga tgacgattac gatatagttt
    22321 ccgcattaaa gatttatctt tccggtgata actataatat tttcacggca tataacggaa
    22381 aaaaagcaat agagattata aaagagcaca agatacatct ggtgcttttg gatataatga
    22441 tgccggaagt ggacggtatt accgcaatgg tggagataag aaagataagt aatgtcccca
    22501 ttattctttt gactgcaaaa agtgaagaca atgacaaaat actgggtctg aatttaggag
    22561 cggatgacta tataaccaag ccatttaatc cggtggaagt actggccaga gtaaagtcgc
    22621 agctgagaag atatatgcac ttaggttcac aaaccaagtc acaagccgta ataaggatag
    22681 gagatatcga attggatgat gatgctaaga cggtaacggt atcgggagaa gaggtaagtc
    22741 ttactccgat agagtttgat atattaagac tgctcatgaa aaaccccgga aaagtatatt
    22801 ccactaaaca tatctattcg gaagtatggg atgatgaacc gtacggtgcg gaaaataccg
    22861 ttgccgtaca tgtaagacac ataagagaaa agatagaaat aaatcctgcg gaacccagat
    22921 atttcaaagt ggtctggggg cagggctata agatggaaag gcagaatcaa caatgaagaa
    22981 cattaaatac ggtttgttaa tgaaaattat ggcatatatt cttatcacga taagcgcact
    23041 tgcctttgcg gtttcggttt cggcatcggt ctacattaaa ggaagtgaca tagacagaaa
    23101 tcccggaatg gctttcttcg agagcgaaat atgtaaaaaa ataacccatg attatgcaac
    23161 ggaagtctat aacgggtatt ttaaagagta catgttgaca tatatggact ggcagaaatt
    23221 agaagattat aagcagtatt attcaaaaga aaatacaaac ttcttttttg caataaccga
    23281 taaatgggga aaccatgtgc tttccagtta tacggataca tacggttaca ggaatacttt
    23341 tgttatggag caggacggtt atacggtgac agtttttgta agaacggata taacggcaga
    23401 ggatgattat ctgtttccgt acaagttata tacttttgca cacgaagtaa gacacttatt
    23461 gataccgata gcatcagtct ctttagttgt aacagttctt ctgtttatat atcttatgtg
    23521 ttcagccgca cataaaaaag gcagcgatgc gcttgaaccg tcctttattg ataaaattcc
    23581 gttggactta ttacttgttg ttttcgcagg cttaattata ttggcttatc tattgttcag
    23641 cgagcttgtg acagcattgt attcgatgta catgtttgag gaattgataa tggttttatc
    23701 tttcgggttt gcattcgtgt tcatattatt tatgattttg gctgcaagta ttgccgcaag
    23761 aataaagctt aaaacgctgt ttaagaacac ccttatctac atagtcacaa gagctgtatt
    23821 ccgatttata tggaaaatac tcggcaagtt acctctttta tggaaattgc ttctctttat
    23881 aggagcggta tcacttattg aacttgtagt cattgttgtt ttcacggcaa aaggtggtgc
    23941 tgcacctgca gttatatggt ttcttttaag aatgggggtg acagcttttc tttgttttat
    24001 tactcttaat atgattaaga taaaagaagg cggagaagaa atatcccggg gaaatttcca
    24061 atacaagata gacaacaaac atatgctgac agattttaag agacatgcgg ataatcttaa
    24121 caacatttcc gaaggaatgt ccgatgcaat aaaggaaaaa atgaaatcgg aacgttttaa
    24181 aagtgatctc ataaccaatg tgtcgcatga tataaagaca ccgctgacat ccataatcaa
    24241 ttatgtggat ctgttggaca gacaaaatat tgatgacagt acatcaagag aatatattga
    24301 ggtgcttaaa aaacaaagtg cgagacttaa aaaacttacc gaagatttaa ttgaggcatc
    24361 aaaagccaca tcgggagtta taaaggcaaa tatggaaaag ctcaatctga aagagataat
    24421 gaatcaggta tcaggtgagt attccgcaaa gtttaccgaa aacaacatag aacctgttat
    24481 aaatgatttc ggtgaggata tttatatttg ggcagacgga cgactcatat ggagagtaat
    24541 ggacaatatg atgggaaata tctgcaagca ttcccatccc ggcaccagag catatataac
    24601 agtggaacag caggacaatc ggataaagat ttgttttaag aatgtatcaa aggaaaaatt
    24661 gaacataacc gcggaagagc tcatggaacg ttttgtaagg ggcgattcat ccagaacaag
    24721 tgaaggcagc ggattgggat tgtccatagc aaagagtttg acggaaataa tgcaaggcac
    24781 aatgcaattg gaaatagaag gtgacctttt caaagcctgc cttatattca ataaaggttg
    24841 actattttaa acaacctgaa cgtgggaggt taacggtatg gataacaaca aaaaaagaag
    24901 cgctgtttta tccttcgcat taataatagt tattattgca acggcactga tatcggcatc
    24961 ttgcaataac cctgtattta tccggaattc ctttaacgca acaaccttca acagtgacat
    25021 agttataaaa agaacggacg gtcaggagcc tctgaatatg ccgtacagtt attccaaggc
    25081 tttgctggat aacagattgt tatttgaaga agagattatc ggattcaaca tatcttccgt
    25141 taagtatgag tttagtgata ccggtatgaa tatgtatgct aacagagatg tttttgtaaa
    25201 tgaggacagc agagaagtaa aagctgttat ggattccgta aagtactgta aaggtataac
    25261 aactttgaac ggtattacat ccgacaagga agacagtaaa ataaccgttt atgaaggtta
    25321 ttccgcaaat ctgctgaccg aaagcttacg gaactatgtc attattccct ctacattgag
    25381 ccgtcatatc aataaagaat tacctgataa tgagaagata ctgtttatag taaatcccgg
    25441 tacaaacggt ttggcgtatt ttaccataat aggagaatac acaacaaaaa atgagaagga
    25501 tactctgtat ctgtcctatg agggatttgc taatatggct cgcggagggc aggaagacat
    25561 atccgagcat gttgaatgta tggagattga tgtaaacgaa caggctgacc ttacaaattt
    25621 ctcttttttc ttaagcgcat atttcgccga cataaatgcg ccggatcagt atgagaacag
    25681 aataaacaga cttaatgaac cttatcctta tatgtttgta aacagtgtga acataaagcc
    25741 gatatctttt acggaagaca caaactttaa aaagaacata ataaccgtct ccagaatcga
    25801 caaagaggaa aaacttctga tgtcgtattt atacagcgat gcattgatta aagactatta
    25861 tgaatattca cggtacataa ccgacataaa tataagtacg ggcataaaag gagtgaattg
    25921 gcgggattat ctgacacctg cagattataa ggaatatggc ctttcggtag aaccttttga
    25981 tgaattcggt aaccaatgga gcgattaccg taacaagtcg ggcaagaatt gtgccgacta
    26041 cttttatcaa caggctgtta caagcataag tgagattaaa aaaatgaaaa gcaattgcaa
    26101 gataacattc tactccaact ataccaacag ggaccttgtt gttcagagaa aagaagacta
    26161 taaagaggct gcatttatgg gagaaattat gatggcccct ccgataaaag gatatgcaat
    26221 aatacctgta acaatgtatg aagctgcccg cagtcgtcgc aatacggatc accatattat
    26281 agaaatggcc gaaatcagtg cagcctatct tgcaaggagt gcattgcgtg taggatttaa
    26341 ggtaatagga tattatgagg tgtcggaaga gcaaaaggtt gaggtgccgg acagaaaagc
    26401 ggaggagata atggttgagt tttatgacca taccggtaat ttgattaaga tgcccgtggt
    26461 tgagaaaccc gagtatatat ctgacgggta tgatgtgata tatctgtcat atataggata
    26521 caatgacagg tatgagaggg aactgttcag gaacgagtac atagaatcta taactataga
    26581 aacaagaagt gattccgata tcgctccgct tataagatat ctggaacagt attttgcgcc
    26641 tgcgactgat gttaagaaat atgagaaaga gaaaaatgta ctgggactgg attacgaata
    26701 ttgctataca ataaaggaaa aagctgatta acggttgaag acatagatat tgtcggacaa
    26761 atcatggaca aggcagaagg tttggtatta tttatgtatc aggataatta ggttgacttt
    26821 tgtatcgata gagcatacaa tgtcgatatg atagtcaatt ttgttgggag tgatatatat
    26881 gattatagac aaaataaata agaaaatgaa aaataataac tatattcttt ccactgaaca
    26941 acttacggat atcggtgtat caaagacgac actttcgaat tatgtaagag aaggattatt
    27001 agatagatgc ggacatggct attatacagt gccggattca atagaggacg atatgtattt
    27061 gcttatgctc agatcaaaac atattgtttt ttcccatgaa acagcattat ttctaaacgg
    27121 tttgtcagag cgaacaccgt tttatcattc ggttacaatt cccagtaatg catcgcttcc
    27181 tgcatccgtt aaacagaaat gtaattgcta ctacataagg ccggaactat atacactggg
    27241 tctttacgag aaagtcaata cttttggcaa tatagtgcgg tgctatgatc cggaaagaac
    27301 tatatgcgat atccttcgaa gcagaagtcg gttagatgaa gaaacggttc tttccgcaat
    27361 aaagaattat gcatcttttg gaggtaagga tttgaataag ctgggtaatt ttgcagaagc
    27421 atttagagta tcaaaatcgc ttcgcatata tttagatgtt ctgttatgag cctgacaaaa
    27481 tagacgaaaa ttgaaaggct tatttaaata aaaggaggaa agatgaacta cattatactg
    27541 gatttagaag ctacctgtga gcaaaacaac agagattatc cgaatgaaac tattgagatc
    27601 ggagcggtta agatagatga aaactttaac attgtttcta cttacaatga gtttataaaa
    27661 cctgaaatca atcctattct gactgatttc tgtaagcagt taacaagtat cacacaggaa
    27721 gatatagaca atgcaaaaac ttttcccgac gttatccgcc atttcaagaa ttggataggt
    27781 gtagagacgg aagaatatat tctttgttcc tgggggttct atgacaaaaa gcaatttacc
    27841 agagattgcg aactgcacgg attggattcg tcctggacaa acaggcatat aagcttgaaa
    27901 catcagttcg gtactattcg taatgagaag ccttgcggaa tggaaaaggc attaaacaag
    27961 ttaaacatcc ctttggaggg aacacatcac agagggattg atgatgcaaa gaatatagct
    28021 aaaatatttc ttatgtataa ggataagtgg gtttttactt acgaatagaa acagaggata
    28081 accgtataac atgtttcttg tcctaagaaa catgtttcac agatgaaaaa caaaagtccg
    28141 aaagaaaaac agcgtagcat atatacctgt tgattggtgt ataattaaga agagatacta
    28201 tgacataaga tttatttaag aggtgaagta tttatgccct ttaacattat tcgtcaggac
    28261 ataactaaaa tgaaagttga tgccgtagtc aatgcggcca acacccgact aaaaatgggc
    28321 ggcggtgttt gcggagccat tttcagagct gccggagcag aagcacttca ggctgcttgc
    28381 gacaagcttg ctcctattga aacgggaaaa gccgttataa ctcccggttt tgatttaccc
    28441 gccaagttta tcattcatac ggcaggtcct atatacaaca tgcagaccgc aaaggaaagc
    28501 gagagactct tacgttcctc ctatacggaa tctttacgtc ttgcagtgga atacaaatgc
    28561 gaaagcattg cttttccgct catatccggc ggcatttacg gatatcccaa agaccaagcc
    28621 cttcaggttg cgacctccgc aatacgggat tttctgaaag aacaggatat cgatatatat
    28681 cttgtgctgt ttgataaatc ggcatttgtt ataggcagag aactgttagg ggatgtcaag
    28741 agctatattg acgagcatta tgtcgaaaga cacgaggtaa aaagagaaag gtttttaggt
    28801 gcggaaagtc gtactatgcg caaagcaaaa gcaagcatta atcgctgttt tggtcccatg
    28861 tatgaatccg ctgatgcagc cacaacactg gacgatgtta taggaaacct tgatgagcct
    28921 ttttccgata tgcttcttcg tcttatcgac tccaaaggca tgaccgatgt tgaagtttac
    28981 aaacgggcaa atctggacag aagactgttc tcgaaaataa gaagcggtaa gggttatatg
    29041 cccagcaagc gcaccgctat tgccatagca gtggctttga aactgtcact tgaagaaacc
    29101 gaggccttat tgaagcgtgc gggttatgca ctgtcccatg ccgtcaaatt cgatgttatc
    29161 gtggagtact ttatcataaa cggtaagtat gacattttcc agataaacga ggtattgttt
    29221 gaatatgatc aaccgctttt aggaaattaa aaatgtcgct tcccgggcga ccgagtattt
    29281 taaagaaggt agtatcattg aattgtaaga aacagtttat taaaaaagga ggtcactgaa
    29341 atgaaaaaag gattaactga actggtattt attctcgaca aaagcggttc tatgagcgga
    29401 ctggaaagcg acaccatagg cggtttcaat tccatgcttg caaaacaaaa aaaagtagac
    29461 ggagagtgct ttgttaccac tgtcttattc gacaacgact atgaactcat acacgaccgc
    29521 atagacatca aggcaatcgc ccctattacc gaaaaagagt attatgtcgg aggctctacg
    29581 gctcttattg acgcaatggg caagaccatc aacaagattg acaatgtaca aaaaaacaca
    29641 gccgaagagt atcgtgccga aaaggttatg tttgttatca taacggacgg acaggaaaac
    29701 tccagtaaag agtatactgc cgataaggta agagcattgg tggaaagaca aaaagagaag
    29761 aacggatggg agtttgtatt cctcggagcc aatatcgatg cagtggagac agccgaaaga
    29821 tacggtattt cacgtaacag agcggtagac tatttgtccg acaagaaagg tactgctcta
    29881 aactacagag ttatggcgga tgccgtgact tcgttccgta agtcgggaga ggttgacgac
    29941 agttgctttg aggagatacg taaggatgtg aaaaaaagag gaaagaagaa ataagaaaag
    30001 attgaataaa atctgaaaag cagactcagc aactcttttc agatttgggt attataaaaa
    30061 cagcggttaa tatattttta tgtcagatat aagggaacag gcataaagga tataccgatg
    30121 ttgacggaat gatgaaggag ctgcttgaat gaaatatgca gttaacatac aatgaataat
    30181 gtgtaataat agatattgag aatgatattt taagaatgag agtttattat gaaagatttt
    30241 ttgtccaaag ttctgccttc gtcacttatg gttggcataa tgtatgcaat acttaccgct
    30301 ggcggatatt taagagaaat aagaattggt gattatgata cgccgaaagt tataagagat
    30361 ggtaatttaa gcatgtttaa atcagtaatc attaccaata gtattttact gggtatttta
    30421 ataatcttaa tttcaattac attgattgca attttgacgc ttatgtttaa gaataaagaa
    30481 atcgattaca gagtaaagat tggtattata ttagccgttt ttatcatatt tatgatctca
    30541 tatttctgta ggaatactcc gtatacagaa aatgatgttt atgacactat tgaaagtaaa
    30601 gcagaagatt acataaccga tcttaccaat aaagaatatg ataaaatggt taaaaattat
    30661 gttcttaaca ggaatatgaa caaatttgtc agcaagaata tatattctca agcgatggaa
    30721 agaactataa gagaatatgg tcagataggt aaaatagaag atcctgttaa agtatatatt
    30781 aatgaagaat atactttttt gtcttatcct gtaatacatg gaaatggtca agtagtatat
    30841 tataatattg cctttaacag taaaaatgaa atatccggtt tatttactac tcttatactc
    30901 ccggatagaa ataataactg atttgttacc tttaaaaaca taatatttct tatgtaatgt
    30961 catatgcatt gtatgattaa aacacataca cgaattacat gaatgttccg cctacttaga
    31021 gctatagtat gcctgtaaat tttcatcact tcgaaaaagc cgttttgctt tactttccgt
    31081 ttaacaattc tatctaaccg ttccgctcgg ttattggtag gcggtacatc cggttggtgt
    31141 aagaacagaa tatagaaata tttcttgacc ggaaacaaga cgaatagtat aattcggtta
    31201 gcgtatgcta actgtaatag tttgtattcc tacgcgaata aaagaagaga aggacagaag
    31261 gcttgaatcg aaaggaattg ataaaaaatg cttatcgcct aaccggtagc aataatttct
    31321 atgacggaat gataacatgt tccacattat taggcagggc ggtatctcgt ttggtttggg
    31381 acatgaacaa ggaagactgc acggattatc aaatgcaagc tcttttggga atacccgaga
    31441 atttttccgg aagattgctg gaagttccgg tgggaaccgg tgtaattact atgccttttt
    31501 acaggacact ttccgatgcc gatattactt gtttagatta ttcgccggat atgatgtcgc
    31561 aagctaaagt aagagcgata ggcttgggat tggagaacat tacttttctg caaggagatg
    31621 taggggcttt gccttttcgg gacaactgtt ttgacgcaat actttcactc aacggttttc
    31681 acgcatttcc ggataaggaa gctgcatata atgagacttt tagagtgctg aaaagcggag
    31741 gtgtcttctg cggttgtttc tatgtttccg gctgtcataa gcgaaccgat tggataatac
    31801 gtcacatcta tcagcctatg aagtttttca gtccgcctta tgaaactatg gacagtttga
    31861 gaaaaagact tggcagtatg tatgttgact ttaaaataag caatgttaaa ggaattgcct
    31921 gttttaattg cagaaaggag taattttttg ttttggcaaa caaagacaga aatactttag
    31981 caaccgaaga atattacatg aagacaggag gatacgatgc aggaaaacat aaattggagc
    32041 aagataagtt ctttaatgaa aatagggctg ttaggtgcct ttattatcct tattggagac
    32101 tttcttatag gctggggtgt taaggatttg agtttgcccg gtatagaggg acagatttct
    32161 cagtatctta caatatccga tagcagattt ttttggtcgt caatattggg agttatcggt
    32221 gtacccgttg ccgctgtcgg tcatttcgga atatatcggc ttttgaagcc ttattcaaag
    32281 aaatatgcta agttgtatgc aataggaggc ttgggatttt tagtttttgg aggtgccggc
    32341 gttcatctgt cttctgttgc tgcaggcttt ttctataagt acatggcaac ggcaagtccg
    32401 gatatggcct tagcatcttc tataaaatac ttttgctatt ttcccctacc tctttgtata
    32461 gcacttatgc ttttttggat aataatgggt tatgcacata tacgggcggt agcgggaggg
    32521 ttcagtccgt atcctcgttg gtgctgggtt tttgcaatgc cggcaggtac actgcttata
    32581 acccttataa ctttcttcgg caaccatgcg atagtaaatg ccataactgt aggtgctttc
    32641 agtataggta atatatgggc gctgggcggg catttattga tgcttaataa ggcaaaggaa
    32701 aactatgaca gtaaaggtaa ccgaaatttc taatctgaaa agaaggagtt gttgacatgg
    32761 aaaagataaa gatagaaaag aacagcgttc aggaaacact tattatcccg ctatacggaa
    32821 gaaaaatgtg ttcagaaaaa tttcccgaac tttataccga tatttatgcg gagagattat
    32881 gcaacagaat agattacgat ttttcggaat tggagaaaaa gaacaaatct tttttgtatg
    32941 aattcggctc ccttgaggct gcaatgcggc agttggatat gatatgggag attaaagact
    33001 atttaaaaaa gtatcctaaa gcaaccgttg taaatctcgg ctgcggattg gatgacacgg
    33061 ggaaagcctg cgataacggg ctttgccata ttgtcaatgt ggatttcccc gatgttatat
    33121 ccatccgcaa acaacttatc atagatggag aaagagaaaa aaatattgcc tgcgatttga
    33181 aagactattc atggatggaa caggttgacg gctccgaagg agtaattttc tttgctgccg
    33241 gagtattcca ctatttcaaa accgaagaag taaagaatct ggtgttggaa ctttcaaaaa
    33301 gatttcccga aggttgcctg gtctttgaca gtgtaggaaa gttagggctt aaaataattt
    33361 taaataaagt gttgaaaaat atggatatca acgatgtaga cggatacttt tatatcaaca
    33421 atcctaaaaa ggaattgaat tggtctgaaa atataaaggt tacttcaaaa ggctatatgt
    33481 taggctatta tgatatgaaa agtcccggca ttcgtttttc acatcgcttt tttgcatggt
    33541 tgggtgacaa aatgatgaaa atgtatgtac ataagatgga atttgcagga aaagaataaa
    33601 aagccttttg taagaaaagg atttgcaata ataccgaaca gcggttttat tatacattat
    33661 gttcttcaag ttctactatt ctgacgttct ttgtcagaat ttccgtgtat tgtaccgaat
    33721 agcattttcc ttgtgtttct atattaaaaa tatgcggata aacacttttt atccgtgctt
    33781 cctgattgct gattctgacc cttggacggt tcatagaaac atcgatatga atgttcggat
    33841 cggtgcggta aagcttgact atttctgctc ttattctatc taccgttttc atattagcct
    33901 ccgttttttt taaagtattc ggaaacatcg gttttgtcgt tgtactgctt tcttatgcga
    33961 acaacaatag aaccgtcttc ctgctcaacc gtatcatcaa ttaaatatct tgtctttgaa
    34021 cgttctaaaa cagctatata atgctcgaat tcttcgcgat tgatttttaa tgcatgttct
    34081 ttactgaatc cgtattcagg tttctggaca aatacgagtg tctgaaaaat acacgccgat
    34141 tttactcgtt tcacgataac acctcctttt ggtaccatac attacggcag aaatatgcga
    34201 cctataatgc agagaggtga tcgagctata atacacgaat gatataacgg ctgccgtcgg
    34261 aatatcagct aatgcactct catggtagtt ttgctacaat gtaattttat catgattttt
    34321 taaaagtttc atgcgccggg gacatacctt ttgcaaaaac attactgtta atatatactt
    34381 gatatgcata aaaacattgg agcataacat gaaaacgact aaagtgagaa tttgcaatac
    34441 ggagtatgtg ataaaaggcg atgaatcgga agaatacatc gaaaagatag cattgtttgt
    34501 tgataaaaaa atgaactcca taataaggga gaatcccaag ttaagccttt ctacggctgc
    34561 cgtcttaaca gcggttaatg tctcttacga ttacttcaag gaaagagatc aggctaataa
    34621 gcagaaagat cttatggacg agtttgagaa aaagtccaaa gaccaggtgt cgcgtgtacg
    34681 attactggaa gctaaaatag aaaaacttac acaagccaat agcgaactta aagaaacgct
    34741 taccaagacc gaggagcagc ttaaaaaggc tcaaaacgaa ttcagatgat aaaagtttac
    34801 atagaaaaaa caaggaaaga tgcaaagatt ccggtttatg ccaacagctt tgatgccggg
    34861 gcggatgttt gtgcatgtga ggatgcggtt ctggatataa acagcacaag ggtgataccg
    34921 accggtttga aagttgcaat acccgaaggg tatgagatac aaatccgtcc caggagcgga
    34981 ctttctctga atacccgatt gagagtggcc aacagccccg gaactataga cgccggatac
    35041 agagatgaaa tagggataat aatgcataat acctccgtag tgaatgaagg cgacttttat
    35101 gtaacggaaa agcacaaccg tcagggtgtt tatcatatta aaaaaggcga cagaatagca
    35161 cagatagtgc tgcaacaggt accgaaaata gagtttgttg aaaccgattg tgtcgccgat
    35221 atcgggaaag acagaggtgg aggttttggc tcgacagggg tcagataaag cggtctatac
    35281 aataaagcag gatgccgttt cggagataac ggaaaagaaa tcacgcttca tttcctatgc
    35341 gataaaaaca gagacggaaa aagacgcctt agccgagctg gaaaagataa agtcaaaata
    35401 ccccgatgcc acacatcatg tgtatgccta tgttgtaaac gaccaggggg tggtttatca
    35461 cagatattcc gacgacagag aaccttccgg tacggcggga ctgccgacat tggatgtcct
    35521 gaaaaaaggc aacattgaaa atgtgctcct tgtaaccgta agatacttcg gaggaacact
    35581 tctcggaacg ggaggactga caagaactta cggtttaagt gccaaaaagg ccgtaaaaca
    35641 ggcggggctg gtaaagagag tagactgtgc cgtttttaca ttgactgcgg actattcgtc
    35701 gtacgaaaag atcaagcgct tgttggacga atgtgttgta atggatacgg aattcagaga
    35761 gcttgttacg gtgcgttttg ccgtggagga cttgaaagcg gaagaacgca ttggtagaat
    35821 gaacgagctg cttaataaca ggtgtgtaat tgaacgaaag aataatatag ttttggagat
    35881 atgatatggc aggacattca aaatgggcaa acataaagca caaaaaagaa aaaagcgatg
    35941 ctgcaagagg taaaatattt accaaaatag gccgtgagat aatggttgcg gtaaaagccg
    36001 gaggaccgga ccccgccagc aattcgcgtt tgcgcgatgt tatcgctaaa gcgaaggcca
    36061 ataatgtgcc taacgacact ataaacaaga gcataaagaa agcggcggga gagggagatt
    36121 cggaaaacta cgagcagata acatacgaag gatacggacc caacggtgtt gccgtcatag
    36181 tagaggcaat gaccgataac cgcaacagga ccgcttccga tgtgcgccac tatttcgaca
    36241 aattcggcgg caacctcggt acaaccggct gcgtatccta cctgtttgac agaaaaggcg
    36301 tattgattat agaggaaaag gaaagtattg ataccgagca gcttatgacc gatgctctgg
    36361 agggcggagc ggaggatttc gagcatgaag atgaaatgta tgagataata tgcgatcctg
    36421 cggagttttc ttcattgaga gaagcattgg aacaaaaagg atatgagttt ctggaagcgg
    36481 aagttcagat gcttccgcag acatatgtca cggtagaaga cgacaaggtt gaaagtatgc
    36541 aaaagcttat tgacaatctg gaagaccttg acgatgtgca gaacatatat cataactggg
    36601 aacaaccgga agaataaaaa catagttgta caacacctgt cggtaagtgt ataatagaga
    36661 acaggtgttt gtttttttat gcagaaaaga attaatgttt cagtcaggga tattgcggac
    36721 ttttctctcg catcgggaga tattgaaacc tatgccgcat tcggttacag ggaagcggcg
    36781 ctggacggaa cgcaaaatca tatacgattt cagaaggata tgataaaaca gtacggtgaa
    36841 gattgctttg caaaggaagt atatgtagaa gacgtattgg aaaatgatgt agtgtatatg
    36901 gttgttaccg gtcgaataga cggtgttctt aaaaccgatg attcaaatat ttttgtatat
    36961 gaaataaaga caacttgcaa agatataaac gagataaagc ccgccgacag accggagcat
    37021 ctgggacagg ctcactgtta tgcatatatg tactgcaaaa agaaaaatat tgatgcaacg
    37081 ggtataaggc tggtatatat aaacagagaa aatcaggaca gcattacttt cgagtaccgg
    37141 ctgactttta atcaattgga aagtatatat tccgagctta ccaacagata tcttgactgg
    37201 gtaaccggaa taaccgcatg gaagacaata agaaatcaaa gcatagaaac actggaattt
    37261 ccttttgctt catataggaa ggggcagaaa aaacttatga gcgaggtata caactgcata
    37321 gacgatgccg taaaactttt tgccaccgct ccgaccggta tcggaaagac aatgggagta
    37381 cttttccccg caataaaagc attaggagcg ggccttgtgg acaagcttat gtatctgacg
    37441 gcaaaaaccg taacggcaaa ggtggcggtg gatgcatata aaaaactgga gaaaaacgga
    37501 cttaacttac gaacggtctg cataaccgca aaagataaag tgtgtcccat ggagaaaaaa
    37561 gactgtgatc cggagaaatg ttccagagca aaccactact atataagagc aagggcatgc
    37621 ttgaaagaaa tacttcatga acagtttttt gaccgcgaca taattgcccg gtatgccgat
    37681 gaatacaata tctgtccgtt tgagctgagt ttagatgttt ccggttactg cgacctcata
    37741 atatgcgact acaactatct tttcgatccc agagtccggt tgcaaaggta tttcgattat
    37801 gaaaatgagc agtctttctg ttttatggtg gatgaagcgc ataatcttgt ggagcggggc
    37861 agggagatgt tttcctgctc cataggtata aagcagctgg aggatttcaa agacagcatt
    37921 aaaaaagaat ggaaggcact gagaaaaagc gtaaatgcac tgattaacga aatgaaggag
    37981 ttgcaggccg aacatttttc gaccatgaac caaggtttta tttcacaggc agagttgcct
    38041 ttggttctta cggacagagc caacagatgt gtcaatgaca ttatgatgta cgacctttcc
    38101 aaaatacctg acgataagag agaatctttc ttggaaggta tgtttaatat tctgttcttt
    38161 tcccgtgtcg cacaaatgta tgacagacgg tttaccacac tgtttacctc cgacggcaaa
    38221 gggctggatg tcaaactgat gtgtctggac ccttcctatc ttttgcgaca aagcatggat
    38281 acgggaagat cggcgatatt gttctccgca acgctggagc ctaaaagata ttacatggac
    38341 cttttgggcg gtgacggtaa tatggacagt ttcctgacat tggattcgcc ctttccgaga
    38401 gaaaacttga aagtaaaact ggttacggac atatccacaa agtataaaga ccgggacctg
    38461 tcctatgaga aaatagcaca actgctgaaa aaacagtttt ctcaaaagac cggtaactat
    38521 atggtatttt tcccttccta tgcttacatg agacgcgttg tggagatttt taccgacctc
    38581 tgtcccgata tggatatttt agaacagaaa ccctatatgg atgatgccga gagaagttta
    38641 tttcttgaga ggttcgagaa gttcggtgaa aatacactgg tagcttttgc ggtaatggga
    38701 ggacttttcg gagaaggaat agaccttgtg ggagaaatgc tttccggtgt ggcgataata
    38761 ggtcccggac ttcccatgct gtgtccggaa agggaactta taaagaagta ttttgatgac
    38821 attgatatga acggttttga ctattcttac acatttcccg gaatgaacag ggtgcttcag
    38881 gctgcaggaa gagtaatacg aagcgaaacc gataccggct ttgtgctgct catagattcc
    38941 cgtttcggga cggcaaaata caggtcgcta tttccaaagt ggtggaattg tagtaatatg
    39001 tacaatagtt ttgaagagga cggtttttta tgttaaaaca atacctgtat atattggcga
    39061 tttcagcggt tcccgtaata gagcaaagag gtgcaattcc gttaggaata ctgctgtata
    39121 aattggatcc tgttcttgtt acacttgttt cctatgcggg ttctttgatt cccatgtttt
    39181 ttgtaatcta ctgctttact cccatattta agtggctgag tacaaaaaag attcttaagt
    39241 ggttttacga ttttgtggat aacaaggtgc gaaaaggcaa ggaaaaggtg gaaagatatg
    39301 aggtaatagg acttaccata tttgttgcca tacctcttcc catgaccgga gtgtggaccg
    39361 gcagtgccgt agccgcattc atgcagatga acaaaaagaa ggcttttata agtgtcagca
    39421 taggagccgc catatccgca atagtaataa ccgtaatctg ctatgtagcc cctaagcttt
    39481 taggatattg atttttccat aactaactta tttaactaat cactttattg gtgatataat
    39541 gacctctatg aagattgttg aatataaaga aagcgatata tcggattttt tcccgttaag
    39601 aaaagacata agctgtaaat cggattacgg caggtttctt gtaataggcg gctcacccgg
    39661 catgaccgga gcgttgagat tatgtgtgga tgcctgttac agaagcgggg cgggacttgt
    39721 gtactatacg gctcctaaaa aagacttact gatatacgat cttctcactg tggaaggcat
    39781 tacagtacct aaagaggata ttatttcact tacgggtgat aaagactgta tatgcattgg
    39841 atgcggcttg tataacgatg aagaaaacct gatactgtta aataaagtat tggaaaactc
    39901 gaaatgcaac atagtagccg atgctgcggc gctgaggatg cttgcggaac ataaagaact
    39961 gttgaaacgg cacggcaaca gaatgaccgt gttaccgcat cccggagaaa tgtctcatct
    40021 tacggggttg tcggtagagg atattcaaaa agaaagagta ggcaccgcat atacttttta
    40081 cgagaggtac ggatgcataa cggtgttaaa aggcaacagg accgtggttg ccggaaatga
    40141 ttcggtgtac ataaacaata cgggaaatcc cggaatggcc gttgcaggat cgggggatgt
    40201 tcttgcaggt atggtaacgg catactcggt tatgagtaat gacatgttca aaaatgcctg
    40261 catggcggta tatgtgcacg gcctggcggg agatctggcc gttaaagact tgggcgtgat
    40321 aagtatgatt tcatcggata taataaggta tataccccaa gcgcacaagg aggtggcatg
    40381 tggaaaagcc tgacagagtg tgggtggaaa tagacttgga taacatagca cacaacatcg
    40441 cttcgataag aaagaaaatc ggtgatgtta aagtggccgc cgtggtaaaa gccgacggtt
    40501 acggacacgg agctgtggag attgcccaga ctctttgcga aaacggcgta tatatgctgg
    40561 tggtggcaac tcttgatgaa gggataagtt tacgaagaaa cggcataacc gctcctatat
    40621 tggtgttagg ctatatatgt ccggaaaaga ttgagcaggc gatagacaat aatcttgctg
    40681 caacaattac caattcggat atggcagtaa aaatgtcttc agctgcgaaa gaagcgggaa
    40741 aaacgctgta tgcacacatc aaaatagata ccggaatgca cagggtgggt tttgcatatg
    40801 atgacaagca atctattctt tccgcactgg ctctttgcaa tattcattac agcgggatat
    40861 tctcacattt ttccactgcc gacgaagaag ataaaacata tacaaatatg cagtatgaaa
    40921 gatttagcgg tgttttagac agtgtgaaaa aagcgggtta cgatatagct ttgaagcaca
    40981 tatgtaacag cggagcgata atactgcatc aagacaaata tatggacatg gtgcgaccgg
    41041 gaataatcat atacggtctg tatccttcgc agtattgcaa aaatcatgac atgtcggatt
    41101 tgcggtgcgc tatgagtttc aagtccagaa taatacagat taagaaagtg aaaaaaggtc
    41161 agccgataag ttacggaaac agttatatta ccgagagaga cagtatcatt gccaccattg
    41221 catgcggata tgccgacggc tattccaggg tgctttcaca aaaaacccat gtgtttataa
    41281 acaaccgaag agctccgata gtaggcaata tatgcatgga tatgtgtatg gcggatatta
    41341 ccggactgaa aggtatatcg gttaatgatg aggtagagtt gttcggccct aatataccgg
    41401 tagaacatct ggcgaccatc tgctccacaa taaactacga gtacatttgt aagataggtg
    41461 caagagtacc gagggtctat atcaaagaag gaaatatagt aaagataaac aactaccttg
    41521 tataacattg attgaccgtt attcaggtag ttatgtataa tgtaaaaaga aataaaaccg
    41581 tataaagagg agaacaaaag atgagacagg ataaaaaaac cgcgatagca ataataaccg
    41641 ttgcatttat aatgagcctt atgttctgta ccacggcaca gactgccgat cttgtaattg
    41701 caagtgtcga atatgtggat gcaaagatag cagaggtaag ggctcttatt cagtcttcgg
    41761 gaacgccgtc gggaggcaca acaccggtta cccccgtgga taatgctaaa ataaccgagc
    41821 tggaaacggc tttgggcaac gcgttaaagg aaatggatgc tttaagatcc agaatggatt
    41881 ttcttgatgt aactgtcaga gacatgtcgg ataattacgg catgcaggtt cttcatgttt
    41941 ataaagatga tgtaatatac gcattaggta cttcggagct tatcttgcgg agcggcagcg
    42001 ccatagtaat aggaaatgca aacaacgaag ggttaagcga tgccaccacg ggagaaaatc
    42061 tcaatcccgg agtatcgctg ggtaaagatc actttgtggt aatacccaga ggtgacggca
    42121 gaggcattaa gattacatcg ggtgaaagct atattatgta cagaggttcc ttctcatatg
    42181 tacccgcaaa ctgatatcaa aagttatatg ccggtaaaga tacttttcgg aaacaattgc
    42241 gtctttaaca ataaagacgt atttaagcag acaggaaagt ttccctttat tgtgaccgga
    42301 aaaagcggag ccgtaaaaag cggcgctttt gatgatgtga tatctgtctt aaacggcgga
    42361 ccgtatattc tttatgacag agtggaagaa aatcccaagg tatccgacat ggaagatgct
    42421 gcaattatca gtaaaaaatt ccgttgcgac tatattattg caataggtgg aggttccgct
    42481 ttggatgcgg caaaagccat agcggtgctg gccgttaata acatgagcgg catggatctt
    42541 ttcagcaaga aggcggatgc ggctttacct gtaatagcca taccgacaac cagcgggacc
    42601 ggaagcgagg tcactcctta ttccatactg accgtggatg agtggcagac aaaaaagagt
    42661 tttgccggcg actgcatata tcccaagatt gcactgttag atccgaaata taccttcagt
    42721 ttacctatcg actataccat atccaccgct ttcgatgcgg taagtcatct tttcgaaagt
    42781 tacttttcct taaagagtaa tgaaatgtca aaaatgtatt cgcaagaggg catacgtgct
    42841 tttgcaaaag cagttgatgc aataaaaaac aggaagtttg atgagcagag cagaagtgct
    42901 cttatgtatg cttccatgtt gggcggaata gcaatatcgc ataccggtac caccgcaatg
    42961 catgcaatgg gttattccat gacatatttc cacggactgc cgcacggatt tgccaatctg
    43021 tattttgccg aaccttattt caggcatatg atgaaacagg aagagcaaaa gacaaaaacg
    43081 gttacggatt taatgggtat gaccgatttg cagtcaatga taaacttctt ttcggataac
    43141 ataccaagac cgaaactgaa caaagaacag tgtgagcgct atgcaacgct ggccatgatg
    43201 cagtccagca ttaaagctac accgggcaac ctgacaaaag aggatattgt aaatatgttt
    43261 aaagaggcag gcaggacatg atagtctatt atcacattcc cgaagacaaa gcaaaagacg
    43321 ccatatcttt cgggatgaaa ctttcggaat acggcaattt ccttataaac atcggcaacg
    43381 gaccgaaaaa tgttatcacg gcatatctgt gtcctgcgga tgatataaag agatataaca
    43441 ggtcgggcta tacctgcttg tgtatcgatt ttcccataga aaagacatat ataatggagg
    43501 gcgtctatct ggaaacggaa aatacccagg cactcaaggc ttctttgacc aaagcggcgg
    43561 agtataggct gggaagttac agaaagcctt tggctcttat caccaccacg gtatttgccg
    43621 aaagaataaa ggtactggga aaatacatgg atgtacccat gattgtggat gactcggagg
    43681 aactttatct taaaaatgct ctgctggagt tggaagaggg cgacagcgga tattatgaaa
    43741 aagccttata cggatatctt acggtcacaa aagaagcgca tatgctttat gaaaagaacg
    43801 gatatgccga atatatactg aacaaaagaa aatacatagt aagaaagccg gatggcaagt
    43861 gatgtgcgat gtggaaatca ttaaacttcc atatataaat tccaccaata catatgcaaa
    43921 agataatgca cagtctttca gtaagaaaat tacggtaata caggcggaaa gacagttgca
    43981 gggcagagga cggttaggca gggatttcct atccgataac agcgacggcg catggttttc
    44041 cgtagccatt aaacctgaaa agaacactat atcgccccaa aaggtactgc tgatgcccat
    44101 tctggcggca ggtgaaactg ctgcggcaat agaggatata tacggtataa aagcggggat
    44161 aaaatggcct aacgatatat tgattaacgg taaaaaggta tgcgggatac tgtgtgaaag
    44221 cagggttgtc gataacaatg tacttcttat tgttataggt ataggcataa atgtcaacca
    44281 aaaagcgatg catccggata tagaggatat tgccacatca ctgtttatgg aaaccgctac
    44341 ccaagtttcc gtcgacctgc tgatacaaaa tatatgtgga aatatagtct gctcttatga
    44401 tagaatgaga cgcgggaata cagacggtat aatagataag tggaatgaat actctataat
    44461 gaataatgcg gtcataacct atacgaaagg acaatcgttg cacagcgccg tttccaaagg
    44521 tatagacagg cagggcaggc tgatagccga acaggacgga ttgacaatat atcttgactc
    44581 gagcgagata agtataaaaa gcacaaggag caaataatga acgacaatcg gaataccgct
    44641 tccgcaaatg acggcaaccg gaagaggtcg aacaacaatt attacagaaa gaaaaagaac
    44701 tattcgggtg agcgaaaaca gaacaatttg cccgcacagg tgactgcagg tgaggaaaaa
    44761 gataacagaa aatcttctca aaataaagga tataataatt acaaacgcta taacagggac
    44821 agaagaagaa accgcagcgg ggaagaaacc gttgatgata taaaaaagga tataatgagg
    44881 atagaaaaag aaatccggct ggagattgaa gaaataaaaa tgataagggt gtagagaatg
    44941 cttttagcta ttgatgtggg aaataccaat atcgtaatgg gggtacataa cggggaaaaa
    45001 tataccaatg tgtggcgcct gacgaccaac aaagaaagaa cccctgaaga attcggagtc
    45061 ttctatataa acatgttcaa tgcagccggt ctgaaactgt ccgatgttga tgccattatt
    45121 ctttcttcgg ttgtaccggg aataatgtat acgcttacca acagtctgga aaaatacttt
    45181 aaccggacac ccttgctggt ggaacaggga ataaagaccg gattgaatat aaagatagac
    45241 aatcccagag aactgggggc ggacataata gtaaacttgg tctccgctta tgaaacatat
    45301 aaaggtcccg ctatagtaat tgatttcggt actgcaacca cattcagttg cgtaacggat
    45361 aatttcgagt gccccggcgt ggtaatatgt tcaggcgtca gtcttacagc tgaggctttg
    45421 tacaaaagag cggcaaaact gcctaatgta gatataataa aacctgctaa ggttataggc
    45481 aaaaatacta taacctccat gcagtcggga atagtaaacg gctacctggg gttgatagcg
    45541 ggcatagtaa aagacatgaa aaaggaactt gcggaagact ataaccagaa ttatgaggat
    45601 attaaggtta tagccaccgg aggcctttta agcattatcg ccaaaaacag cgaccagatt
    45661 gactatatag acagtatgct tacactgaac gggcttaaac tggtttatga taaaaacaaa
    45721 tccaagacgg agggaaaggc ataatacgtt tatggcaaaa ggaatgatta tttctttcga
    45781 agggaccgac ggttccggaa agaccactca gataaaggct ttgagccgat atcttgaaga
    45841 aaagggatat atgccggcaa ttttacggga accgggagga acaaggatag gagaggaaat
    45901 tcgctccata ttacttaaca gcgataatac cgaaatggct tccctgacgg aaatgctttt
    45961 atacgcctct tcccgggcac agatgatgga agaaaaagtc gagcctttgc ttaaagaggg
    46021 aaagatagtg atattagaca ggtttttcga ttcctcgctt gcataccaag cctacggaag
    46081 agacttgaat ttcgatgcgg tactgtccat aaaccttgcg gcggtaaaga atatagtccc
    46141 caagattacc ttctttattg atataacacc ggagcagtcg ctaaagagaa gacagaatgc
    46201 taccgcaacc gaccggttgg aaaatgagga tatgtctttt cattacaaag tatatgaggg
    46261 atataaaaaa cttttggaga aattccccga aagaataata aggattgacg gcaccaaaga
    46321 agcaagagaa ataactaatg agataacgtc atatgtagat aatgttttga aggaggatat
    46381 tagatgaagc tgatatttgc aatagtgaat gatgaagatg aattggatgt aatgagagga
    46441 ctgagtcagg aaggttacgg agtcacaaag ctgtgttcca ccggaggatt cttaaaatcg
    46501 ggaaatacca ccttgcttat aggagtggac gatgaaaaac tggaccacgc cattgaaata
    46561 ataaaagcga gatccaagag cagaaagaag atagttaatt cttccatgat aggagcatct
    46621 cagggtgcag ttctgacagg atatccgata gagataacca tgggcggagc aacgattttc
    46681 gtaaccgatg ttgtaaaata cgtcaagagt taaaatggcg gattttgatg atataaaggg
    46741 aagaaaacct ttaaaaaatc aaatatcaaa acttatcaaa aaaggcagga tatgtcacgg
    46801 gtatatattt gagggtcctg tcgggtcggg aaaaaaactt attgcggatg ctttttcccg
    46861 gcttcttttt tgcaagaatc ccgataaact gaaagtatgc ggtcactgtg aaggttgttt
    46921 ggggcacaaa gagcagtata tactgaaaag tgataaatcc atttccgtgg aggatatacg
    46981 cactcttatc aaagatactc aacttctgcc tttgggcagc ggaaataaaa tctacattat
    47041 acatgatgcc gatacaatga ccgaccaggc acaaaacgct tttttaaaag cactggaaga
    47101 gcctccttcc aatgtctatt ttttgttgac ggttaccaat tccgaacagc tgatagatac
    47161 ggtaagatcg cgatgtgtaa ttatttatac gggagtcaac accgagagtg aggttaagga
    47221 ctatctgcaa aacaaatatc ccgataccga tatacaacag ataaactttg ctgcatgttt
    47281 ttccgagggt tgcataggaa gagcggacac acttataacc gatgaaactt tccggcagac
    47341 cagaagtgcc gcggtaagat atctcagtga aataaacacc gatttaataa acaaagacaa
    47401 atcaatagac ataacggttt ttttagaggt attgcaaagt ttattccgtg acgcacttat
    47461 attcaaagaa accgagcaaa cagacagctt gataaatgcc gattttttgg atattataaa
    47521 aagaacggcg gcaaacaaca gcagcgaaag cctgtttaag gcatgcaatt atgtggcaaa
    47581 ggccgcaaaa tacataaaag ataatgtttc cgtaagcata gctataggaa ccatgaatgt
    47641 ggaattaatg gaggaattga atatatgata accgttgtcg gagtaaagtt cagacattcg
    47701 aacaaaatat acagtttcgc tccgggagat tgggagctta atataaatga caaagtaata
    47761 gtggagacaa tcaagggtac ggaaatggga actgttgcat caccgattac tcaggttgag
    47821 caatcgaatg ttgtggcccc tcttaaggag attatccgaa aagcaacaca ggaggatctt
    47881 aacaaagcgg aggagtcaaa aagcaaagaa agcgaagcct ttgccatctg cgagaaaaag
    47941 atagccggtc ataaaatagc catgaaactt gtcggggtgg aggcaacctt cgatatgagt
    48001 aaaattattt tctactttac ggctgacggc agggtggact tcagggaact ggttaaggat
    48061 ctggcatcca cttttcgcac cagaatcgaa ttaagacaga taggcgtacg cgatgaagcc
    48121 aagctcttag gcggtatcgg ggtatgcgga agagttttct gttgcaccag ttacttaaac
    48181 gattttcaaa ccgtttccat aaagatggca aaagaccagg ctatttccct taatcccctt
    48241 aagatatcgg gagtgtgcgg aaggcttatg tgttgcttga agtatgaaca tgaagtatat
    48301 accgacctta taaaaaagac accgggcata ggtgccatag tgcaaacggc cgacggcaag
    48361 ggggaagtca tcgacgtaaa catgcttaag ggcgagctga aggtcaaatt ggacaaaggc
    48421 gaagagatag atataaggca gtacaatgca caggatgtaa cggtactgaa gaagtccagc
    48481 aagtataaag ttctgcccga tgaagaagcg gcacaggata ttaatgaact tacaaagttg
    48541 gaggataatt gatattttga taaagatata ccaattattt taagcccttg atttttatat
    48601 ttcacggcta tataattgct acaagataca aggaggtgta acatgccata cagaattact
    48661 gatgaatgca tcagctgcgg agcttgcgaa gctgaatgtc ccgtagaagc tattactcag
    48721 ggagacgaca tttatgttat agatccggaa ttatgcattt catgcggtgc atgtgctgat
    48781 gtatgccctg tagatgctcc ggtggaggaa taacaggagt caaatactga gaaccggcgg
    48841 tcagaccgcc ctttttcatt ttaaggaaag ataatgacaa atcactacta tacggacaaa
    48901 cagttaactc atgaggacat gtttgagtac aaggccagaa taaatgaggt ggaatttcga
    48961 ttttactccg attccggtgt attttccaaa accggcattg attacggctc caagcttctt
    49021 attgactcgg ttattgccga tatcggggag caggaaaaag gcctttccaa taaagagaga
    49081 ccttcgcttg ccgatttagg ttgcggaata ggggttatag ggatagtact caacagactg
    49141 cttaagataa acaatacgta tctttatgag ataaacgaaa gagcggccga gttggcaaaa
    49201 ataaatgccg ggcagaacag gtccaaaaga acatatgtaa agaaactgga tatagtaaaa
    49261 gaggatatac cggagaaagc ggacatttgt gtgaccaatc cccccataag agcgggtaaa
    49321 gcgacggtgt ttgcttttta tgaaaaagca tatgaaatgc ttaacgaaaa cggtatcttt
    49381 tactgtgtta ttcagaagaa acaagggctg gacagcacaa aaaagaaact tgccgagcta
    49441 ttttccaatc ttgaaactat taacagaagt gccggatatc atattcttaa atgcattaaa
    49501 gataaaaact gatataatct taatatatat acggaggtac acattaaagt atatgcctta
    49561 gtcggtccga gcggaaccgg caaaagttat aaatctacgt tgcttgcttc ggatatgggt
    49621 atcacccata taattgacga cggcttattg ataagtgaaa accgggtcat agccggaaaa
    49681 agcgccaaga aggaagctac caaaatagct tccataaagc gtgccatttt tgatgataac
    49741 gatcatcgca aggaggttat aaaagcgata agagcgggca aggtggattc tttgctgatt
    49801 ttaggaacat cggataagat ggtcaacgag atagcgcaga gactggaact gccttctata
    49861 cagaaaataa taagaattac cgatatatcc gacgaagaag aaattgccgc cgcgaccaga
    49921 agccgtcagg agaagggaaa acacgtaata cccgttccta cttttgagat aaagaaggat
    49981 tttgcagggt atcttttaga cccgttgtat attttccgaa ggagagacaa gacaaggtat
    50041 gagccggacg gtaccaagac catagtaaga cctacatttt cctacttggg aggatacaca
    50101 atatccaaca acactttgtt tcagattgca agttacggat gtaccgaaat aaacggctat
    50161 gagggcatga cccgctttcg tgtcataaac cgaccggaag ggatatctct ggaagtaaat
    50221 ataacggtaa atgtggatta tgatataccg gaaactttgc gacaggtaca gcagaaaata
    50281 tatgatgtgc tggattttac caccgctttg aatatgataa gaattgatgt gacagccaag
    50341 aatatcatga taaacgggga agtatatcct taaatatgag ttttagtatg cttttttaga
    50401 ttatcatata ataaagcttt atttaaccta caaaatataa tataataaaa aagtaggtta
    50461 aacattttaa cctacacatg gaagtatttt tatatattgt aggttatgga ggaacaaatg
    50521 tctatattga aacaagtgtt aattgaagaa ttagaaagaa acaatcattt tatagattct
    50581 gcggaaaaag aaatttctca acttccgatt ggatatatta gtaaaaaaaa tataaataat
    50641 aagacttatt tttattatca gtggcgggaa ggagaaaaaa taaagtctga atatataaaa
    50701 aagaaagatg ttcaaaactt agataagaaa atcaaaagaa gaaaagagtt agaagaagct
    50761 gtacttaaag caaaaaatga gaataaaaga atagagaggg ccctgaaatg attaaaaaca
    50821 aatataatgc atgcactttg ttgtcctctt tatgccttct ttgatttttg ctcttatgtg
    50881 agaaaagcgg aggatgagtt gtttgctaat atatttgcca attctgtttt attctatcaa
    50941 tccccaaaca agtttacact atcggtacgc cggtttactt gacaatatat atgttatgca
    51001 ttaaactgtc gatatatttt gatttatgga gcgtgaaaat ccttaatgga tagtgacagt
    51061 atataccgaa gtatcgcctt aatcggcctc atcgtcgttg ggggtctgtt ggcgggtatg
    51121 gaaacggcat attcatcctg taataagata cggatgaagt ataatgcgga aaacggcgat
    51181 gttaaatctc aaagagtact gaagatatta gaacaatccg ataaatatat ttctactatt
    51241 cttatactgt taaacgtaat acacataacc gcagcttcaa tagctacggt tattgcggtg
    51301 cgctatctgg gcgagatagg ttctttggta tcaacaattg ttcttaccat cctcatattc
    51361 acattcagcg aagttattcc caaaagtatt gctaaagaca atgccgatgc atattcaagg
    51421 acctttgctc cgctgttgag gtttcttatt gtaatattaa ctcctattgc ctatgtgctt
    51481 tccgcagtag gaaggctggt aagaaaactg atgggaaatg cacccaagga acctactatg
    51541 actgaagaag aacttcagag catgatagag acaattgagg aagaaggtgt aattgaggcg
    51601 gataagagcg aacttatcca gtcggcgata gactttctgg atactaccgc tgcggatatt
    51661 cttacaccca gagtggatat tgttgctgtg gatatagact ggagtaaaga aaagatagac
    51721 agtatagtta taaaggaaaa attctccaga ataccggttt atgagaacac tatagataac
    51781 attataggcg ttttacattc aaggaaatat ctcaaaggta tactgaacaa taccgctgtt
    51841 gatgtggcat cggtaatgga tgagccgata ttcgtgcatc agagcctgtt ggttaatacg
    51901 ctttttgaaa tgatgagtgc cagaagagtg catatggtaa tagtaaccga cgattacggc
    51961 ggaaccgccg gtctgattac aatggaagat ataatggaag agttggtagg tgatatatac
    52021 gacgaagacg atatagtgga agaagatttt gtacatctgg atgacggagt actggaagtt
    52081 gtgggcgaat tgcgtatgga tgagcttctg gacgaactgg atattccgtg caatgatgat
    52141 gatatagaac ataaaacggt ttccgcctgg acaatggaac atcttcagaa tatccccgag
    52201 gttggtgatt gctttgagga tatgggttta tcggtatcca tacaagagct ggacggtatg
    52261 aggatagaaa agctgcttgt taaagtaatt gaaaaaaagc acgatgaaaa tccggaagaa
    52321 gaaaataaca gaaagaagca agaggaggta aaccggtaat ggattggctt atttatctcg
    52381 gaataataat acttattatg ctttccttct ttttctccgg cacggagatg gcattttcat
    52441 cacttaacat aggaagaatc cgtctggaag cggaaggcgg ggacaaaaaa aagaaaaaaa
    52501 cctatgagct ggctaagaac ttctcctcgg tactttatac ggtattggta ggtaacaact
    52561 tagttaatat agccatttct tctttgacca cccttattgc tttaaggatt attgagcaaa
    52621 caaatacaac tcttcccgtt cagaccataa cggttatagt taccaccata gtaatcctta
    52681 tattcggtga aattactccc aagatggttg caaacgacaa tcccaacaga tttgccgtga
    52741 atgttacatg gctggttaat gtattcatat tcatcttcaa gcccatagta tttacggtta
    52801 ccaaaatgac ggaaaagctt tccgtgatat ggaaacctaa aaaagaagaa cctacggtta
    52861 ccgaagacga actgttaagt atagtggaga caatagaaga agagggcggg tttaccgaaa
    52921 aagaaagcga ccttatacgg tcggcaatag agttttccga tgacagagca tttgagattc
    52981 ttaccccgag ggtggatgta tttgccatag atataaatga tgagcccgag gatataatac
    53041 gggcggtagt ggatgaggaa tattccagag taccggtttt tgaaggtact atagataata
    53101 tagtaggtat attgagcaca aaaaggctac tgaaggcagc cgtgaaaaaa ggtgcacaga
    53161 ttgatatccg ctccatgtta cgtgaacctt tatatatata taagtccatg gacatatcct
    53221 ccgttatgga agagttcaag gcaaaaagag cacatatggc tgttgttgtc gatgaattcg
    53281 gaggtactat gggcatagtc actctggagg atgttctgga acagctggtc ggggatatat
    53341 gggatgaaac cgacgacatg agtgaggaca cggtggaaac ggcggaaggg acctatgagg
    53401 ttgccgggga tatgttgctg gatgattttc tggagatgat agattttgat gaagaattgg
    53461 aatcggaatc caccacggtc ggcggttttg tgaccgagag gctgggaaga cttcccgtag
    53521 aaaatgacag ggtggagttt gacggtcatg taataaccgt actggaaatg gcaaacttga
    53581 gagtagaaag aataaaagtg gagatgagaa aagatgctga ctgataatat aaagtggtta
    53641 gggcatgccg ccttcagggt ggacggcagc aaaacgatat attttgatcc gttcagaata
    53701 gatgaaggca aaaaagcaga tatcatattg gtttcccata aacaccatga ccattgttcc
    53761 aaagaggata tattaaagat attgaaaccg gatactttga ttgttaccga agcggacagt
    53821 gcggaaattt tacaactgga taatatgata atagtgaaac cgtatgacag tatttccgta
    53881 gaaggaatag aaatacaggc attgcctgca tataacgtga caaaacccca tcaccccaag
    53941 gaaaacggat ggctgggttt tttgattaat attgacggaa aaacaatata ccacgcaggc
    54001 gataccgact gtatagagga aatgcgggat attacgaccg atgtcgcact tatccctata
    54061 tatcccgata acaaatatgt catgaccccc gaagaagcgg cacaggcgac atattaccta
    54121 aacgcaaaag ttgcggttcc catgcattac ggagtattgg gcggttcaaa gggcgatgcc
    54181 ttaactgttg ccaaaaaagg taatcccgca atacagatag ttattatgga aagccggaaa
    54241 aacagctttt agccttttaa tttatgccct gtaaggatat aatcatatat acagacgcaa
    54301 cagattgagg ttattttaac aggcaaaatg agagagcagc aattcggacc tgaaagtact
    54361 cgggataaag catttatgga aaagaaaaga aaaagaaggc aggcatttaa ggaaaaggcg
    54421 gctattattc tgcttaccgt gctttatctt gtaatatctt taaccaatct cggaaataca
    54481 aaggcaccgg taaacggatg gacaccgcag aaagccgggt cagtcgtgta tgttgatttt
    54541 gaaaaggaag aaaccattga gaggataaca tatctttgcg gtttgggtga tacctggtat
    54601 aacgtgacgg cactggactt atactataag gatgcatcgg gatattatgt ctactttact
    54661 accttggaaa gaagagccag tacatttctc aagtggcttt atacggattt ggaacaaccc
    54721 gttaccaccg attgtgtaaa ggttgtttcc ggcatttatc aggataagga agaagattat
    54781 atcagaggga caaggggaga aatcatggag gtatgtttct ttgcttcaga taaagatact
    54841 cctattccca taagttcggt cagaccgacc gatccgcata aagacgaaaa atccgtcaac
    54901 ctgtttgacg aacaggacct gtatgtgtat gaaccttcat atatgaacag cacctatttc
    54961 gacgagatat attttcccag aacggcatat gagtttttaa taaaaaacca aacaatttat
    55021 gaaaatacgc atcctcactt aggtaagata ataatcatgt ggtcctataa actgttcggg
    55081 gtaaatcctt tcgggtacag agccatggga gcaatagcgg gagcgctgat gataccggtt
    55141 atgtatgctc tgggaaagaa gttattcagg aaccattact ttgctatgat aggggcaacg
    55201 cttttagcgt ttgaatgtat gcatttcgtg cagacaagac tgggaacggt ggacagctat
    55261 ctggttcttt tcataatgct taccttttat tttatggcat gttatgtgga tatggatttg
    55321 cggaaaacaa gctacttcaa aacgcttgtg cctttattct tctccggtat cttcttcgga
    55381 tgtgccatat cggtcaaatg gataggcttt tatgcgggag cgggaattgc atttctgttc
    55441 ttcgttaaga tattttcaca atggaaatct ttcggatacg gacgcattgt atacggaggc
    55501 aaagcacttg ccgcaagaaa cagtctgctt acctgcttct tctgcctgat tttctttatc
    55561 gctataccgg cccttatata ttcactgtca tatactccta tctttcagat acagtcggcc
    55621 gctatcgaca atgaaaagtt tacgataatg gaaatggcgg ataacataat tgccagccag
    55681 aagcacatgt ttgaatacca taagggagtg acacaggacc atcctttcaa atccagctgg
    55741 tatacatggc ccatcatgta caagccggtt tattactact cggctcccgt aaaagccgaa
    55801 ggacagtggg gtacaatagt ggcaataggc aatcctctga tatggtggac cggtcttgca
    55861 gcgcttatag ggacggcgat agtgtcgtta agaaggcaag acagaagagg actgttcata
    55921 tttgcaggct atctttccat actgttaccg tgggccgtgg cacccagaag cattaccttc
    55981 ctgtatcatt atttcggatg tgtgcctttt atgattctgg cgattgtgta tatggcttca
    56041 tactttatgg aggacaatcc caaagttaaa aaatatatta atgcatatgt aatattggct
    56101 atccttgtct ttgcggcctt ttatcccgca atgaccggga taaaagtcag cgtcgattat
    56161 atcgagcttt tgagatggct tcccagctgg tggttttaaa tgagagaatt atatgatgtg
    56221 tcggcttcgg aacataatcc gaaatgggag aatatgattt gccgtaaaga gcatatcggc
    56281 aggaaagaga atgatatacg ctcggagttt taccgggact ataccaggat aataaacagc
    56341 agagcttaca gaagacttaa gcaaaagacg caggtgtttt ttgcaacgag gaatgaccat
    56401 gtctgtacca gaatggagca tgtgaataat gtgttatcca tcagttacgg tgccgcaaaa
    56461 tacctcggcc ttaatgaaga acttgtcatg gcgattgccg taggtcatga tctgggacac
    56521 tcgcctttcg gtcacagcgg ggaaagaatt atagacagga taacaaagga atatctgaag
    56581 acggattttt ggcatgagaa aaacagttta agggttattg attacagcga aaccattcag
    56641 aatgcatggg ggcaggaaga aaatctcaac ctgacttatg cggtgaggga cggaatagtt
    56701 acacattgcg gcgaaataga aagaaaacag ctggtaccgc ggacggaata tattgagctg
    56761 gaagacatta agaatgcttc ttcggtttca ccgtatacgt atgaaggctg tcttgtaaag
    56821 gcagcggata taattgcata tctgggcaga gatattgagg atgctttaag gttgaaaata
    56881 cttaattcac aggatatttc ccgtcttgac gaaatactga agcagtacaa aaaagccgat
    56941 gaagccatga acaacagcac cataataaac agccttgtga ccgatttgtg ctttaccagt
    57001 aaaaaggaac acggcatctg tttttccgat gaaggattca tgatacttaa aaaaattaaa
    57061 gcattcaatt atgagtatat ttacaaaaac aaacggttga gaaagtatga ggaatatgcc
    57121 gaacttatta ttcaatcgct gtttgatgtg ctgatgtcct tttacaaaga gggggatact
    57181 cttaactaca taaaaacaga gtattcaacg atttatcccg aactggcatc ttctttttcc
    57241 gaccggctga aaaagtattc aatcatggaa aaccgaccgg ataatcttaa aaacaaaatg
    57301 ctttataatc tttcaagcaa agaagattac atcatagcgg ttatagacta tatatcttcc
    57361 atgaccgaca tgtatgcaaa aaaactgttt gatgagataa catcattcta aagaaactgt
    57421 ttgatgaaat acataattta ctacatatta atcaaaggga acaggaagca atggaaataa
    57481 aagaacaagt cggaaaaagg aaaacaggta taatcttatc ggctgttatt gtagcggcaa
    57541 tcgcagcgct tttaatattc agtaaagtca gcatcaccgt cggagaacaa gctataacag
    57601 taaaagccgg tctggagcgg tcagttatag cttatgagga catagttaaa gtggagaaag
    57661 taagttccat aacggtggga aaaagaataa tgggtgccga cctcataaaa atatacagcg
    57721 ggacattcaa aaatgagcaa tacggcagat acagactgta tatatacaag gatgtaagca
    57781 actatatagt tgtggaacat aaagaaggat ttgcggtgtt caattcagat acaacagagc
    57841 ggaccgacag cctgtatgaa gacatattgg aaaaattaaa aagataagta agtaaacaga
    57901 atcaggaata gtaaatagta aagaggatag taaagagggt aaaagataat acttgaattt
    57961 atttctcaca gttgtaaaat aaagccatta tgtaaagggg atggcttttt ttgaacggtt
    58021 tatacaaaat agcagactcg cacacacata tatatcccga taaaatagcg atgaaggcat
    58081 caaagagcat cggagacttc tacggtattc ctatgttcta taacggaacg gtgaaagaac
    58141 ttctggaaaa cggaagcagg ataaatatta agaagtatct tgtctgctcg gtgtccacca
    58201 cgtcgcaaca ggttattagt ataaatgact tcataatctc acagtgtctg gagcatgaag
    58261 aatttgtagg acttgctgcc atgcaccagg attttaataa tgtagaaaaa gagctggagc
    58321 gggtagcgga gcagggactt aagggtataa aaatgcacca tgattttcag aaagtatatt
    58381 cggatgatga aagattcttt cgaacataca aaaaagccga agaggcgggc atggtatgtc
    58441 ttttacatgc gggcgacgac aggtatgact atacaaggcc taagcgaata atatcggtga
    58501 tggagaaatt cccgaatctg aaatgtatag cggcgcattt cgggggatac aaatgctggg
    58561 aagaggcata caaaacatat aaagggacgg ataatctgta ttttgacaca tcctccagtc
    58621 tgtattcact gaaacccgat gaagcgcggg atataataag ggaattcgga agtgacagat
    58681 ttttattcgg taccgattat cccatgtggg acgcaaaaac cgaactggaa aggtttttat
    58741 ctctcaatct taccgagcgg gaaaacagca gtattcttta tgataatttt ataaatctct
    58801 tcaacctatg aaaacatcgg caatatacgg aagatgatcg gatgccgtgg ttaacggaac
    58861 aacaacgtca tttaccttga tgtcatatga tacgaatata tagtcgattt ttctgtccgg
    58921 tatatcggaa ggaaatgtga aatatttctt gttgcttata gcagcggtat ctttcagtat
    58981 acccgatatg ctcttcaatt gttcttcatg cggttccagg ttcagatctc ccatgaaaac
    59041 aagagatttc cggtgttgcc tgaagatggg tataagtaaa tccaatgcgt ttttcctctc
    59101 accttcagcc aggccgaaat gggtaactat aacatgtaag tctttaccta tatcaattac
    59161 cgaatctatg aaggaacgct gttcataata tacatcctca tcaagaaccg caggctggtc
    59221 cacaagaacg gtatgaacgg ttttaacagg gtatctggaa aacaatgcat tgccctgaaa
    59281 accttctttt atcgctcccg ctttcccgta gtacatatac tcatagttaa gaagtttatt
    59341 tatctcttcg gcctgtttta caaatccgga aaatcctata ttgtaatgga cttcattaag
    59401 agccagaatg tcgggcgcat actcctttat aacctccgca ctttgagcta agtctctttt
    59461 tctgttttct ccgtagctca ttcccgaaca gatattgtat gtcatcaacc ttatatccat
    59521 aataccttac acaaacggca ttgaccgtat gtattcctcc ttattccatt atatacaaaa
    59581 agagatatgt attaaatcaa attaaacggt aaatacggta aatacgtaaa ggttttatct
    59641 tgtagcctac cgttaataat gatacaatta tcggtataca aggaggaaaa ttatgtctct
    59701 gcccattgct ttacagctat attcagtcag acaggacttg gataaagatt tcgaacaggt
    59761 aataaaaaaa gtaaaagagt acggatatga cggcgtggaa cttgcatcgt tgcacggtaa
    59821 agagcccgaa tatgttaaaa agcttctttc ggataccggt ttaactccca tatccgctca
    59881 tgttccgttg gattcgatgt tgaaagatcc ggacggagta ttcggcacat atgctttcat
    59941 aggatgcaaa tatgttgccg ttccctatct tcctgaagag agaagaccgg ggaaacctct
    60001 atttgacaag actttggaac agatggaaca gttgggtaaa aaagctaaag aagcgggatt
    60061 acagcttctt tatcacaacc atgattttga gtttgttaaa ataggagaca ggttgggtct
    60121 ggatgtcata tacgaaacaa tacctgccga tttattacag actcagattg atacatgctg
    60181 ggtaaaagta gcaggacagg atccggcgga gtatataagg aagtacaaag gaagagctcc
    60241 ggttgtgcat cttaaggatt tctacaagca agaaacggac aaagatatta agatgtatga
    60301 attgattggt atcaaaggaa aagaccataa gccttccgac aaaaaggctt tcggatttat
    60361 gcctttggga ttcggacaac aggatatacc tgaaatactg gaagcttcga aagaagccgg
    60421 tgcaagctgg gtagtggtgg agcaggatga gcctcaagaa ggcagaacgg cattggaatg
    60481 tgcaagatta agtattaaat atttaaaaaa ccttatctgg taaaaaagga gggaaatatg
    60541 tcagaggata ttctaaacaa attcaagaag atacacgagg aaaaaaagag taccgatacc
    60601 ggtaaggtgc ttaaaatagg tatcataggt accggttgga tagcggaacc tcatgtcaat
    60661 gcgtacttaa aatgccccga tgcaaagata gtggcatgtg ccgacttgat agacggaaaa
    60721 gccgcggctt tctgtaagaa aatggggtta gaagatgtgc gtctgtacag aagtcacaaa
    60781 gaacttctgg ataatgaaga gttggatgcg gtaagtgttt gcacatataa ctgtactcat
    60841 gcggaatgtg caatatatgc tctagaaaaa ggtgtgcatg tactcttgga gaagcccatg
    60901 tgtgtaacgc tggatgaagc ggttgcaata gtaagggcgg aaaaagcatc gggtaaagtt
    60961 ctctcaatag gttttcagcc gagatttgac gagaacatga agatgattaa aaagatagt
//
LOCUS       DAOSOM010000008        59373 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_562_length_59373_cov_7.071681, whole genome shotgun sequence.
ACCESSION   DAOSOM010000008 DAOSOM010000000
VERSION     DAOSOM010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 59373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 59373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59373
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_562_length_59373_cov_7.071681"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..606)
                     /locus_tag="PLF14_03270"
     CDS             complement(97..606)
                     /locus_tag="PLF14_03270"
                     /inference="COORDINATES: protein motif:HMM:NF019099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HOT70350.1"
                     /translation="MNRTKKITISAIFAALAIALSVAESFIPFSAIAPGVKLGLANVV
                     PLTLLYVFGPAYAISVQAVRVSVAGLLRGNIVTFLFSFFGGMASITICSLIKRQKRVE
                     FSVIGLSVIGAVIHNAAQFFTALVILKNLNIFWYIAVLTIFAVIAGVLTGLISKYTIK
                     ALERYSNRF"
     gene            complement(603..974)
                     /locus_tag="PLF14_03275"
     CDS             complement(603..974)
                     /locus_tag="PLF14_03275"
                     /inference="COORDINATES: protein motif:HMM:NF018685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HOT70351.1"
                     /translation="MKKADIILIVTLICVFLIFLGLHLFNDSKKSDILVIQHDSKVIY
                     RESIHKDNEIQIKDRYGNITNIVTIKDGTAYMSFADCANKDCKHMGKLTPSSKKQIAC
                     LPNKVLVYLISTDSEVDGVTR"
     gene            complement(990..1877)
                     /locus_tag="PLF14_03280"
     CDS             complement(990..1877)
                     /locus_tag="PLF14_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOT70352.1"
                     /translation="MEYKDYYKILGVDKKDDKDTIKKAYRKLAKKFHPDNNPGNKRAE
                     ERFKEINEAYEVLYDDEKRKRYDTMGMNDFSSAGFDPSAFGQQYSYNYGGSSGFSRSN
                     DFSDFFNMFFGDLGMGADVFGQSSGRKSKASSRQFDSEASMKVSIEDALAQREMIVSI
                     NDKKVNLKIPKGIRSGQKIKMKGQGQGGGDLYIKIELQDTPNLTLENDDLIKTVDIYP
                     WQAYFGSVIQVEMPDNTLKVTIPEKIRGGARIRIKEKGYYKKDKTRGDLYIKVNIVNP
                     VRMSKKDEEMYKELSNRFG"
     gene            complement(2064..2342)
                     /locus_tag="PLF14_03285"
     CDS             complement(2064..2342)
                     /locus_tag="PLF14_03285"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HOT70353.1"
                     /translation="MLKTVLTVIYYLLYALSFLVFIRVIASYFGGARFSKYYEVLVRM
                     TEPFLAPLRNFISWLTKGKPLMFDFSFIALYIIVMILQRIILVIQASL"
     gene            complement(2352..2828)
                     /locus_tag="PLF14_03290"
     CDS             complement(2352..2828)
                     /locus_tag="PLF14_03290"
                     /inference="COORDINATES: protein motif:HMM:NF016363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HOT70354.1"
                     /translation="MSVFDKFLTEFLGFEQAAEEKQTIAPVTRDGKTRSTVQINPIVE
                     RIDALDQIATKKVVPMERCTGTLNVMYPGSFEETKRVCDSLKANRAVIVNLEQVEKDL
                     AQRIVDFLSGTVYTLNGAIRKLSDKILVVAPSGYHIDDEKQEPAKVTDTKSRLYAN"
     gene            complement(2872..3543)
                     /locus_tag="PLF14_03295"
     CDS             complement(2872..3543)
                     /locus_tag="PLF14_03295"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOT70355.1"
                     /translation="MTSHNYVAENIYRIKKQLNNDDILIVAVSKTVTSETAALALEVP
                     GVELGENRVQEFLKKYEYFQGKAKIHLIGHLQRNKVKYVVGKTPLIHSVDSKELLDEI
                     QKRSAMSNVVTNVLIQVKIFDEETKFGVDESAVMDLINHNENNPNVLIKGLMTMAPLT
                     DDEQLISQIFGKLHDIYVDISKNTFYNTHMVYLSMGMTNDYMLAAKEGANILRIGSAI
                     FKDIP"
     gene            complement(3554..4813)
                     /locus_tag="PLF14_03300"
     CDS             complement(3554..4813)
                     /locus_tag="PLF14_03300"
                     /inference="COORDINATES: protein motif:HMM:NF024112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOT70356.1"
                     /translation="MKKEREKKQSGKNTGAVIKILIIIAFVLFAILISRMAIRYYSSI
                     VKKDLAYEGRLTDQINISGLVLYDFDSYDTVSDHSLIKIAEEGSRAGVNHKIGVLANV
                     KNPLYEELCDINARITDIQQLYQINTGILSKDIELIDIQIKDNFLSLSALLNENAMAD
                     AVERKNNIDSLYEYKTDLMSGNNTRQLQINALKSRQKELRQAMASDATDIACVNSGYV
                     TYNMYNGQHNHTYDAMYGMYPEDIKGSISCERDYTGNTVIKVATSRKFHIATILSSSD
                     AARIKDKKGLKIRINTGGLDFEVQVTKVITGNSSDGETVVFFETDNMLSSLVSLGRFD
                     GLLVIKEYSGLKVPVSAIKNYKYSSFQQTEIALVKGISVKFVKIDVLYSDGIYAIVQD
                     ADDDYSFKAYDYYILQPDKVSDGDIIN"
     gene            complement(4810..6072)
                     /locus_tag="PLF14_03305"
     CDS             complement(4810..6072)
                     /locus_tag="PLF14_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HOT70357.1"
                     /translation="MIENFDIDNRLIKLADIVQQQCDAQFKRLEDTAETNQVKVTAAF
                     QRNRVSDAYFAETTGYGYSDRGRELLDEVYADIFRTEDALVRESIASGTAAISLAMAS
                     NVRPGDEIIFANGLPYDTLRSTVGITEARGSLAEYGVTHKIIDTKDNKIDYSAVAASL
                     SKKTKMVFVQRSRGYSIKPPVDKEQFETLSTVVKGYNKNIIMFVDNCYGEFVKEQEPT
                     QWGMDMAAGSLIKNPGGGLCKSGGYIVGTKECIDNASARLYAPGLLKEVGATSNKRLM
                     FQGLYMAPYTVKESLKGAVFTALLFEKLGFKSYPKYDELREDIIQLVLFDKSEQLLQF
                     CRGVQAGSPIDSHVTPCPWEMPGYDDKVVMAAGTFVQGASIEFSADAPMRDPYVAYMQ
                     GGLTYTQVKLGVLKACDIMIKKGMIDIR"
     gene            complement(6069..7277)
                     /locus_tag="PLF14_03310"
     CDS             complement(6069..7277)
                     /locus_tag="PLF14_03310"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOT70358.1"
                     /translation="MKKTDTYYIHEPGRVFKAIVRGIVRAGFRAVLNLRSEKSDDIDS
                     LEGATILIGNHLSAIDPFVMGAYVTKRWVHYLTSQSYFEYPALKKILTYLGAIPKRQG
                     MSDIRSTKIALNILKENRAIGLFPEGARTIDGSMLPFNNSAAKLIKTTGSNVVVSYLC
                     GASISLPRWYRGCSGGFRRGGIEHKAYVLLTKEQVSTMSVEQIDLRIREALYYNEYDW
                     QREKKVEFKSRSMAEGMHAILHKCPKCLTDYSMKSKGMFLYCDECDNTAVVDKYGLLN
                     AQTEDDVSFGDMPEWRAWQNTHLEKEVASDDFVMEFPGSLTVKDSYGKVLKEYTDSYL
                     KITKVELIYTYPEGKTVLPFHSITDAVCDYSLRFEINQEQFSYQFTPLSGQAVIKITD
                     AIKCIKGILR"
     gene            complement(7387..7980)
                     /locus_tag="PLF14_03315"
     CDS             complement(7387..7980)
                     /locus_tag="PLF14_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005338852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiEi antitoxin N-terminal domain-containing
                     protein"
                     /protein_id="HOT70359.1"
                     /translation="MGKKEKIDKLLNTLENGIITASQVSEAGLHRSVLQNLVESGEIY
                     RYGRGIYVKSNAWEDDLYLLQCKYGRGIYSHDTALYLLGYSDRTPAKYTMTFPKGYNA
                     PSLHKENIIVKRVIPKNYQSGIIEIESLSGNPIRVYDLERTLCDILRGSGSDIQIVGE
                     AMKRYATSKDRNIHKLMQYAKQLRVKPKVLRYMEVLL"
     gene            8649..9311
                     /locus_tag="PLF14_03320"
     CDS             8649..9311
                     /locus_tag="PLF14_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013487255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOT70360.1"
                     /translation="MRYTLCLFDLDGTLTDPKIGITKSFQYALSYFGIHEELDNLTNF
                     IGPPLRESFKNSYGFSDTDTEKAVAKYREYFTATGLYENAVYPKIPETLEKLKNNGCI
                     LAVATSKVTVYAKKILKHFNLDGYFALVSGDEMDGSLTKNGKHALINIALDRLDPERT
                     LSVVMIGDRKDDIIGANNAGIDSIGVLWGYGTVEELETANATKIVHTPDELYSLIIEE
                     YR"
     gene            9417..10331
                     /locus_tag="PLF14_03325"
     CDS             9417..10331
                     /locus_tag="PLF14_03325"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOT70361.1"
                     /translation="MNKNKHDIYIELEVQPYSHGRRLSSFLRKEMGISSNCIRRAKTF
                     NNIFVDGQSEHTDYILSSGQTVGITVPDKSTKSVILPADMNLNILYDDDYLIAVDKPY
                     GVLCHPIMDIDEDTVANGLKHIYPNMGIHPVSRLDRDTTGVVLFAKNSVVHHKMSITD
                     IDKRYIGMVIGIPPLDETTIDMPIARVPDTPMERHFSPTGKQSVTHYEVLYKGYNASI
                     LSFRLETGRTHQIRVHCKESGFPIIHDTLYGYQCEYDRYISRQALHSCSVSFIHPFTN
                     EYITITSPLPEDMAGLEKAIKEKSLVND"
     gene            10355..11083
                     /gene="recO"
                     /locus_tag="PLF14_03330"
     CDS             10355..11083
                     /gene="recO"
                     /locus_tag="PLF14_03330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOT70362.1"
                     /translation="MEYLKLKGIVCKITEYKEADKILTLLTYEKGKVQVIAKGVKKKG
                     AALSHAARLFYCGEFECVCKNNMPILTGAAMIQDFFGLTNNIETLYYASHFLDVCSCF
                     IQAEQPSGDIMSLLLNSLYLLMKSNSDPLLLTCILHFRMSAIEGAAPVTDFCVECNKP
                     LDDNCTLGFSLEQDGVTCCKNGKGISGAVYQAINHICTADSSKIFFLSIPKTDLPVLY
                     ELSCKYIEKIAGRHFEILDRIEEL"
     gene            complement(11097..11996)
                     /gene="era"
                     /locus_tag="PLF14_03335"
     CDS             complement(11097..11996)
                     /gene="era"
                     /locus_tag="PLF14_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOT70363.1"
                     /translation="MGFKSGFVTVIGRPNVGKSTLINALTEEKISIVTPKPQTTRKNI
                     KAIYTDDFCQMVFCDTPGIHEPKTRLGEYMVNATKSAFEETDVIIFITALQKDEKIPK
                     EDVHILQLLSEVKNIPKIFVLNKCDTIDQQTAVSIIALYTEKAAFDEVIAISAKDGFN
                     IKSLKTLIIDNLPEGPMYYDKDQITDQTMREMAEEMIREKMLYFLSEEVPHGVAVEVE
                     NFRQRPDMQLYDIDAIIYCEKETHKGIIIGKGGAMLKKIATQARKSMEYSFDCKVNLK
                     LWIKVKSDWRNDSRMLNKLGYKF"
     gene            complement(11998..12408)
                     /gene="cdd"
                     /locus_tag="PLF14_03340"
     CDS             complement(11998..12408)
                     /gene="cdd"
                     /locus_tag="PLF14_03340"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOT70364.1"
                     /translation="MKDKYEELKQKAKEAVKNAYSPYSDIKIGAAVLASSGKIYSGAN
                     IENSSYGASICAERTAIVKAISEKEQSVTAIAITGDVFDYAYPCGICRQVMSEFMQED
                     APVFVSGNNLEWKRYTLKELLPNAFDIKAWKKEE"
     gene            complement(12398..12889)
                     /gene="ybeY"
                     /locus_tag="PLF14_03345"
     CDS             complement(12398..12889)
                     /gene="ybeY"
                     /locus_tag="PLF14_03345"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOT70365.1"
                     /translation="MKTRIDIEGPDEVTALFSRPVKEVSESILARHETDDKDITVSFY
                     LEFVDDEYMLEVNKTYRNMDKTTDVLSFPALNCKEGTIIYNKCDIDYENNELFLGDIL
                     IATSKIYSQAESYNHSPLRECVFLASHGLLHLLGYDHEDKRSEEVMLAIQEEVLKELN
                     YER"
     gene            complement(12873..15026)
                     /locus_tag="PLF14_03350"
     CDS             complement(12873..15026)
                     /locus_tag="PLF14_03350"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOT70366.1"
                     /translation="MNKTKAAKRDLFKTKLYSVVILLLAIMVALLGILQKVSDAGHLI
                     DRHNIATTDIVASHDYYDKEATIAKANKAKDSVEPIAVRINSSQVALYENIFTFFDVT
                     NRYREKTEQLFLTSQQYNAEIDGLVKQYTGMLPSSINISHNLAKAILQDVNSTSYDSY
                     KTVIRAQFSVFMDKYIFYENLAQHQEEFEVAVNQSVTDNNLKELTNVLKSYLIQINSR
                     IDTGATEKERQRVYDDVITNSPVLIMEGAVLYQKGEKLENEDILALYERGMIAASDEM
                     YMYIGATVLFLLCLLASFASVKIYGQTVRLNPKSIFIMVILFFMVYIPALYIPHEYYM
                     YIPYFLLPMMLSILVSDNISFIFSGPMAIFYCYIVGGDLIYLAILLIGSIATIYFVRK
                     TSARIRLAISGIALGVINVALYMSYKLITSDVTISIPYPEIIALFVASVVSSLLCLGL
                     LPVFEVFFNIVTPFKLMELASPTKPLLKRLMMEAPGTYHHSLMVGNLAEEAAEAIGAN
                     GLLARVGAYYHDIGKLNRPAFFGENQSSKTGNPHNTITPQLSATIITGHTTEGADLAK
                     QAKLPPSIRDIIVEHHGTTLVEYFYHKAKNASDEPVIEEDFRYSGPKPRTKESACVML
                     ADACEATIRSMENTNIIEIEKTIRRVAKTKRDDGQFDQCDLTLKELDEILFAFMKVFT
                     GYFHKRIKYPNQQSDDAVKSMGLDRKEDDRDEDED"
     gene            complement(15023..15985)
                     /locus_tag="PLF14_03355"
     CDS             complement(15023..15985)
                     /locus_tag="PLF14_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOT70367.1"
                     /translation="MERLIYFENLSDMMSVFGSSDSNLNIIENMMNVSVIVRGSEVKL
                     KGEAEEVNATEKLINMLIEMAQSGEDINKQNIVYLADLVKNGKDEIYQLPTGYICLNA
                     SGAPIKAKTLGQKKYTDTIRKNGITFGIGPAGTGKTFLAVACAVTAFRAKAVKRIILT
                     RPAVEAGEKLGFLPGDLQSKIDPYLRPLYDALFELMGPDNVARNKEKGLIEIIPLAYM
                     RGRTLDDAFIILDEAQNTTQEQMKMFLTRMGFGSKMVINGDITQIDLPRDKKSGLITA
                     VQILKNIEDIGIVNLTFKDVVRHELVQKIINAYERAESKKGERK"
     gene            complement(16071..16457)
                     /locus_tag="PLF14_03360"
     CDS             complement(16071..16457)
                     /locus_tag="PLF14_03360"
                     /inference="COORDINATES: protein motif:HMM:NF014762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOT70368.1"
                     /translation="MSGLTESMENYLRCIHELSYTNKGVRLTDLADKMKVRKASANNA
                     VMKLSKLGYCAQVKYGPIFLTDAGIEAVKAATGKHLIIVKFLKDILMVDEETAEAETT
                     KIEHHLSTSTVYAMHQLHSKLKNRLD"
     gene            complement(16607..17605)
                     /gene="trpS"
                     /locus_tag="PLF14_03365"
     CDS             complement(16607..17605)
                     /gene="trpS"
                     /locus_tag="PLF14_03365"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOT70369.1"
                     /translation="MKDIVLTGDRPTGPLHIGHYVGSLKKRVEIQEEYDKPFIMIADT
                     QALTDNAHNPKKVSENVYEVAFDYLAVGLKPELNTFFIQSLVPELAEFTVFYMNFVTL
                     ARLKRNPTVKDEMQQKGFGYNVPVGFLNYPISQVADITAFKANLVPVGDDQLPMLEQT
                     NEVVRHINNHFNTAVLRECKPLLSKVLRLPGTDGLSKMGKSMNNAIFLNDEPDVISAK
                     VMSMYTDPNHIRVEDPGNVEINTVFKYLDAFDPEQEELKELKEHYKRGGLGDVKLKKR
                     LNNILQNTLEPIRQRRKEYEENPEEVMKLLKTGSEYARSVAAKTLKELKEVFTLNY"
     gene            complement(17676..18788)
                     /gene="prfB"
                     /locus_tag="PLF14_03370"
     CDS             complement(17676..18788)
                     /gene="prfB"
                     /locus_tag="PLF14_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOT70370.1"
                     /translation="MIDINDIRNQLEEIKEQSDKLILSLDSDSTREEIAALEKETIKE
                     DFWQDNENSQKVLQRIKVLKDRAAKAADLQTKLDDCFILLEFIDEHPEDEELSNELIE
                     AMEQLNDTYTEIRMYSLLNGTNDKNNAYLTLHAGAGGTEACDWAEMLFRMYTRYFEQK
                     GFKATVTDYLDGDGAGIKSVSVFVEGEYAYGYLKGESGVHRLVRISPFDSAGRRHTSF
                     ASCEVIPEISDEIEVKIKAEDLSVDTFRSSGKGGQHVNKTDSAVRITHLPTNVVVACQ
                     SERSQHQNRDKAMKMLMSKLMEIKEREHKEKIEDLKGNQMDIGWGSQIRSYVFCPYTM
                     VKDHRTDYETGDVQSVMDGNLDPFINEYLTQNPAID"
     gene            complement(18788..19954)
                     /locus_tag="PLF14_03375"
     CDS             complement(18788..19954)
                     /locus_tag="PLF14_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT70371.1"
                     /translation="MKKPGEFIREEVRNTPPSGIRKYFDMLSGLSDVISLGVGEPDFV
                     TPWQIRESAIYSLEKGRTHYTSNTGLLELRQAVCNMLRKKYNLDYNPLNQIIITVGAS
                     EAIDIAIRSIAGPGDEVIIPEPCFVAYKGCVNFAGATAVPLQLKEENRFKLTVEQLKE
                     CVTKKTKALILAYPNNPTGAIMTKEELRPIADYLSTKDIVVISDEIYADLTYGTKHFS
                     FAELENMHDKVIYVNGFSKSYSMTGWRLGYVCAHKDIVAEMLKIHQYAIMCAPSFVQY
                     AAIDALEQCEADVERMRKQYDARRRYIYNGLIEIGIDCFLPEGAFYVFPNIKKTGLTS
                     ERFCDRLLMEHKVLAVPGTAFSSAGEGYFRATYASSLENIKEALKRMERFVNTL"
     gene            complement(19951..20427)
                     /locus_tag="PLF14_03380"
     CDS             complement(19951..20427)
                     /locus_tag="PLF14_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOT70372.1"
                     /translation="MDILELLKQDARLTPKELAVMTGKSVEEVEKVIKQYERDKVIAG
                     YSAVINWEKARDNIVVAFIEIKVTPERDYGFEEIAKRIYSYEQVKTCYLMSGAFDIAA
                     VVEGSDLKEVALFVAQKLSAIQGVTATSTHFVLKKYKENDLIFEEEEKDRREAIVL"
     gene            complement(20523..20960)
                     /locus_tag="PLF14_03385"
     CDS             complement(20523..20960)
                     /locus_tag="PLF14_03385"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HOT70373.1"
                     /translation="MAKKRFRNAMSGYNKDEVNKYIDNMMEQYEAKIAEKEATIEELS
                     KKAAELQLAYDELKSKEDALVKEKAGITKALIKANEMSDQIIKEAKEQAIKEVGELEV
                     RAEEEREKIVDIKRQLATLQASAAKLLEKFVENLDKTIGSDEK"
     gene            complement(21033..21920)
                     /locus_tag="PLF14_03390"
     CDS             complement(21033..21920)
                     /locus_tag="PLF14_03390"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOT70374.1"
                     /translation="MKKAAVFSDIHGNLDMLQKAIDICKAQDVEQYIVLGDMISDGPD
                     SQAVLDTVMNLTEHVVRGNREEYILNYHAGKRKQWDNNYQCESLVKTHKNLTDAGLKY
                     ISGLPDMKRLNLFGLDTLIVHGSHISVGHFILPCWNIDVFMDMHDRYGCSLYLLGHSH
                     QSYHFDLMGSFFINPGPLGIPPAISVYKREDAFSMGIISIQDKQLVEYRPVYIPYNPA
                     KLKEYYLKDIDSYEHTFWMELIADSFFTKQYDSINFLHMAKQMAKEKGYENVDPIPND
                     IWEDASNKWRKEHKGTGIF"
     gene            complement(21917..22822)
                     /locus_tag="PLF14_03395"
     CDS             complement(21917..22822)
                     /locus_tag="PLF14_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009609971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70375.1"
                     /translation="MSVRIEKVPYGGWDNCYRIYNDEIELIVTSDVGPRIIRYGFIGK
                     ENVLCEKADQMGKTGGDEWRIYGGHRLWHSPEDKVRTYFPDNEAVDVKIIQNGIRISQ
                     PAETTTMIKKDMEITVKDKGTDVYINHILTNCNLWDIELAAWALTVVAPGGLEVVPQE
                     TLDTGLLPNRMISLWPYTDMNDKRVRWGSKYIFLAHDSKAKKAFKIGISNTDGWAAYF
                     NRNNMFVKKFSPKEDMLYPDYGGSMYETYTCDFMTEMETLSPLVLLGPKEKIEHEELW
                     QLFDNIAPPKDEKEMDKIAKEYITG"
     gene            complement(22906..23274)
                     /locus_tag="PLF14_03400"
     CDS             complement(22906..23274)
                     /locus_tag="PLF14_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70376.1"
                     /translation="MNQQETDNVNKEQQDNCFEPECKPNKWYHRRDAATFPYPVLATI
                     VFLLMGFIWGKWHPGWIVFLTVPVYYAVVNAIKFRTISFSLIIVLIYLAMGFIWDLWH
                     PGWIIFLVIPIIHWFTGKKK"
     gene            complement(23324..24541)
                     /gene="trpB"
                     /locus_tag="PLF14_03405"
     CDS             complement(23324..24541)
                     /gene="trpB"
                     /locus_tag="PLF14_03405"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019879889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOT70377.1"
                     /translation="MDFTNYTKYYPDKNGRYGQYGGAYLPPELMEAFKEIDQAYMTIA
                     HSASFINELRRIRREFQGRPTPVYHCERLSNKLGRCQIYLKREDLNHTGAHKLNHCMG
                     EGLLAKYMGKKRLIAETGAGQHGVALATAAAFFGLECEIHMGEVDIAKQAPNVTRMKI
                     LGAKIVPVTHGLKTLKEAVDSAFESYMKNYKDSIYCIGSVVGPHPFPMMVRDFQMIVG
                     REAREQYLEMTGEHPDEVCACVGGGSNSMGMFTAFLNDPVKIVGIEPMGRGPKVGDNA
                     ASITFGSKGVLHGFESLLLQDEKGEALPVYSIASGLDYPAVGPEHAFLHDMKRVEYTS
                     VTDDEAIEAFFMLSRYEGIIPALESAHAVAYAIKSARQNSANSILVNLSGRGDKDIDY
                     VVEHYGVGDQYKI"
     gene            complement(24562..25602)
                     /locus_tag="PLF14_03410"
     CDS             complement(24562..25602)
                     /locus_tag="PLF14_03410"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOT70378.1"
                     /translation="MYEKNIKEMIEMSRKVGSMPDYTQGGGGNTSVKLDDELMAVKAS
                     GCRLCDITATDGYVVVDYKSVRHYFDTVDLSEKRDFYKESSQFVKDNMVKLEGLNEGR
                     PSIEAGFHSLLEKYVIHTHSVYSNLLCCSRQGESIAKQLFEKSPYRYLWLPYIAPGFY
                     LTLEIQKKIKTMGCIPSVIFMGSHGVIITGKTMAEAEKINEYVSDRIKERLNITKPYG
                     NVAVKQLSEGVYQSDTPRLISYLKGKDYTDEWFDSNILYPDQAAYLLGAITTRGEEKK
                     ARINLKTGITRYHVSYKEALTLEESICAFLFIMDQACKNNLDIYTMEDEDIAFIMGWE
                     GEAYRKAMLNKK"
     gene            complement(25657..27135)
                     /locus_tag="PLF14_03415"
     CDS             complement(25657..27135)
                     /locus_tag="PLF14_03415"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HOT70379.1"
                     /translation="MIKVLAYDFGASSGRAILGVYDGKKIHIEELHRFSNDPVNLNGT
                     LYWDILRLFHEMKVGLIEVKKAGHGDIESIGIDTWGVDFGLIDKDGRLLENPVHYRDK
                     RTDGIMEEVWDKVEKRHLYERTGTQFMQFNTLYQLYSLVKKRPELLKRADRLLFMPDL
                     LSYFLTGKKVAEFSMASTAQMLNTRTGDWDYELLEKLGIPVRILEKVTDSATVIGKLS
                     EQVCNETGLQPITVTAVAGHDTGSAMIATPADTDLFAFLSSGTWSLLGSELPKPIVND
                     LSYKHEYSNEGGINRTARLIKNIMGLWIYQECRRQWNKEGNLASFDELEAAARKAKPF
                     ACLIDVDHQDFYPPGDMPDRVADFCRRTGQYVPKNKAEIVRCIMESLALKYRYAFDGL
                     CEIVGSRIPKLHMVGGGCKNAMISQFTADALGIEVTAGPVEATAVGNIAAQLIALGKI
                     KDLKQARIIIKESFEVKSYFPENRELWEEKYDYYLKNILNKG"
     gene            complement(27174..27905)
                     /locus_tag="PLF14_03420"
     CDS             complement(27174..27905)
                     /locus_tag="PLF14_03420"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOT70380.1"
                     /translation="MSYQELCRKRDYVNGKLPELSPEKIEELEKQFECDYAYESIKIK
                     GSELSRDQVMAILNDKADDMEKTKDVQGTINHSRAFKYMKMYLKKNAYLDEEAIKEIN
                     AIVVEDISVGGIYRNVNLIIPGRKHVPPSPEKAYYKMKKYILDLPYLQYDTVTEKAIY
                     AYAMFMKIHPFIDGNSRTGKLIMNYVLMKEGFLPIYIPQNRYIEYVRAFELFASEDNL
                     EPLAKLAFEFENAQLDIYIKAIDKA"
     gene            complement(27923..29143)
                     /locus_tag="PLF14_03425"
     CDS             complement(27923..29143)
                     /locus_tag="PLF14_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOT70381.1"
                     /translation="MNEFFLLQIENLNNPKKAERLNSLSVLKNAVDKGDMPKPVFYND
                     VNNHIHTAYSFSPYSPSKALFMAYMNGLKTAGIMDHDSVAGCKEFIEAGKILDMPITC
                     GIEVRARMDKTPLNGLRINNPDQDSVAYVALHGIPHQYIDTIQDFMAPYRAARNDRNR
                     AMCDKINAVLNKYDISLDFDKEVAPLSLYHEGGTVTERHISYALALKMIERFKRGKAL
                     IDFYKTTLNLPLSLKNEALLSDVSNPVYEYDLLGAIKSDLISLFYIDAYDECPDIHDV
                     ISLSRKTKAISAYAYLGDVASSVTGDKKPQKFEDSYLDKLFEVLKQLDFDAVTYMPSR
                     NTKEQLIRVRTLCERYGFFQISGEDINSPRQSFVCKAMRDSFFDNLRESTYALINHER
                     QATEDITKAMFYNK"
     gene            complement(29146..29922)
                     /gene="srlD"
                     /locus_tag="PLF14_03430"
     CDS             complement(29146..29922)
                     /gene="srlD"
                     /locus_tag="PLF14_03430"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HOT70382.1"
                     /translation="MKLLNNKVAVITGAAQGLGEALAQRLDKEGCSIVLADLNYEKVC
                     EVAKPLKKAVACKVDVTKETEVDAMTQLAVDTFGRIDIFVNNAGIVISGPTDEFDVNK
                     WRLVQDVNLWGYFICAKSAVKHMLKNKNGASFIQINSKSGKKGSFRNSAYAASKFGGI
                     GLTQSLALEYAKDNIRFNAICPGNLLNSPLWVDSLFKQYAKNQGITEEEVRQKYLDQV
                     PMGRSCEYEDVANVMVFLASDMSGYMTGQAINVTGGQEMR"
     gene            complement(30015..30152)
                     /locus_tag="PLF14_03435"
     CDS             complement(30015..30152)
                     /locus_tag="PLF14_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70383.1"
                     /translation="MSDKDDKDVGQGKQEFRRKIIKTVILVILIIALSALLIYWYFLR
                     Q"
     gene            complement(30145..31626)
                     /locus_tag="PLF14_03440"
     CDS             complement(30145..31626)
                     /locus_tag="PLF14_03440"
                     /inference="COORDINATES: protein motif:HMM:NF022525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HOT70384.1"
                     /translation="MIDFNVSKSRYVRAVQCIKMYWMDRVKPEEFDDSVMDEAVLETG
                     NEVGELAQKLFDNVSVVEYSDDKLQMINRTSEYIRLGKRYIAEASFAYKGRFLSVDIL
                     EVCDDGVIINEVKSSTSVKDVYLDDAAYQSYVLSLSGFNVKRVNVITLNSSYVRKGEL
                     DLTQLFSVTDVTCQAFLRHQSVEQLLEFLSQDLDLTVEPDVSVADYCFSPYPCGYFGY
                     CTRHLPEENIFMLKNVNKKNKLDMYNRGLYSFSDVINERVVFSRLRPVVKDQLLKEKI
                     IDKKEIGRFVSKFRYPLYYLDFETIQYAIPPYDGMKPYQQTPFQYSLHIEYEDGRLEH
                     KEYLASDGFDCREQLATRLVNDIPKGACSVAYNMAFEKGVIRDLADYFPYLRDELTDI
                     YDNMLDLMIPFKNHWYYDKEMQGSYSIKAVLPALFPDDYTLDYENLEGVKRGDQAMAA
                     FRKMPSMSEEEKQQVRNQLLEYCKLDTYAMVMIMRKLKGAIYE"
     gene            complement(31623..32186)
                     /locus_tag="PLF14_03445"
     CDS             complement(31623..32186)
                     /locus_tag="PLF14_03445"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOT70385.1"
                     /translation="MKILFIRHGQTDWNIQERVQGSSDIPLNETGIKQAHAVKEMLKK
                     HSFDEVIVSPLERARHTASIVCEGRDVEMIIDRRIAERDFGKYEGVSYRQDTEYYKKV
                     WSLDMNNQLETVESYIAFYGRVVDFLEEIIKKHYGKTVLLVAHGGVSIAVAQYFKGAP
                     ENGDYRSYLLDNCEIAEYDETYIKKKL"
     gene            complement(32214..33092)
                     /locus_tag="PLF14_03450"
     CDS             complement(32214..33092)
                     /locus_tag="PLF14_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70386.1"
                     /translation="MLNCVPSLIACIKTTTKIAIAYYDGYIYYVGIHQGNYWLFRMNM
                     DKNNHIPVKLISTMQERLTGFFEHGYLYFFKGSIALLGNTNNVIYKTAVFDNSESVKI
                     ADAEDKGIAVGNLSWFYPLDGYIYFYTYFPETKYRLCRYSMETGQWSEFVNTEWCYEN
                     VFTDNDKVYWYLNGKGFYEYTYETGITEKVADFIGDGLYVASYNDEYIYLYQFFETKE
                     SSVSPMFYIFDRQYNLIDSVLFDVEMEDYSLTFYVTELEDYVILSSAVISNPPDYYLL
                     KSEFGSGDIKVRKIGD"
     gene            complement(33022..33360)
                     /locus_tag="PLF14_03455"
     CDS             complement(33022..33360)
                     /locus_tag="PLF14_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70387.1"
                     /translation="MKKRLIALFLLFTLLIVLSSCKTFNEKNLDTDNITETSNVFNGD
                     INDNRFTRFRQSDSVISLFVETHKGIYYLNCKDCSVYFSFDGISEYVKLCSKPDCMHK
                     DHDKNCNCIL"
     gene            complement(33365..34126)
                     /locus_tag="PLF14_03460"
     CDS             complement(33365..34126)
                     /locus_tag="PLF14_03460"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOT70388.1"
                     /translation="MNQRQLQIKRMLDDHSEVFLKQLKKIFSDVSEMTIRRDLILLED
                     KGYAVRTHGGAVSINRFKKAGEDETGYTVRQAENVEKKAIIARKASHFIEKDRSVYLD
                     AGSTVMGLANLLTDMKGCTFITSAVNTALKLASTDNSVILLGGTISPNTLACSGPEAM
                     QMLEIMNIDLAVMGASGFSAEKGFSVSNMHDAKLKTRIIEKARKTIMLADSTKIDKDK
                     AYTFCRLKDVDIWVCDTMPDDKVLDKCKKDNVLVV"
     gene            complement(34133..36595)
                     /locus_tag="PLF14_03465"
     CDS             complement(34133..36595)
                     /locus_tag="PLF14_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOT70389.1"
                     /translation="MTKELVLNPKDLRKRSKIKFKDIDVCAYNKTIEEEKKIFSKADF
                     LRIFRDMAIIREFESMLNAVKTTGQYNSVEHAYPGPAHLSMGQEASVVGQAYTLGIDD
                     FILGSHRSHGEILAKGLSAIEKLSDKELMDIMESYFDGKILRIVQKDQKTVKELAIDF
                     LLYGTLAEIFSRETGFHRGLGGSMHAFFIPFGIYPNNAIVGGSADIAVGAALYKKVNK
                     KSGMVIANLGDASLGCGPVWEGICFATMDQYKTLWEGEHKGGLPIIFNFFNNFYGMGG
                     QTMGETMGFDILARFGAGVNPEQMHAERIDGYNPLAVIDAYKRKREIIEKKEGPVLLD
                     VMTYRFAGHSPSDASAYRTKEEIDMWMEQDSLIEYRQKLVKGGIAKEEEFEAILGDAA
                     SLIFKIYKMAIDPSITPKMDLVGNPKAIEDLMFSNERIEKMDDRPVDALIPLEENTRY
                     QQVQKKERRGIVDGVVVSKNKVFQIRDAIFEAMIEKFYTDPTMVAYGEENREWGGAYA
                     AYRGLCDSMPYHRLFNSSISEAAIVGSAVGYAMAGGRAVVELMYCDFLGRAGDEVFNQ
                     LPKWQAMSAGILKMPVVVRVSVGAKYGAQHSQDWTSLCAHIPGLKVVFPVTPYDAKGL
                     LNAALSGTDPVIFFESQRLYDKGEMFHEGGVPTEYYEVPIGEPDIKRAGKDITILTIG
                     ATLYTALDAADILKEKYGMEAEVIDARTVSPFNYDTVVQSVKKTGRIILASDACERGS
                     HLKDMATTISTLCFDYLDAPAVVVGSRNWITPAADYEKSFFPQPSWILDAIHQKIVPL
                     KGHVSTMSFTDGEIVRLNRLGV"
     gene            complement(36650..38017)
                     /gene="lpdA"
                     /locus_tag="PLF14_03470"
     CDS             complement(36650..38017)
                     /gene="lpdA"
                     /locus_tag="PLF14_03470"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOT70390.1"
                     /translation="MKYDLIVIGGGPAGYLGAERAAGKGLNVLLFEKRYLGGVCLNEG
                     CVPSKALLHSAKIYDYTKHSDQYGIKVTDSTLDQEAVIKRKNKVVRTLVAGVKGTLKR
                     NKVTVVEKVAKIEKKSSDGFVVSAEGELYTADRLLICSGSEPIMPPIPGLHEGYQKGF
                     VLTNREILDLTVIPETLVVVGGGVIGLEMASYFNSAGSKVTVVEMLNKIGGPIDDEIS
                     DLLRKEYEKKGIMFNLESKVTSFTDKSVIFEKEGKNVEIECEKVLISIGRRPSTKNLG
                     LENIGVETDKRGAVITDRFGCTNVAEVYAAGDVNGISMLAHTAYREAEVCVNNITGKK
                     DIMRYTAIPAVIYTNPEVAMVGETEASARQKNMDFEAVTISMKYSGRYVAENEGGNGI
                     CKALREKSTNRLIGVSLIGNYASELIYGAAMMIESQMTVEDLKELVFPHPTVCEIIRE
                     ALFAF"
     gene            complement(38031..39350)
                     /locus_tag="PLF14_03475"
     CDS             complement(38031..39350)
                     /locus_tag="PLF14_03475"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOT70391.1"
                     /translation="MATAVIMPRQGNTVESCVVSKWHKKKGDKVNIGDILFTYETDKA
                     TFDEEAKVEGILLETYFEEGDDVTCLLNMCVIGEPGEDVSSFAPGGEQPVEQSVEKPE
                     EEVKVEKESVRVQTTVTDQLKISPRARALAEKTHVDVSYATATGPDGRVIERDIQALV
                     KEGVKVTPAAKTGYDKAAQYDATGIGGRVTVEDLSKGKISVSADEISTADYTEEKLSN
                     IRKVIARTMYSSLSGMAQLTMNATFDATSIMNFRAMVKAYGERLGLANITLNDVIVYA
                     TAKTLLLHKDLNAHFNGETIKRFNVVNMGVATDTPRGLMVPTLFNCDKLSLNEISVAV
                     RDLAGKCRSGSINPDLLTGGTFTISNLGNLGVETFTPIINPPQTAILGVCGLTTRIKE
                     ENGQIKTYQAMGLSLTIDHRIVDGAPAARFLKDLCNNLENFETMGAK"
     gene            complement(39827..40153)
                     /locus_tag="PLF14_03480"
     CDS             complement(39827..40153)
                     /locus_tag="PLF14_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70392.1"
                     /translation="MLRPDPITTYGETRQIGLYNEKDFKEIPFSSESVFDVIADTIEY
                     NAEYSEKKQACFVTVKDKNNNGQIVIKISSDKVKPDFNIFYSEWLTCDIVNNGITEEP
                     WLCLFV"
     gene            complement(40288..40809)
                     /locus_tag="PLF14_03485"
     CDS             complement(40288..40809)
                     /locus_tag="PLF14_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70393.1"
                     /translation="MEIKTCLEKNAFENENIVLSYLKRKKEVPEVNIEDNRAFRLVED
                     ISKKGGNITVEIYEDIIVKKINYTDNLSLTVCEAKKEFVKNNSELIRITKNNKGMFIN
                     TKGSYILDYKDVNEDNVEDIILINGTNGKISKDNVYYLNGANLEKQDVYVCETMHKTP
                     DEYPMQLIHSIRG"
     gene            complement(40868..41263)
                     /locus_tag="PLF14_03490"
     CDS             complement(40868..41263)
                     /locus_tag="PLF14_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70394.1"
                     /translation="MRKRNLFLFTFIFITVMILTSCTKTHEETPKPVITVLPDETPNV
                     IDISDKTGFDFKIYYDGKDELANFTNLCFEYDGKKTEVIENIKLSEKGIPVVIKEDID
                     FDGKYELIIYLQEKTEMYYIQGKNMFFRL"
     gene            complement(42135..43487)
                     /locus_tag="PLF14_03495"
     CDS             complement(42135..43487)
                     /locus_tag="PLF14_03495"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HOT70395.1"
                     /translation="MKKSMAVLLILIVLSLTACNIGSKPAKECREGSFIYIVKDNEVT
                     IIKYDGTEETVIIPDRISNKPVACIKDKAFSACSHVKEVFIPESVKSVGVGAFDDTEW
                     YKSLKDEFVVAGDGVLIKCNQTSKDIVIPEGVKSLSSTFYKAGDGGFFDAVIYLPATL
                     KKIDDYAFYKAFGHIIVCNTEQIEEIGQSAFEDSTVDRIIISDKLKSIKDRAFATTDK
                     TVKRSMTIEMTGGGYYVGTQKKVKLPFATSLGKDIFYGLDGNMETVKFEDGTTAITKD
                     MLTCLQSFAKFFIPGEVTCIEKDIFPEGAIIYTEAGSAAEKYAKDNSVKVLILPESFK
                     DRALEKLLIPAFLSANLEFTYDNMQIITSIQIRDQVEGNVYDHYRVYVIVQAATGAKG
                     IEHILKQDNVKSLEDLKFFTKLEYLLLESAIIDGSVDCAVLKSMPALKEAVVNNESLK
                     "
     gene            complement(43509..43892)
                     /locus_tag="PLF14_03500"
     CDS             complement(43509..43892)
                     /locus_tag="PLF14_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70396.1"
                     /translation="MGQNLSETQYNDLVADEANGVHLSITHRSAIFKLNNAYEDIVLL
                     SVFSTNSDLSPITSGLSLYELRAMIKNIWGTQISHGISVDGGDSTQIVTKENNVRKCI
                     RTGDWQSNPGVYQPRNVYTMICAQV"
     gene            complement(44234..44878)
                     /locus_tag="PLF14_03505"
     CDS             complement(44234..44878)
                     /locus_tag="PLF14_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOT70397.1"
                     /translation="MKSVLIIGTGKFGKCLALKMAELGNEVMVVDKDENELKEISEHV
                     TSALVADCMDENVLKSFGINNFDICFVCIGENFQSSLEITSLLKDNGAKYVVSKAERD
                     IHAKFLLRNGADEIIFPEKDMANRAAYRFSANHMFDYIQLSPDYAVSEIKTPEQWIGK
                     TISHVNVRMNFNVNVLAYKEDGKIHPLSNPNYIFKEHTRLIVSGAWKDVEKISK"
     gene            complement(44886..46229)
                     /locus_tag="PLF14_03510"
     CDS             complement(44886..46229)
                     /locus_tag="PLF14_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOT70398.1"
                     /translation="MEAKRKIRLNTMQIIAIGFVVIILIGALLLSLPAASKDNTRIPF
                     INALFTATSALCVTGLATYDTYTQFTLFGQIVILVLIQTGGLGFMSVAGMIFLMLGKK
                     IGLRERGLLMETMNTPHIGGVVKLLRRIIITSFAIEMIAAALLSIRFIPLLGTVKGIY
                     YGIFHAISAFCNAGFDLMGVFDSRSSLIGFEADVLVNVVAILLMLIGGLGFIVWDDIM
                     DNKLKFSKYRLHTKIVLIGTAVLVVFPAIFFFFLESNNTLAGMTFGEKVLASVFQVVS
                     PRTAGFNTVEISELSEGSLLLTILLMIIGANPGSTGGGIKITTVFVIILTIKAYITHE
                     PDLNVGGKRLEDFIPRKAFSIASIYVILASLACFFIVSIQPFTLEDTMFETVSAISTV
                     GLTTGITPELNILSKIAVIILMYCGRVGSLTVIAALAGDKPRPNIRQMEERIIIG"
     gene            complement(46233..47297)
                     /locus_tag="PLF14_03515"
     CDS             complement(46233..47297)
                     /locus_tag="PLF14_03515"
                     /inference="COORDINATES: protein motif:HMM:NF025957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOT70399.1"
                     /translation="MQKAKTYYKGLKPYTNLDDKDLIYLDIRNKPFDIYGLYDPLNQP
                     VFKRMPDEVAAKSNSRVRIHYLHTSGGRIRFCTDSDYLCIQVFFPELFRMTNMSLQAA
                     SGFDIYEDFDNRSVFFSRFNPEVNTKDWYEMRIRFSEHKLRYFTLYFPLYNNVDKVYI
                     GVSENSLLGPGKQYKHKKPVVYYGSSITQGGCASRAGNCYEAVISRMCDVDYINLGFS
                     GAAEGEQAIAEYIASLDMSVFVLDYDHNAPKIEHLQKTHQPFFNTVRAKNPDLPVIFV
                     SKPDFSDYSFDSIQRRNIIFKTYMSAMENGDRNVYFIDGRSFFARDYRDSCTVDGCHP
                     NDIGFLRMAQVIGNMVKELL"
     gene            complement(47320..49344)
                     /locus_tag="PLF14_03520"
     CDS             complement(47320..49344)
                     /locus_tag="PLF14_03520"
                     /inference="COORDINATES: protein motif:HMM:NF017215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HOT70400.1"
                     /translation="MALGIGIDTGGTYTDAVIYDFDSGKVLCSAKALTTHEKLETGII
                     NALSRLDKQYFSKVDIVALSTTLATNACIENKGGRAKLLFFGVEQKVFDMVGKDYGLT
                     DKSDVYLCEKSGDFSGNNTQQPNWDKLFTQLDDWLNDAEGLGIVEVFAASNGAKLEKT
                     AKKLVEERYGIPAVCGHELFYELNSIQRGAGTLLNAKLIPIIKQFLESIKSALEKMHI
                     NAPVVVIRSDSTKMSEQYAETKPVETILSGPASSVTGGVNITEEKNCLIIDMGGTTTD
                     VSIVKNGIPLNADSGVRIGSWKTFVHGVFIDTFGLGGDSTIRVEHGKVVQLPQRMIPL
                     CVAATRWPGIIEQLKELIDSKKYHSYPLYEFLLLQKSVTGNNSHIIHNKYTKEESELI
                     NLLEKQPLPLEKAANALGKDIYTFNTERLESEGVIIRAGLTPTDIMHVKKDFNAFDET
                     ASITAVKYILSNIFEETGIEYTQEEFCDMVYDTVKLKLYENIVRILLTDKYPDDFKSG
                     VNEQTMALIRNSYKDRLHKDLPVRFATDMSLVGIGAPTHIFLPDVAKALGTGCVIPSD
                     AKYANAIGAVVSNVRSVYQIEIRSEYDSSYMVYAPSGRYVTNDFDEAVEYAKKAASEA
                     ALKDAQSKGIADNYNINTQVITNTATAKKGEQINLGTTVIATVSGNMQYN"
     gene            complement(49335..50591)
                     /locus_tag="PLF14_03525"
     CDS             complement(49335..50591)
                     /locus_tag="PLF14_03525"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT70401.1"
                     /translation="MITIAVIGLGNRGSGYMRWTKLLNGRKAKITALCDTDEYRLNTY
                     GDKYEVDKSNRYLNEVDFFKAGKLADAIYICTMDNDHYRHAMAAIECGYHIMLEKPVS
                     INIAECEEIRNKAKEKGVYVVVCHVMRYSPFFKKMNQLVKSGKYGNIISIEHTENIGY
                     FHFAHSYVRGNWRDSEKTTPMLMAKCCHDFDLIHWLMGDGCKSISSYGSLSYFKKENA
                     PEGCGDICLDCPKHIKNNCPYDAESLYITAPIWRFTFLKLLGDVITGKPKYNRKDKYD
                     SLRTGLFGRCVYKCDNNALDHQVVTMDYGDNRYVNLNVTAFSEKCYRHTILHMEKADI
                     HCDESKGRFIINRFGKGSKTVYAGVIRLAHISGDMALVNNFIKLLSGEPVETDDLTFI
                     DTTIVSHRNVMKAEQSRKEGGKVIWL"
     gene            complement(50588..50938)
                     /locus_tag="PLF14_03530"
     CDS             complement(50588..50938)
                     /locus_tag="PLF14_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HOT70402.1"
                     /translation="MNIQIFGKTKCFDTQKAQRYFKERKIPFQYINMQDKGMSKGELE
                     KVLRAVGGMENLLDYKTKDQDKLLRIKYAKEQDRTTLLFENQHLIKTPVVRNGNKATA
                     GYEPGVWKEWEGDK"
     gene            51074..51697
                     /locus_tag="PLF14_03535"
     CDS             51074..51697
                     /locus_tag="PLF14_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70403.1"
                     /translation="MKAFFVKYRRLILLMLLFLLYIFYLIVCAIVKKSDNFFSTEMFL
                     DSYIPFIDFAVYGYISWVPLAAVSVIFLFFCPGDGYYRLIIAIAISVLICLIISLSYP
                     VRMDIPHFGDSAFLVAVLKNSGIASFPNIPSCILSNGFFLMIFYKKIHKRSKCLPIVL
                     LFGLILLAWIVCALLSKMTHISDLLIGILIGAVSSLSVWFIPFKQTH"
     gene            complement(51694..52527)
                     /locus_tag="PLF14_03540"
     CDS             complement(51694..52527)
                     /locus_tag="PLF14_03540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HOT70404.1"
                     /translation="MNKFNTIHLHTPYSDGINYSHEYIEEAIRLGLKSIGFSDHAPVP
                     LKYDWAMKKENLPRYFDELNYMRKVYGNKINIYIGLELDYLEGLDVKKYIDFDQLNLD
                     FVIGTVHYLYSDRIDGFASADGTQERVDKAIIEGFGGDGVAFYKKYYQTLRQMITEYN
                     PLMIAHFDIVEKRNIGKKWFDADTDEYKAHVEATLDVIKDSYIEVNTGAMAKGIDVMY
                     PSEYILRRCRAKNLRVSVNSDCHFTSALLFAYDKAIDRLKRAGYDCSYVFDGVMKKVK
                     F"
     gene            complement(52520..53455)
                     /locus_tag="PLF14_03545"
     CDS             complement(52520..53455)
                     /locus_tag="PLF14_03545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013082946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT70405.1"
                     /translation="MKKLYSLVIPLYNEEQVIHECIKRVTDVMKDKDFDYEVIFVNDG
                     SKDATETIVKQYCAEDAHLKLISFSRNFGHQTAITAGMDHSAGDAVIVMDADLQDPPE
                     VVLQMIEKYEEGYDVVYAVRSKRKGETFFKKFTAKVFYRFLKSMCEVDIPVDTGDFRL
                     ISRQVCDVLKGLTERNRYVRGLVSWVGFKQTGIYYEREERFAGETKYPLKKMLKLSMD
                     GITSFSTKPLKLTKWIGLVTTASGFIYLLVVIIQRLMGIRLQNGWIPAVIAILIVGGI
                     QLIMSGINGEYIARIFDESKNRPLYIIREKVNMDE"
     gene            53678..54058
                     /locus_tag="PLF14_03550"
     CDS             53678..54058
                     /locus_tag="PLF14_03550"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT70406.1"
                     /translation="MDSLYKNFGSRVREKRVMNNMSQEKLAERCDLSTSYIGLVERGE
                     RKASLEVVVKIANALDVTPNALAMDSIKHKACNNKDKLVLMINDFDDAQLNTTFEIVN
                     QIHSYIRKGTKKATYMDIDELLSD"
     gene            complement(54055..54249)
                     /locus_tag="PLF14_03555"
     CDS             complement(54055..54249)
                     /locus_tag="PLF14_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008590330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HOT70407.1"
                     /translation="MKTDIQLNDIVELKKAHPCGSNKWRILRTGADFRLECLGCGHKI
                     MLTRNDLFKKVKNVLKEDKD"
     gene            complement(54249..54884)
                     /locus_tag="PLF14_03560"
     CDS             complement(54249..54884)
                     /locus_tag="PLF14_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70408.1"
                     /translation="MKKVIIYILIIAILSTMLVGCNKNIEIVKPEHVSRGEVVDGYYV
                     NEDMGFKIKVPENYYTATEEELDTKFDIKQHVEDDELEVFITYLAFFSQKPVDDAQDN
                     SHIMISVEDASKYGYSDKDTYIEYMSNSKADTYRNIEGAKITLSNQTNVWLGNRKFAN
                     RTLTLDLHDLYMIFDMYAVVKNGYFLSIMIGAHNEADLEYLRGYLDTFTFE"
     gene            complement(54886..55302)
                     /locus_tag="PLF14_03565"
     CDS             complement(54886..55302)
                     /locus_tag="PLF14_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013403847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOT70409.1"
                     /translation="MRSYRKELYFNIGTRRGFVNITPQLEQSLKESGIREGLLLCNAM
                     HITASVFVNDDERGLHQDFEKWLEKLAPEKPYSQYAHNGFEDNADAHLKRTIMGREAV
                     IAVTDGKLDLGPWEQVFYGEFDGKRPKRVLVKIIGE"
     gene            complement(55387..56571)
                     /locus_tag="PLF14_03570"
     CDS             complement(55387..56571)
                     /locus_tag="PLF14_03570"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HOT70410.1"
                     /translation="MMSRLFYVSDFLKEGISDSQAIKDCLNSAKSCDERTIIFDKKDY
                     YIDAAITLPSDTTVIIDGVMIKQNDFVHDNVFRADNMETDPDNPYGYPLSIKAAKNIK
                     IIGKNDALIEGPKKHTSFWHPHFNEEQLAIGDYWGWRGFQVLISRCENFEIRDITFKK
                     VKSWTISADRSKNLHFSDLRFYSTVKNGDGLNIRAGCSNVVIENISGETTDDLIAINS
                     ATDVNLVYPFKQYVFPLIPSNICKDEESIENRYITDVSVRNVYTSGVSGVALLSRHGN
                     KIKNVSIDNVIDTHTGPFIHPLYPNATNLKLVYSYTGYFGETYTPGDISGVRISNVHS
                     KFFKTTVFFGDVVHDVCIENVTQQRSDGELITLHEQQDPKEIKVFCKNGTNNSCFTAK
                     SI"
     gene            56757..57557
                     /locus_tag="PLF14_03575"
     CDS             56757..57557
                     /locus_tag="PLF14_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003003498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOT70411.1"
                     /translation="MAKYKAIPDGYMTIGKAAKKMGVTVRTLQYYDKEGLLSPSGESE
                     GGRRLYTDKDLVVLHQIVSLKSLGFSLNDIKQRLISLDTPADVANALTEQADKIRSEI
                     ENLTASLNAIEQLKTEVLQMQKVDFKKYADIIVNLQMNNKYYFLIKKFDDDTLDHIRD
                     RFDRESSMDFINRFDALSDEILKLQKENVPQESEQCQQLTKAYWDLIMEFTNGDMSML
                     QKLPEIANFENTNTDWAKKQAVVNSYLEPAFVIYFKKLGINNVREEQQ"
     gene            57554..58369
                     /locus_tag="PLF14_03580"
     CDS             57554..58369
                     /locus_tag="PLF14_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003003701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT70412.1"
                     /translation="MNCAIQVSNLRKSYGSNTVLKGINLHIKEGEIFALLGVNGAGKT
                     TALECIEGLKKYESGEISVNGEMGIQLQSSSLPAQIKSMEAVKLFSKWKKANIDHEML
                     TALGIEEIKKKKYIELSTGQKRRLHLALALIGNPDIIFLDEPTAGLDVEGRLSLHEQV
                     RKLKSHGKTIVLASHDMAEVEALCDRIAILHNGNIVFCGTAAELTEKLGRKYLVHIKT
                     QQGDQTFATDNIEDTLLSLLGELKQKGLYLSDIKVDRGTLEQHFIEMTRGEEE"
     gene            58366..59082
                     /locus_tag="PLF14_03585"
     CDS             58366..59082
                     /locus_tag="PLF14_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004802043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT70413.1"
                     /translation="MKGFLYGLTLQCKLDLRSKTILVTCYIVPLLFFLLMGSIFGSVM
                     PEMKETLIPSMIVMSVSMGAFIGLPPSLIETYGTDVKKVYKANGIPLHVGLITMFLST
                     FFHLMISCVIILLLAPVLFDAASPKNIPLFLLALAIYAIVSLSIGSILGLVVKSQAKV
                     TMIAQLLFLPSIMLSGIMLPIEILPESLKILGRIFPATWGYRLMLDNGFHIKNLWYLI
                     IVFCIAVIVCMILLKKQKFK"
     gene            complement(59102..59266)
                     /locus_tag="PLF14_03590"
     CDS             complement(59102..59266)
                     /locus_tag="PLF14_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70414.1"
                     /translation="MKKVELKQDFSKMSKEEIDNLLNEGFLEYQRGETVPVQYAFEEI
                     RKKYGIERDK"
BASE COUNT        15958 a        13351 c        10424 g        19640 t
ORIGIN      
        1 atgaatcatc aatattcgtt atttctctgt tgacttttca tagttggtct cctttttctt
       61 tgttcttata gtattataca attaaattgc ggataattaa aatctgtttg aatatctttc
      121 caatgccttg attgtatact ttgagattaa gccggtaagc actcccgcta tcactgcaaa
      181 tatcgtaagg acggcaatat accagaatat gttcagattc tttaagatga ccaatgcggt
      241 aaaaaactgt gcagcattat gtattactgc tcctatcaca ctcaaaccta ttacggaaaa
      301 ctccaccctc ttctgccttt taatcaatga gcatattgtt atggaagcca ttcccccgaa
      361 gaaactgaac agaaaggtaa caatatttcc ccgaagcaat cccgcaaccg atactcttac
      421 ggcctgtacc gatatggcat atgcgggtcc gaacacataa agaagcgtca aaggtaccac
      481 attggccaaa cctaatttta cgcccggagc tattgcggaa aaaggtataa agctttcagc
      541 aacactcaag gctattgcca aagctgcgaa tattgcggat atcgttatct tctttgttct
      601 gttcatcttg tcacaccgtc cacttccgaa tccgttgata taaggtaaac aagaacttta
      661 ttgggaagac aggctatctg tttttttgag cttggtgtaa gtttccccat atgcttacaa
      721 tccttgtttg cacaatcggc aaaagacata tatgcggtac cgtctttaat agttacgata
      781 ttagttatat taccgtatcg gtcttttatt tgtatctcat tgtctttgtg tatggactct
      841 ctgtaaataa cctttgaatc gtgctgtatt acaagaatat cgcttttttt tgaatcattg
      901 aaaagatgca gtcccaagaa tattaagaat acgcatataa gagtgactat gagaatgatg
      961 tccgcctttt tcatatgcgt attttatcat taaccgaacc tgtttgacag ttctttatac
     1021 atttcttcgt ccttttttga cattcttaca ggattgacta tatttacttt gatatataag
     1081 tcgcctcggg tcttatcctt tttatagtag cctttttcct ttatccttat cctggctccg
     1141 cctcttatct tttcgggtat ggtaaccttc agggtattgt ccggcatttc cacctggatt
     1201 accgaaccga aatatgcctg ccagggatat atatccaccg tttttataag gtcatcattt
     1261 tccagtgtca ggttgggtgt gtcctgaagc tctattttta tataaaggtc tcctcccccc
     1321 tgtccctgtc ctttcatctt tatcttctgt cccgaccgta ttcctttggg tatctttaaa
     1381 ttaacctttt tatcattaat ggaaacaatc atttcccttt gcgccaacgc gtcctcaata
     1441 gaaactttca tggaggcttc actgtcaaac tgacgggatg aggctttgga cttgcgtccc
     1501 gaagactgcc cgaatacatc ggctcccata cccaagtccc cgaaaaacat attaaagaaa
     1561 tcggaaaaat cattggaccg tgagaaaccc gaactgccgc cgtaattata tgagtattgt
     1621 tgtccgaatg ccgacggatc gaaacctgcg gaagaaaagt cattcatgcc catggtgtca
     1681 tagcgctttc tcttttcatc atcgtaaagc acctcatacg cctcatttat ttccttgaac
     1741 ctctcctcgg ctcttttgtt gccgggattg ttgtccggat gaaatttctt cgccagtttc
     1801 ctgtatgctt tttttattgt atccttgtcg tctttcttat ctactccgag gattttataa
     1861 taatctttat actccatatc tttcctttca gtctgacttt ctctgaccta tgggtatatt
     1921 atatatctat tatacaatct tttcaactgt attctatctg ttcaataccg caactaaacg
     1981 acatgaggta aagtatgatt ttttgaacgg aagaaaatac catatactcc atatggtatt
     2041 ttatttatat aaatatgagg attttacaga ctcgcctgta tcactaatat gatacgctga
     2101 agaatcataa cgataatgta taaagcaatg aaagaaaaat caaacatcaa aggttttcct
     2161 ttggtcaacc atgatatgaa atttcttaaa ggtgccagga aaggttcggt catccgtaca
     2221 agcacttcat agtatttgct gaatcgtgca ccgccgaaat atgatgctat cacccttata
     2281 aacacaagaa atgacagagc atatagcaga taatatatga cggtcagtac ggtcttgagc
     2341 atcttacctc tttagttggc atataaccgg gatttggtgt cggttacttt agccggttcc
     2401 tgcttttcat catcgatatg atagccgcta ggagctacga ctaatatctt gtctgaaagt
     2461 tttcttatag cgccgttaag ggtatatacc gtgccgctca aaaagtccac tattctctgt
     2521 gcaagatcct tttcaacctg ttcaagattg actattaccg ctctgtttgc tttaagcgaa
     2581 tcgcagactc tctttgtctc ttcgaaactg cccgggtaca ttacattcaa agtacctgtg
     2641 caacgttcca tgggcactac ttttttcgtg gcaatctgat ccaatgcgtc aattctctcc
     2701 acaataggat taatctgaac agtagatctg gttttaccat ctcttgttac cggcgctata
     2761 gtttgttttt cttctgctgc ctgttcgaaa cctaagaatt cggttagaaa cttgtcaaaa
     2821 acactcatgt tttcctcctc ttttgtgtac aatcaaatag tctttagtat tttatggaat
     2881 atccttaaaa attgctgaac cgatgcgtaa tatattggcg ccctccttag cggccaacat
     2941 ataatcgtta gtcatcccca ttgaaagata taccatatgt gtattgtaaa aggtattttt
     3001 gcttatgtca acataaatat catgtaattt tccgaatatt tgagatatta actgctcatc
     3061 atcggtcaaa ggagccattg tcattagacc ttttatcaaa acattggggt tattttcatt
     3121 gtgatttatt aaatccataa ctgccgactc atccacaccg aacttggttt cctcgtcaaa
     3181 aatcttaacc tgtatcagta catttgtaac aacattgctc attgccgacc gcttctgaat
     3241 ctcatcaagc agttctttac tgtccacaga atgtatcaga ggagttttcc ccacaacata
     3301 ttttactttg tttctctgaa ggtgacctat caggtgtatt tttgctttac cctgaaaata
     3361 ttcatacttt ttcaagaact cctgcactct gttttctccc agctccacac ccggaacttc
     3421 cagcgccaga gccgcagtct cagaggtaac ggtcttggac accgccacta tcagaatgtc
     3481 gtcattgttc agctgtttct ttattctgta tatgttttct gcaacatagt tatgcgaagt
     3541 cattttctca ccctcagtta attatatcac cgtccgacac tttatccggc tgcagtatat
     3601 aatagtcata tgccttaaaa ctgtaatcat catcggcatc ctgtactatg gcatatatac
     3661 cgtccgagta taaaacatca atctttacaa atttcaccga tatgcctttt accagtgcaa
     3721 tctctgtctg ttggaaagag gagtatttat aattctttat tgcacttacc ggcactttaa
     3781 gaccggaata ctctttgata accagcaaac cgtcgaatct gcccaatgaa acgagagaag
     3841 aaagcatatt gtcggtctcg aaaaatacta ccgtctcccc atcggaactg tttcccgtta
     3901 tcactttcgt cacttgcact tcaaaatcca gcccgccggt atttatgcgt attttcaatc
     3961 cttttttgtc ctttatcctt gcggcatcgg aagaggaaag tattgtcgct atgtggaatt
     4021 ttcttgaagt ggcaactttt atcacggtgt tacctgtata atcccgctca catgatatac
     4081 tgccttttat atcttcggga tacattccgt acatggcatc atatgtatga ttgtgctgtc
     4141 cgttgtacat attgtaagtc acatatccgg aattgacaca agctatgtcg gtagcatcgc
     4201 ttgccattgc ctgtcgtaac tctttctgcc tgcttttaag ggcatttatc tgcaactgtc
     4261 ttgtattgtt tccgctcata aggtcggttt tgtattcata caaagagtca atattattct
     4321 tcctctccac tgcatcagcc attgcgtttt cattcagaag agccgatagc gacagaaaat
     4381 tgtctttaat ctgtatatct ataagttcga tatcctttga aagaataccg gtattaatct
     4441 gatacagttg ttgaatatcc gtaatacggg cattgatatc gcataactct tcgtataaag
     4501 gatttttcac attagcaagc acccctattt tatggttaac acccgctctg ctgccttctt
     4561 cggctatttt tattaaagaa tgatcggaga cagtatcata tgaatcgaag tcatataaga
     4621 ccagtccgga gatatttatc tgatccgtaa gtcttccttc gtaagccaga tcttttttaa
     4681 caatagatga gtaatagcgg attgccatac ggcttatcaa tattgcaaac agcacaaaag
     4741 ctatgattat caatatctta ataaccgcac ccgtgttctt gcctgattgc tttttttccc
     4801 gttctttctt catcggatat caatcatacc ctttttaatc ataatatcac atgccttaag
     4861 tacacccagt ttcacctgag tataggtaag acctccctgc atatatgcta catagggatc
     4921 tctcataggt gcatcggcgg aaaactctat ggaagcgccc tgcacgaatg ttcccgccgc
     4981 cattacaacc ttgtcgtcat atcccggcat ttcccacggg cagggggtta catgcgaatc
     5041 tatcggtgaa ccggcctgta ctccccggca aaactgtaaa agctgttcgc ttttgtcaaa
     5101 cagtaccagc tgtattatat cttccctcaa ttcatcatat ttcggataac ttttaaatcc
     5161 cagtttttca aatagcaatg ccgtgaagac ggcacctttc agactctcct taacggtata
     5221 aggagccata tacagacctt gaaacataag ccttttattg cttgtcgctc ctacttcctt
     5281 taacagaccg ggagcataca gccttgcaga tgcgttatct atacactctt ttgtaccgac
     5341 tatatatccc ccgcttttac acaatccccc tccgggattt tttatgagcg accctgccgc
     5401 catgtccatt ccccattggg taggctcctg ctccttaaca aactctccgt agcagttatc
     5461 aacaaacata ataatatttt tattatatcc tttaaccact gtgctgagtg tttcaaactg
     5521 ctctttatct acgggaggtt tgatgctgta acctctggat cgttgcacaa ataccatttt
     5581 tgtctttttg ctcaagcttg cagccacggc ggaataatca attttattat ccttcgtatc
     5641 aattatctta tgggtaacac cgtattccgc aagacttcct ctggcttcgg ttatacccac
     5701 tgtacttctt aaagtatcat acggcaaccc gttggcaaaa attatttcat cgcccggacg
     5761 aacattggac gccattgcaa gcgatatggc ggcggtaccc gaagcaatgc tttcccgtac
     5821 aagggcatct tcggtacgga atatatcggc atatacttca tccagaagtt ccctgcctct
     5881 gtcggaatag ccgtaacctg ttgtttcggc aaaatatgca tcacttaccc tgtttctctg
     5941 aaaagcagcc gttaccttaa cctgatttgt ttcggcggta tcctccagtc tcttaaactg
     6001 tgcatcgcat tgttgctgta cgatatcggc cagcttgata agtctgttat ctatgtcgaa
     6061 attttcaatc atcgtaatat ccctttgatg cattttatag cgtcggttat tttaataaca
     6121 gcctgccccg ataagggagt aaactgatag gaaaactgtt cctgatttat ctcaaaccgt
     6181 aaactgtagt cgcacacggc atcggtaatg ctgtggaaag gcaacacggt ttttccttcg
     6241 ggatatgtgt agataagttc aaccttagta attttaagat aactgtccgt atactcttta
     6301 agtacctttc cgtagctgtc ctttacggta agcgaaccgg gaaattccat tacaaaatca
     6361 tcggaagcta cttccttttc caggtgcgtg ttttgccatg ctctccattc gggcatatca
     6421 ccaaatgata cgtcatcttc cgtttgtgca ttcagcagtc cgtatttatc aaccaccgcg
     6481 gtgttatcgc attcatcgca gtacaaaaac atacctttac tcttcatgga ataatcggta
     6541 agacatttag ggcatttatg taatatggca tgcattcctt cggccatgga acggctttta
     6601 aattccacct ttttttcccg ctgccaatca tattcattgt aataaagagc ttctcttatt
     6661 cttaaatcaa tctgttctac cgacatggtg ctcacctgtt cttttgtcag cagcacatat
     6721 gccttatgtt ctattccgcc tcttctgaaa cctcccgaac atcccctgta ccatctcggc
     6781 aacgaaatgg aagctccgca aagatatgaa accaccacat tggaaccggt tgtttttatg
     6841 agtttggcgg cactgttatt aaacggaagc atggaaccgt caattgttct tgctccttcg
     6901 gggaataatc ctatcgccct gttttcttta agtatattta aagctatctt cgtggaccgt
     6961 atatcgctca taccctgacg cttgggtatg gctcccagat atgtaagtat ctttttcaat
     7021 gcgggatatt cgaaatatga ttgcgaagta agataatgca cccatctttt tgttacatac
     7081 gcacccatta caaaagggtc tattgcggat aaatggttac caataagtat ggtagctcct
     7141 tcaagtgaat caatatcatc ggatttctct gaacgaagat tcaataccgc ccggaatccg
     7201 gcgcgaacaa tacctctgac tatcgcttta aatactcttc ccggttcatg tatgtaatag
     7261 gtgtctgttt ttttcaatct tactccctca tcttttgcaa agtgtattct atcatttacc
     7321 cggcatgtta tcaatatatc ggccttcagg caataaaggt ttttagtttc acaggatttt
     7381 ttgtaatcac aacaaaacct ccatataacg aagaactttc ggcttcacgc gaagctgttt
     7441 agcatattgc atcagtttat gtatattcct gtctttagat gtggcatatc ttttcattgc
     7501 ttcacctacg atctgaatat cgctgccact accacgcaga atgtcgcaca atgtccgttc
     7561 caaatcgtat actcgaatcg gattacctga caaagactca atctcaatta ttcccgattg
     7621 atagttttta ggtataacac gctttactat tatgttctct ttatgcaagg acggagcgtt
     7681 ataccccttt ggaaaagtca tagtatattt tgcaggtgtg cggtccgaat agccaagcag
     7741 atacaaagct gtatcatgcg aataaatacc gcgtccgtat ttgcattgca gaagatataa
     7801 atcatcttcc catgcattgc tttttacata aataccacgt ccgtatcggt atatttctcc
     7861 gctctctaca agattctgca atacactacg atgcagtcct gcttccgaga cttgggatgc
     7921 ggtaattatt ccgttttcca atgtattcag cagtttatca atcttctcct ttttgcccat
     7981 actaatcacc tcactatgac aacaacacat taaattatag caatataatg tgtgattgta
     8041 cactgctccg gtcacgggtg tttagattat atcatattag atttctatgt attgcaactt
     8101 gctgcaactt aacttttata ttttttcttc cacaaaactc ctgcctttcg ggattttcag
     8161 cattattttg taattataaa ggacatttta acaacatatc gtatcttacc gtatcatatt
     8221 gcaacttact gcaacttaat ttaagcccaa aaaggtatat atttcatata tcttggagct
     8281 gtactttcat caaccgcctt aataagatta gcttctaaag tatcttttat gattctcgat
     8341 gccttgtatt tatctttatc gctgatatag aaaattgcga ccggttcaat acactaaagc
     8401 attctaaaag taatccttat gctagcaatg ttttctgcgg ttcttagcag taacgttgct
     8461 atccaaataa gcataaatta ttaacacttt tgttgaaatg cgtcactatt tggctaataa
     8521 tgcaggaatt tttgaaaact ttaatctcaa ttaccctatc tatagccgat atgttataat
     8581 tcagaaaagc ctctttaaag gcggtaagag tatcgttatt aagtaaaacg gattggagat
     8641 tacatatcat gcgctatacc ttatgcttgt tcgatcttga cggcacattg acagatccga
     8701 agattggaat aactaaatca tttcaatatg cactttcgta tttcggtatt catgaagagc
     8761 ttgataacct tacaaatttt atcggtcctc cgttgcggga atccttcaaa aacagttacg
     8821 gattttccga taccgataca gaaaaagccg tagcaaaata tcgggaatat tttacggcaa
     8881 caggtctcta tgaaaatgcc gtctacccta aaatccctga aacattggaa aagttaaaga
     8941 ataacgggtg tatacttgcc gttgctacga gcaaagttac cgtttatgca aagaagatat
     9001 taaagcactt caatcttgac ggatattttg ctttggtcag cggtgatgaa atggacggta
     9061 gtttaacaaa aaacggaaaa catgctctta ttaatattgc tttggacaga cttgaccctg
     9121 aacgcacttt gtcggttgtt atgattggcg accgtaaaga cgatataatc ggtgcaaaca
     9181 atgcgggaat cgacagtatc ggcgtattat ggggatatgg cacagttgag gaacttgaaa
     9241 cggccaatgc tacaaagatt gtccataccc cggacgaatt gtactctctg attattgaag
     9301 aataccggta aatgcaaaat tctcagcagt aaaaccaaac gacccgtact gtcatgtatg
     9361 actttgtaag tttctttacc ttgctatacc tctttaatta aaatacaatt ataccgatga
     9421 ataaaaataa gcatgacata tatatagagt tggaagtaca gccttactct cacggaagaa
     9481 gactttcttc ttttttgcga aaagaaatgg gaatatcaag taattgtata cgccgtgcca
     9541 aaacatttaa caacatattt gttgacggac aaagcgagca taccgactac attctttctt
     9601 ccggtcagac agtgggaata accgttcccg ataaaagcac taaatccgta atacttcccg
     9661 cagatatgaa tctcaatata ctctatgatg acgattatct tatagcggtg gataagccgt
     9721 acggagtact gtgccatcct attatggata ttgatgagga taccgttgca aacggattaa
     9781 aacatatata tccgaatatg ggcattcatc ctgtaagcag actggacaga gatactacag
     9841 gggtggtgct ttttgcaaag aattccgtcg tgcatcataa aatgtcaatc acagatattg
     9901 ataaaaggta tataggtatg gttataggga ttccgccttt agatgaaaca actatagata
     9961 tgcccattgc aagggtgccc gacacaccga tggagcgaca tttcagtccg acaggaaagc
    10021 aatcggtaac ccattatgag gtattgtata aagggtataa tgcaagcata ttatcttttc
    10081 gcttagaaac cggaagaacc caccagatac gggtgcattg taaagaaagc ggttttccca
    10141 taattcatga taccttatac ggttatcagt gcgaatacga cagatatata tccagacagg
    10201 ctttgcactc ttgctcggta tcgtttattc atcctttcac aaatgaatat attacaataa
    10261 cttctcccct tcccgaagat atggcaggtt tggaaaaggc aataaaggag aaatcacttg
    10321 ttaatgatta atgtaggtga tatgctttga aataatggag tatttaaaac taaaaggcat
    10381 tgtttgcaag ataaccgaat acaaagaggc ggataaaata ttaacccttc ttacctatga
    10441 aaaaggaaag gtgcaggtta tagccaaagg agtcaagaaa aaaggtgcgg cactgtcaca
    10501 tgcggcaagg cttttttatt gcggcgagtt tgaatgtgta tgcaagaaca atatgcctat
    10561 actcaccgga gcggcaatga tacaggactt cttcggtctt acaaacaaca ttgaaacact
    10621 gtattacgct tcacactttc tggatgtctg ttcctgtttc atacaggcgg aacagccttc
    10681 gggggatata atgtcgcttc ttctgaacag cctttatctg ttgatgaaga gcaacagcga
    10741 ccctttgctt ttaacctgta tactgcactt tcgcatgtcg gcaattgaag gtgctgcacc
    10801 tgttaccgat ttctgtgtgg aatgtaacaa accgttagat gacaattgca ctttgggctt
    10861 ttctttggaa caggacggtg tgacatgttg caaaaacgga aagggtatat caggtgccgt
    10921 ttatcaggca ataaatcaca tctgcacggc cgattcgtca aagatattct ttttaagcat
    10981 acctaaaacc gatttgcccg ttctgtatga actatcctgt aagtatatag agaaaatagc
    11041 cggaaggcat tttgaaatat tggacagaat tgaggaacta taggtttact tcttttctaa
    11101 aatttatatc ctaatttatt taacatcctg ctgtcgtttc gccagtcact ctttacttta
    11161 atccacagtt ttaaatttac tttgcagtca aaggaatact ccatggattt tctggcttgc
    11221 gttgctatct tcttcagcat agccccgcct tttcctatta ttatcccctt atgagtttct
    11281 ttttcacagt aaatgattgc atcgatatca tacagctgca tatccggtct ttgccggaag
    11341 ttttccactt ccacggccac accgtgaggt acctcttccg acagaaaata cagcattttt
    11401 tctcttatca tttcttccgc catttctctc atggtctgat cggttatctg atccttgtca
    11461 taatacatag gtccttcggg aaggttgtct attatgaggg ttttgagaga cttgatatta
    11521 aatccgtctt ttgcacttat tgctataact tcatcaaaag cggctttttc ggtataaaga
    11581 gcaataatac tgacggcagt ttgttggtca atagtatcgc atttgttcag aacaaatatt
    11641 ttaggtatat tcttcacttc ggacaaaagt tgtaatatgt gaacatcctc cttaggtatc
    11701 ttttcgtcct tttgcaaagc ggtaatgaaa ataatcacat cggtttcttc aaatgccgat
    11761 ttggtagcat taaccatata ctctcccaat cgcgtcttgg gctcatgaat tcccggagta
    11821 tcgcaaaaaa ccatctgaca gaaatcgtcg gtatatatgg ctttgatatt ttttcttgtt
    11881 gtctggggtt tgggtgtaac aatgcttatc ttttcttcgg taagagcatt tatgagggtg
    11941 gatttaccta cattaggtct tcctataacg gtaacgaatc ctgatttgaa acccatacta
    12001 ctcctctttt ttccacgcct taatgtcaaa agcattgggt aacagttctt ttaacgtgta
    12061 cctcttccat tcaaggttat tgccgcttac gaaaacagga gcatcttcct gcatgaattc
    12121 gctcattacc tgacggcata tcccgcacgg atatgcatag tcaaacacat ctccggttat
    12181 ggctatagcg gtgaccgatt gctctttttc gcttattgcc tttacaatag ccgttcgctc
    12241 ggcacatatg gatgctccgt aagacgagtt ttcaatattg gcacccgaat aaatcttccc
    12301 gcttgatgcc agaaccgcag cccctatctt tatatccgaa tagggtgaat aggcattttt
    12361 aacagcttct tttgcttttt gttttaattc ttcatattta tctttcatag tttaactctt
    12421 tcagaacctc ttcctgaatt gcaagcatta cttcttccga ccgtttatct tcatggtcgt
    12481 agcccaaaag atgcaacagt ccgtgacttg caagaaacac acactcccgt aacggactgt
    12541 ggttatagct ttccgcctgt gaatatatct tacttgttgc tattaaaata tctcctaaga
    12601 acaattcgtt attctcgtaa tcaatgtcgc atttgttata aataatagta ccttctttac
    12661 agttgagtgc gggaaaagac aatacatcgg tagtcttatc catattcctg tatgtcttat
    12721 ttacctcaag catatattca tcgtccacaa actccaggta aaaagatact gttatgtctt
    12781 tatcatctgt ttcatgtctt gccagtatac tttccgatac ttcttttacc ggccgggaaa
    12841 acagggccgt tacttcatcc ggtccttcta tatcaatcct cgtcttcatc ccggtcatcc
    12901 tccttacggt ccaagcccat gctttttaca gcatcatccg actgctgatt gggatatttt
    12961 attctcttat gaaaatatcc ggtaaacact ttcatgaagg caaacagtat ttcatccagc
    13021 tcttttaatg taagatcgca ttggtcaaac tgtccgtcat ccctctttgt tttggccact
    13081 cttcttatgg tcttttcaat ttcgataata ttggtgtttt ccatagatct tatggtggct
    13141 tcacaggcat ctgcaagcat gacacatgcc gattccttgg ttctgggttt gggtccggaa
    13201 tatctgaaat cttcctctat aaccggttca tccgaagcat tcttggcttt atgatagaaa
    13261 tactccacca atgtcgttcc gtgatgctcc actatgatat cccttatgga gggcggaagc
    13321 tttgcctgtt ttgccaaatc cgctccctct gtggtatgac cggttataat tgtggccgac
    13381 agttgcggtg ttatcgtatt atgaggatta cctgtcttgg aagattgatt ttctccgaaa
    13441 aatgcgggtc tgttcagctt gcctatgtca tgataatatg ctccgactct ggctaaaaga
    13501 ccgttggcac ctatcgcttc ggccgcttcc tcggcaaggt tacccaccat cagcgaatga
    13561 tgatatgtcc cgggagcttc catcataagt ctcttcaaca gaggttttgt gggagatgcc
    13621 agttccataa gtttgaaagg agtgacaatg ttaaagaata cttcaaatac cggcaacagt
    13681 ccgaggcata aaagtgaaga aaccaccgat gcgacaaaca gagctataat ctcgggatag
    13741 ggaatactta tcgttacatc ggaagttata agtttgtatg acatgtatag agcgacattt
    13801 atcacaccta atgctattcc ggaaatagcc agtcttattc ttgcggaagt ctttcttaca
    13861 aaatatatgg tggcaatgga gcctattaga agaattgcca gatatataag gtctcctccc
    13921 acaatgtagc aatagaatat cgccatagga ccggaaaaga taaatgaaat gttgtcggat
    13981 acaaggatgg aaagcatcat gggcaacaga aaatacggga tatacatata gtattcatga
    14041 ggtatataca gagcgggtat atataccata aagaacaata tgaccattat gaatatggac
    14101 ttgggattaa gtctcacggt ctgtccgtat atctttaccg atgcgaaaga agctaaaaga
    14161 cacagcaaaa acagcacggt agcccctata tacatataca tctcatcgga tgcggcaatc
    14221 atacctcttt cgtaaagcgc caatatatcc tcgttctcca gtttttcacc tttctggtat
    14281 aaaaccgccc cttccatgat taataccggt gagttggtta taacatcgtc atatactctt
    14341 tgccgttcct tttccgtcgc accggtatct attctgctgt taatctgtat cagataggat
    14401 ttaagaacat ttgtaagctc ttttaaatta ttgtcggtaa ccgattgatt taccgcaact
    14461 tcaaattctt cctgatgttg tgcaagattt tcgtaaaata tgtatttatc cataaatacg
    14521 gagaattgcg cacggattac cgttttgtaa ctgtcatagg aagtgctgtt aacatcctgc
    14581 agtatggcct ttgccagatt gtgcgagata tttatgcttg acggcaacat acctgtatac
    14641 tgttttacca gaccgtcaat ttccgcattg tattgctgcg aagtgagaaa cagttgctca
    14701 gtcttttcgc gatagcggtt ggttacatca aagaaagtga atatattttc atataacgcc
    14761 acttgtgacg aatttatcct gacggcaatg ggttctaccg agtctttggc tttgttggct
    14821 tttgctattg tggcttcctt gtcgtagtag tcatgtgaag ctacaatatc ggttgtggct
    14881 atgttatgtc tgtctatcag atgtcccgca tcggacacct tttgcaatat acccaataac
    14941 gcaaccatta tcgccaacag aagaattaca acgctgtaca gttttgtctt aaataaatct
    15001 ctttttgcag ctttggtttt gttcatttgc gctcaccttt tttactctcg gcacgttcgt
    15061 atgcatttat tatcttttgc accaactcat gtcttactac atctttaaaa gtaagattga
    15121 ctatgccgat atcttctatg tttttaagta tttgtacggc agtgataagt cccgattttt
    15181 tgtctctcgg aaggtcaatc tgagttatgt ctccgtttat aaccatcttg gaaccgaaac
    15241 ccattctggt taaaaacatc ttcatctgtt cctgtgtggt attctgggct tcatccagaa
    15301 tgatgaatgc atcatccaat gtacgacctc tcatatatgc caacggtatt atctcaatta
    15361 gccctttttc tttgtttctt gcaacattgt ccggccccat cagttcaaac agggcatcgt
    15421 acaacggtcg taaataggga tctattttag actgtaaatc accgggcaaa aatcccagtt
    15481 tctcacccgc ttccacagca ggtcttgtaa gaataattct ttttaccgct tttgctctaa
    15541 aagccgttac cgcacaggct acggccaaaa atgttttacc ggttcctgca ggacctatac
    15601 cgaaggttat accgtttttt cttatggtat cggtatattt tttctgtcct aatgttttgg
    15661 ctttaatagg tgctcccgaa gcattgagac atatatagcc ggtaggcagt tggtatatct
    15721 cgtctttccc gtttttcaca aggtcggcaa gatatactat attctgcttg tttatatctt
    15781 cccccgactg agccatttca atcagcatgt ttataagttt ttcggtagcg tttacctctt
    15841 cggcttcccc tttcagtttc acttcgcttc cgcgcacaat tacagagaca ttcatcatat
    15901 tttcgataat gttcagattg ctatcggaac tgccgaatac actcatcata tcacttaagt
    15961 tttcaaaata tataagtctt tccattaact cccgcgtatc aattaactta aaccttaatc
    16021 ggtctgtcac caaattatat catttatatt gacaacaaat caaaccgtac ctaatccagc
    16081 ctgtttttca actttgaatg cagctgatgc atcgcataaa cagtgcttgt agacagatga
    16141 tgctctatct tggtagtttc cgcttctgcg gtctcttcat ccaccattaa gatgtctttc
    16201 agaaatttca cgattatgag gtgctttccg gttgcggctt ttacggcttc aatgcccgca
    16261 tcggtaagaa atatcggtcc gtattttacc tgtgcacagt aacccaactt ggacaatttc
    16321 attactgcat tatttgcgga tgcttttctg actttcatct tatccgcaag atcggtcagt
    16381 ctcacacctt tattggtata ggaaagttcg tgtatacatc gtaaatagtt ttccatggat
    16441 tccgtcaatc cgctcatagt aacaccgccc tgatatcaga atttattgat taacatgatt
    16501 ctaccaaatc tgccacttat tagcaacttt taagttgcac ttgttatttc ctgctttatt
    16561 tatcctacct ttatttactt aatctatata gttaacgata acctcattaa tagttgagtg
    16621 taaacacttc ttttagttct ttcaatgtct ttgcggcaac cgacctggca tactcgcttc
    16681 cggttttgag cagtttcata acctcctcgg gattctcttc atattctttt ctgcgttgtc
    16741 ttataggctc cagtgtgttt tgtagtatat tatttaagcg tttcttcagt ttaacatctc
    16801 ccaatccgcc tcttttatag tgttctttca attccttcaa ctcttcctgc tcagggtcaa
    16861 aagcatcaag atatttaaaa acggtattta tctctacatt tcccgggtct tccacgcgaa
    16921 tatgattagg gtcggtatac atgctcatta ccttcgcgct tataacatca ggctcatcat
    16981 ttaaaaatat agcattattc atggactttc ccattttact taaaccgtcg gtaccgggta
    17041 aacgtaatac tttggaaagt aaaggcttgc actctctcaa aacagcggta ttaaaatgat
    17101 tattaatatg tcttaccact tcatttgtct gctccagcat aggcaattgg tcatcgccga
    17161 ccggtactaa attggctttg aatgcggtaa tatcggcaac ctgactgata ggataattca
    17221 gaaacccgac cggaacatta tatccgaaac ctttctgttg catttcatcc tttaccgtgg
    17281 gattcctttt caatcgggct aatgtaacaa agttcatata aaataccgta aattcggcaa
    17341 gttccggtac caatgattgt ataaaaaaag tgtttaattc cggcttaagt cctactgcaa
    17401 gataatcaaa tgcaacctca tatacatttt cacttacttt tttcggattg tgtgcattat
    17461 cggtaagcgc ctgtgtatct gctatcatta taaaaggttt atcatattct tcctgtattt
    17521 ccactctttt tttaagcgaa cctacataat gtcctatgtg caaaggtccg gttggacgat
    17581 ctccagttaa aactatatct ttcataaagt gctcctttcc tctctttgtg tatgctacac
    17641 aaatacattt tgggcaaact agtacgctta tatcactaat ctatcgcagg gttctgcgtc
    17701 agatattcgt ttataaacgg gtccaaattg ccgtccataa ccgactgcac atctccggtc
    17761 tcataatcgg ttctgtggtc ttttaccatg gtatacggac agaacacata agaccgtatc
    17821 tggcttcccc aacctatatc catctgattt cccttcaggt cctcaatctt ttccttatgt
    17881 tctctttcct ttatttccat aagtttggac ataagcatct tcatggcttt atccctattc
    17941 tgatgctggc tcctttccga ctgacaggct acaacaacat tggtgggaag atgggttatc
    18001 cttaccgccg aatcggtttt gttgacatgt tgtccgccct ttcccgagga acggaatgta
    18061 tcaacggaca aatcttccgc ctttatcttt acctctatct catcggaaat ctccggtata
    18121 acttcacagg atgcaaagga agtatgtctt cttcccgccg aatcgaaagg agatattctg
    18181 accagtctgt gcacacccga ttcgcctttt aagtagccgt aagcatattc gccttccaca
    18241 aaaaccgata ccgattttat gcccgctccg tcgccgtcaa gataatcggt aacggtggct
    18301 ttaaaccctt tttgctcgaa atatcttgta tacattctaa acagcatctc cgcccagtca
    18361 caggcttcgg tcccgccggc tcccgcatgt aatgtcagat atgcgttatt cttatcattg
    18421 gtaccgttaa gcagagagta catgcgtatt tcggtatatg tatcgtttaa ttgctccata
    18481 gcttcaataa gctcattgct caattcctca tcttcgggat gctcgtcaat gaactcaagc
    18541 aatataaagc agtcatccag tttggtttgc agatcggctg cctttgccgc acggtctttc
    18601 agcaccttga ttctttgaag cactttttgt gagttctcgt tatcctgcca gaaatcttct
    18661 tttatagtct ctttctcaag agctgctatc tcttctcttg tgctgtcgga atccaggctc
    18721 aatatgagct tatccgattg ctcctttatt tcctccaatt gatttcttat gtcattaata
    18781 tcaatcatta aagtgtgttt acaaaccttt ccattcgttt tagggcttct tttatattct
    18841 ccaacgaact ggcatatgtc gcacggaaat agccctctcc ggctgaggaa aatgccgttc
    18901 ccggaactgc cagtacctta tgttccataa gtaatctgtc gcagaacctc tcggatgtca
    18961 gtccggtctt tttgatgttg gggaatacat aaaaggcacc ttccggtaaa aaacaatcaa
    19021 tacctatttc aataagtccg ttgtatatgt atcttcttct tgcatcatat tgttttctca
    19081 ttctttccac atcggcttca cactgttcca gagcatctat tgcggcatac tgtacaaatg
    19141 acggagcaca cattatagca tactgatgta ttttgagcat ctcggcgact atatctttgt
    19201 gtgcgcatac atatcccagt ctccagcctg tcatggaata tgatttggaa aatccgttta
    19261 catatattac tttgtcgtgc atgttttcca actcggcaaa ggagaaatgc tttgttccgt
    19321 atgtgaggtc ggcatatatc tcgtcggata tgacaactat atcttttgtc gacagataat
    19381 cggctatggg cctcaactct tcctttgtca tgatggcacc ggtcggattg ttggggtatg
    19441 caagaatgag tgctttggtt tttttcgtta cacactcctt taactgctcc actgtcagct
    19501 taaaacggtt ttcttctttt aactgtaacg gtaccgccgt tgctccggca aagtttacac
    19561 atcctttata tgccacaaaa cacggctccg ggataataac ttcatcaccc ggtcccgcta
    19621 tgctccttat ggctatatct atggcttccg atgctcccac cgtaattatt atctggttta
    19681 agggattgta atccagatta tacttttttc tcagcatatt acatacggct tgcctaagtt
    19741 ctaataaccc cgtattggat gtgtaatgag ttctgccctt ttccaatgaa tatatggcgg
    19801 attctctgat ctgccacggc gtgacaaagt ccggctcccc cactcctaaa gatatgacat
    19861 cactcaatcc gctaagcata tcgaaatatt ttcttatacc ggaaggagga gtgtttctca
    19921 cctcttccct tataaattct ccgggtttct tcacagaact atagcctctc ttctgtcttt
    19981 ttcctcttct tcgaatataa ggtcgttttc cttatacttc ttaagcacga aatgcgtgga
    20041 tgtagcggtc actccctgta tggcggaaag cttctgagcc acaaacaatg ccacttcttt
    20101 taaatcggac ccttccacaa ccgccgctat atcaaaagct ccgctcatga ggtaacatgt
    20161 tttcacctgc tcatagctgt atattctttt tgctatttct tcaaatccgt agtctcgctc
    20221 cggagttacc tttatctcga taaaagccac aactatatta tcccgtgctt tttcccagtt
    20281 aataacggcg gaataacctg caattacctt gtcccgctca tactgcttta ttactttttc
    20341 cacttcttca acgcttttac ccgtcatgac cgcaagttct ttcggggtaa gccttgcatc
    20401 ctgttttaat aattccagta tatccataac ttttcctttc ttataaaaag aggactgaca
    20461 gtcctctgag aataccgggt ttcaagcaac cggctattct cttaatctga tgtattttac
    20521 agtcatttct cgtcggaacc gatagtttta tccagattct ccacaaactt ttccaaaagt
    20581 tttgctgcag atgcctgcaa tgtggccagc tgccttttta tatccactat cttttctctt
    20641 tcttcttccg ctcttacttc cagttcgccc acttctttaa tagcctgttc cttggcttcc
    20701 tttataatct ggtcggacat ttcattggct tttatcaagg cttttgtaat gccggctttt
    20761 tcctttacaa gagcatcttc tttactcttc aattcatcat atgcaagctg caactccgcc
    20821 gctttcttgc tgagttcctc gatggtggct tctttttcgg ctattttggc ctcatattgt
    20881 tccatcatat tatctatata cttgttgact tcatccttgt tgtaaccgct cattgcgtta
    20941 cggaatcttt tttttgccat gtcacacctc ttctaatgat gatatttatc tcgttttaat
    21001 tttatatata atcagcccga atagcaagca ttttagaata tacctgtacc tttatgctct
    21061 tttctccatt tgtttgatgc atcttcccaa atatcgttgg gtatcgggtc gacattttca
    21121 tatcctttct cttttgccat ttgcttagcc atatgtaaaa aattaatact gtcatactgc
    21181 ttggtaaaga aagaatcggc tataagttcc atccagaaag tatgctcata actgtcaata
    21241 tcttttaagt aatactcctt aagcttggca ggattatacg gtatgtatac aggtcggtac
    21301 tcaacgagtt gtttatcctg tattgatatt atccccatac tgaatgcatc ctctcttttg
    21361 taaacactga ttgcgggcgg tatccccaaa ggtccgggat ttataaaaaa gcttcccatg
    21421 aggtcaaaat gataggactg atgcgaatga cctaaaagat acagtgaaca gccgtacctg
    21481 tcatgcatat ccataaatac atcgatgttc cagcaaggca gtatgaaatg tcctacactt
    21541 atatgagagc cgtgaacgat aagggtgtcc agaccgaaaa gattcagtct tttcatatcg
    21601 ggcaatcccg aaatatactt aagtcccgca tcggtaagat tcttgtgtgt ctttaccaac
    21661 gattcgcact gatagttgtt atcccactgt tttcttttac cggcatgata attcaagata
    21721 tattcttccc ggttacctct tacaacatgc tcggtaagat tcatgacggt atccaacact
    21781 gcctgcgaat cgggaccgtc ggaaatcata tctccgagca ctatatattg ctcgacatcc
    21841 tgtgctttac atatgtcaat tgctttctga agcatgtcaa gattgccgtg tatatcggaa
    21901 aaaactgccg cttttttcat ccggtaatat attctttcgc tattttatcc atttcttttt
    21961 cgtctttagg aggagcaatg ttgtcaaaca actgccacaa ttcttcgtgt tcaatttttt
    22021 ctttgggacc caacagaaca aggggtgaaa gggtttccat ctcggtcatg aaatcgcaag
    22081 tataggtctc atacatggat ccgccgtaat cgggataaag catgtcttct ttaggcgaaa
    22141 actttttcac aaacatattg ttccggttga aatatgccgc ccaaccgtcg gtattggata
    22201 tgcctatttt aaaagccttt tttgcttttg aatcatgtgc aaggaaaata tacttacttc
    22261 cccaacgcac tcttttatca ttcatatcgg tatacggcca caaagatatc attctgttgg
    22321 gtaaaagacc ggtatccagt gtttcctgcg gtaccacttc cagtcctccg ggtgcaacta
    22381 cagtcagcgc ccatgccgca agctctatat cccacagatt acagtttgtt aatatgtggt
    22441 tgatatatac atcggtccct ttatccttta cggtgatttc catatccttt tttatcattg
    22501 tggttgtttc cgcaggttgc gatatcctta taccgttttg tattatcttt acatccacag
    22561 cttcattatc gggaaaatag gtgcgcactt tatcttcggg actgtgccac agcctgtgtc
    22621 ctccgtatat tctccactca tctcctccgg tcttacccat ctggtcggct ttttcacaca
    22681 agacattttc tttgcctata aatccgtacc ggatgattct cggaccgaca tccgatgtaa
    22741 ctatcaactc aatttcatcg ttatatattc tgtaacaatt atcccaaccg ccataaggga
    22801 ctttctcaat ccgtaccgac attttttcac ctcgtatttt tatataactg ttatactatg
    22861 cctttttaag gcaatgtcag attgtgcaat atatagtaac ctgtattatt tcttttttcc
    22921 ggtaaaccag tgaattatag gtataaccag gaatattatc caacccggat gccataaatc
    22981 ccatataaat cccatagcca gatatatcag gactatgatg agtgaaaagg atatagtgcg
    23041 gaatttaata gcattaacca ccgcatagta taccggcacg gtcagaaaaa caatccaacc
    23101 gggatgccat ttgccccata taaagcccat aagtaaaaat actatggtag caagtacggg
    23161 ataggggaat gttgcggcat cgcgtctgtg ataccatttg ttcggtttac attcaggctc
    23221 aaagcaattg tcctgttgct ccttgtttac attatcggtt tcttgttgat tcatatcagt
    23281 gttcctttcg ttaatactca cttatattat actacacacc gacctaaatc ttatactgat
    23341 ctcccactcc gtaatgttca acaacataat cgatatcttt atctcctctg ccgctcaagt
    23401 tgacaagtat ggaattggcg gagttctgtc ttgccgattt aattgcatat gcaacggcat
    23461 gggcactttc caaagcgggt ataattccct catacctgga aagcatgaag aatgcttcta
    23521 tcgcctcgtc atcggtcact gaagtatact ccactctttt catatcatgc aagaaggcat
    23581 gctccggacc taccgcagga taatccagtc cgcttgcaat ggaatatacc ggcaacgcct
    23641 ctcccttttc atcctgcaac agaaggcttt caaaaccgtg taaaactcct ttgcttccga
    23701 aggttatact tgcggcatta tctcccactt tgggacctct tcccataggc tctatgccca
    23761 ctattttgac gggatcattc aagaatgccg taaacattcc catagagtta cttcctccgc
    23821 ctacacatgc acaaacctcg tcgggatgtt ctcctgtcat ttccagatac tgctccctgg
    23881 cttctcttcc cacaatcatc tgaaaatccc ttaccatcat agggaaagga tgaggtccta
    23941 ctaccgagcc tatgcagtaa atggaatctt tgtagttttt catatacgat tcaaatgcgg
    24001 aatctaccgc ttctttaagc gttttcagtc cgtgggttac cggcactatt tttgcaccca
    24061 gaattttcat tctggttaca ttgggagcct gcttagctat atccacttca cccatgtgaa
    24121 tttcacattc cagtccgaag aaggcggcag cggtagcaag tgccactccg tgctgtcccg
    24181 ctccggtttc agctatcaac cgctttttac ccatgtattt ggctaaaagt ccttcaccca
    24241 tgcagtggtt tagtttatga gcacctgtgt gattaagatc ctctcttttt aagtatatct
    24301 ggcatcttcc cagtttattg gaaagccttt cacaatgata taccggtgtg ggtcttccct
    24361 gaaattctct tcttattctt cttaattcgt ttatgaacga tgccgagtgt gctatcgtca
    24421 tatatgcctg gtcaatttcc ttaaatgctt ccataagttc cgggggcaga taagctccgc
    24481 cgtattgacc gtatcgcccg tttttgtcgg gatagtattt agtgtagttt gtaaaatcca
    24541 taatattgtc ctctcttacg cttatttctt attcaacatt gcctttctgt aagcttcccc
    24601 ttcccagccc atgataaatg ctatatcctc atcttccatg gtatatatgt caaggttatt
    24661 cttacaagcc tggtccatta tgaaaagaaa tgcacagatg ctttcttcca aagtaagtgc
    24721 ttctttatat gaaacatggt accgggttat tccggttttt aagtttattc ttgctttttt
    24781 ctcttcacct cttgtagtta ttgctcccaa aaggtatgcc gcctgatcgg gataaagtat
    24841 attggaatca aaccattcat cggtataatc tttccctttc agataggaga tgagtctcgg
    24901 tgtatccgac tggtataccc cctcggataa ctgttttacc gctacgttac cgtacggttt
    24961 tgttatattc aatctttctt ttattctgtc ggaaacatat tcatttatct tttccgcttc
    25021 ggccatagtc ttgccggtga ttataactcc gtggcttccc atgaatataa cggagggaat
    25081 acatcccatt gtctttatct tcttttgtat ttccagtgtg agataaaaac ccggagcaat
    25141 gtacggaagc cacaggtatc tgtaaggtga tttctcaaag agttgctttg ctatgctttc
    25201 tccctgcctt gaacagcata aaagattgct gtaaaccgaa tgggtatgga taacatactt
    25261 ttctaaaagc gaatggaatc ccgcttctat tgacggtctt ccttcattga gcccttctaa
    25321 ttttaccata ttgtctttaa cgaactgcga actttcttta tagaaatccc ttttttcgct
    25381 taaatccacg gtgtcaaaat agtgacggac ggatttataa tctaccacaa cataaccatc
    25441 ggttgcggtt atgtcgcaaa gtctgcaacc ggatgctttt accgccataa gttcatcatc
    25501 caatttcacc gaagtgtttc cgccgcctcc ctgtgtgtag tcaggcatac ttcccacctt
    25561 acgggacatt tctatcattt cttttatgtt tttttcgtac ataataatct ccttcatgtt
    25621 ttttactgac gggattctat tgattgtata tctattttag cccttgttca atatgttttt
    25681 caggtaatag tcatattttt cttcccaaag ttctctgttt tcggggaaat atgacttaac
    25741 ttcaaaactt tcttttataa ttattctagc ctgcttgagg tcttttatct tgcctaaagc
    25801 tataagttgc gccgctatat tcccgacagc cgttgcttca acgggacctg ccgtaacctc
    25861 tatacccaaa gcatccgccg taaattgcga tatcattgca ttcttgcagc cgcctcccac
    25921 catatgaagt ttgggtatcc tgcttcccac tatttcgcaa agtccgtcaa acgcataacg
    25981 gtatttcaat gccaggcttt ccattataca tcgtactatc tcggctttgt tcttgggaac
    26041 atactgaccg gtccgtctgc agaaatccgc aactctgtcc ggcatatctc ccgggggata
    26101 aaagtcctga tggtccacat caattaagca tgcaaaaggc tttgctttcc gtgcagccgc
    26161 ctccagctcg tcaaaggatg caagattccc ttctttattc cattgtcttc tgcattcctg
    26221 atatatccat aagcccataa tgttttttat aagtcttgcc gttctgttta ttccgccttc
    26281 gttggaatac tcgtgtttat atgaaagatc attcactatc ggcttgggca gttcggaacc
    26341 cagtaaggac catgttcccg aggaaagaaa tgcaaacaga tcggtgtctg cgggagtagc
    26401 tatcatggcc gacccggtgt catgtcccgc tacggcggta acggttatcg gttgcagtcc
    26461 cgtctcatta caaacctgct ctgaaagttt tcctatgact gttgcactgt ctgttacctt
    26521 ttccaatatc cttacaggta ttcccagttt ttccagcaat tcgtaatccc agtcaccggt
    26581 acgggtattc agcatctgtg cagtgcttgc catggagaat tccgccactt ttttgccggt
    26641 gagaaaatat gaaagcaaat ccggcatgaa aagaagcctg tccgctcttt tcagcaactc
    26701 cggtcgtttt tttaccaacg aatacaactg ataaagtgta ttgaattgca taaactgcgt
    26761 tcccgttctt tcgtaaagat gtcgcttttc taccttatcc cacacttcct ccataatacc
    26821 gtcggttctt ttatctctgt aatgtacggg attttctaaa agccttccgt ctttatcaat
    26881 aagaccgaaa tccacccccc acgtatcaat tccgatactc tctatatcac cgtgtcctgc
    26941 tttctttact tcaataagac ctaccttcat ttcatggaaa agacgtaaaa tatcccagta
    27001 cagggtaccg ttcagattca cgggatcatt ggaaaacctg tgcaattctt ctatgtgtat
    27061 cttctttccg tcatatactc ctaagattgc tcttccgctg cttgcgccga aatcatatgc
    27121 caataccttt atcatcatta acctccaatg attttagtgt ataagtatct gttttatgcc
    27181 ttgtctatcg ctttaatata tatgtccagt tgtgcgtttt caaattcaaa agccaacttg
    27241 gctaaaggct cgagattatc ttccgaagca aacaactcga atgcacgcac atactcgata
    27301 tatctgttct gcggtatgta tatgggcagg aaaccttcct tcataaggac ataattcatg
    27361 ataagctttc cggtcctgct gtttccgtca ataaaaggat gaatcttcat gaacatggca
    27421 tatgcataaa tggctttttc cgtaaccgta tcatattgca gatagggcag atcgagaata
    27481 tattttttca ttttgtaata tgctttttcc ggtgacggcg gaacatgctt tcttccggga
    27541 atgataagat tgacattgcg atatatgccg cctacggaaa tgtcttccac aactatggca
    27601 tttatctctt ttattgcttc ctcatcaaga tatgcgttct tctttaagta cattttcata
    27661 tacttgaaag ctctgctgtg gtttatcgtt ccctggacat cttttgtttt ctccatgtcg
    27721 tcggctttat catttaagat agccatgacc tgatcccttg acagttcact tcctttaatc
    27781 ttgatggatt cgtaagcgta gtcacactcg aattgttttt ccaattcctc gattttttca
    27841 ggggataatt cgggcaattt gccgtttacg taatctcttt tcctgcacaa ttcctggtag
    27901 ctcaattgcg gacacctctt tcttatttat tataaaacat agcctttgtt atatcttcgg
    27961 tcgcttgccg ttcgtgattt atcaaggcat atgttgattc ccttaaatta tcgaaaaacg
    28021 aatctctcat ggctttgcac acaaaactct gcctcggtga gtttatatcc tctccgctta
    28081 tctggaaaaa tccgtaccgc tcacataacg ttctaaccct tatcagttgc tctttggtat
    28141 ttctcgaggg catgtatgtc accgcatcga aatccagttg cttcaacact tcaaaaagct
    28201 tatccagata agagtcctcg aatttctgcg gtttcttgtc tccggtcacc gaacttgcca
    28261 catctcctaa atatgcatat gccgatatgg ctttggtctt tctgctcaaa gatattacat
    28321 cgtgtatatc gggacactcg tcataagcgt ctatgtagaa cagtgaaatc aaatcacttt
    28381 ttatcgctcc caaaaggtcg tactcataca ccggattgga cacatcgctc aataaagctt
    28441 cattcttaag gcttaaaggt aaatttaaag tagtcttgta gaaatctata agtgcttttc
    28501 ctcttttgaa acgttctatc attttaagtg caagtgcata agatatgtgt ctttcggtta
    28561 ccgttccgcc ttcatgatac aaagataacg gagcgacctc tttatcaaaa tccaatgaaa
    28621 tatcatattt attaagcact gcatttatct tatcgcacat ggcacggttg cggtcatttc
    28681 tggccgctct gtacggcgcc atgaaatcct gtattgtatc tatatactga tgaggtattc
    28741 cgtgaagagc gacataggca acagaatcct ggtcgggatt gtttatcctt aggccgttta
    28801 acggcgtctt atccatacgt gcccttacct caataccgca ggtaatgggc atatctaaaa
    28861 tcttacccgc ttcaataaac tctttgcacc ctgcaaccga atcatgatcc attatgcccg
    28921 cagtcttaag accgttcatg taagccataa acagagcttt tgagggactg tacggtgaaa
    28981 aggaatatgc ggtatgtata tggttgttta catcgttata gaataccggt ttaggcatat
    29041 cgcctttatc tacggcattt ttcaacaccg acagactgtt cagcctttcc gcctttttag
    29101 gattattcag attttcaatt tgcagaagga agaattcatt catatttatc gcatttcctg
    29161 tccgcctgtt acattaattg cctgtcccgt catatagccg gacatatcac tggccaggaa
    29221 aaccatgaca tttgcaacat cctcatattc acaggatctt cccatcggta cctgatcaag
    29281 gtacttctga cggacttcct cttcggttat tccctggttt tttgcatact gcttaaaaag
    29341 actgtcaacc cataacgggg aatttaaaag gttacccgga catatggcat tgaatcggat
    29401 attgtctttt gcatactcca gagcaagact ctgagtaagt cctataccgc cgaacttcga
    29461 tgcggcatat gcactgtttc taaaagagcc ttttttgccc gatttactgt taatctgtat
    29521 aaaagatgct ccgtttttat tctttaacat atgttttaca gccgacttgg cacatatgaa
    29581 atatccccaa agattgacat cctgcaccag tctccatttg tttacatcaa actcatcggt
    29641 gggaccggat ataacaatgc cggcattgtt gacaaatata tcaattcttc cgaaggtatc
    29701 cacagccagc tgagtcattg catctacttc cgtctctttg gtgacatcca ctttgcaggc
    29761 tacggctttt ttcagcggct ttgccacttc acatactttt tcgtaattca aatccgccaa
    29821 aacgattgaa cacccttctt tatccagtct ttgagccaat gcctcgccca aaccctgtgc
    29881 ggctcccgtt atgacagcaa ctttattatt caataatttc ataaaaaagc tcctaattgt
    29941 tattcttacc tttattatat cccaacggtt ttcaaaaaca tataggattg tgtcttttta
    30001 actaaagaaa accgctattg tctcaaaaaa taccagtata taaggagggc ggacagtgca
    30061 attataagta tgaccaatat aacggtcttt ataatttttc ttctgaattc ctgcttaccc
    30121 tgcccgacat ctttgtcatc tttatcactc ataaattgct ccctttagtt ttctcattat
    30181 catcaccatg gcataggtgt ccaacttaca gtattccagt aactgattcc gaacctgctg
    30241 tttttcctct tccgacatgg aaggcatttt tctgaacgca gccattgcct gatctcctct
    30301 ttttactcct tcaagatttt cgtaatccaa agtataatca tcgggaaaaa gagcgggcag
    30361 taccgctttg atggaatagc ttccctgcat ttccttatca taataccagt ggtttttaaa
    30421 gggtatcata agatccagca tattgtcata tatatccgta agctcatccc gaaggtaagg
    30481 aaaataatcg gcaaggtctc tgattacacc tttttcaaac gccatattat atgcaaccga
    30541 gcaggcgccc ttaggtatat cgtttaccag acgcgtggcc aattgttctc tgcagtcaaa
    30601 tccgtccgat gcaagatact ccttatgctc cagcctgccg tcctcatatt ctatatgcaa
    30661 agaatattga aaaggtgtct gctgatacgg cttcatgccg tcatacggcg gtatggcata
    30721 ctgtatcgtt tcaaagtcta aataatacag aggataacgg aacttgctta caaatcgacc
    30781 gatctctttt ttatctataa tcttttcttt cagaagctgg tcttttacaa ccggtctcag
    30841 ccgtgaaaat acaactcttt cattaataac atcggaaaag gaatatagtc ctctgttata
    30901 catgtccagt ttattcttct tattaacatt cttaagcata aaaatattct cttcgggcag
    30961 atgacgtgtg cagtaaccga aatatccgca aggataagga gaaaaacagt aatccgcaac
    31021 agatacatcc ggctcaacgg taaggtctaa atcctgtgat aaaaactcta aaagctgctc
    31081 tacggattga tgccgtaaaa aagcctgaca agtcacatca gtgacagaaa acagctgagt
    31141 caaatccagc tcgccttttc tgacataact tgaattgagt gttattacat ttaccctttt
    31201 aacattgaaa ccggaaagtg acagaacata actctgatat gcggcatcat caaggtatac
    31261 atctttaaca cttgtggaac tctttacctc attgatgata acgccgtcat cacatacttc
    31321 caatatatcc acgctcaaaa agcgtccttt gtaagcaaaa gaagcttcgg caatatatct
    31381 tttacccagc ctgatatatt cacttgtcct atttatcatt tgcaacttat catccgagta
    31441 ttctacaacg gatacattat caaaaagctt ttgcgccaat tcccctactt cattgccggt
    31501 ttccagcacc gcttcatcca ttaccgaatc atcaaactct tccggcttaa ctctgtccat
    31561 ccagtacatt tttatacact gtaccgctct tacatatctg gattttgaaa cattgaaatc
    31621 tatcataatt tcttttttat atatgtctca tcatattcgg ctatttcgca attgtccaat
    31681 aaataactgc ggtaatctcc gttttcaggg gcccctttga aatattgagc aacggctatc
    31741 gatactccgc cgtgtgcaac cagtaagacg gttttgccgt aatgcttttt aataatttct
    31801 tcaagaaaat ccacaactct tccgtaaaac gcaatatatg attccacagt ctcaagctga
    31861 ttattcatat ccagtgacca tactttttta taatattcgg tatcctgcct gtatgaaacc
    31921 ccctcgtact taccgaagtc tctttccgca atcctgcggt ctataatcat ttccacatct
    31981 ctgccttcac atactatgga tgccgtatgt cttgctcttt ccaaaggcga aactatcact
    32041 tcatcaaagc tatgcttctt aagcatctct ttaaccgcat gtgcctgttt tattcccgtt
    32101 tcattaagtg gtatatccga agagccctgt actctttcct gtatgttcca atcggtctga
    32161 ccgtgccgta taaatagaat cttcatagga atatgataac ataaaacgga ctatcagtca
    32221 ccgattttcc tcacctttat atccccgctg ccgaattccg atttcaagag atagtaatcg
    32281 ggagggttac tgataactgc agaggacaga ataacatagt cctccagttc cgttacataa
    32341 aatgttaagg agtaatcctc catttccaca tcaaacaata cactgtctat cagattgtac
    32401 tgcctgtcaa aaatgtaaaa catgggagaa acggaagatt cttttgtttc aaaaaactga
    32461 tataagtaga tgtactcatc gttatatgaa gccacataca agccgtcacc tataaaatcg
    32521 gctactttct ccgttattcc cgtttcatat gtgtattcat aaaaaccttt accgttcaga
    32581 taccaatata ctttatcgtt atcggtaaat acattttcat aacaccactc ggtgtttaca
    32641 aattcactcc actgtcctgt ctccattgaa tacctgcaca accggtattt tgtttcggga
    32701 aagtaggtgt agaaatatat atacccgtcc aaagggtaaa accaggacaa attacctaca
    32761 gcaatacctt tatcctcggc atccgctatc tttacacttt cactgttgtc aaagaccgcc
    32821 gttttatata tcacattatt tgtgttgccc aaaagagcaa tagaaccttt gaaaaagtac
    32881 aaataaccgt gttcaaagaa gccggtaagc ctttcctgca tagtggaaat gagttttacg
    32941 ggaatatgat tgtttttgtc catattcatt ctgaataacc agtagttccc ttgatgaatg
    33001 ccgacatagt aaatatatcc gtcataatat gcaattgcaa tttttgtcgt ggtctttatg
    33061 catgcaatca ggcttggaac acaatttaac atattcggaa ataccgtcga aactgaaata
    33121 gacggagcag tctttgcaat ttaaatagta gatacctttg tgcgtttcaa caaacaaaga
    33181 aataaccgaa tccgactgcc ggaaacgcgt aaatctgttg tcgtttatat caccgttaaa
    33241 aacattgctt gtttcagtga tattatccgt atcaagattc ttctcattaa aagttttaca
    33301 ggaggataat actataagca gagtaaataa cagaaatagt gcgattaatc tttttttcat
    33361 gcggttacac aaccaaaacg ttgtcttttt tgcatttatc taaaacttta tcatcgggca
    33421 tggtgtcgca aacccaaata tccacatcct taagccggca gaaagtgtat gctttatcct
    33481 tgtctatttt ggtagaatcg gcaagcatta tggtttttct ggccttctcg ataattctgg
    33541 ttttcagttt ggcatcatgc atattggaaa cggaaaaacc cttctctgcg gaaaatcccg
    33601 aagctcccat tacagccaaa tcaatgttca ttatctccaa catttgcatg gcttccggac
    33661 cggaacatgc caatgtattg gggcttatcg ttcctcccag caatataacc gagttatcgg
    33721 tggaagcaag tttcaaagcg gtgtttaccg cggaagttat aaaggtgcag cctttcatat
    33781 cggtaagaag attggctaaa cccataacgg tacttcccgc atccaaatat accgatcggt
    33841 ctttttcaat gaaatgggag gcttttcggg cgatgatggc ttttttctcc acattctccg
    33901 cctgtcttac agtataacct gtctcatctt cccccgcttt tttaaaacgg tttatactga
    33961 ccgctccgcc gtgagtccgc accgcatatc ccttatcttc cagcagtatc aaatcacggc
    34021 gtatggtcat ttccgagaca tcggagaaaa tcttcttcag ctgcttgaga aaaacctctg
    34081 aatgatcatc cagcatccgc tttatctgta attgtctctg attcaactta acttacaccc
    34141 ctaatctgtt caatctgact atttcaccgt cggtaaagct cattgtcgaa acatgacctt
    34201 ttaacggaac aatcttctga tgtatggcat ctaatatcca gctcggttga gggaagaaag
    34261 acttctcata atcggctgcc ggtgtaatcc agtttctgga tcctacaact actgcaggtg
    34321 catccagata atcaaaacac agtgtggaaa ttgtcgttgc catatcttta aggtgagatc
    34381 ctctttcgca ggcatccgaa gcaagtataa ttcttccggt ctttttaacc gattgaacaa
    34441 ccgtgtcgta attaaacggt gaaacggttc ttgcatctat tacttcggct tccattccgt
    34501 atttttcttt cagtatatcg gctgcatcca aagccgtgta aagtgttgca cctattgtca
    34561 gtatggttat atccttaccc gctctcttta tatccggctc tcctatcggt acctcataat
    34621 attcagtagg tactccgcct tcatggaaca tctcgccttt gtcatacagt ctctggcttt
    34681 caaagaatat aaccgggtcg gttcccgata gagcggcatt tagcagacct ttagcatcgt
    34741 aaggtgttac cgggaatact accttcaacc ccgggatatg tgcacataaa ctggtccagt
    34801 cctgtgaatg ctgcgcaccg tatttggcgc ctacacttac tcttaccact accggcattt
    34861 tgagtattcc ggcactcatg gcctgccatt tgggaagctg gttgaatact tcatctcccg
    34921 ctctgcccaa gaaatcgcaa tacataagtt ccactaccgc tcttccgccc gccattgcat
    34981 atcctaccgc agagcctact attgcggctt cacttatgga ggagttgaac agtctgtgat
    35041 agggcatgga atcgcataat cctctgtatg cggcatatgc tcctccccat tctctgtttt
    35101 cctcaccgta tgcaaccata gtgggatctg tgtagaactt ctctatcatt gcttcgaata
    35161 tagcatctct tatctggaaa actttattct tggaaaccac cactccgtct acaatacccc
    35221 ttctctcttt cttctgaacc tgctgataac gggtattttc ttctaaaggt atgagagcat
    35281 caaccggtct gtcatccatt ttttctattc tttcattgga gaacataagg tcttcaatag
    35341 ctttgggatt acccacaaga tccatcttgg gggttatgga aggatctata gccattttat
    35401 atatcttgaa tatcagtgaa gccgcatcgc caagaattgc ttcaaactct tcctcttttg
    35461 ctattccgcc ttttacaagt ttctgcctgt attcgataag tgaatcctgt tccatccaca
    35521 tatctatttc ttcctttgtc ctgtatgccg aagcatcact cggagagtgt cctgcaaatc
    35581 tgtatgtcat gacatccaga agaaccggtc cttctttctt ttcaataatc tctctctttc
    35641 tcttatacgc atcaataacc gccaaaggat tgtatccgtc aattctctcg gcatgcatct
    35701 gctcggggtt tactcccgct ccgaaacggg caagaatatc aaagcccatg gtttcaccca
    35761 ttgtctgtcc gcccattccg taaaagttat tgaagaaatt gaatattatg ggcaatccgc
    35821 ctttatgctc gccttcccat aatgttttgt actgatccat tgtggcaaaa cagattcctt
    35881 cccataccgg tccgcaaccc aatgaagcat cacctaagtt tgctataacc ataccgctct
    35941 ttttgttaac cttcttgtaa agagccgctc ctacggctat atcggcactt ccgccaacta
    36001 ttgcattgtt ggggtatatg ccgaacggaa taaagaaggc atgcatggag ccgcccaatc
    36061 ctctgtggaa accggtctct ctggagaata tttccgccaa tgttccgtaa agcagaaagt
    36121 ctattgccag ttcttttacc gttttctggt ctttctgaac tatccttaaa attttgccgt
    36181 caaaatagct ctccattata tccataagct ctttatcgga aagtttctca atggccgaca
    36241 atccttttgc cagtatctca ccgtggcttc tgtgtgaacc taaaatgaaa tcgtctatac
    36301 ccagtgtata tgcctgtccc acaaccgatg cttcctgacc catggaaagg tgtgcaggtc
    36361 cgggatatgc atgctctacc gagttgtatt gtccggtagt tttgaccgca tttaacatac
    36421 tttcaaactc acgtattatt gccatatctc tgaagatacg taaaaaatcc gctttggaaa
    36481 atatcttctt ttcttcttca attgtcttgt tatatgcaca gacatctatg tccttgaatt
    36541 taatcttact tctttttctt aaatctttcg ggttcagtac cagttccttt gtcatttttt
    36601 aatctccttt tatattgtat ttcacattaa atctgcactt tgctatacat taaaatgcaa
    36661 acagcgcttc ccttataatc tcgcatacgg taggatgagg gaataccaat tccttcaaat
    36721 cttccaccgt catctgcgat tcaatcatca tggctgctcc gtaaatgagt tccgaagcat
    36781 agttgcctat gaggcttaca cctataagtc tgtttgtgct tttttctctc aatgctttac
    36841 atataccgtt tccgccttca ttttccgcaa cataccttcc ggaatatttc atggatatgg
    36901 taaccgcttc aaaatccata tttttctgcc tggctgaagc ttcggtctct ccgaccattg
    36961 ccacttcggg attggtatat attactgcag gtatggcggt atatctcatt atgtcttttt
    37021 taccggtaat gttatttaca catacttccg cttctctgta agcggtatgc gcaagcatgg
    37081 atatgccgtt tacatctcct gcggcataaa cttccgcaac attagtacat ccgaaacggt
    37141 cggtaataac ggcgccccgt ttatcggtct ccacccctat attctccagt cccaggtttt
    37201 tggtggaagg tcttctgcct atgcttatca gcactttttc acactcaatc tccacattct
    37261 ttccctcttt ttcgaatatg accgatttgt cggtaaagga cgttacttta ctttcaagat
    37321 taaacattat tccttttttc tcatattctt tacgtaaaag atccgatatc tcgtcgtcaa
    37381 tggggccgcc tatcttattg agcatctcca caaccgtaac cttgcttccc gcggaattga
    37441 aataggaagc catttccagc cctataactc ctcctcctac aacaacaaga gtttccggta
    37501 ttaccgtaag atccaaaatc tctctgttgg taagtacaaa ccccttttga tagccttcat
    37561 gaagtccggg aataggaggc attatgggtt ccgaaccgga gcatataagc agtctgtccg
    37621 cagtataaag ttcaccttcg gcacttacca caaaaccgtc agaagatttc ttttcaatct
    37681 ttgcaacctt ttctaccacc gtgactttgt ttcttttcaa agtccctttt actccggcta
    37741 ccaaagttct gaccacttta ttctttcttt taattaccgc ctcctggtcc aatgtggagt
    37801 cggtcacttt aattccgtac tgatcggaat gttttgtgta atcatatatc tttgcactgt
    37861 gaagtaaggc tttggaaggc acacatcctt catttaagca aactccgccg agataacgct
    37921 tttcaaacag cagtacattc agcccttttc ccgcagctct ttccgcacct aaatatccgg
    37981 ccggaccgcc ccctataact attaagtcgt atttcatatc cgcttccttt ctactttgct
    38041 cccatggttt caaaattctc aagattatta cataaatctt tcaagaatct tgccgcaggt
    38101 gctccgtcaa ctattctgtg atctattgtc agtgacagac ccattgcctg ataagtttta
    38161 atctgtccgt tttcttcctt aatgcgtgta gtaagtccgc agacacctaa aattgccgtc
    38221 tgaggaggat tgattatggg agtaaaggtt tccactccta aattacccaa attgcttatg
    38281 gtaaaggttc ctcctgtcaa taaatcgggg tttatgctgc cgcttctgca tttgcctgcc
    38341 aaatccctta cagcaacaga tatctcatta agcgataact tgtcgcagtt gaataatgta
    38401 ggaaccataa gacccctggg agtatcggta gcaacaccca tattgacaac attgaatctt
    38461 ttgattgttt ctccgttgaa atgagcattc aaatccttat gcaataataa tgtttttgct
    38521 gttgcatata ctattacatc attcaaagtt atatttgcca gccctaacct ttcgccgtat
    38581 gccttaacca tagccctgaa gttcattata cttgtggcat caaaagtagc attcattgtc
    38641 agctgagcca ttcccgataa cgaagaatac attgttctgg caataacctt ccttatatta
    38701 ctcaacttct cttcggtata gtcggcggta cttatttcat cggcagaaac agatattttt
    38761 cctttactta aatcttccac cgttactctt ccccctatac ccgttgcatc atattgagca
    38821 gccttgtcat aaccggtctt tgccgcggga gtaactttca ctccttcttt tacaagtgcc
    38881 tgtatgtctc tttctatcac tctgccgtcg ggaccggttg cggtagcata tgatacatcc
    38941 acatgggtct tctctgctaa cgctctggct ctgggagata tcttaagttg gtcggtaacc
    39001 gttgtctgta cccggacact ttccttctca accttaactt cttcttcagg cttttccacc
    39061 gattgctcaa ccggctgttc accgcccgga gcaaatgaag atacatcttc ccccggttcc
    39121 cctataacgc acatattcaa aaggcatgtg acatcgtcgc cctcttcaaa atatgtttct
    39181 aaaagtattc cttccacttt tgcttcttca tcaaaagtag ccttatccgt ttcataggta
    39241 aacaaaatat cgccgatatt taccttatcg ccttttttct tgtgccactt ggaaaccacg
    39301 caggattcta cggtatttcc ctgtctcggc ataataactg ctgttgccat ttatactcct
    39361 ccattcgttt aaatacaaga attgcaccat gttagattct aacatgccga tgttattttt
    39421 tcaacacggt ttgcattagt ttttatcaaa tatctgaatt aaaaaagcct gaactgagat
    39481 atacatacaa aaaaattata gattatatgc cttacgaata tccgtaaata ccttacttgc
    39541 ctgtttagta cgtccttcta aaatatccgc atatccggta aaatattctt tgtttgttcc
    39601 tttgtatcca ctaccgggta cctgctttct taaggtttag aatatctgat agtattttcc
    39661 actatttcaa atttatactt atcagaaaag taagggaaat tcaaataacc gtctgagacc
    39721 gtatattcta tatcctttac agtaaattca ccgtttttca tttcatatat cactttaaca
    39781 cttaatattg taagagacat tcctttttca gtcggctctt tttcacttaa acaaataaac
    39841 aaagccacgg ttcttccgtg ataccgttat tcacaatatc gcaggtcagc cattcgctat
    39901 aaaaaatatt gaaatcaggt ttaaccttat cggaagaaat ctttattaca atttgcccgt
    39961 tgttattctt atcctttaca gtaacaaaac atgcttgttt tttttctgaa tattcagcgt
    40021 tatactctat tgtatcggct ataacatcga aaaccgattc tgaagaaaag ggaatttctt
    40081 taaagtcctt ttcgttatat aaaccaatct gtcttgtttc tccatatgtt gtgattggat
    40141 caggtctcag caaaatttca ttatatccgt cagcattaaa atcttttaca acacagacta
    40201 ctgccggccc cgacaacata tgggtattaa gataaaatac ttcgttattt cttaatgctg
    40261 aatatattcg cttagcatca gctccgatta tccccggatt gagtgtatca gttgcatagg
    40321 atattcgtcc ggtgttttat gcattgtttc acatacataa acatcctgtt tttcaagatt
    40381 tgcaccgttt aagtaatata cattatcttt tgaaattttt ccgtttgtac cgtttattaa
    40441 tatgatatct tcaacattat cttcatttac atccttgtaa tccaagatat atgagccctt
    40501 ggtatttatg aacatacctt tattattttt agttattcta atcaattccg aattgttttt
    40561 gacaaactct ttttttgctt cacatacggt taacgataaa ttatcggtat aatttatttt
    40621 cttaacaatt atatcttcat atatctctac tgtaatatta ccgccttttt ttgatatatc
    40681 ttcaacaagt ctaaatgctc tattgtcctc tatattaact tccggtactt ctttttttct
    40741 cttcagataa gacaaaacaa tattttcatt ttcaaatgcg tttttttcca gacaagtttt
    40801 tatttccaac tttccgtctt cgcttaatct ttgatttatt tgctctatcg ataattcttt
    40861 tttactgtca tagtctaaaa aacatatttt ttccttgtat gtagtacatc tccgtttttt
    40921 cctgtaaata aattataagt tcatatttac cgtcaaaatc aatatcttct tttattacga
    40981 caggtatacc tttttcactt aattttatgt tttcaattac ttccgtcttt ttgccgtcat
    41041 attcaaaaca caaatttgta aagttagcta actcatcttt cccgtcataa tatattttga
    41101 aatcaaaccc cgttttatcg gatatatcta taacattcgg tgtttcgtca ggtaaaaccg
    41161 taataacggg tttcggtgtt tcttcatgtg ttttagtaca tgaagttaaa atcattacgg
    41221 taataaaaat gaatgtaaat aaaaataaat ttctttttct catctctcaa actccaattc
    41281 ttggtaaaaa attttttgtt ctaaagtgtt tactatgcta cattatgata gcattatttg
    41341 ttatttttgc caactatact ttactacgga ggcgtaccat gagagactac cttgagtgtc
    41401 gcatcttgag tcgtaccttg agtttccgta ctttttcacc tatcgattta aacatctgta
    41461 ttttctattg aaaatgagga atacttccgt tccaagaagc agatattcgg ctttgaaaag
    41521 ttcagagtga gaagtatggt gtcaattagg tctttgaatt ttatgataac agcctgcatg
    41581 actttccttg ctttaatgtc catgaagagt gtacgcagct cactactaat tgagatattg
    41641 cgtagagcag caccacttaa gcagaaggta caattctact attaccgtat agccaaaggt
    41701 atattaaaaa tactctctta tgcacgtaag ggtgtaagag ggtggttcaa agttaagcgt
    41761 caaaagtaca gacaattagc cttcagtttt ttatcctgag ctatacaaaa ttgaatactc
    41821 atgaaaagtt ccgacatagt gggaactgat tttcatacgc aaaactccgg ttttagagac
    41881 ttttatatgc aattttacta atctttcctc tatagtctta aagctacctc aaaaaccggt
    41941 caattattcg gtttcgattg cattttacgg aaactcaagg tgccgcgtat cgtccttatc
    42001 ccgccgggca atgtttatta tgcttttatg cgcataaaaa gacagaggac gactgctctt
    42061 tacatatatt tctctttttc atcctaaaaa cagactgctt ttataactgc ttcggcaatc
    42121 gccttccgca atcattattt taatgattcg ttattcacca ccgcttcttt aagagcgggc
    42181 atacttttca gaacggcaca atcgacggac ccgtcgataa tcgcggattc gagtaacaaa
    42241 tattccagtt tggtgaaaaa cttaagatcc tcaagactct ttacattatc ttgcttcagt
    42301 atgtgttcta tgcctttggc gccggttgca gcttgcacta tcacatagac acggtaatga
    42361 tcataaacat tgccctccac ttggtcgcga atctgaatcg acgtaattat ctgcatattg
    42421 tcatatgtaa actcaagatt ggccgaaaga aaagccggaa taagcagttt ttccaatgcc
    42481 ctgtccttaa aactctccgg caaaatgagt actttgacag aattatcttt agcatatttt
    42541 tcagcggcgc ttccggcttc ggtatatatt atcgctcctt cggggaatat gtccttttcg
    42601 atacatgtca cttcccctgg aatgaagaat tttgcaaacg actgcaaaca ggtcagcatg
    42661 tctttggtta tcgcagtggt cccgtcttcg aatttaactg tttccatgtt gccgtcgagt
    42721 ccataaaaaa tatcttttcc caatgatgtt gcaaacggaa gtttaacctt tttttgcgta
    42781 ccaacataat atccgccgcc tgtcatttca atcgtcattg aacgttttac cgttttatcg
    42841 gttgttgcga aagccctgtc cttgatgctt ttcagtttat cggatattat gatacggtct
    42901 acggtgctgt cttcaaacgc actttgacct atctcctcta tctgctccgt attacataca
    42961 atgatatgtc cgaacgcttt atagaatgcg taatcatcta tctttttcaa tgtggcgggc
    43021 aggtatatga cggcatcgaa gaaacctccg tcccctgcct tgtaaaatgt tgaagaaaga
    43081 cttttcacgc cttccggtat cactatatct ttactggtct gattacattt aatcagtacc
    43141 ccgtcgcctg cgacaacaaa ttcatccttg agactcttgt accattcagt atcatcaaag
    43201 gcaccgactc ctacgctctt tacgctctct ggtatgaata cttcttttac gtgcgaacat
    43261 gcggaaaaag ccttgtcttt gatacatgct acaggcttat tggaaattct gtcaggtatt
    43321 atcactgttt cttccgtgcc gtcatatttt atgattgtca cttcattgtc ttttacgatg
    43381 taaataaagg atccttccct acactctttt gcaggtttgc ttccgatatt gcaggccgtg
    43441 agcgacaaaa ctattaaaat cagcaatacc gccatgcttt ttttcatgtc atttgtttcc
    43501 tttccttttc atacttgagc gcaaatcatc gtatatacat ttcttggctg atatactccg
    43561 gggttactct gccagtcgcc tgttctgata catttcctaa cattattttc tttggtcact
    43621 atctgcgttg aatctccgcc gtctaccgaa atcccatgag aaatttgagt accccaaata
    43681 tttttaatca ttgctctaag ctcataaaga ctcagtccgg atgttatcgg tgacaaatcc
    43741 gagttggtac tgaataccga aagcaataca atatcttcat atgcattatt gagtttgaat
    43801 attgcacttc tgtgagttat tgaaagatgg actccatttg cttcgtctgc aactaaatcg
    43861 ttatattgtg tttctgacaa attttgtccc atgtataaat tcactccatc tatggcatat
    43921 tggatttgtg attgggttat ggattcgtta agatatgagt aattgttaaa acttgttaat
    43981 gaatccgcat aaatatatcg tcatgatgta tttctatagc ctcataataa acacttctac
    44041 ccgcatttgg tactgaatag aaatcccttc tataattagc catttaatac ctctccgttt
    44101 ctgccttacg agtatggcat attacgtttc atttgtcaac ggagtcgtga cacggagtcg
    44161 taccttgata ctttactaca gatatgaaaa tattagatgc agtgccctca ctcatagctt
    44221 cgtaccgcaa atcctactta cttatcttct caacatcttt ccacgcaccg gatactataa
    44281 gccgggtatg ctctttgaag atatagttgg ggttggaaag aggatgtatt ttaccgtcct
    44341 ctttgtatgc aagcacattg acgttaaagt tcattcttac attaacatgg gatattgtct
    44401 ttcctatcca ctgctcgggt gtttttatct ccgataccgc ataatcgggt gaaagttgta
    44461 tatagtcaaa catgtgattt gcactgaacc tgtatgccgc cctgtttgcc atatcctttt
    44521 ccggaaagat aatctcgtcg gctccgtttc ttaaaagaaa ttttgcatga atatcccttt
    44581 cggctttgga taccacatat ttagctccgt tatcctttag taatgatgtt atttccaaag
    44641 aagactggaa attctcccct atgcatacaa aacaaatatc aaagttattt atcccgaatg
    44701 attttaacac attctcatcc atgcaatccg ctaccaatgc ggaggtaaca tgctcggaaa
    44761 tctcttttaa ttcattttca tctttatcca ctaccattac ttcgtttccc agctccgcca
    44821 ttttcaaagc taagcattta ccgaattttc ctgttcctat tattaaaacc gatttcattt
    44881 tactcctaac ctattattat cctttcttcc atctgcctta tattcggcct gggtttatca
    44941 ccggccaaag ccgctataac ggtcaggctt cccaccctgc cgcagtacat taatattata
    45001 accgcaattt ttgataatat gttcaattcc ggggtaatac ccgttgtcag tcccaccgta
    45061 cttattgccg aaacagtctc aaacatagtg tcttcaaggg taaaaggctg tatcgacaca
    45121 ataaaaaagc atgccaaaga ggcaagtatt acatatatgg aggctatact gaaagctttt
    45181 ctcgggataa agtcttccag tcttttccct cccacattca aatccggctc gtgggttata
    45241 tatgctttta tggtaagtat gataacaaat accgtggtta tctttatacc gcctccggtg
    45301 gaaccgggat tggcacctat aatcatcaga agaatggtca acagtaaaga tccttccgaa
    45361 agttcggata tttccactgt attgaagcct gcggttctgg gagaaactac ctgaaatacc
    45421 gatgccaaaa ccttttctcc gaaagtcatg cctgctaagg tattattgct ttccaaaaag
    45481 aagaagaata ttgcgggaaa tactaccagc accgcggtac cgataagcac tatctttgta
    45541 tgcagtctgt atttggaaaa cttcaatttg ttatccataa tatcatccca cactataaat
    45601 ccgagaccgc ctatcaacat caacagtatt gcaaccacat tgacaagcac atcggcttca
    45661 aaacctatta atgaactcct tgaatcgaat acgcccataa gatcaaatcc ggcattacaa
    45721 aatgcggata tggcatggaa tatgccgtaa tatattccct ttaccgttcc caaaagcggt
    45781 atgaaacgga tggataataa agcggcggca atcatttcaa tggcaaagga tgtgatgata
    45841 atcctgcgca agagttttac aaccccgcct atatgcgggg tattcatggt ctccatcaga
    45901 agccctcttt ctctcaaacc tatctttttg cccaacatca gaaatatcat gcccgctaca
    45961 ctcatgaaac ccaggccgcc tgtctgtata agtaccaaga ttacaatctg gccgaacaat
    46021 gtaaactgag tatatgtatc gtatgtcgca agtcccgtga cacacagtgc agaggtggcg
    46081 gtaaacagag cattaataaa ggggattctt gtgttgtctt ttgaagcggc gggaagagaa
    46141 agcagcaacg ctccgattag tattattaca acaaatccta ttgcaataat ctgcatggta
    46201 ttcaatctta tcttcctttt agcttccatt agttataaaa gctccttaac catatttcct
    46261 attacctgtg ccatacgtaa aaaacctata tcattagggt gacaaccgtc taccgtacaa
    46321 ctgtccctgt aatctcttgc gaaaaaactt cttccgtcta taaaatacac atttctgtcg
    46381 ccgttttcca ttgcactcat atatgtctta aagattatat tccttctctg tatgctgtca
    46441 aatgagtaat cggaaaagtc gggtttggaa acaaatatta ccggaaggtc gggattctta
    46501 gctcttacag tgttaaaaaa cggttgatgt gttttttgta aatgctcaat cttcggagca
    46561 ttatggtcat aatccagaac aaatacgctc atatccaggc ttgctatata ttccgctatt
    46621 gcctgttctc cctcagctgc accggagaag cctaagttta tatagtcgac atcgcacatt
    46681 ctggatatga cggcttcata gcaattgccc gctcttgaag cacaaccgcc ctgtgtgata
    46741 gaggaaccgt aatacacaac aggcttcttg tgtttatatt gctttccggg acctaagaga
    46801 gaattttccg aaacccctat atatacctta tccacattat tgtataaagg aaaatacaga
    46861 gtaaagtatc gcagtttatg ctcactgaat cttattctca tctcatacca gtctttagtg
    46921 ttcacttcgg gattaaatct gctgaaaaaa acacttctgt tgtcaaaatc ttcatagata
    46981 tcaaatcccg aggcggcttg caaagacata ttggtcatcc tgaataattc gggaaagaag
    47041 acctgtatgc aaagataatc ggagtcggta caaaaacgta ttcttcctcc ggaggtatgt
    47101 aaatagtgta tacgcactct cgaattagac ttagcagcca cctcatcggg cattcttttg
    47161 aataccggtt gatttaaggg gtcatacaac ccgtatatat caaaaggttt gtttcttata
    47221 tccagatata tcaaatcctt gtcgtctaaa ttggtgtacg gctttaagcc tttgtaatac
    47281 gtttttgctt tttgcatatt tgttaatcct tctttctttt taattatact gcatattccc
    47341 gcttaccgtt gcaataactg ttgttccaag atttatttgt tctccttttt ttgccgttgc
    47401 ggtatttgtt ataacctgtg tattaatatt gtagttatcg gcaatccctt tggattgtgc
    47461 atcttttaaa gccgcttccg atgccgcttt ctttgcatat tccaccgctt catcaaaatc
    47521 atttgtcaca tacctgcctg aaggagcata taccatatat gaactgtcat actcgcttct
    47581 aatctctatt tgatataccg accttacatt gcttaccacc gctcctatcg cattggcata
    47641 ttttgcatcg gaaggtatta cacatcctgt gcccaaagcc tttgcgacat cgggaaggaa
    47701 tatatgtgta ggtgccccta taccgactaa agacatatct gttgcaaaac gaacaggcag
    47761 gtctttatgt aaacggtctt tatatgaatt gcgtataagg gccattgtct gctcatttac
    47821 cccgctttta aaatcatcgg gatatttatc ggttagcagt attcttacga tattttcgta
    47881 cagtttaagt tttacggtgt catataccat atcgcagaat tcttcctgag tatattcaat
    47941 acccgtttcc tcaaaaatgt ttgaaagaat atatttcacg gcggttattg aagcggtttc
    48001 gtcaaaggca ttaaaatctt tcttcacatg cattatatcg gtaggtgtta aacctgcccg
    48061 tattatcact ccctccgatt ccaatcgctc cgtgttaaat gtgtatatat ccttacccaa
    48121 agcatttgcg gctttttcca acggtaaggg ctgtttttcc aataaattta taagttccga
    48181 ttcttctttt gtatatttgt tgtgtatgat atgcgaatta ttaccggtta cacttttctg
    48241 taaaagtaaa aattcataca aaggataact gtgatacttt tttgaatcaa taagttcctt
    48301 aagttgctca attatacccg gccatcttgt tgcggcaaca cacagaggaa tcatgcgttg
    48361 aggcagttgc acaacctttc cgtgctccac ccttattgta ctgtctcccc ccaaaccgaa
    48421 tgtatctata aatacgccgt ggacaaatgt cttccacgag cctatcctta ccccggagtc
    48481 ggcattaagc ggtatgccgt tttttactat ggaaacgtcg gtagtggttc ctcccatatc
    48541 tataataagg caattttttt cctccgttat attcaccccg cccgtaaccg atgaagccgg
    48601 tccggacagg atggtttcca caggctttgt ttcggcatat tgttcactca ttttagtgct
    48661 gtcgcttcgt ataaccacaa cgggtgcatt aatgtgcatt ttttccaatg ccgactttat
    48721 actctctaag aactgtttga tgatgggtat aagtttggca ttgagtaaag tgcccgctcc
    48781 tctttgaatg gagtttagtt cgtaaaacag ttcatgaccg cataccgcag gaattccata
    48841 tctttcttcc accaacttct ttgcggtctt ttccagtttg gcaccgttac ttgcagcaaa
    48901 tacttccact attcccaagc cttcggcatc gttcaaccag tcatccagtt gagtaaataa
    48961 tttatcccag ttcggttgct gcgtgttgtt gccggaaaaa tcaccgcttt tttcacagag
    49021 atatacatca cttttgtcgg taagaccgta atccttgcct accatatcaa aaaccttttg
    49081 ctccactccg aaaaacagca acttggctct tccgcctttg ttttctatgc aggcgttggt
    49141 agccaaagtt gtggacagag ccactatatc cactttcgaa aagtattgct tatctaaacg
    49201 ggataacgca ttgattattc cggtctccag cttttcatga gtggtaagag cttttgcgga
    49261 gcataatact ttaccgctgt caaagtcata tattacggca tcggtatatg taccgcccgt
    49321 atctattcct attcctaaag ccatataact tttccgcctt cttttctcga ctgctctgcc
    49381 ttcattacat tgcgatgact gactatggtt gtatcgatga aggtaagatc gtcggtttcc
    49441 acgggttcac cggataacag ttttataaaa ttgttgacca gcgccatgtc acccgatata
    49501 tgtgcaaggc gtataactcc cgcatatacc gttttacttc cctttccgaa tctgttgatt
    49561 atgaatctgc ctttggattc gtcgcagtga atatcggctt tttccatgtg caggattgtg
    49621 tgtctgtaac atttttcgga aaatgccgta acattcaggt tgacatatct gttatcaccg
    49681 taatccattg tcaccacctg atggtccaat gcattgttgt cacacttata cacgcatctg
    49741 ccgaacagac cggtccgcaa cgaatcatat ttatcttttc tgttgtattt gggttttccg
    49801 gttatgacat ctcctaaaag tttcaagaaa gtaaatctcc atatgggtgc ggtaatgtat
    49861 aagctctcgg catcatacgg gcagttgttt ttaatatgct tgggacaatc caggcatatg
    49921 tcgccgcagc cttccggtgc attttctttt ttaaagtagg aaagactgcc gtaggaagat
    49981 atggatttgc aaccgtctcc catcaaccag tgtataaggt caaaatcatg gcagcattta
    50041 gccatcagca tgggtgtggt tttttccgaa tctctccagt tacctcttac ataactgtga
    50101 gcaaaatgga agtaacctat attttcggta tgctcgatag atattatatt tccgtacttc
    50161 ccgctcttta ccagttgatt catctttttg aagaacggcg aatatctcat tacatggcat
    50221 actacaacat atacaccttt ttcttttgct ttgttgcgta tctcttcaca ctcggcaata
    50281 tttatagaga cgggtttttc cagcattatg tgatatccgc attctattgc cgccatggca
    50341 tggcggtagt gatcgttatc cattgtgcat atatatatgg catcggccag tttcccggct
    50401 ttaaagaaat cgacttcatt cagatatctg ttacttttat ccacttcata cttgtcgccg
    50461 tatgtattga gtctgtactc atcggtatcg cataatgcgg ttattttagc ttttctgccg
    50521 tttaaaagtt tggtccatct catataaccg ctgcctctgt tgcctaagcc tatcacagct
    50581 atggtaatca tttgtcaccc tcccattctt tccatacacc cggttcatac cccgctgtcg
    50641 ccttattgcc gtttcgcacg acgggagttt ttatgaggtg ctgattttca aaaagcagcg
    50701 tcgtcctgtc ctgctctttt gcatatttaa tcctcagaag tttatcctga tcttttgttt
    50761 tatagtctaa aagattctcc attccgccta ccgctcttaa aactttttcc aactcgcctt
    50821 tactcatacc tttatcctgc atattaatat actggaaagg tatttttctt tctttgaagt
    50881 atcgttgggc tttttgggta tcaaagcatt tggtttttcc gaaaatctga atattcatta
    50941 ttccgcctct atatatccga ttgagtatat tttaacataa agcttgactg tataccgatt
    51001 ctttcgcttg taagtttacc gataggatac tataatgggc atgagagttt tttaagatac
    51061 ggaaaggaaa gttatgaagg cttttttcgt taaatacaga cggcttatat tacttatgtt
    51121 actctttctt ttgtatatat tttatttgat agtatgtgcg attgttaaaa aaagcgataa
    51181 cttcttttcc actgaaatgt ttcttgacag ctatattcct tttattgatt ttgccgttta
    51241 cggatatata tcctgggtgc cgcttgcagc cgtatccgtt attttccttt tcttctgtcc
    51301 gggcgacgga tattaccggc ttataatagc aattgcaata tcggttctta tttgcctgat
    51361 aataagtctt tcctatccgg tcagaatgga tattccccat ttcggcgaca gtgcatttct
    51421 tgttgcagtt ttgaaaaaca gcggtattgc aagttttccc aacatacctt catgtatatt
    51481 gtccaacggc tttttcttaa tgattttcta caaaaaaata cataaacgtt caaaatgctt
    51541 gccgatagtt ttgctgttcg gattgatatt gctggcatgg attgtctgtg ctttgctttc
    51601 caagatgaca catatttcag atctgttaat cggaatactt ataggagcgg tttcttcttt
    51661 gtcggtctgg tttattcctt ttaaacaaac acactaaaat ttaacttttt tcataactcc
    51721 gtcaaagaca taagaacaat cgtagcccgc tcttttaagc cggtctatcg ctttatcgta
    51781 agcaaacagt aacgccgatg taaaatggca gtcggaatta accgatacac gcaagttttt
    51841 cgcacgacat cttcttaaga tatattccga aggatacatg acatcaatgc cctttgccat
    51901 ggcaccggtg ttgacttcga tataggaatc ttttattaca tccaaagtcg cttctacatg
    51961 agctttatat tcatcggtat cggcatcaaa ccacttcttg cctatattcc ttttttccac
    52021 tatatcgaaa tgagcaatca ttaacgggtt atattctgtt atcatttgcc ttaatgtctg
    52081 atagtatttt ttgtaaaaag caaccccgtc accgccgaaa ccttcaatga tagctttatc
    52141 cactctttcc tgtgtaccgt cggccgaagc aaaaccgtct atccggtcgg aatagaggta
    52201 gtggaccgta cctataacaa aatccagatt aagttggtca aaatcaatat actttttgac
    52261 atccagccct tccaaatagt ccaactccaa tccgatataa atatttatct tgttcccgta
    52321 caccttcctc atgtaattta actcgtcaaa atacctgggc aggttctctt ttttcattgc
    52381 ccaatcatat ttcagcggca cgggagcatg gtcggaaaaa cctatacttt tcagtcccaa
    52441 tcttattgct tcttctatat attcatgtga ataatttatt ccgtcggaat aaggggtatg
    52501 taaatgtatt gtattaaatt tattcatcca tgttaacctt ctctcttata atatacaaag
    52561 gtctgttctt gctctcgtca aaaatcctgg caatatactc gccgtttata cccgacatga
    52621 taagctgtat gccgcccaca atcagtatgg caattacggc aggtatccat ccgttctgca
    52681 accgtattcc cataagcctt tgtatgatta caacgagaag ataaataaag cctgaagccg
    52741 ttgtgactaa acctatccat tttgtcagtt taagaggttt tgtggaaaac gacgttatac
    52801 cgtccatgga aagttttaac atctttttta aggggtattt ggtctctccc gcaaatcgct
    52861 cttctctttc gtaatatatt cctgtctgtt tgaatcccac ccatgaaaca agacctctta
    52921 catacctgtt tctctcggta agtcctttca atacatcgca tacctgcctg cttatgaggc
    52981 ggaagtcgcc ggtgtctacc ggtatgtcca cttcacacat gctttttaag aatctgtaaa
    53041 atactttggc ggtaaacttc ttgaagaagg tttcaccttt tcttttggaa cgtacggcat
    53101 ataccacatc atatccttct tcatactttt caatcatctg caacaccact tcgggaggat
    53161 cctgaaggtc ggcatccata actataacgg catcacctgc cgaatggtcc attccggcgg
    53221 ttatagcggt ctgatgcccg aaattacgtg aaaaagatat aagtttcaga tgagcatctt
    53281 cagcacaata ttgctttaca atagtttctg ttgcatcctt gcttccgtca ttaacgaaaa
    53341 taacttcata atcaaagtct ttatctttca ttacgtcggt tactcttttt atacattcat
    53401 gtattacctg ttcttcgtta tacagcggta ttacgagaga atatagtttt ttcatacaaa
    53461 acacctctcc aaatatgata ttcctattat acattttccc ggcttatatt aacagattta
    53521 ttgtaaggaa tataatatat aataaaatat taaatatttt gtattatttc ttatattatt
    53581 atgttatatt tttgtaatta tatattatat ttatattgac tattatatta aaaatgctat
    53641 aatttgacat atcataagca gaaaggggat atacggtatg gattcactgt ataaaaattt
    53701 tggtagtaga gtccgagaga aaagagtcat gaacaacatg tctcaagaaa aacttgcgga
    53761 gagatgtgat ttatcaacat catacatcgg cttggtggaa agaggcgaac gcaaggcgtc
    53821 tctggaagtt gtcgttaaaa tagcaaatgc tttggacgtt acacccaatg cactcgctat
    53881 ggattcgata aaacataagg catgcaacaa caaagacaaa ctggtattaa tgattaacga
    53941 ttttgatgat gcgcagttaa acaccacttt tgaaattgtt aatcagattc actcttacat
    54001 aaggaagggt acaaaaaaag caacttatat ggacatagac gagttgcttt ccgattagtc
    54061 cttatcttct ttcaaaacat ttttaacctt tttgaataag tcatttctgg tcaacataat
    54121 tttgtgaccg caaccgagac attccagtcg gaaatccgca ccggttctta gaatacgcca
    54181 tttgtttgag ccgcagggat gtgctttctt gagttccact atatcattta actgaatgtc
    54241 cgttttcatt attcaaaagt aaaagtgtcc agataacctc ttaaatactc cagatcggct
    54301 tcattgtgag cacctatcat tatggaaaga aagtatccgt tctttacaac agcgtacata
    54361 tcgaaaatca tgtacaggtc gtgtaaatcc aaagttaagg ttctgtttgc aaatttgcgg
    54421 ttacctaacc acacatttgt ctgattggat aaggttattt tagccccttc tatatttctg
    54481 tatgtatcgg ctttactgtt ggacatatat tcaatgtagg tatctttatc cgaataaccg
    54541 tatttgcttg catcttctac ggatatcatt atatgcgaat tgtcctgtgc atcatctacg
    54601 ggtttttgtg aaaagaaagc aagataggtt ataaagactt ccagttcatc gtcttctaca
    54661 tgctgcttta tatcaaattt agtatccagt tcctcttccg tagctgtgta ataattttcc
    54721 ggtaccttaa tcttaaatcc catatcttcg tttacataat acccgtcaac cacttcgcct
    54781 cgcgacacat gctccggttt aacaatctct atgtttttat tgcatcctac cagcatagta
    54841 gatagtatgg cgataattaa aatatatatt ataacttttt tcatattatt cccctattat
    54901 cttgacaagc actcttttgg gtcttttacc gtcaaactcg ccgtaaaaaa cctgctccca
    54961 cggacctaaa tcaagttttc cgtctgttac cgctattacc gcttctctgc ccattatagt
    55021 tcttttcaga tgtgcatcgg cattatcctc aaagccgttg tgggcatatt gcgaataagg
    55081 tttttccgga gctaacttct ccagccactt ttcaaaatcc tgatgcaatc ccctctcatc
    55141 atcgttaaca aataccgaag cggttatatg catggcatta cataaaagca atccttccct
    55201 gataccgctt tctttcagag attgctcaag ttgaggcgta atattcacaa accctcttct
    55261 tgtaccgata ttgaaatata gctcttttct gtaacttctc ataacactaa tatattttac
    55321 aaaaagaaat gtgaaaaaac aatattttca ggttgcttcc ccgccttttg ccgtttttca
    55381 aattattcat atggattttg cagtaaaaca ggaattattc gtaccatttt tgcaaaagac
    55441 ctttatttct ttcggatcct gctgttcatg caaagtaata agctctccgt cgctgcgctg
    55501 ttgcgtaaca ttttcaatac atacatcatg gaccacatcg ccgaaaaata ccgttgtctt
    55561 gaaaaacttg gaatggacat tgcttattct tactcccgat atatcgcccg gagtatatgt
    55621 ctccccgaag tatcccgtat aggagtagac aagctttaaa ttggttgcat ttggataaag
    55681 aggatgtatg aaaggcccgg tatgagtatc tataacatta tcaatactga cattcttaat
    55741 cttattgccg tgtcgtgata acaaagccac tccggacaca ccggaggtat atacatttct
    55801 caccgataca tcggtaatgt atcggttctc tatcgattcc tcatctttgc atatgttgga
    55861 cggaataaga ggaaagacat actgtttgaa aggataaacg agattaacat cggtggcgct
    55921 gttaatagca ataaggtcgt cggttgtttc cccggatata ttctctataa caacattgct
    55981 gcaccctgct cttatattca aaccgtctcc gttttttacc gtggaataaa accgcagatc
    56041 ggaaaaatgc aggttcttag agcggtctgc ggaaatcgtc caactcttca cttttttaaa
    56101 agtaatatca cgaatttcaa aattttcaca acgggaaata agcacctgga atccgcgcca
    56161 cccccagtaa tcccctatgg caagttgttc ttcattaaaa tggggatgcc aaaaacttgt
    56221 gtgcttcttt ggcccttcta tcagcgcatc attcttccct ataatcttaa tatttttagc
    56281 cgcctttata gataaaggat aaccgtaagg attatcggga tctgtctcca tattgtccgc
    56341 acggaataca ttgtcatgca caaaatcatt ctgcttaatc atcaccccgt cgattataac
    56401 ggtggtgtcg gaaggcaagg taattgctgc atcaatatag tagtcttttt tatcaaatat
    56461 tattgttctt tcatcacagc ttttagcaga gttcaggcaa tcttttatag cctgactgtc
    56521 gcttattccc tctttcaaaa aatccgaaac ataaaacaac ctgctcatca ttccgccgcc
    56581 cttatcttta taccgaatac cggtctaacc gattgtaata caaccggtta tcggtgtcaa
    56641 acaaacggca aaataaccgg taaatgcaag aaaaagaaaa actatgaaaa tttattaaaa
    56701 aggtattgtt cgtgacgtag cgtcatgaac tataatatac gcggaggtaa ataattatgg
    56761 caaaatacaa agcaattccg gacggatata tgaccatagg taaagcggcc aagaaaatgg
    56821 gtgtaacggt ccgcacactg cagtattacg ataaagaagg actgctgtcg ccttcgggcg
    56881 aaagtgaagg cggtcgcagg ctctataccg acaaagacct ggttgtgctt catcagattg
    56941 tatctttaaa atcattgggt ttttctttaa atgatataaa gcagcggttg atatctctgg
    57001 atacaccggc tgatgtggca aatgccctta ccgaacaggc agacaaaata cgcagtgaaa
    57061 tagaaaacct gactgcttct cttaatgcca tagaacagtt aaaaaccgaa gtattgcaaa
    57121 tgcagaaagt ggattttaag aaatatgccg atataatcgt taaccttcag atgaataata
    57181 aatattactt tcttattaaa aaatttgatg atgatacact ggatcatatc cgtgaccgtt
    57241 ttgatagaga gagcagtatg gattttataa acagatttga tgccttaagt gacgaaatac
    57301 tgaaacttca aaaagagaat gtaccgcagg aaagcgagca atgtcagcaa ctgacaaagg
    57361 cttactggga tttgattatg gagtttacaa acggtgacat gagtatgtta cagaagttac
    57421 cggaaatagc caattttgaa aacacaaata ccgactgggc taaaaagcag gctgttgtca
    57481 acagttattt agagcctgct tttgtcattt atttcaaaaa gctcggaatt aataatgtaa
    57541 gagaggagca acaatgaact gtgcaataca ggtaagtaat ttaagaaaaa gctacggcag
    57601 caataccgtt ctcaaaggta tcaatctgca tataaaagag ggagagattt ttgctctttt
    57661 aggagtgaac ggcgcgggaa agaccacagc tcttgaatgt atagaaggat tgaaaaagta
    57721 tgaaagcggt gaaatttccg taaacggaga aatgggtatt cagttacaat cctcctcttt
    57781 gcctgcacag ataaaatcca tggaagcagt caaactgttt tccaaatgga agaaagcaaa
    57841 tatagaccat gaaatgctta ccgccttagg catagaagag attaagaaaa agaagtatat
    57901 agagttatcg accggacaaa aaaggcggct gcatcttgct cttgcactta taggcaatcc
    57961 cgacatcata ttcttagacg agcccactgc gggactggat gttgaaggaa gactgtcact
    58021 gcatgaacag gtacgaaaac ttaaatcaca cggaaaaacc attgttcttg caagccatga
    58081 tatggcggaa gtggaggcgt tgtgtgatcg catagccatt ctgcataacg gtaatattgt
    58141 tttttgcggc accgctgcgg aactgaccga aaaactggga agaaaatatc tggtacatat
    58201 aaagacgcaa caaggggatc agacttttgc tacggacaac atagaagata cattattgtc
    58261 cctactcggt gaactgaaac aaaaaggatt atatttatcg gacataaaag tggacagagg
    58321 gacactggaa caacatttta ttgaaatgac aagaggtgaa gaagaatgaa gggtttttta
    58381 tacggtctta cattacaatg caaattggat ttacggagta aaacaatatt ggtaacctgc
    58441 tatattgtcc ctcttctttt cttcctttta atgggcagta ttttcggttc ggttatgccg
    58501 gaaatgaaag aaacactcat tccgtcaatg attgtgatga gtgtgtcaat gggagcattt
    58561 ataggattgc cgccttcctt gattgaaact tacggaaccg atgttaaaaa agtgtataaa
    58621 gcaaacggaa tccctctgca tgtcggcctt ataaccatgt ttttatccac attctttcat
    58681 ctgatgattt cctgcgtgat aatactactg cttgccccgg tactgtttga tgcggcatca
    58741 ccgaaaaaca ttcctctgtt tcttcttgca cttgcaatat atgctattgt ttctcttagc
    58801 ataggaagca tattgggact ggttgtcaag agtcaggcaa aggtaaccat gattgcacaa
    58861 ctgctgtttt tgccttccat tatgctttcg ggaattatgc ttcctattga aatattgccc
    58921 gaatcactga aaatattagg gcgtattttt cccgccacat ggggataccg actgatgttg
    58981 gataacggct ttcatatcaa aaacctgtgg tatctgataa ttgttttttg tattgcggtt
    59041 atagtatgta tgattcttct gaaaaagcag aaatttaaat gaccgtgtgc ttagcggaat
    59101 attatttatc cctttcaata ccgtactttt tgcgtatctc ttcaaaagca tattgtacgg
    59161 gaaccgtctc gcctctttga tattcaagga atccttcatt taacaggtta tcgatttctt
    59221 cttttgacat tttactaaaa tcctgcttca actctacctt tttcataagc attgttttcc
    59281 ttttctatat tgtactccca aaacatgttt tataccacac aaagcaaagg taccacacct
    59341 ttggcggcgg catggtaccc ttgttgttga gga
//
LOCUS       DAOSOM010000009        58687 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_572_length_58687_cov_6.105898, whole genome shotgun sequence.
ACCESSION   DAOSOM010000009 DAOSOM010000000
VERSION     DAOSOM010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 58687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 58687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58687
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_572_length_58687_cov_6.105898"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="PLF14_03595"
     CDS             <1..796
                     /locus_tag="PLF14_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT70415.1"
                     /translation="SEIYKKEEYEQSLTVSYKLHMELYIENLSKYYGEKLALDKFTYT
                     FKEGTYAILGPNGAGKSTLMHLITDNISRSGGQIFYDGKDILQLGRSFRKKIGFMPQS
                     QGMYDQMSAKGFLSYMAEIKGIPKKEARGVINELLSVVNLSDVSHKKLSSFSGGMRQR
                     ILLAQALLGKPDILMLDEPTAGLDPEERVRIRTYIGNLAASKIVLITTHITSDVESIA
                     DYVIFMKNGRIEAFGKPERILKETCSENMEEAYLKIIHTEGQNAGT"
     gene            783..3116
                     /locus_tag="PLF14_03600"
     CDS             783..3116
                     /locus_tag="PLF14_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70416.1"
                     /translation="MRELKRIIVSKTNIVCIAFLIFMNVFIFFWESTPKGDICTLPEY
                     TDRYIEVLNTCKGMDNEEGINYLNNLKKLSEGTVLIPRWLYSKDMEYKEEASGFYYKK
                     YGEQMIKDIESGKIDTSEQAVKKAYITIEVCNILIEKINHLTEYPEYIKTISENAENM
                     KSVSIFSDPNSFSYRNIIKTVEDFSKIHITELSLANDLAVNALFTDSISDYSILAYII
                     FIVLLMIQERKKGLWPLVYAYPEGRARLAIKRVAILLLCSVIATVFLVGSRFLTAAIV
                     YRGFGDMSRPIQSMSLFKGVSQPFTVGGFILNFFLFKIIGTWVISLILYFMMLAISNT
                     SLAVAVTSLFLGTEYTLFALIPDSYAIAVLRFINIFALINYNRIYMRYLNININEYPI
                     NGQTLTTYMIPVAMILFATGCIIIHHKKKPFSTPNPLLKLYDKILKLRAGLSDRFRLF
                     FAELHKTLWIQKGAFVIIVLLLWAIFASEAPPLNVSEYDGNLVYFENMFTGEVTEETL
                     TKIDEETVIAYEEMAKGSMHGATKVQYLSMIKAEALAKKGTGLWIMDSTIYDALLNRN
                     VEGYQRKTGLIAILFTTVMTAGIFAYEHQQKMKYLLCSTDRGRTVLVRNKTLIACVMT
                     CAVWLIVYGFEIRNAVKIYGSLHSLSAPVQSFMSEFPYKMTVLSFLILIYLLRLLVLL
                     SMTFMILLLSNLSNRANAAVITSLAVFSLPAAIVIMGGNIMEYFSVVKLLSPQENIPS
                     LILSYTLCFIPGIIALIANTVIWKGIRYENNNSKRFI"
     gene            3085..4215
                     /locus_tag="PLF14_03605"
     CDS             3085..4215
                     /locus_tag="PLF14_03605"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR00045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOT70417.1"
                     /translation="MKITIANDSYKGCMSAIACCDIMAKALNDAMPECSVNKYPMADG
                     GEGTTDCLVFATEGRKEYVTVNGVFGNKMTGFFGILGDNETAVIESACASGIQGIPKN
                     RLDPMRASSYGTGQLIRAALERGYKKIIVGFGGTAVSDGGMGTLAALGMVFYDKNKSI
                     LSPSGAAMTAVVSIDSSNVIKELKDAHIEFACDVENVYHGKEGAAYIFSPQKGATKEQ
                     VIELDAGLQNLAEVFKKQLNKDISNIPGAGAAGGLAGGLMTVCKPVIKSGFDVILSYT
                     DLEEAIKDSDIVITGEGHTDSQTVYGKLPMRVGMLSKKYNKPVICISGAISKDAEVLK
                     DYGITALFSISRGPISLKDSMRDSENLLYLKTFNIAGLLKLL"
     gene            4229..4305
                     /locus_tag="PLF14_03610"
     tRNA            4229..4305
                     /locus_tag="PLF14_03610"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4263..4265,aa:Val,seq:gac)
     gene            4458..4928
                     /locus_tag="PLF14_03615"
     CDS             4458..4928
                     /locus_tag="PLF14_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOT70418.1"
                     /translation="MTIKQMRQDKGLTQQKCADYLQIPLRTYKRYESDESKIKAIKHR
                     YIKERIAEYGFIDEEHGILTVEQIKEICKDVFASYDVEYCYLFGSYAKGKATEKSDVD
                     LLVSVQVKGIKFFELVENLRENLKKKVDLLDAAQLNNNPTLVQEILRDGVRIYG"
     gene            4995..5198
                     /locus_tag="PLF14_03620"
     CDS             4995..5198
                     /locus_tag="PLF14_03620"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOT70419.1"
                     /translation="MFRIIQIAENNSKLSDKFREIHNEIPWASIKGIRNIIVHAYGRI
                     NMTTVYDTVIYDIPNMYEKLKDI"
     gene            complement(5214..5879)
                     /locus_tag="PLF14_03625"
     CDS             complement(5214..5879)
                     /locus_tag="PLF14_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007070555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOT70420.1"
                     /translation="MKKDLQGKKRKNENKGNEASVWDRVAGIYDIFVYIINAKVHKAL
                     RKHIENLFEPSDIVLECACGTGMLTKSIAPKCQKLIATDFSKKMVQTTKYNCRNHKNI
                     SFAVADILNLEFADATFNKVVAANVIHLLEDPYKALDELDRVCKPNGKIIITSYVIKQ
                     EKKRNNMFTEAISKGTKYFKREFSFSSYKQFFIEAGYCDVQCIDIDGFVPCSIAIMGK
                     RGD"
     gene            complement(6053..7774)
                     /locus_tag="PLF14_03630"
     CDS             complement(6053..7774)
                     /locus_tag="PLF14_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT70421.1"
                     /translation="MKAFIKKSFALTDQGANDLIKSSTASFVTYIVNMLPAMLMMLFF
                     DKTLLLNERDNIFYYVFSIGVIVLLYILLSIEYSALFNTTYRESENLRIDIARNISDL
                     PLSYFSKHDLTDLAQTIMSDVATLEHALSHAIAKIIGFTGFFIIAAVMLLIGNYKLGL
                     CVISPIILYFTLLLISKKVQTKNFHKYYKKRRQNSDSFQEAIELQMEIQSFGMSDDVK
                     KKLYREVEEGEKIHIQSEFVGAAIISLASLILYLTHILVIIVGITLLGKGEINILYFL
                     GYLLAGIRMKEGAEANSFNMAELFSVDSAVKRINEIRNTKRQEGEELELSEFDIELKN
                     VSFSYDDNVQVLEDVSFVAKQNEVTALVGRSGCGKTSILRLVSRLYDVTGGEILISGE
                     NISNISTRSLFDKVSIVFQDVTLFDTSVLENIRIGKPEATDEEVKKAAALANCEEFVE
                     KLEDGYDTLIGENGANLSGGERQRLSIARAFLKDAPIIILDEIVASLDVDNEKKIQDS
                     LNKLTKDKTVLIISHRLKSIQNVDKIVVIDEGIVESQGKHEQLLKTSKVYKDLIDKTE
                     LAEKFVY"
     gene            complement(7758..9518)
                     /locus_tag="PLF14_03635"
     CDS             complement(7758..9518)
                     /locus_tag="PLF14_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT70422.1"
                     /translation="MGNVYKKLISYGKEKKHFLFLTIVFSVFAAFLQVLAFYHLYKLL
                     ENVILLDDMSNAKRYAFLIAGFLAGGGLLYFLSLILSHLFAFRIETNLRKFGIDGLTN
                     ASFKFFDLNSSGRIRKLIDDNAAQTHMAVAHLIPDNTGAMITPVLVLVLGFFISYRVG
                     IVLVVMTAVSLVLLKYMMGEQQFMKIYQASLEKLSSETVEYIRGMQVVKIFGVDVRSF
                     KALNTAIKEYAQNALNYSLSCKTPYVLFQELFFGVVAILIPVILLFTKAGSDLKLMSL
                     ELIMVFFLSGVLYTYIMKVMYLSMYTFQAQDAVSKLENMYKDMQEDVLQFGDKTEFKN
                     HNIEFENVSFGYKKDNLILENLSFKLEENKSYALVGASGSGKTTIAKLISGFYNVNDG
                     EIKIGGENITSYSKEALINEISFVFQDSKLFKTTIYENVALANKDASRKEVFEAMRLA
                     GVDGIIAKFKDRENTVIGSKGVYLSGGEKQRVAIARAILKDAKIVIFDEASAAIDPDN
                     EHELQKAFSNLMKEKTVIMIAHRLTSIRNVDEILVMENGKIIERGSDRELMKKDSVYK
                     HFQDTYKTANDWRVADESVH"
     gene            complement(9732..10649)
                     /locus_tag="PLF14_03640"
     CDS             complement(9732..10649)
                     /locus_tag="PLF14_03640"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT70423.1"
                     /translation="MSISNKIIMGSLWLTHFLNPPLKNTDEIYAHAKAHNKKQTFRVP
                     RNGRIEYSDLKIHTETDTYHCLRMKKKGQKPNKAILYICGGGGVYDYCRHQIFLARKL
                     LKRVDAEIYYPFYPPSTKYPVNEAYKMVFNTYRTMLNSYSNEKIGVVGVSFGGTAAIT
                     MISWNNYYKENLPMPTITVALSPGHVPADSTEKEMLEAYRGVDPFIPIEQVEAYGSIQ
                     KGKTEPEHWLVHTAHGDFRNAGKILVYYGKKESLAFAAPIYRQAFEKAQADYRIHIEP
                     DMPHCYGVVRINKATKRTYDEIVRFLNSL"
     gene            complement(10771..11502)
                     /locus_tag="PLF14_03645"
     CDS             complement(10771..11502)
                     /locus_tag="PLF14_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT70424.1"
                     /translation="MKSWIAFWNILRKDIKNYYLKPPNISWGIIFPLSWSLMQFIRSP
                     GADMSELLPGLIAMSVLFGTTSMLAVTITFERSGKSFERLLLAPISLSLLVLAKIAGT
                     ILFGIFNAFVPIILASFFIDLHAINWQVLLFAILMIAITSALTGLLVAVSAEQVFEAQ
                     TLSNFFRFPMLFLCGLFIPISSLPVFLRPVSFCLPLTYGTDILKSTITGDNMLSPLLS
                     FGILLIFAAILFFICRHIINRKWIL"
     gene            complement(11499..12467)
                     /locus_tag="PLF14_03650"
     CDS             complement(11499..12467)
                     /locus_tag="PLF14_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT70425.1"
                     /translation="MGIINVENLKKTYGNVVAVNDISFKIEKGEIFGFLGPNGAGKTS
                     TINMMTGLSRPTEGAITVADIDVVKQTKKAQAIMGIVPDESNLYNEMDGFSNLCFCAA
                     LYGMRKKEREGKAYQLLEQFRLNDTGGRPFKAYSKGMRRKLTIAAALIHSPQILFLDE
                     PTTGIDVESAGQIRDLIVRLKAEGTTVFLTTHYIEEAERICDRIAFIAKGRIIATGTV
                     RQLMESVSHGYAVRLVTDKCIGNLSVELQNRFKVSVITSQSDHSLVMQSEQRIPLFPV
                     LKFLHDRKIEVHEARELHPSLEDVFVKLTGIESCLMKKDKEKGDKR"
     gene            complement(12473..12868)
                     /locus_tag="PLF14_03655"
     CDS             complement(12473..12868)
                     /locus_tag="PLF14_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOT70426.1"
                     /translation="MDKKKILKAIADETRMSIVKLLLSHNYCVRALANELKLTEATIS
                     QHLKVLREAGLLVGEKRGYFMHYTVERAALRELAKEIDTLAMIQQGTCEPEIRKDCPA
                     DEKKECPKKGQCSETVREFCHGKSQGKEK"
     gene            13075..14532
                     /locus_tag="PLF14_03660"
     CDS             13075..14532
                     /locus_tag="PLF14_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HOT70427.1"
                     /translation="MFSVKTIVNDLLQYTSEREWFEFKENWYEPYEIGQYISALSNSA
                     VFEGRRYAYLVWGISDKTQEIVGTNFDYNSSVKNEPLKHYLSRQLSPFIDFSFDEGMI
                     QGKRVVLLRIPATKTVPLSFDNERYIRIGSSKEKLRRYPEKESNLFYYLRSGHPTIEN
                     TPSAYQDLTFEKLFIYYGAKGLKLNQDTFKKNLSLLTEDGKYNVLAQLLSDNSQLPLR
                     VAIFSGKSKTSELFSVKEFGYQCILYTLEDVLRYGDILNIIQADERGRVVERKDVPLF
                     EYEAFREAIINAFVHNKWVSGNEPMITVYSDRMEILSRGTIDPEQTMEGFFAGESVPV
                     NKKLSEIFLQLHISEKTGRGVPKIIDTYGRAAFEFRENSIVVTIPFRWINMPGDKVSD
                     KKTKQALPTRDRIFYAIKNNPDITIPEIATLLGVGKTTVDNGISVLKRNGKIERIGSN
                     KTGYWKIVNQFKKAAPLQNSFLVSEKESAYEKGED"
     gene            14623..14940
                     /locus_tag="PLF14_03665"
     CDS             14623..14940
                     /locus_tag="PLF14_03665"
                     /inference="COORDINATES: protein motif:HMM:NF026041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HOT70428.1"
                     /translation="MFNFLEMFEKSLGLKEPWKVTKAQFIEENRSVHVYVEADKNAEY
                     PCPECNKMCKRYDYEDKERTWRHADVMLYPCFVHCMMPTVKCEEHGINVTDAPWESIN
                     PLP"
     gene            15053..16114
                     /locus_tag="PLF14_03670"
     CDS             15053..16114
                     /locus_tag="PLF14_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70429.1"
                     /translation="MRLLYDKVYEQIRKTDFESLWKGFHAYHFALYNSDSVYFKDKTG
                     DYEECFLGNTSIKYGDEQIAIWRVDDYTKEDPVLLAANIIHEMFHAYQYECREKRFPD
                     DLKALDYPIVSDNILLKYAENQQIVKAVKSNDRRIIKECLSSVFASRARRKELIKEYI
                     DYEYKMETVEGMAEYIGLCALKQLDYEKYENKINKYIDNLTDTDNILFDNRRLAYYSG
                     CLLLIALKQADICFFHDISKETKTVYELVAEGFPAADLPEILHKDAIEESLKRYIDNR
                     KKLFSDFFSKNPECTEKSFKICGYDPMNMVKLDNLVWCKYFVMLKEKAKEDPLLIYNQ
                     VVLKVDETDTTSVSAYYTV"
     gene            complement(16118..17152)
                     /locus_tag="PLF14_03675"
     CDS             complement(16118..17152)
                     /locus_tag="PLF14_03675"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOT70430.1"
                     /translation="MKEVYAVTGALGFLGNAIVDNLLSKGKTVRVMDIGQDKYGLLKD
                     RDVQFFRADVCDAASMEDFFTVEDDQELIVIHAAGIVSISSKFSQLMYDVNVKGTQNI
                     VDMALKHKIKKLVYVSTVHAIPELPKDEMIAEISDFNPDLVEGEYSKTKAIATKIVMD
                     SVQKGLYAMVVHPSGIIGPGDYAGGHMTQLVKDCAEGRLTACVKGGYDFVDVRDVAEG
                     IVLAAKKGKSGNAYILSGEYYPIKKITDTICSVLGKKQIKTILPLWFAKMTAPLAELY
                     YKIKKQKPLFTSYSLYTLQSNSNFNSKKAQQELGYKLKFSLKQSIEDTYMWIKANNLI
                     RRKKKVASKA"
     gene            17278..18027
                     /locus_tag="PLF14_03680"
     CDS             17278..18027
                     /locus_tag="PLF14_03680"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOT70431.1"
                     /translation="MDNPFISHLRKPPKLKGIKKALFIQPHADDNQIGAGGMMALLAD
                     SGAEVYELTVTDDRRLEQDKEGLTTRQSEAVAAQQYLNVKNAGFLGFCDKTQEPVEKI
                     ALKIMEVIRKIKPDAVFTVDPDLSTECHSDHIKTGMAVKYAVMDAACDFYPYSEDGKA
                     RHETWTTPILAFYYTDKPNRKADISKVMDKKINSIKCHKSQVDSNLLMFVKELAKIAA
                     KGTKFKYAEQFRALSSLQMHCFNIALEEDND"
     gene            complement(18322..20166)
                     /locus_tag="PLF14_03685"
     CDS             complement(18322..20166)
                     /locus_tag="PLF14_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT70432.1"
                     /translation="MEEDNREIFNKNIDLKMWKKAIKYLAKYMNHVIGIVIAMIINAA
                     TDAALPLFTEYAIDNFIAYKTTEGLGLFIILYVLLLILKGMTIYTFIYLADKLWHLVS
                     HDIRVDGFKKLQELSFSYYDRNATGSLFTRVTSDVGRLTGIISWGLVDMIWAISMIIM
                     MTVYMFMKNVKITLVVLTVIPILAVISVFFQKRILFYSREARRYNSIITGAFSEGIQG
                     ATTSKTLVREESHISDFEKKTDDMRTASIKTAVYSGLFFPIVSVLGIIGTSLAIWQGG
                     VMILGEVITVGTLTLFISYTLQFFHPVNDMARIFARFQFSQAAGERIMALLEADIDIK
                     DSDEVIEKYGNITSDMPVPKLKGSIRFENVSFRYKEGEKVLDTFNLSVEPGQCIALVG
                     ETGSGKTTIVNLTCRFYEPTEGTIYIDGQDYKTIPLMYIHKNLGYMLQAPHLFSGTIM
                     DNIRYGREDATEEEVINAAKMVEAHDFIMKFENGYNHVISKGGGGISTGQKQLICIAR
                     AIVNDPCIFVLDEATSSVDTQTEKAVQRAISKVLHNRTSFVVAHRLSTIREADRILVI
                     DKGKVIEQGSHEELMKLKGHYHNLYTSQFIDDSNKDLFGTPEPLPVED"
     gene            complement(20172..21968)
                     /locus_tag="PLF14_03690"
     CDS             complement(20172..21968)
                     /locus_tag="PLF14_03690"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT70433.1"
                     /translation="MKKYRLLKQFLRGNKAAYIAGIVFMIIAQLLSLVNTFIIGVVID
                     SVIGDILPEGIAYKGLQLLGGKEHLMHNLWICGLVLVFFSAFEGVFMFLSRKYSAIAG
                     EGIAKNIRMDLYEHLQNSTYLYNTSIKTGDIMQRCTSDLETVRNFLATQFQDLIKCIV
                     LIVASVSVMQKVNTDLTLAGFSLMPIIVLGAFFFFRYVSKKFLLADEAEGVMSTVIEE
                     NLSGMRIVRSCATQSREIEKFIASSLDYRNKDLKITVAFAWYWGLSDAICQLQIGVVL
                     VTGIILAVKGEITVGMLTVFMTYVHNLIWPIRGMGRVLGDMGKASVSLGRIQEVLEAP
                     REPGRDDTGLTPKIEGNISFENVSYEYTDGNEVLKDISFSIGKGETLAILGKTGSGKS
                     TLLHLLVGLLPYEKGSIKVDGIELGEINKRHLRKNIGIVLQEPFLFSKTIKENISDNN
                     PDITDADIEKATSIASVDRAIRNFEKGYDTIVGEKGVTLSGGQRQRVAIARTLIGNCK
                     VLIFDDSLSAVDMQTDKEIRNALRKHCADTTTILISHRINTLRQADLILVMEDGRITD
                     RGTHDELIQREGLYKQVYDMQSCVLDMAKAGE"
     gene            complement(22257..22853)
                     /gene="recR"
                     /locus_tag="PLF14_03695"
     CDS             complement(22257..22853)
                     /gene="recR"
                     /locus_tag="PLF14_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOT70434.1"
                     /translation="MADIYPKPLSRLIAAFEMFPGIGRKMATRLAFHLLTMDDERIQY
                     ISESIRDAKAGLTYCSVCCNITQKDPCEICSSSRDHSVICVMSSVKDVMAMERTCEYD
                     GVYHVLHGIISPMEGVNPEDLKIKELLKRLDGNVKEVIIATNSSVEGEATAMYLSRLI
                     KPFNIKVTRIAHGISVGTEIEYADEATLIRALKGRTAI"
     gene            complement(22854..23186)
                     /locus_tag="PLF14_03700"
     CDS             complement(22854..23186)
                     /locus_tag="PLF14_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOT70435.1"
                     /translation="MARGRMGGGNMNYSSMLKQAQKMQADIAKAQEELENSTFESKAG
                     GGVVTVAMKGDYTMDSITIDPVVLEDADVEMLQDLILTAVNESVGKINAKKEETMGKA
                     TGGMRMPF"
     gene            complement(23201..24721)
                     /gene="dnaX"
                     /locus_tag="PLF14_03705"
     CDS             complement(23201..24721)
                     /gene="dnaX"
                     /locus_tag="PLF14_03705"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOT70436.1"
                     /translation="MSHKALYREWRPSSFSEVVEQEHITKTLKRAVITGRIAHAYLFS
                     GTRGTGKTTLAKIFARAVNCLHPNEGDPCNKCDICTKASEEVLVDIAEIDAASNNSVD
                     NIRRICDEVNYMPALAKYKVYIIDEVHMLSTSAFNALLKTLEEPPAHVIFILCTTEPD
                     KLPATVLSRCQRFDFHRITTKGISNRLRKIADAEKQQITDEALDMIARISFGALRDAI
                     SLLDQCISSTDDTIDVTDVLNCAGLIDDMSNAKVVQALVDNDVTGILNSVNEVIAEGK
                     SPAGFLSGLLGYLRNILVCMNVKEPDRFIEADGNAVSEMKRLSKLMEPEEVIWYIKEL
                     SENEAVLKRTSRPKTYLEIMLLGLLVKNTEKVQPKVQVKDKPVRKEVQKADAVKPAVN
                     ETSKPVKTETKSNQNISWSKYIQDRFDKGSKDVFELLQSTIEISGNQAVVTVANDFLK
                     SSLERNGVAVLEEIRGYSKDITNARFVVAGTDKVAGSPEQQITEIGKKHNIPVEII"
     gene            complement(24745..25009)
                     /gene="ffs"
                     /locus_tag="PLF14_03710"
     ncRNA           complement(24745..25009)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLF14_03710"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            25254..26630
                     /locus_tag="PLF14_03715"
     CDS             25254..26630
                     /locus_tag="PLF14_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006447798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HOT70437.1"
                     /translation="MSTNDLMDFVSKNVNISSNPSELKITDMRFVDIDGAPKRNTLIK
                     IMTNQGIVGYGEIRDGSSKTYALMLKSRILGENPCNVDRIFRKIKQFGGHSRQGGGVS
                     GIEIALWDLAGKAYGVPLYQMLGGKFRDKIRMYCDTDVEGRHTGKDMGNALKKRMEMG
                     FTFLKMDLGIGLLLDIPGTLNAPLGFIEDMRKYSSHILNVQGGSVTKEMVKHRKSYDI
                     VNTAHPFTGIHLTEKGLDYLEQYIKDVRSVIGYEVPLAVDHFGHVCVEDCIKFARRME
                     PYNIAWIEDMIPWIYTDQYVRLKNSTTIPICTGEDIYLKEGFAELIKAGAVSIIHPDI
                     LTCGGALELKKIADIADECGVAVAVHMAESPIACMAAAHVCAAMRNVLAMEFHSVDVP
                     WWSDIVVGLPKPIIQDGYCTVPDKPGLGIDALNEEVIKEHINPQIPGMWEDTSKWDKE
                     FSNDRIWS"
     gene            26675..27577
                     /locus_tag="PLF14_03720"
     CDS             26675..27577
                     /locus_tag="PLF14_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HOT70438.1"
                     /translation="MHFNSKEDIIEMTPLWKGERLEDGRPYVDPKYLTELRKMTLEEV
                     WKPIYVKGYENQFEGRLKTLHDDGRKLIGRAVTCTFCPTRPDLHEIMFRIGSNEGRKG
                     NYNQWVIDSLVEGDVVVADMYDKVYKGTFLGGNLTTAIKNRTKTGGGVIWGGIRDVEQ
                     MKQAEGIQVYYRGIDPTPIRECVMTGFNTPTRIGNAICLPGDIVFGAGGGVLFIPSHL
                     VAEVVEGAAKTHIKDEFGFEMIKQNRFTTAQIDRNTWTVEMLDMLVDFIQKDERGKNY
                     RDIDWSQEYYMAKHGDPNDTQTAL"
     gene            27587..28279
                     /locus_tag="PLF14_03725"
     CDS             27587..28279
                     /locus_tag="PLF14_03725"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT70439.1"
                     /translation="MKLRWIGQSGYILEDNTTEIIIDPYLSDIVNKVANRPRLVPPPI
                     QPEDIYPDYVICTHDHLDHIDPETIERMNKDTVCFIAPSSCKERLSALGCKNIKTIDA
                     GEQISAGKFQITAVFAYHSVDAIGIIAEQDSIKLYFSGDTLYHEKLEELKESGITVMF
                     VCINGKLGNMNADEAAKLTKIINPLVGVPTHYGMFASNTEDPEKYTSQIDNGFIMDFN
                     KEYTISELLRGE"
     gene            28282..29025
                     /locus_tag="PLF14_03730"
     CDS             28282..29025
                     /locus_tag="PLF14_03730"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009284561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT70440.1"
                     /translation="MQHKLSGKRALITGSGRGIGRAIAIELAKNGADIIVHYLGNVKS
                     AEQVKKQIEALGGSCSIIQADLCKPETLEGFIRKIGDVDILVLNASVQYRNQWENISL
                     EEFYDQINCNLLASMLLIQQAVPYMRKKHWGRIITIGSVQEAKPHPDMLVYSASKAAQ
                     TNMVKSLAVQLAKDGITVNNVAPGAINTDRNKDVLEDKEYYKKVTDSIPAGFIGEPAD
                     CAGIVSLLCSEEGRYITGQSIYVDGGKCL"
     gene            29043..30347
                     /locus_tag="PLF14_03735"
     CDS             29043..30347
                     /locus_tag="PLF14_03735"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HOT70441.1"
                     /translation="MSHTLLKFFEEQKMQSNKRIEGDIQKYRKAFAKIKVVNSQGELI
                     ENVKGRIKQKTHDFKFGCNIFMLDQFPDEEHNKAYRKEFKKLFNYTVAPFYWQDFELK
                     DGEPRLGKNPVNVYRRPSIEKILKYCGENNIDVKGHPLFWHLFLPQWLVQDKNESYFR
                     IERRLREIAEVFKDRIRDWDVVNESLSRPPHRENYQLPYDYVNRIFDLADRYFPKNRL
                     FINDTLRFCWSEFIWELSPYYLQIENMLMKGKRIDAIGFQYHLFKTPERLEREAEILL
                     NPTCLFNVMDTYGKFHKPMHVSEVTVPAFGLTDENKEVQAKLTEWLYKVWFSHPDMES
                     IVWWNLVDGTAAYAPLGSAEGENYYGGGLLNYDMSKKPVYHAINNLINKEWHTDLKFN
                     KNECLFNGFFGEYDIEVIADDKITHHKIHLSKDGSREFVLTV"
     gene            complement(30306..30482)
                     /locus_tag="PLF14_03740"
     CDS             complement(30306..30482)
                     /locus_tag="PLF14_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70442.1"
                     /translation="MKFKIIFSENAKKDLISIVRYISEELLEPVTAEKLSQRILKAIK
                     PSDSQYKFSASVFT"
     gene            complement(30479..30784)
                     /locus_tag="PLF14_03745"
     CDS             complement(30479..30784)
                     /locus_tag="PLF14_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009219267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HOT70443.1"
                     /translation="MARTSNIYVRVEPYIKEQAEEILEKLGISMSNAVSIFLRQVVMH
                     NGLPFDVKIPDSKPLSLPILSEAEFNMEMLKAHNDFENGRIYGLEEVEEEFKKELGK"
     gene            complement(30857..31573)
                     /locus_tag="PLF14_03750"
     CDS             complement(30857..31573)
                     /locus_tag="PLF14_03750"
                     /inference="COORDINATES: protein motif:HMM:NF027552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4886 domain-containing protein"
                     /protein_id="HOT70444.1"
                     /translation="MKILTIGNSFSQDATVYMEKIALSDIYVRNLFIPGCDLKMHCDN
                     IKTNEKAYEYQKNGKKLCMISLEKALNKEQWDVITIQQVSHLSGILNSYFPYISELIN
                     YIRQNVPDAKIMLHKTWPYAHGCQHSYFSDYNNNTKTMYDSINRTVKIISAQFNILVI
                     NTGDCIYNSYTECLFNPDYEGISLFRDGFHLSIPHGRYLAALCFYLHFYNKDQINLKY
                     LPKGMTYKQRDCLQSLAMKH"
     gene            complement(31551..31841)
                     /locus_tag="PLF14_03755"
     CDS             complement(31551..31841)
                     /locus_tag="PLF14_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70445.1"
                     /translation="MAQILEAAMIICFGISWPVNIAKSLKSKTAKGKSIVFLFFILIG
                     YLCAIAGKIISDKINWVFWFYVVNTLMVSLDIIIYFNNRKRDKEKDENTHNR"
     gene            complement(31843..32859)
                     /locus_tag="PLF14_03760"
     CDS             complement(31843..32859)
                     /locus_tag="PLF14_03760"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008751963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOT70446.1"
                     /translation="MNKINTKLMDVPDEVSLKYCADIIKRGGTVAFPTETVYGLGADG
                     LNSSAVEKIFIAKGRPNDNPLILHIHDISVLDKYVKEVPKTLTVLSRLYMPGPLTVVL
                     KKRSIVPDSVTANLDTVAIRVPDNEIALKLIEYSDTAIAAPSANISGKPSPTMFEHVV
                     ADMSGKVDAIINGGKCRIGVESTVLDLTSDRPVILRPGGVPYDSLKLVIKDLETAYAL
                     NTDTPKSPGMKYKHYAPKADMVIVKGETHKVLEAVNKMSGDDYGVLCCKETFDRYNTH
                     NKLCAGSINNPSEIASNIFECLRLFDMMRIKKIYCEYFDTKDIGEAVMNRLLKAASQN
                     VIEV"
     gene            complement(32846..33925)
                     /gene="prfA"
                     /locus_tag="PLF14_03765"
     CDS             complement(32846..33925)
                     /gene="prfA"
                     /locus_tag="PLF14_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOT70447.1"
                     /translation="MLGKLKEIEIKYEDISRQLTDPAVISDQERYVALMKELNGISEI
                     VKVYREYVQTAKEIDDIYLMLKENSDDADFKQLLQEELAQLEEKKPALEQQLKILLLP
                     VDPNDTKNVIIEIRGGAGGDEAALFAADLLRMYTMYAEAKKWKTEILDLNEIGIGGIK
                     EVVFSITGQGAYSRFKYESGVHRVQRVPETESQGRIHTSTVTVAVLPEAEEVDVEINM
                     NDVQIDTYRSSGAGGQHVNKTDSAIRLTHIPTGIVVACQDERSQHKNRDKAFKILRSK
                     LYEIAQENQDKEIAENRKSQVGTGDRSERIRTYNFPQGRVTDHRIGLTLYKIEAVMNG
                     DLDELMDALITEDQARKLLSQNEQD"
     gene            complement(34073..35191)
                     /gene="wecB"
                     /locus_tag="PLF14_03770"
     CDS             complement(34073..35191)
                     /gene="wecB"
                     /locus_tag="PLF14_03770"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004443538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOT70448.1"
                     /translation="MIKIMSVFGTRPDTIKMAPLVNLLDKEKEIDHIVCVSGQHREMV
                     NEILDIFKITPKYDLDIMIERQTLEHVTSSILMKLSDILAKEQPDMILVHGDTNTCLS
                     ASLAAFYKKIPIGHVEAGLRTDDIYSPYPEEFVRRTVDSISSLYFCPTINNADNLIKE
                     NISKDGIYITGNTVIDTLQTTVKKDYTFKEPLLNRIDYNNKRIIPITAHRRENIGVNL
                     ENICNAIKTLASKYPDAHFIYTVHPNPAVKNTANRILEGIDNVSLLPPLIFTDLHNLI
                     ARAYFVMTDSGGLQEEAPSLGVPVLVLRRETERPEAVQAGTVKLTGVEYKNIVRDGRM
                     LFDNKSVYDKMSNAVNPYGDGKASRRIVDAIKEYFKNL"
     gene            complement(35184..36293)
                     /locus_tag="PLF14_03775"
     CDS             complement(35184..36293)
                     /locus_tag="PLF14_03775"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOT70449.1"
                     /translation="MSYIYTFLASFIIAFGLCPLVIKFAKAKKILKYPRRNRDVHTEP
                     VPLLGGIAIIISFGLSVFGNYLFNPNFIVTYEVIGLAVGIVIISVMGILDDIYDLKPV
                     IKVVFQVAAAVATVAISGSKIAYFTNLGTNNNLVHLPDIISFIATVAWIFMLTNAFNI
                     IDGLDGLTAGTTVICSLTLFFVTFVRPDAGLVGEYIPILLIALSGAAAGFLPFNFNPA
                     KLYLGETGAAFTGFCIAVLSVQGTMKAHAAIAVFIPLVAMGLPVLDISLAYFRRIIMK
                     KPIAEGDKDHIHHRLMKMGLSQRKTVLILYLIDIVFGITAFLLSGQQYSRFWLYFAII
                     IILMGLSVYILYKPLLSQAAKNGNTEVMEEEQSND"
     gene            complement(36295..36741)
                     /locus_tag="PLF14_03780"
     CDS             complement(36295..36741)
                     /locus_tag="PLF14_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HOT70450.1"
                     /translation="MERPSWDEYFMQVVHLVKTRSTCLRRAVGAVIVKDKRILATGYN
                     GAPVGCKHCEETGCVRMQMNVPSGEKHELCRAIHAEQNAIVQAARAGISIDGSTIYVS
                     IQPCVICAKLLINAGIKRIVFEGDYPDALSKELLEEAGIELVKYGG"
     gene            complement(36741..37829)
                     /gene="upp"
                     /locus_tag="PLF14_03785"
     CDS             complement(36741..37829)
                     /gene="upp"
                     /locus_tag="PLF14_03785"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOT70451.1"
                     /translation="MLAIGCDHAGFKLKSEILLYLEEKNIPYRDFGSYSEDSCHFPFY
                     ASLVASAVQKNECERGILICGTGIGMSIAANKFKGIRAALCMDEYQASMAKRHNNANI
                     LVLAGRITPKDMACKIIDIWLNENYLEGKYELRNGLIRRIEEGGSMSGTNYMDMAFVF
                     DHPLIQHKISIMRDKNTNTKEFRELVTEVSTLMGYEVTRELPLEQVEVETPIAKTTVK
                     VLSGKSVGFVPILRAGLGMVDGLLTLIPTAKIGHIGLYRDHETLQPVEYYCKLPEDAH
                     ERMIILLDPMLATGGSASAAIGFLKQRGVKNIKLMCIIAAPEGIKKVHDDHPDVNIYC
                     ACVDKALNDDAYIVPGLGDAGDRLFGTK"
     gene            complement(37966..38796)
                     /locus_tag="PLF14_03790"
     CDS             complement(37966..38796)
                     /locus_tag="PLF14_03790"
                     /inference="COORDINATES: protein motif:HMM:NF039422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOT70452.1"
                     /translation="MISEIIQSNQDRMLLVSGDKILCPDHMIQMLQHCFIDCLLPLKY
                     NKGDELIFDTLNGTSLSVYLKKKGYSPALLKKLLYSIKDMMRITGSYLIDAEYVVPDT
                     RYIYVFKDETFKYILDPFNKYDLNNGLKQLVSEITEGNYIDRSIKEALSSEHFDINGI
                     IKASYDGNIDVQEKVCEEPKKAVFKNIVDTDDCIIIADKDITVGRLKLICDYRLPGKL
                     ASLRHARMRYSHDTVYVRDLGSKYGTYLNGDRIKSESFVKVKRGDIVSFGEDEYILIS
                     "
     gene            complement(38798..38971)
                     /locus_tag="PLF14_03795"
     CDS             complement(38798..38971)
                     /locus_tag="PLF14_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp1 family type IVb pilin"
                     /protein_id="HOT70453.1"
                     /translation="MRIENGKKGMGTVEVIIILAVLVGIALIFREAITNFVQGIIDGT
                     LNNQSILEKLRVK"
     gene            complement(39014..39838)
                     /locus_tag="PLF14_03800"
     CDS             complement(39014..39838)
                     /locus_tag="PLF14_03800"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOT70454.1"
                     /translation="MIFFAIYILLILFTLLYSRKVRFDFPLCHCTFMKATYKAFVPLA
                     KVVTRFTASDNRLDLLYPKTAKEDLHITDICLKMNIFVIMLFLPATAIACKASAVVTA
                     ISVLLCFASVKCVDFDISKVYEKKKQEIITDFSVFLTKLTLCLGAGMTLYQAFTRQDY
                     DLPDRTFKVHLDRLINNLTMHSDPEYSFLELSLMLPIPQINAFCSIMITGFKNTEAGI
                     KDTLKTYTSSIWTERRNNAKKRAEQSSAKAVLALAIGLIGILLVLTAPAMIMLMSI"
     gene            complement(39835..40545)
                     /locus_tag="PLF14_03805"
     CDS             complement(39835..40545)
                     /locus_tag="PLF14_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70455.1"
                     /translation="MKFEVIKRAVTTGLVYAICVYFLYLSLIVSIITGVLVAGICLLD
                     KKYMKETVQKRHRDGFLRFLEILLSYTAIPSDFIHAFSKALLEFKRIYPKDYMLTLID
                     NIYSKVMANMSTDSILQSFAEHMDIEEAYLFCDSIIICEKKGADLNRTIKETIAFIKE
                     KRSIEQEIEVITEEKKTERLIVSLCPFLILALFNAMGTGYLDILYEGFLGRAIMTAAG
                     ILFLISSCLAKKILEVKV"
     gene            complement(40542..41699)
                     /locus_tag="PLF14_03810"
     CDS             complement(40542..41699)
                     /locus_tag="PLF14_03810"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOT70456.1"
                     /translation="MNWRKMMNNDYDKIIKQCLEGIAGMEEGEIEKTVTEYVYDIKNK
                     DMENKPEVIKRVMDAITGYGILQPLVEKEGITEIMVNADRIFYEEKGIIHRYDISFTK
                     QDIDKLISILCRKTDRTINMANPILDGSIGHDIRVNIILEPISHNGHSITVRRFPEKQ
                     ITYEDMIRDGFFTKEISDFLDNAVKERLNIFICGGAGCGKTTLLNVLTGFVPNSERVI
                     TIEDSVELMIDRIDNIIRLTCRNPNNEGKGEISMDSLIRASLRMRPDRIIVGEIRGKE
                     ALYMLQAMNTGHIGSISTGHANSCKDAFSRIETMVLMECDMPFLAIRKQIYDAIDMVI
                     SLQRIDGKRVLTEIVQVTEFEDGEIKTECLFTYDIESGKHIKVGEATRRRR"
     gene            complement(41674..42585)
                     /locus_tag="PLF14_03815"
     CDS             complement(41674..42585)
                     /locus_tag="PLF14_03815"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT70457.1"
                     /translation="MNKIKLHVKLSDNEYAGRLKDYIKINGLNVFALTEEHADVVLED
                     SGAENELLLKTSSKEWHTGKYQSADKIISAIMENIDISAGINVKSENRAIFVTSAHGG
                     AGKTFISQALSIYLSRNGRKVLYINLDGLCVKDSLFRCEQQNDISLIAYYASKGNENI
                     NTCIERYKNHDVAKNVYFLNSIYPSYDVSFDMNSAKVFINSLNTNSIYNYTIIDCPLY
                     PISPYAFIMKNAYKTLFIKCRGSEREEEVAAFLQKNDIRVMQLCNMIRYTEGIYIPKA
                     EEDITHNPYDFYEAIEQVVYELEKNDE"
     gene            complement(42859..44118)
                     /locus_tag="PLF14_03820"
     CDS             complement(42859..44118)
                     /locus_tag="PLF14_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase family protein"
                     /protein_id="HOT70458.1"
                     /translation="MTYKAYVTRVKDIQPIENADRLNKGFCFGNPVVIGKDVNETSLY
                     IYFPTDGQLSMEYAETNNLLRKKDAEGNSIGGYLDPEKRNIKAISLRGTKSDGLVMPL
                     SSLEPFGDITQLKYGDTIDEFNGHLICTKYIPKRSGQKQSGVKKSGIVKEIICPQFKE
                     HKDTEQLAFNSDAFRAGDIVEITLKMHGTSQRTGYLPVKKYRKNILHKIFKKQGNAYE
                     EYTYITGTRRVVLGSFDEEFRKIHADKLEGKLHKGETVYYEIVGYVNQSTTIMPACKN
                     SKLQDKEFSKKYGDETVFHYGCEPATSEMYVYRMTLTNTDGETVEYPYHLIQARCEQM
                     GLNVVPLFDRFIYTTEQDLLDHVHVYEEGQDPIGKTHIREGVVVRIENRVNFTAFKSK
                     NFQFKVLEGIVKESATAPDMEEAQEVC"
     gene            complement(44470..44988)
                     /locus_tag="PLF14_03825"
     CDS             complement(44470..44988)
                     /locus_tag="PLF14_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOT70459.1"
                     /translation="MTHYDIRPAIYDDLEILDDIFRGARAYMRKQGNADQWKAYPLLE
                     DIERDIRTGLCMVLVNRKGTPDAGEIEGVFFAQNFPEPTYDKIDGEWIDDKQSYITIH
                     RIASRGRKSGISDAAFAWCKERADSVRVDTHRLNVPMIRAIERNGFTYCGIIIIADGT
                     PRLAYQWVREQR"
     gene            complement(45055..45246)
                     /locus_tag="PLF14_03830"
     CDS             complement(45055..45246)
                     /locus_tag="PLF14_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70460.1"
                     /translation="MGEYYRSDDDMEKLQRKETASTVTEQVSDKKDYDFIGDLLEDSE
                     TLVKGIVLSEILGKPKAKR"
     gene            complement(45249..46247)
                     /gene="floA"
                     /locus_tag="PLF14_03835"
     CDS             complement(45249..46247)
                     /gene="floA"
                     /locus_tag="PLF14_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778175.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HOT70461.1"
                     /translation="MDPLIVVLIIVAVIIAFFSALLSLVPIGLWISAIAAGVKVSLTS
                     LIGMKFRRVQPAKIVNPLIKSTKAGLNVTINQLETHYLAGGNVNKVADALIAAQKARI
                     QLSFETAAAIDLAGRDVLEAVKMSVNPRVIETPIISAVAKDGIEVKAKTKITVRANMS
                     RLIGGAGEQTIIARVGEGIVTSVGSSESYKDVLENPDLISQTVLKKGLDAGTAFEILS
                     IDIADVDIGRNIGAQLQTDQAEADKKIAQAKAEERRVMAVAREQEMKANIEEMRAKVV
                     EAEALIPKAMAKAFEEGNLGVLDYYNMRNVMADTKMRESISEAGKAAKPQAIKKDK"
     gene            complement(46247..46759)
                     /locus_tag="PLF14_03840"
     CDS             complement(46247..46759)
                     /locus_tag="PLF14_03840"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOT70462.1"
                     /translation="MPGIFSFVENISILQAILLVVGMILLIIEAFIPSFGVVGIIGIV
                     LLLLSIFLTAKTFLEGVIMFLILLVIAVILLFIVIRIATKSRLSKKLINTDTFSEEQG
                     YLGVQDMSAKIGLKGKAVTILRPAGKAVFDGEILDVVTKGEFVEEGKEVTIEQVEGNR
                     IVVKEIKESV"
     gene            complement(46814..48574)
                     /locus_tag="PLF14_03845"
     CDS             complement(46814..48574)
                     /locus_tag="PLF14_03845"
                     /inference="COORDINATES: protein motif:HMM:NF027787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5050 domain-containing protein"
                     /protein_id="HOT70463.1"
                     /translation="MNRDSFINQATARLNEINVPYRVQDSADIAISGTFKKEDDDSAA
                     ILYAAFFFFNQKDNTVYAYERAMLNGVDISKDPSYGEVVVKNEDDDQSLLPLGIILTT
                     LAGIAVSLGANFKRTDDERLAKYPIIPASTPAATFCTNCGTKMPAGTRFCSNCGADSK
                     APVVIPQPKPFVQTTAQQRPFVQGAPPPVYAQPNVNQRMPSAKPKKKASVAVTIIVVL
                     VVIGILAGAGLLIRNLLRNGKPNGNNVAKPTASAGRIADPGPDPGPGTDTVSAVVSEG
                     ESADELGNIANSPYYFRTEDYIFYSCYDENSKAHIYRVDRDGKNFMSIFDGFGWSLLV
                     IDNKLYFCGNQGDTVDDTYNMFRMNLDGSGLETIVGEYCKGFFKFGDYLYYFKRNVEY
                     SHSFDLYRSKPDGSAEEQIYRWAEGATGHKNYLYFIDNQGNMIKCDPDGTNKDVILQA
                     DADIFLISNGKIVYMNQYYNYICTMDLDGNNNKCIKTEESKKMFGMNAYNGRIYYIAY
                     DPDFKRSTWSWSYDLVSMNFDGSDEKIIYSGASYGMYLNVVGGRVMVLDFKWIPETQL
                     TDVYIYSMNPDGSDLRKLQR"
     gene            complement(48719..49894)
                     /locus_tag="PLF14_03850"
     CDS             complement(48719..49894)
                     /locus_tag="PLF14_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HOT70464.1"
                     /translation="MRYTGVTSRGIITPIFKQGDNLVSMIADSVIEAAKNENFSIQDK
                     DIIGVTEAVLARTQGNYATCEQIAADIRSKFGGKDLGIVFPILSRNRFLLLLKAIAMS
                     CEKLYVQLSYPADEVGNSLITLDDLDESKVDPYTDSFNEVEFRKLFKNTAHIFTKVDY
                     IEVYKSTGKHVEIIFSNNPAYILNYTKNVLNCDIHSRKRTQKILRKAGAEKVYRLDEI
                     LTSSVQGSGYNEEYGLLGSNNATGDSVKLFPRDAQAFVEQLQKRMSDLTGKHVEVMVY
                     GDGCFKDPVGGIWEFADPVVSPGFTSGLLGTPHELKMKYFADNEFASLSGEDLISAMK
                     ERIKAKDNDNTESMGSLGTTPRRITDLLGSLCDLTSGSGDRGTPVVLIQGYFNDFAS"
     gene            complement(50004..51191)
                     /locus_tag="PLF14_03855"
     CDS             complement(50004..51191)
                     /locus_tag="PLF14_03855"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOT70465.1"
                     /translation="MSGTRLHRAVIEFARCIFEPYMKKKYGYEFEKVDYGKGNFIALA
                     NHTTNLDSIILGLSFKQHMYFVAGEQLFRMGWITWLLRTFLNPIKRMKAKTEARTAVS
                     LLRALKAGHNICMFPEGSCTWNGETATIIQSTAKLIKRAGVAMITYRVEGGYPSMPRW
                     SKSKRPGKMIGTIGNIYEPEMLEKMTVDEIYEAMCKDLYVNSIDMTEKYEYKGKTLAQ
                     NLETVLFVCPKCHGISTYTSEDDIFSCKCGVRLRYDTHCRLNDENNAGFPYTSVLEWD
                     NWQRQWVIDNIERMKKNPDKVITSDDNQELFTYTEDEKTEHVLSGSMALYTDRFVFKD
                     KKNKKELVINLSDITDIAIITMTTLTFSCSDIHYEVKCKIPRSALKYLLIAAALSPMR
                     YII"
     gene            complement(51276..51965)
                     /locus_tag="PLF14_03860"
     CDS             complement(51276..51965)
                     /locus_tag="PLF14_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOT70466.1"
                     /translation="MTELLDKIAPLLTEKSWLTPLSALLAGVVTSFMPCSLSSIPLVI
                     GYVGGTGQNDSKRAFKLSLIYVAGTAVTFTALGIIAALAGRLMGTAGSWWYIILGILM
                     VLMALQTWEIFQFIPQSNLLSKSTKKGGIGAFIAGMLAGVFSSPCATPVLVALIAIVA
                     TQNDLLWGAFLFMLYSIGHGVLSVIAGTSVGFVKKLSTSERYGKASKVISIVMGALIL
                     LMGLYMFYLAF"
     gene            complement(51962..52510)
                     /locus_tag="PLF14_03865"
     CDS             complement(51962..52510)
                     /locus_tag="PLF14_03865"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOT70467.1"
                     /translation="MKKPDKKIILKIVIPVVIVVAIAGIWFWKNYDRKSDIALNAPLV
                     QPVKEEHFALNASELDMEAWKSYNLPIVLDFGSDSCLPCVSFYPTLKTVHAEMYGRVI
                     IKYIDVYKYSKESSVYPVQVIPTQIFINADGTPYVPSKNIDVEFITYNLRETGEHAYT
                     VHEGVLTKDQFLSILRDMGVKE"
     gene            complement(52714..58653)
                     /locus_tag="PLF14_03870"
     CDS             complement(52714..58653)
                     /locus_tag="PLF14_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70468.1"
                     /translation="MVNDATHMFLGASNDPNSRTLVVLEQSNINVDRMSGNANFVPFD
                     GGSPTGIGLGASSVSLDQGPFYRTDANGNYIGNSFCIMGRRSPYEYYVWKTPSPGVFA
                     LALNTNNTNPRSPKPAEYTYPYLYEATGYDINVLIEEVNEIGDNLDVDMNYMITARVD
                     NPDSPTSFYALGETIDELNNKVICGTNVTSQAAAINNSNILDENNNIIEQHSTDMLIM
                     VPVEVDWHQTSSDKGLYFYQDYYSTNVVRDWLTASGSDIGITSINVNISTHPETQWYY
                     DGISKYMFYISGGIKYYLTYDITTNTFSLTTDMLAATHTYIYRFKPTYVVSRVTDAAN
                     DSLKSGDFIIAGRDAEGYTSLGIADDGLNLEARNVAEYIKETLTETERNEILNHIWKQ
                     HYYDFYTATYDPESTTQYMQLFSYITGGGFTIIHDTSTGGMDLSSDPMVWRITNNNGL
                     WDFINNRANGTVSANTRAIRFTGDTSNLTIRDSYTGPDRYIASGISCSFAATGYPIVL
                     CDTSGNPVTSPVNGTSYLLLARDNGVNLSAVKNSSGTISLVNYTSDTGQDAGCLWTAT
                     ENVGGWVLANAGRYISANSSGVITSATTANGTWSVSASGQLSYATYNFPYRQYTSGWF
                     GGERLRMTTDTGSYSARLYDYNSSTGTLTPTTSTGSNKVLLVKTGTTYYAVGMSYSNW
                     WGTSFSFTELSGVSVQPNGTITTTTGLSNTYTFSSATSGSGITLRNSSTNRYLTYSGT
                     SFGSDSSSGTTFYLDSNKYFYYSKGTVYTPYATQVFNTSPSDGITVYMYEHNSGVYTL
                     AENGLQNGKTYVMVLYDQSEYYLIGHDSFMLTRTSLGTTLPVDWTSNITSSVHHIFAI
                     DGPIGKYLYFSGGSSNNYLTVSNNYFMLTGTQTTQWEYNYSGILGSDSRLYSIISTNL
                     AAAHLMVGETNNIETTSTVMPVTLYQATDNGDETYTLTSKTSSGMPASGTYVITIYQD
                     GICYAVRYTSNGIIGYNLGSTVLEGSVIPDIVWTADGTSFKKTVGSQTYYIRRNQQGL
                     YLGTSQGATWTYTYNNVAASFTATDTTSTPLYIFRVDKSAELDDVTDTISMLSGKAKL
                     TQSVSLFESGNYMIVAEVDENGDSVVDRYYSLSMNDIYNSRAIDVTALMNLSLGFSSS
                     YVTFFDSSVWINQGTDLEVILKNRGYTDTYLLTGAQGNLEDMTPQIELVDDQSSVDLT
                     EYKWRVYTHTLGLNSVYLLGYVDTSTGIDTIYYMYFDSDNLQFKLTTDVSTAASMSGR
                     VQLYQVASMTPVQPAFHSYVIEVAEDGQTILSFPIKLVESQADIYKYTETQSEGQTLR
                     TGEYLIAAVVGHQYYTMTLSELLNLSYVDVNPYFSGNFSIDVDGNYCISVNSEYIWKQ
                     ISEPSIALNFENTKFEGYYLSSQTADFIYDIENRQLYNGSHYLSFDLNNGFTLSSTAS
                     TVPINLYLLGQVGVEVGLPGGELQYNYYTQPLTTIGGAVDFTHFDFKKTHMPDLKRYG
                     INYDGEIGSVPGWTLYDGVEILSVAEDSVFFCDGIDTTTDVTEMKNEFIPMTHSTEYD
                     GNPVSIPYYAPAGTASFVIDQASESNPVFVNVIATTELDNILCDPEYLRYLCLWKTAS
                     IDLETQQATPVKTYGDGLDEASNYAHTFITNRNTPYAAIPLPNIYGSEASGASYARVD
                     EFDYTLSDAAYGENYFIAHTFVITQPGVYYLGPTYGSVAFTYLAVDNRALNEEGETSG
                     VGFNDQFTIDFCYGDIETSVSGLVPNEAIGDLVYVGHDSWFQSNIHPQWIRGTADNPT
                     DYLYVNVNRTKSDVDDTSSVNFTAYTTAPIAPGILHVNNNSSLQRLTRKADFQVYCNG
                     LDAAAFTGSDDRLWVCNLDGEYKLFSATVNHESEIYKYYLAVSESAVADVLPADKPVL
                     STTVKYNWNVTDGYLMTPEGMYLVKPSGGYGLSSTSANAVGVYTYDGNLNLITEPVNG
                     IPYLLIANDGANKCVTVNMTQ"
BASE COUNT        17138 a        12536 c        10738 g        18275 t
ORIGIN      
        1 gagcgaaata tacaagaagg aggagtatga acagtcactg actgtatcat acaagcttca
       61 tatggaactt tatatagaga atctttccaa gtactacgga gagaaacttg ctctggacaa
      121 attcacttat acctttaagg aaggaacata tgcgatatta ggtcccaacg gtgcgggaaa
      181 aagcactctt atgcacctta taaccgacaa catatcccgc tcgggcggac agatatttta
      241 tgacggtaag gatatattgc agttgggcag atccttcaga aaaaagatag gatttatgcc
      301 tcagtcacag ggcatgtatg accagatgag tgccaaaggt tttttaagtt acatggcgga
      361 aataaaaggg ataccgaaaa aagaagcaag aggtgttatt aatgagcttt tgtccgttgt
      421 caatctttcc gatgtaagtc ataagaagct ttcttccttc tcgggtggta tgcgtcaaag
      481 aatactttta gcgcaggcct tactgggtaa accggacata ctgatgctcg atgaacctac
      541 agccggattg gatcccgagg aaagggtaag aataaggaca tacataggta atcttgccgc
      601 atcaaaaata gtactcataa ccacccatat tacatccgat gtggaatcca tagccgatta
      661 tgtaatattc atgaaaaacg gacgtataga agcttttgga aaacccgaac ggatacttaa
      721 agaaacttgc tctgaaaaca tggaagaagc atatctgaag ataatacata cagaggggca
      781 aaatgcggga acttaagcga atcatagtat caaaaaccaa tattgtctgt atagcttttc
      841 tgatatttat gaatgtcttt atctttttct gggaatcaac acccaaagga gatatatgca
      901 cattaccgga atataccgac cgctatatcg aagtattgaa tacctgcaaa ggcatggata
      961 atgaagaagg catcaattat ctgaacaatc tgaagaaatt atcggaagga acggtattaa
     1021 ttccccgttg gttatacagc aaggatatgg aatacaagga agaagcttcc ggtttttatt
     1081 acaaaaaata cggcgagcag atgataaaag acatagaaag cggcaagatt gacacttcgg
     1141 aacaagcggt taagaaagca tatattacca ttgaagtatg caatatactt atcgagaaga
     1201 taaatcatct gacagaatac cccgaatata tcaaaacaat aagcgaaaat gccgagaata
     1261 tgaaatcggt aagcattttt tccgatccga attccttttc ctacaggaat attattaaaa
     1321 cagtagagga tttttccaag atacatatta ccgaactgtc attggctaat gaccttgcgg
     1381 tcaatgccct gtttaccgac agtatctccg attacagcat tctggcatac attatcttca
     1441 tagtcctgct catgattcag gaacgtaaaa aaggcctctg gccgcttgta tatgcctatc
     1501 ctgagggaag agcaaggctt gcaatcaaaa gagttgctat actgcttctc tgttcggtca
     1561 ttgcaacggt atttcttgta ggttcaagat tcttaaccgc agcaatagtg tatagagggt
     1621 tcggagatat gtccaggcct atacaatcca tgagcttgtt taagggagta tcacagcctt
     1681 ttaccgtagg cggttttata cttaacttct tcctgtttaa gattataggg acatgggtga
     1741 tatcgctgat actctacttt atgatgctgg ctatatccaa tacttcattg gcagttgcag
     1801 taacctctct gtttttggga accgagtata cactgttcgc tttaataccg gacagttatg
     1861 caattgccgt cttgaggttt ataaacatat ttgcattgat aaattacaac aggatatata
     1921 tgaggtatct caatattaat ataaatgagt atccaataaa cggacaaact cttacaacat
     1981 atatgatacc cgtagcaatg attctttttg caacggggtg tatcattatt catcataaga
     2041 agaaaccttt ttccactccc aatcctttac ttaaactgta cgataaaata cttaaactcc
     2101 gtgccggatt gtccgatcgt ttccggttgt tctttgctga gcttcataaa acactgtgga
     2161 tacaaaaagg agcttttgtt attatcgttc ttttattatg ggcaatattt gcttctgaag
     2221 caccaccgct aaatgtgtcg gagtatgacg gaaacctggt atatttcgag aatatgttta
     2281 caggagaagt taccgaagaa acgctaacta aaattgatga agaaacggtt attgcatatg
     2341 aggaaatggc aaagggaagt atgcacggag ccacaaaagt acaatatctt tccatgatta
     2401 aagccgaagc cctggctaaa aaaggcacgg gtttgtggat aatggacagt acaatatatg
     2461 atgcactgct gaacagaaat gttgagggtt atcaaagaaa aaccggattg atagccatat
     2521 tattcacaac ggttatgaca gccggcatat tcgcttatga gcatcagcag aaaatgaaat
     2581 atctcttatg cagtaccgat agaggaagaa ccgtacttgt gcgtaataag acattaattg
     2641 cttgtgtaat gacatgtgcg gtatggctta tcgtttacgg atttgaaata cgtaatgcag
     2701 taaaaattta cggaagtctt cattcactat ccgctcccgt tcaaagcttc atgagcgagt
     2761 ttccttacaa aatgactgta ttgtccttcc ttatacttat ctatcttctc agacttcttg
     2821 tacttctgtc gatgacattt atgatactgt tgttgtccaa tctcagtaac agagcaaatg
     2881 ctgccgttat aacatcattg gcggttttct ctttacctgc cgccatagtc ataatgggcg
     2941 gaaatattat ggagtacttt tccgtggtaa aactgctttc tccgcaggaa aatatacctt
     3001 cattaattct aagttacaca ctgtgcttta taccgggtat aatagcattg attgcaaata
     3061 ccgttatatg gaaaggaata agatatgaaa ataacaatag caaacgattc atataaaggg
     3121 tgcatgagtg ccatagcatg ttgcgacata atggcaaaag ctttaaatga cgctatgcct
     3181 gaatgcagtg taaataaata tcccatggct gacggcggtg aaggcacaac cgactgcctt
     3241 gtttttgcaa ccgaagggcg taaagagtat gttaccgtaa acggcgtttt cggaaataaa
     3301 atgaccggat tcttcggaat actgggcgat aatgaaactg ccgttataga atcggcatgt
     3361 gcatcgggta ttcagggaat accgaaaaac agactggacc ccatgagagc gtcatcatac
     3421 ggtacggggc agctgataag ggcggctctg gaaagaggct ataaaaagat aatagtgggt
     3481 ttcggaggca ctgctgtttc cgacggcgga atgggtacat tagccgcatt gggcatggtg
     3541 ttttatgata aaaacaagtc aattctctcc ccttcaggtg ctgcaatgac agctgttgta
     3601 agcattgact catcaaatgt tattaaagaa ttgaaagacg cacatattga atttgcctgt
     3661 gatgtggaga atgtatacca cggaaaagaa ggagcggcat atatattttc tcctcaaaag
     3721 ggagccacaa aagaacaggt tatagaactt gatgcagggt tgcagaatct tgctgaagtt
     3781 tttaaaaaac aactgaataa agatatatcg aatattcccg gagcaggtgc cgcgggaggt
     3841 ctggcaggag gtcttatgac tgtctgtaaa ccggttataa aaagcggatt cgatgttatc
     3901 ttaagttata ccgatttaga agaagccata aaggacagcg atatagttat aaccggagaa
     3961 ggacataccg attcacagac cgtttacggc aagctaccca tgagagtggg aatgctctca
     4021 aagaagtaca ataagccggt aatatgcata tcgggtgcaa tatccaaaga tgccgaagta
     4081 ttgaaagatt acggtattac tgccctgttt tccataagca gaggccccat aagtcttaaa
     4141 gattcaatgc gtgacagcga aaaccttctg tatcttaaga cttttaatat tgcaggttta
     4201 cttaagttat tgtaatatat acaggcacgg aggtgtagct cagttggtta gagcgcttgc
     4261 ttgacatgca agaggtcatt ggttcaaatc caatcatctt caccagtatc atcccgcata
     4321 aagtgccatt ttggcacttt tatttttcgc attagtgcca ttatggcact tttatatttt
     4381 cacttggtgc catattggca ctttttctat tgacattaag gtgccaataa tttaaaatga
     4441 tgctgaggta atcttaaatg actataaaac aaatgcgaca ggataaaggc ttgacacagc
     4501 aaaaatgtgc tgattatctt cagattcccc ttcgcacata caaaagatat gaatcggatg
     4561 agagtaaaat caaagctatt aagcaccgat atattaaaga acgcatagct gaatacggat
     4621 ttatagatga ggaacacggt atattaactg tcgaacagat taaagaaata tgcaaagatg
     4681 tattcgcatc ttacgatgtt gaatactgct atttattcgg ctcttacgca aaaggaaaag
     4741 caacggaaaa aagtgatgtc gatttattag tatcagtaca ggtaaaagga ataaagttct
     4801 ttgagttagt agaaaattta cgtgaaaacc tgaaaaagaa ggttgattta ttggatgcag
     4861 ctcaattgaa caataatccg acattggtac aggagatttt aagagacgga gttagaatat
     4921 atggataata taaaggatga cactaataac attgaaaaga ttaaggcggg aatgatttat
     4981 tattagattc cgtaatgttt cgcattattc agattgcgga aaacaattca aaactttccg
     5041 ataaattcag agaaatacat aatgaaattc cgtgggcatc tatcaaaggt ataagaaata
     5101 taattgttca tgcttacggt cgcattaata tgacaacagt ctatgatact gttatttatg
     5161 acatacctaa catgtatgaa aaacttaagg atatataaag aagtctgata cctttagtct
     5221 cctctttttc ccattattgc tattgaacag ggaacaaacc cgtctatgtc aatacattga
     5281 acatcgcaat agccggcttc gataaaaaac tgtttatatg acgaaaaaga gaattctcgt
     5341 ttgaagtatt tggtgccctt acttattgct tcggtaaaca tattatttct ctttttttcc
     5401 tgtttaatga cataggatgt aataataatc ttgccattag gtttacacac tctatcaagc
     5461 tcatctaaag ctttgtaagg atcttccaat aaatgaatta cattggctgc aacaaccttg
     5521 ttaaaagtag catcggcaaa ttcaaggttt aaaatatccg caacggcaaa agaaatgttc
     5581 ttgtgatttc tgcaattata ctttgtcgtt tgaaccattt tctttgaaaa atccgtagca
     5641 atcagcttct gacacttagg tgcaatgctt tttgtgagca tgccggtacc gcaggcacac
     5701 tccaagacaa tatcggaagg ctcaaaaaga ttttcaatat gctttctcaa tgccttatgt
     5761 accttagcat tgattatata aacaaaaata tcatagatac ctgccacacg atcccatacc
     5821 gatgcttcat tacccttgtt ttcgtttttt ctctttttcc cttgtaaatc ctttttcaaa
     5881 cctatacctc tttattcact acaaacataa ccattacttt ttaattatca cccttataca
     5941 ttatgaaatc aagaacttgt caaaatccgg aaacttcctt tctcttacat ttttcagaat
     6001 gtctcaattt cttgctatta ctcatttgag tcttcctttg cttatctcaa tatcagtaga
     6061 caaatttttc ggccaattcc gttttatcaa ttaagtcttt gtatactttt gatgttttaa
     6121 gcaattgttc atgttttcct tgcgactcta ctatgccctc atcaataacc acaatcttat
     6181 ccacattttg aatggactta agtctatgag atattattaa aacagtttta tctttggtaa
     6241 gcttatttag gctgtcttga atcttctttt cattatccac atctaaagaa gctacaattt
     6301 catctaatat gataatgggt gcatctttaa gaaaagctct tgcaatagat agtctttgtc
     6361 tttctccgcc ggataagttg gctccgtttt cacctatcaa agtatcatat ccatcttcca
     6421 acttttcgac aaactcttca caattagcaa gagcagccgc ttttttgact tcttcgtccg
     6481 tagcttcagg ttttcctatc cttatattct ccaacaccga ggtatcaaaa agtgtaacgt
     6541 cctgaaatac tattgaaact ttatcaaaaa gtgaccttgt agagatatta ctgatgttct
     6601 cacctgaaat caaaatctct ccgcctgtaa cgtcatataa ccgtgacacc aatcgcaaaa
     6661 tactggtctt tccgcaaccg cttcttccta caagtgccgt aacttcattt tgttttgcta
     6721 caaaacttac atcttctaaa acctgtacat tgtcatcata tgaaaaagac acatttttaa
     6781 gttcaatatc aaattcggac aattccagct cctcaccttc ctgccttttt gtattcctta
     6841 tctcattaat tcttttaaca gctgaatcta cggaaaaaag ttccgccatg ttgaaagaat
     6901 ttgcttcagc accttctttc atcctgattc cggcaagcaa gtatcctaag aaatataata
     6961 tatttatttc tcccttgcca agaagagtta ttcctacaat aatcaccaaa atatgcgtga
     7021 ggtaaagaat aagacttgcc aagcttatga ttgcagcacc gacaaactcc gattggatat
     7081 gaattttttc tccttcttcc acttctctat atagtttttt ctttacatca tcactcatcc
     7141 cgaaactttg tatttccatc tgaagttcta tagcctcctg gaaactgtcg gagttttgtc
     7201 tgcgtttttt atagtattta tgaaaatttt tcgtttgcac ttttttagaa attaacaata
     7261 aagtaaaata cagaataatt ggagaaataa cgcaaagtcc gagtttatag tttcctatta
     7321 gaagcattac tgctgctata ataaagaatc cggtaaaacc tataatttta gcaatggcat
     7381 gggaaagagc atgttccaat gtagcaacat ctgacatgat agtttgtgcc aaatccgtta
     7441 aatcgtgctt tgaaaaatag gaaagcggta aatcacttat atttctggca atgtcaattc
     7501 gcaagttctc actttctctg taagttgtat taaaaagagc actatattca atactgagaa
     7561 gtatatataa taaaacaata actcctatag aaaagacata gtagaatatg ttatctcttt
     7621 catttaacaa taatgtttta tcaaaaaaca acatcatcag cattgcggga agcatattaa
     7681 caatgtatgt aacgaatgat gcagtgctgg attttataag gtcgtttgcg ccttggtccg
     7741 ttaatgcaaa acttttttta atgaacgctt tcatctgcca ccctccaatc atttgcggtt
     7801 ttataggtat cttgaaagtg cttataaacc gaatcttttt tcattaattc cctatcactt
     7861 ccccgctcga taatctttcc gttttccata accaatattt catctacatt tcttatactt
     7921 gttaacctgt gagcaatcat tattacggtc ttttccttca tcagattgga aaaagccttt
     7981 tgcaattcat gctcgttatc cggatcaatt gccgcacttg cttcatcaaa aataactatt
     8041 ttagcatctt taagaattgc tcttgcaatt gcaactcttt gtttttcccc tccggacaaa
     8101 taaactcctt tactgcctat aacagtattt tctctatcct taaacttcgc aattataccg
     8161 tccacacctg ctaaacgcat tgcttcaaat acctctttgc ggcttgcatc tttgttagca
     8221 agagcaacat tttcataaat tgtagtctta aaaagttttg aatcctgaaa aacaaaagat
     8281 atctcattaa tcaaggcttc tttgctgtac gatgtgatat tctcaccgcc tatctttatc
     8341 tctccgtcat ttacgttata gaaaccggaa atcaactttg caatagtggt ctttcccgaa
     8401 ccggaagcac cgaccaatgc ataggatttg ttttcttcaa gcttaaaact taagttttcc
     8461 aatatcaaat tatctttttt atacccgaaa ctcacatttt caaactcaat attatggttt
     8521 ttaaattccg ttttgtctcc gaattgtaaa acatcctcct gcatatcttt gtacatattc
     8581 tccaactttg aaacagcgtc ttgagcttgg aatgtataca ttgaaagata cataactttc
     8641 ataatatagg tataaagaac cccgcttaag aaaaacacca tgataagttc caaactcata
     8701 agtttcaaat cagaacccgc cttcgtaaaa agcagtataa caggaatcaa aatagctaca
     8761 acgccaaaga acagttcctg aaaaagcaca tacggtgttt tgcaactgag tgaataatta
     8821 agagcatttt gagcatactc ttttatggca gtatttagtg ccttaaaact tcttacatca
     8881 acaccgaata tttttacaac ctgcatcccc cttatatact caactgtttc ggaactcaat
     8941 ttttccaatg aagcctgata aatcttcata aactgttgtt cacccatcat atactttagt
     9001 aaaacaagac taacagcagt catgacaacc aatacaattc ccaccctgta acttatgaaa
     9061 aaacctaaga ctaaaaccag taccggcgta atcatagctc ccgtgttgtc cggtataagg
     9121 tgggcaaccg ccatatgtgt ttgagcggca ttatcatcaa tcagtttcct tattcttccc
     9181 gacgaattta aatcaaaaaa cttgaaactg gcattggtaa gcccgtcaat gccgaatttt
     9241 cttaagttgg tttcaattct gaatgcaaac aagtgagaaa gtatcagtga caaaaaatac
     9301 aataatcctc cgcctgctaa aaatcctgca attaaaaaag catacctttt tgcattactc
     9361 atgtcatcga gcaagattac attttccaaa agcttgtaca gatggtaaaa tgccaacact
     9421 tgaaggaatg ctgcaaaaac cgaaaacaca atcgttagaa aaagaaaatg ctttttttcc
     9481 ttcccgtagg atatcaattt tttataaaca ttacccatta aaaactctca tcctttacta
     9541 tttcttttac agcatttatt caacttatgc tgtcttatag ttaacggtta gccgctgcta
     9601 accgttaaca gaataacacc atcactttta gttgtcaact tcatttttag acaaatttcc
     9661 gatagcgatt ttgaatctgt attttgcatt caagaaattt ttattaaaaa aatataatcc
     9721 tctttgccca attacaaact gttcagaaat cttacaatct catcgtaggt tctcttagta
     9781 gccttgttta ttcttacaac accgtaacaa tgaggcatat ccggctctat atgaattcga
     9841 tagtctgctt gcgctttttc aaaagcttgc ctgtaaatag gagctgcaaa agcaaggctt
     9901 tctttcttac cgtaatacac gaggattttc ccggcattgc gaaagtcccc atgtgcagta
     9961 tgcacaagcc aatgttccgg ttcggtcttc cctttttgaa tactgccata agcctccacc
    10021 tgttctatcg gaataaaggg gtcaacacct ctataagctt caagcatttc tttttcagtt
    10081 gaatctgccg gtacatgacc gggagaaaga gcaactgtaa tggtcggcat gggaaggttt
    10141 tccttgtaat aattgttcca agaaatcata gtaatggctg ctgttccgcc aaaagatacg
    10201 cccacaactc caatcttctc attactgtag ctgttcaaca tagttctata ggtattaaaa
    10261 accattttat aagcttcatt tacgggatac ttggttgaag gaggataaaa cggatagtaa
    10321 atctcggcat caacccgctt taaaagtttt cttgcaagga aaatttggtg acggcaataa
    10381 tcgtagacgc ctccacctcc acatatgtat aggatagctt tgttgggctt ttgtcctttc
    10441 tttttcatgc gaaggcagtg gtaggtatcc gtctcggtat gaattttaag gtcgctatac
    10501 tctattcttc catttctcgg aaccctaaag gtctgcttct tgttatgtgc tttagcatgg
    10561 gcatatattt catccgtgtt ttttagcggt ggatttaaaa aatgagttaa ccaaaggctt
    10621 cccataatta ttttattgct aatactcata taaatcctct ctccaatgca ttatttatat
    10681 ttatcgacat acaatgtctc ttaatagatg atacggtttt tcttaataca cgtcaaatca
    10741 ccgacattct tcttcaaccg gatacaacct ctataaaatc cactttctat taataatatg
    10801 tcggcatata aaaaacagta tagccgcaaa aatcagcaga attccgaagg acaataacgg
    10861 agagagcata ttatcacctg taatagtact tttcagaata tcggttccgt aagtgagcgg
    10921 aagacaaaat gaaacaggtc gtaaaaatac cggtaaactt gaaatcggta taaacagtcc
    10981 gcagagaaac agcataggga aacgaaagaa gtttgaaaga gtctgagctt caaaaacctg
    11041 ttcagccgat accgcaacca ataaccctgt caatgccgag gttatggcaa tcatcagaat
    11101 tgcaaacaaa agaacttgcc aatttattgc atgaagatca ataaaaaaac ttgccagaat
    11161 aatcggaaca aatgcattaa atataccgaa cagaattgtt ccggcaattt tcgccaacac
    11221 cagcaagctt aaacttatgg gtgcaagcaa aagtcgttca aaggatttac cgctcctctc
    11281 aaaagtaatg gtaacagcca acatagaggt agtgccgaac aaaacgctca ttgcgataag
    11341 accgggaagc aattccgaca tatctgctcc gggtgagcgg ataaattgca taagagacca
    11401 tgaaagcgga aaaatgattc cccaactgat atttggcggt tttaaataat agttctttat
    11461 atcttttcta agaatattcc aaaatgcaat ccaacttttc atcgcttgtc tcctttttct
    11521 ttgtcttttt tcatcaaaca agattcaatg cctgtcagtt ttacgaatac atcctccaaa
    11581 gaaggatgta attcccgtgc ttcatgcacc tcaatcttgc ggtcatgaag aaatttcagg
    11641 accggaaaca gcggaatgcg ttgttccgat tgcattacaa gagaatggtc ggattgagaa
    11701 gtaattacac ttactttaaa tcggttttgc aattcaacgg ataaatttcc aatgcattta
    11761 tctgttacta accgaactgc ataaccatga gaaacacttt ccatcaattg cctaactgta
    11821 ccggttgcaa ttattctacc tttcgctata aaagcaatac ggtcacagat gcgctcggcc
    11881 tcttctatgt agtgggtagt cagaaaaaca gtggtcccct ctgctttcag tctcactatc
    11941 aaatctcgaa tctgtccggc actttccaca tcaattcccg ttgtcggttc atctaaaaac
    12001 agaatttgcg gagaatggat aagtgcagcc gcaatggtca gtttgcgacg cattcccttg
    12061 gaatatgcct taaaaggtcg ccctcccgta tcattcaatc tgaactgctc taaaagctga
    12121 tatgctttac cttctctttc tttttttctc attccataca aagctgcaca aaaacacaga
    12181 ttagaaaatc cgtccatttc attgtagaga ttactttcat ccggcacgat tcccataata
    12241 gcctgtgcct ttttagtttg cttcactaca tcaatatcag caacagtaat cgcaccctcg
    12301 gtaggtcgcg acagacctgt catcatatta attgtggatg ttttccccgc tccgttaggt
    12361 cccaaaaaac cgaagatttc acctttttca atcttaaaag agatgtcgtt tacagcaaca
    12421 acattaccat atgtcttttt aaggttttca acattgatta ttcccataat ctctattttt
    12481 ccttaccttg agatttaccg tgacaaaact ctctgacagt ctcgctgcat tgccctttct
    12541 taggacattc tttcttttca tcagccggac aatctttgcg tatctcaggt tcacaagttc
    12601 cctgttgaat catagccaga gtatcaattt cctttgccaa ctcacgtaat gctgcccgct
    12661 ctacagtata gtgcataaag taaccgcgct tttctcctac aagcagtcct gcctctctta
    12721 acactttaag atgttgagat atggttgctt cggttaattt cagttcgttt gccagtgctc
    12781 ggacacaata attgtgtgac agaagtaatt tcacaatgct catacgcgtt tcatctgcaa
    12841 tagctttcag tatcttcttt ttatccataa taaccctcca attatttaat aaggcgaccg
    12901 cctaattaaa ttatattaac acatccaatt tagtcaatac ctaattgaaa actttgctat
    12961 gattaaaata tattttccac cctagttttt tctatttgca gttttttcta acattagata
    13021 cttgctttta tcacccacct gtgcgataat gtgggcgata aggtgggtga taaaatgttc
    13081 tccgttaaaa ccatagttaa tgatcttttg caatacacat ccgaaagaga atggtttgag
    13141 tttaaagaaa actggtatga accgtatgaa attggtcaat atatctccgc actctccaac
    13201 agcgcggttt ttgaaggtcg cagatatgct tatcttgtgt ggggtatcag tgataaaaca
    13261 caggaaatag tcggcacgaa tttcgactat aactcctctg taaagaatga acctcttaaa
    13321 cattacttat ccagacagct aagtcctttt attgatttca gttttgatga aggtatgatt
    13381 cagggcaaaa gagtggtgtt gcttagaata ccggcaacca aaacagtacc tctttctttt
    13441 gataacgaaa gatacataag aatcggttcc agtaaggaaa aacttcgcag ataccccgag
    13501 aaagagtcca atctgttcta ctatttaaga agcggacatc ccacgattga gaacactcct
    13561 tctgcctatc aggatttaac atttgagaaa ctttttatct actacggagc aaaaggatta
    13621 aagctcaatc aggatacttt caaaaagaat ttatctcttc ttaccgaaga cggaaaatac
    13681 aatgtattag ctcaattact gtcggataat tcacaacttc ctctccgtgt tgctatcttt
    13741 tccggaaaaa gcaagacatc ggaattgttt tccgttaaag aattcggcta tcagtgcatt
    13801 ttatacacac tggaggatgt attgcgttac ggtgatattt taaatattat tcaggcagat
    13861 gagcgtggaa gggttgttga aagaaaggat gtaccgttat ttgaatatga ggcattccgt
    13921 gaagcaatca ttaatgcttt tgttcacaac aaatgggtta gcggcaatga gcccatgatt
    13981 accgtatatt ccgacagaat ggagattctt tcaagaggca ccattgaccc agaacagacg
    14041 atggaaggtt tttttgccgg cgaatcggtt cctgtaaaca agaaactatc cgaaattttc
    14101 ttacagttgc atatcagtga aaaaaccggt cgaggtgtgc cgaaaattat tgatacttac
    14161 ggtagggctg catttgagtt cagagagaac tccattgtag ttactatacc tttcagatgg
    14221 attaatatgc cgggtgataa agtgagtgat aaaaaaacca agcaggcttt gccaaccagg
    14281 gacaggattt tttatgccat aaagaacaat cctgacatca ctataccgga aatagccact
    14341 ctcttaggag tcggtaaaac cactgttgat aacggtattt ccgtactcaa aaggaacgga
    14401 aaaattgaaa gaataggatc aaataaaaca ggttattgga aaattgttaa tcaatttaaa
    14461 aaagctgctc cattgcagaa ttccttcttg gtaagtgaaa aagaaagcgc ttatgaaaaa
    14521 ggtgaagatt aaagttaaat tttacccaaa ccgactcttt ttgttgtgca ataatataca
    14581 ggtgatataa tttatttgga aagtgaggtc tatataattt ctatgttcaa cttcttggaa
    14641 atgtttgaaa aatctttagg attgaaagaa ccatggaaag taaccaaggc acagttcatt
    14701 gaggaaaaca gaagtgtaca tgtatatgta gaagcagata agaacgcaga atacccgtgt
    14761 cctgaatgca ataaaatgtg caaaagatat gactatgagg ataaagaaag gacatggaga
    14821 catgcagatg ttatgcttta cccctgtttc gtgcactgca tgatgcccac agtcaaatgc
    14881 gaagaacacg gcataaacgt aaccgatgct ccatgggaaa gtataaatcc gttaccttaa
    14941 tactttcacg ggaaaaaata atttccaagg gaaacataaa atatagtcgg ttgcaaagct
    15001 ctgtttatca tcctatactt acaattgtaa tggattggaa atcaggttaa atatgagact
    15061 tttatatgat aaagtatatg aacagataag aaaaacggat ttcgaaagct tatggaaagg
    15121 ctttcatgca tatcattttg ctttatataa cagcgattcg gtctatttca aggacaaaac
    15181 cggtgattat gaagagtgtt tcttaggtaa taccagtata aaatacggtg atgagcagat
    15241 agccatatgg agagtggacg attatacgaa agaagatccc gtactgcttg cggccaatat
    15301 catacatgaa atgtttcatg catatcaata tgagtgtcgc gaaaagcggt ttcccgatga
    15361 cttaaaagct ttggattatc cgatagtttc ggataatata cttttgaagt atgcggagaa
    15421 tcagcagatt gtcaaagcgg ttaaaagcaa tgatagaagg attataaaag aatgtttatc
    15481 ctcggtgttt gcatccagag caagaagaaa agaattaatt aaagaatata tcgactatga
    15541 atacaagatg gaaaccgttg aaggtatggc tgaatatata gggttatgtg ctcttaaaca
    15601 gttggattat gaaaaatatg agaataagat aaacaaatat attgataacc ttaccgatac
    15661 cgacaatatt ctttttgaca acagaagatt ggcatattat tccggttgtt tactgttaat
    15721 tgcacttaaa caagcggaca tatgcttttt ccatgatatt tcaaaagaaa ccaaaaccgt
    15781 atatgagttg gtagcagaag gatttcctgc tgccgattta cctgagattt tgcataagga
    15841 tgctattgaa gaatcgttga agcgttatat tgataacagg aagaagctgt tttccgattt
    15901 cttttccaag aatcctgagt gtacggaaaa atcctttaag atttgcggat atgaccccat
    15961 gaatatggtt aaactggaca accttgtatg gtgcaaatat ttcgtcatgc tgaaagaaaa
    16021 agcaaaggaa gatcctttgc ttatatacaa tcaggttgtt cttaaggttg acgagaccga
    16081 tacaacctcg gtgtcggctt actataccgt ctgattttta tgcttttgat gcaaccttct
    16141 ttttgcgtct tatgaggtta ttagccttaa tccacatata tgtgtcttca atagactgtt
    16201 tcaaggaaaa tttcagctta tatcccaact cctgttgtgc ttttttggag ttgaaattgg
    16261 aattggactg aagagtatac agtgaataac tggtaaataa aggcttctgt ttctttatct
    16321 tgtaatacag ttccgcaagc ggagccgtca ttttggcaaa ccaaagagga agaatggttt
    16381 tgatttgttt cttcccgaga accgaacata tagtgtcggt aattttcttt atcggataat
    16441 attcccccga taagatatag gcgttaccgc ttttgccttt ttttgcagct aatactatac
    16501 cttcggctac atcgcgtaca tcaacaaaat catagcctcc ttttacacag gctgtcagtc
    16561 ttccctcggc acagtctttt acaagctgtg tcatatgacc gccggcataa tctcccggcc
    16621 ctataattcc cgacggatga actaccatgg catacaagcc tttctgaacg ctgtccatca
    16681 ctatctttgt ggctatggct ttggttttac tgtattcccc ttctaccaaa tcgggattga
    16741 aatccgatat ttccgctatc atttcgtcct tcggtaattc cggaatggca tgaacggtac
    16801 ttacatagac caattttttg attttatgct ttaatgccat atccacgata ttttgagttc
    16861 cttttacatt aacatcatac atcaactggc tgaactttga tgatatcgag acaatacctg
    16921 cagcatggat tactatgagc tcctgatcgt cttcaaccgt aaagaaatct tccattgaag
    16981 cagcatcaca tacgtccgct ctgaaaaatt gcacatctct atcctttaat aatccgtact
    17041 tgtcttgtcc tatatccata actctgaccg tcttcccttt ggaaagcagg ttatccacta
    17101 tagcatttcc caagaagccc aatgctcccg taactgcata aacttctttc atccgttcat
    17161 ctcctttccg tacttttaac atacatttag tacattctac atgtatgata tacggctaag
    17221 ttgaactgat tatgaacaaa taaagggatt atgatataat ttttttagag gtgcgacatg
    17281 gataatccct ttatttctca tttaagaaaa ccgcctaaac tgaaaggtat taaaaaagct
    17341 cttttcatac aaccacatgc cgatgataac cagataggtg caggcggtat gatggcatta
    17401 ttggcagatt caggagccga agtctatgag cttacggtga cagatgacag aagattggag
    17461 caggacaaag aaggtctcac tacccggcaa tcggaagcag ttgccgccca gcagtattta
    17521 aatgttaaaa atgccggatt tttaggtttc tgcgacaaaa cgcaagaacc ggtggaaaag
    17581 attgctttga agataatgga ggttataaga aagattaagc cggatgcggt ttttacagtg
    17641 gacccggatc tttctaccga atgtcacagc gatcatataa aaacgggtat ggcggtaaaa
    17701 tatgcggtca tggatgccgc atgtgatttt tatccctatt ccgaagacgg taaggcacgt
    17761 catgaaacat ggacaacacc gatacttgca ttttactaca ccgataaacc caacagaaaa
    17821 gcggatatat caaaggttat ggacaaaaag atcaattcga ttaaatgtca taaatcccag
    17881 gttgactcca atcttctgat gtttgtaaag gaacttgcta agatagcggc aaaaggaaca
    17941 aaattcaagt atgcggaaca gttcagagca ttatcttcac tgcagatgca ctgctttaat
    18001 attgccttag aggaagataa cgactgatta gaaaaaacaa gtacttatta atagctaaaa
    18061 atcctttaca aaagaatatc ctataatctt cttatctttt tctatcaaat tagcataatt
    18121 tacattatcc taattcatat tatgctaata attttgattt tccaaccgga agtaaagcct
    18181 attccatcgt cttaataatt cgtccgactc aattgtgaca ccggcggtga cacttttttt
    18241 tctcaccttt attaagttca taaaaccgct tcatttgatt aaaacaaaca cgcacctgtt
    18301 aaggtgcgtt gttgtttgtt attaatcctc tacgggtagc ggttcgggag taccgaataa
    18361 atccttattg ctgtcgtcta taaactggct ggtgtaaagg ttatggtagt gtcctttcag
    18421 tttcataagc tcttcatgac ttccctgttc tattactttt cccttatcaa ttaccaatat
    18481 cctatcggct tcccttattg tcgacagtct gtgcgcaact acaaaactcg ttctgttatg
    18541 taacaccttg gaaatggcac gttgcaccgc tttctcggtc tgcgtatcca cagaacttgt
    18601 ggcttcgtca aggacaaata tacagggatc attcactatt gctcttgcaa tgcagataag
    18661 ctgtttctgt cccgtggata ttccgccgcc gcctttggag ataacatgat tgtacccgtt
    18721 ttcgaatttc attatgaaat catgggcttc caccatcttg gcagcattaa tgacttcttc
    18781 ctccgttgcg tcttccctgc cgtaacggat attgtccatg attgtcccgc tgaaaagatg
    18841 cggagcctgc agcatgtagc ccaagttctt gtggatatac atcaaaggta tcgttttata
    18901 gtcttgcccg tcgatatata ttgtcccctc ggtaggttca tagaatctgc atgtgaggtt
    18961 aactatcgtt gtttttcccg atccggtttc tcctaccaga gctatgcact gacccggttc
    19021 cacacttaag ttgaaagtgt ctaaaacctt ctcgccttct ttatatcgga aagacacatt
    19081 ctcaaatctt atggaacctt tcagtttagg taccggcata tccgatgtaa tatttccgta
    19141 tttttcaata acctcatcgg aatcctttat gtctatatcc gcctccagca aagccataat
    19201 tcgttcaccc gcagcctgag aaaactggaa tctggcaaat attcttgcca tatcgtttac
    19261 cggatggaag aactgtaaag tgtacgatat gaacagtgta agcgttccga ccgttatcac
    19321 ttcgcctaaa atcattactc cgccctgcca tatggccaac gaggttccta tgatacccaa
    19381 taccgatact atcgggaaga acaatcccga atatactgcc gttttgatgg aagcggtcct
    19441 catgtcatcg gttttctttt caaagtcgct gatgtggctt tcttctctta caagcgtttt
    19501 tgaggttgtt gcaccctgta ttccttcgga aaaagcgccg gttattatgg aattgtatct
    19561 tctggcttct ctgctgtaaa acagtattct tttttggaag aataccgata ttactgccag
    19621 tatcggaata acggtcagca ctaccaatgt aatcttgacg tttttcatga acatgtatac
    19681 cgtcatcatt attatcatgc ttatcgccca aatcatgtcc acaagtcccc aggaaatgat
    19741 tcccgtcagc cttccgacat cggaggttac tcttgtaaag agactacccg ttgcatttct
    19801 gtcatagtag gaaaagctga gttcctgaag ctttttaaat ccgtccaccc ttatatcatg
    19861 actcaccaga tgccaaagct tgtctgcaag atatatgaat gtatatatag tcataccttt
    19921 cagtatcagc aatagtacat ataagattat aaacaggcca agcccttcgg ttgttttata
    19981 ggctatgaaa ttatctatcg catattccgt aaaaagaggt aaagccgcat cggtagccgc
    20041 atttattatc atggctatga caatacctat aacatggttc atgtattttg cgagatactt
    20101 tattgccttt ttccacatct tcaaatctat atttttatta aatatttctc tgttatcttc
    20161 ttccatacgg tttactcacc tgccttcgcc atatccagta cgcagctctg catatcatat
    20221 acctgcttat acaatccttc tctttgtata agctcatcat gcgtacctct gtccgttata
    20281 cggccgtctt ccattacaag tatgaggtcg gcctgtctta atgtatttat tctgtgtgat
    20341 atcagtattg tagtggtatc ggcacagtgc tttctcaaag cattccttat ctccttatcg
    20401 gtctgcatat ccactgcact taacgagtcg tcaaaaatca gcactttgca gttgccgata
    20461 agtgttctgg ctattgcaac tctttgtctt tgacctccgg acaatgtaac tcctttttca
    20521 cctactatgg tatcgtagcc tttttcaaag ttccttattg ccctgtctac cgacgctatg
    20581 cttgtggctt tttcaatatc ggcatcggtt atatcgggat tattatccga tatattttct
    20641 ttaatggttt tggagaacaa aaacggttcc tgcaacacga tccctatatt cttcctcaaa
    20701 tgccttttat ttatttcacc aagttcaatg ccgtccactt ttatacttcc cttctcatac
    20761 ggaagcagtc cgacaagtaa gtgcaacaaa gtggattttc ccgaaccggt ctttcctaaa
    20821 atagcaagag tctctccttt tcctatactg aaagatatat ctttcaacac ttcgtttccg
    20881 tccgtatatt catacgatac attctcaaaa gatatattac cttcaatttt cggggtaagt
    20941 ccggtatcgt cacgtccggg ttccctggga gcttccagta cttcctgtat acgacccaat
    21001 gaaacacttg ctttacccat atctcccaaa actcttccca tgccccttat gggccagatg
    21061 agattatgca cataagtcat gaaaaccgtc agcataccca ccgttatctc tcccttaaca
    21121 gctaagataa ttccggtcac caatacaaca cctatctgca actgacatat tgcatcggaa
    21181 agtccccagt accatgcaaa tgccaccgtt atcttcaaat ccttgtttct gtaatccaat
    21241 gaagatgcga taaacttttc tatctccctg ctttgtgtag cacaggaacg tacaattctc
    21301 attcccgaca ggttttcttc tattaccgtg gacataactc cttccgcttc atcggcaagc
    21361 aggaacttct ttgatacata tctaaagaag aagaacgctc cgagcactat tatcggcata
    21421 agtgagaaac ctgccaacgt taaatcggta ttcactttct gcatcacaga aaccgatgcg
    21481 actatgagga cgatacattt tatgaggtcc tgaaactgtg tagccagaaa attcctgacc
    21541 gtctccaggt cggatgtgca tctttgcata atatctccgg tctttatact tgtattgtac
    21601 aaatatgtgg agttctgaag atgctcatac aaatccatac gtatattttt tgctatacct
    21661 tcaccggcaa ttgccgaata ctttctggat aaaaacatga atacaccttc aaaagccgag
    21721 aaaaacacca gcaccagtcc gcaaatccac agattgtgca ttaaatgctc tttgccgcct
    21781 aacagttgca atcctttgta tgcaatacct tccggcagta tatcccctat aaccgagtct
    21841 atgacgacac ctattataaa tgtgttaacc aggcttaaca actgggctat aatcataaag
    21901 actatacccg ctatgtatgc cgctttgtta ccacgtaaaa attgttttaa taatctatat
    21961 ttcttcacaa aaaacaaccc tcttgtcttt attttccgac ctgagggttt tacgcataaa
    22021 acatatatgt cataaagatt ccacaggtca ccgtttcgcc tgcagaatgc cgttcaacta
    22081 ttctgaacaa gccacaggca tacctatccc gtcattcaat gctactgtat ttctgagttt
    22141 gatcatcgta tgccccttcc tttcaagtta atcttttttg attatataca acatatttta
    22201 catagtcaat gcaattttgt tgcagttgtg taacaattca aacaacattt cgtaaatcat
    22261 atagcggttc tccctttcag tgctcttatc aatgttgcct catcggcata ttctatctcg
    22321 gtacctaccg aaataccgtg agcaattctt gtgactttta tgttgaaggg ctttataagg
    22381 cgggaaagat acatcgctgt agcctctcct tccaccgaag aattggtcgc tattataacc
    22441 tcttttacat ttccgtccaa cctcttcaac agttccttta tctttaaatc ttcgggattt
    22501 acaccttcca taggcgatat tattccgtgt aatacatggt acacaccgtc atactcgcag
    22561 gttctttcca ttgccataac atcttttacc gaagacatta cgcaaataac cgaatggtct
    22621 cttgaggaag aacatatttc acacgggtct ttctgcgtaa tattgcagca gacggaacaa
    22681 taggtaagac cggctttggc gtctcttatg gattccgata tgtactgaat tctttcatca
    22741 tccatggtaa gaagatgaaa agccaaacgg gtcgccatct tcctgcctat ccccggaaac
    22801 atttcaaacg cagctattaa tcgtgataac ggtttcggat atatatcagc catttagaaa
    22861 ggcatcctca tgccgccggt cgctttaccc atagtttcct cttttttggc atttatcttt
    22921 cctaccgact cgttaacggc agtcaaaatt aaatcctgaa gcatctccac atccgcatct
    22981 tccaaaacaa ccggatcaat agttatggag tccattgtat agtcaccttt catagcaact
    23041 gtaactaccc cgcctccggc tttggactcg aatgtgctgt tttccaattc ctcctgtgcc
    23101 ttggcaatgt cggcctgcat cttctgcgcc tgtttgagca tactgctata gttcatattt
    23161 ccgccgccca ttcttccgcg tgccatatta tctacctccg ttatataatc tctacgggta
    23221 tattgtgttt cttcccgatt tccgttattt gctgttccgg tgagccggca actttgtcgg
    23281 taccggcaac cacgaacctt gcatttgtta tatccttact gtatcctcta atctcttcca
    23341 acaccgcaac tccgtttctt tccaaactgc tttttaaaaa atcatttgct acggttacaa
    23401 ccgcctgatt cccgcttatt tcaatggtcg attgtaataa ctcaaataca tctttggaac
    23461 ccttatcaaa cctgtcctgt atatatttgg accaggatat attctgattg gattttgttt
    23521 ccgtcttaac cggcttagaa gtttcgttga cggccggttt gacggcatcc gccttttgca
    23581 cttcctttct gacaggttta tccttaacct gcactttagg ttgtactttt tcagtatttt
    23641 tcactaacag gcccaacagc attatttcca gatatgtttt aggtctggat gttcttttga
    23701 gtaccgcttc gttttccgag agctccttta tataccatat aacctcctcc ggctccataa
    23761 gcttggaaag tcttttcatt tcgctaacgg cattaccgtc cgcctctata aaccggtccg
    23821 gctctttcac attcatgcat accaaaatat tacgcaaata ccctaataac cctgatagaa
    23881 accctgcagg acttttccct tcagctataa cttcattgac actgttaagt attccggtta
    23941 catcgttatc caccaaagcc tgaaccacct ttgcattgga catatcatca ataaggcctg
    24001 cacaattcaa aacatctgta acatcaatcg tatcatctgt tgatgatata cactgatcca
    24061 acagacttat tgcatctctt aaggctccga aagaaattct ggcaatcata tccagggcct
    24121 catcggtaat ctgctgtttc tcggcatctg caatctttcg caaacgatta cttatccctt
    24181 ttgttgttat tctgtgaaaa tcaaaccgct gacaccgcga cagcaccgtt gcgggcagct
    24241 tgtccggttc ggttgtgcag aggatgaata tgacatgggc gggcggttct tccagcgttt
    24301 taagcaaggc gttgaatgcc gatgtactga gcatatgtac ttcatcgatt atatacacct
    24361 tatacttcgc caacgccggc atatagttta cttcatcgca tattcttcgg atattatcta
    24421 ccgaattatt ggatgcggca tcaatttccg caatatccac cagtacttcc tcagaagctt
    24481 tggtacatat atcgcattta ttgcaagggt ctccttcatt tggatgcaaa cagttgactg
    24541 cccttgcgaa tatttttgca agagtggttt ttcccgttcc tcttgtaccg gagaaaagat
    24601 atgcatgtgc aatacgaccg gttatcactg ctctttttaa tgtctttgtt atgtgttcct
    24661 gctccactac ctctgaaaat gaagaaggac gccactccct gtataatgct ttatgcgaca
    24721 ttttgtctcc tatccttaaa aaaaccatac acccgtcttt gaacaataat aacaggcgtt
    24781 acatatacag cctgctcggg ccaggcactc ttatggcaca tcggatttcc cgcttatcgc
    24841 tgcttccgct aagacctgac gaggttcaca agctccgatt gcacaagacc cggccgtcat
    24901 aaccgcttat acacggctgc cccactatta gttaccctag gacgggattt ccactctgct
    24961 atagcggatt gcagattaca gggcaccgct aactccccgt ctagtatggc actcaaaatt
    25021 atataatatt taaatgccgc atgcaatcgt aaagaccgaa tccgcaaaac ttaagctctt
    25081 taccgctgtt tgtaaagata aataaagcaa cttgcgtgat tcccggaaaa gctggttgaa
    25141 aaagcgtatt gaacaggtta gaatgtagac aatgaccgga aaaagttacc cgatgcgggt
    25201 acatatcata aatgtaatac atttaaatag aacggattaa gaaaggatat gagatgagta
    25261 ctaatgattt gatggacttt gtttcaaaaa atgtcaacat ctcttcaaat ccgtccgagt
    25321 tgaaaataac ggacatgaga tttgtcgata ttgacggggc accgaagaga aacactctta
    25381 tcaagattat gactaaccag ggtattgtag gatacggaga gataagagac ggttccagta
    25441 aaacctatgc tttgatgctg aaaagtcgca tcttgggcga gaatccctgt aatgtggata
    25501 gaatatttcg caagataaaa caattcggcg gtcactccag acaaggcggc ggtgtttcgg
    25561 gaattgagat tgccctttgg gatttagcag gtaaagctta cggcgttcct ctttatcaaa
    25621 tgctcggcgg taagttccgc gataagatac gaatgtattg tgataccgat gttgaaggaa
    25681 gacatacggg taaggacatg ggtaatgcgc ttaaaaagcg catggagatg gggtttacat
    25741 tcttaaaaat ggatttaggg ataggattgc ttttggatat accgggaact ttgaatgccc
    25801 cgctcggctt tattgaggat atgagaaagt attcttcaca tatcctgaat gttcagggcg
    25861 gttcggtaac aaaggagatg gtgaagcata gaaagagtta tgacattgta aataccgctc
    25921 acccgtttac aggaattcat cttaccgaaa aaggactgga ttatttggaa caatacataa
    25981 aagatgtccg gtcggttatc ggttatgaag taccgcttgc agtggatcat ttcggtcatg
    26041 tctgtgttga ggattgcata aaatttgccc gcagaatgga gccgtataat attgcatgga
    26101 ttgaagatat gattccgtgg atatatacag atcagtatgt aaggctgaaa aattccacca
    26161 ccataccgat atgtacggga gaagatattt atctgaagga aggctttgcc gaattgataa
    26221 aagcaggagc ggtctccata atccaccccg atatattgac ttgcggcgga gccttagaat
    26281 tgaaaaagat tgcggatatt gcggacgaat gcggggttgc cgtagcggtg catatggcgg
    26341 aaagtcctat agcctgcatg gcggcagctc atgtttgtgc agcaatgcga aatgtccttg
    26401 caatggaatt tcactctgtg gatgttccgt ggtggagcga tatagtggta ggtttaccca
    26461 agcccataat acaagacggg tattgcaccg tgccggataa accgggactg ggaatcgatg
    26521 ctttaaatga agaagttata aaagaacata ttaaccctca aataccggga atgtgggaag
    26581 atacttccaa atgggataaa gagttctcga atgacagaat ttggagttag aaaaaacagt
    26641 aatctgcaaa aataacttat agagaggtat aaaaatgcat ttcaacagca aggaagacat
    26701 aattgaaatg actcccctgt ggaagggaga gcgcttagag gacggcagac cttatgttga
    26761 tccgaagtat ttgacagaac taagaaaaat gacattggag gaagtatgga agcctatcta
    26821 tgtcaagggt tatgaaaacc agtttgaggg gcgattaaaa accttgcatg atgacggcag
    26881 aaagcttatc ggcagagcgg tcacctgcac attttgtcct actcgaccgg atttgcatga
    26941 aataatgttt aggataggtt caaacgaagg aagaaaaggt aattacaatc agtgggtaat
    27001 cgacagcctg gttgagggtg atgtagttgt agcggacatg tatgataagg tctataaagg
    27061 tacttttttg ggaggaaacc ttacaacagc tattaaaaac agaacaaaaa ccggaggcgg
    27121 tgttatctgg ggcggcattc gcgatgtgga acagatgaag caggcggaag ggattcaggt
    27181 atattatcgc ggtattgacc ccacacccat acgggaatgc gttatgacag gctttaacac
    27241 tccgaccaga ataggtaatg ccatatgtct tcccggagat attgttttcg gagcgggagg
    27301 aggagtatta ttcattccca gccacttggt tgccgaggtt gtggaaggtg cggctaagac
    27361 gcatatcaaa gatgaattcg gctttgagat gataaagcag aatagattta ctacggctca
    27421 gatagaccgg aatacatgga ctgtggaaat gctggatatg ctggtagact ttatacagaa
    27481 agatgagcgc ggtaagaatt atcgtgatat tgattggtca caagaatatt acatggctaa
    27541 acacggcgat ccgaacgata cgcagacagc attgtagagg tgtttgatga aactacgttg
    27601 gataggacaa agcggatata ttttagagga caataccaca gaaattataa tcgacccgta
    27661 tttatcggat attgtaaata aagttgcaaa tcgtccgaga ttagtgccgc ctcctataca
    27721 accggaagat atttatccag attatgttat ctgtacacac gaccatctgg accatattga
    27781 tcccgagaca attgaaagaa tgaataaaga cactgtttgc tttatagcac cttcttcctg
    27841 caaagaacgt ctttcggctt tggggtgtaa aaacattaaa accatagatg cgggagagca
    27901 gatatcagcc ggaaagtttc agataacggc ggtctttgcc tatcattcgg tagatgccat
    27961 cggaatcata gcggaacagg attcaataaa actgtatttt tccggtgata ccttgtacca
    28021 tgagaaattg gaagagttga aagaatcagg gattaccgtt atgtttgtat gtattaacgg
    28081 aaaactggga aacatgaatg cggacgaggc tgcaaagctg acaaagatta tcaatccttt
    28141 ggttggagta ccgacccatt acggtatgtt tgcaagcaat acggaagatc ctgaaaaata
    28201 cacatcacag attgataacg gctttatcat ggacttcaat aaagaatata ctatttccga
    28261 attattaaga ggagaataaa tatgcaacat aaattaagcg gaaaaagagc actcattacc
    28321 ggttccggtc gcggcatcgg aagagcaatc gctatcgagc ttgctaaaaa cggagcggat
    28381 atcattgtac attatttagg taatgtaaaa agtgcggaac aagttaagaa acagattgag
    28441 gcattaggcg gctcctgttc aatcatacaa gcggatttat gtaaaccgga gacattggaa
    28501 ggatttatcc gaaagatagg cgatgttgat attttagtgt tgaacgcatc ggttcaatac
    28561 agaaaccaat gggaaaacat atcgcttgag gaattttacg atcagattaa ttgcaatctg
    28621 ttagcaagca tgttacttat tcagcaagcg gtaccgtata tgcgtaaaaa acattgggga
    28681 agaataataa cgataggaag tgtacaggaa gcaaaaccgc atccggacat gttggtttat
    28741 tcggcatcca aagccgcgca aaccaatatg gtaaaatcct tggcggttca gttggcaaaa
    28801 gacggaatca ctgttaacaa tgttgcaccg ggtgctatta ataccgaccg caacaaggat
    28861 gttctggaag ataaggaata ttataaaaaa gtgacagatt ccattccggc cggctttatc
    28921 ggtgaacccg ccgactgtgc cggtattgta tctttacttt gttcggaaga aggaaggtac
    28981 atcacaggtc agagtatata tgtagacggc gggaaatgcc tgtaatttga aaggagcaag
    29041 taatgagtca tacattatta aaattttttg aagaacagaa aatgcagagc aacaaaagaa
    29101 tagaaggcga tattcagaaa taccgtaaag cctttgcaaa aataaaggta gttaacagcc
    29161 aaggagagtt aatagaaaac gttaaaggtc gcataaagca aaagacgcat gattttaagt
    29221 tcgggtgtaa tatatttatg ctggatcaat tcccggatga agaacataat aaagcataca
    29281 gaaaagagtt taaaaagctt tttaactata ccgttgcacc gttttattgg caggattttg
    29341 agttgaaaga cggagaaccg agattaggga aaaatccggt taatgtttac cgcagaccgt
    29401 ctattgaaaa gatattgaaa tattgcgggg aaaataatat tgatgtaaaa ggacacccgt
    29461 tattctggca tctgttttta ccccaatggc tcgtgcagga taagaatgaa tcatactttc
    29521 ggatagaacg cagacttcgt gagatagcag aggtatttaa agaccgtatt cgtgattggg
    29581 acgtcgtaaa tgaatcatta tcccgtcctc ctcaccggga aaactatcag cttccctacg
    29641 actatgtcaa ccgcattttt gatttggcgg acagatattt tcccaagaac cgtcttttta
    29701 taaacgatac attaagattt tgctggtcgg aatttatatg ggagttgagc ccttattacc
    29761 tgcagattga gaatatgcta atgaaaggta aaagaattga tgcaatagga ttccagtacc
    29821 atctgttcaa aacacctgaa agattggaaa gagaggcgga gatattgctt aatcctacct
    29881 gtttattcaa tgttatggat acatacggaa aattccataa gccgatgcac gtttcggaag
    29941 taaccgttcc ggcattcggt ctgacagatg agaataaaga ggttcaagca aaattaactg
    30001 aatggttata taaagtctgg ttcagccatc cggatatgga atccattgtt tggtggaact
    30061 tagtggacgg aactgcagca tacgcacctt tgggaagcgc ggaaggagaa aactattacg
    30121 gcggcgggtt attgaattat gatatgagca agaaaccggt ttatcatgct ataaataatc
    30181 tgataaacaa agagtggcac acggatttga aatttaataa gaatgagtgc ttatttaacg
    30241 gatttttcgg agaatatgat atagaagtta ttgcagacga caaaataacc caccataaaa
    30301 tacatttaag taaagacgga agcagagaat ttgtattgac tgtctgatgg ttttatagct
    30361 tttaatattc tctgtgatag tttttcagcg gttacgggtt ccaacaattc ctctgaaata
    30421 tatcttacaa tgcttattaa atccttttta gcattttcag aaaatattat cttaaatttc
    30481 attttcctaa ctctttcttg aactcttctt ccacttcttc caaaccgtat attcttccat
    30541 tttcaaagtc attatgagcc tttaacattt ccatattaaa ttccgcctct gataaaatag
    30601 gcaaagaaag cggtttagaa tcaggtattt tgacgtcaaa aggtaaaccg ttatgcatga
    30661 caacctgtcg caaaaaaatt gatacggcat ttgacattga tattccgagt ttttccagta
    30721 tttcttctgc ctgttctttg atataaggct ctactcttac atatatattt gatgttcttg
    30781 ccatatatgt tgctccccct ttcgcttaca ttatagcaat tgaattgtga ttagcaatac
    30841 attcgcaaac ttttcgtcag tgcttcatgg caagagactg caggcaatcc ctttgcttat
    30901 atgtcatgcc tttaggcagg tattttaggt ttatttggtc tttgttataa aaatgcaaat
    30961 agaagcacaa tgccgcaaga tacctgccat gcggtatgga cagatgaaac ccgtcacgga
    31021 aaagtgatat accttcatag tcgggattga acaggcactc cgtatatgag ttatatatac
    31081 agtctccggt atttataacg agtatattaa actgagcgga aataatcttt accgtcctgt
    31141 ttatgctgtc atacattgtt tttgtattat tattgtaatc ggagaaatat gagtgttggc
    31201 acccgtgagc atacggccag gttttatgta acattatttt tgcatccggg acattctgtc
    31261 ttatatagtt tataagttcg gaaatgtacg gaaaatagct gtttaatata ccgctaagat
    31321 gactgacctg ctgtatcgta ataacatccc attgctcttt atttagggct ttttccaagg
    31381 atatcatgca taatttttta ccgtttttct gatactcata tgctttttca tttgttttaa
    31441 tattatcgca atgcattttt aagtcacatc ccggtataaa gagatttctt acatatatat
    31501 ccgacagcgc tattttttcc atatatacgg tagcatcctg cgagaaactg ttacctattg
    31561 tgagtatttt catccttttc tttatctctc ttcctgttat taaaatatat aatgatatcc
    31621 aaagaaacca tgagtgtgtt tacaacatag aaccaaaata cccagttaat tttgtcggaa
    31681 attatcttac ccgctattgc acataagtag ccaataagta tgaagaataa aaagacaata
    31741 cttttgcctt ttgccgtttt ggattttaaa gatttcgcta tatttaccgg ccaggaaata
    31801 ccgaaacata taatcattgc ggcttctaaa atctgtgcca tatcatacct ctatcacatt
    31861 ctgtgaagcc gccttcagca gtctgttcat caccgcttca cctatgtctt tagtgtcaaa
    31921 atactcgcaa taaatctttt taatccgcat catgtcaaaa agccgcaaac attcaaatat
    31981 gttggatgct atttccgaag gattattaat acttccggca caaagcttat tgtgggtatt
    32041 atatctgtca aatgtttcct tacaacaaag cacaccgtaa tcatcgcctg acattttatt
    32101 gacagcttcc aaaaccttat gagtttcccc ttttactatt accatatccg ccttgggagc
    32161 ataatgctta tacttcattc cgggtgattt gggagtatcg gtatttagcg catatgctgt
    32221 ttccaaatcc tttataacca gtttcagact gtcatacgga acccctcccg gacgcaagat
    32281 aaccggtctg tccgatgtca aatccaaaac ggtggactcc acacctattc tgcatttacc
    32341 tccgttaata atagcgtcca cctttccgga catatcggcc actacatgct caaacattgt
    32401 agggctgggc ttaccggaga tatttgcact gggtgccgct attgcggtat cggaatattc
    32461 tataagcttc aatgctattt cgttgtcggg aactcttata gcaacggtgt caagattagc
    32521 ggtaacgctg tcaggcacaa tacttctttt ttttaatacc accgtcaagg ggccgggcat
    32581 atacagtctg gagagcacgg ttaatgtttt cggaacttcc ttaacatatt tgtccagtac
    32641 cgaaatatca tgtatatgaa gaataagagg attatcgttg ggtcgtcctt ttgcaatgaa
    32701 tatcttttcc acggcactgc tgtttaagcc gtcggcaccc aatccgtaaa cggtttccgt
    32761 gggaaaagcc acagtaccgc ctcttttaat aatatccgca caatatttca ggctgacctc
    32821 atcgggaaca tccatcagtt ttgtattaat cttgttcatt ttgacttaac agctttcttg
    32881 cctgatcctc cgttatcaga gcatccatca attcatccag atctccgttc ataaccgcct
    32941 ctattttata taatgtgagt cctattctgt gatcggttac ccttccctgc gggaagttat
    33001 atgtccttat tctttcactt ctgtcgccgg taccgacctg actctttctg ttctcggcaa
    33061 tctccttatc ctgattctcc tgtgcaatct catacaactt acttctcagt atcttaaaag
    33121 ccttatccct gttcttatgc tgacttcgtt cgtcctgaca ggccactaca ataccggtag
    33181 ggatatgggt cagtcttatc gccgaatcgg tcttatttac atgctgccct cccgctccgg
    33241 aagatctgta tgtatctatc tgaacatcat tcatgtttat ctccacatcc acttcttccg
    33301 cttccggtaa taccgctacg gtaacggtgg aggtgtgtat tctgccctga gactcggttt
    33361 cggggactct ttgtaccctg tgcactccgc tttcatattt gaacctgctg tatgcaccct
    33421 gtcccgttat ggaaaatacc acttccttaa ttcctcctat acctatctcg ttaaggtcca
    33481 atatctcggt cttccatttt ttggcttcgg catacatggt atacattctt aacaaatcgg
    33541 cagcaaaaag tgcggcttca tctcctccgg cgccgcctcg tatctctatt attacgtttt
    33601 ttgtatcatt gggatcgacg ggaagcaaca gtatcttaag ctgctgttcc aaggcaggct
    33661 tcttttcttc cagttgagcc agttcctctt gcagtaattg cttaaaatcg gcatcatcgg
    33721 agttttcttt aagcataaga tagatatcat caatctcttt tgcagtttgt acatactccc
    33781 tgtaaacctt aactatttcc gaaatcccgt tgagttcttt cattaaggct acatatcgct
    33841 cctggtcgga tattacagcc gggtcggtca gttgtctgct tatatcttca tatttaatct
    33901 ctatttcttt taattttcct aacatttcta cctcttaaca ttcatttata ttatacatta
    33961 attacaatta aataaaaaaa catcataaaa acaaagcaac tcataaataa tacattaaca
    34021 tccgtaaatt attacgaact gaaatgcaac aacactgcat cttgctgctt agctacaaat
    34081 ttttaaaata ctcttttatt gcgtccacta ttcttcggga agccttgccg tcaccgtacg
    34141 gattaacggc attactcatc ttgtcgtaca ccgatttatt gtcaaacagc attctgccgt
    34201 cccgtactat atttttatat tccacaccgg tcagttttac cgttcccgcc tgtaccgctt
    34261 cggggcgttc ggtttctctt ctcagcacaa gtacgggcac tcccaatgaa ggtgcttctt
    34321 cctgcaatcc gcccgaatcg gtcataacga aatatgctct tgcgataagg ttatgaagat
    34381 cggtgaatat aaggggtggc agtaacgata cattatctat accttccagt atcctgtttg
    34441 ccgtattctt tactgcggga ttgggatgca ccgtgtatat aaagtgggca tcgggatatt
    34501 ttgatgcaag agtttttatg gcattacata tattttctaa gtttactcct atattttccc
    34561 ttctgtgggc cgttatcggt attattcttt tgttattata atctatcctg ttaagtaacg
    34621 gttctttaaa tgtatagtct ttttttaccg ttgtctgcaa agtatctata accgtatttc
    34681 cggtaatata tattccgtct ttgcttatat tctctttgat aagattgtcc gcattgttga
    34741 tagtagggca gaaatacagt gaggatatgg aatccaccgt tcttctgaca aattcctcgg
    34801 gatacggcga atatatgtca tcggtcctta atcccgcttc cacatgtccg atgggtattt
    34861 ttttataaaa agcggcaaga gatgcggaaa gacaggtatt ggtgtctccg tgaaccaata
    34921 tcatatccgg ttgctccttt gccagaatat cggacaattt catcagtatc gacgaggtga
    34981 catgctccag cgtttgcctt tctatcatta tatccaaatc atatttcggt gttattttaa
    35041 atatatccag tatctcattg accatttctc tatgctgccc cgacacgcat actatatgat
    35101 ctatttcttt ttctttgtcc agaagattca ctaaaggagc catttttatt gtatccggtc
    35161 tggtgccgaa taccgacatt attttaatca ttgctttgtt cctcctccat gacttccgta
    35221 ttgccgtttt tagcagcttg actgagtaac ggcttgtaaa gaatatatac cgataaaccc
    35281 atcagaatga ttatgatggc gaaatataac cagaatctgc tgtactgctg gccggaaagt
    35341 aaaaaagccg ttattccgaa cactatatct atcaaataca gaattagtac ggtttttcgc
    35401 tgtgataatc ccattttcat aagtctgtga tgtatgtggt ccttatcacc ttccgcaatg
    35461 ggttttttca tgattattct tctgaaatat gccaaggata tgtccagtac cggtaatccc
    35521 atggcaacaa gaggaataaa taccgctatg gcggcatgag ccttcattgt accctgaacg
    35581 gataaaaccg caatacaaaa accggtaaag gccgctcccg tttctcctaa atacagtttt
    35641 gcaggattga aattgaaggg taaaaagccc gcggcggcac cgcttaaggc tataaggaga
    35701 atgggaatat attcacctac caaccccgca tccggtcgca caaaggtaac aaagaaaaga
    35761 gtgagactgc atattacggt ggttcccgca gtaagaccgt ccaatccgtc tataatatta
    35821 aatgcattgg tgagcatgaa tatccatgct acggtagcta taaaggatat tatatccggc
    35881 agatgaacaa ggttattatt tgtccccaga ttggtgaaat atgcaatctt ggatcccgat
    35941 atggcaacgg tagcaactgc cgctgcaact tgaaatacca cttttataac aggttttaaa
    36001 tcatatatat catccaatat acccataaca gatataatta ctataccgac cgccaaacct
    36061 attacttcat aggtaacaat aaagttggga ttgaaaagat aattcccgaa taccgaaaga
    36121 ccgaaagaaa taattatggc aataccgccg agaagcggta ccggctcggt atgaacatct
    36181 ctgtttcttc tgggatattt taatattttc ttagcttttg caaactttat cactaaagga
    36241 cacaagccga atgcaatgat aaatgaagct aagaaggtgt atatataact caaattaacc
    36301 tccgtacttt accagctcaa tgcctgcttc ctctaaaagc tcttttgata acgcatcggg
    36361 ataatcccct tcaaatacta ttctcttaat gcctgcgttt ataagcaatt ttgcgcatat
    36421 aacgcacggt tgtatggaaa catatatagt ggaaccgtct atggatattc cggcccttgc
    36481 agcctgtaca atggcattct gctcggcatg tatcgcacgg cataattcat gcttctctcc
    36541 tgacggaaca ttcatttgca tccttacgca tcctgtttct tcgcagtgtt tgcaacctac
    36601 aggtgctccg ttgtaccctg tagccagtat tcttttatcc ttaacaataa ctgcaccgac
    36661 agcccgtctt aaacaggtgg aacgggtttt gacaagatga acaacttgca tgaaatattc
    36721 atcccatgaa ggtctttcca ttacttcgta ccaaacaatc tgtctccggc atcacctaat
    36781 cccggaacga tataagcatc atcattcaat gctttatcaa cacatgcaca gtatatgttt
    36841 acatcgggat ggtcatcatg gactttcttt atgccctcag gtgctgcaat tatgcacata
    36901 agcttaatat tcttgactcc tctttgtttc aaaaatccta tggctgcaga agcggatcct
    36961 ccggttgcaa gcataggatc aagtaagata atcattcttt catgagcatc ttccggaagt
    37021 ttgcaataat actcaaccgg ttgcaatgtc tcatgatctc tgtacaaccc tatatgccct
    37081 atctttgcag tgggaatcaa ggtcaaaaga ccgtcaacca tacccaatcc ggctctcagt
    37141 atagggacaa atcccacact tttacctgat agcaccttta cggtagtttt tgctatcggt
    37201 gtctctacct caacctgctc cagcggcaac tcccgtgtaa cctcatatcc catcaatgtg
    37261 gatacttcgg ttacaagctc tctgaattcc ttagtatttg tattcttgtc cctcataatg
    37321 gatatcttat gttgtataag agggtgatcg aatacgaatg ccatgtccat ataattggtt
    37381 ccggacatac ttcctccttc ttctatcctg cggatcaatc cgttccttaa ctcatatttt
    37441 ccttcaaggt aattttcgtt aagccaaatg tcgataatct tgcatgccat atctttcgga
    37501 gtaattctgc ctgcaagcac cagtatgttg gcattattgt ggcgctttgc catacttgcc
    37561 tgatattcgt ccatacacaa tgccgccctt atacccttga acttgtttgc cgcaatgctc
    37621 atacctattc cggttccgca tattaaaatg cctctttcgc actcgttctt ctgaacggct
    37681 gatgctacta aagaggcata aaacgggaaa tgacaggaat cttcgctgta agatccgaaa
    37741 tcccgatacg gtatgttctt ttcctcaaga tacaggagta tctcgctttt cagcttaaaa
    37801 cccgcatgat cacaacctat ggctaacatg ggatatctcc tctgttttta tttaaaagaa
    37861 ttctatcaca aataataaca tatatccaca ctaaattaaa tttatcaaat taccaaactc
    37921 ctgataatat ggtcgtacga acttagattt gtttatttac atttatcaac ttattaatat
    37981 atattcatcc tctccgaaag aaactatatc ccctctttta actttaacaa aactttccga
    38041 cttaatccta tcaccgttta agtaagtgcc gtatttgctg ccgagatccc gtacatatac
    38101 ggtatcatgg gaatatctca tacgggcatg tcttaaggaa gcaagtttgc ccggaagtcg
    38161 gtaatcacaa atcaacttga gtcttcccac cgttatgtct ttatcggcaa ttattatgca
    38221 gtcgtcggta tctactatgt ttttaaatac ggctttttta ggttcttcac aaactttctc
    38281 ctgcacatca atatttccgt cataggacgc ctttattatc ccgtttatat caaaatgctc
    38341 agaagataaa gcttctttaa tacttctatc aatataatta ccttcggtta tttcgcttac
    38401 aagctgttta agaccgttat ttaagtcgta cttattgaaa ggatccaata tgtatttgaa
    38461 tgtttcatcc ttgaatacat atatataacg tgtatccgga actacatatt cggcatctat
    38521 gagataggag cctgttatcc tcatcatatc ctttatactg tataaaagct tttttaaaag
    38581 agcgggagaa tatcctttct ttttcagata taccgataaa cttgttccgt taagcgtgtc
    38641 gaatatcagt tcatcaccct tattgtattt caaaggtagt aagcaatcga taaagcaatg
    38701 ttgcagcatc tgtatcatat ggtcgggaca caatattttg tcaccgctga caagcaacat
    38761 cctgtcttgg ttgctctgta ttatctcgct tatcatttca tttaaccctt aacttttcca
    38821 aaatactctg attattcagt gtaccgtcaa ttatcccctg cacaaagttt gttatggctt
    38881 cccggaagat taacgctata cctaccagaa cggcaagtat tattatcact tccaccgtac
    38941 ccattccttt ttttccgttt tctattctca tattttcctc cttcacctat atggtagtga
    39001 ttttatggtt atcctatata ctcataagca taatcatcgc aggcgcggta agaaccaaca
    39061 gtattcctat cagccctatt gccagagcca ataccgcttt tgcagacgat tgctcagctc
    39121 tttttttggc attgtttctt cgctccgtcc atatggatga agtatatgtt tttagagtat
    39181 cttttattcc cgcttcggta ttcttgaaac cggttatcat tatggaacaa aaagcattaa
    39241 tctgcggtat gggaagcata agggataatt ccagaaagga gtattccggg tcggaatgca
    39301 ttgtaagatt gtttatgagg cggtccagat gcaccttgaa agttctatcc ggaaggtcat
    39361 aatcctgtcg ggtaaaagcc tgataaagag tcatacccgc tcctaaacac aatgtcaact
    39421 tggtcaggaa taccgaaaaa tccgtaataa tctcctgctt tttcttttca tacactttag
    39481 atatgtcgaa atccacacac tttacacttg caaaacaaag taataccgat atagcggtaa
    39541 ccacggcaga tgctttacat gctattgcag ttgccggaag aaaaagcatg ataacgaata
    39601 tgttcatttt taaacagata tcggtaatat gtaaatcttc ttttgctgtc ttgggatata
    39661 gcaaatccag tcggttatcc gaagcggtaa atctcgtaac gacttttgcc aacggaacaa
    39721 aagccttata tgtcgccttc atgaaagtac aatgacacaa aggaaaatca aaccgcactt
    39781 tcctggaata aagcaaggta aagagtatta ataaaatata aatagcaaaa aatatcatac
    39841 ctttacctcc agtatctttt ttgcaagaca tgaagatatt aagaacagta tgcccgccgc
    39901 agtcattatt gctctaccca aaaaaccttc ataaagaata tccagatatc cggtacccat
    39961 ggcattgaaa agagcaagta taaggaaggg acagagactc actataagcc gctcggtttt
    40021 cttttcttcg gtgattactt ctatctcctg ctcaatactt cttttttctt ttataaaagc
    40081 aatggtctct tttatagtcc tgttcaaatc cgcacctttc ttctcacaaa taatgatgct
    40141 gtcacaaaac aggtatgctt cctcaatatc catatgctct gcaaacgatt gcaaaatact
    40201 gtcggtagac atatttgcca ttacttttga ataaatgttg tcgattaatg taagcatata
    40261 atctttagga tatattcttt tgaactccag aagcgctttg gaaaacgcat gaataaaatc
    40321 cgatggtatg gcagtgtaag acagcagaat ctccagaaat cttaaaaaac cgtctctgtg
    40381 cctcttttga accgtttctt tcatgtattt tttatcaaga agacatattc ccgcaacaag
    40441 cactcccgtt attattgaga caataaggga aagataaaga aaatagacgc atattgcata
    40501 tacaagaccg gttgttaccg ccctctttat aacctcaaac ttcatctccg tctccttgtt
    40561 gcttccccga ctttgatatg cttccccgat tcaatatcat aggtaaacaa gcactcggtt
    40621 ttaatctcac cgtcttcaaa ctccgtaacc tgtacaattt cggtaagcac tctctttccg
    40681 tcaatccttt gcagtgaaat aaccatgtcg atggcatcat aaatctgctt tcgtatagcc
    40741 aaaaaaggca tatcacattc catcaacacc atggtttcta tgcgggaaaa tgcatcttta
    40801 caggaatttg catgaccggt cgatatggaa cctatatgac cggtattcat agcctgcagc
    40861 atgtataaag cttctttacc tcttatctct cccacaataa tcctgtccgg tctcattctt
    40921 aaactggcac gtatcagact gtccatgctt atctcgccct ttccctcatt gttgggattg
    40981 cggcatgtta agcgaattat gttgtctata cggtcaatca taagttctac cgaatcctca
    41041 attgtgataa ctctttcact attgggaaca aatccggtca atacgttcaa taatgtcgtc
    41101 ttaccgcatc cggctccccc gcatataaat atgttcagcc gctcttttac cgcattgtcc
    41161 agaaaatccg atatctcttt tgtaaagaaa ccgtctctta tcatatcctc ataagttatc
    41221 tgcttttccg gaaatcttct tacggttata ctgtggccgt tatgggagat aggctccaaa
    41281 atgatattca cccgtatatc atgccctata ctgccgtcca atatgggatt ggccatgttt
    41341 atggttctgt cggtctttct gcacagtatc gatatcagtt tgtctatatc ctgttttgta
    41401 aaactgatat catatcgatg aataataccc ttttcctcgt aaaatattct gtcggcgttt
    41461 accattatct cggtaatacc ttctttttcc accaagggct gtaatatccc gtatcccgtt
    41521 atggcatcca ttaccctttt tatgacttcc ggcttattct ccatatcttt attctttata
    41581 tcatacacat actcggtaac agttttttca atttccccct cctccatacc ggctatgcct
    41641 tctaaacact gtttaatgat tttgtcataa tcgttattca tcatttttct ccagttcata
    41701 cacaacctgt tcgattgcct catagaaatc gtacggatta tgagttatat cttcttcggc
    41761 tttgggtata tatattcctt cggtataccg gatcatattg cataactgca taacccttat
    41821 atcattcttt tgcaaaaaag ctgcaacctc ttcttccctt tcactgcccc tgcatttaat
    41881 aaacagtgtc ttgtatgcat ttttcatgat aaaggcatac ggactgattg gatataacgg
    41941 gcagtcgatt atagtgtagt tatatatact gtttgtattg agactgttaa tgaacacttt
    42001 tgcgctgttc atatcaaaag aaacatcata tgaaggatat atactgttca gaaaatacac
    42061 gttttttgca acatcatggt ttttgtacct ttcaatacac gtattgatat tctcgttacc
    42121 cttagaagca tagtatgcta tgagcgaaat atcattctgc tgttcacatc tgaaaagcga
    42181 atcttttacg cataatccgt caagattgat atacagtacc ttcctgccgt ttctggataa
    42241 atatattgat agagcttgtg aaatgaatgt cttacccgct ccgccatgag ccgatgttac
    42301 aaatatcgcc ctgttctccg actttacatt aataccggcg gatatatcaa tattctccat
    42361 gattgcactg attatcttat ctgcggactg atacttacct gtatgccatt ctttagagga
    42421 tgtcttcaaa agaagctcat tctctgcacc ggaatcttca agaacaacat ccgcatgttc
    42481 ttcagtcaga gcgaacacat ttaacccgtt aatcttaatg taatctttca gccgtccggc
    42541 atattcatta tccgacaact taacatgtag ttttatctta ttcatattaa ctcctgccgg
    42601 ttaattatac cattatttac catttattct cattcaacat atgacaaaca catgtaacat
    42661 aatataaata gacagtcatt ctttatttat gactctgttt atgttgataa agcaaataaa
    42721 gaaaactctg ttttcctgaa atcggaggga taaaactgaa tcataaggca taggctgctt
    42781 ttcaatgagt agctgaaaaa aataggtatt tcatatgaag gaggtatgca aacaccggta
    42841 aataaagtta gaaaagaatc aacatacctc ctgcgcctct tccatgtccg gagctgtagc
    42901 ggattccttt actattcctt ctaaaacctt aaactgaaag tttttcgatt taaatgctgt
    42961 gaagttaacc ctgttttcaa tccttacaac tacaccttct ctgatgtgtg ttttaccgat
    43021 agggtcttgc ccttcctcat aaacatgtac atggtccagt aaatcctgtt ccgttgtgta
    43081 aataaacctg tcaaatagcg gcacaacatt taatcccatc tgttcacaac gcgcttgtat
    43141 caggtgataa ggatattcaa cggtttcgcc gtcagtattg gtcaaagtca tacggtaaac
    43201 atacatctcg gaagttgccg gctcacaacc gtagtggaat acggtttcat caccgtattt
    43261 tttagaaaat tctttatcct gcaatttaga gtttttacaa gcgggcatta tagtggtaga
    43321 ttggtttaca tatcctacta tctcataata gacagtctct cccttatgca gtttgccttc
    43381 aagtttatcc gcatgaatct ttctgaattc ctcatcaaaa cttcccaaaa caactctgcg
    43441 tgtacctgtg atgtaggtgt actcctcata tgcattaccc tgctttttga atattttgtg
    43501 caaaatattt ttcctgtatt tcttcacagg aagatagcct gttctttgtg atgttccgtg
    43561 cattttaaga gttatctcca caatatcacc tgctcggaaa gcatctgaat taaacgcaag
    43621 ctgttcggta tctttatgtt ccttaaattg cggacaaatg atttccttaa caataccgga
    43681 ctttttaaca cctgactgtt tttgaccgct tcttttagga atgtattttg tgcagataag
    43741 atgtccgtta aattcgtcga ttgtatcacc gtacttcagt tgcgttatat cgccgaacgg
    43801 ttccaatgaa gaaagcggca ttaccaaccc gtccgattta gtgcctctta atgagattgc
    43861 cttaatgttt ctcttttcgg gatccagata tccgcctatt gaattgccct ctgcgtcttt
    43921 cttccttaaa agattattag tttctgcata ctccatactc aactgtccgt ctgtcggaaa
    43981 atagatgtat agggaagttt cgtttacatc tttaccgata acaacgggat ttccgaaaca
    44041 gaacccctta ttcaatctgt ctgcgttttc tatcggctgt atgtctttta ctcttgtaac
    44101 atatgctttg taagtcattt tacatatatc ctcctctgat ttcatttctc tttgttttaa
    44161 caacaatacg ttcaatgcgt ccggcttcac gcagtttctt tatctcacac tcaactcgat
    44221 attattactc tcccgcattt caaaatcact gtcttgtaat tcgcttcttt ttgccataat
    44281 atcagaaaaa ttatcaatac ttaatagaca attcggaata ttatcataca aaacaggtaa
    44341 tatggattaa tgaaaatatc aggaaccctt tggatagtaa tattctcgct tgaacttaaa
    44401 aaaactaccc gtttccggta gttgtgctaa ttgtaaaact tctttattta taaaaaaaat
    44461 ataaaatgct caccgctgct ctctcaccca ctgatatgcc aacctgggcg ttccgtccgc
    44521 aataattatt atcccgcagt aagtgaatcc gtttctctca atcgctctga tcatcggcac
    44581 attcaatcgg tgtgtatcta cacgcacact gtcagcgcgc tccttacacc aggcaaacgc
    44641 ggcatcggaa ataccgctct ttctgcctct gctggcaatc ctgtgaatag ttatatatga
    44701 ctgtttatcg tcaatccact caccgtcaat cttatcatac gtcggttccg gaaagttctg
    44761 tgcgaaaaat acaccttcaa tttcgccggc atccggtgtt cctttcctgt ttaccagcac
    44821 catacaaaga cctgttcgga tatccctctc tatatcctcc aaaagcgggt aagccttcca
    44881 ttggtccgca ttaccttgtt ttctcatata tgcccgtgct cctcgaaaga tatcatcaag
    44941 tatctctaaa tcatcgtata ttgccggacg aatgtcgtaa tgagtcatag cagctcctgt
    45001 tttttggcgc aactataata tcccacactt ttatgtagaa tattaacagt taatctatct
    45061 ttttgcctta ggtttaccta atatttcact taaaactatg ccttttacca gggtttcact
    45121 gtcttccagc aaatccccta taaaatcata atccttttta tccgaaacct gttccgtaac
    45181 cgttgaagcg gtttctttcc gctgcaactt ttccatatcg tcatctgacc tgtaatactc
    45241 acccattgct atttatcttt cttaatagcc tgaggtttag cggcttttcc ggcttcggat
    45301 atggattccc tcatcttggt atccgccatt acatttctca tattgtaata gtctaaaact
    45361 cccaagttgc cttcttcaaa agcttttgcc atagctttag ggataagagc ttcggcttcc
    45421 acaacttttg ctctcatttc ctcaatattg gctttcatct cctgctctct tgctacagcc
    45481 attactcttc tttcctcagc cttggcctgt gcaatcttct tgtccgcttc cgcctgatcg
    45541 gtctgcagtt gagcacctat atttcttccg atatccacat cggctatatc aatagacaga
    45601 atctcaaatg cggtacctgc atccagtcct tttttcagaa cggtctggga aataagatcg
    45661 ggattttcca atacatcctt gtaggattcg gatgagccta cggaggttac gattccttca
    45721 cctactcttg ctatgatagt ctgctctccc gctcctccta tcaatcttga catattggca
    45781 cggacggtta tttttgtttt ggcttttact tcgataccgt cttttgctac agcggatatt
    45841 ataggggttt caataactct ggggttaaca ctcattttaa ccgcttccaa aacatctctg
    45901 cccgccagat caatagcagc agccgtttca aaagacaact gtatacgcgc tttctgtgca
    45961 gcaatcaaag catcggcaac tttgtttaca tttccgcctg cgaggtagtg agtttccaac
    46021 tgatttattg tcacattcag tccggccttg gtggatttta taagggggtt tactatctta
    46081 gccggttgaa cccttcgaaa tttcattcct ataaggctcg tcaaactcac ttttacgcct
    46141 gcagctatcg ccgatatcca caatccgata gggaccaaac tcaacaatgc cgaaaagaac
    46201 gctattatta ctgctacaat aatcaatact acaattaacg gatccattaa acactctcct
    46261 ttatttcttt tactactatt ctgttacctt caacctgttc tatcgttact tcttttcctt
    46321 cttctacaaa ctcaccttta gtgacaacat ccaatatttc tccgtcaaac accgccttcc
    46381 ccgcaggtct taatattgtg actgctttgc ctttaagacc tatcttggcg ctcatgtcct
    46441 gtaccccgag gtaaccttgc tcttcggaaa aagtgtcggt attgataagt ttcttggaaa
    46501 gcctgctttt tgttgcaatc cttattacga taaacagaag tattaccgct attaccagaa
    46561 ggataagaaa cattatgaca ccttcaagga aggtttttgc ggtaagaaaa attgacaata
    46621 aaagcaacac gattcctata ataccgacta caccgaaact cggtataaaa gcctctatga
    46681 tgagaagtat catgcccaca acaagtaaga ttgcttgcag aatactaatg ttctcgacaa
    46741 aactgaaaat acccggcatt tttatctctc cttccatatt taattatacc ataccgtttt
    46801 ctatatacgg gtattatctt tgcaacttcc tcaaatccga tccgtccggg ttcatggaat
    46861 aaatatatac atcggttaat tgtgtttcgg gaatccattt gaaatccaat accataaccc
    46921 ttccgccgac tacatttaag tacattccgt aagaagcacc ggaatagata atcttctcat
    46981 ccgaaccgtc aaaattcata ctgaccaaat catacgacca ggaccatgta gaacgtttga
    47041 aatccggatc atatgctata tagtaaattc ttccgttata tgcattcatg ccgaacattt
    47101 ttttactttc ttcggtcttt atacacttat tattgttgcc gtcaaggtcc atagtgcata
    47161 tgtagttgta atattggttc atatagacaa ttttaccgtt acttataagg aaaatatcgg
    47221 catccgcctg taaaataaca tccttatttg tcccgtcagg atcacattta atcatgttcc
    47281 cctgattatc tatgaaatag agataattct tgtgtccggt ggcaccttca gcccatctgt
    47341 aaatctgttc ctctgcggaa ccgtcaggtt tggacctgta taagtcgaat gaatgcgagt
    47401 attcaacatt tcttttaaaa tagtacagat aatcaccgaa cttaaaaaac cctttacagt
    47461 attcacctac tattgtttcc agaccgcttc cgtcaagatt catacggaac atattgtatg
    47521 tgtcgtccac ggtatctccc tgatttccgc aaaaatacag tttgttatca ataaccaaca
    47581 aagaccatcc gaatccgtca aatattgaca taaagttttt gccgtccctg tctacacgat
    47641 aaatatgcgc cttactattc tcatcatagc agctatagaa gatgtagtct tccgttcgga
    47701 aataatacgg actgttggca atattaccca gttcatcggc agattctccc tccgatacga
    47761 ccgcggaaac tgtatcggtg ccgggacccg gatcgggacc ggggtcggca atacgacctg
    47821 ctgaagcagt cggttttgca acattgttgc cgttcggctt tccattcctc aataaattcc
    47881 gtattaacaa acctgcacct gcaagaattc ctataacaac aagtaccaca attatcgtaa
    47941 ccgcaacgga tgcttttttc ttgggttttg cggaaggcat cctctgattg acattcggtt
    48001 gcgcatacac aggtggcgga gcaccctgca caaaaggtct ttgctgtgct gtcgtctgaa
    48061 caaaaggttt cggttgaggt atcacaaccg gcgccttgga atccgcaccg cagtttgaac
    48121 agaatcttgt acccgcaggc attttggtcc cgcaattggt gcaaaaagtt gctgcaggtg
    48181 tgcttgcggg tattatcgga tatttagcaa gtcgctcatc atccgttctt ttgaaattcg
    48241 cccccaaaga tacggctatt cccgcaaggg tcgttaaaat gatgcccaac ggtaataaag
    48301 attggtcgtc atcttcattt tttaccacca cttcgccgta tgaaggatcc tttgaaatat
    48361 ccaccccatt taacattgct ctttcatatg cataaacagt attatccttc tgattgaaga
    48421 agaaaaaggc tgcatagagt attgcagcgg agtcatcgtc ttctttctta aatgttccgg
    48481 atatagctat atctgccgaa tcttgaaccc tgtatggaac gtttatctcg ttaagccgtg
    48541 ctgtagcctg attgataaaa ctatccctat tcatttttac ctccggtata aataactaat
    48601 taatacagtt tcattttaac atcattatgt tatatataca atcattttta caacaacaca
    48661 taactgcata acctgcagta attaatcgca atatgtatta cttcagccca cactgtaact
    48721 aagaggcaaa atcgttaaaa taaccctgaa taagtactac cggtgttcct ctgtctccgc
    48781 ttccgctggt aagatcgcat aatgatccca ataggtcggt aatccttctg ggagtggtgc
    48841 ccaatgagcc catgctttcg gtattgtcat tgtccttggc tttaattctt tctttcatag
    48901 ccgaaataag gtcttcaccg cttaacgagg caaattcatt gtccgcaaaa tatttcattt
    48961 tcagctcatg cggggtgccc agaagtcccg aagtgaaacc gggagacact accggatcgg
    49021 caaactccca tattccgcct acaggatctt taaagcaacc gtcaccgtaa accattacct
    49081 ccacatgctt tccggttaaa tccgacattc ttttttgcag ttgttccaca aaagcctgag
    49141 catccctcgg gaaaagcttt acactgtccc ccgtggcatt attggaacct aacagtccgt
    49201 attcctcgtt atagcccgat ccctgtacgc tcgatgtcag tatctcatcc aacctgtata
    49261 ccttttccgc tccggctttt cttaatattt tttgcgtgcg ctttcttgaa tgaatatcac
    49321 aattcagaac atttttcgta tagttcagaa tatatgcggg attattggaa aatattattt
    49381 ccacatgctt tcctgtactc ttgtacactt ctatgtagtc cactttggta aatatatgag
    49441 cggtattctt aaacagtttt ctgaactcga cctcgttaaa actgtctgta taggggtcaa
    49501 cttttgattc gtcaagatca tccagagtga taagggaatt acctacttca tcagccggat
    49561 aggacagttg cacatacaat ttttcacagg acatggcaat agccttcaat aataacaaaa
    49621 acctgttacg actcaaaata ggaaacacta ttcctaaatc cttccccccg aatttacttc
    49681 tgatgtcggc ggcaatctgc tcacatgtgg cataattgcc ctgtgttctt gctaaaacag
    49741 cttccgttac ccctataata tccttatcct gaatggaaaa attctcattt ttcgcagctt
    49801 ctattacact gtccgcaatc atcgatacca gattgtctcc ctgtttaaat atgggagtaa
    49861 tgattcctct ggatgtaaca cctgtatacc tcattatccg tacttccttc cctcaaagtg
    49921 tccgtataga caacatactg aattttaaaa tatacatata taaatgtcaa ataacttctt
    49981 gtttttaatg agttaaaaaa ccgctatatt atatatctca taggtgacag agcagcggcg
    50041 ataagcagat atttcaatgc cgatctcggt atcttgcatt tgacttcgta atgtatatcg
    50101 gaacaggaaa aagtcagtgt cgtcattgta attatagcaa tatccgtaat atccgataga
    50161 tttataacca gctctttttt attcttttta tccttaaata caaatctgtc ggtatacaga
    50221 gccattgaac cgctcagaac atgctcggtt ttttcgtctt cggtatatgt aaacagctcc
    50281 tgattgtcat ccgatgtaat tactttatcg ggattcttct tcattctttc tatattatct
    50341 atgacccact gcctttgcca attatcccat tccagaacgc ttgtatacgg aaatcccgca
    50401 ttattttcgt cattcagtct gcaatgggta tcgtatcgca gtctgacacc gcatttacag
    50461 gaaaatatat cgtcttctga tgtatatgtg cttataccgt gacatttagg gcaaacaaac
    50521 agcaccgttt ccagattttg tgcaagtgtt tttcccttgt attcatattt ttccgtcatg
    50581 tcaatggagt taacatataa gtctttgcac attgcttcat aaatctcgtc cacagtcatt
    50641 ttttccaaca tttccggttc ataaatattg cctatggtac ctatcatctt gcccggtctt
    50701 ttggattttg accatctggg cattgaggga taaccgcctt ccacacgata tgttatcatc
    50761 gcaacgccgg ctcttttaat aagttttgcg gtagattgta taatggttgc cgtttcgccg
    50821 ttccatgtac aactgccttc gggaaacatg cagatattat gaccggcttt taaagctctc
    50881 agcaaactga ccgctgttct ggcctcggtt tttgctttca ttctctttat cggattcaag
    50941 aatgtgcgta agagccaggt aatccagccc attcggaaaa gctgttctcc cgctacaaaa
    51001 tacatatgtt gcttaaagga taatcccaag ataatactgt caagattagt ggtgtggttg
    51061 gctaaagcta taaaatttcc ttttccgtaa tcaacttttt caaactcgta gccatacttt
    51121 tttttcatat acggctcaaa tatacatctg gcaaattcaa ttaccgctct gtgaagcctt
    51181 gttccgctca taatattctc cctatatatt ttacatttac tcaggtctgt ttacaacaga
    51241 aaacggatta atagtaaaat tttaaatata tgtgactaaa atgccagata gaacatatac
    51301 aatcccataa gcagtataag tgctcccatg actatagata taaccttgct tgcttttccg
    51361 taacgctcgc ttgtagaaag tttcttaaca aaacccaccg atgttccggc tataaccgac
    51421 agtacaccgt gcccgattga ataaagcatg aaaagaaaag caccccacag taagtcattc
    51481 tgtgtggcca caatagcaat caatgctacc aataccggtg tggcacaggg agaggaaaaa
    51541 acaccggcaa gcataccggc tataaaagca cctatgccgc ctttttttgt acttttggat
    51601 aaaagattgc tttgcggtat gaattgaaat atctcccatg tctgtaaagc catcagcacc
    51661 attagaatac ccagtatgat ataccaccag gagccggcag ttcccattaa tctgcccgcc
    51721 aaagcagcaa taatacccag tgcggtaaaa gtaacagcgg tgccggcgac atatattaac
    51781 gatagtttaa aagctctttt tgaatcattc tgacccgttc ctcccacata acctatcacc
    51841 aaaggtattg aagataacga acacggcata aaagaagtaa ctacccccgc aagcagagcc
    51901 gataacggtg tcagccaact tttttcggta agcagcggag ctattttatc cagtaattcc
    51961 gtcattcctt tacccccata tctcgaagaa tggaaagaaa ctggtctttt gtcagcactc
    52021 cctcatgaac agtgtatgca tgctcaccgg tttctctcaa attatatgtt ataaactcca
    52081 catctatgtt tttgctcgga acatagggtg tgccgtcggc attgatgaat atttgcgtag
    52141 gaattacctg aaccgggtat acgcttgatt cttttgaata tttgtacaca tctatatact
    52201 ttattataac tcttccgtac atttccgcat gtacggtttt caatgtggga taaaaactta
    52261 cgcacggcag acaggagtcg gaaccgaaat ccaagactat aggcaggtta tatgacttcc
    52321 atgcctccat atccagttcc gatgcgttaa gtgcaaagtg ttcttctttg acaggttgca
    52381 ccaaaggagc attaagtgct atatcacttt tcctgtcata attcttccaa aaccatatgc
    52441 ccgctatggc tacaactatg actacaggaa ttactatttt taatattatc tttttatcag
    52501 gtttcttcat cacatttctc catacataaa tttaaccgac aaatagagta tacaccataa
    52561 atattatacc tactgtgaca ttggcacata aaagaacaga gaagtgatta aaaacactgc
    52621 gattatagcg gaagaaatgc tgaataaagc tgcaaattga ctctttgcag ctttactcac
    52681 gagtagtata ttcaaaagga cacgggtaat tgcttattgt gtcatattaa cggtaacgca
    52741 tttatttgca ccgtcatttg ctataagcag gtacggtata ccgtttacag gctcggttat
    52801 cagattaagg ttaccgtcat aagtgtatac acccaccgca ttagctgagg tagaggataa
    52861 accgtatccg ccggaaggct tgacaaggta catcccttca ggtgtcataa gataaccgtc
    52921 cgttacattc caattatact taaccgtagt ggacagcaca ggtttatccg ccggtaatac
    52981 atcggcaact gccgattcgg acacggcaag ataatatttg tatatttcac tttcatgatt
    53041 aaccgtggcg gagaataact tgtattcacc gtcaagatta catacccata acctgtcatc
    53101 actacctgta aatgcggctg catccaaacc gttacagtaa acttggaaat ccgcctttct
    53161 ggtcagtctt tgcaacgaac tgttgttatt tacatgcaat ataccgggtg ctatcggggc
    53221 ggtcgtgtaa gctgtgaagt ttacactgga agtatcatca acatccgact tggtacggtt
    53281 tacatttaca tacagataat cggtaggatt gtcagcggtt cctcttatcc attgaggatg
    53341 tatattgctc tggaaccatg aatcatgtcc tacatataca aggtctccta ttgcttcatt
    53401 aggcaccaat ccgcttaccg aagtttcaat atcaccgtaa cagaaatcta tcgtaaactg
    53461 gtcattgaat cccacaccgg atgtctcacc ttcttcgttt aacgctctgt tgtccactgc
    53521 cagatatgta aaggctaccg aaccgtatgt cggtcctaaa taatatactc cgggttgggt
    53581 aataacaaag gtatgagcga tgaagtagtt ttctccgtat gcggcatcac ttaatgtgta
    53641 gtcaaattcg tcaactcttg catacgatgc cccgcttgct tccgatccgt atatgttcgg
    53701 aagaggaatt gcggcatacg gtgtgtttct gttggttata aaagtatgcg cataattgct
    53761 tgcctcatcc aaaccgtctc cgtaagtttt aaccggtgtt gcttgctgcg tctctaagtc
    53821 aatacttgcg gttttccaca aacaaagata tctgagatat tcgggatcac acaaaatatt
    53881 atcaagttcg gtagtggcta taacattcac aaagaccgga ttactctcac ttgcctgatc
    53941 aatgacaaac gatgccgtac ctgcaggtgc ataatacgga atacttacgg gattaccgtc
    54001 atattcggta gaatgagtca tcgggatgaa ttcatttttc atctccgtca catcggttgt
    54061 ggtatcaatt ccatcacaga agaataccga atcttcggca acggaaagta tctctacccc
    54121 gtcatataaa gtccagccgg gaacgcttcc tatttcgccg tcatagttta ttccatatct
    54181 cttcaaatcg ggcatatggg tctttttaaa atcaaaatgg gtgaaatcca ccgctcctcc
    54241 gattgtggta agaggttgcg tgtaatagtt atattgcaat tctcctcccg gaagacctac
    54301 ctccacacct acctgaccca atagatacag attgataggt acggtagaag ctgttgaaga
    54361 aagagtaaaa ccgttattaa gatcaaatga caaatagtgt gagccgttat agagttgcct
    54421 gttttcaatg tcgtatataa agtcggcagt ctgtgaagat aagtagtaac cttcaaattt
    54481 ggtattttca aaattaagcg ctatggaagg ttcggatatc tgcttccaaa tatactcact
    54541 gtttaccgat atacagtaat taccgtctac atcaatactg aaattgccgc tgaaatacgg
    54601 attgacgtca acatatgata aattcagcaa ttcggaaagt gtcatagtat agtactgatg
    54661 tcccacaaca gcggctatga gatattcccc tgttcttaaa gtctgccctt ccgattgcgt
    54721 ttcggtgtac ttatatatat cggcctgcga ttccactaat ttgataggga acgataatat
    54781 tgtctgacca tcttccgcaa cttcaataac ataagaatgg aaagcaggct gcacaggtgt
    54841 catggaggct acctgataga gctgaactct tcccgacatg cttgctgcgg ttgaaacatc
    54901 ggtagtaagc ttaaactgaa gattatccga atcaaagtac atataatata tcgtgtctat
    54961 acctgttgaa gtgtcaacat atcccaatag atatacactg tttagtccca aagtatgtgt
    55021 gtaaacacgc catttatatt ccgttaagtc aacggaagac tgatcgtcca caagttcaat
    55081 ctgcggtgtc atgtcctcta aattaccctg tgctcctgtc agtaaatatg tgtcggtata
    55141 tccgcggttt ttcagtataa cctccaaatc ggttccctga tttatccata cgctggaatc
    55201 aaagaaagtc acataactgc tgctgaaacc taaagacaga ttcataagtg ccgtaacgtc
    55261 gatggctctg gaattgtata tgtcattcat ggataaagaa tagtatctgt ccacaaccga
    55321 gtcaccgttc tcgtccactt cggcaactat catataatta cccgattcaa acaggctgac
    55381 cgactgtgta agctttgctt taccggaaag catggaaatt gtatcggtaa catcatccag
    55441 ttccgcactt ttatccacac ggaatatata caacggtgtc gatgtagtat cagttgcggt
    55501 aaatgatgcg gcaacattat tatatgtata tgtccacgtt gcaccttggg aagtacccag
    55561 ataaagtcct tgctggttcc ttcttatgta atatgtctga ctgcctaccg tctttttaaa
    55621 actcgttccg tcggctgtcc agactatatc gggaattacg cttccttcaa gtactgtcga
    55681 acctaaatta tatcctatta ttccgtttga tgtatatctt accgcataac atataccgtc
    55741 ctgatatatt gttatcacat atgtgcccga tgcaggcata ccgctcgaag tcttactcgt
    55801 taaagtgtat gtttcgtcac cgttgtcggt agcctgatac agtgtgacag gcataacagt
    55861 agatgttgtc tctatattat tggtttcacc taccatgaga tgagccgcag caagattggt
    55921 gctgataata ctgtacaacc tggaatcgga acccaatata ccgctatagt tatactccca
    55981 ttgtgtagtc tgagtacctg ttaacataaa gtagttgttg ctgacagtta aataattatt
    56041 actggagcct ccgctgaaat agagatattt gccgataggt ccatctattg caaatatatg
    56101 gtgcacagaa gaagttatat tactcgtcca atcaaccggc aatgtggtac ctaaggatgt
    56161 acgggtaagc atgaaagaat cgtgtcctat cagataatat tcagattggt cataaagaac
    56221 cataacatag gttttcccgt tctgtaagcc gttttcggca agagtatata ctcccgaatt
    56281 atgctcatac atatatacgg tgataccatc ggatggagat gtattaaata cctgcgtagc
    56341 atacggcgta taaacggtgc cttttgaata atagaagtac ttatttgaat ccagataaaa
    56401 tgtagttcct gaactactgt cactgccaaa agaagtgccg ctataagtaa gatacctatt
    56461 ggtgcttgag tttctcaatg ttattcccga accggatgta gcagaactaa aggtataggt
    56521 gttcgacaag ccggtagttg tagtaattgt tccgttcggc tgtaccgata cgccactaag
    56581 ttcggtaaat gaaaacgaag ttccccacca attagaataa ctcataccta ctgcataata
    56641 tgttgttccc gttttcacaa gcaacacttt attgcttcct gtagatgttg taggtgtaag
    56701 agtaccggta ctgctgttat aatcatacag tcttgccgaa tatgacccgg tatcggtggt
    56761 catacgaaga cgttcccctc caaaccaacc ggaagtatat tgacgatacg ggaaattgta
    56821 tgttgcataa cttaactgac cggatgcact cacactccag gtgccgtttg cagttgttgc
    56881 ggaagtaata accccgcttg agttggcgga aatatatcga cctgcatttg ccaataccca
    56941 cccgccgaca ttttccgtgg ctgtccataa acatcccgca tcctgacccg tatcggaagt
    57001 ataattgaca agactgatgg ttcccgaact gttcttaacc gcagaaagat taactccgtt
    57061 atctcttgcc aacagcaaat acgatgttcc gttaacaggt gaagtaacag gattaccgct
    57121 tgtatcacat aatacaatgg gatatcccgt ggcggcgaag gaacaagaaa ttcccgatgc
    57181 tatgtatctg tccggacctg tatacgaatc tcttattgta aggtttgatg tatcacctgt
    57241 aaatctgatt gctcgcgtat tggcagatac ggtaccgttt gccctgttat tgataaaatc
    57301 ccacaacccg ttattattcg ttatccgcca taccataggg tcggaactta aatccatacc
    57361 tccggtagaa gtatcatgta taatagtgaa tcctcccccc gttatatacg agaacaactg
    57421 catgtactgc gtggttgatt cagggtcata tgtagctgtg taaaagtcat aataatgttg
    57481 tttccatata tggttcaata tctcgtttct ttcggtctca gtaagagttt ctttaatata
    57541 ttcggcaaca tttctggctt caagattcaa tccgtcatct gctataccca aagaggtata
    57601 accctcggca tccctgcctg caatgatgaa atctccactc ttcaaactgt cattcgccgc
    57661 atcggttacc ctgcttacta catatgttgg cttaaacctg tatatgtatg tatgcgtagc
    57721 tgccaacatg tcggtagtaa gagagaaagt atttgtagta atatcataag taaggtaata
    57781 tttaattcct ccgctgatat aaaacatgta tttcgatata ccgtcatagt accattgggt
    57841 ttccggatga gtgctgatat tgacatttat actggttata cctatatccg aaccggaagc
    57901 ggttaaccag tctctgacga catttgtgga gtagtaatcc tgatagaaat acaatccttt
    57961 atcactgctt gtctgatgcc agtccacttc cacaggtacc attatgagca tatcggtact
    58021 gtgctgttca ataatattat tgttttcatc caggatattg ctgttattta tagcggcagc
    58081 ctgagaggtt acgtttgttc cgcatataac tttgttgttc aattcgtcta tagtttcacc
    58141 taaggcatag aaactggtag gagaatcggg attatccact ctggcggtaa tcatatagtt
    58201 catatccacg tccagattat cgccaatctc attaacctct tctataagga cattaatgtc
    58261 gtaacctgta gcttcatata aatacgggta tgtatattca gccggtttgg gactgcgcgg
    58321 gttggtatta ttggtgttta aagcgagagc aaatacaccg ggtgacggag ttttccaaac
    58381 ataatactca tatggactcc ttcttcccat tatgcagaag gagttaccta tgtagttacc
    58441 gtttgcgtcg gtacgataga aaggaccttg atcaagggaa acactgctgg caccaagccc
    58501 tattccggtg ggtgatccgc catcaaaagg aacaaaattg gcattaccgc tcatcctgtc
    58561 tacattaata ttagattgtt caagcactac caatgtacgg gaattcggat cattagatgc
    58621 gcctaaaaac atatgagttg catcatttac caattgataa ccgcaatcat atacggtagt
    58681 actggtc
//
LOCUS       DAOSOM010000010        55197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_631_length_55197_cov_16.737151, whole genome shotgun sequence.
ACCESSION   DAOSOM010000010 DAOSOM010000000
VERSION     DAOSOM010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 55197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 55197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55197
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_631_length_55197_cov_16.737151"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..623
                     /locus_tag="PLF14_03875"
     CDS             66..623
                     /locus_tag="PLF14_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HOT70469.1"
                     /translation="MINLQKGQRIEIGLSKVGVGLGWDPNESTGFDFDLDASAFMLGS
                     NKKLPQDEFFVFYNNQKSPDGAVESSGDDLTGGNSDGGDDETLTVDLTKVDPRIEEII
                     FTVTIHEAIARKQNFGQVHNSYIRIYNAVTNEDIARYDLDEDFSIETAVEFGRLYKRG
                     SEWKFEAMGIGYKGGLQYFVDKYAY"
     gene            698..1249
                     /locus_tag="PLF14_03880"
     CDS             698..1249
                     /locus_tag="PLF14_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HOT70470.1"
                     /translation="MINLEKGQRVNVDLPKFTIGLGWDTNSSSTGEDFDLDASVFILG
                     EDKKLISDDYFVFYNQLVSPDGAVEHTGDNLTGEGDGDDESIKIDLTSITPKASEIVI
                     VVTIHKAAERKQNFGQVHNSFIRIFNTDTQEEILKYELEEDFSVETAVEFGRIYKRNG
                     QWKFEAIGMGQKGGLQEYLNKYQ"
     gene            1256..1921
                     /locus_tag="PLF14_03885"
     CDS             1256..1921
                     /locus_tag="PLF14_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HOT70471.1"
                     /translation="MAIRLEKGQRINLEKNNGSKLTSFCVGCNWGAIEKKTFFGLSKE
                     TIDVDLDLSCVMFNEQGEICDHIYSPLYKPDLLSKFGLPPGKVDSLDRALHHSGDDLE
                     GDKGGDDGLDNEIITVDLNRLNPSIHQIYFFLNNCGQEDFSQIPYAAIRMYEGTPTRV
                     KEVFAQYDVAAESKYQGKTALIMGKLYKRNGEWKFAAIGDAYDDKFLGFTIHRIIKDY
                     VKK"
     gene            1927..2538
                     /locus_tag="PLF14_03890"
     CDS             1927..2538
                     /locus_tag="PLF14_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HOT70472.1"
                     /translation="MAIRLEKGQRINLEKSNGSKLENICVGVNWGAIKKKNWLGFTTT
                     EAVDLDASCAQFDANGNVIDKVWFRHLKSNDGAIKHSGDDLTGDTDGDDGLDNEVITV
                     NLNQLNPKTEKIAFVLNSFRGHDFKTIPFASIRIYEGTPTKVKEIFATYDIANDPTFA
                     GNVSMVMGVFYKRNGEWKFNAIGEPTKDRDFESTLQTVKQRYL"
     gene            2535..3173
                     /locus_tag="PLF14_03895"
     CDS             2535..3173
                     /locus_tag="PLF14_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016540376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOT70473.1"
                     /translation="MRRLPVYLLLDTSGSMYGEPIEAVKNGVQVLVSTLRQDPYALET
                     AYLSIITFNSSAQQVTPLTELSSFQQPNIDAGGCTALGEALSLLASKVDSEVTKTTAE
                     VKGDWKPLVFIMTDGEPTDDLNKGLAEFKKRKFGMVVACAAGQGANTDTLKKITECVV
                     QLDTADSATIKSFFKWVSASVSAGSMKVEETCQEVGGLSELPPPPPEVNIVV"
     gene            3189..3566
                     /locus_tag="PLF14_03900"
     CDS             3189..3566
                     /locus_tag="PLF14_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HOT70474.1"
                     /translation="MKTLQEYKAELLADGIIDANEVKELEQLLFTDGKIDSEEADFLF
                     ELNDAVSGKDNDCSWNKLFIRAIVSYLLEDKLSPGEIDDEEADWLYNKIKGDGQIDIL
                     ERELLLQLKSQAKNFPAKLEELL"
     gene            3684..4319
                     /locus_tag="PLF14_03905"
     CDS             3684..4319
                     /locus_tag="PLF14_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018678216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOT70475.1"
                     /translation="MRRLPVYLLIDTSGSMKGEPIESVKVGIESMIATLRQDPYALET
                     VNISIITYDRDVKVILPLTPLEDLQLPNITTPDSGPTHTGAALEQLCHQVEQEVTLST
                     TERKGDWMPLLFIMTDGHPSDIMVYNKVVPEVKKKKFASIIACAAGMKAKIEPLKLLT
                     DQVYSLDTMDSSSFKTFFKWVSDLIEVGNKSVGATDDLILPPPPEEVNVVI"
     gene            4327..4986
                     /locus_tag="PLF14_03910"
     CDS             4327..4986
                     /locus_tag="PLF14_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HOT70476.1"
                     /translation="MNVKLVGNFIQHIEAELMYGEEFYAQKGTLIYLEQGIEKDVVLN
                     GGGNGTLGTIGNLLGAKLTGESIFLVRYVNKCNKPRKVAFGGSFGLHPVKIQNESLIC
                     NRGVYVASNNLVQVSAKISIAGIVGGMGGLLQKISGTSTVFLDCKGQPITKELCAGET
                     IEVDEDHIIAMQGISEHQLSSAWSLKNVFGGEGLSMLRITGPGKVHLSPGSIIPQTTT
                     R"
     gene            5037..6089
                     /locus_tag="PLF14_03915"
     CDS             5037..6089
                     /locus_tag="PLF14_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerY-C metal binding domain-containing protein"
                     /protein_id="HOT70477.1"
                     /translation="MRRLPIYFLIDVSESMVGEPIEEVQNGIRTIIQELRFDPYALET
                     VYVSIIAFAGKAKTISELTELFKFYPPKFPIGGGTSLGVGLNHLMDSIDRDVQKTTLE
                     AKGDWKPIVFLFTDGTPTDNPDRAIQRWNTKYRKGCNLIAISIGDNVDTKMLGSITDN
                     VLRLKDTDANSFTAFFKWITASIKTSSVSVSETANDELKLAPIDGINLEKIDTNKPCR
                     IDENFAVVMGKCQTTKRPYLIKYAKRIRPLDVEGLEHFNVTDFKLVGAFPVEEESYNI
                     LADKSHTKRNINTIELLGAPTCPCCGNQFGFVVCECGNIFCVGDAEYNKCPWCGLEGT
                     LGEGKAEGMNVNRARG"
     gene            6094..7644
                     /locus_tag="PLF14_03920"
     CDS             6094..7644
                     /locus_tag="PLF14_03920"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HOT70478.1"
                     /translation="MTSIIDQLIETYFPQGVSSEKRSDIQKFFLEKIYNEYNEYYMDR
                     ELVIKNRIDALNLTLPNAKVREEYSQLIKIDDPDVCEYWLEGLEDTGLQAELEIILNP
                     AEEKTIPNDCASESAMPSNEDNVPIEVTDSTNLNEVAEVEKTDAQVLSDTRIVGTKER
                     GIVVKGIPTKAGDYEITLKYKYQNWSEGYSILERKFKVAVNPDPRSLWKDIPTDKNIK
                     FFKEDYSHEYITVVENEKGPQKDIVAASKRGRSHAHEGKARDDDFNIYHNDSNGWYII
                     AVADGAGSAKYSRKGSAVACETCVEFCKTALENPIELEKEIIALNSTTEGQSNRAIST
                     LIYNIVGGAAHKAHRAILETASANEDQPRDYSTTLLLAICKKFDFGWFVASFWVGDGA
                     MCIYDKERQYIKLLGTPDGGEYAGQTRFLTMKEIFTPESIMSRLKYSIEEDFSALLLM
                     TDGISDPFFETDANLNKVEKWNELWVNINSEVDLTDDNTESQYQLLKWLDFWSPGNHD
                     DRTIAILY"
     gene            7657..9132
                     /locus_tag="PLF14_03925"
     CDS             7657..9132
                     /locus_tag="PLF14_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HOT70479.1"
                     /translation="MSKTITLTATDGTKVEFIDDVIGSGAMKDVYFSPDKSYVVGFFR
                     EPQDANSKDRLTNITGTYRERIFNQVGGEYWKNLFCWPTKMVEWNGKIGIVCPTYHKH
                     FFFGSGNFKGKEKEGKWFASAKLRNRFLPAEEKGTWLTHYHMCIQIARAVKRLHAAGL
                     AHSDLSYKNVLVDPVTGKASIIDIDGLVVPGKYPPDVVGTPDFIAPEVLETRSLKIGD
                     PNKKLPSIQTDRHALAVLIYMYLLYRHPLRGGKVNDLDAAKDEELSMGSKALFIEHPS
                     DKSNRPKVDQLSKSELPQGDVTKLPYSICGPYMKELFNRAFIDGLHNPTLRPTADEWE
                     QALIKTTDLMQPCQNPNCEVKWFVFDNSTKPRCPFCGQEYHGQLPVLNLYYSPKKGVF
                     KPENYRLMVYNKQTLYKWHVNRFVTPNEKTSDEDKKPVGDFHFFNGKWILINRRLDSL
                     YDKDLDKKIEIGQYVELTEGKKILLSTEDGGRLIIVQLVNN"
     gene            9141..10301
                     /locus_tag="PLF14_03930"
     CDS             9141..10301
                     /locus_tag="PLF14_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 family protein"
                     /protein_id="HOT70480.1"
                     /translation="MIKEQCEQCKRNNTDCQENIEFNGLSCEQYAKRINLEKSEKNET
                     PDDSNVIQQNQETSATIEYPDPNYSIHGWLTFFLFSIGLGGLISALYPIFTYNIAEYD
                     GNHFLAISDVVLGVMLLILACYTIYSFCKRKPNAVFLAKMYVVVTFITNILALISGEF
                     EETGLGSLPRIVRSLVWGVIWFSYLTYSEQVKDIIPKSYRKVFSRDYYLMTAFIIIPI
                     LCLAIGVGNIFSQREEQEQNFISTTDLDYNEYTDGRIIFTKPDGFTCEKQELDDPKIT
                     VFDLESEYSYLRIVSDYDSDVSVQNFNTYWNGWKDDDLKDYSYKEITNEKREINGNPY
                     FIKSVKYDTETPIIWHFILLFSPKTGKVCVIHSIQIQDETLDIIDFIRTIRF"
     gene            10305..11342
                     /locus_tag="PLF14_03935"
     CDS             10305..11342
                     /locus_tag="PLF14_03935"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HOT70481.1"
                     /translation="MIYVKRNNQEFGPYDESTLLTYVNSGQILRSDSARNDATNEENT
                     VKYFLKKAGLKPKVPHKGHLFHQLAMIGGELILPKSTIISKQWLSDKRLLLLALIGLF
                     PTIFMAIPFPAMGIFYAIALYFSCIWGIFFYYFFRTKQVSLKYTLIVFFMTQLCIFFL
                     WDIVGWTKLNPFYNLVNLPFPFNFIGYTLGVGTTEEFAKAIPLYILIARAKEPLIPQT
                     LVFYGLMAGIAFGVYEGVGYQTSVNTQLDYTGAFFMNIARLTSLPFLHAIWCGIGGYF
                     IAFANLYPKYRISLYFLSLAIPALLHGLYDTLCGYSVLIFIAVGICFIAVLLLMTYLK
                     QGVNYQSKLRN"
     gene            11349..12176
                     /locus_tag="PLF14_03940"
     CDS             11349..12176
                     /locus_tag="PLF14_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70482.1"
                     /translation="MGKCLYCGQDAGFFRNKHNECEAKHLKGLSFIKQIIIDCFTHKE
                     DFYLKDSEIRKIVQDSHINKTSLDNMFCSAFDEAIDGYLNDGIVDSAEEKTVARFIQY
                     SGLSQSFLNANKSLEKVLQSKVLRDILSGNIPTPQITIAGDFPFLLNKSEHLVWLFRN
                     ITLYQQKIQREYVGRSRGVSVRVMKGVYYRTGGFKGHPVETTVMQKIGSGSVCLTDKH
                     IYFASPEKSLKIPYSKILSIESYSNGIGLQKDGANDKPIFLEGINSWFAYNVVANYK"
     gene            12182..12682
                     /locus_tag="PLF14_03945"
     CDS             12182..12682
                     /locus_tag="PLF14_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70483.1"
                     /translation="MYNLEQIKQTIIANGSITSSDIPDLRKIVYKDGRVSREEADFLF
                     DLRKEILNFGNLKEWDNFFIQAICDHLLEESHDTITDDDVSWLIDKIGADKYIDHIEQ
                     ELLCKLSSKVKYFPSNLKAIQEHTNIAYDLGCKILSLLCRNSKTGRSLQFGINKVGCR
                     KNKIVV"
     gene            12788..15643
                     /locus_tag="PLF14_03950"
     CDS             12788..15643
                     /locus_tag="PLF14_03950"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOT70484.1"
                     /translation="MEQKKNYIGLTDAQVLESRKKYGANILTPPEKDPWWKEFLEKFS
                     DPLIIILLVAGVLSIGISFYEFFGLGQNWKVFFEPIGIFVAIGLATTLAFIFEQRANK
                     AFQILNQVNDDELVEVMRNGTTTTIPRKDVVVGDIVIINTGDEIPADGELLDSVTLSV
                     DESTLTGEPLCAKTIDPAHFDKEATYPSNHVMRGTKVMEGHGVMRVLKVGDATEMGKV
                     FEEAQIDDSVKTPLNEQLDGLADWITNVSYGFAGLIIVGQLIHFLGWSNWQAWTLIVP
                     VALFFWLVIKKFEDWSKAKCIFTIIGFFVLFFAMVIGAFSMIHAGADSTTWSLLLAHT
                     LKTLMVAVTLIVVAVPEGLPMAVTLSLAYSMSRMLKTNNLVRKMHACETMGATTIICT
                     DKTGTLTQNQMQVYKTNFFGEPSDEILYEGIAVNSTAQLDLTGDKPQVFGNPTEGALL
                     LWLKEHNTDYKALRMNATRIEELPFTTERKYMATVVKSATGKNILYIKGAPEIIFAMC
                     KNTCGVSKQEIDAQLLAYQNQAMRTLGFAYQELGDKDATIENGKIVADKLTFLGIVAI
                     SDPVRLDVPDAVNEVIAAGIKVKIVTGDTPGTAKEIGRQIGLWNDATDTDRNIITGVE
                     FSELSDAQLRDRVGDLKIIARARPMDKKRLVEALQTNNEVVAVTGDGTNDAPALKTAH
                     VGLSMGDGTSVAKEASDITIIDNSFSSIGRAVMWGRSLYKNIQRFLLFQLTVNVAACF
                     LVLFGSFMGTESPLTVTQMLWVNLIMDTFGAMALASLPPSASVMNDKPRRREASILTR
                     PMMTELLGVGLFFFALTLGFYWLFNHAEVTSITQMFSAVVGGENPMTAYEATLLFSIF
                     VWTHFWYMFDARVFETGESIFKVKMSRGFWTIVGIIIIGQLFITEIAYEFFNVEPMLH
                     TLDWHFNPTGAIDLLIIVSASSLVLWVREICRLFTKK"
     gene            15706..17763
                     /locus_tag="PLF14_03955"
     CDS             15706..17763
                     /locus_tag="PLF14_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT70485.1"
                     /translation="MKRIIITLFVVIGMTCSISAQNIKRPDSYNYNRGVEAIQNNNAE
                     EALEYLNKELEDNPNNGYALAWIATVRNYQEEYGRALTAVDLAIKHIPKKDKQYRAFA
                     YIVRAEVYVGLGENEKALVDFTSAIKETPDAADVYEKRANLYYYMDKYDLADKDYQKI
                     ISIDPGSVMGYMGIGRNANAEKRYNDAIEQFNYVTRLASDYSSGYSFRAESYFGLQQY
                     DKAIDDVIKALDIDGDNKAFHLMQQVADSAMIPLVAKLKVQSAKNPNNEYWLYCLGIV
                     HERTDAYKKAIEYYTSCYNKDASDVVAYRISQCHSEMGNFSLALDHIDNAIALDSTDF
                     DYVMEKADLLYESGNTKDAIVESGKYINHYPDYFGGYYRRGWFKDNSNDTDGAIEDYT
                     MAIVLQPDYAYAYLGRGDMYILKGDKKSAEADYRKVIELDTIPSNSSCAQYAYWGLGQ
                     KEKAIEFMDKVIANDSDNAGNYYDAACLHSRMGELEKAIEFMRTALEKGYRRFAHLEM
                     DDDLNPIRELPEFKEMIAQYKKRFESEIEGKTQDTSLYEEKTVEIPFTKEDGVCKVKC
                     AINNLSLYFVFDTGASDVSLSSVEATFMMKNGYLKPTDVIGKQNYMMANGEVSAGTVV
                     NLRNVNFGGLDLDNVRASVVHNQKAPLLLGQSILNRLGNIKIDNANRVLKITYKKKVE
                     ENK"
     gene            17760..17966
                     /locus_tag="PLF14_03960"
     CDS             17760..17966
                     /locus_tag="PLF14_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT70486.1"
                     /translation="MKREINRLKVVLAEKKRTNRWLAAQLGKNEATISKWCTNKSQPS
                     LDDLVAIARCLEVDTKDLLHSITE"
     gene            18091..19389
                     /locus_tag="PLF14_03965"
     CDS             18091..19389
                     /locus_tag="PLF14_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1810 family protein"
                     /protein_id="HOT70487.1"
                     /translation="MNSAKYEEYISKYNLRRFVDAHRQIYGSALTEIRSGKKQSHWMW
                     YIFPQLRGLGHSRNAEYYGIIDRDEAIMFLLHPLLGRNLYEITMAMLTIDGKSAEEIL
                     GGIDALKFRSSMTLFDNICPNDIFSDALQKYYDGKSDERTLEMLQTSKVKDEQLISGG
                     IIGAIIGDIVGSFYEFCNHKSTNIALFTRGTQFTDDTVMTIAVADWLLSGVPLREAML
                     DWGKEYPNREYGGMFYEWLFYSETKEPYNSFGNGAGMRVSPCGYYATSLDKALELAKQ
                     SAEVTHNHPEGIKGAQAIASAIFLARQHKSKDEIRDYIEQTFGYNMHRSCDEIRPTYQ
                     FDVTCQGSCPEAIIAFLDSHDYESAIRLAVSLGGDSDTIACMTGGIAAAYYGIPSWLV
                     KYVTVEFLPQYIRDIIAKFDKVCAERIAIQSKFDNKETNR"
     gene            19561..20940
                     /locus_tag="PLF14_03970"
     CDS             19561..20940
                     /locus_tag="PLF14_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70488.1"
                     /translation="MLLYTALYAIYWETVPKDKRLRWLFLPYFMYSGIAILFCGLIKT
                     WNVSVWYSFLLPIYGATCVILAKICGKQIRRIRKKYKFGKIITYTAVLLFFVALKSIS
                     VSWECSKRNSFEKEDILARKDYLLGKLMVNPQVVLNEMPAAIGTQFQGEWALYSCSML
                     SAALVNISQLYPETKTGNIQLIDSLINIVMSPELRYYDTMRWNEDPLESLDGDESHIS
                     YLSHLAWMICGYKQIGGNSKYDKLLFSLCSTMNRRLLNRSSLNLPTYPGESIYIPDML
                     VAIVALNQYADMNNGKYRSTVKKWIKRAQKEWIDDKTGLLVSFLQENGNQYEEVPVKG
                     SYSALNCYYLTFIDETFAKQQYETLKSLFWKDGVISGLKEYYDRTCYLGLDIDAGPIL
                     FELSPSGTAFMTGAATYFNDLKTRNDILTTAEIAGHTVRLNCKRHYLLANVALVGEAI
                     MLAMRTHFK"
     gene            complement(20933..21559)
                     /locus_tag="PLF14_03975"
     CDS             complement(20933..21559)
                     /locus_tag="PLF14_03975"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOT70489.1"
                     /translation="MKAQFNTISCPGNRYKVVVENPSQTTNETEATPESITSENEKSV
                     PDKVALSSTDIKKKEQVACYLSVCYPLSSVKINSPYGYRKDPFTGKRKFHNGIDLHAR
                     SSKVFAMMQGRVLKVGQDKVSGKYVTLQHGSFTVSYCHLSQIFVSQGQDVLSGDVVGI
                     TGNTGRSTGEHLHITIRQKGEYVNPRIFLDYINSVKESCVMEFVKNII"
     gene            21834..22592
                     /locus_tag="PLF14_03980"
     CDS             21834..22592
                     /locus_tag="PLF14_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4099 domain-containing protein"
                     /protein_id="HOT70490.1"
                     /translation="MKQVRFEFEELPYDTLSQFGLTQEMIEDLPMQTLEAISHGKHSP
                     VLPIKVNDENGNTVTDRSRFAFVRKENGEADVIFFPVLKKSPLEKYNEEQKKQLQSGK
                     TILADSVTADGRKTKVFVQIDTETNQVMSVPTQVIARNLQVLAEELKLSNAEIKVMQQ
                     GEPLTFVMEDEPVTVGIDLNEKNGIRFCQGDCQKWSEHTKREWDKYTFGCYGCWVMDD
                     DGCLDYVPEEQYTEELWNEQKKSAQRNMGAGLHK"
     gene            22611..22835
                     /locus_tag="PLF14_03985"
     CDS             22611..22835
                     /locus_tag="PLF14_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70491.1"
                     /translation="MAQNAFYPEDVLIEKMESGEYGWLDYVNHFSAEWQDELAEYCTE
                     RALEINDASAEQFVHYKSEQLEAAMESGEA"
     gene            22841..24457
                     /locus_tag="PLF14_03990"
     CDS             22841..24457
                     /locus_tag="PLF14_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70492.1"
                     /translation="MAIRTHTNPRIADLQPEMRDLLMRNGMQAHIVPAGEGYQLVVQG
                     HDSPLLRYPISKQQFSALTDWGTNHANKKAYNTFTEIVKDDFDLPRNFVHARNANGRV
                     AMGLHGYRIGIGEYGRVGRYGMPPPFLGWTPRQQEGYHLRRVGGRLFYAGTPMVAERP
                     GGRMKPGELQSGGYGFYYKGHPQQPAQPVRQDVLQELQAVITPMVGKPRSEEPAKPYK
                     ELITSPVYFSNEKWQECLSSHGILVDKEEKTLTIQSEQIHADMVYDITDAELSALTSN
                     SIEEHPIERRLELLNTVIQEDFADKITMDSLNHTERIGITLHPEVEQDLAARQQQEQE
                     ILLPLEADNGLAPYSNEQAILQEDESIIREPQEGALIDGRDLPYLHESKGWYREGEHG
                     REVEVEAIAVQSAEAEGKYRMTAIINGEAITQEISQKQYDKFLAVDDYHRMKLFSKVF
                     NEVDMKTRPEMRSNLGAKIFAALAAGTVVASDLVHGIHHHPCPELYGEHFCHAPRPYF
                     KPGVDSPRDVAARNFEAQMNQEIAEIRRGF"
     gene            24474..25646
                     /locus_tag="PLF14_03995"
     CDS             24474..25646
                     /locus_tag="PLF14_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOT70493.1"
                     /translation="MANGELTYDDFLQRLDIQDVLVDAGYHLNKRDGLRYPSYIRTDS
                     NGTRIRGDKFIVTGGGKCCFQPPQQKLYNIISFIKSFPEKFAEHRNGVSPDRLVNLVC
                     NRLLNHPIENRPICIIQPKQHSRPFSLQDYDIHHFDVNDRETHKRFYPYFKNRGIDIF
                     TQRAFAGHFFLATKHRTDGLSYANLAFPLVLPKEPDKVVGLEERGRPKMDGSGSYKGK
                     AEGSNSSEGLWIANFSGEPLHQAGGVAWFESGYDAMAFHQLHRDTFRDNPELSKKSVF
                     VSTGGTPTDMQIRGMLSATPNVNHYLCFDNDSAGREFVNKFRLIAKSMHINPEQIREI
                     PLMPCYKDWNDALLGKTSEEYLDSIKDAVIPLSAPLGTKGSTAGEEEVNEQSTIHR"
     gene            25652..26140
                     /locus_tag="PLF14_04000"
     CDS             25652..26140
                     /locus_tag="PLF14_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOT70494.1"
                     /translation="MQNRQLYRDISLRPKTTQFFIDELPLLFVAITGLVYGGMDDAPL
                     GSIATLFAVLLSLYLAYQLIYLKRIRYHIGSEQLTAAHGVFQRSTGYIELYRVVDFHE
                     HQTLLQQIFGLKTVTVLSMDRTTPKLALIGLPKQTAIVELIRERVEFNKKRKGIYEIT
                     NH"
     gene            26121..26810
                     /locus_tag="PLF14_04005"
     CDS             26121..26810
                     /locus_tag="PLF14_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70495.1"
                     /translation="MRSPIIKILVLPLVLLLTHTAKAQIVTANPLEYVALAEGNELIL
                     AKVRDQIDGQKKTALLQNTIAAEFEQMRQWEKKYNSYLKTASGFASSIKACTHLYNDG
                     VRIFITLGKLKNAVSDNPQGIVASISMNNLYIETATELVTVFSLLRDAVAKGGKENML
                     TGAERSQTLWALNDQLSAFQKKLNLLYLSIRTYTMTDVWNNATAGMLDRNNGEIAQIA
                     MRRWRRAATIR"
     gene            26827..27507
                     /locus_tag="PLF14_04010"
     CDS             26827..27507
                     /locus_tag="PLF14_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008775167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70496.1"
                     /translation="MRRTIFITILLMCMGIKVHAQLDPTLTGMVLIYTEKAKKTLKNQ
                     EKVMLLQTTGHIWTKEEVEAVTDLQREFNDYLDSFRSVVSYAAQIYGFYHEITHLTEN
                     MGEFTGQLKNSPTNALAVALSTNRNKIYRELIYNSIEIINDIRTVCLSDNKMTEKERM
                     EIVFGIRPKLQLMNKKLQRLSIAVKYTSMGDIWAEISEEARPKANKVEIARSAIRRWK
                     RSGKAGAY"
     gene            27531..28202
                     /locus_tag="PLF14_04015"
     CDS             27531..28202
                     /locus_tag="PLF14_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008775168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70497.1"
                     /translation="MGFWSSLLKTGGKAISYGGKATVATGKSVGNAVLHPSQTLRGAG
                     SALKTATVGGAVGYVGWEKLTTDKSVVRIVSDAVIGEEATNAISGTAKSAGDKVSQLT
                     DKAEQTMANVNQATSNLSSTLNGASNFLGNVSNGNGGNMLGNFFSNLANGKVSGMSIA
                     GLIAGAFLVFGRFGWLGKIAGAFLMMMTIGNHANLLQETRVANAPRQTPSQQEEQTHS
                     GGMRR"
     gene            28231..29061
                     /locus_tag="PLF14_04020"
     CDS             28231..29061
                     /locus_tag="PLF14_04020"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOT70498.1"
                     /translation="MKYTEEMILHSPSGYCMPFEEDKNKEVTLSKGYGEQKDAVTGET
                     FFNHGIDFNASHRPLAAVASGVVSSIGTDKERGVYIVIRYGKYEVTYAHLANIFIRFG
                     QKVKAGQTVAISGSDLHMEVAFDGEELNPIEFLTMLYGNIQALRKAGHEATHEFIQFD
                     GEMKTRYDRDKEEIEELMIRFLPVYMEDLFRGAYTVPEYTEQSLRNVFAVGATKNYFF
                     ETMPSMANPLGIGSRALPLASKVQNLLIADFLNYLALRHNVYLSTMDEELKKNFNPRP
                     "
     gene            29085..29366
                     /locus_tag="PLF14_04025"
     CDS             29085..29366
                     /locus_tag="PLF14_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70499.1"
                     /translation="MEELEIDVQSFDIPRVVSVYPDRAGVRWWTKAWFNNREEGELSV
                     EIDRNNAIRFIQDRIEKDEWLEEFFPKQMEIYHNAIEQTKEQLLNQLNY"
     gene            29370..31211
                     /locus_tag="PLF14_04030"
     CDS             29370..31211
                     /locus_tag="PLF14_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70500.1"
                     /translation="MGEIRHTERFAEIERIFGDYFNCHLAPVMRDTQSYLNRKQVEEM
                     AEYSTSAAGILSSMAAANNPMIDPYQTLKITGEWNSKTTEDYLAMVKDKISGNKDMQT
                     DLAMMAGEWRSAVIGEIGRDRYDTLSGQLGCDLAYAYTDYRMEQLMIDRLVKERMPKS
                     SADYIIRKAAQNTLLGLSATLGQSPLAAEIEQRGEAAYKPSKLEKGTSRVIGAGIDAL
                     SLGGVGSWTSFAKFVGVDLAFSALTPSQDNKGNGQSVEDCISKGVFGSEANVFADFRK
                     QARSIQKDENPYIIATNERLAKKIPIETFDFTKWMSEHTITSKPMWSLADEKKNEERY
                     KNVPLVIMPEYREAYLNSLEQKQNKGSEESASTEIAKQEVQNTTEQAVTQQIEESEMS
                     GQSHSEAITQEEQGQQTVQSDTRTNENGWNGLFSSLGLNGMGDIGRNLSYVLSMLPDI
                     LVGMFTGRTKSLDIGDNIVPMASVLAGLFVKNPVLKMLMIGLGGANLLNKAGHEAIDW
                     KRNEGNSRPVSENRPVQYRSYPDEPLNPRISNPILKGNNLIATIDRVPCTIQLPEKVT
                     AAYHAGALPLNTLANAILAKSDQTRAMMERNFENGERETIIRTRGIQ"
     gene            31225..33225
                     /locus_tag="PLF14_04035"
     CDS             31225..33225
                     /locus_tag="PLF14_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC family protein"
                     /protein_id="HOT70501.1"
                     /translation="MKEKSQFEKNAADKQVELLSEALNGAVSAGGHWLNATGKGYPKF
                     YPKGVAVSPFNALFMALHSDKTGSKTNLFTLYSEAKARGESVREHEKGVPFLFYNWNK
                     YVNRNNPNDIISRNDYQQLPPEERNRYKGIHNREIRTLFTINQTLLPLVDKNVYETLV
                     QKHGNSVEQGFGEKELRELRPRFNAFVQAISKNMVPVRTDGSGVAHYDSQKDAVYIPR
                     QKDFEHYTDYAQETLRQIIAATGHQQRLAREGMVMKNGVPPTEDAVKQEHLISEIASG
                     IKMLEMGLPARLSDENIKMVDYWNRELKENPNLIDAIESDVNNALEVIRKAERGEKIE
                     YATFRNHRQTERMQEQMPKHFFVADEIKKHPDKDNKTIVLVIDRQGKSADVILPAGAS
                     LEVNNEIKGMNKQRIQSALQKSGIEHVRFYNPDGALGYRPDDRYFAEKQIEVARLKSW
                     TLETLSTIDAHPAVKQAYDLGFEQIQMVQDDKNRWALYIKPEGKSGYSIYPDKDDLNR
                     FFTTLKQSLDNIDKVRGELAKKYYALAESKPELKVDLFHTSDERIDLNRIQRVAVFKT
                     KNGAILCAPTIDNQKPQPRSVTPQQWQRMWLAEDRNSFKQHLAATLFADLLNKSQSQE
                     QTSSEKQEEEVEAKVGRKEEIKPEQPKEEVEEQEEASRGMHR"
     gene            33238..34782
                     /locus_tag="PLF14_04040"
     CDS             33238..34782
                     /locus_tag="PLF14_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HOT70502.1"
                     /translation="MSKNQQYAEQYADFAMEQMRKYGIPASVTLAQGILESSNGQSRL
                     ALNENNHFGIKATPRWIAQGGKYGLYTDDKPNEKFCSYDSVGDSYEHHSRFLVENKRY
                     DRCFALAPDDYKGWTEGLAKAGYASGSNYAGSLQKIIEVNGLQKYDRMVMAETKTQRL
                     EIGQEMNVDANKEAYSFPVERAEFMFVTSPFGMRTDPMDAGKQQMHKGIDIRCKGDAV
                     LATENNGKVVAVNQNANTAGGKSITIEYPRSDGSKVQNTYMHLSSVDVKVGDTVQARQ
                     KVGVSGNTGTRTTGEHLHFGVTLVSADGQKRDMDPAVYLAEIAEKGNIKLQALHNGND
                     LLAKYKTEAVATPQESKAQSPEDWMKKLLSSEDSGVGLSGTNDPIMDMVVKAFSSLMM
                     LAVVIDSKDEEEQISAVSKMADERKVDLTPLLPHMKTCALVVGENNKAVLQADNGSIQ
                     FSRELSANELSRLSATLNSPGLSEESKRMRVAGMVNALVLSQQASQNFEQGMSEQQSR
                     EEQIKR"
     gene            34782..34955
                     /locus_tag="PLF14_04045"
     CDS             34782..34955
                     /locus_tag="PLF14_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009036722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70503.1"
                     /translation="MKRVMMEIGLLFVGLSMAALHTLVCYKLFGLVATLVIIGVQVVI
                     AIGIVWIKIRAPD"
     gene            35036..35644
                     /locus_tag="PLF14_04050"
     CDS             35036..35644
                     /locus_tag="PLF14_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008781738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70504.1"
                     /translation="MIKCNVTMCGTISRVATVRTNSENKVFTAFAVNCVVPAKSGINK
                     TVEISVAKDGEDNELKNCALGQRVNVSGTLTFKKRGDNLYFNLSASEVTLSASNEDSI
                     KGEMHFRGKTGKIIEEKTDKKGKEFLQFSGFSAEKVNDGFEYLWVRFFGFDCKREEWL
                     QPQTGIEVKGDMELSVYNDKLNLSCKIAEMSAYVKPPYTPNN"
     gene            35650..37362
                     /locus_tag="PLF14_04055"
     CDS             35650..37362
                     /locus_tag="PLF14_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008782469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HOT70505.1"
                     /translation="MAGYKKYNNDGPSAEDKALDLFAEMMIERIETISKDWSKPWITE
                     GSLGWPKNLSGREYNGMNALMLLLHCEKNGYKIPRFCTFDCVQRLNKPTEKQAKEGVE
                     LPRVAVNRGEKSFPVMLTTFTCIHKETKEKIKYDDYKRLSDEEKKMYNVYPKMQVFRV
                     FNVSQTNLQEARPELWNKLANGDAVKLDESEKMSFEPMDVMIRDNRWICPIKPMHQDK
                     AYFSITKNEIVVPEKAQFKDGESYYGTLWHEMTHSTGIEGQLDRIKPTAFGSDEYARE
                     ELVAELGSALVAQRYGMSKALKEESCAYLKSWLEQLKESPQFIKTTLLDVKKATSLVT
                     QNVDKIAEELEKGKKEEQDNKQGMKVEQPASGEKIFYSSVAYLQSTDDTSRLDEFRDK
                     GDYEGLLQAAKEYYDGNGINEQYTFASPLQNKGDDLLIEDKDFAVVYNNSVGGTYEVM
                     LKYSEQEIRDHITRYGTRLASDDIKEVAKDMVAEQFQAITKQRIPVFEMPSGDILYAG
                     YNRETDTLDVGTVVNAGLVPNHQFPYDHDNSLESNLQSVHSELSHMEQYQEEEVEYSG
                     GMHR"
     gene            complement(37442..38068)
                     /locus_tag="PLF14_04060"
     CDS             complement(37442..38068)
                     /locus_tag="PLF14_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70506.1"
                     /translation="MEMTTIHPAESYLRNETNPPSLYVRIAGKRRRLFINRDRGIIGI
                     IALGKRTKGYVFTDWASIEQIYYPSQEQDENTDRKLILKYQKLARLATHTNSWLREIA
                     NADLDKNLYENHITTGTRIDGKCIGLATIEKYCGTTAMRLFREALKNKEKFSTCRFDF
                     CGYDGTLWCEPRDNGDMAAGFSKEYRNCGNGYYYLLINDDYVIGYDID"
     gene            complement(38077..38304)
                     /locus_tag="PLF14_04065"
     CDS             complement(38077..38304)
                     /locus_tag="PLF14_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70507.1"
                     /translation="MLDEIRQNGKTVLSGEDGFSIPMFFNNLCGKNFSGEKYRDYIKY
                     IAFGEMGFKPGGIMLYRNGTLVRTGKINIEK"
     gene            complement(38298..38549)
                     /locus_tag="PLF14_04070"
     CDS             complement(38298..38549)
                     /locus_tag="PLF14_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70508.1"
                     /translation="MTLSNKSYYRRLCRNILADRFNWRKYCTPSLYFGREICVTPLHC
                     SYGQIGYTINFPYTNAPEVEYDWEMNKLTIDDENWKLVC"
     gene            complement(38561..38923)
                     /locus_tag="PLF14_04075"
     CDS             complement(38561..38923)
                     /locus_tag="PLF14_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70509.1"
                     /translation="MITEEQIQRITDIAQEKGWSVNVEDKNETRVLFEFQRYTKYGQD
                     FLFNADMQGEDIDSLIANVKEYYEGFDPDYEAYLWIGDDGHGKNGAPYHIKDIVADME
                     DAEEQIYELLQAFEMEFL"
     gene            complement(38975..39307)
                     /locus_tag="PLF14_04080"
     CDS             complement(38975..39307)
                     /locus_tag="PLF14_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70510.1"
                     /translation="MKALQIAIPRWNITELNGYEPITTFWQDFSIADKFGDKAIADTY
                     RRAKSEWKDNYKYWTELCLVLNHKIWQWHERDNQRAILYDKLWREADEMTSEWSDEKQ
                     EYYFQVTD"
     gene            complement(39313..39624)
                     /locus_tag="PLF14_04085"
     CDS             complement(39313..39624)
                     /locus_tag="PLF14_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide:quinone reductase"
                     /protein_id="HOT70511.1"
                     /translation="MEYNNQLSENDKRFADEFSNYVNGKMASPRKVGKALADDHRYLV
                     NEKAKLMFYFMEQLAENWHKGRYDQRNEWACRLAAEAIDHLAENDLYHLPEEYYENHK
                     Q"
     gene            complement(40027..40473)
                     /locus_tag="PLF14_04090"
     CDS             complement(40027..40473)
                     /locus_tag="PLF14_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70512.1"
                     /translation="MEKKGYGIGKRTKREIGTPLMVINIKQEIIDILQIANRRFMYNP
                     KIGILILGDEMYGKNICSSHSQEFYVSKADGHFDDYLRGWIGVSKSYPRGVIHFAPAV
                     SKEQFDRGFDSLQMFSKLDGVNGDTIIRGFCGLPEQRMSDLLPSSF"
     gene            complement(40498..41079)
                     /locus_tag="PLF14_04095"
     CDS             complement(40498..41079)
                     /locus_tag="PLF14_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70513.1"
                     /translation="MPNWCSTAYAIEGNATELKKLYELMKDLEKRQKPSVQNGFGTTW
                     LGCLVEALGESWEKIYCRGTWDNLKFNGEVLTFNTETAWSPCNEMFDMVCEKYPTLRY
                     FYQSEEPGMCEYWTNDDEGKYFPDRYYVDVCTSEEEYYSEYFQDLESVYDWLEDICEV
                     QVKSRQDVNAIVKQWKEENEDAYCSIHEYQISA"
     gene            complement(41629..42894)
                     /locus_tag="PLF14_04100"
     CDS             complement(41629..42894)
                     /locus_tag="PLF14_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008781727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfJ domain-containing protein"
                     /protein_id="HOT70514.1"
                     /translation="MRAKTRLQHKVVTANERLLPLTEKQELWAFRHCLNHYAFRTKSG
                     MTTCMDCGHQWSETREKTCRCPRCDTKLEIRNTLQRKYKDKSYYSILTTQDGLQVQRV
                     FLLTAHYRKGKKADFYSMEVVRYWIDDNGKTEVTALRRTLGYYIDSFVFDNHIELRND
                     NNVYRRIADCKVYPYYSATTKLRRNGLRGSLSEIEPTKLIEALLQDSRAETMFKAGRK
                     VDLNYFLQHPMYFDLYWNTYKIVMRNNYHISDISLWVDYIRLLERCGKDIRNAHYVCP
                     LDLKAEHDRYQERLRVIQEREKREAEQKKAQENEEKFRELKGKFFGLSFTDGLIVVSV
                     LESVDDYYKEGNALHHCVGQCEYYLKPKSLVFSARINDKRIETVELSLENFKVLQSRG
                     LCNQNTEYHDRIIQLVQKNARQIRKRMTA"
     gene            complement(42891..43331)
                     /locus_tag="PLF14_04105"
     CDS             complement(42891..43331)
                     /locus_tag="PLF14_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008781726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfK-like family protein"
                     /protein_id="HOT70515.1"
                     /translation="MKGTEQFKQTIKAYLDERAKTDELFAVSYAKENKNLDDCITFVL
                     NQAMAICKEGGCGMTDNEVYSLCTHYYDEDNIEVGKAVNCGVIVNHRVELTPEEQAEA
                     REKALKAYQDEELRKIQQRNSRPKPTQKATKQEVTQPSLFDLGL"
     gene            complement(43340..43531)
                     /locus_tag="PLF14_04110"
     CDS             complement(43340..43531)
                     /locus_tag="PLF14_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009036713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70516.1"
                     /translation="MRTRRMTKEQGERCNISRFPNFHNTGSVKGMKRLYYGKQALLVR
                     CGAYIYNVSSEPSIYYQAK"
     gene            complement(43536..44021)
                     /locus_tag="PLF14_04115"
     CDS             complement(43536..44021)
                     /locus_tag="PLF14_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008669119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70517.1"
                     /translation="MHSKIFQITEERPLKDNILNDCTLEQGDGHYYDYCSEIDEEERK
                     SHIDNLINNILPKGMFELVSDDTICYNGGADKWKEEFVATLQEKAKAVTTENCTLWIG
                     EVYQLEKYLKNPLDTAYQFYMNEQCINGAAEQSYEFLRVVSQLEPGTLLYIGGVIDYH
                     F"
     gene            44540..45268
                     /locus_tag="PLF14_04120"
     CDS             44540..45268
                     /locus_tag="PLF14_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HOT70518.1"
                     /translation="MTAVNQIIDIAKKQDGIFTRKDLLNVVRSGMKNISEGSLVVLLN
                     RMIAENKIIRVSYGKYKLNEDLKHEFLYEPNEFMLSLNKHIKEKFPFIDYCIWQPSVF
                     ASMMLHVPAVRTTLVDVEREAMESVFMSLQNVESEIPIFLNPSQEDVDRYITNRDLII
                     VRPLVKEAPLDVINGCPVPTLEKMLVDAISDKELQHLQGNELYTIYSNAFSDYAIKKT
                     RLLRYAARRNRKQKVEQIINTINI"
     gene            45265..46329
                     /locus_tag="PLF14_04125"
     CDS             45265..46329
                     /locus_tag="PLF14_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOT70519.1"
                     /translation="MIHPDSRTLSWIEQVAKDNKIKDITLVEKTIRAFSLLEALARSG
                     CPFLFKGGSSLMLHLDTGKRLSIDIDIICPPGTRIEDYLEKYSEEYGFGEVKLVERIS
                     RTDIPKQHAKFFYQVAYPAGGRQDKILLDVLFEEIHYANVVELPIQSRLLKTEGDPII
                     VKLPSKEDLLGDKLTAFAPHTTGIPFFKGEKNCSMEICKQLFDIASLFDIVNDLSITT
                     ETFNKFAMVELQYRGESPDAIQKVLDDIYDTAKCIVMRGQDNQEEFDMIQDGIKKVRG
                     FIHSEVYTLESAITNASKAAYLAKLIGKGIHEVKHYNPSEANLLINTTIQPPLSTKLN
                     KFKKTNAEAFFYWSEIQKLG"
     gene            complement(46414..47910)
                     /locus_tag="PLF14_04130"
     CDS             complement(46414..47910)
                     /locus_tag="PLF14_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOT70520.1"
                     /translation="MRNKIVFVMAMAGMCCTLQAKANVQPVPQDSVHYESYMEKELMF
                     QTAEPTYHKGVLVTSPWNGNWFVSLQGGASAFIGRPIGCADLFDRVQPSLSASVGKWF
                     TPQIGARIGYGGWRFKDCNLTANDYHHFHADLMYNVLGGLYAKKENPRWGIVPYVGLG
                     MMYNPQNGQKPFAISYGIQGQYRICKRLAALLEIGNASTFQNFDGYGEANRFGDNMLS
                     VSAGLSFTIGKVGWKRTVDATPYIRQNEWLVEYASELSESNRRYIGQHDRDMRTLNEL
                     KKILDIEGLLEKYSRLFEDHENLSSGYPKNDYSGLNSLRARLKNRRWDGKSPLSNDSL
                     AVCSGIANVNVDDSGNSNEVQSDNLAKAQELTNMGGDSIATYNHSDYLSFINSGNECI
                     GSPVYFFFELGTAKLTDKSQSVNLDELARVAKKYGLSVTVVGAADAATGAADVNDRLS
                     ISRADYITTELVKRGLAVDVITKVGKGGISDYNPTEANRHTKVLLYMK"
     gene            complement(47966..49417)
                     /gene="mobV"
                     /locus_tag="PLF14_04135"
     CDS             complement(47966..49417)
                     /gene="mobV"
                     /locus_tag="PLF14_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="HOT70521.1"
                     /translation="MANAKQVMDFRPSKGITTAQSNEHQRRWTEKGWERAVSVGNYDP
                     TREHLNFEIRAGKVCPIDKTRSIPERMSDNLLARGIKDPNAGLAEPRFRTVVNFIFGG
                     SRERMHEIAFGSQIVNFDKGADNSHVERCKDIEEWAKDVYSFVCGKYGEENIAAFIVH
                     MDELNPHAHCTLLPIRDGKFAYKQIFAGKDKYEFSARMKQLHSDFSEVNKRWGMSRGS
                     SISETGAKHRTTEEYRRHLSEECTNIEEQVVQHKKALSDLKVEISLAERRVKGLTSMV
                     DNLRKAKAEKEYQLSALERTLQSHQGDTAAIIAERDRLEKELASIQTKLEDKQDKLRT
                     ADQQLEALKRDMDAIGERTEELKGEAYKYIREIHSNVDVLLKDVMLETLVREHSARLG
                     EMGLSEQSRFDGSLLQSLTEQGSEVMHCATLLFLGMVNDATTFAETHGGGGSSSDLKW
                     GRDDDEDNRAWARRCMMMASKMMCPASGKKQKR"
     gene            complement(49649..50500)
                     /locus_tag="PLF14_04140"
     CDS             complement(49649..50500)
                     /locus_tag="PLF14_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOT70522.1"
                     /translation="MTKDKNRCANQILADMELFDFLKEKVGERKTKTGAYHDLLEKCV
                     AGFVVPFLKNHEYVLQADQCHVTISDLASEWHWHRATVRTFLDKLEDMGHIYRTRFAK
                     SVVITMVFNKTGIGNSTTTYNGQAEIRPADEFDAALSKWITGNLTDSQMGDICEQYYV
                     SRLKEIADNSPVGGSGSQTSSKDKSADELTRTLVQRIAVAGLKRTIRNSRFDNPTAFI
                     DFFHFELGDDWSSLLAASKMTAKIILDGESEVTGASSDEIEILATLKEPFKALWAKCR
                     EDDSDLL"
     gene            complement(50585..50995)
                     /locus_tag="PLF14_04145"
     CDS             complement(50585..50995)
                     /locus_tag="PLF14_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70523.1"
                     /translation="MAKFYLRMTASGNARKLYVRCMLILILLYHYIYAGGHFGEWGIL
                     VSTILCAILFPFKRADKWLAGLHEEQKRFFLAALLALAICAVPHLHTTAVTLGFLLLA
                     AMFYPSCNVLSKWEDEETKKQWCKNPRTLSEYYY"
     gene            complement(51100..51480)
                     /locus_tag="PLF14_04150"
     CDS             complement(51100..51480)
                     /locus_tag="PLF14_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70524.1"
                     /translation="MRRTIYLQMLYLIDMHKSWIALDGSRSYAPRIIFKRSGALLCTF
                     RTIRLRAIYKHNGYERGTKWSISEYELDRALAVYRKRNSEVKARLKKGAFYMEAKDVN
                     AIVELATYGLLKLELSEFPLYKQR"
     gene            complement(51477..51923)
                     /locus_tag="PLF14_04155"
     CDS             complement(51477..51923)
                     /locus_tag="PLF14_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008775663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70525.1"
                     /translation="MLQLFYNDFLSFVPLQLPQLLDVTTMEQPQFYDDYVLLSFPLAE
                     QYDLEEVMDMLEDDMELIILYHHIQTGATNFGSSTCAYSNPTFGQMFKMNARVTDTGK
                     VDRIDVTIYDSLEFMCSDICLDLKLHEQGGYFKYRKNKEELLAEFI"
     gene            complement(51953..53026)
                     /locus_tag="PLF14_04160"
     CDS             complement(51953..53026)
                     /locus_tag="PLF14_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70526.1"
                     /translation="MKSERKIKGVDVISYLKKQSVLAKGIAVCFAIFILASCGKNTDD
                     SMFQTSESAVNAYRTYLSDMRSMETLSTEHLIEAVNGWQALRDSVFVRIAKDTADHIH
                     AKYETTIQMLHDSLQTEFIRLANSTPKTFKDVLLIKERTSPYRQDAELAQAVATAEPF
                     FDSLNSIPLYRKTTKNIVSVYKTFLAQTLKSGINSKDEMLDFIREEDRLFRSFLAHLP
                     ELADADLSAITGDTEQCCLSVFQSAEDGKFSYRDALIYFAKRTDRRIVFNALVCRNDI
                     NQDKVKTDAQARAYVWMLLQPYIALDDFSMAVLSDKERVSLHGVAEQMPSMIARLNKI
                     IGTDNDQWETLPELFIKTMITSI"
     gene            53288..53914
                     /locus_tag="PLF14_04165"
     CDS             53288..53914
                     /locus_tag="PLF14_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009036702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOT70527.1"
                     /translation="MAKVGYIFKAAGYDGFDTDKEWMEQYGCVQVIEEENGHEKLRPQ
                     WKQLMASLERGDELVVSKFSNALRGSRELATFIEFCRVKVVRIVSIHDKIDSRGDLFP
                     ETKASDVLEMFGSLPEECAMLRKASAHIIHLKQNINQPTKEKSLSRAEREKTIVAMYN
                     NGHSIDDIWKVSGFNSRSSVFRILNKYGVSLNRGKFSGPLGKRKPKEE"
     gene            53924..55183
                     /locus_tag="PLF14_04170"
     CDS             53924..55183
                     /locus_tag="PLF14_04170"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HOT70528.1"
                     /translation="MRIMEYLKITDSLKKEIEPKLKDYFLGRYYKTETNHYHDNRFIQ
                     LETILHDIDIHYEYYQGYVELHLEGKFSGYEYNTIWRELVEESYQHQELSWHRWGNRN
                     RGRCRINISIQSADDIIKCFEKISKIFDPVLSPLSAEHGDAEVIMQQMENLEILPPAD
                     SMQVEKLTYGILKIKELPFNNFIIPEYQRPYKWGVKNVNQLINDLLYFKDSEEYRLGT
                     LVLHENNIVDGQQRIVTLSLILYELFNKKEIRIKNPYQEVQDRIKTFWKRTEFTNEHS
                     IGHVRENLIAIEDRLDDLDDNFLDFLLSKCQFVVVRLPEISEAFQFFDSQNARGKDLE
                     PHDLLKAFHLREIKRLSEKDSDNITKWQDLDPQRLVELFLTLYRVKRWAKNNSGKEFT
                     KDNIDAFKGISLEDNGKIGACCYTWHD"
BASE COUNT        16495 a        11534 c        12339 g        14829 t
ORIGIN      
        1 attccgattc agatcaactg aatctatggg gtaatttctt tggacagtag tgaaattaga
       61 gcattatgat taacctgcaa aaaggacagc gcattgaaat tggcttgtca aaagtcggtg
      121 ttggtttagg atgggatcct aatgagagta ctggatttga ttttgattta gatgcttcag
      181 catttatgtt aggtagtaat aaaaaacttc cacaagacga gttttttgtt ttctataata
      241 atcaaaaatc accagatgga gctgtcgaat cttcaggcga tgatttgacg ggaggtaata
      301 gtgatggtgg tgatgacgaa actctaaccg tagatttgac caaagttgat ccacgtattg
      361 aggaaataat cttcacagta acaatccatg aagcaattgc aagaaaacaa aactttggac
      421 aggtacataa ttcatatatc cgaatttaca atgcagtaac taatgaagat attgcccgtt
      481 acgacttaga tgaggatttc tctattgaaa cagcagtaga atttggtcgt ttatataaac
      541 gtggttctga gtggaaattt gaagcaatgg gtattggcta taaaggtgga ctgcaatact
      601 ttgtagataa atatgcctac taatgcggtt tgtaaacaaa ataaagtttc aatgcctttg
      661 acaataaatt catcaatgac acaaaaaacg atataatatg atcaatcttg aaaaaggaca
      721 acgagtaaat gtagatttgc ccaaattcac aatcggtttg ggttgggata ccaactcatc
      781 aagtactggc gaagatttcg atttggacgc atcagtattc attcttggag aggataagaa
      841 attaatttct gatgactatt ttgtcttcta taatcagctc gtatctccag atggcgcagt
      901 tgaacacact ggagataatc tcacaggcga aggagatggc gatgatgaaa gtatcaaaat
      961 agatttaaca tctattacac cgaaagcgtc agaaattgta attgtagtta caatccacaa
     1021 agcagctgaa cgtaaacaaa acttcggtca agtccacaat tctttcatcc gtattttcaa
     1081 tacagataca caggaagaaa tcttaaaata tgagcttgaa gaggattttt ctgttgagac
     1141 agccgttgaa tttggtcgta tctataaaag aaacggccag tggaagtttg aagctatcgg
     1201 tatgggacaa aaaggtggct tacaagaata ccttaataaa taccaataaa atatcatggc
     1261 tatacgatta gagaaaggac agcgcattaa tcttgaaaag aataatggct ctaaattgac
     1321 ctcattttgt gtagggtgca actggggtgc aatcgagaaa aagacatttt ttggtctaag
     1381 caaagaaact atagatgttg atttggactt gagttgcgta atgttcaatg aacaaggaga
     1441 aatctgtgat cacatttatt ctccactata taaaccagat ttgctttcaa aatttggcct
     1501 tcctcctgga aaggtagatt ctttagatag agcattgcat cattccggag atgatttgga
     1561 aggtgacaag ggtggagacg atggacttga caacgaaatc ataactgtcg atttgaatag
     1621 attaaatcca tctatacatc agatatactt ctttttgaat aattgcggtc aagaagattt
     1681 ttctcaaatt ccttatgctg caattagaat gtatgaggga actcctacaa gagtaaagga
     1741 agtatttgcg cagtatgatg tcgctgccga atcaaaatat caaggcaaaa ctgcattgat
     1801 aatgggcaaa ttgtacaagc gtaatggcga gtggaaattt gctgctatcg gtgatgccta
     1861 tgatgataaa ttcttaggat ttaccattca tcgcattatt aaagattacg taaaaaaata
     1921 acacttatgg caatacgatt agagaaagga caaagaatca accttgaaaa aagtaatggt
     1981 tctaaattgg agaacatttg cgtaggtgtc aattggggtg ctatcaagaa aaaaaattgg
     2041 cttggattta ctacgaccga agccgttgat ttggatgcaa gttgcgctca attcgatgca
     2101 aatggaaatg ttattgacaa agtttggttc agacatctga aatcaaacga tggtgccatc
     2161 aaacatagcg gagacgattt gactggagat acagatggtg atgatggatt ggataatgaa
     2221 gtaatcacag tgaatcttaa tcaattgaat cctaaaacag aaaaaatagc ttttgtgcta
     2281 aacagtttta gaggacatga ctttaagacg ataccttttg cttcaattag aatctatgaa
     2341 ggtactccaa ctaaagtaaa agaaatcttt gcaacttatg atattgcaaa cgacccgact
     2401 tttgccggga atgtctcaat ggtaatggga gttttctaca agaggaatgg agaatggaag
     2461 ttcaatgcca ttggagaacc taccaaggac agagattttg aaagtacatt acaaactgtt
     2521 aaacaacgat atttatgaga agattacccg tttatctctt gttagatact tctggttcga
     2581 tgtatggcga accaattgaa gcagtcaaaa atggcgtaca ggtactcgta tcaacattga
     2641 gacaagaccc atacgctctg gaaactgcat acttgagcat tattacattc aacagtagtg
     2701 cgcagcaagt aacccctttg acagaactat cttctttcca acaacccaac atcgatgctg
     2761 gtggttgtac agctttgggt gaagcactgt cattgcttgc atcaaaagtt gacagtgaag
     2821 ttaccaaaac aacagcagaa gtaaaaggtg actggaaacc attggttttt attatgactg
     2881 atggagagcc aacagatgat ttgaacaaag ggctggctga attcaaaaaa cgcaaatttg
     2941 gaatggttgt agcctgcgca gctggtcaag gtgcgaatac agacaccttg aaaaagataa
     3001 cagagtgtgt agtgcaacta gatacagcag atagtgctac aatcaaatcg tttttcaaat
     3061 gggtttctgc ttcagtaagt gcaggcagta tgaaagtcga agaaacttgt caggaagttg
     3121 gtggtctgag tgaacttcca cctccacctc cagaagttaa tattgttgta taactcaaac
     3181 agataaaaat gaaaacttta caggaatata aagcagaact tttggccgat ggtatcattg
     3241 atgccaatga agttaaagaa ttggaacagt tgctttttac tgacgggaaa atagattcag
     3301 aagaagctga tttccttttt gaacttaatg acgctgtttc cggcaaagac aatgactgtt
     3361 catggaacaa attgtttatc cgagcaatcg tttcttattt attggaggat aaactttctc
     3421 caggggaaat agatgacgaa gaagccgatt ggttgtataa taaaatcaaa ggtgatgggc
     3481 aaattgacat tctcgaaaga gaattgcttt tgcaactgaa gtctcaggct aaaaacttcc
     3541 ctgctaaatt agaggaacta ttgtaatacg gtttcacata ataatggggt atttcagttg
     3601 tgaagcatat cgctttacta ttgaaatacc cattctgtaa tattcaatat cagcataaaa
     3661 agaaatagta atgattagat aaaatgagac gactacctgt atacttactt atagatacat
     3721 ctggttcaat gaaaggagaa cctattgaat cggtaaaagt tggcattgaa tctatgattg
     3781 caacattgcg tcaagatcca tacgcattgg aaactgtaaa cattagtatt ataacatacg
     3841 atagagatgt taaggttatt ctgccattaa cacctttgga agacttacag ttacccaata
     3901 taacaactcc ggattctggt ccgactcata ctggtgccgc cttggaacaa ctttgtcatc
     3961 aggttgaaca ggaagtaaca ttaagtacta ccgagagaaa gggagactgg atgccattat
     4021 tgttcattat gacagacgga catccatccg acataatggt atataacaag gttgtacctg
     4081 aggtcaagaa aaagaaattt gcaagcatca tagcttgtgc agcaggaatg aaagcaaaga
     4141 ttgaaccgtt aaaactattg acggatcaag tgtattcctt agacacaatg gatagttcca
     4201 gcttcaagac ctttttcaaa tgggtttctg atttaattga ggttggcaac aaaagtgtcg
     4261 gagctaccga tgatttaatc ttacctcctc caccggaaga agttaacgtc gtaatctaac
     4321 agcaatatga atgtaaagtt agttggaaat ttcatacagc atatagaagc cgagcttatg
     4381 tacggcgaag aattttatgc acaaaaaggc actcttatat acttagaaca aggtatagag
     4441 aaggatgttg tgctgaatgg cggcggaaat ggaactcttg gtactattgg caatttatta
     4501 ggtgcaaaac ttacaggaga atctatcttc ctcgtccgtt acgttaataa gtgcaataaa
     4561 cctcgtaaag tcgctttcgg tggaagtttt gggctgcatc cagttaagat acaaaacgaa
     4621 tccctgattt gtaatagagg tgtgtatgtg gcttccaaca acttagttca agtatctgcc
     4681 aaaatttcaa ttgcaggtat agttggcggt atgggtggac ttttacagaa aatatcagga
     4741 acatcgactg tgtttctgga ctgcaaggga caacctataa caaaggagtt atgcgcagga
     4801 gaaactattg aagttgatga agatcacatt attgccatgc aaggaatttc agagcatcaa
     4861 ctttcttcag cttggtcact aaaaaatgta ttcgggggtg aaggacttag tatgcttcgc
     4921 ataacaggcc ccggtaaagt acacttaagt cctggtagta tcatacctca aacaacaacg
     4981 aggtaatacc cacatataga ttaagaaagg cattaatatc agaaaatact aattatatga
     5041 gacgattacc tatatacttc ctcattgatg tatcggaatc aatggtaggc gaaccaatag
     5101 aagaggttca aaacggcata cggacaatca tacaagagct acgctttgac ccatacgctt
     5161 tggaaactgt atatgtatcc ataattgctt ttgcaggaaa agcaaaaaca atttcggaat
     5221 tgacagagtt gttcaaattt tatccaccta agttcccaat aggcggaggt acatcattgg
     5281 gagtcgggtt gaatcatctt atggatagta tagatcgtga tgttcaaaaa acaacattgg
     5341 aggcaaaagg cgattggaaa ccgatagtct ttctattcac tgatggtacc cctaccgata
     5401 acccagacag agccattcaa cgttggaaca ccaaatacag aaaaggctgt aacttaatag
     5461 ctatttccat tggagataat gttgatacca aaatgcttgg tagcattaca gacaatgttt
     5521 taagattgaa ggacacagat gcaaattcat ttactgcttt ctttaaatgg ataaccgcat
     5581 ctatcaaaac atcaagtgta tctgtttctg aaacagctaa tgatgaacta aaattggctc
     5641 caatagatgg tattaatctg gagaagattg atacaaataa accatgtcgc attgatgaaa
     5701 attttgctgt tgtcatggga aagtgccaaa caaccaaacg tccttaccta atcaaatacg
     5761 caaaaagaat cagaccattg gatgtagaag gattggagca ttttaacgtc acagatttta
     5821 aattagtcgg ggcattccct gtagaggaag agtcatacaa tatactggct gataaatcgc
     5881 atacaaaaag aaatatcaac acaatagaac tacttggagc acctacctgc ccatgctgcg
     5941 gaaatcaatt tggatttgta gtctgtgaat gcggtaatat attttgcgtc ggtgatgctg
     6001 aatacaacaa atgtccttgg tgtgggttag aaggtacatt gggagaaggg aaagctgagg
     6061 gtatgaatgt aaacagagca agaggataat aatatgacta gtataattga tcaattaatt
     6121 gagacgtact ttcctcaagg agtatcttca gaaaagcgtt ctgacataca aaaattcttt
     6181 ctggaaaaaa tatataacga atacaacgag tactatatgg atagagaatt ggttataaaa
     6241 aatcggattg atgccttaaa tttgacgtta cccaatgcta aggtcagaga ggaatactct
     6301 caactgataa aaatagatga tcctgatgtc tgtgaatact ggttggaagg attagaagat
     6361 accggcttgc aagcagaatt ggaaatcatc ttgaatccgg cagaagaaaa gacgattcca
     6421 aatgattgtg cttctgaatc agctatgccg tctaatgaag acaatgtacc aatcgaagtc
     6481 accgattcta caaacctcaa tgaagtagca gaagtagaaa aaacagatgc gcaagtactt
     6541 tccgacacaa gaattgttgg tactaaagag cgtggaatcg tggttaaagg aatcccgacg
     6601 aaagctggtg attatgagat taccctaaaa tataaatatc agaactggag cgaaggatat
     6661 agtattttgg aacgaaaatt caaagtcgca gtaaatccag acccacgctc gctttggaaa
     6721 gacattccga cagacaagaa catcaaattt ttcaaggagg actattcaca cgaatacata
     6781 acagtcgtgg aaaatgaaaa aggtcctcaa aaggatatag ttgcagcaag taaacgtgga
     6841 cgttcacacg cccatgaagg gaaagcacgt gatgatgact ttaatatcta tcataatgat
     6901 agtaatggct ggtacattat agctgttgca gacggtgccg gttctgctaa atattcacgg
     6961 aaaggctctg cggtggcttg tgaaacctgt gttgagttct gcaaaacggc attggagaat
     7021 cctattgaat tggaaaaaga gatcattgca ttaaatagca caacagaagg tcaatcaaac
     7081 agagctatca gcacactcat ttacaacata gtaggaggtg cagcccataa agcacacaga
     7141 gccatacttg aaaccgccag tgctaacgaa gatcaaccac gtgattattc aacaacgcta
     7201 ttacttgcca tctgtaagaa gtttgatttc ggctggtttg ttgcttcttt ttgggttggt
     7261 gatggggcta tgtgcattta cgacaaggaa cgccaatata ttaaattatt gggaactcct
     7321 gacggaggcg aatatgcagg tcagacacgc ttcctgacaa tgaaagaaat tttcactccg
     7381 gaatcaatta tgtctcgatt gaagtattct atcgaggagg atttttctgc tctattattg
     7441 atgacagatg gtatttcaga tccattcttt gaaacagatg ccaatcttaa caaagtagaa
     7501 aaatggaatg aattgtgggt caatattaac tcagaggttg atttaactga tgataataca
     7561 gagtctcaat atcaattatt gaaatggttg gatttctggt cgccggggaa tcatgatgat
     7621 agaaccatag ctattctata ttaaaaaagg gaagatatga gcaagactat tacattgaca
     7681 gcgaccgatg gtacgaaagt cgaatttata gatgatgtta ttgggtctgg agccatgaaa
     7741 gatgtgtatt tcagcccgga caagagctac gttgttggat tttttagaga accgcaagat
     7801 gcaaattcta aagaccggct aacaaatata acaggaacat accgggagag aatattcaat
     7861 caagttggag gtgagtattg gaagaatctc ttttgctggc caacaaaaat ggttgaatgg
     7921 aatggcaaaa taggaatcgt atgccctact tatcataaac actttttctt cggctctggg
     7981 aatttcaaag gaaaagaaaa agaaggaaaa tggttcgctt ctgctaaatt gagaaatcgt
     8041 tttcttccag cagaagaaaa aggaacttgg cttacacatt atcatatgtg tattcaaata
     8101 gctcgtgccg taaagagatt acatgccgct ggtttggcgc attcagattt gtcgtataaa
     8161 aatgtacttg ttgacccagt gacaggtaaa gcctcaatca ttgatattga tggattagtg
     8221 gtccctggta aatatccacc agatgttgtt ggtacacctg atttcatcgc accggaagtg
     8281 cttgaaacaa gaagtttgaa aattggagac ccaaataaaa aactaccaag tattcaaacg
     8341 gacagacatg ctttagctgt actcatatac atgtatttgc tataccgtca cccgcttaga
     8401 ggaggtaagg taaatgatct ggatgctgct aaagacgaag aattgtctat gggttccaag
     8461 gcattgttca ttgaacatcc gtcagacaag agtaatcgtc ctaaagtcga tcagttatca
     8521 aaaagtgaac taccccaagg tgatgtcacc aaacttccat atagcatatg tggcccatat
     8581 atgaaagaac tgtttaatag agcttttata gatggtctgc acaatcctac tttacgacct
     8641 actgctgatg aatgggaaca ggcattgatt aagacaactg atttaatgca gccttgtcaa
     8701 aaccctaatt gtgaagtaaa atggtttgtg ttcgataact ctacaaaacc gagatgtcca
     8761 ttttgtgggc aggaatatca tggtcaactg cctgtgctga acttgtatta ttcccctaaa
     8821 aaaggagtat tcaagcctga aaattatcgt cttatggtct ataacaaaca gacattatac
     8881 aaatggcatg taaaccgttt tgttactcca aatgaaaaga caagcgatga agataaaaag
     8941 cctgttggtg atttccattt tttcaatggc aaatggattc ttattaatcg tcgtttggat
     9001 agtctgtacg ataaagactt agataagaaa atagaaattg gtcaatatgt agaattaacc
     9061 gaaggcaaaa aaattctctt gtctacagag gatggcggac ggctgattat tgttcaactg
     9121 gttaataatt aattgtcact atgataaaag aacaatgtga acaatgcaag aggaataaca
     9181 ccgattgtca agagaatata gaatttaacg gtttatcatg tgagcaatat gctaaacgta
     9241 taaatctgga gaaatctgag aagaatgaaa ctccagatga tagtaacgtt atacagcaaa
     9301 atcaagagac atctgctacc atagaatatc cagaccccaa ctatagtatt catggctggc
     9361 ttactttctt cttgttctcg ataggacttg gcggattaat aagtgcgctt taccctattt
     9421 tcacatataa cattgcagaa tacgatggaa accattttct ggccatatca gatgtggttt
     9481 taggagtaat gttgctcatt ttagcctgtt acacaatata ttccttttgc aagaggaaac
     9541 ccaatgcggt attcttagcg aagatgtatg ttgtggtaac atttataact aatatattag
     9601 cattaataag cggagagttt gaagaaacgg gactcggtag tttgccacgt attgtcagaa
     9661 gtcttgtttg gggtgttatc tggttctcat atctgactta ttctgaacag gtaaaagata
     9721 taatacccaa gtcataccgt aaggtcttta gccgggatta ttatttgatg acggcattta
     9781 tcattattcc aatattatgc cttgccatag gtgttggaaa tatattttct caaagagaag
     9841 agcaggaaca aaactttata tcaaccacgg atcttgatta caatgaatat acagatggtc
     9901 gtataatatt cactaaacca gacggtttta cttgcgaaaa gcaagaatta gacgatccaa
     9961 aaatcactgt ttttgattta gaatcagaat atagctattt acgcattgtt agcgactatg
    10021 attcagacgt ttctgtacag aatttcaata cttattggaa tggctggaag gatgatgatt
    10081 taaaggatta ttcgtataag gaaatcacaa acgaaaaaag agaaattaat ggtaatccat
    10141 acttcataaa atcagtgaaa tacgatacag aaacgcctat tatatggcat ttcattttgt
    10201 tgtttagtcc taaaacaggt aaagtttgtg tcattcattc catacaaata caggatgaaa
    10261 cgctagatat tatcgacttt atcagaacta ttcgtttttg agatatgata tacgtaaaaa
    10321 ggaataatca agagtttggc ccctatgatg aatcgaccct tttgacgtat gtcaattccg
    10381 gacagattct cagaagtgat tctgctagaa acgatgcaac gaatgaagaa aatacggtaa
    10441 agtattttct gaaaaaggca ggattaaaac ccaaagtacc tcataaagga catctatttc
    10501 accagttagc gatgattggt ggcgaattaa tattaccaaa atccaccatt atcagcaaac
    10561 aatggctttc agataaaagg ctgttacttt tagctttaat aggtctattc cctaccattt
    10621 tcatggcaat acccttccct gcaatgggaa tattttatgc tattgcactg tatttttcat
    10681 gtatatgggg aatattcttt tactattttt ttagaaccaa gcaagtgtcg ttgaaatata
    10741 cattaattgt attttttatg acgcaactct gtatattctt cctatgggat atcgtaggtt
    10801 ggacaaaact taatccattt tacaaccttg taaatctacc attcccattt aatttcatcg
    10861 gatacacttt aggagttgga acaacagaag aatttgcaaa agcgatacct ctttatattc
    10921 ttattgcccg agcaaaagag ccattaatac cacaaacatt agtgttttat ggtttaatgg
    10981 ctggtattgc atttggtgta tatgagggag ttggatatca aacttcagtt aatactcagc
    11041 ttgattatac cggagcattt ttcatgaaca ttgcacgatt gaccagtttg ccgttccttc
    11101 atgcaatatg gtgcggtatc ggaggatatt ttattgcttt cgccaattta tatccgaaat
    11161 atcgtatatc attgtacttc ctatcattag ctattccagc tttgttgcat gggctatatg
    11221 atacgttgtg tggttatagc gtattgatat ttatcgctgt tggcatatgc tttatcgctg
    11281 tattgttgct catgacttac ttaaagcaag gagtaaatta tcagtcaaaa cttagaaact
    11341 aatatactat gggaaaatgt ctatactgcg gtcaggatgc tggtttcttt cgtaacaaac
    11401 acaatgaatg cgaagcaaaa catcttaaag gtttgtcttt tattaaacaa attataatag
    11461 attgttttac acataaggag gatttttatc tgaaagattc agaaataagg aaaatcgttc
    11521 aagattcgca tatcaacaaa acgtctttag acaatatgtt ttgttctgca tttgatgaag
    11581 ctattgacgg ctatcttaat gatgggatag ttgattcagc agaagagaaa actgttgcca
    11641 gattcattca atattccggg ctttcacaat cattcctcaa tgccaataaa tctcttgaaa
    11701 aggtcttgca gtcaaaagtc cttcgtgata ttttatcagg aaatattcca acacctcaaa
    11761 taaccattgc aggtgatttt ccttttctgc tgaacaaatc agagcattta gtatggctat
    11821 tccgcaatat cacactttac caacaaaaga ttcaacgaga atatgtagga cgaagtagag
    11881 gtgttagcgt tagagtaatg aaaggcgttt attatcgcac gggtggtttc aaggggcatc
    11941 ctgttgaaac aaccgttatg caaaaaattg gttctggaag tgtttgcctc accgataaac
    12001 atatctattt tgcttctcct gaaaaaagtc tgaaaatacc atactcaaag attttgagta
    12061 ttgaatcata ttcaaatggg ataggattgc aaaaggatgg ggctaatgac aagcctatat
    12121 tccttgaagg aattaacagc tggttcgctt ataatgttgt tgcaaattac aaataatcgt
    12181 tatgtataat ttagaacaga ttaaacaaac tatcatagca aacggaagca tcacttcaag
    12241 tgatattcca gacttgcgca agattgtata taaagatggt cgagtaagcc gagaagaggc
    12301 tgatttttta tttgacttac gaaaagagat actaaatttt ggcaatctaa aagaatggga
    12361 taattttttc attcaggcca tttgtgacca tctattagaa gaatcacatg acacaataac
    12421 tgacgatgat gtatcttggc tcatagacaa aatcggtgcg gataaatata ttgaccatat
    12481 agaacaagag ctactttgca agttgagttc taaagtgaag tatttcccat caaacctaaa
    12541 ggcaattcaa gaacatacaa acattgcata tgatcttggg tgcaaaatac tgtcattatt
    12601 gtgcagaaat tcaaaaacag gtcgtagtct tcaattcggt ataaataaag tcggttgtag
    12661 aaaaaacaaa atagtagtct aaacatctat atgcgataca tatgattagt tgaatttgta
    12721 gtgtcgttat catcgcagta ttacgcataa gtcacatttg aaaaatatta ttaaatagaa
    12781 tcattatatg gaacaaaaga aaaattacat tggcttaact gatgcccaag tgctagaaag
    12841 ccggaagaag tatggagcaa acattcttac tccaccagaa aaagatccgt ggtggaaaga
    12901 atttcttgaa aaattctcag acccgttaat catcattctc cttgttgccg gagtgctttc
    12961 aatcggaatt tcattctatg agttctttgg cttagggcaa aattggaaag ttttttttga
    13021 gccaataggt atcttcgttg ccataggatt ggcaacaact cttgctttca tctttgaaca
    13081 aagggcaaac aaggctttcc aaatactcaa tcaggtcaac gatgatgaat tggttgaagt
    13141 tatgagaaac ggtacgacta ctacaattcc tcgaaaagat gtagttgtag gtgatattgt
    13201 catcattaac acaggtgatg aaatacctgc cgatggtgaa cttcttgatt ctgttactct
    13261 cagtgtagat gagtcaacgc ttacaggtga gcctctttgc gccaaaacca tcgacccagc
    13321 gcatttcgac aaagaagcaa cctatccgtc caatcatgtc atgcgtggta caaaagtaat
    13381 ggaaggtcac ggcgtgatgc gtgtgctgaa agtcggtgat gcaacagaga tgggtaaagt
    13441 atttgaggaa gcgcaaattg acgattctgt aaagacaccg ctcaatgaac aacttgacgg
    13501 actcgcagat tggattacga atgtcagcta tggttttgct ggattgataa ttgttggtca
    13561 gttgattcat ttcctcggat ggagtaactg gcaagcatgg acgcttattg ttcctgttgc
    13621 tttattcttt tggcttgtca tcaaaaaatt tgaagattgg tcaaaggcta agtgcatatt
    13681 tactatcatc ggatttttcg tactgttctt cgctatggta attggagcgt tctcaatgat
    13741 acacgctggc gcagatagta ccacttggtc gttgctcctt gctcatacac ttaaaaccct
    13801 catggtggct gtaaccttaa ttgtggttgc cgtaccggaa ggattaccta tggctgtaac
    13861 cctttcactt gcatatagca tgagccgtat gcttaaaacc aacaatcttg tccgcaagat
    13921 gcacgcttgt gaaacaatgg gtgctacaac cataatttgc acagacaaga caggtacact
    13981 gacgcagaac cagatgcagg tctataaaac taatttcttt ggagagcctt cagatgaaat
    14041 tctttatgag ggtatagctg taaactcaac ggctcaactt gatttgaccg gagataagcc
    14101 acaggttttt ggcaatccga ccgaaggagc gttgcttctt tggctgaagg aacacaatac
    14161 agattacaag gctttgcgta tgaatgccac gcgtattgaa gaactgccat ttactactga
    14221 acgtaaatat atggctaccg tggtgaaatc tgcgacaggc aagaacattc tttatataaa
    14281 aggtgcgccg gaaattattt tcgccatgtg taagaatact tgcggcgttt ccaaacaaga
    14341 gatagacgct cagctgcttg catatcaaaa tcaagcgatg cgtactctcg gttttgcata
    14401 tcaggaactt ggagataagg atgctactat tgaaaatggc aagatcgtag ccgataaact
    14461 cacattcctc ggaattgttg ctatctctga ccctgtacgt ttagatgtac ctgatgccgt
    14521 taatgaagtg attgcagccg gaatcaaagt taaaatcgta actggtgaca cacctggaac
    14581 ggcaaaggaa atcggtagac agatcgggct ttggaatgat gcgacggaca cagaccgcaa
    14641 cattattact ggagttgagt tttcagaact gtcagatgcg cagttgcgcg atcgtgtagg
    14701 cgacttgaaa atcatagccc gtgcccgtcc tatggataag aagcgtcttg tagaagcact
    14761 gcaaacgaac aatgaagttg ttgctgttac cggtgatgga acgaatgatg ccccggctct
    14821 gaagacagct catgtgggct tgtctatggg agatggaaca tctgttgcta aagaagcatc
    14881 tgatattacg attatagaca actctttctc gtcaatcggc cgtgctgtaa tgtggggacg
    14941 ttcactttat aagaacattc aacgcttcct attattccag cttacggtta atgtggcagc
    15001 ttgcttcctt gtactgtttg gctcatttat gggtaccgaa tctccattga ctgttactca
    15061 gatgctttgg gtaaacctta ttatggatac attcggagca atggcactcg cttcgctacc
    15121 cccttctgca agtgtgatga atgataaacc gcgtcgtcgt gaggcttcaa tcttgactcg
    15181 tccaatgatg acagaactac ttggtgtggg tttgttcttc tttgccttga cattgggctt
    15241 ctactggttg ttcaaccatg ctgaagtaac ctcgattaca cagatgttct cagcagttgt
    15301 aggtggtgaa aatccgatga cagcatacga agctacactt ctcttctcca tttttgtatg
    15361 gacacacttc tggtatatgt ttgatgcccg tgtatttgaa acaggagaaa gtattttcaa
    15421 agtaaaaatg agtcgcggat tttggactat agtcggaatt atcattattg gtcagctatt
    15481 tatcactgag attgcatacg aattctttaa tgtagaaccg atgcttcaca cccttgattg
    15541 gcacttcaat cccacaggtg ccattgactt gttgataatt gttagtgcat cttcactcgt
    15601 tctttgggtt cgagaaattt gtcgattgtt tacaaaaaaa taatacgtaa ttctatgggt
    15661 gaatgagatt caaattcacc catagaatta tctcacttat tgtgtatgaa aagaataatt
    15721 atcacattgt ttgttgttat aggaatgact tgttcgattt ccgcccaaaa catcaaacgt
    15781 ccggattctt acaactacaa tcgtggcgtt gaagctattc aaaacaacaa tgcagaagag
    15841 gctcttgaat accttaacaa ggagctggaa gataatccaa acaacggata tgctttggct
    15901 tggattgcga ctgtacgaaa ctatcaagag gagtatggtc gcgcattaac cgctgtagat
    15961 ttggcaataa agcatattcc taaaaaggac aaacaatata gagcctttgc atatattgtt
    16021 cgtgcagaag tttatgtggg acttggtgaa aatgaaaaag cacttgtcga tttcacatcc
    16081 gccataaaag aaactcctga tgctgctgat gtctatgaaa agcgagccaa tctatattat
    16141 tatatggata aatatgactt ggcagataag gattaccaga aaatcatatc aatagatcct
    16201 ggtagtgtaa tgggctacat gggaattggt cgaaatgcca atgcggaaaa aagatataat
    16261 gacgcaatcg agcaattcaa ttatgtgaca cggttggcat cagactattc ttcagggtat
    16321 tcttttaggg ctgaaagtta ttttgggcta caacaatacg acaaagctat tgatgatgtt
    16381 atcaaagcgt tagatattga cggtgacaat aaagcatttc atttaatgca gcaagtcgca
    16441 gattccgcta tgattccgtt agtagcaaaa ttaaaggtgc aatccgcaaa aaatcccaat
    16501 aatgaatatt ggctctattg tttgggaatt gtacacgaac gtactgatgc ttataaaaag
    16561 gccattgaat attatacaag ttgctataat aaagatgctt ccgatgtagt cgcctatcgc
    16621 atatcacaat gccatagcga aatggggaac ttttccttgg ccttggatca tattgataat
    16681 gcgattgcct tggattctac agactttgat tatgtgatgg aaaaagcaga tcttttgtat
    16741 gaatccggga atacgaaaga cgctatcgtt gagtcgggta aatatatcaa ccattatcct
    16801 gattattttg gagggtacta tcgtcgtggc tggttcaagg acaatagcaa cgacacagac
    16861 ggagccattg aggattatac aatggctata gttttgcagc cagattatgc atatgcatat
    16921 ttgggacgag gtgatatgta catcctgaaa ggagataaaa aatccgcaga agcagactat
    16981 agaaaagtta tagaactcga tactattcca agtaatagtt cttgcgctca atacgcatat
    17041 tggggattag gccaaaaaga aaaagccatt gaatttatgg acaaggtgat tgccaatgat
    17101 tcggataatg ccggtaacta ttatgatgca gcttgtttgc attcaagaat gggcgaatta
    17161 gaaaaggcta ttgaatttat gcgaacggca ttggaaaaag gttatcgccg ttttgctcat
    17221 cttgaaatgg atgacgacct aaatccgata agagaattgc cagagttcaa ggaaatgata
    17281 gcgcaatata agaaacggtt tgaatcagaa atagaaggca agacacaaga tacatcccta
    17341 tatgaggaaa agactgttga aatacctttt accaaagaag acggtgtctg taaggtaaaa
    17401 tgtgcaataa acaatctgtc tttatacttt gtttttgata caggggcgtc agatgtatct
    17461 ctctcctctg tcgaggctac attcatgatg aaaaatggct atttgaaacc aacggacgta
    17521 ataggcaaac agaattatat gatggccaac ggagaagtaa gtgccggtac ggttgtcaat
    17581 ctccgtaatg tgaatttcgg tggtttagat ttggataatg tccgtgcctc agtcgtgcat
    17641 aaccagaaag cccctcttct gttagggcag tccattctca accgtttggg aaatattaag
    17701 attgataatg caaatagagt gttgaagata acgtataaaa agaaagtaga ggaaaacaaa
    17761 tgaagcgaga aatcaatagg ttaaaagtag ttttggctga gaaaaagcga accaatcgtt
    17821 ggcttgcggc tcaattagga aagaatgaag ccaccatatc caaatggtgt actaataaga
    17881 gtcagccaag tttggatgat ttagtcgcca tcgcaaggtg tttggaagtc gatacaaaag
    17941 atttactcca ttcgataact gagtaattaa cgtagatacc acctattttg gggtggtatt
    18001 ttactttagt accaaaataa cttccaaaga atgaagtgtt tggattttaa aaatcactat
    18061 ttttgtataa agatttaata cactaccgat atgaactcag caaaatacga agaatatatt
    18121 tcaaaataca atttaaggcg gttcgttgat gcgcatcgcc agatatacgg ctctgccttg
    18181 acggaaattc gttctggcaa gaaacaatcg cattggatgt ggtatatatt tccacaactt
    18241 cgaggtcttg ggcatagtcg caatgctgaa tattatggta ttatagaccg cgatgaagca
    18301 attatgtttt tacttcatcc tctgttaggc agaaacctct acgaaatcac aatggcaatg
    18361 cttaccattg acggaaaatc cgccgaggaa attttaggtg gaatagatgc cttgaaattc
    18421 cgctcatcaa tgacgctttt cgataacata tgtcctaacg atattttctc tgatgcctta
    18481 cagaagtatt acgatggtaa atctgatgaa cgcacattgg aaatgttgca aacaagtaaa
    18541 gtcaaggacg aacagcttat ttcaggggga attattggtg ctataatcgg agatattgtc
    18601 ggctcatttt atgagttttg caatcacaag tcaaccaata tcgctttatt tacgagggga
    18661 actcagttta cagacgatac agtaatgacc attgctgtag ctgactggct tttgagtgga
    18721 gttccattgc gggaagctat gctcgattgg ggtaaagaat atcctaacag agaatatggc
    18781 ggaatgttct atgagtggtt gttttatagt gagaccaaag aaccatacaa tagttttgga
    18841 aatggtgccg gtatgcgtgt aagtccttgt ggatattatg ccacatcgct tgacaaagca
    18901 cttgaacttg ccaaacaatc agcagaggtt acacacaatc atcctgaagg aatcaagggc
    18961 gcacaggcta ttgcttcagc aatcttcttg gctcgccagc ataaatccaa agatgaaatt
    19021 cgggattata tcgaacagac ctttggctat aatatgcatc gatcttgcga tgagattcgc
    19081 ccgacatatc agtttgacgt aacttgtcaa ggctcctgtc cggaagcgat aattgcattc
    19141 ctggatagtc acgattacga atctgctatc cggcttgcag tatccttggg tggtgacagt
    19201 gatacaatcg cctgcatgac aggcggcatt gccgctgcct attatggcat cccctcatgg
    19261 cttgttaagt atgttacggt tgagtttctg cctcagtata tccgggatat aatagcgaaa
    19321 tttgataaag tgtgtgcaga acgcattgct atacaatcga aatttgacaa taaagaaacg
    19381 aatagataac acaaatcgaa ctcttttgaa aaagtattat tgtttatgca ggatgatatc
    19441 atagtcaaat tgattgtaat aacatggagg atttgtagat ggttcttttt acaaatcatt
    19501 ctcatgttct tgatgccata tttgtgggct gaccactata atggcgagac aatttgttta
    19561 atgctacttt acacagcttt atatgcaata tactgggaaa ccgtaccgaa agataaaaga
    19621 ttacgttggc tgtttttacc atatttcatg tattccggca tagccatttt gttttgtggc
    19681 ttgattaaaa cttggaatgt atctgtatgg tattcctttt tattgcccat atatggcgca
    19741 acttgtgtaa ttcttgcgaa aatttgtgga aagcagatta gaagaattcg gaagaaatac
    19801 aaatttggca aaataataac atatactgca gttctcttgt tttttgtcgc gctgaaatcc
    19861 atctccgttt catgggaatg ttcaaaacga aattcttttg aaaaggaaga cattttggcg
    19921 aggaaagatt acttactggg taaattgatg gtaaatccac aggtagtcct gaacgaaatg
    19981 cctgctgcaa taggaacaca atttcaggga gagtgggcat tgtattcttg ttccatgttg
    20041 tctgcggcat tggtaaacat atcccaatta tatccagaga caaaaaccgg gaacattcaa
    20101 ctcattgaca gtctcatcaa tattgtaatg tctcccgaac ttcgctatta tgatacaatg
    20161 cgatggaatg aagatccatt ggaatctttg gacggcgatg aaagccatat atcctatctc
    20221 agtcacttag cttggatgat ttgcggctac aaacaaatcg gaggtaattc aaagtacgat
    20281 aagttattgt tttctttgtg tagtacaatg aatagaaggc ttcttaatcg aagttccttg
    20341 aatttgccca cttaccccgg agagtcaatc tacattccgg atatgcttgt tgccattgtc
    20401 gctctaaatc aatatgcaga catgaataat ggtaaatacc gtagcactgt caaaaaatgg
    20461 ataaaacgag cacaaaaaga atggattgat gacaaaaccg gtttgttggt ttcattcctt
    20521 caggagaatg gaaaccagta cgaagaagtt ccagttaaag gttcatattc agctttaaac
    20581 tgctactatc tgacttttat agatgaaact tttgccaagc aacaatatga aacgctcaaa
    20641 tcattgtttt ggaaagacgg tgttatttcc ggactaaaag aatactatga cagaacctgc
    20701 tatttggggt tggatataga tgccggacca attttatttg aattaagtcc atcaggcact
    20761 gcattcatga ctggagctgc gacctatttt aatgatttaa aaacgagaaa tgatattttg
    20821 acaacagctg agattgcagg tcataccgtt aggctaaatt gtaaacgtca ttatcttttg
    20881 gctaatgtag ctttagttgg tgaagctatt atgttggcta tgcgaacaca tttcaaataa
    20941 tatttttgac aaactccatc acacaagact ctttaacaga gttgatataa tcaaggaaaa
    21001 tcctgggatt gacatattct cctttttgac gtatggtaat gtgcaaatgc tccccagtgc
    21061 tgcgccctgt gttaccggtg attccaacaa catcacccga cagaacgtcc tgcccctgtg
    21121 atacgaaaat ttgagacagg tggcaatagc tgacggtgaa gctgccgtgt tgcaacgtga
    21181 catattttcc ggatactttg tcctgtccta ccttcaacac tctcccctgc atcatggcaa
    21241 ataccttgga actccgggca tggaggtcaa tgccgttgtg aaacttcctc ttccctgtaa
    21301 aagggtcttt ccgatagccg tagggcgaat tgattttgac agacgagagc ggatagcata
    21361 cgctgagata gcatgctacc tgctctttct ttttgatgtc cgtacttgac aaagcaacct
    21421 tgtcgggtac ggacttttcg ttttcagagg tgatgctttc gggtgtcgcc tctgtttcgt
    21481 ttgtcgtttg agatggattt tccacaacga ctttatagcg attgcccgga caggaaatcg
    21541 tgttgaactg cgctttcata tgtttcccgg acaatagcaa aagaagggtt ataaggattc
    21601 gtgttttact catagatgca aatgtaggaa cagttttccg tccacgccaa aagatgtttt
    21661 gatttacgat taaaatataa cattaaataa gttcagagta tgattatcat actataatca
    21721 tacttaaaac ataaataaac acaaataatc cggctatttg aagcaataaa aattgaagta
    21781 tgaataatag tatatacctt tgccaaagta taattaaaat atcatatcaa aatatgaaac
    21841 aagtacgatt tgaatttgag gaactgccat acgacaccct gtcgcagttc ggacttaccc
    21901 aagaaatgat tgaagacctg cccatgcaga ccttggaggc aatcagtcac ggcaaacact
    21961 ctcccgtact gcccatcaag gtaaacgatg agaacggaaa caccgttacc gatcgaagcc
    22021 gtttcgcttt cgtgcgcaag gaaaacggag aagccgatgt gatattcttc ccggtattga
    22081 agaaatcgcc tttggagaaa tacaacgagg agcagaaaaa gcagctccaa tccggaaaga
    22141 ccatccttgc agactccgta acggcggacg gccgcaagac caaggtattc gtgcagatcg
    22201 acaccgagac caaccaggtc atgtccgttc ccacacaggt catcgcccgc aaccttcagg
    22261 tattggcaga ggaactgaaa ctcagcaatg ccgaaatcaa ggtgatgcag caaggagaac
    22321 ctttgacctt cgtgatggaa gacgaaccgg ttacggtcgg catagacctg aacgagaaga
    22381 acggcatccg cttctgccag ggggactgcc agaaatggag cgagcacacc aaaagagaat
    22441 gggacaaata cactttcggc tgttacggct gttgggtcat ggacgatgac ggatgtcttg
    22501 actatgtacc cgaagaacag tacacggaag aactctggaa cgaacagaaa aagagtgccc
    22561 agcgcaatat gggcgcagga cttcacaaat aaccttcaat ccttatgatt atggcacaaa
    22621 acgcatttta ccccgaagac gttctcatcg agaagatgga gagcggagaa tacggctggc
    22681 tggattatgt caaccacttt tctgccgaat ggcaggatga actggcggaa tactgcacgg
    22741 aacgcgcatt ggaaatcaac gatgcctctg ccgagcagtt cgtccattac aaaagcgaac
    22801 aattagaagc cgccatggag agcggtgaag catagaaatt atggctatac gaacccatac
    22861 aaatccaagg atagcggact tgcaacctga aatgcgtgac ctgttgatgc gcaacggtat
    22921 gcaagcccac atcgtgcctg ccggagaggg ctaccagttg gtagtacaag ggcacgactc
    22981 tccgctctta cgatacccca tcagcaagca gcagttttca gctcttaccg actggggtac
    23041 gaaccatgcc aacaaaaagg catacaacac ctttacggag atagtaaagg atgactttga
    23101 cctgccacgc aacttcgtcc atgcccgtaa tgccaacggc agggtggcta tgggactgca
    23161 cggctaccgt atcggcatcg gggaatacgg cagggtcggc agatatggga tgccgcctcc
    23221 attcttggga tggacacccc gacagcagga aggttaccac ctccgcagag tgggcggccg
    23281 gcttttctat gctggaacgc ccatggtggc agaacgtccc ggcggacgca tgaaacccgg
    23341 agaactccag tcgggcggtt acggattcta ttacaaaggc catccacaac aaccggcaca
    23401 acccgtccgc caagacgtgt tgcaggagct gcaagccgtc atcaccccga tggtaggcaa
    23461 gcccagaagc gaagaacctg caaagcccta caaggagctg attacctccc ctgtctattt
    23521 ctctaatgag aagtggcagg aatgcctgtc ctcacacggc atactcgtag acaaagaaga
    23581 aaagaccctg accatacaat cggaacaaat ccatgctgat atggtctatg acatcacaga
    23641 tgcggaatta tccgcactga cctccaattc catcgaagag cacccgatag agcgacgatt
    23701 ggagctgttg aacacggtta ttcaggaaga cttcgccgat aagatcacga tggattccct
    23761 gaatcatacc gagcgcatcg gtatcacctt gcatcccgaa gtagagcaag atttggcggc
    23821 acgccaacag caggaacagg aaatactcct gcccttggaa gctgataacg gacttgcacc
    23881 atattcaaac gaacaggcca tattgcaaga agatgaatcc attatcagag agccacagga
    23941 aggggctttg attgacggac gagacttgcc gtatctccat gaaagcaagg gatggtatcg
    24001 ggaaggagaa cacggacggg aggtggaagt ggaagcgatt gccgtacagt cggcagaagc
    24061 ggaaggcaaa tacaggatga ccgccattat caatggagaa gccatcaccc aagaaatcag
    24121 ccaaaagcag tacgacaagt tcctggcagt ggatgactac caccgcatga agctcttctc
    24181 aaaagtgttc aacgaggtag atatgaagac acgcccggag atgcggagta acttgggtgc
    24241 aaagatattc gcagcccttg ccgcaggaac agtcgtggct tccgaccttg tccacggcat
    24301 tcaccaccac ccctgcccgg aattatacgg cgagcatttc tgtcatgcgc cccgtcccta
    24361 tttcaagccc ggagtggatt caccgaggga tgtagccgcg cggaacttcg aggcacagat
    24421 gaaccaagaa atagcggaga tacgaagagg attttaacta atatgataca cagatggcaa
    24481 acggagaact gacatacgat gacttcctgc aacgcttgga catacaggac gtactggtgg
    24541 atgcaggcta tcacctgaac aagcgtgacg gcctgcgtta tccgtcctat atccgcacgg
    24601 acagcaatgg cacacgcatc cgtggagaca agtttatcgt gaccggaggt ggcaaatgtt
    24661 gcttccagcc cccacagcaa aagctctaca acatcatctc ctttatcaag tcgttccccg
    24721 aaaagtttgc ggaacacaga aacggcgtgt ctcccgacag gttggtcaac cttgtctgta
    24781 accgactgtt gaaccacccc atcgaaaaca gaccaatctg tatcatccaa cccaagcagc
    24841 acagccgccc gttcagtctc caagattacg acatacacca ttttgacgta aacgaccggg
    24901 agacccacaa gcgtttctat ccctacttca agaaccgtgg cattgacatc ttcacgcaac
    24961 gtgcttttgc cggccatttt ttcttagcca ccaaacacag gacagacggt ctgtcatacg
    25021 ccaacctcgc cttcccgttg gtattgccca aagaaccaga caaagttgtc ggtttagaag
    25081 aacgtggacg tcccaagatg gacggcagcg gcagctacaa gggcaaagcc gagggcagca
    25141 acagcagcga aggcttgtgg atagccaatt tcagcggaga acctttgcat caagccggag
    25201 gtgtcgcctg gtttgaaagc ggctacgatg cgatggcatt ccaccaactg caccgtgaca
    25261 ccttccgtga caatcccgaa ctttccaaga aaagcgtgtt tgtctccacc ggcggcacgc
    25321 ccaccgatat gcagatacgg ggaatgctct ccgccacccc gaatgtgaac cactacctgt
    25381 gtttcgacaa cgacagtgcc gggcgcgagt ttgtaaataa attccggctc atcgcaaaat
    25441 ccatgcacat caatccggaa cagataagag aaatcccctt gatgccctgt tacaaggact
    25501 ggaacgatgc cctgcttggc aagacgagcg aggaatacct tgacagcatc aaagatgcgg
    25561 ttattccttt gagtgcgcca ctcggtacaa aaggaagcac agccggcgaa gaagaagtaa
    25621 acgaacaatc caccatccac agataagagc catgcagaac agacagctat acagagacat
    25681 ttcgcttcgt ccgaaaacca cgcagttctt cattgatgag ctgcctttgt tgtttgtggc
    25741 tataacaggc ttggtctatg gcggtatgga tgacgcgccc cttggctcta tcgccaccct
    25801 ctttgccgtg cttctttccc tctacttggc ctaccagctc atctatctga aacggatacg
    25861 ctaccacatc gggagcgaac aactgacggc agcgcatggc gtgttccaac gcagcacagg
    25921 ttatatcgaa ctgtaccgag tagtggattt tcacgaacac cagaccctgt tacaacagat
    25981 tttcggactg aaaacggtaa cggtactctc tatggacagg accacaccca agctggctct
    26041 gattggcttg cccaaacaga cagccatcgt ggaacttatc agggaacgtg ttgagtttaa
    26101 caaaaaaaga aaaggcatct atgagatcac caatcattaa aatactggtt ctgccactcg
    26161 tcctgttgct gacccacacg gcaaaggcgc agattgtaac tgccaaccct ttggagtacg
    26221 tggcattggc agaaggcaac gagctgattc tcgccaaggt cagagaccag attgacggtc
    26281 agaaaaagac cgcccttttg cagaacacca ttgccgcaga gttcgagcag atgcgccagt
    26341 gggaaaagaa atacaacagc tatctcaaaa cagcaagcgg attcgcctct tccatcaaag
    26401 cctgcaccca tctgtacaat gacggcgtga ggattttcat cacactgggc aagttgaaga
    26461 atgccgtaag cgacaatccg caaggcattg tggcttccat tagcatgaac aatctctata
    26521 tagagacagc gaccgaactt gtaaccgtat tctccctact ccgtgatgcc gtggccaagg
    26581 gcggcaagga gaatatgctc acgggagcgg aacgtagcca gaccttatgg gcattgaacg
    26641 accagctttc cgcatttcag aaaaagctca atctgctcta cctgagcatc cgcacctaca
    26701 cgatgaccga tgtatggaac aatgccaccg cagggatgct ggaccggaac aacggagaaa
    26761 tcgcacagat agcgatgcga agatggcgca gggcggctac aatcagataa aaacagagag
    26821 aatacaatga gacggacaat cttcataaca atcctcctga tgtgtatggg cataaaagtc
    26881 cacgcacagt tagaccctac ccttaccgga atggttctca tatacaccga aaaggcaaag
    26941 aagaccttga agaaccagga gaaagtcatg ctgctccaaa ccaccggcca catctggaca
    27001 aaggaagagg tggaagccgt caccgacttg cagagggagt tcaacgacta tctggattcg
    27061 ttccgttcgg ttgtcagcta tgccgcccag atatacggct tctaccacga gataacgcac
    27121 ttgacggaga atatgggcga gttcaccggg caactcaaaa actctccgac aaacgccttg
    27181 gcggttgcat tgtccacaaa ccggaataaa atataccgag aattgatata caattccata
    27241 gagataatca acgatatacg gacggtttgc ctgtcggaca acaagatgac ggagaaagaa
    27301 cggatggaaa tcgtgttcgg catccgcccc aagctgcaac tgatgaacaa gaaactgcaa
    27361 cggctgtcga ttgcggtcaa gtacacctcc atgggcgata tatgggcaga aatcagcgaa
    27421 gaggcacgcc cgaaagccaa caaggtggaa atcgcccgtt ctgccataag acggtggaaa
    27481 cggagcggca aagccggagc ttattagaat aacgaataaa acataagaat atgggatttt
    27541 ggagttcatt attaaaaacc ggcggtaaag ccatatcata cggtggcaag gcaactgtcg
    27601 ctaccgggaa aagtgtaggc aacgctgtgc tgcacccctc acagacactg cgtggagcag
    27661 gaagtgcgtt gaagacagcc accgtcggag gtgctgtcgg ttatgtcggt tgggagaaac
    27721 tgaccacgga caagagcgtg gtgcgtatcg tgagcgatgc cgtcataggc gaggaagcca
    27781 ccaatgccat cagcggtaca gccaagtcag ccggggacaa agtaagccag cttaccgaca
    27841 aggcagagca gaccatggcc aatgtaaacc aggcgacaag caacctctca tccactctca
    27901 acggagcatc caacttcttg ggtaatgttt ccaatgggaa cggcggcaat atgttgggta
    27961 acttcttcag caaccttgca aacggcaagg tatcgggaat gagcattgcc ggactgattg
    28021 ccggagcgtt tttggtattc ggccgtttcg gatggctcgg caagattgcc ggtgccttcc
    28081 tgatgatgat gaccatcggc aatcacgcca acctgctgca agaaaccaga gtcgccaacg
    28141 caccccgaca gacaccctcc caacaggaag aacagaccca tagcggagga atgagaagat
    28201 aacatccacg aaaacaatat tgcaacaaca atgaaatata cggaagaaat gattttacat
    28261 tccccaagcg gctactgtat gcccttcgag gaagataaga acaaagaagt aaccttgtca
    28321 aagggctatg gcgaacagaa agatgccgta accggagaaa ctttctttaa tcacggcata
    28381 gactttaatg ccagccaccg ccctttggcg gcagtagcaa gcggtgtcgt atcgagcatc
    28441 ggtacggaca aggaacgtgg cgtgtatatt gtcatacgct acggtaaata cgaagtgacc
    28501 tatgcacatc ttgccaatat attcatacgt ttcggtcaaa aggtaaaggc cggtcagacg
    28561 gtagccatca gtggaagcga ccttcacatg gaagtggcgt ttgacggaga agaactgaat
    28621 ccgatagagt tcctgaccat gctctacggc aatatccagg cattacgcaa agccgggcac
    28681 gaagccaccc acgaatttat ccaatttgac ggtgagatga agacaagata cgaccgtgac
    28741 aaggaagaaa tcgaggaact gatgatccgt ttcctgccgg tctatatgga agacctgttc
    28801 cgtggagcgt acaccgtacc cgaatacacc gagcaatcgc tgcggaacgt gtttgcagtg
    28861 ggtgccacca aaaattattt ctttgagact atgccgagca tggcaaatcc tcttggtatc
    28921 ggtagccgtg ccctgccgtt ggcaagcaag gtacagaatc tcctgatagc cgatttcctg
    28981 aattacctcg cactcaggca caatgtctat ctctccacca tggatgaaga attaaaaaaa
    29041 aacttcaatc cgaggccata gcgagcagtg gaatcattga ccctttggaa gaattggaga
    29101 ttgatgtgca gagttttgac attccgagag tcgtgtccgt ctatcccgac cgtgccggag
    29161 tccgatggtg gacaaaggcc tggttcaaca accgggagga aggcgaactt tcagtagaga
    29221 tagaccggaa caatgccatc cgtttcattc aggacaggat agaaaaggat gaatggttag
    29281 aggagttctt tcccaaacag atggagatat accataacgc catagagcag accaaagaac
    29341 agcttttgaa ccaattgaat tattaggata tgggcgaaat cagacataca gagcgttttg
    29401 cagagataga aaggatattc ggtgactact tcaattgcca ccttgcccct gtcatgcgtg
    29461 acacgcaaag ctatctcaac cgcaagcagg tggaagaaat ggcggaatac tctaccagtg
    29521 cagccggtat tctcagttcg atggcagccg ccaacaatcc gatgatcgat ccgtaccaga
    29581 cactgaaaat aacaggcgaa tggaactcca agactacgga agactatttg gcgatggtca
    29641 aggacaaaat ttccggcaac aaggatatgc agaccgacct ggcgatgatg gccggcgaat
    29701 ggcgttccgc tgtaatcgga gaaatcggca gggatcgtta tgacacatta tccggacaat
    29761 taggatgcga ccttgcctac gcctacacgg attaccgcat ggaacaactg atgatagaca
    29821 ggttggtcaa ggaacgtatg cccaagtcat cggcagacta catcatccgc aaagccgcac
    29881 agaatacgct gttaggcttg tcagcgacac tgggccaatc accgcttgcc gccgagatcg
    29941 agcaacgtgg cgaagcggcc tacaagcctt ccaaattaga aaaagggaca agccgtgtca
    30001 tcggtgcagg gattgacgcc ctctctttag gcggtgtggg cagttggacg agcttcgcaa
    30061 agtttgtcgg agtggatctg gctttttccg cattgacacc ttcccaagac aataaaggaa
    30121 acgggcaatc ggtggaggat tgtatcagta aaggcgtgtt tggaagcgaa gccaacgtct
    30181 ttgcggattt ccgcaaacaa gccaggagca tacagaagga cgagaatccg tacatcatcg
    30241 ctaccaacga gcgactggcc aagaagattc ccattgaaac ttttgatttt acgaaatgga
    30301 tgagtgagca tacgataaca agcaaaccca tgtggagttt ggctgacgaa aagaaaaacg
    30361 aggaacggta taagaatgta cctttggtga ttatgccgga ataccgggaa gcctatctaa
    30421 atagtttaga acagaagcaa aataagggaa gtgaagaatc tgcatctacc gaaatagcaa
    30481 aacaggaagt tcaaaacact accgagcaag ctgttactca acaaatagag gaaagtgaaa
    30541 tgtcgggaca atcgcattcg gaggcaatta cgcaagaaga acaaggacaa caaacggtac
    30601 aatccgacac ccgtaccaac gaaaacggct ggaacggact gttctcctcc ttgggattga
    30661 acggcatggg tgatataggc agaaacttaa gctatgtact ctccatgctg cccgacatcc
    30721 tggtagggat gtttacaggc aggacgaaat ctttggatat aggagacaac atcgtaccta
    30781 tggcaagcgt cttagccggc ttgtttgtca agaaccccgt cctaaaaatg ctgatgattg
    30841 gattgggcgg tgccaacctg ttgaacaagg caggacatga agccattgac tggaaacgga
    30901 atgagggtaa cagcaggcct gtatcggaaa acagacctgt acaataccga agctatccgg
    30961 atgaaccgct caatccgcgc atctccaatc cgatattgaa aggcaacaac ctgatagcga
    31021 caatagaccg tgtaccctgt accatccagc tacccgaaaa ggtaacagcc gcgtaccacg
    31081 caggagcctt gcctctcaac acccttgcca acgccattct cgccaagagc gaccagacca
    31141 gggcgatgat ggagcgcaac tttgagaatg gagaacggga gaccattata agaaccagag
    31201 gaatacaata aaagacaaac atatatgaaa gagaaatcac agtttgagaa aaacgctgcg
    31261 gacaagcagg tcgaattgct ttccgaagca ttgaacggtg cggtcagtgc cggcggtcat
    31321 tggctcaacg ccacgggcaa gggatatccg aagttttatc cgaaaggagt tgcggtcagt
    31381 ccgttcaacg ccctgttcat ggcattgcat tcagacaaga ccggaagcaa gaccaacctc
    31441 ttcaccttat acagcgaagc caaggcacgg ggagagtctg tccgtgaaca cgagaaaggt
    31501 gtgcccttcc tgttctacaa ttggaacaag tatgtaaacc ggaacaatcc caacgacatc
    31561 atttcaagaa acgactacca acaactgcct cctgaagaga ggaaccggta caagggcata
    31621 cacaacaggg agattcgcac actgttcacc ataaaccaga ccctgctgcc ccttgttgac
    31681 aagaatgtat atgagaccct tgtgcagaag cacggcaaca gcgtggagca gggattcggc
    31741 gaaaaggagc tgcgtgaatt gcgcccccgc ttcaatgcct ttgtgcaagc catttccaaa
    31801 aacatggtgc ccgtccgcac ggacggaagc ggcgtggcac attacgacag tcaaaaggat
    31861 gcggtttaca ttccccgtca aaaggatttt gaacattata ccgactatgc acaagagacc
    31921 ttgcggcaaa tcatagcggc taccggacac cagcaacgcc ttgcccgtga aggcatggtg
    31981 atgaaaaacg gagtgccacc caccgaggat gccgtcaagc aggaacatct tatctccgaa
    32041 atcgcttcgg gtatcaagat gctggaaatg ggactgcctg cacgcctgtc ggatgagaat
    32101 atcaagatgg tggactactg gaaccgggaa ctcaaagaga atcccaacct gattgatgcc
    32161 atcgagagcg atgtgaacaa tgccttggag gtcatacgca aggcggaacg aggcgagaaa
    32221 atcgaatatg ccacctttcg taaccaccgg cagaccgagc ggatgcagga gcagatgccc
    32281 aagcatttct ttgtggctga cgagataaag aaacaccccg acaaggataa caagaccatc
    32341 gtgttggtca tagaccgaca gggaaagagt gccgatgtca tcttgcctgc cggcgcatcc
    32401 ctggaggtga acaacgagat aaagggtatg aacaaacagc gcatacagtc tgccctgcag
    32461 aaaagcggca tagagcatgt ccgcttctac aatccagacg gagcattggg gtatcgtccc
    32521 gacgaccgct attttgccga gaagcagatt gaggtggcaa gattgaagag ctggactttg
    32581 gaaacgctct ccaccatcga cgcccatcct gcggtaaagc aagcctatga ccttggcttt
    32641 gagcaaatcc agatggtgca ggacgacaag aaccgttggg cattgtatat aaaacccgaa
    32701 ggcaagtctg gttacagcat ctatcccgac aaggatgacc tgaaccgttt cttcaccact
    32761 ctcaagcagt cgttggataa catcgacaag gtacgtggcg aactggcgaa gaaatactat
    32821 gccttggcgg agagcaaacc cgaactgaag gtggatctgt tccacacatc ggacgagcgc
    32881 atcgacctga accgcatcca gcgtgtggcc gtgttcaaga ccaagaacgg tgcgatactc
    32941 tgcgctccca ccatagacaa tcagaagcca cagccccgaa gtgtcacgcc acaacaatgg
    33001 caacggatgt ggcttgccga agaccggaac agcttcaagc agcatttggc tgcaacactc
    33061 tttgcggatc tgctgaacaa gagccaatca caggagcaga cctcttccga gaaacaggaa
    33121 gaagaggtgg aggcaaaagt cggccggaaa gaggaaatca agcctgaaca gcctaaagag
    33181 gaagtcgagg aacaggaaga ggcatcacga ggtatgcacc gataaacgaa acggaatatg
    33241 agcaagaacc aacaatacgc agaacaatat gccgattttg cgatggagca gatgcgcaag
    33301 tacggcatac ctgcctctgt gaccttggcg caaggcatat tggagagcag caacggacaa
    33361 agccgtctgg ccctgaacga gaacaaccat ttcggcatca aagccactcc aagatggatt
    33421 gcgcagggtg gcaagtacgg attatacacc gatgacaagc ccaacgaaaa gttttgcagt
    33481 tatgacagtg tgggtgattc ttacgaacac cactccagat ttctggtaga gaacaagcgg
    33541 tatgacagat gtttcgcctt ggctcccgat gactataagg gttggacgga agggcttgcc
    33601 aaggcaggat atgccagcgg cagcaattat gcaggaagcc tgcaaaaaat catcgaggtc
    33661 aacggattgc agaagtacga ccggatggtg atggcggaaa cgaaaacgca gagattagag
    33721 attggacaag aaatgaatgt cgatgcaaac aaggaagcgt attcattccc tgtggagcgt
    33781 gcggaattta tgtttgtcac ctctccattc ggtatgcgca ccgacccgat ggatgccggc
    33841 aaacaacaga tgcacaaggg tatcgacatc cgctgcaagg gagatgcggt actggcgact
    33901 gagaacaacg gcaaggtggt ggccgtcaac caaaatgcaa ataccgccgg agggaagtcc
    33961 atcaccatcg aatacccaag gagcgacggg agcaaggtgc agaacaccta tatgcacctg
    34021 tcttctgtcg atgtcaaggt gggcgatacc gtccaagcca ggcagaaagt cggtgtttca
    34081 gggaataccg ggacacggac taccggcgag cacctgcatt tcggggtgac attagtttca
    34141 gcggatgggc agaagcggga tatggacccg gctgtttatc tggctgagat tgccgaaaaa
    34201 ggcaatatca agttacaagc tctgcataac gggaatgacc tgctggcaaa atacaagacg
    34261 gaggcagtgg ccactccaca agaaagcaag gctcaaagtc ctgaagactg gatgaaaaag
    34321 ctgctctctt cggaggacag cggtgtcggc ttatcgggaa ccaacgaccc gattatggat
    34381 atggtggtca aagccttttc ttcgctgatg atgctggcag tagtcattga cagcaaggat
    34441 gaagaggaac agataagtgc cgtttccaaa atggccgatg aacggaaagt ggatttgacc
    34501 ccacttttac cacacatgaa aacctgcgct ctcgtggttg gagagaacaa caaagccgta
    34561 ttgcaggcag acaacggctc catccaattc tcacgggagt tgagtgccaa tgagctgagc
    34621 cgcctgtctg ccacgctgaa cagtccggga ctctcggaag aaagcaagcg tatgcgtgtg
    34681 gccgggatgg tcaatgccct ggtgctgtcg caacaggctt cgcagaactt cgagcaggga
    34741 atgtcggaac aacagagccg ggaggaacaa atcaagagat aatgaaacgc gtgatgatgg
    34801 aaataggatt gttgtttgtc gggttgtcca tggcagcact gcacaccctc gtatgctaca
    34861 aactgttcgg cttggtggcg acactcgtga tcataggggt gcaggtcgtc atcgccatcg
    34921 ggatagtctg gattaaaatc cgtgcgcccg actaagacgg ttgcacaggc aagtggttca
    34981 catttaacag gatattccgg tcaaatggtc ttggtaaaat aaaactacaa gcaatatgat
    35041 aaagtgtaat gtaacaatgt gcggtacaat cagccgtgtg gctaccgtcc gcaccaacag
    35101 cgagaataaa gtgttcacgg cttttgccgt aaattgtgtg gttcctgcca aaagcggtat
    35161 caacaagacc gtggaaatct ccgtcgccaa agatggagag gataacgaac tgaagaattg
    35221 tgccttggga caacgggtaa atgtgtcggg cacgctgact ttcaagaaac gtggggacaa
    35281 cctctatttc aatctgtcag cttcggaggt cactctttcg gcttccaatg aagattccat
    35341 caagggggaa atgcacttcc ggggaaagac tggaaaaatc attgaagaga aaaccgacaa
    35401 gaaaggcaag gaatttttac agttctcagg cttttccgca gagaaagtca atgacggctt
    35461 cgaatacctg tgggtacggt tctttggctt tgactgcaaa cgtgaagagt ggctgcaacc
    35521 ccagacaggc atcgaggtca aaggcgacat ggagctgtcc gtctataacg acaagctgaa
    35581 cctttcctgc aagattgccg agatgtcggc gtatgtcaag cctccgtata cccccaataa
    35641 ctgacaggta tggccggtta caagaaatat aacaacgatg gcccttccgc agaagacaag
    35701 gctcttgacc tttttgcgga aatgatgatc gagcgcatcg agaccatcag caaggactgg
    35761 tcgaagccgt ggattaccga gggaagttta gggtggccga agaacttatc cgggcgtgag
    35821 tacaatggga tgaacgcgct gatgttgctt ctccactgcg agaagaacgg atataagatt
    35881 cctcgtttct gcacctttga ctgtgtgcaa cggttgaaca agccgactga aaagcaggct
    35941 aaagagggtg tggaactgcc gagggttgcc gtcaaccgtg gtgaaaaatc gttcccggtc
    36001 atgcttacca cctttacctg catccataag gagaccaagg agaaaatcaa gtatgacgat
    36061 tacaaaaggc tctccgacga agagaagaag atgtacaacg tatatccgaa gatgcaggtc
    36121 tttcgggtat ttaacgtgtc ccagaccaat cttcaggaag cccgtccgga actttggaac
    36181 aagctggcga atggggatgc cgtaaaattg gacgagagcg agaagatgtc ttttgaaccg
    36241 atggatgtga tgatccgtga taaccgttgg atttgtccca tcaagccgat gcaccaggac
    36301 aaagcctact tctccattac caaaaacgag attgtggttc ctgaaaaggc acagttcaag
    36361 gacggggaat cgtactacgg cacgctttgg catgaaatga cccacagcac aggtatcgag
    36421 gggcagcttg accgcatcaa gcccaccgct ttcggttcgg acgaatatgc ccgtgaggaa
    36481 ttagtggctg aattaggcag cgcattggtg gcacagcgtt acggtatgag caaggctctg
    36541 aaagaagaga gctgcgccta tctgaaatcg tggctggagc aactgaaaga gtctccgcaa
    36601 tttatcaaga ccacgctctt ggatgtgaaa aaggcgactt cgttggttac gcaaaacgtg
    36661 gataagattg cggaagaatt ggaaaagggg aaaaaggaag agcaggacaa caagcaggga
    36721 atgaaagtgg aacaacctgc atccggcgaa aagattttct attcttccgt tgcctacctg
    36781 caatcaaccg atgacacttc ccggctggac gaatttcggg acaagggtga ttatgaagga
    36841 ttgctccaag ctgccaaaga gtattatgac ggaaacggta tcaatgagca atataccttt
    36901 gcctctcctc tccagaacaa gggtgatgac ctgttgattg aagacaagga ttttgccgtg
    36961 gtctataaca acagcgtggg tggcacatac gaggtgatgc tgaaatattc ggaacaggaa
    37021 atccgtgacc atattaccag atatggaacc cgtctggcaa gcgatgacat caaagaggtg
    37081 gcaaaggata tggtggcaga acagttccag gctataacga aacagcgaat acctgtcttt
    37141 gaaatgccga gcggagatat tctctatgcc ggatataaca gggagaccga cacgcttgat
    37201 gtcggtacgg tggtcaatgc cggtcttgtt cccaaccatc agttccccta cgaccacgac
    37261 aattctctcg aatccaacct gcaatccgtc cattcggaat taagtcatat ggaacagtat
    37321 caggaggaag aggtggaata tagcggtggt atgcaccgat aactataatg gggcgtttgc
    37381 gtattcaatc caaccgttgg gagtttgttc tccttgcggt tggattgttg tagctataca
    37441 gtcagtcaat atcatagcct atcacataat cgtcattgat aagcagatag tagtagccgt
    37501 tgccacaatt ccgatattcc ttgctgaagc ctgccgccat atcgccattg tctcgtggct
    37561 cacaccaaag cgttccgtca taaccgcaaa aatcaaagcg gcaagtggaa aatttctctt
    37621 tgtttttcag tgcctcacgg aagagacgca ttgctgtcgt tccacagtat ttttcaatgg
    37681 tggcaagtcc gatacatttg ccgtcgatgc gcgtgcctgt tgtgatgtgg ttctcgtaaa
    37741 ggtttttatc caaatccgca ttggcgattt cacgaagcca ggaatttgta tgtgtggcaa
    37801 gtcgggcaag tttctgatat ttcagaataa gttttctgtc cgtattctcg tcctgttctt
    37861 gcgagggata gtatatttgc tcgatgcttg cccagtctgt aaacacatag ccttttgtcc
    37921 gcttgccgag agcgataatg ccaataatac ctctgtctcg gttgatgaac aagcgtctgc
    37981 gttttcctgc aatccgcacg tataaagacg gaggattggt ttcgttgcga agatagctct
    38041 ccgccggatg aatagttgtc atttccatat ctgtatctat ttttcaatgt ttattttacc
    38101 tgtccttacc agtgtaccat tgcggtaaag catgattcct ccgggcttga accccatttc
    38161 tccgaaggct atgtatttga tataatcccg gtacttctcg cctgaaaagt ttttaccgca
    38221 caagttgttg aaaaacatgg gtattgaaaa cccatcctct cctgacaaca ctgtttttcc
    38281 gttttgtctg atttcgtcta acataccaat ttccaatttt catcatcaat cgtaagtttg
    38341 ttcatttccc aatcatactc cacttccggt gcattggtat agggaaaatt gatggtgtag
    38401 ccgatttgtc cgtaggaaca atgaagcggt gttacacata tttccctgcc aaaataaagg
    38461 ctcggtgtgc agtatttccg ccaattaaag cggtctgcaa ggatattcct gcaaagtctt
    38521 ctgtaatatg atttgttcga taaagtcata attatgtgta ttaaagaaat tccatttcaa
    38581 atgcttgtag caattcgtat atctgttctt cggcatcttc catatccgct acaatatcct
    38641 tgatgtgata aggtgcgcca ttcttgccgt gtccgtcatc gcctatccac aaataggctt
    38701 cataatcggg gtcaaaaccc tcgtaatact cttttacatt ggctatgagg gaatcaatat
    38761 cctcgccctg catatcggca ttgaagagaa aatcctgtcc gtacttggta tagcgttgaa
    38821 actcaaacaa tactcttgtc tcgtttttgt cttcaacgtt cacgctccag cctttttctt
    38881 gggcaatgtc ggttattctc tgtatctgtt cttcggtaat catcttgtga ggggtttgaa
    38941 gtggggaaac tttttacatt tccccacacg tggcttaatc cgttacttga aaataatact
    39001 cctgcttctc gtccgaccat tcagaggtca tttcgtccgc ttctctccat agcttgtcat
    39061 agagaatggc tctttggttg tctctctcgt gccactgcca tatcttgtga ttaagtacaa
    39121 ggcagagttc cgtccaatac ttgtagttgt ctttccattc ggacttggct cgcctgtatg
    39181 tgtcggctat ggctttgtct ccgaatttat cggcaatgga gaagtcctgc caaaaggtgg
    39241 ttatcggctc gtaaccgttc aactctgtta tgttccatct tgggatggcg atttgaagtg
    39301 ctttcataat cgttattgtt tatggttttc gtaatactct tcgggtaaat gatagaggtc
    39361 gttctctgcc aaatggtcta tggcttccgc tgccagacgg cacgcccatt cgttacgctg
    39421 gtcatatctt cccttgtgcc agttctccgc caactgctcc ataaagtaga acatcagctt
    39481 ggctttttcg tttaccaaat agcggtggtc atccgccaat gccttgccca cctttcgggg
    39541 tgaagccatt tttccgttta catagtttga gaactcgtcc gcaaagcgtt tgtcgttctc
    39601 tgataattga ttgttgtact ccatttctaa tgaattttga tgttgaacat tttttctttt
    39661 ccctctggtc ggttctttcc cgtctgaacc gccttggaat tttatggatg cggtaaacgg
    39721 ctgtcggtac tgcttcatgc gacggctttc ccggccaatt actctttcac ggaaaggaag
    39781 aatttgcagg gaatgcactt caagccggag aatcaagcgc gacagcagac ctttgcatct
    39841 gataaaacca agagcggtgt ggacggaaga tgagccacgg cagttatacc tataaaagga
    39901 agatggcttg ggcaaaatat aaaggttgag atacaaaagg aagctgccaa cgcagtttag
    39961 gcaactcata aaaacgggta tcgtgctttt gtgaaaccac gtaagcacga cactcattga
    40021 taagagctaa aaagatgaag gcagtaaatc actcatcctc tgttccggca aaccgcaaaa
    40081 gccccgaatg atagtatctc cgtttacgcc atcaagtttg ctgaacatct gcaaggagtc
    40141 aaatcctctg tcgaattgct ccttgctgac agcaggggca aaatgaataa ctccacgtgg
    40201 atagcttttt gatacaccta tccacccacg caaataatca tcgaaatgtc cgtccgcttt
    40261 tgacacatag aactcttgcg aatgagaact gcaaatgttc tttccgtaca tctcgtctcc
    40321 taaaatcagg attccgattt taggattgta cataaaacgt ctgttggcta tttgaagaat
    40381 atctatgatt tcttgtttga tattgataac catcagggga gtcccaatct ctctttttgt
    40441 acgtttgcct atgccgtaac ctttcttttc catactcctg tcttttatgc tctccgatta
    40501 tgccgatatt tgatattcgt gaatagagca gtatgcgtct tcattttctt ctttccattg
    40561 cttgacaatc gcattgacat cctgacggga tttgacttgt acttcgcaaa tatcctccag
    40621 ccagtcatat acgctttcca agtcttgaaa atactcggaa tagtattctt cctccgaagt
    40681 gcatacatca acatagtatc tgtcaggaaa gtatttgccc tcatcatcat tcgtccaata
    40741 ctcacacata ccaggttctt ccgactgata gaaatatcgc agcgtgggat atttctcaca
    40801 taccatatca aacatttcat tgcagggaga ccacgctgtt tctgtattga atgtaagcac
    40861 ctcaccgttg aatttgaggt tatcccacgt tccacggcaa tatatctttt cccaactttc
    40921 tccgagggct tctacaagac accctaacca cgtagttcca aaaccatttt gaacggacgg
    40981 cttttgtcgt ttttctaaat ctttcattaa ctcatagagt tttttcaatt ctgttgcgtt
    41041 gccctctatc gcatacgcag tgctgcacca attcggcata atcgtaaatt tttattgtta
    41101 gacttgtttt tgtttccctc tgcttggaag ccttccgact tcgccaccgg gatatgtttt
    41161 atgtgcgtta aagactgcgt tacaagggag ataagacaag taaacaaaca gggagttaat
    41221 gaaataccca atttctgcct gccggaaatt gaggaaagtt gtcgttaagt ccggtgattt
    41281 gttagacgaa gcgatacttg gcgttctccc acagcagtac ctttgcacca taaaacaatc
    41341 cgcgcggcgt ggtggtcggc tcaaaggaga atactcaaaa aactataatg ggttgttagt
    41401 aggtaaaaca gatttgacct ttgagtggtg ctataaacgg cacaagtggt tgtataaatg
    41461 gaagaattgc gtacatacta tattttagca ggttacatct accactttgc ggtagaatta
    41521 aaacagtaca tcgctacggc aatcaacgag cagccgtagc cggatacata aaagccacct
    41581 gctacattgt atatcgtaac aagtggctga cgaaaagaac aaaacagatt atgcagtcat
    41641 acgcttacgg atttgccgtg cgtttttctg tacaagttgt atgatgcggt cgtggtattc
    41701 tgtgttttgg ttgcaaagcc ctcttgattg caggactttg aaattttcaa gcgacagttc
    41761 aacagtctca atgcgtttgt catttatccg tgcagagaaa accaaagact tgggtttgag
    41821 gtagtattcg cattgtccca cacaatgatg taaagcattt ccctctttgt aataatcatc
    41881 cacgctctcc aacacgctga ccacaatcag tccgtcagtg aatgacaatc caaagaattt
    41941 gcctttcaac tctctgaact tctcctcatt ctcctgcgcc tttttctgct cggcttcacg
    42001 tttctctcgc tcttggataa cccttagcct ctcttgatag cggtcgtgct cggctttcaa
    42061 atccaatggg cagacataat gggcattgcg aatatccttg ccacaccttt caagcaagcg
    42121 gatgtaatcc acccaaaggg aaatgtcgga tatgtgataa ttgttacgca tcactatctt
    42181 ataagtgttc caatacaaat caaaatacat agggtgttgc agaaaatagt tcaaatccac
    42241 ctttctccct gccttgaaca tcgtttcggc tctactgtcc tgcaacaagg cttcaatcag
    42301 cttcgttggc tcaatctccg acaaagaacc tcttagtccg ttgcgtctta acttggttgt
    42361 ggcagaataa taaggataaa ccttgcagtc ggcaatccgc ctgtaaacat tattgtcgtt
    42421 ccgcagttct atatgattgt caaagacaaa gctgtctata tagtagccga gtgtacgcct
    42481 taatgccgtt acttctgtct tgccattatc atcaatccaa taacggacta cctccattga
    42541 gtaaaaatcg gctttcttgc ccttgcgata atgagccgtc aaaaggaaca ccctctgcac
    42601 ctgtaagccg tcttgggttg tcagtatgga gtaataggat ttgtctttat acttacgctg
    42661 taaggtgttg cgtatctcca actttgtatc gcagcgaggg caacggcagg tcttttctct
    42721 ggtctcgctc cattggtgtc cgcaatccat acaggtggtc ataccgcttt tggtacggaa
    42781 cgcataatga tttaagcaat ggcgaaatgc ccataactct tgcttctcgg tcagtggcag
    42841 taaccgctca ttggcggtta ctaccttgtg ctgtaatctc gtcttggctc tcatagtcct
    42901 aaatcaaata gtgatggttg ggtaacttcc tgtttcgttg ctttctgggt tggcttgggt
    42961 ctgctgttac gttgttgtat cttccgcagt tcctcgtctt gataggcttt gagtgccttt
    43021 tccctcgctt cggcttgctc ttcgggagta agttctaccc tatgattgac tatcacaccg
    43081 cagttgacgg ctttacccac ctctatattg tcttcatcat aatagtgggt acagagagaa
    43141 taaacctcat tgtcggtcat tccacaaccg ccctctttgc atatagccat tgcttggttg
    43201 agtacaaatg tgatgcaatc gtccaaattc ttgttctctt tggcatacga cacggcaaag
    43261 agttcatccg tcttggctct ctcgtccaaa tatgccttta tggtctgctt gaattgttct
    43321 gttcctttca tatcgtctgt tattttgctt gataataaat acttggctca cttgaaacat
    43381 tgtagatgta tgctccgcac cttaccaaca atgcctgttt accataatac aatcgcttca
    43441 ttcctttgac actgcctgtg ttatggaagt tgggaaatcg gctgatatta cagcgttctc
    43501 cctgctcttt ggtcattctt cgtgttctca tagcattaga aatgatagtc aatcactcca
    43561 ccgatataca gcaatgttcc tggctctaat tggcttacca ctcgcagaaa ctcgtaagat
    43621 tgttcggcag ctccgttgat acactgctcg ttcatataga actgataggc ggtatcaagc
    43681 ggatttttga ggtacttttc aagttggtac acctcgccaa tccaaagagt gcagttttct
    43741 gtggtaacgg ctttcgcctt ttcttgcaag gttgccacaa attcctcttt ccatttgtcc
    43801 gcaccgccat tgtaacagat tgtatcatct gaaacaagct caaacatccc tttgggcaga
    43861 atgttgttta tcagattatc aatgtgtgat ttacgctctt cctcgtcaat ctccgagcaa
    43921 tagtcataat agtgtccgtc tccttgttca agggtgcagt cgttcaggat gttgtctttt
    43981 agaggtctct cttctgtgat ttgaaaaatc ttgctgtgca taatctgtct gttttaatcg
    44041 ttgaactttt gttttttctt ttcccttatt ttgaagcctt tatcgctttc ttgtcgggat
    44101 ctgaatttta cgtgcattca gagactgtgg actacagagg gataagacaa gtaaatgcca
    44161 gaggagttga tgaaataccc aatttcagcc tgccggaaat tgtggaaagt tgtcgtcaag
    44221 tccacaaggc attagtggag ccaatcggag aatcggtact tggcgttccc tcgccgcagt
    44281 acctttgcaa aggaaaatgg agccgacaag aatagccgga gaagctcaca gtattgtgat
    44341 aatagaaaca ggtataatgg tctggtagta ggaattataa aacaggataa aatataacaa
    44401 aacggcaaat ttgatgtata tttgccaaaa ttgttataac attcacagca ttatcttact
    44461 acagtataac aaaaatggca aactacattg ttatatgcca aatttgttat aactttgcag
    44521 tataattaaa aaagcaataa tgactgctgt aaatcaaata atagatattg ctaagaaaca
    44581 agatggcata ttcacaagaa aagacttgtt gaatgtggta cgatctggta tgaaaaacat
    44641 atcagaaggg tctcttgttg tgcttttgaa taggatgatt gcagaaaaca aaatcattag
    44701 ggtttcatac gggaaatata aattaaatga agatttgaag catgaatttt tgtatgaacc
    44761 aaacgaattt atgctttcac tcaacaagca tataaaagaa aaatttcctt tcatcgacta
    44821 ttgtatatgg caaccgtctg tatttgcatc aatgatgcta catgtacctg cggtaagaac
    44881 cacattagta gatgttgaaa gggaagcgat ggaatctgtt tttatgtcat tacaaaacgt
    44941 cgaatcagaa atccctattt tcttgaatcc gtcccaagaa gatgtggaca gatacattac
    45001 aaatagagac ttaattattg tccgccctct tgttaaagag gctccattag atgttataaa
    45061 tggctgtccc gttcccacat tggagaaaat gcttgttgat gccatatctg acaaagaact
    45121 acaacatctt caaggcaatg aattatatac tatatattca aacgcattct ctgactatgc
    45181 aatcaaaaaa acaaggttat tgagatatgc ggcacgtcgc aaccgaaaac aaaaagttga
    45241 acaaattatc aatacaatca atatatgata catcccgata gcaggacttt gtcttggata
    45301 gaacaagtcg ccaaagacaa taagataaaa gatataaccc tcgttgagaa aacaatccgt
    45361 gcattctcat tgttggaagc attggctcga tcgggttgcc catttttgtt caagggaggt
    45421 tcgtccttaa tgcttcatct tgatacaggc aaacgacttt ctatcgatat tgatataata
    45481 tgtcctcctg gaacaagaat tgaagattat cttgaaaagt attccgaaga atatggcttt
    45541 ggagaggtca aattggtaga gcgtatctct cgtaccgata ttccgaaaca acacgccaaa
    45601 ttcttttacc aagtggcata tccggcagga ggacgtcagg ataagatttt gttggatgta
    45661 ttattcgagg aaattcatta tgcaaatgta gtggaacttc ctattcaaag ccggttgtta
    45721 aaaacagagg gagaccccat aattgtaaag ttaccaagta aagaggattt gcttggcgat
    45781 aaattaactg catttgctcc tcacacgacc gggattcctt ttttcaaagg agaaaagaat
    45841 tgctcaatgg aaatatgcaa acaactattt gacattgcat ctcttttcga tattgtcaat
    45901 gacttgtcca ttacaacaga gacattcaat aagtttgcga tggttgaact tcaatacaga
    45961 ggtgagagtc ctgatgcaat tcaaaaagtt ttggatgata tttatgatac ggctaaatgt
    46021 atcgtaatgc gtggacaaga taatcaagaa gagtttgaca tgattcaaga tggaataaaa
    46081 aaagtgcgtg gctttattca tagtgaagtc tatactttgg aaagtgctat cacaaatgct
    46141 tcaaaagccg cctatctcgc taaacttatc ggcaaaggaa tacacgaggt aaaacattat
    46201 aatccgtccg aagcgaattt attaatcaac acaacgatac aacctccatt atcaactaag
    46261 ttgaacaaat ttaagaagac aaatgcagaa gcgttcttct attggagtga aatacaaaag
    46321 ttaggataat gcactttata atgcaggaga gccgatccat cacggacagg ctctcccaat
    46381 tcaaatcatg aagcaaaaag tatcttttgt gttctatttc atataaagca atactttggt
    46441 atgtcgattg gcttcggtag gattgtaatc tgagattcct cccttgccga ccttggtaat
    46501 cacatcaact gccagtcctc gcttaaccaa ttctgtggta atataatcgg ctctcgatat
    46561 gcttaacctg tcgtttacat cggcagcccc tgtggctgca tcagccgcac ctacaacagt
    46621 aaccgacaat ccgtatttct ttgcgacacg tgccagttca tcaagattga ccgactgtga
    46681 cttgtctgtc aacttcgctg tgccgagttc aaagaagaaa taaacgggag aaccgatgca
    46741 ctcgttgccg gagtttataa aggacagata atccgagtgg ttataggtgg caatgctgtc
    46801 tccgcccata tttgtcaatt cctgagcttt ggcaagattg tccgattgga cttcatttga
    46861 atttccacta tcatctacat tcacattggc aattccggaa caaaccgcaa ggctgtcatt
    46921 ggacaaaggc gacttgccat cccaacgcct gttcttcaac cttgccctga gcgagttcag
    46981 tccgctgtaa tcatttttag gataaccact gcttaaattt tcatggtctt caaacagccg
    47041 gctgtacttt tcaagcaatc cttcaatatc caaaattttc ttcagttcat tcaaggtacg
    47101 catatccctg tcgtgttgac caatgtacct gcggttgctt tccgacaact ccgaagcata
    47161 ttccactaac cactcgtttt gccgaatata aggagtcgca tcaacggttc gtttccaccc
    47221 gacctttcca atcgtgaaag acagtccggc agaaacggag agcatattgt ccccgaagcg
    47281 gttggcttcc ccatatccgt caaagttttg gaaagtggaa gcattgccaa tctccagcaa
    47341 tgctgccagc cgtttgcaga tgcggtattg cccttgtatg ccataagaaa tggcaaatgg
    47401 tttctgaccg ttctgaggat tatacatcat tccaaggcca acgtatggaa caatgcccca
    47461 acgtggattt tctttctttg catacagtcc gccaaggaca ttatacatca ggtcggcgtg
    47521 gaaatgatga tagtcattgg cagtaaggtt gcagtccttg aaccgccaac ctccataccc
    47581 gattcttgcc ccgatctgtg gagtgaacca tttgccgact gatgcggata acgatggttg
    47641 tacccggtca aacaaatcag cacagccaat cggtctgccg atgaaagcac ttgcaccacc
    47701 ttgcagactg acaaaccagt tgccgttcca tggtgaagtt accagcactc ccttgtgata
    47761 agtcggctct gcggtttgaa acatcagttc cttctccata taggattcat agtgtacact
    47821 atcctgtggg acaggttgta cattcgcctt cgcttgcagt gtgcagcaca ttcctgccat
    47881 tgccattaca aatactattt tatttctcat acgttttctg ttattataga tttcacatcc
    47941 gatgcaggaa actacctcct gccgattatc gtttctgctt tttgccggaa gccggacaca
    48001 tcatcttgct tgccatcatc atacaccgtc ttgcccaggc ccggttgtcc tcgtcatcgt
    48061 cccgtcccca tttaaggtca ctggagcttc caccaccgcc atgcgtttcg gcaaaagtag
    48121 tggcatcatt gaccattccg agaaacagaa gcgtggcgca gtgcatcact tccgatcctt
    48181 gttccgtcag gctttgcagt aatgaaccgt caaatcggga ctgttcggaa agtcccattt
    48241 ctcccaatcg tgccgagtgc tccctgacca atgtttccaa cattacatct ttgagcagca
    48301 catccacgtt ggaatggatt tcacggatgt acttataggc ttcgcctttc aactcctccg
    48361 tgcgctcgcc aatggcatcc atgtcccttt taagggcttc aagttgttgg tcagccgtcc
    48421 gtagcttatc ctgcttgtct tccagtttcg tttggataga agccagttcc ttttccaacc
    48481 gatccctctc tgctataatg gcagccgtat ctccctgatg ggattgaagg gtgcgctcca
    48541 atgccgacaa ctggtattcc ttttccgctt tcgcctttct gaggttgtcc accattgagg
    48601 tcaagccttt taccctgcgt tctgccagac tgatttccac ttttaagtcg gacagtgctt
    48661 ttttatgttg gacaacctgt tcttcgatat tggtacattc ttccgaaagg tgtcggcggt
    48721 actcttcggt ggtgcggtgt tttgcgccgg tctcggatat actgctacca cgtgacattc
    48781 cccaacgctt gttcacttct gagaagtcgc tatgtaactg cttcatccgt gcgctgaact
    48841 cgtacttgtc ctttcctgca aaaatctgct tgtatgcaaa cttgccgtca cggattggca
    48901 acaaggtaca atgcgcatgg ggattcagtt catccatgtg tacgataaaa gctgcaatgt
    48961 tctcctctcc atatttgcca caaacgaatg aatagacgtc cttggcccat tcttcaatat
    49021 ccttgcacct ctcgacatgg gaattgtccg ctcccttatc aaaatttaca atctgactgc
    49081 cgaaagctat ttcgtgcata cgttcacgtg aacctccgaa aataaaattg accaccgtgc
    49141 ggaaacgtgg ctctgccaaa cctgcatttg ggtctttgat tcctctcgcc aacaaattat
    49201 cggacattct ttccgggatg ctccgtgtct tatctatagg gcagacctta cctgccctta
    49261 tctcaaaatt caaatgttct cttgtcggat catagttccc gaccgacacg gcacgctccc
    49321 agcctttttc cgtccacctg cgttggtgtt cgttgctttg tgctgtggtt atgccttttg
    49381 atggtcggaa atccatcacc tgctttgcat ttgccatatt ctcttttctg tttttcggac
    49441 atcctgtttg ccccagcttg ctgcttgtcc gggcacctct cccgatagct ttatgctatt
    49501 ggggtattag gctaccctta cttccctctg tttggtcacc gtgcaagcac tgctgcacgc
    49561 ttggaatggc aagcctataa gcgtagggta tcacggcttg tccgtggtta tacaatctgg
    49621 tagaaactta caacaactgt tgtaaagcct ataaaaggtc cgaatcgtct tcccggcact
    49681 tcgcccatag agccttgaac ggttctttga gcgtggcaag gatctctatc tcatcggaac
    49741 ttgcgcccgt tacctcactt tcaccgtcga ggattatctt ggcggtcatc ttggatgcag
    49801 ccaaaagaga agaccagtca tcccccagct cgaagtggaa gaaatcgata aaagcggtcg
    49861 ggttgtcgaa gcgtgagttt cgtatggttc gcttcagtcc ggcaacggct atgcgctgaa
    49921 ccaatgtccg ggtaagttca tctgctgatt tgtcctttga agacgtttgg cttccagagc
    49981 caccaacagg agaattgtcg gcaatctctt tcaaccgact tacgtagtat tgttcacata
    50041 tatcacccat ctgtgagtct gtcaaattgc cggttatcca tttggataga gcggcatcaa
    50101 actcatcggc aggtcgaatc tccgcttgac cattataagt ggtagtagaa ttgccaatgc
    50161 cggttttgtt gaacaccatt gtaatgacca cactcttggc aaagcgtgta cgatatatat
    50221 gccccatatc ctccaacttg tcgaggaatg tcctgacggt agccctgtgc caatgccatt
    50281 ccgatgcaag gtcggaaatg gtcacatggc attggtcagc ctgcaacacg tattcgtgat
    50341 tcttgaggaa agggactacg aatcctgcca cacacttttc caacaggtcg tggtaggcac
    50401 cggtcttcgt ctttctttct ccgactttct ctttgaggaa gtcgaagagt tccatatccg
    50461 ccaatatctg attggcacat ctattcttgt ctttcgtcat ttcggtcgtt ttttaatttg
    50521 actatctgtg gattcgagtc cactgccact atccacttat tcatggcagc attgcgtgat
    50581 gatgtcaata gtagtattcg gacaatgttc tgggattctt acaccattgt ttcttggttt
    50641 cttcatcttc ccattttgac agcacgttac aagagggata gaacatggca gccaaaagaa
    50701 gaaagccaag cgtaacggct gtggtgtgca gatgcggtac tgcacaaatt gccaatgcga
    50761 gcagtgccgc cagaaagaaa cgcttctgtt cctcgtgaag tccggccaac catttgtccg
    50821 cacgtttgaa cgggaacaga atggcacaca ggatggtaga taccaggatg ccccattctc
    50881 cgaaatgtcc gcctgcatag atatagtgat acaggagaat aagtatgagc atacagcgca
    50941 catagagttt gcgtgcgttc ccgcttgccg tcatccttaa atagaacttt gccataaaaa
    51001 ggcggttgct tctgtacaat gacaagggca atgcaatgag caatgccggg aaaataaatt
    51061 caatgataat ccaaatcata ataaatgggt attgtataat caacgttgtt tgtataatgg
    51121 aaattctgac agttccagtt tgagcaaacc gtatgtggca agctccacaa tggcattgac
    51181 atctttggct tccatataga acgctccctt tttcaggcgg gccttgactt cggaattccg
    51241 cttgcgatac actgccagtg ccctgtccag ttcgtattcg gatatgctcc atttcgtgcc
    51301 gcgctcatag ccattgtgtt tatagattgc ccgaagacga atggtgcgga atgtacatag
    51361 aagtgccccg ctacgcttga agatgatacg tggcgcataa ctgcgactgc catccaaggc
    51421 aatccaactc ttgtgcatat ctataagata aagcatttgc aggtatatcg tccgtctcat
    51481 ataaactccg cgagcaattc ttccttattt ttgcgatatt tgaagtaacc gccctgttcg
    51541 tggagtttca ggtcaagaca gatgtctgaa cacatgaact ctaaggaatc atagatggtc
    51601 acatcaatgc ggtcaacctt gccggtatct gttactcttg cattcatctt gaacatctgc
    51661 ccgaaagtag gattggagta ggcacaagta ctgctgccga agttggtagc ccctgtctga
    51721 atatggtggt aaaggattat cagctccatg tcatcttcca acatatccat tacttcttcc
    51781 agatcgtatt gttccgcaag cggaaaagag agcaggacat aatcgtcata gaactgcggt
    51841 tgctccatcg tggtaacatc caatagttgc ggaagctgga gcggcacgaa ggagagaaag
    51901 tcattgtaaa agagttgtag catatcgttg tagtttttat ttgtttctgt ttttaaatgg
    51961 aagtgatcat ggttttgata aacaattccg gcaaagtttc ccattggtca ttgtcagtac
    52021 cgataatctt gtttagtctg gctatcatag agggcatctg ttctgccaca ccatgcaatg
    52081 acaccctttc cttatcggac agcacagcca tactgaaatc gtccaatgct atgtagggtt
    52141 gcaacagcat ccacacatac gcacgtgcct gtgcatccgt cttgaccttg tcttggttga
    52201 tgtcattcct gcaaaccaac gcattgaaga cgattcttcg gtcggtacgt ttggcgaaat
    52261 atatcagggc atcccggtaa gagaacttgc cgtcctctgc cgactgaaac accgaaaggc
    52321 aacattgctc cgtgtcgcct gtaatggcag aaaggtcggc atctgcaagt tccggaagat
    52381 gggcaaggaa tgaacggaac agcctgtctt cttctcttat aaagtcaagc atctcatctt
    52441 tgctgtttat tccggatttt agagtttggg caagaaaggt cttataaaca gatacgatat
    52501 tctttgtcgt ttttcggtaa agcggtatcg aattcaacga gtcgaagaac ggttctgctg
    52561 tcgcaaccgc ttgtgccagt tccgcatctt gcctgtatgg ggaagtccgt tccttgatga
    52621 gcaatacatc cttgaatgtt tttggggttg aattagccag gcgtatgaac tccgtttgta
    52681 aggaatcgtg caacatttgt atggtggttt catactttgc gtggatatgg tctgccgtgt
    52741 ctttggcgat acggacaaag actgaatccc gaagtgcctg ccaaccgttg acagcctcta
    52801 tcaaatgttc ggtcgaaagg gtttccatac tacgcatatc ggacaaatag gttctgtaag
    52861 cattgacagc actttcactt gtctgaaaca tactgtcatc cgtatttttt ccgcacgatg
    52921 ccaatataaa gatggcaaaa catacggcaa tacctttggc aagaacggat tgctttttta
    52981 agtatgatat aacatcaaca cctttgattt ttctctctga tttcactttg aataagcctt
    53041 tgcacatacg attattattg atttatggct gcaaaggtaa gtataaaagt ttgtataata
    53101 atacttaata atcaaacttt aatatacttt ccttttcata cttacgagta tgatgggcaa
    53161 aacaaacaag agcaaaacaa atgaaaatac agagtataat tattgcaaat tattttcgta
    53221 agtatgattt tattcagatt aaactttgta cctttgcagt tggtatcatt tagcattgaa
    53281 catcagtatg gcaaaagtag gttacatatt caaggcagcc ggttatgacg gcttcgacac
    53341 tgacaaggag tggatggagc aatacggctg tgtacaagtg atagaggaag agaacggaca
    53401 cgagaaactg cgccctcaat ggaaacagct gatggcaagt ctggagcgtg gggacgagtt
    53461 ggttgtctca aaattcagta atgccctgcg tggctcccgt gagctggcga cattcatcga
    53521 gttctgccgg gtaaaggtgg ttcggattgt ctccattcac gacaagattg acagccgtgg
    53581 ggatttgttt ccggaaacaa aagcctccga tgtactggag atgttcggtt ctttgccgga
    53641 ggaatgtgcc atgctacgca aggcttcagc ccacatcatc catctgaaac agaatatcaa
    53701 ccagccgacc aaagagaaaa gtcttagcag ggcggagcgt gaaaagacaa tagtggcgat
    53761 gtacaataac gggcattcca ttgatgacat ctggaaagtg agcggattca acagtcgcag
    53821 ttcggtgttc cgcattctca ataaatatgg tgtttcactc aatcgaggca agttcagcgg
    53881 gccgcttgga aaacgtaaac cgaaagagga ataagttata ttaatgagaa ttatggagta
    53941 tttaaaaatt actgatagct taaaaaagga aattgagccc aagctaaagg attactttct
    54001 tggaagatac tacaagactg aaacaaatca ttatcacgac aatcgtttca tccaattgga
    54061 gaccatcctc cacgatatcg atatacatta cgaatactat caaggatacg ttgaattaca
    54121 cctcgaagga aaattcagcg gttatgaata taatactatt tggagagagt tagttgaaga
    54181 atcttatcag catcaagagc taagttggca tagatgggga aatcgtaata gaggccggtg
    54241 tagaatcaat atttctattc agtctgcaga cgatatcatc aaatgctttg agaaaatatc
    54301 taaaatattc gaccctgtgt tatctccttt atcagcagag catggagacg cagaagtcat
    54361 aatgcagcaa atggaaaatt tagagattct tccacctgcc gatagcatgc aagtagaaaa
    54421 gcttacttac ggcattctaa aaattaaaga attacctttc aataacttca taattccaga
    54481 atatcaacgt ccatataaat ggggtgtaaa gaatgtcaat caattaatca atgatctttt
    54541 gtatttcaag gatagcgaag aatatagact tggtactctt gttcttcatg agaacaatat
    54601 agtggatggt caacaacgta tagtcactct ttctttgatt ttgtacgaac ttttcaacaa
    54661 aaaagaaata aggatcaaga atccttatca agaagtacaa gatagaatta aaacattttg
    54721 gaaacgtact gaatttacaa acgagcattc aattggacat gtgagagaaa accttatagc
    54781 tattgaagat agattggatg atttggatga taatttttta gattttcttt taagcaaatg
    54841 tcagtttgtt gtagttcgcc taccagaaat atctgaagct ttccaatttt ttgattctca
    54901 aaatgcaaga ggaaaagact tggagccaca tgatctattg aaagcttttc atcttagaga
    54961 aatcaaaaga ctctccgaga aagacagtga taacatcaca aaatggcagg atcttgatcc
    55021 acaaagatta gtcgaattgt tcttgacttt atatagagtc aaaagatggg ccaaaaataa
    55081 cagtggaaag gaatttacaa aagacaatat tgacgctttt aagggtatat ctttagaaga
    55141 taatggaaaa atcggagcat gctgttacac atggcatgat taataatatg ttataaa
//
LOCUS       DAOSOM010000011        50548 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_727_length_50548_cov_7.232072, whole genome shotgun sequence.
ACCESSION   DAOSOM010000011 DAOSOM010000000
VERSION     DAOSOM010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 50548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50548
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_727_length_50548_cov_7.232072"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="PLF14_04175"
     CDS             complement(<1..317)
                     /locus_tag="PLF14_04175"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT70529.1"
                     /translation="MTTVSEILQAAQVGETTDWEFKSAKGGLPGSLWETYSAMANTEG
                     GVIVLGVREEGDQARFDGLDSAQIAKYHKLLWDTLHDRGKISLNLLSAKDIAVVNVQG
                     ADLL"
     gene            454..726
                     /locus_tag="PLF14_04180"
     CDS             454..726
                     /locus_tag="PLF14_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70530.1"
                     /translation="MNHEKHRKPSDLLYKEEFSYEQLTDTEKVIGLLRDAPMPVKDLQ
                     AVTKYKSRSRFLDEILNPLIEEEVVYRVGNVKSPTAVIKLKENNKK"
     gene            798..2996
                     /locus_tag="PLF14_04185"
     CDS             798..2996
                     /locus_tag="PLF14_04185"
                     /inference="COORDINATES: protein motif:HMM:NF039200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5703 domain-containing protein"
                     /protein_id="HOT70531.1"
                     /translation="MKNNISHYDYRAQLPDSYFKFIPLGNGRTGASVWVTAQDTTDIH
                     LLLSATDNFSELGRLLKTCKAYISITPSVFDGNSETHLDLKKALLTIKSDKAEMRMYI
                     DANYDVLCFSLKSDIPVSIKYGIDNYRDKPLSSHDMEEDFSNYILSGTARFHNISESA
                     DCVKVSENKKGLTQYHRNDVSFFEYAYFAQNLYSEDKDVYRDPYIGLTFGTYAVSEEL
                     RPVNKGKSSFLETEKPVCDCNIRIWSLTEKADSVDAWEKKIIDLAKSFDCGKSFKCHV
                     TDWEDYWNRYYIYAYGTEDAVLLTRAWIYQKYFNGCAGKGPVPIKFNGSIFVTYPHEV
                     TPGRITWDYRRWGGAFWMQNTRHIYWNMLLSGDFSGMEPLYNLLHQLIPISKHRTFAY
                     YGHNGILLPETFTLCGLYCMSNYGIPQEDGKRYAKGTWLHQPGQIANHYIRWYYTSML
                     ELAYIMLLGARYSDNEKYLNIAYEFSKETLIFYYEHFDTLDGKILLKPISSLETYQDC
                     FNDLPNIAGLDALTKDLLSGDNLPSDIFDICKKIRDILVDYPKDDNKLLPCQILIDKK
                     RRNCENPELYSVFPFYVHTYFNKEHPLFKYAVNAFYQRNEKAGKGWSQDCIQAALLGL
                     TDQAKDILVKQAKMTDERYSFTGIYGPNYDETPDQDHANGLSIGLVHTLVASSKDKNV
                     LFGAWPEDWNVSFRLPLYSSKPVTAVYQNGKVVTLEFEDEQDRIKTEVHI"
     gene            complement(3051..3779)
                     /locus_tag="PLF14_04190"
     CDS             complement(3051..3779)
                     /locus_tag="PLF14_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOT70532.1"
                     /translation="MDNSTVFWVISIFAVSALVVNSIGIYVIYKNKKWAENNKEYFMC
                     FAAGVLITAPLITAFPEALAKNHYAGFAALAGFMFMLFTNKLIKHHTGQEELAFGITA
                     VQGIGIHSLLDGITYTVTFSAGIFTGLASGIGLVVHEFTEGIITFSVLLKGGISAKKA
                     ALYAFLVAGLTTPVGAFVAYPLVQKLSDSVLGLALGFVVGVLIYISAAHLLPEATSHE
                     KKHSMVALCLGIALSLGLSFLHHH"
     gene            complement(3849..6272)
                     /locus_tag="PLF14_04195"
     CDS             complement(3849..6272)
                     /locus_tag="PLF14_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HOT70533.1"
                     /translation="MQDYEKLGVFYIGKKYDIENRKLQDEYVLMKSKNLTTHAAIIGM
                     TGSGKTGLGIGLIEEAVMDNIPVIAIDPKGDLTNLALTFPMLRAEDFRPWINLQDASN
                     KNMDPDSYAQAQADSWRKGLAEWGQDGLRIQRLKNESDVKIYTPGGSSGIGVSVLKSF
                     NAPSKEVLADKDTYRDRISATTTGLLTMLGIAADSFTSKEFILISKILDNSWSEGKSI
                     TLPKLIAEIQHPPFDKIGVMDVNSIYPSKDRFALAMLLNNLIAHPGFEAWLEGEPLDI
                     DRFFYSHSGKPKVSVFSISHLSDNERMFFVTMLLNEVLSWVRSQPGTGSLRAMIYMDE
                     LFGYLPPVANPPSKTPLLTLLKQARAFGLGLVLATQNPVDLDYKALSNAGTWFIGRLQ
                     TERDKLRVLEGLEGAALGGSFDKGRTEQILAGLGQRVFYMHSVYDNEPVIFNTRWAMS
                     YLAGPLTKDQIAKLPEQYITSDRLTVESHISQPDSGAFVAKRDLSTKPVLPPAIKQVF
                     LPEKTCARGKLIYKPAVLGVADAVYNSEKHMITATKSYTILTRFNEGPVTLDWNQSRI
                     IDTELGDIETTPIDGALFSDYPAAAADAKNYDTWKKLLSQYIRTNLTLKLYSSPKLKL
                     VSQYDEDERDFLIRVQHTAHEKRDAALEAMKKKYSSKMQTLDNSIIRARQAVEKQNVM
                     ASQKKMDAMVSTGSAILGALLGRKTISATSVSRLGTAVSRTSKAMKSGQSIDQAEERL
                     KALEAQQETLQTELEQQLKDISYEYESVKDVIETVNIRATSTNITVHLLGLAWVPYEK
                     GELADTILI"
     gene            complement(6406..7824)
                     /locus_tag="PLF14_04200"
     CDS             complement(6406..7824)
                     /locus_tag="PLF14_04200"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT70534.1"
                     /translation="MSKAETEEKRIKRKAKELKELEKWKKEQARPKRKFYLLYLMVVL
                     CLVFIVDTVATEISVKMKAEICHYLFDDSTAVMDLLGMLASAVMIAGAIYKSMSDRFG
                     RKIFLVINTVAMGLGMFIVYLAGEIGSAAVYVIGSLFITFFIAHDMQAVYVIETVPSK
                     YRFTILSIVTSVASLGSILIPVLRRNIMGNNTELWNKVFLIPAILGAVVGLIAMITAR
                     ETDVFLNQRIKFLEMTDEQREAEKEQRHAEAQAQGGLGAAFRFAFRHKQLRWIFICTM
                     VVSMAMIGTMYYQQIMTYGYEAAYGTSTPEVVTDALMVMPIIGAIFTLVRGFISDIFG
                     RKTVAVVMASLAVVGMIGLYLGVTFVWNPVLVGLFTGIYVSGYWCAADAMGGNMSAES
                     SPTNLRASIIGAQSLCNMVGMGVSFPAMIAVAAIAGNNALGIACLFLVVIPLVVAITL
                     FLAKVAETKGIDLNTVRGDEWD"
     gene            complement(7839..10172)
                     /locus_tag="PLF14_04205"
     CDS             complement(7839..10172)
                     /locus_tag="PLF14_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016205610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOT70535.1"
                     /translation="MDIENIISKMTLEDKIVFCEGASFWETRKFEKYGIPAMFVCDGP
                     HGLRKQDMDGKSDMLGVNQSRPATCFPTAVTTANSWDTDLLQAVGSAIGKEARNQNVG
                     VVLGPGCNLKRNPLCGRNFEYISEDPYLAGKLAAAFIRGVEKQGVGTSLKHFAANSQE
                     LSRLTTDSIMDDRTLRELYLTAFEIAVKEGKPATVMCAYNKLNGTYCSDHKQLLTDIL
                     RTEWGFQGLVVTDWGAMNNRIECFKAGCDLNMPGGSNYMAKDTKKAVEAGMLKESDID
                     VSVRRILKQVFRATETLKTPYECDYDAHHELASKAAEQGAVLLKNDDNVLPLKNGDKI
                     AVIGAMAKSMRYQGAGSSHVNAKKLTQPIDALPEAVYASGCDDFGNTTDALIEQAVKV
                     AKEAQTAVIFAGLPERYESEGFDRENMRMPEGHIRMIEAVAEVNTNTVVVLLCGSAVE
                     CPWADKVKGILYVGLCGQAGGKAIANLLYGKVNPSGKLAESWPYSYEDVSSKDIFGKM
                     IDALYTEGIYTGYRYYDKAGIPVRWPFGYGLSYTSFDYTNLNIKEKTVTVTVKNTGDR
                     AGAEVVQLYIGAPQDGIHRPVRELKGFKKIFLQPGENTTVSFELNDRSYSIWQNGWKI
                     PGGTYTVHIADLSESINIAGESIDIPAWQKESWYQSCKGKPTLKDWELLTGKTYTAPV
                     VKKGRFTMNNTVMEMKDYSFVMKIMVKVVERIVAKVNGCKVDYDNPDFRMMINSSLGA
                     PLRSMQIAGGVKDGIMQGLLDMANGHFFRGIGKMIKG"
     gene            complement(10174..12741)
                     /locus_tag="PLF14_04210"
     CDS             complement(10174..12741)
                     /locus_tag="PLF14_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013424583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOT70536.1"
                     /translation="MKAIRLRTEYLQEPIGIDIVKPRFFWNCEGGIKQTAYRIICKRD
                     GETVWDSGKVLSDAMTHITYDGKPLGSRDIIDWSVKLWDEEGKEGEISSSKFEIGLLQ
                     PSDWKAQWITGNYIVCRKKRYPADCFRKIFKCKDIAKARLYITACGVYEALINGRRCG
                     TFVMAPGYTDYRKRIQYQTIDVTELLVSGENTLTIWLADGWYRGSCGSWGLKNQYGSV
                     TKLLVQLEITYTDNTVHTVISDDSWDWSNDGPIRFADNKDGEIYDANYTASYKGKAKL
                     TKNRVIPVASNNVGVSEQEHFTAKKIITPCGKTVLDFGQNMAGYVAFSLNAKARQKIT
                     LRFGEMMNSDGEFTQANFQLVFGKYVTPLQQVIYTCKEGHNQYKTRFSIFGFQYVQVE
                     ADFDINPQDFEAIAVYSNLERTGFFDCSHPLVNGLVHATVWSAKSNHLDVPTDCPTRE
                     RHAWTGDAQIFFETASYLFDFAAFSKKYLFDVYDWQRKNGKLPHIAPDGGADFYMKPM
                     NGSVGWSDVGIIIPYQYYKIFGDRDILQRFYNGMASYARFMISRCGKTMPVFAKRIRL
                     SEGNRKYLVNTGQSYGEWAEPVDVCSFKWQDFATPHPEVSTAYTSYVLGLMAKIAEIS
                     GYEKDAEKFKKYSEGCKNAYRELVSGGKYDLDTDRQAQLVRPLAFNLLNEEQTEFARK
                     RLIKALEHYRWRLGTGFLSTPMILSVLAEYDLEAAYRLLENEEIPGWLSMPLHGATTI
                     WENWEGPYNTQGAGLGSLNHYSKGAVCRWLFDTVCGIRVNGKNQFVVAPRPGGSLTHA
                     SASYNSIYGKVQSKWEKTDNKYEFTITVPANCEAKISLPDGSEHKQPAGTTTYITEV"
     gene            complement(12764..13879)
                     /locus_tag="PLF14_04215"
     CDS             complement(12764..13879)
                     /locus_tag="PLF14_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT70537.1"
                     /translation="MKISKKDAYTWFKFFATLPEEEQLTAHQSEIVYAAFAQIEEVVD
                     KRNRELMESIGGLKTLQNRTYYVGNDDKFPNGCKSCLLGTGLSAVRKTNKCNLECKFC
                     YNYGELDDIPPVGEGMWEIGETKYYEKDIDLLLSVHKKPTGISYVYLEPFMEIEKYYP
                     VIKKFHDANIHQHMYTNGTLVTGQSVKILAECGLDELRFNLGAANCSDKVIDNMRLAA
                     EHIRSVGIETPMTPEFIKGFFEKKNMILNTGIKFINCAELHLNPNNIANYYGENMYIS
                     RHGYISPVWSRELSLKLMKTADDEKWDIVVHDCSNYTKFARGLNLSAKEGKWFGANDY
                     ACEFPAIPYEYFLPVLQDESFLFLSEEPLPAEYADGL"
     gene            complement(13911..15185)
                     /locus_tag="PLF14_04220"
     CDS             complement(13911..15185)
                     /locus_tag="PLF14_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006805238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT70538.1"
                     /translation="MEPKESINVEFKQEVSKTFLKTVSAYANYNDGRIIFGIDDNGII
                     TGLDDAEAQLLRIENMINDSVSPIPNFSIQLKSENNKETIVLTVRKGKDTPYFYDNKA
                     YRRADTATVEVDGFELRRLVMQGMNINYESIKAYSQDLSFNTLEEQLKKVLGIEKLSL
                     DILKTLNLYNIDGYYNIAAELLADTNAIKFSGVDIVKFGKDINTFLYRETITEQSLLL
                     QYKRALELYEQYYTYEEIEGFTRVKKELIPKEAFREALANALVHRVWDINSFIQIAMY
                     DDRIEISSPGGLPPGLSENEYLLDRISVLRNPIIADVFNKLDIIEKFGTGITRIKEQY
                     TDSITKPDFCITENRIIIELPVTEKNILRVSEDEKSVYTLLKNEAQLSRNEIDAALGF
                     DKFKTIRVLNNLIDKNIVDKEGKGSAVVYKLK"
     gene            complement(15358..17592)
                     /locus_tag="PLF14_04225"
     CDS             complement(15358..17592)
                     /locus_tag="PLF14_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOT70539.1"
                     /translation="MRTIYNLNAKWAFTKQANAVPKELPDNWIWVNLPHTWNGIDGQD
                     GGNDYYRNTCYYAKQIDKTDLPTTDCYYLEIQGANSSADVYLNGKHLAHHDGGYSTWR
                     ADITPHLTRDNLLVVAVDNSHNEKVYPQFADFTFYGGLYRDVNLVCVNKSHFSLDYYG
                     GPGVKITPKIAGKDAEVHIETWVTNHKEGQSIRYTIYDAEDKKVTESETDKTEAMLEI
                     KNVHLWHGRKDPYLYKAEVELIEDGKVIDNISSRFGCRTFEIHPENGFILNGEQYPLR
                     GVCRHQDRLDIGNALLPEHHDEDMQLICETGSTTIRLAHYQHDQYFYDLCDEYGMVVW
                     AEIPYISQHNPAANENTISQMKELVIQNYNHPSIVVWGLSNEITMKGDRDPDLLRNHQ
                     ILNDLCHELDSTRLTTVAAVSPCPVDSPYLKIPDVVSYNHYFGWYGGDVSMNGPWFDD
                     FHKKHPDIPIGCSEYGCEALNWHTSRPDQGDYTEEYQAYYHEELIKQLFSRKYLWATH
                     VWNMFDFGADARNEGGTNGRNHKGLVTIDRKYKKDSFYAYKAYLSDEPFVHICGKRYI
                     NRVEDMTKVTVYSNQPEVELLANGVSLGKKKAKCFFYFDVPNKGRTVLTAVAGKQKDE
                     SVINKVEKFDESYRMREKTAILNWFDITAPEGYFSLNDKIEDILKTTRGTLLFMGLFG
                     KMIPKKGEKVMGGFEMNDSMMKMLGGFTVLRLAGMVGTMGLKLTKEDLLDLNAKLNKI
                     KKKE"
     gene            complement(17589..19034)
                     /locus_tag="PLF14_04230"
     CDS             complement(17589..19034)
                     /locus_tag="PLF14_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HOT70540.1"
                     /translation="MKKKQVQLDEKGFRKFSIRDSIAYAAGDFGCNMSFALKGTMAIF
                     WTQFMQMDSILYAALLVIVQVWDAINDPLIGTIIDADRRQYKRNKFLTYIFVGSVGLI
                     VGGALCFIPWRNAPNIAKILIFVFGYVLWDAFYTIANVPYGSLLSLISSDPADRASLS
                     AWRSVGAMAGNILPMVLLPAIIYDNNNNIVGVRVFIAALLMGVLGFIAFQFMIKNTVI
                     RVQEPVKLHEKQPKFNVFKALANFIRNRPAVGATVAAMGMFLGVQGATTAVTVLFQSY
                     FQNVKILGIVQLFALLPIFLFTPLARKMVVKYGKKELATVGSLCSLVSCILMIILPIT
                     PDLKGLIIYIVCLFISSLGLGVYSTVSWAMMGDAIDYNEWKHGAREEGVTYSLHSFFR
                     KLAQGVGPAVALVIMVALGYVEANEGNQTFDVALRMRYLVAALYTFSALLMYIGLGLI
                     YNLDKKKLAQMNEELEAVRVAAEAEEKEAVN"
     gene            complement(19130..20014)
                     /locus_tag="PLF14_04235"
     CDS             complement(19130..20014)
                     /locus_tag="PLF14_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003489494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HOT70541.1"
                     /translation="MTTKRFKKIYIEITNVCNLNCSFCPATKRKPEFMSIDTFGKILN
                     QIKPYTDYIYLHVKGEPLLHPDLSAILDISYEKGFFVNITTNGTLIPEVKDTLLAKPA
                     IRQINISLHSMENNNTDNTYIKNILTFIKQAVSDTSMIISLRLWNLDDKDSFSFGAGK
                     NSAVLQAIENAFSLPFRIEEASATDRGIKIADRIYVNQDFVFEWPDINAKEINTSGFC
                     YGLRNHVAVLVDGTVVPCCLDQDGIMSLGNILKTDFSEIIGSKRAIDIVNGFNNRQIT
                     EELCKKCRYINRFDIKSH"
     gene            complement(20004..20723)
                     /locus_tag="PLF14_04240"
     CDS             complement(20004..20723)
                     /locus_tag="PLF14_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOT70542.1"
                     /translation="MRINNYISSAGICSRRKADELITQGKVKINGKTAVIGQVVNPGD
                     RVEVNGEYVKPSKNFVYIAFNKPVGVICTTERHVKNNIIDFINHPERIFPIGRLDRDS
                     EGLILLTGDGSIVNEILRDKNNYEKEYIVTVDKEITPDFIQKMSKGVRIFNPVKNTFT
                     RTKPCKVVKLDSYVFKIVLTQGLNRQIRRMCASFDYKVVKLRRIRIMDIGLKNLPPGK
                     WRNLTESEVQSLLATRKSDDN"
     gene            complement(20858..21187)
                     /locus_tag="PLF14_04245"
     CDS             complement(20858..21187)
                     /locus_tag="PLF14_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004011769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOT70543.1"
                     /translation="MKKYRLRFLPLFEKDLNQIIDYISIQLENPVAAYSLVDEVYAAI
                     EKRLSCPESFEVFPSKKEHPLPYYRIYVRNYIIFYVISEDTMEVRRILYGRRDLEKHL
                     YIYRKHM"
     gene            complement(21174..21443)
                     /locus_tag="PLF14_04250"
     CDS             complement(21174..21443)
                     /locus_tag="PLF14_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004137988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOT70544.1"
                     /translation="MIVIRPVSDLRNRFTEIEETVKEGKPVYLTKNGYGTMVVISLEQ
                     YTALTENIETKLDEADFMAAESTERYTAKEVFDRARKRIKEHEKI"
     gene            complement(21585..21986)
                     /locus_tag="PLF14_04255"
     CDS             complement(21585..21986)
                     /locus_tag="PLF14_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HOT70545.1"
                     /translation="MKIDRIFVYGSLMKDYRFYKQYLEGKISNISYGKTHGTLYHLPE
                     GYPALVDGDGTVQGEIMEPVDGLLLADLDKFEGYTGTADNNLYIREKRTVLREDGKQQ
                     ECWVYIYANKKYAEEKGISVSDGNWRRFISR"
     gene            complement(22052..23698)
                     /locus_tag="PLF14_04260"
     CDS             complement(22052..23698)
                     /locus_tag="PLF14_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70546.1"
                     /translation="MKYINRVFQSSGQIDVQSVKNKSLRLAILFMVLLLLLTSCGPTL
                     TPRPSEVVTLAPTTASETPAPSETGTPVVEDPVPSEEPLPQPKTMEIDGVTYEMAPLP
                     EFPEPEELDFAAEKKKYQEDEDYSANYLSTVRLISYKEDKVLGYRTEVTARNANKETL
                     KFYPTEYQFYNFFKEYMGEIVNVIWYFDSNAYDENDKELYCCAYDMYIDEETNQDDNF
                     VEGKLTSYKLNSDYDIRITSGVVKLSERIDDLFLVLYVLGEDGYLYDFYYKDTYISLY
                     AGEPGTEVKIEWYIKADRIFFDFGEDDAKGELIPLRRLYEYEVLKEAPSDDYFYSPDG
                     IPYIPGVVIPDHVDLTKEASAEEINTDMESDFISLVKKVDEKPIPLSKYTSYTQFDTV
                     DKNGNTLTFVCTEFAYTTLQKNQATYVIWRNEQLTNIETGKTDIYKFVVGFESHYEIN
                     DNTFANGVTTRKLYVSEKKQVEGIFYKLSEGDYQYLNFLDNDGFLYSFFIGGQMDVES
                     LRNLAQGTKIKVSFYEKEIYIWEAGQRLLDRVAVDYTVIK"
     gene            complement(23815..24138)
                     /locus_tag="PLF14_04265"
     CDS             complement(23815..24138)
                     /locus_tag="PLF14_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014423736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT70547.1"
                     /translation="MSNYKEYKYKALQNPEVKAEYDALQPEFDIIQTMINARNQQNLT
                     QKELSERTGITQADISRIENGTRNPSLNMMKRLAKGLNMQLKLEFVSTCNENSEPYNN
                     VKDES"
     gene            complement(24131..24485)
                     /locus_tag="PLF14_04270"
                     /pseudo
     CDS             complement(24131..24485)
                     /locus_tag="PLF14_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(24732..26366)
                     /locus_tag="PLF14_04275"
     CDS             complement(24732..26366)
                     /locus_tag="PLF14_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70548.1"
                     /translation="MKHINRVFKLSGKLETRSVRNKGLRLLMLFMVLMLLLTSCTPNV
                     SPRPSEVVTLAPTTPSETPTPSETVVPSEEPLPEEPVPEVKTVVIDGVTYEVAPLPDM
                     PEQTELDFAAEKSKYIESDGRYYVDYVSNVRLVSYTNSNAYEGYIEVTGRTAKKEELK
                     FQFYWDTFNKKVESHMGDIVNILWFFLEGDNYSEDKQVYCYGYDLFVDEFIDDFDTSR
                     LSSYALYTDTTPRTLDGVIKYSEMFDDEFRLISVLGNDGKIYDFYLDDSILYDYKKTV
                     GHEVRMNWIIETLILKSDENSDSQPVLLTVRGVMDIELIGQSAYIEELGKSEEGIPYA
                     PSVVIPDDVDLTIEATAEEINTDMESHFVSFVKIAEKRTASFDYYDDLAVLDAVDKHG
                     NSLTFAYSHFNDENLEKDAIVCVIWQNRELTDKATGKTDIYKYVIGIEDYSVDTDAFE
                     YVVSTRKLYTSDKIVMEGTFYELAMGDYYYLVFVGEDGFMYAPFIWGMMESSDDLFEI
                     KKGTKIRVAFYEKEMYIWEAGSRILDRQAVEFSVID"
     gene            26674..27402
                     /locus_tag="PLF14_04280"
     CDS             26674..27402
                     /locus_tag="PLF14_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HOT70549.1"
                     /translation="MEREHQIVKAVYAAKENSQKVDDLIRDYIPFIRSEASKAVSRFC
                     TEQDDEFSIAMIAFHEAILGYERERGAFLNYASMLIRSRIIDYQRKEARHRGTISLYE
                     ETGEDERTLMDELADEKDYYESSANLEATKQEIEELAATIAQYGVSFSDIADNSPKQG
                     RTLEACAQAISYGAQNKEILDKLTKTKKLPLAELVRGSGVERKTLERHRKYILVMLLL
                     HTNGYEIIRGYLRNKIVRKGGSLK"
     gene            27399..28325
                     /locus_tag="PLF14_04285"
     CDS             27399..28325
                     /locus_tag="PLF14_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70550.1"
                     /translation="MKYMVMECHLSYAVVLSRDGRFLKVANRHYEVGQTVTNVIEMQI
                     PQSVPKEKTVVKERERRRWVYAAVAVAAVFLLVFSFALQTGLSTFASIYLSINPEVRI
                     DVNRYDKVIALEGINTEGKKLVEEYHYNNKELNPVIDELVDRAIEMGYLHEGGRITLV
                     LDSGSDEWIATRGVTLGSTLTEHVKEKLSVTIEISDKNNPEDKIVIPIDDDEESIHND
                     TEDDYDDIDDDTEDNDIDDTDNDGEYESDDDEETDDDDDEYESDDDEETDDDDDKYDS
                     DNDEEIDDDDDEETDDDDDTDDDTYSDDSDDS"
     gene            28500..29231
                     /locus_tag="PLF14_04290"
     CDS             28500..29231
                     /locus_tag="PLF14_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70551.1"
                     /translation="MKKYSVLKTLSIILSLVLISAVALVGCNLRRPDSDEEKVVSVGT
                     LLLRVNPEIAIEYDNNGIVVALQAKNEDAKAILKGKEGLVGKETREVISKLVDAIGEA
                     GYFVEEIEGEARQITIEITKGSKLPYDQFIEEVVAEVRKSVEDNNWKAPLGVKNDSDF
                     GLADYNDKDYHSSDDDDDDTDEDDIDDDDDDDTDEDDIDDDDDDTDEDDIDDDDDEDD
                     TDDDIDDDDEDDTDDDIDDDDDTDD"
     gene            complement(29357..29911)
                     /locus_tag="PLF14_04295"
     CDS             complement(29357..29911)
                     /locus_tag="PLF14_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009221522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HOT70552.1"
                     /translation="MNKSLIVVDCQYDFIDGSLACINAENAVKNIIDFINENPKLNVY
                     YSCDWHSENNKSFKRHGGIWPDHCVQDKRGSEIHKDFYSKIKKRKNRPNPNNIFYKGQ
                     DDEVEEYSAFEANNAKGQVLKDVVNQKILVCGIASEYCCRETALEFHKAGKDVEFLSD
                     KVAYVNKDDHEKNLADLKNSITVL"
     gene            complement(29953..30864)
                     /locus_tag="PLF14_04300"
     CDS             complement(29953..30864)
                     /locus_tag="PLF14_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70553.1"
                     /translation="MSYRKPLFWSVIVTIITCTILTACSLTEQAIKPDYKLALEDLKE
                     NYSAEQATADGYVVIDNSTLLAGEKIWVDFVNNTAQGRQAHVRIYQSYSMQDNSYRVK
                     DLRYDGEKYLLSYYDRTGDTKEEFLFQGEYKYLNRSIYSWSKVNPIPAEYYLLSDSED
                     ATAEGYLNSLISSIYKPYNNCNMIYSRTVDSEYAKRASYGVAFCDIDKDGKEEKCYLG
                     WGQTSGVFTFTLTAYENRELEYYNMYRTQWYHPLRFVQAENGKLQIEGVNQNSPPETH
                     IFDITVRKGVIILKLGNTEIEPIKPEQ"
     gene            31080..31355
                     /locus_tag="PLF14_04305"
     CDS             31080..31355
                     /locus_tag="PLF14_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HOT70554.1"
                     /translation="MATVPTQIRIDADVKKQANSLFNELGLDMSGAVNMFLHQCILRG
                     GLPFNVELPKYNEATLEAIAEAIKISRNPDIKGYDTMEELKAALLSE"
     gene            31355..31633
                     /locus_tag="PLF14_04310"
     CDS             31355..31633
                     /locus_tag="PLF14_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HOT70555.1"
                     /translation="MYKVKFTTAYKKSYKRIIKRGLDVSVLDKVIDELRQGNQLDSKH
                     KDHMLKGEYAGFRECHIKPDWLLIYLIEDDILTLTLVDTGSHSDLFRP"
     gene            complement(31748..33448)
                     /locus_tag="PLF14_04315"
     CDS             complement(31748..33448)
                     /locus_tag="PLF14_04315"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOT70556.1"
                     /translation="MKKVNEITFFILIAAIFISTFVLTDVLDMPNLYLIDIEGVSFID
                     GWTWKGDGYKERYYLPHRFDVEKSEPLVIKNTVPDHFPAGSVIALKANVQSVVIKIDG
                     ETVYDIGNDRSKFMGRDLGNYWAVVKTQSEHKGKEIEISLFSHRAASHGFASEVFIGS
                     ESAFYGYLFAQHGLWNIFPPIIFILGVMIILTFFFLGMYKEKNLSLLYLGIYALLMSN
                     WFLGDSEMLQLFTANTYYATRIAYLMTLVSPIPVSLYIREAVPMKKRFFDDFLIGLII
                     INTIVSLGLEHSGILGLSDTVLVAVGLISVMCVYYMAIFIIESVAYKNEKALRELKSL
                     SVIFIFAVAEIISYFVNGQIVSSLYLRIGVLIYIVLVLIYQVKDYRERRRIRVEKEYY
                     EKMAYTDAMTGAGNRARFMEDMDMITKPEKVIIVLADIDRMKYINDYFGHFSGDKAII
                     DTYNTLNTNFGHIGRIYRIGGDEFVVIVEKININEIYAIIKNLEKEVDLIDKESEYDF
                     SISLGVAEYDVSLDEDIHATFLRVDHSMYENKKKHRTAIPAKMPDNQRMYQDKTHNDH
                     "
     gene            complement(33636..35426)
                     /locus_tag="PLF14_04320"
     CDS             complement(33636..35426)
                     /locus_tag="PLF14_04320"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT70557.1"
                     /translation="MKLITETTKCDFCVVGGGISGVCAAIAAARRGLKVVLMQERPVL
                     GGNASSEIRMWICGCKGLNNRETGILEELQLESLYRNPYKLYPVWDSLIYNMVISEPN
                     ITLMLNCSCCNADMDKDKIKAVYGWQMTSQTWQKVEADYFADCSGDSILAPLTGAEYR
                     MGRESKEEFDEDISLTEPDSKTMGNSCLIQARKLDHEVTFKAPPFATKLTREDVEKRH
                     PNIESSYENFWYLELGGDRDTIKDAETIRDELVALAYGMWDYIKNSGDYDAGCWQLDF
                     IGFLPGKRESRRLMGDHILTQKEITADTFFPDTIAYGGWPLDDHDPGGFWHPGRASSI
                     IPTPKPYKIPLSVVYSRNIENLWFAGRNVSMTHAAMSSSRIMATCALLGQAVGEGCAV
                     AKKYKVTPRLARKFHISEIQQNLMEGDCFLPGFLREISPLCKKAALTSDPHVDNLDNL
                     RSGIDRDHHIYDGPQGVYIGKGGTIEYTLEKPTEISKVRMVFDSDLDRNTLPGDLCER
                     THTMRASINIDAPVMTMPKTLVKSYKLTAQLEDGSKIVLIEDTENIKRLIRINVGKKV
                     KRLTLTLLSDWSDNGAYFDDKIHIFSFDFS"
     gene            complement(35524..36792)
                     /locus_tag="PLF14_04325"
     CDS             complement(35524..36792)
                     /locus_tag="PLF14_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOT70558.1"
                     /translation="MIYAIILFVITYILMLTLGKYRTYIALISAALFIISGMLPLNKI
                     FGSIDFNVLLMIAGTMGLVQLFIDSKMPALLADLVMEKVPNVQMAAVALALFAGIISA
                     FVDNVATVLMVAPIALEICKKLKTNPVPFIIAIAVSSNLQGAATLVGDTTAIMLGSYA
                     NMNFMDFFFYKGKPGIFFAVELGALASALILAWLFRKEKQPIPKNTTRTVVTDYVPTV
                     LLAGMIVLLIAASFIPNKPDITNGLICVGLLIAGIIYTAIKTSDKKLIGRMLKAVDLE
                     TIGLLFGLFLVIGGISHMGVIDAVAALLAKLGGGNVFLLYTVIVWASVLFSAFIDNIP
                     YVATMLPVISGLAANLGIDPTLLYFGLLSGATLGGNCTPIGASANIAGIGILRKAGYE
                     VKTKDFVRIGIPFTLAAVIPAYIYFWLLYS"
     gene            complement(36976..38139)
                     /locus_tag="PLF14_04330"
     CDS             complement(36976..38139)
                     /locus_tag="PLF14_04330"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOT70559.1"
                     /translation="MINYPAIYIANGAAVMLLSLILLSSKRPSITRLFDERLFYGMVI
                     FNIIQCCIETVVFILDGNMAYGSHTLLIVLNAALYINDIIFAFLWVIYADYKLFGDMR
                     RIRRIYPYVGIPAILVLICCIINFVTPVFFTINEYNVYQRTGLYIIPFAVTYFYMAYG
                     VLLIYLNRNKVQKYLFMPAILFMIPIIIGSLLQFFFYGYSLIWLGVAIGLVSLFLNIQ
                     NEAAYIDALSGLFNRHYLDSLFLMCLRREDASGVLAGIMLDIDGFKKVNDEFGHTVGD
                     DAISEVGRLLYTAVGDKGVSCRYGGDEFIILMHAKSEKEIIDMVNTIKAHTREFNESS
                     NKPYKIHFSIGYSTYDNEHESTDDFLRKLDTSMYEDKKRNIAERVIPDRRRTT"
     gene            38491..38742
                     /locus_tag="PLF14_04335"
     CDS             38491..38742
                     /locus_tag="PLF14_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOT70560.1"
                     /translation="MNLAKVSSSGQITVPVEVRRALNLKPGDKVLFIQNERGQFTIGN
                     ASAQAIYKAQAAFEGVAEKIGIKNDDDIQALVNEVRYGK"
     gene            complement(38859..39230)
                     /locus_tag="PLF14_04340"
     CDS             complement(38859..39230)
                     /locus_tag="PLF14_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009247468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70561.1"
                     /translation="MKAGYLDLYKWENVTDFFTEKQRHYSNTYMEINEVYDDAVEVSL
                     FSSKDGMYEIYFSYDRFYGIIYVEAENAESKREEIKIELAREYEKHKQVTNEFIRSFV
                     KKHKVCLPNDIFFDASKLFGL"
     gene            complement(39247..40110)
                     /locus_tag="PLF14_04345"
     CDS             complement(39247..40110)
                     /locus_tag="PLF14_04345"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOT70562.1"
                     /translation="MNSVLDSMMSKYPEATSDAEAKNAVKQALQEITLCGLGRSDFYQ
                     NAAFNGGSALRIFHGLQRFSEDLDFSIDNEKIDTFVLDKYVGCINNELASLGIPATFE
                     VDSKSGFIQRGYIKGNCREILNCFGTEEVFTGRVPANEVLKIKLEADAAALSAASYET
                     KYLLQPYPTPVRLYDKCTLFAGKVSAVLSRSWKSRVKGRDLYDYLFYITNKYPMNLAH
                     LESRLKNNGYLKSYQSLDKDILKQLLCERFTTIDYASAKSDVSPFIRDRKVVDFWSAD
                     LFIAVTKDFDF"
     gene            complement(40107..40697)
                     /locus_tag="PLF14_04350"
     CDS             complement(40107..40697)
                     /locus_tag="PLF14_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70563.1"
                     /translation="MIYTTNMLLEKYSNYSNAYNKIQRDCKNGKLVKLMRGLYEDNPS
                     VSPHLCSGYIYGPSYISFDYVLSQSGLIPEYVPNITCATFRKNKVKQYDTPLGSFIYR
                     DIPPEVFPLATIARIEDGYSYIIATPEKALCDKLYSIPQVSSVSAFEELMFDNLRIDK
                     YELNKLDWEIIKSIAPKYNKRNLDYLLKYHAKETEK"
     gene            complement(40766..41230)
                     /locus_tag="PLF14_04355"
                     /pseudo
     CDS             complement(40766..41230)
                     /locus_tag="PLF14_04355"
                     /inference="COORDINATES: protein motif:HMM:NF036995.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
     gene            complement(41522..42430)
                     /locus_tag="PLF14_04360"
     CDS             complement(41522..42430)
                     /locus_tag="PLF14_04360"
                     /inference="COORDINATES: protein motif:HMM:NF037417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOT70564.1"
                     /translation="MKFELNQYHRNISKDELIKDLRMVAEKLNKSYLSRSVYEKNGKY
                     SATPYIRNFGSWLAACDIAGLKTIRNKEDFLRISDEDLLNDMQLVSKRLNTKSISTKD
                     YSEHGKFKVQTILSRFSTWSEALKKANLEQTDFKIISNKDLFDEIERMWSKKGKQPTT
                     TDVKNGLSEYSLNTYIRRFGGWRAALLAFVEYINNDEDYEKAIQEETTEIDNGKKEQN
                     LLENVKEDITPRRKTSRDINLRLRFKVLQRDNFKCCTCGASPAKDPSVKLHVDHIVPW
                     SKGGETVMNNLQTLCSNCNLGKSDLL"
     gene            complement(42518..42727)
                     /locus_tag="PLF14_04365"
     CDS             complement(42518..42727)
                     /locus_tag="PLF14_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70565.1"
                     /translation="MATYKEIQEYVSINFGYLPKTCWIAHIKEIYGLSPKTANNRIDL
                     NKRKHPCPENKLRDIKKAFQHFGMI"
     gene            complement(42869..43102)
                     /locus_tag="PLF14_04370"
     CDS             complement(42869..43102)
                     /locus_tag="PLF14_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70566.1"
                     /translation="MFKGRSSFLTENSYKNRPQYDYVKNQLIEYIQLADGYRVLVTYI
                     TSAGNNRNSGILHINASRIDELVAHPEYLMTKG"
     gene            complement(43133..44236)
                     /locus_tag="PLF14_04375"
     CDS             complement(43133..44236)
                     /locus_tag="PLF14_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019001325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOT70567.1"
                     /translation="MNNDPFKEYIKETEPGKRDKSYAWYTAIGLQAVDGLKTSEYLVH
                     TAVRNIEGEISFEEANALLQTYYEENPARNVENRTEEADKVSVRIAALLSEKAFSFTP
                     SEYISIHRKLFAGIHPHAGRIRDYNITKKEWVLDGATVLYGSATDLRATLEYDFSEEK
                     KFSYKNLTMDEIIHHLAVFISRLWQIHVFGEGNTRTTAVFFIKYLRTLGFNVTNDIFA
                     ENAWYFRNSLVRANYNDLKNGIHETTEFLELFLRNLLLNENHPLHNRTLHISNAFIEP
                     EKVNIGAQKANIEKEKVNIESSFTAKTASHVYKLLETFGFQTIFGRSDVQKALGLKPT
                     RSTALLGEMTEKGFIEPVTGLGKGKYRFRSHKG"
     gene            complement(44257..44408)
                     /locus_tag="PLF14_04380"
                     /pseudo
     CDS             complement(44257..44408)
                     /locus_tag="PLF14_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004812256.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 domain-containing protein"
     gene            complement(44700..45626)
                     /locus_tag="PLF14_04385"
     CDS             complement(44700..45626)
                     /locus_tag="PLF14_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70568.1"
                     /translation="MNVFKVIDRIQELSPCVAVRYRIKRILNQEISSELFDEFYNSKW
                     VELLKTNQLNDGGYGRFHSQNSKIKQKFPTTERATDSIKMLDIQRGNLLVDKLCDYME
                     KILKNEIEWPDGFERNRWYRAAQPLFVASKLSIFGSNCKEYLEVFNCWHTILREAFAD
                     GEYDKERTNKISKELLGCDIDGSYIGLNSIYLIELFGNMQAEISDELKQNYLKWLHHN
                     GKMIGYTSVILSQGFNNKFSELYKVYFLLSKFSCFKKEFEEELDLLIKRRNKEGFWNF
                     GKDFSCQKLSDDWRSRDRMNIDHTIMALLLFV"
     gene            complement(45674..46219)
                     /locus_tag="PLF14_04390"
     CDS             complement(45674..46219)
                     /locus_tag="PLF14_04390"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOT70569.1"
                     /translation="MLTEILQNCVKPFLEELKMIGADTRYVILAGKDIRPCLGCYACQ
                     DVQDEYGCIQHDDMYPIIEDILWADVIVLACPIYSWYCPTLMKSVLDRHYGLNKFYGS
                     ATGSLWAGKKIALLLTHGYDREYACTPFETGVKNLCVHSSLEYIGMYSVRDTDNKASM
                     QTIEAVEGAKWFARKIAQEVK"
     gene            47173..47409
                     /locus_tag="PLF14_04395"
     CDS             47173..47409
                     /locus_tag="PLF14_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70570.1"
                     /translation="MFLGSIQGIPVTGIETIVQNLPELFRVLNKKSLKLQSLGETIGY
                     FCKQYLVSIAKFAYTFAVNGMTIDAVITKLGIVA"
     gene            47532..49523
                     /locus_tag="PLF14_04400"
     CDS             47532..49523
                     /locus_tag="PLF14_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOT70571.1"
                     /translation="MKYYVDVNAVKDGDGSKERPFKRLNEAAKIARPGDEVLVAPGIY
                     REYVDPVHSGTEDARITYISTTPLGAVITGAEQVKNWQHYKGNVWMCRIPNSLFGDYN
                     PYTTLVYGDWYFATPNKHTGCVFLNDKAMYEATTLEECIKGEVYECSWVPEESIYKWY
                     AEQDPDTDETIIYANFQGLNPNEENVEITVRRRCFMPSKTGVSYITIRGFRIDKAATT
                     WAPPAAFQDGMIGPHWSKGWIIEDCEISNSKCAGISLGKYLDPDNEHYFTNKHVKSPT
                     QMERDAVCRGQYHGWLKENIGSHIIRRCNIHHCEQGGIIGRMGSVFSIIEDNHIHHIN
                     NMQELGGAEISGIKLHAAIDVIIRRNHIHHCTMGIWCDWEAQGTRITQNLLHDNQRPP
                     YAKKLEGGMNSQDIFVEVSHGPTLIDNNILLSDVSLRFATQGVALVHNLICGAFTSVG
                     GGTTWRYTPYHIPHRTEVMGFMTILHGDNRFYNNIFIQKWPSEDYVIHNDHDPQRTYR
                     ENRLVGTHVFDEYPNYDEWISQFDFSKRPDMRALEPVHFGHLPIWSEGNVYLNGAKQW
                     KKDVNYLLVENNDQDLKVELVEKDGQYYLCTNIFDYFKDFNVRMINSEVLGKAFEPEQ
                     YYENPDGTPIRFDTDYFGNHRGVQIIPGPFASPSDEIKL"
     gene            complement(49696..50361)
                     /locus_tag="PLF14_04405"
     CDS             complement(49696..50361)
                     /locus_tag="PLF14_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOT70572.1"
                     /translation="MFYPFGYFRFDPTMIILIPAVILTIYAQGKVQSSFAKYLRIPTK
                     RGYTGVQVARNLLDQHGLQNIPIEVSHGKLSDHYDPTKRVLRLSQEVYQGYSIASVSV
                     AAHEVGHAIQHANGYIPLSLRNKVFPVARIGSSLAWPFMIVGLLIPSLGSLMNIGIIL
                     FGMAVLFQIITLPVEFNASSRALNLLDTSGFLAEDESKGAKKVLNAAALTYIETLTLM
                     SEK"
BASE COUNT        14445 a        10728 c         9576 g        15799 t
ORIGIN      
        1 agcaggtctg ctccctggac attcaccacg gcaatatcct tggcagagag caggttcagg
       61 ctgatctttc cacggtcgtg gagcgtatcc caaagcagct tgtgatactt ggcgatctgc
      121 gccgaatcca ggccatcgaa ccgggcctga tcgccctctt cacgcacacc taggacgatg
      181 acaccacctt ccgtgttggc catggcgctg taagtttccc agagactgcc cggcaatcca
      241 cccttggcgg acttgaactc ccaatccgtg gtttccccca cctgggctgc ctggaggatc
      301 tcagaaacgg tggtcacagc accccctgag taaccacaaa atggaatacc aagaaaggaa
      361 aaaccacgaa atacacgaaa ggcacgaaaa agagcgccat aagcaatttc gtgtgattcg
      421 tgctttctgt ggttaacaaa gagtaatgac aacgtgaacc atgaaaaaca tagaaaacca
      481 agtgaccttc tttacaaaga ggagttttct tatgagcaat tgaccgatac cgagaaagta
      541 atagggttgt taagagatgc tccgatgccg gtaaaggatc ttcaggcagt gactaaatat
      601 aaaagcagga gcaggttctt ggacgagata ttgaatcctt taatagaaga agaagttgtc
      661 tatcgtgtgg gtaatgttaa atcacctact gcggttataa agttgaagga gaataataaa
      721 aaatgaggtg tagaccttgc tttgttgtac ataatgtaac agaagggtat ggtataatac
      781 tctaaaggag cgacaatatg aaaaacaata tttcccatta tgattacaga gcgcaattac
      841 ccgattccta ctttaagttt atacctttgg gaaacggtag aacaggagct tcggtgtggg
      901 ttaccgcaca agataccacc gatatccatc tgttgttgtc ggcaaccgat aacttttccg
      961 aattgggcag attattaaaa acatgcaaag catacatctc tattaccccg tctgtttttg
     1021 atggcaattc tgaaacacat ctggatttga aaaaggcgtt gcttacaatt aaatcggata
     1081 aagcggaaat gaggatgtat atagacgcaa attacgatgt gttgtgtttt tctctgaaat
     1141 cggatattcc ggtaagtatt aaatacggta ttgataatta cagagataag ccgttaagct
     1201 cgcatgacat ggaggaagat ttcagcaatt atatattgag cggtaccgca agattccata
     1261 acatatcgga aagtgccgat tgtgtgaagg tatcggaaaa caaaaaaggt cttactcaat
     1321 accacagaaa tgatgtttcc ttttttgagt atgcatactt tgcccaaaac ctctattccg
     1381 aggataagga tgtatatcgc gacccgtata tcggtcttac attcggaaca tatgcggtat
     1441 cggaggagct gagaccggtt aacaaaggga agtcctcttt cttggagacg gaaaaacccg
     1501 tgtgtgactg taatatccgg atatggtctt taacggaaaa agccgattcg gttgatgctt
     1561 gggaaaaaaa gataattgat ttagcaaaaa gttttgactg cggtaagagt tttaaatgtc
     1621 atgttaccga ttgggaagac tactggaacc gctactacat atatgcatac ggaacggaag
     1681 atgccgtttt gctgacaagg gcatggatat atcagaaata cttcaacggc tgtgccggaa
     1741 aaggaccggt tcctataaag ttcaacggct ctatttttgt cacctatcct cacgaggtaa
     1801 caccgggaag gataacctgg gattacagaa gatggggagg ggctttctgg atgcagaata
     1861 cacggcatat ttactggaat atgctgttaa gcggcgactt ttcgggaatg gagccgttat
     1921 acaatctttt acatcaatta atacccatat ccaaacacag aacatttgcc tattacggac
     1981 ataacggaat actgcttccc gagactttca cattgtgcgg actttactgc atgagcaact
     2041 acggtatccc tcaggaagac ggtaaaaggt atgcaaaagg cacatggctg catcagcccg
     2101 gacagatagc caaccactat attcgctggt actatacaag catgctggaa ttggcatata
     2161 ttatgctgtt aggtgcaaga tattcggaca atgaaaaata tctgaatata gcatatgagt
     2221 tttccaaaga aacacttatt ttctactatg agcattttga cactcttgac ggcaagattc
     2281 tcttaaaacc tatatcatca ttggaaacat accaggattg ctttaacgat ttgcccaata
     2341 tagcgggact ggatgctttg acaaaagatt tgttaagcgg tgataatctt ccttcggata
     2401 tttttgatat atgcaaaaag ataagggata tacttgtaga ctatcccaag gatgacaaca
     2461 aactgttgcc ttgtcagata ttgattgata agaaaagaag aaattgcgaa aatcccgagc
     2521 tttacagtgt atttcctttt tatgtccaca catactttaa taaagagcat ccgcttttca
     2581 agtatgccgt caatgcattt tatcaaagga acgagaaagc ggggaaaggc tggtcgcagg
     2641 actgcattca ggctgcttta ttgggtctta ccgatcaggc aaaagatata ctggtaaagc
     2701 aggcaaaaat gacagatgag agatattcct ttaccggaat atacggtccc aactatgatg
     2761 aaactcccga ccaggatcat gcaaacggat tgagtatagg gttggttcat acgcttgttg
     2821 cctcctcaaa ggacaaaaat gtactgttcg gagcctggcc tgaagactgg aacgtatctt
     2881 tcaggctgcc gctttattcg tcaaaaccgg taacggctgt ttaccaaaac ggtaaagtag
     2941 ttacactgga atttgaagat gagcaggaca gaataaagac ggaagtgcat atatagcctt
     3001 atgtttttta agtaacggat aaataaaggt tatatacgaa attgatagaa tcaatgatga
     3061 tgtaaaaagg acaatcccag tgatagtgca atacccaaac acaatgcgac cattgagtgt
     3121 ttcttttcgt gcgatgtcgc ttcgggtaac agatgagctg ccgatatgta tatcagtaca
     3181 cctactacaa atcctaaagc aagacccaat acggaatcac ttaacttttg caccaaagga
     3241 tatgccacga atgctcctac gggagtggtt aatcccgcaa caagaaaagc ataaagtgcg
     3301 gccttttttg cactgatacc gccttttaac agaacggaaa aggtaatgat tccttcggta
     3361 aactcatgta ccacaagacc gatacccgat gcaagaccgg taaatatgcc tgcactgaaa
     3421 gtgaccgtat atgttattcc gtccagcaac gaatgtatgc ctattccctg aacggcggta
     3481 ataccgaaag ccaattcttc ctgaccggta tgatgtttta ttaatttatt ggtaaaaagc
     3541 ataaacatga aacctgccag agcggcaaat ccggcataat gattctttgc aagcgcttcg
     3601 ggaaaagcgg ttataagagg agcggttatt aagacacctg cagcaaagca catgaaatat
     3661 tctttgttat tctccgccca tttcttgttt ttataaataa cataaatacc gatagagtta
     3721 actaccagcg cacttaccgc aaatatcgat attacccaaa atactgtcga gttatccatg
     3781 actgttcctt cctttatatt acaccttaac cgccgtttct ttaccttgca attaaaacgg
     3841 taaagcggtt atataaggat tgtatcggca agttctccct tctcataagg aacccaggca
     3901 agacctaata aatgtacggt aatattggtg cttgttgccc ggatattgac ggtttctatt
     3961 acatccttta cactttcata ttcgtaagat atgtctttca gttgctgttc cagttcggtc
     4021 tgaagtgttt cctgctgtgc ttccagggct ttcagccttt cttccgcctg gtctatactt
     4081 tgtccgctct tcatggcttt ggatgttctg cttaccgccg tacccaatcg ggaaaccgaa
     4141 gtggcactga tggtttttct tcccagaagg gctcccaata tggcacttcc ggtggacacc
     4201 attgcatcca tctttttctg ggaggccatt acattctgtt tttccaccgc ctgacgtgcc
     4261 cgtataatgg aattatccag agtctgcatt ttggaggagt atttcttttt catggcctcc
     4321 aaagcggcat cgcgcttttc gtgtgcggta tgctgtaccc ttatcaggaa gtctctttca
     4381 tcttcatcat actgagaaac cagtttgagt ttgggagacg aataaagttt caatgttaag
     4441 ttggtgcgta tatactgtga caataatttt ttccacgtat cgtaattctt tgcatcggcc
     4501 gcagcggcgg ggtaatcgga gaaaagcgca ccgtcaatcg gggttgtttc aatatcgccc
     4561 agttccgtat ctataatccg tgactgattc cagtccaaag ttaccggtcc ttcattgaag
     4621 cgggtgagta tggtatagga tttggtggcg gttatcatat gcttttcact gttatacacc
     4681 gcatctgcta ctcctaatac cgccggtttg tagataagct tgccacgggc gcaggttttt
     4741 tcgggaagaa agacctgctt gattgcggga ggtaataccg gttttgtcga taaatcccgt
     4801 tttgctacaa aagcacccga atcgggttgg gaaatatgac tctccacggt taacctgtcc
     4861 gaagttatat actgctcggg cagttttgca atctgatctt ttgtcagcgg tcctgcaaga
     4921 taagacatcg cccatcgggt attgaatatg accggctcat tgtcgtaaac actgtgcatg
     4981 tagaaaactc tttgccccag acccgctaaa atctgttcgg tgcgaccttt atcgaaacta
     5041 cctcccaatg cggcaccttc cagtccttcc agtactctta atttgtctct ctcggtctgc
     5101 aacctcccta taaaccatgt tccggcattg gaaagagctt tgtaatccag atccacgggg
     5161 ttctgtgttg caagtaccag gcctaagccg aaagctcggg cttgtttaag aagcgtaagt
     5221 aaaggtgttt tggaaggagg attggcaacg gggggaagat aaccgaacaa ttcatccata
     5281 tatatcattg cccgtaagct tccggtaccc ggctgacttc tgacccagga cagcacttca
     5341 ttcaacagca tggtgacaaa gaacatcctc tcattgtccg aaagatgaga tatggaaaat
     5401 accgacactt tgggtttgcc cgaatggctg taaaagaacc tgtcgatatc taagggttct
     5461 ccttccagcc aggcttcaaa tccggggtgt gctattaagt tattcaacag catagccaat
     5521 gcaaagcggt cttttgacgg atatatgctg tttacatcca taacgcctat tttatcaaac
     5581 ggcggatgct gaatttccgc aataagcttt ggaagcgtta tgctttttcc ttccgaccag
     5641 ctgttgtcta aaattttgga tatgagtatg aattctttac ttgtaaagga gtcggcagct
     5701 attcccagca tagtcagaag ccccgtagtg gtggcactta ttctgtcacg atatgtatct
     5761 ttatccgcta gaacttcctt agagggagca ttgaaagact tcaataccga tacgcctata
     5821 ccggaagagc ctcccggagt atatattttc acatccgact cgtttttcaa tcgctgtatt
     5881 cttaaaccgt cctgacccca ttcggccaat ccctttctcc aggaatcggc ctgagcctgt
     5941 gcataagaat cggggtccat atttttattg gaagcatcct gaagatttat ccacggacgg
     6001 aaatcttccg ctctcagcat ggggaatgtc agagcaaggt ttgtcagatc gcctttggga
     6061 tcaatggcta taacgggaat attatccatt acggcttctt cgattagtcc tattcccaac
     6121 cccgttttac cgcttccggt cattccgata atggcggcat gcgtggtcag attctttgat
     6181 ttcataagaa catattcatc ctgcaactta cggttttcta tgtcatattt tttccctata
     6241 tagaacactc cgagcttttc ataatcctgc attatatatc cctccgtttt tccatacatc
     6301 atgatatgtt aatagaatcg gtacttaaat tatataccat ttcagccact atatacttat
     6361 taaatgtttt cgggttcact cagtgactcc gattttaata tattgtcagt cccactcgtc
     6421 tccgcgaacg gtattgagat cgataccttt ggtttccgct accttggcta agaacagtgt
     6481 aatggcaacc accagcggaa ttaccaccag gaacaggcag gctataccca aagcattatt
     6541 tcccgcaatc gcagcaaccg caatcatagc ggggaaggaa acacccatgc ctaccatgtt
     6601 acacagactc tgggctccta tgatggaagc acgcaagttg gtgggagaag actcggcgga
     6661 catattgcct cccatagcat cggctgcaca ccagtatcct gaaacatata taccggtaaa
     6721 cagtcctacc agcaccggat tccatacaaa ggtaacacct aaatacaaac cgatcatgcc
     6781 tacaaccgcc aaagaggcca ttacaacggc aacggtcttt ctgccgaata tgtcactgat
     6841 aaaacctctg accaatgtga atatggcacc tattatgggc ataaccatca gcgcatcggt
     6901 aactacctcg ggagtggaag tgccgtaagc agcctcgtaa ccgtaggtca taatctgctg
     6961 gtagtacatg gtacctatca ttgccatgga aaccaccatt gtgcatataa atatccagcg
     7021 taactgttta tggcggaaag caaatctgaa tgccgcccct aaacctccct gagcctgagc
     7081 ctctgcatgg cgttgctctt tttccgcctc acgctgctca tcggtcattt ccaggaactt
     7141 tattctttgg ttaaggaaaa catcggtttc ccgtgcggta atcattgcaa tcagacctac
     7201 aactgcacct aaaatagcgg ggataagaaa tactttattc caaagttcag tattattgcc
     7261 cattatatta cgtcgcaata ccggaataag aatactgccc agactggcaa cggaagtgac
     7321 tatcgataat atggtaaatc ggtacttgga aggtacggtt tctatcacat ataccgcctg
     7381 catatcatgg gcgatgaaga aggtgataaa cagagaacct atgacgtaaa cggcagcgga
     7441 acctatttca cctgccaagt agacaataaa catacccaag cccatggcaa cggtgtttat
     7501 aaccaggaat atctttcggc cgaaccggtc ggacatggac ttgtatattg ctcctgcaat
     7561 cataaccgcc gaagccagca tacctaataa atccatgaca gccgtagagt cgtcaaacag
     7621 gtagtggcat atttccgcct tcattttgac acttatttcg gttgcaacgg tatccactat
     7681 gaacaccaga cacagcacta ccatgagata caaaagatag aatttacgtt ttggacgagc
     7741 ctgttctttt ttccactttt ccaattcttt cagttcttta gcttttcttt taatgcgctt
     7801 ttcctcggtt tccgctttac tcataattat tccccctgtt atccttttat catttttccg
     7861 attccccgga agaagtgacc gtttgccata tccaaaagtc cctgcataat accgtctttt
     7921 acaccgcctg caatctgcat gctgcgaagc ggtgcaccca atgaggagtt aatcatcatt
     7981 cgaaaatccg gattgtcata gtccaccttg caaccgttta cttttgcaac tatccgttcc
     8041 acaaccttga ccattatttt cattacaaac gagtaatcct tcatctccat tacggtatta
     8101 ttcatggtaa accgaccttt tttcactacc ggagcggtat aggtcttacc cgtcaataac
     8161 tcccaatctt tcaatgtagg ctttcctttg cacgactgat accatgattc tttctgccat
     8221 gcgggaatat ctatactttc acctgcaatg ttaatacttt cagacaaatc cgcaatgtgt
     8281 acggtgtatg taccgcccgg gattttccaa ccgttctgcc atattgagta gctgcggtca
     8341 ttcagttcaa aggaaactgt tgtattttca ccgggctgta agaatatctt tttgaaacct
     8401 ttcagttctc taaccggacg atggatgccg tcctgaggtg caccgatata cagttgcaca
     8461 acctccgctc ccgcacggtc gccggtattt ttaacggtaa ccgtaacggt tttttccttt
     8521 atgttcagat ttgtataatc aaaacttgta taactgagac cgtaaccgaa cggccaacgg
     8581 acgggaatac cggctttgtc ataataccga tagccggtat agataccctc tgtatacaga
     8641 gcgtcaatca ttttaccgaa aatatctttc gaagacacat cctcgtaaga atagggccag
     8701 ctttccgcca atttgcctga gggatttact ttaccgtaga gcagattggc tatggcttta
     8761 ccgcccgcct gaccgcaaag acccacatag agaatgccct ttactttgtc cgcccaggga
     8821 cattccaccg cacttccgca gagcagtacc actacggtat ttgtgtttac ttcagcaaca
     8881 gcctcaatca tgcgtatatg accttccggc atcctcatat tctccctgtc aaaaccctcc
     8941 gactcgtagc gttcgggaag acctgcaaag ataacagccg tttgtgcttc ctttgcaacc
     9001 tttaccgctt gctcaatcag tgcatcggtg gtgttaccga agtcatcgca tcccgatgca
     9061 taaaccgctt cgggaagagc atcaataggc tgagttaatt tctttgcatt tacatgactg
     9121 ctgcccgcac cctgatagcg catggatttg gccatggcgc ctatgacggc aattttatcg
     9181 ccgtttttca gcggcaaaac attatcatca tttttaagaa gtaccgcacc ctgttccgcc
     9241 gccttactgg caagctcatg gtgggcatca tagtcgcatt cataaggtgt tttcaaggtt
     9301 tccgtcgccc ggaatacctg cttcagtatt cttcttacgg aaacatcaat atcgctttct
     9361 ttcagcatac cggcttcaac ggcctttttt gtatcctttg ccatatagtt actgccgccc
     9421 ggcatattca gatcgcagcc tgccttaaaa cattcgatac ggttgttcat tgcaccccag
     9481 tcggttacaa ccaggccctg aaaaccccat tcggtacgaa gaatgtcggt cagcaattgc
     9541 ttatggtcgc tgcaataggt gccattcagt ttattgtatg cacacatgac ggtagcgggc
     9601 ttgccctctt tcaccgctat ttcaaaggcg gtaagataca attctctgag agtacggtca
     9661 tccataatcg agtcggtggt aagacggctc aactcctgag aattggctgc aaagtgcttg
     9721 aggctggtac ctacaccttg cttttcaact ccccgtataa atgccgccgc caatttacct
     9781 gcaaggtaag gatcttcgga aatatactcg aagtttctgc cgcacagcgg attgcgtttc
     9841 agattacatc ccggccctaa aaccacgccc acattctgat tgcgtgcttc cttgccgatt
     9901 gcactgccta ctgcctgcaa caggtcggta tcccagctgt tggcagtggt cactgcggtg
     9961 ggaaagcagg tggcaggtct tgattggttg actcccagca tgtccgattt gccgtccata
    10021 tcctgtttgc gcagaccgtg gggtccgtca cagacaaaca tggcgggaat accgtacttt
    10081 tcaaatttcc ttgtttccca gaaagaggca ccttcacaaa agacaatctt gtcttctaat
    10141 gtcattttgg aaataatgtt ttcaatatcc atatcaaacc tccgtaatat atgtagttgt
    10201 acctgccggc tgcttgtgtt cgctgccgtc gggcagagat atctttgcct cacagtttgc
    10261 aggtacggtt attgtaaatt catatttatt gtccgttttt tcccacttgc tttgtacttt
    10321 accgtaaata ctgttatacg aagcggatgc atgagttaaa ctgccgcccg gtcggggagc
    10381 aacaacaaat tgattttttc cgtttactct tatgccgcac acggtatcaa acagccatcg
    10441 gcaaacggca cctttggaat aatggttaag tgaaccgagt ccggcacctt gggtattata
    10501 cgggccttcc cagttctccc agatggtggt ggcaccgtga aggggcatgg agagccagcc
    10561 gggaatttcc tcgttctcca ataacctgta tgccgcctct aagtcgtatt cagccagtac
    10621 gcttaaaatc atgggtgtgg ataagaaacc ggtacccaac cgccaacgat agtgttccag
    10681 tgccttaatc agccgtttcc ttgcaaattc cgtctgttct tcattaagca gattaaatgc
    10741 taatggacgc accaattgcg cctgtcggtc ggtatccaag tcatacttac cgcccgatac
    10801 cagttcccgg tatgcatttt tgcaaccttc gctgtatttc ttaaacttct cggcatcctt
    10861 ttcatagcct gagatttccg caatttttgc cattaaaccc agaacataag acgtgtaggc
    10921 ggtggatact tcgggatgag gtgttgcaaa gtcctgccac ttaaacgagc agacatctac
    10981 cggttccgcc cattcgccgt aactttgacc cgtattgaca agatatttgc gattgccttc
    11041 agacaacctg attctcttgg caaatacagg catggtttta ccgcaacgac taatcataaa
    11101 gcgggcatag cttgccatac cgttatagaa tctttgaaga atatccctgt caccgaatat
    11161 tttgtaatac tgatagggaa ttataattcc cacatccgac cagcctaccg aaccgttcat
    11221 aggtttcata tagaagtcgg cacctccgtc gggggcaata tggggcagtt taccgttctt
    11281 tctttgccag tcgtaaacat caaataaata ttttttagag aatgcggcaa aatcaaaaag
    11341 ataacttgcc gtctcaaaga aaatctgagc atcacccgtc catgcgtggc gttccctggt
    11401 agggcagtct gtgggaacat ccaggtgatt gctttttgcc gaccacacag tggcatgtac
    11461 cagaccgttt accaaaggat gagagcagtc aaagaaacct gtccgctcca ggtttgagta
    11521 aacggcgatt gcttcaaagt cttggggatt tatgtcaaaa tccgcctcta cctgtacata
    11581 ctgaaacccg aatatcgaaa agcgtgtctt gtattgattg tgtccttcct tgcaggtata
    11641 tataacctgc tgcaggggag taacatattt tccgaaaaca agctggaagt tggcttgagt
    11701 gaattcgccg tcggagttca tcatttctcc gaagcggaga gttattttct gtcgagcttt
    11761 agcgtttaaa ctgaaagcaa cataacccgc catattctgt ccgaagtcca acacggtttt
    11821 accgcaaggg gttataatct ttttggcggt aaaatgttcc tgctcggaaa ctcccacgtt
    11881 attggaagca accggaataa ccctgttctt tgtcaatttc gctttccctt tatatgaagc
    11941 cgtatagttt gcatcgtaga tttctccgtc cttattgtct gcaaatcgaa tgggaccgtc
    12001 attgctccaa tcccagctgt catcggaaat gacggtgtga acggtattgt cggtataggt
    12061 tatttccagc tgaactaaaa gcttggttac gctgccgtat tgatttttca agccccagga
    12121 accgcaggag ccgcgatacc agccgtcagc aagccatatg gtcagtgtat tttctcccga
    12181 aaccagcaac tcggtcacat caattgtctg atattgaatt ctttttcggt agtcggtata
    12241 gccgggagcc attacaaatg taccgcacct tcggccatta atcagtgctt cgtaaactcc
    12301 gcaggcggtg atatacaacc gtgcttttgc aatatcctta cacttaaaaa ttttgcggaa
    12361 acaatctgcg ggatagcgtt tttttctgca tacaatatag tttcctgtaa tccactgtgc
    12421 cttccaatcg gaaggttgta acagccctat ttcaaactta gaagaagata tttcaccttc
    12481 ttttccttcc tcatcccaga gtttaaccga ccagtcgata atatcacgac tgccgagagg
    12541 cttgccgtca taagtaatat gagtcatggc atcggacaga acttttccgc tgtcccagac
    12601 ggtttctccg tctcgcttgc agataatccg gtaagcggtc tgttttatcc cgccctcgca
    12661 gttccagaaa aaacgaggct tgactatatc aattcctatc ggttcctgca gatattccgt
    12721 tctcaatcta atggctttca taatcgtcac cttaatagtt atattataac ccgtcggcat
    12781 attctgccgg aagcggttct tcacttaaga ataggaaact ctcatcctgt aataccggaa
    12841 ggaagtattc atagggaatg gcaggaaatt cacatgcata gtcatttgct ccgaaccatt
    12901 ttccttcttt ggccgacaga tttagtcctc ttgcaaattt ggtatagttg gagcagtcat
    12961 gtactacaat atcccatttc tcatcgtcgg ctgtcttcat cagcttcaaa gaaagttcac
    13021 ggctccagac aggggagata tagccgtgtc gggaaatata catattctca ccgtagtagt
    13081 tagcaatatt gttggggttt aagtgaagct cggcacagtt tatgaacttt atgccggtat
    13141 ttaaaatcat attcttcttt tcaaaaaaac cttttataaa ttccggtgtc ataggagtct
    13201 caataccgac actccgtata tgctctgccg ctaatctcat gttatcaata actttatccg
    13261 aacaatttgc ggcacctaaa ttgaacctga gttcgtcaag accgcattcg gctaatatct
    13321 taaccgattg tccggtgacc aatgttccgt ttgtgtacat atgctggtga atgtttgcat
    13381 cgtgaaactt ctttataacc ggataatact tttcaatttc catgaaaggc tctaaatata
    13441 cataagatat gccggtaggc tttttgtgta cggaaagcaa taaatcgatg tctttttcat
    13501 aatattttgt ttctcctatt tcccacatgc cttcacctac gggaggtatg tcgtccagtt
    13561 caccgtagtt atagcagaac ttgcattcaa ggttacactt attggttttt cttacggcac
    13621 ttaagcccgt tcccagaaga caggacttac agccgtttgg gaacttgtcg tcatttccca
    13681 cataatatgt cctgttttgc agagttttaa ggccgcctat gctttccatc aactctcggt
    13741 ttcttttatc cacaacctct tcaatctgag caaatgcggc atacactatt tccgactgat
    13801 gcgccgtgag ctgttcctct tcaggtagtg ttgcaaagaa tttaaaccat gtgtaagcat
    13861 cttttttgga tattttcata atttgtctcc ctgcgatact tttagcggcg ttacttcaat
    13921 ttatatacaa ccgccgatcc ttttccctct ttatcaacaa tattcttgtc gataaggtta
    13981 ttcaatactc tgattgtttt aaacttatca aaacccagtg ccgcatcaat ttcatttctg
    14041 gataattgtg cctcattttt caatagtgta tataccgatt tttcgtcttc cgatactctt
    14101 aaaatgttct tttccgtaac aggcaattct attattatcc tgttttcggt tatgcaaaaa
    14161 tccggttttg ttatactgtc ggtatattgt tctttaattc ttgttatgcc tgttccgaat
    14221 ttttcaatta tgtcgagttt attaaacaca tccgctatta tgggatttct taaaacggat
    14281 attctgtcca gtaaatactc attttccgac agtccggggg gtaagcctcc cggtgaactt
    14341 atctcgattc tgtcatcata catggcaatt tggataaagg aattaatgtc ccataccctg
    14401 tgtaccaagg catttgccag agcctctctg aaagcttctt tgggtataag ttcctttttg
    14461 actctggtaa atccttctat ttcttcatag gtatagtatt gttcataaag ttcaagtgct
    14521 cttttgtatt ggagcaagag agactgttca gttatggttt ccctgtataa aaaagtatta
    14581 atatcttttc cgaactttac aatatctacc ccggaaaact ttattgcgtt agtgtctgcc
    14641 agtaattcgg cggcaatgtt gtaatagccg tcgatgttgt ataagttcag tgtcttcaat
    14701 atgtcgaggc tgagtttttc tatgcccaat acttttttta attgctcctc caaggtattg
    14761 aaacttaaat cctgagagta agcttttatg ctctcataat tgatattcat tccctgcatt
    14821 accaatcttc tcaattcaaa tccgtctact tcaacagttg cggtatccgc tctgcgatat
    14881 gctttgttgt cgtagaaata tggagtgtct tttccttttc tgaccgttaa aactatggtc
    14941 tctttattgt tttcactttt aagttgaatg ctgaaattcg gaatagggct tacggaatca
    15001 tttatcatat tttcaattct caataattgt gcttcagcat catccaaacc tgtaatgatt
    15061 ccgttatcat cgataccgaa tattattctt ccgtcattgt agttggcata tgcacttacc
    15121 gtttttaaaa atgttttgga cacttcctgc ttgaactcaa cattgatact ttctttcggt
    15181 tccattgttt tttctcctac ggacttatag taacatgatt gtagcagaaa tgcaacgata
    15241 agtttctcgt tgcatctctc gttgcaaaat gcaacgataa aaacgctctc gttgcacatt
    15301 tgaatgcaac gaggcgagct atctgaaata atgagcagtt gctttaatga ccgataatca
    15361 ttcctttttc tttatcttgt taagctttgc gttgaggtcc agtaaatcct cttttgtcag
    15421 cttcagcccc attgttccta ccatgccggc tagccgcaag accgtaaatc ctcccagcat
    15481 tttcatcatg gagtcattca tctcaaagcc tcccatgact ttctcacctt tttttggaat
    15541 cattttaccg aaaagaccca tgaaaagaag tgttcccctg gttgttttca atatgtcttc
    15601 tatcttatcg ttaagagaga aatatccttc gggcgcggtg atatcaaacc agttgagtat
    15661 tgcggtcttt tctctcatgc ggtaggattc atcaaacttc tcaaccttgt ttattacgct
    15721 ttcatccttt tgtttacccg ctacggccgt caatacggtt ctgcctttgt tgggtacatc
    15781 aaagtagaag aaacattttg ctttcttctt gcccaaagat acgccgtttg caagcagttc
    15841 cacttccggc tgattggaat aaacggtaac cttggtcata tcctccacac ggtttatata
    15901 tcgcttaccg catatgtgta cgaaaggctc atcggacaaa tatgccttgt atgcatagaa
    15961 agaatctttt ttgtacttac ggtctattgt taccaggcct ttgtggtttc gaccgtttgt
    16021 accgccttca tttctggcat cggcaccgaa gtcaaacata ttccatacat gagttgccca
    16081 taagtatttg cgtgaaaaca gttgttttat aagttcctcg tgataatatg cctgatactc
    16141 ttcggtatag tctccctgat cgggacggga ggtgtgccag ttcagtgctt cacatccgta
    16201 ttccgaacag cctatgggta tgtcggggtg ttttttatgg aaatcgtcaa accacggacc
    16261 gttcatggaa acatcaccgc cgtaccagcc gaaatagtgg ttgtaggata ccacatccgg
    16321 tatctttaaa taaggactgt cgacagggca gggggatacc gccgcaacgg ttgtcaagcg
    16381 tgtgctgtcc agttcatgac aaaggtcgtt gagtatctgg tgatttctta aaagatcggg
    16441 atctctgtct cccttcatgg ttatttcatt ggaaagtccc cagacaacga tggaaggatg
    16501 gttatagttc tgtattacaa gctctttcat ctgacttatc gtattttcat ttgcagcggg
    16561 attgtgttgt gaaatgtacg gtatttccgc ccacaccacc attccgtact cgtcgcataa
    16621 atcatagaaa tactgatcat gctgatagtg ggcaagacga atggttgttg aaccggtttc
    16681 gcatataagc tgcatatcct catcgtggtg ttcgggtaaa agagcattac ctatatccag
    16741 acgatcctga tgtcggcaaa cgccccttaa cggatattgc tctccgttaa gaataaagcc
    16801 gttttccgga tgtatctcaa aggttctgca tccgaaacgg gaggaaatat tgtctatgac
    16861 tttaccgtcc tctataagtt caacctcggc tttgtaaaga taagggtctt tacgaccgtg
    16921 ccacagatgc acattcttta tttccaacat ggcctcggtc ttatcggttt ccgattctgt
    16981 taccttttta tcttcggcat cataaattgt gtaacgaata gactgtcctt ctttgtgatt
    17041 tgttacccat gtttcaatat gaacttcggc atcttttcct gctatctttg gagtaatttt
    17101 tacccccggt cctccgtaat agtccaaaga gaaatgggat ttgtttacac agactaagtt
    17161 tacatcacga tacaatccgc cgtagaaggt gaaatcggcg aactgaggat aaaccttttc
    17221 gttgtgtgag ttatccaccg ctacaaccag cagattgtca cgggtaaggt gaggcgtgat
    17281 atccgctctc catgtggagt aaccgccgtc atgatgtgcc agatgttttc cgttaagata
    17341 aacatcggct gaagagtttg ctccctgtat ttccagatag tagcaatctg tcgtcggcag
    17401 atcggtttta tctatctgtt tggcataata gcaggtatta cgataatagt cgttgcctcc
    17461 gtcctgtccg tctattccgt tccaggtatg cggaagattt acccatatcc aattgtcggg
    17521 taattccttg ggaacggcat ttgcctgttt tgtaaaagcc cattttgcat ttaagttata
    17581 tattgttctc aattaactgc ctccttttct tcagcttccg cagcaacacg tacagcttcc
    17641 agttcctcat tcatctgtgc cagttttttc ttgtcaagat tgtagataag tcccagccct
    17701 atatacatta aaagagcact gaacgtgtat aaagcggcta cgagatagcg cattcttaag
    17761 gctacatcaa atgtctgatt gccttcattg gcttctacat atcccagcgc taccattatt
    17821 acaagagcta cggcaggtcc gactccctgt gcaagttttc tgaagaatga atgtaacgag
    17881 taggtaacgc cttcttctct ggcgccgtgc ttccattcat tgtagtcgat tgcatcgccc
    17941 atcattgccc aggaaacggt ggaatatact cctaagccta agctgcttat gaacaggcat
    18001 acgatataga ttatgagacc ctttaagtcg ggagtaatgg gcaggataat cattaagata
    18061 catgatacaa ggctgcaaag agaacctacc gttgccagtt ctttttttcc gtactttact
    18121 accatttttc tggctaaagg tgtaaaaagg aatatgggga gtaaggcaaa aagctgtact
    18181 attcccagta tctttacatt ctgaaaatat gactggaaaa gaacggtaac tgcggtagtt
    18241 gcaccttgga cgcctaaaaa cattcccatt gcggcaacgg ttgcacctac agcgggacgg
    18301 ttacgtatga aattggccag tgctttgaac acattgaatt tgggctgttt ttcatgaagt
    18361 ttaaccggtt cctgtactcg tattacggta tttttaatca taaactggaa tgctatgaat
    18421 cctaacactc ccataagcag agccgctata aatacacgga ctccgacgat gttattattg
    18481 ttatcgtaaa tgatagccgg taataaaacc atcggcagta tgttgcctgc cattgcaccc
    18541 accgaacgcc aagccgataa ggaagctctg tctgcgggat ctgaggatat gagagacagg
    18601 agagaaccgt acggtacgtt tgctatggta tagaaagcat cccaaaggac atacccgaat
    18661 acgaatatga gtatttttgc tatattgggt gcatttctcc acgggataaa gcacagcgct
    18721 cctcctacga taagtcccac ggagcctaca aaaatgtatg tgagaaattt atttctctta
    18781 tactgacgtc tgtcggcatc gataatggtt ccgattaagg ggtcattgat tgcatcccat
    18841 acctgcacta ttacaagcag tgctgcatac aatatggaat ccatctgcat gaattgtgtc
    18901 cagaaaatcg ccatggtgcc ttttaaggca aaactcatat tgcagccgaa atcacctgct
    18961 gcataagcaa tactgtcgcg aatgctgaac ttcctgaacc ctttctcatc taactgaact
    19021 tgcttttttt tcacgggaat ttcctcctta tttgtttgat gaaaggttta ttttgtttta
    19081 caacttctta tcttatttta ctataaaaag gctgaatatg tcattatttt taatgcgatt
    19141 tgatatcgaa tctgtttatg tatcggcatt ttttacaaag ttcttccgta atctgcctgt
    19201 tgttaaaacc gttaactatg tcaattgctc ttttactgcc gataatttcg gaaaaatcgg
    19261 tttttaaaat attgcccaag ctcattatcc cgtcctgatc cagacagcag gggacaaccg
    19321 ttccgtcaac caatacggca acatgattgc gcagtccgta acaaaaaccc gatgtattta
    19381 tttcttttgc atttatatcc ggccactcaa agacaaaatc ctgattgaca tatattctgt
    19441 cggctatttt aataccgcgg tcggttgccg atgcttcttc aatcctgaaa ggcaggctga
    19501 atgcattttc aattgcttgt aatacggcag agttttttcc tgcaccgaaa ctaaaggaat
    19561 ccttatcatc aagattccac agccttaaag aaataatcat gctcgtgtca ctgacagctt
    19621 gtttgataaa agttaatata ttcttaatgt aggtattgtc cgtattatta ttctccatac
    19681 tgtgcaatga aatatttatc tgccttattg cgggtttagc taataaggta tctttaactt
    19741 ccggtatcag tgtgccgttt gtcgttatat tcacaaaaaa ccctttttca tagctgatat
    19801 ccagaatagc acttaaatcg gggtggagaa gaggctctcc tttgacatga agatatatgt
    19861 agtcggtata gggttttatc tgatttaata tcttaccgaa agtatcgata ctcataaact
    19921 ccggcttgcg tttggtggca gggcagaagg agcaattcag attgcataca ttggttattt
    19981 ctatatatat ttttttaaat cttttagttg tcatcggatt ttcttgttgc aagtaaagat
    20041 tgaacttcgc tttccgtaag gtttctccac tttccgggag gaagattctt taaccctata
    20101 tccattatgc gtatacgtct gagttttacc actttatagt caaaagaagc acacattctt
    20161 cttatctgtc tgtttaaccc ctgcgtaaga acaattttaa acacataaga atccagtttc
    20221 actaccttgc agggttttgt gcgggtaaag gtgtttttta ccgggttgaa tatcctcact
    20281 cctttggaca tcttttggat aaaatccggt gttatctctt tatccaccgt tactatatat
    20341 tctttctcat agttgttctt gtctcttaaa atttcgttta ctatgctgcc gtcaccggta
    20401 agaagaatca aaccttcgga atctctgtcc agtcgcccta ttggaaatat cctttccgga
    20461 tgattgatga agtcaattat gttgtttttt acatgtcttt cggtggtaca tataactccg
    20521 acgggtttgt taaatgctat gtacacgaag tttttagaag gttttacata ttcaccgtta
    20581 acttccactc tgtcgccggg attgacaacc tgacctataa cagcagtttt accgtttatc
    20641 ttgactttac cttgtgtgat aagttcatcc gccttccttc tggagcatat acctgcggaa
    20701 ctgatatagt tgtttattct cattgattat tcctcataaa gcaatcggta ttgtagtata
    20761 gaattatatc agaattgttt cagtgttatt acaaaaactt atttcggtca tagaagatat
    20821 ttatttcaaa taacttgggc ggtataagaa gtgactacta catatgcttc cgataaatat
    20881 acaggtgttt ttccaaatca cgacggccat ataagatacg tcttacttcc atggtgtctt
    20941 cagaaattac ataaaaaata atatagtttc ttacataaat tctatagtaa ggtagaggat
    21001 gttctttttt tgaaggaaag acttcaaaac tctccggaca ggaaaggcgt ttttctattg
    21061 ctgcatatac ctcgtctact aatgaatatg cagcaaccgg attttctaat tggatactta
    21121 tgtaatcgat aatttgattt aaatcttttt caaaaagggg aagaaatcgt agcctatatt
    21181 ttttcatgtt ccttaatgcg ttttcttgct ctgtcaaaca cttcttttgc agtatatcgt
    21241 tctgtagatt ccgctgccat gaaatcggct tcatccagtt tagtttcaat gttttccgtt
    21301 agggcagtgt actgctctaa actgataacg accatggttc catagccgtt ttttgttaaa
    21361 taaaccggct taccttcttt aacggtttct tcaatttccg tgaaacggtt acgtaaatct
    21421 gatacaggac gtataactat cattaaaatc acctcatttc tgatggcgta atattatcat
    21481 aattgcgcca tattggcaag agaaatgttt aaatacggtt ttaatcacat tattgtaata
    21541 tacgtatagt ataagcatat ttattgactt gagtgcttaa ataactatct tgatataaat
    21601 cttctccagt ttccgtccga caccgatatc cctttttctt cggcatattt tttgttagcg
    21661 tagatgtata cccaacattc ctgttgtttt ccgtcttctc tcaaaacggt tcttttttct
    21721 ctgatatata aattattatc cgctgtgccg gtgtaaccct caaatttatc caggtcagcc
    21781 aaaagcaaac catcgaccgg ttccataatc tcgccttgga ctgttccgtc tccgtctacc
    21841 aaagcgggat aaccttccgg aagatgatag agggttccgt gtgtttttcc ataacttata
    21901 ttgcttatct ttccttcaag atattgctta tagaacctat aatctttcat caatgatccg
    21961 tatacaaata ttctgtctat tttcatatgc accgccttac tgaatatccg catcttccgt
    22021 aaaggggatg gaggattttc ggaatatctt tctatttaat aaccgtataa tccaccgcta
    22081 ctctgtccaa aagtctttgt ccggcttccc atatgtaaat ctctttttcg tagaatgata
    22141 ctttgatttt ggttccttgt gccaaatttc tcaaactttc aacatccatc tggcctccga
    22201 tgaaaaagga atacaggaat ccgtcattat ccaggaaatt taagtattgg tagtctccct
    22261 cggacagttt atagaagatg ccttccactt gctttttctc actcacataa agtttcctgg
    22321 tggtaacccc gtttgcaaaa gtattgtcat ttatttcata atggctctca aaacctacta
    22381 caaacttata tatatcggtt tttcccgttt caatgttggt taattgctcg tttcgccaga
    22441 tgacataggt agcctgattt ttctgcaggg tagtgtaagc aaattcggtg caaacaaagg
    22501 tcagggtgtt accgttttta tctacggtat cgaattgagt atatgaagtg tatttactca
    22561 gagggatagg tttttcatcc acttttttca ccaacgagat aaaatccgac tccatgtcgg
    22621 tatttatttc ctccgcactt gcttcctttg tcaaatccac atggtcggga atgacaacac
    22681 cggggatata cggtatgccg tccgggctat agaaataatc gtctgaaggg gcttctttta
    22741 atacctcata ctcatagagc cgtcttaaag gaataagctc gccttttgca tcatcctcac
    22801 cgaaatcaaa aaatatacga tcggctttta tataccattc tattttcact tcggtacccg
    22861 gttctccggc ataaagcgat atgtaagtat ctttgtaata aaagtcgtac agataaccgt
    22921 cctcacccag tacatacagg actaagaaaa gatcatcaat tctttcggag agttttacga
    22981 cacccgatgt tattctgata tcataatcgc tgttcaactt gtacgatgtg agtttgcctt
    23041 caacaaagtt atcgtcctga ttggtttctt catctatata catatcgtac gcacaacagt
    23101 atagttcctt gtcgttctca tcataagcat tactgtcgaa gtaccagatt acattgacaa
    23161 tctcacccat atactctttg aagaagttat aaaactggta ctcggtagga taaaatttaa
    23221 gagtttcttt gtttgcattc cttgccgtta cttccgtacg ataccctaaa accttatctt
    23281 ctttatatga aataagtctt acagtggaga gatagtttgc cgaataatct tcgtcctctt
    23341 gatatttctt tttctccgca gcgaaatcca attcctccgg ctccggaaat tcgggcaggg
    23401 gcgccatctc atatgttaca ccgtcaattt ccatggtttt cggttggggt aacggttctt
    23461 ccgaaggtac agggtcttca acaaccggtg tacccgtttc cgaaggagca ggtgtttccg
    23521 aagctgtagt cggggcaaga gtaactacct cggaagggcg gggtgttaac gtaggtccgc
    23581 acgatgtaag caggagcaga aggaccatga aaagaattgc aagccgtaaa cttttattct
    23641 ttacggattg cacatcaatt tgtcctgacg attgaaatac gcggtttata tatttcattt
    23701 aacttaacct ccacggaaat gtcattttgt cattgatatt ataccatgca ttgcgttatt
    23761 tccttctctt tagtttgact taaacggaaa gatatagtca ttgtcggaag gattttatga
    23821 ttcatctttt acattgttat acggttcact gttttcattg catgtagata caaattccag
    23881 ttttagttgc atattcaaac ctttggctaa tctcttcatc atattaagac tgggatttct
    23941 cgttccgttt tcaattctgc tgatatccgc ttgtgttatt cccgttcttt cagacagttc
    24001 tttttgtgta aggttctgtt ggtttctggc attaatcata gtttggataa tgtcaaattc
    24061 aggctgcaaa gcatcatatt cagcttttac ttcaggattc tgaagagctt tgtatttata
    24121 ttctttgtag ttactcattg tcatacctcc gttcataatc tttcttatac tttttagcaa
    24181 gttcaatctc atgtttaggt gttttatcag tcttcttgat aaagccgttt gtcagtattg
    24241 ctttgtgacc ggaaataaag aaatacaaca ctcttgttat atcgcaccat tgctgtgttc
    24301 taagttcaaa taatccttcc tcaacagctt tggaataggg acctctaagc ttgtgtccgt
    24361 atatttccgg taaatcaatt gctctcagag ttttagctaa aagcttgctt aggttctaaa
    24421 gaatcaagaa agtctttgac cggacacact ccatcttccg tattgtagaa aataacttca
    24481 aacactcata ctctcctatc tcaatatcaa ttatatgtcg tatacgacat attgccaata
    24541 aaaacagtat tgtttaaatg aaaactgata acggtaaatt gaacaatctt tttaaaaatt
    24601 aacttatttt caatagtttt ttaaagcaga tgacacaatg gtttttttgg caacgataaa
    24661 cacctgaagc aacataattt ttgcatatta tgatgaaatt tatttacttc aggtttttag
    24721 ttttaaatgt tctaatcaat taccgaaaac tctaccgctt gtctatccag gattctgctt
    24781 cccgcctccc agatatacat ttccttctcg tagaatgcta ctcttatttt tgtgcctttt
    24841 tttatttcaa ataaatcatc ggaactttcc atcatacccc atatgaaagg agcatacata
    24901 aatccgtctt cacctacaaa taccaggtaa tagtaatctc ccatagccag ctcatagaat
    24961 gtgccttcca ttactatttt atcacttgta taaagtttcc ttgtggaaac aacatattca
    25021 aaagcatctg tgtccaccga atagtcttca atacctatca catatttata tatatccgtc
    25081 tttcccgttg ccttatctgt caattccctg ttttgccaaa taacacagac tatcgcatct
    25141 ttctccaaat tttcatcatt aaaatgcgag tatgcaaagg tcaaactgtt accgtgttta
    25201 tctacggcat caagcacggc aagatcatcg taataatcaa aagaagcagt tcttttttct
    25261 gcaattttta caaacgaaac aaaatgagat tccatgtcgg tatttatttc ctcagcggtt
    25321 gcttcaattg ttaagtctac atcatcgggt ataacaaccg acggagcata aggaatgcct
    25381 tcctcgcttt ttcccaattc ctcaatgtat gccgattgtc ctataagctc aatatccatg
    25441 actcctctca cagtgagaag aacgggttga gaatccgaat tttcatcgga tttcaatatc
    25501 agagtttcta taatccaatt cattcgcact tcatgaccta ccgttttttt atagtcatac
    25561 agtatggaat catctaaata gaaatcatat attttaccgt cattgcccag tacggatatg
    25621 agcctgaatt cgtcgtcaaa catttcactg tacttaataa caccgtccaa tgtccgcgga
    25681 gtagtgtcgg tgtataatgc atacgatgac agtctgcttg tatcaaagtc atctatgaat
    25741 tcatctacaa ataaatcata tccgtaacaa taaacttgct tatcttcact gtaattatct
    25801 ccttccagaa agaaccagag gatattaaca atatcaccca tgtgagattc aactttttta
    25861 ttaaaagtat cccaataaaa ctggaatttg agttcctctt ttttggcagt tcttcccgta
    25921 acttcaatat atccttcata ggcattggag ttggtatagg acaccagcct tacgttggaa
    25981 acataatcca cataatatct tccgtcgctt tctatgtatt tgcttttttc cgcagcaaaa
    26041 tccagttctg tctgttccgg catatcgggc agcggggcga cttcatatgt tacaccgtct
    26101 attactacgg tttttacttc gggtaccggt tcctcgggta gcggctcttc actgggcaca
    26161 actgtttccg aaggtgtagg agtttccgaa ggtgtagtcg gggcaagtgt aacaacttcg
    26221 gaagggcggg gagatacatt aggagtgcat gatgtaagca gcaacatcag aaccataaaa
    26281 agcattaaaa gacgcaagcc tttattccgt accgatcgag tttcaagttt tcctgacaat
    26341 ttaaatacac ggtttatatg tttcatttaa ctaacctcca agggcatgtt gtttttcatg
    26401 gatattatat catgtaattt ggtaagaact ataacagtga tacttattta acaggcttta
    26461 tacacaaatc tcaatcaaat cattacatat catctttttg ttaattgtaa ggatattaat
    26521 gcttcccgac atcgggaatt gcctgtttac cgacatagtt taaacatata tgatagaatt
    26581 catattactt tatcgggttt tatgaaaaat ataggatacg accccgatta tataaatcct
    26641 ttgcgtattc tatatacgag aagagggtga ggtatggaaa gagagcatca gattgtaaaa
    26701 gcggtctatg cggcaaaaga aaactctcaa aaagtcgatg atctcataag agattatatc
    26761 cctttcatac gctcggaagc ttccaaagcc gtttcacgct tttgtaccga gcaggatgat
    26821 gaattcagta tagccatgat tgcttttcac gaggcaatac tgggatacga aagggaaaga
    26881 ggtgcttttt taaattatgc gtctatgctt attcgcagtc gcattattga ctaccaaaga
    26941 aaagaggcaa gacatagagg cacgatatcc ctgtacgaag aaaccggtga agacgaaaga
    27001 acattaatgg atgaattggc agatgagaaa gactattatg aatcttcagc caacctggaa
    27061 gccacaaagc aggaaattga agaattggca gccacaatag cacaatacgg agtaagtttt
    27121 tccgatattg cggacaattc accaaagcaa ggtcgtacat tggaggcctg tgcgcaagcc
    27181 ataagttacg gagcacaaaa caaagaaatt ctggataagt tgaccaaaac caaaaaactg
    27241 cctctggcag aattggtcag aggatccggg gtggaacgta aaactttgga acgtcacaga
    27301 aaatacatac tggtaatgct tttactccat accaacggat acgaaataat tcgcggatac
    27361 ctcaggaata aaattgtaag gaaaggagga agtctcaaat gaaatacatg gtaatggaat
    27421 gtcatctcag ctatgcggtg gttttaagcc gggacggacg cttcctgaaa gtcgccaaca
    27481 gacactatga agtagggcag actgtaacaa atgtaattga gatgcaaatt cctcagtctg
    27541 ttcccaaaga aaaaacagta gtaaaagaaa gagagagaag gcggtgggta tatgctgcgg
    27601 tagccgtggc agcggttttc ctgttggtat tttcctttgc actgcaaacc ggtctgtcaa
    27661 cttttgcttc catatattta agcataaatc ccgaagtgag aatagatgtc aacagatacg
    27721 ataaagttat cgctcttgaa ggcataaata ccgaaggtaa gaaactggtt gaagaatacc
    27781 actataacaa taaagaactg aatcctgtaa ttgacgaact tgtggacaga gccatagaga
    27841 tgggttactt gcacgagggc ggccggatta ccttagtttt ggactccggg agtgatgaat
    27901 ggatagcaac tcgcggagtt accttgggtt ccactttaac cgagcatgta aaagaaaagc
    27961 tttcagttac gattgaaata agtgataaga ataatccgga agataaaata gtaattccga
    28021 tagatgacga tgaagaaagt atacataatg acacggaaga tgactatgac gatattgatg
    28081 acgacactga agataatgat attgatgata ccgataatga tggcgagtat gaatcggatg
    28141 atgacgaaga aacggatgac gatgatgacg agtatgaatc ggatgatgac gaagaaacgg
    28201 atgacgatga tgacaagtac gattcggaca atgacgaaga aatcgatgac gacgatgacg
    28261 aagagaccga tgacgacgac gacacagatg atgacactta ctcagacgac agcgatgatt
    28321 cttaaatgat attatatagt cgttttttta atgatgcatt ttatgtgttt catgggcaaa
    28381 aaagacaccg aactttgaaa atatatattg cgaccccgat atcgggaaaa ctgttgcgta
    28441 atctacaagt gaaaaggcac attgcccgtt gaatcaaaaa atgaaaggag aatgatttaa
    28501 tgaaaaaata ttcagtatta aaaacattat caattatttt gagcctggtg cttataagcg
    28561 ccgtagcact tgtaggatgc aatttgagaa ggcctgacag tgacgaagag aaagttgttt
    28621 ctgtcggaac tttattactc agagttaacc ccgagatagc tatagaatac gataacaacg
    28681 gtatagttgt agcgttacag gcaaaaaatg aagatgcaaa agccatcttg aaaggtaaag
    28741 aaggtttagt aggtaaagaa acccgggaag tgatatcaaa gcttgttgat gctataggag
    28801 aagccggtta ttttgtagaa gaaatagaag gggaagcacg tcagattacc attgaaatca
    28861 caaagggttc taaattgcca tatgaccaat ttattgaaga agtggttgcc gaagtccgca
    28921 aaagtgtgga agacaacaat tggaaagctc ctttaggtgt caaaaacgac agcgacttcg
    28981 gattagcgga ttacaatgac aaggattatc atagttcaga cgatgatgat gacgacacgg
    29041 atgaagacga cattgacgat gatgatgacg atgatacgga tgaagacgat attgacgatg
    29101 atgacgatga tacggatgaa gacgatattg acgatgatga cgacgaagat gatacggatg
    29161 atgacatcga tgatgacgac gaagatgata cggatgatga catcgatgat gacgacgaca
    29221 ctgacgatta aaagacaaag cctttataac tctttaagga attagaacag ataattaatc
    29281 ctcaacactt atgaaaaata tacaggcatg atgcaaaagc agactcacac ggtaactacc
    29341 gtgtgatatg ctgttactag agaacggtta tactattctt taagtctgcc agattcttct
    29401 catggtcatc cttatttaca tatgccactt tatcgcttaa gaattccaca tccttaccgg
    29461 ctttatgaaa ttccagagcg gtttctctgc aacagtattc cgaagctatg ccgcacacta
    29521 agattttctg attcacaaca tctttaagga cctgtccttt tgcattatta gcctcaaaag
    29581 cggaatattc ttcaacttca tcatcctgac ctttgtagaa gatattgttc ggattgggtc
    29641 tgttttttct cttctttatc ttactgtaaa aatctttatg gatttccgat cctcttttat
    29701 cttgaacaca atggtcgggc catattcctc cgtgcctttt aaaactttta ttattctccg
    29761 agtgccaatc acaactgtag tatacattta acttcggatt ttcatttata aagtctataa
    29821 tatttttcac ggcattttcg gcattgatac aggctaaaga gccgtcaata aagtcatatt
    29881 ggcaatctac aactattaat gatttgttca tattaacctc caaacaccgt taattatatc
    29941 atcccgaact gtctattgtt ccggtttaat aggctcaatc tcggtatttc cgagtttaag
    30001 gatgatgaca cctttacgaa ccgttatgtc aaatatgtgt gtttcaggag ggctattctg
    30061 atttacacct tctatttgta atttaccgtt ttcggcttgt acaaatctca gagggtgata
    30121 ccattgtgtt ctatacatat tgtagtattc aagttcccgg ttttcgtagg cagttaaagt
    30181 aaatgtaaaa acacctgatg tctgtcccca accgaggtaa cacttttctt cttttccgtc
    30241 tttgtcaata tcacaaaagg caacaccgta tgatgctctt tttgcatatt cgctatcaac
    30301 cgttcggctg taaatcatat tacaattatt atacggttta tatatagaag aaattaagga
    30361 gtttaaatat ccttccgcag tcgcatcttc actgtcggag aggagatagt attcggcagg
    30421 tatcggatta actttgctcc atgaataaat gctgcgattc agatatttat attctccttg
    30481 aaacaaaaat tcctcttttg tgtcaccggt cctgtcgtaa tagctaagca gatatttttc
    30541 tccgtcatat ctcaaatctt taacccggta actattgtcc tgcatggaat aagattgata
    30601 aatacgaaca tgagcctgtc gtccctgtgc ggtattattg acaaaatcca cccatatttt
    30661 ttctcctgca agcaaggtgc tgttatctat aacgacataa ccgtcggcgg ttgcctgttc
    30721 tgcggaatag ttttccttaa gatcttccag tgcaagctta tagtcgggtt ttatggcttg
    30781 ttcggtcagg gaacaagcgg taaggatagt gcaggtaata attgtaacga ttacactcca
    30841 aaacagcggt tttctataac tcatagtgtt tttatccttt ctccttactc tatagacggg
    30901 taaaaacaac aacaggttcg cctctattac gagaaatgtc atattcattt caatggaata
    30961 tggcataatg tgacaattcg gatgatagtg gtgaatttag ataaaacgct cttttattga
    31021 tgtattagtt gacaatgtaa actatatgta ttattattag catgaaagga gtgatattta
    31081 tggcaactgt tcctactcaa attcgcattg atgcggatgt gaaaaaacaa gcaaacagcc
    31141 tttttaatga actcggactg gatatgtccg gtgccgtcaa catgtttctt catcagtgta
    31201 ttttgcgtgg aggattacct tttaatgtag agcttcccaa atacaatgaa gcaactctgg
    31261 aagcaattgc ggaagcaata aaaatatccc gtaatcctga cataaaggga tatgacacta
    31321 tggaagaatt aaaggctgct cttttgtctg aatgatgtat aaggttaaat ttacaacagc
    31381 ctataaaaaa agttataaac gtattatcaa gcgaggtctt gatgtttctg ttttggataa
    31441 agttattgat gaattacgtc aaggtaatca gttggattca aaacacaaag accatatgtt
    31501 aaaaggtgag tatgcaggct tccgtgagtg tcatattaaa ccggactggc tcctgattta
    31561 tttaattgaa gacgatattc ttacactcac cttggtagat acgggaagcc attcagacct
    31621 gtttagacct taaagacaag tccgagaaac aagatataaa atcagtttcg ctccgggttt
    31681 tgtcaaaata gtatataagt tatttttctg tcgcttgaat ttgttagcag tttcataagt
    31741 attaccgcta atggtcatta tgcgttttgt cttggtacat cctttgatta tccggcattt
    31801 tcgcgggtat ggcagtccta tgctttttct tattttcata catactgtga tcaaccctta
    31861 aaaaggtagc atgaatatct tcatccaaag agacatcata ctcagcaaca cctaaagata
    31921 ttgaaaagtc atattcgctt tccttatcta ttaaatccac ttccttttct aagtttttaa
    31981 ttatcgcata tatttcattt atgttaatct tttcaactat tacaacaaat tcatctccgc
    32041 ctatgcgata aatcctgccg atgtgaccga agtttgtgtt caaagtattg taagtatcga
    32101 taatagcttt gtcaccgctg aaatgaccga agtaatcgtt tatatatttc attctgtcaa
    32161 tatcggccag cacaataatg actttttccg gtttagtaat catatccata tcttccataa
    32221 atcttgcacg attacccgct ccggtcatgg catcggtata tgccattttt tcatagtatt
    32281 ctttttctac ccgtatcctt cttctttctc tgtaatcctt aacctgataa atcaatacta
    32341 agactatata tattaatacg ccgatacgca gatagagaga ggaaacaatt tgtccgttta
    32401 caaaatatga tataatctcc gcaactgcaa atatgaaaat aacggaaagc gatttcagtt
    32461 cccgcaaagc tttctcgttt ttgtaagcaa ctgattctat gataaaaatc gccatatagt
    32521 atacacacat taccgatata agtcccaccg caactaaaac cgtatccgaa agacctaata
    32581 tgcctgaatg ctccagcccc aggctgacga ttgtatttat aattattaag cctattaaaa
    32641 agtcatcaaa aaacctcttt ttcataggaa cggcttccct tatatacaaa ctgacgggta
    32701 taggagaaac caatgtcatg agatacgcaa ttctggtggc ataatacgta tttgcggtaa
    32761 ataactgcaa catttccgaa tcacctaaaa accaattact cattagtaaa gcataaattc
    32821 ccaaataaag gagacttaga tttttctcct tatacatacc caggaaaaag aaagttaaaa
    32881 taatcattac tcctaaaatg aaaataatag gagggaaaat attccaaaga ccatgttgag
    32941 caaaaaggta accgtaaaag gcactctctg aacctataaa aacctccgaa gcgaaaccgt
    33001 gtgaggcggc tctgtgggag aagagagaaa tttcaatttc ctttccttta tgttccgatt
    33061 gagtctttac aactgcccag tagttaccta aatctcttcc cataaactta cttctgtcgt
    33121 tgccgatatc atagacggtt tctccgtcaa tttttataac aaccgactga acattggctt
    33181 tcaaagcaat aaccgatccg gcggggaagt ggtcgggaac agtgttcttt atcactaaag
    33241 gctcggattt ttctacatca aaacgatgag gtaaatagta tctttctttg taaccgtctc
    33301 ctttccatgt ccaaccgtct ataaaagaaa ctccctctat gtcaatgaga taaagattcg
    33361 gcatatccaa aacatcggta agaacaaatg tggaaataaa tatagcggct attagtataa
    33421 aaaaagttat ctcattaacc tttttcaagt actgttcact ccctctcgtc gctgtcatat
    33481 aatgacagtt atattatgac tatgatggta gggtttgtca aacaaggctt accgcttagt
    33541 ttttttgaga taaatgatta gcattatcag ctttatttgt tttcatgtaa ttatgtcaaa
    33601 aactacaaac agtatcccgt tcagtcaaat taaaattaac tgaaatcgaa agagaaaatg
    33661 tgtatcttat catcaaaata ggcaccgtta tccgaccagt cggataaaag tgtcagtgta
    33721 agccgtttta ctttctttcc gacatttatc ctgattaacc gtttgatgtt ttcagtgtcc
    33781 tcaattaaaa ctattttgct gccgtcttca agctgtgcgg tcagtttata tgattttacc
    33841 agagtcttgg gcatcgtcat aacgggtgca tctatgttta tacttgccct catggtatgt
    33901 gtccgttcgc agagatcgcc gggtaatgta tttctgtcca gatcggagtc gaataccata
    33961 cggacttttg aaatttccgt cggcttttcc aatgtatatt caatagttcc gccctttcct
    34021 atatatactc cttgaggacc gtcataaatg tggtggtcgc gatctattcc gctcctcaga
    34081 ttatctaagt tgtcaacatg agggtcggat gttaatgccg cttttttgca taaaggagaa
    34141 atctccctta aaaatcccgg caggaagcag tcgccttcca ttaagttttg ctgaatctcg
    34201 gaaatatgaa acttgcgggc gagtctgggt gtcactttat actttttagc tactgcacaa
    34261 ccttcaccga ctgcctggcc caacagagca caggttgcca taatacgtga agatgacatt
    34321 gcggcatgcg tcatacttac atttcttccc gcaaaccaga gattttctat gttcctcgaa
    34381 taaaccaccg agagaggaat tttatacggc ttgggagtag ggattattga acttgcacgc
    34441 ccgggatgcc agaaaccgcc gggatcgtga tcatctaacg gccagccgcc gtaagctatc
    34501 gtatcgggga aaaaggtatc tgccgttatt tctttctgtg tcagtatgtg atcacccata
    34561 agcctcctgg attctctctt tcccggcaga aaacctatga aatccagttg ccagcaacct
    34621 gcatcataat ctccgctgtt tttaatgtaa tcccacattc cgtaggcaag ggctacaagc
    34681 tcatcgcgga tggtctccgc atctttgata gtgtctctgt ctccgcccaa ctccagatac
    34741 cagaaatttt cataactgct ttcaatattc gggtgccgtt tttccacatc ctcccgtgtc
    34801 agtttggttg caaacggagg ggctttgaag gttacttcat gatccagctt tctggcttga
    34861 atcaggcagg aattgcccat ggttttgcta tccggttcgg ttaaagatat atcttcatcg
    34921 aattcctctt tagactctcg tcccatccga tattcggctc ccgtaagcgg tgctaaaata
    34981 ctgtcacccg aacagtcggc gaagtaatct gcttctactt tctgccatgt ctgtgaggtc
    35041 atctgccagc cgtatacggc cttgatttta tctttgtcca tatccgcatt acagcaggaa
    35101 cagttaagca tgagtgtaat attcggttcg gaaataacca tattgtaaat taagctgtcc
    35161 catacggggt agagcttata cgggttgcga tacagcgatt ccagctgcag ttcttcaagt
    35221 ataccggttt cacggttatt caagcctttg cagccgcata tccacatcct tatctccgaa
    35281 gaagcattac ctccgagcac cggacgctcc tgcattaaaa ccacttttaa tccgcgtctt
    35341 gcagctgcga ttgcggcaca tacgccgctt ataccgcctc cgaccacgca gaaatcacac
    35401 ttggttgttt cggttattaa tttcatgggt ttatcctcct gataatcaat aaatttattc
    35461 tcagtttatg ttacctatat tatattgctc tatcgtgttc ggtgcaatga atgaacagtt
    35521 aatttaagaa taaagcaacc agaaatatat gtatgcggga attaccgctg ccaaagtaaa
    35581 gggaataccg atacggacga aatcctttgt ttttacctca tatccggctt tccgtaatat
    35641 accgataccc gcaatatttg cggaggcacc tataggtgtg cagtttccgc cgagcgtggc
    35701 accggagagc agtccgaaat agagcagagt cgggtcgatt cccagattgg cggcaagacc
    35761 gctgataacc ggcagcatag ttgcgacata gggaatgtta tcgataaatg cggaaaacaa
    35821 aaccgaagcc catacaatga ccgtataaag aaggaaaacg tttccgccgc cgagttttgc
    35881 caagagtgcg gcgacggcat caattacgcc catatgggaa ataccgccga ttacaaggaa
    35941 gagaccgaat aacaggccta tggtttccaa gtccaccgct ttcagcattc ttcctatcaa
    36001 tttcttatcg gaagttttta tggcggtata aataatgccc gcaatcagca atccgacaca
    36061 gataagaccg tttgtaatgt ccggcttatt cggaatgaaa gatgccgcaa tcagaagaac
    36121 aatcataccg gccagcaaca cggtgggaac atagtcggta acaacggtac gggtagtatt
    36181 cttgggtatc ggttgctttt ccttgcggaa cagccatgcc agaatcaatg ccgaagcaag
    36241 agcgcctaac tcaaccgcaa agaaaatacc aggcttaccc ttgtaaaaga agaagtccat
    36301 gaaattcatg tttgcgtacg agcccagcat tatagcggtt gtatcaccga ccaaggtcgc
    36361 tgcaccctgc agattggagg atacggcaat ggcaatgatg aaaggaaccg gattggtctt
    36421 cagtttttta catatttcca gtgcaatggg ggctaccatt aaaacggttg cgacattatc
    36481 cacgaatgcg gagatgattc ctgcgaatag tgccagtgca acggcagcca tctgaacatt
    36541 gggaaccttt tccataacca aatcggccag aagcgcaggc atcttactgt caatgaacag
    36601 ctggactaaa cccatggtac cggcaatcat caagagcaca ttgaagtcaa tggaaccgaa
    36661 aattttgtta agcggcagca ttccggagat aataaaaagg gctgccgaaa tcagtgctat
    36721 atatgtacga tactttccta aagtcagcat cagtatataa gtaatgacaa aaagaataat
    36781 cgcataaatc ataagaaaac cttttctcct tcacatcctt ataccgttaa cggtctgtca
    36841 ctacaggacc cgtataatta tactacaaag tcataaaaac ggtttccgct caatcacata
    36901 accgtctctt taaaagcaat ttttattggc tctttttaac cctgcgagtt gttttcagat
    36961 aataaaatgt gctcttcaag tagtccgtct tctgtcggga attactcttt cggcaatatt
    37021 tctcttctta tcttcataca tggaggtatc caatttcctt aagaaatcat ctgtcgactc
    37081 atgctcgtta tcatatgtgg aataaccgat tgaaaaatgt attttatatg gtttgtttga
    37141 cgattcattg aactctcttg tatgtgcctt tatagtgtta accatatcaa taatttcttt
    37201 ctcggatttt gcatgcatca atatgataaa ctcgtcgcct ccgtacctgc atgatacccc
    37261 tttatcaccg acggctgtat ataataatct cccgacttcg gaaatagcat catctcccac
    37321 ggtatgtccg aattcgtcat ttactttttt gaatccgtca atgtccaaca taattccggc
    37381 aagtacaccg gaagcatctt ctcttctgag gcacattaaa aacaggctgt ccagataatg
    37441 tctgttaaac aatccggata gcgcatctat ataggctgct tcattctgaa tattgaggaa
    37501 taaagaaacc aatcctattg ctacaccaag ccaaataaga gagtacccgt aaaagaaaaa
    37561 ttgcaacagg ctgccgataa tgataggaat cataaaaaga atggcaggca taaacaaata
    37621 cttttgtacc ttattgcgat ttaaatagat taacagaacc ccgtatgcca tataaaaata
    37681 ggtgacggca aacggaatta tatacaaacc ggttctttga taaacattat attcatttat
    37741 tgtaaaaaat acgggtgtca cgaagtttat tatgcaacag attaaaacca gtatggcagg
    37801 tatacccaca taagggtata tccgtctgat tcttctcata tccccgaaca atttatagtc
    37861 cgcatatatt acccaaagaa aagcaaagat aatatcatta atgtataaag cggcattgag
    37921 cactatcagc aatgtatgac tgccatatgc catgttgccg tccagtataa aaaccaccgt
    37981 ctcgatacag cattgtataa tattgaaaat aaccattccg tagaaaagtc tttcgtcaaa
    38041 caatctggta attgacggtc tttttgaact taataagata agcgataaca acattactgc
    38101 tgcaccgttt gctatatata tcgccggata attaatcatt acaaaaatct ccttcccacc
    38161 gtactgcaaa ttctcttaca gcatattctg ctgatttaat tatttcccaa cctgccatcc
    38221 tacaaataag cgtactaagt tattagaata attataatac gtagtcctaa tagaaacaag
    38281 tacaaaatgt ttgtgttaca tcaatatttt gaaaaattga cacagcgctt atatgtggta
    38341 tctacatctt atacgccgaa atttcattat ggctctgaag atatttaccg tttttatgtt
    38401 ttcatattta aattccgctt cttcttatgg taaaataaag ataataaaga tttctttgtt
    38461 tacttgatta atttagaaag gataatgatt atgaatttag caaaagtttc atccagtggt
    38521 cagattaccg taccggttga ggtgcgccgt gccctcaatc tgaaaccggg cgataaagtt
    38581 ctcttcattc aaaacgagag aggacaattt actatcggga atgcctcagc acaagctatt
    38641 tataaggcac aagctgcctt tgaaggagtg gctgagaaaa taggaatcaa aaacgatgat
    38701 gatatacaag ccttggtaaa cgaggttcga tacggaaaat gaggatactt gtcgatacca
    38761 acatacttat tttcttgcct tataatcaga gcatcctttc tgtataacag tagctcaatt
    38821 cttagattat tattaatccg catactcaat aagttgcatc atagaccgaa cagtttcgat
    38881 gcatcaaaaa agatgtcatt tggcaaacag actttatgtt ttttaacaaa agacctaata
    38941 aactcatttg tcacttgttt atgtttctca tattcccgtg caagctcaat ctttatctct
    39001 tcacgttttg attcggcatt ttccgcttct acataaataa ttccgtaaaa tctgtcatat
    39061 gaaaagtata tttcatacat tccatccttt gaggaaaata aacttacttc gactgcatca
    39121 tcatatactt cgttaatttc catataagta ttgctataat gcctttgctt ttccgtaaaa
    39181 aaatccgtca cattttccca tttatacaaa tctaaatacc ccgctttcac acatccttcc
    39241 tgcaaattaa aaatcaaaat ctttagttac agcaataaac aaatctgccg accaaaagtc
    39301 aacaactttc ctgtcacgaa taaacggaga aacatcagat tttgccgatg cataatctat
    39361 tgttgtgaac ctttcacaaa gcaattgctt aagaatatct ttgtcgagcg attgataaga
    39421 tttcagatag ccgttgttct tcagcctgct ttccagatgt gcgagattca taggatattt
    39481 gttagttatg taaaaaagat agtcatagag atcgcgcccc ttgactctgc ttttccaact
    39541 tcgcgaaagt acggcagaaa ctttcccggc aaacaatgtg catttatcgt aaagtcttac
    39601 aggggtagga tatggctgta gcagatattt cgtttcatag cttgcagcgg aaagtgctgc
    39661 tgcatccgct tcaagtttta tcttcaatac ttcgttagcc ggaactcttc ctgtaaagac
    39721 ctcttcggtt ccgaaacaat ttaaaatttc cctgcaattg cctttgatat atcctctttg
    39781 tataaatccg cttttcgaat caacttcaaa tgttgccgga ataccaagag aagcaagctc
    39841 attgttgata cagccgacat acttatcaag tacgaatgta tctatctttt cgttatcgat
    39901 agaaaaatcc aaatcttctg aaaatctttg gagaccgtga aaaattctaa gagccgatcc
    39961 gccgttaaaa gctgcattct gatagaaatc agaacgtccc agtccgcata atgtaatttc
    40021 ctgcaaggct tgcttaactg cgttttttgc ttccgcatcg cttgtagcct cgggatattt
    40081 actcatcatg gaatctaata cactattcat ttttcggtct cctttgcatg atatttcaaa
    40141 agataatcca agttgcgttt attgtatttt ggggcgatgc ttttaataat ctcccagtca
    40201 agcttgttca gttcatactt gtctatacga agattatcaa acatcaattc ctcaaatgcg
    40261 gatacggagc taacctgcgg aatgctgtac agtttgtcgc atagtgcctt ttcgggagtg
    40321 gcaattatgt atgaataacc gtcttcaatc cttgctattg ttgcaagcgg aaatacctca
    40381 ggcggaatat ccctatagat gaaagaaccc aacggggtat catattgctt aactttattc
    40441 ttcctgaagg tggcgcatgt gatatttgga acatattccg gaataagtcc gctttgcgat
    40501 agaacatagt caaaggaaat atatgagggc ccgtaaatgt acccgctgca aaggtgaggt
    40561 gaaacagaag gattgtcctc gtacaaaccc cgcatcagtt ttacaagctt tccgttcttg
    40621 caatcacgtt gtattttatt atatgcattg gaataattgc tgtacttttc caaaagcatg
    40681 tttgttgtat aaatcatatt ctcctccaac aactcccagt atagagtagt tattggagaa
    40741 aatcaagagt aaaatgagta ttctttcatt ctttcattaa gaattttcca ttttccgccc
    40801 gatttgttaa aaaatcaaag ttataagggt ctgttttacc ggatctgatt gtaaagcagg
    40861 gaagtctcag gtcaaaatta gtaacagcac tgcctgctct ttcatataac ttactataga
    40921 tttgatgcca aagaactgac ctgctccacc cgtgctcaat tgttttattg acataatata
    40981 atgcctcttc aacagtgttg catttgttga taattctctg attatgaccc tatggtatct
    41041 gtatcacgag atttgcaact ttttcttccg tttcgcctac aacttgtagg ccttttctaa
    41101 aaaacaaata ccactgtctc atgtatttaa tatttgtttc agaaaatcct ttcatttcgg
    41161 ggaattcttt ctgtaaatct ttacttaaat tagggataaa agcatctcga catgagtgct
    41221 tttcctgcat ttaaacgatt tcctttccga aactccaata caagtccaag agttcataat
    41281 ttaccttgac agccacttta aactgactga ttcttgatct ttcttttagt tccgactgta
    41341 attttttata ttcgttgtca tttataacgg acaattcatt gttattcaaa tatagaagta
    41401 tcctaacata tatcattgtc taattatgcc aattcatgcc acgcttttaa tcaatattgt
    41461 ttgttgttcg tttacggaaa catattcacg gaattttgtc tgtcggcgga acaatttcaa
    41521 cctataataa atcactcttt cctaaattgc aatttgagca aagggtctgt aaattattca
    41581 ttacagtttc tccacccttt gaccacggga caatatggtc aacatgcaat tttactgatg
    41641 ggtcttttgc cggggacgca ccgcaagtgc aacacttaaa attatcacgt tgtaatactt
    41701 tgaatctcaa acgaagattg atgtctcggc ttgttttacg acgcggtgta atatcttctt
    41761 tcacattttc taataaattc tgttcttttt tgccattatc gatttctgtt gtttcttctt
    41821 ggatagcctt ctcgtaatcc tcatcattat ttatatactc cacaaaagct aacagtgcgg
    41881 ctctccaccc accaaatctt cggatataag tatttaagga atactcggat aacccattct
    41941 ttacatctgt tgtagtaggc tgtttacctt ttttgctcca cattctttct atttcatcaa
    42001 ataaatcttt attgcttatt atcttaaaat cagtttgttc aagattagct ttttttagtg
    42061 cttcagacca tgtcgaaaat ctagaaagaa ttgtttgtac cttaaatttt ccatgttcac
    42121 tgtaatcttt tgtagaaata gacttagtat ttaatcgttt tgataccaat tgcatgtcat
    42181 tcaataaatc ttcatctgat atacgtaaaa agtcttcttt attcctaatc gttttaagcc
    42241 cagcaatatc gcaagcagcc aaccacgaac caaagttcct aatatatgga gttgcagagt
    42301 attttccatt tttctcatat acgcttcgag aaagatagct tttattcagt ttttcggcca
    42361 ccatgcgcaa atcttttata agctcatctt tgcttatatt tctatgatac tgatttaact
    42421 caaatttcat aaattcacct acttttatct cgttatctat tttatcttct caacatatca
    42481 atttcatcta tcctatccgc acaacactaa tagctattta gatcattcca aaatgttgaa
    42541 atgctttttt tatatctctt agcttatttt ctggacatgg atgctttcgc ttgttcaaat
    42601 ctattctgtt gtttgcagtt ttaggtgata aaccataaat ctcttttata tgcgcaatcc
    42661 aacatgtttt tggaagatat ccgaagttta ttgatacata ttcttgtatt tctttataag
    42721 ttgccatatt caattctcct tttatttact atagttatca agtaatactt cagatgatat
    42781 cttgggaatg ctcacatcaa tcttgaattt aagtatatcg tgctttttac agctttgcaa
    42841 cttttctctt tctaagccag tctttaattt agccttttgt cattaaatat tccggatgag
    42901 caacaagttc gtctattcgt gatgcattaa tgtggagtat tccagaattc ctgttatttc
    42961 cggcagaagt aatatatgta accaaaactc tataaccatc tgccagctgt atatattcaa
    43021 ttagttgatt tttaacataa tcatactgtg gtctattttt atagctgttt tctgttaaga
    43081 atgaggatct ccccttaaac atagtgaggt actaaacatc aaaactaccg tctcaaccct
    43141 tatggctccg gaatctgtac tttccttttc caagtcccgt gaccggctca atgaatcctt
    43201 tctccgtcat ctcccccagc agtgcagtac ttctggtagg tttcaggccg agagctttct
    43261 gaacatccga tctgccaaag atagtctgaa agccaaatgt ttccagcaat ttgtaaacat
    43321 gggaagccgt ttttgcggtg aatgaacttt caatgttcac tttttccttt tcaatgttcg
    43381 ctttttgggc tccaatgttc actttttccg gttctataaa agcgttgctg atatgcaaag
    43441 tccggttatg gagcggatga ttctcgttta aaagaagatt tcgcaagaat agctccaaaa
    43501 attccgtggt ctcgtgtatg ccgtttttca gatcgttata atttgcccga acaagggagt
    43561 tgcggaaata ccatgcattc tcagcaaaaa tatcgtttgt tacattaaag ccaagtgtgc
    43621 gcagatactt aataaagaat actgcagtcg ttcttgtatt gccttctcca aatacatgaa
    43681 tctgccaaag tctggatata aacacggcaa ggtgatgtat aatctcgtcc atcgtgaggt
    43741 ttttatagga gaactttttt tcttcagaaa agtcgtattc taaagtggct cttaggtcag
    43801 tggcgctgcc gtaaagcacc gtcgcaccgt caagcaccca ttctttcttg gtaatgttat
    43861 aatcacgaat gcgacctgcg tgtggatgga taccggcgaa cagctttctg tgtatggaaa
    43921 tatactcact cggagtaaag ctgaaggctt tttctgaaag aagcgccgct attctcaccg
    43981 aaaccttgtc tgcttcttcg gtacgatttt ccacgttacg ggcaggattc tcctcataat
    44041 aggtctgcaa gagcgcattt gcctcttcaa aggaaatctc gccttcaata ttacgaacag
    44101 cggtgtgcac caagtattcc gatgtcttta gtccatcaac agcctgaaga ccaatggctg
    44161 tataccatgc atagctttta tcacgcttgc ccggttcagt ttctttgata tattctttaa
    44221 aaggatcgtt attcaccggc taccacctcc atttttttac ttttcgctat ctatagcttt
    44281 tatcccttct tctatttcct ctatagttgg caggctgctc ttatattcct tacccaagac
    44341 cttcgaattt catacttgct tattccaatg ggttgtgaag tgcttttgag agaatattct
    44401 gcagggataa aagcatccct aacccgtaac attgtctaat tataccaatt tatgctacgc
    44461 tttgctatcg ttgattttta catgaaatgg aaaaatcggt tatagacaat aaacataccg
    44521 tctccacatg gctcgagttt atggaattga tgtatgtggt ctatttaggc aaccatctgt
    44581 ttacaggaac atatcaatca atttgtcggc gatttttgat aaatagctgt tcaaggaaac
    44641 atatcaatgt tgttatggct ataagaaatt atctttctaa cgtaatgatg gattacttat
    44701 tatacaaata agagtaaagc cataatagta tgatcaatat tcattctatc tcggcttctc
    44761 caatcatccg ataatttctg acatgaaaaa tcttttccaa aattccaaaa accttcttta
    44821 tttcttcttt ttatcagaag gtccagttct tcttcaaatt cttttttaaa acatgaaaat
    44881 ttggagagaa gaaaatatac cttatacagt tcagaaaact tattattaaa gccttgactt
    44941 aatataacac ttgtgtaacc tatcattttt ccattatgat gcagccattt taagtaattt
    45001 tgttttaact catcacttat ttctgcttgc atgttgccga ataactcaat gagataaatt
    45061 gaattgagtc caatataact tccatcaata tcacatccca ataattcttt tgatattttg
    45121 tttgttcttt ccttgtcata ttcaccatct gcaaatgctt ctcttaaaat agtatgccag
    45181 caattgaata cttctaaata ctccttacaa tttgaaccaa atattgagag tttagaagct
    45241 acaaatagag gttgagcagc cctgtaccat ctatttcttt caaaaccatc aggccattca
    45301 atttcatttt tcaatatctt ttccatataa tcacatagtt tatctactaa taaatttcca
    45361 cgttggatat ctaacatttt tattgaatca gtagcccgtt ctgtagtagg aaatttctgc
    45421 tttattttag aattttgtga atgaaacctt ccatatccgc cgtcattaag ttggtttgtt
    45481 ttcaataact caacccactt gctgttatag aattcatcaa acagttccga gctaatttct
    45541 tgatttaata ttctctttat tctgtatcta actgcaacac aaggagataa ctcttgaatt
    45601 cgatcgatta ctttaaatac gttcataccg tacttccttt ccagagttag ttatcggtca
    45661 acgggttact ttatcatttt acttcttgag ctatttttct tgcgaaccat tttgcacctt
    45721 ccacagcctc tatagtctgc atagaagcct tattgtctgt atctcttaca ctatacatgc
    45781 caatatattc tagggacgaa tgcacgcaaa gattctttac tcccgtttca aaaggggtac
    45841 aggcgtattc cctgtcgtac ccgtgggtaa ggagaagagc tatcttcttt ccagcccaga
    45901 gggaacctgt ggcacttccg taaaacttat tgaggccgta atgtctgtct agtacggatt
    45961 tcataagcgt tggacagtac catgaataga tgggacatgc taatacaatc acatcggccc
    46021 acaggatatc ctctattatg gggtacatat cgtcatgctg gatgcatcca tattcatcct
    46081 gtacatcctg acatgcgtaa cagccgaggc atggacgaat atcctttcca gctaatatga
    46141 cgtacctggt gtcagcccca atcattttta gttcctctag aaaaggtttt acacaattct
    46201 gcagtatttc cgttaacacg gggactaccc attaaaatga gtacgttcat ttatcttcct
    46261 cctttcatga agttttcaat ataaaaatat cccaacttca ctaattcaga ctataattcc
    46321 aattcactta aattcagcgt tgttccttat aagtagtttc ttttccaaga agaacttgga
    46381 tctgtttatt cttctctgac cattctttgc tcattgtgct ttttaacctc cttaattttc
    46441 tctcaccaac tgaattatta tatttttagg gatcgttcca taggttcaga aaaatcttga
    46501 acttcatatt tctaccaata ttataacaga tctaattcaa gagaataaat gccatttttc
    46561 tgtttcgtcg atttatgagt atgaggttcc attaacatgt atctaccact gttcaaccta
    46621 ttaatcgtct ttactgagca cacttgtggt gtatgagttc aaccaagata ttgcttctta
    46681 tcaccaaagc aatatgtctc tgcaccggaa ttgcctaaat ataatttgca atcttggtgc
    46741 agggataaat acaattcatc atgcagttca gcattaccat gaactgttat tccaaaaata
    46801 ttatagccac agttgagata gctctttata accgcttctt tatctctccg gtacattgga
    46861 ggatgacata ttttcatttt gaaaatccgg ttcaaacata aagaactttt ctttgataaa
    46921 agtggtcatt aatacgccat tgctttttat cactctggcg catacttccc caaatggctg
    46981 aagatgttcg atatagcagt tcgataaaga attaaaaatt aggtcaaatg agttagttta
    47041 atcagataat aatagaacaa aacaagaata taatgaatca tattaatatg ctaattttac
    47101 cttattctac tgctttacgc aaccatatgg caaatatggt ctaatgtatt attacccaag
    47161 aaggaaataa tagtgtttct agggtctatt caaggcatac ctgtaaccgg gatagaaact
    47221 atagttcaaa atttaccgga gcttttcagg gtcttaaaca agaaatcctt aaaacttcag
    47281 agtctgggag agaccatagg gtatttctgc aaacaatatt tggtttccat tgccaagttt
    47341 gcatatactt ttgcagtaaa tgggatgact attgatgctg ttattaccaa attagggata
    47401 gttgcataaa cttttacatt ttactgcaca acacaaatat ataatattgt aatatataaa
    47461 atcataatga tataattata tatagggagt agtatcctta catataagtt tataaaaatg
    47521 gggggattat catgaagtac tatgtggatg taaatgctgt gaaggacggc gatggttcca
    47581 aggagaggcc attcaaacgc ctcaacgaag cagcgaagat tgcccgtccc ggcgatgaag
    47641 tgttagtagc accagggatt tatagggaat atgtagatcc ggttcactct ggtactgaag
    47701 acgcccgtat cacttatatt agtactacac ctcttggagc tgtaattacc ggagctgaac
    47761 aggtaaaaaa ctggcagcat tacaagggaa atgtctggat gtgccgaatc ccaaacagtt
    47821 tattcggtga ttacaatccc tatactactt tagtatatgg agactggtat tttgctacac
    47881 caaacaaaca taccggctgt gttttcttaa atgacaaggc gatgtatgaa gccacaactt
    47941 tagaagagtg tattaaaggc gaagtttatg agtgcagctg ggtgccagaa gaatccatct
    48001 ataagtggta tgccgaacag gacccagata ctgatgaaac gatcatttat gccaatttcc
    48061 agggcctgaa tccaaatgag gaaaatgtag agattactgt acgtcgtagg tgctttatgc
    48121 catctaaaac aggcgtcagt tatattacga tcagaggatt cagaatcgat aaagctgcca
    48181 ccacctgggc accgcctgca gcttttcagg acggcatgat tggtcctcac tggagcaaag
    48241 gctggatcat agaagactgc gagatttcaa acagcaagtg tgccggcatt tctctgggaa
    48301 aatatcttga tcccgacaac gagcattatt tcacaaataa gcatgtaaaa agtcctaccc
    48361 aaatggaacg cgatgcggtt tgccgcggcc aatatcacgg ttggctgaaa gaaaatatcg
    48421 gtagtcatat aattcgccgc tgtaacatcc accactgtga acagggtggc attattggcc
    48481 gaatgggcag tgtgtttagt atcattgaag acaaccatat ccaccatata aataatatgc
    48541 aggagcttgg tggagcagaa atttccggaa taaaattgca tgcggccatt gatgttatca
    48601 tacgccgcaa tcatatccac cattgcacaa tgggaatctg gtgcgactgg gaagctcagg
    48661 gcacacgtat tacccagaac ctacttcatg ataaccagcg gccgccgtat gccaaaaagc
    48721 ttgaaggcgg tatgaattct caggatatat ttgtagaagt cagccatggt ccaacattaa
    48781 ttgataacaa cattctgctc tcagacgtca gccttcgctt tgcaacacaa ggcgtggctt
    48841 tagttcacaa tcttatctgt ggtgcattta cctccgtcgg cggtggaacc acttggcgtt
    48901 atacaccata tcatatacca caccgtacag aagtgatggg ctttatgacc attcttcacg
    48961 gtgacaaccg tttctacaac aatatattta tccagaagtg gccatctgaa gactatgtga
    49021 tccataatga tcatgatcca caacgaactt atcgggaaaa cagacttgtc ggcactcatg
    49081 ttttcgatga gtatccaaac tatgatgaat ggatttcgca gttcgatttt tccaagcgtc
    49141 cagacatgag agccttagag cctgtccatt ttggtcatct gccaatctgg agtgaaggca
    49201 atgtatatct aaatggtgct aaacagtgga aaaaagatgt aaactactta ttggttgaaa
    49261 ataatgatca ggatcttaag gttgaattag tagaaaaaga cggtcaatac tacctgtgca
    49321 caaacatatt tgattacttc aaggatttca acgttcggat gatcaattcc gaagtactcg
    49381 gtaaagcatt cgaacctgaa caatactatg agaacccgga tggaacaccg atacgctttg
    49441 acactgacta ctttggaaat catcgcggcg ttcaaattat cccgggtccg tttgcatcac
    49501 cttctgatga aattaaacta taatcacaaa tataagcatt atatgtaata aacaatggac
    49561 gtctcatttc tggcatatta atcaaaactt tattaatgcc agacggagac gtcctttctt
    49621 ttcatgcgtt cctcaacacc acttacctca tgtacataaa cgatcaacaa caggacgact
    49681 tgtaaatcaa aaggctcact tttccgacat caatgtcaag gtttcaatat aggttaaggc
    49741 tgcagcattt agtacttttt ttgctccttt agattcatct tctgcaagaa aaccactggt
    49801 atcaagcagg tttaaagctc tgctgcttgc attaaattct actggaagtg taataatttg
    49861 aaataaaact gccattccaa aaagtattat tcctatattc ataaggctac ctaaactagg
    49921 aataagtaat cctacaatca taaacggcca tgctaaagaa gaaccaattc tagctactgg
    49981 aaaaacctta ttccttaaag ataatgggat gtatccatta gcatgctgaa tagcatgtcc
    50041 tacctcatga gcagccacac taactgaagc tatggaatat ccttggtata cttcttgtga
    50101 tagccttaaa actcgttttg ttggatcata atgatcactt agcttaccat gggaaacctc
    50161 aattggtata ttttgcaaac catgttgatc tagtaagttt cttgctactt gaactccagt
    50221 atatcctctt tttgttggga ttctcaaata tttagcaaaa cttgattgca ctttaccttg
    50281 agcatatata gttaagataa ctgctggaat taaaataatc attgttggat caaatctaaa
    50341 ataaccaaaa ggataaaaca ttattatcac tcctcaattt aaattttaaa aaattgtttt
    50401 atgaacaaat cttaaaccca taccttaaat tatctaggct tctaattctc ttaatacagt
    50461 tttgaattgg atagcaaaca ttataccggt tattgtaaca gcaataatat cagctactgg
    50521 ttctgctaaa aatactgcat tgacctta
//
LOCUS       DAOSOM010000012        46986 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_824_length_46986_cov_5.770599, whole genome shotgun sequence.
ACCESSION   DAOSOM010000012 DAOSOM010000000
VERSION     DAOSOM010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 46986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46986
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_824_length_46986_cov_5.770599"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="PLF14_04410"
     CDS             complement(<1..449)
                     /locus_tag="PLF14_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018110062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HOT70573.1"
                     /translation="MIKTVVIGYGNIGIAVLEALSQSHDFAVAGVVETNMDLSKIKID
                     YPIVDNIDKLSGFDVAILCTPSRVVPMVAESLLNKGIYTVDAYDIHSGICDLRRKLDK
                     VAKKNNVASIISAGWDPGTDSIVRALMEVMAPKGITYTDFGPGMSMG"
     gene            complement(592..>6848)
                     /locus_tag="PLF14_04415"
     CDS             complement(592..>6848)
                     /locus_tag="PLF14_04415"
                     /inference="COORDINATES: protein motif:HMM:NF020018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HOT70574.1"
                     /translation="VATSQDDVKAAVKVPNYPGGQAQPVITVDQGQALPDGNTSGDYP
                     INVTVTYPDGSTNKDVVTVSVYYQYTINNLIRLENGNNTAKPVPDKPQEIGSLPLGAH
                     TFQLQAIGKYAPAANQITDVTIQAEGNVFTFYYIDEAPVFTEFEDIYVGYNDAFDPKT
                     GIKAIDDEEGEIDYTVTPAAIDTSVPKEYTLTYRAEDSSGNETVKTRKITVLPQPVYD
                     YTIYFLIRLENGNNTDKHVPDKLPKTGQLPEGPHSFGQLQPIGKYAPVPNQVTDVTIH
                     TEGNVFTYYYIDEAPVFTGCENTVIEYGTPFDSKAGVTATDDEEGEVEYTVTNEVNVN
                     RPDDYILTYRAEDSSGNVTVKTRTVTVLPRAPYNLTSINNRQTHPTTTVITGKADPGA
                     VISVEIAPKTWIVQVGAQGNFTLSIDKQLKDTVIAVKATLNGHVKSTNVVVKNVQADD
                     YLPTITNIVKQYGVPTTEAEVINAVTVPDYPAGPQQPQVTVNPNQTIPNGNTSGDYLI
                     NVTVTYPDGSANAATVKVSVLYRYTVNYRIRLENGNNTNKQVPGINDITGSLPLGPHD
                     FGQLQAVGQYAPAANQNTVKTIQTSGNNFTFYYVDDAPVFNGCDDSIIEYGTPFNPRA
                     GVTATDDEEGEIGYTVTSQVNTTRPGNYILTYRAEDTSGNVTVKNRTITVLLRAPYDL
                     NAINDSKAHAATTVITGKADPGAKISVTKDAKTWETVVNAQGNFTLKVDKQNKGTVIE
                     VKATLNGYSKTADVIVKNVLADDYEPTVNDIFKKFGVATTEAEVKAAVAVDNYPPGQL
                     QPALTVNLAELPDGNTSGDNEVSVTVTYPDGTKDDVKVTVKVNYRYRVNYFIRLANGD
                     NTTKPVPNILPKTGDLPVGQHDVGPLPDGGAYDKVANQDTLVTIDGTTDNVFNFYYVD
                     DAPVFNDFDDVTLMYGALINPKAGVTATDDEDGEIDFTVTPKVDTTVPGTYTVTYRAE
                     DSGGNVTEKTRKVTVSQAQNVTYEPVVVPEIVEYNGVVNLKDNVTNIQQLPEGTMILD
                     TTHPAIDTTVPGKHTGQITVIYRDGTSDVFNVDVTVQPQPMNQIYEPNVFPEIVEYGE
                     VINLLDNVANADTFPDNTDISDTTDPVIDTTVPGVYPGQITVTYPDGTKDVVNVKVTV
                     NHEPDIPDNQVYNPNVVDETVEYDGVIDLTDNVLNFENLPDGTQVTDTTAQPIDTKVP
                     GTYQGQITITYPDQTTDVYNIQVTVKPQPHNEKYVPVVVPETVDYGGVINLKDNVTNA
                     DTFPDNTDISDTTAPVINTMVPGLYRGEITVTYPDGTKNVVNVQVTVNHQPDIPDNQS
                     YNPVAVDETVAYGGVIDLTDNVTNLDNLPDQTTVTDTTAPPIDTRLPGTYQGQITITY
                     PDQTTTVINVQVTVLPQPLNEKFEPVVVPEIVEYGGVINLKDNVTNADELPLNTVIAD
                     TTVPPIDTRSPGVYQGQITVTYPDQTTDVVNVQVRVQPEPDYLADDEANAPVVIDEIV
                     AYGGVIDLTDNVLNLKDLPAGTRVMEIYKQEIDTTVPGTYRGQLAVIYPDASVIVSDI
                     RIIVKERTQSDTHNPVVRDDETVGYGEDIDWKDVIENIGDLPPGTTVTETSENPIDTT
                     VPGTHQGQVTITYPDGSQEVIDVTVTVEEQPQSDTHNPVVRDDETVGYGEDIDWKDVI
                     DNIDDLPPGTTVTETSENPIDTTVPGTHQGQVTITYPDGSQEVIDVTVTVEEQPQSDV
                     HNPVVRDDETVGYGEDIDWKDVIDNIDDLPPGTTVTETSENPIDTTVPGTHQGQITVT
                     YPDGSREVIDVTVTVEEQPQSDRYNPVADREPVGYGEEIDWKDVIKNLEELPAGTTVT
                     DTSEQPLDTTVPGTYTRQITVTYPDGSEEVIDVTVTVEEQQQSDRYNPVVGSETVEYG
                     KEIDWKNVVENLEDLPEGTTVTDTSAQPLDTTVPGTYTREITVTYPDGSTEVMEVEVT
                     VKEDSGQTPPPTPGPDTEEETPEEQKEESWSLVSVISTAFSALAMLIQLLIKSVRVDD
                     SGTHFVRNKIFKLITAVLAVVAIVYLFSTNNLSLTMRMVNETTPVSAIMTVATTTSLV
                     FGMRWKPGKK"
     assembly_gap    6849..6948
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6949..14563)
                     /locus_tag="PLF14_04420"
     CDS             complement(<6949..14563)
                     /locus_tag="PLF14_04420"
                     /inference="COORDINATES: protein motif:HMM:NF020018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HOT70575.1"
                     /translation="MVILLIAILALAGMPGKVQSQGSYDTNFSWTTDEEVLNEWPLDA
                     TIQSTSYTGIGTAGFSYHHYYYDEQDRIVLEFELSMFPASAGREVSASRLFRENWQNA
                     HIFIDPKLINKVDVDASFFQMYYPKDPTTVSFNLGDNTGTENVYKFTIDQVYPQKPAN
                     SDYMKSKLYLILKDTVTREQLNDDYALELRYTNTGNQVYNQKGANGRIVLGNYLNYFE
                     ELDNYDPSADNDDVTLKTIAPFHTASMSNLVANPYLPPDMMRTVAQSVIYDSAQGKLH
                     VYYRVAPNHYTYRTFANNGLFLSSWIGIRQVMDSRIYDALLPDADGVVGQMRMTDLDG
                     VPGTNWSVSTEIRADEFSYIPTTPTVGTYSYMMVPAGFKTDVVNKAVVPADQTTNIKN
                     VYLHGYRKQSDFVRFIYNVDKNKMDALFAQGNYSTLSISTAYITDRPTEKTQTEYRLT
                     PTQNIVIPKGARLVFELPETSRSVFKSGPNNNYERLIGNLKTKVDGQMSTGVDPNDYG
                     EGYTQTFYSGLIFGGHVLTPETGLTLRAGETVNLTVFDTASPNQVKMTVVTGMYDENR
                     VEYILTKHAENENKIYNLAHSNVRTGIIINRSANTPHVDEFFTDSASITGHSKYPLAL
                     VSVRKASDGAVFREANSSSILQDFTAEGSNVQGYAFTLDIPADVSLKKDMALSFSNSA
                     IGFMRSKASTYRAQAKVTFDKNDGTGNTVVRIVPINQKAYDDPGYSPNGFDGPNILKV
                     SQGTIEYLSDYEGKALTDLGDIEDRTFCSDPTRAGYNFLGWSTKQVDSMGATTFADLP
                     ALTDVAQWESTDNYKFTATSPVDQGRTVYAVWEKNTIEQYKIVLHNNNGGDMNVSHII
                     YVPVADITGDNTAELTTYLGKPGNILYDNDFQKADCYFVGWAEQAVVPDGTLVHQLYT
                     NASKIRLNGGKLQLQLNELPQNPVVHDNPWKDVSADLTNDNGIATLNLYAQYKPLIVM
                     TAEKLWYPSGAKAAYEKWIEDPNNNQKPDPDAVAHFGNSQVAMVLMRTTEGKTLDPTK
                     YEIIPGFYRTTEDGVNWQWAPQEGHDANGRKYSYLMTEFISPEQEGFHTEEEIIDHFN
                     NKRTWSSMYITMLNKGDNLSKYTAITLKDQTNKTHSYLAVTNSNQPDAVQTVKVTENY
                     HFTLRNFKVDLALPLIHRIQTNHTEIKIDSPKDGAKYLCLKLPDYTENPIWFSKGDTI
                     WTSGDDNFTISEVNDELIITIPLTYDFSDKAGKKVYALYTVNNTDIPPDSDCAFSEVM
                     SYDYLPPITDLKQEPHIKDDNGLITHNVVTAKIPAGTYNGAKYTLGYMQGGTFQAVSG
                     NESPDLDEKITFKVPAGTLNEGTTYCIQGTDPALTFKDTTFGPPTVTMDLTGPDITAP
                     DSFGVLTGNLIGAAEGAVTTGDAKDVITFKYTKGGATVSLPDGIAFDYAAGKFSGKTA
                     DVLDVSQTGIYTIAVTAEDVFGNVSTKNIVFTVTQKPTTDAITSITQNANNADGDVSL
                     TVQGTKNGILRFYSEESPTVYTEIVIPSITGSVITNADGTKDIIVPKEEIIRFKDKKI
                     FITQQKANELESDKTDSAGETIDKKNKKKISTGGAVVIDNVAPTPLCVDQPLANTNTL
                     RISNTSVEAVLPDIKDVDKIKLKIGAFIECEPKREYNNETGEPTNIWICGGGYTFTES
                     EEKVWVVLDPNDPDNKVEKTVGVLTYEFTGAKKFEEFQVIEVVYYDYLGNASVPVTTT
                     VPKLPDPVAPSNMTAINNSQKHENTTVIKGKADPGADISVKIGETTWLVTADEHGDFT
                     LEIAKQDKGTVIPVKSTLNTYSAIANVTVQNVMADDYPLTITNINKKYGVSTTEEEIK
                     AAVTIPTYPAGQEPPVVTIDSGQTLPDSNTSGDYMIKVTVTYQDGSTNKGSVKVSVYY
                     QYTVNYCIRLANGDNTVKPVPGLEAKIDSLPLGNRDVGPLPKGGKYAVVPGQDTVKTI
                     KEQDNVFTFYYIDDAPVFVGCGNVSIEHNAVFDPVAGVTVSDDEDISINVFTVQPESI
                     DTSKPGQYTLTYRVTDSGGNETVKQRVVTVKPQPPYDLTAINDSKAHSAKTAVRGKAD
                     PGATVSVTISSSQWSMPVGESGEFTLMITRQPAGSVIEVKSTMHGYTKSADVTVKNVQ
                     ADDYEPTVTDVIKDYGTATSEDEVKAAVTVPGFPQGQQQPTVAVNEAQTLPDGNTSGD
                     YTINVTVTYPDNTTDAATVTIKVKYLYTVKYRRQLEDGTKTDIAVPGKNDITGSLLLG
                     EHSFEALKTEGQFAPVEDQDTDVIIQTEGNNFTYYYKDDAPVFNGCDNVAIEYGSDFD
                     LRTGVTVTDDEDGDLTDSFTVTPDSINTSNPVVYNLEYRVIDSGGNVVTKQRTVTIKP
                     QAPYDMTAINDRQAHQDKTRITGKADKGATISVTIEGTTWFVDADANGDFILNIDKRN
                     KGEVITVTSTLNGQTTSADVTVQNVLADDYLPTVADIDREYGVATSEAEIKAAVTVPD
                     YPAGQDQPLITVDEGQIMPDGNTSGDHPVNVTVTYSDGSTNKVVVTVSVYYKYTVKYR
                     "
     gene            14943..15377
                     /locus_tag="PLF14_04425"
     CDS             14943..15377
                     /locus_tag="PLF14_04425"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOT70576.1"
                     /translation="MASNDKIQKIIRIIEDIELEKVFKSLNDTMTGIGAALHILYEYD
                     RSTTSGQLCDLLGVSTARVAVLLKTMESKGLIIKRKGVLDARTTVVSLTAFGKETTLK
                     IREEMYNRINRIIDHVGFAQLEQFFAIAKQIEKAVTPTANIF"
     gene            15457..18441
                     /locus_tag="PLF14_04430"
     CDS             15457..18441
                     /locus_tag="PLF14_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006862035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HOT70577.1"
                     /translation="MLELKNITKTYHTVGEDVEALKGIDLTFRDNEFVCILGPSGCGK
                     TTMLNIIGGLDQYTTGNLLINGRSTKEYKDRDWDAYRNYSIGFVFQSYNLIPHQTVLS
                     NVELALTLSGVSKKERIKRAKEALERVGLADQIRKKPAQLSGGQMQRVAIARAIVNNP
                     DIILADEPTGALDTETGIQVLDFLKEIAKDRLVVMVTHNSYLAEKYSTRIVKMLDGKI
                     IDDSMPLDIEEKEKITVETESSNKKSLRKKKPSMSFGTSFGLSLKNLIMKKGRTALTS
                     FAGSIGIIGIALIYAVSQGTTAFIDSVQEETLSSYPLTIEAQSVDVSTLLSTFMGKAQ
                     SVSEHEDDAIYQKAMLYEMTNAMNSMETQKNDLKSFKEFIEKERADEENRLHTALSGI
                     QYTYEADLLIYTKNVDGNIIRSDTETMTLELMKKYAGVDMSSMLAMRYQTAMRNSSSM
                     TLWKELLSGDNGRPVHEIIEKQNDLIYGSWPARYDEIVLFVDENNEIDDVTLYALGLK
                     SEEEMEKLMEAAVNKTIIDYEIKKWSYEEICNMDFRTILSSDCYTYDEKSGTYIDLRD
                     SEAGLKYLYENALKLHVVGIAKPGKDAVSSSSRSYIGYTGKLTGYIIEQAAESDAVKA
                     QKEKSNTDIFTGLPFKDKEGMVSAEKKARDFKEYISGLDVSGKATAYIKIMSIPSEEA
                     TQQFVSDTLSGMNRIEMEATVAQAMAEQMDVDRSTVTGYISSMSDEDLQELFTKKLKE
                     QFHAQYAAQVSQQMSAMTSEQLAASLDMAINRYTDEMCAQYYDEIPEFSDSTYDNNLI
                     KLGCVNLDSPSSINLYVSSFADKEIIEEAIAEYNQNVDDLKEITYTDYVGLLMSSITT
                     IINAITYVLIAFVSVSLVVSSIMIGVITLISVQERTKEIGILRAIGASKRNVSGMFNA
                     ETVIIGFTSGVLGVVLTYLLCIPVNLLLHHLTGLNNLNAILPLPTAIALIVISIFLTL
                     VAGIIPSRSAAKKDPVVALRTE"
     gene            complement(18470..18907)
                     /locus_tag="PLF14_04435"
     CDS             complement(18470..18907)
                     /locus_tag="PLF14_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015525548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOT70578.1"
                     /translation="MIYTVFTNKALRLAYDAHHGQTDKSGQPYIFHPYHVAEQMTDEI
                     SVCVALLHDVIEDTSVTFEELEKEFPREVIEALRFLTRKSGTGYLDYLREIKQNPVAL
                     KVKLADIAHNSDESRFEGCNELSAEDIARNKEKYAKARAVLEE"
     gene            19111..20199
                     /locus_tag="PLF14_04440"
     CDS             19111..20199
                     /locus_tag="PLF14_04440"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOT70579.1"
                     /translation="MKKRTAVKIVASLLLVAMIGAFMVGCGTDNDKVTASPKTTKSVS
                     STSSPEPTKNTNPIVVTWYPNESSDTHEAVRNEVGRLIEQATGRKVTHKLTTDYTITI
                     EAIASGSADIAIAMGAVGYIEAKNRNPEIDVLFVNSDENWSLDGAKYFAWICVPAEKA
                     DQYKSGDTYSIDNIKGKKMSFVSNSSTSGFRVPTSSIISHFKSDNLNEDKLIEGGPFF
                     KEVYFGGSHQGSAVNLLSGNADVAAFCDIEVQPYVEMKSGDISKVGSVVVVKKGADAP
                     FDQYVGRELTIIAVTPVFNGPNVYNPKNLSKEEIKAIQDLFCSEEVASNKILFYDDKV
                     EGAKGLYKKSKSYGFVLTTDSWYDPYRR"
     gene            20220..20990
                     /gene="phnC"
                     /locus_tag="PLF14_04445"
     CDS             20220..20990
                     /gene="phnC"
                     /locus_tag="PLF14_04445"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HOT70580.1"
                     /translation="MPILEFKNVSKVYNNTTKALTDVSFSVDEGEFVSIIGPSGSGKS
                     TILRCINRLVDVTQGSIFFDGHDISQADKKEIREIRKKAGMIFQHYNLVNRLSVIENV
                     LHGRLGYKSSIVGAVGHYTEEEKERAFHILAMLGLTEQAYKRCDELSGGQKQRVGIAR
                     SLMQEPRVLLCDEPIASLDPSSAKVIMDYLEYINETMKITCIANLHQVDVAMAYAKRI
                     IGITGGQILYDGPPEKLTKAQIYEIYQSDEGELITDAK"
     gene            20980..21843
                     /gene="phnE"
                     /locus_tag="PLF14_04450"
     CDS             20980..21843
                     /gene="phnE"
                     /locus_tag="PLF14_04450"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010676731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HOT70581.1"
                     /translation="MQSKQVNKKVYNDKTENNAERAPRPEKQTSIFVKRKRSFTLVFL
                     GIIALFAVSALITEYNVITGFESIPKAVVWMAKQFIPDAKAWSRLSSILKKLAETALV
                     SVAVTVCAAICAFFLSLFGTKTTKAHPLVARIVRIIAAFFRNVPDVVWAMLLLFSFGQ
                     NILTGFFALFFATFGMLTRAFIETIDEVSASCVEALYATGATSLQTVFNGVIPSSMPE
                     IVSWVLYMIETNIRSSTLIGMLTATGIGYLFDLYYKRMDYGSASLVVIAIVILVIAIE
                     IISNRIRKVIM"
     gene            21843..22712
                     /locus_tag="PLF14_04455"
     CDS             21843..22712
                     /locus_tag="PLF14_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015616602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOT70582.1"
                     /translation="MSELNMTEKLPVYIDIKEYMRKTRSGKIKTTAKSKGGIAIRITI
                     FALVALTVYGFLTFDYKDIDMAKAVAGTWNNAKVLFGQPKLTYNTLAGALKELLVTFS
                     LGALSTIFGAIIAFFGALLCAKNIANPIVSGITKSFVAFIRAVPTILWVLIFTVAAGL
                     GSVAAVIGLTFHSCGYLIKAYAESIEEVDAGTIEALKASGASFWQIVFQAMLPSSISR
                     MTAWTFLRFEINFTNAIAVGAAAGAGGIGFDLYMAGSHYFDLRELGFITYIVFAAVIL
                     LEILATGIKAKVK"
     gene            22709..23689
                     /locus_tag="PLF14_04460"
     CDS             22709..23689
                     /locus_tag="PLF14_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003510782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde reductase"
                     /protein_id="HOT70583.1"
                     /translation="MTMQQVFYDFSIFNILKGKKPLLVCDKIFDTFGIKFPCKVMRFD
                     GFSSNPVYEDICTGVEILKKNNCDFIVAVGGGSTIDTAKAIKYYSGADYPLMAVPTTS
                     GTGSEATHFAVVYINGEKHSVSDQCLLPKYVILQSNLLKTLPLYQKKCTMLDALCQAI
                     ESWWSKRATEESIRYSKEAIDLIMKYMDSYLKNEDSGNAKMLLASHLAGKAINITTTT
                     APHAMSYKLTSLYGIPHGHAVAICLPKVWKQMSNFDDIAIALGQQNHEAAISFFEELL
                     QRLNILPPDNVNKEDLDILTNSVNIERLSNNPVQFDKKTIKELYREILEV"
     gene            23690..25531
                     /locus_tag="PLF14_04465"
     CDS             23690..25531
                     /locus_tag="PLF14_04465"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF010006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HOT70584.1"
                     /translation="MDKRVKAVIFDWAGTTIDYGCFAPIKGFIDGFKSIGIDISNEMA
                     RKPMGLLKLDHTRAIAAMLPEPVTEQQILRAYETFEETLFANLEQHCDVKDYVIETVA
                     SLRARGIRIGSTTGYTSEMMNGILHKVSAQGYSPDYWISADKTAKGRPYPYMIWNNLM
                     HFGISDSREAVKVGDTCTDILEGKNANCWTVGIIMGSSELGLTRREVSALSKNELEER
                     KAIVRASYYKAGADYIIDDMNELLDVITDIDRKLNQNAARKLLTPGPLTTRYSVKHAM
                     LTDHCTWDDEYKAITTSVIKDITHISANDDYTTVLLQGSGTYAVETMINTLCRKDEKI
                     LFLVNGEYGKRMLTIADKSGKDYTYLSFDMTQPVNVKAVEKELQKHKQDISVVAFVHC
                     ETTTGIINPVEELTKLCKSYGKKVLVDAMSSFGAYDIDMPSLDIDALASSANKCLEGL
                     PGLAFVVVKKTLLETSSGNSSSHSLDLFDQYKCFCETNGKFRFTSPTNILLALRQAID
                     DYKMEGGLKARKKRYMENHKVLLQELEELGIKSIIAPENQSYIITTFELGNLDFFALY
                     QSLKSKGFVIYPGKLTSRPTFRLGNIGDVYPEDMVALADAIKELYRF"
     gene            25860..26156
                     /locus_tag="PLF14_04470"
     CDS             25860..26156
                     /locus_tag="PLF14_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009662202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOT70585.1"
                     /translation="MQIKYRTKAIGRICTNASVAVKKYGIEMAERIHLRIDQIKAAES
                     VEFMIKYRIGRCHMLRGNRSGQYAVDLVNPNRLVFEITGDEIQIVNIIEIIDYH"
     gene            26177..27229
                     /locus_tag="PLF14_04475"
     CDS             26177..27229
                     /locus_tag="PLF14_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HOT70586.1"
                     /translation="MIKSRTYIAIPPGTTIKEQLTDRKMSQKEFASRMDMSEKHISRL
                     INGEVQLTCDVAMRLESVLGLPAHFWNNLEIIYREKLIKAQEENEMDADLEIVKKFPY
                     NEMAKNGWVEVTYNPKEKVFNLRKFFEVVNLGLVSGHLIPRIVCRKLGDGDKSDYALI
                     SWSQKAKLESRSLSVRSVNIEKLKTLLPDIRKMTTKNPSDFCPILRKELADCGIALTF
                     LPHIGGSFLHGATFYDGGKIVLGMTVRGKDADRFWFSLFHEIGHIVLEHINQTEGTTD
                     EDEKAADAFARNLLIPDEQFDAFTLNKDFSKTAIITFANKMEIDAGIVLGRLQKEQFI
                     PFSRHSELKTKYELTA"
     gene            complement(27312..28721)
                     /locus_tag="PLF14_04480"
     CDS             complement(27312..28721)
                     /locus_tag="PLF14_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019239060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT70587.1"
                     /translation="MKRFINRYAEIEELEGEYNRKGSSFVVIYGRRRVGKTTLISNFI
                     KDKNALFFLASEESEWQNMLIFQKKAAEFTQNELLRDADIKSWDTIFKAIMTADYESK
                     PVIVMDEFQYIGKSNSAFPSVLQRIWEEILKDKEVMLILCGSLVSMIESQVLNYNSPL
                     YGRRTSQIRLTQIPFKHYHEFFPDKERHELIEMYAITGGVPKYIELFSEADDIYSAIK
                     NNVLGTSKFLYEEPQFLLRQEVSEIGSYFTIIRAIAAGKTKISEIASFLEVKATSLTK
                     YLKTLIDLDIIERKIPITEKNPERSKKGLYRIKDNFIRFWFLFVYPNRSYIERGNTEF
                     VMQLIRKNLVTSHVSYVYEDVCREKMWDLSDNGKLPFRVSKVGSWWNSKTEIDIAAIN
                     FDEKKIILGECKFWKDKVGMNILRVLEEKARTAVPESSAYSIYYILFSAAGFTDELKS
                     CAQSRKDLLLFDDLLSNNK"
     gene            29004..29657
                     /locus_tag="PLF14_04485"
     CDS             29004..29657
                     /locus_tag="PLF14_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70588.1"
                     /translation="MKLILEDESISAKNMRNCHKIMLDIFGDDVELVDYWGPVQMCVF
                     YLEYNYSPDDYKIIMECERGFIVIEVQDKAGNVFWPFMIFPQANYFHYADVEKDVLQL
                     VELTHKAIKENLIIFEPSGTTKYSDSTCLHCFDVREVHNFISPERYEETIEYIKELIE
                     KENFILVEGNCPIGEHKKDGYWIDDIIYHVIKCPKCGWKFTCIVNTYRGSGSFTRER"
     gene            complement(29880..30152)
                     /locus_tag="PLF14_04490"
     CDS             complement(29880..30152)
                     /locus_tag="PLF14_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008797335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HOT70589.1"
                     /translation="MLKIKYHSSFKRDYKRLKKRGMNMDLLHEAISLLAKNTPLPEHY
                     RDHALTGNLKGYRDLHITPDWLLIYKIKRKELILILSRTGSHSDLF"
     gene            complement(30139..30411)
                     /locus_tag="PLF14_04495"
     CDS             complement(30139..30411)
                     /locus_tag="PLF14_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HOT70590.1"
                     /translation="MGQTNITIRMDRELKQEAEKLFNELGLNMTTALTMFTKAAVREQ
                     RIPFELSLSNLNSQTLSAIRDVEEGKNLHGPFETIEELVESLNAED"
     gene            complement(30631..30713)
                     /locus_tag="PLF14_04500"
     tRNA            complement(30631..30713)
                     /locus_tag="PLF14_04500"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30677..30679),aa:Leu,seq:tag)
     gene            complement(30749..31345)
                     /locus_tag="PLF14_04505"
     CDS             complement(30749..31345)
                     /locus_tag="PLF14_04505"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HOT70591.1"
                     /translation="MKKYTPVIIITVCFVFLIIFLIVRNINRGFVDIPDEHELSTAST
                     KETIKICLKGEINNPGIYEVEKGSILTDLIDKAGGLTEDAGQDINMVMVLDKNMTVYI
                     RPKSRAGGIDISDNSDVIIQKDDEKVLFENKIDINTSSEETLCLLPGIGSKTAQAIIA
                     YRIKNGNFKVIEDIMKVPGIKNAKFEKIKEYITVSGED"
     gene            31451..32782
                     /locus_tag="PLF14_04510"
     CDS             31451..32782
                     /locus_tag="PLF14_04510"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOT70592.1"
                     /translation="MRKSISIILVLTIAACLFNINVQGNAQSIINPELCRSYISVDYE
                     TGDVLYSYNADKRIYPASTTKLMTALVAVEHCSLDKVFTTSLNATRYLEEGSTELELK
                     ENETMDLYSLLHALLIRSYNDVALVIAENVAGTEEAFIEMMNKKALELGASNTHFVTP
                     HGLHDDAHYTTARDMALIAKAAFSNFNISTICRKKEFNLPATNLRQKFETLKNTNLIL
                     GTNTGYDFVVTAGKTGYTSKAQNLLVALSTDQKGREVLTVMAGTKERETATELTLELM
                     KYSYTDFGVQSIVKAGQIIGTHEDIRLANAMYIEHLVPYDTDSWQLDKTIELRDDIIY
                     TQVRVGDILGEVSYSYNNNYIGKSLIVAADSLIPEVTSVKISSEDPPKDVAVTAMVER
                     QPFPPAFIAALSISVILLVLIIVVFYKTSKRTVDKTKEKEDNNDKDTKDNG"
     gene            32797..33384
                     /locus_tag="PLF14_04515"
     CDS             32797..33384
                     /locus_tag="PLF14_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70593.1"
                     /translation="MKQLFDTIAQAEMLGFAFLVIAAMVICVAIIFFFALIIVLDKKS
                     IPVLRPARELDILKKHMLDIKANFPAYVNNILTLDEGKDLKKSNEPLDIGVIILKNYG
                     NSSAVDIQVTHIGSYKVEEDFSNKYVSLEPGESVAVLVYIPKDLLSSVKVSLVKVKSK
                     NYANSTRTEKFSIISDSELSYRISEKCTLHPFIQM"
     gene            complement(33389..34183)
                     /locus_tag="PLF14_04520"
     CDS             complement(33389..34183)
                     /locus_tag="PLF14_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007931531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HOT70594.1"
                     /translation="MALKDIKCFVLDMDGTIYLGNQLFDYTKEFLATAEQCGIDIWYY
                     TNNSSKNAKFYINKLKKMGIVCEERKMLISNQVIIKHLLEHHQKSRVYVAGTQYLIDD
                     FEEAGINVTDTDCDVVVMGFDTTLTYEKLVKACDFVRYGATLYGVNMDYNCPTETGFI
                     PDCGSICQLVYASTGVSAEFFGKPSKHTADFIFEKTGYDKKSLAIVGDRVYTDIALGA
                     YNGFTSILVLSGESSLEDVKNSDAKPDLIYASLKEISEDLKKIYKL"
     gene            complement(34194..35261)
                     /locus_tag="PLF14_04525"
     CDS             complement(34194..35261)
                     /locus_tag="PLF14_04525"
                     /inference="COORDINATES: protein motif:HMM:NF008519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOT70595.1"
                     /translation="MDEKKIFDIAIIGAGPAGSLLTMLLANQGHDILLIDKRNLDGYP
                     PVRHKACGGLLAPDAQRVLASLNLNLPNDVLANPQLFSVHTVDLNTGLRRDYQRFYLN
                     MDREKFDRYLSSKIPENVCKEYSAVVNKVEYENDLYTIHTGTGKTYNSKVLIGCDGAG
                     SVVRKCFFTKEKMPFKHYYAIQHVYKCDDAGAKYYAIFDKTLTDYSGWAFPKDGTFTI
                     GMAFPDGADGNRLFAQLIEKMKNDGLSIGQAVNKEGSLLLRPDGYPKTFSKNNNTLFL
                     AGEAAGYVSPSSAEGFSFAFYSACALAKSFEKGGNISKNYRHETRKMRIKLFFKVIKA
                     HLLYNQFIRKIIMVCKIKAIR"
     gene            complement(35261..37672)
                     /gene="leuS"
                     /locus_tag="PLF14_04530"
     CDS             complement(35261..37672)
                     /gene="leuS"
                     /locus_tag="PLF14_04530"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOT70596.1"
                     /translation="MVMEYNPKEIEAKWQKIWDDLKCFNAKDDFSLPKYYALVEFPYP
                     SGEGLHVGHPRPYTALDIIARKRRLQGYNVLFPMGWDAFGLPTENYAMKHKLHPAAVT
                     KNNIARFKKQLKSIGYSFDWDREISTTDPKYYRWTQWIFLQLFKHGLAYKKNMSINYC
                     TSCKVGLANEEVVDGRCERCGAEVVKKMKNQWMLKITDYADKLIEGLTDLDYLESIKA
                     QQINWIGKSTGAEVYFKLTNISEPLVVYTTRPDTLYGVTYMVISPEHPYLEEYKLLIE
                     NIDEIREYQTESSKKSDFERSEIAKEKTGVEIKGIKAVNPVNNKEISIWVSDYVLMSY
                     GTGAIMAVPGHDKRDYEFAKKFNLPIIEVVSGGDLSVEAYTDIEEGVMVNSGIIDGLE
                     VKDAKVKIIEYIESKGIGKAKDNFKLRDWVFSRQRYWGEPIPLVYCDTCGWVPIEESE
                     LPLLLPDLETYEPAEDGESPLSKITDWVNTTCPKCGKPAKRETDTMPNWAGSSWYFLR
                     YIDPHNDEAFASKEKIAYWMPVDWYNGGMEHVTLHLLYSRFWHKFLYDIGYVNTPEPY
                     SKRTAHGMILGENNEKMSKSRGNVVNPDKVIDDYGADTLRMYEMFIGDFEKSAPWSEN
                     GVKGCRRFIERAFKMTDFMTEEDNYSKDMETLMHQTIKKVTKDFEIMKFNTAIAQLMA
                     FTNAIYAKGSITKKEFADFLILLNPVSPHVTEELWSLCGFPGYIHQQKWPQYDEAKTV
                     EDEIEIVIQINGRNKTKIFISSDMSREDMEQLVYKNQEILNVLEGKQPKKVIAVPKRL
                     INIVV"
     gene            38121..39851
                     /locus_tag="PLF14_04535"
     CDS             38121..39851
                     /locus_tag="PLF14_04535"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOT70597.1"
                     /translation="MNKMKLISLVLCLVLVCSFVFTACDKKPTETATQQPTETSTQTE
                     TPTETQIDTTPAVVEKPAVMDITTLYYYGDATENAAEKLAYKNYLNRHYGFTINIHAH
                     ARDMYIETINLRAVSGDLTGIVRLFAGTDGIKWYQEGIVLAIDEFLADNEVWNTIVPE
                     HWKETFSYDGAVYGLAFGGDGTPSWFSRTMRGDWLDAVGMSRPSTIDEFYEVSKAFTY
                     NDPDKNGDNDTWGFCSRHVWLMQDIFKAYGCTPNWESALVPVWNPRDDIWEDPVIKPV
                     MADACTFLRKCYVEGIIHPELFSMSSTNVRNLMSNGHAGSCFYWDHWLIVWENAVKKT
                     VGSHAYMVAVGALSGVVTHKINCWKTEIGSPYVLMANTAQPKEVINWFINLAYGTAED
                     FFTFRYGIPQTDKKAKTGFLLDGMTVYLLHFKADDQGSVTSSGCPALVGGHPGYALDI
                     GEYGYKSAYYSGNPTWDSEQVTVTAANLQRRFEVFNEFNDGRLYLLTGDYEQPTNPEY
                     TNNMTEWNKAGRLYVSSVMVDGVDTTTALKTYLDTVMAFDPQTTLDFMNAKLGKTSNQ
                     DYRKLWNSLN"
     gene            40344..42044
                     /locus_tag="PLF14_04540"
     CDS             40344..42044
                     /locus_tag="PLF14_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70598.1"
                     /translation="MVLCLVLVCSLVFTACDKKPTETATQKPTETSTQTEQPTETATA
                     PAEVEKPAVLDITTLYYYGDATENSAEKLGFKNYLCRHYGIQFNIHAPARDMYIETIN
                     LRAVSGELTGIVRLFSGTDGIKWYQDGVVLAIDEYLKDNEVWNTIIPEHWKETFSYDG
                     KVYGLAFGGDGTPSWFSRTMRGDWLDAVGMKKPSTIEEFYEVSKAFTYNDPDKNGDND
                     TWGFASRNIWLMQDIFKAYGCTPNWEADLVPVWNPRDDIWEDPVIKPVMAEACLFLRK
                     CYNEGLVHPELFSMSSTNVRNLISNGHAGSCFYWDGWLITWENAVKKTAGNQAYMVAC
                     GALSGYVTQKINCWKTEIGSPYVLMANTQQPKEVINWFINWAYGSAEGFFTFRYGVPQ
                     TDKNAKTGFMLDGMNVYQLHYKADNEGNVKSTSCPAIVGGHPGYALDIGEYGYKSAYY
                     SGNPQWDADQVQINAANLKRRYDVFHEYNDGRLYILTGEYEQPTNPEYTDHISEWAKA
                     GMLYVSSVMVDQVEPKVALKAYLETVMAFNPQATLDFMNAKLGKTSNQNYNEVWNSLN
                     "
     gene            complement(42160..42639)
                     /locus_tag="PLF14_04545"
     CDS             complement(42160..42639)
                     /locus_tag="PLF14_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70599.1"
                     /translation="MTETNNQQNNQDIQETAEMENNNVVEETIVDDVQQDNEQQEYQQ
                     ETYQQQTYQQEAYQQPVYQQPAVTKSDTSKTISILDWILTIIVMSIPVVNIIMLFVFA
                     FAGEKESKKNLFKAQIILMATAVIAFIVFLIVFVVFMGHRLPELLDQLNQFKEQYGY"
     gene            complement(42689..43120)
                     /locus_tag="PLF14_04550"
     CDS             complement(42689..43120)
                     /locus_tag="PLF14_04550"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HOT70600.1"
                     /translation="MLRKVWERLLKDIKKAWIPVTACIAYVVIMTLTVGEVCPSKLLF
                     GVPCAGCGMVRAFELLITFRFKEAFIMHPGIYVILIAFIIFIVMRYFLNSNVKQIKLL
                     ATVTLSLLFVIFVVRAATSFGTEPLTIYNNAVLLHFIRLFY"
     gene            complement(43122..43718)
                     /locus_tag="PLF14_04555"
     CDS             complement(43122..43718)
                     /locus_tag="PLF14_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HOT70601.1"
                     /translation="MDINKVLDNLRKNNMNAVFAENRDCARELVKEMLFEDASITTGG
                     SVTLDQCGITKMIREDRYRLVERGDKAAMFSADFILSGTNAITEDGKLYNVDGYSNRV
                     ACICHGPKKVIIVAGVNKIVKDIQQAVLRVKHIAAPKNCIRLSRNTPCARTGKCISDD
                     INKTCNTADMICCNYVISAKQREQGRITVIIVNEELGY"
     gene            43882..45678
                     /locus_tag="PLF14_04560"
     CDS             43882..45678
                     /locus_tag="PLF14_04560"
                     /inference="COORDINATES: protein motif:HMM:NF021355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HOT70602.1"
                     /translation="MKKLIAVLLILILLLTGCTDVTQPSPQPSAVVRDESIKSIESLS
                     QLKSLLDASKDNIFSRWYRSVGSALLAKDANGPDYAVESVTATGDASQSHSETNTQVA
                     GVDEADTVKTDGKFIYSISSYGFVNIVSVDSNGYLELVKQIKDFGKNFSPAEIYVDDQ
                     FLIVIGTSYDMEGSYSAAYIYDKTDIAELKLLKDVMIKGYYSDSRKIGNSLYLICNYT
                     PYYYYLKDTDLLPCYIENGKTTYQSPSDIFYFDETECSSYLILGAVDLSDLTKDINLN
                     TYLGSGQTVYMSHENLYVTYSQYPYKPVRKILPEEDSYYYKEETVIHKFRADNAVLNY
                     VATAKITGYPINQFAMDEYNGFFRIAVTDNLEGKSVNNVYVFDENLVQTGTIQNMAPG
                     ERIYSVRFNGDIGYMVTFVVVDPLFVMDLSDPYNPKVDGELKIPGYSTYMHMYSDTLI
                     LGFGMDTTEQDGRALNQGIKIAMFDVSDRANPKQLFTTSIGGRGTWSELNYNHKAFMF
                     DREKDIFTFPASISGNNYEMKTQGLVVCKIDMQNNRFEVLDILSQDYFTNWSNYVERG
                     MYIGNTLLTISKNAIYSYNYTTLQFIDNVLFD"
     gene            complement(45653..46930)
                     /locus_tag="PLF14_04565"
     CDS             complement(45653..46930)
                     /locus_tag="PLF14_04565"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HOT70603.1"
                     /translation="MEQYLDDMIEDLRGLIAIKSVKDEPAPNAPYGEGTLKALEYMLN
                     LGKKYGMKAVNIDGRAGYLEYGEGKHMIGVLLHLDVVPEQDGWTSDPYTLTERDGFLY
                     GRGVSDDKGPAIAAFYALVDLIKKGEIKGYRVRLIFGLDEECGSSCMAHYVKTEELPT
                     MGFVPDADFPVIYAEKGILTVAIGGEGNKDISLQAGDRPNVVPGKCRVEYEGKKFVYE
                     GKPAHGAAPFNGINAIKIAIENNPVLTGIPIIEFFRDALADKHYGEGLDIDCKDETGR
                     LTVNPGVMVIDKEKSVCVLNIRYPVTYDYKEIVEKIEKVCGRYDLRAVYTDNNPPLYA
                     DKNSVLVSTLLSVYREMTNDYTEPIAIGGGSYARSMPNLVAFGMNMPGDLESAHQANE
                     CIKKQRLYEGAAIYREGIKRLGETLINQKGHCQ"
BASE COUNT        13528 a         9882 c         9270 g        14206 t
ORIGIN      
        1 tgccccatgc tcatcccggg accgaagtcg gtatatgtta ttcctttggg tgccatcact
       61 tccatcaggg ctcttactat ggaatcggta ccgggatccc atcccgccga tattattgaa
      121 gctacattgt ttttctttgc aaccttatcc agttttcttc tcaaatcaca tattcccgaa
      181 tgaatgtcat atgcatctac agtatatatt cctttattaa gaagggattc ggccaccata
      241 ggtaccactc tgctcggagt gcacaatatt gccacatcaa aaccgctgag cttatctata
      301 ttatccacga taggatagtc aattttaatc ttggacaaat ccatattagt ctcaacaaca
      361 cctgcaacag caaaatcatg gctttgagac aaggcttcca ataccgctat accgatattt
      421 ccataaccta taactacagt ttttatcata attattcccg ctcctttatt cactcttttg
      481 ttaaagtata actctgataa aataaaatga caacctttca taaaaatatc ccgcctccgt
      541 agttattccc tactttggcg ggatatctgt taagtttata tatctcccgt tctacttttt
      601 acccggcttc catctcatcc caaagacaag ggatgttgtc gtggcaacag tcattattgc
      661 cgagacaggt gtggtttcat ttaccattcg catagttaaa ctcaggttgt ttgttgaaaa
      721 cagatatact attgcaacaa ccgccaatac tgccgtaatc aacttgaata tcttgtttct
      781 cacaaaatgc gtaccggagt catccactct gaccgattta atcagaagct gaataagcat
      841 tgccaatgcg gaaaatgccg ttgaaatcac actcaccaat gaccaggatt cttctttttg
      901 ttcttcagga gtctcctctt ccgtatcagg accgggtgtg ggaggcggag tttgaccgga
      961 atcttccttt accgttactt ccacttccat cacttccgta gatccgtcgg gatatgttac
     1021 ggttatttca cgcgtatatg ttccgggaac cgttgtgtca agcggctgtg ccgatgtatc
     1081 ggtaaccgtt gtgccttcgg gcagatcttc cagattttcc acgacatttt tccaatctat
     1141 ttccttgccg tattccaccg tttcactgcc gacaacggga ttatacctgt cactctgctg
     1201 ctgttcttcc accgtaacag taacatctat cacttcctcg gacccgtcgg gatatgttac
     1261 ggttatctga cgcgtgtatg taccggggac ggttgtatca agcggttgct ccgacgtgtc
     1321 ggtaaccgtt gtaccggcag gcaactcttc cagattcttt atgacatcct tccaatctat
     1381 ctcttcaccg tatcccaccg gttccctgtc cgcaacagga ttatatctgt cactctgcgg
     1441 ctgttcttcc accgtaacag ttacatctat cacttctctc gagccgtcgg gataggttac
     1501 ggttatctga ccctgatgtg tgccgggcac cgttgtatca atcggatttt ccgatgtttc
     1561 ggtaacagtg gttccgggcg gcaagtcatc tatattatct ataacatcct tccagtctat
     1621 atcctctccg tatcctacgg tttcgtcatc acgtacaacg ggattatgta catcactctg
     1681 cggttgttct tccaccgtga ccgttacatc tattacttcc tgcgatccgt cgggataggt
     1741 tattgttacc tgcccttgat gtgtaccggg caccgttgta tcaataggat tttccgatgt
     1801 ttctgtgaca gtagttccgg gaggtaagtc atctatatta tctattacat ctttccagtc
     1861 tatatcctct ccgtatccta ccgtttcgtc atcacgtaca acgggattat gggtatcgct
     1921 ctgcggttgt tcttccaccg tgaccgttac atctattact tcctgcgatc cgtcgggata
     1981 ggttattgtt acctgaccct gatgtgtgcc gggcaccgtt gtatcaatcg gattttccga
     2041 tgtttctgtg acagttgttc cgggaggcaa gtcacctata ttctctatta catccttcca
     2101 gtctatatcc tctccgtatc ctaccgtttc gtcgtcacgt acaacgggat tatgggtatc
     2161 gctttgtgtc cgttctttca ctattatcct tatatccgat acaattaccg atgcatcggg
     2221 atatataact gccagttgtc ctctatatgt tccgggaacg gttgtatcaa tttcctgctt
     2281 atatatttcc ataactctcg ttcccgcagg taaatctttc agattcaaca cattatccgt
     2341 caaatctatc actccgccgt atgccactat ttcatctatc acaacggggg cattggcctc
     2401 atcatcggct aaataatccg gctcgggctg cactcttacc tgcacattaa ctacatcggt
     2461 tgtctggtcc gggtatgtaa ccgttatctg accttggtat actccgggcg atcttgtatc
     2521 aataggcgga acggttgtat cggcaataac cgtatttaag ggtaattcat ctgcgttggt
     2581 tacattatct ttcagattaa tcactccgcc gtactccact atctcgggaa caacaacagg
     2641 ctcaaatttt tcattgagag gttgaggcag taccgttacc tgaacattga ttacagtcgt
     2701 tgtctgatcg ggataggtaa ttgttatctg tccctggtat gttccgggca acctcgtatc
     2761 tatcggtggt gccgtagtat cggtaaccgt cgtttgatcc ggtaagttat ccagattggt
     2821 aacgttatcc gttaaatcta ttacaccgcc gtacgccacc gtttcatcaa ccgcaaccgg
     2881 attatatgat tggttgtcgg gaatatccgg ctgatggtta acagtaacct gaacattgac
     2941 cacatttttg gtgccgtcag gataagtaac ggttatctca cctctgtata atccgggtac
     3001 cattgtgtta atcacgggag ccgttgtatc ggaaatatcc gtgttatccg ggaaagtatc
     3061 ggcatttgtt acattatctt tcagattaat cacaccaccg taatccaccg tttccggaac
     3121 tacaacaggc acatatttct cattgtgagg ttgcggctta accgttacct ggatattgta
     3181 tacatcggtt gtctgatcgg gataagtaat ggttatctgc ccttggtatg ttccgggtac
     3241 ctttgtgtca ataggttgtg cagtcgtatc ggtaacttgt gttccgtccg gcaggttttc
     3301 aaaattcaaa acgttatccg ttaagtctat cactccgtca tactccactg tttcatcaac
     3361 cacattcggg ttatatacct gattatcggg aatatccggt tcatggttaa ccgttacttt
     3421 gacattgacc acatccttag ttccgtcagg ataagtaacg gttatctggc ccggatatac
     3481 tccgggtacc gtagtgtcaa ttaccggatc ggttgtgtcg gaaatatccg tgttatccgg
     3541 gaacgtatcg gcattggcaa cattatccaa cagattgatt acttcgccgt actccactat
     3601 ctcggggaaa acattcggct catatatctg attcataggt tgcggttgta cagttacgtc
     3661 cacattaaac acatcgctgg ttccgtcacg gtatataacg gttatctgac ctgtatgctt
     3721 tccgggtacg gtagtgtcaa tagcaggatg cgttgtatcc aaaatcattg ttccttcagg
     3781 taactgctga atattggtta cgttatcctt gagatttaca actccgttgt attccactat
     3841 ttccggtact acaacaggtt catatgtaac attctgtgcc tgactgaccg ttacctttct
     3901 ggtcttttcg gtaacatttc cgcccgaatc ctctgccctg tatgtaacgg tatatgtgcc
     3961 cggtacggtt gtatccacct taggcgtaac cgtaaaatca atttctccgt cttcatcatc
     4021 ggttgccgta acacccgctt taggattaat aagggcaccg tacataagag tgacatcatc
     4081 aaagtcgtta aataccggcg cgtcatccac ataatagaaa ttgaaaacat tatctgttgt
     4141 accgtcaata gtcaccaaag tatcctgatt tgctacttta tcatatgctc ctccgtcggg
     4201 aagaggtcct acatcatgct gtcctacggg taagtcgcct gtcttaggta atatattagg
     4261 tacgggttta gtcgtgttat ccccgtttgc aagacggata aagtaattta ctctgtacct
     4321 gtagttaacc tttaccgtta ctttaacatc atcctttgta ccgtccgggt atgttaccgt
     4381 caccgaaacc tcattatctc ccgatgtgtt accgtccggc agttcggcta aattaacagt
     4441 taacgcgggc tgaagctgtc ccggaggata attatctacc gcaaccgcag cctttacttc
     4501 ggcttcggtt gtagccacac cgaatttctt aaatatgtcg tttacagtcg gctcataatc
     4561 gtcagccaga acattcttaa caataacatc ggctgttttt gaatagccgt tcaatgttgc
     4621 ttttacttca atcacggtac ctttattttg tttatcaacc tttaaagtaa agttaccttg
     4681 tgcattaaca accgtttccc atgtcttggc atcttttgtt accgagattt tagctccggg
     4741 atcggcttta ccggtaatga ctgtcgtagc ggcatgagct ttactgtcgt taatagcgtt
     4801 taaatcatat ggagctcgca gcagtaccgt tattgttctg tttttgacag tgacgttccc
     4861 tgatgtatcc tccgctctgt atgtcagaat atagtttccg ggtcgggttg tatttacctg
     4921 agatgtgacc gtatacccta tttctccttc ttcatcatcg gttgctgtaa ctcccgctct
     4981 aggattaaaa ggtgtaccgt attcaataat actatcatca cagccgttga acacgggtgc
     5041 atcatctaca taatagaatg taaagttgtt acccgatgtc tgaatggttt taacggtatt
     5101 ctgattggct gccggagcat attgccctac tgcctgtaat tgaccgaaat catgcggtcc
     5161 taagggcagg cttccggtaa tatcgtttat tcccggcact tgtttgtttg tattatttcc
     5221 gttttccagc cttatgcgat aattcaccgt gtaacgatat aggacggaga cctttactgt
     5281 agctgcatta gcggaaccgt ccgggtaggt aacggttaca ttaattaaat aatctcccga
     5341 tgtgttaccg ttgggtattg tctgattcgg attaactgtt acttggggtt gttgcggtcc
     5401 ggccggataa tcaggtaccg taactgcatt gattacttcg gcttccgttg taggcacacc
     5461 gtattgttta acaatattcg ttattgtcgg taaataatcg tctgcctgga catttttaac
     5521 aacaacattt gtcgacttta catggccgtt caatgtagct tttactgcga ttactgtatc
     5581 tttcagttgt ttatcaatac tcaaagtaaa attaccttgt gcacctacct gtacaatcca
     5641 tgtcttgggg gctatttcta ccgaaataac cgctcccgga tccgccttac cggtaataac
     5701 cgtcgtggtc ggatgggttt gcctgttgtt aatggaggta agattgtacg gagcccgcgg
     5761 caataccgtt accgttctgg ttttgacagt gacattaccg gatgaatcct ccgctctgta
     5821 tgtaagaata tagtcatcgg gtcggttgac atttacttca tttgtaacgg tatactcaac
     5881 ttccccttct tcatcatcgg ttgccgtaac tcccgctttg gaatcaaaag gtgtaccgta
     5941 ttcaataacg gtattctcac agcctgtaaa tacaggtgct tcgtctatgt aatagtaagt
     6001 gaatacatta ccttctgtat gaatggtcac atcggtaacc tggttaggca ccggtgcata
     6061 ttttccgata ggctgcaatt gtccgaaact atgaggtcct tcgggtagtt gaccggtctt
     6121 aggtaattta tcaggcacat gcttatccgt gttattcccg ttttccagcc ggatgaggaa
     6181 atatatcgtg taatcatata caggttgcgg caacaccgtt atcttccttg ttttaacagt
     6241 ctcgttaccg gacgaatcct ccgctctgta tgtaagagta tattctttag gtacgctcgt
     6301 atcaattgcc gcaggtgtaa ccgtataatc aatttctcct tcttcgtcgt caattgcctt
     6361 aatacctgtt ttcggatcaa atgcatcatt atatcctaca tagatatcct caaactcggt
     6421 aaataccgga gcttcatcta tatagtagaa agtaaataca ttaccttccg cctgaatggt
     6481 cacatcggta atctgatttg ctgcaggagc atattttcct atcgcctgca attggaaagt
     6541 atgcgctcct aaaggcaggc ttccgatttc ctgcggctta tcgggtaccg gcttggctgt
     6601 attattaccg ttttccagcc gtataaggtt atttatcgtg tactgatagt aaaccgatac
     6661 cgttacaaca tccttattgg ttgaaccgtc cggataggta acggttacat taatgggata
     6721 atctcccgat gtattaccgt ccggcagagc ctgaccttga tcaacggtta ttacaggctg
     6781 tgcctgtcca ccgggataat tcggcacctt aaccgcagcc tttacatcgt cttggcttgt
     6841 agctacgcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg cggtatttta
     6961 ccgtatactt atagtaaacc gataccgtta caacaacttt atttgttgaa ccgtccgagt
     7021 aggtaacggt tacattaacg ggatgatctc ccgatgtgtt accgtcaggc attatctgac
     7081 cctcatcaac ggttattaga ggttggtctt ggcctgcagg ataatcaggt accgtaaccg
     7141 cagcttttat ttctgcttcg ctagtagcta caccgtattc cctatctata tcagccactg
     7201 tcggcagata atcgtcggct aaaacattct gaacggtaac atccgctgaa gttgtctgac
     7261 cgttcaatgt ggatgttacg gtaattacct cacctttatt ccgcttatca atatttaaga
     7321 taaaatcacc gtttgcatcg gcatcaacaa accaggtcgt tccctctatt gttaccgaaa
     7381 ttgtcgctcc cttgtccgct tttccagtaa ttcttgtctt atcctgatgg gcctgacggt
     7441 cgttaatagc ggtcatatcg tacggcgctt gaggttttat tgttaccgtt ctttgtttgg
     7501 taacgacatt tcctcccgaa tcaataactc tgtattcaag gttgtatacc acgggattgc
     7561 tcgtattaat actgtccggt gtgacagtaa atgaatcggt taaatctccg tcttcatcat
     7621 cggttaccgt aacacctgtt ctgagatcaa aatccgaacc gtactcgata gcgacattat
     7681 cacagccgtt aaatacgggg gcatcatctt tgtagtagta ggtaaaattg ttcccctctg
     7741 tctgaataat cacatcggta tcctgatctt ctacgggagc aaactgccct tccgtcttca
     7801 atgcttcgaa actatgctct ccgagcagca gacttccggt aatatcgttt tttcccggta
     7861 cagctatgtc cgttttagtt ccgtcttcca gctgtctgcg gtattttacc gtatacagat
     7921 atttaacctt tattgttacc gtagctgcat cggttgtatt atccggatat gtaacggtta
     7981 cattaatagt ataatctccc gatgtgttgc cgtccggtaa tgtctgagct tcgttaacag
     8041 caactgtcgg ttgttgttgt ccctgaggga aaccaggtac cgtgacggca gccttcactt
     8101 catcctcgct tgtagccgta ccgtaatctt ttataacatc cgttacagtc ggttcataat
     8161 cgtcggcctg gacattctta accgtaacat cggcggattt tgtatagccg tgcattgtgg
     8221 attttacttc gattacacta cctgcaggtt gtctggtaat cattaaggta aattcacccg
     8281 attcgcctac aggcatactc cattgagaag aagatattgt taccgataca gttgctccgg
     8341 ggtcggcttt tcctctgaca gcagtctttg cagaatgggc tttactgtcg tttatagcgg
     8401 tcaaatcgta cggaggttgc ggttttaccg tcacaactct ctgtttgacg gtttcgtttc
     8461 ctcccgaatc ggtaactctg tatgtaagtg tatattgtcc cggtttactt gtatctatac
     8521 tttccggttg gactgtaaaa acattgatgg atatatcttc atcatcggat accgtaacac
     8581 ctgctacggg atcaaaaacc gcattatgct caatgctgac attaccgcaa ccgacaaata
     8641 ccggggcatc atctatatag tagaaagtaa atacattatc ctgttcctta atggtcttta
     8701 cggtatcctg acctggtact acagcatact ttccaccttt gggcagaggt cctacatcac
     8761 ggtttccgag aggcagactg tcaatcttcg cttccaatcc cggtacgggc ttgaccgtgt
     8821 tgtctccgtt tgcaagccgg atgcagtagt ttaccgtgta ctgatagtaa accgatacct
     8881 ttacgctgcc tttattggtt gagccgtcct ggtatgtaac ggttacctta atcatataat
     8941 ctcccgaagt gttgctgtcc ggcagcgtct gacctgaatc aatagttact acgggcggtt
     9001 cctgtccggc aggataagta ggtatcgtaa ccgcagcctt tatttcctct tcggttgttg
     9061 acacaccgta ttttttattt atattagtaa tagtcagcgg ataatcgtct gccataacat
     9121 tctgaacagt aacattggca atggcagaat aagtattcag cgttgatttt acaggaataa
     9181 ccgtaccttt atcctgcttg gctatctcta atgtaaaatc accgtgttca tccgctgtga
     9241 ccaaccatgt tgtttcccct atttttaccg agatatccgc tccgggatcg gcttttcctt
     9301 taataaccgt cgtattctca tgcttctgac tgttgttaat ggcggtcata ttactgggtg
     9361 caacaggatc gggcagttta gggaccgtag tggtaaccgg taccgaggca ttgcccaaat
     9421 agtcataata gactacctca ataacctgga attcctcaaa tttttttgcc cctgtaaatt
     9481 cataggttaa gactcctaca gtcttctcaa ccttattatc ggggtcatta gggtcaagca
     9541 ctacccaaac tttttcttca ctttcggtaa aagtataacc tccaccgcaa atccatatgt
     9601 ttgtcggttc acctgtctca ttattatatt cccgtttcgg ctcacactct ataaacgctc
     9661 ctattttaag tttaatcttg tcaacatcct taatatcggg aagtaccgct tcaactgaag
     9721 tgttggaaat ccttaatgta ttggtatttg ccaaaggctg atccacacat aaaggtgtcg
     9781 gagctacatt atctatgaca acggcaccgc cggttgatat tttcttttta ttctttttgt
     9841 ctatagtttc tcccgcggaa tcggtcttat ctgactccaa ttcgtttgct ttttgctggg
     9901 ttataaagat ttttttgtct ttgaaacgta ttatttcctc tttgggaaca ataatatctt
     9961 tagtaccgtc cgcatttgtt atcacacttc cggtaattga cggaattaca atctccgtat
    10021 atactgtcgg actttcttcg ctgtagaacc taagtattcc gttctttgta ccctgaacag
    10081 tcaaagaaac atcaccgtcg gcattattgg cattctgggt aatggaagta atggcgtctg
    10141 tagtgggttt ttgagtgacg gtaaagacaa tattctttgt cgacacattt ccgaagacat
    10201 cttccgcagt tacggcaatg gtatatattc ctgtctgcga tacatcaaga acatccgccg
    10261 tcttaccgga aaacttacct gcagcataat caaatgcgat accgtcggga agagaaaccg
    10321 tcgctccccc ctttgtatac ttaaaagtta taacgtcctt ggcatctccg gttgtcacgg
    10381 caccttcagc ggctcctatc agattacctg tcaaaactcc gaaggagtcc ggtgcagtaa
    10441 tatccggtcc cgttaaatcc atcgttacag tcggaggacc gaatgtagta tccttaaaag
    10501 tcaaggcggg gtcagtacct tgaatacaat atgttgtacc ctcattcaat gttccggcag
    10561 gtactttgaa agttattttt tcatccaaat cgggtgattc attgccggaa accgcctgga
    10621 aagtcccgcc ttgcatatat cccaatgtat acttcgcacc gttataggta cctgcaggta
    10681 tcttggcggt aacgacattg tgagtaataa gaccgttgtc atccttgatg tgcggctcct
    10741 gcttaagatc ggtaatcggc ggcaggtaat cataggacat aacttcacta aaagcacagt
    10801 ccgaatcggg cggaatgtcg gtattgttaa cagtgtataa cgcatacacc ttcttgcccg
    10861 ccttatccga aaaatcatat gttaacggga tggtaataat taattcatca tttacttcac
    10921 ttatagtaaa attgtcatca ccggaagtcc atatcgtatc acctttactg aaccatatag
    10981 gattttcagt ataatccgga agttttaagc aaagatactt cgcaccgtcc ttaggactgt
    11041 caatcttaat ctcggtatga tttgtctgaa ttctgtgaat taaaggcagc gcaaggtcaa
    11101 ccttaaaatt ccttaatgtg aaatgataat tctctgttac tttaacggtc tgaacagcat
    11161 caggctggtt gctgtttgtg accgccaaat aggaatgtgt tttgttagtt tggtctttta
    11221 aggtgatagc cgtatatttt gaaagattat cgcccttatt gagcattgtg atatacattg
    11281 aggaccatgt tcttttgttg ttgaagtgat caataatttc ctcttccgta tgaaaacctt
    11341 cctgttccgg gcttataaac tccgtcataa gataggaata cttccgaccg ttggcatcat
    11401 gaccttcctg tggcgcccac tgccagttta ctccgtcttc cgttgttctg tagaaacctg
    11461 gaattatttc atactttgtc ggatccaaag tcttcccttc ggttgttctc ataagcacca
    11521 tagccacttg gctgttgccg aaatgtgcaa ccgcatcggg atcgggtttc tgattattgt
    11581 taggatcttc tatccatttt tcatatgccg cctttgctcc cgaaggatac cataatttct
    11641 ccgcagtcat tactatcaaa ggcttatact gagcataaag attgagagtt gcaatgccat
    11701 tatcgttagt caaatcggct gaaacatcct tccatggatt atcatgaact acaggatttt
    11761 gaggtagctc atttaactga agttgaagct tccctccgtt caaccttatc ttgcttgcat
    11821 tggtatataa ctgatgtact aaagttccgt ccgggactac cgcctgctcc gcccaaccta
    11881 caaagtagca atcggccttt tgaaaatcgt tatcatacag tatattaccc ggttttccga
    11941 ggtatgttgt taattccgca gtattatcac ccgttatatc cgcaacgggt acatatatta
    12001 tgtggctgac attcatgtcg ccgccgttgt tgttatgaag tactatttta tactgttcta
    12061 tagtgttttt ttcccaaacc gcataaacgg ttctgccctg gtctacggga gaggttgcag
    12121 taaacttata gttatcggta ctttcccatt gtgccacatc ggtaagtgcg ggcaaatcgg
    12181 caaaggtcgt tgctcccatg gaatccacct gcttggtgga ccaaccgagg aaattgtaac
    12241 cggcacgtgt cggatcggaa cagaaagttc tgtcttcaat atctcctagg tcggtaaggg
    12301 ccttaccttc ataatcggaa agatattcaa tcgtaccctg ggaaaccttc agaatattgg
    12361 gtccatcaaa accgttgggg ctgtaaccgg ggtcatcgta agccttttga tttatgggca
    12421 caatgcgcac cacagtgtta ccggttccgt catttttatc gaatgtcact tttgcctggg
    12481 ctctgtaggt ggatgccttt gatctcataa aacctattgc cgaattggaa aaggacagag
    12541 ccatatcttt ttttaagctc acatcggcag gtatatccag agtaaatgca taaccttgaa
    12601 cattactccc ctcggcagta aaatcttgaa gaatggagga agaattggct tctctgaaaa
    12661 ccgctccgtc cgatgctttt cttaccgata caagagccaa aggatattta ctgtgtccgg
    12721 taatagacgc actgtccgtg aaaaactcgt caacatgagg agtgtttgca ctgcggttta
    12781 ttatgatacc cgtacgcaca ttggaatgag ccaggttata tattttattc tcattctccg
    12841 cgtgttttgt taagatatat tccactctgt tctcatcgta cattccggta actaccgtca
    12901 tttttacctg attgggcgat gcagtatcga acacggttaa gtttacggtt tctcccgccc
    12961 ggagtgtcag acccgtttcg ggtgttaaaa catgtccgcc aaatattagc ccggaataga
    13021 atgtttgtgt ataaccttca ccgtagtcgt tagggtctac acccgtactc atctgaccgt
    13081 ctacttttgt ttttaaatta cctattaaac gttcatagtt gttgttcgga ccggatttaa
    13141 aaacggaacg cgaagtttcg ggcaactcaa atacaagcct tgctcctttc ggaatgacaa
    13201 tattctgagt aggagtcaat ctgtactcag tctgcgtctt ttccgtaggt ctgtcggtta
    13261 tgtaagctgt ggaaatgctt aaagtcgagt aattgccttg tgcaaacaag gcatccatct
    13321 tattcttatc cacattgtag ataaagcgta caaagtccga ctgttttcta tatccgtgta
    13381 aatacacgtt ctttatatta gttgtctgat cggcaggcac aacagcctta ttcactacgt
    13441 cggttttaaa cccggcaggt accatcatgt aagaataggt gcctacggtc ggagttgtcg
    13501 gtatatagga aaattcatcg gctcttattt ccgtagatac actccaatta gttccgggaa
    13561 caccgtccaa atccgtcatt ctcatctgac ccactacccc gtcggcatcg ggcagtaaag
    13621 catcatatat tctggaatcc atgacctgac gtattcctat ccatgaggat aagaacaatc
    13681 cgttatttgc aaaagtacga taggtatagt gattaggcgc caccctgtag taaacatgaa
    13741 gtttcccctg agcgctgtca taaataaccg attgggccac cgttctcatc atgtccggag
    13801 gcaggtaagg attggcaacc agattactca tggaagccgt gtggaaagga gctattgttt
    13861 tcaaagtaac atcgtcattg tcggcactgg gatcataatt gtccagttct tcaaaatagt
    13921 tcaagtagtt tcccagcacg attcttccgt ttgcaccttt ttgattatag acttgattac
    13981 cggtattggt ataacgcaac tccaaagcgt aatcgtcatt caactgctct cttgtgaccg
    14041 tgtctttcag tattaaatag agtttagact tcatgtagtc actgttggca ggcttctgcg
    14101 gatatacctg atcaatagta aatttatata cattctccgt ccccgtgttg tctcccaggt
    14161 taaaggaaac ggttgtcgga tctttcggat aatacatttg aaagaaactg gcatctacat
    14221 ccaccttgtt tataagtttg gggtctatga aaatatgtgc attttgccaa ttctctctga
    14281 aaagtctgga tgctgaaacc tctctacccg ctgatgccgg aaacatgctt aactcaaatt
    14341 ccagcacaat cctgtcctgt tcatcataat agtaatggtg gtatgaaaaa ccggctgtac
    14401 ctataccggt atatgacgtt gactgaatag tagcatctaa aggccattcg tttaaaacct
    14461 cttcgtctgt agtccaggaa aaattcgtgt cataagaacc ttgtgactgc actttaccgg
    14521 gcatacctgc caaggctaaa atggcgatta ataaaataac catagacctt tttattaaat
    14581 ttttattcct tttaaaacct atctttttca atctaccata cctctcattt acttatgcac
    14641 tgtatattat aattaaaatc ttttatttgt tcaatatatg taacgaacta tttagtatta
    14701 agtatcaata atagtgtaaa ttaatgttaa atttacttta aaaccctacc atatcagtat
    14761 ttatacaaca caatatccgc tgttgaatgt aaaatagcaa aaaacaccga taaatacaat
    14821 aaacaacatt ttaaaatata ctgtcgtttg ctttttttac acatacttca ttgacacgga
    14881 aaaaattact aactataatc taacattatt agaaatatgt tagtaatcaa agaaggtaca
    14941 acatggcaag taacgataag attcaaaaaa ttataaggat aattgaggat attgagctgg
    15001 aaaaagtttt taaatccctg aatgacacaa tgaccggaat aggtgcagcg cttcatattc
    15061 tctatgaata tgacagaagt accacaagcg gacaactttg cgatttgctt ggagtaagta
    15121 cggcaagggt tgccgttctt cttaaaacaa tggaatcaaa gggacttatc atcaagagaa
    15181 aaggtgtcct ggatgcaaga accactgttg tctcactcac tgctttcggt aaggagacaa
    15241 cgctgaaaat acgggaagaa atgtataacc ggattaatcg gattattgat catgtgggct
    15301 ttgcacagct tgagcaattt ttcgctattg caaagcagat tgaaaaagcg gtaacgccga
    15361 ccgcaaatat attctgaagt cagtttttca agaagcagta aatctttctt gattttatga
    15421 acttagaaaa tcacggtata caaggaggaa caaaacttgc tggaacttaa aaatataact
    15481 aaaacctatc acacggtcgg ggaggatgtg gaggctctta aaggaataga tctcacattc
    15541 cgcgataacg aatttgtttg catattaggt ccgtcagggt gcggtaaaac caccatgctg
    15601 aatattatcg gaggtcttga ccagtatacc accggtaatc ttctgataaa cggcagatcc
    15661 actaaggaat ataaggatag ggattgggac gcttaccgga actattccat aggctttgta
    15721 ttccaaagct ataatctgat tccccatcag actgtattaa gcaatgtgga attggctctt
    15781 accctgtccg gagtgtccaa aaaggaacgc atcaaacgcg ccaaagaagc tctggaaaga
    15841 gtcggtcttg cagatcagat acgaaagaaa cctgcacaac tttccggagg acagatgcag
    15901 cgggtggcca ttgcacgtgc cattgtcaac aatcccgaca ttatcttagc cgatgagcct
    15961 accggagcgc tggataccga aaccggtatt caggttttgg attttctgaa agaaattgcc
    16021 aaggaccgcc tggttgtcat ggttacacat aactcatatt tggcagaaaa atattccacg
    16081 cgtatagtaa aaatgctgga cggtaaaata atcgatgaca gtatgccgtt agatatcgaa
    16141 gaaaaagaga agataactgt cgagacggaa agcagcaata aaaagtctct gcgaaaaaag
    16201 aaaccgtcaa tgtctttcgg tacttccttc gggttgtcgt tgaaaaacct tattatgaaa
    16261 aagggcagaa cagcgttaac cagttttgcc ggttctatcg gtattatcgg tattgcttta
    16321 atttatgcgg tatcacaagg taccaccgca ttcattgact cggtacagga ggaaaccctt
    16381 tcttcctatc cgttaacaat tgaggcgcag agtgtggatg tatcaacttt gctttccact
    16441 ttcatgggaa aagcacagtc ggtttccgag catgaggacg atgcaatcta ccaaaaagcc
    16501 atgctttatg aaatgaccaa tgcaatgaac tctatggaga cgcagaaaaa cgatttgaaa
    16561 tcttttaaag agttcattga aaaagaacga gccgacgaag aaaaccggct gcatacggca
    16621 ctttccggta tacagtatac ctatgaagcc gatcttctaa tctacactaa aaatgttgac
    16681 ggtaatatta tacgttccga tacggaaaca atgacactgg aactgatgaa aaaatatgcg
    16741 ggtgtggata tgtcttccat gctggcaatg cgctatcaaa ccgctatgag aaattcctct
    16801 tccatgactt tgtggaaaga attgctttcc ggtgataacg gaagaccggt acatgaaatc
    16861 attgaaaagc aaaatgactt gatttacgga agctggcccg cccgttatga tgaaatagtg
    16921 ctgtttgtgg atgaaaataa cgaaattgat gatgtgactc tttatgcgtt gggtctgaaa
    16981 tcggaagaag aaatggagaa actgatggag gcggcagtca ataagacgat tattgattat
    17041 gagataaaaa aatggtccta tgaggaaata tgtaatatgg attttcgcac catcctaagt
    17101 tccgactgct acacttacga tgaaaaatcc ggcacttata tcgaccttcg ggattccgaa
    17161 gccggactga aatacctgta tgaaaatgcc ttaaaacttc atgttgtagg cattgccaaa
    17221 cccggcaagg atgctgtttc atcctcaagc cgcagctata tcggctacac aggcaaactc
    17281 accgggtaca ttattgagca ggctgccgaa tcggatgccg ttaaagctca aaaagaaaaa
    17341 tccaacaccg acattttcac cggcctgcct ttcaaggata aggaaggtat ggtttcagcg
    17401 gagaagaaag cgagggattt caaagagtac atctcaggtc tggatgtttc cggtaaggcg
    17461 accgcatata ttaaaataat gagcattcct tccgaagaag ccactcagca atttgtctcc
    17521 gacactcttt ccggcatgaa ccgcatcgaa atggaagcta ctgtcgcaca ggctatggcc
    17581 gagcagatgg atgtggaccg ttccaccgtt acaggctata tttcttccat gagcgatgag
    17641 gatttacagg aattatttac caaaaagttg aaagaacagt ttcatgcaca gtatgctgca
    17701 caggtaagtc aacaaatgag cgcaatgacc agtgagcagc tggccgcttc tcttgatatg
    17761 gcgattaatc ggtatacgga cgaaatgtgt gcgcaatatt atgatgaaat tccggaattc
    17821 tccgattcca cctacgacaa caatcttata aagctcggat gtgtcaatct tgacagtccc
    17881 tcatccatta atctgtatgt gtcttccttt gccgacaaag agattattga agaagctatt
    17941 gcggaatata atcagaatgt ggatgactta aaggaaatca cctatacaga ctatgtaggt
    18001 cttctgatgt cgtcgattac gacaataata aatgcgatta cttatgtgtt gattgcattt
    18061 gtttcggtat ctcttgtagt ttcatcaatt atgatcggcg ttattacact catatccgtt
    18121 caggagcgca cgaaagagat aggtattcta cgtgccatcg gtgcatcaaa acggaatgtt
    18181 tccggtatgt ttaatgcgga aacggtcatt atcggcttca catccggtgt acttggtgtg
    18241 gttctcacct atctgctttg cattcccgtc aatttactgt tgcatcatct gaccggtctg
    18301 aacaatctca atgcaattct gccgctgccg acagcaattg ctctgattgt aatcagtatc
    18361 tttttaacgt tggttgccgg tattataccg tcacgcagtg cggccaagaa agacccggtt
    18421 gttgcattaa gaacggaatg acgctgtgaa gttttagcaa cgttttatgc tattcctcta
    18481 aaacagctct tgctttggca tatttctcct tattgcgagc aatatcttct gccgaaagtt
    18541 cattacaccc ctcgaagcgg ctctcatccg aattatgagc aatatcggct aacttgactt
    18601 tcaaagccac cggattttgt ttgatttcac gtaaatagtc caagtagccg gtcccgcttt
    18661 tacgagtcaa gaaacgcaaa gcctctataa cttctctcgg aaactctttt tccaattcct
    18721 caaaggttac agaggtatcc tctatcacat catggagcag ggctacgcaa acactgatct
    18781 cgtctgtcat ctgctcagca acatgatatg ggtggaaaat gtacggctga ccgcttttat
    18841 ccgtttgtcc gtggtgcgca tcataagcca aacgcaaagc cttatttgta aaaaccgtgt
    18901 aaatcatgtg ttatcctctc tctgtctatt ctattataca actttaaggc atctgatact
    18961 gattatgttt tccggagtat acatgctttt aaaataccga ccgctctttt tatttacttt
    19021 tgacgtgaat gaggtatcag attttacatg tcaattgtac aatcaaaacg attttgaaaa
    19081 tgattagaaa attaaaggag aaagaaaaaa atgaaaaaaa gaacagcagt aaaaatagta
    19141 gcatcacttt tactcgtagc aatgatagga gcttttatgg tcggttgcgg tactgacaat
    19201 gacaaggtca ccgcttcacc gaagaccacc aagagcgtta gcagcacaag ttcgccggaa
    19261 cccaccaaaa acaccaatcc catagttgta acgtggtatc ctaacgaatc atcggatact
    19321 catgaagctg tgcgtaatga agtcggcagg ttgattgaac aggctaccgg cagaaaagtc
    19381 actcacaaac ttaccaccga ttacacaata accattgaag caattgcaag cggttcagcc
    19441 gatatagcca ttgcaatggg tgctgtcggt tatatagaag caaagaaccg taatccggaa
    19501 attgacgttc tgtttgtcaa cagtgatgaa aattggtcac ttgacggtgc caagtatttt
    19561 gcatggattt gcgtacctgc ggaaaaagcg gatcagtaca aaagcggtga tacatattca
    19621 attgataata taaaaggtaa gaagatgtcc tttgtttcca acagttccac atccggtttc
    19681 agagttccca cttcctctat catctctcat tttaagagtg ataacttaaa tgaagataag
    19741 ctgattgaag gcggtccttt ctttaaagaa gtttacttcg gaggttctca ccagggttct
    19801 gccgtaaact tactcagcgg taacgccgat gttgcagctt tctgtgatat agaagtacag
    19861 ccgtatgtag aaatgaaatc cggggatata agcaaggtag gctcggttgt cgttgtaaaa
    19921 aaaggtgccg atgccccgtt tgatcagtat gtaggaagag aattaacaat aatagccgta
    19981 actcccgtgt ttaacggacc taatgtgtat aatcccaaga atttaagcaa agaagaaata
    20041 aaagccatcc aggatctctt ttgctcggaa gaagttgcaa gtaacaagat actgttttat
    20101 gacgataaag tcgaaggcgc aaaaggattg tacaaaaaat ccaagagtta cggatttgtc
    20161 ctgaccaccg attcatggta tgacccgtat cgcagatagt tttaaggaca atgataaata
    20221 tgcctatatt ggaatttaaa aatgtaagca aagtttacaa caatactacc aaggcgttaa
    20281 ccgatgtttc attttcagta gatgaaggag agtttgtttc cataataggt ccctcaggtt
    20341 caggtaaatc gaccatcttg cgttgcatca acaggcttgt tgacgtaacg caagggtcta
    20401 ttttttttga cggacatgat atcagtcagg cggacaaaaa ggaaatacgg gaaattcgca
    20461 agaaagcggg tatgatattt cagcactata acctcgtaaa ccgcttaagc gtaattgaaa
    20521 atgttttaca cggacgactg ggatataaat cgtcaatagt cggagccgta ggccattata
    20581 ccgaagaaga aaaggaaagg gcttttcata ttctggcaat gctgggactt acggaacagg
    20641 cctataaacg ttgcgatgaa ttgtccggag ggcaaaagca aagagtgggt attgcccgtt
    20701 cccttatgca ggaaccgaga gtgctcctgt gtgatgaacc catagcatcc cttgacccca
    20761 gttcagccaa ggttattatg gattatttgg aatatattaa tgaaaccatg aaaattacct
    20821 gtattgccaa tttgcatcag gtagatgttg ccatggcata tgccaaaaga attatcggaa
    20881 tcacaggcgg acagatttta tacgacggac cgcccgaaaa actgacgaaa gcacaaattt
    20941 atgaaatata tcaatctgac gaaggcgagt tgattacaga tgcaaagtaa acaagttaac
    21001 aaaaaagtat ataatgacaa aacagaaaac aatgcggaaa gagcaccgag accggagaag
    21061 caaacatcga tatttgtaaa aagaaagcgt tcgtttacat tggtgttttt gggaatcatt
    21121 gcactgtttg ccgtttcggc gcttataacg gaatataatg tgattaccgg ttttgaatcc
    21181 attcccaaag ccgttgtctg gatggcaaaa cagtttattc ccgacgcaaa agcctggtcg
    21241 cgtctgtctt ccatacttaa aaaactggcc gaaaccgccc tggtatcggt agctgtcacc
    21301 gtatgtgccg ccatatgtgc attcttttta agcttattcg ggacaaaaac cactaaagca
    21361 catcctttgg ttgccagaat agtcaggatt attgcggcat ttttcaggaa tgttccggat
    21421 gtagtttggg caatgctttt gcttttctct ttcgggcaga atatactgac gggatttttc
    21481 gccctgtttt ttgccacctt cggtatgctc accagagcat ttattgaaac gatagatgaa
    21541 gtgagtgcat cctgtgttga agcattatat gccaccggtg ctacctcttt gcaaacggta
    21601 tttaacggag taattccttc atccatgccg gaaattgttt catgggtctt atacatgatt
    21661 gaaacaaata tccgttcctc cacattgata ggtatgctta cggcaacagg tatcggatat
    21721 ctgtttgact tatactacaa acgaatggat tacggttcgg caagtcttgt ggtgatagcc
    21781 atagttatac tggttatcgc tatagagata atatccaaca ggataagaaa ggttattatg
    21841 taatgagtga acttaatatg acggaaaaac ttcctgtcta tattgatata aaggagtaca
    21901 tgcgaaagac tcgaagcgga aagataaaaa ccacggcaaa atccaagggc ggtattgcta
    21961 taagaataac catatttgca ctggtggcac ttaccgtata cggatttttg acatttgatt
    22021 ataaagatat tgacatggct aaagccgttg ccggcacctg gaacaatgcc aaagtactgt
    22081 tcggtcaacc taaactgaca tataacacat tagcgggagc attaaaagaa ctgcttgtga
    22141 cattttctct gggtgcactt tccaccatct tcggtgcaat aatagctttt ttcggagctc
    22201 tgctatgtgc aaagaatatt gccaatccta ttgtatccgg tatcacaaaa agctttgttg
    22261 cttttataag ggcggtacct acaatactgt gggtcctcat attcaccgtt gcagcaggac
    22321 ttggcagtgt agctgctgtc ataggcttga catttcacag ttgcggttat ttaattaaag
    22381 cgtatgcgga atccattgaa gaggtggatg caggcacaat tgaagcatta aaagcaagcg
    22441 gagcaagttt ctggcaaatt gtatttcagg caatgcttcc ctcctctata agtcgcatga
    22501 cggcatggac attcttacgt tttgaaatta acttcacaaa tgccattgcc gtaggtgctg
    22561 cagcgggagc gggcggaata ggattcgatt tatacatggc aggcagccac tattttgatc
    22621 ttcgcgagct gggattcatc acatacatag tatttgcagc ggttattttg ctggaaatac
    22681 tggcaaccgg aattaaagcg aaggtgaaat gacgatgcaa caggtttttt acgatttttc
    22741 aatttttaat atattaaaag gtaaaaaacc gctgttggtg tgtgacaaaa tatttgacac
    22801 attcggcata aaatttcctt gcaaagttat gcgatttgac gggttcagtt ccaaccccgt
    22861 ttatgaagat atatgtacag gtgtagaaat actaaagaaa aataactgcg attttatagt
    22921 ggcagtaggc ggaggaagta caatagatac cgccaaggcg ataaaatact acagcggtgc
    22981 cgattatcct ttaatggccg ttcctaccac ttccggaacc ggcagtgaag caactcattt
    23041 tgcggtagtc tacataaacg gagaaaagca ctccgtttcc gatcaatgtc tgctgcctaa
    23101 gtatgttatt ttacaatcaa atttgcttaa aacactaccc ttgtatcaga aaaaatgcac
    23161 catgctggat gcactctgcc aagcaataga gtcctggtgg tctaaaagag caaccgaaga
    23221 aagtatcagg tactcaaagg aagcgataga tttaattatg aaatatatgg acagttattt
    23281 gaaaaatgaa gattccggca atgcaaaaat gcttttagca tcccatctgg caggcaaggc
    23341 aataaatatt accactacga cggcacccca cgccatgagc tataaactta cttctcttta
    23401 cggaattccg cacggtcatg ccgttgccat ctgccttccc aaagtatgga agcaaatgag
    23461 caactttgat gacattgcta ttgcattagg tcaacaaaat catgaagccg caatatcatt
    23521 ttttgaagag ctgctacaga ggttgaatat tttgccgcct gataatgtaa acaaagagga
    23581 tttggatata cttacaaatt cggtaaacat agaaagactg agtaacaatc cggttcagtt
    23641 tgataaaaaa actattaaag aattgtatag agagattctg gaggtataaa tggataagag
    23701 agtaaaagcg gttatctttg attgggcggg aacaactatt gactacggct gttttgcacc
    23761 gataaaagga tttatagacg gatttaaaag tataggtata gatatttcta atgaaatggc
    23821 aagaaaacct atggggctgt taaaactgga tcatacacgt gctattgcag ccatgctgcc
    23881 tgaacctgtt acggagcaac agattttgag ggcttacgaa acttttgaag aaactctctt
    23941 tgccaattta gagcaacact gtgatgttaa agattatgtc attgaaacgg ttgcctctct
    24001 cagagcacgg ggaatcagga taggttccac tactggctac acctccgaga tgatgaatgg
    24061 cattttacat aaagtttcgg cacaaggcta ctcccccgat tactggatca gtgcagacaa
    24121 aactgctaag ggcagacctt atccgtacat gatctggaac aatctcatgc acttcggtat
    24181 ttcagattcg agagaagcgg taaaagtggg agatacatgt accgatatat tagaagggaa
    24241 aaatgcaaat tgttggacag tgggcattat tatgggttcg tcggagttgg gtcttacccg
    24301 cagagaggtt tcagccttgt caaaaaatga gttggaagag cgaaaagcaa ttgtccgtgc
    24361 ttcgtactac aaggcgggag cggattatat aatcgacgac atgaatgaac ttttggatgt
    24421 aattacggat atagaccgaa agctcaatca aaatgcagca cggaagttat tgacaccggg
    24481 accgctgact acaaggtatt ctgttaagca tgccatgctc acagatcatt gtacctggga
    24541 cgatgagtat aaggctatta caacttcggt aataaaggat attacacata tttcagccaa
    24601 cgatgattat accaccgttc ttttacaagg cagcggcaca tatgcggtag agacaatgat
    24661 taatacactc tgccgaaaag acgagaaaat attattttta gtaaacggcg aatacgggaa
    24721 acgaatgtta accatcgctg acaaatcggg taaagattat acatatctca gttttgatat
    24781 gactcaacct gtaaatgtta aagctgttga aaaagaatta cagaagcaca agcaagacat
    24841 ttccgtagtc gcattcgttc attgcgaaac cacaaccggc attataaatc ctgttgaaga
    24901 attaacaaaa ctttgcaaat catacggtaa aaaagttctt gtggatgcca tgtccagctt
    24961 cggagcttac gatattgata tgccaagtct ggatatcgat gcattggcct caagtgcaaa
    25021 taaatgtttg gaaggattac ccgggttggc atttgtagtg gtaaagaaga cattgttaga
    25081 gacaagttca ggcaactcct cgtcacacag tcttgattta tttgatcaat ataagtgttt
    25141 ctgcgaaaca aacggcaagt tccgtttcac ctcccccacc aacatactgc ttgctttgag
    25201 acaggctatc gatgattata aaatggaggg cggacttaaa gctcggaaaa agcgttatat
    25261 ggaaaatcat aaagttttat tgcaagaact ggaagaactg ggcatcaagt ctattattgc
    25321 accggaaaat caatcgtata tcataacaac ttttgagctc ggtaatctgg acttctttgc
    25381 attgtaccaa tcgcttaagt caaaaggatt tgttatctat cccggcaaac tcacttccag
    25441 acccactttc agattaggaa atatcggaga tgtgtatccc gaagatatgg ttgcgctggc
    25501 cgatgcgata aaggaattat atcggtttta atcggcttgc ataaaaccaa gactttttgc
    25561 agatagagat ataaacagat tgtcgcttgt ttaatgaagt gctgttgttt ataataaagc
    25621 gcaatagaat aaagatataa ggttattctt tacatgcaag tagttacctt accactaaag
    25681 aatctacctc tttactaaaa tgataagcat ggaccgttta tttacatact tattattttg
    25741 catcagcatt aagagcaatt atttacctca taggtaaatt ttgcattgac aataggtgaa
    25801 aaaaagttat aatggaatta acctcacagg ttaatgctac cataaaaagg aggctaaagt
    25861 tgcagattaa atatagaacg aaagctattg gaagaatctg cacaaatgct tctgtagctg
    25921 tgaaaaaata tgggattgaa atggcggaaa gaatacatct tcgtattgac cagataaaag
    25981 cggcggaaag tgttgagttt atgattaagt acagaatcgg tagatgtcac atgctccgag
    26041 gtaatcgaag cggtcagtat gcagtagatt tagtaaatcc taacaggttg gtatttgaaa
    26101 taacaggtga tgaaatacag attgtaaaca ttattgaaat aattgattat cattaaattt
    26161 aattggaggg ctttcaatga ttaagagcag aacatatatt gctataccgc cgggtacaac
    26221 tatcaaggag cagcttaccg atcgcaaaat gagccaaaag gaatttgcgt caagaatgga
    26281 catgtccgaa aaacatataa gcagacttat caacggtgag gttcagctta cttgtgatgt
    26341 tgcaatgcgt ttagaatcgg tactgggtct tcctgcgcac ttttggaata atctggagat
    26401 aatttacaga gaaaagttaa ttaaagcaca agaagaaaat gaaatggacg cagatctcga
    26461 aatagtaaaa aaatttccat ataatgaaat ggctaaaaac ggatgggttg aagttacgta
    26521 caatccaaaa gaaaaggttt tcaatttaag aaagtttttt gaagttgtta atttgggttt
    26581 ggtaagcgga catcttatac cacgcatagt gtgcagaaaa cttggagatg gagataaaag
    26641 tgactacgca cttatttcgt ggtcgcagaa agcaaaactt gagagtcgca gcttatctgt
    26701 gaggtcagta aacattgaaa aactgaaaac attacttccc gatattagaa aaatgactac
    26761 aaaaaatcct tccgactttt gcccgattct tcgcaaagaa cttgcagact gcgggatagc
    26821 tcttacattc ttaccacata taggcggatc atttttacac ggtgcaacat tttatgacgg
    26881 tggcaaaata gttttaggta tgacagtcag aggaaaagat gccgacagat tttggttcag
    26941 tttattccat gaaatagggc acattgttct tgagcatatc aatcaaactg agggaacaac
    27001 tgatgaagat gaaaaagctg cagatgcgtt tgcacgtaac ttacttattc ccgatgaaca
    27061 atttgatgca tttacattaa ataaagattt tagcaaaacc gcaattatta cttttgcaaa
    27121 taaaatggaa attgatgccg gcattgtatt gggaagactg caaaaggaac aatttattcc
    27181 attcagtcgg catagcgagt taaaaacaaa atacgaattg acagcttaaa accgttattc
    27241 aaaagtcccg cttcgttaaa atagacaaca attttaacct gacttccatt gtacgagtgg
    27301 ctataataat attatttatt gttcgataac aagtcatcaa acagcagtaa gtcttttctg
    27361 ctttgcgcac agcttttaag ctcatcggta aaaccggctg cactgaataa gatataataa
    27421 atgctgtatg cggagctttc cggtactgca gttctggctt tttcctcaag tacccttaat
    27481 atattcatgc cgactttatc cttccaaaat ttacattcac ctaaaattat ctttttttca
    27541 tcaaagttta ttgccgcaat atctatctcg gttttggaat tccaccaaga acctacttta
    27601 gaaaccctga agggtagctt gccgttgtct gaaagatccc acattttctc acggcagaca
    27661 tcttcgtaaa cataggaaac atgagaggtg acgagattct ttcttatcag ttgcatgaca
    27721 aattcggtgt ttcctctttc gatataacta cgattgggat agacaaacaa aaaccaaaaa
    27781 cgtatgaaat tatccttaat tctataaagt ccttttttgc ttctttcagg atttttttcg
    27841 gtaataggta tcttcctctc aataatatcc aaatcaataa gggtcttaag atattttgta
    27901 agacttgtag ccttgacttc aagaaaagag gcaatttccg atattttagt cttccccgcg
    27961 gcaatggctc tgattattgt aaaataactt cctatctcgg acacctcctg tcgaagcaga
    28021 aactgcggct cttcatatag aaactttgat gtacccaaga cattgttctt tatagccgaa
    28081 tatatatcat ccgcttcaga aaaaagctct atatactttg gaacaccacc tgttatagca
    28141 tacatttcaa tgagttcatg cctttcttta tcggggaaaa attcatgata atgtttgaac
    28201 ggaatttgtg tcaaccgaat ctgactcgtt cttcttccgt aaagaggact gttataatta
    28261 agaacctgtg actctatcat tgaaacaaga gagccgcaaa gaataagcat aacctctttg
    28321 tcttttaaaa tctcttccca aatacgttgt aagactgacg ggaatgccga attggactta
    28381 cctatatatt ggaattcatc cataacgata acgggtttac tttcataatc tgcagtcata
    28441 attgctttaa aaatagtatc ccaacttttt atatctgcgt ccctgagtaa ctcattttga
    28501 gtgaattcgg cagccttttt ttgaaaaata agcatatttt gccattccga ttcttcactt
    28561 gccagaaaaa atagagcatt tttatcttta ataaaatttg agattagagt tgttttacct
    28621 accctgcgtc ttccgtatat tacgacaaat gaactgccct ttctattata ttcaccctca
    28681 agttcctcaa tttccgcata gcggtttata aatctcttca tatggaatat tcacaccttt
    28741 caatataact acgatatctg ttaagcatac taaacatacc gcaaagaata aaatgattac
    28801 tgcaatcact acattcaaaa ttattatact tcaaattata ataaaatcaa gtataatatt
    28861 tttcaaactc taaatacgag aattttttta aaaaaaggtt tgcaatatat tgcaaagaag
    28921 aatacacaca aaacatcatg ggtgtcacaa attatgttat aattaacata acaaatcaaa
    28981 atttgcatag gacatgattg aatatgaaac ttatacttga agatgaatcc atatccgcca
    29041 aaaacatgag aaattgtcat aaaataatgc tggatatttt cggtgatgat gtcgagctcg
    29101 ttgattattg gggacctgtt cagatgtgcg tcttttatct ggaatacaac tattctcccg
    29161 atgattataa aataataatg gaatgtgaac gcggatttat tgttatagaa gtacaggata
    29221 aagccggtaa tgttttctgg ccgtttatga tattccccca ggccaattat tttcattatg
    29281 cagatgtcga aaaggatgtt ctgcaacttg tagagttgac tcataaggcg ataaaagaga
    29341 atctgattat ttttgaaccg tccggtacaa caaaatattc cgattcgaca tgtttacatt
    29401 gttttgatgt cagggaagtt cacaacttta tttcacctga acggtatgaa gaaaccatag
    29461 aatatattaa agaactgatt gaaaaagaaa actttatcct ggtagaaggt aactgtccta
    29521 taggagaaca taagaaagac ggatattgga ttgatgatat catttatcat gttataaaat
    29581 gtccgaaatg cggctggaaa ttcacctgca ttgtaaacac ctacagagga agcggttcat
    29641 ttacaagaga aagataaatt catgttttta aggcgacatt tgcatcgttg ctttttaatg
    29701 cattgataca cagggttttt aagaagataa agaaactata aggcaacaga taaactgtac
    29761 cgggtgccgt atcttctttg gtgcagatgg cgatacagga cttttttata aaatcccatg
    29821 aaatcaatat ttgcaggcag tcaataggca ggatattcct taaatagttc cctattctct
    29881 caaaataaat cgctatgaga tcccgttctg ctcaaaatca agatgagctc tttccgttta
    29941 attttataga taagaagcca atcgggagtt atatgcaaat ctcggtaacc tttaagattg
    30001 cctgtaagtg catgatctcg ataatgttca ggtaaaggcg tatttttcgc aagcaaacta
    30061 attgcctcgt ggagtaaatc catattcatt ccacgctttt ttaaccgttt atagtcgcgc
    30121 ttaaatgaac tgtgatattt aatcttcagc atttaaactt tccaccaatt cttcaattgt
    30181 ttcaaaaggt ccatgcaaat ttttaccttc ttcaacatcg cggatagcag atagcgtttg
    30241 gctgttgaga ttggaaagtg agagctcaaa aggtattctt tgctcgcgta cagctgcctt
    30301 agtaaacatt gttaaagccg tagtcatatt taagccgagt tcattaaaca gtttctcggc
    30361 ttcctgctta agttcgcgat ccattcttat tgttatattg gtctggccca tagtttatca
    30421 cctccgttac aaaagtatta tacattatat tactgtgcaa ttcaagtgcg atacatgtgc
    30481 gtgatagtaa atatttgcaa atcctttcac tcgcaataag acaagtgttc ttacagaatt
    30541 taattcatta aattcgctga caaaacttgc aaaatgaaga aaagaaaagg aaaggaaagc
    30601 agtcagatgt agctgctgct ttccaatgaa tggtgcggat gacaggactt gaacctgcac
    30661 acttccgtat tggctcctaa gaccaacgcg tctgccaatt ccgccacatc cgcagcatag
    30721 aaaagtatat ttaatgaaca catctttgtc aatcctcgcc ggaaacagtg atatattcct
    30781 taattttctc aaacttggca ttttttatac ccggcacctt cattatatcc tcaataacct
    30841 taaaattacc gttcttaata cgataagcga ttatcgcctg agcagtctta ctgcctatac
    30901 ccggaagcag acataaagtc tcttctgaag aggtattaat atcaatcttg ttctcaaaaa
    30961 gcaccttttc atcatctttt tgtattatga catcgctgtt atccgatata tctattcctc
    31021 ccgcccggct tttcggtcgt atgtaaacgg tcatgttttt atccagcacc ataaccatat
    31081 taatatcttg gcccgcatcc tctgtcaaac ctccggcttt gtctataagg tcggtaagaa
    31141 tactgccttt ttccacttca tatattcccg gattatttat ttctccctta agacatattt
    31201 taatggtctc cttggtagaa gctgttgaaa gctcatgttc gtcgggaata tccacaaatc
    31261 ccctgtttat atttctgaca atgagaaata taatcagaaa aacgaagcat acggtaatga
    31321 taattacggg tgtgtacttt ttcatagata cttactatca catcggttga taagttacca
    31381 ctgacaaaat caattctttt cattcaaaac aaatatcata tgtagtataa tttacaaaag
    31441 aaaggtaatc atgagaaaaa gcatttcaat aatacttgta ttaacaatag cagcatgcct
    31501 tttcaacatt aatgtacaag gtaacgctca aagcataatc aatcccgaat tatgtcgttc
    31561 atatatatcc gttgattatg agacgggaga tgtcttgtac tcatataatg ccgacaagag
    31621 gatttatccc gcaagtacca caaaactcat gacagccctt gtagcggtgg aacactgttc
    31681 tttggataaa gtttttacta cctcgctgaa tgcaaccaga tatcttgagg aaggaagcac
    31741 cgaactggaa ctcaaagaga atgaaacaat ggatttgtat tccctgctcc acgcattgtt
    31801 gataagaagt tataatgatg tggcactggt aatagcggaa aatgttgcag gtacagaaga
    31861 agcattcatt gaaatgatga ataaaaaagc tttggaatta ggtgcttcaa atacacattt
    31921 tgtgacacct cacggacttc atgatgatgc tcactataca acggcaagag atatggcact
    31981 gatagcaaaa gcggcttttt ccaatttcaa catatccaca atatgcagaa aaaaagagtt
    32041 caatcttccc gcaacaaact taagacagaa gtttgaaacg cttaagaata caaaccttat
    32101 actgggcaca aatacgggat atgattttgt agtaaccgcc ggaaaaaccg gatacacatc
    32161 aaaagcacag aatttacttg tagcattatc caccgatcag aaaggacggg aagttcttac
    32221 ggtaatggca ggaacaaaag aaagagaaac cgctacggaa ctgactttgg agcttatgaa
    32281 atattcttat acggatttcg gcgtccaaag tattgtcaaa gccggacaga taataggaac
    32341 gcatgaggac atccgtttag ccaatgccat gtacatagaa cacttagtac cgtatgacac
    32401 cgattcctgg caattggaca agacaatcga attgagagat gatatcatat atacgcaagt
    32461 aagagtcggt gacatcttgg gtgaagtttc ctattcatat aataacaatt atataggcaa
    32521 atcactcatt gtagctgccg attctcttat tccggaagtg acttccgtta agatttcttc
    32581 ggaagatccg ccaaaggatg ttgcggtaac ggcaatggtg gaaaggcaac cctttccccc
    32641 ggcgtttatt gcggcattaa gcataagtgt gattttactg gttcttatca tagttgtatt
    32701 ttataaaaca agtaaacgca ctgttgataa gacaaaggaa aaggaagata ataatgataa
    32761 agatacaaaa gataacggat aatgcgggag gggtttatga agcagctttt tgatacgata
    32821 gcacaggcgg aaatgctggg atttgcattt ttggtgatag ccgcaatggt tatatgtgtg
    32881 gcgattattt tcttttttgc acttataata gttctggaca aaaaaagtat tcccgtactg
    32941 agaccggcaa gagaactgga tatactgaaa aaacatatgt tggatatcaa agccaacttc
    33001 ccggcatatg taaacaatat acttactctt gatgaaggca aggatttgaa aaaaagcaat
    33061 gaaccgctgg atataggggt gataatactg aaaaactacg gcaacagttc cgcagtggat
    33121 attcaggtta cacatatcgg cagttacaaa gtagaggaag atttctccaa taaatatgtc
    33181 tcccttgagc ccggtgaaag cgttgccgtt ctggtctata ttcccaaaga cttattaagt
    33241 tcggtcaaag tatcgcttgt caaagtgaaa tcgaaaaatt acgccaacag taccagaacg
    33301 gaaaaattca gtataatatc cgacagcgaa ttgagctacc ggataagtga aaaatgtact
    33361 ttacatcctt ttattcagat gtaatgcatt aaagtttata tatcttcttt aaatcttctg
    33421 atatttcttt cagcgaagca tatatcaagt cgggttttgc gtcgctgttt tttacatcct
    33481 ccaaagaaga ctccccggaa agaacaagta tcgatgtaaa accgttgtag gctcccaagg
    33541 ctatatcggt gtatactctg tcccctacta ttgccagtga ttttttatcg tatccggtct
    33601 tttcaaaaat gaaatccgcc gtatgttttg acggttttcc gaaaaattcc gcacttacac
    33661 ccgtagacgc atataccaac tgacatatgg aaccgcagtc gggaatgaac ccggtttcgg
    33721 taggacagtt gtagtccata ttgacaccgt ataaagttgc accgtaacgc acgaaatcgc
    33781 aggctttgac aagcttttca tatgtcagcg ttgtatcaaa tcccataaca accacatcgc
    33841 aatccgtatc cgttacatta attccggctt cctcaaaatc atcgataaga tactgtgtac
    33901 cggcaacata tactctgctt ttttgatgat gctccagaag atgctttata attacctgat
    33961 tggagataag catctttctc tcttcacaaa caatccccat tttcttcagt ttattgatat
    34021 agaacttagc gttctttgag gaattgttcg tgtagtacca tatatctatt ccgcattgct
    34081 ccgccgtggc taaaaactct tttgtataat caaaaagttg atttcccaga taaatagttc
    34141 cgtccatatc caatacaaaa cattttatgt cttttaacgc cattgcatct cccctatctt
    34201 atcgccttta tcttgcaaac cattattatc tttcggataa actgattata cagcaaatgt
    34261 gcttttatta ccttaaagaa caactttatc cgcatttttc ttgtctcatg ccgatagttt
    34321 ttagatatat tgccgccttt ttcaaaactt tttgctaacg cacatgcact gtaaaaagca
    34381 aaactgaatc cttctgcgga actgggcgac acataaccgg ccgcttcacc tgcaagaaac
    34441 aatgtattgt tgttttttga aaatgttttc ggatagccgt ccggtcgtaa caacagtgag
    34501 ccttccttat ttacagcctg acctattgat aacccgtcat tcttcatttt ttcaataagt
    34561 tgagcaaaca gtctgttacc gtccgctccg tcgggaaatg ccatacctat agtaaaagta
    34621 ccgtctttgg ggaatgccca ccctgaataa tcggtcaatg ttttgtcaaa tattgcataa
    34681 tatttagcac cggcatcgtc acatttatac acatgctgaa tagcataata atgcttgaaa
    34741 ggcatttttt cttttgtaaa aaagcacttt ctcacaaccg atccggctcc gtcacaacct
    34801 attaaaacct ttgaattata tgtcttcccg gtacctgtat gtatagtata caggtcattt
    34861 tcgtactcaa ccttattgac cactgcggag tattctttgc atacattttc cggtattttt
    34921 gaagacagat accggtcgaa tttttctctg tccatgttga gatagaatct ctgataatct
    34981 cttcttaaac cggtattcaa gtctacggta tgcacactga aaagctgcgg attagccagc
    35041 acatcattgg gaaggttaag attcaaagaa gctaaaaccc tttgtgcatc gggtgcaaga
    35101 agccctccgc aggccttgtg ccttacggga ggataaccgt caagatttct cttgtctata
    35161 agtaatatgt catgaccctg atttgccaac agcatagtaa gcaggcttcc ggcaggaccc
    35221 gctcctatta tcgctatatc aaatatcttc ttctcatcca ttacacaaca atgtttataa
    35281 gtctcttggg caccgctata accttcttag gttgcttccc ttccagaaca tttaatattt
    35341 cttgattttt atataccagt tgttccatat cttctctgga catatcggaa cttatgaata
    35401 ttttagtctt gtttctgccg ttaatctgaa tgactatttc tatctcgtct tcaacggttt
    35461 tggcctcatc atattgcggc catttctgtt gatgaatata cccgggaaat ccgcataatg
    35521 accatagctc ttcggtaaca tgaggagaaa cggggttaag aagtataagg aagtctgcaa
    35581 actctttttt ggttatactt cccttagcat aaattgcatt ggtaaatgcc atcaactgtg
    35641 caatggcggt gttgaatttc attatttcaa aatcttttgt aaccttttta atggtctgat
    35701 gcatcagcgt ctccatatcc ttggagtagt tgtcctcttc ggtcatgaaa tccgtcatct
    35761 taaaagctct ttcaataaat cgcctgcaac ctttaacgcc gttttccgac catggagcgc
    35821 ttttttcaaa atcccctatg aacatctcat acatacgcaa ggtatcggca ccgtaatcat
    35881 ctattacctt atcgggatta actacattac cgcgcgattt actcatcttt tcattgtttt
    35941 cacccagtat catgccgtgt gcggttcttt tactgtacgg ctcgggagta ttgacataac
    36001 ctatgtcgta aagaaactta tgccagaatc tggagtaaag cagatgcaac gtaacatgct
    36061 ccattccccc gttataccag tccacaggca tccagtatgc tattttttct ttggatgcga
    36121 atgcttcatc gttatgagga tcaatatacc ttaaaaagta ccaggaagat cctgcccagt
    36181 tgggcatggt gtcggtttcc cgtttagcag gctttccgca tttcggacag gttgtattta
    36241 cccaatcggt aatcttggaa agcggcgact ccccgtcttc cgcaggttca tatgtttcca
    36301 aatcgggcaa caacaaaggt aactccgatt cttcaatagg tacccaaccg catgtgtcgc
    36361 aataaaccag cggtatcggt tctccccagt atcgctgtct ggaaaatacc cagtcacgga
    36421 gcttgaaatt gtcctttgct tttcctattc cttttgattc aatatattca attatcttaa
    36481 ctttggcgtc ttttacttcc aatccgtcga tgatacccga attgaccatt actccttctt
    36541 caatatcggt gtatgcttcc accgataaat ctcctcccga taccacttca attatgggca
    36601 gattgaactt ctttgcgaac tcatagtctc ttttgtcatg tccgggaaca gccattatcg
    36661 ctccggtacc gtaggacatc agtacataat ccgataccca tatggatatc tctttgttgt
    36721 taacgggatt tacggctttt atacctttta tctccactcc ggttttttcc ttggctatct
    36781 ccgatctttc aaaatcactc ttctttgaac tttcggtctg atattcacgg atttcatcaa
    36841 tattttcaat taataattta tattcctcta aatacggatg ttccggagat attaccatat
    36901 atgtcactcc gtacaaagta tccggccttg tagtatacac aacaagaggt tcggaaatat
    36961 ttgtcaattt gaaatatact tcggctcctg tggatttacc tatccagtta atctgctgtg
    37021 cttttatact ttccagataa tcgagatcgg taagaccttc aataagttta tcggcatagt
    37081 cggtaatctt aagcatccac tgattcttca tcttctttac aacttccgct ccgcaccttt
    37141 cgcatctgcc gtccaccact tcttcattgg caagacctac tttacaggaa gtacagtagt
    37201 tgatactcat attcttttta tatgcaagtc cgtgtttgaa taattgcaga aaaatccact
    37261 gcgtccatct gtaatactta ggatcggttg tactgatttc cctgtcccag tcaaatgaat
    37321 atcctatgga tttaagctgt tttttaaatc ttgctatatt gtttttggtt acagccgcag
    37381 gatgcaactt atgcttcatg gcataatttt ccgtaggtaa tccgaatgca tcccatccca
    37441 tgggaaacag cacattatat ccttgcagcc ttctttttct ggcaattata tccaatgcgg
    37501 tatacggtct gggatgacct acatgcagtc cctcgcctga aggataggga aactccacca
    37561 aggcataata cttgggtagt gaaaaatcgt cctttgcatt aaaacacttt aaatcgtccc
    37621 aaatcttctg ccactttgcc tcaatttctt taggattata ttccataaca atactctccg
    37681 aaaccaatat tcttcacagt attttattgt aaatactgta cattgtcaag aaaaggtata
    37741 tctgttgaac agagcaaaat actctttata aagaacgatt atctaacatt tttaagtaag
    37801 atttttctaa atatgccact ccgacattac ataatacagc ctctattgca accaagcatt
    37861 ggctttaatg aataaaattc attaaatcag caagcgaact cagagtattg tctaactttt
    37921 ttataatcct ttatatgtga ttttccgaca aaagttggat taaatatgct taataatgct
    37981 attttgttaa tttatacgaa atttatatca ataatatttt atttttattt aacatattgt
    38041 tagtcacttt acttgactgg tatgaaaaaa agtgtataat ggatatgagt tttgaatatt
    38101 tattcaaggg aggaaaatca atgaacaaaa tgaaactgat tagtttggta ctttgcttgg
    38161 tgcttgtttg ctcgtttgtt ttcacggctt gcgacaaaaa gcctactgaa actgcaacac
    38221 agcaaccgac agagacgagt actcagactg aaacacccac ggaaacacaa atcgatacca
    38281 cacctgcagt ggtagagaaa ccggcggtaa tggatataac taccctttac tattacggtg
    38341 atgcaacgga aaatgcagct gagaaattag cgtataaaaa ctacctgaac agacattacg
    38401 gtttcacaat caacattcat gctcatgcca gagacatgta cattgaaact atcaatcttc
    38461 gagctgtatc gggtgatctt accggtatag taagactttt cgcaggaacc gacggaatca
    38521 aatggtatca ggaaggtatt gtgctggcaa ttgatgaatt tcttgccgat aatgaagttt
    38581 ggaacactat tgttccggag cattggaaag aaactttctc atatgacgga gcagtatacg
    38641 gacttgcatt cggcggtgac ggtactcctt catggttttc aagaacaatg agaggtgact
    38701 ggttggatgc ggtaggaatg tccagacctt ccaccataga tgagttctat gaggtatcaa
    38761 aagcctttac ctacaatgac cccgacaaaa acggagataa cgacacttgg ggattctgct
    38821 caagacatgt atggctgatg caggatattt tcaaggcata cggttgtaca cccaattggg
    38881 aatccgcact ggttcctgta tggaatccga gagatgatat atgggaagat ccggttataa
    38941 agccggtcat ggctgatgca tgtacattct taagaaagtg ctatgtagaa ggcattatac
    39001 atcccgagtt attctcaatg agttccacca atgtaaggaa tcttatgtcc aacggacatg
    39061 caggctcctg tttttactgg gatcactggt tgatagtatg ggaaaatgcg gtaaagaaaa
    39121 cagtaggaag ccatgcttat atggtagctg tcggtgctct ttcaggtgtt gttacacaca
    39181 agataaattg ctggaagacg gaaatcggat ctccttatgt actgatggct aatacagcac
    39241 aacccaagga agttataaat tggttcatta acttggctta cggtaccgcc gaagacttct
    39301 ttacattcag atatggcata cctcaaactg ataagaaagc aaaaaccggt ttcctgcttg
    39361 acggaatgac cgtatatctg ttgcacttca aagctgatga ccaaggttcg gtcacaagtt
    39421 ccggctgccc tgctttggtc ggcggacacc ccggatatgc cctggatatc ggcgagtacg
    39481 gatataaatc cgcatactat tccggaaacc cgacatggga ttcggaacag gtaacagtaa
    39541 ctgccgcaaa ccttcaaaga agatttgaag tatttaacga attcaatgac ggcagattgt
    39601 atctgcttac aggcgactat gagcagccta caaatcctga atacacgaat aatatgactg
    39661 aatggaataa ggcaggcaga ttgtatgtat ccagcgtaat ggttgacggt gtagatacaa
    39721 cgactgctct taagacttat cttgatacgg ttatggcttt cgatccgcaa accacattgg
    39781 atttcatgaa tgctaaatta ggaaaaactt ccaatcagga ttatagaaag ttatggaaca
    39841 gcctcaactg attataagat aaagtaaccg caatcaaaga gcccgggaaa ccgggttctt
    39901 tttcctttaa taaattaatt tctcctctct tgccgactct aatttattat ttgcttaaat
    39961 gcagttatct tattacaaat taactgtcta acattgaaaa atattaaatt tgcgacagta
    40021 tgttattaaa agattacata atagcatcca taatgcaatt atcagtttac tttagtgcaa
    40081 aattacattt attgaactca aatcggtttt aaagagacta actttttcaa cctattcaac
    40141 tttggaattt tatgacatat tgagcaaaaa acatcataaa gccatacttc ccctatatta
    40201 ttaacatttt ttgttagtta ttttttaccc atttgttaga tgtatgttat tgacttgtat
    40261 ggtgtttgtt atatataatg gacatatgtt ttgacttata ttcaaaagga ggagaaatta
    40321 taaaaaagat taaattactt agtttggtac tttgtctcgt tctggtttgc tcgctagttt
    40381 tcacggcttg tgataagaag cctaccgaaa ctgcaacgca aaaacctaca gagacatcta
    40441 ctcaaaccga acaacctacc gaaacggcta ctgcacctgc agaagtggaa aaaccggcgg
    40501 tattggatat tacaaccctg tattactacg gtgatgcaac ggagaattcc gccgagaaac
    40561 tcggatttaa gaactacctg tgcagacact acggtatcca gttcaatatt catgcccctg
    40621 caagagacat gtacattgaa accattaatc tcagagcggt atcgggagaa cttaccggaa
    40681 tagtaagact tttctccgga accgacggca ttaaatggta tcaagacggt gttgtattgg
    40741 caattgatga atatctcaag gataacgaag tttggaatac cattattccc gagcactgga
    40801 aagagacttt ctcttatgac ggaaaagtat acggattggc attcggcggt gacggaacac
    40861 cttcatggtt ctccagaaca atgagaggcg actggctgga tgctgtagga atgaaaaagc
    40921 cttctaccat cgaagagttc tacgaagtat ccaaagcctt cacctacaat gaccctgaca
    40981 aaaacggtga taacgatact tggggatttg cttccagaaa tatctggctt atgcaggata
    41041 ttttcaaagc atacggttgt acacctaact gggaagccga tcttgttccg gtatggaacc
    41101 ccagagacga catttgggaa gatccggtta taaagcccgt tatggctgaa gcatgcttat
    41161 tcttaagaaa gtgctacaac gaaggactgg ttcatcccga gttattctcc atgagctcga
    41221 caaatgtcag aaaccttatc tccaacggac atgcaggctc ctgtttctat tgggatggat
    41281 ggttgattac atgggagaat gccgtaaaga aaactgcagg aaaccaggct tatatggtag
    41341 catgcggtgc actttccggt tatgtaactc agaagataaa ttgctggaag acggaaatcg
    41401 gatctcctta tgtactgatg gccaatacac agcagcccaa ggaagttatt aactggttca
    41461 ttaactgggc atacggttct gcggaaggtt tcttcacatt cagatacggt gtacctcaga
    41521 cagacaaaaa tgcaaaaacc ggtttcatgc ttgacggcat gaacgtatat cagcttcatt
    41581 acaaagccga taatgaaggt aatgtaaaga gcacctcatg cccggctata gtcggcggac
    41641 accccggata tgctctggat atcggtgaat acggatacaa atcggcatac tattccggaa
    41701 acccgcaatg ggatgctgac caggtacaga taaatgccgc aaatcttaag agaagatacg
    41761 atgtgttcca cgagtacaat gacggcagat tatacatact tacgggtgaa tacgaacagc
    41821 ccacaaatcc ggaatatacc gatcatataa gcgaatgggc aaaagcagga atgctgtatg
    41881 tatccagcgt aatggttgac caagttgaac ccaaagtagc tctcaaggct tatcttgaga
    41941 cggttatggc gttcaatcct caggccacat tggacttcat gaatgctaaa ttaggaaaga
    42001 cttccaatca gaattataat gaggtatgga acagcctgaa ctgatttatc aagtaaaaaa
    42061 attagaactg aagaacccgg taacccgggt tctttttttg cctttacatc tttagctttt
    42121 atcagcatta tctttatgct atacaatagc agatgctgat tagtaaccgt actgctcttt
    42181 gaattgattc aattgatcca ataactccgg aagtctgtgt cccataaaca ctacaaacac
    42241 tatcaggaat acaataaaag caatcacggc tgtagccata agaataatct gggctttgaa
    42301 taaatttttc ttactttcct tttctccggc aaaagcaaac acaaagagca ttattatatt
    42361 gacgacaggt atggacatta caataatagt taatatccag tccagtatgg aaatagtctt
    42421 tgatgtgtcc gatttggtaa cagcaggctg ttgatagacc ggttgctgat aggcttcctg
    42481 ctgatacgtt tgttgctgat aagtttcctg ctgatattcc tgttgctcat tatcctgctg
    42541 cacatcatca acaatggttt cttctaccac attattgttc tccatttccg cagtttcctg
    42601 aatgtcctgg ttattctgtt gattattagt ttcagtcatt tttaatccct ccttttcaag
    42661 ttaaattata ttatgagatg aaatattgtc aataaaacaa tcttataaag tgcaataata
    42721 ctgcattgtt gtatatggtt aacggttcgg taccgaatga tgtggctgcc cttacaacaa
    42781 atatgacaaa caggagcgac aaagtcacgg ttgccaaaag cttaatctgt tttacgttgc
    42841 tgttaagaaa gtatctcatg acgataaata taatgaaagc aattaatata acatatatcc
    42901 cgggatgcat aataaaggct tctttgaacc tgaatgtgat aagcagctca aaagctctca
    42961 ccattccgca tccggcacac ggtactccga aaagtaattt cgacggacat acctctccta
    43021 ccgtaagcgt cattattacc acatatgcta tacatgccgt aacgggaatc catgcttttt
    43081 ttatatcctt aagcaatcgc tcccacactt ttctcaacat atcagtatcc caattcctcg
    43141 ttaactatta ttaccgttat tcttccctgc tcacgttgtt ttgccgatat tacatagttg
    43201 cagcatatca tgtcggctgt gttacaggtt ttgttaatat catcggatat gcattttccc
    43261 gtacgggcac agggggtatt cctgcttaat ctgatacagt ttttcggtgc cgcaatgtgt
    43321 tttaccctca aaacagcctg ttgtatatcc ttaactattt tatttacacc ggcaactatt
    43381 attacctttt taggcccgtg acatatacaa gccactctgt tgctgtaacc gtcgacattg
    43441 tacagtttac cgtcttcggt tatggcattt gtgccggaaa gaatgaaatc cgcactgaac
    43501 atggcagctt tatcgcctct ttcaactaag cgataccggt cttcacgaat catttttgtt
    43561 attccgcatt ggtcgagagt aaccgatccg cctgttgtaa tcgaagcatc ttcgaaaagc
    43621 atttctttaa ccaactctct ggcacaatct ctgttttccg caaaaacagc attcatgttg
    43681 ttcttcctca aattgtccaa tactttgttt atatccactt ctcttctcct ttctacttta
    43741 attatatcat ttacaacagt aattcatcaa agaaccctga gttcttttaa accaatactg
    43801 actcttttgg aacttttata gaacttttac agattcctgc ccgtctaaat gctgtaagtt
    43861 aaaaaaggac ggtatataaa aatgaaaaaa ttgatagctg ttttgttaat tctcatatta
    43921 ctgctgaccg gttgcaccga tgttactcaa ccttcaccgc aaccgtcggc tgttgtgaga
    43981 gatgaaagta ttaaaagcat tgaaagtctt tctcaactca agtcactgct ggatgcatcc
    44041 aaagacaata ttttctccag atggtacaga tcggtaggca gcgctctgtt agccaaggat
    44101 gcaaacggac cggactatgc agtagaatcg gtaacagcga cgggtgatgc gagccaatcg
    44161 cactccgaga ccaatacaca ggtggcggga gtggatgaag cggataccgt aaagaccgac
    44221 ggtaagttta tatattccat cagcagttac ggttttgtaa atatagtctc tgtcgattct
    44281 aacggctatt tggaactggt aaaacaaata aaggatttcg gtaaaaactt cagtcccgct
    44341 gagatatatg ttgatgacca attccttata gtaatcggaa catcctacga catggaaggc
    44401 tcctactcag cagcatacat atatgacaaa accgatatag cggaactcaa attacttaag
    44461 gatgtaatga taaagggtta ctactccgat tccagaaaga taggcaactc tctttattta
    44521 atctgcaact acacccccta ttactattat ttaaaggata cggatcttct gccctgctat
    44581 attgaaaacg gtaaaaccac ataccagtca ccttccgata tcttctattt cgacgagacc
    44641 gaatgttcat catatctgat actgggtgct gttgatttat ccgatcttac aaaggatatt
    44701 aaccttaata cttacttagg aagcggccag accgtatata tgtcccatga aaacctgtat
    44761 gttacctact cacagtatcc ttacaaacct gtcagaaaaa tattacccga agaggactca
    44821 tattattaca aggaagaaac cgtaatacat aaattccgtg cagataatgc agtgctgaat
    44881 tatgttgcca ccgccaagat tacgggatac cccatcaatc agtttgcaat ggatgaatac
    44941 aacggattct tcagaatagc ggttaccgat aatttggaag gcaaatcggt caataacgta
    45001 tatgtttttg atgaaaatct ggttcagaca ggcacaatac agaatatggc acccggtgaa
    45061 aggatatatt ccgtaagatt taacggtgat atcggttata tggttacatt cgtggtggtt
    45121 gaccctctat tcgtaatgga tctttccgac ccgtacaatc ccaaagtaga cggagaactc
    45181 aagattcccg gatactcgac ctatatgcac atgtattccg atactctcat actgggcttc
    45241 ggaatggata caacggagca ggatggcaga gcattgaatc agggtattaa aatcgcaatg
    45301 tttgatgttt cggacagagc caatcccaaa cagcttttca caacttctat cggcggacgc
    45361 ggcacatggt cggaactgaa ttacaaccat aaggctttta tgtttgaccg tgaaaaagac
    45421 atattcacat tccccgcttc aataagcggc aacaactatg agatgaagac acaaggattg
    45481 gttgtatgta aaatcgatat gcaaaacaac cggtttgaag tactggatat attgtctcag
    45541 gattacttta ccaactggtc caattatgtg gaaagaggta tgtatatcgg gaacaccctg
    45601 ttaacaatct ctaaaaatgc catctactcg tataactaca ctacattaca gtttattgac
    45661 aatgtccttt ttgattgata agcgtttctc ctaatctttt tataccctct ctgtaaatag
    45721 cggcgccttc atacaggcgc tgctttttta tgcactcatt tgcctgatga gcactttcaa
    45781 gatcaccggg catgttcatt ccgaaagcca caagattggg catacttctt gcatatgagc
    45841 ctccgcctat cgctataggt tcggtataat catttgtcat ttccctgtat accgaaagca
    45901 atgttgatac cagtaccgag tttttatcgg catacaacgg agggttatta tccgtataaa
    45961 ccgctcttaa atcatatctg ccgcaaacct tttctatttt ctccacaatt tctttatagt
    46021 cataagttac gggatagcgt atattaagta cgcagacgga tttttccttg tctataacca
    46081 taactccggg gtttaccgta agtcttcctg tctcatcttt acagtcaata tccagtcctt
    46141 ctccgtaatg cttatcggca agtgcatctc tgaaaaactc aataatcggt ataccggtaa
    46201 gaaccggatt gttttcaatg gctatcttaa ttgcgtttat gccgttaaaa ggagccgctc
    46261 cgtgagcagg tttaccttca taaacaaact tttttccttc atactccaca cgacattttc
    46321 ccggaacaac attggggcga tctcccgctt gtaacgatat gtctttgttc ccctctcctc
    46381 ctatagccac cgtgagaatg cctttttcag cataaattac ggggaaatcc gcatccggta
    46441 caaatcccat tgtcggaagt tcctccgtct ttacataatg agccatacag gaagagccgc
    46501 attcctcatc caaaccgaat ataaggcgaa ccctgtaccc ttttatctca ccttttttaa
    46561 taaggtcaac aagagcataa aatgcggcaa ttgccggtcc tttatcgtcc gacactcctc
    46621 tgccgtataa gaagccgtct ctttccgtca acgtgtacgg gtcgcttgtc caaccgtcct
    46681 gctcgggaac cacatccaga tgaagaagaa cacctatcat gtgttttcct tcaccgtatt
    46741 caagatatcc cgctcttccg tcaatattta cagccttcat accgtatttc ttaccgagat
    46801 tcagcatata ctccaaagct ttgagtgtac cttctccgta cggtgcatta ggagcaggct
    46861 catctttaac gctttttatg gctatcagcc ctcttaaatc ttcaatcata tcatccaaat
    46921 attgttccat ttataacatt tctccttaac gtagtaaaca gtaaaatgta tcatcattta
    46981 caagta
//
LOCUS       DAOSOM010000013        46553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_841_length_46553_cov_5.563336, whole genome shotgun sequence.
ACCESSION   DAOSOM010000013 DAOSOM010000000
VERSION     DAOSOM010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 46553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46553
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_841_length_46553_cov_5.563336"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /gene="dnaE"
                     /locus_tag="PLF14_04570"
     CDS             complement(<1..1104)
                     /gene="dnaE"
                     /locus_tag="PLF14_04570"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOT70604.1"
                     /translation="MTDFVHLHVHTEYSLLDGSTRIPDMVSKAKDLGMKAVAITDHGA
                     MYGILQLYKECRKVGIKPIIGCEVYTARRKRTDKIHGIDSTQGHLVLLAKNNTGLHNL
                     MKMVSQSFEDGYYYKPRVDKELLRQYSEGIIALSACLSGEIPEAIIDKDFNKAKATAL
                     EYLDIFGEGNFYLELQYNKIPEQELVNKYLIKLSNELNIPLVATNDVHYLEKTDAKAH
                     DILLCIQTAKTVLDENRMRFPSDEFYFKSQDEMMKEFSNVPEAITNTQKIADMCDVNF
                     NLNEMHLPKFPLPEGIDHYEYMKDIVFEGLKRRYKDKLNEKHVERAKYELKVINDMKY
                     VDYFLITADFIDYAKKNGIPVGPGRGSAAGSIVA"
     gene            complement(1097..1723)
                     /gene="rdgB"
                     /locus_tag="PLF14_04575"
     CDS             complement(1097..1723)
                     /gene="rdgB"
                     /locus_tag="PLF14_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOT70605.1"
                     /translation="MMKKKTIVVASNNKGKIKEIKALLPDIELLSLSDIGVSDTPEET
                     ENTFIKNALIKARHAKAFTEHTVIADDSGLEVFCLDNMPGVFSKRLYVNRGGKTEENM
                     TDSACIDKLNLCTLLKMMEKKECDKRDARFVCVIALINSDRTEVVFEGQCRGRILTEP
                     KGSGGFGYDPVFLPDGYDKTFAEMDDEQKNGISHRANALRKLKEYLDD"
     gene            complement(1720..2430)
                     /gene="rph"
                     /locus_tag="PLF14_04580"
     CDS             complement(1720..2430)
                     /gene="rph"
                     /locus_tag="PLF14_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOT70606.1"
                     /translation="MRIDKRQNDELRPITIKRNVMKYAEGSAFIECGETKVLCTASVS
                     FESPPFREGKGWVTAEYNMLPRATQQRNKRDISSLKKNSRASEIERLIGRSLRACCDL
                     DAFNDVTIIIDCDVIQADGGTRTASITAGYVALYDAFEWMKEKGYVEKNPVICQIAAI
                     SAGIVHNEVMVDLCYAEDSNAQVDVNVVTDSFGNIAEIQATGEKSTFDKEQLDEIIAL
                     SQKCIERIMEIQRKCLAE"
     gene            complement(2541..4079)
                     /locus_tag="PLF14_04585"
     CDS             complement(2541..4079)
                     /locus_tag="PLF14_04585"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOT70607.1"
                     /translation="MEFDYKDSGMKKKIITIAVIMVILCIVIAVVIFILTNEDLINNN
                     PKASSTPIIQQTETQGPDVQSPQPTEIRQPKEIKISYENEVLIFEGLSKDCEFHTVFE
                     NNKDYIFNELRHPDDTKKRSWYEINNKSTIYFTVLLDEFNLYNYQDQSLFPDNSVVQK
                     IEITKDNHDMSLNVKVITDKCIAVTDIETLSSGMRFSIAEVANRDFFNYSNIHSRKFM
                     FIPKYQLCKQSDSTVLYYEDNIDDGNEELYTIRFPARAWSPMDRRNVTHNLTEEKIYI
                     NDGFIEFIELKKEVISDTDYYTMSFALQKELVLYPNANDLFSTLTFCDPNIENVIFID
                     AGHGGHDPGAESRTDDKINEADINLGICLKIEQILLEKGYNVFMTRNEDIYLGFALER
                     GDLANIHNAKLFLSVHVDSYTEESAKGLMTIWRDEKDKEFARIVQKNMVKSAIGADRG
                     LFNQMLAVHARANCPAATVEVGFITNPTESAKLKTDAYQQKLAEGISNGIIEYLELMS
                     NQQP"
     gene            complement(4094..6937)
                     /locus_tag="PLF14_04590"
     CDS             complement(4094..6937)
                     /locus_tag="PLF14_04590"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOT70608.1"
                     /translation="MKNTLKTLLILLIILIPITAYAQQDIYSTCEYTIIDNLKYYKLT
                     SVAGVHKLKVQWSDAEKSVTTSYNGRNIVFNTVNDIVYINGERHYFGDIVLQNGFTYI
                     PEDATTIFNEGYTAYQLNSSSNIKYVNGKITRIDIDANDKLTVTTLKKYDFNQVLYFC
                     VEEFAELYSMTTKVSEGDYIMISNGKTDMYFHVQTDAIMYKGTFFTCGDIFIKDGKHY
                     APDTITAFLLEAFLPDFASHENASEQGVKEDKYTTYIFDNTDIKYVSLKELSDIEGYK
                     YQYYYVNKYAEVKAENISYFVLVPTNIIMKGDNIYNNFKVKEEKDIVYIEYDALAVFG
                     LTDEIKQPIPAEKPSVAEPNVENKPDSEAVAQYIPIRELARKIGGTIEWNDNTFCAIL
                     TVKDVKYVFDIRKMKIYVNDIEISDQEFKFEGGITLVNTSFESYINSTSVLHKALQSE
                     DLLIIKSPDAKIKTHYPLNAPDRVVVDLLNVEKMEGIKQKGLNFNSVRVIPDGNVRRL
                     VVDLSNKAGYQVKQYDGYIEMKINGKVDATQQPVAENKNTEVTDTPKNEMKINVQKDR
                     IGITVADYKGYTVRRISDPDRLEIFIPHTCFNIDKLIPTEGSRYIKAINVRVDKDGTN
                     ISMQLTGQCRYEISEKSKTELIIDIYSQKIINMSYHNAYNRKYIKLKGLSLSDQYGKV
                     NGNVKIANNNLITEISFVDPTFRLTAGVLYVNDDYIEKIQIRRNEETVRMTIYAKQPL
                     QIYVNSTGSSYTNINLLPENNPYSGCVVIDAGHGGFDPGAVAQTVYESKINLSIAKKV
                     EKLLSAEGIKVFMTRDTDDYVGLYERADIANNLNASLFVSIHSNAFTNSSLKGIMTLV
                     YPVSANNSKITGKSLGRTIQRNLVNITGAADRGVIDRPNLVVLNSTKMPAALVECGFM
                     TNPDELKALQSEEYQDILARGIVRGILDTLNGK"
     gene            complement(6969..7271)
                     /locus_tag="PLF14_04595"
     CDS             complement(6969..7271)
                     /locus_tag="PLF14_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70609.1"
                     /translation="MAKKIYPDEYYKKKAVKLVVAHLRRKLEDAEHSDAETFNPWLLE
                     MDALLEKDEFVLAEYVEMRRELNRLIDNIYDVKLRYKVRDSWNSFGRSLDKKAPIK"
     gene            7553..7921
                     /locus_tag="PLF14_04600"
     CDS             7553..7921
                     /locus_tag="PLF14_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70610.1"
                     /translation="MYLFPYEEQYLSGKTENQIVNTNSNLNLLLCNGECNRKYRPLAC
                     RIFPYFPYLDTNGILEVKFDLRAKSICPLQFTDILQIVINKRFIKRIERVFRKLIKHK
                     VFYDYLRNLTDEIVFLEKFH"
     gene            complement(7955..8028)
                     /locus_tag="PLF14_04605"
     tRNA            complement(7955..8028)
                     /locus_tag="PLF14_04605"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7994..7996),aa:Gln,seq:ctg)
     gene            complement(8116..9303)
                     /locus_tag="PLF14_04610"
     CDS             complement(8116..9303)
                     /locus_tag="PLF14_04610"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOT70611.1"
                     /translation="MKQNRINLMRELLSCGTNVPIWHYDGDGHLLDTTAEHLVLDKIF
                     SYIGASDYMLQYGKNHTAPLILGSDMGLQWCAVFERTGNSVYLLGPVFHSEISGSYII
                     ESIDRYHIDPTFQKQYIRLMHEIPVVPNVVFLQYALMMHYCVTGEYLNRSDIQFQPRS
                     GIISPRTGNSEQEDNRYQTRQLLLHMLREGNMNYKQTLADSNHLFGSFIGTNRDSLIH
                     ATIGATGFTALCIHEAIAAGISSDTAYAVGDAYNESMIQCRNIEELTAIAQEMYEDFV
                     FRCHKHRMNPNLSPQIQSCQEYIELYAEKPLYLATLAKRVGYSEYYLSRKFKKETGMS
                     ISNYIKYVRVERSKLMLAGAGVTIAQIADTLHFASSSHFAESFKKITGMTPQQYRTEN
                     QQL"
     gene            9486..10895
                     /locus_tag="PLF14_04615"
     CDS             9486..10895
                     /locus_tag="PLF14_04615"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT70612.1"
                     /translation="MAKNKQSKADRLPIGKFLAWKSSDITSAGVFLIVTTYMTLFCTD
                     HLGMKPLVVGNIILISNIIDFITDLIAAYVIDNTKTKWGKARPYELGAIGMTLCTTLI
                     FITPSGWSETLKIVWVFTMYTFLFGVFNTFRTSANVPYLIRAFDNNRTLIGKVSSYGG
                     IVTTLGSMVISLTFPKLMAELATSDAGWTTLILIYMVPLTILGMFRFIFVKENPEIDA
                     KQLKDKVDFKDIWAMIRKNGYAWLYLLIMFFFQTIQSIGALSYYFKYIVGDIGSAGLL
                     SIMGFVLLPTMFVFPQIMKKLSASQIIALGAVFSCLGYVVNYFAGASMTLLIIGSIIT
                     AFAMLPISYLGNMVEMDLCTYNQYLGLPRMDASIGAIFNGFGSQIGQGFGGWLLGFAL
                     SSAGYVASEGDTIVTQPESAITMIRLLYSLIPMLLMILLAVVAFLLSKLSKKMPEIEA
                     KIAADYKESHHEMNSPSVS"
     gene            10961..13144
                     /locus_tag="PLF14_04620"
     CDS             10961..13144
                     /locus_tag="PLF14_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOT70613.1"
                     /translation="MELKQHFITTNRPFREGAVEVFQQNISTQNVKKATLTITALGLY
                     EVELNGKKVGDALFTPGFTYYPRHLYFQNYDVKGMLNAENILTVYLAQGWYCGRFTFN
                     NKVQIYGKCPAVSWVLTVEDQTGMHRYYSDDASVKAIASPYEYAGFYDGEVYNQSREQ
                     EIFRPVKFTGHVPEIIEESTLHVRIREEMPVQTVTKHGDVTILDYGQNFAGIICINPS
                     KMQDIQLKLRHGEILNPDGSLYTANLRKAKAETVYHKGSGIYRPRFTYMGFRYVELSG
                     VEYEDGLLTAYAIYSDMERTGYFTCDNPLVNQLYSNQIWGQKSNYIEVPTDCPQRDER
                     MGYTGDGHVFAHTGAYNFNTEAFWDKFLKDIRYSQEANKEGYVAPTLPAPPGEQGIGF
                     MSMLGWGNCVCIVPEMLYWHFGTDRYIREYYDCMKRFVECEIRKMGGILGKKDLWIQP
                     SLGDWLAMGRDVKYIAMHNGPVSNAFIVNDLRIMVWAATLLGKTDDAEKYAAQLEKTR
                     AAYIKAFVKKDGTMKDDYQGAYIMALKMVIPAGDLRNKVYTNLTERIRRDGMQTGFFA
                     TEHILPLLADNGDDRLAFDLLLDERCGGWMYQIKAGATTTWERWDALRPDGTVNETKM
                     STDNMVSFNHYSFGSVGKFYYQYILGIQPIEPGFAKIKIKPHIDNRIGAFRGNYKDIH
                     VSYNGEKLHLSTPADTTVILPDGTVHEVSSGNYIFSVKESVNEKI"
     gene            13131..14429
                     /locus_tag="PLF14_04625"
     CDS             13131..14429
                     /locus_tag="PLF14_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HOT70614.1"
                     /translation="MKKFDKDFLLGASTAAHQVEGNNIHSDSWALEHMKYTSFAEPSL
                     DAVDHYNRYEEDIRLLADAGLNCYRFSIEWARIEPAEGQFDKAEVEHYRKVIRCCKNN
                     GVTPVVVMHHFTSPKWLISKGGWEAQSTVEDFKRYCSYLVNELGDELEYVVTINEANM
                     GLQLAAIAKRFIRQMLLPKRKRNSDGNDGQAQVGINLKRMLLNLKYAAAENLELFGTE
                     KVNNFHSMRTPEGDLLIFRAHCAARDAMKAINPHLKVGVSLSLHDIQWKKGGEKQAAK
                     EWDAEFVHYLPYIKNDDFLGVQNYTRNYFGRFGRIDSDRIPGAELTQMGYEFYPEALE
                     NVIRTVAKDFKGDIIVTENGIATSDDTRRVEFIRRAVDGVQNCIADGIPVKGYCYWSL
                     LDNFEWQKGFSKTFGLIAVDRKTQMRYPKESLAYLGSFAK"
     gene            complement(14549..17170)
                     /gene="ppdK"
                     /locus_tag="PLF14_04630"
     CDS             complement(14549..17170)
                     /gene="ppdK"
                     /locus_tag="PLF14_04630"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOT70615.1"
                     /translation="MTKYVYLFNEGNAKMRNLLGGKGANLAEMTTLGLPVPRGFTVTT
                     EACLRYYEDGEQIGKDIEEEILKMLDITEKTVGKKLGDPKNPFLVSVRSGSRASMPGM
                     MDTVLNLGLNDEVVEGLAKLTNNPRFAYDSYRRFVMMFSDVVIGLSKSKFERILDDIK
                     EDKGIVSDTDLTADDMKFVLKKFKEMYKKEMGKEFPQDPKEQLFEAIKAVFRSWNNER
                     AIYYRRMNDIPSNWGTAVNVQEMVYGNMGEDSGTGVAFTRNPSTGEKKLMGEYLMNAQ
                     GEDVVAGVRTPYHIDHLEETNKEVYDQFMDLCMKLENHYRDMQDIEFTIERGKLFILQ
                     TRNGKRTAHAALKIAVDLVKEGLLTKEEALLKVDPKQLDALLHPMFDDKALKAAKVIG
                     TGLAASPGAAAGRVVFCADDAKEMRKKTKDDVILVRLETSPEDIEGMNISQGILTARG
                     GMTSHAAVVARGMGRCCVSGCTELKIDEHKKEFSLSGKTYKTGDWISLDGSTGKIYGE
                     KVATKEPELSGDFATLMSWADEVRTLKVRANADNPADAKQAVSFGAEGIGLCRTEHMF
                     FEADRIRSVRQMILADTKEQREEALNKILPMQRGDFEGIFTAMEGRPVTIRFLDPPLH
                     EFLPKEDREIKELAEVLKVKESVVRAKIDELHEINPMMGHRGCRLSVTYPEIAVMQTT
                     AIIEAAINVSKKGIKIEPELMIPLIFEPKEFAFVKKTVVNTANRIIKDAKVNLKYTVG
                     TMIEIPRAAILADEIAKEAEFFSFGTNDMTQMTYGFSRDDATKFLGAYYDLQILESDP
                     FARLDQKGVGKLVKMAADLGRATRKDIKLGICGEHGADPSTIEFCYLTGLDYVSCSPF
                     RVPIARLAAAQAVINNR"
     gene            complement(17183..18574)
                     /locus_tag="PLF14_04635"
     CDS             complement(17183..18574)
                     /locus_tag="PLF14_04635"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOT70616.1"
                     /translation="MSKEQVTMDKLVSLAKRRGFVYPGSEIYDGLANAWDYGPLGVEL
                     KNNIKAAWWKKFITDNEYNVGLDCAILMNPNVWVASGHVGSFVDPLTDCKNCKTRHRA
                     DELIKEYNKSKSIEMIVDGLDNETLTTYIKEKGIPCPNCGKQDFTEVKQFNCMFKTYQ
                     GVNEDTAAQIYLRPETAQGIFVNFKNVQRTTRRKIPFGIGQIGKSFRNEITPGNFIFR
                     TREFEQMELEFFCEPGTDLEWFEYWRTFCHNWLLSLGLNKDNIRLRDHSEKELSFYSK
                     ATTDFEYLFPFGWGELWGIADRTDYDLKQHSEFSKQDLSYFDPITEEKYIPYCIEPSV
                     GVERLLLAILTDAYDEELLEKDDSRTVLRLHPFLAPVKAAILPLAKRLGDDAFKVYEM
                     IRKKWPAEYDDTQSIGKRYRRQDEIGTPFCFTYDFDSLEDNCVTVRYRDSMKQDRIRI
                     DSIMTFLEENIKL"
     gene            complement(18567..18821)
                     /locus_tag="PLF14_04640"
     CDS             complement(18567..18821)
                     /locus_tag="PLF14_04640"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOT70617.1"
                     /translation="MINKPSVEQLEKKIPVRFVIVNALAKRSREIAGGARVRIETNEK
                     NPITVAAMEIDSGAVRVVDEPIAEELQEEEIITESESVDE"
     gene            complement(18842..19117)
                     /locus_tag="PLF14_04645"
     CDS             complement(18842..19117)
                     /locus_tag="PLF14_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017727251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HOT70618.1"
                     /translation="MEMKFLNIGFGNMVSIERIVAILSPESAPVKRIIQEARDISVLI
                     DATYGRRTRAVIITDSEHVILSPLQPETLERRLSGGVVPDDISEESN"
     gene            complement(19114..19989)
                     /locus_tag="PLF14_04650"
     CDS             complement(19114..19989)
                     /locus_tag="PLF14_04650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOT70619.1"
                     /translation="MAYSMTGYGKSLYENELISMRVEIKSINSKFLDLNLRLPKNISF
                     AEEKVRNLLRSRITRGKIDCYVTVHDMHASFTDIKFDEKVADAYFGFLKSIKRRYNLI
                     AGITPVQIASFPGVINTTESDEDYDKLTDCILEAFNIAIDNILVAKKKEGEILCADCE
                     LKLKEFENLVDNIQKVSADLPAQYKQKLEKRIAELLEDVKTDETRIAQEVAFFADRCC
                     IDEEITRLRSHIKQFSNNLHNETSGKKLDFVLQEMLRETNTIASKANSLDVINNVLEM
                     KCIIEKIREQIQNLE"
     gene            complement(20102..21208)
                     /locus_tag="PLF14_04655"
     CDS             complement(20102..21208)
                     /locus_tag="PLF14_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012060987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOT70620.1"
                     /translation="MMASKVYFTDLHTKHGHNLLTKFENLFKKVGIDELSFENKFTAI
                     KIHFGETGNLAYIRPNYTKVLSDYIRSKKGRPFLTDCNTLYPGSRKNALEHLDTAYQN
                     GFNPFVTGCNVIIADGLKGTDEALIKIDLKHVKEAKIGHAIADADIIISLNHFKGHVS
                     TGMGGAIKNIGMGSGSRAGKMEMHSAGKPQVNRDKCRGCFTCIKSCAHDAISEVNGKA
                     YINHDYCTGCGRCIGSCLFDAIRPSHDETNEILSEKMAEYTYAVLKDKQHFHISFVTD
                     ISPYCDCHSFNDVPIVDDIGIFASFDPVALDKACADAVNEGIVIKGSLLDKNKKMKDN
                     LTTVNPSTDWIHALQYASDIGLGSMQYDLIKLDK"
     gene            21376..22674
                     /locus_tag="PLF14_04660"
     CDS             21376..22674
                     /locus_tag="PLF14_04660"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOT70621.1"
                     /translation="MDFIEKRLLECAVHVPKILIPTNKSDHISWPGFPAVNDDLNASL
                     LAPLNAGNLMNSKDYSSDLKNELNLPERKRYVRTYKNMKRAVRSGFLTEYKGFILTRR
                     INKSGVERYGLLILLDYEDYLSDNPVIAVSREYNRDIANAQADFMTNAIFDLQYAQAL
                     IEDQEATLIEPLVASTFHFKKIFSFNHPVFGIYEGFLIDTPQHFTLISNAFAKIKAKS
                     IKNGKSLFLVHEGVESFESGLIHWNRLKEQYGNRLYRYNPARFHLVELFNIYSPDINL
                     VPYNVLVKDISHDKLVTQFRAKEKIKVETLSSVRELHEAILKRSEKYGSVCYGLICDD
                     VSYLVTFRTSESDIGIINIQDTLSELIQENEHSVSYHINISEMLIEYAEGKHTAIIMP
                     PIKKDTFFDNIAKHGRLNRHAYCHDIPANLRFLFEARFLQ"
     gene            complement(22675..24114)
                     /locus_tag="PLF14_04665"
     CDS             complement(22675..24114)
                     /locus_tag="PLF14_04665"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT70622.1"
                     /translation="MKVVLFALNSSYVHTNLAVRCLKKYAEQQLPNKHEIIIVENNLK
                     DKFDEVLYSLYSLKADVYAFSCYIYNIREILFYAGGIKSLLPDSLIVFGGPEVSYDAE
                     DLLKNHTFIDYVIINEGEEAFADFLEKGSDEKIIDGKIFGKFTQTGIQYSVNETISGN
                     IAYYESSRGCPYKCSYCLSSRISGIRAKTVSHTLQDLLEFEKFSTVSTIKFVDRTFNY
                     NMKRAKDIFQGLLSEKYTKTYHMEMRPELFDEEMFCILEKFPKGKLQMEIGIQSTDNK
                     VLLAIDRPSDIEKAIRNMKKLRRLGNINVHADLIAGLPYDDISSVRESYNSLFYACDR
                     LQLGFLKLLKGTKIREEKDKYAYKFNMNAPYDVYENRFISFDEISSLKRVAALTERYK
                     NSVHFERTIEYITGFSLSPFDFFVGLDRYINRHISSVSQENAFKALYEFSKKEIKSGM
                     TDKEWSDFIDYLKEDYSDCLNRQAPLSYT"
     gene            complement(24111..25262)
                     /gene="dinB"
                     /locus_tag="PLF14_04670"
     CDS             complement(24111..25262)
                     /gene="dinB"
                     /locus_tag="PLF14_04670"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOT70623.1"
                     /translation="MRDILLVDMNAFFIMCESRRDKTLLNKPAAVAGDPKYRSGIILA
                     SNYEARKYGIRVTMTINEALKLYPDLILVKPDHAYYEKCSSQVFKLLYEYTPIIEINS
                     IDEAWMDVTNSRKLFGTPLDIATSIQNRLKDEMGLWCSIGISENKFLAKMASDIKKPL
                     GITTLYKNEIEDVLWPMPVRNLYGIGKVTAEKLINEGVNTIGDLATCDVAYLVKKFGK
                     YGFYLYNRSNGIDDDPVCPVQAENLSCGRMETLAQDIIDIKEAKKVMALLTDSVARTL
                     RRRKLEGSVVTVQIKYNTFKMINRQMQIEQSNLTKDIYNAACKLLEDNWDEDKPVRLL
                     GVSVGSLKGKDMQISMDFQDREKEEKLEETIDELKDKFGKNLIKRGNVL"
     gene            25412..25487
                     /locus_tag="PLF14_04675"
     tRNA            25412..25487
                     /locus_tag="PLF14_04675"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25445..25447,aa:Lys,seq:ctt)
     gene            complement(25512..26471)
                     /locus_tag="PLF14_04680"
     CDS             complement(25512..26471)
                     /locus_tag="PLF14_04680"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOT70624.1"
                     /translation="MGDIKVTDLATGMEIKKQLFVLWNSEHRKASNNKYYMDFMFKDQ
                     TGEINGKKWDADPNEGSQYETGVLYYITGRVTEFQGNLQLNVSYITKAPEEIQKRIAD
                     FVPSAPFPSEDMYKKALTYIESIKDEQLRRLCYMVYKQYEEQLMYYPAAKVLHHSVRG
                     GFLYHITTMLDVAKALSAVYKEINTDILYTGILLHDIMKLREMDSNELGIVTDYSLEG
                     QLMGHIEIGICYIGEMAKKAGLSENKELLIKHMILSHHNIPEHGSTKMPMFLEAELLH
                     HIDLMDARVYDFNNAYETLEKGAISERIVYSLERRVIRPDFDK"
     gene            complement(26444..27145)
                     /locus_tag="PLF14_04685"
     CDS             complement(26444..27145)
                     /locus_tag="PLF14_04685"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT70625.1"
                     /translation="MLRLKNLTKSYDDKVIFQDFSMEIEKNRITCLLGKSGCGKTTLL
                     NVLSSITGEFTVARSDFEKLKYSYIFQEPRLLDNYTVYKNLQFVLDSKHLSKAEMDES
                     IMSCLKMVEMDKYSHYYPNQLSGGMAQRVSIARAFVKDFDVLLMDEPFKGLDPTQKED
                     IMERFLEMWRQNRKTVIYVTHNLEEALCLAYDIHVLGDSPTRIIYTSKNAYKMNDSDR
                     DIERNRLWEILRSQI"
     gene            complement(27111..27875)
                     /locus_tag="PLF14_04690"
     CDS             complement(27111..27875)
                     /locus_tag="PLF14_04690"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT70626.1"
                     /translation="MKKRFLKAAAIILWLLVWQILSVIIGKELLLPSPYITFATLIRL
                     LSQPAFYNQILYSLLRIVAGFLTAVISAFLLGVIAGLNKNVEILLSPFITVIRSTPVM
                     SFIILAIIWFSYNLAPLAVNIIMCFPPIYDNVVKGIRSTDNKLLDMAKVYRVQPVKVF
                     TGIYLASVKPFLMAGMNTAIGLSFRVAVAAEVLANPRYGIGTKLLQAKYNLDTAEVFA
                     WTFVILLLSFLFEQLFKKITEGGRRNAPAEKSDKII"
     gene            complement(27881..28771)
                     /locus_tag="PLF14_04695"
     CDS             complement(27881..28771)
                     /locus_tag="PLF14_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HOT70627.1"
                     /translation="MKKKLGDRYDGYRLRNVDTFFLLIPYIMKTRNDSQIFFEDVADV
                     EELDAFVRKNQENIPGLKLYHVFIAALVRLMATRPRVNRFVCGHKIYARNHIAISMSI
                     KRSMTDDGEETNIKPKFAPESTLKDVVEIVNKEVNLNKESGSENDTDKFAKLVGHMPT
                     FIIKAVINTLMWMDRIGIMPKAAIDFSPFHCSAYLTNMGSLGIKSVYHHLYNFGTTSV
                     FLAMGKREYQYQGSDEGELIKKRIISLKMVVDERICDGFYYASSMRMFSKILQNPEVL
                     LTPPEEVLVDDGIEAPKAKK"
     gene            29029..30390
                     /locus_tag="PLF14_04700"
     CDS             29029..30390
                     /locus_tag="PLF14_04700"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003178935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOT70628.1"
                     /translation="MKVVKFGGSSLSDATQVKKVFNIVTSEPARKFVVVSAPGKRNAS
                     DEKVTDMLIALAEKALANEDVTEQLNKIIQRYRDISTGLGMNDDIIDTITQDLKNRLN
                     SDKSIKGRYLDNLKAAGEDNCAKLVAKYFNLQGEKATYVSPKEAGLLLTAEFGNAQVL
                     PASYQNLKVLHNYEGKVIFPGFFGYSVNGDVVTLPRGGSDVTGAILAVALECDVYENF
                     TDVDYIYTVNPNIVSDPLPIDHMTFKEMRELSYAGFSVFHEEALTPVYYKFTHVNVRN
                     TNNPTAPGTMIHMEVNQNRKHRITGISGDKGFSTITVRKYLMNREVGFVRKILQMLEE
                     ECIPFEHSPSGIDDISIILRSKYLNKEIEDKLICRIKKELGAEEVSIVHNQALVMIVG
                     EKCLDDSETLHKCSEALHEAGIVIKMLNQGSSKLSIIVGIDEADFEKAIRSLYNKFFI
                     EMA"
     gene            30518..31471
                     /locus_tag="PLF14_04705"
     CDS             30518..31471
                     /locus_tag="PLF14_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70629.1"
                     /translation="MKQFLAIYLTLSVIIASVRAIKPKQFFYETSLFSWSTEQTYSEG
                     LTDYLEVLDVCDINSSYQYFSDEMLNSTYPREYIKKLNEQGISTYYLSGNPTWATARR
                     NRSMREKITLVSKYNRKVAEREKFTGIVFDVEPYLLSDWKKDKNNIMDKFLDNMKDSY
                     QYAKEQNLELIVCVPYWFDNDFYHILEQLIADACDKLAVMNYYRNSEIKNIENELELC
                     RKYGKTIICISELQKPVSDSVPDTVTYYNYGLIVMWRMWNHLYSHFDYSKLEFSYHYY
                     KPLRDFLDQIKVPKEPVDIIKPQYQQKIQYPDGISTVPTVY"
     gene            complement(31523..33493)
                     /locus_tag="PLF14_04710"
     CDS             complement(31523..33493)
                     /locus_tag="PLF14_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOT70630.1"
                     /translation="MKKSTITINKVEYDLISVNTVIVGTGAAGFNAADSLYDLGQKDI
                     CIVTEGIKMGTSRNTGSDKQTYYKLTLSGDGDDSVHEMAKTLFDGGSMHGDIALTEAA
                     LSAKGFYKLASIGVPFPHNKYGEYVGYKTDHDPRQRATSAGPLTSKFMTEKLEQQVKS
                     KNIQIFDGFYVIAILQDKGRAYGLVAIDTSKVNRDNYGITVFNCTNIVYATGGPAGIY
                     ETSVYPVSHTGTTGIAFEAGAKGINLTESQYGIASIKFRWNLSGTYQQVIPRYISTDT
                     EGNDEKEFLDEYFTDKGRLFDCIFLKGYQWPFDPRKIENIGSSVIDILVFHETVIKGR
                     RVFMDFRSNPKGFDFSLLNEESYTYLKNSNAFQDTPIKRLEHMNKPAIDLYMNNGINL
                     YKENLEIAVCAQHNNGGLEANIWYESNIRHLFPVGEVNGTFGVYRPGGSALNSTQVSS
                     FRAAQYIAQNYGSAPLSNADFKNLSKDRINELKTLADKLTQNKDKTNVMQQRKRYQRL
                     MTRAGAHIRGLSEVSEAVNTSLKELKAFDTETSANDRAYLADAFRNRDILITQYVYLS
                     AIKEYIERGGKSRGSYLVRDDNGILPLPNLPELFRFKLDDLKSMKKACSVTYDKDTMK
                     VRFDWTDVRPIPVEDNWFENVWADYRNKNIIK"
     gene            complement(33512..34282)
                     /locus_tag="PLF14_04715"
     CDS             complement(33512..34282)
                     /locus_tag="PLF14_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HOT70631.1"
                     /translation="MKKVAIVTGAARGIGNGIAYQLATEGYDIAILDVFAQEDVAKNV
                     DRVIKAGAELLYFKGDLAKTEDRLDFVKEVMNKYGRIDVLVNNAGVGPKVRMDILQTT
                     EESFDFVMNINVRGTYFLTQAVANEMIKCKETLKDCNPKIVNISSMSAYTSSTSRGEY
                     CISKAGVSMITKLFADRLAEYEINVYEIRPGIIYTPMTEVVRAKYDKLIAEGISPIKR
                     WGYPEDIASVVSAFVSGKFPFSTGDVINVDGGLHIQRL"
     gene            complement(34349..35656)
                     /locus_tag="PLF14_04720"
     CDS             complement(34349..35656)
                     /locus_tag="PLF14_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HOT70632.1"
                     /translation="MEYSRFNFKTKKQMEDFCKENEIPLGFSDNLSVLAQSVRIGEKV
                     VKNSLGIHPMEGCDGNADGSCSDLTLRRYKRFANGGAGLIWFEATAVNKNGKANPRQL
                     QITEENLEKYKHVVNMIRKESKGCIIVMQLTHSGRFSKPEGKPAPILECKNDILDRHQ
                     RLQKLDVITDDELEALEEDFVNSAVLAYKAGFDGVDMKACHRYLNSGLLGAFTREGKY
                     GGSFENRIRFMVNCVDKIRAAVNDDNFIISTRINIYDSIPYPYGFGVNKCNYLLPDYD
                     EPVKLAKILYSKGVRLLNVTMGTPYYNPHINRPFDTGEYIPHEHPLEGVARLISGAAE
                     IKKQVPEMVVMGTGYSWLRNFAPYVAAYVKENNMADIIGFGRVSLAYPDFANDILKNN
                     GMVSNKCCICCGKCVELMRAGSTPGCVIKDGEVYLPLYRELNK"
     gene            complement(35758..36351)
                     /locus_tag="PLF14_04725"
     CDS             complement(35758..36351)
                     /locus_tag="PLF14_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70633.1"
                     /translation="MNLQLREMADKCMNVISSLPMVKYCELYGSIANNTEDEFSDIDI
                     KVDVSGYDNGIFMLEVPHLIKDKINVIYYDFAPGLIPNSYIVSIAISIDNPFAIVDIN
                     CIAIPHCTTVLKEHAVNDVFSHTLKVWVANLKHHARGYDCYDDIVRMAKRLGIKDTGS
                     KDEEELLEETLTWLEENQTDKLERYVNSCREKFEELV"
     gene            complement(36426..37001)
                     /locus_tag="PLF14_04730"
     CDS             complement(36426..37001)
                     /locus_tag="PLF14_04730"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HOT70634.1"
                     /translation="MIKRTFTADIETEIKVMITKDEYQILFSKGHNIHTQINHYYKIT
                     DDLTVRIRKMNNEFFLQYKVSNDGVQLKGLKDKTEYSMKLSESDYLIIKNNPEALLTY
                     LKKEKTSDSSVVYKGTLETLRANVTLDVSMPDAEIDMNTYNGFTDYELEWEIDKKQYY
                     KALRILKKYGIDINDRVAGISKYKRLIQSYI"
     gene            complement(37036..40458)
                     /locus_tag="PLF14_04735"
     CDS             complement(37036..40458)
                     /locus_tag="PLF14_04735"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOT70635.1"
                     /translation="MVKWTNQQIKAIKTLNTNILVSAAAGSGKTATLIERIINILSDE
                     KKNVQCENLLVTTFTNAAAGEIRERIRRSLREKVQDNPSDLYIYKQYRNIDRATISTL
                     HSFCSEVIRQNAALINIDPGFSVADKTVADKFLLQAINTVYEQNLSRQDADFNRLLET
                     FGYGKNFNALTDLITSVLKTVKSYPDYHEWLKESAEKTVIKDNIFSSAYGKQIYDHIP
                     EKTDILKRYIDNVQYEIRNTPEGEHYKATLENDLKIIEGIEDTLKYKDWDKLKNCLDE
                     IRFPNLPQKKKDFEEETVNAIKAYRTKLKKAVSSLAALFYDYSDNIIKDCRFMQRPIK
                     KFVETVLQADDVYSKMKVKAKLIDYADMEHMALRCLKSGAEEIYREKYKYILIDEYQD
                     FNYIQEEIINKISRGDNVFAVGDLKQSIYRFRNAQTAIFRDKSQEYEDDPSLGTKLTL
                     SYNFRSRNAILKYVNDIFTDIMTEQLGEVEYSEDEELKYGSKQYENETDESYRPTVIK
                     ICVDNSKMETEDRLSDVEIEAYVIADEIKQLIDSKLMIYDDKLKTTRPVKLSDIVILM
                     RAVSNYGDKYTEILNNCGISAYSEVSIEFLSNKTISDLYALLNVVDNPYQDIYLAQIL
                     KNKVYNFNECEFALIKKDKSDLYDALVSYNEEDRLQSKIQRFLEDFHDIRKFSRNNTV
                     EKTVYYAMTKSGIYYVSREQDLLKRFYVLAVECYKKGIDDLYGFLDFLKSKDKHELSV
                     IYDIMRHNKDNTVNIMSIHKSKGLEFPVVILAGSTRKFNFTDTQGRLIIDRKLGIGSD
                     YINCDNKTYHKHITKNALSLSMEKQSLSEEMRVLYVALTRAKEKLIITGIDKSFDENV
                     IEQEAKCPFDWILPNSYGATFKTLNEKDCENIIRNLTEGEVAETVQARDLNDAAYYQN
                     ILAKTGFEYPYLEETRLNRKVTVTELSKYKSKRYDIDDFYNRYRVQTHKKPMFMQKDS
                     KISAAQKGTIMHKIIASIDLRQLNNPEYISYIKSKITVPEYLNNILAFIGTPLFNDIC
                     EADAIYQEKVFFMPVNTSYISAYTDDKYQSEHEVLLQGVIDCLIIKDGYATIVDYKTD
                     KVEKGNEKEHALRYIKQLDIYSEAVERIMGIKVTRKIIHFFETNTNVELNLL"
     gene            complement(40452..43628)
                     /locus_tag="PLF14_04740"
     CDS             complement(40452..43628)
                     /locus_tag="PLF14_04740"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HOT70636.1"
                     /translation="MSLRILYGPKKKALTKAFIKEINQAIEKDHKYRLIVVVPEQFSS
                     YYEETLASDRGFMKAEVMTFSRLTQRLFDLEYIERNDTVNDTGKTMLLYSLLNKEELS
                     LDYYQKSRKYPGFSVLAMQTIKELRANNISYEDILRISENGDFSYATKTKFAEIAAIY
                     EFYMRSMKEMGLADQTDSYDMLCEHINNTTTFDNSIIWFDRFSFFSAKEFDVIKSLMN
                     KAERINISLCIDYGISGPDSDIFTIPLCTMDKLKEQAKQQRTTFIEERDKTYTEETVQ
                     SQSLDFLFSNLFRSRNEISKGAVKDIEIFKAYDIYEEAEYAAIRIMNAVENRGIRYKD
                     ILCVVSDVSSYERIIKAVFDQYDIPFYINSKKSIADNPLIKFIISILDIYVWDYDIDS
                     TSRFIKNLYSIIDEEDALKLENYIIKWGISAKKAWHNVWDYENNAYDDRMNRLREKIV
                     DSLDAFFNKVRYGCRPEEFARHLYNLIENLRIYEKTDVEENYTESDGAEQAKQCYDSF
                     IDILDQMVIMPDDEKRTVEYYMNILKTAFMETSVGITPVTKDCVILTNVARADIKDYD
                     TMIVLGVNEGLFPGLSDSKSLFNDSDIERIKQYGYEISTDPSQNAVKEEYQIYELMQS
                     PTRKIYLSYHLHDSEGGDKNMSVWLAKVSAIFPNIEVLTRVDIPENEYAHNIKTAFSM
                     LFNGNTSLGDVCKDTRLEKLLYVPSDTANITQSEMSGMLFGDTMNLSATMLERYVACP
                     YSFLMAYGLKFDERPVFEYFSANLGSLKHSILFDAMSLICDDNIDYTYDEIYAICEQS
                     AEKISRTNNIYKRDSVLEYVKKRAVQQAADTITISLDIMKNEKMRPKYLETKFDENGI
                     IKPLVFKTGNVNVRINGKVDRIDTATGENKEYFRVIDYKTSDNDIALYRIKEGLDIQL
                     ALYAYAYHKSVKAQLAGMYYMTIENKYSKVENSQDNSFEDINKVLPGYSFDDRSGSLS
                     ELIDNNTIKKSKVLDSDLQQNIFQSVESTIVTSADNINNGNFAVKPLMDKKVSACKYC
                     PYGMLCGFDSKKDRYRKVEAKKDNEIIW"
     gene            complement(43625..44533)
                     /locus_tag="PLF14_04745"
     CDS             complement(43625..44533)
                     /locus_tag="PLF14_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HOT70637.1"
                     /translation="MISYIDYSEEVKKAKKEGQPVVALESTIISHGMPYPKNVETALM
                     CEDIIRKNNAVPATIAVIDGRLKAGLSHDEIEYLAKAKGILKMSRRDLPAVVAQKQDG
                     AATVAATMIIAALAGIKVFATGGIGGVHREASHTFDISADLQELAKTSVAVVCAGVKS
                     ILDIGLTLEYLETMGVPVLGYQTDKFPAFYTDDSGFGVDYVVNDAEHAADIIRAKWGL
                     GLQGGVVVANPIPKQYSMDKKYIDNIIKEALAEADKRGIKGKKITPFLLDKIKELTAG
                     ESLDANIKLVLNNSNVAAKIAYQLCK"
     gene            complement(44526..45449)
                     /locus_tag="PLF14_04750"
     CDS             complement(44526..45449)
                     /locus_tag="PLF14_04750"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOT70638.1"
                     /translation="MENGYIVIIGGINADITGKAVKDVVRNESNIGKVTLTSGGVARN
                     ICENLVRLGVSCEFITTFSDDHLADMLKTSCAKLGISTKYSYGTKEYSTGTYLSLEDH
                     TGEMVAAVCDLSAIMNMPLSHIDKCNEVIVNATCIVIDCNLSKPMIEYIADKYRDKII
                     IAEAVSSAKVIKLKKCLSDIHTVKLNRQEFSALYGVEYTVDNGKRMSKKYKNRIFVTE
                     GADGSTGYENDQVVHVDAIDVKKIVSVNGAGDSYAAGLAYGIKKGYDTLHCMRLGSIM
                     SFITLSSPGAVSKDITERKILKMLKETGKND"
     gene            45559..45634
                     /locus_tag="PLF14_04755"
     tRNA            45559..45634
                     /locus_tag="PLF14_04755"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45592..45594,aa:Thr,seq:cgt)
     gene            complement(45736..46029)
                     /gene="yhbY"
                     /locus_tag="PLF14_04760"
     CDS             complement(45736..46029)
                     /gene="yhbY"
                     /locus_tag="PLF14_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HOT70639.1"
                     /translation="MITSKQRAYLKGLAQKIQPIIQLGKDGISENTLIMYKEALEARE
                     LVKTTVLKSCEYEPLQVANDIAEAIGAEVVQVIGNKFVLYKQSREKKRINLDL"
     gene            complement(46041..>46553)
                     /gene="cgtA"
                     /locus_tag="PLF14_04765"
     CDS             complement(46041..>46553)
                     /gene="cgtA"
                     /locus_tag="PLF14_04765"
                     /inference="COORDINATES: protein motif:HMM:TIGR03595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HOT70640.1"
                     /translation="FDNINNELAKYNPRLYEHHQICVMNKTDLCTTDEQIKRAEEIEA
                     AIKEKGYEVLKISAATNKGTDILVKRCAELLSKLPDTVIMPEAEEYAVYEHAEDERFT
                     VRKEGNIYYVEGAWATKLVNSINIYNTESLRYFQRQLREKNVIEELEKAGIQEEDLVV
                     IDDFEFEYFR"
BASE COUNT        12804 a        10098 c         8089 g        15562 t
ORIGIN      
        1 tgccactata cttcctgcag cactgcctct gcccggtcct acgggtatac cgtttttctt
       61 tgcgtaatcg atgaaatccg ccgttattaa gaagtagtcg acatatttca tatcgtttat
      121 cactttaagc tcatatttgg ctctttcaac atgcttttcg ttcaatttat ccttgtatct
      181 tcttttcagt ccttcaaata ctatatcctt catgtattcg taatggtcta tgccctcggg
      241 taaaggaaat ttgggaagat gcatttcatt cagattaaag tttacatcgc acatatccgc
      301 tatcttctgt gtattggtta tagcctccgg tacattggaa aattccttca tcatttcatc
      361 ctgagatttg aaatagaact cgtccgacgg gaacctcatc cggttctcat ccaaaacggt
      421 ctttgcagtc tgaatacaaa gcagaatatc atgtgctttt gcatcggttt tttccaggta
      481 atgtacatca tttgtagcaa ctaagggtat atttaactca ttggagagtt ttatcaggta
      541 tttgtttacc agttcctgct ccggtatctt attatattgc aactccagat aaaaattgcc
      601 ttcaccgaat atatccagat actccaaagc tgttgctttt gccttattga aatccttgtc
      661 aataatagcc tcgggtattt ctcctgaaag acaggcgctc aaagcgataa tgccttcgct
      721 gtattgcctt aataactctt tatctacgcg gggcttataa tagtagccgt cctcaaacga
      781 ctgcgaaacc atcttcatta aattatgaag accggtattg tttttagcaa gaagcacaag
      841 atggccttgt gtggaatcta taccgtgtat cttatcggtt ctctttctgc gtgcggtata
      901 tacttcacaa cctattatcg gtttgatacc gacttttctg cactctttat ataactgaag
      961 tatcccgtac atagctccat ggtcggtaat ggcaaccgct ttcataccca aatccttcgc
     1021 tttacttacc atatcgggta ttcttgtcga tccgtcaaga aggctatatt ccgtgtgtac
     1081 atgcagatgt acaaaatcag tcatcgagat actcctttaa ttttcttaat gcattcgctc
     1141 tgtggcttat gccgtttttt tgctcgtcat ccatttcggc gaatgttttg tcataaccgt
     1201 caggcaagaa aaccggatca tatccgaatc ctccgcttcc tttgggttcc gttaatattc
     1261 taccacgaca ttgaccttca aacacgactt ccgttctgtc gctatttatc agtgcaataa
     1321 cacagacaaa tctggcatct ctcttatcac actctttttt ctccatcatt ttaagaagag
     1381 tgcacaaatt caacttgtca atacaagccg aatcggtcat attttcctct gtttttccgc
     1441 ccctgttaac ataaagtcgt ttggaaaaaa caccgggcat attatcaagg cagaaaacct
     1501 ccaaacccga atcatcggca attaccgtat gttcggtaaa tgcctttgca tgtcttgctt
     1561 ttatcaaagc attctttata aaggtgtttt ccgtctcctc gggagtgtcg gaaacgccta
     1621 tatcgctcag agataataat tcaatatcag gcaacagtgc ttttatttct ttaatttttc
     1681 ccttgttgtt actggcaaca acaatagtct tttttttcat cattcagcta aacactttct
     1741 ctgaatttcc attattcttt ctatacattt ctgtgacaag gctattattt catccagttg
     1801 ctctttatca aacgtgcttt tttcaccggt agcctgaatc tccgcaatat tcccgaatga
     1861 atcggttaca acatttacat cgacctgggc attactgtct tccgcatagc ataaatccac
     1921 cattacttcg ttatgcacta tacctgcgct aatcgcagca atctgacata taacgggatt
     1981 cttctcaaca taaccctttt ccttcatcca ctcgaatgca tcgtataaag cgacataacc
     2041 tgccgtgata gatgccgttc ttgttcctcc gtcggcttgt attacatcgc aatcaatgat
     2101 aattgtaaca tcgttaaacg catccagatc acaacatgcc cgtaatgatc ttcctatcag
     2161 cctttctatt tccgaagctc tggaattctt cttaagactt gatatatctc ttttgtttct
     2221 ttgttgcgta gctctgggca gcatattata ctctgcagtc acccagcctt tgccttctct
     2281 gaaaggaggt gattcgaaac tgaccgaagc ggtacaaaga acctttgtct ctccgcattc
     2341 tatgaatgcc gaaccttcgg catacttcat aacatttctt tttatggtga taggtctgag
     2401 ttcatcgttt tgtcttttat caattctcat ggtgcacctg tccttatttt tttactgcaa
     2461 cacaacagga atgttaacag atataatttt aacattttta tcttactgtt acaacaaacc
     2521 gcatgttaaa ttaatgtctg ttacggttgc tgattgctca ttaattcaag atattcgatt
     2581 atgccgttag atataccctc tgcaagtttt tgttgatacg catcggtttt gagttttgca
     2641 ctttcggtcg gattggtaat aaagcctact tccaccgtag cggcgggaca atttgcacgt
     2701 gcatgtaccg ccaacatctg attaaacaag cctctgtccg cacctattgc actttttacc
     2761 atattctttt ggacaatcct tgcaaattct ttatcctttt catctctcca gattgtcatc
     2821 agtccttttg ccgattcttc ggtataagag tccacatgaa ccgaaagaaa cagtttagca
     2881 ttgtgaatat tggcaagatc tcctctttcc aaggcaaatc ccagataaat gtcttcattt
     2941 cgtgtcataa acacgttata tcccttttcc agtaatatct gttcaatctt cagacatata
     3001 cccaagttta tatccgcctc attaatcttg tcatcggttc tggattccgc accgggatca
     3061 tgtcctccgt gtccggcatc tatgaatatg acgttctcta tatttggatc gcagaaagtc
     3121 agagtggaaa acaaatcatt tgcattggga taaagaacca actctttttg taaagcaaaa
     3181 ctcattgtgt aataatccgt atcggaaata acttctttct ttaattcaat aaactcgata
     3241 aaaccgtcat taatatatat tttttcttca gttaagttgt gcgttacgtt tcttctatcc
     3301 atcggtgacc aggcccttgc gggaaatctt atcgtataca actcctcatt accgtcatca
     3361 atattgtctt cataatacaa aaccgtactg tcggactgtt tacacaactg atatttaggt
     3421 ataaacataa acttcctgga atgtatgttg gagtaattga agaaatccct gtttgccact
     3481 tctgcgatgc tgaatcgcat acctgaagat aaagtttcta tatccgtaac ggctatgcac
     3541 ttgtcggtta tgactttaac attcaaagac atatcgtgat tatctttagt aatctcaatc
     3601 ttctgcacaa ccgagttgtc cgggaacagc gactggtcct gataattgta taaattaaac
     3661 tcatccagta aaaccgtgaa gtatatggtg gatttattat taatctcata ccatgatctt
     3721 ttttttgtat cgtccgggtg tcttaattca ttaaagatgt agtctttatt attttcaaaa
     3781 accgtatgga attcacaatc ttttgaaaga ccttcaaaga taagtacttc attctcgtaa
     3841 cttatcttta tctccttcgg ttgccttatt tccgtcggct gcggtgactg cacatccgga
     3901 ccttgtgttt ctgtctgttg aataatagga gttgaagaag ctttaggatt attgttaatt
     3961 aaatcctcat ttgtcagaat aaagataact accgcgataa caatacataa aataaccata
     4021 ataacggcga ttgtgattat cttttttttc atacccgaat ctttataatc gaattccata
     4081 aatcctcact gtactacttc ccgtttagtg tatccagaat acctcttaca atacctcttg
     4141 caagaatatc ctgatattcc tccgactgca aagctttaag ttcatcggga ttggtcatga
     4201 atccgcactc caccaaagcc gcaggcatct tagtagagtt tagcaccact aaattaggtc
     4261 tgtcaataac tcccctgtct gcggcaccgg taatattgac cagattcctt tgaatagttc
     4321 tgcccagcga cttacctgtt atctttgaat tgttagcgga aacgggatat acaagagtca
     4381 ttattccttt cagactgcta tttgtaaatg cattggaatg tatgctcaca aacagtgatg
     4441 cgtttagatt atttgctata tccgctcttt cgtacagtcc gacataatca tcggtatctc
     4501 tggtcatgaa tactttaata ccttcagccg acagcaactt ttccactttt tttgctatgg
     4561 acagatttat cttggactca tataccgtct gtgccacggc accgggatca aatccgccgt
     4621 gaccggcatc aataacaaca catcccgaat aggggttatt ttcaggtaaa aggttgatat
     4681 tggtatagga ggaaccggta gaattcacgt atatttgtaa aggttgcttg gcatatatgg
     4741 tcatccttac agtttcttcg tttcttctta tctgaatctt ctcaatataa tcgtcattta
     4801 cgtatagaac tcctgccgtc agtctgaatg taggatccac aaaggatatt tcggtaatca
     4861 aattattatt tgcaattttt acattaccgt ttacttttcc atattggtca gataaagaaa
     4921 gacctttaag ttttatatat ttcctgttat acgcattgtg ataggacatg tttattattt
     4981 tctgggagta tatatcaata ataagctcgg ttttgctttt ctccgatatc tcatatctgc
     5041 actgaccggt gagctgcata gatatatttg taccgtcctt atcaactctg acatttatcg
     5101 ctttaatata tctggatcct tcggtcggta taagcttgtc gatattaaag caagtgtggg
     5161 gtatgaatat ctccaatctg tctgggtcgg atatccttct taccgtatac cctttataat
     5221 ccgcaactgt aatacctatc ctatccttct gaacattaat tttcatctca ttcttgggtg
     5281 tatctgtaac ttcggtattt ttattctccg ctaccggttg ctgtgtcgca tcaactttac
     5341 cgttaatttt catttcaata tatccgtcat attgttttac ctgataaccg gccttattac
     5401 ttaagtccac aaccagtctt ctgacgtttc cgtccggtat aactcttacg gaattgaaat
     5461 tcagtccttt ttgtttgatt ccctccattt tttcgacatt taataaatct acgactacac
     5521 gatcgggagc attcaaagga taatgagttt taatctttgc gtccggtgat ttgattataa
     5581 ggagatcttc cgactgtaag gccttatgta aaacagatgt tgaatttata taagattcga
     5641 aacttgtatt tacaagtgtt atgcccccct caaacttgaa ctcctgatcg gatatttcga
     5701 tatcatttac atatatcttc atttttctta tatcaaacac atatttgacg tcttttactg
     5761 taagaattgc acagaaagta ttatcattcc attcaattgt tccgcctatc ttccgagcaa
     5821 gctctcttat gggaatatat tgagcaaccg cttctgaatc gggcttattt tcaacattag
     5881 gttcggcaac cgaaggtttt tccgcaggta tcggttgttt tatttcatcc gtcaatccga
     5941 aaacagccag tgcatcatat tcaatatata caatgtcctt ttcttcttta actttaaagt
     6001 tattgtatat attgtcccct ttcattatta tatttgtggg aaccagaacg aagtatgata
     6061 tattctccgc ctttacttcg gcatatttat ttacgtaata gtactggtat ttgtatcctt
     6121 cgatatcgct taattctttc agacttacat atttaatatc agtgttatcg aatatgtatg
     6181 tagtatactt atcctctttc acaccttgtt cggatgcatt ttcgtgactt gcaaaatccg
     6241 gcaggaatgc ttccagcaga aaagcggtta ttgtatccgg agcatagtgt tttccgtctt
     6301 tgataaaaat gtcaccgcag gtaaaaaatg tacctttata catgatagca tcggtctgaa
     6361 catgaaaata catatctgtt tttccgttac ttatcatgat gtagtcgcct tccgatactt
     6421 ttgttgtcat actgtacaat tcggcaaatt cctctacgca gaaataaaga acttgattaa
     6481 aatcatactt ctttaacgtt gtaactgtaa gtttgtcatt tgcatcaata tcaattctgg
     6541 taatcttacc gttaacatac tttatattgc tgctgctgtt taattgataa gccgtatatc
     6601 cttcattgaa tatcgtcgtc gcgtcttctg gtatgtatgt gaagccgttt tgcagcacta
     6661 tatcaccgaa atagtgcctt tcaccgttaa tatatacaat atcgttaacc gtattaaaaa
     6721 cgatatttct gccgttatat gacgttgtaa cgcttttctc ggcatcactc cattgcactt
     6781 taagtttgtg cacacctgca accgatgtca gtttatagta tttgagatta tcaataatgg
     6841 tatactcgca tgttgagtat atatcctgct gcgcatatgc cgtaatcgga attaaaataa
     6901 taagtagaat taagagtgtc tttagtgtgt ttttcatttt ttatcttttg tacccctatt
     6961 tagtttatct atttaatcgg tgctttttta tctaaactcc tcccgaaact gttccatgaa
     7021 tcacgaactt tataacgaag tttaacatcg tatatgttgt caataagcct gttaagctct
     7081 ctcctcattt caacatactc ggctaaaaca aattcatcct tttccagcag tgcatccatt
     7141 tccaagagcc aaggattaaa agtttccgca tcactgtgtt ccgcatcttc cagttttctg
     7201 cgcaaatgtg ctactacgag tttaacagct tttttcttat aatattcatc aggatatatt
     7261 tttttagcca ttttccctta ccatcctctt gttatatctg tctaataaaa aattaacata
     7321 cataaccgga tttaaatttc ttaacttctt ataaattcat gataacataa gcaaatgaaa
     7381 taatcaatat gcaacaatat gctataatct cctcatgaat agccaatttt cgcaaaaaaa
     7441 gatagattgt atatataaaa gatcatataa actcctgaat aacattactc cgttacattt
     7501 tgactgcgga gaattatgta ataacaagtg ttgtaaagat ggatacgaag gcatgtatct
     7561 ttttccctat gaagaacaat atctttcggg taaaaccgaa aatcagatag taaacacaaa
     7621 cagcaatctt aatcttctac tttgcaacgg agaatgtaac agaaaatatc ggcctttagc
     7681 ctgcagaatt ttcccgtact ttccctacct tgacaccaac ggtattttag aagtaaagtt
     7741 cgatttacgt gcaaaaagta tctgtcctct gcaatttacc gatatactac agattgtcat
     7801 caataagagg tttataaaaa gaattgaaag agttttccgg aaattgataa aacacaaagt
     7861 cttttatgac tacttaagaa atcttactga tgagattgtt tttttggaaa aattccattg
     7921 aaaaaaggat gcactccccc tttacatccc cttttggctg ccgaagaagg atttgaaccc
     7981 tcacaaactg atccagagtc aggtgtgcta ccattacaca attcggcaat ataccaagaa
     8041 actataccat aaatgcctta ttacttcaac aactttttta accttccacc taaaaaacaa
     8101 acccctttgc acctgttaca gctgttgatt ttctgtacgg tattgctgcg gtgtcatacc
     8161 ggtaattttc ttgaagcttt ccgcaaaatg actggaagaa gcaaaatgta aggtatccgc
     8221 aatttgagca atggtcacac cggcacctgc caacatcagt ttggaccgct ccaccctaac
     8281 atatttgata tagttactga tggacatacc ggtctctttc ttgaatttcc gggacaggta
     8341 gtattcggaa tagcccactc tcttagctaa agtagcaaga tatagaggtt tttccgcata
     8401 aagttcaata tactcctgac agctttgtat ttggggagag agattaggat tcatacgatg
     8461 tttatgacaa cgaaatacaa aatcttcgta catttcctga gcaatggcag tcagttcctc
     8521 tatattacga cattgaatca tactctcatt ataggcatct cctactgcat aagcagtatc
     8581 cgatgagatt cctgccgcaa tggcttcatg gatgcaaagg gctgtaaaac cggtagcacc
     8641 gatggtagca tgaatcagag aatcccggtt agttccgata aaagagccga acagatgatt
     8701 ggaatcggcc agtgtttgct tataattcat attaccttcc cgaagcatat gcagtagcag
     8761 ttgccgggtt tgatagcgat tgtcctcctg ttcggaattc ccggttctgg gactgattat
     8821 tccgcttctc ggctgaaact gaatatcgct acggttcaga tattctccgg taacacagta
     8881 gtgcatcatt aaagcatact gtaaaaatac tacattcggc actaccggaa tctcatgcat
     8941 caatcttata tattgttttt gaaatgtagg gtcgatgtgg tatcggtcaa tactttcaat
     9001 aatgtaagaa ccggaaatct ccgagtgaaa caccggaccc agcagatata cgctgtttcc
     9061 tgttctttca aacaccgcac accactgcaa gcccatatcg ctgcccagga ttaaaggagc
     9121 ggtatggttt tttccatatt gaagcatata atcggaagca ccgatatagg aaaaaatttt
     9181 gtcaagaacc agatgctctg cagtcgtgtc caacagatgt ccgtctccgt catagtgcca
     9241 gataggcaca tttgttccgc agctgagcaa ttcccgcatc aagttaattc tattctgttt
     9301 cattacatcc atcctgtttt ttgattataa tatcgtaaaa ttgcgataag tacaagatat
     9361 tagcgttttc agacaagtca gcaaaatttt gatatatttg acaaattcat tgtacttcct
     9421 ttgcagtagt tgttctataa tactcttata aattgtattt taaataaaat aaaggagtac
     9481 atactatggc aaagaacaaa caatccaaag cggatcgcct gcctataggc aaatttttgg
     9541 catggaaatc ttccgatatt accagtgccg gcgtttttct tatagttacc acctacatga
     9601 cacttttctg cacagaccat ctgggcatga agccgcttgt agtaggtaat atcattctta
     9661 tcagcaatat cattgatttc ataaccgatt taattgctgc ctatgtgata gataacacca
     9721 agaccaaatg gggtaaagca cgtccttatg aactgggtgc tatcggtatg accctttgca
     9781 ctacattaat ttttatcact cccagcggtt ggagtgaaac ccttaagata gtgtgggtgt
     9841 ttaccatgta cactttcctt ttcggtgttt ttaatacctt ccgaacaagt gcaaacgttc
     9901 cttacctgat tcgtgctttt gataataacc gcacccttat cggtaaggtt tcctcctatg
     9961 gaggcatcgt taccacctta ggttctatgg tgatatctct gacattcccc aagttgatgg
    10021 cagagttggc tacttccgat gccggttgga ccactctgat actgatttat atggtccctc
    10081 tgacaatcct gggcatgttc cgttttatat ttgtcaagga gaatccggaa atcgacgcaa
    10141 agcaactcaa ggataaggta gatttcaaag atatctgggc aatgatacga aagaacggct
    10201 atgcatggtt gtatttgctg attatgttct tcttccagac catacaatct atcggtgccc
    10261 tgtcctacta tttcaaatac atcgtaggtg atataggttc tgcaggtcta ctcagtatca
    10321 tgggtttcgt attgctcccc acaatgtttg tatttcctca gattatgaag aagttgagtg
    10381 catcccagat tattgctctg ggggctgttt tctcctgtct gggttatgtg gtgaattact
    10441 ttgcaggtgc ttccatgacc ttgctgatta tcggttctat catcacggca tttgcaatgc
    10501 tgcctatttc ttatctggga aatatggtcg agatggattt gtgtacatac aatcagtatc
    10561 tgggtctgcc cagaatggat gcttctatcg gcgcaatatt taatggcttc ggatctcaga
    10621 ttggccaagg ttttggcgga tggttgctgg gttttgcact ttcctcagcc ggttatgttg
    10681 cgtccgaagg agatacaatt gtaacacagc ctgaaagtgc cattaccatg attcgcctgt
    10741 tgtacagtct gatcccaatg ttattaatga ttctgttggc tgtggtcgca ttcctgttaa
    10801 gcaagctgag taagaagatg ccggaaattg aagcaaagat tgctgccgat tataaagaaa
    10861 gccatcatga aatgaactct ccgtcagtat cttgatgtga ccggaagaag attaagtaaa
    10921 aactttgatt attacggttt tttagatagg caggtgtaat atggaactta aacaacattt
    10981 tattactacc aaccgtccct tccgggaggg tgcagtggaa gtatttcaac agaatatatc
    11041 tacccaaaat gtaaaaaaag ctaccctcac cattactgct ttgggtcttt atgaagtaga
    11101 gttaaatgga aaaaaagtgg gcgatgcatt atttactccc ggctttacct attatccccg
    11161 ccatctatat ttccaaaact atgatgtaaa aggtatgctt aacgctgaga atatacttac
    11221 cgtgtaccta gctcagggtt ggtactgcgg ccggttcacc ttcaataaca aggtacagat
    11281 ttacggtaaa tgccctgcgg tatcctgggt actgactgtg gaggatcaaa ccggaatgca
    11341 tagatattac tcggatgatg catctgtgaa agcaattgca tccccctatg aatatgccgg
    11401 attctatgac ggcgaagtgt acaaccaatc tcgtgagcag gaaatcttcc ggcctgtgaa
    11461 gttcaccggt catgttccgg aaattattga ggaaagcacc ctgcatgtcc ggatacgaga
    11521 agaaatgccg gtgcaaaccg tcacaaagca tggtgatgta accattctgg attacggtca
    11581 gaattttgca ggcataatat gtattaatcc gtcgaaaatg caggatattc agcttaaact
    11641 gcgtcacggt gagatactga atcccgacgg cagtctctac actgccaatc ttcgtaaagc
    11701 caaagcggaa acagtttacc ataaaggaag cggcatttac cgacccaggt ttacttatat
    11761 gggattccgg tatgtggagt tatcgggtgt tgaatatgag gacggtctgc tgactgcata
    11821 tgccatatat tccgatatgg agcggaccgg ctactttacc tgtgataatc ctcttgtaaa
    11881 ccaactttac agcaaccaga tttggggtca aaagtccaat tatatcgaag tacccaccga
    11941 ctgcccacaa cgcgacgaac gtatgggcta taccggtgac ggtcatgtct ttgctcatac
    12001 cggcgcttat aactttaaca ctgaagcctt ttgggataaa ttcctgaaag atatccgtta
    12061 ttcccaagaa gcaaacaaag aaggttatgt agcccctacc ctgcctgcac ctcccgggga
    12121 gcaaggcatc ggatttatgt ccatgctcgg ttggggaaac tgtgtgtgta tcgttcccga
    12181 gatgctgtat tggcacttcg gaaccgaccg atatattcgg gaatactacg actgtatgaa
    12241 acggtttgtg gaatgtgaaa ttcgcaaaat gggaggtatc ctcggaaaga aggatctttg
    12301 gattcagccc agtctcggtg actggctggc aatgggacga gatgtaaagt acatagctat
    12361 gcacaatggt ccggtgtcca atgcattcat tgtcaacgac ttacgaataa tggtttgggc
    12421 cgcaacactg ctgggcaaga cggatgatgc ggagaaatat gccgcacagt tggaaaagac
    12481 ccgtgctgct tacatcaaag cgtttgtcaa aaaagacggc acgatgaagg atgactatca
    12541 gggcgcatat atcatggcac tgaaaatggt aatacccgca ggtgatttgc ggaacaaggt
    12601 ttatacaaat ctgactgaaa gaatccgtcg ggacggtatg cagacaggtt tctttgcaac
    12661 tgaacatatt ctgcctctgt tagcagataa cggtgatgat agacttgcct tcgaccttct
    12721 gctggacgag cgctgcggtg gctggatgta tcagattaaa gccggtgcaa ccaccacatg
    12781 ggaacgctgg gatgccttgc gacctgacgg caccgtgaac gaaactaaaa tgtcaaccga
    12841 caatatggtg tctttcaatc actattcctt cggttctgtc ggtaagtttt actatcagta
    12901 cattctgggt attcaaccta tagaacccgg atttgcaaag ataaagatta aacctcatat
    12961 tgataaccga atcggagcat tccgtggtaa ctacaaggat attcatgtaa gttacaacgg
    13021 ggagaaactg catctttcaa cacctgcgga tacaacggtg attctgcctg atggtaccgt
    13081 tcatgaagta agttcgggca attatatctt ttctgtaaag gagtcagtta atgaaaaaat
    13141 ttgataagga tttcctgctg ggtgcatcta cagctgctca tcaggtagaa ggaaacaata
    13201 tacacagcga ttcctgggct ttggaacaca tgaaatatac cagttttgcc gaacccagtc
    13261 tggatgcggt cgatcattat aatcgctatg aagaagacat cagacttctt gccgatgcgg
    13321 ggttgaactg ctaccgcttc tccattgagt gggcaagaat tgaacctgca gaaggacagt
    13381 tcgataaagc ggaagtggaa cattatcgca aggttatacg ctgctgcaag aataacggag
    13441 tgacacctgt tgtggtcatg caccacttca cttctccaaa gtggctgata tccaagggcg
    13501 gctgggaagc acaatccact gtggaggatt tcaaacgcta ctgttcctat cttgtgaatg
    13561 aattgggtga tgaactggaa tatgtagtta ccattaacga ggccaatatg ggcttacagt
    13621 tggcagccat tgccaaaagg tttatcaggc agatgcttct gcctaaaagg aaaaggaatt
    13681 ccgacggcaa tgacggccag gcacaagttg gtattaatct gaagcggatg ctccttaatc
    13741 tgaagtatgc cgctgccgaa aatcttgaat tattcgggac ggaaaaagtt aacaactttc
    13801 attccatgcg tacgcccgag ggtgatttgt tgattttccg ggcacactgt gccgccagag
    13861 atgccatgaa ggctatcaac ccccatttga aagtcggtgt ttctctttcc cttcatgaca
    13921 ttcagtggaa aaagggcggc gagaaacaag ctgctaagga atgggatgct gagtttgtcc
    13981 actatctgcc atatatcaag aacgatgatt ttctgggtgt tcagaactac acacgaaatt
    14041 acttcggtcg cttcgggcga atcgattccg acagaatacc gggagccgaa ctgactcaga
    14101 tgggttatga attctatccc gaggcattgg aaaatgtaat acgtaccgtg gctaaagatt
    14161 tcaagggcga tatcatcgtt acggaaaacg gaattgctac aagcgacgat actcgccgcg
    14221 tagaattcat ccgccgtgca gttgacggtg tgcagaactg tattgctgac ggtatcccgg
    14281 taaaaggcta ctgttactgg agcttgctgg ataactttga atggcagaaa ggatttagca
    14341 agaccttcgg tctgattgct gtggaccgca agactcaaat gcgttatccg aaagagagcc
    14401 tggcctatct tggttctttt gcaaaataag tattttaaaa accccggttc cgaagagccg
    14461 gggttccttt aatccttaat ccatattctt caacatcctg aatcgcgact gtgcatttac
    14521 gctatttcgg ttttcaggtt atcacacctt acctgttgtt tattacagcc tgtgccgcag
    14581 caagccgtgc aatgggtact ctgaacggtg aacaggatac atagtccaga cctgtgagat
    14641 agcagaactc tattgttgaa ggatcggcac cgtgctctcc gcatataccc agtttaatat
    14701 ccttccttgt agctctgccc aaatcagctg ccattttaac aagttttcct actccctttt
    14761 ggtccagtct tgcaaacgga tcggattcca gaatctgcaa atcataatat gctcccaaga
    14821 acttggttgc atcatctctg ctgaagccgt aagtcatctg tgtcatgtcg ttggttccga
    14881 aggagaagaa ttccgcttct ttagcaattt catccgctaa aatagctgct ctgggtatct
    14941 ctatcatggt acctaccgta tacttaagat tgacttttgc atctttaatt attctgttag
    15001 cggtgttaac aactgttttc ttaacaaaag cgaattcctt gggttcgaat atgagaggaa
    15061 tcatcagttc cggttcaatc ttaatacctt ttttgcttac gtttatagca gcttcaataa
    15121 ttgctgttgt ctgcataact gcaatttcgg gatatgttac cgaaagacga catcctctgt
    15181 gacccatcat ggggttaatc tcatgcaatt cgtctatctt tgctctgacc accgattctt
    15241 ttactttaag aacttccgcc aattccttga tttctctgtc ttctttgggc aagaactcat
    15301 gtaaaggagg atccaagaac cttatcgtta ccggtctgcc ttccatagcg gtgaatatac
    15361 cttcgaaatc tcctctttgc ataggaagaa tcttattaag agcttcttct ctttgttctt
    15421 ttgtatcggc caaaatcatt tgcctgaccg accttattct gtcagcttca aagaacatgt
    15481 gctctgtacg acaaagtcct attccttcgg ctccgaaaga aaccgcttgt tttgcatctg
    15541 caggattgtc ggcatttgcc cttactttca gagttctgac ttcatccgcc caactcataa
    15601 gtgttgcgaa gtcaccggac aattccggtt cttttgtagc gactttctca ccgtatattt
    15661 taccggtgct gccgtccaat gaaatccagt cgcctgtttt gtatgttttt ccggacagtg
    15721 aaaattcttt cttatgttca tctatcttca attcggtaca tcccgatacg caacatctgc
    15781 ccattcctct tgccacaacg gctgcatgac ttgtcatacc gcctcttgca gtaagaatac
    15841 cctgtgaaat attcattcct tctatgtctt cgggtgaggt ttccagtctg accagtataa
    15901 cgtcatcttt tgtctttttg cgcatttctt ttgcatcatc cgcacagaaa acgactctac
    15961 cggcagcagc acccggagaa gctgcaagac ctgttcctat tacttttgcg gccttcaatg
    16021 ctttatcgtc aaacatggga tgtaaaagtg catccagttg tttgggatct actttcaata
    16081 gtgcctcttc ctttgtaagt aatccttctt ttaccagatc aacagctatt ttcagcgctg
    16141 catgggctgt tctcttaccg tttctggtct gcaatatgaa gagttttcct ctttctatcg
    16201 taaactctat atcctgcata tcacgatagt gattttccag tttcatacaa agatccataa
    16261 actggtcata aacttcctta ttggtttctt ccagatggtc tatatgatac ggtgttctta
    16321 ctcccgcaac aacgtcctct ccctgtgcat tcataagata ctctcccata agcttctttt
    16381 cgcccgtaga gggatttctt gtaaatgcca cacctgttcc cgaatcttca cccatatttc
    16441 cgtataccat ttcctgaaca ttaacggctg ttccccagtt ggaaggaatg tcattcattc
    16501 ttctgtaata tattgctctt tcattattcc acgatctgaa aaccgctttt atagcttcaa
    16561 aaagctgctc cttgggatct tgcgggaact cttttcccat ctcctttttg tacatttcct
    16621 tgaacttctt cagaacaaat ttcatatcgt cagccgtaag atcggtgtcg ctcacaatac
    16681 ctttatcttc ttttatatca tccaatattc tttcgaattt ggacttggaa agacctatta
    16741 cgacatcgga gaacatcatt acgaaccttc tgtaactgtc ataagcaaat cggggattat
    16801 tggttaattt tgccagtcct tcaacaactt catcgttgag tccgagattt aaaacggtat
    16861 ccatcattcc cggcatggaa gcacgggatc ccgacctgac tgaaaccaaa aacggattct
    16921 tgggatcgcc caatttttta cctacggttt tctcggtaat atccagcatt ttaagaattt
    16981 cttcttctat atctttaccg atttgttcgc cgtcttcata atacctgagg catgcttcgg
    17041 ttgtgaccgt aaatcctctc ggcaccggta atcccagtgt cgtcatttcc gcaaggttgg
    17101 ctcctttacc accgagcaga ttcctcatct tcgcatttcc ctcgttaaag agataaacat
    17161 atttcgtcat aaaaacctcc tattatagtt ttatattttc ctcaagaaaa gtcatgattg
    17221 aatcaattct tatcctgtct tgcttcatgc tgtctctgta tcttaccgtt acacaattat
    17281 cttccaaaga atcaaaatca tatgtgaaac agaaaggtgt acctatttca tcctgtcttc
    17341 tgtatcgctt ccctatgctc tgggtatcat catattccgc aggccatttt tttcttatca
    17401 tttcatagac tttgaatgca tcatcaccca gtctcttcgc taaaggaaga atcgccgcct
    17461 taacgggagc aaggaaagga tgaagccgca aaaccgttct ggaatcatct ttttccagca
    17521 attcttcatc atacgcatcg gtcagtattg ccaacaacag tctttcgacg cctactgacg
    17581 gctcaatgca gtaaggaata tatttttctt ccgttatcgg atcgaaatat gataaatcct
    17641 gtttggaaaa ctccgaatgc tgttttaagt cataatcggt tctgtcggca attccccaca
    17701 gttcccccca accgaacggg aacagatatt caaagtcggt agtagcttta ctgtaaaaag
    17761 acaattcttt ttccgaatga tctctcagac gaatattatc cttattcaat cctaaactta
    17821 acagccagtt atggcagaaa gttctccagt attcaaacca ttccaggtcg gttcccggct
    17881 cacagaaaaa ctccagttcc atctgctcga attctctcgt acggaaaatg aagttacccg
    17941 gggttatttc atttctgaaa ctcttaccta tctgtcctat tccgaaaggt atttttcttc
    18001 tggttgttct ctgcacattc ttaaaattaa caaaaatacc ctgtgccgtt tccggtcgca
    18061 ggtagatttg agcggcagta tcttcattaa caccctgata ggtcttaaac atgcaattaa
    18121 actgttttac ttcggtaaaa tcctgttttc cgcaattggg acaaggtatt cccttttctt
    18181 ttatgtatgt cgttaacgtt tcgttatcca atccgtcaac aatcatctct atactttttg
    18241 atttattgta ttctttgatg agttcgtctg ctctgtgtcg tgttttacag tttttacaat
    18301 cggttaacgg gtctacgaaa gatccgacat gaccggaagc tacccagaca ttggggttca
    18361 tgagaatggc acagtccagt ccgacattat attcattatc ggtaatgaat ttcttccacc
    18421 aggcagcttt tatgttattc ttcagttcca ctcccaacgg accgtaatcc catgcgttag
    18481 ccaatccgtc gtaaatctcc gaaccgggat atacaaaacc tcttctctta gccaaagaga
    18541 ccagcttatc catagttacc tgttctttac tcatcaacgc tctcactttc cgtaattatt
    18601 tcttcttcct gtaattcttc cgcaataggt tcatcaacca ctcttacagc accgctgtct
    18661 atttccattg cagcaacggt aataggattt ttctcgtttg tttcaatcct cactcttgcc
    18721 ccacctgcta tttctctgga acgctttgcc aatgcattaa ctattacaaa tcttacaggt
    18781 attttctttt ccaattgttc aactgacggt ttatttatca tttcctatct ccttatgtca
    18841 attaattact ctcttcggaa atatcatccg gaacaactcc tccgcttaat cgcctttcca
    18901 atgtttccgg ttgaagaggc gataaaatca catgttcgct gtccgttatt attacggctc
    18961 tggttcttct gccgtatgtt gcatcaatca gtaccgaaat gtctctggct tcctgtatta
    19021 tcctttttac aggcgccgac tcgggactta gtatcgcaac tattctttct attgaaacca
    19081 tattaccgaa tccgatgttt aaaaatttca tttctattcc agattttgaa tctgctccct
    19141 tattttctct ataatacatt tcatttccaa cacattgttt ataacgtcga ggctgtttgc
    19201 ttttgaagct attgtatttg tttctcttaa catttcctgc agtacaaagt ccagcttttt
    19261 accggacgtt tcattatgca gattattgga aaactgctta atatggcttc tgagacgcgt
    19321 aatctcctca tcgatacagc atctgtcagc aaaaaatgca acttcctgtg ctattctggt
    19381 ttcatcggtt tttacatcct caagaagttc ggcaattctc ttctccagct tttgcttata
    19441 ctgagccgga agatcggcag atactttctg tatattatca accagatttt caaactcttt
    19501 aagttttaac tcacaatcgg cacacagtat ttcgccttct tttttcttgg caacaagaat
    19561 gttgtcaatt gcaatattga aggcttccaa tatgcagtct gtaagtttgt cgtagtcttc
    19621 atcactttcg gttgtattta ttactcccgg aaaagatgca atctgtaccg gagttatacc
    19681 ggcaatgagg ttatatctcc tctttatgga ttttaagaaa ccgaaataag catcggcaac
    19741 cttttcatcg aattttatat ccgtaaaaga tgcatgcatg tcatgtactg taacataaca
    19801 atctatcttc cctcttgtta ttctgcttct taaaaggtta cgtacttttt cttcggcaaa
    19861 agatatattc ttaggcaatc gtaagttcaa atcaagaaat ttactgttta tactcttgat
    19921 ctccactctc atcgatatta attcgttttc atacaggctt ttcccgtatc ctgtcatgct
    19981 gtatgccata cttacgcatc cttatttaat cttaaatatt gtaacataca caacactcac
    20041 atgcaacatt ttacattgca tgtacgtcgt taaaatattg taaaaaacat gcctaaaaag
    20101 cctatttatc cagtttaatt aaatcgtatt gcatactgcc cagcccgata tcgcttgcat
    20161 attgcaaagc atgaatccag tcggttgagg gattaaccgt tgtaaggttg tctttcatct
    20221 ttttgttttt atccaacaga ctgcctttta tgactattcc ttcattaacg gcatcggcac
    20281 atgccttatc caatgcaacc ggatcaaaag aagcgaaaat acctatatca tccactatgg
    20341 gaacatcgtt gaaactgtga cagtcgcagt acggagaaat atccgtcaca aaggatatgt
    20401 ggaagtgttg tttatctttc aataccgcat atgtgtattc cgccattttt tcacttaata
    20461 tttcgtttgt ttcatcatgt gaaggtctga tggcatcaaa caggcaggaa cctatgcatc
    20521 tgccgcaacc ggtacaatag tcatggttga tgtatgcttt tccgttaact tcggaaatag
    20581 catcatgagc acaacttttt atgcaagtaa aacaacctct gcacttatcc ctgttcactt
    20641 ggggtttacc tgccgaatgc atttccatct tacccgctct ggaacccgac cccataccga
    20701 tattcttgat agcaccgccc ataccggtgg aaacatgtcc tttaaaatgg ttaagggaaa
    20761 taataatgtc ggcatcggca atggcatgac ctatctttgc ctcttttaca tgctttaaat
    20821 ctattttaat caatgcttca tcggtacctt taagtccgtc cgctataata acgttgcatc
    20881 ctgttacaaa gggattgaat ccgttttgat aagccgtatc cagatgctcc aaggcatttt
    20941 tcctgcttcc cggatacagt gtattgcaat cggtcagaaa aggccttcct tttttacttc
    21001 tgatatagtc gctcagaacc tttgtataat tgggccttat atacgccaga ttacccgtct
    21061 ctccgaaatg tatctttatc gctgtaaact tgttttcaaa tgataattca tcaattccca
    21121 ccttcttaaa aaggttttca aatttggtca acagattgtg accgtgtttg gtatgcagat
    21181 cggtaaagta tacttttgat gccataattc ttgtcacctc ttacaggata attataacgg
    21241 gttactgcgt gcaattcaaa tcgtacatag tctaaaaagt agaatataaa ggttggtttt
    21301 gctgttttta ttaatattat gctgtggata atccgctgac cgtatggtaa tattattcat
    21361 gaaaaggaac tgaaaatgga tttcatagaa aaacgactgc tggaatgtgc agtgcatgta
    21421 ccgaaaattc ttataccgac caataaaagc gaccatatat cctggccggg ttttcctgcg
    21481 gtcaatgatg atttgaatgc atctttattg gctcctctta atgcggggaa cttaatgaat
    21541 tccaaagact attcttcgga tttgaaaaat gaactcaatc ttcccgaaag aaagcgatat
    21601 gtacgcactt acaagaacat gaaaagggct gtacgttcgg gttttttaac cgagtataaa
    21661 ggatttattc tgacaagacg aataaataag tcgggtgtcg agagatacgg attgcttatt
    21721 ctgctggatt atgaagatta tctttccgac aatcccgtaa tcgcggtatc aagggagtat
    21781 aacagggata tagctaatgc acaggccgat tttatgacaa acgcaatctt cgatttacag
    21841 tatgcacagg cccttattga agatcaggag gcaaccctta tagaaccttt agtggccagc
    21901 acttttcatt ttaagaagat attttctttt aatcatcccg ttttcgggat atatgaagga
    21961 tttctgattg atacaccgca gcatttcacg cttataagca atgcttttgc aaaaatcaag
    22021 gctaaaagta tcaaaaatgg caaatcactt ttcttggttc atgaaggagt ggaatctttt
    22081 gaaagcggtc ttattcattg gaacagattg aaagaacaat acggaaaccg actttacagg
    22141 tataaccctg cacgtttcca tcttgtggaa ttatttaaca tatacagtcc cgatatcaac
    22201 cttgttccgt acaatgtact ggtaaaagac atttctcacg acaagcttgt tactcaattc
    22261 agagctaagg aaaagatcaa agtggaaaca ttatcttcgg taagagaact gcatgaagcc
    22321 atattgaagc gatcggaaaa atacggctcg gtttgttacg gacttatctg cgatgacgtg
    22381 tcctatctgg ttactttcag aacttccgaa tcggatatag gtattataaa tatccaggat
    22441 actctgtccg aactcataca ggagaatgaa cattcggtct cctaccatat caatatttcg
    22501 gaaatgctca ttgaatatgc cgaaggaaag catacggcta ttataatgcc gcctattaaa
    22561 aaagatacat ttttcgataa tattgccaag cacggacggt taaaccgaca tgcatattgt
    22621 catgacatac ccgctaactt gagattttta tttgaagcaa gattcttaca atagttaagt
    22681 ataactcaaa ggtgcctgtc tgtttaaaca gtcactgtaa tcttctttca ggtagtctat
    22741 gaaatccgac cactctttat cggtcatacc gcttttaatc tctttttttg aaaactcata
    22801 cagagctttg aacgcatttt cctgagaaac ggaagatata tggcgattta tatatctatc
    22861 caatccgaca aagaaatcaa acggacttaa agaaaatccg gttatatact caattgttct
    22921 ttcgaaatga acactgtttt tgtatctttc ggtcaatgcg gctactcttt tcagactact
    22981 tatttcatca aaagaaataa agcgattttc ataaacatca tatggggcat tcatattaaa
    23041 cttatatgca tatttgtcct tttcttcacg tatttttgtt cccttaagta acttcaaaaa
    23101 tcccagttgc aaccggtcac aggcataaaa caatgagtta tatgactctc ttaccgaaga
    23161 tatatcgtca taaggtaatc ccgcaatcag gtccgcatga acattgatat tacctaatct
    23221 tctgagtttt ttcatatttc ttattgcctt ttcaatatcc gaaggtctgt caatggccaa
    23281 aagaacttta ttgtcggtac tctgaatacc aatctccatc tgcagcttac ctttagggaa
    23341 tttttccagg atacaaaaca tttcttcatc aaataactcc ggacgcattt ccatatgata
    23401 ggtttttgta tatttctcac ttaacaggcc ctgaaaaata tcttttgctc ttttcatatt
    23461 ataattaaag gtcctgtcca cgaatttaat agtggagaca gtactgaact tctcaaattc
    23521 cagaagatcc tgcaatgtat gagaaaccgt tttagctctt atgcctgaaa tacgggaaga
    23581 taagcaatat gagcacttgt aaggacaacc tcttgaagac tcataatatg caatattacc
    23641 cgaaattgtc tcatttacgg agtattgtat acctgtttgt gtaaatttac cgaatatctt
    23701 accgtctatt attttttcat cactaccctt ttccagaaaa tcggcaaatg cctcttcccc
    23761 ttcatttatt atgacataat caataaatgt atgatttttc agtaaatcct ccgcgtcata
    23821 agatacttcc ggtcctccga atactatcaa tgaatccgga agaagacttt ttataccgcc
    23881 ggcatagaag agaatctcac ggatattgta aatatagcag gaaaaggcat acacatctgc
    23941 cttcagcgaa tagagcgaat atagtacctc gtcaaactta tccttaagat tgttttccac
    24001 aataattatt tcatgcttat tcggaagctg ctgctcggca tattttttta agcatcttac
    24061 tgctaaattg gtatgaacat aggagctgtt gagtgcaaac aatacaactt tcataaaaca
    24121 tttcctcgtt ttataagatt cttaccgaat ttatctttaa gttcgtcgat agtttcttcc
    24181 aacttctctt ccttctctct gtcctgaaaa tccatgctta tctgcatatc tttccccttc
    24241 aatgaaccga ccgatacgcc taaaagtcgc acaggcttat cctcatccca gttatcttcc
    24301 agcaatttac atgccgcatt atatatatct ttcgtaagat tgctctgttc aatctgcatc
    24361 tgacggttaa tcatcttaaa agtattgtat ttaatctgaa cggtgacaac gctcccttcc
    24421 agtttccttc ttctcaatgt tcgtgctacc gaatcggtaa gcagtgccat aactttcttt
    24481 gcttctttta tatcgataat atcctgtgct aaagtttcca tacgtccgca ggaaagattt
    24541 tccgcctgca cgggacacac cgggtcgtca tctatgccgt tggacctgtt atacagataa
    24601 aagccgtatt taccgaattt cttaacaaga taagcaacat cacatgtagc caaatcccct
    24661 attgtgttta ctccttcgtt aattaacttt tccgctgtga ctttacctat tccgtacaga
    24721 tttctgacag gcatgggcca taaaacgtcc tctatttcat ttttatacag tgtggttata
    24781 cccaaaggct tctttatgtc cgatgccatt tttgcaagaa acttgttttc gctgatacct
    24841 atcgaacacc ataaacccat ttcatctttc agcctgttct gtatcgaggt tgcaatatca
    24901 agaggggttc cgaagagttt tctgctgttt gtgacatcca tccatgcttc atctatgctg
    24961 tttatctcta ttatcggtgt gtactcatac agcagtttaa atacttgcga agaacatttt
    25021 tcgtaatatg catgatcggg ttttaccagt atcaggtcgg gatacaactt aagagcctca
    25081 tttatggtca tggtcactct tattccgtat tttctggctt catagttgga ggccaatata
    25141 atgccggaac ggtatttggg atcacctgca acagcggcgg gtttattcag aagagtttta
    25201 tctcttctgg actcacacat tatgaaaaag gcattcatat caaccagcaa tatatcccgc
    25261 attgaaaact ccgacggtat tgttataaag atatgatata tcaatgaatt ttacttgtca
    25321 aaaggagcaa aacggatatc ggcattactt aaataactga aaaatagggt tgacacgaca
    25381 ctgatatgaa attaaaatac tagttgcgcc tgtgccatta gctcagatgg tagagcacct
    25441 gactcttaat cagggtgtcc ggggttcgat cccccgatgg cgcaccaaaa gaaggattta
    25501 tgccttcttt tttatttatc aaaatccggt ctgataactc ttctttccag tgaataaaca
    25561 attctttcgc ttattgcacc tttttccagt gtttcatatg cattattgaa atcataaact
    25621 cttgcatcca tcagatcgat atgatgaagc aattccgctt ccaggaacat gggcattttt
    25681 gtacttccgt gttccggtat gttgtgatgg cttaatatca tatgttttat caacagctct
    25741 ttattctccg ataagccggc tttcttggcc atttcaccga tatagcaaat acctatctca
    25801 atatgcccca tcaactgccc ttcaagggaa taatctgtga caatacctaa ttcatttgaa
    25861 tccatctccc gcaatttcat tatgtcatgc agtaaaatgc cggtatagag aatatccgta
    25921 ttaatctctt tatacacagc tgacaatgct tttgctacat ccagcatggt tgtaatatga
    25981 tagagaaatc ctcctcttac cgaatggtgt agcaccttag ctgcgggata atacattaat
    26041 tgttcttcat attgcttgta gaccatatag cataatcttc tcagttgctc atcctttatg
    26101 ctttcgatat atgtcagtgc ttttttgtac atatcttccg atggaaacgg tgcactcggt
    26161 acaaaatccg caattctttt ctgtatttct tccggcgctt tggttatata tgatacattc
    26221 agttgtaaat taccctgaaa ctccgtaact ctgccggtaa tataatacaa tacaccggtt
    26281 tcatattgcg acccttcatt gggatcggca tcccatttct taccgtttat ctcccctgtt
    26341 tgatctttga acataaagtc catatagtac ttattattgg atgctttcct gtgctcgctg
    26401 ttccataata cgaacaattg cttctttatt tccattccgg ttgctaaatc tgtgacctta
    26461 atatctccca taacctgttc ctttcaatat ccctgtcgga atcattcatt ttatatgcat
    26521 tcttgcttgt atatattatt cttgtcggac tgtctcccaa cacatgtata tcataagcca
    26581 aacacagagc ttcctccaga ttatgtgtta catatatgac ggtttttctg ttttgtctcc
    26641 acatttccag gaatctttcc attatatctt ctttttgagt gggatccagt cccttaaaag
    26701 gttcatccat aagaagcaca tcgaaatctt ttacaaaggc tcttgcaatg gagactctct
    26761 gagccatacc gccgcttaac tgatttggat aataatggga atatttatcc atttccacca
    26821 ttttaagaca ggacattata ctctcatcca tttcggcttt ggacagatgt ttgctgtcca
    26881 atacaaactg caggttttta tacacggtgt aattatccag caatctgggc tcctgaaata
    26941 tgtatgaata ctttaatttt tcaaaatcgg aacgcgccac cgtaaattct ccggttatgg
    27001 aggataagac attcagtaat gtggtttttc cgcagccgga tttacctaaa agacaggtaa
    27061 tcctgttctt ttcaatttcc atactgaaat cttggaaaat tactttatca tcatatgatt
    27121 ttgtcagatt tttcagccgg agcattgcgt cttccgcctt ccgtaatttt cttaaataac
    27181 tgctcaaata aaaagctcag taacagtata acaaatgtcc aggcaaagac ttccgcggta
    27241 tccagattat actttgcctg taaaagtttt gtccctatac cgtatcgggg attagccaac
    27301 acttcggcgg caactgcaac tctgaaagaa agacctatag cggtattcat tcccgccata
    27361 agaaaaggtt ttaccgatgc cagatatatc ccggtaaata cttttacagg ttgtacccga
    27421 taaacttttg ccatatccag aagtttgttg tcggtactcc taatgccttt tacaacattg
    27481 tcatatatcg gcggaaagca cattatgata ttaaccgcca gcggtgccag attgtaactg
    27541 aaccatatta ttgcaagtat tatgaaactc ataaccggtg tggaccgtat tacggtgata
    27601 aagggagaaa gcaatatctc cacatttttg tttaaaccgg ctattactcc taataaaaat
    27661 gccgatataa cggcagttaa aaaccctgcc actattctta acagcgagta cagaatttgg
    27721 ttatagaaag ccggttggga taataacctt ataagtgtgg caaaggtaat gtacggcgaa
    27781 ggcaaaagaa gctctttacc tattatcaca gataaaatct gccataccaa gagccacaat
    27841 attattgctg ccgcttttaa aaaccttttt ttcattaacc tcactttttt gctttaggtg
    27901 cttctattcc gtcatcaact aacacttctt ccggaggtgt cagtaaaact tctggattct
    27961 gtagtatctt ggaaaacatc ctcatagatg atgcatagta aaatccgtca catattcttt
    28021 catccacaac cattttcaga cttattattc tctttttaat caactcgccc tcgtccgagc
    28081 cctgatactg atactccctt tttcccattg cgagaaatac cgatgtcgtt ccgaagttat
    28141 ataaatggtg ataaaccgat ttaataccca aagaacccat atttgttaaa tatgcactgc
    28201 aatggaacgg tgaaaaatca atggccgctt taggcattat acctattctg tccatccaca
    28261 tcaaagtatt gattaccgct ttaattataa atgtcggcat atgccctacc agtttagcaa
    28321 acttatcggt atcattttcc gaaccgcttt ccttattaag gttaacttcc ttattaacaa
    28381 tttccactac atccttaagt gttgattccg gagcaaattt cggtttgata ttggtctctt
    28441 ccccgtcatc cgtcatgctt ctttttattg acatggatat tgctatatga tttcttgcat
    28501 atattttatg tccgcacaca aatctgttga ctctcggtct tgttgccatg agccttacaa
    28561 gagcggctat aaatacatga taaagcttaa gtccgggaat attctcctgg ttcttcctga
    28621 caaaagcatc caattcctcc acgtccgcca catcttcaaa aaatatctga ctgtcatttc
    28681 tggttttcat aatatacggt ataagaagga aaaacgtatc cacatttctc aatctgtacc
    28741 cgtcatacct gtcgcctaat ttttttttca tatgtttccc cccattgtcc atgctattgt
    28801 atcataatcg gtaagatata aaaataggca ttatttatac ttaattatat ttaaacaata
    28861 tatccttaaa ttttacagag tattaaacat ttgaggggtg aaataatcat aggttattga
    28921 cactgctttc aatgcttctt gacaattaac atgtttgtaa ataaaatcta atgaatgatt
    28981 tgacctctat atttttggtc aggtcacgga taaaaggaga cagatatgtt gaaagtagtt
    29041 aaattcggcg gttccagcct ttcagatgca acacaggtaa aaaaagtgtt taatatcgtt
    29101 acttccgaac ctgcaagaaa gtttgtggtt gtttccgctc cgggcaaaag aaatgcttcc
    29161 gatgaaaaag taaccgatat gcttatagca ttagcggaaa aggcccttgc aaatgaggat
    29221 gtaactgaac aacttaataa aataattcaa agatacagag acatttccac aggtctcggc
    29281 atgaatgacg atattataga tacaattaca caagacctaa agaacagact taattcagat
    29341 aaatcgataa aaggaagata tctggataac cttaaagccg caggagaaga taattgcgca
    29401 aagcttgtag cgaaatattt taatctgcag ggtgaaaaag caacttatgt atctcccaaa
    29461 gaagcaggtc tgctgctgac tgccgagttc ggtaacgccc aggtgcttcc ggcatcctat
    29521 cagaatctga aggttttaca taattacgag ggtaaagtta ttttcccggg atttttcggc
    29581 tattccgtta acggcgatgt ggtcacgcta cccagaggcg gttccgatgt taccggtgct
    29641 atattggcgg ttgctctgga gtgtgatgtt tatgaaaact tcaccgatgt tgattacatt
    29701 tacacggtaa accccaatat tgtatccgac cctctcccga ttgaccatat gactttcaag
    29761 gagatgcgcg aactttcata tgccggtttc agtgtgtttc atgaagaggc tctgactcct
    29821 gtttattaca aattcactca cgttaacgtc aggaatacca ataatcctac cgctcccggt
    29881 accatgatac atatggaagt taaccaaaac agaaaacatc gtattacggg tataagcggt
    29941 gataaaggat tttccaccat taccgtaaga aaatatctta tgaaccgtga agtaggtttt
    30001 gtgcgtaaaa tattacagat gctggaggaa gaatgcattc cgtttgaaca ctccccatcg
    30061 ggaatagatg atatttccat aatacttcgt tcaaaatact taaacaaaga aattgaggat
    30121 aaacttatct gtcgtattaa aaaggaactg ggtgcggagg aagtttctat agttcataat
    30181 caggctttgg taatgatagt aggcgaaaaa tgtcttgatg actccgaaac acttcataaa
    30241 tgttccgaag ctttacatga agccgggata gttataaaga tgctcaatca gggatcttca
    30301 aaattaagta ttattgtagg aattgatgaa gcggattttg aaaaagcaat aaggagtctt
    30361 tacaataagt tctttattga aatggcatag tatattataa aggtaactca ctttgaacag
    30421 ttgtgaccac ggtaatacta ccctttgttc gctgtttcct tatattgttt ttcagtatat
    30481 aatttattaa tacagcatag tgttttatga ggaatatatg aaacagtttt tagcaatata
    30541 ccttacatta tccgttatta ttgcatcggt acgagcaata aaacctaagc agttctttta
    30601 tgaaacatca ctcttttcct ggagtaccga acagacatat tccgaagggt taaccgatta
    30661 tcttgaagtt cttgatgtat gtgatattaa ttcttcatat caatatttct ccgatgagat
    30721 gctcaacagt acatatccga gagaatatat aaaaaagctt aatgaacaag gtatctccac
    30781 ttattatctt tccggtaatc ccacatgggc aaccgcaaga agaaacagaa gcatgaggga
    30841 aaagattact cttgtatcta aatacaatag aaaagttgcg gaaagagaaa aattcacagg
    30901 catagtgttt gatgtggaac cgtatctttt atccgattgg aagaaagaca aaaataatat
    30961 aatggataaa tttcttgata acatgaaaga ttcctatcaa tatgctaaag agcaaaatct
    31021 ggaattgata gtgtgtgttc cctattggtt tgacaatgat ttttatcaca ttcttgaaca
    31081 actgattgcc gatgcatgtg ataaactggc tgtaatgaac tattacagga acagtgagat
    31141 taagaatatt gaaaacgaac tggaactatg caggaaatac ggtaagacaa tcatatgcat
    31201 atccgaactg caaaaacccg tttccgattc cgtaccggat accgtcacat actataatta
    31261 cggacttatt gtcatgtgga gaatgtggaa tcacctatac tctcactttg attattccaa
    31321 actggagttt tcctaccatt attacaaacc tctgagagac ttcctggatc aaatcaaggt
    31381 tccgaaagaa cccgttgaca taataaaacc tcaatatcaa caaaaaatac agtacccgga
    31441 cggtatttct actgtaccga ccgtgtactg atgtgatttt ctgatttaat taataattac
    31501 cttttacaaa aacaatcttt tcttatttta ttatattctt attccgataa tccgcccaga
    31561 cattctcaaa ccagttgtct tccacgggta taggtctgac atcggtccaa tcaaacctta
    31621 ctttcatagt gtccttatca tatgttacgg aacaggcttt tttcatggat tttaaatcat
    31681 ccagtttgaa tcggaacagt tccggaagat tcggtaacgg aagaattccg ttatcgtctc
    31741 ttacaaggta ggaacctctg gattttccgc ctctttctat gtattccttt atagcactta
    31801 agtatacata ctgagttata agaatatcac ggtttctgaa ggcatcggcc aggtatgctc
    31861 tgtcgttagc ggatgtctcg gtatcaaatg cttttaactc ttttaagctt gtatttacag
    31921 cttccgatac ctcactcaat cccctgatat gtgcacccgc tctggtcatc agtctctgat
    31981 agcgttttct ttgctgcata acattggttt tatccttgtt ctgtgtcaat ttatcggcaa
    32041 gagtctttaa ttcatttatt ctgtcctttg ataaattctt aaaatcggca tttgacaacg
    32101 gtgcggaccc gtagttctgt gcaatatatt gtgctgctct gaatgaggaa acctgtgtgg
    32161 agttaagagc gcttccgccc ggtctgtata caccgaatgt gccgtttact tctcctaccg
    32221 ggaacaaatg acggatgttg ctttcatacc agatatttgc ttccaagccc ccgttattat
    32281 gctgtgcaca taccgctatc tccagattct ctttgtataa gttgataccg ttattcatat
    32341 ataagtcaat tgcaggctta ttcatatgct ccagtctttt tataggtgta tcctgaaatg
    32401 catttgagtt ctttaaatag gtataggatt cttcgttaag taacgagaaa tcaaaacctt
    32461 tgggattgct cctgaagtcc atgaaaactc ttcttccctt tatcaccgtt tcatggaaca
    32521 caagaatatc aataaccgaa gagcctatat tctcaatctt tctcggatca aacggccatt
    32581 gatagccttt taagaaaatg cagtcgaaaa gtcttccttt atcggtaaaa tactcatcca
    32641 gaaactcttt ttcgtcatta ccttccgtat cggtggatat atatctgggg attacctgct
    32701 gatatgtacc cgacagattc catcggaatt taatggatgc gattccgtat tgtgattcgg
    32761 taagattaat accttttgca cctgcttcaa aggcaattcc cgtggttccc gtatgactta
    32821 caggatatac cgatgtctca tatattcctg cgggtccgcc ggtagcataa acgatatttg
    32881 tacaattaaa tacggtaata ccgtaattat cccgatttac cttacttgta tcaatggcaa
    32941 ccaaaccgta tgccctgcct ttatcctgaa gtattgcgat aacataaaag ccgtcgaata
    33001 tttgaatgtt tttgcttttg acctgttgct ccagtttctc cgtcataaac tttgaggtaa
    33061 gcggtcctgc cgacgtggct cgctgacgag gatcatggtc ggttttatac ccgacatact
    33121 ctccgtactt attgtgagga aacggtacgc ctatggaagc cagtttatag aagcctttag
    33181 ccgataatgc cgcttcggtc aacgctatgt ctccgtgcat actgccgccg tcaaacagtg
    33241 ttttagccat ttcatgaaca gaatcatctc cgtctccgga taatgtaagt ttatagtatg
    33301 tttgcttatc cgatccggtg tttctggaag tacccatttt tatgccttcg gttacgatac
    33361 atatatcctt ttggcctaaa tcatataaac tgtccgcagc attaaatccc gcagcaccgg
    33421 ttcctactat tacagtgttt accgagataa gatcatattc aaccttgttt atagttattg
    33481 tacttttttt catcattccc tcctgaagtt tctatagcct ctgtatatgc aagcctccgt
    33541 ccacatttat gacatccccc gtcgagaacg ggaatttacc cgatacgaat gccgaaacga
    33601 cagatgcgat gtcttcgggg tatccccatc tcttaatggg agaaatacct tccgctatca
    33661 gtttgtcgta tttggctctt accacctcgg tcataggtgt ataaatgatt cccggcctta
    33721 tctcgtatac attaatctca tattccgcca gtctgtctgc aaaaagtttg gttatcatgc
    33781 tgacaccggc tttggatata caatactctc ctctggatgt cgaactggta tatgccgaca
    33841 tggagcttat atttactatt ttagggttgc aatctttcaa agtctcttta cacttaatca
    33901 tttcatttgc caccgcctga gtaagaaaat atgtgcctct gacattaata ttcattacga
    33961 aatcaaagct ctcttcggtt gtttgcaaga tatccatacg tactttggga cctactcccg
    34021 cattatttac cagaacatca atcctgccgt atttattcat tacctctttt acgaaatcca
    34081 gtctgtcttc cgtctttgca agatcgcctt tgaagtataa gagttcggca cccgctttta
    34141 taactctgtc aacattctta gcgacatcct cctgtgcaaa tacatccaaa atggctatat
    34201 catatccttc ggtagccaac tgatacgcta taccgtttcc tattcctctt gccgctcctg
    34261 ttactattgc aacttttttc atctttccat tacctccaat cactttgctt tatatcactt
    34321 caattctgaa actaatattt ccattaagtt atttattcaa ctccctgtat aacggcagat
    34381 acacttcccc gtctttaata acacatccgg gagtgctgcc cgctctcata agctccacac
    34441 attttccgca gcagatacag catttattgg aaaccatacc attgtttttt aatatatcat
    34501 ttgcaaaatc cggataagcc aaagaaactc tgccgaatcc tataatatcc gccatattat
    34561 tctctttaac ataagcggca acatacggag cgaagttcct taaccaggaa tagcccgttc
    34621 ccatgacaac catttccggt acctgctttt taatttccgc ggcacccgaa atgagccttg
    34681 ccactccttc cagaggatgt tcgtgcggta tatattcgcc cgtatcgaaa ggtcggttga
    34741 tgtgggggtt atagtaagga gtacccattg ttacattcag caatcttact ccttttgaat
    34801 agagaatctt cgccagtttt acaggctcat cgtaatcagg cagaagataa ttgcatttat
    34861 tcacgccgaa tccgtaagga tacggaatgg aatcgtagat atttatgcgt gtggaaataa
    34921 tgaaattgtc gtcatttacg gccgctctta tcttatctac acaattaacc ataaatctga
    34981 tacggttttc aaagcttccg ccgtactttc cttctcttgt aaatgcccct aaaagaccgg
    35041 aattgagata tctgtgacat gctttcatat ccacaccgtc aaagcccgct ttatacgcca
    35101 atacggcgct atttacgaaa tcctcttcca aagcttccag ttcatcatcg gttataacgt
    35161 ccagcttttg caatctttga tgtctgtcca atatatcatt tttgcattcc agtatgggag
    35221 caggcttgcc ttcaggtttg gaaaatctgc ccgaatgggt taattgcata acaattatgc
    35281 aacctttcga ttcttttctg atcatgttta caacatgctt gtatttttcc agattttcct
    35341 ctgttatctg caactgcctc ggattggctt taccgttctt attgacagcg gttgcttcga
    35401 accatataag gccggcacct ccgttagcaa acctcttgta tcttcttaag gtcagatcgg
    35461 aacaggaacc gtcagcatta ccgtcacaac cttccatagg atgtatgcct aaactgttct
    35521 ttacaacttt ttcacctatt ctgaccgatt gagccaaaac acttaaattg tcggaaaaac
    35581 ccaacggtat ttcattctct ttgcagaaat cttccatctg tttttttgtt ttgaaattaa
    35641 atcttgaata ttccatacta tctcctttta caagcatata ccactccatt aattgtactt
    35701 gataagggat attaaaacaa cataacctac cttgagtttc ctgctttttt cacatcctta
    35761 tactaattcc tcaaattttt cgcgacacga gtttacatat ctttctaatt tatctgtttg
    35821 attttcttca agccaagtca atgtttcttc taataattct tcttcgtcct ttgaaccggt
    35881 atcttttatt cctaatcgct tggccatacg aacgatatca tcatagcaat cgtaccctcg
    35941 cgcatgatgt tttaaattag ccacccaaac cttcaatgtg tgcgaaaata catcgtttac
    36001 tgcatgttct tttaaaacag ttgtacaatg cggaatagct atacagttaa tatcaactat
    36061 tgcaaatgga ttatcaatgc ttatcgcaat tgaaactata taactattcg gtataagacc
    36121 aggtgcaaag tcataataaa taacattaat tttatctttg attaaatgag gtacctctaa
    36181 cataaatata ccgttatcat atccggaaac atccacctta atatcaatat ccgaaaactc
    36241 atcctctgtg ttattcgcta tacttccgta taattcgcaa tatttcacca tcggtaatga
    36301 gctgattaca ttcatacatt tatctgccat ctctctcagt tgcaaattca tatatattct
    36361 ccactttaat ttattttaag catgcgacaa atattaaatc aagagttttg cttgtgtggc
    36421 cgttttcata tataactttg aattaatctt ttatattttg aaatccccgc aactctgtca
    36481 tttatatcta tcccgtattt ttttaagatt cttaaagctt tgtaatactg ttttttatct
    36541 atttcccatt ccagttcata atcggtaaaa ccgttgtaag tattcatatc tatttcagca
    36601 tccggcatgg atacatctag ggtaacattt gctcttaaag tctccagtgt acctttataa
    36661 actacactgc tatcggatgt tttttccttt tttagatatg tcaataacgc ttcaggattg
    36721 tttttgatta tcagataatc ggattcggac agtttcattg agtattcggt tttatctttc
    36781 agacctttta attgcacacc gtcattgctc actttatatt gcaggaaaaa ttcattattc
    36841 atctttcgga tacgaacagt taaatcatcg gttattttgt aataatgatt tatctgtgta
    36901 tgtatattat gtccttttga aaaaagaatc tgatactcgt cttttgtaat cataaccttt
    36961 atttctgttt ctatatcagc agtaaatgtt ctttttatca ttaaatacct ttctttacat
    37021 aagtaatccc gccaattata gcagattgag ctcaacattt gtatttgttt caaaaaaatg
    37081 tattatttta cgtgtcactt taattcccat aattctttcc accgcttcgg aatatatatc
    37141 cagttgcttt atgtacctca aagcatgttc cttttcattg cctttttcca ctttgtcggt
    37201 tttataatca acaatagttg cgtatccgtc ttttataatt aggcaatcaa taaccccctg
    37261 cagcagtact tcatgttctg attggtattt atcgtcggta tatgcggaaa tatagcttgt
    37321 atttacaggc ataaagaaga ctttttcctg atatattgcg tccgcttcac agatatcgtt
    37381 aaataaaggg gtgccgataa atgcaagaat attgttcaaa tactcgggta ctgttatttt
    37441 tgactttata taggaaatat actcgggatt atttaattgt ctcaaatcta ttgatgctat
    37501 tatcttatgc atgattgtgc ctttttgtgc cgcacttatc ttgctatcct tttgcataaa
    37561 catgggtttt ttatgtgttt gcacacggta tcggttatag aagtcgtcaa tatcatatct
    37621 cttactctta tattttgaca attccgttac cgtaactttc ctgttaagcc ttgtttcttc
    37681 caagtaagga tattcgaatc cggtttttgc taagatattc tggtaataag cggcatcatt
    37741 taaatccctt gcctgcactg tttccgccac ttccccttcg gtcagattcc taatgatatt
    37801 ttcacagtct ttttcattta aggttttgaa tgttgcaccg taagaattgg gaagaatcca
    37861 gtcaaaagga cattttgcct cttgctctat tacattctca tcaaaacttt tatcaatgcc
    37921 ggttattatg agtttttcct tagccctggt taaggctaca tatagaaccc tcatttcctc
    37981 ggataaactc tgtttttcca tacttaatga aagtgcattt tttgttatat gtttatggta
    38041 tgtcttgtta tcgcagttta tatagtccga cccgataccc aattttctgt caataatcaa
    38101 tcttccctga gtatcggtga aattaaactt tcgggtactt ccggccaata taactacggg
    38161 gaattccagt cccttactct tgtgtatact cattatattt accgtattat ccttattgtg
    38221 acgcattatg tcgtaaataa cggataattc atgcttgtct ttactcttga gaaagtctaa
    38281 gaagccgtat aaatcgtcaa tacctttttt gtaacattcg actgccaata cataaaatct
    38341 ctttaagaga tcctgctccc tgctcacata atatattccg gactttgtca tggcgtaata
    38401 tacggttttc tctaccgtat tgttcctgga aaactttctt atatcatgaa aatcctccaa
    38461 aaacctttgt atcttagact gtaatctgtc ttcttcattg taggaaacca aagcatcata
    38521 caggtcggat ttatcttttt ttattaaagc gaattcacat tcattaaagt tatatacttt
    38581 gttctttaat atttgggcaa gataaatatc ctggtacgga ttatcaacta catttaaaag
    38641 agcatacaaa tcggagatgg ttttattact taaaaactct atacttacct cggaataggc
    38701 ggaaataccg caattattta gtatttcggt atacttatcc ccgtaattac ttaccgctct
    38761 cataagtatg actatgtccg ataactttac aggtcttgtt gttttcagtt tgtcatcata
    38821 tatcatcaat ttactgtcga taagttgctt tatttcatcg gcaattacat aagcttcaat
    38881 ctcgacatcg gacagtcggt cttccgtttc catttttgaa ttgtcaacac atattttaat
    38941 aacggtaggt ctgtaactct catcggtctc attctcatac tgtttggagc cgtacttcaa
    39001 ttcttcatct tcactgtatt cgacttctcc cagctgctcg gtcattatat ctgtgaatat
    39061 gtcgttaaca tatttcagaa tggcatttct gcttcggaaa ttatagctta aagtgagttt
    39121 agtacctaaa gaaggatcat cctcatattc ttgggattta tctctgaata ttgcggtctg
    39181 cgcatttctg aacctgtata tactctgctt caagtcccct accgcaaata cattatcacc
    39241 tctgcttatc ttatttatta tctcctcttg tatatagtta aagtcctgat actcatctat
    39301 caatatatat ttgtactttt ccctgtaaat ctcctccgca ccggacttaa gacatcttaa
    39361 tgccatatgt tccatatcgg catagtcaat cagtttagcc tttactttca tttttgaata
    39421 tacatcatcc gcctgtaaga ccgtttctac aaactttttt ataggtcgct gcataaaccg
    39481 acagtctttt ataatattgt ccgaatagtc atagaaaagt gcagctaatg aacttacggc
    39541 tttctttaac tttgttctgt aagcttttat cgcatttact gtttcttcct cgaaatcttt
    39601 ctttttttgc ggaagattcg gaaacctaat ttcatccaaa cagttcttta acttgtccca
    39661 atctttatat ttcaaagtat cttcaatacc ttcaattatc tttaaatcat tttccagtgt
    39721 cgctttatag tgttcgcctt ccggggtatt gcgtatctca tattgcacat tatctatata
    39781 tcgctttaat atatccgttt tttccggaat atggtcatat atctgttttc cgtatgcgga
    39841 ggagaaaata ttgtccttta ttaccgtctt ttcggcactt tcttttaacc attcgtgata
    39901 atcgggataa cttttaacgg tttttaatac ggaagttata aggtcggtca gtgcattgaa
    39961 attctttccg tatccgaatg tttccagcag tctgttaaaa tcggcatcct gcctgcttaa
    40021 attctgctca taaacggtat ttattgcctg taacagaaac ttatccgcaa ccgttttatc
    40081 ggcaacggaa aatcccgggt ctatgtttat aagggcggca ttctgcctta taacttccga
    40141 gcagaaggaa tgaagtgtac ttattgtagc tctgtctata tttctatact gtttgtagat
    40201 atagagatcg gaagggttgt cttgcacttt ttctcttaag gatcttctga tacgttccct
    40261 tatctcaccc gctgctgcat ttgtaaatgt tgtaaccaga agattttcac actgaacatt
    40321 ctttttctca tcggaaagta tgttaataat gcgctcgata agtgttgccg tcttaccgct
    40381 gccggcagct gcggatacca gtatattggt attcagtgtc tttattgctt ttatctgctg
    40441 atttgtccac tttaccatat tatctcatta tccttcttgg cttccacctt tcggtatcgg
    40501 tcctttttac tgtcaaaacc gcacaacatg ccgtaggggc agtatttaca tgccgatact
    40561 tttttatcca ttaaaggctt aactgcaaaa tttccgttgt ttatattatc ggctgatgta
    40621 actatggtgc tttctacact ttgaaatata ttctgctgta aatcgctgtc cagtacctta
    40681 cttttcttta tcgtgttgtt gtctataagt tcggataagc tacccgaccg gtcatcaaag
    40741 gaataccccg gcagcacctt attgatatct tcaaaggagt tatcctgact gttttccacc
    40801 ttggaatatt tattctctat cgtcatgtag tacatacctg ccaattgcgc ttttaccgat
    40861 ttatggtaag cataagcata tagggcaagc tgtatgtcca gcccttcttt tattctgtac
    40921 aaggcgatat cgttatccga tgttttgtaa tctattactc tgaagtactc tttattctcg
    40981 cctgttgcag tgtcaattct gtccacctta ccgttaatgc gtacattaac attgcccgtt
    41041 ttaaatacaa gaggtttgat tatgccgttt tcgtcaaact tggtctccag atatttcggt
    41101 ctcattttct cgtttttcat aatatccaaa gaaattgtaa tggtatccgc tgcttgttga
    41161 accgccctct tctttacata ttccaatacc gaatcgcgct tataaatatt atttgtcctg
    41221 cttatctttt ccgccgattg ctcgcagatt gcatatattt catcatatgt ataatcaatg
    41281 ttatcatcac atatcaagct catggcatca aacagtatcg agtgttttaa cgaacctaaa
    41341 ttggcggaaa aatattcaaa caccggccgc tcatcaaatt tcagcccgta tgccattaaa
    41401 aaactgtacg gacatgctac atagcgctcc aacatggtgg cgcttaagtt cattgtgtct
    41461 ccgaaaagca taccggacat ttccgattga gtaatgtttg cggtgtcgga aggtacatac
    41521 agtaactttt ccaatcgggt atctttacaa acatccccta aagaagtatt accgttaaac
    41581 aacattgaaa aggccgtttt gatattatgt gcatactcgt tttccggtat atccactctg
    41641 gttaaaactt caatattggg gaatatagcg gaaacctttg ccagccagac cgacatattc
    41701 ttgtcgccac cctcggaatc atgcagatga taggacagat atatctttct tgtaggactt
    41761 tgcataagtt catatatctg atattcctct ttgacggcat tctgtgacgg atcggttgat
    41821 atctcataac cgtattgctt aattctttca atatccgaat cattgaataa agacttggag
    41881 tcgctcaatc cgggaaacag tccttcatta actcctaaga caatcatggt atcgtaatct
    41941 tttatatcgg ctcttgctac attggtcagt ataacgcaat cttttgtgac gggagtaatg
    42001 ccaacggaag tttccataaa cgccgtcttt aagatattca tatagtattc cacggttctc
    42061 ttttcatcat ccggcataat aaccatttgg tctaagatgt ctataaaaga gtcatagcac
    42121 tgttttgcct gctccgctcc gtcactttcg gtatagtttt cttctacatc ggtcttttca
    42181 taaatacgca agttttctat aaggttgtat aaatggcgtg caaattcttc cggtctacag
    42241 ccgtatcgga ctttattaaa aaaagcgtcc agagaatcaa caattttctc tcttaatctg
    42301 ttcattctgt catcataggc attgttctcg taatcccaga cattgtgcca tgctttttta
    42361 gcggaaatac cccattttat gatgtagttc tccaatttaa gagcatcctc ttcatcaatt
    42421 atcgaataga ggttttttat aaatcttgaa gtactgtcta tgtcataatc ccagacgtaa
    42481 atatccaaga tggaaataat gaactttatt aacgggttat ccgcaatgct ctttttggag
    42541 tttatgtaga aaggaatatc gtattggtcg aaaaccgctt ttattattct ttcataggaa
    42601 gaaacatccg ataccacgca aagaatatcc ttgtatctta tccctctgtt ctccacggca
    42661 ttcattatcc taatagccgc atattccgct tcttcgtata tatcatatgc cttaaaaatc
    42721 tcaatatcct ttactgctcc tttgcttatt tcatttcttg aacgaaacag attggaaaaa
    42781 agaaagtcca atgactgaga ttgcactgtt tcttcggtat atgtcttatc ccgctcctca
    42841 ataaaagtag ttctctgttg ctttgcctgt tctttcagtt tgtccatggt gcataacggt
    42901 atggtgaata tatcgctgtc aggaccgctg attccgtaat ctatgcataa agagatgttt
    42961 attctctcgg ccttattcat aaggcttttt atgacatcaa actcttttgc ggaaaaaaat
    43021 gaaaacctgt caaaccagat gatactgttg tcaaaggtcg ttgtattgtt aatatgctcg
    43081 cataacatat cataagaatc cgtttggtcc gctaatccca tctccttcat acttctcatg
    43141 taaaactcat atatagcggc aatttcggca aattttgttt ttgtcgcata ggaaaaatca
    43201 ccgttctcac ttatacgcag tatatcctca taacttatat tatttgctct aagctctttt
    43261 atggtctgca ttgcaagaac ggaaaaaccg ggatattttc tggatttttg ataataatcc
    43321 agggacagtt cttctttatt cagtaaagaa taaagtaaca tggtctttcc ggtatcgttt
    43381 acggtatcgt ttctttcgat atactccaaa tcaaacagtc tttgcgtcag acggctgaaa
    43441 gtcatcactt cggctttcat aaagcctctg tcggatgcaa gcgtctcctc atagtatgat
    43501 gaaaactgtt ccggaacaac tacaataagg cgatatttat ggtctttttc tatcgcctga
    43561 ttgatttctt ttataaatgc ttttgttaaa gctttttttt tcggtccgta taatattcgt
    43621 aaactcattt acacagttga tatgctatct ttgccgcaac attcgagtta ttcaacacca
    43681 gctttatatt tgcatccaga ctttcacccg cagtcagctc ttttatttta tctaaaagga
    43741 acggcgttat ctttttacct ttaatacctc tcttatccgc ttcggctaaa gcctctttaa
    43801 taatattatc gatatatttc ttatccatag aatactgttt gggtatggga ttggctacaa
    43861 ccacccctcc ttgcagtcct aagccccatt tagcccttat tatatctgca gcatgttcag
    43921 catcgtttac tacataatcc actccgaaac cgctgtcatc ggtatagaaa gcgggaaact
    43981 tatcggtctg ataccctaag accggtacac ccatagtttc cagatattcc agtgtaagtc
    44041 cgatatccag aatacttttc acgcccgcac atacaacggc aaccgaagtt ttggccagtt
    44101 cctgaagatc cgcggatatg tcaaatgtat gagacgcctc tctgtgtact ccgcctattc
    44161 ctcctgttgc aaaaaccttt atacctgcca atgcggctat tatcattgtc gcggccacgg
    44221 tagcggcacc gtcctgtttc tgtgccacaa cagcaggtaa atctcttctg gacatcttta
    44281 atatgccttt tgcttttgcc agatactcaa tctcatcatg acttaatccc gctttaagtc
    44341 tgccgtcaat aaccgctatt gtagcgggaa cggcattatt cttacgtata atgtcttcac
    44401 acatcagagc cgtttcaaca tttttcggat atggcatacc gtgacttatt attgtggatt
    44461 ccaacgccac tacgggttgt ccttcttttt tcgctttttt cacttcttcg gaataatcaa
    44521 tataactaat cattttttcc cgtttccttt aacattttta atatttttct ctccgttata
    44581 tccttgctta ccgcaccggg actggataaa gtaataaagc tcattatgct tccaagcctc
    44641 atacagtgca aagtatcata acctttttta attccgtacg ccaatccggc tgcataagaa
    44701 tcgccggcac cgttcaccga aacaattttt tttacatcaa ttgcatctac atgaacaact
    44761 tgatcattct cgtagccggt actgccgtct gctccttctg ttacaaaaat cctgttttta
    44821 tactttttag acattctctt cccgttatca acagtatact ccacgccgta tagagcggaa
    44881 aactcctgcc tgttaagttt aacggtatgt atatctgaaa gacacttttt cagtttaata
    44941 acctttgcgg aagaaaccgc ctcggctatt ataatcttat ctctatattt atctgcaatg
    45001 tattcaatca taggcttgga aagattacag tcaataacaa tacaagttgc attgacaata
    45061 acctcattac acttatctat atgtgataac ggcatattca ttattgcgga taaatcgcat
    45121 actgcggcca ccatttcacc tgtatgatcc tccaatgaaa gatatgtacc ggtagaatat
    45181 tccttcgttc cataggaata tttagtggaa atacccaatt tcgcacagga agtctttaac
    45241 atatcggcca gatggtcatc agaaaaggta gttatgaact cacaggaaac acccaaacga
    45301 accagatttt cacatatgtt ccgggcaacc cctccggaag ttaatgtaac cttccctata
    45361 tttgattcat tccttacgac atccttaacg gcttttccag taatatcggc atttatgcct
    45421 ccgataatca caatatatcc gttttccata tgtatcacct tccaatgata ataatatcac
    45481 atcatcgggg ctgatactat cttttataat tgacaacaag ttcggtttac ggtatatttt
    45541 gaaacgcaaa gttcttttgc tggtgtggct cagctggcag agcagctgat tcgtaatcag
    45601 caggtcgccg gttcaaatcc ggccaccagc tccaaaaatc ctcagtttat atgctgagga
    45661 ttttctttat acacggctca atcagaaaca gaactacaaa accgatatgt aaaattaata
    45721 aatatttgtt tttacctata aatccagatt aatacgcttc ttttctcttg actgcttata
    45781 caatacgaac ttattaccta ttacctgcac aacttcggca cctattgcct cggctatatc
    45841 gtttgcaacc tgcaaaggct catactcaca gcttttcagc acagttgtct ttacaagttc
    45901 acgagcctct aaagcctctt tatacataat aagagtgttt tcgcttattc cgtctttgcc
    45961 cagttgtata atcggttgta ttttctgtgc cagtcctttt aaatatgctc tctgcttact
    46021 tgttatcata tatatacctc ttatctgaaa tattcgaatt caaaatcatc tattaccact
    46081 aaatcttctt cctgtatgcc cgccttttcc aattcttcaa ttacattctt ttctcttaat
    46141 tgtctttgga aatatctcaa ggattcggta ttgtaaatat tgatggagtt tacaagcttg
    46201 gttgcccaag cgccttctac atagtatatg ttcccttctt tacgaacggt gaaacgctcg
    46261 tcttctgcat gttcatatac cgcatattcc tccgcttccg gcataataac cgtatcgggc
    46321 agtttactga gtaattccgc acatcttttt accaatatat cggttccttt atttgtagcg
    46381 gcggaaatct ttaatacttc atagcctttt tctttaattg cggcttcaat ttcttccgct
    46441 ctctttatct gttcatcggt tgtacataaa tcggtcttgt tcatcacaca tatctgatga
    46501 tgctcataga gccgcggatt atacttagcc agctcattgt tgatattatc aaa
//
LOCUS       DAOSOM010000014        44812 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_883_length_44812_cov_6.251916, whole genome shotgun sequence.
ACCESSION   DAOSOM010000014 DAOSOM010000000
VERSION     DAOSOM010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 44812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44812
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_883_length_44812_cov_6.251916"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1361)
                     /gene="xylB"
                     /locus_tag="PLF14_04770"
     CDS             complement(<1..1361)
                     /gene="xylB"
                     /locus_tag="PLF14_04770"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HOT70641.1"
                     /translation="MNYLLGIDIGTSATKSVLFSEDGKAYASASYEYPLYQPNPGWAE
                     QDPDDWWDAVCFTCRKIIADTGIDRGLIKGVGISGQMHGLVMLDEKDRLLGRSIIWCD
                     QRTEKQAEYLEDKIGRDRLISITANPAITGFTAAKILWVMQNRPDLYKKAKKILLPKD
                     YILYMLTGEFSADVSDGSGMQLMDVKKRVFSKELCDALEIDTSLLGYMHESADAAGKV
                     TKKAASLTSLKEGTIVAAGGGDQACGAVGNGIVKEGIVSSTIGTSGVVFAHTDKLHID
                     KLGRIHTLCHAVPGCYHVMGVTQAAGLSLKWFRDNFCKEEIDAAAKKGIDPYVIMDKM
                     AEEIAIGAGGLIFLPYLMGERSPHLDPFCSGVFFGITPTHGKAHFIRAILEGVVYSLK
                     DSVCILREMGIDVSQVRVSGGGAKSPLWKTMQADIFESDIATVVNSEGSALGAAILGG
                     VACG"
     gene            complement(1375..2061)
                     /locus_tag="PLF14_04775"
     CDS             complement(1375..2061)
                     /locus_tag="PLF14_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004167504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOT70642.1"
                     /translation="MSDAIYDRTIRLIGEQAMKELRQKKVAIIGLGGVGSYVLEALAR
                     AGIGHLILADYEKIEESNINRQILALHSTVGKYKTEVALERVKDINPDIKVTVHNVFV
                     SKDNIKDIIPLDCDYVIDAIDTVSSKIDIIVYAKKNGINIISCMGTGNKIDNTGFKIA
                     PVEKTDTCPLARKVRKILSSEGITGVDVLYSVSKNQVSDPNLISGISYVPSTAGLIIA
                     GYVIRKLINM"
     gene            complement(2058..2927)
                     /locus_tag="PLF14_04780"
     CDS             complement(2058..2927)
                     /locus_tag="PLF14_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOT70643.1"
                     /translation="MRLDVYLTKYIEDYSRSFIQKIIKEGQVTVNSLVRNASYSVKNN
                     DVIKVIMPDILPSSIQPQNIPLDILYEDDDVIVINKKRGMVVHPATGNYSDTLVNALL
                     YHTKNSLSDINRVERPGIVHRIDKDTSGVILACKNNYSHRVLTESFARHDVKREYIAI
                     VKGYPNSDKGLINAPIARDRLNRLKMAVDSDGKKAVTHFEVLEKMHGASKIKAMLETG
                     RTHQIRVHMAFIGNPLLGDDIYGGKDKRFNKGQLLHAYLLGFIHPVKKEYMEFTAPPD
                     DYFKNAMKVLVNT"
     gene            complement(3023..3502)
                     /gene="lspA"
                     /locus_tag="PLF14_04785"
     CDS             complement(3023..3502)
                     /gene="lspA"
                     /locus_tag="PLF14_04785"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002510922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOT70644.1"
                     /translation="MLRRINFKNILWLFLSIMIVAADQLSKFFIVSNMQLGDSIKIID
                     GFLYITHTRNTGAAWGIFGEHTEYLAIFSVVATIALIVLLLYIKRAFGAFSLALIIGG
                     AVGNAIDRIRLGWVVDFIDAYIFGYDFPAFNVADSAITVGVFLLIIYMLFIHREMSP"
     gene            complement(3486..4271)
                     /locus_tag="PLF14_04790"
     CDS             complement(3486..4271)
                     /locus_tag="PLF14_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOT70645.1"
                     /translation="MRVLFIGDIYGKTGRVVLKNNIAYLKEQYAPDYVIANGENTAGG
                     KGITPTLLQELLSMGADVVTSGNHIYARKEILPAFSDENIKLLRPLNYAKTCPGKGYT
                     ILKNGCSPLCVINVSGRAFMEPCDNPFYAVESVLDQVKKEARNIVVDFHGEATSEKAA
                     FAWYFDGRVSAVIGTHTHVQTADERILPFGTAFITDAGMTGPHEGIIGASRDTVIEKF
                     LTGMPAYFTSQEGRGQLNAVILELCEKTGKAINIERINNVEKD"
     gene            4395..5846
                     /locus_tag="PLF14_04795"
     CDS             4395..5846
                     /locus_tag="PLF14_04795"
                     /inference="COORDINATES: protein motif:HMM:NF013444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT70646.1"
                     /translation="MKTHKKGILNRIKQPDIQKSIPFHMPGHKRNVNFNKHLNKIGIR
                     YDFTEIPQLDNLQAPEGIIKNVMDKAQLLWNTKKSFLSVNGSTCGINASIATLADLKD
                     KIIVARNSHKSVYNAIELLDLEPVFIAPAFNNKLNISGSINPETVYNAIFCNPDAKTV
                     LLTSPTYDGVISDIKAICDISHKMNIPVIVDEAHGAHLHLSNCFGNSAISANADIVIQ
                     SLHKTLPSLTQTAIAHICTDRIDVNKFQRYLSVFQTSSPGYPLLSSIESCIDLLLNNK
                     DKLYSDWIKNIDYIDNRISELNNIKAFYHGLSKNEVYENIYAYDRSKLLISTDNLNIN
                     GKQLFDILMQDYGIICEMYTNSYVLAMLSMCDKKSSVRKLCNALEEIDLTYPKNNKNT
                     IKNTQTYDIPKRCMSIREARNKKSMSIPLSEAQGHICAEYIWAYPPGIPYLIPGEKIT
                     REITDILKEAIDNGIDLKTDNNDNEGCITVLCN"
     gene            5914..6069
                     /gene="scfA"
                     /locus_tag="PLF14_04800"
     CDS             5914..6069
                     /gene="scfA"
                     /locus_tag="PLF14_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015563834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HOT70647.1"
                     /translation="MEKTKRIKTIETRNLVSSIKNGGCGECQTSCQSACKTSCGVANQ
                     KCENTDK"
     gene            6146..7516
                     /gene="scfB"
                     /locus_tag="PLF14_04805"
     CDS             6146..7516
                     /gene="scfB"
                     /locus_tag="PLF14_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HOT70648.1"
                     /translation="MIHQYKLNGYNIVLDVYSNSLHVTDNLAYDIIALYKDKSKEEIV
                     EIIKNKYPDLTNNDICECLSDIEQLERDNKLFSEDVYKDVPVNKISEGNIVKALCLHV
                     SHACNLNCSYCFASQGNYKGKRALMSFEVGKQALDFLIKNSGNVRNLEVDFFGGEPLM
                     NLDVCKQLVQYARSIEKQHNKNFRFTLTTNGMLIDDDVISFANQEMDNVVLSLDGRKH
                     VHDRLRKDINGNGSYDVIVPKFKKLVDNRTKGTYYIRGTFTHHNTDFTNDILHMADLG
                     FKELSMEPVVCSPDDEYALNEKDLPVLLEQYELLAKIMLEREKAGKGFTFYHYMIDLN
                     HGPCIHKRLSGCGSGREYLAVTPWGELFPCHQFVSDSRYSMGDVWKGVTNTGLRKKFE
                     LCNTYTKEECKNCWAKLYCSGGCAANAYHATGNINGTYEYGCILFKKRMECAIMLQIA
                     RAETAD"
     gene            complement(7662..9218)
                     /gene="rny"
                     /locus_tag="PLF14_04810"
     CDS             complement(7662..9218)
                     /gene="rny"
                     /locus_tag="PLF14_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOT70649.1"
                     /translation="MSLREIIGLVIIAFLIIFSIVMFFLGIRHRKKVAEKEIGSAESE
                     AKKLILQGEKNAEERKREILIEAKEEVHKSRVEMERELKERRKEIQRMESRVIQREEA
                     LDKKVASVESKEEILNKKMSDIDSKQKEIEQIHQDKLTELERISALTVEEAKTYLLKT
                     LEDDIKHEKVLLLKEYEAKTKEESENLAKNIISSAIQRCAADYVAENTVSVVELPNDE
                     MKGRIIGREGRNIRALETLTGIDLIIDDTPEAVVLSGFDPIRREVARIALEKLILDGR
                     IHPARIEDTVNKAKKEIENTIKIEGDKVSFEAGVHNLHPEIIRLLGRMKYRTSYGQNV
                     LKHSLEVSLLTGIMAAELGLDVPLAKRAGLLHDIGKSIDFETEGSHIDVGADIARKYN
                     EHPVVLDAIMSHHGDKEPESAIAVLVQAADAISAARPGARRETLEAYVKRIEKLEEIA
                     NSFEGVEKSFAVQAGREIRIMIKPEKMDDDDMVLICRDIVKRIENEMEYPGQIKVNVI
                     REVRVVDYAK"
     gene            9508..9756
                     /locus_tag="PLF14_04815"
     CDS             9508..9756
                     /locus_tag="PLF14_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010296157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOT70650.1"
                     /translation="MKSAGVVRKIDELGRIVVPIELRKSLNIGKREAVEISMEDDCIL
                     IRKFEPGCCFCGSTDDLEVYHGNKVCNKCIKDLSSATE"
     gene            complement(9739..10578)
                     /gene="rsmI"
                     /locus_tag="PLF14_04820"
     CDS             complement(9739..10578)
                     /gene="rsmI"
                     /locus_tag="PLF14_04820"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOT70651.1"
                     /translation="MLYLVATPIGNLSDISKRAIETLTNCDYIASEDTRHTLRLLNAL
                     GIKKKLVSYHQHNMESSGQSILKDVMNGMDVCLVSDAGCPAISDPGNELVKLMHLHNQ
                     KVTVIPGPNAAISALMISPFDTSSFCFEGFPPVKISEKRQFFEKIRNESRTCIVYEAP
                     HKLIKTIDVARQVMGGNRRVSLVKEITKIYETVVVDSLDGIAEYLEQNPPKGEYVLVF
                     EGNTDTNNETENPCPHSQRDIAEKYEELIKNGFSRKDAMRKVAVLFNMSRNDVYAILF
                     GSR"
     gene            complement(10581..11312)
                     /locus_tag="PLF14_04825"
     CDS             complement(10581..11312)
                     /locus_tag="PLF14_04825"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HOT70652.1"
                     /translation="MLHEDERLDDLQLEGLKLIQNKKQFCFGTDAVLLSSFANVRSTS
                     SVIDLGTGNGVIPLLLYGKYKPEHITGIEIQKDAYDLAVRNIQLNNLEDKITVINGDI
                     RNIKEYFKTGSFDHVVTNPPYLKTGTGIISMADTMAYTRHEILCTLEDILKATKYLLK
                     TKGIFTMVHRPQRLDEILLLMKTYSVEPKTIRFVHANVKSPAILVLIQGLKDMNPSIT
                     VEPPFIIHKPDGSYTEELNILYGRN"
     gene            complement(11431..12057)
                     /locus_tag="PLF14_04830"
     CDS             complement(11431..12057)
                     /locus_tag="PLF14_04830"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HOT70653.1"
                     /translation="MKIIRFKFRKTEKVKYVAHVDMINAFSRALRRAELDVYYTRGFN
                     PSIRMVFALPLPVGVSSDSEYVDIWFNTEYECRYALNKFSQALPDGFELLEAYTIEKS
                     TLMEDVGSAIYGFRVNCNGNLKKALDKLISLDSIEVEKSNKKGLVNILPYLYKIDYYD
                     ENRFTVLCKAGAKGNLHPRLLLKALEKYTGIQVEELSINRNKLFLKVQ"
     gene            complement(12038..13801)
                     /locus_tag="PLF14_04835"
     CDS             complement(12038..13801)
                     /locus_tag="PLF14_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HOT70654.1"
                     /translation="MIDLINVEKPARYTGGEFNEIVKDHDKMQSSYAFCFPDIYEVGM
                     SHLGMRILYHVINRRKTTVCERVFAPWTDMEQLMRANKVPLFSLETKKPLKEFDIVGF
                     TLQYEMSYTNVLNMLDLADIPIRRSIRTDEHPIIMAGGPCAANPVTLEDVIDIFLIGE
                     GEEVMNEVLDLYEDGKSAGQSKEDFLKESVKIEGVYVPKYHDFDKKVIKRIIKNLDSV
                     SFPTDSIVPYMNIVHDRASLELFRGCIRGCRFCQAGYIYRPVREKKASTLIEQTKRIL
                     ADTGYNEIGMVSLSTSDYTDLKTLCDGMLEHTEKRMVNLSVPSMRIDNFSIELMNRIQ
                     KVRKSGLTFAPEAGSQRLRDVINKNITEEEILTSAKIAKSGGYDNIKLYFMLGLPTET
                     DEDVIAIAELAGKILDIFGRKSRINITVSTSFFVPKPFTPFQWCPQITVEEMIRRQRL
                     LRSHIKEKKIKYNWHDFNTSVVESAISRGDRQVGQAIIKAWEKGCKFDSWNELFDMEK
                     WEQAFSECSLDIYECASKQIALDEELPWDFIDIGVKKSFLKNEYQKALREETTENCRE
                     HCNNCGIAKYRCGVCFEDNKI"
     gene            complement(13808..14080)
                     /locus_tag="PLF14_04840"
     CDS             complement(13808..14080)
                     /locus_tag="PLF14_04840"
                     /inference="COORDINATES: protein motif:HMM:NF018633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HOT70655.1"
                     /translation="MDDERDNIIVMEDDDGNPIEMMVLEMLDYDDRTYVLMQPADEAE
                     EDAYIFEYEEDKDDAILTPVEDEQLLDELFELFMEKMDSDFDEEEE"
     gene            complement(14127..14552)
                     /gene="ruvX"
                     /locus_tag="PLF14_04845"
     CDS             complement(14127..14552)
                     /gene="ruvX"
                     /locus_tag="PLF14_04845"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOT70656.1"
                     /translation="MVIIAIDYGDKRIGVAKSDPLGLLAHGVTTIEWNSDINRPLDIL
                     EGIIDEYKASGIVVGLPLNMDDSEGEKAMQVRTFASMLKERCKLPVILFDERLSTVEA
                     HDIMHMQGKKTGKDKKRVDMVAACVILQSYLDEMNKKKK"
     gene            complement(14558..14809)
                     /locus_tag="PLF14_04850"
     CDS             complement(14558..14809)
                     /locus_tag="PLF14_04850"
                     /inference="COORDINATES: protein motif:HMM:NF003997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HOT70657.1"
                     /translation="MDRGSTINLDFLKGHHAETREILTLVFSAMTEKGYDPVNQLVAY
                     LMSGDPTYITSHNGARNAIRKLERDEIMTELIQYYFASK"
     gene            complement(14915..16237)
                     /gene="mtaB"
                     /locus_tag="PLF14_04855"
     CDS             complement(14915..16237)
                     /gene="mtaB"
                     /locus_tag="PLF14_04855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOT70658.1"
                     /translation="MMKTYSIATLGCKVNQYESATIASSLDAMGFQYKKFPDICDIYV
                     INTCTVTAMSDRKSRQMISKARTTNPDSIIIATGCSAQANAQAMIKSGADIVLGNNEK
                     SSIDTILSSYLKEKTGKLTVVSDIFREKEYIHGCFPDKWERTRGYLKIQDGCDNFCSY
                     CIIPYVRGRARSRDIDDIVKEARYMASKGIKEVVLTGIHLDSYGKDKGNLSIIDVIAK
                     INDIKGIERIRLGSLEPKIITETFVEEIKKYDKLCPHFHMSLQSGSDAVLSDMRRRYD
                     SEMFLSAINMLRNNIPNLEFTTDIIVGYPTETEEDFNKSLEMVEKARFIKVHVFKYSK
                     RPGTAADKLKPLNGKTLNERSEKLINYASEISKSRLSGYLGKTLRILIEEGEDGFITG
                     HTANYIKVQAPTDNNVTDDYINTFCDVLISGLTNDGLLGTVVDKKTVR"
     gene            complement(16242..18581)
                     /locus_tag="PLF14_04860"
     CDS             complement(16242..18581)
                     /locus_tag="PLF14_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOT70659.1"
                     /translation="MAQKKRTSVTQKKTEKPETSTKNNVLAKEITGIVIIALALLAFI
                     CIFFTSNSGAFGRPFKQVTAGLFGWAHYLIPIVILLFGVYVALAGRVKWLSRKTMYLI
                     ILILMVPCFFQIAYLSKNMADIAVDMNVWKFIAHMYSIGISQVYNGGVFGGIIMTPIV
                     AFFGVTGAYIVAVTASLTLLIYITNKSFLDFLVKIKNLFVKFFHWMKDAFKPIEEQEE
                     EPEPVKSKKQQYVQGTINDIAEPVKETISREPANIIINKSKPVKETKSTVPKYTGPII
                     IKKEEPVKEASVTAEKPQIKGEYVFPPLSLLDSYVDSGNTDNHEQIARKLENTLLSFG
                     VEVTVTNIMCGPTVTRYELQPKAGVKVSRIVALADDIALNLASRDVRIEAPVPGKSVV
                     GIEVPNTKKSVVSLKEVIASKEFQSSKSILTIAIGKDIAGNCTVADVRRMPHMLIAGA
                     TGSGKSVCINCIITSILYKATPDDVRFIMIDPKKVELINYNGIPHLLIPVITNLKKAA
                     GALNWAVEEMTNRYDMFNKRGVRDINTYNDSVKGDPQLKKLPNIVIIIDELADLMMSN
                     PGEVEESICRLAQLARAAGIHLIIATQRPSVNVITGVIKANIPSRLSFAVVSQVDSRT
                     ILDMGGAEKLLGNGDMLFHPMGLSKPVRMQGAYISDAEINKVVDFIKQRTSATYDEDV
                     VENIEKADKKGADGDLENEVDELLPAAIDLVVEAGQVSVSYLQRKLKLGYGRAARIVD
                     QMELRGIVGPFEGSKPRAVLISRDEWAEMQMNNPDLDIN"
     gene            18709..19716
                     /gene="asnA"
                     /locus_tag="PLF14_04865"
     CDS             18709..19716
                     /gene="asnA"
                     /locus_tag="PLF14_04865"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HOT70660.1"
                     /translation="MSVTYIPKGYRSKLNLYETQLAIGTLKRIFEEHLCKAINLKRVS
                     APLFVRANSGLNDNLNGIERPVAFETKEGGMQAEIVHSLAKWKRLALHKYGFPAGEGL
                     YTDMNAIRRDEQMDNIHSIYVDQWDWERVITKEERTVAFLKQIVSDIVSAICDTYERM
                     RDIFPALDTDISREVFFITAQQLEDMYPDLTSKEREDAIVRQHKTVFIMQIGKTLKSG
                     QKHDGRAPDYDDWELNGDILMYNEVLDSALEISSMGIRVDSESLARQLKLSGCEDRKE
                     LMFHKMLLNNELPLSIGGGIGQSRLCMLLLKKAHIGEVQVSIWDRDTIEACKEAKVIL
                     L"
     gene            complement(19718..21775)
                     /locus_tag="PLF14_04870"
     CDS             complement(19718..21775)
                     /locus_tag="PLF14_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOT70661.1"
                     /translation="MEGILDIFASNVYNDQVMKNELPAYVYQSLKKTIEQGGPLDDSI
                     ADIVAESMKNWAISKGATHYTHWFQPMTGMTAEKHDSFLMIRNNVPVLELRGKELIKG
                     EPDASSFPSGGLRATFEARGYTTWDATSYAFVKDKTLYIPTAFCSYGGEPLDNKTPLL
                     RSMDALNKQALRIVRLFGNETASRVTPFAGCEQEYFLIDKKQYDKRKDLLICGRTLFG
                     AKSPKGQELNDHYFGNIRERVQNFMRELDVELWKLGITAKTEHNEAAPAQHEIAPIYT
                     TVNIAADHNQLIMEMLKKVAEHHGLACLLHEKPFENINGSGKHNNWSIATNEGEMLLK
                     PGKNPARNKRFLLILVAVLRAVDQYAGLLRQSIATAGNDHRLGSHEAPPGIISVYLGA
                     ELTEILEDIANDRKVHNRTREKFHISETLPVLFKDTCDRNRTSPFAFTGNKFEFRMPG
                     SSCSVSDANTVINTIVADSFKHFADILEKAEDFDKEVDKLIKETVNKHGRIIFNGNNY
                     SEEWVEEAKKRGLPVLNSTPEAVDCLVDKKNVQLFERFGIFSEADLVSRHEIKMENYC
                     KIISIEARTMLEIAKREILPAAMDYARTVYGSVSMKTDIGLDASTERELATYISDNIT
                     GLSKCISKLEKVTEQSVDAYAFKDHVVPAMKELRHFADSLECVVAKSYWPMPAYSDIL
                     YSV"
     gene            complement(21841..22944)
                     /locus_tag="PLF14_04875"
     CDS             complement(21841..22944)
                     /locus_tag="PLF14_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT70662.1"
                     /translation="MEKHIQKIHDFYNFKKDAAIFQKEFGFYVKGKWKEQGWINDDTD
                     TDELFGFDKSAVFFLGRCGWCEAPLVPSFERKIISTTDECEIEQDNAGRQVLYFKGRR
                     SGFMPTYLEHPVKDIRTLEENILWRLNPDDPKRFEGLEERMSKAAESEKQGYIICANI
                     IGGYMYLRSLIGPEDLLYKFYDDPELIHKCMEIWFDVMDKTCEVHQRYVTVDEVYFGE
                     DICYNHGPLISPDMIKEFLFPYYQQLIANVKKRQSDKNRKLHFQLDTDGNAVTVIDLY
                     KSIGMDYMSPFEVASGCDVVEVRKKYPDLLISGGIDKRILAAGKDAIDKEIDRIMPYM
                     KKHGGYIPTCDHGVPEEVEFLDYMHYRKRMLEY"
     gene            complement(23014..23955)
                     /locus_tag="PLF14_04880"
     CDS             complement(23014..23955)
                     /locus_tag="PLF14_04880"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT70663.1"
                     /translation="MGFEIILSIVVFVVVILFILSFIAGLFMFNITMIPGSPRNYFNK
                     KEEDIDEQVKEFFASSEQWVKENTTSVWSLTSFDNLNLKAVFVENKGSHKWVIPVHGY
                     TSRGWHMAQFAKQYYKAGFNILMPDLRGHGDSEGQYTTMGYYESVDVIDWIKEIIKKD
                     PDALIVLHGISMGAATVMLTTGEDLSNHVKCAIEDCGYTSVWDIFSYNMKRFFKLPAF
                     PVLYVSNIISRARARMDFKQASPLNAVKKSKTPTLFIHGDADELVPFYMLEPLYDNAP
                     CEKDILVVKGAEHGAASAKEPDVYYKKVSEFISKYLN"
     regulatory      24124..24299
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            24369..25367
                     /locus_tag="PLF14_04885"
     CDS             24369..25367
                     /locus_tag="PLF14_04885"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOT70664.1"
                     /translation="MKKYNLAIVGATGMVGRTFIKVLEERKLPIKNIYFFASARSCGN
                     TITFSGKEYIIEELNEHSFDRLIDIALFSAGSDLSKIYAPVAAQKGVVVIDNSSAFRM
                     DPTVPLVIPEVNKEDIAKHNGIIANPNCSTIQALVAVKPLHDAYKVKRMVVSTYQSVS
                     GAGKAGCDDLENCLKGLPPAKFPYSIADNCIPHIDSFLDNGYTKEEMKMVNETQKMFH
                     TKDILITCTCVRVPVMRSHSESINLEFEKQFDIKDVYKILNNAPGVKVVDDLCNAKYP
                     LAAEAADTDWTYVGRIRRDDTVESGLNIWVVADNVRKGAATNAVQIAEELIKLWEK"
     gene            25369..26241
                     /gene="dapA"
                     /locus_tag="PLF14_04890"
     CDS             25369..26241
                     /gene="dapA"
                     /locus_tag="PLF14_04890"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOT70665.1"
                     /translation="MLFKGAATAVITPFNKKGIDYDNFALQIDNQLKAGIQALIVCGT
                     TGEASTMTKKERYDAISFVVNKVQKTVPVIAGTGCNDTMHSVQLTKDAYKAGADAILV
                     TAPYYNKTSKKGLIEHFSVISESVPVPMILYNVPTRTGMNIEPDVMVHLAKNCNITGV
                     KECNLSQVADIIQTAPENFTVYSGDDSINLQALSLGAMGVISVMSNVLPAHVVKMNKL
                     FLQNKIKESRELFYKTKNLVKLLFIETNPIPVKKAMQLAGYDTGLLRLPLVEMEEKNA
                     NLLKEELINLGVIK"
     gene            26238..26990
                     /gene="dapB"
                     /locus_tag="PLF14_04895"
     CDS             26238..26990
                     /gene="dapB"
                     /locus_tag="PLF14_04895"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOT70666.1"
                     /translation="MNILLVGCYGKLASAIIKACSGKEDMNIACGVDLSVKDNPHFPV
                     FTAANDIPSSMDIDAVIDCSHPSGAVSTVRFCKKYKKPLVYATTGLDKEQMNLLHELS
                     KHVAVFQSANMSLGVNLLIKLCKQAATFLGNDFDIEIIEKHHNQKIDAPSGTALKIAD
                     EINQSLNNQMTYVYDRQSKREKRDKKEIGIHAVRGGNIVGQHEVIFAGQDETITISHG
                     AFSKDIFAQGALRAVLFIKDKPAGLYGMDDLL"
     gene            complement(26997..27842)
                     /locus_tag="PLF14_04900"
     CDS             complement(26997..27842)
                     /locus_tag="PLF14_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70667.1"
                     /translation="MSDKKKVNYQEQKPTETKFMKFMEILKTRFWNIAKYNMICVGIY
                     ALFIVFYAFFVSQIYFPIDVSDYQQGYSNFGELWAILILRIPYTLVFFTIPVVAFGPA
                     VSGLAVVFRSYVRRDDSFSSDFGDGMKKFFWKSLAVTAISTVITVCLGIAARFYQLNL
                     QAGFWKTALLLIIGGFSLLFIMMHMYIYQILVEYNLSIWKLYRYSYVFAILRFFPNLL
                     IIAGIAALTILPFKIHILFGAVVMATVLFGLSSYIINFFTWPALEKHLDPIMKEQRIN
                     KVKLY"
     gene            27986..28068
                     /locus_tag="PLF14_04905"
     tRNA            27986..28068
                     /locus_tag="PLF14_04905"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28020..28022,aa:Leu,seq:gag)
     gene            28150..28806
                     /locus_tag="PLF14_04910"
     CDS             28150..28806
                     /locus_tag="PLF14_04910"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT70668.1"
                     /translation="MTIRKMRTELGDTQSEFAARYNIPFRTIQNWETEVRKPPEYIIE
                     LLRQRVRADLANRRTVNLPEYCPEKRTLPKRSDFIGSLSWLKAVQACLGDDIVFALDQ
                     ALMCQGNFGGRSDEYSVWVYGNDAVTDYNGVIVIGNRVSPYDVESKNGFRYTNLSRTI
                     SDAFANESILDMQGVTEAVSRYYYSNNEKFDGISVAPEYQERFERLAREAIEYYKTKR
                     "
     gene            29038..30057
                     /locus_tag="PLF14_04915"
     CDS             29038..30057
                     /locus_tag="PLF14_04915"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HOT70669.1"
                     /translation="MTRKSILFVQVGKAELVDIPLPELKTNEVLVRTAISTISSGTER
                     ANLCGDLNVSPMQPTLTVAKFPRSSGYSTSGTVEAVGAAVTKVAVGDRVAMCWSCHTD
                     LNVLSENLVYKLDDNISFETAALLHIGTFPMAAIRKTRLEFGEPAIVMGQGILGQIAV
                     LQLKVAGASPVIAVDLVKERRDIALNLGADFALDPTDSEFVNNVKAICPLGVPVAIEV
                     TGVGKGFDQVLDCMAQFGRVALLGCTRNSDFTIDYYRKIHRPGITVVGAHTMARPEAN
                     SYPGYWTRTDDMEAQLRLIKYGRLSYDGLVKDTRSPVEAPEVFNRLANDPHFPITQFD
                     WRRLL"
     gene            30054..31052
                     /locus_tag="PLF14_04920"
     CDS             30054..31052
                     /locus_tag="PLF14_04920"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT70670.1"
                     /translation="MKPFRIAQIGTSRWSHGNECFRALKNNPDVFEIAGYCFPENEQE
                     KFPKQMNIFEGYRELTLDEILNDPTIVAVAIETEEKYLTKYALSVVRHGKHIHMEKSG
                     GLDSAEFEQVATIAKTNQTVFHTGYMYRYNPYVQELLEQIKCGELGEIISVEAQMNCY
                     EPLECRKWLEDYPGGMMFFLGCHLIDLIYTIQGQPKAIIPLNKSTGLDGVTCKDFGMV
                     IFEYDKGVSFAKTSAVEGGGFERRQLVVCGSKKTVELHPLERHIENRLLTTGRYIREN
                     STLWNTVHEQEFAEPFDRYSPMMLSFAQMVRGEKKNPYDYDYELTLHQLVIKASGG"
     gene            31763..33349
                     /locus_tag="PLF14_04925"
     CDS             31763..33349
                     /locus_tag="PLF14_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017426026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOT70671.1"
                     /translation="MERNISAIISEGKTFIGIEFGSTRIKSVLIDDAFNVLASGNFDW
                     ENKLEGGYWTYGKDEIIEGMQISYRNLAADVYEKYGVKLIKTAAIGISGMMHGYLAFD
                     SEDNLLVPFRTWRNITTGVAAKKLSETLSFNIPKRWSISHLYQAILDKEPHVDKIDHI
                     NTLAGHIHYLLTGKKVVGIGEASGIFPLSGNGYNKEMLKKTEELFLASGYSLKLEKLL
                     PSVKKTGETAGYLTERGAKLLDPTGTLQHGIPLCPPEGDASTGIVATNSIRPGTGNIS
                     AGTSIFATVVMKSLLPINNPEIDIMSTPDGYPTAVIHCGNCTGDIDAWMHIFQEVLKA
                     TGSSVEKSKLFETLMNKAMEADADLGGLMNFNYISGESITGIKPGVPLFLRQPGARFT
                     LANFLQTHLFSALATLRIGMEYFFKEGVKFNRIICHGGFFHSQAGAKAMAAALDSPVY
                     LYKTASLGGPWGMALLAAYSQSEESLTLADYLDNKVFKNFELIQVLPDRDTVTSFVSF
                     LEKYRKFLKVEADASRCFDE"
     gene            33342..34040
                     /gene="araD"
                     /locus_tag="PLF14_04930"
     CDS             33342..34040
                     /gene="araD"
                     /locus_tag="PLF14_04930"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006808540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase AraD"
                     /protein_id="HOT70672.1"
                     /translation="MNNLAQLKQRVYRANMDLVKHDLIVLTWGNASAIDREKELVVIK
                     PSGIEYDKLQPEDMVIVNMDGKVKEGSLKPSSDILTHIELYKAFPEIGGVVHTHSCFA
                     TAFAQAGKPVLPFGTTHADCFLGPIPCTRFLKDRELADYEKNTGKVIAETFTSLDYRT
                     IPAALVAGHGPFIWGRTPEESVLHAVTLEAVAKMAFLTITLGREQCIGQKLLDKHYFR
                     KHGKNAYYGQEKNK"
     gene            complement(34258..34728)
                     /locus_tag="PLF14_04935"
     CDS             complement(34258..34728)
                     /locus_tag="PLF14_04935"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOT70673.1"
                     /translation="MITVCCYQSKIGRINISEDNEGICGVSTCTAKDCSHSSQSKLKD
                     ETFRQLAEYLQGKRKSFNVKLSLKGTEFQKSVWEQLLNIPYGETRSYGEIAALIGNAK
                     ASRAVGMACNRNPVMIIVPCHRIIGKKGCLTGYAPGIDLKEKLLDMEMELKSNR"
     gene            complement(34728..35801)
                     /locus_tag="PLF14_04940"
     CDS             complement(34728..35801)
                     /locus_tag="PLF14_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HOT70674.1"
                     /translation="MYNGLGSDLSSLSRLSDAQSRSISAENFKGEKGKGGMATEGTGA
                     YPASDLGGLGWKVSPSIVLQPGELFEIADIEGPGAIKHIWITSGGTGRDTIIRFYWDN
                     CEYPSVECPIGDFFANAFLPIHIPINSLAVCNNSVRGFNCYWEMPFRKHCKITLENVG
                     PKRAYHYYQIDYILTEIPDDCAYFFAQFRRENPVKYKTDYTIIDNIKGKGHYVGTYMT
                     WGANNNGWWGEGEIKFFMDGDDKFPTICGTGTEDYFCGAYCFMENDRYLDYSTAYSGF
                     YPVRYEKEAQGQQRFSMYRWHITDPVRFEKDLRVTMQALGWRSNTKYLALQDDVSSVA
                     YWYQTLPFNKFPTLPTYEFLEII"
     gene            complement(35820..36512)
                     /locus_tag="PLF14_04945"
     CDS             complement(35820..36512)
                     /locus_tag="PLF14_04945"
                     /inference="COORDINATES: protein motif:HMM:NF039407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6320 domain-containing protein"
                     /protein_id="HOT70675.1"
                     /translation="MPYCVKCGVELDNSAKKCVLCNTKVMMELPDEIPPYPKEKLEIS
                     QLNRGYFSILLTMILLVPNLACLVINLIYTPGVYWMYYVFGGSLLLWMIFIFPMAIKK
                     KRPLLHIFLLFLSATLYLLLIALALSGMRWFLLIALPICITVMSLLYLIILIVIKKKP
                     GKLKTVSISLIAVIIICLVTDGVIDYYIYSDIQLSWSYIVAASIIPVIIGLVLLAGNK
                     KLQNELIKKLHI"
     gene            complement(36523..37818)
                     /locus_tag="PLF14_04950"
     CDS             complement(36523..37818)
                     /locus_tag="PLF14_04950"
                     /inference="COORDINATES: protein motif:HMM:NF018902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol acetyltransferase"
                     /protein_id="HOT70676.1"
                     /translation="MLRKIEEKKYRHWFKLDNAAKIYPAANTRRWSGMFRLSVTLNEK
                     IDPVILQEALVATIKRFPSFNIRMGKGIFWYYFESNIRKRLQVKPDINNPCAPIRWKD
                     NNGFLLRVFYYDKRIAIDFFHSLTDGTGAMVFLKTLTARYLTLKGYEIPTGEGILDVE
                     EKPDKEEYEDSFVKYANSRQRGRPKEPKAYHMKGTGLDDLDISIITGIVSLEKIKQLA
                     KEHHATITEYLAAILLYSFYKQKEKEGLYKKPTRIAVSIPVNLRNYFKSNTKRNFSTF
                     LNPGIDTILGEYTMEEMLRQIQYFMKANLNDKVLNAMMTQNVILERNLFIRIVPLFIK
                     NFIMSLAHDTIGYRPITTVISNIGIVTVPPEMKELVNRFEFILGPAKIRTPYLAVVSY
                     EDTLVLNFTSKIQETDIERTFFTYLIKQGIPVKIESNRE"
     gene            complement(37803..38285)
                     /locus_tag="PLF14_04955"
     CDS             complement(37803..38285)
                     /locus_tag="PLF14_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOT70677.1"
                     /translation="MNIYDKWNDKLKSDDAAQFYNTYLEQETEAYKAILAEKDKHLKG
                     TVAELAEKFGMEDWVFAGFMDGINTSLKKNVNLDTLNNDTALNCEIDFEKLYYNMHNA
                     KAKWLYSLDEWEDILSKEERERIRKQYVADHTAVRDKVGRNEPCPCGSGKKYKKCCGK
                     "
     gene            complement(38294..40192)
                     /gene="thrS"
                     /locus_tag="PLF14_04960"
     CDS             complement(38294..40192)
                     /gene="thrS"
                     /locus_tag="PLF14_04960"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOT70678.1"
                     /translation="MIKIKLPDGSIKEYENAVTALSVATDISRGLAKAVVAAKVNGKV
                     KGLTEPIENDADLLLLKFEDEDGAHVYHHTSSHIMAHAVTLLFPDVKLAIGPAINNGF
                     YYDFDTEHTFTDEDFALIEKKMEEIIKADYPIERFTLPKDEALKFVKDNPYKYELISE
                     LDENEEISFYKQGDFVDLCAGPHLPSTGRVKVAKIMSVAGAYWRGDEKNKMLQRLYAT
                     SFPSQTMLDEYIYKMEEAKKRDHRKLGRELDLYDIFEEGPGFPFFFPKGMEIRNQLID
                     YWRREHKRAGYGEIITPIILNEELWHKSGHWDHYKDNMYFTTIDEGNYAIKPMNCPGG
                     MLAFKRRMYSYRDLPLRTAELGLVHRHELSGALHGLMRVRCFTQDDAHIFMTEEQMKD
                     EIIGVINLMKKIYALFNFEYKVELSTKPENSMGTKEQWDIAEKALQDALESLNMKYEL
                     NPGDGAFYGPKIDFHIQDSIGRTWQCGTIQLDFQMPERFDLTYMGADGNKHRPIMLHR
                     ALFGSIERFIAILTEHYAGAFPVWLAPVQVKVLPLTDRQADYAQKIRDILLAKDIRVE
                     CDFRSEKIGYKIREAQLEKIPYMIIVGDNEMENNRISVRVRKIGDIGTMFLEEFQNRI
                     VKEIENKL"
     gene            complement(40691..42388)
                     /locus_tag="PLF14_04965"
     CDS             complement(40691..42388)
                     /locus_tag="PLF14_04965"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HOT70679.1"
                     /translation="MKFSQMPYKRVTYQEIEAKAKALTAKLSNAKSAKEALDTFYKYN
                     EYSDKITTAGSLAYIKFTLNTEDEFFAGEKEYYDEIMPMVQNLDLQFKLTLYNSPFKE
                     QLKKELGELLFLNIEIYMKSFDPKIIPHMQEENRLTNEYEKLLASAQIDFDGKVLNLS
                     QLGKYKQSTDRAVRKAAYEAEAGFYLKNSDALDNLYDQLVKIRTHMAREMGYENFVEL
                     GYYRMMRNSYTKEDVARFRGYVKKYIVPIVSELKKEQQRRIKVDKFMLYDDPLEFLSG
                     NATPKGDPEHIFAQGKKMYSELSPQTEEFMNFMLENELFDVISRKGKAVGGYSTTIPD
                     FKAPFIFANFNGTSGDIDVLTHEAGHAFASYLARDFKISEYRSPTMESCEIHSMSMEF
                     FTWPWMHLFFEEQTDKYKYAHLGSALSFLPYGTIVDYFQHIVYEQPDLTPDQRKEVWK
                     KLQKEYMPYLNTDGMPFFEDGRYWQRQHHIYSAPFYYIDYCLAQTVALAYWAESQEDF
                     KKAWDKYYTMTSRGGKDTFTGLVKSAGLPSPFEEGALKKVASVAREWLKSFDKEKLV"
     gene            complement(42385..44286)
                     /locus_tag="PLF14_04970"
     CDS             complement(42385..44286)
                     /locus_tag="PLF14_04970"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HOT70680.1"
                     /translation="MYTRNSRIKTLFNAPLGKDVLDLIAYMSGKGAFLFKNPIVGNIR
                     VGRLPRLTKNQITEDFVDSLIDMLNREPDLHKSPTSELKKTWWKSAVFYQIYPRSFKD
                     SNNDGIGDLNGIVSKLDYLKYIGIDAIWLSPVYDSPNDDNGYDIRDYKKIMTEFGDMA
                     DFDLLLREVHKRGMKLIMDLVINHTSDEHEWFKEALKDKNSKYHDYYIFRKGKNNGPP
                     NNWTSAFKGSAWNYYPHLDEYALHVFSKKQMDLNWENENMRKDIYDMINWWLEKGVDG
                     FRLDVINLISKTPGLPDGLPALKGLVGAIGYEHMFYGPKLHEYLREMRKNTFDKHNAF
                     TVGEGAGIGLEAAKLMTEESRKELDQIFNFDHFNNPGKSRHDSYRYDLRKLKPLLVKW
                     QRDFKDGCWPALVFENHDNPRFVSRITKDESKRDLVAKLIITLMLTLRGTPYFYQGQE
                     IAMIDSVFKEISDFRDVEAINMFNEYLEQGLSEEQAILKISAGTRDHARIPMQWEDAA
                     YGGFSEVKPWIDCSNDYMRYNAKSQIKDTFSVLNYMKSLISFRKENQTLIYGDFILTD
                     NDADTFCYERKDKDARFYIEINLIDSNKKRKIDVRDKKYKKILSNYPDSNEFLRPYEV
                     AIYMIGEEK"
     gene            complement(44444..>44812)
                     /locus_tag="PLF14_04975"
     CDS             complement(44444..>44812)
                     /locus_tag="PLF14_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70681.1"
                     /translation="WYLNGEGFYEYTYETRKTEHVADVAGEGFYSVSYNDEYIYACQY
                     FETEDSVTSPMFYIFDRQYKLIDSVKIDIEEKCYNPLYLTELDDYIILSTSTWNRPPD
                     YYLLKSEIGSGDIKVRKIGE"
BASE COUNT        12656 a         9823 c         8470 g        13863 t
ORIGIN      
        1 ccgcatgcga cacctcctaa gattgccgca cccaatgccg acccttcgga attgaccaca
       61 gtggctatgt cgctttcaaa tatatcggcc tgcatggttt tccacagcgg acttttggct
      121 ccccctcccg aaaccctgac ctggcttaca tcaataccca tttctctcaa tatacagaca
      181 ctgtctttca gcgaatacac cactccctcc agtatcgccc ttataaaatg cgcttttccg
      241 tgagtgggcg taatgccgaa aaacactccg gaacagaaag gatccaggtg aggtgatcgt
      301 tctcccatga gatacggtag gaaaatcaat cctcctgctc ctattgctat ttcctccgcc
      361 attttatcca ttattacata cgggtctatt cctttttttg ctgccgcatc gatttcttct
      421 ttgcagaaat tatctcggaa ccattttaac gacaatcccg cagcctgtgt aacacccata
      481 acatgataac atccgggtac ggcatgacat aaggtgtgaa tccttcctaa cttatcaata
      541 tgcagtttat cggtgtgagc aaataccaca ccgcttgtac ctattgtaga agatactatg
      601 ccttctttta ctattccgtt acctactgca ccgcaggcct gatcccctcc ccctgcggca
      661 actattgttc cttctttaag tgaggtcaaa gaggcagcct tttttgttac tttgcctgct
      721 gcatccgccg attcgtgcat atagcccagt aatgaagtgt ctatttccag tgcatcacat
      781 aattccttgg aaaatactct tttctttaca tccatcagct gcattcccga accgtccgac
      841 acatcggcac tgaactcacc ggtaagcatg tagagaatat agtctttggg cagcagtatt
      901 tttttggctt ttttatataa atccggtcgg ttttgcataa cccataatat tttagctgcc
      961 gtaaatcccg taatggcggg atttgcagtt atgcttatca atctgtctct tcctatttta
     1021 tcttcgaggt attccgcctg tttttccgtt ctctgatcgc accatattat ggagcgtccc
     1081 agcagtcggt ctttttcgtc cagcatgaca agaccgtgca tttgtccgga aatacctacg
     1141 cccttaataa gtcctctgtc aatgccggta tccgcaatga ttttcctgca tgtaaaacac
     1201 acggcgtccc accaatcatc aggatcctgt tccgcccatc caggattagg ctggtagagc
     1261 ggatattcat acgatgcgga tgcgtaagcc tttccgtctt cactgaataa aacggatttt
     1321 gtggctgaag tacctatatc tattcccaat aaataattca taaataacct ccgtttacat
     1381 atttattaat ttccgtataa cataacctgc aatgataagt cccgctgtcg aaggcacata
     1441 agatataccg gatatgagat tggggtcgct tacctgattc ttacttacgg aatacagtac
     1501 atccacaccg gttatgcctt cggaagataa tattttccta acctttcttg ccagaggaca
     1561 ggtatcggtc ttttcaacag gtgcaatttt aaaaccagta ttatcaatct tatttcccgt
     1621 tcccatgcag gatattatgt ttatgccgtt tttcttggca tatactatta tatcaatttt
     1681 tgatgaaacg gtatcaattg catcaattac ataatcacaa tctaacggta ttatatcttt
     1741 tatattgtct ttacttacaa aaacattatg cacggttact ttaatatcgg gatttatgtc
     1801 ttttacacgt tctaaagcaa cctccgtctt atactttccc acggtggaat gtaaggccag
     1861 tatctgtctg tttatgttgc tttcttcaat cttttcataa tcggcaagta tcaagtgtcc
     1921 gatacccgct cttgcaagag cttccagaac ataggaaccc actcctccga gccctattat
     1981 tgcgactttc ttttgacgca gctccttcat tgcctgctct cctataaggc ggattgttct
     2041 gtcataaata gcatcactca tgtattcacc aatactttca ttgcattttt gaagtaatca
     2101 tcgggcggtg cggtaaactc catatattct ttctttaccg gatgtataaa gcccagaaga
     2161 taagcatgca gaagttgtcc cttgttgaac cgtttgtcct tacctccgta aatgtcatca
     2221 cccaatagag gattccctat aaaggccata tgcaccctta tctgatgggt tctgcccgtt
     2281 tccagcatcg cttttatttt tgatgcaccg tgcatcttct ccaatacttc aaaatgagta
     2341 actgccttct tgccgtcaga atccactgcc attttcagcc tgttaagcct gtctcttgct
     2401 ataggagcgt taataaggcc tttgtcggaa ttcggataac ctttaactat tgcaatatac
     2461 tcccttttta catcatgtcg tgcaaatgat tcggttaaaa ctctatgcga atagttattc
     2521 ttgcaagcca gaataacgcc ggaagtgtct ttatcaatcc tgtgcactat tcccggacgt
     2581 tccaccctgt ttatatcact caaactgttt tttgtatggt acagcagggc gtttacaagg
     2641 gtatcggaat agtttccggt agcgggatgc accaccattc cccttttttt atttatcact
     2701 attacatcgt catcttcata taatatatcc agaggaatat tctgcggttg tatggaagaa
     2761 ggcaaaatgt cgggcattat tactttaata acatcattat ttttaacaga ataacttgca
     2821 tttcttacca gactattaac ggtaacctgt ccttctttga taatcttctg tataaatgac
     2881 cgggaataat cttctatgta tttggtaaga taaacatcca gtctcattcc cctatccgaa
     2941 tctgctgaaa atacaagttc attcaccgaa tcattatcct gcaatattat ctcatcacaa
     3001 atatccttta tattatccat actcacggtg acatctccct gtgaataaaa agcatgtaga
     3061 ttataagaag aaaaacccct accgttatag cggaatcggc aacattgaat gcgggaaaat
     3121 catacccgaa aatatacgca tctatgaaat ccacaaccca gcccaacctt atacggtcta
     3181 tggcatttcc caccgctcct ccgattatga gagccaggga aaaggctccg aatgctcttt
     3241 ttatgtataa aagcaggaca ataagggcaa ttgtggctac aacggaaaat attgccaagt
     3301 attcggtatg ttcgccgaat attccccatg ccgctccggt atttctggtg tgggtgatat
     3361 ataaaaagcc gtctataatc tttatgctgt cgcccaactg catgttgctg acaataaaga
     3421 acttggacag ctgatctgcc gctacaatca taatactgag aaacaaccat agaatattct
     3481 taaaattaat ccttctcaac attgtttatt ctctcaatgt ttattgcttt accggtcttt
     3541 tcacataatt ccagtataac cgcattgagt tgcccccttc cctcctgact cgtaaagtat
     3601 gccggcattc ccgtaagaaa tttctctatt accgtatccc ttgaagcccc tattatgcct
     3661 tcatgcggcc ccgtcattcc cgcatcggtt ataaaagcgg ttccgaaagg caatattctt
     3721 tcatcggcag tctgcacatg agtatgtgtt ccgataaccg ccgatactct gccgtcaaaa
     3781 taccatgcaa atgccgcctt ttcactggtg gcttctccgt ggaaatccac cactatattt
     3841 ctcgcttctt tcttaacttg gtccagcact gactctacgg cataaaacgg attgtcgcaa
     3901 ggctccataa atgctcttcc cgatacgtta ataacgcaaa gaggagaaca accgttttta
     3961 agtatagtat aaccttttcc cggacaagtc tttgcatagt ttaacggtct taaaagctta
     4021 atattttcat ccgaaaaagc aggtaatatt tcttttcttg cataaatgtg atttcccgat
     4081 gtaacaacat ccgcacccat ggataataat tcctgtaaaa gtgtcggtgt tatgccttta
     4141 ccccctgcgg tattttcacc gttggcaata acataatcgg gagcatattg ctcttttaag
     4201 taagcaatgt tgttctttaa gacaactctt cctgtcttcc cgtatatatc gccgataaat
     4261 aagactctca ttcaaacctc gattctattg ttttattata atccattcac acacttttga
     4321 gaagtaacca taacaaccgg ttgcagcact taacattttg ctatactatt tacctataca
     4381 acaaagagga cataatgaaa actcataaaa aaggcatttt aaacagaata aagcaacccg
     4441 atatacaaaa aagtatacct tttcatatgc cggggcataa gagaaatgta aacttcaata
     4501 aacacctgaa taaaatcggc atcaggtatg attttaccga aataccgcag ctggataatc
     4561 tgcaagctcc ggaaggcatc attaaaaatg tcatggataa agcacaactg ctttggaata
     4621 ccaaaaaaag ttttctttcg gtaaacggaa gcacctgcgg aataaatgcc tctatagcca
     4681 cactggcaga cctgaaagac aagataatag ttgcaagaaa cagtcataag tcggtatata
     4741 atgctattga gcttctggac ctggaaccgg tctttatagc ccccgcattt aacaataaac
     4801 tgaacatatc cggctcaatc aatcctgaaa cggtctataa tgcgatattt tgcaatcccg
     4861 acgccaagac ggtgctctta acaagtccca cctatgacgg cgttataagc gatataaagg
     4921 ccatttgcga tatatcgcat aagatgaata taccggttat cgtagatgag gctcacggag
     4981 cacacttgca tcttagcaac tgcttcggca acagtgcaat ttcagcaaac gccgatattg
     5041 taatccaaag cctgcataaa acacttccca gcctgacaca gacggcgata gcacacatat
     5101 gtacggacag aatagatgtt aataagtttc aaagatatct ttcggttttt cagacaagta
     5161 gtcccggtta tccgttactt tcctcaattg aaagctgtat tgacctttta ttaaacaata
     5221 aagacaaact atactctgac tggataaaga acattgacta tatagataac agaatatccg
     5281 aacttaacaa cataaaagca ttttatcacg gtttatccaa aaacgaggta tacgagaata
     5341 tatacgcata tgaccgatcc aaacttttga tatcgacaga taaccttaac ataaacggca
     5401 aacaactgtt cgacatactg atgcaagatt acggtatcat atgtgagatg tatacaaact
     5461 cctatgtttt ggccatgctg tccatgtgtg acaagaaatc ctccgtaaga aaactgtgca
     5521 atgctttgga agaaatagat ttaacttacc ccaaaaataa taaaaatacc ataaaaaaca
     5581 ctcaaacata tgatattccc aaacggtgta tgagtataag agaggcaaga aacaaaaaaa
     5641 gtatgagtat ccccctttcc gaagcacagg gacatatctg tgcggaatac atatgggcat
     5701 atcctcccgg tatcccctac ctcattccgg gagaaaagat aacacgggaa ataacagata
     5761 ttcttaaaga agcaattgac aacggtatag atttaaaaac cgataataac gacaacgaag
     5821 gctgtatcac ggtattgtgt aattgacata ttaccttatt taatctaaaa ttatcctgta
     5881 aaatatgatt ttgtaaaagg accggtaaag gaaatggaaa agacaaaaag aattaaaaca
     5941 atagagaccc gtaatttggt aagcagtatt aaaaacggcg gttgcggcga atgccagaca
     6001 tcctgtcagt cggcttgcaa gacttcctgc ggagttgcta accagaagtg tgagaatact
     6061 gataagtaat tgctcttaat tgtttatgcg ccgtcctatc tgcaaactac gggtatgacg
     6121 gctgtttttt tgtaaaaaag agaaaatgat acatcaatac aaacttaacg gttacaatat
     6181 agtgcttgac gtatacagta attcattgca tgtaacggat aatctcgcat atgacattat
     6241 cgctctgtac aaagacaaat ctaaagaaga gattgttgaa attataaaaa acaaatatcc
     6301 cgatctgaca aataacgata tatgtgagtg tctttcggat attgagcagc tggagcggga
     6361 taacaagctg ttcagcgagg atgtatataa ggatgttccg gttaataaaa tatctgaagg
     6421 taacatagtt aaagcattgt gccttcatgt ttcacatgca tgcaatttaa attgcagtta
     6481 ttgctttgcc agtcagggca attacaaagg taagcgtgct ttaatgagtt ttgaagtggg
     6541 caaacaggct ttggactttc ttataaaaaa ctcaggcaat gtaaggaatc tggaagtgga
     6601 tttcttcggc ggagaacctt taatgaacct ggatgtatgt aaacaacttg tacagtatgc
     6661 aagaagtatt gaaaagcaac ataacaagaa cttccgcttt acccttacca caaacggtat
     6721 gctaatagat gatgatgtca tttcgtttgc caaccaagaa atggataatg tggtactcag
     6781 tcttgacgga agaaagcatg tacatgacag attgcgtaaa gatattaacg gaaacggcag
     6841 ttatgatgtt atagtgccca agtttaaaaa gctggtggat aaccgcacaa aaggaaccta
     6901 ctacatacgg ggaacattca cacatcacaa caccgatttc acaaacgaca tattacatat
     6961 ggccgatttg ggatttaaag aactgagtat ggaaccggta gtatgctctc ccgatgatga
     7021 atatgcactt aatgagaagg atttacccgt tcttcttgaa cagtatgagt tactggcaaa
     7081 aataatgttg gagcgggaaa aggccggaaa aggattcacc ttctaccact acatgattga
     7141 cttaaatcac ggaccctgta tacacaaaag attatccgga tgcggttccg gcagagaata
     7201 tcttgcagta acaccgtggg gagagttgtt cccctgtcat cagttcgtat ccgactcccg
     7261 atacagcatg ggcgatgtat ggaaaggcgt gacaaatacc ggtttgcgaa agaagtttga
     7321 gctttgcaat acatacacaa aagaagaatg taaaaattgc tgggcaaaac tgtactgctc
     7381 cggaggttgt gccgcaaatg catatcatgc aacaggtaac atcaacggta cttacgaata
     7441 cggatgtatt cttttcaaga aacgcatgga atgcgctatc atgctgcaga tagcaagagc
     7501 cgaaacagcg gattaaaagc gttaaagtat aatccgtaaa accgatatta caaatgaaat
     7561 gcacccctaa atggtaaaac tttttatcac caaccgataa ggtaacagat aaacaaaaaa
     7621 tctgaaaaaa aaagcatgta ccgcggcaca tgcttttata cttacttggc ataatctaca
     7681 actcttactt ccctgatgac attgacttta atctgtccgg gatattccat ttcattttca
     7741 attctcttaa cgatatctct gcatatgagc accatatcgt catcatccat cttctcgggt
     7801 tttatcataa ttcttatctc tcttcctgcc tgaacggcga aagatttttc tacgccctcg
     7861 aatgaattag caatctcttc aagtttttca attctcttta cataagcttc caatgtctct
     7921 cttcttgctc ccggtcttgc tgccgatata gcgtcggctg cctgaaccag cacggcaatg
     7981 gcactttccg gttctttatc accgtgatgt gacattattg catccaatac gacaggatgt
     8041 tcgttatact tcctggctat atccgctccg acatcaatgt gagaaccttc tgtttcgaaa
     8101 tctatggatt tacctatgtc atgtaaaagg cccgctcttt tcgccagcgg tacatccaga
     8161 cccagttccg cggccattat acctgtcagc aaagatactt ccagtgaatg cttaagtaca
     8221 ttctgaccgt aactggttct gtacttcatc cttcccaata accttattat ctccggatga
     8281 aggttgtgaa ctccggcttc aaaagaaact ttatcgcctt ctatcttgat ggtattctct
     8341 atctcctttt tagctttatt tacggtatct tctatcctgg caggatgaat tcgaccgtct
     8401 aaaataagct tttccaaagc aattctggca acttcccttc ttatcggatc aaatcccgaa
     8461 agaacaactg cctccggagt atcatcaatt atcaaatcaa ttcccgtaag agtttccaaa
     8521 gcccttatat ttcttccctc tctgcctatg atacgtcctt tcatctcatc attgggtaat
     8581 tccacaacag acaccgtatt ctctgcgaca tagtctgcgg cacacctctg aattgctgaa
     8641 gaaataatat ttttggcaag attttccgat tcttcctttg tctttgcctc atattctttc
     8701 aacagaagga ctttctcatg cttgatatca tcttcgagtg ttttaagaag atatgtcttg
     8761 gcttcttcaa ccgtaagtgc ggatattctt tccagttcgg taagtttatc ctgatggatt
     8821 tgctcaattt ccttttgttt ggaatcaata tccgacattt ttttattaag aatttcttct
     8881 ttactttcca cagaagcgac tttcttgtcc agggcctctt ctctctggat tactcggctt
     8941 tccattctct ggatttcctt tcgtctttct ttgagctccc tttccatttc cactctgctc
     9001 ttgtggactt cttctttggc ttcaatcaat atctctctct tgcgttcttc cgcatttttc
     9061 tcaccctgta agataagttt tttagcttcg gattccgcgc tgcctatttc cttttcagca
     9121 actttctttc tgtgtcgtat ccccaaaaaa aacattacta ttgaaaatat tataagaaaa
     9181 gcgataatga cgagacctat aatttctctg agactcattc ccttttatac ctccttattc
     9241 ggttttcaaa gttcatatgt taaattaaaa taaattatcc atacaattta tgattttatt
     9301 atttttttac atatatgtca ataaaaaata tgtttaaggc gaattattga gatattgata
     9361 atgatatttc cattaattac aagtgtttgt atgtttaatc ttaaccgata atttacctaa
     9421 tactttttgc caaccgttaa tttgtcgttg cattatttac aataagttga tagaataact
     9481 cgtgatgaat tttattggag gattaacatg aagtcagccg gagtagtaag aaaaatagat
     9541 gaattgggaa gaatagttgt tcctatagaa ttacgaaaat cccttaatat aggtaagaga
     9601 gaggcagtgg aaatatccat ggaagatgat tgtatactta taaggaaatt tgaaccggga
     9661 tgctgcttct gcggttctac cgatgatctt gaggtatatc acggcaataa agtatgcaac
     9721 aaatgtatta aagatttgtc atctgctacc gaataatatc gcataaacgt catttcttga
     9781 catgttaaac aaaacggcaa cttttcgcat ggcatctttg cgggaaaagc cgttttttat
     9841 aagttcctca tatttctcgg ctatatcccg ttgggaatga gggcaagggt tttccgtttc
     9901 attgtttgta tcggtattac cctcgaaaac cagtacatac tcccctttcg gcgggttctg
     9961 ctccagatat tccgctatac cgtccagact gtccacaaca accgtctcat atatctttgt
    10021 tatttctttt accagcgata ctcttctgtt tccgcccata acttgtcttg cgacatctat
    10081 tgttttaata agtttatgag gtgcttcgta tactatgcac gtacgtgatt cattgcgaat
    10141 cttctcaaag aattgtctct tctccgatat cttcacgggc ggaaagcctt caaaacaaaa
    10201 agaagaagtg tcaaagggcg atatcatcag ggcacttatt gcggcattgg gtccaggtat
    10261 gaccgttacc ttctggttgt gcagatgcat aagttttaca agctcgttac ccggatcgga
    10321 tattgcggga caaccggcat cggaaactaa acagacatcc attccgttca taacatcttt
    10381 aagtatactc tgtccgcttg actccatatt gtgctgatga taacttacaa gtttcttctt
    10441 aatgcctaaa gcattaagca gacgcagagt gtgtcttgtg tcttcagatg cgatatagtc
    10501 gcagttagtc agagtttcta ttgccctttt tgatatgtcg gaaaggttac ctataggtgt
    10561 agcaacaaga taaagcaccg ctaattcctc ccgtacagta tattaagttc ttcggtataa
    10621 ctgccgtccg gtttatgtat tataaacgga ggctccacgg ttattgaggg attcatgtct
    10681 tttaacccct gtatcagcac cagaatagca ggtgatttca cattggcatg cacaaatctt
    10741 attgttttag gttcaaccga ataagttttc atcagtaaca atatctcgtc cagccgctga
    10801 ggtctgtgaa ccattgtaaa aatacctttg gtcttcagaa gatacttggt agcctttaat
    10861 atgtcctcta aggtacacag tatctcgtgc cgtgtatatg ccattgtgtc ggccatgctt
    10921 attatacccg ttcccgtctt cagatacgga ggatttgtta cgacatgatc aaaactgccg
    10981 gttttgaaat actcctttat attccgtata tcgccgttta taacggttat cttatcctca
    11041 agattgttca gttgaatatt tcttacggca agatcatatg catccttctg tatttctata
    11101 ccggttatat gctccggttt atacttaccg tacagaagaa gaggtattac tccgtttccc
    11161 gtacccaaat caataacact cgatgtgctt cttacgttgg caaatgaaga caataaaaca
    11221 gcatcggttc caaagcagaa ctgttttttg ttctgtataa gttttaaacc ctccagctga
    11281 agatcatcca gcctttcatc ttcatgtaac atatgtatac attatacatt tcggaaagtg
    11341 ctttatacaa ataaaacttt cattttagtg gatatttgat gattttttat gatatttaat
    11401 aatatcttga tatgtatcaa ggaaaagccg ttattgaacc ttcagaaaca gtttgtttct
    11461 gtttatggag agttcttcaa cttgtattcc ggtgtacttt tccaaggctt taagcagaag
    11521 tctgggatgc agattgcctt ttgcgccggc tttgcataac acggtaaatc tgttttcatc
    11581 gtaataatct atcttataca gatagggcaa tatattcaca agaccttttt tattggattt
    11641 ctccacttca atactgtcca aagaaataag tttatccagt gcttttttca gatttccgtt
    11701 gcagttgact ctgaatccgt aaatggccga tcccacatcc tccattaaag tggatttttc
    11761 tatcgtgtag gcttccaaca actcaaaccc gtcaggcagt gcctgagaaa acttattcaa
    11821 agcataccgg cattcgtatt cggtattaaa ccatatatct acatactcac tgtcggacga
    11881 cacacctacc ggcagcggta atgcaaaaac cattctgatg ctcgggttga atcctcttgt
    11941 gtaatataca tccaattccg ctcttctgag ggcacgcgaa aaagcattga tcatatcaac
    12001 gtgtgctaca tatttgacct tttcggtttt tctgaattta aatcttatta tcttcaaaac
    12061 atactccgca tctgtatttt gctatcccgc agttattaca atgctccctg caattttcgg
    12121 ttgtttcttc cctcagcgcc ttttgatatt cgttcttaag aaaactcttt tttacgccta
    12181 tgtcaataaa atcccacggc aactcctcat ctaacgcaat ctgtttggat gcacactcgt
    12241 aaatgtccaa agaacattca ctaaatgcct gttcccattt ttccatatcg aataactcat
    12301 tccaggaatc gaatttacat cctttttccc atgcttttat aatagcctgt cctacttgcc
    12361 tgtctcctct ggatatagcc gattccacaa ccgaagtgtt gaaatcatgc cagttgtact
    12421 ttattttctt ctcctttata tgacttctga gcagtctctg ccttcttatc atttcctcca
    12481 cggtaatctg agggcaccac tgaaaaggtg tgaagggttt cggtacgaag aaggaagtgg
    12541 ataccgttat atttattctg ctttttcttc cgaatatatc taaaattttc ccggctaatt
    12601 cggcaattgc tatgacatcc tcgtcagttt cggtaggtaa tcccaacata aaatacaact
    12661 ttatgttgtc ataaccgccg gattttgcta tttttgcgga agtcaatatc tcctcttcgg
    12721 taatattctt gtttataaca tccctcagcc tctgacttcc cgcttccggt gcaaaagtca
    12781 gccccgattt gcggaccttc tgtattctat tcataagttc tatggaaaaa ttatctattc
    12841 tcatggaggg aacggataaa ttaaccattc ttttctcggt gtgctccagc attccgtcac
    12901 ataaagtctt caggtcggta tagtccgatg tgctcagcga aaccatccct atttcattgt
    12961 agccggtgtc ggctaaaatc ctctttgtct gctcaatcag cgtagaggct ttcttttctc
    13021 ttaccggtcg gtatatataa ccggcctggc agaacctgca acctcttatg catcctctga
    13081 acaattctaa agaagctctg tcgtgaacaa tattcatata aggaactatg gagtcggtag
    13141 gaaaagaaac agagtccaga ttctttatta ttctttttat caccttttta tcgaaatcat
    13201 gatatttcgg tacataaact ccttcgattt tcacggattc ttttaaaaag tcctcttttg
    13261 attgccctgc acttttcccg tcctcataaa ggtctaaaac ctcattcata acctcttcac
    13321 cctcaccgat aagaaagata tctatgacat cctccaaagt gacgggattt gccgcacaag
    13381 gccctcccgc cataattatc gggtgctcat ctgttcttat acttcgtctt atcggtatat
    13441 cggccagatc cagcatgttc agtacattgg tatagctcat ctcatactgt aaggtaaaac
    13501 cgactatatc aaactctttc aaaggctttt tggtttccag agagaataac ggtactttat
    13561 ttgcacgcat aagttgctcc atatcggtcc acggagcaaa taccctttcg catacggttg
    13621 ttttccttcg gtttataaca tggtacagga tacgcattcc cagatgactc attcccactt
    13681 catatatatc gggaaagcag aatgcatagc ttgattgcat tttgtcatgg tcttttacta
    13741 tctcattaaa ctccccgcct gtatacctgg cgggtttttc tacatttatg agatcaatca
    13801 taaaccgtta ttcctcttct tcatcaaagt ccgaatccat cttttccata aaaagttcga
    13861 aaagttcatc cagcaactgc tcgtcttcaa ccggtgtcag tattgcatca tccttgtcct
    13921 cttcatattc aaatatgtat gcatcctctt ccgcttcatc ggccggctgc atcaaaacat
    13981 aggttctatc atcataatcc agcatctcca gaaccatcat ctctatagga ttgccgtcat
    14041 catcttccat cacaattatg ttgtcccttt cgtcatccat tgccttttct cctttaaact
    14101 ttttatatgc taaatatata tatctattat ttcttttttt tattcatttc atcaagatag
    14161 gattgcagta tcacacaggc ggctaccata tctactcttt ttttgtcttt tcccgtcttc
    14221 tttccctgca tgtgcattat gtcatgcgct tctacggtgg aaagtctttc gtcaaacaat
    14281 attaccggta atttgcaccg ctcttttagc atggatgcaa aggtacggac ttgcatcgct
    14341 ttttcaccct ccgagtcatc catattgaga ggaagaccta caactatacc gcttgctttg
    14401 tactcgtcaa taatcccctc caatatatcc agaggtctat ttatgtcact attccattct
    14461 attgttgtaa caccgtgagc cagcaaaccc aaagggtcac tcttggcaac ccctattctt
    14521 ttgtcaccgt aatcaattgc aataattacc atctaagtta ttttgatgcg aaataatact
    14581 gaataagctc agtcattatt tcatcccttt ccaactttct aatagcattc ctggcgccgt
    14641 tatggctggt tatataggtt ggatcccctg acataagata tgcgaccagt tggttaacgg
    14701 gatcataccc tttttcggtc atggcactga aaacaagtgt aagtatttct cttgtttcgg
    14761 catgatgacc tttcagaaag tcaagattaa ttgttgatcc tctatccact ttacatatct
    14821 cctttgcagt tcatatttgt agtttactgt aaaacaatgt atttttcaag gataaatatg
    14881 taaattcaat ctatagtttt ttaaattgac cgctttatcg gacagttttt ttatcaacaa
    14941 ccgtacccag aagtccgtca ttcgtcaggc cggaaatcaa tacatcgcaa aatgtattga
    15001 tatagtcgtc cgttacattg ttatccgtcg gagcctgcac ttttatatag tttgccgtat
    15061 gaccggtaat gaaaccgtct tcgccctctt caataagtat ccgtaatgtt tttccgagat
    15121 accctgaaag tcttgatttt gatatttccg atgcataatt tattaacttt tcgcttcttt
    15181 cattaagagt tttaccgtta agaggcttga gtttatccgc tgcggtaccg ggtcgtttgg
    15241 agtatttaaa gacatgcact ttgataaacc gggctttttc caccatttcc agacttttat
    15301 taaagtcctc ttccgtttcg gtcggatatc ctactattat gtcggtggta aactccagat
    15361 tgggaatgtt gtttctcaac atgtttattg cacttaaaaa catttccgaa tcataccgtc
    15421 ttctcatatc ggataacacc gcatccgaac ccgattgcaa tgacatatga aaatgtggac
    15481 ataatttgtc gtatttcttt atttcttcca caaaagtttc cgttattatc ttgggctcca
    15541 gagagcccag ccttatcctt tcaatacctt ttatgtcatt tatctttgca ataacatcaa
    15601 taatactcag attacctttg tctttgccgt aagagtccaa atgtatgcct gtaagtacaa
    15661 cttctttaat gccctttgat gccatatatc gggcttcttt aacgatatcg tcaatatccc
    15721 tgcttctggc tcttcccctt acataaggta ttatgcagta agaacagaaa ttatcgcatc
    15781 cgtcctgtat cttaagatac cctctggttc gctcccattt atcgggaaag caaccgtgta
    15841 tatattcttt ttcacggaaa atatccgaaa caaccgttaa tttaccggtt ttttccttta
    15901 aatatgatga aagtatcgta tcaatgctgc tcttttcgtt atttcccagt actatatccg
    15961 ctccgctttt tatcatagcc tgtgcattgg cttgagccga gcatccggta gcgataataa
    16021 tgctgtcagg atttgtcgtt cgcgctttac ttatcatctg tcgtgatttt ctgtcgctca
    16081 tagcggtcac ggtacatgta tttataacat agatgtcgca aatatcggga aactttttgt
    16141 attgaaaacc cattgcatca agacttgatg caatggtcgc cgattcatat tgatttactt
    16201 tgcatcccaa ggttgctatt gaatatgtct tcatcatttt ttcagttgat gtccaaatcg
    16261 ggattattca tctgcatttc cgcccactcg tctctggaga taagtaccgc tctgggtttg
    16321 gaaccttcaa aaggtcccac tatgcctctt aactccatct ggtccacaat tcttgcggct
    16381 ctgccgtacc ccagtttcag ttttctctgc agataggaaa ccgatacctg acctgcttcc
    16441 accacaaggt ctattgccgc aggcagcagt tcgtctactt cattttccaa gtcgccgtcc
    16501 gcaccttttt tgtccgcttt ttctatattt tccactacat cttcatcata agttgcagag
    16561 gttctttgtt taataaaatc caccaccttg tttatctcgg catcggatat atatgcaccc
    16621 tgcattctga ccggtttgga caagcccatg ggatggaaaa gcatatctcc gtttcctaaa
    16681 agtttctccg ctccgcccat atccaatatg gttctggagt ctacctgact taccaccgca
    16741 aaggaaagcc tggacggaat gtttgctttg ataacaccgg ttatgacgtt gaccgaaggc
    16801 cgctgtgttg cgattataag atggatgccg gcagctctgg caagttgtgc cagtctgcat
    16861 atggattctt ccacttcgcc cggattggac atcataaggt cggccagctc atcaattatt
    16921 atcacgatat tgggcagttt tttcagttgc ggatcgcctt ttaccgagtc gttgtatgta
    16981 ttgatatccc gcactcccct tttattgaac atgtcatatc tgttggtcat ttcctctacc
    17041 gcccaattca aagctcccgc ggctttttta agattggtga ttaccggaat caacagatgc
    17101 ggtatcccgt tataattaat caattccact ttcttggggt ctatcattat aaagcgcaca
    17161 tcatcgggag tggctttgta taaaatactg gtaataatac agttgataca tacgcttttt
    17221 cccgaaccgg ttgcaccggc tataagcata tgaggcatgc gtctgacgtc ggcaaccgtg
    17281 cagttaccgg caatatcttt acctattgct atggtaagaa tgctcttgga actctggaat
    17341 tccttggaag caatgacttc tttaagtgag accacactct ttttggtgtt aggcacttct
    17401 atacccacta cggactttcc cggcaccgga gcttctatcc tcacatcccg tgaagcaagg
    17461 tttaaggcta tatcatcggc cagagccact atcctgctga cttttacacc tgctttgggt
    17521 tgcagctcat atcttgttac ggtgggaccg cacatgatat tggttaccgt tacttccaca
    17581 ccgaaactca gcagcgtatt ttccagcttt cttgctatct gttcatgatt atcggtatta
    17641 ccgctgtcca cataggaatc cagaagcgaa agaggaggaa atacgtactc acccttaatc
    17701 tggggttttt ccgccgttac cgaagcttct tttaccggct cctctttctt tataattatc
    17761 ggaccggtat atttaggtac cgtactcttg gtttctttta ccggtttgga tttgtttata
    17821 attatatttg caggctctct gcttattgtt tctttaacag gttcggcaat atcattaatc
    17881 gtgccttgta catactgctg ttttttcgat ttgacaggtt caggctcttc ttcctgctcc
    17941 tcaatgggtt tgaatgcatc tttcatccag tggaaaaact tgacaaataa gttttttatc
    18001 tttaccagaa aatccagaaa acttttgttt gtaatgtata taagcagagt gagtgaagcg
    18061 gttacggcaa ctatatatgc acccgttact ccgaaaaagg ctactatagg tgtcataatt
    18121 ataccgccga acacaccgcc gttatagacc tgtgaaatac ctatcgaata catatgtgct
    18181 atgaacttcc agacattcat gtcaacggct atatccgcca tatttttgga taaataggct
    18241 atctggaaaa agcacggtac catgaggatg agaattataa ggtacatggt tttcctgctt
    18301 aaccacttta ccctccccgc aagcgccaca tacactccga agagcaatat gactataggt
    18361 atgaggtaat gagcccaacc gaataagcct gcggtcacct gtttaaacgg tcttccgaat
    18421 gcaccggaat tactggtaaa gaatatgcag atgaatgcta aaagagctaa agcgattatt
    18481 acaatgccgg ttatttcttt tgccagtaca ttatttttag ttgaagtttc gggtttttca
    18541 gttttctttt gagtaacaga tgttcttttc ttttgtgcca tgtaatcctc cgattgttaa
    18601 agacatttta tcacaataga ttttgctatg caaacatgat aaaattgccg gaattgacac
    18661 cgataaaacg atgtgataga ctcataggaa attttgaggt gtaaaaaaat gagcgttaca
    18721 tatataccga aaggatatcg ttcgaaatta aatctgtatg aaacacagct ggcaataggc
    18781 acattaaaaa gaatattcga ggagcacctg tgcaaagcga taaatttgaa aagagtctcg
    18841 gctcccctgt ttgtcagagc caattcggga cttaacgata atcttaacgg tatagagcga
    18901 ccggttgcat ttgaaaccaa agaaggcggt atgcaggcgg aaatagtgca ctcactggct
    18961 aaatggaagc gacttgcact gcataaatac ggattccccg caggagaagg cctttatacc
    19021 gatatgaatg cgataagacg tgacgaacag atggataaca tccattcaat atatgtcgat
    19081 cagtgggact gggagcgggt aataacaaaa gaggaaagaa cggtcgcttt tctgaaacag
    19141 atagtaagcg acatagtaag cgccatatgc gacacttacg aaagaatgcg cgatatattc
    19201 cccgctttgg acacggatat ttcgcgtgaa gtgtttttta taacggctca gcaactggaa
    19261 gacatgtatc ccgacctcac atcaaaagaa agagaagatg caatagtcag acagcacaag
    19321 accgtgttta taatgcagat aggtaaaaca ctgaaatcgg gacagaaaca tgacggcaga
    19381 gcacccgact atgatgactg ggaacttaac ggcgatattc ttatgtacaa tgaagtactg
    19441 gattccgctc tggagatatc ttccatgggt ataagggtgg acagcgagtc tctggctcga
    19501 cagcttaagt tgtccggttg cgaggacaga aaagagctta tgttccataa aatgttgctg
    19561 aataatgaac tgcccttatc catcggcggc ggtataggac aatcccgctt atgtatgctg
    19621 cttcttaaaa aggcacatat aggcgaagta caggtctcaa tatgggatag agatactata
    19681 gaggcatgta aagaggctaa agttattctg ctttaaacta cacactgtat aaaatatcgg
    19741 aatatgcagg cataggccaa taactctttg caaccacaca ctccagactg tctgcgaaat
    19801 gtctcaactc tttcattgcc ggcacaacat gatctttaaa agcatatgcg tctaccgatt
    19861 gctccgtaac cttttccagt ttagatatac attttgaaag accggtaatg ttatcggaga
    19921 tatatgttgc aagttctctt tccgttgatg cgtccagacc tatatcggtc ttcattgata
    19981 cggaaccgta tacggttctt gcatagtcca ttgcggcagg cagtatttcc cgctttgcta
    20041 tttccagcat ggttctggct tcaatggata ttatcttaca gtaattctcc atctttattt
    20101 catggcgcga caccagatcg gcttcggaaa aaataccgaa tctttcaaat agctgtacat
    20161 tctttttgtc tacaagacag tccacggctt ccggtgtgga attgagaacg ggcagtcccc
    20221 tcttctttgc ctcttccacc cattcttcgg aatagttatt tccgttgaat attattcttc
    20281 cgtgtttatt gacggtctct ttgataagct tgtctacttc cttgtcaaaa tcttccgctt
    20341 tttccaatat atcggcaaaa tgtttaaaag aatcggcaac aatggtgttt ataacggtgt
    20401 tggcatcgga aaccgaacag gaggagcccg gcatacggaa ctcgaacttg ttcccggtaa
    20461 atgcaaaagg agaggtcctg ttacggtcac atgtatcttt gaaaagtacc ggtaacgttt
    20521 cgcttatatg aaacttttct cttgtcctgt tatgtacctt tctatcattg gctatgtctt
    20581 ccagtatctc ggtcagttcc gctcccaggt ataccgatat gatgcccgga ggtgcttcgt
    20641 gcgaccctaa tctgtggtca tttcccgcgg tagcgatact ttgcctcaac agtccggcat
    20701 attggtccac ggctctgaga acagccacta agattaataa gaatctcttg tttcgggcag
    20761 gatttttacc cggttttaac aacatctctc cctcattggt ggcaatggac cagttattgt
    20821 gttttcccga tccgtttata ttctcaaacg gtttttcatg caaaagacag gcaaggccgt
    20881 gatgttccgc cactttcttg agcatttcca tgataagctg gttatggtct gccgctatat
    20941 taaccgttgt atagataggg gcaatttcat gctgagcggg agcggcttca ttgtgctcgg
    21001 tctttgcggt aatgcccagt ttccacaact ccacatccag ttccctcatg aaattctgca
    21061 ctctctcacg gatattaccg aaatagtgat catttaattc ctggccctta ggtgattttg
    21121 caccaaacaa ggtacgaccg catatgagta agtcttttcg cttatcgtac tgttttttat
    21181 ctatcaggaa atattcctgc tcacatccgg caaaaggcgt tactctggaa gcggtttcat
    21241 ttccgaacaa tcttactatt ctcaaagcct gtttgttaag agcatccatg gaacgtaaca
    21301 gaggtgtctt gttgtccaga ggctctcctc cgtaagaaca gaatgcggtg gggatatata
    21361 gagttttgtc ttttacaaag gcataggacg ttgcatccca agtggtatat cctcttgctt
    21421 caaaagtcgc tcttaaaccg cctgaaggaa aactggaagc gtcgggttcg cctttaatca
    21481 attccttgcc cctaagctcc agtacgggaa cattattgcg tatcataagg aaggaatcgt
    21541 gcttttccgc agtcataccg gtcataggct ggaaccaatg ggtatagtga gtagcacctt
    21601 tgcttatagc ccagtttttc atgctttccg ctacaatatc cgctatactg tcgtccagag
    21661 gacctccctg ctctattgtt ttctttaaag attggtagac ataagcggga agttcattct
    21721 tcatcacctg gtcgttatat acattacttg caaatatatc taagatacct tccacttatg
    21781 tgctcctttt cgctcgtttt acctattata attaacaaac ggataaatta caacaatttg
    21841 ttaatactcc agcattctct tacgataatg catgtaatcc aagaattcca cctcttcggg
    21901 tactccgtgg tcgcatgtgg gaatatatcc gccgtgtttc ttcatataag gcattattct
    21961 gtctatttct ttgtctattg catcttttcc ggcagccaat atccttttat ctataccgcc
    22021 ggaaattaaa aggtcgggat atttctttcg aacttccact acatcacacc ccgatgccac
    22081 ttcaaacggc gacatataat ccatgcctat ggatttatat aaatcgataa ccgttacggc
    22141 attaccgtcg gtatccaact gaaaatgaag tttcctgttc ttgtccgact gccttttctt
    22201 tacattggca ataagttgct gatagtacgg gaataaaaat tcttttatca tatcgggcga
    22261 aatcagagga ccgtgattgt agcagatatc ctccccgaag tagacttcgt ctaccgttac
    22321 atatctttga tgaacttcac aggttttatc cattacatca aaccatattt ccatacattt
    22381 gtgaataagt tcgggatcat cataaaactt atacagtaaa tcttccggtc ctattaaaga
    22441 ccgcaaatac atatatccgc ctattatatt ggcacatatt atatatccct gcttttccga
    22501 ctccgcggcc ttactcattc tttcttcaag gccttcaaac cttttagggt catcgggatt
    22561 caatctccaa aggatgtttt cttccaaagt ccggatgtct tttaccggat gctctaaata
    22621 tgtgggcata aacccgcttc ttctgccttt aaagtataag acctgtctgc ccgcattatc
    22681 ctgctctatt tcacattcat cggtagtact tataatcttt ctttcaaagg aaggaaccaa
    22741 cggtgcttca caccatccgc aacggcctaa aaaaaatacg gcggacttat caaatccgaa
    22801 caattcatcg gtatcggtgt cgtcatttat ccagccttgc tctttccatt tacccttgac
    22861 ataaaaaccg aactctttct gaaagatggc ggcatctttt ttgaagttat agaagtcatg
    22921 aatcttctga atgtgctttt ccatattgtc tccccgcttg aagtacataa cagatagtat
    22981 tatagcacag ttaagtaaat acttattaag actttaattc aggtatttgg atataaattc
    23041 cgatactttt ttataataca catccggctc tttcgccgaa gcggcaccgt gttccgctcc
    23101 ttttacaacc aatatgtctt tctcacaggg cgcattgtca taaagcggtt ccagcatata
    23161 gaacggaact aattcatcgg catcaccgtg tatgaataaa gtaggtgttt tggacttttt
    23221 aacggcattt aaagggcttg cctgcttaaa atccatcctg gctctggccc gggaaataat
    23281 attcgaaaca taaagtacgg gaaaagccgg cagtttgaaa aaccttttca tattatagct
    23341 gaaaatatcc cataccgatg tataaccgca gtcctctatt gcgcatttaa catgattgct
    23401 caaatcttcc cccgtcgtaa gcataacggt agcggctccc atggatattc cgtgcaacac
    23461 aatcaaggca tcgggatctt ttttaataat ctcctttatc caatcaatca catcgacact
    23521 ctcataatag cccatagtgg tatattgccc ttcactgtcg ccatgtcctc ttaaatcggg
    23581 catcaatatg ttgaatcccg ccttatagta ttgctttgca aactgtgcca tatgccatcc
    23641 gcgtgatgta tatccgtgaa ccggtattac ccatttatga ctccccttat tctcaacaaa
    23701 tacggcttta agattaagat tatcgaaaga agtaagactc cacaccgaag tagtgttctc
    23761 ctttacccac tgttcactcg atgcgaaaaa ctcttttacc tgctcatcaa tatcttcttc
    23821 ttttttattg aaataattcc taggcgaccc gggtatcatg gttatattga acataaacaa
    23881 acccgctata aacgacagga taaataaaat gacaacaaca aatactacaa ttgataaaat
    23941 aatttcaaaa cccatttccc cctccgtcat acattaaaat tatatcacat catcactccc
    24001 atctatcaga tttatgcaaa tttaactctg tatacaatac aatttaacct ataatcccga
    24061 tatttatttt tagtgtgata aaaacacctt tacattatcc ttaatgcctg ttatacttcc
    24121 ggtaatattg atagaggcgc gggagtcata agtatccgtt ctgaccgtac aaggcgggat
    24181 gatgacggaa aaagggacaa ccgccgaaga atacgcagcc ttgtatgcgt ctgtctggtc
    24241 ggttcgtaaa taacggaccg attgtcaccg tcaatttacg gtggagagct gtcacttgga
    24301 aggtttaagt aaaggctcgc acaggcagcc tttttttgat ataatatgac agcaggcggg
    24361 tgaaaaaaat gaaaaaatat aacttggcaa tagtaggcgc cacaggtatg gtgggaagaa
    24421 cctttataaa ggttttagaa gaaagaaaac ttcctattaa gaatatctat ttctttgctt
    24481 cggcgcggtc ttgtggcaat accattactt tttccggtaa agagtatatc attgaggagt
    24541 tgaatgagca ttcattcgac agattgattg atattgccct gttctcggca ggcagtgatt
    24601 taagtaaaat atatgctccc gttgcagcac aaaaaggcgt ggttgtaata gacaacagca
    24661 gtgcgttcag aatggatccg acggttcctc tggttatacc cgaggtaaat aaagaggata
    24721 tcgcaaagca taacggcatt atagccaacc ccaactgctc caccattcag gctttggttg
    24781 cggtaaaacc gttacatgac gcctataagg tgaaaagaat ggtcgtttcc acatatcagt
    24841 cggtatccgg tgcgggcaaa gcgggatgtg acgatttgga gaattgctta aaagggcttc
    24901 cccctgctaa attcccttat tccatagccg acaactgtat tccccacata gattccttcc
    24961 tggataacgg atacaccaaa gaagagatga aaatggtaaa tgagacgcaa aaaatgtttc
    25021 ataccaagga cattttgata acctgtacct gtgtcagggt accggtaatg agaagccaca
    25081 gcgaatcgat aaatctggaa tttgaaaaac agtttgacat aaaggatgta tataaaatac
    25141 tgaataatgc tccgggtgtt aaggtggtgg atgatctttg caatgcaaaa tatccgttgg
    25201 cagccgaagc ggccgatacc gactggacat atgtaggcag gataagaaga gatgataccg
    25261 tggaatcggg attgaatata tgggtggttg cggataacgt aaggaaaggt gcggccacaa
    25321 atgcagtaca gatagccgag gaacttatta aactttggga gaaataacat gttattcaaa
    25381 ggtgccgcaa cggcggttat aacgccgttt aacaaaaaag gaatagatta tgacaatttc
    25441 gctttacaga ttgataatca gcttaaagcg ggaattcagg cgttaatagt atgcggaacc
    25501 accggcgaag catccactat gacaaaaaaa gaaaggtatg atgcgatatc ttttgtagtg
    25561 aacaaagtac aaaagacggt accggtaatt gcaggaaccg gctgcaacga cacaatgcac
    25621 agtgttcagc tgaccaagga tgcgtataaa gcgggagccg atgcaatact tgtaaccgct
    25681 ccctactata ataagacatc aaaaaaagga ctgattgagc acttcagtgt aatatctgaa
    25741 agtgttcccg ttcccatgat actgtataac gtacctacca gaacgggtat gaatattgag
    25801 cccgatgtaa tggtacacct tgcaaaaaac tgcaacataa caggtgtaaa ggaatgtaat
    25861 ctttctcagg ttgcggatat tatccagact gcaccggaaa actttaccgt ttacagcgga
    25921 gacgactcca taaaccttca ggctctgtca ttaggcgcaa tgggagtgat atcggttatg
    25981 tccaatgtcc tgcccgcaca tgtggtgaaa atgaacaaac tgttcttgca aaacaaaata
    26041 aaagaaagcc gtgagctttt ctataaaacc aagaatcttg taaaactgct ctttattgaa
    26101 accaatccga tacccgttaa aaaagcaatg cagctggcag gttatgatac cggactttta
    26161 aggcttcctc tggtggaaat ggaagagaaa aacgcaaatc ttctcaaaga agaactgatt
    26221 aatctgggag ttataaaatg aacatattac ttgtaggctg ttacggaaag ttggcaagtg
    26281 caataataaa agcatgttcc gggaaagagg acatgaatat agcatgcgga gtggatcttt
    26341 cggtaaaaga taatcctcac ttccccgttt ttaccgctgc aaacgatata ccgtcatcaa
    26401 tggacattga tgcggttatt gactgttctc atccttccgg agctgtatct accgtccggt
    26461 tctgtaagaa atacaaaaag cctcttgttt atgcaacaac cggtctggat aaagagcaaa
    26521 tgaacttact tcatgaatta tcaaagcatg ttgccgtttt ccagtcggct aatatgagcc
    26581 tgggagttaa tcttctcata aaactctgca aacaggccgc aaccttctta ggcaatgact
    26641 ttgatataga gattatagag aaacaccaca atcagaaaat agatgctccg agcggtacag
    26701 ctctcaaaat agccgatgaa atcaaccaat cgctgaataa ccagatgact tatgtttatg
    26761 accgtcagtc aaaaagagaa aaaagagata aaaaagaaat aggcatacat gcagtaagag
    26821 gcggcaacat tgtgggacag cacgaagtaa tctttgccgg gcaggatgaa acaataacaa
    26881 ttagtcacgg ggcattttcc aaagatatat ttgcacaagg tgcattaaga gcagttcttt
    26941 ttataaaaga caaacctgcc ggattatacg gtatggatga tttgctttag acactattaa
    27001 tacaatttaa ctttgttaat ccgttgctct ttcattatcg gatctaagtg tttttccaat
    27061 gcaggccatg taaagaaatt aataatatac gaagacagtc cgaaaagaac ggtggccata
    27121 accactgctc cgaacagtat atgtatctta aacggaagta tcgttaaagc cgcaataccc
    27181 gcaataatca aaagattggg aaagaatctg aggatggcaa acacataaga gtatctgtaa
    27241 agcttccaga tggataaatt gtactccaca agaatctgat atatgtacat gtgcatcatg
    27301 ataaataaca gcgaaaatcc gccaataatg agcaacaggg ctgttttcca gaaacctgcc
    27361 tgtaaattca actgataaaa cctggctgca atacccagac aaacggttat aaccgtggaa
    27421 attgccgtaa ccgccaggga tttccagaag aactttttca ttccgtcacc gaagtcggaa
    27481 gaaaagctgt catctcttct tacatagctc ctgaaaacta ccgcaagccc cgagaccgcg
    27541 ggtccgaatg ccacaacagg tattgtaaag aatactaagg tataagggat tcttaaaatc
    27601 agtattgccc ataattcacc gaaattggag tatccctgct gataatcgga aacatcaatg
    27661 ggaaaataaa tctgtgatac aaagaatgcg tagaagacta taaagagtgc gtatataccc
    27721 acacatatca tattgtattt ggctatattc cagaaccttg tctttaagat ttccataaac
    27781 ttcataaact tggtttccgt aggtttctgt tcttggtaat ttaccttttt tttgtcactc
    27841 ataagataac tcctcgcaaa gtaacattct atacattttc ccgatattta tcaagcaaaa
    27901 cggtaacaaa accctccata ccctgccctg acagtcaatt tttgcgttga caaacaatta
    27961 catggtagtt ataattccta agtaagcggt catggcgaaa ctggcagacg cgtacgtttg
    28021 aggggcgtat gggcaaccgt tcgggttcaa gtcccgatga ccgcaccatt ttatacaatt
    28081 tcaagtctcg tttttaaaaa tgttgacaaa tgcacccaat gggtgcattc tattaatgag
    28141 gtgataaaga tgactatacg gaaaatgaga actgaattag gggatactca aagtgagttt
    28201 gccgcaaggt acaacattcc gtttcgaaca atccaaaatt gggaaaccga ggttcgcaag
    28261 cctcccgaat atattattga actgcttaga cagcgtgttc gagcggatct tgccaatcga
    28321 cgcactgtga acttgccgga gtattgcccc gaaaagcgta cccttcctaa gcgaagtgat
    28381 tttatcggct cactttcatg gctgaaagct gtacaagctt gcctgggtga tgacatagtc
    28441 tttgctttag accaagctct aatgtgtcag ggaaactttg gtggtcgtag tgatgaatat
    28501 tcagtatggg tttacggaaa tgacgcagtt accgattata acggagtaat tgtgattggg
    28561 aaccgggtta gcccttatga tgtggagagc aagaacggtt ttaggtatac taatttaagc
    28621 agaactattt cagatgcatt tgccaacgaa tcaattttag atatgcaggg tgtaaccgag
    28681 gctgttagtc gctactatta ttctaataac gaaaaatttg atgggatttc tgtcgccccg
    28741 gagtatcagg aaaggtttga aagactagct agagaggcaa ttgaatatta caaaacaaag
    28801 agataggctg atgaagatat caccatttct aacatacata acatcaacaa atgtgttaaa
    28861 aatcaataaa atcacacttt ttggcacata actgcatttt actatgcttt tcaagagaaa
    28921 tacgtgttgc ctactcacaa gttttcaggc tataaaaaac atctgaatta catagaccgt
    28981 tgtaaccgcc ttgttaatcc aatataatta tggtatcgaa attcgggagg atattttatg
    29041 acgagaaaat ccattttgtt tgtccaagtc ggaaaagcgg agctggttga cataccttta
    29101 ccggaattaa aaacaaacga ggtactggtg cgtaccgcta tttcgactat aagcagcggt
    29161 acggaacgcg ctaatctgtg cggggattta aatgtttcgc cgatgcaacc gacccttacg
    29221 gttgccaaat ttccgcgctc atccggttac agcacatcgg gtacggttga ggccgtaggt
    29281 gcggctgtga caaaagttgc cgtcggtgac cgcgttgcca tgtgctggtc atgccacacc
    29341 gatttgaatg ttctttccga aaacctggta tataaactgg acgacaatat ttcatttgaa
    29401 acggcggctt tgctccatat cggaactttc cctatggcgg caatccgcaa aacacggttg
    29461 gagtttggtg aaccggctat cgtcatggga caaggcatac tcggtcagat tgccgtatta
    29521 caactgaaag tcgccggtgc ttcaccggtc atcgcggttg atctggttaa agaacgccgt
    29581 gatattgcct tgaatctggg agctgatttt gcgctggatc cgaccgattc tgaattcgta
    29641 aacaatgtca aggcaatttg tcctttaggt gttcccgtag ctattgaagt caccggcgtc
    29701 ggcaagggat ttgatcaagt gttggattgc atggcacagt tcggtcgtgt tgctttactc
    29761 ggttgcaccc gtaacagtga tttcaccatc gactactacc gaaaaatcca tcggccgggc
    29821 atcacggttg tcggtgctca caccatggca cgtccggaag caaactctta tccgggctat
    29881 tggacacgaa ccgatgatat ggaagcgcaa cttcgtttaa tcaaatacgg tcggttatcc
    29941 tatgacggtc tggtcaagga caccagaagt cctgttgaag caccggaggt ttttaaccgg
    30001 ttggctaacg atccgcactt ccctattact caatttgatt ggagaagact tttatgaagc
    30061 cgttcagaat cgcccaaata ggcaccagtc gctggagcca cggcaacgaa tgtttccgtg
    30121 ccttgaagaa taatccggac gtatttgaga ttgcaggata ctgcttcccc gaaaatgagc
    30181 aggaaaagtt cccaaagcaa atgaacatat tcgagggtta ccgcgaattg acgcttgacg
    30241 aaatattaaa tgatcccact atcgtcgctg ttgcaatcga aaccgaggaa aaatacttga
    30301 ctaaatatgc actttcagtt gtgcggcacg gcaagcatat ccacatggaa aagtccggcg
    30361 gtttggattc tgcggaattc gaacaggttg caacaatcgc aaaaaccaat caaacggtgt
    30421 ttcataccgg ctatatgtat agatataatc cttatgtaca ggaactgttg gaacagatta
    30481 aatgcggtga actcggcgaa attatcagtg ttgaagcgca gatgaactgc tatgaaccgt
    30541 tggagtgtcg gaaatggctg gaagattatc cgggcggtat gatgttcttt ctggggtgtc
    30601 acctgattga tttaatttac accattcaag gtcaaccgaa ggcaatcatt ccgctgaaca
    30661 aatccacagg tctggacggt gttacctgca aggatttcgg tatggtaata ttcgaatatg
    30721 acaaaggcgt gtcttttgcc aaaacatcag cggtagaagg cggcgggttt gagcggcgtc
    30781 agttggttgt ttgcggtagc aaaaaaaccg tggaactgca tcctttggag cggcacatag
    30841 aaaacagact gctgacaacc ggtcgttaca tacgggaaaa ttccactctt tggaatacag
    30901 tccatgagca ggagtttgcc gaaccgtttg accgatactc ccctatgatg ttgagttttg
    30961 cacaaatggt acggggcgag aagaagaatc cctatgatta cgattacgaa cttacactgc
    31021 atcagcttgt gataaaggcg tccggaggat aaaaaatgct tttgattcca agcggtagca
    31081 cactcggagc tttacggaca aggaagaaga actgaatgtt cttgaaatat atagacgctt
    31141 ttttacagaa aatgtactta atatggaaaa aacccttgaa caacagtctc accagatgat
    31201 aaaataaata tgcggaggat acaaacatga ttagaactat cagaattttc aaaataataa
    31261 taatgatctt gttttttcca tttttcgtaa ttttagatgc ggctaaaaac tggaaatctt
    31321 aatcaatttt aagaagaaca cctgcatatg gtatatggtg agaagaaccc ttatgattac
    31381 gattacgaac ttacaccgca tcaacttgtg ataaaggctt tcggaggata aagaataatg
    31441 cgcagaactg ttgaaatcat aaaattgata cttataatat tgttctttcc tttttatgtt
    31501 attttattag cagccggaat aatcaaatca tgattaataa cattcatatc ttactgcacg
    31561 cccgcattcc gtaggcgttt tttattcttc gtcaaagata atgaggcaaa attacatttt
    31621 ataagattcc gattattctt ccggtattta tccgatatca tacctgctta atatcaccct
    31681 cttctttttt cagaaaacag tgtcttaaat tgataattaa cataatttct atagtataat
    31741 acatctgacg ggagtattta atatggaaag aaacatatct gcaattatat cggaaggcaa
    31801 aacctttatc ggcatagagt tcggctctac aagaatcaaa tctgttttaa ttgatgatgc
    31861 attcaatgtt cttgcttcgg gaaacttcga ttgggaaaac aagcttgaag gcggttactg
    31921 gacatacggg aaggacgaaa taattgaggg gatgcaaatc tcctatcgga atctcgctgc
    31981 cgatgtttat gaaaagtacg gcgtaaaatt aataaagacc gcagccatag gcatatccgg
    32041 aatgatgcac ggatatttgg catttgacag tgaagataat ctgttagttc ccttccgtac
    32101 ctggcgaaat ataactacgg gagtagctgc aaagaaattg agtgaaactt tatcattcaa
    32161 tattccaaag aggtggagca tttcccatct gtatcaggca atcttggata aggaaccgca
    32221 tgtagataaa attgaccata taaacactct tgcaggacat attcattatc ttctcacagg
    32281 gaaaaaagta gtgggaatcg gtgaagcgag cggcatattt cctttatcgg ggaatgggta
    32341 caacaaagaa atgctgaaaa agaccgaaga gctgtttttg gcaagcggtt actctctgaa
    32401 attggaaaaa ctgctgcctt ccgttaagaa aaccggggaa acggcaggtt atcttaccga
    32461 aagaggtgcc aaattattgg atcccacagg aacactgcaa cacggaattc ctctttgccc
    32521 tccggaagga gatgcaagca caggcattgt tgccaccaac agtatccgtc ccggtaccgg
    32581 caatatcagt gcgggaacaa gcatctttgc aacagtggta atgaaatctc tgctaccaat
    32641 caacaatcct gagattgata taatgtccac tccggacggg tatcccaccg ccgtgattca
    32701 ttgcggcaac tgtaccggcg atatcgatgc atggatgcac atatttcaag aggttctcaa
    32761 agcaaccggt tcttccgttg aaaaatccaa gctctttgag actctgatga ataaagccat
    32821 ggaagctgat gccgatctcg gaggtttgat gaatttcaat tatatctccg gtgaaagcat
    32881 tacaggaatt aaacccggag taccgctttt cctaagacag ccgggagcaa ggtttaccct
    32941 cgcaaacttc ctgcagacac atctcttttc cgctcttgcc acactcagga taggtatgga
    33001 atactttttc aaagaaggtg tgaagtttaa cagaataatc tgccacgggg gttttttcca
    33061 ctcacaagcg ggagccaaag caatggctgc tgcacttgat tctcctgttt acctgtataa
    33121 aaccgcaagt ctgggcggac cctggggcat ggctttactg gctgcctatt cacaatctga
    33181 agagagtctc acacttgccg attacttaga caataaagta ttcaaaaact tcgaattgat
    33241 tcaagtgctt cccgaccggg atacggtcac aagctttgtg tcttttttag aaaaatacag
    33301 aaaattcctc aaagtagagg cagatgcatc gaggtgtttt gatgaataac ttagcacaat
    33361 taaaacaaag agtttatcgt gccaatatgg atttagtcaa acacgatctc attgtcctga
    33421 catggggcaa tgccagtgcg atagacagag aaaaagagct ggttgtcatt aaacccagcg
    33481 gaatagagta tgataaactg caaccggaag atatggtcat agtaaacatg gacggcaagg
    33541 ttaaagaagg cagtctcaag ccctcgagtg acatactgac tcatatagag ttatacaaag
    33601 catttcctga aatcggcggt gttgtccata cacattcctg ttttgcgaca gcttttgcac
    33661 aggccggaaa acctgtctta cctttcggca caactcatgc cgattgtttc ttagggccta
    33721 tcccctgtac ccgcttttta aaagaccgtg aacttgcgga ttacgagaaa aataccggaa
    33781 aagtaattgc ggaaacattt acttctctgg attaccgtac aatacctgca gcattggttg
    33841 ccggacatgg cccttttatc tggggcagga cacctgagga aagcgtactt catgctgtga
    33901 cacttgaagc ggtggcaaaa atggcttttc tgaccattac cctgggcaga gaacaatgta
    33961 taggacagaa acttttagat aaacactatt tcagaaagca cggaaaaaat gcttattacg
    34021 gacaagaaaa aaataaatga aacatatttt ctgctcgtaa aaccggaatc tcaattgaga
    34081 aggtgtttaa aaacaggtta aagaactata aaagttaaaa ttccataaaa tatatcggca
    34141 ttacggttgc atttatggtg tcattatggt gtcacttgaa taaatatatg tgacaaagat
    34201 aagggcaatt caaataacaa atgaaataat ttcagagtgc tgtatttatt aggtaattta
    34261 acggttgctt ttaagttcca tctccatatc caatagcttt tctttcaaat caatccccgg
    34321 tgcataacct gttaaacaac cttttttgcc tataatcctg tggcaaggca ctattatcat
    34381 tacaggattt ctgttgcatg ccatacctac tgccctgctt gcttttgcat ttcctatgag
    34441 tgctgcaatt tcaccgtagc tccttgtttc tccgtacgga atattcagca gttgctccca
    34501 taccgatttc tgaaactcgg tgcctttcag tgaaagcttt acattgaaac tttttcgttt
    34561 gccttgcaga tattcggcaa gttgcctgaa tgtttcatct ttcagttttg attgcgatga
    34621 atggctacaa tcttttgctg tacaagtcga tacaccgcat ataccttcgt tgtcttccga
    34681 tatattgatt ctgcctatct tcgattggta acagcaaacg gttatcatta tataatctct
    34741 aaaaactcgt aggtcggtaa agtcggaaat ttattaaagg gcaaagtctg gtaccaataa
    34801 gccaccgaag aaacatcatc ctgcagtgca agatatttag tgttacttct ccaacctaat
    34861 gcctgcatgg taacccttaa gtctttttca aatcttacag ggtcggtaat atgccatctg
    34921 tacatggaaa aacgctgttg accttgcgct tctttttcat atcttacggg ataaaatccg
    34981 ctataggcgg tggaataatc taaatacctg tcattttcca taaagcagta tgctccgcag
    35041 aaatagtctt cggtaccggt accgcagatt gtcgggaatt tatcgtctcc gtccataaag
    35101 aacttgatct ccccttctcc ccaccatccg ttgttattgg ctccccatgt catatatgtt
    35161 ccgacatagt gtcctttgcc ttttatatta tcgataatag tgtaatcggt cttgtattta
    35221 acgggatttt ctcttctgaa ctgagcaaag aaatatgcac agtcatcggg tatttcagtt
    35281 agtatatagt caatctgata gtaatgatat gctcgtttcg gacctacatt ttccagtgtt
    35341 atcttacagt gtttacgaaa aggcatttcc caataacagt taaagcctct gacactgttg
    35401 ttgcatacgg caagtgaatt aatcggtatg tgaataggaa gaaaagcatt ggcaaagaaa
    35461 tcacctatgg gacattccac cgacggatat tcacagttat cccagtagaa ccgtataatc
    35521 gtatctcttc ctgtcccgcc gcttgtaatc catatatgct taattgctcc cggtccttct
    35581 atatcggcaa tttcaaaaag ttcgcccggc tgcaatacta ttgaaggaga aactttccag
    35641 cccaaaccgc ctaaatccga tgcgggatat gcaccggtac cttccgttgc cataccgcct
    35701 tttccttttt cgcctttgaa attttccgca cttatcgatc tggattgcgc atccgacaat
    35761 ctgctaagac ttgataaatc acttcctaat ccgttataca tttgctacct ccgatatatt
    35821 taaatatgta gtttttttat aagttcattc tgcagttttt tattgcctgc aagcagtacc
    35881 aatcctatta tcacgggaat tatggatgcc gcaacaatat atgaccagga caattgtata
    35941 tcagaatata tgtagtaatc tattactccg tccgtaacaa ggcaaatgat aataaccgca
    36001 atgagcgata ttgaaacggt tttcagtttt ccgggttttt tctttattac aatcagaatt
    36061 ataagataca aaagcgacat tacggttatg catatcggta aggcgataag taagaaccag
    36121 cgcattcccg ataaggccaa agctatcagc agaagatata gcgtggcgga taaaaacagc
    36181 aggaatatat gcaacagcgg tcttttcttc tttatcgcca tggggaatat gaaaatcatc
    36241 cacaacaaaa gacttccgcc gaacacataa tacatccagt atactcccgg ggtatatatg
    36301 aggttgatta caaggcaagc cagattgggc acaagcagaa tcatggtcag aagtatagag
    36361 aaataccctc tgttcaactg tgatatttcc aacttctctt tcggataggg cggaatttca
    36421 tcgggcagct ccatcattac ctttgtattg cacaaaacac acttttttgc gctattatcc
    36481 agttccactc cgcattttac acaataaggc atttactctc acctactctc tgttactttc
    36541 aattttaacc ggaataccct gtttaatcaa atatgtgaaa aaggttctct cgatatcggt
    36601 ttcctgtatt ttgctggtaa aattcaacac cagtgtatct tcataactga caacggcaag
    36661 atacggagtg cgtatctttg ccggtcccag tataaattca aatctgttta caagttcctt
    36721 catttcggga ggcactgtta caatccctat attggatata acagtggtta tcggtctgta
    36781 acctatggtg tcatgagcta aggacattat gaaattcttt ataaacagag gaactattct
    36841 tataaataaa ttcctttcca atattacatt ctgagtcatc atggcattga gcactttatc
    36901 attcagattg gctttcatga aatactgaat ctgccgcaac atttcctcca tcgtatattc
    36961 acccaatatt gtgtctattc ccgggttcag aaacgtggag aaattccttt tggtgttgct
    37021 tttgaagtaa tttctcagat taacggggat agataccgca atcctcgtag gttttttgta
    37081 cagtccttcc ttttcctttt gtttgtagaa agaataaagc agaatagctg caagatattc
    37141 cgttatcgtg gcatggtgct ctttggcaag ctgctttatt ttctccagag aaactattcc
    37201 ggtgattatg gatatatcca aatcatccag tccggtaccc ttcatatgat aggctttagg
    37261 ttctttcggc cgcccccgtt gtctggaatt tgcatatttg acgaaagaat cctcatattc
    37321 ttccttatcg ggtttttcct caacatccag tattccttcc cctgtcggta tttcgtagcc
    37381 tttgagtgta agatagcggg cagtcagtgt tttaagaaac accatagctc cggtaccgtc
    37441 ggttaaggaa tgaaagaaat ctatggctat tcttttgtca taatagaaca ctctcagtaa
    37501 gaaaccgtta ttgtctttcc atcttatggg tgcacacgga ttattgatat cgggttttac
    37561 ctgcagtctc ttccttatat tactttcaaa atagtaccag aaaatacctt ttcccatccg
    37621 tatattgaaa ctgggaaatc ttttgattgt cgcaacaaga gcctcctgaa gaataacggg
    37681 atctattttc tcgttcaaag taacgctcaa tcggaacatc cccgaccatc ttcttgtgtt
    37741 ggcggcggga tatatcttag cggcgttatc cagtttaaac cagtgtctgt actttttctc
    37801 ttctattttc cgcaacattt cttatatttc ttaccgctgc cgcacggaca aggctcattt
    37861 cttcccacct tatctctcac tgcggtgtgg tcggctacat actgcttcct tattctttct
    37921 ctttcttcct ttgacaaaat atcttcccat tcatctaaag agtagagcca tttggctttg
    37981 gcgttatgca tattgtaata taatttctcg aagtctattt cacaattaag tgcggtatca
    38041 ttatttaaag tatccaaatt cacgttcttt ttcagtgatg tattgattcc gtccataaaa
    38101 ccggcaaaaa cccagtcttc cataccgaac ttttccgcca actccgctac cgttcctttg
    38161 agatgcttat ctttctcagc cagtatcgcc ttatatgcct cggtctcctg ttctaagtag
    38221 gtattataga attgtgctgc atcgtcggac ttcagtttgt cgttccattt atcatatatg
    38281 ttcatatatc accctataat ttattctcta tctctttaac tatcctgttc tggaattcct
    38341 ctaagaacat ggttccgata tcccctattt ttcttactct tacggatatc ctgttatttt
    38401 ccatctcgtt gtctcctacg ataatcatat agggaatttt ttccagttgc gcttctctta
    38461 tcttgtatcc tatcttctcc gaacggaaat cacattccac acggatgtct ttggcaagca
    38521 gaatatcccg tatcttctgt gcataatccg cctgcctgtc tgtaagagga agtaccttta
    38581 cctgaacagg tgccagccat acagggaatg ctccggcata atgctcggta agtatggcta
    38641 taaacctttc aatggaaccg aacaacgctc tgtgcagcat tatgggtctg tgtttgtttc
    38701 cgtcggctcc catatatgtc agatcaaacc gctcgggcat ctggaaatcc agctgtatgg
    38761 ttccgcactg ccatgtacgt cctatggaat cctgtatatg gaagtcaatc ttaggtccgt
    38821 agaacgcacc gtctccggga ttcagctcgt acttcatgtt caaggactcc aaagcatcct
    38881 gcagcgcttt ttccgctata tcccactgtt ctttggttcc catggagttt tccggttttg
    38941 tggacaattc cactttgtat tcaaaattaa aaagagcgta aatctttttc atgagattga
    39001 taacacctat tatctcatct ttcatctgct cttcggtcat aaatatatgt gcatcatcct
    39061 gtgtaaagca tctgactctc ataagaccgt gtaaagcacc ggacaactca tgacggtgca
    39121 caagtcccaa ttccgccgtt ctcaagggca aatccctgta ggaatacatg cggcgcttaa
    39181 aagccagcat tccgcccggg cagttcatgg gttttatggc ataattgcct tcatctatcg
    39241 ttgtgaagta catgttatcc ttgtaatgat cccaatgtcc cgatttgtgc cacaattcct
    39301 cattaagaat aatcggtgtg attatttcac cgtaaccggc tcttttatgc tctcttctcc
    39361 agtaatctat taactgatta cgaatttcca tacctttagg gaagaagaaa ggaaaaccgg
    39421 ggccttcttc aaatatatca tacaaatcca gttctctgcc cagtttgcgg tggtctctct
    39481 ttttagcctc ttccatcttg tatatatatt cgtccagcat ggtctgtgaa gggaatgatg
    39541 ttgcatacaa tctttgcagc atcttgttct tctcgtctcc gcgccagtat gcaccggcaa
    39601 cagacataat cttagcaacc tttacccttc ccgtagaagg cagatggggt ccggcacaaa
    39661 ggtctacaaa atcgccttgt ttatagaagg agatttcttc attttcatcc aattcactta
    39721 tcaactcgta tttgtacggg ttgtctttaa caaatttcaa tgcctcgtct ttgggtaatg
    39781 taaacctctc gataggataa tccgccttga ttatttcttc catctttttt tcaatcaatg
    39841 caaagtcttc atcggtaaaa gtatgttccg tatcaaaatc gtagtaaaag ccgttgttaa
    39901 tagccggacc tattgcaagc tttacatcgg gaaacaacaa tgtaacggca tgagccatta
    39961 tatggctgga agtatgatga tatacatgag caccgtcttc atcttcaaac ttcaataaaa
    40021 gtaaatcggc atcattctca ataggctcgg tcagtccttt aacttttccg ttaacctttg
    40081 ccgccactac cgcttttgcc aggcctctgg aaatatccgt tgcaaccgat aaagccgtaa
    40141 cagcattctc atattctttt atagatccgt caggtaattt tatctttatc atatcttttc
    40201 ctcctgtacg tcaaacaaat ttaaaaatgc cccgaatata tcattcaggg gcagtgtatc
    40261 tttccgcggt tccaccctga ttagccgtaa agccatctct aaagatcgta tcgtaatctg
    40321 aacggttctg ctcggggagc ggtctttcgg tgttttgtaa aaggatttca gccatgtcct
    40381 ttatctctgt gggtttcacc tactcttttc ccgtcatcac agcattattc gctatttcaa
    40441 gattctatca cctaaaaaag cattatgcaa gatattttta taatccatca tataattcgt
    40501 atagcgtata gttgataatt tgaattaatt caatgttttt aatatactat atatggaaag
    40561 gttcacaaaa atgatgataa ttcttggagt aatatttttc atatatcact tctttgtagg
    40621 gttaattaag ggaaactgac agctgactat cagttattat tcctcattgt ctgtaactat
    40681 tcagttattt ctatactaac ttctccttat caaaggattt cagccattct cttgccactg
    40741 atgccacttt cttaagtgct ccttcttcaa aggggctggg taaacctgct gatttaacaa
    40801 ggcctgtgaa agtgtccttg ccgcctctgg aagtcattgt gtaatattta tcccaagctt
    40861 tcttaaagtc ttcctgtgat tccgcccagt aagccaatgc aacggtctgt gcaaggcaat
    40921 aatctatgta atagaaagga gcggagtaaa tgtggtgctg tctttgccag taccggccgt
    40981 cttcaaagaa aggcatgccg tccgtattaa ggtaaggcat atactctttt tgcagcttct
    41041 tccaaacttc ttttctttga tcgggtgtaa ggtcgggttg ctcatatact atatgctgga
    41101 aatagtccac aatagttccg taaggcagga atgaaagggc actgccaaga tgtgcatact
    41161 tatacttatc ggtctgctct tcaaagaaaa gatgcatcca cggccatgta aagaattcca
    41221 tggacatgga gtgtatttcg caagactcca ttgtcggcga tctgtactcg gatattttaa
    41281 aatctcttgc aagataagat gcgaaagcat gccccgcttc atgggtcaga acatcaatat
    41341 ctcccgatgt gccgttaaag ttggcaaata tgaagggcgc tttaaaatcc ggtatggttg
    41401 tggaataacc gcccacagct ttgccttttc tgcttattac atcaaataac tcattttcca
    41461 gcatgaagtt catgaactcc tcggtttgcg gacttaattc ggaatacatt ttctttcctt
    41521 gggcaaatat gtgctcggga tctcctttgg gtgtagcatt tcccgataag aattccaaag
    41581 gatcgtcata aagcatgaac ttatccactt taatcctgcg ctgctgttct tttttcaact
    41641 cgctgacaat gggcactatg tattttttca catatccgcg gaatcgggcg acatcctctt
    41701 ttgtataact gtttctcatc atacggtaat atcccaattc cacgaagttt tcatagccca
    41761 tttctcttgc catatgtgtc cgtatcttga ccagttgatc ataaaggttg tccaaagcat
    41821 cgctgttttt taagtaaaac cccgcttcgg cttcatatgc ggcttttctt accgctctgt
    41881 cggtactctg cttatacttc cctaactgcg acagattgag tactttaccg tcaaaatcaa
    41941 tctgtgcact tgccagaagt ttctcatact cgttagtcaa cctgttttct tcctgcatat
    42001 gaggaataat cttggggtcg aaagatttca tataaatctc aatattaaga aacagtaatt
    42061 cacccagttc ctttttaagt tgctccttaa acggcgaatt atataatgta agtttaaatt
    42121 gcaaatccag attctgtacc atgggcatta tttcatcgta gtactctttt tcccccgcaa
    42181 aaaactcatc ttcggtattc aaagtgaatt ttatgtaagc taaagagcct gccgtggtta
    42241 tcttatcgga atactcattg tacttataaa atgtgtccaa cgcttctttt gcactcttag
    42301 cattgctcaa ttttgctgta agggccttgg ctttagcttc aatctcctgg taagttactc
    42361 gtttatacgg catttgtgaa aatttcattt ttcctctcca atcatatata ttgcaacctc
    42421 gtaaggtctg agaaactcat tgctgtccgg gtagttggac agtatttttt tatacttttt
    42481 atctcttaca tcgatctttc tcttcttatt gctatcaatc agatttatct caatgtagaa
    42541 gcgggcatct ttgtcttttc tttcataaca gaatgtatcg gcatcgttat cggtcaggat
    42601 aaagtctccg tatatgagag tttgattctc ttttctgaag gatatcagtg acttcatgta
    42661 attcagtacg gagaatgtgt cctttatctg acttttagca ttatacctca tatagtcgtt
    42721 ggagcaatct atccatggtt ttacttcgga gaatccgccg taagcggcat cttcccactg
    42781 catgggtatt cttgcatggt ctctggtacc ggccgatatc ttcaggattg cttgctcctc
    42841 tgataaaccc tgctcaagat actcgttaaa catatttatt gcttccacat cgcggaaatc
    42901 cgatatctcc ttaaataccg aatcaatcat ggctatttcc tgcccctgat aaaaatacgg
    42961 ggttcctctc aatgtgagca tgagtgtgat tatgagtttt gctaccaaat cacgtttaga
    43021 ttcatctttt gttatcctgg atacaaatct ggggttatca tgattttcaa aaaccagtgc
    43081 cggccagcag ccgtctttga aatctctttg ccatttgaca agaaggggtt tcagttttct
    43141 aaggtcatat ctgtaggaat cgtgtctgga tttgccgggg ttattgaaat ggtcgaagtt
    43201 gaatatctga tctaattctt tacgcgactc ttccgtcatg agtttggcag cttccagtcc
    43261 tatcccggca ccttctccca cggtaaatgc attgtgcttg tcaaaagtat tttttcgcat
    43321 ttccctaaga tactcatgca gtttgggtcc gtaaaacata tgctcatacc ctattgcccc
    43381 taccagccct ttcaatgccg gtaaaccgtc gggtaatccc ggtgttttgg atataaggtt
    43441 gattacatcc agccggaatc cgtccactcc tttttccagc caccaattta tcatatcgta
    43501 aatatcttta cgcatatttt cattttccca gttgaggtcc atctgttttt tggaaaaaac
    43561 atgcaaagcg tattcatcca ggtggggata atagttccag gcggaaccct tgaaagcgga
    43621 agtccagtta ttgggaggtc cgttattctt gccttttctg aatatatagt agtcgtgata
    43681 tttcgaattc ttatctttta acgcttcctt aaaccactca tgctcatccg atgtgtgatt
    43741 tataaccagg tccataatga gtttcatacc tcttttatgc acttctctta aaagaaggtc
    43801 aaaatcggcc atatcgccga attccgtcat tatcttttta taatccctta tatcataccc
    43861 gttgtcatcg ttgggagaat cgtaaacggg agataaccat atggcatcaa tgcctatgta
    43921 tttaagataa tccagtttgg atactattcc gtttaagtct cctattccgt cattattcga
    43981 atccttaaag gacctcggat atatctgata aaacacggcg cttttccacc atgtcttttt
    44041 taactccgat gtaggacttt tatgcaaatc aggctcacgg ttaagcatat cgataagaga
    44101 atccacaaaa tcttcggtta tctgattctt ggtaagacga ggtaatctgc ccacccttat
    44161 attccccaca ataggattct taaacagaaa tgctccttta ccggacatat atgcaatcaa
    44221 gtccaaaaca tccttgccta aaggggcatt aaacagtgtc tttatcctgc tgtttcttgt
    44281 atacatttgc attctccctt ctttttaatt ttaacactat gaccgaatgt aagcaaacgg
    44341 atattacacc taatcgtaag attgtcagat aataatctat attcaaaata catttgagtt
    44401 taagcggaac ctttaacctg aatgaacata caggtaagaa caatcattcc cctatttttc
    44461 tcacttttat atccccgctg cctatctcgg attttaacag ataatagtcg ggcggtctgt
    44521 tccatgtaga tgttgacaaa ataatataat catccaactc cgtaagatat agcgggttgt
    44581 agcatttctc ttcaatatca atcttaacac tgtctatcag cttgtattgt ctgtcaaaga
    44641 tgtaaaacat aggtgaagta acggaatcct ccgtttcaaa gtactgacat gcgtatatgt
    44701 actcgtcatt atacgagacc gaataaaaac cttcacctgc aacatccgca acatgctcgg
    44761 tttttcttgt ttcgtaggta tattcataga aaccttcacc gttcagatac ca
//
LOCUS       DAOSOM010000015        44441 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_899_length_44441_cov_6.574055, whole genome shotgun sequence.
ACCESSION   DAOSOM010000015 DAOSOM010000000
VERSION     DAOSOM010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 44441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44441
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_899_length_44441_cov_6.574055"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PLF14_04980"
     CDS             complement(<1..180)
                     /locus_tag="PLF14_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70682.1"
                     /translation="MSKVKIGVFGVRRGSVVIKYCRTQSDAQLVAVCDSWEQGLTDMK
                     KELGEDIAYYTSFDDF"
     gene            complement(214..1449)
                     /locus_tag="PLF14_04985"
     CDS             complement(214..1449)
                     /locus_tag="PLF14_04985"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT70683.1"
                     /translation="MSKVKIGVFGVRRGSVVMKYCQTQPDAQLVAICDKWEQGLTDKK
                     KELGEDIAYYTSFDDFINHDMDAVVLANYANEHAPYAMRCMEKGLHVLSEVLPCQTMA
                     EAVQLVETTEKTGKVYAYAENYCFMAAPYEMRKLYRSGKLGEFEYGEGEYIHNCESIW
                     PRITRGEKHHWRNNMYATFYCTHSIGPLIHITGLRPVSVTGFELPFNARMERMGAKSG
                     SAGMEIITMENGGIIKSVHGGCSKNSIWFCIYGTLGRAESSREDAQYGAYSRIFTNLD
                     RYEGECFDRPDTYCPEADEPSIVRKWGHGGSDYHVMHNFVDRILGNTEADTIDIYEAL
                     DMFLPGMFAYRSVLAGGIPMSIPNLRIVSERDKWRNDTWCTDPAVAKDQLVPSYSKGN
                     PDIPDETYQRIYEKWINGV"
     gene            complement(1521..2759)
                     /locus_tag="PLF14_04990"
     CDS             complement(1521..2759)
                     /locus_tag="PLF14_04990"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT70684.1"
                     /translation="MSKVKIGIFGVRRGSSVVKYCRTQPDVQLVAICDKWEQGLMDKK
                     KELGEDIAYYTSFDDFIKHDMDAVVLANYANEHAPYAIRCMERGLHVLSEVLPCQTMA
                     EAVQLVETVEKTGKVYAYAENYCFMAAPYEMRKLYRSGKLGEFEYGEGEYMHNCESIW
                     PIITRGEKHHWRNNIYATFYCTHSIGPLIHITGLRPVSVTGFELPFNARMERMGAKSG
                     NTGMEIITMENGGIIKSLHGLGPSKNSIWFCIYGTLGRAESSREDAQYGGHGRIFTNL
                     DRYEGECFNRPATYCPGADEPSIVQKWGHGGSDYHVMHNFVDRILGNTDADTIDIYEA
                     LDMFLPGMFAYRSVLAGGIPMDIPNLRIASERDKWRNDTWCTDPAVAKEQLVPSYSKG
                     NPDIPDETYQRIYEEWINGV"
     gene            3069..4265
                     /locus_tag="PLF14_04995"
     CDS             3069..4265
                     /locus_tag="PLF14_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70685.1"
                     /translation="MKYPDILFYGERGILNGILMDIYRNRESCKKVNKFFGAIKLLNR
                     KEVPWKNITSCKWLVEPDLSEFGAPDLIAVLESDGKKYALFIEAKLDKYTKSSFHLPQ
                     KVSPASVKRYSSRLNVQLSFRYRFVEAFKEIPKDNKGHNTQEIIETSQHWDEKMRKLA
                     KLPVITEMKNFLKGVEEYYFIALTNDFSSEKVISEIEKKQNIHPPLYDGSYEEFKKHQ
                     SKFGVLTYRDLEDIIVGKCNGNNNEELSSFCKACKMMDMKIANDCNELQTNTPGSPKA
                     QSININKWNKDLKKLAEEFVKKSHKYRFSKQAGSYSLKVGGEVVIKLIQHQQNENYSQ
                     VGNNKKLMLGFSDPNVLLHESDLDETKIQALSIGVGERCKTFHFYPVEENNMDKMIGL
                     VNKYCK"
     gene            complement(4320..4844)
                     /locus_tag="PLF14_05000"
     CDS             complement(4320..4844)
                     /locus_tag="PLF14_05000"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOT70686.1"
                     /translation="MKIVLAYIIEIILYIIIVWLIGYISVLIHETGHAIGFMCATKSR
                     DWRITIGSGRKILESKRFTVNLLPFGGLFKPGYNGQMSIKRNKLSMLAGGPLATLLLL
                     IPLILLRIVKPGETDIVSAGMLKRIIGFASEYNLVLLVVTVLPFSYPFGVNKGMESDG
                     LQIWRQLKRNDRDK"
     gene            complement(4877..5017)
                     /locus_tag="PLF14_05005"
     CDS             complement(4877..5017)
                     /locus_tag="PLF14_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70687.1"
                     /translation="MSKVRIGIFGVRRGSSVVRYCRTQPDAQLVAICDNGRYITSPDS
                     VI"
     gene            complement(5094..6386)
                     /locus_tag="PLF14_05010"
     CDS             complement(5094..6386)
                     /locus_tag="PLF14_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018214459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT70688.1"
                     /translation="MKRKPLQDLIKWKEDRERKPMVLKGARQVGKTWLMKEFGSNYYD
                     NYVYFNFDEEDQLKSIFEANKNPHRIVELLSLIAGKKILPQETLIIFDEIQECSEALN
                     TLKYFKEKANEYHVIAAGSLLGTLLAKPKSYPVGMVNVLNIFPMCFEEFLEATDPMLF
                     TYYESIEKRQEIFDIFHNRLIDAYNYYLIIGGMPECVSSWVKYKDPAGISKIQRELIE
                     IYENDFSKHNGNVNSGRILQVFRSIVAQLSKPNEKFMYGTVRKGGRARDFEEAIEWLV
                     SAGMLNRVYNVSKMKHPLAAFDKLEQFKLFVFDTGLLKHMAGIDNSAILLSSDFQFKG
                     PLTENYILQQLRGQFEVEPRYYSDKNNELDFVLQYGTAIIPVEVKGGTDKSAPSFKKY
                     ISEHQPEYAIRYSKRGYKKDGYITNIPLYLAGKTKELL"
     gene            complement(6744..7868)
                     /locus_tag="PLF14_05015"
     CDS             complement(6744..7868)
                     /locus_tag="PLF14_05015"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOT70689.1"
                     /translation="MILIKGQFNEAKVFTSELEETARAQIQNLVNQSFVKGSKIRVMP
                     DVHAGAGSTIGTTMTIKDKVVPNLVGVDIGCGMLTCELTGITEENLNLQKLDNLIRFR
                     IPSGYDIRRNPHEFLDRTRIDDLRCREKGKNDGKLKMDRARLSLGTLGGGNHFIEINK
                     DENNKLYLVVHSGSRHLGLQVALHYQRLAAKLQPEIERDLAYLEGEPLKDYLHDMKIM
                     QEYAVWNRKAMAETIITGMEWQTGARYTTIHNYIDLNTMILRKGAISADAGEIVLIPL
                     NMRDGSLLCVGKGNPDWNFSAPHGAGRIMSRNEAKRVLSMKDFKDSMDGIFSTSVRKD
                     TLDEAPMAYKSLESIQEYLDQTVDILHHLTPLYNFKATGD"
     gene            complement(7985..9262)
                     /locus_tag="PLF14_05020"
     CDS             complement(7985..9262)
                     /locus_tag="PLF14_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004024482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOT70690.1"
                     /translation="MNKKLYKKRIIDSEVKRYLAAFGAVCIEGPRWCGKTWTSAFHSN
                     SEIYIADPFGGYQNRRLAEMDPSLVLQGEYPRLIDEWQEVPQLWDAVRSTVDKVGKKG
                     MFILTGSSTPKHKGILHSGAGRIARLRMNTMSLYESGNSDGKISLRDICMGTAPSIMT
                     GEVELIQIIDFIVRGGWPENIGIPLEDAALLPLQYLNAIIEDDVYRLDGIKRDMSKMK
                     KLLRSLARNESTTATNNTLKRDIKDVDSEDIDNKTVSEYLDIFNRLFLTNNQPPFSPS
                     MRSSTRIKQMEKRHFCDPSLACALLSATPKKLIGDLNTLGFLFEALVERDLEIYASSF
                     DGKLYHYQDYDNKEIDAVIELPDGTWTAFEIKLGANEIDAAAESLLSVKKAFENDLPE
                     KAPKYLCVVCALSNAAYMREDGVYVVPVTSLRN"
     gene            complement(9675..10265)
                     /locus_tag="PLF14_05025"
     CDS             complement(9675..10265)
                     /locus_tag="PLF14_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70691.1"
                     /translation="MDKIILILIIIALLTVIVICFYRKNSAKSEHPDKSEFSNRGTDY
                     KNPGDLSRGIIYEPDMAKKPLPPFKEITVVDNDDIAIKITAADPYHMWGYSLHVYLEN
                     KSSGDTFYSFALSSAKVNGIRLKTDEVATDVNFYIELGKNYSYASGEIAFIAEDLKKK
                     GINKYGDIKNIELGFIVYETLNKDKVIFTGTVTVKL"
     gene            complement(10334..10963)
                     /locus_tag="PLF14_05030"
     CDS             complement(10334..10963)
                     /locus_tag="PLF14_05030"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009267454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOT70692.1"
                     /translation="MKRCIIIGGAPICDYEYIRERVNKEDFFIYCDSGLKHKEKLGVS
                     PNLIIGDFDSHSNPYLDIETIVLPVMKDDTDTAYAAKEAIRRGFRDIILCGVTGGRQD
                     HTLVNIFLLFKFDSYGIKARIIDDYAEMEVLSPGRTVLIEDCYLYFSLVNMTGIARGI
                     TIKNAKYNLSNAKIESENQYATGNEVLPGKTAEVSIEEGRVLLLKVRRE"
     gene            complement(11257..12483)
                     /locus_tag="PLF14_05035"
     CDS             complement(11257..12483)
                     /locus_tag="PLF14_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70693.1"
                     /translation="MINSRNDVIELLREYTITQREYKWNINRPVAFLNGVVLGKFREN
                     DALTRKTLKKGVTDDALCTEINAYLERHIKNIEELEYPNIMQQMKLESYKKQKNELSN
                     TLIDKWISKNCGWIVECVKEISYPEERVLLYLYYAFDNYNFQRQHPFCGDLDELDKEY
                     KNVFDKHSQYRYGLVPVDGDRELIPINPPRIYDKSLNKTFFTKNVPMNLLEKISEMMS
                     NGLVKDFSVRLLNEPGYEGKLNRVYLLEELERGKVFDFTNLGNYSITRLYSQKYEDCM
                     WVVIDPTNITFEELCDDFKIYNDMIVTQVVHLEYEEKGMHYITHLDHEYVFYTIDEYK
                     NKIMGNVTQKGTAKHRLKSFKIDNSRIPFDHRFKIIRKDENGNDLPEEDEQVLCYVLE
                     CYFRHKDLLKEYFHKV"
     gene            complement(12575..12742)
                     /locus_tag="PLF14_05040"
     CDS             complement(12575..12742)
                     /locus_tag="PLF14_05040"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT70694.1"
                     /translation="MSAKQAAELAGINAKTLYAYEEGTRMIKLDTLYKLSQIYDISID
                     ALMDQAKQIIR"
     gene            complement(12926..13135)
                     /locus_tag="PLF14_05045"
     CDS             complement(12926..13135)
                     /locus_tag="PLF14_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70695.1"
                     /translation="MKRHYLRNNKTDFYKWCRSYVHKALYDVKRFENKNGGLDLILVW
                     GEYCKALEIVRQYLVIKAGLPTDYQ"
     gene            complement(13132..13362)
                     /locus_tag="PLF14_05050"
     CDS             complement(13132..13362)
                     /locus_tag="PLF14_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70696.1"
                     /translation="MGLVEHRNSIWNIDYLKYGEVRNAGLSIYSDALFDEISKAVRYQ
                     YSTIVSFNLLLKDIGKEDALYIDIFQKNDVGK"
     gene            complement(13562..13780)
                     /locus_tag="PLF14_05055"
     CDS             complement(13562..13780)
                     /locus_tag="PLF14_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70697.1"
                     /translation="MNYTKTIRNYCQQNEGHIFDSQQMFRDFFSMVPYKTFIKILNRL
                     EDENILSSVSKGVYLFNPNNPAMQIQLF"
     gene            complement(13856..14207)
                     /gene="ssrA"
                     /locus_tag="PLF14_05060"
     tmRNA           complement(13856..14207)
                     /gene="ssrA"
                     /locus_tag="PLF14_05060"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(14198..14668)
                     /gene="smpB"
                     /locus_tag="PLF14_05065"
     CDS             complement(14198..14668)
                     /gene="smpB"
                     /locus_tag="PLF14_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOT70698.1"
                     /translation="MAKEDIKVISYNRKAGFDYELIEFYEAGIVLTGTEVKSLRAGAV
                     NMRDSYGYIKDYEAYVSGLHISHYDKGNINNVDPDRDRKLLLHKSEIRKLVLKMQQKG
                     YTLIPVKLYFVKNKVKLELALAKGKKLYDKRDSIQKGEVEREIKRRMKDNHTGA"
     gene            complement(14744..16294)
                     /locus_tag="PLF14_05070"
     CDS             complement(14744..16294)
                     /locus_tag="PLF14_05070"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HOT70699.1"
                     /translation="MRKRTFSENFIKPLSLFISIFICLNFLSICNSVTVIAAELSSVH
                     PDHVRGLYVKADIASLGLKEENVERYSEYIEALKEKKNSKIDIGKINAFEKIVGLINA
                     TRLNAVVINVKDDQGYVTIPADSEYVLQAGSYNYQLKDIGKTMEQLKKYGIYTIARIV
                     VFKDGVLAQNASHAIKYDDGTVWKDYSGAAWVDPYDEWVRQYNIEVAKQAAAAGFDEI
                     QFDYIRFPEKFSQYDVSKKYLQNKIRQDQLIRQFLKDAYTQLKPYNVKVSADVFGCAA
                     YLWDDQLNIDIGQVWYNLTQEADYISPMVYPSHYRGTNWYTQGDPNKHPYEVVKGAIE
                     DSLTINSAFEDKARIRFWLQDFSMYDYEYGPQQIADQVKALHEKGIDTYMFWNNKNVY
                     DPYNYLILEDKHPKVIPDKYPVYDIKNNNPADAAKRYLEADKNKDLYHSFILKAEKTR
                     QSEYADYVKNMQWSGITAYEFTGYRESFNTAQVFFSITKQGKQQKAVLFMLLEEGIWK
                     VHDYYIIR"
     gene            16375..17157
                     /locus_tag="PLF14_05075"
     CDS             16375..17157
                     /locus_tag="PLF14_05075"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HOT70700.1"
                     /translation="MHIHIITDLEGISGVTDISQIDYDTDGYEYARIRLMADVNAAVR
                     GAFNGGADTVTVTDGHGRGKNFIDGALDKRAVNINLIELYKQGIHKQVDAVMFVGTHA
                     MPGTIGAFLDHVQSSVSWYNYYLNGIRCGELVQGGAFFGSYGAPVIFASGDEAACSEA
                     KHFFGDIQVAAVKRAIGRNKAEAYDFDLCEKIIESNAAAAISLADKIKPYTVSKPVEI
                     IVDFTRTDYCDRAAERPGVERVDARRVIKRIPEITDYLSVLL"
     gene            17172..18416
                     /locus_tag="PLF14_05080"
     CDS             17172..18416
                     /locus_tag="PLF14_05080"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010277535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOT70701.1"
                     /translation="MKKQLALYGGEKVKQQPYGTGRRFGEEELTQLKEALDQNTLFYW
                     SGNKVKTLCAKFAKMYGVEHCVAASSGTAAIHVALGALGVTEGDEVITSPITDMGSII
                     GIMYQNAIPVFADLDPHTYNMDPKSIEKCITEKTKAIVVVHLAGNPAEMDEIMAIARK
                     HNVFVVEDCAQSFMCRYKGRLAGTIGDMGCFSLNDFKHISAGDGGLVLVNNPDLYVKT
                     FMFADKNYNRFSKGAALRVIEYLAPNYRMNELTAAVGIAQLDKLEKICATRKRYGEMI
                     TEGIKHLKGVYPHEVKKGNQSSFWFYMFRIDPKIVGTDTTTFAEALKAEGIGCQRGYI
                     PSVVYLYDMFQNKEGYQGTHAPFDSKYYGKDISYPKGLCPVAEEILDTAVRLNLNEFY
                     TEQDIQDIIDSIVKVTEYFKKG"
     gene            18431..19294
                     /locus_tag="PLF14_05085"
     CDS             18431..19294
                     /locus_tag="PLF14_05085"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT70702.1"
                     /translation="MFRVGMLGTENSHALAFAKEICFKEEYKDFKITAFYALEKAPSE
                     NIIEKCGITDAVIVDNPLEMMDLVDCVIVTNRHGKYHKSCSLPFIEKGKPAFIDKPFT
                     IDPAEAREILKTAKQKKVPVSGGSGCKYSPQILELKKLVEQETFGRIYSGVLNFPASV
                     ESEYGGLYFYASHLVEMTLAVYGMDIKAISAFLNNGHITAIAHYDKYDITMQFANSKK
                     YVSVIYGETDVDVRYLDMSGIFIAEFKDFVDMVRTGKSPLTDDQLLMPVLVMNAIEKS
                     LEQKKTVRIDR"
     gene            complement(19395..19931)
                     /gene="thpR"
                     /locus_tag="PLF14_05090"
     CDS             complement(19395..19931)
                     /gene="thpR"
                     /locus_tag="PLF14_05090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOT70703.1"
                     /translation="MRTFIAINFHHDVKDRLFNIERSLKSMAERGRFTSKDNLHLTLI
                     FLGEIAEDRVKLAQLCVKNISAPKFDMVVNRLGKFRRPDGDIYWAGLETNPVLNSIYS
                     QLYSNLSSYDFYIKDSEYVPHLTLARDYVLKDKEAMERYKENFEKISIHVESIHLMKS
                     EIIDRKLTYTVLYTKELD"
     gene            complement(20096..20566)
                     /locus_tag="PLF14_05095"
     CDS             complement(20096..20566)
                     /locus_tag="PLF14_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70704.1"
                     /translation="MRKNTAVKFLISVMVVVLALSLAGCGPKARVREEVELFVEKANA
                     LDYQGMFDLMAPETVREIRGNKSVADFLKEKTSAEIADAIVKQLLPAPVGDAEQFLKT
                     LTMEYEGADIREDFSSLYCVVTYEIDGQKFEKDAVFNMLRKNVSEPWTVRDIAL"
     gene            complement(20699..22348)
                     /locus_tag="PLF14_05100"
     CDS             complement(20699..22348)
                     /locus_tag="PLF14_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT70705.1"
                     /translation="MRNINLRYVDESYDEFGVLKSFKLKYPDNFNFGYDVVDDIAIND
                     PHRKALVWCNPEGEERIYTFADIKRLSDKTANYLRSLGIEKGDKVIVILKRHYQFWYT
                     SVALHKLGAIMIPVTFMITAHDAEYRINAASVKAVICTGEGHAAQAIDDVLNICPSLS
                     VRIMAHGHKEGWSDFDKGVQLADEYFERVETHVKEPLLMYFSSGTSGYPKMVLHNHTY
                     SLAHLLTAKHWHNVHPEGLHLTIADTGWGKAVWGKLYGQWLMEAAVFAFDYDKFNAAE
                     ILSLIPKYKITTLCCPPTMFRFFIKEGLEKFDLSSLTYATTAGEALNPDVFETWYKVT
                     GLKLMEGFGQTETILAVCNLVNMQPKPGSIGKPSPLYEIDLLDTDGNVCPTGVTGEIC
                     IKISNRYPDGLMDCYYRNKEMTDCTIYNGYYHTGDTAWKDEDGYLWYVGRNDDIIKSS
                     GYRIGPFEIESVLNSHPKVLESAVTGVPDEIRGQIVKATVVLAKGYSPSPELVKELQD
                     FVKHETAPYKYPRVIEFVRELPKTVNGKIRRAAIRERDRQA"
     gene            complement(22348..22932)
                     /locus_tag="PLF14_05105"
     CDS             complement(22348..22932)
                     /locus_tag="PLF14_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT70706.1"
                     /translation="MELEISEIAQRIRTLREIFDFTVEEMASATNVSVEDYIKLESGK
                     SSDYSFTFLYRCAEKFNVDIVEILTGENPHLGQYAIIRKGKGLPIKRREYFLYSHLAP
                     NFKNKTAEPFLVRAPYNEKEYNEPLKMNKHEGQEFDYILEGALKFQHGDREEMLYAGD
                     SVYYNSGIEHGMTAALGKECVFLAIVMKDVSKER"
     gene            23084..23773
                     /locus_tag="PLF14_05110"
     CDS             23084..23773
                     /locus_tag="PLF14_05110"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOT70707.1"
                     /translation="MKRLKKIIIICGHYGSGKTNFAVNLAVDLRKQGYPVTLTDMDIV
                     NPYFRSSDHSEMLDSKGIKVIAPVYANTNLDAPAIPAEMYSAFDRSGYSLFDMGGDDA
                     GATVMGQFFRNITGKEYDMLYVINGKRAMIENVHDAAEMLYSIESASRLKATYIVNNT
                     HLKDYTDAKTILESMEYAEKVSEAVNLPILCTTYPVSMIKEQEISATGGIITQHLYPV
                     EVFVKTPWEVN"
     gene            23785..23991
                     /locus_tag="PLF14_05115"
     CDS             23785..23991
                     /locus_tag="PLF14_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015760731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOT70708.1"
                     /translation="MAKAIIDETKCKGCGLCVAACPRKILVLSPDKINSKGYRPAQVV
                     DQSKCTGCASCAIMCPDVAITVER"
     gene            23998..25062
                     /locus_tag="PLF14_05120"
     CDS             23998..25062
                     /locus_tag="PLF14_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOT70709.1"
                     /translation="MAKVLMKGNEALAEAAIMAGCRHFFGYPITPQTELAAYMAKKMP
                     KIGGVYLQAESEIAAINMVLGASAAGKRAMTSSSSPGISLKSEGISYIAGSDLPAVIV
                     NVQRGGPGLGGIQPSQADYWQATRATGHGDFHLMVYAPSTVQEMTDLVALAFDNADKY
                     RMPVMILSDGMLGQMMEPVELKTANATDCPIEKPWAACGHRNSRPHNVINSLYLTPQA
                     LEELVKKRYEKYEVIKKEEQMADEYLTDDADIILVAYGASSRICRTAVNTARKEGIKA
                     GLIRPITLWPYPAKYIEKHIAHAKAFLCVEMSMGQMVDDVRLCVNGRKPVAFYGRTGG
                     IIPAPDEVLNEIRKLTGGVK"
     gene            25062..25811
                     /locus_tag="PLF14_05125"
     CDS             25062..25811
                     /locus_tag="PLF14_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015491870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOT70710.1"
                     /translation="MAVVFQKPQALLDVPFTYCPGCTHGIIHRLVAETIDELGIEGHT
                     VGVAPVGCSVMNYNFFACDMVQAPHGRAPAVATGLKRAIPENIVFTYQGDGDLAAIGT
                     AETVHAAVRGENITVVFVNNAIYGMTGGQMAPTSLPNQVTQTTPYGRDTSYSGHPIRV
                     CEMLATLEGTALAQRVSVDNVKNVQNAKKAIKKAFTYQIEKRGFSIVEVLSTCPTNWG
                     LDAQESLQWLRDNMIPYYPLGVYKDVGGTTK"
     gene            25808..26341
                     /locus_tag="PLF14_05130"
     CDS             25808..26341
                     /locus_tag="PLF14_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012803180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOT70711.1"
                     /translation="MSTLNILLAGFGGQGILFLGKMAAYAGLMDNRQITWLPSYGPEM
                     RGGTANCSVCISDDLIGSPLVINPDILVAMNLPSLEKFIDKVVPGGTVLVDCSIIDKK
                     VHREDINVFYVPATRLAADNGLPGLANMIMLGKMLKETKFCTENALEEALLKCVPPRK
                     ADMMELNRKALKIGSEL"
     gene            26341..26889
                     /locus_tag="PLF14_05135"
     CDS             26341..26889
                     /locus_tag="PLF14_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOT70712.1"
                     /translation="MEETMEIARRIAGIRDACGYSQEDMAKELNIDIETYREYEKNGQ
                     NIPISVIFHIANKFRVDLSEILTGTSAKLDTYHMVKAGHGKEVDRYPGYNYTDLAFRY
                     SKKIMQPLLVTLDPSDEPAALVSHTGQEFNYVIKGKMAFSFDDQQIILDEGDCIYFNA
                     THSHGQACASDVPVVFLTVIAE"
     gene            26898..28598
                     /locus_tag="PLF14_05140"
     CDS             26898..28598
                     /locus_tag="PLF14_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT70713.1"
                     /translation="MLEKYLPRTEFASYEDFKQNYKVIVPDNFNFGFDIVDAWAEQDK
                     DMPALVWCDDLQNEMHFTFADIKKLSNRAANYFISLGIKKGSTVLVILRRRWEYWIIA
                     VALHKIGAVLIPASLQLTKKDITYRANAADVQAIICLNDDFVIAQTEGAFPDCPTVKN
                     LILVNRDNNTDRNKGSTVSEIWKDFSEEAFKCSDVFKRPAGKEATKTTDTMLLYFTSG
                     TTGMPKMVHHDYSYPLGHIVTAKYWQCVEKGKLHMSVSDSGWAKFGWGKIYGQWICGA
                     VIFAYDMEKFIPANLLKMMEKYRLTTFCAPPTMFRFMLQEDVKQYDLSSIKRFCIAGE
                     PLNPEIFRKWKEITGKPLTEGFGQSESSVMIANFKWIEPKPGSMGKPAPLYDIQLINA
                     DGIACEDGEEGEITVRNMESEHPVGLFLGYYKDTELTKQHIQNGVYNLKDVAWRDNQG
                     YYWFVGRHDDVIKCSGYRIGPFEVESALLEHEAVVECAVTAVPDQLRGQIVKATVVLK
                     KGYTPSAELIKELQNHVKKVTAPYKYPRIIEFTEELPKTIGGKIKRGEIRNSDKEGSK
                     "
     gene            28595..28822
                     /locus_tag="PLF14_05145"
     CDS             28595..28822
                     /locus_tag="PLF14_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70714.1"
                     /translation="MKVLSKIFIILAILLSNVMCAVVAYNYCSLEWGAKYAGYSAPPG
                     AALALAIPYAAGIVILIVLAIIFNRKAGKKS"
     gene            complement(29143..29805)
                     /locus_tag="PLF14_05150"
     CDS             complement(29143..29805)
                     /locus_tag="PLF14_05150"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT70715.1"
                     /translation="MTISKAWDWNKELNSVWLQPSEESYYVAQLWKDRNIKRVLDFGC
                     GLGRHSIFFAKQGFSVSAFDLSLEATEYLKAWAERENLSIEVINADMMKIPYEDNFFD
                     AIFAYHVISHTDSSGIKEIIKEISRILKIGGEIYVTLCSKESASFKDTDNPKRDANTV
                     IKTKEGPEKGIPHFYVELDDIFSLFDNYGIDRIRHTDDCYFDGKKQNSKHYFISASLR
                     KK"
     gene            complement(30026..31168)
                     /locus_tag="PLF14_05155"
     CDS             complement(30026..31168)
                     /locus_tag="PLF14_05155"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOT70716.1"
                     /translation="MRFYEDKISQKIKTGEIVCGDHTKCHRSVNGTVFALCDGIGSGI
                     YANISAISCSDRIVELFKLGVSLRSTVETVAASMQRARTEDHPFSAFSAVAILPDGQF
                     SIYTYEAPQAIIIKNGTSSVLKPEFFTTGYEVVGETVGVLDIGDSLVLCSDGVTQAGL
                     GHGYGFGIGIENISDYINKLLQSGTDIHDLPEKITDMCARLMNGRYEDDTTLAVLHCQ
                     EANELTVMTGPPSKVSMDKEYVERFISMPGKKAICGSTTADIVARETGRKVETLKMGT
                     AFGIPPEYAIEGIDFTGEGAIILNQVYNILDEPVENFTANSAAERLCMMMLEADVIHM
                     MIGNAMNDAHEVLLFKQVGVRVRRTAIKLIAEKLREKGKLVIETYY"
     gene            complement(31177..32880)
                     /locus_tag="PLF14_05160"
     CDS             complement(31177..32880)
                     /locus_tag="PLF14_05160"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOT70717.1"
                     /translation="MRQNTIYTVTADCQDCYRCVSSCSVKAIKVSGGQAQIQDDLCIK
                     CGTCVRECPQHAKVIRSSINEAKELIASGRKVSVSIAPSFAALFSGKLAARLPSALRQ
                     LGFNMVFETAEGASYITDKSFAKSEKGTICTACPVVVSYIEKYKPELINLLIRVTSPM
                     IAHGRMIKEKYPDNAVVFIGPCAAKKEEALRPENAGVIDVVLTFTEIRKWFEDEDIHL
                     ENCSESDFECIGEIGDSRLFPLEGGMFKTGGIHSDVTAADVLHVSGAEDVMTLLDSPE
                     TLLRYSKIEPLFCKGGCVNGPGFESDKNVFERRLDIIEYTEKAKRKKQTDSIGDIAYF
                     TEFSDAEVNEDTVTEEQIYKVFEHTGKADPKFQLDCGACGYKSCVENAKAVVRGMAEP
                     EMCIPYMRRLAQQRSDRIIETSPNGVVILNKELCIIKMNPAFQKMFMCNNGLLGRRIS
                     YLINADGFEKLLAGTIEHHESIQVKYGIKYHEILYALRDEEQFVGIFSNISKIKFDDS
                     QLDLIKAQTVAHAREFLEHQVKFAQEMAHYLGKSTAQSEEIAQRLIDLYKSDANISDN
                     K"
     gene            complement(32907..34898)
                     /locus_tag="PLF14_05165"
     CDS             complement(32907..34898)
                     /locus_tag="PLF14_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HOT70718.1"
                     /translation="MKTQTAVIDNFTIQIEDEKNLLELIRKANIELPTFCYHSEISVY
                     GACRMCMVEIEGRGIVPACSTKPEGGMVVHTNTAQIRKMRKMIIELMLASHDQSCTTC
                     PKSGDCKLQNIAKQLGVDKIRFKQMKTHQELDLSSDAIIRDPGKCILCGDCVRVCNEI
                     QSVGALDFAYRGAEAKVVTPFNKGIGEVECVNCGQCVKVCPVGALMPKYHINDVWEAI
                     HDKSKTVVAQIAPAVRVSLGEYFGYKPGTTTTGQIIAALRRMGFDKVYDTSFSADLTV
                     IEEGREFLDRYSKNENLPLFTSCCPAWVKYAEQYYPDLLSNLSTCRSPQQMFGAVCKE
                     QLVKDLKISREDLVVVSIMPCTAKKFEARRDEFKTNGNPDVDYVITTKELSIMIKESG
                     IEFGKLELSSFDMPFGFTTGAGVIFGASGGVSEAVLRFAAYSLQKGFVGEFRQFRTGD
                     RTKVSEIEIGDKKLRLAVVSGLANAKKVIDKIRKGEEHYDLVEVMACCDGCINGGGQP
                     VSNASNAVADRAKGLYDNDKMLQFHVSSENPYIKQLYNETLVDDKAHRLLHTTYENRR
                     RILQDDIIINEVPENKKLEITICFGTSCFLRGAQTLYTQLMEYLSERELLGKTEFTAS
                     FCGKQCKKGPVISINGKNIEHCTLEMAVAEIEAVLRLKM"
     gene            complement(34909..36876)
                     /locus_tag="PLF14_05170"
     CDS             complement(34909..36876)
                     /locus_tag="PLF14_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOT70719.1"
                     /translation="MNASMLTKQIIDLEKTRENYNKACTAYKMRIVICAGTGCMANGA
                     MSIYNKFREVIAERGLKADVVLEKEEHHHVAHNDKHAAAGESQTVNNNFLLVQSGCQG
                     FCQMGPLVTIYPQNILYTKVKVSDVEEIVDRTLQKGESVDRLLYECHDTKHHVKDMNK
                     IPFYERQKRIMLKLCGHINVNDVDEYIANGGYFMARKAVLEMTAEQICKEILDSGLRG
                     RGGGGFPTGRKWELTRINESDTKYIICNGDEGDPGAFMDRCVMEGNPHSVLEGMIIAA
                     MAVNASIGMVYVRTEYPLAVQRLKKAIKSARENGILGENIFGSGKKFDIEVIEGAGAF
                     VCGEETALIASIEGHRGMPTMKPPFPAEKGLFGKPTVINNVETLATIPVILEMGAAKY
                     RELGTETSKGTKTFALTGHVANTGLIEVPFGTTLREIVGDIGGGVLDDEGNNCGVDFK
                     AVQIGGPSGGCLTKEHLDLPLDYDNLRNVGAMVGSGGLVVMNQNTCMIQIARFFMQFT
                     QNESCGKCVVCREGTRQMLALLDEIIEGRADAQTLELLEELAETVRIGSLCGLGKTAP
                     NPVLSTLEYFRDEYNAHVFDKRCPTGNCEALKTYRIIAEKCKACGLCAKKCPVEAIEG
                     AKGVPYVIDAEKCIKCGACINACRFNAVVKG"
     gene            complement(36873..37382)
                     /locus_tag="PLF14_05175"
     CDS             complement(36873..37382)
                     /locus_tag="PLF14_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOT70720.1"
                     /translation="MGNDRNTFSIKFDKVCEVLDKHECSPNKLIAILQEIQEEYKYLP
                     EDIMTYIATALGISPGVVFGVATFYSHFALEPKGKYVIKVCDGTACHVRKSKDILQTL
                     EKELGLSGKKKTSDDMLFTLETVACLGACGLAPVVVVNDEVHGSMTKEKTIELISRIR
                     EQERQEMNK"
     gene            complement(37461..38090)
                     /locus_tag="PLF14_05180"
     CDS             complement(37461..38090)
                     /locus_tag="PLF14_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009986123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOT70721.1"
                     /translation="MKQPISLQLYNRLTKYLYYLEGLGADAPENISATTIAKALELND
                     VLVRKDLASVSNGGRPKTGYVTKELMADISHFLGHDNTSDAVLAGVGNLGKALMSYDN
                     FKNYGLDIVAAFDISPELVGTEINGKKIFACEKMKNLCERLNIKIGIITVPAEHAQSV
                     CDMMIECGIKAIWNFAPVNLKLPDDVVVKNENMAASLAVLIKRLDQKEK"
     gene            complement(38357..38530)
                     /locus_tag="PLF14_05185"
     CDS             complement(38357..38530)
                     /locus_tag="PLF14_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70722.1"
                     /translation="MRKKRLSFTILLLICLQMFVLWNNAFACHASDDFVYRRNKSMLF
                     EQAVNTDDYILRK"
     gene            complement(38542..38799)
                     /locus_tag="PLF14_05190"
     CDS             complement(38542..38799)
                     /locus_tag="PLF14_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70723.1"
                     /translation="MRIKVKSDDGKKINLIFPTWFVFSDLGAKIAVKAISRNAEPHEF
                     NLSGKDLSRLMAEVRKIKKKYGRFELVDVQSAEGDIVKIVL"
     gene            complement(38801..39292)
                     /locus_tag="PLF14_05195"
     CDS             complement(38801..39292)
                     /locus_tag="PLF14_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT70724.1"
                     /translation="MRTLGNRIAELRRGKNITQDEMAEKLNVTAQAVSKWENDISCPD
                     IMLLPKIAQMLSVTVDELLSDQPKKSVELVPEEKRKSIDDLVLRIYVNSKDGDKVKVN
                     LPMPLVKAGLEMGLKMPEVSGNEALKDVDFAQLIALADKGVVGKLVEVESVDGDTVDI
                     VVE"
     gene            39602..40090
                     /locus_tag="PLF14_05200"
     CDS             39602..40090
                     /locus_tag="PLF14_05200"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOT70725.1"
                     /translation="MKVILHDLDWQYSQRLLTKCDAIVEADGKYAPCKGCFGCWVKHP
                     AECFIKDKLQTACRILGRADQLVIITKNLYGTYSTKIKNVMDRTIGTSTPLCVYRQRE
                     MHHTLRYGKHALLKVLVYGDITEREKATFIYMVKRNAVNDGYNKSEVLFFHSAAEAER
                     AV"
     gene            40087..40818
                     /locus_tag="PLF14_05205"
     CDS             40087..40818
                     /locus_tag="PLF14_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70726.1"
                     /translation="MKTVLVNCSPKKRFSASAYLAALQSFFLKGKKVTKNLRSKKDYT
                     PVFEQLKDADSLVLCLPLYADGPPSHVSAFLEEAELFFLKNNLHPNLYCIANNGFIEG
                     RQNEALMQVMENFCHRAGINWCGGVGIGGGVMLNITRIVFVIQTAIFFIQYIIGIIQT
                     GLFLNTGLLYAFLQNAGLLLFFNSGVLFFLIRMGSAVNKCSVSGKHYTRILLPSFIFI
                     LIANIFFTLLSLFQGGLFRGWLKKK"
     gene            40968..42191
                     /locus_tag="PLF14_05210"
     CDS             40968..42191
                     /locus_tag="PLF14_05210"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOT70727.1"
                     /translation="MNKFDEKIKKIVLAYSGGLDTSVIIPWLKENYEGCEVIAVCGDV
                     GQGAETEGLEARALATGASKLYIEDLTEEFVYDFIFPTLKAGAVYEKKYLLGTSFARP
                     VIAKRIAEIALKENADAICHGCTGKGNDQVRFELTIKAFAPKMKIIAPWRIWDIKSRN
                     QEIDYAIAHNVPLKVSRETSYSKDKNLWHLSHEGLDLEDPSNEPQYNKEGFLEMCVSP
                     EQAPDKAEYITVDFEKGIPVAVNGNRMSGPDVIKTLNTIGGKHGIGLVDMVENRLVGM
                     KSRGVYETPGGTILYHAHEVLESLTLDRDTSHYKALVAEKFAELVYFGQWYTPLREAL
                     SAFVDKTQENVTGQVKLKLYKGNIINAGTVSDYSLYNMDFATFEEDNVYNQKDAEGFI
                     NLFGLPIKVKALAGG"
     gene            42193..43569
                     /gene="argH"
                     /locus_tag="PLF14_05215"
     CDS             42193..43569
                     /gene="argH"
                     /locus_tag="PLF14_05215"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOT70728.1"
                     /translation="MEKLWKGRLNSDTDKLADKFNSSIDIDHILYKQDITASIAHAEM
                     LGKTGIIPFSDAKKISKCLKEILKETDDGSLDIDRGFEDIHTFVEYELTKRLGDTGKM
                     LHTARSRNDQVATDFKMYVKERISDVIEYIKELLGVLADHASDNIHTVMPGYTHLQRA
                     QPVTLGHYLMAYAMMFYRDLKRYMSFDSMMNTCPLGSGALAGTTYNTDRHMTAAALGF
                     EMPSLNSIDGVSDRDFATDACYANSMVMLHLSRFCEELILWASWEFKFVELSDGFSTG
                     SSVMPQKKNPDIAELIRGKSARTFSDLNTLLVMQKSLPLAYNKDMQEDKEAVFDSFDT
                     VSSCLKVFTAMLKETVFLKDNMKKAANKGFINATDCADYLVKKSLPFRDAYKITGQLV
                     AYCIQNDYTLETLPLEIYKSHSSLFEQDVYKEISLDTCVERRISYGGPSAQSVLLQIE
                     HIREAVNG"
     gene            43562..>44441
                     /gene="argC"
                     /locus_tag="PLF14_05220"
     CDS             43562..>44441
                     /gene="argC"
                     /locus_tag="PLF14_05220"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOT70729.1"
                     /translation="MVKVFIDGSYGTTGLRIHERLATRADIQFITLPEQQRKDVNKRA
                     KALNSADIAFLCLPDRAAIESVSLINNPDTVVIDASTAHRTAPGWTYGFLEMGEDIYD
                     KVKQSKRICVPGCHATGFIALVRPLIENNIVQKNYPFSCTSLTGYSGGGKAMISDYTD
                     QNRDCSLSVPRIYALTQSHKHLKEMKHYSSISDYPLFNPVVCDIYSMMCVTVNISIKG
                     VTIDDICDIFVKKYGNRPLFDISDIKGESYNGYIPSDDFKDKDSIKIVVTGNDERISL
                     IALYDNLGKGASGAAVQ"
BASE COUNT        12738 a         9840 c         8635 g        13228 t
ORIGIN      
        1 aaagtcgtca aaggaggtat agtaggcaat atcctcaccc aactcctttt tcatatccgt
       61 aagaccttgt tcccaactgt cacagaccgc taccagttgc gcatcggact gtgtcctgca
      121 gtactttata acaaccgaac ctcttctcac accgaaaact ccgattttca ccttgctcat
      181 atacacctct ttatattctc cataatgact gcattataca ccgtttatcc acttttcata
      241 gatacgctga tatgtttcat cggggatatc gggattaccc ttggaatatg acggtacaag
      301 ctgatctttt gcaacagcgg gatcggtaca ccatgtgtca ttacgccatt tgtccctttc
      361 tgaaactatc cgaagatttg gaatgctcat aggtattcct ccggctaata cagaccgata
      421 ggcaaacatt ccgggaagga acatatccaa cgcctcatat atatcaatgg tgtccgcttc
      481 ggtatttccc aagattcggt cgacaaaatt gtgcattaca tgataatcgc tgcctccgtg
      541 tccccacttt cgaacaattg aaggctcgtc cgcttcggga caataggtat cgggacggtc
      601 aaaacactca ccctcatatc tgtcaagatt ggtaaaaata cgactatatg ccccgtactg
      661 agcatcttct ctggaactct ccgctcttcc cagtgttccg tagatacaga accagatgga
      721 atttttcgaa caccctccat ggacactctt tattatcccc ccgttctcca tagttattat
      781 ctccatacct gcgctgccgg atttggctcc cattctctcc attcttgcat taaagggtaa
      841 ctcaaaaccc gttacagata ccgggcgaag accggttata tgaataagag gacctattga
      901 atgggtacag taaaaggtgg catacatatt gttacgccaa tggtgctttt cgccacgggt
      961 tattctcggc catatggatt cgcaattatg aatatactcc ccttctccgt attcgaattc
     1021 tcccaattta cccgagcggt acagttttcg catctcgtaa ggtgcggcca taaaacagta
     1081 attctccgca tatgcataga ctttaccggt tttttccgtc gtttccacaa gttgcaccgc
     1141 ctcagccatt gtctgacaag gcaatacctc acttaaaaca tgtaaacctt tctccataca
     1201 acgcattgca tacggtgcat gctcgtttgc atagttggca agaaccaccg catccatgtc
     1261 atggttaata aagtcgtcaa aggaagtata gtaggcaata tcctcaccca actccttttt
     1321 cttgtccgta agaccctgtt cccatttatc acagattgcc accagttgcg catcgggctg
     1381 tgtttggcag tatttcataa caacggaacc tcttctaaca ccgaataccc cgatcttaac
     1441 cttactcata aacacctctt tttccttgta atcaatcata attatatgtt ttatataaac
     1501 tgattaaaca tataaccgaa ttatacaccg tttatccact cttcatagat acgctgatat
     1561 gtttcatcgg ggatatcggg attacccttg gaatatgacg gtacaagctg ctcttttgca
     1621 acagcgggat cggtacacca tgtgtcatta cgccatttgt ccctctctga agctatccga
     1681 agatttggaa tgtccatcgg tattccgccg gctaatacag agcgataggc aaacattccg
     1741 ggaagaaaca tatccaatgc ttcatatata tcaatagtgt cagcatcggt atttcccaag
     1801 attcggtcga caaaattatg cattacatga taatcgctgc ctccgtgtcc ccacttttga
     1861 acaattgaag gttcatccgc tccgggacaa taggtagcgg gacggttaaa acactcaccc
     1921 tcatatctgt caagattggt gaaaatacgg ccgtgtccgc cgtattgagc atcttctctg
     1981 gagctctccg ctctgcccag tgttccgtag atacagaacc agatagagtt tttagaaggc
     2041 cctaaaccgt gaagactctt tattatcccc ccgttctcca tagttataat ctccatacct
     2101 gtgttgcccg atttggctcc cattctctcc attcttgcat taaaaggtaa ctcaaaaccc
     2161 gttaccgata ccggccgaag accggttata tgaataagag gacctatcga atgggtacag
     2221 taaaaggtgg cataaatatt gttacgccaa tgatgctttt cgccgcgggt tattatcggc
     2281 catatggatt cgcaattatg catgtactca ccttctccgt actcaaattc tcccaatttt
     2341 ccggaacggt aaagctttcg catctcgtaa ggtgcggcca taaaacagta attctccgca
     2401 tatgcataga ctttaccggt tttttccacc gtttccacaa gttgcaccgc ctcagccatt
     2461 gtctgacaag gtaatacctc acttaataca tgcagacctc tttccataca acgtattgca
     2521 tacggtgcat gctcgtttgc atagttggca agaaccaccg catccatatc atgcttgata
     2581 aagtcgtcaa aggaggtata gtaagcaata tcctcaccca gctccttttt cttatccata
     2641 agaccctgtt cccatttatc acagattgct accagttgga catcgggctg tgtccggcaa
     2701 tacttcacaa cagaggaacc tcttcttaca ccgaagattc ctatttttac cttgctcata
     2761 tacatgtcac ctcttttttt tatactatga ctgaattata ctacatacac attgatgatg
     2821 aaatgcataa ttgttgtacc gaatatatct accttgtttc ctcctttttt cagccccctt
     2881 aacaacatta aaattgtttt tgcttctctt gcgactatat tttcttgttg catcataagt
     2941 tatataaatg tcacattctt atcgagcaat tcatactctt gaaattattt tgaataatcc
     3001 caagcatgtg ctaaaatgaa ggtaatagct atattacttg cagaaaaatt aataaaggag
     3061 aacacatcat gaaatacccg gacattctgt tttacggtga acgaggaatc ctcaatggaa
     3121 ttttaatgga tatttataga aatagagaat catgtaagaa agttaataag tttttcggag
     3181 caattaaatt attaaaccgc aaggaagtgc cttggaaaaa tattacttca tgcaagtggt
     3241 tagtggaacc ggacttatca gaatttggag ccccggattt gattgcggta ttagaatcag
     3301 acggaaaaaa atatgcactt tttatagaag ctaaacttga taagtataca aagagttcct
     3361 tccaccttcc acagaaagtt agtcctgcaa gtgtaaaaag atattccagt agattaaatg
     3421 tccaattatc tttccgctat cgttttgtgg aagcttttaa agagataccc aaggacaata
     3481 aagggcataa cacccaagaa atcattgaaa cctcccaaca ttgggatgaa aaaatgcgta
     3541 aacttgcaaa attacccgtg ataacagaaa tgaaaaattt tttgaaagga gtagaagagt
     3601 attacttcat tgctcttact aatgattttt cctcagaaaa agtcatcagt gagatagaaa
     3661 aaaagcagaa tattcatcct ccgctctacg atggcagtta tgaagaattt aagaagcacc
     3721 aatcaaagtt tggagtactc acctacagag acttggaaga cattatagtt ggcaagtgta
     3781 atggaaataa taatgaagaa ctaagctctt tctgtaaagc atgtaaaatg atggatatga
     3841 aaattgcaaa tgattgtaat gaactacaaa ccaatacacc aggaagtccc aaagctcaaa
     3901 gtataaatat taacaaatgg aataaagatt taaaaaaatt agctgaagaa ttcgtaaaaa
     3961 agagccataa gtataggttc agtaaacagg caggttcata cagtttaaaa gtgggcgggg
     4021 aagttgttat taaacttatt cagcatcaac aaaatgaaaa ttattctcaa gtcggcaata
     4081 ataaaaaact gatgttgggt ttttcagacc ctaatgtttt actacatgag agcgacctgg
     4141 atgagacaaa aatacaggct ttatctattg gtgtaggaga gagatgtaaa accttccatt
     4201 tctatcccgt cgaggaaaat aacatggaca agatgattgg gttagtgaat aaatactgca
     4261 aataaaggaa agcattccct aaccaacaga tgttcaataa gctattccaa gagagacaat
     4321 cacttatccc ggtcgttacg cttaagttgt cgccatatct gcaaaccgtc gctctccatt
     4381 cctttgttta caccgaacgg ataagaaaac ggcagcaccg ttacaaccaa caaaacgaga
     4441 ttatattcag aagcaaaacc tataattctc ttcagcatac ccgcacttac tatgtctgtt
     4501 tcacctggtt ttacaatacg cagaagtatt agaggaatca ataatagaag tgttgccaaa
     4561 ggtcctcccg ccagcatgga aagtttattc ctttttatac tcatttgtcc gttatatccc
     4621 ggcttaaaaa gtcccccgaa cggcagcagg ttgactgtaa accgtttgga ctccagtatt
     4681 tttcttccgg aacctatagt aatccgccaa tccctgcttt tggttgcaca cataaatcct
     4741 atcgcatgtc ccgtttcgtg aattaaaact gaaatgtaac ctataagcca cactatgatt
     4801 atgtaaagaa ttatttcaat aatataagcc aatactattt tcataatatt atctttaatc
     4861 tcctgtagct ttgaagttat ataacggagt caggtgatgt aatatatcta ccgttatcac
     4921 agattgccac cagttgcgca tcgggctgtg tccggcagta tctcacaaca gaggaacctc
     4981 ttcttacacc gaagattcct attcttacct tactcatata catgtcacct ctttttttac
     5041 actatgcttg aattatacta cataaaatat atataaaacg caaatgtact gaatcagagc
     5101 aattctttcg tttttcctgc aaggtataac ggtatattgg ttatataacc gtccttttta
     5161 tatccgcgct tggaataacg gatagcatat tcaggttgat gctcggaaat atatttcttg
     5221 aatgaaggtg cggatttatc cgttccgcct tttacttcaa ccggaataat tgcagtgcca
     5281 tactgtagga caaaatccaa ctcattattc ttatcggagt agtatctggg ttctacctca
     5341 aattgaccgc gaagttgctg taaaatatag ttttcggtca gcggtccttt aaactgaaaa
     5401 tcgctgctta aaagaatggc actgttatca atacctgcca tgtgtttaag caatcccgta
     5461 tcaaagacaa acagcttaaa ctgctccagt ttatcaaatg cagccaaagg atgtttcatt
     5521 tttgaaacat tgtaaacccg gttgagcata cctgccgaaa ccagccattc aatagcttct
     5581 tcaaagtccc gcgccctacc cccttttctg acagttccat acataaactt ttcgttgggt
     5641 ttggaaagtt gtgccacaat actacggaaa acctgcagta tacgcccgct gtttacatta
     5701 ccgttgtgtt tagaaaagtc attttcataa atctctatca actctcgctg tatctttgat
     5761 attccggcag gatccttgta tttaacccac gatgagacac attccggcat tccccctata
     5821 atgaggtaat aattataagc atctattaag cgattatgga atatatcaaa aatctcttgt
     5881 ctcttctcaa tactttcgta gtatgtaaaa agcataggat cggttgcttc aagaaattct
     5941 tcaaaacaca tagggaaaat attgagtaca ttgaccatac cgaccggata tgatttcggt
     6001 ttagcaagaa gagtacccaa aagacttccc gcagcaataa cgtgatattc atttgctttc
     6061 tccttgaagt acttaagtgt attcaaagcc tccgagcatt cctgaatttc gtcaaaaata
     6121 atcagtgttt cttgcgggag tattttttta cccgcaatca gggaaagaag ctcaacaata
     6181 cgatggggat ttttatttgc ctcaaaaata gatttcaatt gatcttcttc atcaaagttg
     6241 aaataaacat agttatcgta gtagttactt ccgaactcct tcataagcca tgtcttgccg
     6301 acctgtcgtg caccctttaa caccataggc ttacgctctc tgtcttcttt ccattttatc
     6361 aggtcctgaa gaggtttacg tttcaataaa atcaccatcc tttcagagta gcaattagac
     6421 tttaacacat ttttccggct ttttcaacgc gtttctgcac atttttctga ctttttaggc
     6481 cgcattttac acatttttct gaattactgc tatcgaaaca tagtctatcg gctcagtttt
     6541 ttaatcagat tcaagtcgaa agctgataat gcaacaacat aatcttcata taatgttaag
     6601 aattacatcc gtttccttat attctcatat ttacccattt ttattttttg taagcagttc
     6661 ccttttttat acttatggaa aattacactt atagtaaaaa atatacgtca actattttgt
     6721 cgtatatcaa aactgatatc ggtttaatct cctgtagctt tgaagttata taacggagtc
     6781 aggtgatgta atatatccac cgtctgatcc agatactcct gtatgctctc caaagatttg
     6841 tatgccatcg gtgcttcatc cagagtatct ttacgaacgg atgtggagaa aattccatcc
     6901 atactgtctt taaagtcttt catcgacagt actctcttgg cttcattgcg cgacattatt
     6961 ctgcctgcgc cgtgaggagc ggaaaagttc cagtcgggat ttccttttcc tacacaaagc
     7021 agagagccgt cacgcatatt gagcggaata agcacaatct cgcccgcatc agctgatatg
     7081 gcaccttttc gtaaaatcat tgtattaagg tctatataat tgtgaatggt tgtataccgt
     7141 gcacctgttt gccattccat tcccgtgatg atggtctccg ccattgcctt tctgttccag
     7201 actgcatact cctgcattat tttcatatca tgcagatagt ctttcaaagg ttcaccttcc
     7261 agatatgcca aatcccgctc tatttcaggt tgcaacttag ctgccagacg ttggtagtgc
     7321 aatgctacct gcagtcccag atgccgtgaa ccggaatgaa caacaaggta tagtttattg
     7381 ttttcatctt tgtttatttc gataaaatga ttgcctccgc ccaatgtacc cagtgaaaga
     7441 cgtgcgcggt ccattttcag tttaccgtca ttcttccctt tttcacgaca gcgtaagtca
     7501 tcaatccttg tcctgtccag aaactcatga ggattacggc ggatatcata gccggaggga
     7561 attctgaaac ggataaggtt gtccagcttc tgtagattga gattctcctc cgttataccg
     7621 gtcaactcgc atgtcagcat accgcaacct atatccacac ccaccagatt aggtactact
     7681 ttatctttaa tagtcattgt agtaccgatg gtgctgcccg caccggcatg cacatcgggc
     7741 ataacccgaa ttttacttcc tttgacaaat gactgattta ccagattctg aatctgggct
     7801 cttgcagtct cttccagttc ggaagtgaaa actttagcct cattgaattg acctttaatt
     7861 aatatcaaaa tatattaccg tccttgcctt tattatataa aagaagaaag acggtagcaa
     7921 ggaaggcaaa ccgtctataa aaatatactg cttgacgaaa tcaaatctgc actcaaacaa
     7981 aaagctaatt ccttaaagac gtaacaggta ctacatagac accgtcttca cgcatatatg
     8041 ctgcatttga cagagcacaa acaacacata aatattttgg cgctttttcc ggcaagtcat
     8101 tttcaaaagc ctttttgacg gaaagtaagg attcagcggc ggcatctatt tcgtttgctc
     8161 caagttttat ttcaaacgca gtccaagttc cgtccggaag ttcaataacg gcatctatct
     8221 ctttattatc ataatcctga tagtggtata gcttaccgtc aaaagaagag gcataaattt
     8281 ccaaatctct ttcaacaagt gcttcaaaaa gaaaacccag tgtattcaag tcgcctatca
     8341 gtttcttcgg cgtggccgag agtagggcac atgccagtga agggtcgcaa aaatgtcttt
     8401 tttccatttg ctttatcctt gtcgatgatc tcattgaagg tgaaaacgga ggctggttat
     8461 ttgttagaaa taacctgtta aaaatatcca gatattccga tacggtttta ttatcgatat
     8521 cctccgagtc tacatctttt atgtctcttt taagagtgtt gtttgttgcg gtcgtgcttt
     8581 cattacgtgc aagcgaacgg agaagtttct tcattttact catatctctt tttattccgt
     8641 caagcctgta aacatcgtct tcgattatgg cattgaggta ttggagtggc aggagtgccg
     8701 catcttccaa aggtatacct atattttcag gccaaccgcc tcgcacaata aaatcgatta
     8761 tttgtattaa ttccacctcg cccgtcatga tagacggtgc tgtgcccata caaatatccc
     8821 gaagtgaaat ttttccgtcg gaatttcccg actcgtaaag cgacattgta ttcatacgca
     8881 agcgtgcaat tcttccggca ccgctatgta gaattccttt gtgcttaggc gtagaagatc
     8941 cggttaatat aaacatacct tttttcccga ccttatctac agttgagcgg actgcatccc
     9001 acagttgcgg aacttcctgc cattcgtcta tcaatctggg gtactctcct tgtaaaacca
     9061 aagacgggtc catttcggca agtcttctgt tttgatagcc tccgaacgga tcagcaatgt
     9121 aaatttcact atttgagtga aaagctgaag tccaggtctt tccgcaccat ctcggtcctt
     9181 cgatacaaac tgcaccgaat gcggcaagat atctttttac ctcggaatca attattcttt
     9241 ttttatacaa tttcttgttc atttaactgt ccctctgtgt atataagcag aatatcacat
     9301 tccctcacat ttggcaacac caatccctca catttggtaa tttcaatccc tcatatttgg
     9361 atataccaat cctgtatttc tatttgtaat ttaaattaat atcaaaacat atatcagaat
     9421 ccctgttttt attgcataaa ccggtattag cgagcggtct tcaaaaactt cctgtcatac
     9481 ttggaagttg cttgataaaa cgaaggggtt ttgtccaaga ctttatctaa agatagtgct
     9541 tcacgataat ccgattgaaa ttgcactaac tctttcaatg tgtaaaattt ataaatcggt
     9601 agcggtcggg tagtataacc gcctgtcaac aacagacagg taactgcaaa aacccattgc
     9661 aattaattac ctctctaaag ttttaccgtt accgtaccgg taaaaataac cttgtcttta
     9721 ttcagcgttt catagactat aaagcctaac tcaatattct ttatatctcc atacttattt
     9781 attccctttt tcttaagatc ctcggctata aatgcgattt cgccgcttgc ataactgtag
     9841 ttcttgccaa gctctatgta gaagttgaca tcggttgcga cttcatcagt ttttaatcgg
     9901 atcccgttga cctttgcact tgataaagca aatgagtaga aggtatcgcc gctcgattta
     9961 ttctccagat acacatgtaa ggaatatccc cacatatgat agggatccgc agctgtgatt
    10021 ttaatagcga tatcatcatt gtcaactacc gtgatttcct taaaaggcgg cagcggcttt
    10081 tttgccatat ccggttcata gattataccg cgtgataaat caccgggatt tttgtaatcg
    10141 gtacctctgt tgctgaattc cgatttgtcg ggatgctcgg atttggcaga attcttcctg
    10201 taaaagcaaa tcacaattac agtcagtaaa gctattataa tcaaaattaa tattatctta
    10261 tccatctgtt cctcctataa taaatcggta tcagatattc aaacaaacct gcaagcaatc
    10321 cgttccaatt ctgttattct cttctcacct tcaaaagtaa cactctcccc tcctctatgg
    10381 acacctccgc tgtcttcccg ggtagcactt cattaccggt agcatactga ttttcgcttt
    10441 caatctttgc attgcttaaa ttatatttgg cattttttat cgtgatacca cgtgcgattc
    10501 cggtcatatt tacgagagaa aaatacagat agcaatcctc aatcaatacc gtgcgtcccg
    10561 gtgaaagcac ttccatttcg gcataatcgt ctataatacg ggctttgata ccgtaactgt
    10621 cgaatttgaa cagcaggaat atattcacaa gggtatggtc ttgacgcccg cctgtgacac
    10681 cgcaaaggat aatatcccga aaacctcttc tgattgcttc tttggccgca taagccgtat
    10741 cggtgtcatc cttcattacg ggcaaaacaa tggtctcgat atccagatac ggattgctgt
    10801 gagaatcaaa atctccgatt atcagattcg gtgatacgcc gagtttttcc ttatgtttca
    10861 atccgctgtc gcagtagatg aaaaaatctt ctttattcac ccgttccctt atatattcat
    10921 aatcgcaaat cggtgcgccg ccgatgataa tacatctttt catttgaaca cctcatctta
    10981 cggactgttt ttgctatcca aatcatatca tccgagggca ataaaaacaa ttatatactc
    11041 tacagtagtc cggtaataca tcgcaaaaat ctgcattcct ttaggaaggc tatatgaaca
    11101 atctgcttgt cttgaagaga aatcatatat tgatagaact ttagcttatg ggtgcaaatc
    11161 aatacctgtg ggtgcaaatg ccgctaagcg ttggaaatta agcattatta ctttggaaaa
    11221 cacaaaaccg acattaacag atgtctgttt tcccacttaa actttatgaa aatactcctt
    11281 taataaatcc ttgtgtctaa aatagcattc caaaacataa cataaaactt gttcatcctc
    11341 ctctggtaaa tcatttccat tttcatcttt tctaattatt ttaaatctat ggtcaaaagg
    11401 tattcttgaa ttatcaattt tgaaggactt taatctatgt tttgctgtgc ctttttgtgt
    11461 tacattaccc attattttat ttttgtattc atcaatcgtg taaaaaacat attcatgatc
    11521 caaatgcgta atgtaatgca tacccttctc ctcatactct aaatgaacta cctgtgtcac
    11581 tatcatatcg ttataaatct taaagtcatc acacaattct tcaaaagtta tatttgttgg
    11641 gtcaattact acccacatac aatcttcata tttttgcgaa tataatctag ttatagaata
    11701 atttccaaga ttggtgaaat caaatacttt tcctcgttcc aattcttcta ataaatacac
    11761 acgattcaac tttccttcat accctggttc attgagtagt ctcactgaaa aatcttttac
    11821 taatccattc gacatcatct ctgaaatttt ttccagtaga ttcatcggaa cattctttgt
    11881 aaaaaaggtt ttgttaagtg atttatcata aatccgcggt ggattaatag gaatcaattc
    11941 cctatctcca tcaactggaa ctagaccata tctatattga ctatgtttat caaaaacatt
    12001 tttatattct ttatctagct cgtccaaatc accacaaaac ggatgttgcc tttggaaatt
    12061 atagttatca aaagcataat acaagtacag taataccctt tcctctgggt aagagatttc
    12121 ttttacacat tctactatcc acccacagtt tttactaatc cacttatcaa tcaaagtatt
    12181 ggataattca tttttttgct ttttataaga ttcaagcttc atttgctgca taatattagg
    12241 atattccagt tcttcaatat tcttaatatg gcgttctaaa taagcattaa tttcagtaca
    12301 taatgcatca tcagttacac cctttttcaa agtttttctg gttaaagcat cattttctcg
    12361 gaactttccc aaaacgactc catttaaaaa ggcaactgga cgatttatat tccatttata
    12421 ctcacgctga gtgattgtgt attcacgcag caactctatt acatcatttc tcgaattaat
    12481 catttcactt tacctttttt acacagtttt tattatacaa cttttaattt atagtttgcc
    12541 atataatgat cttttcacat ttattcttgt tgttttatct gattatttgt tttgcttggt
    12601 ccattaacgc gtcaatactt atatcatata tctgcgataa cttatacagt gtatccagct
    12661 ttatcatccg tgtgccttct tcgtaagcgt acagagtttt ggcatttata cctgcaagtt
    12721 ccgctgcctg ttttgcgctg atactgttct agattctttc cgctctcaaa agatgtccaa
    12781 ccaaaaaaga atcgatggtt ttgatttttg gctttttctg tggaaaacat acttcctgcc
    12841 aggtttggta gatttctttg ataatgatgg atgcttcttt gctgtgcgca aatgcttttc
    12901 tatgaattaa ataattatcc agaaactatt gataatccgt aggaagtccg gctttaatga
    12961 ccaaatactg gcggactatc tccaacgctt tgcagtactc accccaaacc agtatcaagt
    13021 ctaatccacc gtttttattt tcaaagcgct taacatcata taaagcctta tgaacataag
    13081 aacgacacca tttgtaaaaa tcggttttat tgttcctgag gtagtgtcgt ttcatttccc
    13141 gacatcattc ttctgaaaga tgtcaatgta aagagcgtcc tcttttccga tatctttcaa
    13201 taacagatta aaagaaacaa tagtggaata ctggtatctc acagccttgc tgatttcatc
    13261 aaaaagtgcg tctgaataga tactcaatcc ggcattgcga acttctccat attttagata
    13321 atcgatattc cagatactgt ttctgtgctc aactaatccc aaggtgtgaa tgaggctttt
    13381 taccttctct gaaaaaagaa tgtcaattcc ggttaagtgg tacttattaa tgttcttgtg
    13441 gctaccttgc ggaataacct ttgtgtatat ctcaacggta tcatcctggt aatcactgat
    13501 agaataaacg ttgacatggc atagccgaca agcattccgc gataatcctc tgcatattgt
    13561 tttaaaatag ctgaatctgc atcgccggat tgtttggatt gaataaatag acacctttgg
    13621 aaaccgagga cagtatgttt tcgtcttcca gtcggtttaa tatctttatg aaggtcttgt
    13681 atgggaccat agaaaagaaa tctctgaaca tctgctgaga atcgaaaata tgtccctcat
    13741 tttgctgaca atagttgcga atcgtttttg tatagttcat atgaatccct ctaaaagcca
    13801 tgacaatata aaagtcgaca ccttgtgttt ctacaaaatg tcgacttttc ttgtctggtg
    13861 gaggcggagg gaatcgaacc ctcgtccgaa acaatgtccg taccgacttc tacatgcgta
    13921 gcttactgtt taatttccct ttaaagtccc ctgtaagccg ggtgctttat cggtagcttc
    13981 atagtttacc ctttaacctc aaagctttga ttatcgggtt ttcacggtag atgacacccc
    14041 gatccggacc ccgtgatttc cggtgggatg tttagccagc ttaagctgct aaaggtgcaa
    14101 aattaggttt tgcgtttatt ttaatctctc gttttttaaa gaggccaacg agaatcctct
    14161 gcacgccgtc aatatttcaa tcatcccgtc gaaaccttta cgcccccgta tgattgtcct
    14221 tcattcttct ttttatttcc ctttcgactt cgcccttctg gatggaatct ctcttgtcat
    14281 agagtttctt tcctttagcc aaagccagtt ccagttttac cttgttcttc acaaagtaca
    14341 gctttacggg aataagggta taccctttct gctgcatctt cagaaccagt ttccttatct
    14401 cgctcttgtg caacagcaac tttctgtcac gatcggggtc aacattgttt atgtttccct
    14461 tatcgtaatg ggagatgtgc agccccgata catatgcttc gtaatccttt atgtacccgt
    14521 aagaatcacg catatttacc gctccagctc ttaaagactt cacttcggta ccggtaagca
    14581 cgattcccgc ctcgtaaaac tcaataagtt catagtcaaa accggctttc ctgttatacg
    14641 aaatgacttt tatgtcttct ttagccaagt ccaccctcct tcggaagcaa attataccat
    14701 ataattgaat ttattgttaa ttgttacaaa ttcttaatat tctttaccgt ataatgtaat
    14761 aatcgtgcac tttccagata ccttcttcca agagcataaa cagtactgct ttctgctgtt
    14821 tgccctgctt tgttatgctg aaaaacacct gtgcagtatt gaagctttct ctgtaaccgg
    14881 taaactcata tgcggtaatt cccgaccact gcatgttttt aacatagtcg gcatactcgc
    14941 tctgccgggt tttttccgct tttaagataa aggagtgata caagtcttta ttcttatcgg
    15001 cttccaggta tctttttgcc gcatcggcag gattgttgtt ttttatatcg taaacgggat
    15061 atttgtccgg gattacttta ggatgtttat cttcaagtat aaggtaatta tacgggtcat
    15121 acacattctt gttattccag aacatatatg tatctatccc cttctcgtgc aaagctttga
    15181 cctggtctgc aatctgctgt ggcccgtact cataatcata catggaaaaa tcctgtaacc
    15241 agaaccttat tctcgccttg tcttcaaacg ccgaattgat tgtcaaagag tcttctatcg
    15301 caccttttac cacttcatag ggatgtttgt tggggtcgcc ttgcgtatac cagtttgtac
    15361 ctctgtaatg gctgggatat accatagggc ttatataatc cgcctcttgg gtaaggttgt
    15421 accacacctg cccgatatct atattcagtt ggtcatccca aagatatgcc gcacacccga
    15481 acacatcggc ggatactttc acgttatacg gcttaagctg cgtatatgca tcttttaaga
    15541 actgccgtat cagctgatcc tgccgtattt tattctgtag atactttttc gacacatcgt
    15601 actgtgaaaa cttttccgga aaccggatat agtcaaactg tatctcgtca aacccggccg
    15661 ctgctgcctg ttttgccacc tctatgttat actgccgcac ccattcatca tacgggtcca
    15721 cccaggcggc accggaatag tctttccaga ccgttccgtc atcatacttt attgcatgag
    15781 aagcattctg tgccagaaca ccgtctttga acaccacaat cctggctatt gtatatatac
    15841 cgtacttttt cagctgttcc atggtcttac ctatatcttt tagctgatag ttatagcttc
    15901 ccgcctgtaa tacatattcc gaatcggcag gtatggtgac atagccttgg tcgtctttaa
    15961 cattgataac cacggcattt aaccttgttg cattaataag gcctactatc ttttcaaatg
    16021 catttatctt accgatatca atctttgaat ttttcttttc tttcagtgct tctatatatt
    16081 cagagtatct ttcaacattt tcttctttca gtcccaaaga tgctatatcc gcttttacat
    16141 aaagtcctcg cacatgatcg ggatgcacgg agcttaattc cgcagctatt accgtaaccg
    16201 agttgcatat gcttaagaag tttaagcata taaatatgga tatgaacaat gaaagcggct
    16261 ttatgaagtt ttccgaaaat gtcctcttac gcatacatac attataccga tatgtttagt
    16321 gtttatgaac atattcctgt ggtataatac cttaagaagg gatttaaggt aaatatgcac
    16381 atacatatca ttacggattt ggaaggtata agcggagtaa cggatataag ccaaattgat
    16441 tatgataccg acggttacga gtatgccagg ataagactta tggcggatgt caatgccgca
    16501 gtaagaggag ccttcaacgg aggagcggat accgtaacgg ttaccgacgg tcacggaaga
    16561 ggcaaaaact tcattgacgg agcattggat aaaagagctg taaatataaa tctgattgaa
    16621 ctgtataaac aaggtattca caaacaggtg gatgccgtta tgtttgtggg aactcatgcc
    16681 atgcccggaa caatcggtgc gtttttggac catgttcagt cttccgtaag ctggtacaac
    16741 tactatttaa acggtatacg atgcggagaa cttgtacagg gcggagcttt tttcggctca
    16801 tacggcgcac cggtaatatt cgcatcggga gatgaggcgg catgcagtga agccaaacac
    16861 ttcttcggag acatacaggt tgcagcggtt aaaagagcta ttgggagaaa taaagcggaa
    16921 gcatatgact ttgatttatg cgaaaagatt attgaatcaa atgcggcagc cgcaatatcg
    16981 cttgcggata agataaagcc atataccgtc tccaagccgg tggaaataat agtggatttc
    17041 acaaggaccg attattgtga cagagctgcc gagagaccgg gtgtggagag agtagacgca
    17101 agaagagtta taaagagaat accggaaata accgattatt tatcagtatt actataggag
    17161 gaaaaaactg aatgaaaaag caacttgcct tatacggcgg cgagaaagta aaacagcaac
    17221 cttacggtac gggaagaaga ttcggggaag aagaacttac ccagctcaaa gaagcactgg
    17281 atcagaacac tttgttctac tggtcgggaa acaaagtcaa gacattgtgc gccaagttcg
    17341 ctaagatgta cggagtggaa cactgtgtgg ccgcatcgtc gggtaccgca gctatccatg
    17401 tagctttagg cgctttgggt gtaacggaag gagacgaggt cataacctcc cctatcaccg
    17461 acatgggaag cataatagga attatgtatc agaatgccat tcccgtgttt gcagacctgg
    17521 atccgcatac atacaatatg gatcccaaga gcatagagaa atgcattacc gaaaaaacca
    17581 aagccattgt agtggtgcat cttgcgggaa atccggctga aatggatgaa ataatggcaa
    17641 ttgcaagaaa gcacaatgtt tttgtggtgg aagactgtgc tcagagcttt atgtgcagat
    17701 acaaaggacg tctggcggga accataggtg atatgggctg cttcagtctg aacgatttca
    17761 agcacatctc cgcgggagac ggaggattgg ttttagttaa caatcccgac ctttatgtta
    17821 aaacattcat gtttgccgat aagaactaca acagattttc caaaggcgcc gccttaagag
    17881 taatagaata tctggctccc aactacagga tgaacgagct taccgccgca gtgggaatag
    17941 cacaattgga taaactggaa aaaatatgtg ccacaagaaa acgctacggc gaaatgataa
    18001 ccgagggaat taaacactta aaaggcgttt atcctcatga agtgaaaaaa ggcaaccagt
    18061 ccagtttctg gttctatatg ttcagaatag atcccaaaat agtaggcaca gatacaacga
    18121 catttgccga agctttaaag gccgagggaa taggatgcca gagaggatat ataccttccg
    18181 tggtatatct ttatgatatg tttcagaata aggaaggata tcagggcacc catgctccgt
    18241 ttgattccaa gtattacgga aaagacatat cctatcccaa aggattgtgt ccggttgcgg
    18301 aagaaatact ggataccgcg gtacgtctca atctcaatga gttctatacc gaacaggata
    18361 tacaggatat aattgattcc atagttaaag ttacggaata tttcaagaaa ggttaacagg
    18421 aggtcacaaa atgttcagag taggaatgct gggaacggaa aactcacatg cacttgcttt
    18481 tgcaaaagag atatgtttca aggaagaata caaggatttt aagataacgg ctttttatgc
    18541 gctggaaaaa gcaccgtcgg agaatataat agaaaaatgc ggtattaccg atgcggtaat
    18601 agtggataat cctttggaaa tgatggacct ggtggattgt gtcatagtga ccaacaggca
    18661 cggtaaatac cataaatcct gttcactgcc gtttatagaa aaaggaaagc ccgcatttat
    18721 agacaagccg tttacaatag atccggcgga agccagagaa atattaaaga cggcaaaaca
    18781 aaagaaagtg cccgtcagcg gcggttcggg atgcaaatac tcaccgcaaa tactggaact
    18841 taagaaactg gtggagcaag agactttcgg aagaatatat tccggagtgc tcaacttccc
    18901 cgcttcggtc gagagcgaat acggcggact ttatttctac gcatctcatc ttgtggaaat
    18961 gacactggcg gtttacggaa tggatataaa agcgatatcc gcattcttaa acaacgggca
    19021 tataacggca attgctcatt atgacaagta tgatataacc atgcagtttg ccaattccaa
    19081 aaaatacgtt tcggtcatat acggagaaac cgatgtggat gtacgatatc tggatatgtc
    19141 gggaatattc attgcggaat tcaaagactt tgtggatatg gtcagaacag gtaaatcgcc
    19201 tcttaccgat gaccaattgc tgatgccggt attggtgatg aatgccatag aaaaatcgct
    19261 ggagcagaag aaaaccgtaa gaattgacag ataaatatta attcataact taaaaggagc
    19321 atcttgtatg atgctctttt tatgtgcttt ccggaaaaat ccttaacggt tttgttgttt
    19381 acattatatt ctgcttaatc cagttccttt gtataaagaa cggtataggt aagctttctg
    19441 tctataatct ccgatttcat gagatgaatg ctttccacat gaatacttat cttttcgaaa
    19501 ttctctttat atctttccat cgcttcttta tctttcagca cataatctct ggcaagtgta
    19561 agatgaggaa catattcact gtctttgatg taaaagtcat aggaacttaa gttgctgtag
    19621 agttgactgt atatggaatt gagaacggga ttggtctcca gccccgccca gtatatatct
    19681 ccgtccggtc ttctgaattt ccccaacctg ttgacaacca tgtcaaactt gggcgccgat
    19741 atgttcttta cacacagctg cgcaagcttt accctgtctt ctgctatctc gcctaaaaag
    19801 ataagtgtca gatgcagatt gtctttggaa gtaaacctgc ctctttccgc catgctcttc
    19861 aaacttctct ctatgttaaa caaacggtcc tttacatcat gatggaaatt aatcgctata
    19921 aatgttctca tatctgttct ccctgaatat cagtatatca aatgatgagt attctttcca
    19981 tataaagagg aaagcattat actttcctcc tgttattgtc tctcttaaaa aacccgtccg
    20041 taccttttcg gtaaagacta ttcagttata ttcctctaca aggaatcggt ccgaattaaa
    20101 gagctatgtc tctcactgtc cagggttccg aaacattctt cctgagcata ttgaatacgg
    20161 cgtctttttc aaatttctgt ccgtctatct catatgtcac aacacaatac aatgaagaaa
    20221 aatcctctct tatatcagca ccttcatact ccatggtaag tgtctttaag aactgctcgg
    20281 catcacctac cggtgcagga agaagctgtt tcactatagc atccgcaatt tccgcagatg
    20341 tcttctcttt aagaaaatcc gcaacggatt tattccctct tatttctctt acggtttccg
    20401 gtgccataag atcaaacata ccctgataat ccagtgcatt tgctttctca acgaaaagtt
    20461 caacctcttc tcttactctg gctttgggtc cgcaaccggc taatgaaagt gccagaacta
    20521 caaccattac cgatatcaaa aatttaactg ctgtgttttt tctcatttcc aactcctcaa
    20581 ttcaatttga attcattatt aaataactcg tataataata tattaaaaaa atcttctaaa
    20641 caatagtata acactaaata gtatattttt cacataaaat atcatatttt tccattcctc
    20701 aagcttgtct atcccgttct cttatcgccg ctcttcggat tttcccgtta accgttttgg
    20761 gaagttcccg gacaaattca ataactctgg gatatttata cggtgcggtt tcatgcttga
    20821 caaagtcctg cagctctttt accagttccg gagaaggact gtagcccttt gccaacacta
    20881 ccgttgcttt tacaatctgc cctcttatct catcgggcac tccggtaacc gcactttcca
    20941 gtactttggg atggctgtta agtacgctct ctatctcgaa cggacctatt ctgtaaccgg
    21001 acgactttat aatatcgtcg ttccttccca cataccataa atagccgtcc tcatccttcc
    21061 atgcggtatc gccggtatgg tagtacccgt tatagatagt acagtcggtc atttccttat
    21121 tgcggtagta acagtccatc aacccgtcgg gataacggtt tgatatcttt atacatatct
    21181 cacccgtaac acctgtggga catacattgc cgtcggtatc cagaaggtct atctcgtaca
    21241 gaggtgaagg tttgcctatg gatcccggtt tgggttgcat attgacaagg ttgcatacgg
    21301 ccagtatcgt ttcggtctgt ccgaatcctt ccataagttt aaggccggtt accttgtacc
    21361 aggtttcaaa cacatcggga ttcaatgcct cacccgccgt ggtggcataa gtaagcgagg
    21421 aaaggtcgaa tttttccaac ccttctttta tgaaaaacct gaacattgta ggcggacagc
    21481 aaagtgtagt tatcttatac ttgggtataa gggacaatat ttccgccgca ttgaatttgt
    21541 cgtaatcaaa tgcaaaaacc gcagcctcca tcagccactg tccgtaaagt ttgccccaga
    21601 cggcttttcc ccatccggta tcggctatgg taagatgcag tccttcggga tggacattat
    21661 gccagtgctt tgcggtcaga agatgtgcca atgaatatgt atggttatgc agtaccattt
    21721 tgggataacc ggaagtgccc gaagaaaaat acataagcag cggttctttt acatgcgttt
    21781 ccactctttc aaagtattca tcggccagtt gcacaccttt gtcgaaatcg ctccatccct
    21841 ctttatgacc gtgtgccatg atacggacgg aaagagaggg gcaaatattc agaacatcat
    21901 ctatagcctg tgcggcatgt ccctcacccg tgcatatgac cgcctttacc gaagcggcgt
    21961 ttatcctgta ctcggcatca tgggcggtta tcatgaatgt aacgggaatc attattgctc
    22021 ccaacttatg cagagctacc gatgtatacc aaaactggta atggcgtttc agaatgacta
    22081 ttaccttatc gcctttctct attcccagcg accgtaaata gttggcggtc ttgtcggaca
    22141 gcctctttat atctgcaaac gtatatattc tctcttctcc ttcgggattg caccacacaa
    22201 gggccttcct gtggggatca tttattgcta tgtcatccac cacatcatat ccgaagttga
    22261 agttgtccgg gtacttaagc ttaaaactct taagcacacc gaactcatca taactttcat
    22321 caacatatct caaatttata tttctcatta ccgctcctta cttacatcct tcatcactat
    22381 tgcaagaaaa acacattcct tccccaaagc cgccgtcata ccgtgttcta ttccggaatt
    22441 gtagtagacc gaatctccgg catacaacat ttcctcacgg tcaccgtgtt ggaatttcaa
    22501 tgcaccttcc aggatatagt cgaactcctg accttcatgc ttattcatct tcagaggctc
    22561 gttatactcc ttctcgttat aaggcgctct tactaagaaa ggctccgcgg tcttgttttt
    22621 aaaattggga gcaaggtggc tgtataagaa gtactctctt cttttaatgg gtaatccttt
    22681 acccttccgt ataattgcat actgtccgag atgcgggttt tcccctgtca atatttccac
    22741 tatatccaca ttgaactttt ccgcacatct gtacaggaaa gtaaatgaat agtcggaaga
    22801 tttgccgctt tccagcttaa tgtaatcctc aaccgataca ttggtagcgg acgccatctc
    22861 ctctacagta aaatcaaata tttccctgag tgttctgatt ctttgggcta tttccgatat
    22921 ttccagttcc atgacaacct ccgttctgtt acccctcatt ttacatatat atgcatacaa
    22981 atgcaataaa aatgaatttc cggtacttat ttacacatat atattcaaat taacaaaaat
    23041 aaatgatata ctacggagta aataattttt aagtgcgggt aatatgaaga gactgaagaa
    23101 aatcataatc atatgcggac attacggaag cggaaaaacc aattttgccg tcaaccttgc
    23161 cgtggattta agaaagcaag gatatcccgt aacactgacc gatatggata tagtaaatcc
    23221 ctacttccgc tcttccgacc atagtgaaat gttggattca aaaggcataa aggtaatagc
    23281 ccccgtttat gccaacacca acctggatgc tcccgcaata ccggctgaaa tgtattcggc
    23341 attcgacaga tccggatatt ccctgttcga tatgggagga gacgatgccg gtgcaacggt
    23401 aatggggcag tttttccgca acattacagg caaagaatat gatatgcttt atgtaataaa
    23461 cggcaaaaga gccatgatag aaaatgtgca tgatgctgcc gagatgcttt attccataga
    23521 gtccgcttcc cgattgaagg cgacatatat cgtgaataat acgcatctta aagactatac
    23581 cgatgccaag acgatacttg agtccatgga atatgcagag aaagtatcgg aagcggttaa
    23641 cttaccgata ttgtgcacca catatcccgt aagtatgatt aaggagcagg aaatatcggc
    23701 aacgggggga ataataacac agcatcttta cccggtggaa gtgtttgtaa aaacgccgtg
    23761 ggaagttaat tgaaggaggc cgaaatggca aaagcgataa tagatgagac taaatgcaaa
    23821 ggttgcggtt tatgtgtagc agcctgtccg aggaaaatac ttgtactgtc acccgataag
    23881 attaacagca agggttaccg tcccgcacag gttgtcgatc agtcaaagtg taccggttgc
    23941 gcatcctgtg caataatgtg ccccgatgtg gcaataacgg tggaaaggta ggataatatg
    24001 gcaaaagtat tgatgaaagg taacgaagcg ctggctgaag ccgccattat ggcgggatgc
    24061 aggcacttct tcggatatcc cataacaccc cagacggaat tggctgccta tatggcaaag
    24121 aaaatgccca agataggcgg tgtttattta caggcggaat cggaaattgc agcaatcaac
    24181 atggtcttgg gagcatccgc cgcaggaaaa agagcaatga catcctcttc ttcaccggga
    24241 ataagcctta aatcggaagg tatctcctat atcgccggaa gcgatttacc cgcagtgata
    24301 gtcaatgtac agagaggagg accgggctta ggaggcatac agccttcaca ggccgactac
    24361 tggcaggcta ccagagccac gggacacggg gattttcatc ttatggtata tgcaccttca
    24421 acggtacagg aaatgaccga tctggttgct ctggcatttg acaatgccga caaatacaga
    24481 atgccggtta tgatactgtc cgacggtatg ttaggacaga tgatggagcc ggtggagctt
    24541 aaaactgcta atgcgaccga ttgccctata gaaaagccgt gggcggcatg cggtcaccga
    24601 aacagcagac ctcacaatgt aataaactct ctttatctta cgccgcaggc attggaagag
    24661 ctggtgaaga aaagatatga gaaatatgag gttattaaaa aagaagaaca gatggcggac
    24721 gaatacctga ccgatgatgc cgatataata ctggttgcat acggagcttc gtccagaata
    24781 tgcagaaccg cggtaaatac ggcaaggaaa gaaggaataa aagcgggact tatccgaccg
    24841 ataacattat ggccctatcc cgctaagtat atagaaaaac atatcgctca tgcaaaagcc
    24901 ttcttatgcg tggaaatgag catgggacag atggtggatg atgtccggct ttgtgtcaac
    24961 ggaagaaaac cggttgcctt ttacggacgg acgggcggta taatacccgc tcccgacgag
    25021 gtattgaatg agataaggaa attaacggga ggtgtcaagt aatggctgta gtatttcaaa
    25081 aaccccaagc actgttggat gtacccttta catattgtcc gggatgtact cacggaataa
    25141 tacacagact ggtagcggaa actatagacg aactgggaat agaaggtcat accgtgggag
    25201 tggctcccgt aggctgttcg gtcatgaact ataacttttt tgcctgtgat atggtacagg
    25261 ctccgcacgg cagggctcct gcggttgcaa ccggcttaaa aagagccata ccggaaaata
    25321 tagtgttcac atatcaggga gacggagatc tggcggcaat aggaacggcg gaaaccgttc
    25381 atgcagccgt aaggggtgaa aacatcacgg tggtattcgt gaataatgcc atatacggta
    25441 tgaccggagg tcagatggct cccaccagtt tacccaatca ggtaacacag accactccgt
    25501 acggaagaga tacttcttat tccggtcatc ccataagagt atgtgagatg ctggcaacac
    25561 tggaagggac ggcactggca caaagagtct cggtagataa cgtaaagaat gttcaaaatg
    25621 ctaaaaaagc aataaaaaag gcttttacct accagataga aaaaagaggt ttttccatag
    25681 tggaagtgtt atccacctgc cccaccaact ggggattgga tgcacaggaa tctttacaat
    25741 ggctgagaga taacatgatt ccctactatc ctttgggagt atataaggat gtcggaggta
    25801 caacaaaatg agtacactga atattcttct ggcaggtttc ggaggtcagg gcatattgtt
    25861 cttaggtaag atggcggcgt atgcgggact tatggataac aggcagataa cctggcttcc
    25921 ctcctacggt ccggaaatga gaggcggaac ggcaaactgt tcggtctgca tatccgacga
    25981 cctgataggg tcgccgctgg ttataaatcc cgacatattg gtggccatga atcttccctc
    26041 tttggagaaa tttattgata aggtagtacc cggaggcacc gttttagtgg attgctcaat
    26101 aatagacaaa aaagtgcaca gagaagatat aaatgtcttc tatgtacctg caacacgcct
    26161 ggcggctgat aacggattac cgggactggc caatatgata atgctgggta aaatgctgaa
    26221 agaaacaaaa ttctgcacgg aaaacgcatt ggaggaagct ttattgaaat gtgtcccgcc
    26281 aagaaaagcg gacatgatgg aactgaacag aaaagcgctt aagatcggga gtgaactgta
    26341 atggaagaga caatggagat tgcaagaagg attgctggta taagagatgc ctgcggatat
    26401 tcacaggaag atatggcaaa agagttgaat atcgatattg aaacatatcg cgaatatgag
    26461 aaaaacggac agaacatacc catttccgtc atattccata tagccaataa gttcagagtg
    26521 gatctttccg agatacttac cggaacatcg gcaaaactgg atacatacca tatggtaaaa
    26581 gccggacacg gcaaggaagt ggacagatat cccgggtaca actataccga tctggctttt
    26641 cgctattcga aaaagataat gcaaccgctg ttggtaacac tggatccttc ggatgaaccg
    26701 gccgctcttg taagccatac cggtcaggaa ttcaactacg ttatcaaagg taaaatggca
    26761 ttttcttttg atgatcaaca gataatatta gacgaaggag actgcattta tttcaatgca
    26821 actcactctc acggtcaggc ctgcgcatcg gatgtgcctg tagtatttct tacggttata
    26881 gcggaatagg agtaaatatg ttagaaaaat accttcccag aacggaattt gcctcctatg
    26941 aagacttcaa gcaaaattac aaggtaattg ttccggacaa tttcaatttc ggatttgata
    27001 tagtggatgc atgggcagag caggataaag acatgccggc attggtctgg tgtgacgact
    27061 tgcaaaacga gatgcatttt acttttgccg acattaagaa actgtccaac cgggcggcca
    27121 attacttcat aagtttaggt ataaaaaaag gatctacggt acttgtcatc ttgagaagaa
    27181 gatgggaata ctggataata gccgtggcat tacacaagat aggtgccgta ctcatacccg
    27241 cttcgttaca gcttaccaaa aaagacataa cttacagggc aaatgccgcc gatgtgcaag
    27301 caatcatttg cttaaacgat gatttcgtaa tagcacagac cgaaggcgct tttcccgatt
    27361 gtcctacggt taagaacctt atacttgtaa acagggataa caacactgat agaaacaagg
    27421 gaagtactgt aagtgagata tggaaggatt tttctgaaga ggctttcaaa tgctccgatg
    27481 tattcaaaag acctgcagga aaagaggcta caaaaactac agacaccatg cttttgtact
    27541 tcacttccgg tacaacggga atgcccaaga tggtacatca cgattactcc taccctttag
    27601 gacatatagt tacggcaaag tactggcagt gcgtggaaaa aggaaaactg catatgagcg
    27661 tatccgattc cggctgggca aagttcggtt ggggcaagat ttacgggcaa tggatatgcg
    27721 gtgccgtcat attcgcatat gacatggaaa agttcatacc cgccaatctt cttaaaatga
    27781 tggaaaagta cagactgacc acattctgtg ctccccctac catgtttcgg tttatgcttc
    27841 aggaagatgt aaagcaatat gatctttctt ccataaaacg tttctgtatc gcaggggaac
    27901 cgctcaatcc ggaaatattc agaaagtgga aagagattac cggaaaacct cttaccgaag
    27961 gcttcggtca gtcggaatct tccgtaatga tagccaattt taagtggatt gaacccaagc
    28021 ccggctccat gggaaaaccc gctcccctct atgacataca gcttataaat gcggacggta
    28081 ttgcctgtga agacggtgaa gaaggagaaa taaccgtacg caatatggaa agcgagcatc
    28141 cggtaggatt gttcttaggg tattataaag atacggagct tacaaagcag cacattcaga
    28201 acggagtgta taaccttaaa gacgtggcat ggcgcgacaa ccagggatat tactggtttg
    28261 taggaagaca tgatgatgtt ataaaatgtt cgggatacag aataggtccc tttgaagtgg
    28321 aaagcgccct cctggaacat gaggccgttg tggaatgtgc cgtaaccgcg gttcccgatc
    28381 aattgagagg ccagatagtt aaagccacgg tagtactgaa aaaaggttat acaccgtcgg
    28441 cagagctcat aaaagagctt cagaaccatg taaagaaagt aacagcaccg tataaatacc
    28501 ccaggattat agagtttacg gaagagttgc ccaaaaccat aggcgggaaa ataaagaggg
    28561 gcgagataag gaacagcgat aaggaaggta gcaaatgaaa gttttatcta aaatatttat
    28621 aattctggca attttactgt caaacgttat gtgtgccgta gtcgcatata actattgctc
    28681 tcttgaatgg ggagcaaaat atgccggata cagcgcacct ccgggtgcag ccttagcact
    28741 tgctatccct tatgccgcag gtattgtcat actcatagta ttagcgataa tctttaacag
    28801 aaaagccggt aagaaatctt aatgtaattt gaaagagtac ttggacaaat acgacgtagc
    28861 tctgacattt caaacatatt ccttaacagt ttttatgaca tttcaaacat attttgcagc
    28921 gttttctctg actttttaaa aatactgcca tattcttttc tatgaaattt atggcatcta
    28981 tacaattttt ttgttcaata gattattagt aatagggtac aagttgaaaa acactgatat
    29041 gacgattaca taagggagtt gcattatagc ctccggatgc agttacagta atcttgtatt
    29101 gcagcttgaa tgtgaaaaat tatttaaaca caaagcaacc tgttatttct ttcttaaaga
    29161 tgcacttata aagtaatgtt tactgttctg ctttttaccg tcgaaatagc agtcatcagt
    29221 atgccggatt ctatctatac cgtaattgtc aaacaaagaa aaaatatcat caagttccac
    29281 ataaaagtgc ggtatgcctt tctccggtcc ttcttttgtc ttgattacgg tattggcatc
    29341 ccgtttggga ttatcggtat ccttaaaaga tgccgactct tttgaacaca gtgtaacgta
    29401 tatttcaccg ccgattttca atatccttga aatttcttta attatttctt taatccccga
    29461 agaatcggta tgagatataa catgatatgc aaaaatcgca tcaaagaagt tatcttcgta
    29521 cggaattttc atcatatcgg catttataac ctcaatagac aggttttctc tttccgccca
    29581 tgctttcagg tactccgttg cctcaaggga taaatcaaaa gcactgactg aaaacccctg
    29641 ttttgcaaaa aatattgaat gtctgcccaa tccgcaacca aaatccaaaa ccctctttat
    29701 attcctatct ttccaaagct gtgccacata atagctttct tcactcggtt gcagccaaac
    29761 ggaattcaat tccttgttcc aatcccatgc ttttgatatc gtcatgtaca atacctccta
    29821 cctacaacat tcatttcagc ataccaaacg gatgcataca gccttgaacg gagcaaatcg
    29881 aaaaaactat tctcaccccg ggacagtcta acactattca atataaatca tctcataatt
    29941 gatgagaaca aaaagggcta tgataaagtt attaacatag aataactccg aaagtatata
    30001 cttccatata tttatgtcac gataatcaat aataggtttc tataaccagt tttccctttt
    30061 cccggagctt ttccgctata agcttgatcg cagttcgtct tactctcact ccaacctgtt
    30121 taaacagcag aacttcgtga gcatcattca tagcattgcc gatcatcata tggatcacat
    30181 ctgcctcaag catcatcatg cataaacgtt cagccgcaga gttagcggta aaattttcaa
    30241 cgggttcatc gagtatgtta tatacctgat taagtataat agcaccttcg ccggtaaaat
    30301 ctattccttc tatggcatat tcaggaggaa ttccgaaggc ggtgcccatt ttgagtgttt
    30361 ctacttttct tcctgtctca cgtgcgacta tatccgcagt ggttgatccg cagattgctt
    30421 ttttgccggg catggaaatg aatctttcta catattcctt atccatcgaa actttcgatg
    30481 gaggaccggt catcacggta agttcgtttg cctcttggca atgcaatacg gccagagtag
    30541 tatcatcctc atatctgccg ttcataagtc ttgcacacat atcggttatc ttttccggca
    30601 ggtcatgtat atctgttcca gattgcaaca acttgtttat ataatctgaa atattctcaa
    30661 taccgatccc aaatccgtac ccatgcccca gtcctgcctg tgttacaccg tctgagcaca
    30721 gaactaacga atcacctatg tccaatacac ctactgtttc tcccacgact tcataccctg
    30781 tggtaaaaaa ctccggtttc agaacagatg acgttccgtt cttgataata atagcttgcg
    30841 gagcctcgta ggtatatatg gaaaactgtc cgtcaggcaa gattgccact gcagaaaatg
    30901 ctgaaaaagg atgatcttcc gttcttgctc tttgcatgga cgctgcaacc gtctccactg
    30961 tcgaacgtaa tgagacaccg agcttaaaca attcaactat cctgtcggaa caggaaatag
    31021 cggagatatt tgcatatatg cccgagccga taccatcaca cagagcaaaa acagtaccgt
    31081 taactgacct gtgacattta gtatgatcgc cgcatacaat ttcgccggtc ttaatttttt
    31141 gtgatatttt atcctcgtaa aatctcattt ttttactcat ttgttgtcac taatatttgc
    31201 atcgctttta tacaaatcaa taagacgttg tgctatctct tcactctgtg ctgtactttt
    31261 accgagataa tgagccatct cctgtgcaaa tttgacctga tgctcaagaa actcccgcgc
    31321 atgtgcaact gtctgagcct tgataagatc aagctgacta tcatcgaact ttatttttga
    31381 tatattcgag aaaatgccta caaactgttc ctcatcacgt aatgcatata aaatctcatg
    31441 atattttatc ccatatttaa cttgtatgga ttcatggtgc tcaatcgttc cggccagcag
    31501 tttctcaaat ccgtcggcat ttataagata agagattctt ctcccgagca gcccgttgtt
    31561 gcacataaac atcttctgaa aggcagggtt catttttatt atacaaagtt ccttgttgag
    31621 tatcactacc ccgttcggag aggtctcaat tatcctgtca gacctctgtt gagcaagcct
    31681 tcgcatatag ggaatgcaca tctcgggttc cgccataccg cgaaccacag cctttgcatt
    31741 ctcaacacaa ctcttatacc cgcaggctcc gcagtcaagc tgaaatttgg gatcggcctt
    31801 acccgtgtgt tcaaacacct tgtatatctg ctcttcagta accgtgtctt cattgacctc
    31861 agcatcggaa aattccgtga aataagcgat atctccaatg ctatcggttt gtttttttct
    31921 tttagctttc tccgtgtact caattatatc cagtcttctt tcaaaaacat ttttatcact
    31981 ctcaaaaccg ggaccgttta cacacccgcc tttgcagaaa agcggctcaa tcttgctata
    32041 acgaagcaat gtttcggggc tgtccagaag cgtcatcacg tcttcggcac cgctgacatg
    32101 cagaacatca gcggccgtaa catcactgtg tataccgccg gtcttgaaca tcccgccttc
    32161 aagaggaaaa agcctgctgt ctccaatttc accaatgcat tcaaaatcac tttccgaaca
    32221 attctcaagg tgaatatcct catcttcgaa ccatttcctg atctcggtaa aagtaagaac
    32281 aacatcaata acgcctgcgt tttcaggacg cagagcttcc tctttttttg ctgcgcacgg
    32341 tccgataaaa accaccgcat tatcaggata tttttcttta atcatcctgc cgtgagcaat
    32401 catcggtgat gttactctta taaggagatt gataagttca ggcttgtact tctcgatata
    32461 actcactacc acgggacacg ccgtacatat ggtacctttc tcgctttttg caaaagattt
    32521 atctgtaatg tagcttgcac cttcggctgt ttcaaaaacc atgttaaatc cgagctgcct
    32581 gagtgcggaa ggcagtcgtg ccgcaagttt tccgctaaaa agagcggcaa atgaaggcgc
    32641 aatgctgact gaaacttttc tgcctgaagc aatcaattct ttggcttcgt taatacttga
    32701 acgtattacc ttagcatgtt gtgggcattc tcttacacaa gtcccgcatt tgatacataa
    32761 atcgtcctga atttgagctt gaccgccact gactttaatc gctttgacag agcatgagct
    32821 tacacatcgg taacagtcct gacaatctgc agttacggta tatatcgtat tttgtctcat
    32881 cggtttaata tctccattgt attttgctac atctttaatc gaagaaccgc ttctatctcc
    32941 gccacagcca tttccaaagt gcagtgctca atattctttc cgttgataga tattacgggt
    33001 ccttttttac actgttttcc gcagaatgaa gcggtaaact cagtttttcc aagcaactcc
    33061 ctctctgaaa gatattccat taactgagtg tagagggttt gtgctcctct cagaaaacaa
    33121 cttgttccga aacaaatggt tatctccagt ttcttgtttt caggaacttc gttgattatt
    33181 atatcatcct gaagtatacg tcttctgttt tcatacgtag tatgcagcaa tctgtgtgcc
    33241 ttatcatcaa ccagtgtttc gttatagagc tgctttatat acggattctc cgacgacaca
    33301 tggaattgaa gcattttatc attgtcatac aatccttttg cgcggtcagc aaccgcatta
    33361 cttgcgtttg ataccggctg tccccctccg tttatacaac cgtcgcagca cgccataacc
    33421 tccacaagat cataatgttc ttcgcctttc cttattttat caattacttt ttttgcgttg
    33481 gcaagtccgc ttactacagc aagacgaagt ttcttatcgc caatctcgat ttccgaaacc
    33541 ttagtcctgt caccggttct gaattgcctg aactcgccga caaaaccttt ctgaagcgaa
    33601 tatgcggcaa atctcaatac cgcctcgctt actcctccgg aagctccgaa aataacgcct
    33661 gctccggtgg taaatccgaa aggcatatcg aaagaactaa gttcaagttt gccaaattca
    33721 attccgcttt ccttaatcat aatcgacagt tccttagttg ttattacata gtcgacatcc
    33781 ggattgccgt ttgttttaaa ttcatccctg cgtgcttcaa attttttggc agtacacggc
    33841 attattgaaa caactaccag atcctcgcga gaaattttaa gatctttcac aagttgctcc
    33901 ttacacaccg ctccgaacat ttgctgtgga gaacggcaag tagaaagatt tgacagcaaa
    33961 tcagggtaat attgctcggc atattttacc catgccgggc aacaggaggt aaacaaaggc
    34021 agattctcgt ttttggagta tctgtcaaga aattccctac cttcctctat caccgtaaga
    34081 tctgcgctga aagatgtgtc ataaacttta tcaaagccca ttctgcgaag tgctgcaatt
    34141 atttgaccgg tggttgtcgt gccgggtttg tatccgaaat attcaccaag tgagactctt
    34201 acggcaggag ctatctgcgc aacaacggtt ttacttttat catgtatggc ttcccacaca
    34261 tcgtttatat gatacttggg catcagggcg ccgacagggc aaacttttac gcattgaccg
    34321 caattgacgc attccacttc tccaatccct ttattgaacg gggtgaccac tttggcctcc
    34381 gcgccgcgat aagcgaaatc cagagcgcct acggactgta tctcattgca gacacgtacg
    34441 caatcaccgc aaagaataca cttacccgga tcccgtatga ttgcatcaga agacaagtca
    34501 agttcttggt gcgtcttcat ttgcttaaat cggattttat ccacgccaag ctgtttggca
    34561 atgttctgta gtttgcagtc accgctcttg ggacatgtgg tgcaactttg atcatgactt
    34621 gcaagcataa gttcaattat catcttgcgc atcttgcgta tctgcgcagt attggtatgt
    34681 actaccatac cgccctcagg cttggtcgag caggcaggta caattcctct cccctctatt
    34741 tcaaccatac acatacggca cgcgccatat acgctgattt ccgaatggta gcaaaatgtc
    34801 ggaagctcta tattcgcttt gcgaatcagc tccaaaaggt ttttctcatc ctcgatctga
    34861 attgtaaagt tatctattac ggcagtttga gttttcatat acattgattc atcctttcac
    34921 aacagcatta aatctgcagg cgttaatgca cgctccgcac tttatacatt tttccgcatc
    34981 aataacatac ggaacacctt ttgccccttc aatcgcctcc acagggcact ttttcgcaca
    35041 tagtccgcag gctttgcatt tctctgctat tattctgtac gtcttgagag cttcacaatt
    35101 acccgttggg catctcttgt caaaaacatg ggcattgtat tcatctctga aatactcaag
    35161 cgtagaaaga acggggttcg gagcggtttt gccaagcccg cacagtgaac ctattcttac
    35221 ggtttcagcc agctcttcaa gaagttcaag cgtctgtgca tccgccctac cttcgattat
    35281 ttcatccaat aaagcaagca tctgccgcgt gccttcacga cacacaacgc actttccgca
    35341 ggattcattc tgtgtaaact gcatgaaaaa tcttgcaatc tgtatcatgc aagtattttg
    35401 attcattacg acaagtccgc ctgaaccgac catggctcct acattacgga gattatcgta
    35461 atccagagga aggtcaagat gctcttttgt cagacaaccg cccgatggtc cgccgatctg
    35521 cacggctttg aaatccactc cgcagttatt tccctcatca tcaagtactc cgccgcctat
    35581 atcacctaca atttctctga gagttgtccc gaacggaacc tctatcagcc cggtgtttgc
    35641 gacatgaccg gtaagtgcaa aggtttttgt tcccttggat gtttccgttc ccaattcccg
    35701 gtacttggcg gctcccattt caagaataac gggaatagtg gcaagtgttt caacgttatt
    35761 tataacggta ggttttccga acaatccttt ttccgcggga aacggcggtt tcatggtcgg
    35821 catacctcgg tggccttcaa tagacgcaat cagtgcggtt tcctcaccgc aaacgaatgc
    35881 tcctgcgccc tcaattacct caatgtcaaa tttcttaccc gaaccgaaaa tattttcacc
    35941 cagtattccg ttttcccttg cggatttgat agcttttttc agcctttgga cagccagagg
    36001 atattcggtt cttacataga ccatacctat gctggcattt accgccatgg cggcaattat
    36061 cattccttcc agaacactgt gaggattacc ttccattaca catcgatcca tgaatgctcc
    36121 gggatctccc tcatcgccgt tgcatatgat atatttggtg tcactttcat tgatgcgcgt
    36181 cagttcccat tttcttcctg tcggaaaccc gccgcctcct ctgccccgaa gccccgaatc
    36241 cagaatttcc ttgcatatct gttccgcggt catttcaagc actgccttgc gtgccataaa
    36301 atatccgccg ttggcgatat attcatccac atcattgaca ttgatatgtc cgcaaagctt
    36361 tagcataatt cgtttctgtc tctcataaaa aggtatctta ttcatatcct ttacgtgatg
    36421 ctttgtatcg tgacactcat agagcagtct gtccacagac tcaccttttt gaagagttct
    36481 gtcgacaatc tcttccacgt ccgacacttt aacttttgta tacagaatgt tctgcggata
    36541 aatggttaca agcggtccca tctgacagaa accctgacag ccgctttgca ccaacaggaa
    36601 attgttattg acagtctgtg attcgcccgc tgcggcatgt ttatcgttat gcgcaacgtg
    36661 atgatgttct tccttttcaa gtacaacatc cgcttttaga ccgcgctctg caataacttc
    36721 tctgaatttg ttgtaaatcg acattgcacc gtttgccata caaccggttc cggcgcaaat
    36781 aactatccgc atcttatatg ctgtgcatgc tttgttataa ttttctctgg tcttttcaag
    36841 atctataatc tgttttgtca acattgaagc gttcatttat tcatctcctg tctttcttgt
    36901 tcacggatac ggcttatcag ttctatcgtt ttctcttttg tcatagagcc gtgtacttca
    36961 tcatttacta caactacggg cgcaagtccg catgctccga gacacgcaac ggtttcaaga
    37021 gtaaagagca tatcatcgga ggttttcttt ttaccggata atccaagctc cttttccagc
    37081 gtttgcagaa tgtctttgga tttcctgaca tgacaggcag tgccgtcgca tacctttatc
    37141 acatatttac ccttaggttc aagggcaaaa tgcgaataga acgtcgcaac tccgaatact
    37201 acgccaggcg atattcccaa tgccgttgca atatatgtca ttatgtcttc cgggagatat
    37261 ttgtactctt cctgaatctc ctgcagaatc gctataagtt tgttgggcga acattcgtgt
    37321 ttatccagaa cctcgcaaac cttatcaaat tttatcgaaa aagtatttct gtcatttccc
    37381 attcttaaaa accgtccttt ctccgtagta tgtatttcgg tatatatctt caaaatcaag
    37441 ctgaggcagc tttgccatac ttatttctcc ttttggtcaa gcctttttat cagcactgca
    37501 agtgacgcag ccatattctc gtttttaact actacatcat cgggtaactt caaatttacc
    37561 ggtgcaaaat tccatattgc ctttattccg cattcaatca tcatgtcaca taccgattga
    37621 gcatgttccg caggtactgt aataatgccg atcttaatat taaggcgttc acacaggttt
    37681 ttcattttct cgcatgcaaa tatttttttg ccgttaattt cagtgcccac aagttccggg
    37741 gagatatcaa aggcagccac tatatccaaa ccgtaattct tgaaattatc gtagctcata
    37801 agtgctttgc cgagattgcc cacacccgcc agcacggcgt ccgaagtgtt gtcgtgtccg
    37861 agaaaatgtg aaatatcggc cataagctcc tttgtaacgt atcccgtttt aggtcttcct
    37921 ccattactga cacttgccaa atccttccta accagtacat cgttaagctc aagtgccttt
    37981 gcgattgtag tagcggatat gttctccggt gcatcagctc ctaagccttc cagatagtag
    38041 agatattttg tcaatcggtt gtagagctgc agtgatatag gttgcttcat ttatatttca
    38101 actcctttca tgtaagtagt caggtttgca agccatattc tgtgctttga attttgcaga
    38161 cagtatctta catcccgttt caatacaaag atattgatat atttgtatcg atatatttgt
    38221 atcgatatct ttatatcatt taaccccctg agttgtcaag cgttttgtga aaaaatatgc
    38281 ataaaaataa ttttcgttac tgttcacagg gtagattttt ctaaaccata aatgcagcat
    38341 caggtatctt atctattcat tttcttaaaa tataatcatc tgtattcacg gcttgctcaa
    38401 agagcatgct cttattcctg cgataaacaa aatcatccga tgcatggcag gcaaaagcat
    38461 tattccacaa tacaaacatc tgtaaacata tgagtagcaa tatagtaaag cttaatcttt
    38521 tttttctcat tgtactcttt atcacagcac tatcttaaca atgtctcctt cagcactttg
    38581 tacatccacc agttcaaacc gtccgtattt ctttttaatc ttcctcactt cagccatcag
    38641 cctgcttaaa tccttacctg ataggttaaa ctcgtgcggc tcggcattcc tgctgatggc
    38701 tttaacggct attttggcac ctaagtcgga aaaaacaaac catgtgggga aaataagatt
    38761 tatcttttta ccgtcatccg atttcacctt tatacgcatg tcattctacc actatatcca
    38821 cggtatctcc gtctacactt tccacttcca caagtttacc taccacacct ttatccgcaa
    38881 gcgctatcag ttgagcaaaa tccacatctt tcaatgcttc atttcccgat acttccggca
    38941 tcttgagtcc catctccaat cccgctttaa ccagcggcat gggcaggttg acttttacct
    39001 tatcaccgtc tttggagttg acataaattc ttaataccag atcgtctata ctctttctct
    39061 tttcttccgg tacaagctcc acacttttct tcggctgatc ggacaacaac tcgtccactg
    39121 taaccgacag catttgtgct atcttgggca acagcataat atccggacag gatatatcgt
    39181 tctcccattt ggataccgcc tgagctgtta cattgagctt ttccgccatc tcatcctgag
    39241 tgatgttttt tcctcttctt aattccgcaa ttctgtttcc taatgttctc atcttttctt
    39301 ctccttatat aatatatatt tttctttgcc cgtattcaac acttacaaag ctgaataccg
    39361 gcacctttca tgattaaatt ttagtatcgc cgatacaggt attgaactta tcagcggttg
    39421 tttttattgt tttttcacaa cctgcggttg tgtatttact caactaacgg ttgattttag
    39481 tatttaaatg tatgtattga gttttcagtg ttgtcatttc atcttttaca tagaatttgc
    39541 agacaatcat atcaagtcat agtaaaattt agacagtata aagaaacggg acacggtact
    39601 tatgaaagtt atactgcacg acttggattg gcaatacagc caacggctat tgacaaaatg
    39661 cgatgcgata gtggaagcgg acggaaaata tgccccctgc aaaggttgtt tcggttgctg
    39721 ggtcaaacac ccggcggaat gctttataaa agataagctt cagacggcat gccgcatttt
    39781 agggcgtgcg gaccagctgg ttattataac aaaaaacctt tacgggactt acagtaccaa
    39841 gattaaaaat gtaatggatc gcactatagg gacttccacg cccctttgcg tttatcggca
    39901 acgggagatg caccatacac tgcgctacgg aaagcatgct cttctgaaag tgcttgtata
    39961 tggcgacatc acggaacggg aaaaggctac tttcatttac atggtaaaaa gaaatgccgt
    40021 aaatgacgga tacaataaat ccgaggtgct gtttttccac tcggctgcgg aagcggagcg
    40081 tgccgtatga aaaccgtact cgttaattgc agccccaaaa agcgcttcag tgcatcagca
    40141 taccttgcgg cattgcagag tttttttctg aagggtaaaa aggtcacaaa aaacttaaga
    40201 agcaaaaagg attatacccc tgtttttgag caactgaaag atgcggactc gttagtgctt
    40261 tgtcttcctt tatacgcgga cggtccccct tcacatgtat cggctttttt ggaggaggcg
    40321 gaactattct tcttaaagaa caatctgcat cccaatcttt attgtattgc caataacggg
    40381 ttcatagaag gacgacagaa tgaagcgctg atgcaggtca tggaaaactt ctgtcataga
    40441 gccggtatta actggtgcgg aggtgtaggg ataggaggcg gtgtcatgct taatatcacc
    40501 cgcatagttt ttgtgattca gacggcgatt ttctttatac agtacataat cggcattata
    40561 caaacgggac ttttcttaaa caccggtctt ctttatgctt ttttacagaa cgccggactt
    40621 ctcctgtttt ttaattccgg agtactgttc tttcttatcc ggatgggatc ggctgtcaat
    40681 aagtgctccg tttcaggtaa acactatacc cgtatattgc tcccctcctt tatattcatc
    40741 ctgattgcaa atatcttctt tacattactc tccctctttc agggcggtct tttcagaggc
    40801 tggcttaaga aaaagtagat atggttattc aacacctgtc gcgtctatat atcagcatat
    40861 gaaaatattc atatttatgc atattttaat atatttattc atttatatat tgactttatg
    40921 caattttgct gtataattat gctaattaaa cagaaagagg ctgtgcaatg aataaatttg
    40981 atgagaaaat aaagaaaata gtactggcat attcgggagg attggacaca tcggtaataa
    41041 taccgtggct gaaagagaac tatgaaggct gcgaagtaat agcggtctgc ggagatgtcg
    41101 gacaaggagc cgaaaccgaa ggactggaag caagagcact tgctaccgga gcttcaaaac
    41161 tatatataga agatcttacc gaagagtttg tatatgactt catattcccc accttaaaag
    41221 ccggtgcggt atatgagaag aaatatttat tgggcacatc ttttgcgcgc ccggttatcg
    41281 ccaaaagaat agcggaaatt gcattaaagg aaaatgccga tgccatatgt cacggctgta
    41341 ccggaaaagg caacgaccag gtacgcttcg aactgaccat caaagccttc gcacctaaga
    41401 tgaagataat agcaccgtgg agaatctggg acataaagtc gaggaaccag gaaatagatt
    41461 atgccatagc gcataatgtc ccgcttaagg tatccagaga aaccagctac tccaaagata
    41521 agaatctctg gcatctttcg catgaaggac tggatctgga agatccctcc aatgagcctc
    41581 agtataacaa ggagggtttt ctggagatgt gcgtctctcc ggaacaggct cccgataaag
    41641 cggaatatat aacggtagat tttgaaaaag gaattccggt agcggtaaac ggtaacagga
    41701 tgagcggacc ggatgtaata aaaacactca atacaattgg cggaaagcac ggaataggat
    41761 tggtagatat ggtggaaaac cgtcttgtgg gaatgaaatc cagaggcgta tacgagacac
    41821 cgggaggaac catactgtat catgcacatg aggtgctgga atcgctcact ttagaccgcg
    41881 acacctcgca ctataaggcg cttgtggcgg agaaattcgc agagctggta tatttcggcc
    41941 agtggtacac cccattaaga gaagctctgt cggcatttgt agacaagact caggaaaatg
    42001 taacgggaca ggtaaaactc aaactgtata agggcaatat tatcaatgcg ggaacggttt
    42061 ccgactattc cctttacaat atggattttg ccacattcga agaagacaat gtttacaacc
    42121 agaaagatgc ggagggtttc ataaatctgt tcggactgcc cataaaggta aaagcgcttg
    42181 ccggaggtta aaatggaaaa actttggaaa ggacgtttaa acagcgatac ggacaaactg
    42241 gcggataagt tcaactcttc catagatatt gaccatatcc tatacaaaca ggacataacc
    42301 gcctcaatag ctcatgcgga aatgctgggt aaaaccggta taataccgtt ttccgatgca
    42361 aagaaaatat caaaatgtct taaagaaata ttaaaagaaa cagatgacgg cagtttggat
    42421 atagataggg gttttgaaga tatacatacc tttgtggaat acgagcttac aaaaaggttg
    42481 ggtgatacag gtaagatgct gcataccgcg cgctccagaa acgatcaggt cgccaccgat
    42541 ttcaagatgt atgtaaaaga gcggatatcc gatgtgattg aatacatcaa agaactgctg
    42601 ggtgtattgg ctgatcatgc atcggataat atccacaccg taatgcccgg ctatacccat
    42661 ttgcaaagag cacaaccggt tacattgggt cattacctta tggcatatgc gatgatgttt
    42721 taccgtgatt tgaaaagata tatgtctttc gattccatga tgaatacctg tccgctgggt
    42781 agcggtgccc ttgccggaac cacatataac accgacaggc atatgaccgc tgctgcttta
    42841 ggctttgaaa tgccttcact caatagtatt gacggcgtat ccgaccggga tttcgcgaca
    42901 gatgcctgtt atgccaacag catggtaatg ttgcatcttt cgcgtttctg cgaagaactg
    42961 atactgtggg cttcgtggga gtttaagttt gtggagctat ccgacggttt ttccaccggc
    43021 tcttccgtaa tgcctcagaa gaaaaacccc gacatagcgg agcttatacg gggaaagagt
    43081 gcaaggactt tttccgatct gaatactctg ttggttatgc aaaaatcctt gcctttggca
    43141 tacaacaagg atatgcagga agacaaagag gcggtattcg acagtttcga tacggtaagc
    43201 tcctgtctga aagtttttac cgctatgttg aaagagacgg tcttccttaa agataatatg
    43261 aaaaaagccg caaataaagg gttcataaac gcaaccgatt gtgccgatta tctggtaaag
    43321 aaatctctgc cttttcggga tgcatacaaa attacgggac agttagtggc ttattgcata
    43381 caaaacgatt acacactgga aactctgcct ttggaaatct acaaaagcca ctcgtctttg
    43441 ttcgagcagg atgtgtataa ggagatatcg ttggatacat gtgtggaaag aaggatatct
    43501 tacggaggtc cttcggcaca atcggtactc ctacagatag agcatatcag ggaggctgtg
    43561 aatggttaag gttttcattg acggctctta cggcaccacg ggattaagaa tacatgaaag
    43621 gcttgcaaca agggcggata tacagttcat tacccttccc gaacagcaaa ggaaagatgt
    43681 aaacaaaagg gcaaaagcct taaacagtgc cgacatagct tttttgtgtc ttcccgaccg
    43741 ggcggctata gagtcggtct cccttattaa caaccccgat accgtggtaa tagacgcttc
    43801 caccgcgcac aggacagctc ccggctggac atacggtttt ttggagatgg gagaggatat
    43861 atacgataaa gttaagcaaa gcaagcgtat ctgtgttccc ggctgccatg ccacgggatt
    43921 catagcattg gtaagaccgc tgatagaaaa caatatagta caaaagaact atcctttttc
    43981 ctgcacttcc ctcaccggtt attcgggagg aggaaaagcc atgataagcg attataccga
    44041 ccaaaacaga gactgttcgc tgtcggtacc gcgtatatat gcacttaccc agtcacataa
    44101 gcatctgaag gaaatgaaac actattcttc catatccgac tatccccttt tcaatcccgt
    44161 tgtatgcgac atatacagta tgatgtgtgt aacggtcaat atatcaataa agggtgtaac
    44221 gattgacgat atatgcgata tttttgtaaa gaaatacggc aacagaccgc tgtttgatat
    44281 ttcggacata aaaggagaaa gctataacgg ttacatacct tccgatgatt tcaaagataa
    44341 ggattctata aaaatagttg ttacgggaaa cgatgaaaga atatcgctaa tcgcacttta
    44401 cgacaatctc ggtaaaggag catcgggtgc ggcggtacag a
//
LOCUS       DAOSOM010000016        44279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_902_length_44279_cov_7.602863, whole genome shotgun sequence.
ACCESSION   DAOSOM010000016 DAOSOM010000000
VERSION     DAOSOM010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 44279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44279
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_902_length_44279_cov_7.602863"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..2030
                     /locus_tag="PLF14_05225"
     CDS             222..2030
                     /locus_tag="PLF14_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HOT70730.1"
                     /translation="MSFFSILTIFAGLTLFLYGMNLMGIGLERVAGRKLESVIEKATS
                     RPIKGVLIGAAVTAVIQSSSATTVMLVGFVNSGLMRLSQVVGVIMGANIGTTITAWIL
                     SLVSVDSSNFIVRIFKPDTFAPILGIIGIGLVMAGKRNKNKDVGRILLGFFILMFGMT
                     TMSDAVKPLAQMEGFTKMLTMFSNPILGILTGAIFTAIIQSSSASVGVLQALSTTGVV
                     TFGSAVPIILGQNIGTCATALISSIGTNKNAKRAALIHLFFNVIGTVLFASLFYGLNA
                     VFDFAFVNSVLNPVGIAIFHTIFNVLTTLVLFPFTKLLVRLSETIIKDKGTVVEKIVL
                     DERFLNTPSIAVEQCKRYIVTMANVTKETILMAIPLVKHYDAKKAQVIVDNEDLIDTY
                     EDKLGSYLVKLSGRELNDKDSKEISKILHSISDFERISDHAVNILDVAKELYDKDITF
                     SEQAYKELDVMASAVEEILDLTVDAFLNNDFEKASKVEPLEQVIDELKIILKNRHIER
                     LQGGGCTIELGFILSDLINNYERVSDHCSNLAVLTIQIAEGLFDTHEYLQNVKQSDRT
                     HYMKYYRKYHDKYDVARIVDNNIKNEDPVALPKQKP"
     gene            2152..2820
                     /locus_tag="PLF14_05230"
     CDS             2152..2820
                     /locus_tag="PLF14_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT70731.1"
                     /translation="MMLNKILIVEDEPALRNLLKTCLVKEGYQVLEAKDGQEALDIFY
                     GENISLVILDIMLPKLDGFEVLRTLRLESDVPVLILTARNTEYDELQGLRLGADDYIT
                     KPFSPAVLVSRVKAVLKRAGIFADNDITAKDILMKIKEHEVYVKGEKINLTPKEFDLL
                     QYLIQNKNIVHSREKLLSAVWGYDFFGDARTVDTHMKCLRTKLGDSADIIKTVRKYGY
                     KLEV"
     gene            2843..4351
                     /locus_tag="PLF14_05235"
     CDS             2843..4351
                     /locus_tag="PLF14_05235"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT70732.1"
                     /translation="MLIILCILLINIIATVLFSNVVFERFYINAKYNELKDYAGKINK
                     YFAEMDISLPDFRANLINAFDEIERNNIEIIWFIYNPTINHADIGYATKITGRYLAND
                     PPDTKEVEEYISALINSGELNSIENVYEYKTSDINAGKHNINLITLVFENHDVRSNNM
                     LQSYIVLSSPKEYIATTAKTVERFVLHVSLVTLLMGVIAVVIISAQITKPIIKMNKVA
                     NKIAKLDFSESCEVKGKDEISALAGSINEMSAKLQESIDYLQQRAQLLKRDLEKQEGS
                     NKLKKEFIASVSHDFKTPLSLIIAYSELLTDKYKDDEEAKNQLMIITDQAVKMNLMVN
                     QLLTLSQLESGTIKKEDSIFCLSEAVESVVKDLHIMIEQAQINLTFNYQDDYIVKGDY
                     NKITHVITNLLENAIKYCDEKKIINININEIDGKIRTSIYNTHRGIPEQSLPNIFNSF
                     YKEDKSRGGSSKSYGLGLSIVKAVMEMHNGKYGAYNSNAGVVFWFELEKQDI"
     gene            4453..5718
                     /locus_tag="PLF14_05240"
     CDS             4453..5718
                     /locus_tag="PLF14_05240"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOT70733.1"
                     /translation="MNNEYKTSENNPTNLYYQYNNMHNNRQDKKMTSRKTLVTAVVLC
                     SIFSLLIGIAGGFIAGRLHKGVFEWRYEFTPQESSSVIERERPRNNDKTPIYSTSEIE
                     KQYASVAEIYADSVDAVVGIVVETESGSGWSTYTQTGSGSGVIISEDGYIVTNNHVIE
                     NAKKITVYLNDEESYTAKLVGLDVQLDLALLKIDVDGLDYMVCGDSDDVMVGEVVLVI
                     GNPLGYLNQSLTVGYISGLEREIVLGNEKMVLLQTDAAVNPGNSGGALINIKGELIGI
                     INAKSYGSEIDGIGFAIPINDIKDVLDDIVNFGYVKGRPFLGVGLMDVTSQSTLNWYN
                     MMYGMNLTNLGVNVTKVQEGSAADKAGMKMYDCIIEFNGVSVQSASHLKELIDSCTVD
                     QAVEIVVARGNETLTLTAIIGEEIPETSR"
     gene            5724..6464
                     /gene="truA"
                     /locus_tag="PLF14_05245"
     CDS             5724..6464
                     /gene="truA"
                     /locus_tag="PLF14_05245"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOT70734.1"
                     /translation="MLKNILLTIEYCGKNYSGWQRQLNGVSVQEKIEDAIEKLTGQKI
                     GINGSGRTDAEVNALGQCANFKIDCTIPPSKFGMALNSMLPEDIRIVNSEEVDMDFHA
                     RYSATGKHYRYTILNRTAPSAIYKHTTYHMKHPLNVDKMIKAGSYFVGKQDFRGFMSA
                     GSKIKKTVRTIYSLDITKDGDLIIIDIKGDGFLYNMVRIIAGTLTEVGRGNIPVEEVK
                     DIIQSRNRSRCGPTLKASGLCLMEVYYD"
     gene            6595..7131
                     /gene="raiA"
                     /locus_tag="PLF14_05250"
     CDS             6595..7131
                     /gene="raiA"
                     /locus_tag="PLF14_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOT70735.1"
                     /translation="MKVSIVGRRVDITEEIKDWTEKKFAKLDKFFNGNTEVTVVVEAL
                     KQNKHTKVEATIVWKGVIFRAELSGNDLLGQIDKSVDIIERQVRKNRTRLEKRFHGDV
                     PDNWQGITQSAEYSEAAYNVVRTKRFPVKPMSVDEAIMQMEMLNHEFFMFVNEETNEM
                     NVVYKRKDGDYGLLEPYR"
     gene            complement(7214..7960)
                     /locus_tag="PLF14_05255"
     CDS             complement(7214..7960)
                     /locus_tag="PLF14_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007390795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOT70736.1"
                     /translation="MAKNITKWEPEDFELETTTHWSFPNRGNWATHDAKWRGNWSPYV
                     PRNILLRYSQEGDLVLDQFVGGGTTLVEAKLLNRNIIGVDVNDSALARCREKIDFKHE
                     GANGKVYIRKGDARQLDFIPNDSIDLICTHPPYADIIQYSENIEEDLSNLKVNDFIKE
                     MKAVAAESYRVLKKNKFCAVLMGDTRQKGNMVPMSFSVMRVFEESGFKLKELIIKEQH
                     NCSATGFWKTNSVKHNFLLIAHEYLFIFRK"
     gene            complement(7960..8496)
                     /locus_tag="PLF14_05260"
     CDS             complement(7960..8496)
                     /locus_tag="PLF14_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006193363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA amidotransferase"
                     /protein_id="HOT70737.1"
                     /translation="MSKNEFKYNVNLVPLRIENQKQNAFRDELNNIPYLLYKVENLAN
                     GEIIAINKPGGKRNFGRLSRDDFMVFIYNPHEQSLWLISHGEISDDIAKKYDYNQNHA
                     LLVIKGLYKVCCGLEPDDVIADLCLGDTVGIPIETILKVYKWIWGQEDCNYPTKEGRW
                     LSMNALLNRFGLNVEDFR"
     gene            complement(8504..9385)
                     /locus_tag="PLF14_05265"
     CDS             complement(8504..9385)
                     /locus_tag="PLF14_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005357465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HOT70738.1"
                     /translation="MADRDFNAWLLSFRNSIADYGYYIDFKKVHKNIDNIKVELNILN
                     SLIGSKNIEVDFENLIKKYPEVLKCIPLLLAVRANEIYCQDENGGYLFQFDPENIQLK
                     NPTQYERYKYFMKKTGLFDLLKKHVVNNLIDYATGVETGLDSNGRKNRGGHLMENLVE
                     SFIKKAGFVKDINYFKEMYIQQISEKWGVNLSAISNQGKMEKRFDFVAKTPTMVYGIE
                     TNFYSSSGSKLNETARSYKNLAMETAVVTGFTFVWFTDGKGWISARNNLEETFDAMEH
                     IYNIKDLEDGIIEEIMI"
     gene            complement(9398..10468)
                     /locus_tag="PLF14_05270"
     CDS             complement(9398..10468)
                     /locus_tag="PLF14_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007390779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HOT70739.1"
                     /translation="MEFMSAKEAADKWGISQRRVAYLCSEDRIDNVAMVGNMWIIPVT
                     AKKPIDARSIRYTKNNEKKIKPFLKWAGGKGQLLSEIEKYYPFKKGKITKYAEPFVGG
                     GAVLFDILSKYNLEAVYISDINAELINTYRIIRDDIDELVDMLTVMQKEFVPMSTDSR
                     KKYYLEKRFRFNHLKLNGNENVNVEKAALMIFLNKTCFNGLFRVNRKGMFNVPIGSYK
                     NPLICDRENLHAVSEKLQNVTIVCKDYRESANFIDKNTFVYIDPPYRPLTDTANFTAY
                     TENLFNDGEQIELAKFVDAMHRKGAKIVVSNSDPKNSDTEDNFFDNIYSSHNIKRVEA
                     TRMINCNSEARGKIKELLISNF"
     gene            10617..11570
                     /locus_tag="PLF14_05275"
     CDS             10617..11570
                     /locus_tag="PLF14_05275"
                     /inference="COORDINATES: protein motif:HMM:NF020804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HOT70740.1"
                     /translation="MQSERLDYALNMLLEGEDINLLKKHAERISEDYRDGTGPSKRIV
                     NDAKKAKAYAASRMPATYRAVYDSLRYVISVSDLCVSSVLDVGAGTGAGIFACTDLLQ
                     AEDITLLEREESMISIGKQLFSCDSLLSSARWIKASVQDIGTDKKYDLVTASYVMNEL
                     NEEDMLRTAEKLWDITGDTLLIVEPGTPKGYANILVIRDILLQKGAYITAPCTHAGKC
                     PMVEDDWCHFYCRVQRSRLHKQLKGGEAPFEDEKYSFMAFSKKEAQLPEFRVLRRPRI
                     YKGHIMLTVCSKEGIQNITVTKKEKERYRDAGKLKAGDAFY"
     gene            complement(11564..12667)
                     /locus_tag="PLF14_05280"
     CDS             complement(11564..12667)
                     /locus_tag="PLF14_05280"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT70741.1"
                     /translation="MAAQLNTRLRFVRTLTGQEVDRVPFMRVFGGTNHALPSWLEKRP
                     NLSRYMDQLIGFEGTYRGWAIVPANYNLCGLPEDICIEDRGSVRIYRNGIGEIRTWSK
                     DSDYHSGVIEYALKTADDWEKIRPYVVCDVEKRIPRDIDELAEIYKNRDYPLQLTCGG
                     VYGFIRRLAGDENLGYMFYDSPDTVKEIITEYINMLLELWERLCDKIRFDLIESWEDM
                     AYKCGSIISPEHFNEFLAPHYRTIRAFADAHDIPILLVDSDGNTDLLAEWMYAAGVNA
                     LYPFESLAGCNPANVRKKLPGMGGIGGLNKDCMAYGKKAMDEELEKAEALIKLGRFIP
                     GPDHMVLSNVTFENYRYFMSRLKDIVMNTKPNQ"
     gene            12804..15065
                     /locus_tag="PLF14_05285"
     CDS             12804..15065
                     /locus_tag="PLF14_05285"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOT70742.1"
                     /translation="MSLKSNNIADNLNEEQLKAVTRTEGAVLVLAGAGSGKTRVLTRR
                     IAYILEQGLARPSQILAITFTNKAADQIKQRVYALCEEDKKIYFPWIGTIHSCCLKML
                     RQYPEDAGYRNNFLIYDTDDKKSVIKNCMKQLNISDKAFPVAYVASEISRAKDDLLGY
                     KEYAAANANDFQKKAVAGIYELYQIKMKEYNAMDFDDIILNTYFLLKDNRHILEYFQK
                     IFKYILIDEFQDTNIAQYRIFAMLSMKHKNLFVVGDDDQSIYGFRGANVKNILGFEKD
                     FPDCTVIKLETNYRSYANILTAANAVIKNNVARKSKTLKTSKPEGSRINVYKAYNELN
                     EAVYVANEIKKLMEKGYSCKDFAVLYRTNAQSRVFERIFRERGLNYRIFGGLSFFSRQ
                     EVKDVLAYMRVVFSTDDDASLFRIINQPKRGIGDTTVKRIQSIAAREYLSSYEIVKNA
                     DRYEQLSSAAEKLKAFSDMIEQLKEYNVPGQLEQLYDNILKLTGIHEYYISEDVNFGR
                     ERVENIKELKSAIVTMNQSQQEDFGELLSLETFLENVTLATDADKEEESDDYISIMTL
                     HSAKGLEFRVVFLVGMEEGLFPSNLSMSEGAHGLEEERRLCYVGITRAMDILYCIHAE
                     ERTRYGKTEPCEQSRFLKEIPKELINSGKRAFSRREKSGSSFDWFTSSQPVSVTKGGK
                     PVNIPGLKTSTEASVKKQDYSIGDRVMHKKYGEGSIKDLYDDNGMDIVEVNFDMAGVK
                     RMVLEFAKLVKIQ"
     gene            15178..15651
                     /gene="nrdR"
                     /locus_tag="PLF14_05290"
     CDS             15178..15651
                     /gene="nrdR"
                     /locus_tag="PLF14_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOT70743.1"
                     /translation="MKCPNCGHEEDKVIDSRPSEEGYAIRRRRECLNCKERFTTYENI
                     ERKPIIVVKKDQSRQNFDKAKLLNRLMIACAKRPVSINKLEDIVNAMELDFHKRHLRE
                     VTSREIGDKVLEHLIDIDYVAYVRFASVYKDFDDMDTFIKEINLVKEKHKKQETE"
     gene            15652..17091
                     /gene="proS"
                     /locus_tag="PLF14_05295"
     CDS             15652..17091
                     /gene="proS"
                     /locus_tag="PLF14_05295"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011722820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOT70744.1"
                     /translation="MTQGKKDVEQITGRDIDFAKWYTDVVTKAELIDYSQIKGCVVLR
                     PYAYALWENIQKILDDMFKKTGHENVYMPMFIPESLLQKEKDHVEGFAPEVAWVTLGG
                     SEELTERICVRPTSETLFCDHYKNIIKSWRDLPKKYNQWCSVVRWEKTTRPFLRHREF
                     LWQEGHTIHATVDEAREETHRMLDIYADFCENYLAMPVVKGQKTEKEKFAGAEETYTI
                     ECMMHDRKALQSATSHYFGDGFAKAFDIRFTDKDNTLKNPFQTSWGMSTRIIGGIIMT
                     HGDDSGLKLPPKVAPVQVMVIPIAQHKEGVSEAADALLEKLKLSGLRAKTDVSDQSPG
                     WKFAEYEMKGVPLRLEIGPKDIQNNQCIAVRRDTKEKIALSLTDIDKSVKELLDKIQK
                     NMFEEAKANLDNNTFVAYSVEEMRDIIQTKGGFVKAMWCKDLSCELELKEKALVSSRC
                     IPFVQEKLSDCCVCCGKPAKHMVVWAVAY"
     gene            17098..19479
                     /gene="priA"
                     /locus_tag="PLF14_05300"
     CDS             17098..19479
                     /gene="priA"
                     /locus_tag="PLF14_05300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOT70745.1"
                     /translation="MKSVTVAAVILDNTTRTFDKVYNYLVPEDMYMYLETGMRILVPF
                     GRADTKKVAYFLNFVPYTEKLKDIKMKYIAEILDDEAVLSKNMFILAKQIRDQYFCTY
                     GQVLSAMVPLGLKVSVEKLILQEDGTLTLFNTYLHSLEGGIKELNRLLKEDRIKIVEQ
                     GVRNIKKKFEKAARLKITSEQVANLIYGDVIKNERHLRVLDLLSTYEDITVNDFSCYE
                     NISRNILSTLAKKGYIELFDKPLKSDTVSEEIDEIQPSDKIPTAEQAYVTDTVSLSIE
                     KGIYDKYLIHGVTGSGKTEVYLNLAEKVVNKGKDVIVLVPEIALTPMMVRRFTQRFGN
                     NIEVMHSRLSMKERYNAWMKIKRGEVKIALGTRSCIFAPFDNLGLIVIDEEHDSSYIS
                     DRTPKYHTNFVADKRAQMEKAVLVLGSATPKITTYKVFEKVGKVLTMTNRASGKLPEV
                     TLVDMREDAINGKLSVFSTTLLNEIKKNLDNKEQTILFLNRRGYSSIQLCTSCKAVVK
                     CQHCDVTLTYHKGKNNLICHYCGHMQKKSNICPECHEEAIMEYGTGTEKCEEQLHELF
                     PEATIIRMDQDTTGMKNSHKEILERFEKEKIDILIGTQMVAKGHDYPLVTLAGILNAD
                     ALGCGAFYESNENAYQLITQSSGRAGRADLEGRAIIQAYNVDSPLITGAIRQDYKSFY
                     LSELKVRKVLSLPPYTNMAFLIFSSSERHLSEEYADIAYRLLSKTSMTVYAPSKAGIA
                     KLHDKIRTRVIVKHKDTKEFMENITKAYLFMIKKLPGKVTMSIDVFKDEFDVL"
     gene            19544..21208
                     /locus_tag="PLF14_05305"
     CDS             19544..21208
                     /locus_tag="PLF14_05305"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008399388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOT70746.1"
                     /translation="MLTDIEIAQNAAIKPITKIAKSIGIKEESLELYGPYKAKVKSSA
                     LAGKEQDGKLILVTAITPTPAGEGKTTTSIGLADALRKIGKKSMLALREPSLGPVFGV
                     KGGAAGGGYAQVIPMEDINLHFTGDIHAITAANNLLAALVDNHIYQGNKLKIDPRLVT
                     WKRCLDMNDRQLRYTIDGLGGKSNGMPRQDGFDISVASEIMAILCLSDSIEDLKERLG
                     RIIIGYDFEGEPVTASMLNAQGALAVLLKDAIKPNLVQTLEGTPAFVHGGPFANIAHG
                     CNSIMATKYALRLSDYLVTEAGFGADLGAEKFCDIKCRITGLKPDAVVIVATVKALKY
                     HGGVNKKELGIPNAEAVEKGMPNLYKHIENITEVFKVPAVVAINKFSKDTQEEIDVIK
                     KGCASVKVKAVECLVWEKGGEGGIELAKEVVRLCRKRSRFEYCYDSDLSITEKIKAVA
                     TKIYGADGVEFTSEAKAVLKIINSKGYNNLPVCIAKTQFSFSDNVKLINRPKNFTITV
                     RDLRISAGAGFIVALTGEIMTMPGLPKVPAAEAIDIDNEGKITGLF"
     gene            21253..23574
                     /locus_tag="PLF14_05310"
     CDS             21253..23574
                     /locus_tag="PLF14_05310"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HOT70747.1"
                     /translation="MADLMMKRIPASDWQDALPLGNGYVGAMVYGNIQKEKILLNHER
                     LFRYQKQPDYEPFYKYLPHVRKLMLEEKYSEGEKYFNEKFKECCKEVYNVDPYQPFCE
                     ICLYRKPDETFTDYSATLDFVSAVATVRWKEKDTEIIRENFVSYDTGCICTSMFSSDN
                     RPLDLKITVDSVAGGENYVDSFTCIQDNCLVFNSQYKDGRKHGAVIFVNTDKGSVEYD
                     DKFISVKAAGRIYLFVMLYIYEDEKKAINRLIKTVKQYDYSQLKKDHITVWNKLYNTA
                     SLTLGDETKLMSNEEILLEAYQNKKPADLFHTMFNYGKYLYISSSRENGLPVNLQGIW
                     NGSYEPKWDSDIHNDENVQMCYWASLPLGLFESSLALYDYFDSFIPHFKENAKKIYNC
                     RGILVPICMSTHGKICGTSGKWNSWIAAGGWIAAHYYDYWLFTEDEEFLKHRAVPFMK
                     QVVLFYEDFLITGDNGKYIFVPSISPENVPIHENRSMMCVNATMDVAIAKECITNLIT
                     ACKHLGIERENISKWEAMLSKMPEYEINEDGAMKEWLYDGLKDNYYHRHQSHIYPLFP
                     GNEITKENNPKIYDALKTAVDKRLLTGFVQQTGWSYAHMANIYARLEDQESALACLNY
                     ILRSCTGANLFTYHNDWRDQGLTMYPFGKNPPFQIDANLGFTAAMIEMLIFSKPGFIR
                     ILPALPDDWNKGKVKGLRTRCGCIADIEWDLKKKHITVKLASLKKEPVEIEITAGFKK
                     IECKEGTLLSKRDNRYLTVQSDKGKQVILEIGI"
     gene            23627..24553
                     /locus_tag="PLF14_05315"
     CDS             23627..24553
                     /locus_tag="PLF14_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HOT70748.1"
                     /translation="MKDKGFIIKQMEYFAEYRDREVFGMTPYEILEYKLFTLGYYHDQ
                     KGFEVPEDLFYCSYELIDQYMNKKGKVGYMRVYSPLEYYRACEDIKTEINISHIENGV
                     RILSVTETYIDYSAKIEPEAVIYPNCFIEGDSFISAGSLIGPDSKIVSSKIGGNTTVL
                     KSVVLQSTVGKNCSIGPFAYIRPDTTVKDNVKAGTFVELKKTVIDDNTTIPHFVYAGD
                     AEIGRHCNISCGVITANYDGKQKHKTKIGNNVFVGCNSTLVAPVELQDNTFVAAGSTV
                     VEDVKSGSLAIARAQQVNKEGWMEKTGRMKKE"
     gene            24570..25550
                     /locus_tag="PLF14_05320"
     CDS             24570..25550
                     /locus_tag="PLF14_05320"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOT70749.1"
                     /translation="MARNDIKIMGGNANLKLAAEIANYLRVPLLDSKVGRFADGEIQV
                     MINELVRGSDVFIIQSTCPPVNENLMELLVMTDALKRSSCARVTAVMPYFGYARQDRK
                     ARARDPISAKLVANLLTTAGVDRILTMDLHAPQIQGFFDIPVDHLEGLIIFSDMIADI
                     MKGQDKSDFILVAPDMGSVSRIRKMADLTDLQIAIIDKRREKANECEVMNIIGDIEGK
                     TAIIIDDMIDTAGTITKAANTVVDMGAKRVLVYATHGILSANAVSKIKNSKIEKMYLL
                     DTVPVPEEKMIDKLEIVNVAKLFSEAIYKIYTNTSMSALFKMPPSGRNEK"
     gene            25550..26134
                     /gene="pth"
                     /locus_tag="PLF14_05325"
     CDS             25550..26134
                     /gene="pth"
                     /locus_tag="PLF14_05325"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008395535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOT70750.1"
                     /translation="MDRYLIAGLGNIGLRYRNTRHNVGFMAVDKLADVYSIKVKKNLC
                     QANVGEGFINNIPVILAKPKTYMNLSGQSIRALTDWYKIDISRLIVIYDDMDIPVGEL
                     RIRYKGSAGSHNGMKSILSHLGTDRFIRIRVGIGKHEHIEAVDYVLGRFPKSEKEIID
                     NTLQDLTKAVTCIVTEGTDIAMNRYNKKNDRGDV"
     gene            26139..29558
                     /gene="mfd"
                     /locus_tag="PLF14_05330"
     CDS             26139..29558
                     /gene="mfd"
                     /locus_tag="PLF14_05330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOT70751.1"
                     /translation="MNPFIQVFSDIPQLIGVADCIALKQTPVGLSGPGTSLCPLISHI
                     TASSVSRKLLIVTHSRTAALSVHRNLIAFYGDDAVHIPARELMLFDIDAYSGNDKQAR
                     LKALYRIATGDFKAAVACTEALTVGTASRQVFLQNLINLKTGEQRDMETLGDMLMRLG
                     YVRKALVEEQGQYSIRGGILDIFSCNYDNPIRCEFFGDEIDSIRFFDADSQRSVEETD
                     NVVIIPYSEFILDRNDEKILKEKITDSWDLQQFNEGDYAKIMDRYYAATDKCAMTGTY
                     FDKDILTVIHDDLQCHSVADTVYKEYANACVNLLQKKKTFKEACEIQKDIYSLYSCLK
                     PQLILVYEKSNYSIPLYRDFDITFEETCSYKGDMELFLNDISEYRAANMRIFISSDRK
                     KHTEYLKGFFEDNGYTVHVIQPKENPTVIPRHIYIIEDGYGESFVSKEAKIACISVSR
                     IFRKSTRGKTRQIRDADFFAEIQPNDYVVHDHHGIGIYQGIEKIEIDGIIKDYIKIAY
                     KNNDMLYVPVNKMDLVQKYVGTSDRAPRITGLGGRDWEKQKSKVRSAVRDIAKELIEL
                     YGKRSSVKGHAFSEDTPWQISFENEFEYEETQDQIQCVEEIKADMESTKVMDRLLCGD
                     VGYGKTEVALRAAFKAIMDGKQVAFLAPTTMLALQHYNNFRERMKNYPVNIDLLCRLR
                     DKKRQTETVEYIKSGKTDIVVGTHRLLEKDVSFKDLGLLVVDEEQRFGVAQKEKIKML
                     KPTVDVLSLTATPIPRTMHMALSGIRDVSTIYDPPEKRFPVQTYVMEYDPEFIKQVIL
                     REKDRNGQVFYLYNRVMDMDSKFYHLKTLLKDQVRICVAHGQMPEKEFENTIIKFINR
                     EYDVLLCTTIIEAGIDISNANTLIIEDADKLGLAQLYQIKGRVGRSDRVAYAYITYKR
                     GKELTEEASKRLQTIKEYTEFGSGIKIAMKDLEIRGGGDLLGANQSGHMQGVGYDMYV
                     RILSEEIANLRGEKVKGAVTETSVDLNISAYIDKSYISDDLSRLKMYKRIATVATVTE
                     KEDIIDELTDRYNQPPGHVINIINVAYAKNLACRLGISEIVQTNKGFVMIYEKEHAMQ
                     FNEVTDILDKEHNKYKVKAQEDLQIRVDTDKKNEELLDFITKILEKAVESK"
     gene            29640..30845
                     /locus_tag="PLF14_05335"
     CDS             29640..30845
                     /locus_tag="PLF14_05335"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOT70752.1"
                     /translation="MAVAKGKKEDVKKKSSKEISKEETIIRYGFLTLAVIIGLCLIYF
                     VIASLIPNNYIKIGNHKIAEEEFMYQTNTQYKVFVNYYQDYIKNLGFDLSTPEGISAF
                     MKTAYDNQRSFGQMLLEGVVDSIQEQYIMLDQIKETGFKIDEEELEESFAIQMEAIQR
                     AADESGYDLDKYAKLFYGTTFKAMQRVIKQTLRAQQYTTYLEEQNISAVTEERARSYY
                     NSVDADGNAVRDDIDKLTVVTILKRTVDDEFQPLAEDVVAAAKVKAAEIFEMAKSGRD
                     IYELAKLYSDDLKDQETEESEEETEDTAKPGEFTFTKKEVGTTELTQWALKASVGDID
                     LIETEIGHVIIKLIDRTDYEDVKDDIFKLIASSDFEVKMNEYKDMEQYKVGLYKPYQE
                     LYLTYAITQ"
     gene            30997..31989
                     /gene="pta"
                     /locus_tag="PLF14_05340"
     CDS             30997..31989
                     /gene="pta"
                     /locus_tag="PLF14_05340"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HOT70753.1"
                     /translation="MTFLERTIQRAKSNKKTIVLPESEDIRTLKAASVALKEGIADII
                     LLGNKEKVKKNGKGLNIPEEKVLEIDKFDRIDEYANMLYQLRKEKGVTLDIAYKLLEN
                     PLYFAVMMVKMGDADGMVAGAVSSTSDTLRPALQILRTAKGTKIVSAFFIMCVPDCQY
                     GANGVFAYGDSGLVENPDAEQLSEIAISTAKSFELLVEEQPVVAMLSYSTYGSAKSEL
                     TEKVIQATKLAKEKAPELLLDGELQVDAAVVKEIGQRKAPGSSVAGKANVMIFPNLDA
                     GNIAYKLTERLAKAEAYGPLLQGIAKPVNDLSRGCTAEDIVGVIAITAVQAQSV"
     gene            32015..33217
                     /locus_tag="PLF14_05345"
     CDS             32015..33217
                     /locus_tag="PLF14_05345"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOT70754.1"
                     /translation="MKVLVINTGSSSLKYQMIDSENEKMLAKGLCERIGLPNGIHTYT
                     CADGSSITDECVISDHSHAFKLMLDALVCPATGVLKSISEIDAVGHRIVHGGERFTKP
                     ALIDEDVIKAIEDCVDLAPLHNPAGILGIRVCQKVLKNVPMVAVFDTSFHHTIPDYAY
                     MYALPYEMYEKYRVRKYGFHGTNHKYVAQRTAELINRPLDKIKIVSCHLGSGASICAI
                     DRGKSIDTSMGFTPLSGLAMGTRSGTIDPSIISFIMEKKNMTVEEVNYYLNNKSGLLG
                     VSGISSDFRDLVKAKDEGNKRATLALNIFAYRVKRYVCEYVGVMGGIDALVFTAGVGE
                     NNAYIRERITKNLDIFGIKMDRRKNNNAKGETDVSRDRSNVRILIIPANEELEIARQC
                     KSLLEDGK"
     gene            33290..33799
                     /locus_tag="PLF14_05350"
     CDS             33290..33799
                     /locus_tag="PLF14_05350"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOT70755.1"
                     /translation="MKIDITDIIERDGAVKHFVTDVDKLSDRNTGSGKSIVDVQGVIH
                     IEGEIYNHEGILDISAQIFGQLKAQCYRCLEDITAPFSVHMEETFERANGSKNLDNYE
                     YSGHILDISQAVIDSVYLNIPYRMLCKSDCKGLCPKCGSNLNQKECGCIEQSEENGRF
                     SALKDLFKE"
     gene            33824..34009
                     /gene="rpmF"
                     /locus_tag="PLF14_05355"
     CDS             33824..34009
                     /gene="rpmF"
                     /locus_tag="PLF14_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012938743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOT70756.1"
                     /translation="MAVPKRKVSKQRQRKRRTFYKLEEPNLVRCSQCHTLKRQHRVCD
                     ECGYYDGKEVIKKEEEK"
     gene            34045..35382
                     /locus_tag="PLF14_05360"
     CDS             34045..35382
                     /locus_tag="PLF14_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HOT70757.1"
                     /translation="MENKAIAIKQILKGSLAKRAGIKPGDFLLSVNGFGIKDIFDYQY
                     HISDESVLLEIINGKTGKLTGVEIKKDIDEDLGIVFEKELIDDEKVCRNKCIFCFIDQ
                     LPKGMRETLYFKDDDARLSFLTGNYVTLTNMSYEEIKRIASMHLSPINISVHTTDPEL
                     RCTMLNNRHAGDILDKMAVLAEAGTDLNCQIVLCKNVNDKAHLDRTIKDLSCIGQPLK
                     SISVVPAGLTRYRDGLYPLEPFTEEECIQIINQVQKHQKVFKEKSGYNLVYAADEFYI
                     KAGLELPSFGEYDDFYQIENGVGLMTHFIHDAKNYLEEISNSKKLLKRAEKNTKHKSI
                     ATGVLAYPFIKELTDKVSMYAKNMKIDVYEIKNNFFGENITVTGLLTGQDISEQLQGK
                     QLGEQLLISRTMLKASDEYFLDDMSYKQLGKKLKTTVTVTGNNGEEFIKTLIK"
     gene            35544..36824
                     /locus_tag="PLF14_05365"
     CDS             35544..36824
                     /locus_tag="PLF14_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009142534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOT70758.1"
                     /translation="MKYRKRIVDEQLDFKLEAFGATLIEGPKGCGKTTTAKQKAKSVV
                     EFQNEDERENYLIIANTKPSELLKGEKPRLFDEWQDAPKIWGAIRKDIDDTGGVGKYI
                     LTGSSSNTVKTPHTGTLRISRLKMYPMSLYESGESNGEVSLKELFTNPDSFDGCRSDL
                     TIDEIKFSICRGGWPGSLDRSTPKTQLAIAKDLFNQTCNVDISNIDGVNRNPQWAKAI
                     LKSYARNLCTLATTKTILADVNASCEMSKQSYYDYVSALEKLMIIEDIDAWNPAIRSK
                     TSIRSSAKRNFIDPSIAVAAFGISPEYFDRDYKTLGFLFESLCIRDLIVYSSNNDGNL
                     SYYRDRLGLEADAVLHLDDGRYALIEIKLGQSDVDESAKHLCEIETLIREYNESNKKV
                     SLNMPTLKIILTGTQYGYRRDDKVFVIPIACLRD"
     gene            36927..37223
                     /locus_tag="PLF14_05370"
     CDS             36927..37223
                     /locus_tag="PLF14_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOT70759.1"
                     /translation="MAAYTVSELKDIVGKLAAKYGAERVYLFGSYARGEAKPESDIDL
                     RIDKGSIRGLAFASLLGDLQDALGVEVDLISTAGMDNDFLREVQKDEVLLYERA"
     gene            37604..38926
                     /gene="der"
                     /locus_tag="PLF14_05375"
     CDS             37604..38926
                     /gene="der"
                     /locus_tag="PLF14_05375"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOT70760.1"
                     /translation="MAQPIVAVVGRPNVGKSAFFNYLAGRRISIVEDTPGVTRDRIYT
                     KCDWCGREFVLIDTGGIEIGSSDEMYKQMKRQAEVAIDTADFIIFMTDIKSGLTAGDK
                     EVASLLHKSGKPVVIAVNKADSPGEPPAELYEFYNLGLAEIFPISAMHGLGIGELLDY
                     VMEHLPDIQEQSDDDSIHVAIIGKPNSGKSSLLNRITGEERAIVSPVAGTTRDALDSL
                     VIREEKKYTFIDTAGIRRSSKIFSDIEKYSIIRAWSAIERSDVCILIVDASEGITEQD
                     SKIAGFAHEQGKAAIVALNKWDLVEKDNKTYQKFVNSVRAELPFMQYAPVISISSLTG
                     KRVENLFPLIDKVYENANRRISTGVLNDVLNDAIAMVQPPSDKGKLLRIYYMTQVSTQ
                     PPTFVIFVNSKDLMHFSYERYLTNTLRDTFDFEGTPIRFIIRERGKEN"
     gene            38923..39543
                     /gene="plsY"
                     /locus_tag="PLF14_05380"
     CDS             38923..39543
                     /gene="plsY"
                     /locus_tag="PLF14_05380"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014287194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOT70761.1"
                     /translation="MNILLHVISAVFGYLVGSLNSAILVSRFYKKDIRNEGSKNAGLT
                     NVLRVLGKKAAIAVLLLDLLKGVITTGLGYILVKDQLSGALVAGIFTVIGHIFPVFFK
                     FKGGKGILISLSVLLMTDWRIALIGLGIFIIIVGLTRYVSLGSITAMFLSPLFAWLLN
                     HEPFFIISMGFLAALTIFMHRSNVKRLLNGTENKLSFNKTGNGEGK"
     gene            39540..40541
                     /locus_tag="PLF14_05385"
     CDS             39540..40541
                     /locus_tag="PLF14_05385"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HOT70762.1"
                     /translation="MSNLIVINTSFNPWYNLALEEYLLDKVTDYDNILFLWQNANTVV
                     IGRNQNAWRECRTELLESEGGKLARRSTGGGAVYHDSGNLNFSFILPRSSQDFLIQAG
                     IITKAVGKFGINAYVNGRNDIVTDQGKFSGNAFTYRKNRALHHGTVLVDVDREKLGRY
                     LQVSKEKMQIKGIKSVQSRIVNLKEYDSGITVERVRDAIMQAFVEEFGQCEIADCSKE
                     IDIFDRKFDMAEVHRTYEIYSSWEFRYGEAPKFDIEWANRFSWGEVVIHLTLKNGVII
                     KSRIYSDALDELFIANIRQCFDNVPFKKKDMAEAVVKQHPDSPMAKDIAEYILSKEM"
     gene            40541..40933
                     /locus_tag="PLF14_05390"
     CDS             40541..40933
                     /locus_tag="PLF14_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HOT70763.1"
                     /translation="MFFKDLHIKDIADIPVLSLAYIGDAVYDLYVREHLLAKGCFKPT
                     DLHKMAVVYVNAKGQKQSLMKIMDILTEKELNCVQRGRNAKPRSKPKNADAADYSYAT
                     ALEALVGYLYINKEYERLDYLLGIMLDI"
     gene            40930..41670
                     /gene="rlmB"
                     /locus_tag="PLF14_05395"
     CDS             40930..41670
                     /gene="rlmB"
                     /locus_tag="PLF14_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOT70764.1"
                     /translation="MMEEKNTVIAGRNPVLEALKKDCLISRILVSVGEKEGSILKILS
                     EARKKNIPVKQVSNTKLDSIIPAHQGVVAYISPIEFYEISDLLDVAKERKEPPFIVIL
                     DQIEDVHNLGAIARSAEAAGCHGIIIPLHRSAPVNAAAMKASAGALLHIKVARVTNLN
                     AAIEQLKKQGLWICGTDKDANKQYYDADLKGPLAVVIGSEGAGMSRLVAKNCDFTVNI
                     PLSGNITSLNASCAAAIVIFEAVKQRKA"
     gene            41676..42179
                     /locus_tag="PLF14_05400"
     CDS             41676..42179
                     /locus_tag="PLF14_05400"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOT70765.1"
                     /translation="MEYLFVDGYNIINAWSDIFDKNSPLEDNRKALCDILSDYQGYTG
                     FRIVVVFDAHLVPEGIGSLMQYDKIKVVFTKKNQTADNFIERYVAKECYNNKITVVTN
                     DYMEQKTVLHKGALRMTASELQREIIKKRQSASTSDKQSNFIIDNLDNEQKRKLLKIK
                     HEDRKDK"
     gene            42157..42744
                     /locus_tag="PLF14_05405"
     CDS             42157..42744
                     /locus_tag="PLF14_05405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT70766.1"
                     /translation="MKTEKINSIIKKAKAGDEVSMEYLLKEYSSLVRKKATGLFLMGA
                     DKDDLLQEGMIGLFKAILNYDETKEASFDTFAALCINRQMFSAIKGANRKKHKLINEY
                     ISIYTEDKEDIPLAIPDEKYNPEVIVTETEALHNLENMIRNKLSSMESKVLDMLIEGH
                     SVAYMSKKTGKNAKSVDNAIQRIKSKVRNILKEEH"
     gene            42778..42853
                     /locus_tag="PLF14_05410"
     tRNA            42778..42853
                     /locus_tag="PLF14_05410"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42811..42813,aa:Thr,seq:ggt)
     gene            43154..43618
                     /locus_tag="PLF14_05415"
     CDS             43154..43618
                     /locus_tag="PLF14_05415"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOT70767.1"
                     /translation="MSVKFRFIHDDALGNDIEITVRAAEKNERVEKLLSKLAESSGQL
                     ISGQILSEKYDIDTSDIILVMRDGRYVTAKTVRGDYIIKDALTRVEESLNPTWFVRIS
                     QSEIINLKYLKNWDFVGGGIIQIKMENGIITHSSRRYATQIRDMLLKRRAKR"
     gene            43615..44097
                     /locus_tag="PLF14_05420"
     CDS             43615..44097
                     /locus_tag="PLF14_05420"
                     /inference="COORDINATES: protein motif:HMM:NF022896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HOT70768.1"
                     /translation="MKKRLIIRSLIGLVVGALAVHAITLFVNYISRGEILICMPELIE
                     KLGLTKAIIVQTVLGALISMIAIGGTVLFDIETWSLLRATVAHCALILFSYLTVGLLL
                     SWFSFHIIPILIMTGGIIIVYALIWLIMYAIWKAEIRNMNRLTEEYKKDIEASEDQSS
                     "
BASE COUNT        14645 a         7822 c        10034 g        11778 t
ORIGIN      
        1 tacgccgaag atacctggcc ggagacggcc cgggaaaata gggagctgcc ggacagctct
       61 tttctaaaaa cacacttggt gtgtttttta ttttgggcat ttttttacac tcttaatttt
      121 tatttaaatt tctctttact ttaagccggt tattatgaaa tctgtgttta tattatgtaa
      181 gaaatattgt ataataggcg taatttgaat gtgaggtgga gatgagcttt ttttcaatac
      241 tgacaatatt cgcaggactg acactgttcc tgtacggaat gaacttaatg ggaataggac
      301 tggaaagagt ggcgggacgc aagttggaat ctgtcataga aaaagccaca agccgaccga
      361 taaaaggcgt tttgataggt gccgcagtga cggccgttat acaatcttct tcagctacta
      421 ccgtaatgct tgtaggtttt gtaaactccg gactgatgcg cctgtctcag gttgtcggcg
      481 taatcatggg agccaatatc ggaacaacaa taaccgcctg gatcttaagt ttagtcagtg
      541 tggacagcag caactttatt gttagaatat tcaagccgga tacatttgca cctatactgg
      601 gtattatagg gataggtctt gtaatggccg gaaagagaaa taagaacaag gatgtgggca
      661 gaattctttt ggggtttttc atactcatgt tcggaatgac cactatgtcc gatgctgtca
      721 aaccgcttgc ccagatggaa gggtttacca agatgcttac catgttttcc aatcctatac
      781 tgggtatact tacaggagcc atatttaccg caataataca aagttcttcg gcatctgtcg
      841 gtgttttaca ggctttgtcc actaccggag ttgtaacatt cggaagtgcc gttcctatca
      901 tattgggtca gaatataggg acatgtgcga cagctttgat ttccagtata ggaacaaaca
      961 agaacgcaaa aagagctgcg ttgattcatc tgttctttaa tgttataggg actgttcttt
     1021 ttgcatcttt gttctacggt cttaatgccg tatttgattt tgcatttgtt aactcggtat
     1081 tgaatcccgt aggaattgca attttccata ccattttcaa tgtactgaca acactggtcc
     1141 ttttcccttt taccaagttg ttggtgcggt tgtccgaaac cattattaaa gataagggca
     1201 cggtagtaga aaaaatagtt ctggatgaac gctttctgaa tactccttcc atagccgttg
     1261 agcagtgcaa gagatatata gtaacaatgg ccaatgttac aaaggaaacc atacttatgg
     1321 caatacccct ggtaaagcat tatgatgcga agaaagctca agttattgtg gataatgagg
     1381 atctcataga tacctatgag gataaattgg gatcttatct tgtcaaatta agcggacggg
     1441 aattgaacga taaggacagc aaggaaatat ccaagattct gcacagtata agtgactttg
     1501 aaagaataag cgaccatgcg gtaaacatac tggatgtagc caaggagctt tatgacaaag
     1561 atattacctt ttccgagcag gcatataaag agttggatgt tatggcttca gctgtggaag
     1621 aaatactgga tcttactgtt gatgcattcc ttaataatga ttttgagaaa gcatccaaag
     1681 tggaacctct ggagcaggtg atagatgagt tgaaaattat tcttaagaat cgccatatag
     1741 aaagattgca gggcgggggc tgtacaattg aattgggatt tatactgtcc gacctcataa
     1801 acaattatga aagagtttcc gaccactgct ccaatcttgc ggttcttaca atacaaattg
     1861 ccgaaggctt attcgataca catgaatatc tgcagaatgt aaaacagtcg gatcggaccc
     1921 actatatgaa gtattatcgg aagtatcacg ataaatatga tgtggccagg atagtggata
     1981 ataatataaa aaatgaagat ccggttgcac ttcccaagca gaaaccataa agtacatata
     2041 taaaaggcta tggcggcaac tattgccatc ggctgcctta tcagtgataa aaacacagtt
     2101 ttatcacaaa tcggtgctat aattaactaa aagaaagcat tccggaggtt tttgatgctt
     2161 aacaagatat taatagtgga ggatgaaccc gctttaagaa atcttctgaa gacctgcctg
     2221 gttaaggaag gctatcaggt gctggaagca aaagacggac aggaagctct ggacatattc
     2281 tacggagaaa acatatcatt ggttatactg gatataatgt tgcccaagct ggacggattc
     2341 gaggtcttaa gaaccttaag gctggaatcg gatgttcccg ttttgattct taccgcaaga
     2401 aataccgaat atgatgaact gcagggctta agactgggag cggacgatta tataacaaaa
     2461 cctttttcgc cggccgtact tgtatcaagg gttaaggcgg tattaaaaag agccggaata
     2521 tttgccgata acgatattac cgcaaaagat atacttatga aaataaaaga gcacgaagtt
     2581 tatgtcaagg gagaaaagat taatcttacc cccaaggaat tcgatcttct tcaatacctt
     2641 atacaaaaca aaaatattgt gcattccaga gaaaaacttt tgtcggcggt atggggatat
     2701 gatttcttcg gagatgcaag aacggtggat actcacatga aatgtttacg cacaaaactc
     2761 ggagactcgg cggatatcat aaaaaccgta cgcaaatacg gatataaact ggaggtataa
     2821 aatcagactt cgcaataagc ttatgctcat aatcttgtgt atacttctta tcaatatcat
     2881 tgctacggta ttgttcagca atgttgtatt tgaacgtttc tatataaatg ctaagtataa
     2941 cgaacttaag gattatgcag gaaaaataaa taagtatttt gcggaaatgg atatttccct
     3001 ccctgatttc agggctaatc ttatcaacgc ttttgacgag attgaacgaa ataatattga
     3061 aataatctgg tttatatata atcccacgat taatcatgcg gatataggct atgcgaccaa
     3121 gattaccgga aggtatcttg ctaacgatcc tcccgacacc aaggaagtgg aggaatatat
     3181 ttccgcactc ataaacagcg gtgaattgaa ctccatagag aatgtttatg aatataaaac
     3241 cagtgatata aatgcgggca agcataacat aaatcttata acgctggtat ttgaaaacca
     3301 tgatgtgcgg agcaataata tgctgcaatc atacatagtc ttaagttctc ctaaggagta
     3361 tattgccaca accgcaaaaa ctgtagagcg attcgtgctg catgtttcac ttgtgacatt
     3421 gcttatggga gtaatagcgg tggttattat atcggcacag ataacaaagc ccataatcaa
     3481 aatgaataag gtggcaaaca agattgcgaa gttggatttt tccgaaagct gtgaagtgaa
     3541 agggaaagac gagataagcg ctctggcggg aagcataaat gaaatgagtg caaaactgca
     3601 ggaaagcata gattatttac agcaacgggc acagcttttg aaaagggatc tggaaaaaca
     3661 ggaaggctca aataaattga agaaggaatt tatagcatcg gtttctcatg atttcaaaac
     3721 gcctctgtcg cttataatag cgtacagcga gctgctgacc gacaaataca aagacgatga
     3781 ggaggccaaa aaccaactga tgattattac cgatcaggct gtaaaaatga atctgatggt
     3841 caatcagctt ctgacgctgt cgcagttaga gagcgggaca ataaaaaaag aagacagtat
     3901 tttttgcttg agcgaagccg tggagtcggt agtgaaggat ttgcatatca tgattgagca
     3961 ggcacaaatc aacctgacat tcaattatca ggatgactac atagtcaaag gtgattacaa
     4021 caaaataacg catgtgataa ccaatcttct ggaaaatgca attaaatact gtgatgagaa
     4081 aaagattatt aatattaata taaacgagat agacggtaag ataaggacaa gcatatataa
     4141 tacacaccgg ggaatacccg aacagtcatt gcccaatatt ttcaacagct tctacaaaga
     4201 agacaaatcc agaggcggta gcagtaagtc gtacggattg ggattgtcta tcgtaaaagc
     4261 cgttatggaa atgcataacg gaaagtacgg cgcgtacaac agcaatgccg gagtggtatt
     4321 ctggtttgaa ctggaaaagc aggatatatg agataagtcg gtgtgtttca catgattcat
     4381 aagatatttt cacaatttta tcacaagcga ccgatataat agggattgtc atcaatatga
     4441 ggaggatatg atatgaataa cgagtataaa accagtgaaa acaaccccac aaatttgtat
     4501 tatcaatata acaatatgca taataaccgg caggataaga agatgacatc aagaaaaacg
     4561 ttggtgaccg cagtagtgtt atgttctata ttttcactgt tgataggtat tgcgggagga
     4621 tttatcgcag gcagattgca taaaggtgtt tttgaatgga gatatgagtt tacaccgcag
     4681 gaatcttcgt cggtaatcga gcgggaacgg ccgagaaaca atgataaaac ccctatttac
     4741 tccacatcgg aaatagaaaa gcaatatgct tcggttgcgg agatatatgc cgattccgta
     4801 gatgcggttg taggtattgt agtggaaacg gaatccggtt ccgggtggtc tacttacaca
     4861 cagacaggtt cgggatccgg tgtcataata agtgaggacg ggtacatagt aaccaataat
     4921 cacgttatag agaacgcaaa aaaaataacg gtttacctta atgacgaaga aagctatacc
     4981 gctaaacttg taggactgga tgtgcagctg gatcttgccc ttttaaagat agatgttgac
     5041 ggtcttgact atatggtgtg cggtgacagt gatgatgtaa tggtaggtga agtggtgctg
     5101 gtaataggta atcctttggg ataccttaat cagagtctga cggtaggtta cataagcgga
     5161 ctggaaagag agattgtttt aggtaatgaa aagatggttc ttcttcagac cgatgcagca
     5221 gtcaatcccg gcaattcggg cggagcactc ataaacataa aaggtgaact tataggaata
     5281 atcaatgcca agtcctacgg aagcgagatt gacggaatag gttttgccat acctattaac
     5341 gatatcaagg atgtattgga cgatatagtc aatttcggat atgttaaggg cagaccgttt
     5401 ctgggagtgg gacttatgga tgtaacttcg caaagtactc ttaactggta caatatgatg
     5461 tacggaatga atcttaccaa tctgggtgta aacgttacca aggttcagga aggttccgca
     5521 gctgataaag ccggtatgaa gatgtatgac tgtataatcg aatttaacgg agtttccgta
     5581 cagagtgcaa gtcatctgaa agaacttata gacagctgta ctgtagacca agcggtggaa
     5641 atagttgttg caagaggtaa cgaaacatta acattaaccg caataatagg agaggaaata
     5701 ccggaaacat ccagatagaa tatatgctta aaaatatatt attgacaata gaatactgcg
     5761 gtaagaatta ttcgggatgg caaagacagc ttaacggagt ttccgtgcag gagaaaatag
     5821 aggatgccat agaaaagctt accggacaaa agatcggtat taacggcagc ggacgtaccg
     5881 atgcagaagt gaatgctctg ggtcagtgtg ccaattttaa gatagactgc acaattcctc
     5941 cttctaaatt cggtatggca ctcaacagta tgctgccgga ggatataagg atagtcaatt
     6001 cagaggaagt ggacatggat tttcatgcac gttacagtgc tacgggaaaa cactacagat
     6061 atacaatact aaacagaacg gcgccttcgg caatatataa acacactaca tatcatatga
     6121 aacatcctct gaatgtggac aaaatgataa aagcgggctc atattttgtt ggcaaacagg
     6181 atttccgcgg ctttatgtcg gccggaagta agattaagaa aactgtaaga accatataca
     6241 gtctggatat aaccaaagac ggagacctga ttattataga tataaaagga gacgggttcc
     6301 tgtacaacat ggtcaggata atagcgggaa cactgacgga agtgggcaga ggtaacatac
     6361 ctgttgagga ggtaaaagac attatacaga gtcgcaatcg ttcccgatgc gggccgacat
     6421 taaaggcaag cggcttatgc cttatggaag tttactacga ttaaattatt tgttaaaaat
     6481 attttaatta tttatcaaat atatgtaatt gatttattgt agccgggttt ttcatgcggt
     6541 ataatataag tatcaataca catatgaata catacaacga aaggagacag ttttatgaaa
     6601 gtttcaattg tcggaagaag agtcgatatt accgaggaga taaaagactg gacggaaaag
     6661 aaatttgcaa aactggacaa gttttttaac ggaaacaccg aggtaacggt agttgttgaa
     6721 gctttaaagc agaacaagca tacaaaggtg gaggccacca tagtatggaa aggagttatc
     6781 ttcagagccg aactttccgg taatgatcta ttgggacaga ttgataagtc ggtagatatt
     6841 attgaaagac aggtaagaaa gaacagaaca agactggaaa aaagatttca cggggatgtg
     6901 cccgataact ggcagggtat cacacagagt gcggaatatt ccgaagccgc atataacgta
     6961 gtaagaacaa aaagattccc ggtaaagcct atgtcggttg acgaggctat aatgcagatg
     7021 gaaatgctca atcatgagtt tttcatgttt gtaaatgaag aaaccaatga gatgaacgtt
     7081 gtatataaaa ggaaggacgg agattacggt ttattggaac cgtacagata aacaaaaaaa
     7141 caaccggctg aaaggccggt ttttttgaat ataaatatag ttaataggaa caaccattta
     7201 aagtaggata aatttatttt ctgaatataa aaagatattc gtgagcaatc aatagaaaat
     7261 tgtgttttac gctgtttgtt ttccaaaatc ctgtagcact acaattatgt tgttccttga
     7321 ttatcaattc tttcagttta aagcctgatt cttcaaacac acgcataacc gagaaggaca
     7381 ttggaaccat gtttcctttt tgtctggtgt cacccattaa gactgcacaa aacttattct
     7441 ttttcagaac acggtagctt tcagcagcga ctgctttcat ttctttaata aaatcattga
     7501 ctttcaaatt tgataaatct tcctctatat tctcgctata ttgaataata tcggcatagg
     7561 gcggatgggt gcatatgagg tcaatactgt cattaggaat aaaatctaat tgacgagcat
     7621 ctcctttgcg aatatagacc ttgccgttag ctccttcatg tttaaaatct atcttttctc
     7681 tgcaacgagc aagtgcgcta tcatttacat cgacaccgat tatgttcctg ttcagcaatt
     7741 tagcttctac aagtgtagtc ccaccgccaa caaactggtc aagcactaaa tcaccctctt
     7801 gtgagtatct gagtaaaata tttcgaggaa catatgggga ccagttacca cgccattttg
     7861 catcatgagt tgcccaattg ccacggttgg gaaaagacca atgtgttgtt gtttccagtt
     7921 caaaatcttc aggctcccat tttgtaatat tttttgccat tatctaaaat cctctacatt
     7981 taagccaaat ctatttaaca atgcattcat agaaagccac ctgccttctt ttgtaggata
     8041 attacaatct tcttgtcccc atatccattt atatactttt aatattgttt caataggaat
     8101 tccgaccgta tctccaaggc ataaatctgc tataacatca tcaggttcta aaccacaaca
     8161 cactttataa agtcctttta tcactaacaa cgcatggttt tggttataat catatttttt
     8221 ggcgatatca tcgctaattt caccgtgact tattaaccat aaactttgtt cgtgtggatt
     8281 atagataaag accataaaat catctcttga taacctacca aaatttcttt tgcctcccgg
     8341 cttatttata gcgattattt caccatttgc taaattctca accttataca atagatacgg
     8401 tatattattt agctcatccc taaaagcatt ttgcttttgg ttttcaattc gtaacggaac
     8461 taaattcaca ttgtatttaa attcattctt tgacattgat acctcaaatc attatttctt
     8521 cgattattcc gtcttccaag tccttgatat tgtatatgtg ttccattgca tcaaaagttt
     8581 cctcaagatt gtttctagcg cttatccaac ctttgccatc tgtaaaccac acaaaagtaa
     8641 aacctgtaac aactgcggtt tccattgcaa gatttttgta actacgagca gtttcattga
     8701 gtttacttcc actactgcta taaaaattgg tttctatacc gtagaccatt gtaggtgttt
     8761 ttgcaacaaa atcaaaacgc ttttccattt ttccctgatt ggaaatggca gaaaggttaa
     8821 ctccccattt ctcactaatc tgttgaatat acatttcttt gaaataattt atatctttaa
     8881 caaatccggc ctttttgata aagctttcaa ctaaattttc cattaggtgc ccgccacggt
     8941 tttttcgacc gttggaatcc aatcctgttt ctactcctgt ggcataatca atcaaattgt
     9001 ttacaacatg tttttttaat aaatcaaaaa gtccggtttt tttcataaag tatttatatc
     9061 tttcgtattg agtaggattt tttagctgaa tattctctgg atcaaactgg aataaataac
     9121 cgccattttc gtcctggcaa tatatttcgt ttgctctaac agctaaaagt aaaggaatac
     9181 acttcaagac ttcaggatac tttttaatta aattctcaaa atccacttca atatttttcg
     9241 agccgataag agaatttagg atattaagtt ccactttaat attatctata tttttatgga
     9301 ctttcttaaa atctatgtag taaccatagt cagcgatact attacgaaaa ctcaacaacc
     9361 atgcattaaa atctctatct gccatataca atctccctta aaaatttgat ataagtaact
     9421 cttttatctt acctcttgct tcactgttgc agttaatcat tcgtgttgct tcaactcttt
     9481 tgatattatg agatgagtaa atattatcaa agaaattatc ttctgtatcg gagtttttag
     9541 gatcggaatt actgacaaca attttagcac cctttcgatg catagcgtca acaaatttag
     9601 caagttcaat ctgttcccca tcattgaaca gattttccgt atatgcggtg aagtttgcag
     9661 tatcggttag agggcgatat ggagggtcga tgtaaacaaa agtgtttttg tcaatgaaat
     9721 ttgcggactc tctataatct ttacatacaa tcgttacatt ttgcagtttt tcggaaacag
     9781 catgaagatt ttcccggtca catatcaagg ggttcttgta agaacctatg ggaacattaa
     9841 acataccttt cctgttaaca cggaacaagc cgttgaaaca ggttttatta aggaaaatca
     9901 taagagcagc tttctccaca ttgacatttt cgtttccgtt gagcttcaaa tggttgaaac
     9961 ggaatctttt ctctaaatag tactttttac gtgaatctgt gctcatcggc acaaattcct
    10021 tttgcataac ggtcagcata tctactaatt catcaatatc atccctgata atacgatagg
    10081 tattgataag ttccgcatta atatcactga tatataccgc ttctaaattg tatttgctta
    10141 atatatcaaa taataccgct ccgccaccga caaaaggttc cgcatacttt gttattttac
    10201 ctttcttaaa cggatagtac ttttcaattt cggaaagcaa ctgccctttg ccgccggccc
    10261 atttcaaaaa aggcttaatt ttcttttcat tattttttgt gtaacgaata cttcttgcat
    10321 caatcggttt tttagcggta acaggaataa tccacatatt tccgaccata gctacattgt
    10381 caattctgtc ttcggaacaa agatatgcaa cgcgcctttg agaaatgccc cacttatcag
    10441 cagcttcttt tgcagacata aattccataa ggaaacctcc atatactaac agttatatat
    10501 tattccatat ctcgaacaat agcaatatca ttttatgcaa tgagtgagac tttttcggga
    10561 cagattaata ttgtatatcc gttatgttat actaaacggg aaaggacagt attttaatgc
    10621 aatcggaaag acttgactat gccttgaata tgcttttgga gggtgaagat ataaatcttc
    10681 tgaaaaagca tgccgaacgg ataagcgaag attacagaga cggtacaggt ccgagtaagc
    10741 gtatagtgaa tgatgcaaaa aaagctaaag catatgctgc atccagaatg cccgcaacat
    10801 accgggcggt atatgacagc ttaaggtatg ttatttccgt atcggacctt tgtgtaagtt
    10861 ccgttctgga tgtcggagcg ggaactgggg ccggtatatt tgcatgcacc gaccttttgc
    10921 aggcagagga tattacactg ctggaaagag aagagagtat gataagcata ggaaagcagc
    10981 ttttttcctg cgattccctg ctttcttcag cccgatggat taaagcgtct gtgcaggata
    11041 taggtacgga taagaagtat gatcttgtaa ccgcatcata tgtaatgaat gaactgaatg
    11101 aggaagatat gttaagaacg gctgagaaat tatgggatat aacaggtgat actctgctga
    11161 tagtggagcc gggtactcct aagggatatg caaatattct ggttataagg gatattcttt
    11221 tgcaaaaggg agcatatatt accgctccgt gcactcatgc cggcaagtgc cctatggttg
    11281 aggatgactg gtgccatttt tattgtcggg tgcaaagaag caggcttcat aagcaactta
    11341 aaggcggaga agctcctttt gaagatgaaa aatactcatt catggctttt tcgaaaaaag
    11401 aagcacagtt gcctgaattc agggtgctga gacgtcctag gatttacaaa gggcatataa
    11461 tgctgacagt atgttcaaaa gaaggtatac aaaatatcac cgttacaaag aaagaaaaag
    11521 aaagatacag agatgccggg aagcttaaag cgggagatgc tttttattga ttaggttttg
    11581 tgttcatgac tatatctttc agtcttgaca tgaagtatct gtagttttca aaagttacgt
    11641 tacttaatac catatggtcg ggaccgggta tgaacctgcc cagctttata agtgcctcgg
    11701 ctttttccag ctcttcatcc attgcttttt tgccgtaagc catacagtct ttgttaagtc
    11761 cgccgattcc tcccatgccg ggcagttttt tccggacatt tgcggggttg caaccggcca
    11821 atgattcgaa cggatatagt gcgttaacgc ccgctgcata catccattcg gcaagaaggt
    11881 cggtattgcc gtcactgtcc acaagcagta tgggaatatc gtgtgcatcg gcaaaagccc
    11941 ttatggttct gtaatggggc gctaaaaact cgttgaagtg ttcgggagat ataatggaac
    12001 cgcatttgta ggccatgtcc tcccagcttt ctataagatc gaatcgtatt ttgtcgcaaa
    12061 gtctttccca cagttccagc agcatattaa tatattcggt aataatttct tttaccgtat
    12121 cggggctgtc gtaaaacata tatccgagat tctcatcgcc ggcaagcctt cggataaatc
    12181 cgtaaacacc tccgcaggta agctgaagcg gataatctct gtttttgtaa atttcggcaa
    12241 gttcatctat atcccgaggt attctttttt ccacatcaca tacgacatac ggtcttatct
    12301 tttcccagtc atctgcggtt ttcaaagcgt actctataac tccgctatga taatcactgt
    12361 ctttagacca tgtccttatt tcacctatac cgtttctata aatccgtacc gaacccctgt
    12421 cttcaataca tatatcctca ggcagtccgc agagattgta gtttgccgga actattgccc
    12481 agcctctgta tgttccctca aaccctataa gttgatccat atatcttgat aggttgggtc
    12541 gtttttccag ccacgaagga agcgcatggt ttgtcccgcc gaagaccctc ataaaaggga
    12601 ccctgtccac ttcctgaccg gtaagagtcc tgacaaagcg aagacgtgtg tttaactgtg
    12661 ctgccatatc aaacctcaca tagattgatt acaataaatg aatggtatca taccggatat
    12721 aacacttcaa cacatattgt ttctttcaat agagacttat taatggtaaa atatcctgga
    12781 cggtaagtat aaagaggttt gtaatgtcat taaaaagcaa caatatagcg gataatctga
    12841 atgaagaaca acttaaagcg gttacccgta cagaaggggc ggttctggtg ctggcgggag
    12901 cgggttccgg aaagacaaga gtgctgacaa gaaggatagc atatattctc gagcagggtc
    12961 ttgcccgtcc gagtcagata ctggccatta cctttacaaa caaagccgca gaccagatta
    13021 agcaaagggt atatgctcta tgtgaagaag ataagaagat atatttcccc tggataggca
    13081 caattcattc ctgttgctta aaaatgttaa ggcaatatcc cgaggatgca ggttacagaa
    13141 acaactttct catatacgat acagatgata aaaagagtgt tattaaaaac tgcatgaaac
    13201 agctcaatat ctccgataaa gcttttccgg tcgcttatgt cgcttcggag ataagcagag
    13261 ccaaggatga tcttttagga tataaagaat atgcggctgc aaacgcaaac gatttccaga
    13321 aaaaagcggt tgccgggata tatgaacttt atcagattaa gatgaaagag tacaatgcca
    13381 tggatttcga tgacataata ctcaacacat attttcttct gaaagataac aggcatatat
    13441 tggagtactt ccagaaaatt ttcaaatata tactgataga tgaatttcag gataccaaca
    13501 tagcccaata caggattttt gcaatgcttt ccatgaaaca taagaatctt tttgtggtgg
    13561 gcgatgacga ccagtccatt tacggtttca gaggagctaa tgtaaagaat atactgggtt
    13621 ttgaaaagga ttttcccgat tgcacggtta taaagctgga aaccaattac cgctcctatg
    13681 caaacatatt gacagccgcc aatgccgtta ttaaaaacaa tgtcgcaaga aagagtaaaa
    13741 cccttaagac aagcaaacct gagggcagca ggataaacgt gtataaggca tataatgagc
    13801 tgaatgaagc cgtatatgtt gcaaacgaaa taaaaaaact tatggaaaag ggatattcct
    13861 gtaaagactt cgcggtactg tacaggacaa atgcccagtc ccgggtattt gaaaggattt
    13921 ttcgcgaaag gggtttaaac tacaggatat tcggaggttt gagcttcttt tccagacagg
    13981 aagtaaaaga tgtgctcgca tatatgagag tggttttcag taccgacgat gatgcgtcgc
    14041 tgtttagaat aataaatcag cccaaaagag gtatcggcga taccaccgta aaaagaatac
    14101 agtcaatagc cgcccgtgaa tatctttctt cctatgagat tgttaaaaat gcagacagat
    14161 atgagcagct gtcttcggca gcggaaaaac ttaaggcttt cagcgatatg attgagcagt
    14221 tgaaagaata taatgtgccg ggacaattgg agcagcttta cgacaacatt ctgaaactta
    14281 ccggaataca tgaatactat atatcggagg atgttaattt cggcagagaa agagtggaga
    14341 atataaaaga gttgaagagt gctattgtta cgatgaatca atcacagcag gaagatttcg
    14401 gagaactgct gtctttggag acttttctgg aaaatgtcac attggctacc gatgccgaca
    14461 aggaagaaga atcggacgac tatatttcca taatgactct gcattcggca aaaggactgg
    14521 agttcagggt tgtatttctg gtaggtatgg aagaagggtt gttcccgtca aatttgtcca
    14581 tgagcgaggg tgcccacggc ttagaggaag agagaaggct gtgttatgta ggaataacaa
    14641 gggctatgga tatactgtat tgcatacatg ccgaggagag aaccagatac ggcaagaccg
    14701 aaccgtgcga acaatcacgt tttctgaagg aaatacccaa agaactcata aacagcggta
    14761 aacgcgcttt ctcaagaaga gaaaagagcg gttcctcctt tgattggttt acttcatcgc
    14821 aaccggtgtc ggtgacaaaa ggcgggaaac ctgtgaatat tccgggcctg aaaacttcga
    14881 cggaagcttc cgtgaagaaa caggattaca gcatcggcga cagggtaatg cacaagaaat
    14941 acggggaagg ttccataaag gatttatatg acgataacgg aatggatata gtggaagtga
    15001 atttcgatat ggccggagta aagagaatgg tactggaatt tgccaagctg gtgaaaattc
    15061 agtgattctc aatatctcat atcttaagta cattgcgatt aataccgtta tggaatataa
    15121 tttaaaaaga tgttatttaa gagtttttag atatttgtac gaaaggatat acataaaatg
    15181 aaatgcccaa attgcggaca tgaggaagac aaggttatcg attcaagacc gtcggaagag
    15241 ggttatgcca taagaaggag acgtgaatgc ctgaattgca aagaacgttt tacaacttat
    15301 gaaaacattg aaagaaaacc cataatcgta gtaaaaaaag accagtcaag acagaacttc
    15361 gataaagcaa aactgcttaa cagattgatg atagcatgtg ccaaacgtcc cgtttccatc
    15421 aacaagctgg aagacattgt aaatgccatg gaactggatt ttcataagcg tcatttaagg
    15481 gaagtgacat ccagagaaat aggcgataaa gtactggaac accttattga catagactat
    15541 gtggcatatg tgcggtttgc atcggtgtat aaagattttg acgatatgga cacttttata
    15601 aaagaaataa acctggttaa ggaaaaacat aaaaagcagg agacggaata aatgacacaa
    15661 gggaaaaagg atgtagagca gattaccgga agagacatag attttgcaaa gtggtatacc
    15721 gatgtagtaa caaaagccga actcatagac tactcgcaga ttaaagggtg tgtggtgttg
    15781 cggccctatg cttatgcatt atgggaaaat atacagaaaa tactggatga catgtttaaa
    15841 aaaacaggtc atgaaaatgt atatatgcct atgtttatac ccgagtcgct cttacagaaa
    15901 gaaaaagatc atgtggaagg atttgccccc gaggtggcat gggtgacttt gggaggttcg
    15961 gaggagctta ccgaaagaat atgtgtacga cctacttccg agacgctttt ctgtgaccat
    16021 tacaagaata ttataaaatc gtggagagat ctgcctaaaa aatataacca gtggtgttcg
    16081 gtggttcgtt gggagaaaac caccagacct tttttaagac acagagaatt tttgtggcag
    16141 gaaggtcaca ctatacatgc tacggttgat gaggccagag aagaaactca tcggatgctg
    16201 gatatatatg cggatttctg tgaaaattat ctggcaatgc cggtagtgaa agggcagaaa
    16261 accgagaaag aaaagtttgc gggtgccgaa gaaacctaca cgatagagtg catgatgcat
    16321 gatagaaaag cattgcaaag cgccaccagc cactatttcg gcgacggctt tgcaaaagcg
    16381 tttgatatca ggtttaccga taaggacaat actttaaaaa atcctttcca gacatcatgg
    16441 ggaatgtcca ccagaataat aggcggcatt ataatgactc acggcgatga ttcagggctg
    16501 aaattgcctc ctaaggtagc accggttcag gttatggtaa taccgattgc ccaacataaa
    16561 gaaggtgttt cggaagctgc cgatgcgttg ctggaaaaac tgaaactttc cggattaaga
    16621 gcaaaaaccg atgtgtccga tcagtctccg ggctggaagt ttgccgaata tgagatgaag
    16681 ggtgtaccct taagactgga gataggtcct aaagatatac agaacaatca gtgtattgca
    16741 gtcagaagag atacaaaaga aaagattgcg ttaagcctta ccgatataga taaatcggta
    16801 aaggagcttc tggacaagat acagaaaaat atgtttgaag aagcaaaagc caatctggac
    16861 aataacactt ttgttgcata ctccgtagaa gagatgagag atataataca gacaaaaggc
    16921 ggatttgtta aagccatgtg gtgcaaagat ttatcgtgcg agctggaact taaagaaaaa
    16981 gccttggtct cttccagatg tatacctttt gtgcaggaaa aactgtcaga ctgctgtgta
    17041 tgctgcggta agcctgcaaa acacatggtt gtctgggctg tggcatacta aaatctgatg
    17101 aaaagtgtaa cggtagctgc cgtaatactg gataatacaa caagaacatt tgacaaggta
    17161 tataactatc ttgtacctga agatatgtac atgtatcttg agaccggtat gcgcattttg
    17221 gtgcctttcg gaagagccga caccaagaag gtcgcatatt ttctgaactt cgtgccctat
    17281 accgaaaaac tgaaagacat aaaaatgaag tacatagcgg aaatattaga cgatgaagcg
    17341 gtactttcaa aaaacatgtt cattcttgcc aaacagataa gagaccaata tttctgcaca
    17401 tacggacagg tattaagtgc tatggttcct ttgggcttaa aggtatcggt ggaaaaacta
    17461 atactgcagg aagacggaac gcttaccctg tttaatacat atctgcattc gctggagggc
    17521 ggtataaagg aacttaacag gttacttaaa gaagacagaa ttaagattgt cgagcagggt
    17581 gtgcgcaaca tcaaaaagaa gtttgagaaa gcggcaaggc ttaagataac ttccgagcag
    17641 gtggcgaatc tcatatacgg ggatgttata aaaaatgaaa gacacttaag ggtactggac
    17701 ttattatcaa cttatgaaga tataacggtt aatgattttt cctgctatga gaacatttcc
    17761 cgaaacattc tgtcaacgtt ggcaaaaaaa ggatatatag aacttttcga taaaccctta
    17821 aaatctgaca ctgtttccga agaaattgac gaaatacaac cgagtgataa aatacctacc
    17881 gccgagcaag catatgtgac cgataccgtt tctttgtcga tagaaaaggg catatatgac
    17941 aaatacctta tacacggcgt aacgggcagc ggcaaaaccg aagtgtatct gaatctggcc
    18001 gagaaagttg taaacaaagg taaggatgtt attgtattgg ttccggaaat agcccttact
    18061 cccatgatgg tcagacgctt cacacagcgg ttcggaaaca atattgaagt tatgcacagt
    18121 cgtctttcca tgaaagaacg atataatgcg tggatgaaga taaaaagagg cgaagtcaaa
    18181 atagctttgg gaaccagaag ttgtatattt gcaccttttg acaacctcgg ccttattgtt
    18241 atagacgagg agcatgacag ttcctatata tccgacagaa cgcccaagta tcataccaat
    18301 tttgttgcag ataaacgggc gcagatggaa aaggccgttt tggtattagg ttcggcaaca
    18361 cctaaaataa ctacgtataa agtgtttgaa aaagtcggaa aagtgcttac catgaccaac
    18421 agggcatcgg gtaagttgcc ggaagtcacg cttgtggaca tgcgggaaga tgccataaac
    18481 ggaaagctgt cagtgttcag tacaacttta ctgaatgaaa taaagaaaaa tctggacaat
    18541 aaagagcaga ccatactgtt tctcaacaga cggggatatt cctcaataca gctatgcaca
    18601 tcctgcaaag ccgttgtgaa atgtcagcat tgcgatgtta ccttaacata ccataagggg
    18661 aaaaataacc tcatatgcca ttattgcggc cacatgcaga aaaagagcaa catatgtccc
    18721 gaatgtcatg aagaggctat tatggaatac ggcaccggta cggaaaaatg cgaagaacag
    18781 ttgcatgaat tgtttcccga agcgaccata atcaggatgg atcaggatac caccggtatg
    18841 aagaactccc ataaggagat attggaacgt tttgagaaag aaaagataga tattctgata
    18901 ggcactcaga tggtagcaaa gggccatgat tacccgctgg taacgctggc tggaatctta
    18961 aatgccgatg ctctgggatg cggagccttt tacgaatcca atgaaaacgc ctaccagctc
    19021 ataactcagt cgtcgggcag agcggggaga gccgacttag aaggaagggc gataattcag
    19081 gcatataatg tggactcgcc tttaataacc ggtgctatca ggcaggatta caagtccttt
    19141 tacttgtcgg aacttaaagt tcgtaaagtt ttgtcccttc ccccttatac caatatggct
    19201 ttcttaatct tttcttcctc cgagagacat ttaagtgaag aatatgccga tatagcctac
    19261 agactgttaa gcaaaacatc catgacggta tatgctcctt ccaaagccgg gattgcaaag
    19321 ctgcatgata agataagaac cagagttata gtcaaacaca aagacacaaa agagtttatg
    19381 gagaatataa ccaaggcata tctgttcatg ataaagaaac ttcccggtaa agtgaccatg
    19441 agcattgatg tttttaaaga tgaatttgat gttttataac attcaaacac cgtttgatgt
    19501 ataataaatc agtatgaata attaaagaaa aggaaggtgt catatgctga cagatatcga
    19561 aattgctcaa aatgcggcaa taaaacccat tactaaaatt gcaaagtcca taggaatcaa
    19621 agaagaaagc ttagagttgt acggaccgta taaggcgaaa gtgaaaagtt ccgctttagc
    19681 cggaaaagag caagacggta agcttatact tgtcacggcc attacgccca cacctgccgg
    19741 tgagggaaaa accacaacca gtatagggct tgccgacgca ttaaggaaaa tagggaaaaa
    19801 gtccatgctg gctttaagag aaccttcttt aggtccggta ttcggagtaa agggaggtgc
    19861 cgcaggggga ggatatgctc aggttattcc catggaagat attaatcttc attttaccgg
    19921 tgacattcat gcaataaccg ctgcaaacaa ccttttagcg gcactggttg ataatcatat
    19981 ttatcaggga aataaactta aaatagatcc gaggcttgta acctggaaaa gatgtctgga
    20041 tatgaatgac aggcagttga gatatacaat tgacggtctc ggaggcaaat ccaacggaat
    20101 gcccagacag gacggttttg atatatcggt agcaagcgag attatggcga tactttgctt
    20161 aagcgacagt atagaagatt taaaagagcg gttaggcaga attataatcg gatatgattt
    20221 tgaaggagag ccggtaacgg catcaatgct caatgcgcaa ggggctcttg cggtgcttct
    20281 taaagatgca ataaagccta atcttgtaca gactcttgaa ggcacaccgg cattcgtgca
    20341 cggaggacct tttgccaata tagcccacgg ctgtaacagt atcatggcca ctaagtatgc
    20401 attgagatta agcgactatc tggtaaccga agcgggattc ggtgcggatt taggtgcgga
    20461 aaagttctgt gacattaaat gcagaattac cggactgaaa cccgatgcgg tggttattgt
    20521 ggctacggta aaagctttga agtatcacgg cggcgtaaac aaaaaagaat tgggaattcc
    20581 caatgcggaa gcggtggaaa aaggtatgcc caatctttac aaacacattg agaatataac
    20641 cgaagtattc aaggttcccg cagtagtggc aattaataag ttctccaaag acactcagga
    20701 agaaattgat gtcataaaaa aaggatgtgc atcggttaaa gtaaaagcgg tggaatgtct
    20761 ggtatgggag aaaggcggag aaggcggaat cgaactggca aaagaagtgg taagactttg
    20821 cagaaagcgg agcaggtttg agtactgcta tgacagtgac ctttccataa cggaaaagat
    20881 taaggccgtt gccacaaaaa tatacggagc ggacggtgtg gaattcacat cggaagccaa
    20941 agccgtgctt aagataatca acagcaaagg ctataacaat cttccggtat gtatagcaaa
    21001 aacgcaattt tccttttccg ataatgtaaa actgatcaac aggcctaaga actttactat
    21061 caccgtgcgt gatttacgta tatcagcggg agcgggattt atcgtagctc ttacgggaga
    21121 gataatgaca atgccgggac tgcctaaggt gcctgcagcc gaagccatag atattgataa
    21181 tgagggtaaa ataacgggat tgttctaaat aatacattaa ctgtgctatc atatccttaa
    21241 ggggggtata ttatggcgga tttaatgatg aaaagaatac cggcatccga ttggcaggac
    21301 gcattacctt tgggaaacgg ttatgtaggt gccatggttt acggcaacat acaaaaagaa
    21361 aagatattac tcaatcacga aaggcttttc cgctatcaga agcaaccgga ttatgagcct
    21421 ttctacaagt atcttcctca tgtcaggaaa cttatgctgg aagagaagta ctccgagggt
    21481 gaaaaatatt tcaatgaaaa atttaaagag tgctgcaaag aggtctataa cgtagatcct
    21541 tatcagcctt tttgcgagat ctgtctgtac agaaaacccg atgaaacttt taccgattac
    21601 agcgcaacac tggattttgt ctccgccgtt gcgaccgttc ggtggaagga aaaggataca
    21661 gagataatac gtgaaaactt tgtgtcatac gataccggat gcatatgtac gagtatgttc
    21721 tcttcggata acagaccttt agacctgaag ataaccgtag attcggttgc gggaggagag
    21781 aattatgtgg acagtttcac ctgtattcaa gacaactgtc tcgtgttcaa ttcacagtat
    21841 aaagacggca gaaagcacgg agcggtgatt tttgttaata cagataaagg cagcgtggaa
    21901 tatgacgata agttcatatc cgtcaaggca gccggaagaa tatatctgtt tgtaatgctg
    21961 tacatatatg aggatgagaa aaaagcaata aacaggctta tcaaaaccgt caagcaatat
    22021 gattacagtc aactgaaaaa agaccatatt accgtttgga ataaacttta taacaccgca
    22081 agccttacgt taggtgacga aacgaagctt atgtccaacg aggagattct tttagaggca
    22141 tatcagaaca aaaagcctgc agacctgttt cacaccatgt ttaattacgg taaatacctg
    22201 tacatatcat catcaagaga aaacggatta cctgttaatc tgcaaggcat ctggaacggt
    22261 tcatatgagc ccaaatggga ttcggatata cataacgacg agaatgttca gatgtgttac
    22321 tgggcttcac tgcccttggg gttgtttgag tcttcattgg cactgtatga ctattttgat
    22381 tcctttattc cgcatttcaa ggaaaacgca aagaagatat ataactgcag agggattctt
    22441 gttcccatat gcatgagtac tcacggaaaa atatgcggca catcgggaaa atggaactcg
    22501 tggatagctg cgggaggctg gattgccgct cactattacg attattggct ttttacggaa
    22561 gatgaagaat ttctgaagca cagagcggtt ccgtttatga agcaggtggt gctgttctat
    22621 gaagatttcc tcatcacagg cgacaacgga aagtatatct ttgttccgtc tatctctccg
    22681 gaaaacgttc cgatacatga aaatcgctcc atgatgtgcg taaatgccac catggatgtg
    22741 gcaattgcga aagaatgtat aaccaatctc ataaccgcat gtaagcatct gggcattgaa
    22801 cgggaaaaca tttccaaatg ggaggccatg ctttcaaaaa tgccggaata tgagataaat
    22861 gaggacggag ccatgaagga atggttgtat gacggtctta aagataacta ttaccacaga
    22921 caccaatcgc atatatatcc gctgtttccg ggaaatgaaa ttacaaaaga aaacaatcct
    22981 aagatatatg atgccttaaa aaccgctgtg gacaaaaggc tgctcacggg atttgtacag
    23041 caaaccggct ggtcttatgc acatatggca aatatctatg caagactgga agaccaagaa
    23101 agcgcactgg catgcttaaa ctatatattg agatcgtgca ccggagccaa tctttttacc
    23161 tatcataacg actggagaga ccaggggctt acaatgtatc cgttcggtaa aaatcctccg
    23221 ttccagattg atgccaattt aggatttacg gctgccatga ttgagatgct catattctcc
    23281 aagccgggat ttatacgcat cttgccggca ttgcctgacg attggaataa agggaaggtc
    23341 aaaggactgc gtaccagatg cggctgtatt gcggatatcg agtgggattt gaaaaagaag
    23401 catataacgg tgaaactggc ttcattgaag aaagaacctg ttgagataga gataaccgcg
    23461 ggctttaaaa agattgaatg taaagagggt actctgttat caaaaagaga taacagatat
    23521 ttaaccgtgc aatctgataa aggaaaacag gttattttgg aaatcggcat ataacaaggg
    23581 tgaaaaaagc aaaccgttgt tgtataattt tattggagat aaaaaaatga aagataaagg
    23641 atttattatt aaacagatgg aatacttcgc cgagtacaga gacagggaag tattcgggat
    23701 gacgccttat gaaatactgg agtataaact tttcactttg ggttactacc atgaccaaaa
    23761 aggttttgag gtgccggagg atttattcta ctgctcttac gaacttattg atcagtacat
    23821 gaataaaaaa ggtaaagtcg gatatatgcg tgtgtatagt cctttggaat attaccgtgc
    23881 atgtgaggat ataaagacgg aaataaatat atcgcatata gaaaacgggg tgcgcattct
    23941 gtcggtaacc gaaacatata tcgattattc agcaaaaatt gaacccgaag ccgtgatata
    24001 tcccaactgc tttattgaag gcgacagttt cataagtgcc ggaagtttaa taggaccgga
    24061 cagcaagatt gtgtcttcca agataggcgg gaacacaacg gttttgaaat ccgttgtgct
    24121 gcaaagcacc gtaggtaaga attgttcaat aggtcctttt gcatatattc gccccgatac
    24181 aacagttaag gataatgtaa aagccggaac atttgtagaa ctgaaaaaaa cagttataga
    24241 tgacaatacg acaataccgc attttgttta tgcgggagat gccgagatag gcaggcattg
    24301 caatatttcc tgcggtgtca ttaccgctaa ttatgacggc aaacaaaagc ataaaacaaa
    24361 gataggcaat aatgtgtttg tagggtgtaa cagtacttta gttgcaccgg tggaattgca
    24421 ggataatact tttgttgcgg caggttctac tgttgtggaa gatgtaaagt ccggttcgct
    24481 ggcaattgca agagcacaac aggtaaacaa agaaggctgg atggaaaaga ccggcaggat
    24541 gaaaaaggaa tagatagggg gaaggtatat tggccagaaa tgatattaaa attatgggag
    24601 gcaacgctaa ccttaagctt gctgcggaaa tagcaaacta tctcagagtg ccgcttttgg
    24661 attcaaaagt gggtagattt gccgacggag aaattcaggt tatgataaac gaactggtaa
    24721 ggggatcgga tgttttcata attcagtcca cttgccctcc ggttaatgaa aatctgatgg
    24781 aactgctggt aatgaccgat gctttaaaac gctcatcatg tgccagagta acagccgtca
    24841 tgccgtattt cggttacgca agacaggaca gaaaggctcg tgcgagagat cccatttccg
    24901 caaagcttgt agcaaactta cttaccaccg caggtgtaga caggatactg actatggact
    24961 tgcatgcacc tcagattcag ggatttttcg atatacccgt agatcatctt gaaggcttga
    25021 taatattctc cgatatgatt gcggatatta tgaaagggca agataagtcg gatttcatat
    25081 tggttgcacc ggatatgggc tcggtaagca gaataaggaa gatggcggat ttaaccgacc
    25141 ttcagatagc cattatcgat aaaagaagag aaaaagccaa tgaatgcgaa gtgatgaata
    25201 taatcgggga tatcgaaggt aagaccgcga ttataattga cgacatgata gataccgcag
    25261 gtaccataac caaagccgca aacaccgttg tggatatggg agcaaaaaga gtattggtat
    25321 atgctacaca cggaatatta tctgcaaatg ccgtatcgaa gattaagaat tccaagattg
    25381 agaaaatgta tcttctggat acggtacccg tacctgaaga aaagatgata gacaagttgg
    25441 agatagtgaa tgttgcaaaa ttgttttccg aggctatcta caagatatat acaaacacct
    25501 ccatgtccgc tcttttcaaa atgccgccga gcggtagaaa tgaaaagtaa tggacagata
    25561 tttaatagcg gggctgggaa atatagggct cagatacaga aacacaagac ataatgtagg
    25621 ttttatggcg gttgacaaat tagccgatgt ttattctatc aaagttaaaa aaaatctctg
    25681 tcaggccaat gtcggggaag gctttatcaa taatattccc gttattcttg ccaaacctaa
    25741 gacttacatg aacttgagcg gtcagagtat acgcgcactt acggactggt acaagattga
    25801 tatttccaga cttattgtca tatatgacga tatggatatt cctgtgggag aacttcgtat
    25861 acggtataaa ggaagtgccg gttcgcataa cggaatgaaa tccatactga gccatctggg
    25921 taccgaccgg tttatccgta taagagtggg aataggaaag catgagcata ttgaggctgt
    25981 ggattatgtg ctgggcagat ttcccaaaag cgaaaaagag attatagaca acactttaca
    26041 ggacttgaca aaggctgtaa catgtattgt gacggaaggt acagatatag ctatgaacag
    26101 atataacaag aagaatgaca gaggtgatgt atagctaaat gaatcctttc atacaggtct
    26161 tttccgatat ccctcagctt ataggggtag cggactgcat agcgttgaaa cagacccccg
    26221 taggattgtc cggaccgggg acttctttat gcccgttaat aagccacata acagcctcat
    26281 cggtatcaag aaagctgtta atagtgactc attccagaac tgccgcttta tctgtacacc
    26341 gtaatctgat tgctttttac ggtgatgatg cggtgcatat accggccaga gagttgatgc
    26401 tttttgatat tgacgcatac agcggaaatg ataaacaggc caggcttaaa gcgttataca
    26461 gaatagcgac aggcgatttt aaggcggctg ttgcctgcac cgaagcactt acagtaggta
    26521 cggcttccag acaggtattt ttgcaaaatc tcataaatct gaaaaccggc gaacagcggg
    26581 atatggaaac attaggagat atgctgatgc gtttaggata tgtaaggaaa gctctggttg
    26641 aggaacaggg acagtacagt ataaggggcg gcatattgga tatattttcc tgcaattacg
    26701 ataacccgat aaggtgtgag tttttcggag atgagataga cagtataagg tttttcgatg
    26761 ccgattcgca gcggtctgta gaagagactg ataatgttgt cataatacct tacagcgagt
    26821 ttatcttgga ccggaatgat gagaaaatac ttaaagagaa aattaccgac tcttgggact
    26881 tgcagcagtt taacgagggt gactatgcaa agattatgga cagatattat gccgcgaccg
    26941 ataaatgtgc tatgaccggc acatatttcg ataaagacat acttacggtt atacatgacg
    27001 atttgcaatg tcattcggtg gcggataccg tatacaaaga atatgcaaac gcctgcgtca
    27061 atcttttgca aaagaaaaag accttcaagg aagcatgtga aatacagaag gatatatatt
    27121 cactgtattc ctgccttaaa ccgcaactga tacttgtata tgagaagtcg aattacagta
    27181 ttccccttta ccgggatttt gatattacgt tcgaagaaac atgttcctac aagggagata
    27241 tggagctgtt tttgaacgat atttccgagt acagagccgc caatatgcgg atattcattt
    27301 cttccgacag aaaaaaacat accgaatact taaaaggttt ctttgaagat aacggatata
    27361 cggtacatgt cattcagcct aaggaaaatc ccaccgttat tcccaggcac atatatataa
    27421 ttgaagacgg ttacggtgag agttttgtgt ccaaagaagc aaagatagcc tgtatttcgg
    27481 taagccgtat attccgcaag agtacaagag gtaaaacccg gcagataagg gatgccgatt
    27541 tctttgccga gattcaacca aatgactatg tcgtgcacga tcatcacgga ataggcatat
    27601 atcagggtat agaaaagatt gaaatagacg gaattattaa ggactatatc aagattgcat
    27661 ataaaaacaa cgacatgtta tatgtaccgg taaataaaat ggatttggtg cagaaatatg
    27721 tgggcacatc ggacagggct ccgagaatta ccggactagg cggtcgtgac tgggaaaaac
    27781 agaagagtaa agtcagaagt gccgttcgcg atattgcaaa agaactgatt gaactgtacg
    27841 gaaagagaag ttccgtaaaa gggcatgctt tcagtgaaga tactccgtgg cagatttctt
    27901 ttgaaaacga atttgaatat gaggaaacgc aggatcagat tcagtgtgtg gaagagataa
    27961 aagccgatat ggaatcgaca aaagttatgg atcgcctgtt gtgcggggat gttggctacg
    28021 gtaaaaccga agtggcttta cgtgcggcat tcaaggcgat aatggacggt aaacaggtgg
    28081 cctttcttgc tcccaccacc atgcttgctt tgcagcacta taacaacttt cgggagcgaa
    28141 tgaaaaatta tcccgtaaac atagatttac tttgcagatt gagggataaa aaaagacaga
    28201 ctgaaactgt agagtatata aaaagcggga agacggatat agttgtggga acgcacagac
    28261 tgctggaaaa ggatgtgtca tttaaagacc tcggtcttct ggtagtggac gaagaacagc
    28321 gcttcggagt ggctcagaaa gaaaagatta aaatgttaaa acccacagtg gatgtactat
    28381 cgctgaccgc aacaccaatc ccccgaacca tgcacatggc actgtccggt ataagggatg
    28441 tcagcaccat atacgatcct ccggaaaaac gttttccggt acagacatat gttatggagt
    28501 atgatccgga gttcattaag caggtaatct tacgggaaaa agaccgtaac ggacaggtct
    28561 tctatctgta taaccgcgtt atggatatgg acagcaagtt ttatcatctt aaaacactct
    28621 taaaagatca agtcaggata tgtgttgcac acggacagat gccggaaaaa gagtttgaaa
    28681 ataccataat aaagttcata aaccgagaat atgatgtttt actgtgtacc acaattattg
    28741 aggccgggat agacatatcc aatgccaaca ctctcataat agaagatgcc gacaaactgg
    28801 ggttagcaca gttatatcag attaaagggc gtgtgggcag atcggacagg gttgcctacg
    28861 catatatcac ctacaaaaga ggcaaagaac tgaccgaaga agcttccaag agattacaga
    28921 ccataaaaga atatacagag ttcggttcgg gaattaagat tgcgatgaag gatctggaga
    28981 taagaggggg aggagacctt ttaggagcaa atcagtcggg gcatatgcag ggtgtaggct
    29041 atgacatgta tgtcaggatt ctttcggaag agattgcaaa cttacgagga gaaaaagtta
    29101 aaggtgcagt taccgaaaca agtgtggatt taaatataag tgcatatatc gacaagtcct
    29161 atatatccga tgacttgtcc agacttaaga tgtataagag gatagcaacg gtcgcaacgg
    29221 ttacggaaaa ggaagacata atagacgagt tgacagaccg gtataatcag cctcccggtc
    29281 atgtcattaa tataataaat gtggcatatg caaagaatct ggcatgcaga ttggggatat
    29341 cggaaattgt acagacaaac aaaggttttg taatgatata tgaaaaagag catgctatgc
    29401 aatttaatga agtaaccgac atattggata aagaacataa taaatataag gtaaaagcgc
    29461 aggaagattt acaaataagg gtggataccg ataaaaagaa tgaggaattg ttggatttta
    29521 taacaaaaat actggaaaaa gctgttgaat caaaataagg cttgataaag agcagtcgtt
    29581 tattatagaa taggtagacg gaaaacggtt tatttacctt aaaaaaaagg agataggaaa
    29641 tggcagtcgc taaaggcaaa aaagaagatg tcaagaagaa gagcagcaag gaaatatcca
    29701 aagaagaaac aataataagg tacggatttc tgacgcttgc cgttatcatc ggcctttgtt
    29761 tgatttattt cgttatagcc agtttgatac ccaataacta tattaaaata ggcaatcata
    29821 aaattgccga agaagagttc atgtaccaga ccaatacaca gtataaagtc tttgtaaact
    29881 attatcagga ctatatcaag aatctcggat ttgatttatc aacaccggaa ggcatatcgg
    29941 ctttcatgaa gacggcatat gataatcagc gttcattcgg tcagatgttg ttggaagggg
    30001 tggtagattc catacaggaa cagtatatta tgttagacca aataaaagag acgggattta
    30061 agattgatga agaagagctg gaagaaagtt tcgcaataca gatggaagct attcagagag
    30121 cagccgatga atcgggttat gatcttgaca aatatgccaa gctattctac ggtaccacat
    30181 tcaaagcaat gcaaagagtt ataaagcaga ctttgagagc acagcaatat acaacctatc
    30241 tggaagaaca aaacatcagt gccgtaaccg aagaaagagc acgttcgtat tacaattcgg
    30301 ttgatgccga cggaaatgcc gtcagagatg atatagacaa attaacggta gttaccattt
    30361 taaaacgtac cgttgacgat gaatttcagc ctcttgccga ggatgttgta gccgcagcta
    30421 aagtaaaagc cgctgagata tttgaaatgg ccaagagcgg ccgcgatatt tatgaactgg
    30481 cgaagcttta cagtgatgac ctgaaggatc aggaaaccga agagtcggag gaagaaaccg
    30541 aggataccgc aaaaccgggc gaatttacat ttaccaagaa ggaagtagga acaaccgagt
    30601 taacacagtg ggcacttaag gcttccgtgg gagatattga tcttatagag accgagatag
    30661 gtcatgtaat aataaagctt atcgacagaa ccgattatga agacgtgaag gatgacatat
    30721 tcaagctgat tgcttccagt gattttgagg ttaaaatgaa tgaatataag gatatggagc
    30781 aatataaggt agggctttac aaaccgtatc aggaactgta tcttacttat gcgataacac
    30841 agtaatacgg taataaaaac aaaaaaagaa acctccgcag gtccgacaaa gaaaatgaac
    30901 ggataggcgg gggtttttgt ttgctttgac ttgaatatat aatttaacgg taatataatg
    30961 tatgagttaa atacttaaat ttgaggtgga tataaaatga catttttaga aagaacaatt
    31021 caaagagcca agagtaataa aaagaccata gtattgccgg aatcggaaga cataagaacg
    31081 cttaaagcgg catcggttgc actgaaagaa ggtatagccg atataattct tttaggaaat
    31141 aaagaaaaag ttaagaaaaa cggcaagggc ttgaatatcc ccgaggaaaa ggtattggaa
    31201 atagataaat tcgacagaat tgatgaatat gcaaatatgc tgtatcagct gagaaaagaa
    31261 aagggtgtta cattggatat tgcatataag cttttggaga atccgttata ctttgccgtt
    31321 atgatggtaa aaatgggcga tgcagacgga atggtggcag gtgcggtaag ctctacaagt
    31381 gataccttaa ggccggctct tcagatactg agaacggcaa aaggaaccaa gatagtcagt
    31441 gcatttttca taatgtgtgt acccgactgc caatacggag caaacggtgt gtttgcatac
    31501 ggagattccg gtctggtgga aaatcccgat gcggagcaac tttccgaaat agccatttcc
    31561 actgcaaaat cttttgaatt gcttgtggaa gaacaacctg ttgtggcaat gctcagttat
    31621 tccacatacg gaagtgcaaa atccgaattg accgagaaag ttatacaggc taccaaattg
    31681 gcaaaggaaa aagctcccga gctgctgctg gacggcgaac tgcaggtaga cgcagccgta
    31741 gtaaaagaga taggtcagag aaaagcaccg ggaagttctg ttgcgggaaa agccaatgta
    31801 atgatattcc ccaatctgga tgcgggcaat atagcatata aattaaccga aagactggca
    31861 aaggcggaag cttacggtcc tctgcttcag ggaatagcca aaccggttaa cgatttatcc
    31921 agaggctgca cggcggaaga tatagtggga gttattgcaa taactgcggt tcaggcccaa
    31981 agtgtttaat atatatcatt aaggtcaggt taacatgaaa gtattagtaa tcaatacagg
    32041 cagttcttca ttgaagtatc agatgattga cagcgaaaat gaaaaaatgc tggcaaaggg
    32101 attatgtgaa agaatagggc tacccaacgg cattcataca tatacatgtg ctgacggctc
    32161 atcaattacc gatgaatgtg taataagcga ccattcccat gcgtttaagt taatgctgga
    32221 tgctttagta tgtcccgcaa cgggagtatt gaagagcata tcggaaatag atgcggtagg
    32281 gcacagaata gtacacggcg gagagcgatt taccaagccc gctttaatag atgaggatgt
    32341 aataaaggcg atagaggact gcgtggacct ggcaccgtta cacaaccccg caggtatatt
    32401 gggaataaga gtgtgtcaga aagtattgaa aaatgtacct atggtggcgg ttttcgatac
    32461 ctcatttcat cataccatac ccgattatgc atatatgtat gctcttccgt atgaaatgta
    32521 tgagaaatac agagtgcgta aatacggatt ccacggtacc aatcacaagt atgtggctca
    32581 aagaactgcg gaattaataa acagaccgct ggataaaata aaaatagtat cctgccatct
    32641 gggaagcgga gcatccatat gtgctattga cagaggaaaa tccatagata cttccatggg
    32701 attcacaccg ctttcgggat tggctatggg aaccaggagc ggtaccatag atccgtccat
    32761 aatttccttc ataatggaaa agaagaatat gacggtggaa gaagtcaact attatttaaa
    32821 caacaaatcc ggtttattgg gagtatcggg aataagcagt gattttcggg atcttgtcaa
    32881 agctaaagat gaaggcaata aaagagctac actggcactg aacatatttg cttacagggt
    32941 taaaagatat gtatgtgaat atgtgggagt aatgggagga atagatgctt tggtatttac
    33001 cgccggtgtg ggtgaaaaca atgcatatat aagggagcgt ataaccaaga atcttgatat
    33061 attcggaata aaaatggaca gaaggaagaa taacaacgca aaaggcgaaa ccgatgtaag
    33121 cagagaccga tccaatgtaa gaatactgat aataccggca aatgaggaat tggagatagc
    33181 cagacagtgt aaaagcctgc tggaagacgg caagtaaagg aaaattgtct tgacaaggca
    33241 taagaggtaa ccatataata gcacttgtcg gttttttgaa aggtattaaa tgaagattga
    33301 cataaccgat attatagaga gagacggcgc tgtaaagcat tttgtaaccg atgtcgacaa
    33361 gttgagtgac cgcaataccg gcagtggtaa gtctattgtc gatgtacagg gtgttattca
    33421 cattgaaggt gagatttaca accatgaagg tatcctggac atttcggcgc aaatcttcgg
    33481 gcagttgaaa gcgcaatgct acagatgcct tgaagacatt actgcccctt tctctgtgca
    33541 tatggaagag acttttgaac gcgcgaacgg ttcaaaaaac ttggataact acgagtattc
    33601 cggtcacata ctggatatct cccaggcggt aatcgattcc gtatatttaa atatccccta
    33661 cagaatgttg tgcaagagcg attgtaaggg gttgtgccct aaatgcggaa gcaatcttaa
    33721 tcaaaaagaa tgcggatgca ttgaacaaag cgaagagaac gggagatttt ccgctttgaa
    33781 ggatttattt aaagaataat gttagtaatg caggaggtgt aaaatggccg tacccaagcg
    33841 taaagtatca aaacaaagac agcgtaaaag aagaactttc tataaactgg aagagcccaa
    33901 tcttgtcaga tgttcacagt gtcatactct caaacgtcaa cacagagtat gtgacgaatg
    33961 cggatattat gacggaaaag aagtaataaa aaaagaagag gaaaagtaat gttataaggc
    34021 gatgatatat cgcctttttg ctatatggaa aacaaggcaa ttgcaattaa acagatattg
    34081 aaaggaagtt tagcgaagag agccggtata aaacccggcg attttttgct ttcggtaaac
    34141 ggcttcggta taaaggatat tttcgattac cagtaccata tttccgatga atcggtgctc
    34201 ttggaaataa taaacggcaa gaccggaaag cttacgggag tggagattaa aaaagacatt
    34261 gacgaggacc tgggaatagt ctttgaaaag gaacttatag atgacgagaa agtttgccgt
    34321 aacaaatgca tattctgttt tattgatcag ttgcccaaag gtatgagaga aacgctgtac
    34381 ttcaaggatg atgatgccag gctttctttt ttgaccggaa actatgtcac attgaccaat
    34441 atgtcgtatg aagaaataaa aaggatagct tctatgcatt tatctcccat aaacatatcg
    34501 gtacacacca ccgatccgga attgagatgt acaatgctga acaacagaca tgcaggagat
    34561 attttggata aaatggctgt acttgcagaa gcgggaacag acttaaattg ccagatagta
    34621 ctgtgtaaga atgtaaacga taaggctcat ttagacagaa caataaagga tctttcatgt
    34681 ataggacagc cgcttaaaag catttccgta gtacccgcag ggcttacccg ttacagggac
    34741 gggctgtatc cgttggagcc gtttaccgaa gaagaatgta ttcagattat aaatcaggtt
    34801 caaaaacatc agaaagtatt caaagagaag tcgggatata accttgttta tgcggcggat
    34861 gaattttata taaaagccgg tctggagctt ccttctttcg gagaatatga tgatttttat
    34921 cagatagaaa acggtgtagg attgatgaca cacttcatac acgatgcaaa aaattatctt
    34981 gaagagatat caaacagcaa aaaacttctt aaaagggcgg agaaaaacac aaagcacaaa
    35041 agtatagcga ccggagtgct ggcttatcct tttataaaag aacttacgga taaagttagc
    35101 atgtacgcaa agaatatgaa aatcgatgta tatgagatta aaaacaattt tttcggagag
    35161 aatataacgg tgaccggact gctgacggga caggacattt cggaacaatt gcaaggaaaa
    35221 cagttagggg agcaactcct catctcccgt accatgctga aggcaagcga tgaatatttt
    35281 cttgatgata tgtcctacaa acaactcggt aagaaactca agacgacagt taccgttacg
    35341 ggcaataacg gagaagaatt cataaagaca cttataaagt aaataaaaca tatcaagacg
    35401 gaatgagtac aaaggtaaag taaaatattg caaaagtgtc aagtaaaaca ttgcaaaagt
    35461 gtcaagtaaa acattgcaaa agtgtcaagt gcatgatatc cttttggtat tagataagaa
    35521 gacgaggtat tgtaacgagt aaagtgaagt acagaaaaag gatagttgat gaacagctgg
    35581 atttcaaatt agaggcattc ggagccactc ttattgaggg gccgaaaggt tgcggaaaaa
    35641 ctacaactgc taagcagaaa gcaaaaagtg ttgtggaatt tcaaaatgag gatgaacgtg
    35701 aaaattatct gataattgcc aataccaaac catccgagtt attaaagggt gaaaaaccca
    35761 gactttttga tgaatggcaa gatgctccga aaatatgggg agcgatacga aaagatattg
    35821 atgatacagg cggggtagga aaatatattc taacaggttc cagttcaaac actgttaaaa
    35881 ctccgcatac cggaacactc agaatatcca ggctaaagat gtatcctatg agcctttatg
    35941 agagcggaga atccaacgga gaagtatctc ttaaagaatt gtttaccaat cccgattcat
    36001 ttgatggatg tcgctctgat ttaacaatag acgagattaa gttttccata tgcagaggag
    36061 gctggccggg aagtttggat agaagtactc ctaaaactca gttggctatt gcaaaagatt
    36121 tatttaatca aacatgtaat gtggacataa gtaatattga cggtgtaaat cggaatcctc
    36181 agtgggccaa agctatttta aaatcatatg ctcgtaatct ttgcacctta gcaacaacaa
    36241 agaccatttt agcggatgtc aatgcatcat gtgaaatgtc taaacaaagt tattatgatt
    36301 atgtatcggc tttggaaaaa cttatgatta ttgaagatat tgatgcatgg aatcctgcaa
    36361 tcagatcaaa aacatctata cgctcctcgg caaaaagaaa tttcattgat ccgtctattg
    36421 cggtggctgc tttcggtata tcgccggaat actttgacag agactacaaa acactcggct
    36481 ttttatttga atctttatgt attagagatt taatagtgta ctcctcaaac aatgacggca
    36541 atctttctta ttaccgtgac aggctcggtt tggaagcgga tgcggtttta cacctggacg
    36601 acgggcgata tgcacttata gagataaaac tgggtcagag tgatgtagat gaatcagcca
    36661 aacacttgtg tgaaattgaa actttgatta gagaatacaa tgaaagtaat aaaaaagttt
    36721 ctctcaatat gccgaccttg aaaattatac ttaccggtac gcagtacggt tatcgcagag
    36781 atgacaaagt ttttgtgata cctatagctt gtttaagaga ttgaaaattt tttatcttac
    36841 atcacattgt gttctgtgca gattatactc tgcaatgtgc aataatttta gcactatgat
    36901 aaaatatgca taaaggaggt ggttttgtgg ctgcatatac tgtttcggaa ctaaaagata
    36961 ttgtcgggaa attagccgct aaatatggag cagagcgtgt atatctcttc ggttcttatg
    37021 ctcgtggaga agcaaaacct gaaagtgata ttgatttgcg aatagacaaa ggcagtattc
    37081 gcggattggc ttttgcctct ttactcggtg atttacaaga tgctctcgga gtagaggttg
    37141 atttgatttc taccgccggt atggataacg attttttacg tgaagttcag aaagatgagg
    37201 tgctgctcta tgaaagagca tgagcgtaat gttcgcattt tatcttcaat tatttcttat
    37261 tgtgagcaaa tagatacaac cgtcaggcat ttcgggaaag aatataagat attcttaaac
    37321 aatcctatat atcacaatgc cgtctctctt tgcgtgatat taaactgatg agaaacatag
    37381 ttgctcatcg ctcccgcttt agaagcattt tgccgaaata ttcttaaaaa agaaagttaa
    37441 aacccgattg cataataaac ctaatgagta aaacggatag gtttgtttcc gcatagcttt
    37501 ttatcccggc taattccttg ccgtatctta tatgcaattc ttgagaaaac accatgtata
    37561 tggtattatt tatccagttt atttgaaagg cggagcgggg aatttggcac agccgatagt
    37621 ggccgtagtg ggcagaccta atgtaggtaa atcagcattt ttcaattatc tggcaggcag
    37681 aaggatatcc atagtggaag ataccccggg cgtgacgcgc gacagaatat acactaaatg
    37741 cgattggtgc ggtcgggagt ttgtgcttat cgataccgga ggtatagaaa tcggttcttc
    37801 ggacgaaatg tataaacaaa tgaaaagaca ggcggaagtt gccattgaca ctgccgactt
    37861 tataatcttc atgaccgata taaagagcgg acttactgcg ggggataagg aagttgcatc
    37921 actgctgcat aaatcgggaa agcctgtggt aattgcggta aataaagcgg attcgccggg
    37981 agagcctcct gcggaattat atgaattcta caacctggga cttgcggaaa tctttcccat
    38041 atcggctatg cacggactgg gaataggcga acttttagac tatgttatgg agcatcttcc
    38101 cgatattcag gagcaatcgg atgatgacag tatacatgtg gcaataatag gtaaacccaa
    38161 ctcgggcaaa tcttctttac tgaacagaat aaccggagaa gagcgtgcca ttgtcagtcc
    38221 cgttgccgga accacacgcg atgctttgga ttcattggta ataagggagg aaaagaaata
    38281 tacctttatc gatacggccg gtatcagacg aagcagcaag atattttccg acatagaaaa
    38341 gtacagtatc ataagagcct ggtcggccat tgagcgatcg gatgtatgca tacttatcgt
    38401 ggatgcatcg gaagggatta ccgaacagga ttccaagata gcgggatttg cccatgaaca
    38461 gggtaaagct gcaattgtcg cattgaacaa gtgggacctt gtggaaaaag acaacaagac
    38521 ctatcagaaa ttcgtaaatt ccgtcagggc ggagttgccg ttcatgcaat atgctccggt
    38581 catatctatt tcatcactta ccggaaaacg agtggagaat ctgtttcctc tcatagacaa
    38641 agtttatgaa aatgcaaaca gaagaatatc cacaggggtg ctgaatgatg ttctcaacga
    38701 tgcaatagct atggttcagc cgccgtcgga taaaggaaaa cttttaagaa tttactacat
    38761 gactcaggta tcgacacaac cgcctacgtt tgttatcttt gtaaacagta aggatctcat
    38821 gcacttttcc tatgaaagat atctgaccaa tacactaaga gatacgtttg attttgaagg
    38881 tacacctata aggtttataa tacgggagcg aggtaaagag aattgaatat tttactgcat
    38941 gtaatatcgg ctgttttcgg atacttggtg ggttctttga actccgccat actggtttca
    39001 cgcttttata aaaaagatat caggaatgag ggcagtaaaa atgcgggtct tacaaatgtg
    39061 ttgagagttt taggcaaaaa ggctgcgata gcggtacttt tgctggattt gcttaaagga
    39121 gttataacca ccggtttggg atatatattg gttaaagatc aactgtccgg tgcacttgta
    39181 gccggtatat ttaccgtcat aggacatata tttcccgtgt tctttaaatt caagggcgga
    39241 aaaggcatac tgatttcact ttccgtgttg ttgatgaccg actggagaat agcacttata
    39301 gggctgggga tatttataat aatcgtgggg ctgacgagat atgtttcctt aggttccatt
    39361 accgcaatgt ttctttctcc gctatttgca tggttgctta atcatgagcc tttctttatc
    39421 atatcaatgg gctttttagc cgctcttacc atatttatgc acagaagtaa tgtcaagaga
    39481 ctgctgaacg gtaccgaaaa caaactgtct ttcaacaaga ccggaaacgg ggaaggtaaa
    39541 tgagcaacct cattgttata aacacctcat tcaatccgtg gtataacctt gcactggaag
    39601 agtatctttt agataaagta accgattacg ataacatatt gtttctctgg cagaatgcca
    39661 atacggtagt aataggcaga aatcagaatg cctggagaga atgccgcact gaactgttgg
    39721 aatcggaagg cggaaaactg gcaagacggt ctacgggagg gggagcggtt tatcacgatt
    39781 cgggtaatct gaatttctct tttatacttc ccagaagcag tcaggatttt cttatacaag
    39841 caggtataat aactaaagcg gtcggtaaat tcggtataaa tgcatatgtc aacggaagaa
    39901 atgacatagt tacggatcaa ggcaaattct ccggaaatgc ttttacatac agaaaaaaca
    39961 gagccttgca tcacggaacg gtgcttgtgg atgtagaccg ggagaaattg ggcagatacc
    40021 tgcaggtcag caaagaaaaa atgcagataa aaggcattaa aagtgtgcaa agcaggattg
    40081 tcaatctcaa agaatatgac agcggcataa cggtagagcg ggtacgtgac gcgataatgc
    40141 aggcttttgt tgaggagttc ggccaatgcg agatagcgga ctgctcaaaa gagatagata
    40201 tatttgaccg caaattcgac atggcggaag tgcacagaac atatgaaata tattcctcct
    40261 gggagttcag atacggtgaa gcacccaagt ttgatataga gtgggcaaac agattttcct
    40321 ggggagaggt ggtcatccat cttacattaa aaaacggtgt tataattaaa agcaggatat
    40381 attccgatgc tttggacgaa ctgttcatag ctaatatacg tcaatgtttc gacaatgtac
    40441 cttttaagaa aaaagatatg gcagaagccg tggtgaaaca acatcccgac tcaccgatgg
    40501 caaaagatat tgcggaatat attctaagta aggagatgta atgtttttta aggatttgca
    40561 tattaaagat attgccgata taccggtttt aagtctggct tacataggcg atgccgtata
    40621 cgatctttat gttagggagc atcttttggc aaaaggatgc ttcaaaccta ccgatttgca
    40681 taaaatggcg gtcgtctatg taaatgcaaa aggtcagaaa cagagcctta tgaagataat
    40741 ggatattttg acggaaaaag aattaaactg tgtgcaaaga ggccgaaatg ccaaacccag
    40801 gagcaaacct aagaatgccg atgccgcaga ctattcctac gctaccgctc tggaggctct
    40861 tgtgggatac ctttatatca acaaagaata cgaaaggctt gattacctgt tgggtatcat
    40921 gttggatata tgatggaaga aaaaaacacc gttatagcgg gaagaaatcc cgtattggaa
    40981 gccttaaaga aggattgtct cataagccgg atactggtaa gtgtcggtga aaaagagggg
    41041 tctatactga aaatactttc cgaagcgaga aagaagaata ttccggtaaa gcaggtatcc
    41101 aataccaagc ttgactctat tattccggct catcagggtg ttgtggcata tatatccccg
    41161 atagagtttt atgaaatatc cgatcttctg gatgtggcaa aagaaagaaa agagcctccc
    41221 tttatagtaa tactggatca gattgaggat gtgcataatc tgggagcaat tgccagaagt
    41281 gccgaagctg cgggatgtca cggtattata atcccccttc acaggtcggc tcccgtaaat
    41341 gccgccgcaa tgaaagccag tgcaggtgcc ctgttgcata taaaggtggc cagagtgacc
    41401 aatctcaatg cggccataga gcagctgaag aagcaaggtt tatggatatg cggaacggat
    41461 aaggatgcaa ataagcaata ttacgacgcc gaccttaaag gaccgcttgc cgtagttata
    41521 ggttccgaag gagccggtat gtccaggtta gtagcaaaga actgcgattt taccgtaaat
    41581 atacctttat cgggaaatat aaccagcctg aatgcatcat gtgcagcggc aatagtaata
    41641 tttgaagccg taaagcaaag aaaagcgtaa aaccgatgga atacctgttt gttgacggtt
    41701 acaatataat aaacgcatgg tcggacatat ttgacaaaaa cagccccctt gaggacaaca
    41761 ggaaggcatt gtgcgatata ttgtccgatt atcaggggta tacgggtttt cggattgtag
    41821 tggtttttga tgcgcatctt gtaccggaag gaataggctc acttatgcag tatgataaaa
    41881 taaaggtggt gtttacaaag aaaaatcaga ctgcggacaa tttcattgaa agatatgtgg
    41941 caaaagagtg ttacaacaat aaaataacgg ttgtcaccaa cgactatatg gaacaaaaga
    42001 ctgtgttgca caaaggtgca ctcagaatga cggcatcgga attacaaaga gagataataa
    42061 agaaaagaca aagcgcatcg acaagcgata aacaatccaa ctttataata gacaatctgg
    42121 ataatgaaca gaaaaggaaa ctgttaaaga taaagcatga agaccgaaaa gataaatagc
    42181 attataaaaa aagccaaagc gggcgatgaa gtttccatgg aatatctttt aaaggagtat
    42241 tcgtcccttg tgcgtaaaaa agcgaccggt ctttttctga tgggagcgga caaggacgat
    42301 cttttacagg agggaatgat aggtcttttc aaagccatac tgaactacga tgaaacaaaa
    42361 gaagcttcct ttgatacatt tgcggcactg tgcataaaca gacagatgtt ttccgcaata
    42421 aaaggagcaa acagaaagaa acataaactt atcaacgaat acatttccat atacacggag
    42481 gataaagaag atataccttt agccataccg gatgagaagt ataatcccga ggtaatagtt
    42541 accgaaaccg aagcattaca caatcttgaa aacatgatac gtaataaatt gagcagtatg
    42601 gaaagtaaag ttttagatat gcttattgaa ggtcatagtg ttgcctatat gagtaaaaaa
    42661 acaggtaaaa atgcaaaatc cgtggacaat gccatacaac gtatcaaaag caaagtgcgt
    42721 aacattttga aagaagagca ttgaagtatc ggttttatat gttataatag cctagtcgct
    42781 gacatagctc agtcggtaga gcacctcatt ggtaatgagg aggtcggcag ttcgattctg
    42841 cttgtcagct ccataagaag ggtatgatat gaggtcatac cttttttttt acctgtctgt
    42901 gttgatagta acacttttta taattcaact gacaaagccg gaatctgaaa acttgatatc
    42961 gaaaaaatcg acatcaaata ggggcgaacg atagacatgt tcaagtaaca ttttgcgtct
    43021 tgaacagagg aataagaaag caggcacttt accttatacg acggaaattt accgaatacg
    43081 acgggaatat tgcatataaa aagatataca tttataatga aaacagccat gaaaggaaag
    43141 gggcaatagg tttgtgagtg taaaatttcg ctttattcat gacgatgcac tgggtaatga
    43201 tatagaaatt accgtacgtg ccgcggaaaa aaatgaacga gtggaaaagc tgttatcaaa
    43261 acttgcggag agttccggcc aacttatatc cggtcagatt ctttcggaaa agtacgatat
    43321 agacacttcc gatatcattt tggttatgcg agacggtcgt tatgttactg cgaaaacagt
    43381 cagaggagac tatattataa aagatgcact gacacgcgtg gaagaaagcc tcaaccccac
    43441 atggttcgta agaatatcgc agtcggagat aataaacctt aagtatctta agaactggga
    43501 tttcgttggc ggaggaataa ttcagataaa gatggagaat ggaataataa ctcactcatc
    43561 gaggcgatat gctacacaaa ttcgcgatat gcttcttaag aggagggcaa aacgatgaaa
    43621 aaacgtttga ttatcagatc tcttatcgga cttgttgtcg gcgcactggc agtacatgca
    43681 attactctgt ttgttaatta catcagcaga ggtgaaattt taatctgtat gccggaactt
    43741 atagaaaagc tcggacttac caaagccata attgttcaga ccgtactcgg tgcgctgatc
    43801 agcatgatag ccatcggcgg aacggttctg tttgacatag aaacatggag tttactgcgt
    43861 gccactgtgg cgcactgtgc acttatactg ttttcatatt tgacggtagg attattgttg
    43921 agttggtttt ctttccacat aattcctatc ctcataatga cgggcggaat aataattgtc
    43981 tatgcattga tatggctcat aatgtacgct atctggaaag cggaaatacg aaacatgaac
    44041 cgccttacgg aagagtacaa aaaagatata gaagcaagcg aagatcaatc ctcataaaaa
    44101 gtaaagcata taacaaaagg tcggccggaa gggtcgattt ttttgagcaa attttcagtg
    44161 aaactacact ttatcactgt tttttaaccg tttatgacgg gaaattaccg aatacgacca
    44221 aaccgttgaa tgtagaaaaa acaaccgata tactcgagtc agatctcaac gatttttta
//
LOCUS       DAOSOM010000017        42289 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_964_length_42289_cov_6.734243, whole genome shotgun sequence.
ACCESSION   DAOSOM010000017 DAOSOM010000000
VERSION     DAOSOM010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 42289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42289
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_964_length_42289_cov_6.734243"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..891
                     /gene="thyA"
                     /locus_tag="PLF14_05425"
     CDS             61..891
                     /gene="thyA"
                     /locus_tag="PLF14_05425"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOT70769.1"
                     /translation="MSRADDIFKNMCRDIIDNGFWDTGLDVRPRWLDGTPAHSVKKFC
                     VVNRYDLSKEFPILTLRPTRFKGAIDEMLWIWQKKSNNIKDLNSKIWDAWADSEGSIG
                     KAYGYQLSVKHKYKEGMFDQVDRVLYDLKNNRASRRIITNIYVHADLHEMNLYPCAYS
                     MTFNVSGDVLNGILNQRSNDVLVANNWNVVQYAVLMHMMAQVSGLKAGELVHVIADAH
                     IYDRHIPMVEELLTRESYPAPTFWINPEITDFYDFTVEDFKLKNYQKNEQIKDIEVAV
                     "
     gene            888..1388
                     /locus_tag="PLF14_05430"
     CDS             888..1388
                     /locus_tag="PLF14_05430"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004223430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOT70770.1"
                     /translation="MNIIVAVDEHFGIGNLGKIQWKIKEDMDFFRRSTLNKTVIMGRK
                     TLDSFKCGKPLKNRHNIVLTRDKCFKRENVTVVHSVKQALEKISDIDTGDVYIIGGQS
                     IYEAFLPYCDKAFVTKVHGVFDADTYFPDLDKSDEWKLIHQSDRQITQSGIHITFCEY
                     ERVRHE"
     gene            1381..2637
                     /locus_tag="PLF14_05435"
     CDS             1381..2637
                     /locus_tag="PLF14_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70771.1"
                     /translation="MSDGGKKSIRINKTYLILGVIYLIAFVVRIFIAGSITGHLTDMG
                     CFSYWGEQAYNEGLSEFYNGKLFCDYPPLYIYVLWVIEALRRAFGIVFAMPAHLILLK
                     LPTIIFDMLSAYFVFKIAKTRFSDKTSIILSACMAFNIMYILNSSAWGQIDSILTLLI
                     AITAYNIMNNKLHFATVAYIFAVLLKPQAFIFAPILIFAYIKSRNLKKVITSLLIGLG
                     VFVVVILPFAIGQKPLWIFGLYFGTMGQYAYATVNAYNLYALLGLNWIPDSEIMLLFP
                     YKIYGYIAIVGVCALTTAAFIKGHKKPGFTFATAGLLMTVVFTFATKMHERYIFPSIL
                     LVLAAFIFSNDRRFLYIHILQTLTCFANCAYILHLNYKNSYDFFSPFVAIVSAVTVFT
                     TLWYIKVYIQLYIMNKDNMPCLKSEQ"
     gene            2672..3847
                     /locus_tag="PLF14_05440"
     CDS             2672..3847
                     /locus_tag="PLF14_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70772.1"
                     /translation="MFKKLSTLLLIVLVIGCLASCAKFSHLESGSDNTDTRPSHSITH
                     SSDDTKPEEYYTAFRQGEFSVSAYLVTPYGMYINIGKYVYYSEKGNTEYVKLCNKPNC
                     NHSTEDCNAYTGAHQIGYYKDKIYYTSFKMNVNTLNCMNMDGTNHKEIKVLNNAYIST
                     FGYYHNGYFYYMLGFPGLQLIGVTNDDNNLYRVKVDDNSKPEIILTGDIIKKSMFYVV
                     EDTIYLIVREDGGFGCCLYSYSCKTGALTKISDCWAGISYYTKDYGYCYRINEGIYKY
                     NVETGEVTLDKAIKFNNHGHCEVRFYPDYIYLIHNRNDDYRALREQDLVLYIYNWDYE
                     IIETVLLDFINKGKRGNFITDVGDYIIFASDMDNKPDYYIDKSEIGTDKFAFHKIEN"
     gene            3991..5202
                     /locus_tag="PLF14_05445"
     CDS             3991..5202
                     /locus_tag="PLF14_05445"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HOT70773.1"
                     /translation="MKHKRQIINSIKKAIVVVLILILSVLFVSCSDYLEAVGNIAESP
                     VLPSKTPKPTETPTEKPYETPEPTQTPVESIVPTETPAPTPDPSLILTNNPLTGKEQY
                     MHEFSLTAKPVAVILENKRQSLPQIGISEADIVIEMLVEYDISRFMAIYQDPYKVERI
                     GAVRSLRSYFTKIALAFDSYIFHYGFSDYGEENAKQDLIDRGLTTVNGIYGEDNGHPY
                     WRDQDRIASGIESLHSVVTAGENVLASMKSLSSDKISKENRDNVFNFSAESSTKNGKE
                     ASKVYLTGAYISSPYFIYDQAKGEYTRYQFSDVQIDGNTNQPVTVENLFVLKMETRYT
                     EDAAKHVLITTHGTGTGYYINDGKYIEIQWSRETDWSKFVFTANGEEIKVAPGNTWIS
                     CIPSDKYISIK"
     gene            5210..6136
                     /locus_tag="PLF14_05450"
     CDS             5210..6136
                     /locus_tag="PLF14_05450"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOT70774.1"
                     /translation="MRKIIALFFILLTVLNLSCCVKPTEQPSFTHAPTVMPEVSSTEI
                     IQPTESIPTKEPATPSPTPTVIPPGSTYIEFYDLYADLTKTGEMKKDTIHWSEKGQRT
                     EVFDKHNVITTIDTEEKVMYLTFNLGYEHVRGNTEKLLEILRNRNISATFFLVGEFIE
                     VFPEKVRAIYADGHTLGAHSYYHIPPSHTMTDEEYIEDFIKTEKLLKIALDSDYKRMR
                     YYRPISGEYCERDLYIANALGYTTVLFDATYSDWNRTVINGYEYAYNELVKDIHKGAI
                     YQLHIVTPDNIEALPVFLDELIGDGWMFKAFN"
     gene            6196..6474
                     /locus_tag="PLF14_05455"
     CDS             6196..6474
                     /locus_tag="PLF14_05455"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOT70775.1"
                     /translation="MKSRTKRRLFTLISFAFIVYFIYIIFSQQPRIDMLMTKKQKVEE
                     KIKEQDAIHEQLIYYQSIAGTDRYIEMMARIRLGYVKADDLIFIDADR"
     gene            6494..6763
                     /locus_tag="PLF14_05460"
     CDS             6494..6763
                     /locus_tag="PLF14_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70776.1"
                     /translation="MWTIRFCDSNTRYIVLVREALFDGAVLYSKTEETILRLCRKLTV
                     VTQERLGVRCEPYKYMSDPKKERFYEVKPTTIDIVYESLLSILNH"
     gene            6810..8075
                     /locus_tag="PLF14_05465"
     CDS             6810..8075
                     /locus_tag="PLF14_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70777.1"
                     /translation="MKKPSKGFLILALVFVLLLTACTKQELPKETVSSEVTTPPAENP
                     KIETEEPANTYDIEKYDDMVVELFALKEDNNGNYCDMFLRINYADKIIEIPDRKQFEL
                     SKPLIGILDINGDGNGELFLKFLEKNEQNELVENVYFMDINTEKELSLSDLGLEDTAE
                     VTHGTAEFIVNDKTVTYDFNELHFKGKENLNKILKKKDIQKAPMLYTDNLTLMILSEP
                     MPSTGRNQRVTILKYDDKFAFYRYQNMPMPFPKYNNGKEEITYESSVQMGLIDLDNDG
                     IKEIVITMWVGKGTGYSATDLHIIDSTTMEEIVTLTNDDIAILISSYIDAKIVTKEEQ
                     VIINICVNNDSDNTYTHTVKRNENITYFETPSFTQSVGFAIDETNNKVMIDAGMMIAA
                     TWYAGSFSADITVVDGKVHFANINNIFFE"
     gene            8123..9838
                     /locus_tag="PLF14_05470"
     CDS             8123..9838
                     /locus_tag="PLF14_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70778.1"
                     /translation="MKRLSNKNILLFAMIVMLVLLLTACTKKELPKETVSSEVTTPPA
                     EVVEDFVLIAELSPTDKYLSPILCGVKVYGKNKDNYGNYTEIKFVFKDKEWISDELKI
                     FPRMELRREDEHRMYLPTLENRDFISVIYGEKDSEDTIRENIIFFDWQTFMQIKPEEL
                     NILIVHKQSTDVVVSDDKSYTVTVAEKSKNDRDIFDEKKNIMKYNYGEVNVFALRKDK
                     MGNYYDLYARIKNTGKNIKIPDRKQYEYGGPRASLLDLNGDGNDELVLVFWERNPEGV
                     LTQSVCFMDIKAEKEVSLSDLGLEDIVEVTYQKEKRTIAHEDRVYKEYDVVACHHKRK
                     EDYNKDNLKTIPTEEIKRISGDIDLALSLLTDPILFITEKKRPDIFVLQYGEKIELYH
                     DWSMPMPFPKYNNGKEEITYENSVQMGLIDLDNDGIKEIVITMWVGKGTGYSATDLHI
                     IDSTTMEEIVTLTNDDIAILISSYIDAKIVTKEEQVIINICVNNDSDNTYTHTVKRNE
                     NITYFETPSFTQSVGFTIDETNNKVTVGAGMMIAAPWYAGSFNADITVVDGKVQLTNI
                     HFSFD"
     gene            9899..10171
                     /locus_tag="PLF14_05475"
     CDS             9899..10171
                     /locus_tag="PLF14_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002665927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HOT70779.1"
                     /translation="MSTTSLNIRVDKTVKKQADEVFNELGINMTTAVNMFLRAVIREN
                     GIPFALKLNTPDSITKAAIEEGKRIAYDDSVKGYSDLVDFKKVLEE"
     gene            10279..11985
                     /gene="argS"
                     /locus_tag="PLF14_05480"
     CDS             10279..11985
                     /gene="argS"
                     /locus_tag="PLF14_05480"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOT70780.1"
                     /translation="MKDIKTKIAKKLAGSIEQLSWTEIMDNLEYPPEPHMGDLALPCF
                     KLSRVMRKSPMAIADSLVDSLDIDVVERCESKSGYLNIFINREYLVNNVLKTIVEKKE
                     ETGKSNEYAGKTVCLDYSAPNIAKPFHIGHLRSTVIGHAIRRLHEFRGYKCVGINHLG
                     DWGTQFGKLIVAYKKWSSKKAVEEKGTEELVRIYVKFHEEAEKDPSLNDLARQWFARL
                     ENKDEDAMELWKWFKKISLIDIEKTYKVLDITFDSYAGESFYYDKTQPVVERLKERNL
                     LIESDGAMIVNLEEYNMPPCLIVKSDGASLYASRDIAAAIYRKETYDFDKCIYVTATA
                     QDLHFRQWFKVVELMGYEWAKNLIHVGFGMVSMGGAKLATREGNIVYLDDLFSEASKR
                     VLDIINEKNPDMEDKEQKAQDIGVGAVVFSDLSNNRIKDIDFSWEEALNFDGSTGPYV
                     QYTYARACSVLRKSELSEKDINMKDIKPEHMTNDDEFTLLKTLSLFNEKIDQALRDLE
                     PSCISRYLIDVCMDFNKFYNAHSILGAEDNKKFRILLTAAVKYVLANGLFIIGLKRTE
                     RV"
     gene            complement(11982..14297)
                     /locus_tag="PLF14_05485"
     CDS             complement(11982..14297)
                     /locus_tag="PLF14_05485"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOT70781.1"
                     /translation="MYDISVKKNIEWKAFWLHDPIFKKQPVIQPVDAKRSKYLRTEVQ
                     NRHTLFRKEFEIKNIPIKSAKLYITADDCYKAYINGKFLAYGPAQALAFAYNYNAFEI
                     KKLLKPGLNTLSFHVFYQGMLNMALISADNLNGMICMLEIEYKDGNVQRISSDHTFKC
                     YPLMAYSSDKVYGYETQFAENIDLNLFPYGWNQTGYQDCDWITPFISAIPYPMSYTLV
                     PQITPTVSFSISYPVEIIKKKKGNYLLDFGQEICGNTVITAKGKKGHVITIWHAEELT
                     EDNKSARYNMRCNCDYEETITLSGKEKDKAEFFDFKGFRYIEVFNYPGKLTKKSIYVH
                     HRHYPIPKNHSKTQSDNSLFQRIWEMCEKGIIEGTQESYLDCPTREKGAFLGDGLVTG
                     LAHLAYTNDSRIMKKFLYDCMHSARLCPGLFCTTVTYDASQIIDYSLVFPVMLNKYFE
                     YTEDISTLKDSLNVLEGIIAYYAQFYRPEGILGHIKHVHREQYTVLVDWPAVYRDGYS
                     DEITKGPNCFGNLAYIGILKESAKVYSYTGNELRAEELSKKAKQLEKAVINAFYDNET
                     GLFNITKTSDIHNFHDAVFALYFDLELPKGKKPLLDYIKRRGIVCGVYFTYYYFSVLY
                     NHKEYDKIFDLIVSDEKNSWKTMLNTGATTCIEAWHPDDKKNCSFCHPWSSTPMIFND
                     IFFGISSYGTGYKVLQFKPRIPKQFNKGTYTKPTPLSDITVSFERKDKKIIYNVTLKK
                     ACRMYVCLSDKEEGYFADASVGEHKFIQITD"
     gene            14379..15002
                     /locus_tag="PLF14_05490"
     CDS             14379..15002
                     /locus_tag="PLF14_05490"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOT70782.1"
                     /translation="MLIHYLLLISASVMLSKLSVRPLRALSYTDTPLFYDDMGCMLSV
                     LLSVSFTVSFYVKTDIISKILMVILLFCLCCITYTDSQAGYIYDRFHIVILLIGIAQL
                     FIKGFSITDIQDSISGCIIGGGFLQLMNCLSILVFKKEGIGGGDVKLCFACGLILGLK
                     GMISAFIFAFFPAALYAFISGAVRGREVALGPFLCVSIAAVYCIGML"
     gene            15029..15613
                     /locus_tag="PLF14_05495"
     CDS             15029..15613
                     /locus_tag="PLF14_05495"
                     /inference="COORDINATES: protein motif:HMM:NF014417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HOT70783.1"
                     /translation="MTLGYENVISSRQGYINSWYVFHVKSGEEIKARDNLIRICERKG
                     IKLDFVIPTAIKFIKKKRQRIKSYKTVFPGYVFANGVIDVEIYNTLKGIPGVYRLLRD
                     RSTFALLPVPAEEMELLAGIMNVHGVIEAPEVSFREGQSAVITKGPLTSFKGKIVAVD
                     KHKNKLTLLVLFLGEERRVEIGFLFAESLDKENS"
     gene            15613..16191
                     /gene="yfbR"
                     /locus_tag="PLF14_05500"
     CDS             15613..16191
                     /gene="yfbR"
                     /locus_tag="PLF14_05500"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: protein motif:HMM:NF003009.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HOT70784.1"
                     /translation="MKKGSFFAFIHRIGYINRWGLMRNTSYENLKEHSYDVAVIAQGL
                     ALIGNAKFNKNYDVNRITAAAMFHDVNEALTGDLPTPVKYANKEMEKAYKKIERDAVN
                     NILKMQDDSIRESYEQYFNPSEEDRLVIKAADKISGFIKCKEEKDRGNKDFDKAYDSL
                     KKSIEETDLPEVKYFIENMLPHYNLVLDEFLE"
     gene            16345..16779
                     /gene="rplM"
                     /locus_tag="PLF14_05505"
     CDS             16345..16779
                     /gene="rplM"
                     /locus_tag="PLF14_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOT70785.1"
                     /translation="MKTYMAKADSIEKKWYVVDAEGRTLGRLASEIAKILMGKNKPEY
                     TPSVDTGDYVIVINADKVVVTGKKETQKLYRRHTGYVGGLREVVYRDLMAKHPDRIIT
                     YAVKGMLPKNKLGRHMLKKMKVYAGSEHNHQAQNPIVLDLNE"
     gene            16794..17180
                     /gene="rpsI"
                     /locus_tag="PLF14_05510"
     CDS             16794..17180
                     /gene="rpsI"
                     /locus_tag="PLF14_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOT70786.1"
                     /translation="MTQYYGTGRRKSSVARVRLVTGTGKITINDRDIDDYFGLETLSI
                     IVNQPLEATKLRGKYDVLVNVKGGGFTGQAGAIRQGIARALLEANPDLRAELKKAGYL
                     TRDPREKERKKYGLKKARKASQFSKR"
     gene            17375..18715
                     /locus_tag="PLF14_05515"
     CDS             17375..18715
                     /locus_tag="PLF14_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004800342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT70787.1"
                     /translation="MERFILKKLLDWKNSPYRKPLILKGVRQVGKTWVLKEFGKRYYE
                     NTAYFNFDENLEYKQFFETTKDVDRILQNLMIVSGQTIVPEKTLIIFDEVQDCPNVIN
                     SMKYFCENAPQYHVACAGSLLGIALAKPSSFPVGKVNFMQIDPMTFEEFLLANGDENL
                     LAFLRSCDSIEPIPDAFFNPLYEKLKMYYITGGMPESVLMWTEARDVSAMQEALSDII
                     GAYERDFAKHPDISEFPKISMVWKSVPSQLAKENKKFIYKVIKEGARAREYEDALQWL
                     VDARLVHKVYRSSAPGLPVAAYDDLSAFKIYLVDVGLLRRLAQLAPTAFGEGNRLFTE
                     FKGALTENFVLQTLVTQFEAMPRYWSQSNPPYEVDFLIQRENDIFPIEVKSETHTAGK
                     SIKKFKELFPDKVKLRIFFSLDNLKLDDDVLNIPLFMADRTDKLIGLALEQRKV"
     gene            18911..20269
                     /locus_tag="PLF14_05520"
     CDS             18911..20269
                     /locus_tag="PLF14_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005428036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT70788.1"
                     /translation="MLKRKIYKQIEDFYKCHSNKALMITGARQVGKSYIIEKYAKEHY
                     SSFIRIDFIENPEYIALFDNVTSATDVLLKLSAIFGDKMIPMKTIIFFDEVQECKELI
                     TQIKYLVSEGSYTYILSGSLLGTVMNDIRSIPVGYLQILTMYPLDFEEFAIANGVGEN
                     IFDMLESAFNSLTPIDDFIHKKLLSLFELYLIVGGMPAVVSSFISTKNLKFVLDTQKM
                     IIELYKRDISKYDKENKLYLNNIFDLIPSELNAKNKRFILKKLNENAKLSIYLNSFLW
                     LKNAGVAISVFNANEAKVPLVLSKSSNLFKLFLNDVGLLAALYGEGIQLKLLSGELNI
                     NHGAIYENFAATELTAHGFIPYYFNSKKIGEVDFLVESEGKVVPIEIKSGKDYYRHSA
                     IDNMLECKNYDIEKALIFCNGNIEKKDKVVYYPIYMLSLIRKKDISEDIIVETDFSAL
                     KE"
     gene            20648..21880
                     /locus_tag="PLF14_05525"
     CDS             20648..21880
                     /locus_tag="PLF14_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT70789.1"
                     /translation="MKLYRRENYLSKIRGFYHDVGMIKVITGVRRCGKSCLMQTIAEE
                     LQEQGIKPSNIIFIDLRKHAYKSIQTPQALEKTIDDKCNSDEKKYLFIDEIQNVKGFE
                     LVLEAYRSEGEYSIFITGSNSYLLSGELITALTGRYIEFEMFTLTFEEYLDMKEFYGK
                     PLSPNLVLELDNYIMEGGFPKALEYDNLKDKRTYVNSVISEILKKDIKQRVKIRNFEV
                     FQKIQTYIINNFGATTSISNILSELKKDGLNIKRETLARYIQLLIDAKILYECKRFDL
                     KSRRSIRGEQKYYLADLSFYFVTNVDNRINYGPVLENIVYQYAASKDYEISVGRIGSL
                     ECDFIVRDIEMNYAYIQVAMTIMDSREAEEREYAPLEMIRDNYPKYLLTRNDLLQRRN
                     GIKHRNIPQFIRDNDLFD"
     gene            22060..22311
                     /locus_tag="PLF14_05530"
     CDS             22060..22311
                     /locus_tag="PLF14_05530"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT70790.1"
                     /translation="MKGGTLRRFVILSGARRVGKTTIMYQMIDCLLGEGISPRNILYV
                     SFDNPIVKFVDIETVISGYESLYPIEGTKYVFFDEIRYA"
     gene            22757..23236
                     /gene="tsaE"
                     /locus_tag="PLF14_05535"
     CDS             22757..23236
                     /gene="tsaE"
                     /locus_tag="PLF14_05535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOT70791.1"
                     /translation="MEKTYVIATCNEEDTIRLGKILASVFEDRVIAVCGDLGAGKTHF
                     AKGIALGMGIAKHVTSPTFSILNIYRNGDKTFYHMDAYRLTDEESAYNAGIEDILQPK
                     GITLIEWAENVTDILSDGYVSVNIEKGSDENKRLLYVTADRQSVDRMALLIHEDTDR"
     gene            23217..23921
                     /gene="tsaB"
                     /locus_tag="PLF14_05540"
     CDS             23217..23921
                     /gene="tsaB"
                     /locus_tag="PLF14_05540"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOT70792.1"
                     /translation="MKILIGDTCLKTASAIIVNGKKILAYMQSFGEEPHSVMFPKLVD
                     QVLNIAETDFNSLDAVACVYGPGSFTGLRIGLTYLKTLAYTLDIPFYVLNTLDVLANM
                     EEGKRYVVSIPLIEARNNEVFTAMYESEKGIYKRITPYAAKHIDEVVEDAVSLCAKDD
                     KALILLSGDAQKKHIAEFQEHLTDKVLPYSEERDNVDLSRAHLLISEQCRTDPFTAQP
                     FYLRESGAVRMKKKKQ"
     gene            23918..24361
                     /gene="rimI"
                     /locus_tag="PLF14_05545"
     CDS             23918..24361
                     /gene="rimI"
                     /locus_tag="PLF14_05545"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOT70793.1"
                     /translation="MNIRIKRADINDVDRIFAIEKETFNDYWSYESLYEDICRTPMSF
                     YLAAVFDDEVVGYIGMWHVLDESHIMNIAVDKRYRNKGIGTLLLSALIDYSGQVGIKR
                     MTLEVREHNDAALRLYEKHGFVKDGIRKEYYRDTNEDAVIMWKEL"
     gene            24362..26143
                     /gene="uvrC"
                     /locus_tag="PLF14_05550"
     CDS             24362..26143
                     /gene="uvrC"
                     /locus_tag="PLF14_05550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOT70794.1"
                     /translation="MTVLNEKIAYLLSTCPEKPGVYLMKDNKGNTIYVGKAVNLKNRV
                     SSYFTNMSAHSYKTKKMVSNIETFEYIVLDNERQAFLTESDLIKSLRPKYNILMKDDK
                     SYPYIKITTQEQFPGIYVTRNRSDKNAKYFGPYPGRVSKKNTVTLLQSAFMLRSCKKM
                     AKRPCINYDMHLCLAPCSEDCDKNKYREAVDAALRFLSGNGRDLIDEYKSKMQAESDN
                     LNFEKAAYYRDRINLLTTISDNYILDTDRNSADIIAYARMKDFVSFFIMNIRDGKVAD
                     KKTVNIHQERIEDTDIGYEFIRQYYDTNIPKEIVVYNEIMNREDTEQWLFNNCGRKVS
                     ITVPKKGKLKKLADAAYENAVVTLNLFMGQYKNSAGTSMVYSLASFLGLETVPDVIEA
                     FDISHLSGQDAVASQAVFKEGVPYKSGYRRYIIKGDNTRSDTDSLREVMERRMKRGDY
                     PDLFLIDGGFNQVAAVCDIVGDRVPVAGMVKDDKHKTRALIYDAKEYDLQSDRNLFVF
                     ISSIQNEAHRFAIEYNRKKRAKKVYESELDNIPGVGENIKAALLKHFGSVRAIKAAGE
                     QQIAKVQGVGVKRAKAIYDYFHKPYGG"
     gene            26144..26791
                     /locus_tag="PLF14_05555"
     CDS             26144..26791
                     /locus_tag="PLF14_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT70795.1"
                     /translation="MAVYAIGDLHLSFQTDKPMDIFGPGWIGYEETLYENWQNTVKPQ
                     DSVIIPGDFSWAMYLNEAVEDFRYLNGLNGRKILLKGNHDYWWETKTKMNRFLKENGF
                     NNIDFLHNNSFEIEGVNFCGTKGYDSKEADEKIINREIIRFSISYESIRNKSARTIAV
                     FHYPPEKELLYMMAEYNIEICVFGHLHGLEKSSYNKPGFTLVAADYVDFKPVLLI"
     gene            27065..28018
                     /locus_tag="PLF14_05560"
     CDS             27065..28018
                     /locus_tag="PLF14_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOT70796.1"
                     /translation="MNISQVKKVAEEIRENIGKVIIGKNDIIDLVLVSLLSNGHVLLE
                     DVPGLGKTMLAKSLAKSIDAHFARVQFTPDLLPSDLTGVSIYNQKQGEFIFKKGPLFT
                     NIVLADEINRATPRTQSALLECMEEHQITADGVTYRLDEPFFVIATQNPLETQGTFPL
                     PEAQMDRFFMRLSIGYPQIEEEIAILTRFERDMPLEELKSVATKDVILKASQAVKTVA
                     VSDDIKDYIVRIVTATRNSVRLILGLSPRGSLALMRAARAYAAIKGRDFVIPSDVKTV
                     APHVMSHRVMLKGHSLSYGMKGTLDVINEILDTVPVPTEEV"
     gene            28025..29179
                     /locus_tag="PLF14_05565"
     CDS             28025..29179
                     /locus_tag="PLF14_05565"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOT70797.1"
                     /translation="MELILIVTSLVIFFVAEIVFFRFFVLRKIEYSLSFSQSEAYEGD
                     DIFIDEVINNNKSVAVPWLKADIHASKWLQFANTSSTIIDEERCVSSGFSLKGHQKVT
                     RRWYTRCAKRGVFTIVNTTLTAGDLLGLMQLSDAYPANTRLMVFPGVLRLEEAFFSNR
                     DLIGEMIVKRFIMDDPFIVKGTREYVQGDPLNRINWKTTARMQKLMVKENDFTAKKGI
                     TVLFNIKSYAMEFIEVKYKEVIELGIKVVNTVFEKGYGMGFPLRLGTNAGILSNPDEM
                     IFTDSLFGPEHMRHLKVILAELILKNHLDFPDFLERHYDDIYDTQVYIVSAYLNDEIV
                     EKADILRRNGNTVKFLILNYTSDATSNRGMEVFLLDRNTEFLKEGEYHGT"
     gene            29169..30581
                     /locus_tag="PLF14_05570"
     CDS             29169..30581
                     /locus_tag="PLF14_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70798.1"
                     /translation="MAPEKQIRRKILPLLGNIGSVGGLTFAIYYEIFPETVYHWLGFC
                     FLMMVIGTVAGVMIVDFYDNKGEGIVVADVDRVRLFLQPAYIYAVIPFVLLFVLGLIV
                     SRGFVEALIGFALMPTIYFILGIGNGLFDYTQTANRKILYTALIPLIIAIFKAFVSSP
                     YGFYKDPVWIFGSLYMLFFMLTINRIKIEELFVLSKKVNVENKKSIRRRNDFLIMIFF
                     ALYLIIFVVRNTFTRISEWIFRNTVAIISFIINFFYKMRKVIEVEQVQEVVEEGIPDE
                     VPPVPPTMNPTAKTILIVIASLTIAFVLYFLVKKIIQLIRWLIGKASDTLTGGKRIKR
                     DTENEEFEEIVEFTKDKARRKRQKVIKYKYSLRELRNLDTYKEKVRYLYGFALERLKY
                     KNVKISKADTPEEIVDKLGTYENGNKLVDNVFKDFTDEYINVRYGNKETGQDENFMEK
                     ADAVDKGIEGIKVKEEKATA"
     gene            30686..33076
                     /gene="lon"
                     /locus_tag="PLF14_05575"
     CDS             30686..33076
                     /gene="lon"
                     /locus_tag="PLF14_05575"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOT70799.1"
                     /translation="MTEKKNNIEEIIENEDIKENPMKVEFTKIPLIPLRAVAVIPGMV
                     THFDVGREKSILALEKAMEENGQVFLVMQRDASIENPKRENMYEYGTIANVKQVLRLP
                     GRTCRILVEGIKRAKLEETVTVKGYYTAIVTIPKITEAIISANNILEAMHRKLTTLYE
                     EYVTLLGRISHEGYFNAVAADDIGVVADAMVGSMALKSEVIQSFIEELDGITRAEKLL
                     PLLVNEIEILKLERDLATKVKSTIDEKQKEYYLREQLKAIQEELGEYSEEVDEDEMGE
                     YIKAIESKRYPEEVRAKLKKEMSRLSKMSPMSPEASVIRSYMETLIELPFGIETKEKL
                     DIKHVGRILERDHYGMNKVKERIIEYIAAKKLSNSFNSPILCLVGPPGTGKTSIVKSI
                     AEAINRKYVRMSLGGIKDEAEIRGHRRTYIGSMPGRVVKAIKQAGSMNPLILFDEIDK
                     LGNDFRGDPASAMLEVLDAEQNNAFRDHYLEVSLDLSHVMFITTANTVETIPRALLDR
                     MEVIEVTGYTEEEKMHIANKYLIPKQLKKHGMTRKHLNITDSVLKKVISNYTRESGVR
                     NLEREIASLCRKVATRMQLEETDRVKLTVNNIEDFLGPQKYHYDTTFDKDEVGIARGL
                     AWTQVGGDTLTIEVNIMNGTGHVELTGRLGDVMKESAKAAIGYIRSQTDRLNINPDFY
                     KDKDIHVHVPEGAVPKDGPSAGITLATAIISALTNRKVRRDVAMTGEITLRGRVLPIG
                     GLKEKLSAARRANVRQVLIPMENIRDLAEVPKQITDELEITPVSNMDDVLKNALAE"
     gene            complement(33112..34470)
                     /locus_tag="PLF14_05580"
     CDS             complement(33112..34470)
                     /locus_tag="PLF14_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015514945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOT70800.1"
                     /translation="MGFENRLTEGNVAKQLIKFSLPLLLSNIVQALYSVADMFIVGWL
                     SGTYTISGVNIGAQVTMIVTNFVIGLAIGGTVLIAQYIGAKKHEEVNKTIGTLFSVMG
                     LLAIFFSIFMTFFVDVMLKVLNTPEESYSMAKDYVLICMWGIIFVFGYNAISAILRGM
                     GDSKRPLMFVGIACVINVVLDILFVGPLGMKAAGAALATIISQAISMILAVIYLKRSG
                     FIFEFRRENFKIHKDKLKLLLKVGIPSSAQSVVVGISFLVLTSMINDIGGVTASAAVG
                     IVGKFNSFAILPAAAMSASVSAMAAQNIGAGLYDRASKTLWTAIAISFSISIVIFTFV
                     QVFPESILLAFKAEPDVVSIGVAYIKAFSLDYLIVPFAFCFNGLLNGAGYSIISMTSA
                     MVSSLVFRIPLVLLFAKVFETGVGGLGYAVPISTCFSMIIGGYFYFSGKWKQDRLKIQ
                     KP"
     gene            34646..35794
                     /locus_tag="PLF14_05585"
     CDS             34646..35794
                     /locus_tag="PLF14_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HOT70801.1"
                     /translation="MRTIAEINEKIKKGKAVVVTAEEVIDIVQEKSYKKAYETIDVVT
                     TGTFGPMCSSGVFINFGHSNPPIRMQSILLNDVCAYGGLAAVDTYIGATEVSQKRGME
                     YGGGHVIEDLIAGKDIKLHALSSGTDCYPRKEILTTINKDNINQAYMFNPRNAYQNYP
                     AATNSTNKTIYTYMGTLLPNYGNITYSTSGELSPLLNDPYLRTIGVGTRIFLGGGVGY
                     VAWQGTQHKVDVERSANGIPLVHAATLAVIGDIKGMSTEYVRGAVFDKYGVTLFVGVG
                     VPIPVLDEEMLKYVSVKNSEIYTKLVDKSGNVNIDKKYNYAELRSGTVKFNGKAVHTA
                     PLSSLDVARKIACTLKEWINRGTFELTEKVADLPCESVVHPLEIREIK"
     gene            35794..36198
                     /locus_tag="PLF14_05590"
     CDS             35794..36198
                     /locus_tag="PLF14_05590"
                     /inference="COORDINATES: protein motif:HMM:NF020940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOT70802.1"
                     /translation="MSKLKLKLSFDKDCVTKPVIYELVKKYDVMTNILSANIEYGNRG
                     NMILEIKGKEENLSLTLEYFKSLNIKYEIYAEAILWDESKCINCGSCTAVCPTQALTM
                     GKDDLLHFDKDKCVVCQLCINACPIRIISVEL"
     gene            36206..36922
                     /locus_tag="PLF14_05595"
     CDS             36206..36922
                     /locus_tag="PLF14_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HOT70803.1"
                     /translation="MYQPRDYRKSFTDRLKWYNVCVEETDLMIGTDEDCSELVLKTVR
                     TLRKEIYAAIRDNSEFLTSLVPVEYKGEGVVEAMVEAAKLAEVGPFAAVAGAINDMLA
                     EKLMDKSKQIIIENGGDIYIKSDQDRLIGIYAGNSVLSGKFAIEIQKDLFPVGVCTSS
                     ATVGHSFSMGKADAAVVISKTCAVADAFATAFCNRIKKTEDIAPALEWVNGFDEVIGA
                     VAIINDKIGAIGNIKLVKWN"
     gene            36924..37727
                     /gene="proB"
                     /locus_tag="PLF14_05600"
     CDS             36924..37727
                     /gene="proB"
                     /locus_tag="PLF14_05600"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOT70804.1"
                     /translation="MRTTLQQADKIVIKVGTNTLTHANGKMNLRSMELLVREIAELVN
                     AGRKVILVTSGAVGVGLGKLNLMKKPRNLAARQVLASVGQCELMHIYSKLFSEYSITV
                     GQILLTRDVTENPVKAENAYNTFKGLMDMGIVPIVNENDAISTDQLAIVDSFGDNDTL
                     SAMVAKLIKADLLIILSNIDGLYDKDPNINEDAKIVDTVTEINKEIESYASDLTSSLG
                     RGGMKTKLTAAKTCLENDITMAIINGDNMKNISALLDGDNIGTLFTNKR"
     gene            37741..38985
                     /locus_tag="PLF14_05605"
     CDS             37741..38985
                     /locus_tag="PLF14_05605"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOT70805.1"
                     /translation="MAYIDELGIKALKAKEQLIQIDTEKKNAALRLMAKNLLKHKEAI
                     KKANTDDVINAKEKGTKEALTDRLILNDARIESMAKGLMEISQFPEPIGNVLYERVLP
                     KGMKLKKVSVPMGVIGIIYESRPNVTADAAALCFKAGSAVILRGGSEAINSNKAIVAA
                     LKDALEEIGINKDSIQLVEDTGRDTATQMMKADNYIDLLIPRGGAGLIASVIQNATVP
                     VIETGTGNCHLYIDSIFNEKFALDITVNAKCSRPGVCNAIETLLVHKDVYKDILPKIE
                     EELSKYKVQLRACEQSVKVLKNAIPATEADWSEEFLDYILAVKVVNDIEEAIEHINKY
                     GTKHSEAIVSDIEENRNKFTKNVDAACVYTNTSTRFTDGGEFGLGAEMGISTQKLHAR
                     GPMGANEITTYKYIVEGDGQIR"
     gene            complement(38986..39924)
                     /locus_tag="PLF14_05610"
     CDS             complement(38986..39924)
                     /locus_tag="PLF14_05610"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT70806.1"
                     /translation="MRKKPLSEVTMEFRTLKEDEFKEWTAFCGCIFPVGEAYFIRHFM
                     NDPYRDINGIFIAKDPTGIVSSVRVFTRYLRIKDNVIKVGGIGEVCTKPSRRGQGLST
                     KLLTMAIDYMEKQDMPISRLTTGNTHHYEKLGWFSLRFCHKVYRLRKSVLPEHYSVVQ
                     SSPVDIDAVKSLFEEHAHKLCPVYVRNDEYYDKWVRSELINTYFLIKDRKVVSYADCV
                     FKKGQGVEVREYIGGDEICMMKPFLYAICKEEGWPKICGVSAPFAKDKESFRLYNSGQ
                     MFRINVPFEIDGKIISCEQDLLPYLSELPYLPIDGY"
     gene            complement(40035..>42289)
                     /locus_tag="PLF14_05615"
     CDS             complement(40035..>42289)
                     /locus_tag="PLF14_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70807.1"
                     /translation="PTATPTPTPTATPAPTATPTPTPTPTPTPTATPTPTPTPTATPT
                     PTPSAAPFGIGNAYASGPKEVTVEFSAPAQAGAQVVLKTGGINVTGTSLNWASDRRSV
                     RITRTAAYAAGEYTVTVSDSSKTFTVQAEKLTAISITAQYVFPMDNQDLKVVFTNQYG
                     GEMPLTAYTNFTLSAVCQAKPSVTLNLNKTAAGFILKGAQGLAANDAVNIVIAENTTG
                     TAVTKSVTLSESPKITTFAFGQVQLASGTTVILVNSSGHSLTVDAKDQYNQAYTIVPS
                     DIGLNKPVILTSNDSAIIDASKITVANGKLNFPIGAIGAKEGTVTLTAVGQNISSSIQ
                     ITVKTPAAVKTLTITGVEGTVFVNQWVPLVVTAKDQYGNPTTLDTLDPTKLMMFSTNT
                     TVVPNTNIQFNGATKKIEIKGNSSGSATLHISYDGITQGTLDVQVLEAPVPTYITDVT
                     MPIYYQKNSNIIMAADMLKIADQYEKEIALTPAHSVIVTSSNISGAKFTEGTMTGAGY
                     TMSAPATSGSYTLTFTLKKGASTVNTYDMTVLVVDDDKIVSYKIEDIPTMYGGATDTT
                     TDTGHWKPIKISGLLANGKSVNLAYTGTLPSGIDQITCTNDSGANNFVIEDVSGVKTL
                     KAQNVAQTMTTTIKLWKAGQEIANLNVTASGAAPTLTHLAATEPTVTKALSGGVFNIT
                     AYVDAKDQYGVPMAFAAIAGSKTFMSTKTSVAVVNSTGIVTPVATGTCQIKIYFASAD
                     KTLAINLTVN"
BASE COUNT        13750 a         7353 c         9454 g        11732 t
ORIGIN      
        1 agcgaaatat acaagaagga ggagtatgaa cagtcactga ctgtatcata caagttcaat
       61 atgagcagag ccgatgatat attcaaaaat atgtgcagag atataattga taacggattt
      121 tgggataccg gtctggatgt gcgtcccaga tggttagacg gaacgcctgc tcattctgta
      181 aagaaattct gtgtcgttaa cagatatgat ttaagtaaag agtttcccat attgacactg
      241 cgtcccacaa ggtttaaggg agctattgat gaaatgctgt ggatatggca gaagaagtcc
      301 aataatataa aagatttgaa cagtaaaata tgggatgcct gggcggacag cgaaggctcg
      361 ataggtaagg catacggata ccagttgtcg gttaagcata aatacaaaga aggaatgttt
      421 gaccaggtgg acagggtttt gtatgatttg aagaataaca gagcaagcag aaggataatt
      481 accaatatat atgttcacgc cgaccttcat gaaatgaatc tgtatccctg tgcatacagt
      541 atgactttca atgtgtcggg tgatgtgctt aacggtatac tgaatcagcg ttccaatgat
      601 gtgctggttg caaacaactg gaatgtcgta cagtacgccg tacttatgca tatgatggct
      661 caggtttcgg gcttaaaggc gggagaactg gttcatgtaa tagccgatgc gcatatttat
      721 gacagacata ttcccatggt ggaggagttg ttaacaagag agtcgtaccc cgcaccgaca
      781 ttttggataa acccggagat aacggatttc tatgatttta ccgtggagga ctttaaactg
      841 aaaaactatc agaagaatga gcagataaaa gatattgagg ttgccgtatg aatataattg
      901 ttgcggtgga tgagcacttc ggaataggaa acttaggcaa aatacagtgg aagataaaag
      961 aagatatgga ttttttcagg aggagcacct tgaacaaaac cgtaatcatg ggcaggaaaa
     1021 cactggattc tttcaagtgc ggaaaacctc tgaagaacag gcataacata gtacttacaa
     1081 gagataagtg ctttaagaga gagaatgtaa cggttgtgca cagcgtaaag caagctttag
     1141 agaaaatatc cgatatagat accggtgatg tatacataat aggcggacag agcatatatg
     1201 aggctttttt gccctattgc gataaagcat ttgtaacaaa agttcacggt gtattcgatg
     1261 ccgatacata ttttcccgat ctggataaat cggatgaatg gaagttgatt catcagtcgg
     1321 acagacagat tacccaatcg ggtatacata taactttttg cgagtatgaa agagtaaggc
     1381 atgagtgacg gcggcaaaaa atccatacgg ataaacaaaa cctatctcat attaggtgtc
     1441 atatacctca tagcatttgt tgtccgtata tttatagcag gttccataac cgggcacctt
     1501 accgatatgg gttgtttcag ttactggggc gaacaggcat acaacgaagg gctgtcggag
     1561 ttttataacg gcaaactttt ctgtgactat ccccctcttt acatttatgt actgtgggta
     1621 atagaagcat taagaagagc gttcggtata gtatttgcaa tgccggcaca tctgatattg
     1681 ctcaaacttc ccaccataat atttgatatg ttatccgcat attttgtatt taagatagct
     1741 aagacaagat tttccgataa aacatcaata attctgtcgg cctgcatggc attcaatatt
     1801 atgtatattc tcaactcttc ggcttggggg cagattgaca gcatactgac attgcttatc
     1861 gcaattactg catacaatat catgaataac aaactgcatt ttgcaacggt ggcatatata
     1921 tttgcggtgt tactgaaacc tcaggcattc atatttgctc ccatactgat tttcgcatat
     1981 ataaaaagca gaaatcttaa gaaagttata acttctcttt tgataggact gggcgtattt
     2041 gttgttgtaa tattgccctt tgcgataggt cagaagcctt tatggatatt cggattgtat
     2101 ttcggtacca tggggcagta tgcgtatgca acggttaatg catataacct gtatgctttg
     2161 ttgggactca actggatacc ggacagtgag ataatgttgc tcttcccata caaaatttac
     2221 ggctatatcg caatagtggg agtgtgcgca ttgacaacgg cggctttcat aaaagggcat
     2281 aaaaaaccgg gatttacatt tgccacagcg ggactgctta tgacggtggt gtttacattt
     2341 gcaaccaaga tgcatgagcg atatattttc ccttccatcc ttctggtact tgccgccttt
     2401 atattctcca atgacagaag atttctgtat atacatattt tgcagacact gacctgcttt
     2461 gccaactgtg catatatact gcacctcaac tacaaaaaca gttatgactt tttctcgccg
     2521 tttgtggcta tagtatcagc cgtaacggta tttactacat tatggtacat taaagtgtat
     2581 atacagttat atattatgaa taaagataat atgccttgct tgaaatcaga gcaatagttt
     2641 tacaaactgc aatggaggtt agccggtgcg tatgtttaaa aaattaagca cattactgct
     2701 gatagttctt gtcataggat gcctcgcatc ctgtgctaag ttctcgcact tggaaagcgg
     2761 ttctgataac actgataccc gtccttcaca ctctataact catagctctg atgacacaaa
     2821 gcctgaagag tattacacag ctttcagaca gggagagttc tctgtttcag catatcttgt
     2881 aactccctac ggtatgtaca taaatatcgg aaagtatgtc tattactcgg aaaaaggaaa
     2941 tactgaatat gtaaaactgt gcaacaaacc aaactgtaat catagcacgg aagactgtaa
     3001 tgcatataca ggcgcacatc aaataggtta ttacaaagac aagatatatt atacttcttt
     3061 taaaatgaat gttaatacac tgaactgtat gaatatggac ggaacaaatc ataaagaaat
     3121 taaagtcctg aacaacgctt acattagtac tttcggttac taccataacg gatactttta
     3181 ctatatgctc ggttttcccg gactgcaact aatcggcgtt acaaacgatg acaacaacct
     3241 atacagggtt aaggttgatg ataattcaaa acccgagatt attcttacag gcgatattat
     3301 aaaaaagagc atgttctatg ttgtagagga tactatttat ttgatagtaa gagaagatgg
     3361 gggttttggt tgctgcttat actcatactc ctgtaaaaca ggtgcattga caaagataag
     3421 cgattgctgg gcgggtattt cctattatac caaggattac ggatactgct atcgcatcaa
     3481 tgaagggata tataagtaca atgttgaaac aggtgaagtc acattggata aagcaataaa
     3541 attcaataat cacggacatt gcgaagtgag attctatccg gattacatat atctcataca
     3601 taatcgtaat gatgattatc gagcactgcg tgagcaggac ttggttttgt atatatataa
     3661 ttgggattat gagattatag agaccgtttt gttggatttt attaataagg gcaaaagagg
     3721 caattttatt accgatgtcg gtgattacat tatatttgca tccgatatgg ataataaacc
     3781 ggattactat atagataaat ccgagatagg aaccgataag tttgcgtttc ataagataga
     3841 gaattagtta tggcaggtaa tgaaaggaac ttcatatgga actttatata gagaatcttt
     3901 tctctatgta tcaaagtatt tgacataacg gacatattga gtgaaaatat atgcgaaaga
     3961 taaatattgt ttggacatga ggacaaataa atgaaacaca aaagacaaat catcaattca
     4021 atcaaaaaag caatagttgt agtactgata ctgatattat cggtattgtt tgtttcttgc
     4081 agtgactatc tggaagcggt gggtaatatt gcagaatcac ccgtattgcc aagtaagaca
     4141 cccaaaccta ccgaaacacc gacggaaaag ccatatgaaa cgccggaacc tacgcaaaca
     4201 cctgtggaaa gtatagttcc gactgagaca cctgctccca cacccgatcc ttctttgata
     4261 cttactaata atcctcttac ggggaaagag caatatatgc atgagttttc actgaccgca
     4321 aaaccggtag ctgtcatttt ggagaataaa aggcagagct tgccgcagat aggtatatcc
     4381 gaagcagaca tagtgataga aatgctggta gagtatgaca tatcccgttt tatggccata
     4441 tatcaggatc cgtataaggt ggagagaata ggggcggtaa gaagtttacg ttcctatttt
     4501 accaagatag cattggcatt cgacagctat atattccatt acggattcag cgattacggt
     4561 gaggaaaatg ccaaacagga ccttatagac agaggattga ccacggtaaa cggtatttac
     4621 ggagaagata acggtcatcc ctattggaga gaccaagaca gaattgcatc gggaatagaa
     4681 tcgcttcata gtgtcgttac cgccggagag aatgttttag cttcaatgaa aagtctttca
     4741 agcgataaga taagtaaaga aaacagagac aatgtattta atttttctgc ggaaagcagc
     4801 accaaaaacg gtaaggaagc atcaaaggtt tatctgaccg gcgcttatat aagcagtccg
     4861 tacttcatat atgaccaagc taaaggcgag tatacaaggt atcagttttc cgatgttcag
     4921 atagacggaa atacaaatca gcctgtaacc gtagaaaatc ttttcgtact gaaaatggaa
     4981 acccgttata ccgaagatgc cgccaagcat gtactgatta ccactcacgg taccggaacc
     5041 ggttactata taaatgacgg aaagtacatt gaaatacagt ggagcagaga aaccgattgg
     5101 tcaaagtttg tctttaccgc aaacggagaa gaaataaagg tggcaccggg taatacctgg
     5161 atatcctgta ttccttcgga taaatatata tcaataaagt gagaaataaa tgagaaaaat
     5221 aattgccctc ttctttatac tactgaccgt acttaatctg tcttgctgtg ttaaaccgac
     5281 agaacagcct tcatttacac atgctcctac agttatgcct gaggtatctt ccacagagat
     5341 aattcaaccg acagaaagta ttccgacaaa agagccggct acaccgagtc ctactcctac
     5401 ggtcatacct cccggcagca catacataga gttttatgac ctttatgcag accttacaaa
     5461 aaccggggaa atgaaaaaag ataccattca ttggagtgaa aaaggacaaa gaaccgaagt
     5521 atttgataag cataatgtca ttacaacaat agataccgaa gaaaaggtaa tgtacctcac
     5581 attcaattta ggatatgagc atgtaagagg taataccgaa aaacttctgg agatacttag
     5641 aaacagaaat atatctgcta ctttctttct tgtaggggag tttattgaag tatttcccga
     5701 aaaagtgaga gctatttatg ctgacggtca tactttagga gcacacagct attaccatat
     5761 acctccttcc catacaatga ccgatgaaga atacatagag gattttatta aaacggaaaa
     5821 attgctgaaa attgcattgg attccgatta caaaaggatg agatattaca gaccgatatc
     5881 gggcgaatac tgtgaaaggg atttatacat tgcgaatgca ttaggttaca cgacggtact
     5941 gtttgatgcc acatattccg actggaacag gacggttata aacggatatg aatatgcata
     6001 caacgaactt gtaaaggata tacacaaagg agccatatat cagcttcaca tagtgactcc
     6061 ggacaatata gaagcattac cggtttttct tgatgaactt atcggcgacg ggtggatgtt
     6121 caaggctttt aattaataat tgacaaaaca tgtccgtatg gtaaaataca tttacaataa
     6181 agaggaaggc ttttaatgaa gagcagaacc aaaagaagac tattcacatt aatatcattc
     6241 gcattcattg tatattttat atatatcatt ttttcgcaac agccgagaat tgatatgctg
     6301 atgacaaaaa agcagaaagt ggaggaaaag ataaaagagc aggatgccat acatgaacag
     6361 ctgatctatt atcagtcgat agccggtacc gaccggtaca tagagatgat ggcaagaata
     6421 aggttaggtt atgtaaaggc cgacgacctt atattcatag atgcggacag ataagaaagt
     6481 gtaaaacagg ataatgtgga ctataagatt ttgtgacagt aataccagat atatagtttt
     6541 agtaagagaa gcactgtttg acggtgccgt tttatacagc aaaacagaag aaaccattct
     6601 gagattatgc aggaagctta ccgtagttac acaggaacgt ctcggagtta ggtgtgaacc
     6661 gtataagtac atgtccgatc ctaaaaagga gcggttttac gaggttaagc ccacaaccat
     6721 tgatatagta tacgaaagct tactcagtat tttgaatcat tagtccataa ggattagtgc
     6781 aggtgaaaag aaacaggagg aaggattcca tgaaaaaacc atctaaagga tttctaatat
     6841 tagcattagt gtttgtattg ctcttaacgg catgcactaa gcaggagcta ccgaaagaaa
     6901 cggtaagcag tgaggtgaca acaccgcctg cggaaaaccc caagattgaa acggaagaac
     6961 ctgcaaatac atatgacatt gagaagtacg atgatatggt agttgaactg tttgcattaa
     7021 aagaggataa caacggtaac tattgcgaca tgtttttgag aataaactat gcggataaga
     7081 ttatagagat acctgacaga aagcaatttg agttaagtaa acctctgatt ggaatattag
     7141 atattaacgg agatggaaat ggcgaactgt ttcttaaatt tttagaaaaa aacgagcaaa
     7201 acgagttggt tgagaatgtg tattttatgg acataaatac ggaaaaggaa ttatctcttt
     7261 ccgatttagg attggaagat acagccgagg ttacacatgg aaccgctgaa tttatagtaa
     7321 atgataaaac agtaacatat gattttaatg agttgcattt caaaggcaag gaaaatctga
     7381 acaaaattct gaagaagaaa gatatacaaa aagcaccgat gttatataca gataatctta
     7441 cattaatgat tctgtctgaa ccgatgcctt ctaccggcag aaatcaacga gtgactattt
     7501 tgaaatatga cgataaattt gctttttacc gttatcagaa tatgcctatg ccttttccta
     7561 agtacaataa cggaaaagaa gagattacat atgagagttc cgtgcagatg ggactaattg
     7621 accttgataa tgacggaatt aaagaaattg tcataaccat gtgggtagga aagggaacgg
     7681 gatattcagc tacagattta cacattatag acagcactac tatggaagaa attgttacac
     7741 ttacaaatga tgatatagca attctcataa gcagttatat tgatgcaaaa atagttacaa
     7801 aagaggagca ggttataatt aatatctgcg ttaataacga ttctgataat acatataccc
     7861 ataccgtgaa acgcaatgag aatatcacat actttgaaac tccatctttt actcaaagtg
     7921 tcggatttgc tattgatgaa acaaataaca aagtcatgat cgacgccgga atgatgattg
     7981 cagcaacttg gtatgcagga tcttttagtg cagatataac ggtggttgac ggtaaagtgc
     8041 attttgccaa tatcaataat attttttttg aatgaaaaat gaatttgaaa aatacatgaa
     8101 ataaaaaaga gtagaggaaa ttatgaaaag gctttctaat aagaacatat tactatttgc
     8161 tatgatagtt atgcttgtat tgctcttaac ggcatgcact aaaaaggaac taccgaaaga
     8221 aacggtaagc agtgaggtga caacaccgcc tgcggaagtt gttgaagact ttgtacttat
     8281 agcagagctt tcgcctaccg acaagtattt gtcacctatt ttatgtggtg taaaagtata
     8341 cggaaagaac aaggataatt acggaaacta taccgaaatt aagtttgtat tcaaggataa
     8401 agaatggatt tccgatgaat tgaaaatatt ccctcgaatg gaattaagaa gagaagatga
     8461 acatagaatg tatttaccga cattagaaaa tcgggatttt atttcggtta tttatggtga
     8521 aaaagatagt gaagacacaa ttagagagaa tataattttc tttgattggc agacttttat
     8581 gcagattaaa ccggaagagt taaatatatt aattgttcat aaacagtcaa ctgatgtagt
     8641 agtttcagac gataaatcat atactgtaac agttgcagaa aaatccaaaa atgatcggga
     8701 tatctttgat gagaaaaaga atatcatgaa atacaattac ggagaagtaa atgtatttgc
     8761 acttagaaaa gataaaatgg gcaactatta tgatttgtat gccagaataa agaatacggg
     8821 taaaaatata aaaatacctg atagaaagca atatgagtac ggaggaccga gagcttctct
     8881 gcttgacctt aacggagacg ggaatgatga acttgtatta gtgttttggg agagaaaccc
     8941 ggaaggtgta ctgacacaaa gtgtgtgctt tatggacatt aaggcggaaa aagaggttag
     9001 tctttccgat ttgggattgg aagatatagt ggaagtaaca tatcaaaaag aaaaacgcac
     9061 aatagcacac gaagatagag tgtataagga atatgatgtg gttgcatgtc atcataaaag
     9121 gaaagaggat tataacaagg acaatttaaa aacaataccc actgaagaaa taaaacgcat
     9181 atccggagat attgaccttg cattatcact tttgacagat ccgattttat tcataacgga
     9241 aaagaaaaga ccggatattt ttgtattgca atatggcgaa aagattgaac tttaccatga
     9301 ttggtcaatg cccatgcctt ttcctaagta caataacgga aaagaagaga ttacatatga
     9361 gaattctgtg cagatgggac taattgacct tgataatgac ggaattaaag agattgtcat
     9421 aaccatgtgg gtaggaaagg gaacgggata ttcagctaca gatttacaca ttatagacag
     9481 cactactatg gaagaaattg ttacacttac aaatgatgat atagcaattc tcataagcag
     9541 ttatattgat gcaaaaatag ttacaaaaga ggagcaggtt ataattaata tctgcgttaa
     9601 taacgattct gataatacat atacccatac cgtgaaacgc aatgagaata tcacatactt
     9661 tgaaactcca tcttttactc aaagtgtcgg atttactatt gatgaaacaa ataacaaagt
     9721 aacggtcgga gccggaatga tgattgcagc accttggtat gcaggatctt ttaatgcgga
     9781 tataacagtg gttgacggta aagtgcagct tacaaacatt cacttcagtt ttgactgata
     9841 atgataatgt attgagatta tacgaagcat ataatgtgtt taaataggag gagtttttgt
     9901 gtcgacaaca agtttgaata tcagagtgga taaaactgtt aaaaagcaag ctgatgaggt
     9961 ttttaacgag ttgggaatta atatgacaac agctgttaat atgtttttaa gagcggtaat
    10021 aagagaaaac ggtattcctt ttgccctgaa attaaacact cccgacagta ttactaaagc
    10081 cgctattgaa gagggtaaaa gaatcgctta cgatgattca gtgaaaggtt actccgattt
    10141 ggttgatttt aagaaagtgc tggaagagta aatttaactc acctaaaagt tccttgcttt
    10201 attggaaaac cttgttgcgg tatgttaata tagggtatgg ttttataata taaaagttat
    10261 taatgaaaga agacggttat gaaagatata aagaccaaga tagcgaagaa acttgcaggc
    10321 agcattgaac agttgagttg gacggagatt atggataacc tggagtatcc tcctgaaccg
    10381 catatgggtg atcttgcatt accttgcttt aagttaagca gagtgatgcg aaaatctccg
    10441 atggctattg ccgattcgct tgtggattct ttggatattg atgttgtgga aagatgtgaa
    10501 agtaaaagcg gttatcttaa catatttata aatagagaat atttggttaa taatgtttta
    10561 aaaaccatag tggagaaaaa agaagaaaca ggaaagagca atgagtatgc gggaaagact
    10621 gtttgtctgg attattccgc tcccaatatt gcaaaacctt tccatatagg acatttacgt
    10681 tctactgtaa taggacatgc cataagaagg cttcatgagt ttagaggata taaatgcgta
    10741 ggtataaatc atttaggtga ctggggtact cagttcggca aactcatcgt tgcgtataaa
    10801 aaatggagtt ccaaaaaggc tgttgaagaa aagggaacgg aagaactggt aaggatatat
    10861 gtaaaattcc atgaagaagc ggaaaaagac ccttcattaa atgacctggc aagacaatgg
    10921 tttgcaaggc tggagaataa agatgaagat gctatggagc tttggaaatg gttcaaaaaa
    10981 atcagtctta tagatataga gaagacatat aaagtgttgg atattacctt tgacagctat
    11041 gcgggtgaat ctttctacta tgataaaacg cagccggtag tggaaagatt gaaagaaaga
    11101 aatcttttaa tcgaaagcga cggagccatg atagtaaatt tggaagagta taatatgcct
    11161 ccctgtctca tagtaaaatc cgacggagct tccttgtacg catccagaga tattgccgcc
    11221 gcaatataca gaaaagaaac atatgatttc gataaatgca tatatgttac cgcaaccgca
    11281 caggaccttc atttcaggca atggttcaag gttgtggagc ttatgggtta cgaatgggca
    11341 aagaatctta ttcatgtggg attcggaatg gtttccatgg gcggtgcaaa actggcaacg
    11401 cgagagggaa atattgtata tcttgatgac cttttcagtg aagcatctaa aagggtgttg
    11461 gatataatca acgagaagaa tcccgatatg gaagataaag aacaaaaggc gcaggatatc
    11521 ggtgtaggtg ccgtagtatt ttccgattta tccaataaca gaataaagga tatagatttt
    11581 tcctgggaag aagcgctgaa ctttgacggt tccaccggtc cttatgtgca gtacacatat
    11641 gcaagagcat gctcggtatt gagaaaatcc gagctgtcag aaaaagatat caatatgaaa
    11701 gatattaaac cggaacatat gactaatgat gacgagttta cactcttaaa gacattgtct
    11761 ttattcaatg agaaaataga tcaggcattg agagatctgg aaccttcctg catatcccgc
    11821 tatcttattg atgtctgtat ggatttcaat aagttctaca atgcacattc catattaggt
    11881 gccgaagata acaaaaagtt cagaatactg cttacggcgg cggttaaata tgttttggca
    11941 aacggactgt ttatcatagg tctgaaaagg acggaaagag tttagtcggt tatctgaata
    12001 aacttgtgct ctcctacaga tgcatctgca aagtatcctt cttctttgtc cgacaggcag
    12061 acatacattc tgcacgcttt ctttaaagta acattgtata ttatcttttt atcttttctt
    12121 tcaaaactta ccgttatatc cgaaagggga gtaggctttg tatatgttcc cttgttaaac
    12181 tgtttgggaa ttctcggctt gaactgcaat accttatagc ccgtaccgta tgacgatata
    12241 ccgaagaata tatcattaaa aatcataggt gtactggacc acggatggca aaaactgcaa
    12301 tttttcttgt cgtccgggtg ccatgcttct atacaggttg tagcaccggt attgagcatg
    12361 gtcttccagg agtttttctc atcggaaact ataaggtcga aaattttgtc atactcttta
    12421 tggttgtata aaacggagaa atagtaatat gtgaaatata ctccgcatac tatccctctt
    12481 ctctttatat aatccaataa aggcttcttg cctttaggaa gttccaagtc gaaatacaag
    12541 gcgaataccg catcgtggaa gttgtgaata tcggaggttt ttgtaatatt gaataagccc
    12601 gtttcattat cataaaaagc gttaatcaca gctttttcaa gttgctttgc ttttttcgac
    12661 agttcctctg ctcttagctc attgcccgta taggaatata cttttgccga ttcttttagt
    12721 attccgatat atgcaaggtt gccgaaacaa ttggggcctt ttgttatctc gtcgctatat
    12781 ccgtctctgt atacagccgg ccagtctacc agcaccgtat attgttctct gtgtacatgc
    12841 tttatatgtc ccaatattcc ttcaggtcgg taaaactgag cataatatgc tattatgcct
    12901 tccaacacat tgagagaatc ttttagcgtg gatatatctt cggtatattc aaaatactta
    12961 ttcagcatga ccggaaaaac aagagaataa tctattatct gcgatgcatc gtaagtaacc
    13021 gttgtgcaga acagcccggg gcataacctt gcggagtgca tacagtcata aagaaatttt
    13081 ttcattattc tcgaatcgtt tgtataggca agatgagcaa gaccggtaac cagcccgtct
    13141 cccagaaagg ctcctttttc tcttgtgggg cagtctaaat atgattcctg cgtaccttca
    13201 ataatacctt tttcgcacat ctcccatatc ctttggaata aagagttatc cgattgtgtt
    13261 ttggaatgat tcttcggtat gggataatgt ctgtgatgca catatatgct ttttttagta
    13321 agtttacccg gataattgaa tacttcaata tatcgaaatc ccttaaagtc aaagaactcg
    13381 gctttgtctt tttcctttcc gcttaaggta attgtttctt cgtagtcgca gttgcatcgc
    13441 atattgtatc ttgcggattt attgtcttca gttaattctt ccgcatgcca tatggtaatg
    13501 acatgccctt ttttaccttt agccgttatt acggtatttc cgcatatctc ctgaccgaaa
    13561 tccagaagat aattgccctt tttctttttt ataatctcca cgggataaga aatactaaag
    13621 gaaacagtag gggttatctg cggtacaagg gtgtaggaca tggggtaagg tatcgcagat
    13681 atgaaaggag taatccaatc gcaatcttga tatcccgttt ggttccatcc gtaagggaac
    13741 aggttcaaat cgatgttttc ggcaaactgg gtctcataac cgtacacttt gtcggaagaa
    13801 taagccataa ggggataaca cttaaaagtg tggtcggaag atattctttg aacattaccg
    13861 tctttatact ctatctccaa catgcatatc atgccgttta agttatccgc agagataagc
    13921 gccatgttaa gcataccctg gtagaaaaca tggaaagaaa gagtattaag gccgggcttc
    13981 agaagtttct ttatctcaaa agcgttatag ttatacgcaa atgccagcgc ctgagcggga
    14041 ccgtatgcca aaaacttacc gttgatatag gctttgtaac agtcatccgc tgtgatatac
    14101 agttttgcac tttttatcgg aatattcttt atttcaaatt ctttacgaaa cagtgtatgt
    14161 ctgttttgca cttccgttct taagtacttg gatctttttg catcgacagg ctgtataaca
    14221 ggctgctttt tgaatatggg atcatgaagc cagaaagctt tccattcaat attcttcttt
    14281 accgatatat catacattac aattcacctc cgctatattg taacatttta ttcagtttgt
    14341 ctgtgtatta atacagttaa agaataaata atatattaat gttaatacat tatcttctgt
    14401 taatatccgc atcggtaatg ctgtccaagc tgtcggtaag accgttaaga gcattgtcct
    14461 ataccgatac tcctctgttt tacgatgata tgggctgcat gttatcggtt ttgctgtccg
    14521 tttcttttac agtatctttt tatgtaaaaa cggatattat aagcaagata ctgatggtta
    14581 tattgctctt ctgcttatgt tgcataacct ataccgactc gcaagccggc tacatctatg
    14641 acagatttca catagtcata ttgcttatag gtatagcaca actgtttata aaagggtttt
    14701 caatcactga tattcaggac agcatatcag gttgcattat agggggaggc tttttacaac
    14761 tgatgaactg tctgtcaatc ttggtattta aaaaagaagg aatagggggt ggtgatgtaa
    14821 agctgtgctt tgcatgcgga ttgatattag gacttaaagg aatgatatct gcctttattt
    14881 tcgccttttt ccctgcggcg ctatatgcct ttatatcagg cgcagtacga ggtagggagg
    14941 tggcgttagg ccctttctta tgtgtctcta tagctgccgt atattgtatt ggtatgctat
    15001 aaatggtaca atcttaaaga ggtattgaat gacgttaggg tatgaaaatg taataagcag
    15061 ccgacaggga tatatcaatt cgtggtatgt gtttcatgta aaaagcggcg aagagataaa
    15121 ggcgcgggat aaccttataa ggatatgtga aagaaaaggc atcaagctgg atttcgttat
    15181 ccctacggca attaagttca ttaaaaagaa aagacaaagg ataaaatcct acaagacggt
    15241 atttccggga tatgtatttg caaacggggt aatcgatgta gagatataca acacgctgaa
    15301 gggtataccc ggtgtttaca ggttattaag agacagaagc acttttgcgc tgctgccggt
    15361 acccgccgaa gagatggagt tacttgcggg tataatgaat gtacacggag tgatagaggc
    15421 tccggaagtg agttttagag agggacagag tgcggttatt acaaaggggc cgctgacaag
    15481 ttttaaaggt aaaattgttg ctgttgacaa gcacaagaat aagcttaccc tgctggttct
    15541 tttcctggga gaggagagaa gggtggaaat aggatttctg tttgcagaat cgttagataa
    15601 ggagaacagt taatgaaaaa gggcagtttt ttcgctttca tacacagaat aggatatata
    15661 aacagatggg ggctgatgcg caataccagt tatgagaact taaaagaaca cagctatgat
    15721 gttgctgtca ttgcacaggg acttgccctt ataggcaatg ccaagttcaa caaaaactat
    15781 gatgttaaca gaattaccgc tgcagccatg tttcatgatg taaacgaggc gcttaccggc
    15841 gatcttccca cacccgtaaa gtatgcaaat aaagaaatgg agaaggccta caaaaagata
    15901 gaaagagatg cggtgaataa tattcttaaa atgcaggacg acagcataag agaaagttat
    15961 gaacagtatt ttaacccttc cgaggaagac aggttggtta taaaagcggc ggacaagata
    16021 tcgggattta taaaatgcaa agaagaaaag gacagaggaa acaaggattt cgataaggca
    16081 tacgattctt taaagaagag catagaagag accgatcttc cggaagtcaa atactttata
    16141 gagaatatgc ttccccacta taacctggta ctggatgaat ttctggaatg aagagcgagt
    16201 aaaacgacga ttgcgagagt atttgagagc ataaaaaaga tttcgagaga tatataaaaa
    16261 acatgttgac aatacctgat atatgtatta aaatgactcg tgccgtgtaa agcggcatta
    16321 attgtggagg agaataagta taatatgaag acttatatgg caaaagccga cagtatagag
    16381 aagaaatggt atgtcgtaga tgccgaaggc cggactctgg gcagattggc aagcgagata
    16441 gccaagattc ttatgggaaa gaacaagccg gaatacacac cgtcagtgga tacgggagat
    16501 tatgtaatcg tcataaatgc cgataaggta gttgtaacag gcaagaagga aacacagaag
    16561 ttgtacagaa ggcacaccgg ttatgtcggc ggattaagag aagttgtata tagagatttg
    16621 atggctaagc atcctgacag aattatcact tatgccgtta aaggaatgct tcctaagaac
    16681 aaattgggaa gacatatgtt aaaaaagatg aaagtatatg cgggaagcga acacaatcat
    16741 caggcacaga acccgatagt tctggatttg aatgagtagc gggaggttaa gttatgacac
    16801 agtattacgg aacaggtaga agaaagagtt ctgtcgccag agtaagattg gttaccggaa
    16861 caggaaaaat aacgataaac gatagagata tagatgatta tttcggattg gaaacacttt
    16921 caatcatagt aaatcagccg ctggaagcaa caaagctcag aggcaaatat gatgttctcg
    16981 ttaatgtaaa gggcggcgga ttcacaggcc aggcgggagc tatcagacag ggtattgcaa
    17041 gagcattact ggaagctaat cccgatttaa gagcggaact taagaaagcc ggttatctta
    17101 caagagatcc cagagaaaaa gaaagaaaga aatacggtct caagaaagca agaaaagctt
    17161 cacagttctc caagagataa tatttatcga aacaatatat tacaaacagc ccgggacata
    17221 ctccgggttt tttgtatact gttttcagtt ttactgcatt ttagctcgca aatatatctg
    17281 cttttgaaaa gagattggtt tttggctatg aaatataata aaagtgtgat aatatacaca
    17341 taattctata taataatgtg attggagggg taatatggaa cgatttatat tgaaaaagct
    17401 attagactgg aagaattccc cttatcgtaa accccttata ctaaaaggtg tgcgtcaagt
    17461 cggtaagaca tgggttttaa aggagttcgg caagcgttat tatgagaata ccgcctactt
    17521 taactttgat gaaaacctgg aatataagca gttctttgag actacaaagg atgtagaccg
    17581 tattttgcaa aatctgatga tagtaagcgg tcaaactatt gtacctgaga agacgctgat
    17641 tatttttgat gaggttcagg actgtccgaa tgtaataaac tccatgaagt atttctgtga
    17701 aaatgctccg cagtatcatg ttgcatgtgc cggttctcta ttaggtattg cgcttgcaaa
    17761 accgtcttct tttcctgtcg gcaaggtcaa tttcatgcag attgatccca tgacatttga
    17821 ggaatttttg cttgcaaacg gcgatgagaa cttacttgct tttttgagaa gttgtgactc
    17881 tattgaaccg attcccgatg ctttttttaa tcctttgtac gagaaattaa aaatgtacta
    17941 tattaccggc ggtatgcctg agtccgtgtt aatgtggaca gaggcacggg atgtttccgc
    18001 tatgcaggaa gcattgtccg atattatagg tgcctatgag cgtgattttg caaagcaccc
    18061 cgacataagc gaattcccta aaatctccat ggtctggaaa tccgttccct ctcaattagc
    18121 taaagagaac aaaaagttta tctacaaggt tattaaggaa ggtgcaagag cccgtgagta
    18181 tgaagatgct ctgcagtggc tggtagatgc ccgactggtg cataaggtat atcgcagttc
    18241 cgctcccggc ctgcctgtag cagcctatga cgatttgtcc gcttttaaga tttaccttgt
    18301 tgatgtaggt cttcttcgtc gcttggcaca attggcaccc actgcattcg gcgaaggcaa
    18361 tcgtctgttt actgagttta aaggtgcact gactgaaaac tttgtactcc agactttggt
    18421 gacgcagttt gaagctatgc cccgatattg gagccaaagt aatcctccct atgaggtaga
    18481 ttttcttatt cagagagaga atgatatttt ccccattgag gttaagtctg aaacccacac
    18541 tgccggtaag agtattaaga aattcaaaga attgtttccc gataaagtca aactccgtat
    18601 atttttctct ttggataatc ttaaattgga tgatgatgtg ctgaacattc ctctttttat
    18661 ggctgacagg acggataaat tgatagggtt ggcactggaa cagcgtaaag tataaatata
    18721 gatattattt ctttcacaag ctctgtatgg ataggatttt attctgtaac tgaatttctt
    18781 cttgttctgc gtttacaaca caaaatcgga aaaagcggca aaaagccttg aaatttatcg
    18841 gaaaaaacgg caaaaacact tgatatttat cggaaaaagc ggtaggataa taatgcagga
    18901 gaggtggggc atgctaaaac gcaagatata taaacaaatt gaggatttct ataaatgcca
    18961 cagcaataag gctcttatga ttaccggagc tagacaggtc ggaaagtcat atatcattga
    19021 aaaatatgct aaagagcact attcatcatt tattcgtatt gactttattg agaatcccga
    19081 atacatagcg cttttcgaca atgtcacttc cgctaccgat gtgttgctga agctgtccgc
    19141 aatttttggc gataaaatga ttcctatgaa gactatcata ttttttgatg aggttcagga
    19201 atgtaaagaa ctcattactc aaattaaata tttagtttcc gaaggctctt atacatatat
    19261 cttaagcggt tcattacttg gaaccgttat gaacgatatt cgctctatac ctgtaggata
    19321 cttacaaata ctgactatgt atcctttgga ttttgaggaa ttcgctattg caaacggtgt
    19381 cggagagaat atatttgata tgctggaaag tgctttcaac agtcttactc ccattgacga
    19441 ttttatacac aagaaactat tatcactctt tgaactctat ctgattgtag gcggaatgcc
    19501 tgcagttgtt tcaagtttta tttccactaa aaatctaaag tttgttttgg atacgcagaa
    19561 gatgataatt gaactttata agcgggatat ttcaaaatac gataaagaga ataagcttta
    19621 tctcaataac attttcgatc tgattccgag cgaactaaat gcaaagaata agaggtttat
    19681 tctgaagaag ctgaacgaga atgccaagct gtcaatctac ctgaacagtt ttctgtggct
    19741 taaaaatgcc ggtgtagcaa taagtgtttt taatgccaat gaagctaagg ttccgcttgt
    19801 actctcaaag tcctcgaatc tttttaaact ctttttaaat gatgtaggtc tgcttgcggc
    19861 actttacggt gaaggaattc aacttaagct cttaagcgga gaactgaata ttaaccatgg
    19921 tgcaatatat gagaactttg ccgccacgga gcttacggca cacggtttta ttccctacta
    19981 tttcaatagt aaaaaaatag gagaggtgga ctttttagtg gaatcggagg gcaaggttgt
    20041 tcccattgag ataaagtcgg gcaaagacta ctaccgccac agtgcaatcg ataacatgct
    20101 tgaatgcaag aattatgata ttgaaaaagc cttaatcttt tgcaacggca atatagagaa
    20161 aaaagacaaa gtagtgtatt atccgatata tatgcttagc cttataagaa aaaaagatat
    20221 aagcgaagat attattgttg aaacggattt ttcagcactg aaagaatgat ataagaggga
    20281 tatgaagaac tgtcataaaa ccatgacgat atttcccgag gtgaaacatt ataattttgc
    20341 cgataacttg tttgcacacc ttgaacaaac aagtttatga aatgtgctaa aaacaacttt
    20401 cctttgtagt gctctgtgtt tgagcagaga ttataatacc gaaggaataa gacagaaaat
    20461 atgtaagact tttttggcta aagtttaagc tgtaagttat acagtatagc gtgcaagaat
    20521 taaacatgcc ccaagttaag ggggcataat ttgcaatata tataaactat accctcttaa
    20581 cttgacaaat gagctcgttt tttttagaat atgtgtgaag tatttgctca caaagcgggg
    20641 tgtttctatg aaattatata ggcgggagaa ctatttaagt aaaattcgcg gattttatca
    20701 tgatgtcggt atgattaagg ttattaccgg tgtaaggcgc tgtggcaagt cctgtctgat
    20761 gcagactatt gctgaagaat tgcaagaaca gggaattaaa ccttcaaata tcatttttat
    20821 agatttaaga aaacacgctt acaagtcgat acaaactccg caagccttgg aaaaaacgat
    20881 tgatgataag tgcaactccg atgagaaaaa atatcttttt attgatgaaa ttcaaaatgt
    20941 taaaggcttt gagctcgttc ttgaggcata caggtcggaa ggggaatatt ctatttttat
    21001 aaccggttca aattcttatc ttttaagtgg cgaactcatt actgctctca ccggtcgtta
    21061 tattgaattt gaaatgttta cactcacatt tgaggaatac ttggatatga aagaattcta
    21121 tggcaagcct ttaagtccga acttagttct ggagttggac aattatatta tggagggcgg
    21181 atttcctaag gctttggaat atgataatct caaggataag cggacttatg taaacagtgt
    21241 aataagcgaa attttgaaaa aagatataaa gcagcgggta aaaattcgca atttcgaggt
    21301 ttttcaaaaa atacaaactt atataatcaa taattttggt gcaactacaa gtatcagcaa
    21361 cattttgagc gaattaaaaa aagatgggct caatatcaag cgtgagactt tggcaagata
    21421 tattcagtta ctcatagacg caaaaatatt atacgagtgt aagcgctttg atttaaaatc
    21481 cagacgttca ataaggggag aacagaagta ttatctggca gacctctctt tttactttgt
    21541 gaccaatgtt gataacagaa taaactatgg tcctgtattg gagaatatag tttaccagta
    21601 tgccgcttca aaggattatg agattagcgt tggcaggata ggtagtttgg agtgtgattt
    21661 tatcgtgaga gatattgaaa tgaactatgc ctatattcag gttgccatga ctattatgga
    21721 cagcagggaa gccgaagaaa gggaatatgc cccactcgaa atgatacggg acaactaccc
    21781 taaatacctt cttactcgaa acgatttact tcaacgtaga aatggaatta aacaccgcaa
    21841 cattcctcag tttataaggg ataatgactt gtttgattaa tccatattgt ttatatatca
    21901 gtgggattat tgcttcattt ttgaataacc cgttttagtc ttgcagttgg cgtcgactaa
    21961 tgttgtagta tacttgcaca actccgcatg ggttattaaa gtatagttgc acaatatacg
    22021 caaaattatt ggggtataaa tgaataagtg tgcagggcaa tgaaaggtgg gacgctcaga
    22081 cgttttgtta tcttgtccgg tgctagacgt gtcggtaaga caactattat gtatcagatg
    22141 atagattgtc ttcttggcga aggcataagt ccgcgaaata ttttatatgt atcctttgat
    22201 aatccgattg taaagttcgt ggatatagaa acagtcattt ccggttatga gtctctttat
    22261 cccatagaag gtacaaaata tgttttcttt gatgaaatcc gatacgcgta aaactggtaa
    22321 ctttggctta aagttatata cgatagccga aaggatattc gcatgacagc aacaggttct
    22381 gcaagttcta taccggaaaa ggccgtgtca gacagcggta cgggtagcag gaactgtgct
    22441 gaagattcct tctatgtctt tctatgagta ttgtcgcctg ctgcagttag aagagcccaa
    22501 attgccggat ggtttaattc tttcggagtt tgtcaaaatg tcagatgctg aaaaaggtta
    22561 agtatcgtaa caaagcgcat aaatgatttc ggaatctcaa aacacgtaac aaaagttccg
    22621 atattgcgta tacccacaat agtatttctg tatttgacgg gaagaaaggt taattgacag
    22681 gttgtgcaat atgcaagaac tttctcttgc ttgtgtatat cccgacttcg gtataaaata
    22741 ggcgatggga gagagtatgg aaaaaacata tgtaatagcc acatgcaatg aggaagatac
    22801 gataaggttg ggtaagatac ttgcatcggt ttttgaggac agggttatag ccgtgtgcgg
    22861 ggatttaggt gccggtaaaa ctcattttgc caagggaatt gctttgggaa tgggaatagc
    22921 aaagcatgtg accagtccta cattcagcat attgaatatt tatagaaacg gtgacaagac
    22981 cttttatcat atggacgcat acaggcttac cgacgaggaa agtgcatata atgcgggaat
    23041 tgaagatata ctgcaaccaa agggaattac gcttatcgaa tgggcggaga atgtaacgga
    23101 tatactgtcc gacgggtatg taagtgtgaa tatagaaaag ggttcggacg agaataaaag
    23161 actgctgtat gtaacggcgg acagacaatc tgtagacagg atggctttgc ttatacatga
    23221 agatactgat aggtgatacc tgtttaaaaa cagcttcggc gataatagtg aacggaaaaa
    23281 agatacttgc atacatgcag tcttttggag aagagcctca ttcggttatg ttccctaaac
    23341 ttgtcgatca ggtcttaaat atagcggaaa cggattttaa ttcattggat gccgttgcat
    23401 gtgtatacgg tcccggctct tttacggggt taagaatagg gctgacatat ttgaaaacat
    23461 tggcatacac tctggatata ccgttttatg ttttaaacac tttggatgtg ctggccaata
    23521 tggaagaagg aaagagatat gttgtttcca ttcctttgat tgaagctcgg aacaatgaag
    23581 tttttaccgc catgtacgag tcggaaaaag gcatatataa aaggattacc ccttatgcag
    23641 ccaaacatat tgatgaggta gttgaagatg cggtaagttt atgtgccaag gatgataagg
    23701 ctttaatact gttaagcgga gatgctcaga aaaaacatat agctgagttt caggaacatt
    23761 tgactgataa agttttaccg tacagtgaag aaagagataa tgtggatttg agtagggctc
    23821 acctccttat atcagagcaa tgcagaaccg atccttttac tgcgcaaccg ttttacttaa
    23881 gagaatcggg agccgtaaga atgaagaaga aaaaacaatg aatatacgga taaaaagagc
    23941 ggatattaat gatgtggaca gaatattcgc tatagaaaaa gaaaccttca atgactactg
    24001 gtcgtatgaa tcgctgtatg aggatatatg cagaacaccc atgtcattct atttagcggc
    24061 agtctttgat gatgaggttg tgggatatat aggtatgtgg catgtattgg acgagtctca
    24121 tattatgaac attgcggtgg ataaaaggta tcgcaataaa gggataggaa cattgctgtt
    24181 gtccgcactt atcgactaca gcgggcaagt gggcataaag cggatgacat tggaagtaag
    24241 ggagcataat gatgccgcat taagactgta tgaaaaacac ggttttgtaa aagacggtat
    24301 acggaaagag tactacagag atacaaatga agatgccgta attatgtgga aggaactgta
    24361 aatgaccgta ttgaatgaaa aaatagctta tctgttatcg acttgtccgg aaaagccggg
    24421 agtttatctt atgaaagata ataaaggtaa cacaatatat gtaggcaaag cggtcaatct
    24481 gaaaaacagg gtgagctcct actttaccaa tatgtcggca cattcatata aaaccaaaaa
    24541 gatggtaagt aatattgaga ctttcgagta catagtatta gataacgaaa ggcaggcttt
    24601 tttaaccgaa agcgatctta tcaagtcttt gcgaccgaaa tacaatatct taatgaaaga
    24661 cgacaaatct tatccgtata taaagataac tacacaagag caatttcccg gaatatatgt
    24721 gacaagaaac agatcggata agaatgcgaa atatttcggt ccttaccccg gcagagtatc
    24781 caagaaaaac actgtgacgc tgctgcaaag cgcatttatg ttgcgttcct gcaagaagat
    24841 ggctaagcgg ccgtgcatca attacgatat gcacttatgt cttgctccct gttcagaaga
    24901 ctgtgataag aataagtata gagaagctgt ggatgcagca ttgcggtttt tatcgggtaa
    24961 cggtagagac cttattgatg aatacaaaag taaaatgcag gcagaaagcg ataatctgaa
    25021 ttttgaaaaa gccgcatatt accgtgacag aataaatctg ttgacaacaa tatccgacaa
    25081 ctatatttta gataccgacc gcaattccgc ggatattatt gcatatgcaa gaatgaaaga
    25141 ctttgtgtct ttctttatca tgaacatcag agacggcaaa gtggcggaca aaaagaccgt
    25201 aaatatacat caggagcgta tagaggatac cgatataggg tatgagttta tacgtcagta
    25261 ttacgatacc aatataccca aagagattgt tgtgtataac gaaataatga acagggaaga
    25321 tacggaacaa tggcttttta acaactgcgg aagaaaagta agtattaccg tgccgaaaaa
    25381 gggaaagctt aagaaacttg ccgatgcagc atatgaaaat gccgtagtga cattgaatct
    25441 tttcatgggg cagtataaaa attccgcagg cacgagtatg gtctattctc tggcttcttt
    25501 cttaggtctg gaaacggttc ccgatgttat tgaagctttt gatatatcgc atctttccgg
    25561 acaggacgcc gttgcatcac aagcggtatt caaagaaggt gtaccgtata aaagcgggta
    25621 cagaaggtat atcataaagg gggataacac ccgttccgat accgactctt tgcgtgaagt
    25681 aatggaaaga cggatgaaaa gaggcgatta tcccgatttg tttttaattg acggaggatt
    25741 caatcaggtg gctgcggtat gtgatatagt cggtgacaga gtaccggtag cgggaatggt
    25801 aaaagacgat aagcataaaa cccgggcatt gatatatgat gcaaaagaat acgatttaca
    25861 gtcggacaga aacctttttg tattcatttc ttccattcag aatgaagctc acagattcgc
    25921 aatagagtac aacaggaaga aaagagcaaa gaaggtatat gagtcggaac ttgacaatat
    25981 accgggagtg ggtgagaata ttaaagccgc tttactgaag catttcggtt cggtaagagc
    26041 aataaaagct gcgggcgagc aacagattgc aaaagtgcaa ggtgtaggtg ttaagagagc
    26101 aaaggcaata tatgactact ttcacaaacc ttatggaggg taaatggctg tttatgcgat
    26161 aggtgatctt catctttctt ttcagaccga caagccgatg gatattttcg gtcccggctg
    26221 gatcggatat gaagagacac tgtatgaaaa ctggcagaat accgtaaaac cgcaagactc
    26281 ggtcataata ccgggtgatt tcagctgggc tatgtatctt aatgaggcgg tggaagattt
    26341 tcgttatctt aacggtctta acggcagaaa gattctctta aaaggcaacc acgattattg
    26401 gtgggaaaca aagaccaaga tgaacagatt tttaaaggag aacggtttta ataatattga
    26461 ttttctgcat aacaattcct ttgagataga aggtgtcaat ttctgcggca caaaagggta
    26521 cgattctaaa gaggcagatg aaaagattat aaacagggag ataatacgtt tttccatatc
    26581 ctatgaaagt atcaggaata aatccgcaag aacaatagcg gtattccatt atccgccgga
    26641 aaaggaactg ctgtatatga tggcggaata taacatagag atttgcgtat tcggccatct
    26701 tcacggtttg gaaaagagca gctataacaa gcccggattt actttggtag ccgccgatta
    26761 tgttgatttt aagcctgtgt tacttatcta gtgtttttta acaagcctga taggttttat
    26821 cagttcgttt atattgtcat ttccaattcg attgccaggc acagaacaag aacaatacaa
    26881 gtcacaggac aagtcagttg aaaccttttc cgatttttga taattaaact taaatatttt
    26941 ttgcaacata aacacacaca tacattacca ttatgcaaaa attgttataa taatcgcatt
    27001 atacagttaa cgccgttaat tgtataatat attggtaaaa cccattaaaa tgcggaggtg
    27061 taatttgaat atatcacaag ttaagaaagt tgccgaggaa ataagggaga atatcggtaa
    27121 agttataatc ggtaagaacg acataatcga tctggtgctg gtatcgttac ttagcaacgg
    27181 tcatgttctt ttggaggatg tacccgggtt aggtaaaaca atgcttgcaa aaagtctggc
    27241 taaatccatt gatgcacatt ttgcacgtgt tcagttcact cccgacctgc tgccttccga
    27301 tctgacaggt gtaagtatat ataaccagaa gcaaggcgaa tttatattca agaaaggacc
    27361 gctgttcacc aatatagtgc ttgccgatga aataaacaga gcaacaccca gaacacagtc
    27421 ggcattgtta gagtgtatgg aagagcatca gataaccgca gacggagtta cctacaggtt
    27481 ggatgagccg ttctttgtaa tagctactca gaacccgttg gaaactcaag ggaccttccc
    27541 tctgcccgaa gctcagatgg acagattctt tatgagactt tccataggat atcctcagat
    27601 agaggaagag atagccatac tgactcgctt tgaaagagat atgcctctgg aggagttgaa
    27661 gagtgtggca acaaaagatg tcattctgaa agcatcacaa gccgttaaaa cggttgcggt
    27721 atcggatgat atcaaagact atattgtaag aatagtaacg gctaccagaa attccgtaag
    27781 gcttatcctg ggcttaagtc cgagaggaag tctggcactt atgagagctg caagagcgta
    27841 tgcggctatt aaaggaaggg attttgtaat accttccgac gttaaaacgg ttgctcccca
    27901 tgttatgagt cacagggtta tgcttaaagg gcactcgttg tcttacggca tgaaaggaac
    27961 cttggatgtc ataaacgaaa tattggatac ggtaccggta cccacagagg aagtatagta
    28021 aagtatggaa cttatactga ttgtcacaag ccttgtaata ttctttgttg cggagattgt
    28081 attcttcaga ttcttcgtat tgagaaaaat tgaatattcg cttagtttca gtcagagtga
    28141 agcatatgaa ggcgatgata ttttcattga tgaagttata aacaacaata aaagtgttgc
    28201 cgtgccgtgg cttaaggcgg atatacacgc atcgaaatgg ctgcagtttg ccaacaccag
    28261 ttccactatt attgacgagg agcgatgcgt atcttccggc ttcagtctga aagggcatca
    28321 gaaggtaacc agaagatggt atacgcgatg tgcaaaaaga ggagtattta ccatagtcaa
    28381 caccactctt actgccggtg atcttctggg tcttatgcaa ctatcggatg catatccggc
    28441 aaataccagg cttatggtat ttccgggagt gttgcgtctg gaggaagcct ttttctccaa
    28501 cagggactta ataggcgaaa tgatagttaa aaggttcata atggatgatc ctttcatagt
    28561 caaaggcaca agagaatatg tgcagggaga tcctttgaac aggataaact ggaaaaccac
    28621 cgcaagaatg cagaagctta tggtgaagga aaacgatttt acagcaaaaa aaggtatcac
    28681 ggtacttttc aatatcaagt cgtatgcaat ggaatttatt gaggttaagt ataaagaggt
    28741 aatagagctg ggcattaaag tggtcaatac cgtttttgaa aaaggatacg gaatgggctt
    28801 tcctttacgc ttaggtacca atgcggggat actgtccaac cccgatgaga tgatatttac
    28861 cgattcgcta ttcggccccg agcatatgag gcatcttaaa gttatacttg ctgaactgat
    28921 attgaaaaat catctggatt ttcccgattt cctcgaaaga cattatgatg atatatatga
    28981 tacgcaggta tatatagtat ccgcatatct gaatgatgag atagtggaaa aagcggatat
    29041 attgagaagg aacggaaata ccgtaaagtt tcttatactt aactatacat cggacgcgac
    29101 atcaaacagg ggtatggaag tgttcctgtt ggacagaaat acggagtttt tgaaggaagg
    29161 ggaatatcat ggcacctgaa aaacagataa gacgtaagat actgcccctt ttggggaata
    29221 tagggtcggt aggcggtctg actttcgcaa tatactatga aatatttccg gaaaccgttt
    29281 accactggct gggtttctgt tttctgatga tggttatagg aacagtagcg ggagtaatga
    29341 ttgtggattt ttacgataat aagggagagg gaatagtcgt agcggatgtt gacagagtgc
    29401 gactgtttct ccaacccgca tatatctatg cggtaatacc ttttgtatta ctttttgtgt
    29461 tgggactgat tgtctcccgc gggtttgttg aagctcttat cggttttgct cttatgccca
    29521 ccatatactt catattgggt ataggaaacg gtttattcga ctacacccag accgccaaca
    29581 gaaagatatt gtataccgca ttaattccct taattattgc tattttcaaa gcttttgttt
    29641 cgagccctta cggtttctat aaagatcctg tctggatatt cgggtcgctg tatatgttat
    29701 ttttcatgct tacgataaac agaattaaga tagaggaatt gtttgttctg tccaaaaaag
    29761 taaatgtgga gaataagaaa agtataagaa gaagaaatga ttttctcatt atgatattct
    29821 ttgcacttta cttgataata ttcgttgtac gtaatacatt caccagaata tcggaatgga
    29881 tattcagaaa taccgtagcc ataataagct ttataatcaa ctttttttat aaaatgcgta
    29941 aggttattga agtggaacag gttcaggagg tagtcgagga aggtattcct gacgaagtac
    30001 ctccggtgcc tccgacaatg aatccgacgg caaaaacaat ccttatagtc atagcttcac
    30061 tgactatagc ttttgtgttg tacttcctgg tcaagaagat aattcagctc ataaggtggc
    30121 tcataggcaa agcaagcgat acattaaccg gcggtaagag aataaaaaga gatacggaaa
    30181 atgaagagtt tgaagagatt gtggaattta ccaaagataa agcacgaaga aaaaggcaaa
    30241 aagtcattaa gtataagtat tcgctgagag aacttaggaa tctggataca tataaagaga
    30301 aagtccgtta tctgtacggc ttcgcattgg aaaggctgaa atacaagaat gtaaaaatat
    30361 ccaaagcaga cacaccggaa gagattgtgg ataagctggg cacttatgag aacggaaaca
    30421 aacttgtcga taacgtgttt aaggatttta ccgacgagta tatcaatgtg cgatacggca
    30481 ataaagaaac ggggcaggat gagaatttca tggaaaaagc cgatgccgtg gacaaaggca
    30541 ttgaaggcat aaaagtcaag gaagaaaagg caacagcctg actttaatct tattgcatat
    30601 tttgcaaaac atatgtatgt ttgatagaat agcagatgcg caaaataagc tgtgaatgtt
    30661 ttacggcttg atgaggaaga atgatatgac ggaaaaaaag aataatatag aagaaataat
    30721 tgaaaatgaa gatataaagg aaaaccctat gaaagtggag tttaccaaga taccccttat
    30781 accccttagg gccgttgcgg tgatacccgg tatggttacc catttcgatg taggcagaga
    30841 gaagtccatt cttgcactgg aaaaggccat ggaagaaaac ggacaggttt ttcttgtaat
    30901 gcagagagat gcttcgatag agaatcccaa gcgggaaaat atgtatgaat acggaaccat
    30961 agccaatgtc aaacaggtgt taaggcttcc gggaagaacc tgcagaatac tggtggaagg
    31021 tataaaaaga gccaaactgg aagaaaccgt tacggtcaag ggttactata cggcaatagt
    31081 caccataccc aaaattacgg aagcgatcat atccgcaaac aacattttgg aagccatgca
    31141 cagaaagctt accaccttat acgaagagta tgttacattg ctgggcagaa tatctcatga
    31201 aggatatttc aatgccgtgg ctgcggatga cataggtgtg gttgccgacg caatggtggg
    31261 ttccatggcg ttgaaatcgg aagtaataca gtcctttatc gaagagttgg acggaataac
    31321 tcgtgcggag aaactgcttc ctcttctggt taacgagatt gagatactta aattggaaag
    31381 agatcttgcc actaaggtaa aaagcaccat agacgaaaaa cagaaggaat attatcttcg
    31441 cgagcaacta aaagccatac aggaagaact gggtgaatac agcgaagaag tggatgaaga
    31501 cgagatgggc gaatacatca aggcgattga atccaaaaga tatcccgagg aagtcagggc
    31561 aaagctgaaa aaagagatga gcaggctttc caaaatgtct cccatgtctc ccgaagcttc
    31621 ggtaatccgc tcatatatgg aaacgctcat agagcttccg ttcggtattg agaccaagga
    31681 aaagctggat attaagcatg tcggaagaat actggagaga gaccattacg gcatgaataa
    31741 ggtaaaggag aggataatag aatacattgc ggcaaagaaa ttgagcaaca gtttcaattc
    31801 tcccatatta tgccttgtgg gacccccggg aaccggtaaa acttccatag ttaaatccat
    31861 agccgaagcc ataaatcgta aatatgtacg catgtcctta ggcggcataa aagatgaagc
    31921 ggagataaga ggacacagaa gaacatatat agggtccatg ccgggaagag tggtaaaagc
    31981 cataaaacag gccggttcca tgaatcctct gatactgttc gatgaaatag ataagttggg
    32041 aaatgatttc agaggagacc ccgcaagcgc catgctggaa gtattggatg cagagcagaa
    32101 taacgctttt agggaccact atctggaggt atcgctggat ttaagccatg tgatgttcat
    32161 aaccaccgcc aatactgtgg aaactattcc ccgggcatta ctggaccgta tggaggtaat
    32221 agaggttacc ggatataccg aagaagaaaa aatgcatatt gccaataagt atttaattcc
    32281 caagcaactt aaaaagcacg gcatgaccag gaagcatctt aacattaccg attccgtgct
    32341 gaaaaaagtc attagcaatt acacaagaga atcgggagtg agaaatctgg agagggagat
    32401 agcttctctg tgcagaaaag ttgcaacaag aatgcaactg gaggaaaccg acagagtaaa
    32461 gctgacggtg aacaatattg aggatttctt aggaccgcag aaatatcatt atgacacaac
    32521 attcgacaag gacgaggtgg gaattgccag agggcttgcc tggacacagg tgggaggaga
    32581 tacccttact atagaagtga atataatgaa cggtaccgga catgtggaac tgaccggaag
    32641 gttaggcgat gtcatgaaag aatcggccaa agccgcaata ggatatatcc gttcacagac
    32701 tgacaggttg aacataaatc ccgatttcta taaagacaaa gatattcatg tacatgtacc
    32761 ggaaggtgcc gttcccaaag acggaccgtc ggcaggtatt accttggcta ccgcaattat
    32821 ttccgctctg acaaacagga aggtcagaag agatgtcgcc atgaccggtg aaataacctt
    32881 aaggggaaga gtacttccca taggcggtct caaagaaaaa ctgtccgcgg cgcgaagagc
    32941 caatgtacgg caggtcctta tacccatgga aaatatacgg gatttggcgg aagtacccaa
    33001 gcagataacg gacgagttgg aaataactcc ggtcagcaat atggatgatg tgctaaagaa
    33061 tgctttggca gaatagtatt aaataagtta ctgatgaaaa acggcgcagg cttagggttt
    33121 ttgaatcttg agcctgtctt gcttccattt gccggaaaag taaaaatatc cccctataat
    33181 catggaaaag caggtggata tgggaacggc atatcctaag ccgcccactc ccgtttcaaa
    33241 aaccttggca aaaagtaaga ccaacggtat cctgaacacc aaggagctta ccatggcgga
    33301 agtcatggaa ataattgagt atccggctcc gttcagcaga ccgttaaaac agaaggcgaa
    33361 aggcacaata agatagtcca gactgaatgc cttgatgtag gcaacaccta tacttacaac
    33421 atccggttcc gctttgaatg ccagaagaat ggattcggga aatacttgta caaaagtgaa
    33481 tatgacaatg cttatggaaa aagatatggc tatagcggtc caaagagtct tggatgctct
    33541 gtcatacagc cccgccccta tattctgagc agccatagcg gaaaccgatg cactcatggc
    33601 tgcggcggga agaatggcaa aggaattgaa tttacctact atacccactg cagcggaggc
    33661 tgtcaccccg cctatatcat ttatcatgga tgtcagcacc aaaaaggaaa tacccacaac
    33721 gacactttgt gcactggaag gaatacccac tttcagcagc agtttcagtt tatctttgtg
    33781 tattttaaaa ttttcacgac ggaactcaaa tatgaatccg cttcttttaa gataaataac
    33841 agccaatatc atggatatag cctgggatat tatggtggca agtgcggcac cggcagcttt
    33901 catgcccaga ggacctacaa agaggatatc cagcacaaca tttattacac atgctatacc
    33961 cacaaacatc aaaggtcgct tggaatctcc catacccctt aatatcgcac ttattgcgtt
    34021 ataaccgaaa acaaatatga tgccccacat gcatatgagt acataatctt ttgccatgga
    34081 ataagattct tccggggtat tcaacacttt tagcattaca tcaacaaaga aggtcatgaa
    34141 gattgaaaag aagattgcta aaagtcccat taccgaaaac agagtgccta tggttttatt
    34201 aacttcctcg tgcttctttg cccctatata ctgcgctata agcaccgtac ctccgattgc
    34261 cagacctata acaaagttgg ttactatcat ggtgacctgt gcgcctatgt ttactcccga
    34321 tatggtgtat gtaccgctta accatcccac aataaacata tccgcaacgg aatacaaggc
    34381 ctgcacaata ttggaaagca gcaacggaag actgaattta atcagctgtt ttgccacatt
    34441 tccttcggtt aatctgtttt caaatcccat aatgcctccg tttatgcaat ggtgtaatgt
    34501 tagcataata tattcttgat aacaaggttt ttgttaccga gccttatgta ttacatttac
    34561 aaaaagtgtg tttaaatata taattaccct aatcagcagg gaaggagtat gtgacaaaga
    34621 aaaaacatgt cgcataaaag gtattatgag aaccattgcc gagataaatg aaaagattaa
    34681 aaaaggcaag gccgttgttg taacggcgga ggaagtaata gacatagtcc aagagaagag
    34741 ctataaaaaa gcttatgaaa caattgatgt ggtgactacc ggaacattcg gtcccatgtg
    34801 ctcatcgggg gtgttcataa acttcggtca ttccaaccct cccatacgaa tgcagagcat
    34861 cctgctcaat gatgtgtgtg catacggcgg tcttgcggct gtggatacat atataggggc
    34921 taccgaagtg tcgcagaaaa gaggcatgga atacggcgga gggcatgtaa tagaagattt
    34981 gatagcgggt aaagacatta aactgcatgc gctgtcttcc ggaaccgatt gttatccgag
    35041 aaaagaaata ctcacaacca tcaacaagga taacataaat caggcgtata tgttcaatcc
    35101 cagaaacgca taccagaatt atccggcggc taccaactcc accaataaga ccatatatac
    35161 atatatggga acgcttcttc ccaactacgg caacataaca tacagcacat cgggtgaatt
    35221 gagtccgcta ctaaatgatc cgtatcttcg gaccatagga gtgggaacca ggatattctt
    35281 gggaggcggt gtaggttatg tggcatggca gggtacacag cacaaggttg acgtggagcg
    35341 aagtgcaaac ggtattcctt tagtacatgc ggccactctt gcggtgatag gggatataaa
    35401 gggaatgagt acggaatatg taagaggagc ggtgttcgac aaatacggag taacgctttt
    35461 tgtaggagta ggagttccca ttcccgtact ggatgaagag atgcttaaat atgtatcggt
    35521 taaaaattcc gagatatata caaaacttgt agataaatcg ggtaatgtca acatagataa
    35581 gaaatacaat tatgccgaat tgcgaagcgg gactgtaaag ttcaacggaa aggctgtcca
    35641 tacggcacct ttatcctctt tggatgtggc acgtaagatt gcctgtacat taaaggaatg
    35701 gataaacaga ggtacttttg agttgacgga aaaagtagcg gaccttcctt gtgaaagtgt
    35761 tgttcatccg ctggaaataa gggagataaa gtaatgagca aacttaaact taaactttca
    35821 tttgataaag actgcgttac aaaaccggta atatacgaac tggtcaagaa atatgatgta
    35881 atgaccaata tattaagtgc caatatagaa tacggtaaca gaggtaatat gattctggag
    35941 ataaaaggca aagaagagaa tctgagcctt actttggagt atttcaagtc tttgaatatt
    36001 aaatatgaga tatatgccga agcaatattg tgggatgaat cgaaatgcat aaactgcgga
    36061 agctgcacag cggtgtgccc gacacaggcg cttacaatgg gtaaagacga tcttctgcat
    36121 tttgacaagg ataaatgtgt agtctgccag ctttgcatca acgcatgtcc catacgcata
    36181 atatcggtgg aattgtagag gtgtgatgta tcagccgaga gattacagaa agtcctttac
    36241 cgaccggttg aaatggtaca atgtctgtgt ggaagaaaca gacctcatga taggtaccga
    36301 tgaagactgt tcggaactgg tgctgaaaac tgtaagaact ttgagaaaag aaatatatgc
    36361 cgctatccga gacaacagtg agtttttaac atcgctggtg cccgttgaat acaaaggtga
    36421 aggtgttgta gaagccatgg ttgaggcggc taaacttgct gaagtgggac cttttgcggc
    36481 ggtggcggga gcgataaacg acatgcttgc agaaaaactt atggataaga gtaagcagat
    36541 aattatagaa aacggcggag atatatacat taaatccgac caagacagat tgataggcat
    36601 atatgcgggt aactcggttt tatccggtaa gtttgcaata gagatacaaa aagacctttt
    36661 tcccgtcggg gtatgtacct cttccgcaac tgtgggacat tccttcagta tgggaaaggc
    36721 cgatgcggca gtagttataa gtaaaacctg tgcagtggcc gatgcctttg caacggcatt
    36781 ttgtaacagg ataaaaaaga ccgaagatat tgctccggca ttggaatggg taaacggttt
    36841 tgatgaggtc ataggggctg ttgcgataat aaatgataaa ataggtgcaa taggaaatat
    36901 taaactggtg aaatggaact gatatgagaa caacattaca acaagcggac aaaatagtaa
    36961 ttaaagtggg aaccaatacg cttactcacg ccaacggaaa gatgaatctg aggagtatgg
    37021 aacttttggt ccgggagatt gccgagttgg tcaatgcggg cagaaaagtt atattagtca
    37081 cttcaggtgc tgtaggagtg ggattgggta agttaaacct tatgaaaaaa cccaggaatc
    37141 ttgctgccag gcaggtttta gcttctgtcg gtcagtgtga gcttatgcac atatattcca
    37201 agcttttttc ggaatattcc attactgtag gacagattct tcttacccgt gatgtaacgg
    37261 aaaatcccgt gaaggcggaa aatgcgtata atactttcaa aggtcttatg gatatgggca
    37321 ttgtacctat agtaaatgaa aacgatgcca tctctaccga tcagctggca atagtggatt
    37381 ccttcggcga taacgacaca ttatcggcaa tggtggcaaa acttattaaa gccgaccttc
    37441 tcataatact ttccaatatt gacggactgt atgataaaga tccgaacata aacgaagatg
    37501 caaagatagt ggacacggtt accgagatta acaaagaaat agagtcctac gcttccgatc
    37561 tcacaagttc tttgggcaga ggcggtatga agacaaaact tacggcagcc aagacctgtc
    37621 tggagaatga tataaccatg gccattatca acggagataa catgaagaat atctccgctt
    37681 tgctggacgg agataacatt ggtacactgt ttaccaacaa acgataaagt cggaggaaat
    37741 atggcataca ttgatgaatt gggaataaaa gcattaaaag caaaagaaca gcttatacaa
    37801 atagataccg aaaagaaaaa tgccgcttta agactgatgg caaaaaatct cttaaagcac
    37861 aaagaagcta tcaaaaaggc aaatactgat gatgtaatca atgcgaaaga aaaaggcacc
    37921 aaagaagcat tgacagatag acttatcctt aatgatgcca gaattgaaag catggcaaaa
    37981 ggacttatgg agatatcaca atttcctgaa ccgataggca atgttctgta tgaaagagta
    38041 ttacctaaag gtatgaaact taagaaagta tcggtaccta tgggggttat aggcataata
    38101 tatgaatcgc gtcccaatgt cacggcggat gctgcagctc tttgttttaa agccggaagc
    38161 gcggtcatat tgagaggcgg ctcagaggca ataaactcca ataaggctat tgtagcggct
    38221 ttaaaagacg ctttggaaga gatcgggata aataaagaca gtatacagct ggtggaagat
    38281 acaggaagag ataccgctac tcaaatgatg aaagccgaca actatataga ccttttgata
    38341 cccagagggg gagccggcct catagcttcg gtaatacaga atgccaccgt accggtaata
    38401 gaaacgggta ccggtaactg ccacctctat atcgacagca tttttaacga aaagtttgca
    38461 ctggatataa cggtgaatgc caagtgttcc cgtccgggag tgtgcaatgc cattgaaacg
    38521 ttgttggtgc ataaagatgt gtataaagat atactgccga aaatagaaga agagctttca
    38581 aaatacaaag tgcagttaag agcctgtgag caatcagtaa aggtattaaa gaatgcaata
    38641 cctgcaaccg aagctgactg gtctgaggag ttcttggact atattcttgc cgtcaaagta
    38701 gtgaatgaca tagaggaagc catagagcat ataaataaat acggtacaaa gcactccgaa
    38761 gccatagttt ccgatattga agagaacagg aacaagttta ccaagaatgt cgatgccgct
    38821 tgcgtatata caaatacctc caccagattt accgacggag gcgagttcgg attaggagcg
    38881 gaaatgggta taagtaccca gaaactccac gcaagaggac ctatgggtgc taacgaaata
    38941 accacatata aatatatagt agaaggtgac ggtcagataa gataatcaat agccgtctat
    39001 gggtaaatag ggtaactcgg ataaataggg caataaatcc tgctcacagc ttatgatttt
    39061 tccgtctatt tcaaacggta cgtttatacg aaacatctgc ccgctgttat ataacctgaa
    39121 agattctttg tctttggcaa aaggagcgga tactccgcat attttaggcc agccttcctc
    39181 cttacatatt gcatagagaa aaggtttcat catacatatt tcatctccgc ctatatattc
    39241 ccttacttcc acaccttgtc cttttttgaa aacacagtcg gcataggata caaccttacg
    39301 gtcttttata aggaaatatg tatttataag ttcgcttcgc acccatttat cgtaatactc
    39361 gtcattgcgc acatatacgg gacataattt gtgagcatgt tcttcaaaaa gacttttaac
    39421 tgcgtctata tcaacaggtg aggattgcac aacggaatag tgttccggta ataccgactt
    39481 tctcaatctg tatactttgt gacagaagcg aagagaaaac cagcctaatt tttcgtaatg
    39541 atgagtgtta cctgtagtaa gcctggaaat cggcatgtcc tgcttctcca tataatctat
    39601 ggccatggtt aagagttttg tacttaatcc ttgccctctg cgagaaggct ttgtacaaac
    39661 ctctcctatt cctccgacct ttattacatt gtcctttatc cttaagtatc tggtaaacac
    39721 ccttaccgaa cttactattc cggttgggtc tttggcaata aaaatcccgt ttatatctct
    39781 gtaagggtca ttcatgaagt gacgaataaa atatgcctca ccgacaggaa aaatgcaacc
    39841 gcagaaagca gtccactctt taaattcatc ttcttttaat gttcggaatt ccattgttac
    39901 ctcacttaac ggttttttgc gcataccttt atatacccgc taacagaatc atagtggaaa
    39961 gttaacggaa aaacaataga aaaacaaact gcacggtttc ccgtgcagtt tgtctgtgta
    40021 actttatatt ctaatcagtt aacggtcagg ttaatagcca atgttttatc ggcacttgcg
    40081 aaatatatct taatctggca tgtacctgta gctaccggag taactattcc ggttgagttt
    40141 accactgcaa ctgaagtctt agtactcata aatgttttac tgcccgcaat ggctgcaaag
    40201 gccatgggta ctccgtattg gtcttttgca tctacatatg cggtaatatt gaagactccg
    40261 cccgacaagg ctttggtaac agtgggttcg gttgcagcta aatgtgtaag tgtgggagcg
    40321 gcacccgatg cggttacatt caaattggca atttcctgtc cggctttcca cagtttgatt
    40381 gtagttgtca tggtctgagc tacattctgt gctttcagag tcttgacacc ggaaacatct
    40441 tcgataacga agttattggc tccggaatcg tttgtgcagg ttatctgatc tattcccgaa
    40501 ggaagggtac ccgtgtatgc aagattaacc gatttcccgt tggccagcaa tcccgatatt
    40561 ttaataggtt tccaatggcc cgtatcggta gttgtatcgg tagctccgcc gtacatggtg
    40621 ggtatatcct cgattttgta actgactatt ttgtcatcat ccactaccaa taccgtcatg
    40681 tcgtatgtat taacggtcga agctcctttt ttgagagtga aagtcaatgt atatgagcct
    40741 gaagttgcag gtgcactcat tgtgtatccc gctcccgtca tggtaccttc ggtgaacttg
    40801 gcgccggaaa tattcgaaga agtaacaata accgaatgtg ccggagtaag agctatttct
    40861 ttttcatatt ggtcggctat cttcagcata tccgcagcca ttatgatatt ggagtttttc
    40921 tgataataaa tgggcatggt tacatcggta atatatgtgg gaaccggagc ttccagtacc
    40981 tgtacatcca aagtgccctg agtaataccg tcataggaaa tatgcagggt tgccgaacct
    41041 gatgagtttc cttttatctc aatcttcttg gttgcaccgt tgaactgtat gttggtattg
    41101 ggtacaaccg tcgtattggt ggagaacatc atcagtttag tagggtcaag agtatccaat
    41161 gttgtaggat tgccgtactg atcttttgcg gtaactacaa gaggtaccca ctgatttaca
    41221 aataccgtgc cctccactcc tgttattgta agagtcttta cggcggcagg tgtcttaacg
    41281 gtaatctgta tagaagaaga tatattctgt cctacagccg ttaatgtaac ggtaccttcc
    41341 ttggcgccta tggctcctat agggaaattc agttttccgt ttgcgacggt tatcttggaa
    41401 gcgtcgatta tggcggaatc gttggatgtc agtattaccg gcttgttcag tccgatatcg
    41461 gaaggcacaa tggtgtatgc ctgattatac tggtcttttg catctaccgt aagggaatgc
    41521 cccgaagagt tgacaagaat aacggtagtt cccgatgcca gttgtacctg tccgaaagca
    41581 aaggtagtta tcttaggcga ttcgcttaat gttacggatt tggttaccgc agttccggta
    41641 gtattctccg ctattacaat attgaccgca tcattggctg caagtccttg agctccttta
    41701 agaatgaagc cggcagctgt tttgtttaaa ttgagagtta ccgaaggttt tgcctgacat
    41761 accgcactta atgtgaagtt tgtgtatgcc gtcagaggca tttcccctcc gtattggttg
    41821 gtaaacacaa ctttcaaatc ctggttatcc atagggaaaa catattgtgc ggttatactg
    41881 atagcggtaa gtttttccgc ctgcacagta aatgttttac ttgaatcgga tacggtaacg
    41941 gtatattcgc cggctgcata tgcggcagtc cttgttatcc ttaccgaacg tctgtctgat
    42001 gcccagttta aactggtacc ggtaacattg attcctccgg ttttcaacac tacctgagca
    42061 cctgcctgag cgggtgcgct gaattccacg gttacttcct taggacccga tgcataggca
    42121 ttacctatac cgaacggagc cgcagagggt gtcggagtag gagttgccgt aggcgtaggt
    42181 gtaggtgtcg gtgtagccgt aggagtagga gtaggcgtag gagtcggagt aggtgtagcc
    42241 gtaggagcag gcgttgccgt aggagtagga gtcggtgtag ccgtaggta
//
LOCUS       DAOSOM010000018        41816 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_985_length_41816_cov_5.543545, whole genome shotgun sequence.
ACCESSION   DAOSOM010000018 DAOSOM010000000
VERSION     DAOSOM010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 41816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41816
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_985_length_41816_cov_5.543545"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /gene="nusB"
                     /locus_tag="PLF14_05620"
     CDS             complement(<1..320)
                     /gene="nusB"
                     /locus_tag="PLF14_05620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOT70808.1"
                     /translation="MGRRKTREHALRLVYQIQINDDDINEQMEQYFLDNDVKNTAKEY
                     IKKAVTYINENCKDIDSVLSMYLAKNWRIERIPKIELAIMRLAYYEILNLEDVPKSVA
                     INEAV"
     gene            complement(338..871)
                     /gene="amaP"
                     /locus_tag="PLF14_05625"
     CDS             complement(338..871)
                     /gene="amaP"
                     /locus_tag="PLF14_05625"
                     /inference="COORDINATES: protein motif:HMM:NF033218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HOT70809.1"
                     /translation="MKFFKKGRKFFYTVNIILIVVLCAVTIHACLDPMDYERAFEDFV
                     DARFFSVQRGVIFLVALLILVFEAFFIVSLLRKEEKPVETVNLTTPTGQISITAYSLE
                     TMVKSIVSRFDSVTDVRTKVDFSNSQVGMDISVKVKSSVLIPEISSKIQSIVKEEVLK
                     STGIELHSIRVFVDGIN"
     gene            complement(868..1257)
                     /locus_tag="PLF14_05630"
     CDS             complement(868..1257)
                     /locus_tag="PLF14_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004396361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOT70810.1"
                     /translation="MADDVLKINGLETSLGQLKVTNEVIAILAGIAAMEEDGVYAMSG
                     GITGELAQALGIKNLSKGVKIETDEEGIHVNIYIIVELGVRIPEVAWSIQEKVKKLVE
                     KMTGSKVTEVNIHVQGVNIHKPGEEQK"
     gene            complement(1400..1864)
                     /locus_tag="PLF14_05635"
     CDS             complement(1400..1864)
                     /locus_tag="PLF14_05635"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT70811.1"
                     /translation="MDYTIRKLDAESFMCIAELRGYAKDDEDFIERYNRVVDKKDDCY
                     AVFVNDECIADVTLTYVSSDKAAAEENIRAYMSGLLVKEDYRNKKIATALIMHLIEEL
                     KEKGYKELSVLSDTDNVVASKLYAKLGFQRENTYDVEDMSFDLLVYRINRAL"
     gene            complement(1866..2942)
                     /locus_tag="PLF14_05640"
     CDS             complement(1866..2942)
                     /locus_tag="PLF14_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOT70812.1"
                     /translation="MFSKRVDCMRKIMEEENLDGAVITSRDNTLYLSSFSGSSSILIF
                     TRDKAYFATDFRYIEAAELISAYGYEVVLIQEGMKSVLSLLSKLFTKSELRIGFEKTV
                     SYGYFEALKKGLKDCEYIDVSDRLLAVRSVKDQKELSLMAKAASIADIAFTHILNTVK
                     AGMTEIEVAAELEYVMKKNGASFPAFETIAISGKKTSMPHGVPDHKKLMNHDPVTLDF
                     GANYKGYCSDMTRTFFLGSPSPKMKKIYNIVYDAQKSAQDYVREGVTGADVDKIARDI
                     IGNEGYGKYFGHSTGHGVGLLVHEEPRLSTQSTTVLKSNMTVTVEPGIYIPDAGGVRI
                     ENTVVVQEDGCYTLNHSNKELIII"
     gene            complement(2951..3802)
                     /locus_tag="PLF14_05645"
     CDS             complement(2951..3802)
                     /locus_tag="PLF14_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOT70813.1"
                     /translation="MIRFGPAGNEPSFYEQGYKNTFEAMKWLQDMGLNAYEYQSGRGV
                     NIGEQAALKIGEHAKKHDIALSLHAPYFINMATKEPDKVQSSLMHMHKSYIAAKQMGA
                     TRVCVHTGSPMGDTRENAIKRAHIFLKNFLDDIDDQSVIFCPELMGKVNQLGNLEEVI
                     YLCANDERILPTIDFGHLNARTHGTLKTPEDFERVVLKLINGIGLERTRIMHVHFSQI
                     EYTQGGEKKHLTFEDDKYGPYFQDLIPVLYKYDMKPVIICESDNTMATDALKSKIMYE
                     QYMKKDL"
     gene            complement(3799..4302)
                     /locus_tag="PLF14_05650"
     CDS             complement(3799..4302)
                     /locus_tag="PLF14_05650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HOT70814.1"
                     /translation="MYKYLPDYIAQGISDIDYKRLYDMGIRGLCFDIDNTLVGMYDEE
                     IHKKALTLINNLKNMGFSICILSNASKKRTALIATGFGLEYVYRAFKPLKQGFLIVLR
                     ILDLEPHKCAMIGDQLFTDIRGGKKAGFLTVMTKRMNNHESLYVRFKRIFEDRIMRKY
                     IKGIEKI"
     gene            4397..5461
                     /locus_tag="PLF14_05655"
     CDS             4397..5461
                     /locus_tag="PLF14_05655"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOT70815.1"
                     /translation="MNISINLTKKPKTKPDESKLSFGKQFTDHMFLMNYTPEKGWHNA
                     RIEPYGPLSLDPSTNVLHYGQGVFEGLKAYRTKDNKVLLFRPDENAKRLRASCERLCI
                     PPIDEADVVQAIVTLVKTDIDWVPSAPGTSLYIRPFIISVDPFLGVHPSYTYLFMIII
                     GPVGPYYPGGLNPTSIYVEDFYKRASKGGMGHAKTMGNYAASLIAQEKAIKLNCIQTL
                     WLDAHEGKYIEEVGAMNVFFVIRDKIVTPELSGSILPGITRKSTLEIAEALGYRTEER
                     KISVDEVIKALKNGDMKECFGTGTAAVISPVGELVYKDVRYVINDNKIGKISQKLYDT
                     LTGIQYGIIKDTRGWTLEVK"
     gene            complement(5488..6552)
                     /locus_tag="PLF14_05660"
     CDS             complement(5488..6552)
                     /locus_tag="PLF14_05660"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF017509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOT70816.1"
                     /translation="MKKSKINYTKEIRIFSVITVTMLLLTILLTGCAPYKYSRYIELA
                     EYMNLQLDYNVIAVTEEDLQKETDILLDKYAKTVVLDRGEVKSGDIANIDYSGLVDGR
                     PFAGGAETGYDLAIGTHTFIEGFEEGLIGAVIGQTVVLNLTFPVNYKDKDNSVSALAG
                     KEVVFNVVVNKVTRRVPPQYTDEFIKSLDSEYKTVEEYNEYLKKEIFKYKKTQEAWKT
                     VVNNTVVIKYPDTVQKRVDEIREYYEYYTIYYGYSSFNDFCIDALGQTEEEFNRINLE
                     SARDGVKQEMISQAIAGLEKIKLTKSDLDKGYANYAEYYGYDNTQDLFKDYDKNSITS
                     SILLNKVMEYVAKHAVVVNN"
     gene            complement(6572..7186)
                     /gene="yihA"
                     /locus_tag="PLF14_05665"
     CDS             complement(6572..7186)
                     /gene="yihA"
                     /locus_tag="PLF14_05665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOT70817.1"
                     /translation="MLVKDSKFIISAAKAEQWPDDELPEVVFSGKSNVGKSGLINALC
                     NRKNLAYSASAPGKTRLVNFYEINGVMRMVDLPGYGYSKASKQETKLYGKLAERYLTQ
                     RKNIRLIIQILDIRHDPTEDDLHMIEFLNTIGLPFVIAVNKCDKLSKSQAINRARSIA
                     GMLDIDPDTPFILTSVTAKRGMEEIWDAIDFYCFDQEDDNNETE"
     gene            complement(7299..8237)
                     /gene="fba"
                     /locus_tag="PLF14_05670"
     CDS             complement(7299..8237)
                     /gene="fba"
                     /locus_tag="PLF14_05670"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HOT70818.1"
                     /translation="MALITSKKMFEKAYKGGYAIGAFNVNNMEIIQGITEAAMEVQAP
                     LILQVSAGARRYAKHVYLMKLIEAALEDTGLPICVHLDHGDSFETCKSCIDGGFTSVM
                     IDGSHLPLEENIKVTRQVVEYAHAKGVVVEGELGRLAGVEDEIKVKKEDSSYTDPDEV
                     EEFVSKTGVDSLAIAIGTSHGAYKFQGEAKLRFDILEEVQRRLPGFPIVLHGASSVIP
                     EFVEKINKYGGKMPGAQGVPEEMLRKAAGMAVCKINIDSDLRLAMTSSIREYFALHPD
                     HFDPRQYLKPARENIKQLVKHKLVHVLGCDNTAGEY"
     gene            complement(8251..9501)
                     /locus_tag="PLF14_05675"
     CDS             complement(8251..9501)
                     /locus_tag="PLF14_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOT70819.1"
                     /translation="MTMLKGAAIFGQSGGPTAVINSSASGVFQQALKEQCITAVYGAA
                     HGIKGILNEEFYDISKESKEQLELLKTTPSSALGSVRYKLKNADVDDTDYKRLLEVFR
                     KYNIRYFFYNGGNDSMDTCNKVSKYLADAGYECRVIGVPKTIDNDLAITDHCPGYGSA
                     AKYVATTIMEVYHDASVYDTPMITVIEIMGRNAGWLTAASLLAVYKGAGPDLIYLPEV
                     PFTIEKFLSDVKKVYDSKPDHKVIVAVSEGCKDKEGRYIAEMGQSLKKDAFGHSQLGG
                     AAAILGEIVEEQFKCKVRSIEFSLMQRCAAHIGSRTDIEEAFLAGQQAVKAAVEGHTD
                     YMIGFERTNEKGKYGIKTVLIPLAKVANAEKTVPLEWFNQEKNFLKKDFIDYALPLIQ
                     GDSGLPFEEGLPKFAKLKKVSARK"
     gene            complement(9580..10125)
                     /locus_tag="PLF14_05680"
     CDS             complement(9580..10125)
                     /locus_tag="PLF14_05680"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOT70820.1"
                     /translation="MEHESKAADKVLTLFHKMKIRSEKKRSLEDAETDPEKIKRIKAK
                     RETLQAIKYFIVAGSAGAIQVGTYALFHDAMKWVPWLAHLVSLILSVLWNFTLNRKYT
                     FKSAVNVPKAMLKVALYYVVFTPLSMFWTGALTTGIWGVEGMGIHAWAVEVPTLLING
                     ITEYLYQKFFVFNDAKEKKNR"
     gene            complement(10183..10449)
                     /locus_tag="PLF14_05685"
     CDS             complement(10183..10449)
                     /locus_tag="PLF14_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70821.1"
                     /translation="MNKNPVNKRNVIDYLLIFVYVIMMLISNNQLFRSYSLLGVALLG
                     ICISICIIIFKKDITKQEKTREWIAVSLLVLAAGYSIYRIITLK"
     gene            complement(10446..10907)
                     /locus_tag="PLF14_05690"
     CDS             complement(10446..10907)
                     /locus_tag="PLF14_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HOT70822.1"
                     /translation="MLKKYVVIPALIAALYCALVFIFMPVSFGTVQIRVAEALTVLPY
                     FTPSAIYGLFIGCLLANIIGGAGILDIILGSLSTLVAAFLTYKVKNKWLAPVPPIVIN
                     MFVIGYVLNVYYGMNYWLAVVSVGAGQLVSCMGLGMLLLLQLEKYKERLFE"
     gene            11154..12614
                     /locus_tag="PLF14_05695"
     CDS             11154..12614
                     /locus_tag="PLF14_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70823.1"
                     /translation="MNNLISLFKSIDISGKTVIVLIILIFLAAFVINILIKLKYQKLS
                     KQINNRQNRRAGTFNDEMLNKIVQDYKVAGEINSNNVNTQAIIEKNFEENLKIPTMGE
                     TFVRKSQAMMVTLGLLGTFIGLTISVSELVKVLLQDIGSASLDWNEILVRLAGAAKGM
                     GAAFSTSLVGLFGSVVLNLTLIGIDCEDQKRSLMIDIEEYLDNNIAVLIAKDKETEYT
                     MMNRILKDTFVEFGTKIEDTLRATVESFGDKLTNVVMDVSVSSQALDTTVERFDSAIS
                     TLAVAMRDMSDFNLNLKENVEKMDVSFIKMSESLSDSTNLIIKNYDAIRMFAEDVKNA
                     AGQMAVSNQETLEQLGFLANQVDQTVSALQQLTYTMKQSSEENAASFNNMKDAFEKAI
                     IATSMEISSLTDKIKQSFQQALKESSDIIAEKTADTMEKSMANIYQMSESFENNQKIL
                     AQTIASLPEQTMVYNKSVSGKIQKKLDEIEKAIKND"
     gene            12644..13681
                     /locus_tag="PLF14_05700"
     CDS             12644..13681
                     /locus_tag="PLF14_05700"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOT70824.1"
                     /translation="MKQRVSYLKKVEPENYWPSFADVMSTMVLIMLFLVLIVFIKNII
                     ISINLDEQEATNIATGAQLEATRNDLAAKELALQETQLDLETKQTILIMLEEELAAKE
                     ISLSLLENELAAKQAALELSEEEATNLQNLILILAEQQAALEAQKKELLELIALNTIE
                     LDSMREQLQKVAVLQVDIFEKVKKSIDDALGPGQVTIGDNGNLMITESILFDQGKSDL
                     KPSSLNLIKKLADAFEEVLKDSGENIEAIMIEGHASSEGDFDLNQKLSTDRANAVLLE
                     LFKKNGKLQTQYARYFGAVGYSNSRLLIKNDYLESQKAKNRRIEFSIVVREENIGQII
                     QEYLDSIDKKK"
     gene            13690..14592
                     /locus_tag="PLF14_05705"
     CDS             13690..14592
                     /locus_tag="PLF14_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70825.1"
                     /translation="MARKDFKDLKLYFSNSMISLKEGDYEHAIKGFSNLIDHGIEPQK
                     SVIGLITAYSCLTRYPAALKLYEKNKDIFIDNKPNRNMLVETMTTLLMKETSLLKKNA
                     RGSLSAVFMAKRMKAVHEAYLADKDNLLAIILICYWYAVLGARPYETEQMMKDFLHNE
                     YVDDEFRWKLLEKLAITDKELMDDITIAGMFRRIPRYLDHSYINLLLFSHLCGDDFAS
                     AREKIEVQRMNGVELSDDVMWNYINSSVENNDIDDLSVNFAKRLFAKGWMDPVIGQVF
                     RYAKNNLNIYNVTNETKALDLFGI"
     gene            complement(14649..15200)
                     /locus_tag="PLF14_05710"
     CDS             complement(14649..15200)
                     /locus_tag="PLF14_05710"
                     /inference="COORDINATES: protein motif:HMM:NF013624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HOT70826.1"
                     /translation="MKILISGFKPFANFQDNITRKLVEGLPDVINGNKIYKIILDVTY
                     KTAFDVLDKEINKVRPDIVIATGLAAARKVITTERIALNINHANAADNAGVTKTDSVI
                     IPGAETALTATLEYPECVYKTDIVVKSYSAGTYVCNDVFYRLLHKGTVQAYPYISFIH
                     FPSEENIPYEKQLNTFVDILSCL"
     gene            complement(15197..16057)
                     /locus_tag="PLF14_05715"
     CDS             complement(15197..16057)
                     /locus_tag="PLF14_05715"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT70827.1"
                     /translation="MIIDAHNHADWHKTDLDGFMKNMDDNDIDVTWILSWECPRHEYE
                     NYNGALASYNKKGPVPFEAVLAYKRAYPHRFVLGYCPDPRDPDAIQNLKAATYIHKVQ
                     VCGELKVRMMYDDFDAIRLFRAAGELGLPVIMHFDYEFPRSNISGWPNFWYGGGIDTL
                     ERVLKKCPETIFLGHAPGFWAHISNDDLYDKVAYPKGPVIEGGMIQKLLDKYDNLYCD
                     MSAGSGLNALTRDPEHALRFLNQYQDRILYARDYFDSKHREFLESLDLSQQVKNKLYF
                     ENALKLVPLK"
     gene            complement(16054..17370)
                     /locus_tag="PLF14_05720"
     CDS             complement(16054..17370)
                     /locus_tag="PLF14_05720"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOT70828.1"
                     /translation="MYKTDNNRNKRKNTFKKKGQYKKKRKYDKQTGQPTKFRKRPAEH
                     EKDNQVLNYSGTKFADLHLSEGIMKALNKKGYVYSSTVQSGVIPPLMQYRDVIAKAPT
                     GTGKTFAFGIPLLEQLDNENKDIQALILAPTRELVLQICNELKELSEFMPKVRVEAIY
                     GGKAIEGQIKALKRHPQIIVATPGRLRDHIERKTVDISKVRTAVLDEADRMLDMGFIE
                     EVTDILNMMPDRKNLALLSATMSIGVMDISWLYQRNPVEITVEEHNLDKPDIDQMYVT
                     VQNMRYKIKAVETIMDNNELYKVLIFCNTIRMVRMLTDDLREKGYSANCIYGGKSQAG
                     REKTIRAFKEERLNILVATDVAARGLDITDVDAVINFDLPDDKEQYIHRIGRTGRAMK
                     NGIAVSLITPFDNYELEEIKKYCKSDIKEIKNDGILNLSKEGDAEK"
     gene            complement(17372..17981)
                     /gene="hisIE"
                     /locus_tag="PLF14_05725"
                     /pseudo
     CDS             complement(17372..17981)
                     /gene="hisIE"
                     /locus_tag="PLF14_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514582.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
     gene            complement(17962..18711)
                     /locus_tag="PLF14_05730"
     CDS             complement(17962..18711)
                     /locus_tag="PLF14_05730"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOT70829.1"
                     /translation="MPKAALQLYSIKDIAEQDFYHAMHIAKQVGFDGVEFAGFYGKEA
                     IETKKYLDDLGLLPAGSHSGLNAVVDKFHETMEYNSVIGNKYVAVPYLSEQFHSADGM
                     KTVAEYLNIASENAKKYGIKIGYHNHAFEFTVIDEGKRLFDILAENTVKDIFLQIDTL
                     WAVKGGVDVLEYCKKYKDRLDTLHFKDISEEGKDTELGRGLIDFVSILNVSDVIDWCI
                     LEQEQFDMPKEKSIAISCAYLKGILDAKIAV"
     gene            complement(18760..19476)
                     /locus_tag="PLF14_05735"
     CDS             complement(18760..19476)
                     /locus_tag="PLF14_05735"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOT70830.1"
                     /translation="MDRKKVRNREDLFFIEGRKLLDEALSSRIRITEVYVSDTYYQNN
                     MSYVDKLTVKNLYLVRDGILESLSYMKSPEGILALGIPPKFDVPDVLKKGNPVLVMDR
                     INDPKNAGTMVRSAEAFGMGCVFALEGCADLYNEKSLRASMGSLFRVPYIKVSKEIFT
                     VLKNAGYAIYGLDASGHNIETVSLDKRCALVIGNESDGLDKKIRKYLTETLSIPMQGK
                     TESLNAACAAGIVMYKISLL"
     gene            complement(19531..19875)
                     /gene="rplT"
                     /locus_tag="PLF14_05740"
     CDS             complement(19531..19875)
                     /gene="rplT"
                     /locus_tag="PLF14_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOT70831.1"
                     /translation="MARVKSGVTKRRRHKRVLKLAKGYFGHKSKNFRVAKQQVMKSLN
                     YAYIARRARKRDFRKLWIARINAAARTNGMTYSRFISALKKNGIELNRKVLADMAVND
                     PKAFASLVSSVK"
     gene            complement(19890..20087)
                     /gene="rpmI"
                     /locus_tag="PLF14_05745"
     CDS             complement(19890..20087)
                     /gene="rpmI"
                     /locus_tag="PLF14_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOT70832.1"
                     /translation="MAKLKTHSASKKRFRVTASGKIKMGHAFRRHRLISKTKRAKTDN
                     RATAFASDADAPTIKKLIPYK"
     gene            complement(20100..20630)
                     /gene="infC"
                     /locus_tag="PLF14_05750"
     CDS             complement(20100..20630)
                     /gene="infC"
                     /locus_tag="PLF14_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOT70833.1"
                     /translation="MAAQKGTRSQYRTNRNITAEEVRLIGTDGTAIGIMSVNEALKHS
                     EAAGLDLVEISPNARPPVCRIMDYGKFMFEISKREKEAKKKQKTMELKEIWLKLGIDE
                     HDINFKIKNACKFLADGNKVKVSLSFRGREMTRTQMGAEVLERFTEACSEYGTPEKDP
                     KLEGRHMIVIIAPKKQ"
     gene            complement(20838..21842)
                     /locus_tag="PLF14_05755"
     CDS             complement(20838..21842)
                     /locus_tag="PLF14_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70834.1"
                     /translation="MSKKVLVLGGTGAMGVYLVPLLAKMGYKVDVVALDGGQSENPNI
                     KFMKAKNAKEPAYIGELLKNDYDGIVDFLIYGTKEFKEKYRPLLENTGHYIYLSTYRV
                     YANEEHPIKETSPRLLDVIKEEPFISSEDYSLYKARGEDVLKASGLDNWTTIRPAITY
                     SKRRFQLVTLEADVVIERARHGKTVLLPRQAMDVQATMSWAGDVAKMISRLLFNNAAK
                     RETYTVATAEHNPWRVVADYYKELIGLKYEEVDKEDYISFISPDKNSPYARWQLEYDR
                     MFDRIIDNSKILNIAGMKQSELMPLREGLKRELAVLPADTFTVQSNTSRNMDIFLEKR
                     "
     gene            complement(22023..22826)
                     /locus_tag="PLF14_05760"
     CDS             complement(22023..22826)
                     /locus_tag="PLF14_05760"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HOT70835.1"
                     /translation="MRDKHTHRKFLIPGRPTLCVMIKSRVTSEIIRDIRLALEEGCDA
                     FGLQMESMLPECRTETDFCDIFNAMEDKPAYVTNYRRGNTKSAADDEYLAKELITALE
                     CGGDLIDIRADMFYPTSSEITYNRAAVEKQIRFIEEVHKLGGQVIMSSHVLKFIKTDE
                     VLEIAREQRRRGADIAKIVTAADTESELLENLRISTCLKDKLGIPSLFLCGGSASRKH
                     RLLGPLLGSDIYLVVQESADSPEGQPKLKDAKRLLLMMNYENIPGEKNI"
     gene            complement(22975..23217)
                     /locus_tag="PLF14_05765"
     CDS             complement(22975..23217)
                     /locus_tag="PLF14_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOT70836.1"
                     /translation="MRIDKFLKVSRLIKRRTVANDACEAQKIFANGKAVKPSYQVKKG
                     DILEIKFVNSSVKVRVTDISDNVKKGDASSLYEIVE"
     gene            complement(23204..23998)
                     /gene="mazG"
                     /locus_tag="PLF14_05770"
     CDS             complement(23204..23998)
                     /gene="mazG"
                     /locus_tag="PLF14_05770"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOT70837.1"
                     /translation="MDKHDKKYDFNDLVEIMAKLRSDSGCPWDKEQTHESITTCLIEE
                     AYETCDAIIDNDIEKMTEELGDVLLQVVFHAQIGKENGTFDLSDITDGICRKLIFRHP
                     HIFGDKKMDTAEQVLESWEKLKIKEKGYKSEADSMKNIPKTFPALMRANKIQSKAERV
                     GFDWPDVWGAFDKVYEEAHELKRAIETEDTEKQKEEAGDLLFAVVNVCRFLDIDPEQS
                     LDAASDKFIKRFSHVENSCNDKGQYMREMNLEQLDKYWNEAKNENR"
     gene            complement(24020..24841)
                     /gene="prmC"
                     /locus_tag="PLF14_05775"
     CDS             complement(24020..24841)
                     /gene="prmC"
                     /locus_tag="PLF14_05775"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOT70838.1"
                     /translation="MVISQLIGYCSEIFKDKKEMYFFLEEVFQKDISYFYAHPDEIVD
                     TEKYRLLLEYAERRRKNQPFEYILNKACFYGNNFYVDSHVLIPRNDTEILVEKAIAIG
                     KTMESPRILDLACGSGCAGISVAKELAQSKVIMSDISEKCVEIANKNILINKISNCSA
                     VQSDLFNKIEGQFDIIVSNPPYIPLHEYVALDKQVLEYEPKLALLAGEEGTEYYELIS
                     ADAMKYLKKGGYILYEVGYNQASRVCDILKKYGYTDIKVHKDLHNTDRIVIARSE"
     gene            complement(24835..25779)
                     /locus_tag="PLF14_05780"
     CDS             complement(24835..25779)
                     /locus_tag="PLF14_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HOT70839.1"
                     /translation="MRKTSIGGSALLEGIMMIGPHKKAIAVRKPDGDIVLDVENLPKK
                     WKIAKIPVLRGAYSLIYQMVLSIKALSYAASFYDIEEEDIDKTDEQNEKDKEQPDEKK
                     AETKSSDGELSKTAIFFAVLLSVAITVGLFILLPNFVIGLLKIESAVVNNLLEGVLRI
                     AIFIGYLAAVTLMKDIKRVWMYHGAEHKTINCYEADEELTVENVMKYPTSNRRCGTSF
                     MFLVMAVSIIVFSLVGWHSAILNMVFRILLLPLVAGLAYELVKVAGRYDNVLSKIVSA
                     PGMLFQKLTAKEPDESMVECAIMAFNAVVPEDPEEAKW"
     gene            complement(25829..26029)
                     /gene="rpmE"
                     /locus_tag="PLF14_05785"
     CDS             complement(25829..26029)
                     /gene="rpmE"
                     /locus_tag="PLF14_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005827805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOT70840.1"
                     /translation="MKKDIHPKYYKTTATCACGNEFEIGSTRKDIKVEICSNCHPYFT
                     GKQKFVDTGGRVDKFKKKYGIS"
     gene            26266..27534
                     /locus_tag="PLF14_05790"
     CDS             26266..27534
                     /locus_tag="PLF14_05790"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HOT70841.1"
                     /translation="MRKIKCSNKHLTLDERKIIQTGIENGSTKADIARVINKDSSTVA
                     KEIRKHRIKKPRNTYNRPTICIHIRECGKRCIGNCERYEEPKCRRRDVSPGACNKCDK
                     YSKCHLDRYIYNAQRADEAYRQTLIESREGIGISSKEMLRIGNIIAPLLKKGQSVHQI
                     LAAHRNEIRLSERTLYHYIETGVFETFGIDCFSLKEQVNRKRFKGKYKPRKEKADYTG
                     RTYNDFLNLLSEDAEISYVEMDTVMNSLSGPYIQTIMHKRTKLMIGFIHREKTSKSMS
                     DTFDMLQQKLGRNLFTALFPVVLADRGAEFEKYEMFESDSDGLTRCRMFYCDPMQSSQ
                     KPHVENNHNYVRDIIPNGYPLDKIVQEDLDIMFSHINSTPRKSLADKTPYEMFVFLYG
                     KETADLLNMSCIERDEVTLKPTLLNHVYKR"
     gene            complement(27842..29401)
                     /gene="rho"
                     /locus_tag="PLF14_05795"
     CDS             complement(27842..29401)
                     /gene="rho"
                     /locus_tag="PLF14_05795"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOT70842.1"
                     /translation="MNEVEILKSKSIADLRVIAKTLGVKSVTVMKKAELIERILEITK
                     QEQPDIEKEQPKEEVAVSEAAKILEAVTQETPKKPRYLLSKMAKESEVAKPVKEERKT
                     RHVIKSHDSATIVPPGQAQSTSVQETADSQTDSDLSEECEDGQYDQVCGVLEVLPDGY
                     GFLRTDNYSYGPKDIYVAPNQIRKYGVKTGDMIFGTGKPKKDNEKFQALQNVITINDE
                     DPSKVFTRKSFDTLTPIFPEERLTLENNPKDYSTRIIDLIAPIGKGQRGMIVSPPKAG
                     KTVLLKKIANSITLNHPNVELIVLLIDERPEEVTDMQRSVKGEIVYSTFDKVPENHVK
                     VSEMVLERAQRMVEHGKDVVILLDSITRLARAHNLTMTPTGRTLSGGLDPGALHAPKK
                     FFGSARNIEFGGSLTVIATALVETGSRMDDVIYEEFKGTGNMELHLDRKLAERRIFPA
                     IDINKSGTRREELLLGKKELESIWTIRRAMSNMGTAEVTEWMIDKLMKTRNNAEFVSS
                     INIKQTAVMDY"
     gene            complement(29582..30370)
                     /locus_tag="PLF14_05800"
     CDS             complement(29582..30370)
                     /locus_tag="PLF14_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014015032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HOT70843.1"
                     /translation="MDDTIKNKDGFIIDMDGVLYHGNNLLDGALEFIDWLQNNNKKYL
                     FLTNSSERSPRELSQKLARMGIVVGDEHFYTSAIATALFLASQSPRCTAYVIGEPGLI
                     NALYEVGITMNDVSPKYVIIGETRSYSFEKIEKAVQLVQRGAKLIGTNPDRTGPTEKG
                     IMPATGALAVPIEIATGKKAYYIGKPNPLMMRHALKKLGTDVKNTIIIGDRMDTDIVA
                     GIESEIDTILVLSGVTNHQNIQNFSYRPKYIYDNIGEVIKKSKI"
     gene            complement(30373..31665)
                     /locus_tag="PLF14_05805"
     CDS             complement(30373..31665)
                     /locus_tag="PLF14_05805"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOT70844.1"
                     /translation="MKYQLVLAGGGARGSYQIGVWKALRELNIEVDMVIGTSVGSING
                     AAFVCGAFEQAEYLWKTLDMPTLFDITYDKEDGEPGSVEYIESILKNKGLDTSTLKGY
                     LEQVIDEEVLRNSPVDYALVTVSLTKMKPVILFKKDIPEGKVVDFILASSAVPGFKKQ
                     EIDGELYLDGGFYDNLPINVLIEHGYKNIIAVSNAAIGVYRRVQQSDAKILYIENQQI
                     EGGILEFDPELAARNMLLGYLDGMKAFGKFIGKNYYISDNATHDYAKPLTDREIEMLY
                     LNVKKSKRFVTESVLQFSLLRTLRNNTDGVLTAKRMFFAGMEITANMFGIEKIRAYTP
                     EQLITKIMAAYRSAKSAHRQIMKEKNNFFKNLIDKIWDKNRIRFNGKAAKKLYLGQVL
                     IDKDNPSINLITTTEHLIANLFIYLILWRRKKIKKDRR"
     gene            complement(31722..32492)
                     /locus_tag="PLF14_05810"
     CDS             complement(31722..32492)
                     /locus_tag="PLF14_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70845.1"
                     /translation="MLKRKTHTEGRITAESCICCFVLIFVLLLSIQLNGYIKAHSDLL
                     SELDRRLVNGAVAYHASGIYLVDVITQPEETDINNAIFFAVPYDDFFTLSVFADYSGI
                     LKKNRVYVRSVSSKWAGDGKGVVKENIWELDPLERGQVIHKMMGANLDHNFPTLDIYD
                     GYTKEAVSIVSINTQEDSYKSGTELKRKIKKHIDSMDKFTYGEYKGYSVSGEDIREKT
                     VLVVIPNAKLTGHQTKQINDMFKYAKKAGINLEIKKFQ"
     gene            complement(32467..34449)
                     /locus_tag="PLF14_05815"
     CDS             complement(32467..34449)
                     /locus_tag="PLF14_05815"
                     /inference="COORDINATES: protein motif:HMM:NF039199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5702 domain-containing protein"
                     /protein_id="HOT70846.1"
                     /translation="MKPSSNRKKGVITAFVAVITPVLILLVLVLSDIYLAKNALNAAK
                     NAAKASLGCVLSQYSTYMKERYSLYGYEMSDREAALRITEALNNSSISRGLFEMEIVN
                     VEVERNKPLTSYPVISAQINRLMSDEIFRTVLAETNERIEMFSKIKDVIRTLNVKMQI
                     DELIGGLKNAHTLLKETVEEINSSQYYHDLMEIIRYADSLYGEMLSYMKSGDKEQTDT
                     AQVLKKQVTDILKTNVDNIIYVLKEYNKKAVNLLNEMINTYADIHILSENLKGIVDGI
                     EDCPEQLKAVLKICADTVYKMETSLSVSVLQNIKAVLDRNVDCLEQTMKHMLDTVSEE
                     KDVSLYDVFCDGFEMYYDAVYNGDVLDGFFASVIDDIVKDQRSLLKKMADEVLESIII
                     EDIRIDDFINLVSEGYEDTYIFQADATGNTSKSISESFTDLSEMLSQIKEDIMINEYI
                     MLYMNGFTDSDTDQGENRYLDGEIEYIIFGNRDNRKNVQSCKASLMALRFACNAIHVY
                     TDLPKTTKADALAACLAGSWTFGAAAPIAKNLILCSWAIAESAYDTEALCRGAKCPVI
                     KMDGDWNLDIGLEKGLSKTPDWLKTDYDDYLRLLLCTKSNNVKVLRLLDLIALNSPDN
                     MDMSSVCCGIKVGVTLKFKGLLGTERSFYVEAEDSY"
     gene            complement(34430..34675)
                     /locus_tag="PLF14_05820"
     CDS             complement(34430..34675)
                     /locus_tag="PLF14_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HOT70847.1"
                     /translation="MKYYHRTMGYLYGKKERKGSAAAEAAIVLPLILMLVISVMMMIV
                     KISCEEIEYTEYDLTSEIRTYDGIKRKGDIIFETFFK"
     gene            34904..35638
                     /locus_tag="PLF14_05825"
     CDS             34904..35638
                     /locus_tag="PLF14_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HOT70848.1"
                     /translation="MTFDPNSKLMYIMVAIVLLYVTAQAVFFLVKAIKRAKELNISSS
                     TVKKTILSSAIFTIAPAISILMGVLTMAGFLGLPFPWLRLSILGALTYEVTAATSAAK
                     SLGITDLSTLVTDPKVFATMAWVMHLGIFTGLILVPLFLKKIQRGLIKIKERDEKWSD
                     AFLTALFLGMISAFLGLIFADIKSGLIGWIPVFVMIVSALIMSVLGILVKKFKIKWLE
                     DFALPITMLVAMALSIPITNLIESLI"
     gene            35648..36322
                     /locus_tag="PLF14_05830"
     CDS             35648..36322
                     /locus_tag="PLF14_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009267354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70849.1"
                     /translation="MKKNKTTKSYDDQIHFHGRIWMLGALALFTLFPTAICVIYNAWP
                     SAGPLFKALAGVAPIFWTVGVIEILTYTPMLGSGGSYLSFVTGNISNLKAPCALNAMN
                     ASKVKPGTDEGEVISTIAIAVSSIVTIVIIAIGVLGLTAIRPLLESEALKPAFANILP
                     ALFGGLGVVYISKNWKIGLAPLLFMLALFIISPIDLSGYVGVLVPVGAIIAIIAARIL
                     YKKDKL"
     gene            36333..37766
                     /locus_tag="PLF14_05835"
     CDS             36333..37766
                     /locus_tag="PLF14_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOT70850.1"
                     /translation="MWWLIFPALFIIFLLILIIRALLFKPYPITKPEITDIELDNEGI
                     INRLSELIKCKTVSNMDFEKVDFAEFEKLHKLIEKFYPDVFRKCEFEKVSNTGLLFRW
                     PGKESDAPTVLMAHYDVVPANEDQWDKPAFEGIVENNEVWGRGTLDTKGTFTSILEAT
                     NLLIKQGFVPQNDIYLSFSGDEEVSGPSCPAIVELFKKRNIVPALVIDEGGAVVEDVF
                     PGVKARCALVGTAEKGAMDVRMTMKSQGGHASSPKPHTIIGQLSQAVVNIENNPFKFQ
                     LTPPVKKMFDNLGRHSSFVFKVIFANLWCFSPVLDLMCKKSGGELNALMRTTVAFTMA
                     SGSSAANVLPPKASFTANLRLIGTDTCQSAIEYLKKTVKNDNIEFEMIHGSDPSIYSS
                     TDCKGWDILTDAIKQTWSDAIVSPYLMFAASDSRHFSKISDKVYRFSAMYLTKEQRAL
                     IHGNNERIPCDTVYDMVKFYVRVIKQS"
     gene            37929..39074
                     /locus_tag="PLF14_05840"
     CDS             37929..39074
                     /locus_tag="PLF14_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOT70851.1"
                     /translation="MINRSGEYVSNLSGKLEYKSFRPAKLPPIPPIQPDEEMLALLLE
                     ANKKVSLLDGLAARIPDMDLFVAMYVRKEALVSSQIEGTQCTLEDILDPNLDTNTNAD
                     VSEVVNYIKAMEFAVEQLNTLPLCNRLIKETHAVLLQGVRGAEKTPGEFRTSQNWLGS
                     PGSTLKNARYIPPNVQDMNIAMSDLEKYINSEDSLDTLIRAALIHYQFETIHPFLDGN
                     GRIGRLLIALFLMQTQTLRSPVLYMSCYLKANRIEYYDRLSEVRKSGNYEQWVTFFLR
                     AIVETATDAIETIDKLTEVKNISIQKLDDITPSIKARLETLLTYLEKNPIIDTKKTAE
                     ALGFSYNSTAKYVDILCKKGILEHSRKAGKTKIYSYTDYLNILRKGT"
     gene            complement(39100..39900)
                     /locus_tag="PLF14_05845"
     CDS             complement(39100..39900)
                     /locus_tag="PLF14_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70852.1"
                     /translation="MQIELYKKLYGSLVNYAAAMGIDEVSLQRYFYPKRDITSKGILI
                     RLCSSLQNSGRMRNSIKFNDKDTTNRMIIKKVLFDFNTKKSASIYHEWTSIYDAMLDM
                     GIIDKGMKKHTATNWEKYCRGLYDGLQFLTVDNGEKIIADLVATSNMTDKELKELHAI
                     SKKIHGLGLSLTCDWLKDCGCTWLAKPDVHIIKVVRYMQSDDKLNEEDVIKEMFSWAQ
                     AVKKSGTDKNATAYKIDKIIWLLCTGTFYLDDNRIGREAIYRTIDSLR"
     gene            40098..41480
                     /gene="cysS"
                     /locus_tag="PLF14_05850"
     CDS             40098..41480
                     /gene="cysS"
                     /locus_tag="PLF14_05850"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOT70853.1"
                     /translation="MKVYNTLTKKKEQLVPIDDKTVRMYSCGPTVYSYAHIGNMKAFL
                     FMDFLKRSLNYLGYKVKNVMNITDVGHLVSDADEGEDKMIKTAKSTNKTPWEIAAMYT
                     DAFLADIKKLNIQRPNVLCKASEHIEEMLEMVYKLYDEGYAYETSDGIYFDIAKFPQY
                     GRLSNLDLEGQLAGARVAVNEEKRHPADFALWKKAPKEHIMQWDSRWGMGYPGWHIEC
                     SAMSRKYLGDQFDIHTGGVDHIPIHHENEIAQSDAFLKKQAVKYWMHCEFLQVDGGKM
                     SKSMGTAYLLSDLEEKGYEPLAFRYMCLNAHYGKKLNFTWDGIEAAQKSLNRLSNLVL
                     EHKESDTHIDSNVIEEYKQQFREALQDDLNLPLAMSTVWNAARNNVKSKSIYNLLMDF
                     DKVLALDLDKKRTEAADDIPEEVTLLAEQRLEARKNKDWKKSDQLRDRIRQLGYSIND
                     TPAGQVIKKG"
     gene            41482..>41816
                     /locus_tag="PLF14_05855"
     CDS             41482..>41816
                     /locus_tag="PLF14_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006442992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HOT70854.1"
                     /translation="MLIKVFQKGFNFSQDGPGNRLVYHLQGCNMNCPWCSNPEGIEKD
                     GSIMVTEGVDDAVCPNGAISSGMLDSKLCRGCPKMCLQTLHMGIKLSCDTYHTDEIVS
                     EAARSKSMF"
BASE COUNT        11751 a         9370 c         7556 g        13139 t
ORIGIN      
        1 acggcttcat ttattgccac ggatttgggt acatcctcaa gattcaatat ctcataatat
       61 gcaagtctca tgattgccag ttctatcttg ggaattcttt caatacgcca gttcttggca
      121 agatacatgg atagcaccga gtcgatgtct ttgcagttct cattgatata tgttaccgcc
      181 ttttttatat attcctttgc ggtattcttt acatcattat caaggaaata ctgttccatc
      241 tgctcattta tatcatcgtc atttatctgt atctggtata caagccgaag agcatgttcc
      301 cttgtttttc ttcttcccat aacggttcct ttaataatta atttattccg tccacaaaaa
      361 ctcttatgct atgcaattct atgccggtac tttttaacac ttcctcttta acaatgctct
      421 gaatcttgga agatatctcc ggtatgagaa ccgatgactt gactttcacc gatatatcca
      481 tacctacctg cgaattgctg aaatccacct tggttctcac atcggtcacc gaatcaaacc
      541 gacttactat actcttgacc atagtttcca gcgaataggc cgtaatactg atctgtcctg
      601 tcggggtggt cagattgacc gtttctacgg gtttttcttc tttgcgtaaa agagacacta
      661 taaagaaagc ttcaaatacc agaatcagca acgcaaccag gaatataacc cctctttgta
      721 ccgagaaaaa ccttgcatcg acaaaatcct cgaaagctct ctcgtaatcc atcggatcga
      781 gacatgcatg tatcgtaacg gcacataaga caacaatcaa tattatattt acagtataaa
      841 aaaacttcct gcccttttta aaaaatttca tttctgctcc tccccgggtt tatgtatatt
      901 tacaccctga acatggatat taacttccgt aactttacta ccggtcattt tttccaccag
      961 tttttttacc ttttcctgta tactccatgc cacttcgggt attcttactc ccaattccac
     1021 aataatatat atattgacat gaatgccttc ctcatcggtc tcaatcttga cgcctttgga
     1081 aagattctta atccccaatg cctgtgcaag ttcgccggta atccctccgc tcattgcata
     1141 cacgccgtct tcttccattg cggcaattcc ggccaatatc gctattacct cgttagtcac
     1201 cttaagttgc cctaaggatg tttccagtcc gttgattttc agaacatcat cagccataac
     1261 atacctcata ataattatac attttgcacg atatatttac aacataaccg aaagagactt
     1321 acgtcaagaa tcctaaatcc cgcaaaattt tagagtgaac caaaaattaa tttaaaagtt
     1381 aaaaaacata gaacatccgt taaagtgccc gatttattct gtacaccagt aaatcaaagg
     1441 acatatcctc tacatcgtag gtgttttctc tttgaaagcc cagttttgca tatagtttag
     1501 aggcaacaac attatcggta tccgataaaa ccgacaactc cttataccct ttttctttca
     1561 actcctcaat tagatgcatt atcaaagcgg ttgcaatctt tttgtttcga tagtcttctt
     1621 tgaccaataa tcccgacata tatgccctta tattttcttc agccgcagcc ttgtcacttg
     1681 aaacatatgt caatgtgaca tcggcaatac attcatcatt tacaaaaacc gcatagcaat
     1741 cgtctttttt atcaacaacc cgattatagc gctcaataaa atcctcatca tcttttgcat
     1801 atcctcgaag ttcggcaatg cacataaatg attcggcatc aagttttctt atcgtataat
     1861 ccattttaaa taataatcaa ctctttattc gagtgattaa gagtatagca gccgtcttct
     1921 tgcacgacaa cggtattttc tatccggact ccgcccgcat cgggaatata tattcccggc
     1981 tctacggtaa cggtcatatt acttttcaga acggttgtgc tttgggtgga aagcctgggt
     2041 tcttcatgta ccagcaaacc cactccgtgt cccgtactgt gcccgaaata ctttccgtac
     2101 ccttcattac ctattatatc tcttgcaatc ttatcaacat ccgctcccgt tacaccttct
     2161 ctgacataat cctgagcact cttttgtgcg tcatacacta tgttatatat cttcttcatt
     2221 ttaggtgacg gagaacccag gaaaaaagtc ctggtcatat cggaacaata ccctttgtag
     2281 tttgctccga aatccaaagt aaccggatca tgattcataa gtttcttatg gtcgggaaca
     2341 ccgtgaggca tactggtctt tttacccgat atcgctatgg tttcaaaagc cggaaaggat
     2401 gctccgtttt tcttcatcac atactccagt tcggcagcta cctctatttc tgtcattccc
     2461 gcttttacag tattcagtat atgtgtaaaa gcgatgtccg ctatggaagc ggcttttgcc
     2521 atcagagaca attccttttg gtctttaacc gagcggacgg cgagtaaccg gtcggaaaca
     2581 tcaatatatt cacaatcctt cagacctttt ttcaaagcct caaagtagcc gtatgaaacg
     2641 gttttttcaa atcctatgcg caattcactt ttagtgaaaa gtttggataa taaggagagt
     2701 acgcttttca tgccttcctg aatcaacacc acttcgtaac cgtaagcgga tatcagttcg
     2761 gccgcttcaa tgtaacgaaa atccgttgca aagtatgctt tatcacgtgt aaatatcaat
     2821 atactgctgg atccggaaaa agaagaaaga taaagcgtat tatctctgga cgttatgaca
     2881 gctccgtcca agttttcttc ctccattatt tttctcatac aatcaactct tttactgaac
     2941 atacggcacc tcataaatcc ttcttcatat attgctcata cataatttta ctcttcaacg
     3001 catcggttgc catggtatta tccgattcgc atattataac aggtttcata tcatacttat
     3061 acagtaccgg tataaggtcc tggaaatagg gaccgtactt gtcatcttca aatgtaaggt
     3121 gctttttttc gccgccttgc gtatattcaa tctgggaaaa atgcacatgc attattcttg
     3181 tccgctccaa tcctattccg tttataagtt tcaacaccac tctttcaaaa tcttccggtg
     3241 ttttcagagt accgtgagtt cttgcattta aatgaccgaa atctatggta ggcagtatcc
     3301 tttcgtcatt ggcgcaaagg taaataacct cttccaaatt gcccagctga tttactttac
     3361 ccatcaactc cggacagaat attaccgatt ggtcatcaat atcatccaaa aagtttttaa
     3421 gaaatatatg tgccctcttt atggcattct ccctggtatc tcccatggga gagccggtat
     3481 gaacgcatac tcttgtagca cccatctgtt ttgcagcgat atatgatttg tgcatatgca
     3541 taagggaact ttgcacttta tccggctcct ttgtcgccat gtttataaag taaggggcgt
     3601 gaagagataa agctatatcg tgtttttttg catgctcacc tatctttaat gcggcttgct
     3661 cccctatatt tactcccctg ccgctttgat actcatacgc attcaaaccc atatcctgca
     3721 accacttcat ggcttcaaag gtattcttat atccctgttc gtaaaaagaa ggctcattgc
     3781 ctgccggtcc gaatcttatc atatcttttc tatcccttta atatattttc tcattatcct
     3841 gtcctcaaaa atcctcttga accggacata cagcgattca tgattgttca ttctcttagt
     3901 cataacggtc agaaatcccg cttttttgcc tcctcttatg tcggtaaaaa gctgatctcc
     3961 tatcattgcg catttatgcg gctcaaggtc taatatgcgt aacacaataa gaaaaccctg
     4021 cttaagcggc ttaaatgccc tgtacacata ttctaacccg aaaccggtgg caatcaaagc
     4081 ggttcttttt tttgaagcat tggataaaat acatatggaa aagcccatat ttttcaaatt
     4141 gtttattaaa gtaagtgctt ttttgtgaat ttcctcgtca tacatcccta ccaatgtgtt
     4201 atctatatca aaacataagc ctcttattcc catatcatac agtcttttat aatctatatc
     4261 cgatattcct tgtgctatgt aatccggtaa atatttatac attaaatttc ctcaaaaaaa
     4321 cctgtaatca tttgtaatta ctccgatatt ttagtataat atactgaaaa tgaaattaag
     4381 gcaaggacca aagaacatga atatttccat taacttaacc aagaaaccca aaacaaaacc
     4441 cgacgaaagc aaactgtctt tcggtaaaca gttcaccgat catatgtttc tgatgaacta
     4501 tactccggaa aaaggctggc acaatgcaag aatagagccg tacggacctc tgtccttaga
     4561 tccgtccacc aatgtgttac attacggtca gggcgttttt gaaggactga aagcttacag
     4621 gacaaaagat aacaaggtgt tgttattccg tcccgacgag aatgccaaga gactgagagc
     4681 atcctgcgag cgactgtgta taccgcctat tgatgaagcg gatgtggtac aggcgatagt
     4741 aacgcttgta aaaaccgata tcgactgggt accttccgca ccgggaactt ctttgtacat
     4801 ccgtccgttt ataatctcgg tagatccttt cttaggagtg catccttcct atacatatct
     4861 ttttatgata ataatcggtc cggtaggtcc gtattatccc ggaggactca atcccaccag
     4921 tatttatgtg gaagacttct ataaacgtgc ctccaaaggc ggaatgggac atgccaaaac
     4981 catgggtaat tacgcggcaa gccttatcgc acaggaaaag gccataaaac tcaattgtat
     5041 tcagactttg tggctggatg cgcatgaagg taagtatatt gaagaagtag gcgcaatgaa
     5101 cgtattcttt gttatacgtg ataagattgt tacaccggaa ctttcgggaa gcatattacc
     5161 cggaataacc agaaaaagca cattggagat tgcagaagca ttgggctaca ggacggagga
     5221 aagaaaaata tccgttgatg aggttataaa agcgctgaaa aacggtgaca tgaaagaatg
     5281 tttcggaacg ggaacggcag cggtaatatc ccccgtagga gaacttgtat acaaggatgt
     5341 gcgttacgtt attaatgaca ataagatagg taagatatcc caaaagctct atgatacgct
     5401 tacgggcata cagtacggta tcattaagga tacaaggggt tggactttag aagttaagta
     5461 ataaactaaa caaacctttt aaggcttcta attatttacc acaaccgcat gctttgcaac
     5521 atattccatt accttattca gcagtatgct ggaggtaatg gaatttttat cgtaatcttt
     5581 aaacaaatcc tgagtgttgt catatccgta atactcggcg taattggcat agcctttatc
     5641 caaatcgctt ttggttaact ttattttctc caaccccgca atggcttgcg atatcatttc
     5701 ctgcttcact ccgtcacggg cgctttccag attaatccgg ttaaactctt cttcggtctg
     5761 ccccaatgcg tcaatacaga aatcgttgaa ggagctgtac ccgtaatata tcgtgtagta
     5821 ttcataatat tctcttatct catccactct cttttgaacg gtatcgggat attttattac
     5881 tactgtatta ttcaccacgg ttttccatgc ttcttgagtt tttttatact tgaatatctc
     5941 ttttttcaga tattcgttgt attcttccac cgtcttatac tcactatcca ggctttttat
     6001 aaactcatcg gtatactgag gcggtactcg ccttgtaact ttattcacta caacattgaa
     6061 aactacctct ttgcctgcaa gtgcggacac actattgtct ttatctttgt aatttacggg
     6121 aaatgtaaga ttcaacacaa ccgtctgtcc gataactgcc cctatcagtc cttcttcaaa
     6181 tccctctata aaagtgtgtg ttcctatggc caaatcataa cctgtttcgg caccgcccgc
     6241 aaaaggtctt ccgtctacca gaccggaata atcaatgtta gcaatatcgc ctgatttaac
     6301 ctctcctcta tccagaacaa cggtttttgc atacttgtcc aaaagaatat ctgtctcttt
     6361 ttgcaaatct tcttcggtta cggcaatcac gttatagtcc aactgaagat tcatatattc
     6421 ggcaagctct atatacctgc tgtacttata aggagcgcat ccggtaagca gtattgtaag
     6481 taacagcatc gttaccgtga taactgaaaa tatccttatt tctttagtgt aattaatctt
     6541 acttttcttc atatctgtta tgtcccttaa tttactctgt ctcgttatta tcatcctctt
     6601 gatcaaagca gtaaaaatct atggcatccc atatctcttc catacctctt tttgccgtaa
     6661 cggaagtcag tatgaacggc gtatcggggt caatatccag cattcccgct atgcttcttg
     6721 ctctattgat tgcttgagat ttggagagtt tatcgcactt atttacagct ataacaaacg
     6781 gcaaacctat agtattcaaa aactctatca tatgcaaatc atcttccgtg gggtcatggc
     6841 gaatgtccaa tatctgaatt atgagccgta tattctttct ttgagttaaa taccgttcgg
     6901 caagtttgcc gtacaacttt gtttcctgct ttgaagcttt ggagtatccg taaccgggaa
     6961 ggtccaccat tctcatgaca ccgtttattt catagaagtt aacaagacgg gttttaccgg
     7021 gagccgatgc cgagtaggca agattctttc tgttgcaaag agcgtttata aggccggatt
     7081 tacccacatt ggattttccg gaaaacacaa cttccggcaa ctcatcatca ggccattgct
     7141 ccgcttttgc cgcacttatt ataaacttgg aatcttttac taacaaaccg gttctccttt
     7201 gccaatatta tacattacat tcccaatatt gcgaactttg tattatttcc ataaaaaaag
     7261 cggtttcccg cttttttatg tttaatttcc gacttatctt aatactctcc cgccgtattg
     7321 tcacatccca atacatgaac aagcttatgc ttaaccaact gcttgatatt ttctcttgca
     7381 ggtttcagat actgtctcgg atcgaaatga tcgggatgca atgcaaaata ctctcttatg
     7441 gatgaagtca ttgcgagcct cagatcggag tctatattta tcttacatac cgccatacct
     7501 gcagcctttc tcagcatttc ttcgggaact ccctgggcac cgggcatttt tccgccgtat
     7561 ttgtttatct tttccacaaa ctccggaata accgatgatg caccgtgaag aactatcggg
     7621 aaaccgggca atcttctctg cacttcttcc agtatgtcaa atctcagttt ggcttcaccc
     7681 tggaacttat atgctccgtg gcttgtacct atcgctattg ccagtgagtc cactcccgtc
     7741 tttgatacaa actcttccac ttcatcggga tcggtatacg aggaatcttc ttttttgact
     7801 ttaatttcat cctccacccc cgccagtctg cccaattctc cttcgaccac tacacctttt
     7861 gcatgtgcat attccacaac ctgcctggtt actttaatat tctcttcaag tggaagatga
     7921 gaaccgtcta tcattaccga agtaaaaccg ccgtctatac aggatttaca tgtttcaaaa
     7981 ctgtcgccgt ggtccaggtg aacacatatg ggaagcccgg tatcttcaag agccgcttca
     8041 attaatttca taagataaac atgctttgca tatcttctgg ctccggcaga aacctgaaga
     8101 atcaggggtg cctgtacttc cattgcggct tccgttatac cttggataat ctccatattg
     8161 tttacattga atgcgcctat cgcatatccg cctttatagg ctttttcgaa cattttcttt
     8221 gatgttataa gtgccatatc tatatcctct ctactttctt gctgatactt ttttcagttt
     8281 tgcaaattta ggcagtcctt cttcaaaagg aagaccggaa tctccctgta tcaaaggaag
     8341 tgcataatcg atgaaatctt tctttaagaa attcttctct tggttaaacc actccaaagg
     8401 aacggttttc tcggcattgg ctactttagc aagaggtata agaactgttt tgataccgta
     8461 tttacctttt tcgtttgttc tttcaaaacc tatcatgtaa tcggtatgtc cctctaccgc
     8521 agcttttacc gcctgctgcc ctgcaagaaa cgcctcttca atatcggttc ttgagcctat
     8581 atgtgccgca caacgctgca taaggctgaa ctctatggaa cgaactttac atttgaattg
     8641 ttcctcaact atttcaccta agattgctgc cgctccgccc aactgagaat gaccgaaagc
     8701 atctttttta agcgattgac ccatttccgc aatatatctg ccctctttgt ccttgcatcc
     8761 ttcggaaacc gcaacaataa ctttatggtc gggtttactg tcatatactt tctttacatc
     8821 ggaaaggaat ttctcaatgg tgaaaggcac ttcgggaagg tatataaggt cgggaccggc
     8881 tcctttgtat acagccaaca aacttgccgc cgtaagccat cccgcatttc ttcccattat
     8941 ctctattacc gtaatcatag gggtatcata cacagaggca tcatgataaa cctccatgat
     9001 ggttgttgcc acatatttgg ctgccgaacc gtatccgggg caatggtcgg ttattgcaag
     9061 gtcattgtct atggtcttgg gaacacctat aactctgcat tcataccctg catcggctaa
     9121 gtatttactt actttattgc aagtatccat agagtcgttt ccgccgttat agaagaaata
     9181 ccttatgtta tacttcctga aaacctctaa aagtctttta tagtcggtat catcaacatc
     9241 cgcattcttc agcttatacc ttaccgaacc caaagcggat gaaggtgtag tcttcaatag
     9301 ttccaactgt tcttttgatt cttttgatat gtcataaaat tcttcgttca gaattccttt
     9361 gattccgtgt gcggctccgt aaactgcagt gatacattgc tctttaagtg cctgctgaaa
     9421 aactcccgat gcactggaat ttataaccgc tgtcggtccg cccgattgtc cgaaaatcgc
     9481 agctcctttt aacattgtca ttcatatacc tccatgactt aaatatccat ataataatat
     9541 cataacaggc atcataatac aataatgagc ttttgccgtt tatcggtttt tcttctcttt
     9601 tgcatcattg aaaacaaaga acttctgata caggtattcc gttataccgt ttataagcaa
     9661 tgtcggaact tcaacagccc atgcatgaat gcccattcct tcaactcccc agattcctgt
     9721 cgttaaggct cctgtccaga acatggacag cggcgtaaac acaacataat acagtgccac
     9781 tttaagcatg gctttaggta cattgactgc cgatttaaag gtgtatttcc tgttgagtgt
     9841 aaagttccac agtaccgata aaatcaaaga tacaaggtga gcaagccacg gtacccactt
     9901 cattgcatcg tgaaacagtg catatgtccc gacctgtatg gctccggcgg aacctgccac
     9961 aatgaaatat tttattgcct gcagagtttc tcttttagcc tttattctct taatcttctc
    10021 aggatcggtt tcggcatctt caagtgaccg ttttttctcg gaacgaattt tcattttatg
    10081 aaaaagtgta agcaccttat ctgccgcttt gctctcatgt tccatatatc cctcccaagc
    10141 agtacttgaa ttactgcttt tttcagccat aagctaaaga ctttacttaa gcgtaataat
    10201 cctgtatatc gaataacccg cagccaacac aagcaggctt acggctatcc attcccttgt
    10261 cttttcctgc ttggtaatgt cttttttaaa tataattata cagatggaaa tacatatacc
    10321 gagaagtgcc acgcctaaaa gcgaataact gcgaaagagc tgattgttgg aaatcagcat
    10381 catgataaca tagacgaata tgaggagata atctatcaca tttcttttat taacgggatt
    10441 tttattcatt caaataacct ctctttgtat ttctccagct gtaacagaag caacataccc
    10501 aatcccatac aggataccag ctgccccgct ccgaccgaca caaccgcaag ccagtaattc
    10561 attccgtaat atacattgag gacatatcct atcacaaaca tatttattac tatcgggggc
    10621 accggtgcca gccatttgtt ctttactttg tatgttaaaa atgccgccac aagtgtagac
    10681 agcgaaccca gtataatatc cagtattccg gcccctccga ttatgtttgc aagtaaacag
    10741 ccgataaaaa gaccgtatat tgcagacggt gtaaaatacg gcagaacggt cagcgcttcc
    10801 gccactctta tctgcacagt tccgaaactc acaggcataa atatgaatac aagcgcgcag
    10861 tacagagcag ctattaatgc cggtataaca acatactttt tcagcatgca gcctccctct
    10921 tttcttggaa aagtatacca cagcgctata taattgtgaa ctttttgtta catcaaaaca
    10981 tctaattgaa gaacaataac agtgttttaa gttaaaaaac ctgttaattg tgacaaaaac
    11041 aggtgtttta tgttatcagg acgggaataa tgtaaacatt ctgtgaataa tgaaaaaaac
    11101 gcgttttttt atggtagaat tactgtaata aaaccaaatt ctaaggagat catatgaata
    11161 acctaatatc attatttaaa tcgatagaca tatcaggaaa aaccgtaata gtacttatca
    11221 tacttatttt cctggccgca tttgttatca acatccttat taagttaaag tatcagaaac
    11281 tcagtaagca gattaataac aggcagaaca gacgggcagg tacatttaac gatgagatgc
    11341 tcaacaaaat tgttcaggac tacaaagttg caggtgagat aaacagcaac aatgtcaaca
    11401 ctcaagccat tattgaaaag aatttcgagg agaatctgaa aattcctaca atgggcgaaa
    11461 cattcgtaag aaagagccag gccatgatgg ttacattggg actgttggga acattcatag
    11521 gactgacaat ttcggtaagc gaactggtta aggttctgct tcaggatata gggtcggcat
    11581 cattggactg gaacgagata cttgtaagac ttgccggtgc cgcaaaagga atgggagccg
    11641 ctttctccac cagccttgta ggacttttcg gttcggttgt tctcaactta accttaatcg
    11701 gcattgactg cgaagaccag aaaaggtcac tgatgattga tatagaagag tatcttgaca
    11761 ataatatcgc agtactgata gccaaagaca aggaaaccga gtataccatg atgaacagaa
    11821 tactcaagga taccttcgtg gaattcggta caaagataga agacactctc agagcaacag
    11881 tggaatcctt cggtgacaaa ctgaccaatg tggtcatgga tgtttcggtt tcgtcacagg
    11941 cacttgacac tactgtggaa agattcgaca gcgcaatatc cacattggcc gttgccatga
    12001 gagacatgtc ggacttcaac cttaacctta aagaaaacgt agaaaagatg gatgtatctt
    12061 tcattaagat gagcgaatcg ttatccgatt ccaccaacct cataatcaag aactacgatg
    12121 ctataaggat gtttgccgaa gatgttaaga atgctgcagg gcagatggca gtttccaatc
    12181 aggaaaccct tgaacaactc ggcttcctgg caaatcaggt agatcagacc gtttccgctt
    12241 tgcaacagct gacatataca atgaagcaat ccagtgagga aaatgccgca tcattcaaca
    12301 atatgaaaga tgcatttgaa aaagccataa tcgctacatc catggaaata tcttcactga
    12361 ccgataagat taagcaatcc ttccagcaag ctcttaaaga aagcagcgat attatagcgg
    12421 aaaagacagc cgatacaatg gaaaaatcca tggctaatat ttatcagatg tccgaatcct
    12481 ttgaaaacaa tcagaagatt ctggcacaga ccatagcatc tttaccggaa cagaccatgg
    12541 tatacaacaa atcggtttcg ggaaagatac agaagaaact ggatgaaata gaaaaagcca
    12601 taaagaatga ttaacataag ataagaaata agaggtgatc taattgaaac aaagagtgag
    12661 ctatttgaaa aaagtcgaac cggagaacta ttggccgtca tttgcggacg ttatgtccac
    12721 aatggtgctg ataatgctct tcttagttct catagtgttc attaaaaaca taataatatc
    12781 cataaactta gatgaacagg aagccactaa tattgccacc ggagctcagt tggaagcaac
    12841 aagaaacgat cttgcagcca aggaacttgc tcttcaggaa acacagctgg atctggaaac
    12901 aaaacagacc attcttatca tgctggaaga agaacttgca gcaaaagaaa tatccttgag
    12961 tctgttggaa aacgagttag cggcaaaaca ggccgcattg gaactgtcgg aggaagaagc
    13021 aaccaacctt caaaacctca tactgattct ggcagaacaa caggctgcgc tggaagctca
    13081 gaagaaggag cttctggaac tgatagcttt aaatacaata gagctggatt ccatgagaga
    13141 acaacttcag aaagtggccg ttttgcaggt ggatatattt gaaaaagtta agaaatccat
    13201 agatgatgca ttaggtccgg gacaagttac catcggagat aacgggaatc tgatgattac
    13261 ggagagtata ctgtttgatc aaggaaagtc ggacctcaag ccttcttcgt taaatcttat
    13321 aaaaaaattg gctgacgcat ttgaggaggt attaaaagat tcgggtgaaa atattgaagc
    13381 catcatgata gaaggacatg ccagcagtga aggagatttt gatctgaacc aaaaattatc
    13441 cacagacaga gccaatgcag tgttactgga attatttaaa aagaatggta aacttcagac
    13501 acaatatgca cgttacttcg gagcagtcgg ttattcaaac tccagactgt tgattaaaaa
    13561 tgactatttg gaatcacaga aagccaaaaa cagaagaata gaattttcca tagtagtacg
    13621 ggaagaaaat atagggcaga taattcagga gtacctggac tcgattgaca agaaaaagta
    13681 ggagatacaa tggcaagaaa agattttaaa gatttaaagc tgtatttttc caattccatg
    13741 ataagcctca aagaaggaga ctatgagcat gccataaaag gtttctccaa tctcatagac
    13801 cacggtatcg agcctcagaa atccgttata gggttgatta ccgcctattc ctgtcttacc
    13861 agatatccgg cggcattaaa actttatgaa aagaataagg atatttttat agataataag
    13921 ccaaacagaa atatgcttgt ggaaaccatg acaacccttc tcatgaagga aacaagtttg
    13981 ttgaagaaaa atgcacgagg atcgctttcc gccgtattca tggctaaacg catgaaagcc
    14041 gttcatgaag cataccttgc ggataaagat aatcttttgg ctatcattct catatgttac
    14101 tggtatgctg ttttgggggc aagaccgtat gaaacggaac agatgatgaa ggattttctg
    14161 cacaatgaat atgtcgatga cgaattccgt tggaaacttt tggagaaact ggccattacc
    14221 gacaaggaac ttatggatga tataactatt gcgggtatgt tcaggagaat accgagatat
    14281 ctggatcact cctacataaa cctgcttcta ttctcccacc tctgcggtga tgactttgct
    14341 tcggcaaggg agaagattga agttcagagg atgaacggag tggaactttc cgatgacgta
    14401 atgtggaatt acatcaattc cagcgtggaa aacaacgata ttgacgattt atcggtcaac
    14461 ttcgcaaaac gtctttttgc aaaaggttgg atggatcccg ttatcgggca ggtttttcgg
    14521 tatgccaaaa acaatctgaa tatctataac gtaaccaacg agaccaaggc attggacctt
    14581 ttcggtatat gattgtttta ttaaacttta tagcttaccg ctaaaaactt attaagcgat
    14641 gaataatatt acagacagct cagtatatca acaaatgtat tgagctgttt ttcatagggt
    14701 atgttttctt ccgagggaaa atgaataaaa gagatatatg ggtatgcctg tacggtacct
    14761 ttatgcaata acctgtaaaa tacatcattg catacatatg tacccgccga ataggatttc
    14821 actacaatat ccgttttata tacacattcc ggatattcca gtgttgcggt taatgccgtt
    14881 tccgccccgg gaattattac actgtcggtt tttgttactc cggcattgtc agctgcattt
    14941 gcatgattta tattcaacgc gattctttcc gtggttataa ctttccttgc tgcggctaat
    15001 ccggtagcaa tgacaatatc gggacgcact ttatttatct ctttgtcgag tacatcaaac
    15061 gccgtcttat atgttacatc cagtatgatt ttatatatct tatttccatt gataacatct
    15121 ggaagccctt ctaccagttt ccttgtaata ttgtcttgaa agtttgcaaa aggcttaaac
    15181 ccagagataa gtatcttcat tttaacggaa caagttttaa agcattttca aagtacagct
    15241 tattcttcac ctgctgactt aaatccagtg attctaaaaa ctctctgtgc ttggaatcaa
    15301 aatagtctct tgcatacaat attctgtcct gatattgatt caaaaaccgt aaagcatgct
    15361 cgggatctct tgttaaagca ttcaaaccgc ttcccgccga catatcgcag taaagattat
    15421 catacttgtc cagtagcttc tgaatcatgc ctccttctat gaccggtcct ttcggataag
    15481 ctactttgtc atataggtca tcgttggata tatgtgccca gaaaccggga gcgtgtccta
    15541 aaaatatagt ctcaggacat tttttaagca ctctttccaa agtatctatg ccgcctccgt
    15601 accagaaatt gggccagccg cttatattgg atctgggaaa ctcataatca aagtgcatta
    15661 taaccggtag ccccagctcc cctgctgccc tgaataatct tatagcatca aaatcgtcat
    15721 acatcattct gactttcagt tctccgcaga cctgtacttt gtgtatatat gtcgccgctt
    15781 tcagattctg tattgcatcg ggatctctcg gatcgggaca atatcctaac acaaatctgt
    15841 ggggataagc cctcttatat gccaataccg cttcaaaagg taccggtccc tttttattat
    15901 atgaagccaa tgctccgtta tagttttcat attcgtgtct cggacactcc catgaaagaa
    15961 tccatgtgac atcaatatca ttgtcatcca tgttcttcat aaacccgtcc aaatcggtct
    16021 tgtgccagtc ggcatggtta tgagcatcaa ttatcatttt tccgcatctc cttctttcga
    16081 tagattcagt ataccatcat tcttaatctc ttttatgtcc gatttgcaat atttcttgat
    16141 ttcttccaac tcgtagttat caaacggcgt tataagcgaa accgctatgc cgtttttcat
    16201 ggctctgcct gttctgccta tacggtgtat atactgctcc ttatcatcgg gtaaatcaaa
    16261 gtttatcacg gcatccacat cggtaatgtc cagtcctctt gcggcaacat ccgtagctac
    16321 taaaatgttg agtcgctcct ctttgaatgc tcttatcgtt ttttctcttc cggcttgcga
    16381 cttgcctccg tatatacagt ttgcagagta tcctttttca cgcaaatcat cggtcaacat
    16441 cctgaccatt cttatggtgt tgcagaatat gagcacttta tataactcat tattgtccat
    16501 tattgtttcc acagctttaa tcttgtaacg catattttga acggttacat acatctggtc
    16561 aatatccggc ttgtccaggt tatgttcttc tacggtaatc tccaccggat ttctctgata
    16621 aagccaggat atatccataa cccctatcga catggtggca gacagcagtg caagattttt
    16681 tctgtcgggc atcatgttaa gaatatcggt cacttcttca ataaatccca tatccagcat
    16741 acggtcggct tcatccagta ctgcagttct gactttagat atatccacag ttttgcgttc
    16801 gatatggtct cttaaccttc ccggagtggc aacaataatc tgcggatgcc ttttcaatgc
    16861 ctttatctgt ccttctatgg cttttccgcc gtatatagcc tctacccgca ctttgggcat
    16921 aaactccgac aattccttta attcattgca tatctgcaaa accaactctc ttgtaggtgc
    16981 cagtataaga gcctgtatgt ctttgttttc attatccagc tgttccagaa gcggtatacc
    17041 gaatgcaaaa gtctttcccg ttccggtggg tgcttttgca ataacatccc tgtactgcat
    17101 aagaggagga ataacacccg attgaacggt tgatgaataa acatatccct ttttattcaa
    17161 cgccttcatt attccttcgg acaagtgcaa atcggcaaac ttggttccgg aataattcag
    17221 tacctgattg tccttctcat gctctgcagg tcgttttctg aatttagtcg gttgtccggt
    17281 ctgcttatcg tattttctct tttttttgta ttgccctttt ttcttaaaag tattttttct
    17341 tttgtttcta ttattatcgg ttttatacat attaccttct gtttctcaat tcttcttcta
    17401 tcttatcaag agttatacct ctttccacaa gcaataccat aaggtggtat acaaggtcgg
    17461 atacttcata tacaatttcc tgctcagagc cgtttttcgc agctataatg gtttccgaag
    17521 cttcttcacc tatcttcttt aaaatcttat ccagaccttt atcaaaaaga taacaggtat
    17581 acgatccttc tttaggattg ctcaatctgt cggttattac ctgatataac tcattcaata
    17641 tctcattcat ttttctttat ctctccgttt tgctctatct gcctgtagaa acaggactgc
    17701 tcgccggtat ggcatgcatg ccctttctgt ttaacaagat aaatcaatgc atcaccgtcg
    17761 cagtccacca gcactttaag tacctgttga gtgttgccgc tggtctcacc ttttacccat
    17821 tcgctgtttc tcgaacggga aaaatatgtc gcttttccgg ttttaagcgt tttgtcaatc
    17881 tgctctttct ttgcatatgc cagcataagc acctgtttgg tatcaatatc ctgacatata
    17941 accggaatta acccgttttg gtcaaactgc aattttagca tccagaattc cctttaagta
    18001 tgcacaactt attgctatgc tcttttcctt aggcatatca aactgttcct gctccagaat
    18061 acaccagtcg ataacatcgg aaacattcaa aatagataca aagtctatca atcctcttcc
    18121 cagttcggtg tctttacctt cttcggatat atccttaaaa tgcagtgtat ccaatctgtc
    18181 tttatacttt ttacaatatt ccagcacatc caccccgcct ttaaccgccc agagtgtatc
    18241 tatctgaaga aaaatatcct ttacggtatt ttccgcaagt atgtcaaaca gcctttttcc
    18301 ttcatcaata acggtaaatt caaaggcatg attatgataa cctattttta taccgtattt
    18361 ctttgcattc tcgctggcta tgttcagata ttccgcaacg gttttcattc cgtccgcgga
    18421 atggaattgt tccgacaaat acggaaccgc cacatacttg ttgcctataa cggagttgta
    18481 ctccattgtt tcgtggaatt tatccacaac ggcattcaag ccggaatggc ttcctgccgg
    18541 cagtaatccc agatcatcca ggtatttctt agtttcaata gcttccttgc cgtaaaaacc
    18601 ggcaaattcc actccgtcaa agcctacctg ctttgcaatg tgcatggcat gataaaaatc
    18661 ctgttctgcg atatctttta tggaatatag ttgtagagct gctttcggca ttatttcctc
    18721 ctatcagcaa ttatacacat taaccgatac catttcaaat tacagtaacg atatcttgta
    18781 cattactatg cctgccgcac atgccgcatt taacgattcc gtcttacctt gcataggaat
    18841 ggacaatgtt tcggtaaggt actttcttat ctttttatcc aacccgtcgg attcattgcc
    18901 tatgaccaat gcacaacgtt tatccaggct gaccgtctca atattatgac cgcttgcatc
    18961 cagtccgtat atggcatagc cggcattttt aagtacggta aaaatctctt tgcttacctt
    19021 tatatacgga actctaaaca gcgaacccat gcttgccctc aaggattttt cgttatataa
    19081 atcggcacat ccttccagtg caaacacgca acccattccg aaagcttcgg cactccttac
    19141 cattgttccg gcgtttttcg gatcgtttat cctgtccatt acaagcaccg gattaccttt
    19201 tttaagcaca tccggaacat cgaatttagg aggtattccc agtgccagaa ttccttcggg
    19261 acttttcatg taggacaacg actccaatat gccgtctctc acaagataga gattcttaac
    19321 ggtaagcttg tccacataag acatattgtt ctgataatat gtgtcgctta catacacttc
    19381 cgttattctt atacggctgc ttaacgcttc atccagcagt tttctgcctt cgatgaaaaa
    19441 aaggtcctca cgattgcgga ccttttttct atccaagctt ttcagccttt tgattttctt
    19501 attgtccccg gattctatgc ggttcatggt ttacttaacc gaactgacaa gcgaagcaaa
    19561 ggctttcgga tcgtttacgg ccatgtcggc aagcactttc ctgttaagtt caataccgtt
    19621 cttcttgaga gccgatatga atctggaata agtcattccg tttgttcttg cagcagcatt
    19681 tattcttgca atccagagtt ttctgaaatc acgctttctt gctcttcttg caatgtatgc
    19741 gtaattgagc gatttcatta cctgttgttt ggcaactctg aaattcttac tcttatgtcc
    19801 gaaatatccc tttgcgagct ttaatactct tttatgtctt ctgcgttttg ttacgccgct
    19861 ttttactctt gccatttgtc tgttcctcct tacttgtacg ggataagctt cttaattgtc
    19921 ggtgcatccg catccgatgc gaaagccgtt gctctgttat cagtttttgc tctcttggtc
    19981 ttagatatca gtctgtgcct tctgaaagca tgtcccattt ttatcttgcc gcttgcggtt
    20041 actctgaacc tttttttcga agcgctgtgt gtttttaatt ttgccatatt ctaatcctcc
    20101 tattgtttct tgggtgcaat tataactatc atatgtctgc cttccagttt cggatctttt
    20161 tccggtgtgc cgtattccga acaggcttcc gtaaacctct ccaatacttc cgctcccatc
    20221 tgcgttcttg tcatttcacg tccccgaaat gacaggctta cctttacttt gttgccgtcc
    20281 gcaaggaact tgcaggcatt ctttatctta aaattaatat catgctcgtc tatacccaac
    20341 ttaagccaga tttctttcag ttccatggtt ttctgtttct tttttgcttc cttttccctt
    20401 ttggagatct caaacataaa tttgccatag tccatgattc tgcatacggg cggtctggca
    20461 ttgggggaaa tctcgaccaa gtccagtcct gccgcttcac tgtgcttgag cgcttcatta
    20521 accgacatta ttcctatcgc cgttccgtcc gttcctatta atctgacttc ttccgctgta
    20581 atattcctgt tagttctgta ctggcttctg gtaccttttt gagctgctat tgtaattacc
    20641 tccgtaaaaa ttataatggt cttgcagaca ataatccgca agaccacaca acaagttaat
    20701 gcgatatgtt tacccgttca gtcgttactt aggggtgaga agggattact tctgctttcc
    20761 gtgcgttata ctatcatatg gaatataagt tgtcaaatag aatttttcat attaaccgta
    20821 cacattatat tccgtatcta tcgtttctcc aggaatatat ccatgttccg gcttgtattg
    20881 ctctgcacgg taaaggtatc ggcaggaaga acggcaagtt cccttttcaa gccttcacgc
    20941 aacggcataa gctccgactg cttcatcccg gctatattga gtatcttgga attgtctatg
    21001 atacggtcga acatacggtc atattcaagc tgccagcgtg cataaggact gtttttatcg
    21061 ggtgatataa aggaaatata gtcttctttg tcaacctcct catatttaag ccctataagc
    21121 tctttgtaat aatccgcgac aactctccac ggattatgct cagccgttgc aactgtatat
    21181 gtctctcttt ttgcagcgtt attgaaaagc agtcttgaaa tcattttagc gacatctccc
    21241 gcccaactca tggttgcctg aacatccatc gcctgacgcg gaagcagcac cgttttaccg
    21301 tgtcttgcac gttcaatcac cacatccgct tcaagcgtta caagctggaa acgacgcttg
    21361 gagtaggtta tcgccgggcg gattgttgtc cagttgtcaa ggcctgacgc tttcaataca
    21421 tcctcgccgc gcgctttata cagcgaataa tcctccgaac tgatgaaagg ttcttctttt
    21481 ataacatcca gcagccgggg ggatgtttct tttatcggat gctcctcgtt tgcatatact
    21541 ctatatgtgg aaagatagat gtaatgaccg gtattttcca gcagcggacg gtatttttct
    21601 ttgaactcct ttgtcccgta tatcaaaaag tctactatgc cgtcgtaatc gtttttaagc
    21661 agctcaccga tatatgccgg ttcttttgcg ttcttggcct tcataaactt aatgttaggg
    21721 ttttcggatt gtcctccgtc aagtgccacc acatccacct tataacccat ttttgcaagt
    21781 aacggtacga gatatacgcc cattgcaccc gttccgccca aaactaatac cttttttgac
    21841 atttccattc ctccccgctt accgacagta ccactttgcc gacatgtccg ccttctttta
    21901 atattttatg agcagcgtcg gcttcttcaa tcggcagtat tttataaatt aaagttgtaa
    21961 ttttcttgct ttccggcagc ggccggacat ctttccatga aagggattta ctgttatcgc
    22021 tttcatatgt ttttttctcc gggaatattt tcgtaattca tcatcagaag cagacgcttg
    22081 gcatccttga gtttgggctg accttcagga ctgtcggcgc tttcctgaac gaccagataa
    22141 atatccgagc ctaaaagcgg tccgagcaga cgatgcttcc tgcttgcgct gccgccgcac
    22201 aggaaaagtg aaggaattcc cagtttatct ttcagacagg tggaaattct taaattttcc
    22261 agcaattcgc tttcggtatc ggctgcggtt actattttgg caatgtccgc gccgcgtctt
    22321 cgctgttctc ttgcaatttc cagaacttca tcggttttga tgaatttcag tacatggctt
    22381 gacataatca cctgtccgcc gagcttatgt acttcttcta tgaatctgat ctgtttttcc
    22441 accgctgccc ggttgtatgt tatctcactt gaagtcggat agaacatatc tgctctgatg
    22501 tctataagat cgccgccgca ttcaagagcc gtaatcagct ctttagcaag atactcgtca
    22561 tcagctgcac ttttggtatt gccccgtctg taattggtga cataagccgg tttgtcttcc
    22621 attgcgttga aaatatcgca aaaatccgtt tcggttctgc attccggcaa cattgattcc
    22681 atctgaagac cgaaagcatc gcatccttct tccagtgcga gccttatatc acgtattatt
    22741 tccgatgtca cccgggattt aatcattacg cagagcgtcg gtcgtcccgg gattaagaat
    22801 tttctgtgcg tgtgtttatc tctcatttta tcctccgaca atatcataat acggtttttc
    22861 attatacgct acatgcatcc gaccgatttt atttgtttaa caccgtttta ttatttcatc
    22921 ttcacggaga gttgtaaaga accggtaatc gcctaatata tatttacaaa tattttactc
    22981 tactatttcg tatagtgacg atgcatcacc ttttttaaca ttatccgata tatcggtcac
    23041 tctgaccttt accgaactgt tcacaaattt tatctctaaa atatcacctt ttttaacctg
    23101 ataagaaggt ttgaccgctt taccgtttgc gaatatcttc tgtgcttcac atgcatcatt
    23161 agctacggtt cttcttttta tgaggcggct tacttttaga aacttatcta ttctcatttt
    23221 tagcctcgtt ccagtacttg tccagctgct ccaaattcat ctctctcata tactgacctt
    23281 tatcgttaca actgttttcc acatgcgaaa aacgctttat aaacttatcc gatgcggcat
    23341 ccagactttg ttcgggatct atatccaaaa atctgcagac attgacaacg gcaaaaagca
    23401 aatctccggc ttcttccttc tgcttttcgg tatcttccgt ttcaatcgcc cgttttaact
    23461 catgggcttc ctcatatacc ttatcgaaag ctccccaaac atccggccag tcaaatccca
    23521 ctctctcggc tttactctgt atcttattgg ctctcataag tgcgggaaag gtcttgggta
    23581 tgtttttcat actgtccgct tcgctcttat agcctttttc ctttatcttc agcttttccc
    23641 aactttccag tacctgctcc gctgtatcca tctttttgtc accgaatata tgcggatggc
    23701 ggaaaatgag ttttctgcaa ataccgtcgg taatgtccga taaatcaaat gttccgtttt
    23761 ctttacctat ttgagcatga aataccacct gaagaagtac atctcccagt tcctcagtca
    23821 tcttttctat gtcattatca atgattgcat cgcatgtctc ataagcttct tcaatcaggc
    23881 atgttgtaat ggactcatgt gtctgttctt tatcccaggg acatccgcta tcgcttctca
    23941 gttttgccat tatttccaca aggtcgttaa aatcgtattt tttatcgtgt ttatccatct
    24001 tctacaccct tccgaatagt cattcgcttc ttgctattac tattctatca gtattatgta
    24061 aatctttgtg taccttaata tcggtatatc cgtacttttt cagtatatcg catacccgtg
    24121 atgcctgatt atatcctact tcgtatagga tatatccgcc ttttttcaga tacttcatcg
    24181 catcggcgga tatcaattca tagtactcgg ttccctcctc acctgccagc agtgcaagct
    24241 tgggctcata ctccagcacc tgtttgtcca atgcgacata ttcatgtaac ggaatatacg
    24301 gaggattgga tactattata tcgaactgcc cttcaatttt attaaaaagg tcgctttgta
    24361 ccgccgaaca gttacttatt ttattaatca atatattctt attcgcaatc tcaacacact
    24421 tttccgatat atcgctcatg ataacttttg attgtgcaag ttcctttgct acggatatac
    24481 ccgcacagcc gcttccgcat gccagatcca gaatccgggg tgattccata gttttaccta
    24541 tggctatggc tttctccacc aatatttccg tgtcgtttct gggtataagg acatgcgaat
    24601 ccacataaaa attattgccg taaaagcagg ccttattcag tatatattcg aaaggttggt
    24661 ttttgcgcct tctttccgca tattcaagca gcagtcggta cttttcagta tccacaatct
    24721 catccgggtg tgcatagaaa tatgaaatat ccttttggaa cacttcttca agaaaaaaat
    24781 acatttcctt tttatcctta aatatttccg aacaataacc aatcaattgt gaaattacca
    24841 tttcgcttct tccggatctt ccggtactac cgcattgaat gccattatgg cgcattccac
    24901 catgctttca tccggttcct ttgccgtaag cttctgaaac agcatgccgg gagcggatac
    24961 aattttggaa agcacattat catatctgcc tgctacttta acaagctcat atgccaatcc
    25021 cgccaccagc ggaagcaaca gtatcctgaa aaccatgttg agaatagccg aatgccatcc
    25081 cacaagagaa aaaacgatta tggacaccgc catgaccaga aacatgaaac ttgtcccgca
    25141 tctgcggttg cttgtaggat acttcataac attttccacg gtaagttcct catcagcttc
    25201 atagcagttt atggtcttat gttccgcacc gtgatacatc cagacccttt ttatatcctt
    25261 cattaaagtg acggcagcaa ggtagccgat aaaaatggca attctcaata cgccttccag
    25321 taaattattt acaacggcac tctctatctt aagaagacct atcacgaaat tgggcagaag
    25381 tataaacaaa cctacagtta ttgccaccga taagagaaca gcaaagaaga tggctgtctt
    25441 actcaactca ccgtccgatg attttgtttc cgctttcttc tcatccggtt gctctttatc
    25501 tttctcattc tgttcatcgg tcttatcaat atcctcttct tcaatatcat aaaaagatgc
    25561 cgcataagac aatgccttta tgcttaagac catctgatat atcagtgaat aagcccctct
    25621 taatacgggt atttttgcta tcttccactt tttagggaga ttctccacat ccagtactat
    25681 atcaccgtcg ggttttctta ccgcgattgc ttttttatgc ggtcctatca tcattatgcc
    25741 ttctaacaat gccgaaccgc cgattgatgt ttttctcata tcataaaaaa atcgggttgt
    25801 ttatcagaca acccgccttt cctttaaatt acgaaatacc gtatttcttt ttgaatttgt
    25861 ccactcttcc gccggtatcc acaaatttct gtttgcccgt gaaatacgga tgacaatttg
    25921 aacaaatttc cactttgata tccttccttg ttgaacctat ttcgaattcg ttcccgcatg
    25981 cgcaggtcgc tgtagtttta tagtatttcg gatgtatatc ttttttcatc cttatcacct
    26041 cctaaattgc cgcaagcgtg attataccac actgtataaa ccatttcaac agtaattttt
    26101 aatgtaccta tacctgattt caatcgtaaa ttgaaaaagt aaattccagt actaataggc
    26161 tcaagcggat tttactcttg cactaagttc cagttgttga tttgcataac aaaaagccat
    26221 atagtgtctt tctgaaggtt gctcgtttgg aaaggagcat ataggatgag aaaaataaaa
    26281 tgtagcaaca aacatttgac acttgatgaa cgaaaaatta ttcaaacagg tattgaaaac
    26341 ggatccacga aagccgatat agcaagagtg ataaataaag attcttccac ggtggcaaaa
    26401 gaaatcagaa aacatagaat aaagaaacct cgtaacacat acaacagacc aacgatatgt
    26461 atacatatta gagaatgtgg taagagatgt ataggaaact gcgaaagata tgaagagcct
    26521 aaatgcagaa gaagagatgt atctcccggt gcatgcaata aatgtgataa atatagtaaa
    26581 tgtcatttag acagatatat atacaatgca cagcgtgctg atgaggcata cagacaaaca
    26641 ctcatagaat caagagaagg aattggaata tcctcaaagg aaatgcttag aataggtaat
    26701 ataatagctc cgcttctaaa gaagggacaa tcagtgcacc agatacttgc agctcataga
    26761 aatgagatac gtttaagtga gaggactttg tatcactaca ttgaaacagg tgtgtttgaa
    26821 acattcggca ttgactgctt ctctctcaaa gaacaggtaa atagaaagcg ctttaaggga
    26881 aaatacaagc caagaaaaga aaaagctgac tataccggaa gaacatacaa tgatttctta
    26941 aacctgttat cggaagatgc tgaaatctca tatgtggaga tggatactgt tatgaattct
    27001 ttgagtggtc cgtatataca gacaattatg cacaaacgaa ctaaactaat gataggattc
    27061 atacatcgtg aaaagacctc taaatcaatg tcagatacat ttgatatgct tcagcaaaag
    27121 cttggaagaa acctttttac tgcactcttt ccggtggtac tcgctgaccg cggtgcggaa
    27181 tttgagaagt atgagatgtt tgaatcggat tctgacggtt taaccagatg cagaatgttc
    27241 tattgcgatc cgatgcagtc aagccaaaag cctcatgtgg aaaataatca taattatgtg
    27301 agagacataa tacccaacgg atatcctctg gataaaatcg tacaagaaga ccttgatatt
    27361 atgttctctc acattaactc aactccgaga aagtctttgg ctgataaaac accatatgaa
    27421 atgttcgtat ttctttatgg taaagaaact gccgacctac tcaatatgag ttgtatagag
    27481 agggatgaag tcacactaaa acccaccctt ctaaaccatg tctataagag gtaaggagcc
    27541 tcttggacat ttaaccttcc cgtaaaacgg gataaaacga gctaccttca aacataaaaa
    27601 ttagcatttt acagaccgga ctttaattgt gcaatttttt gcaaatgatt aaaccggcct
    27661 caatttcatc atgcctttct tttgttcagg caaagataat atattctctt ttcactaaaa
    27721 aaacaatacc ttttttactc caaagaagaa attctgtcca tatttatgga attttcttgt
    27781 gcaaaaaatt ttttctcttt agtactggaa tttacttttt caatttacca cctgatttca
    27841 atcaataatc catgacagcc gtctgtttta tattaatgct gctgacgaat tccgcgttat
    27901 tcctggtctt catcagttta tctatcatcc attcggttac ttcggcagta cccatattac
    27961 tcatggcgcg ccttatggtc caaatacttt ccagttcttt cttgcccagc agcagttcct
    28021 ctctcctggt tcccgacttg ttaatgtcta ttgcgggaaa tatccttctt tcggcaagct
    28081 ttctgtccag atgtaactcc atattaccgg tacccttgaa ctcctcataa ataacgtcat
    28141 ccattctgct tcccgtttcc acaagagcgg ttgcaataac agtaaggctt cctccgaatt
    28201 caatgtttct tgcggaaccg aagaatttct taggagcatg aagcgctccg ggatccagtc
    28261 ctccggaaag agttcttccc gtaggtgtca ttgtaaggtt atgcgctctg gcaagccttg
    28321 ttatactgtc aagaagaatg accacgtctt ttccgtgttc caccatcctc tgagctcttt
    28381 ctaaaaccat ttccgatacc ttgacatggt tttcgggaac tttatcaaat gtggaataaa
    28441 caatctctcc ttttaccgac cgctgcatat cggtaacttc ctcaggtctt tcatctatca
    28501 gcagaacaat aagttccacg ttgggatgat taagagtaat gctgtttgct atctttttca
    28561 acaatacggt tttaccggct ttgggaggag atactatcat tcctctttgt ccttttccta
    28621 tcggagctat aaggtcgatt attcttgtgg aatagtcttt cggattattc tccagagtaa
    28681 gccgctcctc cgggaatata ggggtgagtg tatcaaaaga ctttctggta aatacttttg
    28741 acggatcttc gtcattaatg gttattacat tctgaagagc ctggaatttc tcattatctt
    28801 ttttaggttt tccggtaccg aaaatcatat cgccggtttt aactccgtat ttgcgaatct
    28861 gattgggagc tacatatata tctttaggac cgtatgagta attatcggta cgtaagaagc
    28921 cgtatccgtc cggcagcact tccagcaccc cgcaaacctg atcatactga ccgtcttcgc
    28981 attcttcaga taaatcgctg tctgtctgcg aatcggctgt ttcctgaaca cttgtactct
    29041 gcgcctgtcc gggagggaca atagtagccg agtcatgtga ctttattaca tgcctcgttt
    29101 ttctctcctc tttcacaggt tttgcaactt ccgattcttt tgccatcttg gacaaaagat
    29161 atctgggctt cttgggagtt tcctgtgtta cggcttcaag tatttttgcc gcctcggaga
    29221 cagcaacttc ttctttcggt tgctccttct caatatccgg ttgctcctgt ttggttatct
    29281 ccaaaatcct ttcaatcaat tccgcctttt tcataaccgt gacgctcttg acgcctaacg
    29341 tcttggcaat aacccgcaaa tccgcaatgg atttagactt taaaatctca acctcgttca
    29401 ttaatatcct cactaaagta tttttttata tttagagtat agatttgttc tttccgaatt
    29461 caaacaatat catacagcaa gtactttgtc aaacatttct tgtatatttt gactatattt
    29521 ctgtcagcaa ggtatatcgg tcacaagtat ttcagttata cggaaatatg tgcaaacttg
    29581 tctatatttt actctttttt attacttctc ctatgttgtc atagatatat ttcggtctgt
    29641 aagaaaagtt ctgaatattc tgatggttgg tcactccgct taaaaccaga atggtatcta
    29701 tttcactttc tatacccgca actatatcgg tatccatacg atcacctatt attatggtat
    29761 tctttacatc ggtccccagc ttcttcaatg catgcctcat catgagcgga ttgggttttc
    29821 cgatatagta agcttttttc ccggtagcta tctcaatcgg taccgctaac gctccggttg
    29881 cgggcattat tcctttttcg gtaggaccgg ttctgtcggg attggtacct ataagttttg
    29941 ctcccctttg caccaactgc accgcttttt ctattttctc aaaagaataa gacctggtct
    30001 cacctattat tacatattta ggacttacat cattcattgt aattcccact tcgtataaag
    30061 cgtttatcag cccgggttct cctatcacat atgccgtaca tctgggtgac tgtgaggcaa
    30121 ggaaaagtgc cgttgcgatt gccgaagtgt aaaaatgctc gtcacctaca acaataccca
    30181 ttcttgcaag tttctgtgaa agttcacgcg gcgaacgttc ggaactgttg gtcagaaata
    30241 aatacttttt gttattgttt tgcaaccaat caatgaattc cagcgctccg tccaataaat
    30301 tattaccgtg atataagaca ccgtccatgt caattatgaa accgtcttta ttcttaattg
    30361 tatcgtccat ttttatctcc tgtctttctt gattttctta cgtctccaca gtatcaggta
    30421 tatgaaaagg ttggctataa ggtgctctgt ggttgtaatc aggttgatgg aaggattatc
    30481 cttatcaatc agaacctgtc ccaaataaag ctttttagca gcctttccgt taaaccttat
    30541 tctgttttta tcccatattt tatctatcag attcttaaaa aagttattct tttctttcat
    30601 aatctgtctg tgagccgatt tagcggaacg gtatgcagcc attatctttg taatgagctg
    30661 ttcaggtgta taggccctta ttttctctat accgaacatg ttggcggtaa tctccattcc
    30721 cgcgaaaaac attctttttg cggtaagcac accgtcggta ttgtttctta aagttcttaa
    30781 aagagaaaac tgcagcaccg attccgttac aaatcttttg cttttcttaa cattcagata
    30841 aagcatttca atttctctgt cggtcaaagg ttttgcatag tcatgagttg cattgtcgga
    30901 aatatagtaa ttctttccga taaacttacc gaatgctttc atgccgtcaa gatatcccag
    30961 caacatattt cgggctgcca attcggggtc gaattccaat atacctccct caatctgctg
    31021 attctctata tacaatatct tagcatccga ttgctgaaca cgcctgtata cgcctatggc
    31081 ggcattgctt accgctatta tatttttata accgtgctca atcagtacat ttataggaag
    31141 attatcgtag aaacctccgt ccagataaag ttccccgtct atttcctgct ttttaaatcc
    31201 gggaacggcg gaacttgcaa gtatgaaatc cactacttta ccctcaggaa tatctttttt
    31261 aaacagtatg acgggtttca tctttgttaa ggaaactgtt accaatgcat aatcaacagg
    31321 tgaattacgt aagacctctt catctattac ctgctccaga tagcctttca atgtggatgt
    31381 atccagccct ttattcttaa gtatggattc gatatactcg acagaacccg gctctccgtc
    31441 ttctttgtca taggtaatat caaagagagt gggcatatcc aatgtcttcc ataggtattc
    31501 cgcctgctca aatgcaccgc agacaaaagc cgccccgttt atagagccca cggaagtacc
    31561 tatgaccatg tcaacctcta tattcagttc tctcagagct ttccatacac ctatctggta
    31621 ggaacctctg gctccgcctc cggcaagcac aagctgatat ttcaagtcca ttacctaccc
    31681 ggattgatta tatataacct gaaactattt tacaattgta actactgaaa tttcttgatt
    31741 tcgagattta tacccgcttt ttttgcatat ttaaacatat cgtttatctg cttggtctga
    31801 tggccggtga gtttggcatt gggaatgacc accaacacgg ttttctccct tatatcctct
    31861 ccgctgaccg aataaccttt gtattctccg taggtaaact tatccatgga atcgatatgc
    31921 ttttttattt ttctcttcag ttcggtaccg gacttatagc tgtcttcctg cgtatttata
    31981 cttacgatag ataccgcctc ttttgtataa ccgtcatata tatccagagt cggaaaatta
    32041 tgatccagat tggctcccat cattttatgt attacctgac ctcgttccaa agggtccaat
    32101 tcccagatat tttctttaac aaccccttta ccgtctcccg cccatttaga tgataccgaa
    32161 cgaacataaa ctctgttttt cttcaatatc ccgctgtaat cggcaaagac cgagagagta
    32221 aagaagtcat catacggaac cgcaaagaat attgcattgt ttatatccgt ctcctccggt
    32281 tgcgtaataa catccacaag atatattccc gatgcatggt atgcgacggc tccattgaca
    32341 agtcgcctgt ccagttcgga cagtaaatcg gaatgtgcct ttatatatcc gttcagttgt
    32401 atgctcaaca acagtacaaa tatgagcaca aagcaacata tacagctttc cgccgttatt
    32461 ctgccctcag tatgagtctt ccgcttcaac ataaaaactc ctttcggtac ccaaaagacc
    32521 tttgaacttt aaagttactc ccaccttgat gccgcagcac accgaagaca tgtccatatt
    32581 gtccggtgaa ttgagagcaa tcagatccag cagccttaag accttgacat tattgctttt
    32641 tgtgcataaa agaagccgta aatagtcatc ataatcagtt ttcagccagt cgggtgtttt
    32701 acttaaaccc ttttccagac ctatatccag attccagtca ccgtccatct tgattaccgg
    32761 acacttcgct cctctgcaaa gagcctcggt atcgtatgcc gattccgcta tagcccagga
    32821 acataagata agattcttgg ctatgggtgc tgccgcaccg aaagtccagc tgccagcaag
    32881 acatgccgcc aatgcatcgg ctttggtagt cttaggcaaa tcggtataga catgaatggc
    32941 gttacaagca aatctcaatg ccataagaga cgctttgcac gattgaacat tctttctgtt
    33001 gtctctgttt ccgaaaatga tatactcaat ttcgccatct aaatatctgt tctcaccttg
    33061 atcggtatcc gaatccgtaa aaccgttcat atacagcatt atgtattcgt taatcatgat
    33121 atcttcttta atttgagaca gcatctcgga taaatccgtg aaagattcgg atatgctttt
    33181 tgaggtgttg ccggtggcat cggcttggaa aatatatgta tcctcataac cttccgatac
    33241 cagattgata aaatcatcta tccttatgtc ttctattatg attgattcca gtacttcatc
    33301 cgccattttt tttaacaggg accgctgatc tttaactata tcatcaatga ccgatgcaaa
    33361 aaacccgtcc agaacatccc cgttatatac agcatcatag tacatttcaa aaccgtcaca
    33421 aaaaacatca tatagagaaa catccttttc ttcacttaca gtatccagca tatgcttcat
    33481 ggtctgttcc agacagtcaa catttctgtc caataccgct ttgatgtttt gcaaaaccga
    33541 caccgataag gaagtttcca ttttataaac ggtatctgca catatcttca atacggcctt
    33601 taactgttcg ggacaatctt caataccgtc cactatacct ttcagatttt ccgacagtat
    33661 gtgtatatcc gcataggtgt taatcatttc attcaacagg ttaacggctt ttttattgta
    33721 ctctttcaga acatatataa tattatccac attggttttc aaaatatccg ttacctgttt
    33781 tttcagcacc tgagccgtat ccgtctgctc cttatctccg cttttcatat aggaaagcat
    33841 ctcgccgtac aacgaatccg cgtaacgtat aatctccatc aggtcatgat aatattgact
    33901 ggaatttatc tcttcaaccg tttctttcaa caaagtatga gcgtttttca atccgcctat
    33961 caattcgtct atctgcatct tcacattaag agtcctgata acatccttaa tcttggaaaa
    34021 catttcaatt ctttcgtttg tctcggctaa aacggttctg aatatttcat cgctcataag
    34081 gcggtttatc tgagcggata tgaccggata acttgtcaga ggtttgtttc tctctacttc
    34141 cacattcact atttccattt caaaaagccc tctgcttatc gaagagttat tcagtgcctc
    34201 ggttatacgt aaagcagcct cccggtcgct catctcatag ccgtatagtg aataccgctc
    34261 cttcatgtat gtgctgtatt gactcaatac acatcctaag gatgctttag cggcattttt
    34321 cgccgcattc agtgcatttt tggcaagata tatatcggat agcaccaata ccaacagtat
    34381 cagcaccggc gttataaccg ccacaaatgc cgttatcact cctttttttc tatttgaaga
    34441 aggtttcaaa tataatatcc cccttacgct ttataccgtc atatgttctt atctcggaag
    34501 tcaggtcata ttccgtatac tcaatctcct cgcaacttat cttcacaatc atcatcatta
    34561 cggaaataac cagcataaga atgagaggaa gaactattgc cgcttccgcc gccgcactgc
    34621 cttttctttc ttttttaccg tataaatatc ccatggtacg atgataatat ttcatccggt
    34681 attattacac agacaaaact gtatatttac agaaactgta aatattattt ctatatgaat
    34741 tacccctata aaatgtgtaa tgctttgaaa tgtaataaaa ataagagaaa atcggaaaaa
    34801 aagtaaaatt cggataaaaa tatatatttt acctactcaa gaaacattaa taagtgtata
    34861 attgacataa atctgtagta aatattaata acgggaggta aatatgacat ttgaccctaa
    34921 ttccaaatta atgtacataa tggtagcaat agtcttactt tatgttaccg ctcaggcggt
    34981 ctttttcctt gtaaaagcta ttaaaagggc taaagagttg aatataagct catcaacggt
    35041 aaaaaagacc attctatcca gtgcaatctt cactattgca ccggcgatat ccatacttat
    35101 gggtgtgctg actatggcgg gctttttagg actgcccttt ccatggttgc gtttatccat
    35161 tctgggagca cttacatacg aagttaccgc ggcaacttcc gcagccaaat cgttgggcat
    35221 caccgacctg tccactttgg ttaccgatcc taaagttttt gccacaatgg catgggtaat
    35281 gcacttggga atattcaccg gacttattct tgtccccttg ttccttaaga aaattcaaag
    35341 aggccttata aagataaaag aacgggatga aaaatggagt gatgcttttc ttactgcact
    35401 gtttttaggt atgatatcgg cgtttttagg acttatcttt gccgacataa aatccggact
    35461 tattggatgg ataccggtat tcgtaatgat tgtttcggca ttgattatgt cggttctggg
    35521 tatactggtt aagaaattca agataaagtg gctggaagat ttcgctcttc cgataaccat
    35581 gcttgtcgca atggctcttt ccattcccat taccaatctg atagaaagtt taatttagga
    35641 ggcggacatg aaaaagaata aaacaacaaa atcttatgat gatcaaatac atttccacgg
    35701 aagaatctgg atgttgggag ctttagctct tttcacactg ttcccgacag ctatatgtgt
    35761 tatatacaac gcatggccgt ctgccggtcc tctcttcaaa gctcttgccg gagttgcccc
    35821 tattttctgg acagtgggcg ttattgaaat tctgacatat acccccatgc tgggctcggg
    35881 aggctcttat ctttcctttg taacgggaaa catctccaat cttaaggcac cgtgcgcttt
    35941 gaatgccatg aatgcatcca aagtaaaacc gggaacagac gaaggagaag ttatctccac
    36001 tattgccata gccgtatcca gcatagtaac catagtcatt attgctatcg gggtattggg
    36061 acttaccgca ataagaccgc tattggaatc ggaagcatta aaaccggcat ttgccaatat
    36121 acttcccgct ttattcggag gattaggagt agtatatata tctaaaaact ggaaaatagg
    36181 cctagcacct ctgttattca tgctggctct gttcattatc tcgcctatcg atttatccgg
    36241 ttatgtaggt gtactggtac ctgtaggagc aattattgcg ataatagccg caaggatatt
    36301 gtataagaaa gacaaacttt aggaggtatg ttatgtggtg gttaattttt cccgctctgt
    36361 ttatcatttt ccttctcatt cttatcattc gggcgttatt gtttaagcct tatccgataa
    36421 cgaaacccga aataactgat attgaattag ataatgaggg gataataaac agattgtcgg
    36481 aacttataaa atgcaaaacc gtatccaaca tggatttcga aaaggtggat tttgcggaat
    36541 ttgaaaaact gcacaaactc atagaaaagt tctatcccga tgttttcagg aaatgtgaat
    36601 ttgaaaaagt atcaaacaca ggtcttctct tccgctggcc gggtaaagaa agcgatgcac
    36661 ccacagtgct aatggcacat tatgacgtcg ttccggcaaa tgaggaccaa tgggataagc
    36721 ccgcttttga aggtattgtg gaaaacaatg aagtgtgggg tcgcggtaca ttggatacca
    36781 aaggtacttt cacctcaatt ctggaagcaa ccaatctcct tattaaacaa ggctttgtac
    36841 cccaaaacga catctatctt tctttctcag gcgatgagga agtgtccggt ccctcctgcc
    36901 ccgctattgt ggaattgttt aaaaaaagga acatagtacc cgcattggta atagatgaag
    36961 gcggagcggt agtggaagat gttttccccg gtgtaaaagc cagatgtgct cttgtgggta
    37021 cggcggaaaa aggagctatg gatgtacgta tgaccatgaa aagtcagggc ggtcacgcat
    37081 cctctcctaa acctcacaca attatcggtc agctttcaca agccgttgtc aatattgaaa
    37141 ataacccgtt caagttccaa ctgactcccc ctgttaaaaa aatgtttgat aacttaggca
    37201 ggcattcctc ttttgttttc aaagtaatct ttgcaaacct gtggtgtttc agtccggtat
    37261 tggatcttat gtgcaaaaaa agcggaggcg agcttaatgc tcttatgaga accaccgttg
    37321 cattcacaat ggcaagcggc agcagtgcgg ccaatgtact tcctcccaaa gcatccttta
    37381 ccgcaaatct gagacttata gggacagata catgtcaatc ggctatagaa tatcttaaga
    37441 agaccgtaaa aaatgacaat attgaatttg aaatgattca cggttccgac ccttcgatat
    37501 attcctctac cgactgtaaa ggctgggata tactgaccga tgcaataaaa cagacatggt
    37561 ccgatgcaat agtctcccct taccttatgt ttgcggcttc cgattccaga cacttcagca
    37621 agatatccga taaggtatac agattctccg caatgtactt aacaaaagaa caaagagctt
    37681 taattcacgg aaacaacgaa agaataccct gtgataccgt atacgatatg gttaagttct
    37741 atgtgagggt tattaaacag agctgagtgc accgattaac ataaaagaat atttgtctat
    37801 gaaaacgcaa attaccttaa aaatttgcgt tttctttcca tattttccat agcaaactca
    37861 aattttattg actttttgag ttttctatgc tgtaataccc ttagaaaact caaatttgag
    37921 gtgatattat gattaatcgt tccggtgaat atgtttccaa tctttcagga aagcttgagt
    37981 ataagtcctt cagacccgct aagcttcccc cgataccgcc catacaaccc gatgaggaaa
    38041 tgttggctct tctgctggag gcaaataaga aggtttcgct cttagacggc cttgctgcaa
    38101 gaatccccga tatggatctt tttgttgcca tgtatgtacg taaagaagca cttgtctcat
    38161 cacagataga aggtacgcaa tgtactttgg aagatatact tgaccctaac cttgatacaa
    38221 ataccaatgc tgatgtttcg gaagttgtaa actacatcaa agcgatggaa tttgccgtgg
    38281 agcagcttaa tacgcttccg ctctgtaaca gacttatcaa agaaacacat gctgttttgc
    38341 ttcagggagt acgcggagcg gaaaaaacac ccggtgaatt ccgtacatcg caaaattggc
    38401 ttggcagccc gggaagtaca ctaaaaaatg cccgctacat acctcccaat gtgcaagata
    38461 tgaatattgc aatgtccgac cttgagaagt acataaatag tgaagactca cttgatacac
    38521 taatcagagc agcactgatt cattatcagt tcgaaaccat tcacccgttt ttagacggta
    38581 acggcagaat aggaagattg ctgatcgctc tgtttttaat gcagacacaa acactgcgtt
    38641 cccccgtact gtatatgtcc tgttacctta aagccaaccg tatcgagtat tacgaccgtt
    38701 taagcgaagt ccgaaaaagc ggaaattacg aacaatgggt tactttcttt cttcgtgcta
    38761 tagttgaaac ggcaaccgac gctatagaaa caattgacaa gttaaccgag gtgaaaaata
    38821 taagcattca gaagcttgac gacataaccc cgagcatcaa agcaagactg gagacattac
    38881 ttacttatct tgagaaaaat cctataatag ataccaaaaa aacagctgag gcattaggct
    38941 tttcctataa ctccacagct aaatatgtag atatactatg taaaaaaggc attttggaac
    39001 attccagaaa agcaggtaag acaaaaatat attcttacac ggactatttg aatatcttgc
    39061 gtaaaggtac ttaattactt aatcataaac tgcttaatac taccttagag aatcaattgt
    39121 tctgtatatg gcttctctgc ctattctgtt atcatcaaga tagaaagtac ctgtacacaa
    39181 taaccatatt attttatcaa tcttgtatgc cgtagcattc ttatcagtgc cgcttttctt
    39241 tacggcttga gcccaagaaa acatctcttt aataacatcc tcttcattga gcttatcatc
    39301 agattgcatg tatctgacta cctttataat atgtacatcc ggtttagcca gccatgtgca
    39361 gccacaatcc ttcaaccaat cacaggttaa tgataaaccg agaccatgta ttttctttga
    39421 aattgcatgt aattccttta gttccttgtc tgtcatattt gatgttgcca caagatctgc
    39481 tattatcttt tcaccattat caactgttaa aaactgcaac ccgtcataga gtcctcgaca
    39541 gtatttttcc caattggttg ctgtatgctt tttcataccc ttatcaatga tacccatatc
    39601 aagcatcgca tcataaatgc ttgtccattc atgatatata gaagcggatt tcttcgtgtt
    39661 aaaatcgaac agtacctttt ttataatcat tctgttggtg gtatctttat cgttaaactt
    39721 tatggagttg cgcatcctgc cgctattctg taaggatgaa caaagtcgaa tcagaatacc
    39781 ctttgaagtg atatctcttt tagggtaaaa gtacctctga agagaaactt catctattcc
    39841 cattgctgca gcatagttga ctaaactgcc atacagtttt ttatatagtt caatttgcat
    39901 tttgcacctc cacatctcta cctattatta gagcatactt gtaataaaaa atccattatc
    39961 tttggacttt ccggctgttt gatatgtgct attccatatt tttctttatc cgatggatta
    40021 ttcctccata ctaatatata attagcagtg aactgtttaa tgatttttaa caatcctgca
    40081 ttaggaaagg acagattatg aaggtataca atacacttac aaagaagaaa gagcaactgg
    40141 taccgattga cgataaaacg gtacgcatgt attcctgcgg acctaccgta tacagttatg
    40201 ctcatatagg aaacatgaaa gcctttttat tcatggattt cttaaaaagg tcgctgaatt
    40261 atttaggata taaagttaag aatgttatga atataaccga tgtgggacat cttgtttccg
    40321 atgccgacga aggtgaagac aagatgatta aaacggcaaa aagtaccaat aagacgccat
    40381 gggaaatagc agcgatgtat accgatgctt ttcttgcgga tattaaaaaa ctgaacatac
    40441 aaagacccaa tgtactgtgt aaagcttcgg agcacataga agaaatgctg gaaatggtat
    40501 acaagctcta tgatgagggc tatgcatatg aaacaagcga cggaatatat ttcgacatag
    40561 caaaatttcc gcaatacggc agattgtcca atctggatct ggaaggacag cttgcgggag
    40621 caagagttgc cgtcaatgaa gaaaagagac atcctgcgga tttcgctttg tggaaaaaag
    40681 ctcccaaaga acacattatg caatgggaca gcagatgggg tatgggttat cccggatggc
    40741 atatagaatg ttccgcaatg agcagaaagt acttaggtga tcagtttgat atacataccg
    40801 gaggagtgga tcatataccc attcaccatg aaaatgaaat agcgcagtcg gacgctttct
    40861 tgaaaaagca ggcggttaag tactggatgc actgcgagtt tttacaggtt gacggaggca
    40921 aaatgtccaa atccatggga acagcctatc ttctgtccga cttggaagaa aaagggtacg
    40981 aacctcttgc cttccgatat atgtgcttaa atgctcatta cgggaaaaaa ctgaacttca
    41041 catgggacgg aatagaggcg gctcagaaaa gtcttaaccg tttatccaat ctcgttttag
    41101 aacacaaaga atcggatacg cacattgact caaacgtgat agaagaatac aaacagcaat
    41161 tccgtgaagc attgcaagat gacctcaatc tgcctcttgc catgagtacc gtatggaatg
    41221 cagccagaaa caatgtcaaa tccaaaagta tatataatct gctgatggat ttcgataagg
    41281 tgcttgctct ggatcttgac aaaaaaagaa ccgaagcggc ggatgatata ccggaagagg
    41341 taacactact tgccgagcag cgtctggaag ccagaaaaaa caaagactgg aagaaatccg
    41401 accaattgcg tgaccggata aggcagttgg gttattccat aaacgataca ccggcaggtc
    41461 aggttataaa gaagggataa aatgcttatt aaagtatttc agaaaggctt taatttctcg
    41521 caggacggcc cgggtaacag acttgtttat catctgcagg gctgcaacat gaattgcccg
    41581 tggtgttcca atcccgaagg tatagaaaaa gacggcagta taatggtaac cgaaggtgtt
    41641 gacgatgcag tatgtcctaa cggtgccata tcttccggta tgctggacag caaactttgc
    41701 aggggctgtc ctaaaatgtg tcttcagaca ctccacatgg gcattaaatt atcctgcgac
    41761 acataccaca ccgatgaaat agtttcagaa gccgcaagaa gtaaatccat gttctt
//
LOCUS       DAOSOM010000019        36481 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_1192_length_36481_cov_6.405013, whole genome shotgun sequence.
ACCESSION   DAOSOM010000019 DAOSOM010000000
VERSION     DAOSOM010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 36481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36481
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1192_length_36481_cov_6.405013"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="PLF14_05860"
     CDS             complement(<1..225)
                     /locus_tag="PLF14_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70855.1"
                     /translation="MNSYERVMAAFSKEKSDHVPIYCGSVSSRVASQVMGRTMYVGGG
                     IQQYRESLARFRGEQAHREFAEKYRQDAFDW"
     gene            complement(335..1153)
                     /locus_tag="PLF14_05865"
     CDS             complement(335..1153)
                     /locus_tag="PLF14_05865"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOT70856.1"
                     /translation="MKYLKPISLIVFILCAAVLMYVIITYETKVEYVVSGETIISCKT
                     NAKHPVIPASIDNKAVASIGNRAFCDNSSMITVKLDDGIIELQDESFYNCSALREVFI
                     PKSVTYISPTAFSECPEFAKIEVDQGNRIYKSFNGVLFKDGMSEIVYFPVSFMRTEYY
                     IAHGTRIIGEKAFYKSPLYTVHIPDTVTYIQDYAFAESSDMKSVTLPANLIYLGENVF
                     LGNGKLRTITIGAGVEIKPNSVDGISFYEQYQLYGAGTYLKTGNNIWEYIHDKD"
     gene            complement(1256..1873)
                     /locus_tag="PLF14_05870"
     CDS             complement(1256..1873)
                     /locus_tag="PLF14_05870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOT70857.1"
                     /translation="MVTLNFEKREKNLKGRQLRNKGIIPAVLYGKHLEESVCLQLPVH
                     EAERFLQYNFIGSKVEIVIGKEKYMAVLKEATYTPGANKLEHLSFQALVAGEVTRSVA
                     RVIISGRERIREGVLIHMLDEITYSALPADFVDRVEVDVSQMKPGDVLTVEELDIAKN
                     EKIELVTPPDSVVVTVTVPKVYTEDEEAEAADVEEPALVSDKEED"
     gene            2193..4835
                     /locus_tag="PLF14_05875"
     CDS             2193..4835
                     /locus_tag="PLF14_05875"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOT70858.1"
                     /translation="MKTNTLCKLVLLLITLSILLCSCESATPTVSYVPSPQAEQSEQP
                     VNTEVIPSPTDTPTPTPTPTSTPLPAKPTAYTDGKYFTVDELNGVILDYNTEGGVYVN
                     IPETINGVKIRGIGWQAFSFKPLYGVILPDTVTYISDQAFSNTGLVEVHLGNNVEHIG
                     FRAFSDNVKINIKYKDGGRDWNFLSNGIEQIKLPDKLKYIGDKAFDKNSITDLKLPEG
                     LEYIGKNAFANNNLSSADIPDSVTYIGKDAFLGLDLNKIKTGKGHPSHLSIDDDTITG
                     AIGKPMSTLVIPSIPGVNKINPLSFNDYEGITEVDIKPGIEYIGRYAFRTINRLDPQR
                     FVTLPDGIKYINQTAFWPVEKHIFNNAVTTQPGIPDFSDKSPANPLYSFPQSRTEIIR
                     TNISQEEISTFDEISPLEEWAQRYICTKYKDKDITSCNAYCNSSWRDEYYLADVLMCD
                     DYNLIAKMCTITLRTNIDIEGLTKKDLDYYISVQIVYTEDKETGQVTLCGFLDKNEAL
                     ETKKDLYDYIKADGRFKLNANKLSKKLSQIQSAIHMEDLSKCPVITELTRDVEYFQGS
                     LMAHEHYLPAGKDCVIFFVKDPVNKLFKVNYKTNEIIWEKGYGLIDSGILKYTDRFPD
                     EVNGVFKFMYYNGEYFTDFIDTDGKVIVEDAKDYRVDYISGTDWKVISDNGKITLEDR
                     KTGKYYMILDHVGFSGMNFTSSRFVKAISKNKIVIESTFPGEGAYHNWYFSYDIDTGR
                     LTHMTVYDDLLISNKDDKYVSKIIGTYEYYNKEILYIQNPDDNTSIDIRNWTDPFVCI
                     NAYALSKDGKYLALLTADSLIKDEYVITYGNTGIIIIDLEQKKVLVQDELILSYGSLS
                     FLDDNTLVVNDQKTMIYIDVPSVK"
     gene            complement(4828..5697)
                     /locus_tag="PLF14_05880"
     CDS             complement(4828..5697)
                     /locus_tag="PLF14_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT70859.1"
                     /translation="MRQLLILSGKGGTGKTTIASAFIKLADAKACADCDVDAPNLHLV
                     TKWKTQPQIKDYYGLPKAEINPDLCILCGQCKEHCRFDAILLSDGEYKMKPFACEGCG
                     VCELVCPAQAITMIPSVDGELTLYSDNEKVFSTAQLKMGSGNSGLLVTEVKKQMKSVE
                     VNTDFAIIDGSPGIGCPVIASLSGVDMVLIVAEPSISGISDMERIIGTAAIFGIEIAV
                     CINKYDTNIGNTEKIEDYCKKHGIALVGNIPFDPAAVTAINNGQTVVDIDCISGTAVK
                     DVYNRTMALLLAI"
     gene            complement(5669..6535)
                     /locus_tag="PLF14_05885"
     CDS             complement(5669..6535)
                     /locus_tag="PLF14_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT70860.1"
                     /translation="MRIAVLSGKGGTGKTLVSVNLAAVAKSATYIDCDVEEPNGHLFF
                     APEGIKEEDVTVKVPKVDETLCNGCRKCVTFCKFNALAHTADKLIIFDDICHFCGGCM
                     LVCPQNALTETDKVIGRIQKGTSEHVSVYSGILNVGVASGVPIINQLLEEKNFAANKL
                     TVIDCPPGASCVVMESIKDVDYCILVAEPTLFGVHNLELVYQLVKLFKKPFGAVLNKC
                     LEGENPAERFCIENNIKILGKIPFDTDLGALNSDAKIVAYEEKKYKDMFSSLLDRVVK
                     EVKHATVTDIKR"
     gene            complement(6546..6905)
                     /locus_tag="PLF14_05890"
     CDS             complement(6546..6905)
                     /locus_tag="PLF14_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014825362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOT70861.1"
                     /translation="MKVAVPVTEKSYEANVYASFGRAPYFLIYDTETKESKFIENSAA
                     TSMGGAGIKAAQTIIDNKVKVVLAPRCGQNAAHVIQSADILIYKTVAGSAKDNIDAFV
                     AGKLSLLDEIHAGFHRR"
     gene            complement(6939..7298)
                     /locus_tag="PLF14_05895"
     CDS             complement(6939..7298)
                     /locus_tag="PLF14_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOT70862.1"
                     /translation="MARPMKRRRVCYLPKIKRFGPLDLPTGANGFVNMTVDEYETIRL
                     IDLEGLMQEECAKQMNVARSTVQGIYVEARKKLAESLVNGKVLLIDGGEYLLCDEFVN
                     DCGRRCNRHRRGRRFEE"
     gene            complement(7407..8369)
                     /locus_tag="PLF14_05900"
     CDS             complement(7407..8369)
                     /locus_tag="PLF14_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HOT70863.1"
                     /translation="MNCLCCGKPLVEKNNASFWHDACVKKFFFTKKFPSMDLSDEILE
                     RYVNENVKKGISVAGVQKKISLQLTNDNNLRLTLVNYPTGYILKPQVSEYSSLPEAEY
                     LVMRMAFETGIKTVPFALLMMEDNSYAFITRRVDRILISKRSLKTHMLAMEDFCQLSG
                     RLTADKYHGSYEQCAKIISRYSINSGLDLSELFFRIVFSYAVGNSDMHLKNFSLIETA
                     PGSRQYVLSSAYDLLPVNIILPEDEDQFALTLNGKKRNIRRKDFLTFAENSQIPIKSA
                     EKIINQVVSLKDTYLSLCRDSYLPDNMKSELEQLITARISVLDS"
     gene            complement(8366..8698)
                     /locus_tag="PLF14_05905"
     CDS             complement(8366..8698)
                     /locus_tag="PLF14_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HOT70864.1"
                     /translation="MQDSYRTVFVYVQDIYAGVLKETDTGYLFQYDKEYLNNPKAAFV
                     SLTLPKREEAYQSKTLLSFFDGLIPEGWFLEVVSRNWKINKQDRFGILMVACQDCIGD
                     VSIRQVKK"
     gene            complement(8698..8898)
                     /locus_tag="PLF14_05910"
     CDS             complement(8698..8898)
                     /locus_tag="PLF14_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015534972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Y4mF family
                     antitoxin"
                     /protein_id="HOT70865.1"
                     /translation="MDTIGKYIKRKRKEAGLTQVDFAIRSGLGLRFIRELEQGKETVR
                     LDKVNQALGMFGMKAVPGRKDE"
     gene            complement(9016..10416)
                     /locus_tag="PLF14_05915"
     CDS             complement(9016..10416)
                     /locus_tag="PLF14_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006061085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT70866.1"
                     /translation="MQESQTTEWKERWKDEYLAWICGFANSTGGIIVLGRSDAGEAVG
                     VINAKKLMTDLPNKIRDALGIIVEIKLVQEGGKDLIEIIVPSYPVPISCNGSYYVRSG
                     ATNQRLSGSALESYILSKRGVNWDSLPIPGFKMEDINDKAVDHFKKLAAKKGRIPAEY
                     LDEPNDVLFSRMGLIKNGYLTNAAMLLFSENPQRWQQGAYVKIGFFRNHADLEYQDEI
                     HGPLINQIDRVMEVVHLKYMKARITYEGIQRIERYFVPDEALREAILNAICHKDYSSK
                     IPIQISVYSDQLYIGNVGRLPEHWTLDDLLGKHNSIPFNPDVANVFYLAGHIESWGRG
                     IEKIFNACKVENVPMPQFKVHPGDIMIHFTAPYGFSELGVKYDIGSKQNDQDSDQVSD
                     QGIKVKDILAFCTVERTLESIMKWFGMSHRTYFRRTYINPLLEEGLLRMTIPDKPTSK
                     NQQYITTQKGRELSEK"
     gene            10816..12867
                     /gene="ftsH"
                     /locus_tag="PLF14_05920"
     CDS             10816..12867
                     /gene="ftsH"
                     /locus_tag="PLF14_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOT70867.1"
                     /translation="MSSNNEDRINNVSETEQDDIRSNGPNQINSNDSNNRYSGNTGRD
                     GGPASNVPRGPNIFNIILIIFLITFIINTLFAGRLSKSVQVIQYSQFMELVESGNVSQ
                     VLLSDEQLQITISNDADLAEVEKIIYPTGRTEKQPAPASGTNYIAVRMEDSDLIHRLA
                     DNGVAFSQVISNTKVSPLRSFITMWIIPTLIMYLIYFFVIRNVIKKIGKKAPGGGMGG
                     IFGVGKSKAKEYNIEKNTGVTFDDVAGQDEAKESLKEMVDFLHNPEKYREIGAKQPKG
                     ALLVGPPGTGKTLLAKAVAGESGVPFYSISGSEFVEMFVGVGASRVRDLFENAKKNTP
                     CIIFIDEIDAIGKRRDNQLGSNDEREQTLNQLLAEMDGFDSGKGIVVLAATNRPEVLD
                     KALLRPGRFDRRIIVDKPDLIGREAILKVHAAKVKLDSDVNLREIALATSGATGADLA
                     NMINEGALLAVKEGRQKVSQKDLMEAVEVVIAGKEKKDRVMNPKEREMVAYHEVGHAL
                     VSALQKNSQPVQKITIVPRTMGSLGYTMSMPEEERYLMTESEILAQITTLLGGRAAEN
                     LIFGEVSTGASNDIERATKLARGMITQYGMSDKFGPMGLESTQNQYLDGRNVAHHSEQ
                     TGALIDKEVSSILVQCQQNAFNLLQDNRDALERISKYLLEKENITGKEFMQLLEETRS
                     K"
     gene            complement(13045..13986)
                     /locus_tag="PLF14_05925"
     CDS             complement(13045..13986)
                     /locus_tag="PLF14_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70868.1"
                     /translation="MEIREIEKIINEKISPYSLNEEGISIVSKLVNNFSFELLKECID
                     TGVNAYFRFDKEGNLTEESVDTFMKKLGGIAYNRLKTPLDKQIFSIKNRCWHKFPDWN
                     TDKANDILIRYISALKSKGLTEELIINDLQTDVSKMCSQSSDWSDWSSGMEYWIDNIK
                     DWPDEDSVAIKHNESIITNDVIEGFLHNSPNMQSLCKQINASYENNLYDCTAVIMRRL
                     LENLLILTYENKELEDDIMDESGRHYLPLSDIIKNAEKNPKLHLSSNVRKDMSLFKDL
                     GNDSAHKIRYNSTKQDIEPHIIKYRTIVRELIKKAGL"
     gene            complement(14104..14180)
                     /locus_tag="PLF14_05930"
     tRNA            complement(14104..14180)
                     /locus_tag="PLF14_05930"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14144..14146),aa:Met,seq:cat)
     gene            complement(14187..14274)
                     /locus_tag="PLF14_05935"
     tRNA            complement(14187..14274)
                     /locus_tag="PLF14_05935"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14238..14240),aa:Leu,seq:taa)
     gene            complement(14332..15414)
                     /locus_tag="PLF14_05940"
     CDS             complement(14332..15414)
                     /locus_tag="PLF14_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70869.1"
                     /translation="MKKVCLLIICLFILSFILTGCDNQPWINMVDTLMQGDYRKANKQ
                     YEKIEKNYVYAMNTSEYVYQYIENIVTSFKQDKTSYETAEKKLNLFSLLEMPLYNLED
                     AKSEVERIQASRTAYNQARELYLAELYYDAHNKFKLVEIDDSRNYDKAQEYLLSIVYV
                     LLYREEYYKAADVILSVDINSNTVPKLAEYSVDLAIRLLYYKDDMDRAYELNDKLIKL
                     LIRMEPGVKDAIKEWSRKLESIYSRPEYAAVCISFLSDDMEDMFYKLEQTDLRLETQK
                     TDYAFIILKSIPVRQDTIGYIAPRMTEILYQYISQGKPDYAQSVVDIIFSEIDDDRMV
                     KSLVTYIQDNIKNKEYLNKIDMTVGR"
     gene            complement(15459..16169)
                     /locus_tag="PLF14_05945"
     CDS             complement(15459..16169)
                     /locus_tag="PLF14_05945"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOT70870.1"
                     /translation="MTNISIWGDSVLRGVVYDDNAGKYVFLQNSCIPETAKNFNITYV
                     NNSKYGMTAPKALERMKKNLEENDIKPDFALIELGGNDCDFNWKEVASNPDINHDPNT
                     PIKVFDKTIRQMVTMFREKGIEPVLTNLHPIDPVRYLDWITKDGTSKEAILKWLKDVF
                     KIYRFQELYSLTIEHISKQLNVRLIDIRKAFLDAKNLMELLCRDGIHPNEKGHEIIAS
                     CLIEYISDYKREVLQGAV"
     gene            complement(16278..16955)
                     /locus_tag="PLF14_05950"
     CDS             complement(16278..16955)
                     /locus_tag="PLF14_05950"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOT70871.1"
                     /translation="MMNVADYILILIIIVSMILGGIRGIIMSAYGLLTTILSIFLSFK
                     FAQPIRDLLVGTSFYNNIHEKIEHMAEALVPNLDSVEEAIIATVNSLPFPDDIKIWLI
                     ERIDFSQTTTKAEAMGGLVANITDLIIAIIIGIILFVLLLIIFKLLRGLVKKISELPV
                     IKQVDAIVGTIIGAVSGVLLIYIIFLIISGFQTAEIMSVVHETIQKSKLASVLYNNNL
                     LLELFKK"
     gene            complement(17014..18120)
                     /locus_tag="PLF14_05955"
     CDS             complement(17014..18120)
                     /locus_tag="PLF14_05955"
                     /inference="COORDINATES: protein motif:HMM:NF039834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5711 family protein"
                     /protein_id="HOT70872.1"
                     /translation="MAKKRKKKAYWIIIVVLLAVSVSVGFIFFRTEILRFFKADVVAK
                     DAGVQYTIPVVTGSVVASENGYIYICTDNNLSIFKSNGEFLRSYLLSYTDTKIAYSDT
                     HVVVYDKGATSYTVFCEGKKVDEYTAKTAITYVFAFDEYMFLLCEGLQGYLGAVYCSI
                     YQTNLIIPDGYKNDIQYSERYPVYLDMFDDKEQFAVVSCVPDDMSKTHIEVFEIGKPT
                     PIAGITLDGFLPKTVCLQDGRFLVANDTDVLLIDKVMEKTSVFKDQQIVYIKGYENQA
                     YGYGIIDGEDRIFKIGSDGTLAWKKALQEDVQGFSVFENKLVIWKAQTLSMFTLKGND
                     FHSVETDSLIQQVIAIGSHRLAILTSRNVIVYKY"
     gene            complement(18189..19853)
                     /locus_tag="PLF14_05960"
     CDS             complement(18189..19853)
                     /locus_tag="PLF14_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007487875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOT70873.1"
                     /translation="MKTVLRITVLLFIVLSLVMGFATPVTALSKEELEKSVYAAAEYM
                     VNTVKNPQVGSIGGEWAVIGLARSGYEVPDGYYENYYATVENYVKACKGVLHEKKYTE
                     YSRIIVALSSIGKDARDVAGYDLTYALGDYDKTIWQGLNGPIWALIALDTKDYPIPEN
                     KEAKTQATRQKYIDRILKCQLKDGGWSLFGGSSIATSQDEISDPDITGMALQALAKYQ
                     DQPKVAKATKEALACMSGKQGSSGNFASWGTTNSESCVQMLVALCELGIPLDDSRFAK
                     NGVTLLDNLKTYQLEDGSFLHTYSGSGSNQMASEQGLYGVVAALRAIEGKNSLYRMSD
                     AVTVESSIDITVKKGEGLEGKHKDVLPMPITAPGTTFEDITFSPNIAAIEALASRGII
                     NGRSKKSFAPDANMTRAEFATIITRGLGLVEEDTKVFSDVSSDKWYAGYIGTANKYGI
                     VNGVGEGKFNPEGTITRQEAATMVARAAALCGLDTKMSNAEIRDVLSQFEDYVTVSDW
                     AMESMAFCYKKGILGDSALKVEPKVQIQRSEIAQMLFNMLTVANLL"
     gene            complement(19991..25636)
                     /locus_tag="PLF14_05965"
     CDS             complement(19991..25636)
                     /locus_tag="PLF14_05965"
                     /inference="COORDINATES: protein motif:HMM:NF025833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOT70874.1"
                     /translation="MQRSIKVNKFLAVILSLLILLQICPLGVFSATAIYELKDTTATK
                     IGSFTQSGQTMDIYKLSLDGSKYGSVQLKGRDMLGNRMPVLRTPSGQYKMIQLVRLGI
                     VSETNIGKECYENTKGFLPEDIVNDCMEENTIGYYWYGMQPMPGLGPAVAHIAGFIII
                     EWSSATAVNEKRETSINALTEVWEAAKETDAYKNASGETADIASNVDSTYNETVTAIN
                     MAPSIKEIEEVKNTGIEAINTIIAGGEATGPTVEYIITYITVEKLFIDGNFIVEPTEM
                     KLPKGLNAADLMMQALKARYPEKGEEAYRYNGGYLAGVWDPDFVYKGSGTVQKEGYLS
                     EKDAGSFSGWMYCVNERFPSTGMDGYTLEDNDVMRLIYVGHYSATGGGANRSDLIRKV
                     ANIRKQYSNSIEAITEIYGTAYDDAMTVLKQTTVGEYEVSSALNALITIETSVSVTDI
                     VDVPAEVAAETPLSLVGTVEPDNASHKEITWTVKNQGTTGAFIEKNILHTLSEGNVTV
                     TATIENGKIKEDFSKDFDITVTISLAQQLEEAKQARIDQINAVYTGVDETDYTKDSWT
                     RFYALIQSAIANVHAATTVDAINAVAIPSTDGLETVAAHLATLKTARIEKINAVINGL
                     TEADYTPDSWTELQTAITDAIQAVTDATDAAAVEAVNIPSISGLVAAGSELEEVKRAK
                     IAQINGVTNGFSETDYTADSWAALQTAIIDAIQAVNNATDVPAVKEVAIPSTDGLVAV
                     ASQLASAKEAKIAQIAAVTDGLNQSIYTKASWSALQSAMATATADVQNAKTLAAVEAI
                     SIPSTENLVTILDSEKAARIARFEAVFDGLVQAEYTEASWAALQTAVNNAKEEVNSAE
                     SVEEVKAVRIPSTDGLVTLKEELAAAKAEKKAQIEERNRYLYQLPYTPGSWTAYCRGY
                     QTTMASIDAATTVEEVNAITFPSESMLVLRGDKGALREAITLAETKNQDHYIEGWDEF
                     FNALRAAKQVEGDWNAAQGKVDNALANLNDRMTRLVLKDGYAGALNDVLNYIRSNVET
                     PELGSIGGEWAILSLARGGFSDTPYYDGYYSRILTSIADKPDKLNDNKSTENSRLILA
                     LTSLGIGAVNVNGKNLIAPLLSDSEWVKRQGINGAVFALIAMDSLPYAKNSPVRQELV
                     DYILSCQVGDGWSLDNTNPNLDITAMTIQALAPYKEQDDVSAAVGKALAWIVRQKITD
                     SEGCSQVIVALSALGEDAADFNGKNYISILLSYYDATNKAFLRESAANLMSSEQGAYA
                     LVAYDRFKNGKARLYDMSDVVKMVSDDVLPAVADKTALEEKIAQAERYSESDYTAGSW
                     SALQIELRDAKAVRDLETVTQAVVDNAVSSLVSAIAKLKIAEPAVQLKYVRLSVTDPN
                     AKANQQSVYYPPTAMEMNEGETAYSILRRTGLTLRVSDHSQYAGVYVEAISGFGEFDD
                     GPQSGWMYKVNGVFPGYSSSLYELKENDVVEWVYTRDLGKDVGGYLPGVEDTDTAEQG
                     VATLTPKADVTGSDATASVTDEQINSAVKAVKSGSSGSVSIAPSGADNVKNVAVNIPT
                     TAAKSIADEKNLSLEIDTPVGTVTIPNDALTSIANEAEGSDIEVSIAKKSAADVTDDS
                     IDTSSGIVISVSISSDGKEISSFGGKSITIDIPVSNEFEAGETYSVVHISKNGSTENI
                     KGECIKIDGKLYVRISVTHLSTFVVLLDDVVLAFTDLNKHWAVKEIKYVYAKGIMKGT
                     SETTFEPRETLSRAMLVTMLYRLEKEPKADSKNIFTDVKNGMWYTDAICWAASNGIVN
                     GYGEGIFAPNDSITREQMATILYRYAQYKNYDVSKTGDLTVFGDKKEISKWALGALKW
                     ANGEKLINGRTRTGIVPAGNTTRAEAAVMLTRFMKNVVK"
     regulatory      complement(25973..26163)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(26281..27303)
                     /locus_tag="PLF14_05970"
     CDS             complement(26281..27303)
                     /locus_tag="PLF14_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HOT70875.1"
                     /translation="MYQKLLGELKERVRSSQLKAAVKVNYELLDLYWNFGKEIALRQE
                     QCAWGDAFIKSLSKDLCKEFPKLQGFSETNLKYMKRFYLFYQKSQQAVDQLEYIFSIP
                     WGHHILLMTKCKSPEEALFYIEKTIKNGWSRAVLLNYLDTTLYTTQGKAITNFTTLLP
                     DSQSDLAKETLKDPYNFDFLTLTEGYREKELEDALTTNITQFLLELGQGFAFVGRQVP
                     IMVGSKEMFIDLLFYHLELRCYVVVELKATEFEPGYIGQLGVYVAAMNHQRKKEADNE
                     TLGLLICKTKDNVVAEYALESSSQPIGISEYHLSQLLPKDYRSSLPSIKEIEQGVMAI
                     GKEVFL"
     gene            complement(27474..30224)
                     /locus_tag="PLF14_05975"
     CDS             complement(27474..30224)
                     /locus_tag="PLF14_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HOT70876.1"
                     /translation="MESYTIKNLTFSYPEKEKKVLNNLSFSIERGQFVTLCGPSGCGK
                     TTLLRQLKTVLAPHGSLSGDIIFEGKPLSEVDIRTQSSKIGFVMQSPDNQIVTDKVWH
                     ELAFGLESLGFNTPAIRLKVAEMASFFGIETWFYKNVTELSGGQKQLLNLAAIMAMQP
                     SVLILDEPTSQLDPIAATEFISAVEKINRELGVTVIITEHRLEEVFAISDRVLVMDEG
                     RIICDGTPNEVGEQLKDLHHGMFLAMPVPMRVYAGVENSLACPVTVREGRTWLDTFTK
                     NKQLRDIPAEDSIKEQEGIPVIEFDEVWFKYEKDAPDVLKGLSMKIYQGEFYAILGGN
                     GTGKTTALSLISGINKPYRGTVRINGKERSKIGDKELFNRMLGVLPQNPQALFVKKTV
                     QEDLLEMLSGQKKSKAEKEQQIAKIAKLCRLEELLSMHPYDLSGGEQQRAALAKVLLL
                     EPKILLLDEPTKGMDAEFKRIFANILYELLYKGVTVLMVSHDIEFCAKYAHKCALFFD
                     GNIVSWNTPRSFFSDNSFYTTAANRMARHMLPTAITAEDIIYACGGKAEPFEIKIPED
                     RNIMPIDTKEDTAEEYDKSVLKSEKKNNKLPAWRIAGAVLTALAFLVTAYFAVSNMGD
                     LSAIIKQGDLSVVSFGKEMNIWHYVLLAVALAAELLLFFILLRPKHTKIDNEQVAADK
                     RKLSKRTLLAAAMILIAIPLTIYLGVYYLEDRKYYFISMLIILETMLPFVLIFEGRKP
                     QARELIIIAVLCAIGVAGRTAFFMLPQFKPVAAIVIIAGVAFGGETGFLVGAVTAFVS
                     NMFFGQGPWTPWQMFAFGIIGFLAGILFRKGILRRNTIALCIFGGLATFLIYGGIMNP
                     ASVLMYEAKPTMEMFYVAYIRGIPFDLIHAVATVVFLFSIAKPMLEKLDRVKLKYGLI
                     ER"
     gene            complement(30197..31087)
                     /locus_tag="PLF14_05980"
     CDS             complement(30197..31087)
                     /locus_tag="PLF14_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018758254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOT70877.1"
                     /translation="MKDTFSTYHPAVNFLYFTLILVFSMFFMHPVCLATSLLSALCYS
                     IYLNGKKALFFNLKYMLPLLIITALINPAFNHEGGTILTYLRDGNPLTLESITYGIAA
                     AVMLITVISWFSCYNAVMTSDKFVYLFGRIIPALSLILSMALRFVPHFKAQIKVVSDA
                     QKCIGRDVSNGSILQRAKHGIRILSIMVTWALENAIETADSMKSRGYGLPGRTAFSIY
                     RFDKRDKKAMLFLSLCGIYIILGAFLGGIYWRYFPTMKGVGFELYPISLYICYFALCI
                     MPVVINVNEDRKWKAIQSKI"
     gene            complement(31087..31821)
                     /locus_tag="PLF14_05985"
     CDS             complement(31087..31821)
                     /locus_tag="PLF14_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007493753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HOT70878.1"
                     /translation="MQVKGIKKHKGKIIAAAVILCVLVVTYIWGGNYAVSDISYNFAS
                     PSPTPCQREGSSEGVFEGSKSYSEQLGMVFDKETGKDRYLTDPVPEGKPIPVEPQDAV
                     ITDTAHTCILSVRCDTILNNMKYLDKEKVELVPKDGVIFPATEVTFYEGESVFNILRR
                     EMKKAGIHMEFVNTPMYNSVYIEGINNLYEFDVGELSGWMYKVNGWFPNYGASRYQLK
                     AGDVVEWVYTCNLGRDVGGYYAMGEE"
     regulatory      complement(31929..32141)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            32395..32471
                     /locus_tag="PLF14_05990"
     tRNA            32395..32471
                     /locus_tag="PLF14_05990"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32429..32431,aa:Asp,seq:gtc)
     gene            32475..32550
                     /locus_tag="PLF14_05995"
     tRNA            32475..32550
                     /locus_tag="PLF14_05995"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32508..32510,aa:Phe,seq:gaa)
     gene            32556..32630
                     /locus_tag="PLF14_06000"
     tRNA            32556..32630
                     /locus_tag="PLF14_06000"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32588..32590,aa:Gly,seq:gcc)
     gene            32640..32714
                     /locus_tag="PLF14_06005"
     tRNA            32640..32714
                     /locus_tag="PLF14_06005"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32672..32674,aa:Cys,seq:gca)
     gene            complement(32807..33571)
                     /locus_tag="PLF14_06010"
     CDS             complement(32807..33571)
                     /locus_tag="PLF14_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004825810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOT70879.1"
                     /translation="MIRGIESFRKWFQGCEEQYVIIGGTACDLLMTNEGLDFRATKDI
                     DLVLVIEALDVSFGKRFWEYIVAAGYEHRNKSTEAYQFYRFSKPQLQEYPAMIELFTR
                     KPDTIILPRNAILTPLPLDENISSLSAILLDDDYYNFLMQGRTKVAGVTVLDVTHLIA
                     FKAKAWLELSERNVACGDVDSRDIKKHKNDVFRLTELLYPDQEPLRNVPLTVLKDMRT
                     FLEKMETEDVDLIKLRIPNKTKDTILEELRSLYGLD"
     gene            complement(33555..34514)
                     /locus_tag="PLF14_06015"
     CDS             complement(33555..34514)
                     /locus_tag="PLF14_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008755226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOT70880.1"
                     /translation="MGYLNTLLGIHVVYKGDIVRNIPNFIHARYKLQKATLDGNEVIF
                     LFPKTELEPVSAIKKHIERIQKEENLHVILVPKKLTYRQKEYLLRDRIPFIVEEKQIY
                     LPFMGTYLQERSDGEKQMTDVMLPSAQLLFLYYIYNGCGELLTSEAAKALELTPTSIS
                     RASIQLEGMGLIKTEKRGVQKVVFSDKTVQELFTEGQKHLANPVKRRVYVPKSEIKER
                     LLFSGYSALSNYSMLNPSDIDFYATDSIARWEKIASKKLLNKDEQCAVEFWSYNPRKL
                     SKDENVDRLSLALSLREDKDERVEEAVQEMLEQVWRDIDDKRN"
     gene            complement(34837..35697)
                     /locus_tag="PLF14_06020"
     CDS             complement(34837..35697)
                     /locus_tag="PLF14_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70881.1"
                     /translation="MKKTKLLYIVCCIVFISLLVACQINKEKQPEASDSTEFRTVEDT
                     QTVVFVDYNWVVLKKEDAYTKNTVILTGEVSNVRYTTVDYEFMDTNVSDKATLFDIKV
                     DKVLSCTSDLYPDRDCITVGVGYTMSTYNEELPVITEGARYLLFCYVTADDKDDVRET
                     YKYADCWICYPKDLLCESIDGYYLVCDFFSDIPSAVSLAGAIGVTKEDASDIFSHVSK
                     EYIKRKMEASSKRKVISEEVALSCIETLKVRAGNGADQLWNIMKNRYLIEETAFENHI
                     RKTAESFSDR"
     gene            complement(35724..>36481)
                     /locus_tag="PLF14_06025"
     CDS             complement(35724..>36481)
                     /locus_tag="PLF14_06025"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOT70882.1"
                     /translation="LRIINRDNPITQDEANDTTGLIQLKDKNYVLDNRDDLFILPHVK
                     QALEELLAAADKQDASLKMHVASAHRLYESQKQLFDYWVNRRVTQENMTYAQAYEYTA
                     GRVAIPGCSEHHNGLTLDVVGYGYMMDESSKGAPYAIWLKNNSYKYGFTVRYEEGKEQ
                     YTKITLNEPWHLRYVGLPTAFYLYNENLCMEEFYQKLLTEKLIDFTYDNETYRHIYAK
                     NDNIYIDENVMETVQYSRIASNIEGYVVLCKVK"
BASE COUNT        10357 a         8217 c         6473 g        11434 t
ORIGIN      
        1 ccagtcaaaa gcatcctgcc tgtatttttc tgcaaattcc ctgtgggctt gttctccgcg
       61 gaaccttgca aggctttctc tgtactgctg aataccgccg cccacataca tggttctccc
      121 cattacctgt gatgcgactc tcgacgaaac ggagccgcaa tagataggta catgatcgct
      181 tttttcttta gaaaatgccg ccattactct ttcatacgaa ttcataatgc tttatatata
      241 cagtcagcaa ctgttcctcc ctctctttct ttttaaataa aatgacaccg atacaaattg
      301 attatatccg tcaacagcgg tcgtttaata tcattcagtc tttgtcgtgt atatactccc
      361 atatattatt gccggtcttc aaatatgtgc ccgctccgta aagttgatac tgttcgtaaa
      421 aggaaatacc gtctacggaa ttgggcttaa tttctacacc ggcacctatt gtaatagtgc
      481 gcagtttgcc gtttcctaga aatacattct ctccgagata tataagattg gcgggcagag
      541 ttacactttt catatcgcta ctctccgcaa aagcataatc ttgtatatat gttacagtat
      601 ccggtatatg aaccgtgtaa agcgggcttt tgtaaaaagc cttttctcct attattcttg
      661 ttccgtgtgc tatatagtat tccgttctca taaacgaaac ggggaaatac actatttccg
      721 acatgccgtc cttgaatagt acaccgttaa atgacttata tattctgtta ccctgatcca
      781 cctctatttt cgcaaattcg ggacactcac taaaagccgt cggacttata taagtaaccg
      841 atttagggat aaatacttct cttaaagcgg aacagttata gaaagattca tcctggagtt
      901 ctataatgcc gtcatccaat ttaacggtta tcatggagga gttatcgcaa aaagctctgt
      961 ttcctattga ggcaaccgct ttgttatcta ttgatgccgg tataacgggg tgtttggcat
     1021 ttgtcttgca ggaaataatt gtttctcccg atacgacata ctcaactttt gtttcataag
     1081 taataataac atacatcagt acggctgcgc ataatataaa cactattaga gatatcggtt
     1141 tgaggtactt catttaaaac cctttctacc gttagattat aacataaacg aacataaaaa
     1201 aaggggctgt tagaaatttc ttaacagctc ctttaaaaca tgatcaatta attgttcagt
     1261 cctcttcctt atcggataca agagcaggct cctcaacatc cgcagcttca gcttcctcat
     1321 cttccgtata taccttaggt accgttacgg taacaacaac gctgtccggc ggtgtaacca
     1381 attctatttt ttcattcttt gctatatcaa gttcctccac cgtcaacaca tcaccgggtt
     1441 tcatttgcga aacatccact tccacacggt caacaaaatc tgcaggaagg gcgctgtatg
     1501 ttatttcatc cagcatatga atgagcacac cttctcttat tctttctctt ccgctgatta
     1561 tgactctggc tacgcttctt gtaacctccc cggctaccaa ggcctgaaag cttaaatgct
     1621 ccagtttatt ggcaccggga gtgtatgtgg cttccttcag taccgccata tacttttctt
     1681 tacctatcac tatttccact tttgaaccga tgaaattgta ttgcagaaat ctctcggctt
     1741 catgtacggg aagctgtaag caaaccgatt cctccagatg ctttccgtaa agcaccgccg
     1801 gtattatgcc tttatttcta agttgtcttc cttttaaatt cttttcacgt ttttcaaagt
     1861 ttaatgtaac cacgatatct cctttcaagg tcagttatca ttactgccga atattcagcc
     1921 taaaagatat tttaccacga ttttacaggc aaggcaaata gaaatgtaga gagagattct
     1981 gatatgttct gatatcaatt tcgttctgta tatttaagaa aatatctcgg tatttcactt
     2041 atcttgggtt aagtacacct gtgcggcgtg tacatgtcga gcggaagcgg taaagaattc
     2101 atgtaatgtt cttaatttat tcacagttta ttaatatatt ctgtttatat tataattgtt
     2161 atatcatcat taatacttac ttcgaggtag acatgaaaac aaatacttta tgcaaattag
     2221 ttcttttact gataacatta tccattcttt tatgttcctg tgagagtgca actcccaccg
     2281 tatcctatgt tccctctccc caagcggaac aatccgaaca acctgttaat acggaagtta
     2341 taccttcacc gaccgatact ccaactccaa cacctacacc tacttccaca ccgttgcccg
     2401 caaagcctac agcatatacc gacggtaagt atttcaccgt ggatgaactg aacggagtta
     2461 tattggacta caatacggaa ggcggagtat atgttaatat tcccgaaacc ataaacggtg
     2521 taaagattag aggcataggt tggcaggcct tttcttttaa acctctatac ggagtcatat
     2581 tgcccgatac ggtaacatat atcagcgatc aggcattttc caataccggt ttagttgagg
     2641 tgcatttggg taacaatgtt gaacacatag gtttcagggc tttcagtgat aatgtgaaga
     2701 taaatataaa gtataaagac ggtggccgtg attggaattt cttatctaac ggcatagaac
     2761 aaattaaact gcccgataaa ttgaaataca taggagataa agcttttgat aaaaactcca
     2821 ttaccgacct aaaactgcct gaaggattgg aatatatagg taaaaatgct tttgcgaata
     2881 ataatctgtc ttcggcggat atacccgaca gtgtaactta tataggcaaa gatgctttcc
     2941 tgggacttga tttgaataag ataaagacag gtaaagggca tccgtcacac ctttccatag
     3001 atgacgatac gataacggga gctataggaa aacctatgag taccctggtc ataccgagta
     3061 taccgggtgt caacaaaata aatccgttat ccttcaacga ttatgaagga ataaccgaag
     3121 tggatattaa gccgggaata gagtatatag gaaggtacgc tttccgcact atcaacaggc
     3181 tggacccgca aagatttgtc accttgccgg acggtattaa atatataaat caaactgcat
     3241 tctggccggt tgagaaacac atcttcaaca atgctgttac aacccaaccg ggtattccgg
     3301 acttttccga taaatcacct gccaatccct tgtacagctt ccctcagagc agaaccgaga
     3361 ttatccgaac aaacatatca caagaagaaa tttccacttt tgatgaaatc tctcctttgg
     3421 aagagtgggc tcaaagatat atatgcacaa agtataagga taaggacata acaagttgca
     3481 acgcatactg caacagcagc tggcgtgatg agtattactt agctgatgta cttatgtgtg
     3541 atgattacaa tcttattgcg aaaatgtgca caataacact ccggacaaac attgatatcg
     3601 aaggtttgac gaaaaaggat ctcgattact atatatccgt tcaaattgtc tatacggaag
     3661 ataaagaaac aggacaggtt acactatgcg gcttcctgga taaaaatgaa gcattggaaa
     3721 caaagaaaga cttatacgac tatataaaag cggacggaag atttaaactt aatgcaaaca
     3781 aactcagcaa gaagctttca caaatacagt cggcaattca tatggaagac ttatcaaaat
     3841 gtcccgttat aaccgaactt accagggatg ttgaatattt tcaaggcagt ctcatggcac
     3901 atgagcacta tttaccggca ggtaaagact gtgtaatatt ctttgttaaa gaccctgtaa
     3961 ataagctttt taaagtcaat tacaaaacaa atgagataat atgggaaaaa ggctacggct
     4021 tgattgattc gggaatactt aagtacacgg acagatttcc cgatgaggtg aatggcgtat
     4081 ttaagtttat gtattacaac ggtgaatatt ttacggattt tatagatact gacggtaaag
     4141 ttattgttga agatgcaaaa gattatcggg tggattatat atccggtacc gattggaagg
     4201 ttataagcga taacggaaag ataactcttg aggatagaaa aaccggaaaa tactatatga
     4261 ttcttgatca tgtcggattt tcaggtatga attttacttc aagcagattt gtaaaagcaa
     4321 tcagtaagaa taagattgtt atagaatcca catttccggg agaaggagca tatcataatt
     4381 ggtatttctc atacgatatc gatacaggtc gtctaaccca tatgacagta tatgacgacc
     4441 tgcttatatc caacaaagat gataaatatg taagcaaaat aatcggcact tacgagtatt
     4501 acaacaaaga aatattatac atacagaacc ctgatgacaa cacctctata gatatacgta
     4561 attggacgga tccctttgta tgtatcaatg cttatgcgtt atcaaaggac ggtaaatacc
     4621 ttgctcttct aactgcagac agccttataa aagacgagta tgttataacc tatggtaata
     4681 ccggcataat aataatagac cttgaacaga aaaaggtatt ggtgcaggat gaactaatat
     4741 taagttacgg aagtttatct tttcttgacg ataacacatt ggttgtaaac gatcaaaaaa
     4801 caatgatata tattgatgtg ccgtccgtta aatagcaaga agcagagcca ttgtcctgtt
     4861 gtaaacatcc ttaaccgctg ttcccgatat gcagtctatg tccacaacgg tctgaccgtt
     4921 gttgattgct gttaccgctg ccgggtcaaa gggtatattg cctacaagag ctatcccgtg
     4981 ctttttacaa taatcttcaa tcttttcggt gttaccgata tttgtatcgt atttattaat
     5041 acatacggct atctctattc cgaatatagc cgccgttccg ataatacgtt ccatatcgct
     5101 gataccggat atggaaggtt cggcaacaat aagaaccatg tccactccgc ttagagaagc
     5161 aattaccgga cagcctattc ccggtgagcc gtcaataatt gcaaaatccg tattcacttc
     5221 taccgacttc atttgttttt tcacttccgt gacaagtaac cccgagttgc cgctgcccat
     5281 tttgagctgt gcggtggaaa aaactttttc attatcggag taaagggtta attcaccgtc
     5341 cacagaagga atcattgtta tggcttgggc gggacaaacc agttcacata caccgcaacc
     5401 ttcacaagca aaaggcttca tcttgtattc tccgtccgac aacaggatag catcaaaacg
     5461 acaatgctct ttacactgac cgcataagat acacaggtcg ggatttatct cggctttggg
     5521 taatccgtaa tagtccttta tttgaggttg cgttttccat tttgttacca gatgcaaatt
     5581 gggggcatcc acatcgcagt cggcacatgc tttggcatcc gccaacttta taaacgcact
     5641 tgctatggtg gtctttccgg taccgccttt accgcttaat atcagtaact gtcgcatgtt
     5701 ttacctcctt taccacccta tcaagcaatg aagaaaacat atctttatac ttcttttctt
     5761 cataagcaac tatctttgca tcggagttta atgctccgag atcggtatca aacggaatct
     5821 ttcccaagat tttaatatta ttctctatac agaaccgctc tgcaggattt tctccttcta
     5881 agcatttatt aagcaccgct ccgaacggct ttttgaataa tttgaccaat tgatatacca
     5941 attccaggtt atgtacaccg aacaaggtag gctcggccac cagaatacag taatctacat
     6001 ctttgatact ttccattaca acgcaagaag ctccgggagg acagtcaata acggtcagct
     6061 tatttgctgc aaaattcttt tcctccagca gttgatttat gatgggaact cccgaagcta
     6121 caccgacatt caatatcccg gaatataccg atacgtgttc ggaagttcct ttctgaatcc
     6181 tgcctataac cttatctgtc tcggttaatg cattttgggg acaaaccaac atacaaccgc
     6241 cgcaaaaatg gcagatatca tcgaaaataa tcagtttatc cgcagtatgc gccaatgcat
     6301 tgaacttgca gaaggtaacg catttacggc atccgttgca taatgtttca tcaactttcg
     6361 gaacctttac cgttacatct tcttctttaa ttccttcggg cgcaaaaaac agatggccgt
     6421 tcggttcctc cacatcacag tctatatatg ttgcactctt tgcaaccgcc gctaagttga
     6481 ccgacaccaa tgtttttcct gtaccgccct taccgctcag tacggcaatt ctcatattat
     6541 tatcctcagc gtctgtgaaa tccggcatga atttcatcta ataacgaaag tttgcccgca
     6601 acaaaagcat caatgttatc ctttgccgaa cccgcaaccg ttttataaat caaaatatcg
     6661 gccgactgta taacatgagc tgcgttttgt ccgcaacgcg gagccaatac aacttttact
     6721 ttgttatcta ttattgtctg tgcagcctta attcccgcac ctcccatgct ggttgcggca
     6781 ctgttttcta taaatttact ctccttggtt tccgtgtcgt aaataaggaa atacggagct
     6841 ctgccgaatg atgcatatac attcgcctca taacttttct ctgttaccgg aactgctact
     6901 ttcatgataa tcctcctgtt tttttaatat tcaataacct attcttcaaa acgtctgccg
     6961 cgtctgtgtc tgttacatct acgtccgcag tcatttacaa attcatcaca aagcaggtat
     7021 tcaccgccat caatcagcag aacttttccg tttactaagg attcggctaa cttttttctg
     7081 gcttcaacat aaataccctg tacggtactt ctggctacat tcatttgctt tgcacactct
     7141 tcctgcatta atccttccaa gtctataagc cttatggtct catactcatc tacagtcatg
     7201 tttacaaaac cgtttgctcc ggtaggtaaa tcaagcggtc cgaatcgctt aatcttcggt
     7261 aagtaacaaa cccttcttct cttcataggt ctggccatta atgattacct cctctgcctg
     7321 cacaaatagt ctacacctat aattatatat tgtttccggc atatgtcaat aacttgtctg
     7381 tttgtatata gattgatata gaaaatttaa ctgtccaaaa cagatatcct tgcagttata
     7441 agctgctcca attccgattt catattatcc ggaagatacg aatcccgaca caaggaaaga
     7501 tatgtgtctt tcaatgacac cacttgattt attatctttt ccgccgattt tataggtatt
     7561 tgcgaattct ctgcaaatgt gaggaagtct ttccttctta tattccgttt cttcccgtta
     7621 agagtaagtg caaattgatc ttcatcttca ggaagaataa tattaaccgg caacaagtca
     7681 tatgccgaag agagaacata ttgtcgacta ccgggtgcag tctctattaa tgaaaaattt
     7741 ttcaaatgca tatcgctatt acctaccgca taggaaaaca ctatcctgaa gaaaagctct
     7801 gacagatcca atccgctgtt aatcgaatat cttgatatta tcttggcaca ttgctcatat
     7861 gatccgtgat atttgtccgc cgttaatcgt ccgcttaact gacagaagtc ttccattgca
     7921 agcatatgtg ttttaagact tctcttactt atcagaatac ggtcaacacg tcttgtaata
     7981 aatgcataac tgttatcttc catcattaat aatgcaaagg gaacggtttt aatacctgtc
     8041 tcaaatgcca tgcgcataac caggtattcc gcctccggta aagaagaata ttcagacacc
     8101 tgaggtttta aaatatatcc tgtcggatag ttcaccaaag tcaaccttag attgttatca
     8161 ttagtcaatt gaagagatat tttcttctga actccggcaa cggaaatccc tttttttaca
     8221 ttttcattta cataccgctc caatatttcg tcagataaat ccatactcgg aaattttttt
     8281 gtaaaaaaga attttttcac acaggcatca tgccagaatg aagcattatt cttttcaact
     8341 aaaggttttc cgcaacataa acaattcatt ttttcacctg tctgatgctc acatcaccaa
     8401 tacaatcctg acaagccacc atgagtatac cgaaccgatc ctgtttattt attttccaat
     8461 tgcgggatac aacttctaaa aaccatcctt cgggtataag cccgtcaaaa aaggacaaga
     8521 gtgttttaga ttgatatgct tcttcccgct tcggtaaagt caggcttaca aaagcagctt
     8581 ttggattgtt cagatactct ttatcgtatt gaaacaggta tcccgtatcg gtttctttca
     8641 atacaccggc atatatatcc tgcacataca caaaaactgt tctgtaggaa tcttgcatca
     8701 ttcatccttt cttccgggaa ccgctttcat accgaacatt ccgagagcct gatttacctt
     8761 atccagtctg actgtctctt ttccctgctc cagctcgcgt ataaaacgaa gtcccagtcc
     8821 ggaacgtata gcaaaatcca cctgcgtcaa gcctgcttct tttctcttcc gttttatata
     8881 ctttccaata gtatccataa ccacatatta cacccgatag ggtaatatgt caacactttt
     8941 gctaataatt atacctatat gggtataata tatattattt tcacatattt atacctttta
     9001 gggtatattt tcctattact tttccgacag ttcacgtccc ttttgtgttg ttatatattg
     9061 ctgattctta cttgtcggtt tgtccggaat tgtcattctc aataagcctt cttcaagtaa
     9121 cgggttgata tatgttcttc tgaaataggt tctatgagac attccgaacc atttcattat
     9181 gctttccagc gtccgttcaa ccgtacaaaa agcaagtatg tcttttactt taataccttg
     9241 gtcactgact tggtcactat cttggtcatt ttgcttactt ccgatatcat atttgactcc
     9301 taattcggaa aaaccgtaag gagcggtaaa atgaatcatt atgtcaccgg gatgtacctt
     9361 aaattgcggc ataggtacat tttccacttt gcaggcatta aaaatttttt caataccgcg
     9421 cccccagctt tcaatgtgac cggcaagata aaaaacattg gcaacatcgg gattgaaagg
     9481 aatggaattg tgtttaccca ataagtcatc aagtgtccaa tgctcaggga gcctgccgac
     9541 attacctatg taaagctgat cgctatacac actaatctgt atgggaatct tactgctata
     9601 atctttgtgg catatagcgt tcagtattgc ttctctcaag gcttcatccg gaacaaaata
     9661 gcgttctatt ctctgaatac cttcgtatgt aattctcgct ttcatatact taaggtgcac
     9721 aacttccata acacgatcta tctgattaat caaaggtcca tggatttcat cctgatactc
     9781 caaatctgcg tgattgcgaa agaaacctat cttcacatat gcaccctgtt gccatctttg
     9841 aggattctcg gaaaataaaa gcattgccgc attggtcaga taaccgtttt taatcaatcc
     9901 catccgactg aaaagaacat cattcggctc atctaagtac tcagcaggaa tcctgccttt
     9961 ttttgctgcc aattttttaa aatggtcaac tgctttatca ttgatgtctt ccattttaaa
    10021 accgggtata ggtaaactgt cccaatttac acctctttta cttaaaatat agctttcaag
    10081 tgcactcccg cttaagcgtt gattagtagc accgctacgg acataataac tgccgttaca
    10141 ggaaatcgga acaggatagg aaggtactat aatttcaatc aagtctttcc cgccttcctg
    10201 aacaagctta atctcaacaa taattcccaa ggcatcacga attttattcg gtagatcggt
    10261 catcaatttc ttcgcattaa tcacacctac agcttctcct gcatcgcttc tacccaatac
    10321 aataattccg ccggtactat tagcaaaacc gcaaatccaa gccaaatatt catctttcca
    10381 acgttctttc cattccgttg tttgtgattc ctgcaaggtc gtgtactcct tcatttattt
    10441 agtaactaaa catattttac catctgtatg caagcaattc tattaaaaac aacatatttt
    10501 aaccgtatct gaactatgtt acatatttta gaatatatcg caaatatgtt gcacaattct
    10561 aaagtcccgc gaatttgcaa aggagatttt tgcgtaaccc gttgtcggat atttttaaat
    10621 gtaaaaactt ttctgtaatt cgtaatatca tgtacatcaa ctaaatcatc taagcatatt
    10681 aaattccttc cgttattacg gtaaattcct attatattgt ttttttatga tatgattaag
    10741 tatataacat gctaccgttt tggagccttt tgaaaatgtt gggtacagcg tgtactcaag
    10801 gacgaggaga ataaaatgag ttctaataat gaagacagaa taaacaatgt ttcggaaacg
    10861 gaacaggatg atatccgaag taacgggccc aatcaaataa attcaaatga ttcaaataac
    10921 agatacagcg gcaacaccgg aagggatggc ggtcctgctt ccaacgtgcc cagaggtccg
    10981 aatattttca atataatatt gataatattt cttatcacct tcataataaa tacacttttt
    11041 gcaggcagac taagtaagtc ggttcaagtc attcaataca gccaattcat ggaattagtg
    11101 gaaagcggga atgtcagcca ggttttatta agtgacgagc agcttcagat aacaatcagt
    11161 aatgatgcgg atcttgctga ggtggaaaag attatttatc cgaccggacg caccgaaaag
    11221 caaccggctc cggcatcggg caccaattat attgcagttc ggatggaaga ttccgattta
    11281 atacatcggt tagccgataa cggtgtagcc ttcagtcaag ttatcagcaa caccaaagtt
    11341 tcaccgttaa gaagctttat caccatgtgg attatcccca cccttattat gtatctgatt
    11401 tacttctttg taatccgcaa tgttataaag aagataggaa aaaaggcacc cggcggcgga
    11461 atgggcggca tattcggagt aggaaaaagt aaagcaaaag aatacaatat tgagaagaat
    11521 acgggagtca catttgatga tgtagcggga caggacgagg ccaaagaaag cctgaaagaa
    11581 atggttgatt ttctgcataa tccggaaaaa tatcgtgaaa tcggtgcaaa acaacccaaa
    11641 ggagcccttt tggtgggacc tcccggaacg ggaaagacac ttctggcaaa agcggttgcg
    11701 ggagaatcgg gtgttccctt ctattccatc tccggcagtg agtttgtgga aatgttcgta
    11761 ggtgtaggag catcccgtgt acgtgatttg tttgaaaatg ccaagaagaa cactccctgt
    11821 ataatattta ttgatgaaat agatgccata ggtaaaagac gtgacaatca attaggttcc
    11881 aacgatgaaa gagaacaaac cttaaatcag cttctggctg aaatggacgg tttcgacagc
    11941 ggtaaaggaa tagttgttct ggctgccaca aaccgacctg aagtactgga taaggcattg
    12001 cttcgtccgg gacgttttga ccgacgcatt attgttgaca aacccgacct tatcggacgt
    12061 gaggctattc tgaaagtgca tgccgcaaag gtcaagctgg attccgatgt caatctgcgt
    12121 gaaatagcac tggcaaccag cggcgcaacc ggtgcggatc ttgccaatat gattaatgaa
    12181 ggagcattac tggctgtaaa agaaggcaga cagaaagtat cccagaaaga tttaatggaa
    12241 gcggtggaag ttgtcattgc aggtaaagag aagaaagatc gtgtaatgaa tcccaaggaa
    12301 cgtgagatgg tagcttatca cgaagtagga catgctttgg tcagcgccct tcagaaaaac
    12361 agccaacctg ttcagaagat tactatcgtt ccccgtacta tgggaagtct gggctataca
    12421 atgagtatgc cggaggaaga gcgctatctt atgaccgaat cggagattct tgctcagatt
    12481 accaccctgc tgggaggacg agctgccgag aatcttatat tcggagaagt aagtaccggt
    12541 gcatccaatg acatcgaacg ggcaaccaaa ctggcaaggg gtatgattac ccaatacggt
    12601 atgagcgaca agttcggacc tatgggactg gaaagcacac aaaatcagta tctggacggc
    12661 cgcaatgtag ctcatcactc ggaacagacc ggtgcattaa ttgataaaga ggtcagcagt
    12721 atacttgttc aatgtcagca gaatgctttc aatttactgc aggataaccg agatgctttg
    12781 gaaagaatat caaaatatct cttagaaaaa gaaaacataa cagggaagga gtttatgcaa
    12841 ctgcttgaag aaacacgttc aaaatgagtg ataaacaaaa aatacaggaa aagctctgag
    12901 tcaaacttgg agctttttta ttaaatattt ttactgctaa attcatccct tttgttttaa
    12961 aggaaataaa gtatctttgc ataaagaaag cggttaaaat aatttgctct tttattgtta
    13021 tattttgatg gttttgtgta tttattataa tccggctttc ttaatcaatt ctctaacaat
    13081 tgtacgatat tttataatat gcggttcaat atcttgctta gttgagttat atcgtatctt
    13141 atgagcagag tcattaccaa gatctttaaa caaactcata tcttttcgca cattcgagga
    13201 taaatgaagt ttaggatttt tctcagcatt ttttatgata tcacttaggg gcaaatagtg
    13261 tctcccactc tcatccataa tatcatcttc aagttcctta ttttcatacg taagaataag
    13321 aagattttct aacaaacgcc gcatgataac tgctgtgcag tcatataaat tgttttcata
    13381 acttgcgtta atttgcttac ataaagattg catatttggc gaattatgta aaaatccttc
    13441 aataacatca ttagttatta ttgattcatt atgcttaatg gcaacactgt cttcatctgg
    13501 ccaatccttt atattatcaa tccaatattc cataccagag gaccaatcag accagtcact
    13561 cgattgcgaa cacattttac tcacatcagt ttgtaaatca ttaataatca attcctctgt
    13621 aagaccttta cttttaagtg cactaatgta tcttatcaag atatcgttcg ctttatcagt
    13681 attccaatca ggaaatttat gccaacacct attttttatg gaaaatattt gtttatcaag
    13741 aggagtttta agcctgttgt aagcaattcc accaagtttt ttcataaagg tatcaactga
    13801 ttcctcagtt aaattcccct ctttgtcaaa cctaaaatat gcattaactc cggtatcaat
    13861 acattctttt aacaattcaa aggaaaaatt gttcactaat tttgaaacaa ttgatatgcc
    13921 ttcttcattg agagaatatg gagatatctt ttcatttatt attttttcaa tttcccttat
    13981 ttccattatt tcacctcgca atataatttt atactaaata tgtcaataac tctacaacta
    14041 ttgttttgat tatattttaa gataaaaaaa acagccatca atatgcacgg ctgttttctt
    14101 tattggttgc ggagaaagga tttgaacctt tgacctccgg gttatgagcc cgacgagctt
    14161 ccggactgct ctactccgcg gcatactggt accgaagacc ggggtcgaac cggtacagtc
    14221 tattcgaccg caggatttta agtcctgtgc gtctgcctat tccgccactt cggcatggct
    14281 ttagagactt ttcctattat atacaccact ttgtctattg tcaacagaaa cctatcttcc
    14341 tacagtcata tctatcttat tgagatactc tttattctta atattatcct gtatataggt
    14401 caccaaagac ttaaccatcc ggtcatcatc aatctccgaa aagattatat ctactaccga
    14461 ctgagcatag tcaggtttgc cctgtgagat atattgatac agaatttcgg tcattctggg
    14521 ggctatatac cctatagtat cctgacgaac agggatggat ttaaggataa taaatgcata
    14581 atcggttttt tgtgtctcca ggcgtaaatc ggtctgctcc aacttgtaaa acatgtcttc
    14641 catatcgtcc gaaaggaaag aaatacacac cgccgcatat tccggacggg aataaattga
    14701 ttccagtttt cttgaccatt ccttaattgc gtctttaacg ccgggctcca tgcgtataag
    14761 aagttttatc aatttatcat ttaattcata agctctatcc atatcatcct tgtaatacag
    14821 aagtcttatc gccagatcta ctgaatactc cgcaagtttg ggtaccgtat tgctgtttat
    14881 atccaccgat aagatgacat cggcagcctt atagtattct tctctataaa gcaacacata
    14941 tacaatgctt aagagatatt cctgagcttt atcatagttt ctggaatcat ctatttccac
    15001 aagcttgaac ttattatggg catcgtaata caattcggca agatacaact cccttgcttg
    15061 attataggct gttcttgatg cctgtattct ttcaacttcg ctttttgcat cctccagatt
    15121 atacagcggc atttccagca gcgaaaaaag attcagtttc ttttccgcgg tttcgtatga
    15181 ggtcttgtcc tgtttgaatg atgtaactat gttttcaata tattgataga catactccga
    15241 tgtattcatc gcatatacat aattcttttc aattttctca tactgtttgt tggcttttct
    15301 gtaatcaccc tgcattaaag tgtcaaccat gttaatccac ggctggttgt cacaacctgt
    15361 cagtataaag gataatataa aaaggcaaat tataagcagg catacttttt tcataaaaaa
    15421 agtatatccc atattgcctt ttcattcaat ataatgcgtt aaacagctcc ttgtaaaact
    15481 tctcttttgt aatcggatat atattctatg agacagcttg ctatgatttc atgccctttt
    15541 tcattgggat gaatgccgtc tctgcataac agttccatca gattttttgc atccaggaat
    15601 gccttcctta tatctataag ccggacatta agttgcttgg aaatatgttc tatggtaaga
    15661 gaataaagct cctgaaatct gtatatcttg aatacatcct taagccactt cagtatggct
    15721 tcttttgatg ttccgtcttt tgtaatccag tccagatatc tgacgggatc tatcgggtgc
    15781 aggttggtca gaaccggctc gatacctttt tctctgaaca tggtaaccat ctgccttatt
    15841 gtcttatcaa acactttaat aggggtattg gggtcatggt taatatccgg gtttgatgct
    15901 acttctttcc agttaaaatc gcagtcatta cctcccaatt caataagtgc aaaatccggt
    15961 ttaatatcat tttcttccag attcttcttc attctttcca aagccttggg tgcggtcata
    16021 ccgtatttgg aattattgac atatgtgata ttaaagtttt tagcggtttc cggtatgcaa
    16081 ctgttctgaa gaaaaacata cttaccggca ttgtcatcat acacaacgcc tctcaaaacc
    16141 gaatctcccc atattgatat attggtcata acaaaatcct ccccgtatta cattaaacta
    16201 tatacgcgga agatgaactt aatatgactt tttaagctta ttaagttaaa tcaatattaa
    16261 taatgtataa gaaaaaccta cttcttaaac aactccagca gaagattgtt gttatacagc
    16321 acacttgcca gtttgctttt ctgaattgtt tcatgaacca cactcattat ttccgctgtc
    16381 tggaaaccgg atataatcag gaatataatg taaatcaaca atacgcccga taccgctccg
    16441 ataatagttc ctactattgc atccacctgc ttgataaccg gcagttccga tatcttcttt
    16501 accaatcctc tgagaagttt gaatattatc aacaacagaa cgaataatat gatacctatg
    16561 attattgcta ttataaggtc ggttatgtta gcaaccaatc cgcccatagc ttccgctttt
    16621 gtggtggttt gagaaaaatc tattctctct ataagccaaa tctttatatc atcgggaaac
    16681 ggcaaagaat tgacggtagc gataatcgct tcttctaccg aatccagatt gggtaccaat
    16741 gcttccgcca tatgctcaat cttttcatgt atgttgttat agaaactggt tcccaccaat
    16801 aaatctctga tgggctgagc gaatttaaag gagagaaaaa tacttaagat ggtagtcagc
    16861 aatccatatg ccgacatgat gattcccctt attcctccca aaatcatgga aacaattatt
    16921 attaatatta agatatagtc tgctacattc atcatttatc tccttctcta tatattctcc
    16981 cacacttcat ataaatcatc aatccacagt tatctaatac ttataaacaa tcacattcct
    17041 tgacgttaat atggcaagtc tgtgactgcc tatcgctatt acctgttgta taagcgaatc
    17101 ggtttccact gaatggaaat catttccctt aagcgtaaac atgctcaagg tctgtgcttt
    17161 ccagattacc agtttattct cgaaaaccga aaatccctga acatcctctt gtaatgcttt
    17221 tttccatgcc aatgtgccgt cggaacctat cttaaatatc ctgtcttcac cgtctattat
    17281 accatatccg tatgcttggt tttcatatcc tttgatataa acaatctgtt gatctttaaa
    17341 aaccgaggtc ttttccatta ctttatctat cagcaaaaca tcggtatcat tggccacaag
    17401 aaatcttccg tcttgtaaac ataccgtttt gggcaggaac ccgtccaatg tgatacctgc
    17461 tatcggagtg ggtttcccta tttcaaatac ttctatatgt gtttttgaca tatcatcggg
    17521 gacacaggaa acaaccgcaa actgctcctt atcatcaaac atgtcaagat aaacgggata
    17581 tctttcggaa tattgtatat cgttcttgta tccgtcgggt attatgaggt ttgtctgata
    17641 tatcgaacag tataccgctc cgaggtatcc ttgtaagcct tcgcaaagta aaaacatata
    17701 ttcatcaaat gcgaatacat atgttatggc ggtttttgcc gtatattcat ctaccttctt
    17761 accttcacaa aaaacggtgt acgatgtggc acctttatca tataccacta catgcgtatc
    17821 ggaataggca atctttgtat cggtatagga aagaagataa gagcgtaaaa actctccgtt
    17881 gcttttgaaa atggataagt tgttgtcggt acagatatat atatacccgt tttccgatgc
    17941 aacaacactt cccgtcacta cgggaattgt gtattgtacg ccggcatctt ttgctactac
    18001 atcggcctta aagaacctga gtatctccgt cctgaaaaat atgaaaccga ccgataccga
    18061 taccgcaagc agtacgacaa ttataatcca gtaagctttt ttctttcgtt ttttagccaa
    18121 ttaaagatat ctcctatcac ttttatcaaa catattctac cacaatacat atactgctac
    18181 aaacaacatc agagcaaatt cgcaacagtt aacatattaa acagcatctg tgctatttcg
    18241 cttctttgta tctgaacttt aggctccacc ttaagtgcgg aatcaccgag tatgcctttt
    18301 ttatagcaaa aagccattga ttccattgcc caatccgaca cggtaacgta atcttcaaat
    18361 tgagataata catcccttat ctcagcattg ctcatctttg tatccagtcc gcataatgca
    18421 gctgcacgag caaccattgt agcggcttcc tgtcttgtta tagtgccttc gggattgaat
    18481 ttcccctctc cgacaccgtt tactatcccg tacttgttgg ctgttcctat ataacccgca
    18541 taccacttat ccgaagatac atcgctgaac actttggtat cctcttcaac aagacctaac
    18601 ccgcgggtta ttatggtagc aaactccgct ctggtcatat tggcatcggg tgcaaagctc
    18661 ttcttgcttc tcccgtttat gatacccctc gaagcaagcg cctctatcgc cgctatattg
    18721 ggactgaatg taatatcctc aaaagtggtg ccgggtgcgg ttatgggcat aggcaacaca
    18781 tctttatgct tcccctcgag accttcacct tttttaacag taatatcaat agaactttct
    18841 acagttacgg catcactcat tctgtaaaga ctgttcttcc cctcaatcgc tctaagagcc
    18901 gcaactacac cgtaaagccc ctgttccgaa gccatctgat tgctgccgga gccggaatat
    18961 gtatgaagaa aactgccgtc ttccaactga taagtcttta aattatccaa aagagtaaca
    19021 ccgtttttcg caaaacgtga atcgtccaaa ggtattccga gttcacacag tgcgacaagc
    19081 atttgtacgc agctttcgga atttgttgtt ccccagctgg caaaattgcc gctactaccc
    19141 tgcttaccgc tcatacatgc taaagcctct tttgttgctt tagctacttt aggttggtcc
    19201 tgatactttg caagagcttg aagtgccatt ccggtaatgt ccggatcgga aatttcgtcc
    19261 tgagaggtag ctatggaact tcctccgaac aaactccagc ctccgtcttt tagctgacac
    19321 tttaaaatgc ggtctatata cttctgtctt gtcgcctgag ttttggcttc tttgttttcc
    19381 ggaatgggat aatcctttgt atccaatgca attaaagccc atatgggtcc gttaagtccc
    19441 tgccatatag ttttatcgta atcacctaaa gcataagtaa ggtcatagcc tgcaacatca
    19501 cgggcatctt taccgattga agaaagagct acaataatac ggctgtattc cgtatatttc
    19561 ttttcatgca atactccctt acaagccttt acatagtttt ccacggtagc ataatagttc
    19621 tcgtaatatc cgtccggcac ctcataaccg ctgcgtgcta accctattac cgcccactct
    19681 cccccgatag accctacttg cggattcttt accgtattta ccatatattc agctgccgca
    19741 taaaccgact tttccaactc ttcttttgaa agagccgtta cgggagttgc aaaccccata
    19801 acaagagaaa gcactataaa caataaaact gttattctca atactgtttt cacaaataac
    19861 ctcctgcact gctactaaat agcagggtat ttgaaaccgt acacccgcca ccctgataat
    19921 cgggcgacgg gtgtactatt actttacatc attagtacag ttactcattt taattcatta
    19981 gtataaccgt ctacttcact acgtttttca taaaacgtgt aagcattaca gcagcttcgg
    20041 cacgtgttgt gttgcctgcg ggcactattc cggtgcgtgt acgaccgttt atgagttttt
    20101 caccgtttgc ccacttaagc gcacccaatg cccatttgct tatctctttt ttgtcaccga
    20161 atacggtaag gtctccggtt ttggatacat cataattctt gtactgtgca tatctgtaaa
    20221 gaatggttgc catctgctct cttgtgatgc tgtcattcgg agcaaatata ccttctccgt
    20281 aaccgttcac tatgccgtta cttgctgccc agcatattgc atctgtatac cacataccgt
    20341 ttttaacgtc ggtaaagatg ttcttactat ctgctttagg ctctttttcc aaacgataaa
    20401 gcattgtcac aagcattgct cgacttaatg tctctctggg ttcaaatgtg gtttcacttg
    20461 ttcctttcat tattccctta gcatagacat acttaatctc ttttacagcc cagtgtttat
    20521 tcaggtcggt aaatgccagt accacatcat ctaagagtac tacaaaagta cttaagtgag
    20581 ttacactgat acgtacatag agctttccgt caatctttat acactcacct tttatgtttt
    20641 ccgtggaacc gttctttgat atatgtacta cactgtaggt ttcacctgct tcaaactcgt
    20701 tacttacagg aatgtctatt gtaatgctct ttcctccgaa gcttgatatc tctttaccgt
    20761 cggaagatat acttaccgaa atcacaatac cgctggatgt atctatacta tcgtccgtaa
    20821 catccgctgc actcttttta gcaatactta cctcaatatc cgagccttca gcctcatttg
    20881 ctatggaagt taaggcatcg ttgggaatgg ttactgttcc gacaggagta tctatctcca
    20941 gacttaaatt cttctcatca gctatgctct ttgcagcagt agtcggtata tttacggcta
    21001 cattctttac attgtccgct cctgagggcg caatacttac acttcctgaa gagccgctct
    21061 ttactgcttt tactgcagag ttaatctgct catcggtaac cgaagcggta gcatcggaac
    21121 cggtaacatc tgctttaggt gtcagagttg ctacaccttg ctcagctgta tcggtatctt
    21181 ccactcccgg caggtatccg cctacatcct tgcccaagtc tcttgtgtat acccactcaa
    21241 ctacatcatt ttctttcaat tcatacaatg atgaagaata gccggggaac acaccgttaa
    21301 ccttgtacat ccaaccggac tgcggcccgt catcaaactc accgaatccg cttatagctt
    21361 caacataaac acctgcatac tgtgaatggt ccgatacccg aagagtcaaa cctgtgcgtc
    21421 tgagtattga atatgccgtc tcaccttcat tcatttccat tgctgtgggc ggatagtata
    21481 cgctctgttg gttagcctta gcattgggat cggttacact gagtctgaca tatttaagtt
    21541 gaacggcggg ttccgctatt ttcagtttcg ctattgctga aaccaatgaa ctaacggcat
    21601 tatccacaac cgcctgagtt acggtttcca agtctcttac agcttttgca tcccttagtt
    21661 caatttgtaa tgcactccaa cttcccgctg tataatcgct ctcactgtat ctttccgctt
    21721 gtgctatttt ctcttccaat gccgttttat ccgcaaccgc cggaagcaca tcatcggaaa
    21781 ccatcttcac aacatcactc atatcataaa ggcgtgcttt accgtttttg aagcggtcat
    21841 atgctaccaa tgcatatgca ccctgctcgc ttgacattaa attggctgca ctttcacgta
    21901 agaaagcctt gtttgtagca tcatagtagg aaagaagtat gctgatatag tttttaccgt
    21961 tgaaatcggc ggcatcttct cccaatgctg acaatgctac tattacctgt gaacaacctt
    22021 ccgaatcggt tatcttctgc ctgacaatcc atgcgagagc cttgccaaca gctgctgata
    22081 catcatcttg ctctttgtat ggggcaagag cctggatggt cattgccgta atgtccagat
    22141 tgggattggt attgtcaagc gaccaaccgt cgcctacttg gcagcttaaa atgtagtcga
    22201 ccaactcctg cctgaccgga ctgttctttg cgtacggtaa agaatccata gctatcaatg
    22261 caaatactgc accgtttatg ccctgtcttt ttacccactc gctatcggaa agtaacggag
    22321 cgataaggtt tttaccgttg acatttacag caccgattcc taaagaagtc aaagccagta
    22381 taagtcgcga gttttccgta gatttgttgt cattcaactt gtccggttta tccgctatac
    22441 ttgtcagtat gcgactgtaa taaccgtcat aatacggagt atctgaaaaa ccgccgcgcg
    22501 ctaacgaaag aatagcccat tcaccgccga tacttcccaa ttccggggtt tccacattac
    22561 ttctgatata gttaagtaca tcattcaatg cacccgcata accgtctttc aaaacaagac
    22621 gagtcattct gtcgttaaga tttgctaaag cattgtcaac ttttccctgt gcggcattcc
    22681 aatcgccttc aacttgtttt gcggcacgta atgcgttgaa aaactcatcc caaccctcga
    22741 tatagtggtc ttggttcttt gtttccgcca aagtaatcgc ttccctaagt gcgcctttat
    22801 cgcctctaag tacaagcata ctctctgacg ggaaagtaat tgcattcact tcttcaacag
    22861 tagttgccgc atcaatcgat gccattgttg tctggtagcc tctgcaatat gccgtccatg
    22921 aacccggtgt ataaggtaat tgatacagat atctatttct ttcctctatt tgtgctttct
    22981 tctccgcttt cgcagccgcc aactcctctt taagtgtcac cagtccgtca gttgacggga
    23041 tgcgaaccgc ttttacttct tccaccgatt cggcactgtt tacttcttcc tttgcattgt
    23101 taacagccgt ttgtaaggct gcccacgatg cttcagtgta ttcggcttgc actaaaccgt
    23161 caaagaccgc ttcaaatctt gcaattctcg ccgctttctc agaatccaat attgttacaa
    23221 gattctctgt cgaagggata gatattgctt ctaccgctgc taaagttttg gcattttgca
    23281 catcggctgt tgccgttgcc atcgctgact gcaatgcaga ccatgatgct ttggtatata
    23341 ttgattggtt taaaccgtcg gtaacggcag ctatttgggc aatcttcgcc tctttagccg
    23401 aagccaactg tgaagcaacc gctacaagac cgtcagttga cggtatagct acttccttta
    23461 ccgcaggaac atcggttgcg ttattgaccg cttgaatcgc atcgattatc gctgtctgca
    23521 atgctgccca tgaatcggct gtatagtcgg tttccgaaaa accgttggta acgccgttta
    23581 tctgagcaat ctttgctctc ttcacttctt ccaattccga acctgccgca acaagaccgc
    23641 ttattgaagg tatattaacc gcttccactg cggctgcatc cgtagcatcg gtaaccgctt
    23701 gaatcgcatc ggttattgcg gtctgtaatt ccgtccatga atcgggtgta taatcggcct
    23761 ccgtcaaacc gttgataacg gcatttatct tttcaatcct ggctgttttt agggttgcaa
    23821 gatgtgccgc aaccgtttca agaccgtctg ttgaaggtat tgcaacagca ttaatggcat
    23881 caactgttgt agctgcatgc acatttgcaa tggcactttg tatcaaagca taaaaacgtg
    23941 tccatgaatc ttttgtgtaa tcggtctcat cgacaccggt atatacggca ttaatttgat
    24001 caattcttgc ctgttttgcc tcctcaagtt gctgtgcaag gcttatagta accgttatat
    24061 caaagtcttt ggaaaagtct tcttttattt taccgttttc aattgttgca gtaaccgtaa
    24121 cattaccttc cgacaaggta tgtaaaatat ttttctctat gaatgcacct gtcgtaccct
    24181 gattcttaac agtccaggtt atttctttat gggatgcatt atccggctct accgtaccga
    24241 ctaatgacaa aggcgtctcc gcagcaactt cggcagggac atcaacaata tccgttacgg
    24301 aaacggatgt ttctatggta atcaatgcat tcagagcact tgacacctcg tattctccaa
    24361 cggtagtttg tttcaaaaca gtcattgcgt catcgtatgc agtaccgtat atctccgtta
    24421 tggcttcaat agaatttgaa tactgcttac gaatattagc aacctttctt ataaggtcac
    24481 tcctgttcgc tccgccgcct gtagcactgt aatgccctac gtatataaga cgcataacgt
    24541 cattgtcttc taacgtataa ccgtccatac cggttgaggg gaatctttca ttaacgcaat
    24601 acatccagcc gctgaaagaa cccgcatctt tttcgcttag gtatccctct ttctgcaccg
    24661 ttcccgagcc cttatacaca aagtccggat cccaaacacc tgcaagataa ccgccgttgt
    24721 accgatatgc ttcttcaccc ttttcgggat aacgtgcttt cagagcctgc atcataagat
    24781 ctgccgcatt aagaccttta ggcagtttca tttctgtcgg ttccacaata aaattgccgt
    24841 caataaacaa tttttccacc gtgatatatg tgataatata ctcaactgta ggtccggttg
    24901 cttcaccgcc ggcaataatg gtatttattg cttctattcc ggtatttttc acttcttcaa
    24961 tttcttttat actcggagcc atatttatgg ctgtaacggt ttcgttatac gtcgagtcaa
    25021 catttgaagc aatatcggct gtttctccgc ttgcgttttt gtaagcatcg gtttctttag
    25081 cagcttccca aacttcggtt aatgcattta ttgatgtttc tctcttctca tttacagctg
    25141 tagctgacga ccactctatt atgataaagc ctgcaatatg tgctacggca ggaccaagcc
    25201 caggcatagg ttgcattcca taccaataat acccgatagt gttttcttcc atgcaatcat
    25261 tgactatatc ttccggcaga aaaccttttg tgttctcata acactcttta cctatattgg
    25321 tctcggaaac tatacccaac cgaacaagct gtatcatttt gtattgaccg gaaggtgtac
    25381 gaagtacggg catcctgttg ccgagcatat cccgtccctt taactgtaca gagccatatt
    25441 tggaaccgtc aagggacagc ttatatatat ccattgtttg accgctctga gtaaatgaac
    25501 ctattttcgt tgccgttgta tccttaagct catatattgc tgttgcggaa aatacaccta
    25561 acgggcatat ttgcaggagt ataagcaggg ataaaataac agctaaaaac ttatttactt
    25621 ttatacttct ttgcattgtt aatctcctta tacaaattag tttcttcata ttaatactct
    25681 caagtgacta aatatcatca cctgaacctg tcatcttttg tggaaaatca tttgtaactg
    25741 tcaatatttc attttccgtg catcaacccc tttcttcatc gaatttagag accgctttgc
    25801 agcggggttc tttaaaagaa ctcgctccgc tgtttttacg attggtcttt tttccgtctt
    25861 cgacaaagga tgtgcttagg aatcagatga aaaaaccgca cgacagtaaa tatttaccat
    25921 cgtgcggctg tatcttcgca catcaaaaca ccatgcccct gtcatttatc gtgtgacggc
    25981 atgaattccg agcaggtctc ctgacttcgg tcctttttat gctctccctt gccttatcaa
    26041 atcataacga ttcaatgacc ggttttcacc gcgggagtgc ggaccttcac agtggcagcg
    26101 tccgtgccgg attccgaccg gcttcccttt taacatgtat gcgttcttat tccaaagaac
    26161 agaccaccgc atacagcact cgaaattatc tcctgaaatc ccgtaaacta ccgagacttc
    26221 aaactcaact aaaccatatc acaaagggta tgcaattgct accctttttt tacttttcaa
    26281 ctataaaaat acctcttttc cgattgccat aacgccttgc tcaatttctt taatactcgg
    26341 caaactgctc ctgtaatctt tcggaagaag ttgagataaa tgatattctg aaattccgat
    26401 aggttgactg ctggattcca aagcatattc agccacgaca ttatcctttg ttttacagat
    26461 taacaatccc aatgtttcat tatctgcttc ctttttcctc tgatgattca ttgcagcaac
    26521 ataaacacct aattgcccga tatagccggg ttcaaattcc gtcgccttga gttccacaac
    26581 aacatagcat ctaagctcca agtgataaaa caatagatca ataaacatct cttttgagcc
    26641 aaccataatc ggcacctgtc gccccacaaa agcaaagcct tgccccagct ctaataaaaa
    26701 ctgagtaata ttcgttgtca atgcatcctc taattctttt tccctatatc cttccgtcaa
    26761 ggtaaggaaa tcaaaattat atggatcttt taatgtttct tttgctaaat cactttgaga
    26821 atcaggcagg agagttgtaa aatttgtgat tgcctttcct tgtgttgtgt aaagagtcgt
    26881 atctaaataa ttgagaagta ctgctctgct ccaaccgttt ttaattgttt tctcaatgta
    26941 aaacaacgct tcttccggac ttttacattt tgtcattaac agaatatgat gtccccaagg
    27001 aattgagaat atatattcca attggtcaac agcctgttga cttttttggt aaaacaaata
    27061 aaagcgcttc atatacttaa ggttagtttc ggaaaaccct tgcagtttag gaaattcctt
    27121 gcacaaatct tttgacagtg acttgataaa tgcatcgccc caagcacact gttcctgtct
    27181 taatgcaatt tcctttccga aattccaata caagtccaaa agttcatagt tcacttttac
    27241 agcagctttt agttgactgc ttctcactct ttctttaagt tcacccaaga gtttttgata
    27301 catattgtcg tcaacaatac gcaaattatt gtgtgccaaa gaaacacctc cctatctcaa
    27361 gtagttaata tacttgaaaa tattatacca tttttaagat gccctatcta ttcgttttct
    27421 gatgataaca gaaaactgtt tggcagcttt ttatgttaaa gaagcaaaaa acttcacctt
    27481 tctataagtc cgtacttcag ttttaccctg tccaatttct ccagcatggg ttttgcaatg
    27541 gaaaacagga agacaacggt ggcaaccgca tggataaggt caaaaggaat accccggata
    27601 tatgcaacat agaacatttc cattgtgggt tttgcctcat acatcaatac cgatgcggga
    27661 ttcattatac ctccgtatat taagaatgta gccagtccgc cgaagataca aagtgcaata
    27721 gtattgcgcc gtaatattcc ttttctgaat aatatcccgg ccaaaaaccc tattataccg
    27781 aaagcaaaca tctgccaagg tgtccacgga ccctgaccga aaaacatatt ggacacaaag
    27841 gcggtaaccg ctcctactaa aaatcccgtc tcccctccga atgcaactcc cgctataatt
    27901 acaatagccg ctaccggctt gaattgcggt aacatgaaaa aagccgtacg ccccgccact
    27961 cctatggcac aaagcaccgc aattataatc aactctcttg cctgaggttt gcgcccttca
    28021 aatataagga caaaaggcag catggtttcc aatattatca gcatactgat aaaataatat
    28081 tttctgtcct ctaaataata gacgcctaag tagatagtta atgggatagc tataagtatc
    28141 attgcagcgg caagaagtgt acgcttggaa agctttcttt tatccgccgc tacctgttcg
    28201 ttatctatct ttgtatgttt aggccgaaga agaataaaga acaggagtaa ttccgctgcc
    28261 aatgcaacgg ctaacagaac atagtgccat atattcattt ctttaccgaa agaaactacc
    28321 gataaatcac cctgtttaat aatagctgac aaatccccca tgttgcttac ggcaaaatat
    28381 gccgttacaa gaaaggctaa agcagtaagt actgcacctg ctatacgcca ggcgggcaac
    28441 ttattattct ttttttcact ttttaataca cttttgtcat attcctctgc tgtatcttct
    28501 tttgtatcta tgggcataat gtttctatct tccggtatct ttatttcaaa aggttccgct
    28561 tttcccccgc acgcataaat tatatcttcc gcagttattg cggtcggaag catatgtctt
    28621 gccattctgt tggctgcggt ggtatagaaa ctgttgtcgg aaaagaaact tcgcggggta
    28681 ttccagctta ctatattacc gtcaaagaac agagcacatt tatgagcata ttttgcacaa
    28741 aactcaatat cgtggcttac catgagtacg gtaacgcctt tatacaaaag ctcataaaga
    28801 atattagcga atatccgttt aaattccgcg tccatgcctt tagtaggctc atctaaaagc
    28861 aatatcttag gctctaaaag aagaaccttg gctaaagccg cccgctgttg ttctccgcct
    28921 gacaaatcat aagggtgcat ggataacagc tcttccaatc tgcacagttt cgcgatcttt
    28981 gctatttgtt gctctttttc cgctttggac ttcttttgtc cggacagcat ttccaaaaga
    29041 tcctcctgca cggtcttttt aacaaaaagt gcttgaggat tttgcggtaa tacacccaac
    29101 atacggttaa acagttcttt atcccctatc ttggaccgtt ctttcccgtt tattctaacc
    29161 gttcctctgt aaggcttgtt tatgccggat atgagcgaca gtgccgtggt ttttcccgta
    29221 ccgtttccgc ccaatatggc atagaactct ccttgatata ttttcatgga aagaccttta
    29281 agcacatccg gagcatcctt ttcatatttg aaccatactt catcaaactc aataacggga
    29341 ataccctctt gctcctttat tgaatcctca gcagggatat ctcttaactg tttgttcttt
    29401 gtaaaggtat ccaaccatgt acgtccctct cttacggtaa cgggacaagc aagactattt
    29461 tccacacccg catagacacg catgggaaca ggcattgcta aaaacatgcc gtgatgtaag
    29521 tctttcaact gttcacccac ttcattaggt gtaccgtcac agattatccg cccttcatcc
    29581 ataaccaata ccctgtcgga tatagcgaaa acctcctcca acctatgctc ggttattata
    29641 acggtgacac ctaattctct gtttatcttt tccacagcgg agataaattc cgttgccgct
    29701 atggggtcta actgacttgt gggttcgtcc agtataagta ccgacggttg catcgccatg
    29761 atagctgcaa ggttcagcag ttgcttttgt cctccggata gttcggtaac atttttataa
    29821 aaccaggtct ctataccgaa aaatgaagcc atttcagcta ctttcaaacg gatagcgggt
    29881 gtattaaagc ctaaactttc caacccgaaa gccaattcat gccatacctt gtcggtaaca
    29941 atctgattat cgggagattg cattacaaaa cctatctttg aactttgggt acgtatatca
    30001 acttcagata acggtttccc ttcaaatatg atatctccgc ttaaacttcc atgcggtgca
    30061 agcacagttt ttagttgtct gagaagtgtg gttttaccgc accccgaagg tccgcaaaga
    30121 gttacgaact gtccccgctc tatggaaaaa gagagattat ttaatacttt tttctctttc
    30181 tccggatagg aaaaagttaa atttttgatt gtatagcttt ccattttcta tcctcattta
    30241 cattaataac aaccggcatt atacacaagg caaagtagca gatataaagg gatatgggat
    30301 aaagttcaaa ccccacacct ttcatagtgg gaaaatatct ccaatatata ccgcctaaaa
    30361 aagcccctaa tatgatatat attccgcaaa gagacaaaaa gagcatagct tttttatccc
    30421 gcttatcaaa acgatatatg gaaaaggctg tgcgtcccgg caaaccgtaa ccgcggcttt
    30481 tcatggaatc cgccgtttca atggcgtttt ccaacgccca tgtaaccata atggacagta
    30541 ttcgtatacc gtgctttgcc cgctgtaaaa tacttccgtt ggagacatcc cgccctatac
    30601 acttttgcgc atcggaaacc accttaatct gtgccttaaa atgtggaaca aaacgtaaag
    30661 ccatggataa tatcagcgac agagcaggaa taatgcgtcc gaaaaggtac acaaacttat
    30721 cggaagtcat aaccgcgtta tagcaggaaa accaggatat gacggtaata agcataaccg
    30781 ctgcggctat tccgtatgtt atggattcca gcgttaaagg attgccgtct ctaagatagg
    30841 taagaatggt gccgccttca tggttaaaag cggggtttat aagggcggta atgataagta
    30901 gcggtaacat atatttaaga ttgaagaata aagctttttt accgttcaaa taaattgaat
    30961 aacataaagc cgacagtaaa gatgttgcaa gacaaacagg gtgcataaaa aacatggaaa
    31021 acaccagtat taaagtgaaa tacagaaagt ttacggcagg gtggtatgtg gaaaacgtgt
    31081 ctttcatcat tcttccccca tagcgtaata tccacccaca tcgcgtccta aattacaggt
    31141 atatacccat tcaaccacat caccggcttt taactgataa cgggaagcgc cgtaattggg
    31201 aaaccaaccg ttcaccttat acatccagcc ggaaagctca cctacatcaa actcgtaaag
    31261 attgttgata ccttcaatat atacactgtt gtacatggga gtattcacaa actccatatg
    31321 aattcctgct ttcttcattt ctcttcgaag aatattaaaa accgattcac cctcatagaa
    31381 agttacttcg gtagccggga aaataactcc gtctttcggc acaagctcca ccttttcttt
    31441 gtctaaatat ttcatattat tcaatatggt atcgcaacgg acagaaagaa tgcaggtatg
    31501 tgcagtgtcg gttataacgg catcttgggg ttctacgggt atgggttttc cttcaggaac
    31561 agggtcggta agatatctgt ccttgcctgt ttccttatca aataccatac ccaactgttc
    31621 ggaataggat ttggaacctt caaaaacacc ctccgaagaa ccctctcttt gacaaggtgt
    31681 aggagaagga cttgcaaagt tatatgaaat atccgataca gcataattgc cgccccaaat
    31741 atatgttaca acgaggacac acagtataac tgctgctgca attatttttc ccttatgctt
    31801 ttttatgccc tttacctgca acctttatcc tcccgaatat ttgcacccgc cacatccctg
    31861 ttaaaaaacg gtcacaaccg caaaaaactg ttgcaaccgt attcatgcta aacgcactta
    31921 ctccaaaaag gagcacagca tcttcttgta ggtcttctga ctaacgcttc gcaagactaa
    31981 tagtctgcta accaaactct gccttcccgg tttcccagtg accgactttc gtcaacgagc
    32041 attgattatg ggcgcttaca gcggcggtac cgttcgggct ttgcacccga ttcccttgtt
    32101 cagtctcaca ttgtgagacc acaaaaagag atatttaatt tttaaagagc ttacataaac
    32161 tcttttcaat gtcttctaaa agacattacc gactttaatt aaatcataat taaggaagta
    32221 taacaacatc tttgtacgca tctttgtttg tttgacacac cggttttttt ctgttgacaa
    32281 aaatacgcct attttgtata ctcaataagc gacctaaaaa tgaaggtctg attgtacttt
    32341 tttaagagaa tatattgtaa tgatctggtt aagagcaatt tttacccgtt atgcggcctg
    32401 gtagttcagt tggttagaat gccagcctgt cacgctggag gtcgtgggtt cgagccccat
    32461 ccaggtcgcc atatgcccag atagctcagt cggtagagca ggagactgaa aatctccgtg
    32521 tcggtggttc gattccgcct ctgggcacca aacgagcggg tttaactcag cggtagagtg
    32581 tcaccttgcc aaggtgaaag tcgcgagttc gaatctcgta acccgctcca tttatttatg
    32641 gcgccataac caagcggtaa ggtagaggtc tgcaaaacct tcattctccg gttcaaatcc
    32701 ggatggcgcc tccagattac ggtagacgga cacaaaagcg tgttcgtctt ttttatttat
    32761 atcatgtagc taatacatct ttcagcatta cttgcctggc aattgattaa tctaaaccgt
    32821 acaaagaacg caattcttcc aatatagtgt ctttggtctt attcggtata cgaagcttta
    32881 tcaaatccac atcttccgtt tccattttct ctaaaaaggt tctcatatct ttcagcactg
    32941 taagcggcac attccgaagc ggctcttgat cagggtaaag cagctccgtt aagcgaaata
    33001 catcattctt atgtttcttt atatccctgc tgtctacatc accgcaggct acatttcttt
    33061 cagacaactc caaccatgcc ttagctttaa aagcaatcag atgagttacg tctaaaacag
    33121 ttactcctgc aacctttgtt cttccctgca ttaaaaaatt atagtaatca tcatcaagaa
    33181 gaatagccga tagactggaa atattttcat ctaacgggag cggtgtaaga atagcatttc
    33241 gaggcagaat gattgtgtcc ggttttcttg taaacagttc aatcattgca gggtattctt
    33301 gcaattgcgg ctttgaaaag cggtaaaatt gatatgcttc cgtgcttttg ttgcgatgtt
    33361 cataccctgc agcaactatg tattcccaaa atctttttcc gaacgaaaca tctaatgctt
    33421 ctataaccaa tacaagatca atatcttttg tcgcacgaaa atccaaccct tcatttgtca
    33481 ttaacaggtc acaagcagtc ccccctataa tcacatattg ctcttcacag ccttggaacc
    33541 attttctaaa gctttcaatt cctcttatca tcaatatccc tccatacctg ctcaagcatt
    33601 tcctgcacag cttcctctac cctttcgtcc ttatcttccc gaagagataa tgcaagagaa
    33661 agcctgtcaa cattttcgtc cttggatagt tttctcggat tataactcca aaattccact
    33721 gcacattgct catccttgtt tagcaacttc tttgaagcaa tcttctccca tcttgcaata
    33781 ctgtctgtag cataaaagtc aatgtcagac ggattcaaca tagaataatt ggaaagagca
    33841 gagtatccgc taaaaagcaa tctttctttt atctcacttt tcggtacata tactcttctc
    33901 tttaccggat tagccaggtg tttttgccct tcagtaaaga gctcttgcac agttttatcg
    33961 gaaaaaacaa ctttctgtac acctcttttt tctgtcttaa ttaatcccat accctcaagt
    34021 tgtattgatg ctctggagat agatgtcggt gttaattcca atgctttggc agcttcactg
    34081 gtcagcagct caccgcaacc gttataaatg tagtatagaa acagcagttg tgcggaagga
    34141 agcattacat ccgtcatttg cttttctcca tcgcttcgct cctgaagata agttcccata
    34201 aaaggaaggt atatctgttt ttcctccaca ataaacggta tacgatcgcg cagaagatat
    34261 tctttctgcc tatatgtcag tttcttagga acaagaataa catgcaaatt ttcttctttc
    34321 tgtatcctct ctatatgttt tttaatagcg cttacaggtt ccaattcagt cttcgggaat
    34381 aggaatataa cttcatttcc gtcaagagtg gctttctgca gcttatatcg tgcatgaata
    34441 aaattgggta tattcctcac aatatcgccc ttatatacta catgtattcc aagtaaagta
    34501 ttcaaatatc ccatcacttc cgccctttct taactctgat ttgtattata actcaattta
    34561 agttataatg caataactaa gttaagatat ggattaataa tcaactaaaa cctaattagc
    34621 tacacctttt tagtttttaa gtgaccctgt gttagtatca ggctaccaca ggaacattaa
    34681 cctcattttt ggaaatttgc tatattatca tgatttgcag gtaaaaacca ataatctgca
    34741 tgtaatcagg caataatagt gcagaatact tgcatatcct gcaccgaata ttacactaca
    34801 ttcggtgcag tattaatttg ttataacggt ttctgtttat cggtcggaaa aactttccgc
    34861 tgtcttccga atatgattct caaatgcggt ctcttctata aggtaacggt ttttcattat
    34921 attccataat tgatccgctc cgtttccggc tcggactttc aatgtctcta tgcaggaaag
    34981 tgccacctct tcacttatta ctttcctctt cgatgaggct tccatttttc ttttaatata
    35041 ctcctttgat acatgtgaaa agatatcaga tgcatcttct tttgtcactc ctattgcacc
    35101 tgcaaggctt acggcagacg gaatatccga gaaaaagtcg catacaagat aatatccgtc
    35161 aatgctctcg cacagcaaat ccttgggata acaaatccaa caatcggcat atttgtatgt
    35221 ctctctcacg tcatctttgt cgtcagccgt gacatagcag aataaaagat agcgtgctcc
    35281 ttccgttatt acgggaagtt cttcgttgta tgtactcata gtgtaaccta cacctactgt
    35341 aatgcaatcc cggtcgggat acaaatccga tgtacaggat aaaactttgt ctaccttaat
    35401 atcgaacagt gtggctttgt cggaaacatt ggtatccatg aattcatagt ctacggtagt
    35461 atatcgcaca tttgaaactt caccggtcaa tattaccgtg ttttttgtat aagcatcttc
    35521 tttcttaaga acaacccagt tataatcaac aaagacaact gtttgcgtat cttcaacagt
    35581 ccggaattct gtgctgtccg atgcttccgg ttgcttttct ttgttaatct gacaggcaac
    35641 taaaagtgag ataaatacta tacagcaaac aatgtataat aactttgttt tcttcattat
    35701 ggtacctccc gatttctatg cttttatttc accttgcaca gtaccacata accctcaatg
    35761 tttgaagcta tgcgcgaata ctgtactgtt tccattacat tttcatctat gtatatgtta
    35821 tcattttttg cgtatatatg tctatatgtt tcattatcgt atgtaaaatc tatgagcttt
    35881 tcggtcaaca gtttttgata aaactcctcc atgcataagt tctcattgta aaggtaaaat
    35941 gcggtgggta atcctacata ccgtaagtgc cagggctcat ttaaagtaat ttttgtatac
    36001 tgctccttgc cttcttcata tcttacggta aatccgtatt tgtaactgtt gtttttaagc
    36061 cagattgcat acggagcacc ttttgacgat tcatccatca tgtagccgta acctaccaca
    36121 tccaatgtaa gtccgttatg atgttcactg cagccgggta ttgctacacg tcccgctgtg
    36181 tattcatagg cctgtgcata tgtcatattt tcctgagtca cccttctgtt gacccagtaa
    36241 tcaaaaagct gcttctgcga ctcatacaac ctgtgtgcac ttgcaacatg cattttaaga
    36301 cttgcatcct gcttatcggc agctgctaat agttcttcca atgcctgttt aacatgaggc
    36361 agaatgaaaa ggtcatctct gttgtctaaa acatagtttt tatccttaag ctgtataagt
    36421 cccgttgtat cgtttgcttc gtcttgcgtt atagggttat ccctgtttat gattctcagg
    36481 g
//
LOCUS       DAOSOM010000020        34098 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_1303_length_34098_cov_6.017830, whole genome shotgun sequence.
ACCESSION   DAOSOM010000020 DAOSOM010000000
VERSION     DAOSOM010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 34098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34098
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1303_length_34098_cov_6.017830"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="PLF14_06030"
     CDS             <1..580
                     /locus_tag="PLF14_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70883.1"
                     /translation="LAKRLGVPCCPATRADKDTVFSTFLYDFSKEYIVHFRRLFKGER
                     SDNEYRNLIEVRPKFKEDISRMILLDFITRQDDRHLSNMAIKISGNKESFYPLYDNGR
                     SLFYEDTEETVEKAIKDIAGYSTVFGYSGTYWEHVQDIAAKINIADLINLNVTKAEVE
                     IILKQAGFVSYRLEGATEWVVKSINMLKEINR"
     gene            813..2774
                     /locus_tag="PLF14_06035"
     CDS             813..2774
                     /locus_tag="PLF14_06035"
                     /inference="COORDINATES: protein motif:HMM:NF020730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70884.1"
                     /translation="MKKRFIVQFVAIFVLLSVLVAPVFTGYAKGDTQDSQSEASSEVT
                     EISWDNRNTIHACVSVGVIRGEGGLIEDEYWLRNTTRAQGLLIGLRLRGLEDSARFYK
                     GTYSFNDMPGLNKAYWSPILAYAYEHPELGLVTDVKSGFRPSEVLTGNEFAKIIMEAL
                     GYKQGVDFESADVRSFAQSKGLTVKEGKVTNQDFTVVLQQALKLNNKEGKLFIEDLYQ
                     KGALYDRREVKAAPGTDPAIPYAARMRLFYYEGGWLQLHASDQYGQRILPPKGTFSSS
                     DTSKIVLGDQRPGVIATTDVSVDAYGVTSGINTAVVTFKSDDGKWNLSTEVTVVGKSD
                     VNVFTDIRYEQDYQALMVGKTMKLRKFSLIDSLGNFVNLSFHQYYLEVRRADDNDPYG
                     TGDSAVFEIGRQEINEHYTYITAKKAGTETLKIVACKGGMPGSGNPNYIEAVKEFTLT
                     AVNDTDIVGCKVDFLYDKNFMYAGELSEDHNLKIKVSGATVNRMIVQIQSTDEVALPD
                     AFSYKVDVDNIAVSTEKSEIVTVRPVTKDMEAKIKVIGKRGYVIGVFPFHLKVDAPKV
                     HYLYMSAFNKDYVYIYAYDQYGKSIAVPEGTITSSDPDKIAITGTREGYNPYDLCYNI
                     SSVGDGPNTATITYVTDDGLFKAEVTVTR"
     gene            complement(3015..3548)
                     /locus_tag="PLF14_06040"
     CDS             complement(3015..3548)
                     /locus_tag="PLF14_06040"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HOT70885.1"
                     /translation="MNKMMTRTKQIAYTGIFTAIIAVMSQLAIPMPSGVPATLQTLAI
                     AITGLLLGIKAGTLSVCLYITIGTIGIPVFSGLRSGFGTLFGVTGGFIFGFIPFVLLC
                     AAAKNVKNRIAAIAICIAGLLTCHLCGIIQFMIITGKPFIQTALTVSLPYLLKDIVSC
                     ILAYIISVKLSKVIAVE"
     gene            3628..6075
                     /locus_tag="PLF14_06045"
     CDS             3628..6075
                     /locus_tag="PLF14_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOT70886.1"
                     /translation="MSRVCQNCRLRVSDKAKYCRKCGSPIGIANVPEYSAAENMMQFS
                     KPLEKSRNRLGLTGLKYMLIDLRKGLRRTTYLIPVFAVAVIWIAVSLLFSAGIDTVFL
                     RMLSFFTYADAGMNGGFLGLTGGILGKMAYAYFIVMLLTPLYSLQKPFKGVIPGAKTY
                     ISQSFRFKTLKDVYVFILGMGFSFMAYNMLTGDNAWTKSIISLIIAVIFFRGMGKKYG
                     FTWDVFHRVFSDSKDEDITVKAMGGYVTGLLLAVPTFLFDSSFLSYYIGIVLIVIFLV
                     LKFTEFIIVHKADFKRIFPIILVCLILFYPLSALSAPATSEIRGVWILQDVKLVNPDT
                     KKNPKDNTYVSDGYIRKQYSADSQYTIEYFWKSLPKKISAGEESRVMIETKLVYTSAE
                     ETALKTHLNSAMFITYKPNPKEPEVNKYFPGSRDFSVEGVFSELQQEHTAFFLKITDY
                     YELYDLLTKNNCHLTVSVQASFGGRVEYIYRLQRTPVDGFLTWLAGDGTAYDHFGPLG
                     TVFSNVASVTASMLCAAVAVGSLTLKRKKGLLTDIKRTDKKEEIPEEKYTDIRTESDF
                     ADNLVNLYETFSNAFAAGWPKYREVLIKTLSRSKDNAVAYKSVGAIESSINNYFNSLS
                     GITNSLAALGYNNDSLGLQQRGEEITDATGKIVYANYVFQSLASGTSYSAFMELANAF
                     ALENTKAGVIASPSIHLRGIMSLVLDSISDAQNAYKNAVNGRYGEKIRSMVKDGELTY
                     ESIQAFADTVIKADETDRFYERMLTGTVMPEDRKYLSSYTACATERAAKQAQGAAFRH
                     ENTSLVVSSLFDWQEEE"
     gene            6075..6992
                     /locus_tag="PLF14_06050"
     CDS             6075..6992
                     /locus_tag="PLF14_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70887.1"
                     /translation="MFYILSAHDYSALIALSDIKQNYISQAVYNPDINSKTSFTRAKS
                     ELSKLGLVKKGVITTTGRVIMEAVLDPERVVSVVSCNSIDMATAHYCFSKGFWLRLLP
                     KNDHSIITIESPIEEKDLVQKIRRDFLQDFDFTDTQPIDVFLTVKEVVIFQLMQLAIV
                     DKVRKKGAPLTRAAAALGISEFITNKNVLNAAAAAYMLGEKAGYELSRQFWDTNNVIS
                     HIDALVGKNIFDKVIEQGTGKLKYIMSDISRKWLTLDCLVDKITIQKLPQGDIINVSI
                     TKSGMMTVTQNAEGLRFKTITINEFYEEK"
     gene            6994..7464
                     /locus_tag="PLF14_06055"
     CDS             6994..7464
                     /locus_tag="PLF14_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOT70888.1"
                     /translation="MKKYVRDSYTEQAQILTQSALNGNDRLFGGRIMEWIDITAAIAA
                     RRHSNRNVTTVAVDSLEFKGPAYANDTVLLTAKVTYVGKTSMEVCVKSYVEELNGEKK
                     LINVAYVVMVALDENERPVEVPGLILETEEERCDWEMGKKRYLLRKQRKSNAGC"
     gene            7620..8417
                     /locus_tag="PLF14_06060"
     CDS             7620..8417
                     /locus_tag="PLF14_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOT70889.1"
                     /translation="MQGITIGQYIPGDSIIHKADPRLKIIMTFFTMILLLIIDSFYPL
                     LLSAVFVVTAYLVSGISLRYAIKGLKPVIYIVIFTMILNIFFATGTPLFTLGFITVTK
                     EGLVTSALVSARILLLISAGSLMTYTTTPLVFTVGIEKLMYPLKYLKVPIAEIAMMIS
                     IALRFIPTLMEETEKIIKAQAARGADIGTGNIIARTKSFIPVLVPLFISSFKRADDLA
                     TAMDARCYNGSEGRTSISELKYGKIDLAIFMVYILFVAAALVLQYVL"
     gene            8428..9375
                     /locus_tag="PLF14_06065"
     CDS             8428..9375
                     /locus_tag="PLF14_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOT70890.1"
                     /translation="MYSIGIDLGGTAIKAGIVDKSGNIIVKDKVPTKSERGYEAVLRD
                     MAKLCKKLIEKAGMSENDIEKIGVGCPGTPDKVNGILIYNNNLGMRNVNIRKELQKYF
                     SLPVYIDNDANVAALAESVCGAVKGTYCSVTITLGTGIGSGVVIDNKVYSGFNYAAAE
                     IGHHTLVMDGEPCTCGRNGCYEAYASATALIAQTKKAALANRDSKIWEACGHNLDNIS
                     ARTAFEAERMGDSVAKNVCDTYIRYIGEGLVNIINIFQPEVIALGGGVCNEGDRLLIP
                     LREYILKYKYTREETPSSTLVIAQMGNDAGVVGAAMLTE"
     gene            9383..11014
                     /locus_tag="PLF14_06070"
     CDS             9383..11014
                     /locus_tag="PLF14_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70891.1"
                     /translation="MKKHYLGNVKSNEATIDTYFRIYDPAIRAFRNSYGYASHAMHTT
                     PMDSLAAGYYCKDNKYYKDPKLLELMHNVLAFMESHHRPNFTIDMMESNFFCSPLFEA
                     VNISRAYRIMQKYASTDDEKKLLKRFTDYLPMMEKSLLNGGIHTPNHRWIGASGMLML
                     ANIMDSTDLRNLAFAYLNEGVDMNEFGEYTERSPHYSEVCNTSFMQIALETGDRSYLE
                     YAKKNMDLLFRYLEPNFTMFTQNSNRVDTHEGNVNAVTKPYYLYYTYLHMSYLTNNPQ
                     YAKFADDIYNAVEKIHGTGPGSLWMYLAYPELMEYEPKMEQYPTSYNIFHGDIVRYRN
                     EDFSASILTRSPNFLFVQTGDLRCFVRICASFFAVAQFKPLSIELIGKDTYRMKMKAH
                     GEYYGPLPEKPATTAWKEMDHSLRPVNNKVDLEYIVTVKIKPDRVSLNVKTEGCDNVP
                     CKIEFCITPPVDVQYDSVELKGLPDECLSVNAKEIKIKKENDCLTLTNSFSEHTYHKD
                     MRGSVPPSKGVFTVYYTSFTHIDASVDIIGTKETK"
     gene            11011..12645
                     /locus_tag="PLF14_06075"
     CDS             11011..12645
                     /locus_tag="PLF14_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70892.1"
                     /translation="MKSHYPDIVKGNEAAIENDLKRFNPDERIIYGAYGFPDYANNAV
                     PIETMSSGYYCKDSKFYRDKNLLYIMDCLIDYLYSAQREDYTVDTKISDYKTGPVLLS
                     NFLVRGYRIMKKYAKTEEEIALRDRLKAYLVPVAKGVCNGGTITPNHRWMYSSSALML
                     YNEVKMDCLKEISEAYLNEGVDIDEAGEYTERSPMYNRECNNAFIIIAEETKNDYYLE
                     SVRKNLELMLYYIEPDFTMFTQNSNRKDKQEGDVSATFYPDGYYHSYLHMSYLDKNPL
                     FAGMADEIFNSIKERTGRGPSVLWMYLLNPKLKEYEPKTVTRPASFNIFHKDIVRRKN
                     EDFSVTLLTRSPNFLFVQKGNLRCFVRVCASFFAVAQLIPQKIEKTGDDTYRFTMTAE
                     GKYHGPLPEKPNTVVWKEMDHSKRPVINRVQLTYHVTIALKDTGVCMDIKSEGMDQVP
                     YKVEFCFTAPCTVKNKDKRYDCRPGEHMTFNEGDIDIEKDGNILTITNTHNSHIYHDI
                     MRGSVPKSASSFTIYYTGFTHINEHIEILNQRRENA"
     gene            12638..13555
                     /locus_tag="PLF14_06080"
     CDS             12638..13555
                     /locus_tag="PLF14_06080"
                     /inference="COORDINATES: protein motif:HMM:NF013078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HOT70893.1"
                     /translation="MPDILTHILFAKNVKDAIGDINVKDAIKNNMQLYNFGAQGPDFL
                     YYHITPLFFDNRVPAAGSMMHRIETAKFFKDFVLWLQNLDGSAYQQGLAYFAGFLTHF
                     YCDKTLHPYVLATVKQGANYFNSSTGKVYLSHYMVEYIMDIRLWREKIGIDAYKQDIT
                     ELIGTEPLPYEIVEYITEFINYTQPNAVTKKEVYNSAVKMIRTQKVFYDPKNIKKWWI
                     NLLPLPKKCYVEKVKEDVDVLNVNGRIWSHVMNEDEKSDSSVYDLMEIGCNECVKVLD
                     EISEMLERGIGKDLNKLIPDVGYLTNKPV"
     gene            13848..14951
                     /locus_tag="PLF14_06085"
     CDS             13848..14951
                     /locus_tag="PLF14_06085"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOT70894.1"
                     /translation="MKTTMKESEKLYRKTIINFIILFVACQIAFLIVLSIVYGIFLKY
                     PFLCRIENDGGVTITKYFAKEKKVDIPEYITGKPVTGIGFKVFLDNKKLTEIILPSTL
                     KWIGGEAFSGCWCIKNIDLPDNLIAIGDEAFRGCSDIKNINLPDNLESIGSRAFKDCR
                     FDYIKIPKSVVRMGSGVFSGSSLKTVELSSLLPEHTFSGCTELQHVIIPDGIERLEEY
                     TFSGCSSLDNVVIPESVIAIDWRCFSNCTNLKKMIIPEKIETIPYACFADCVNLTDLT
                     ISDGVLRICGNAFEGCIGLLEVEIPNSVTYIGERAFSGCKNLKKITILQNVTEISHGA
                     FDGCDNLIIYCHSNSYAEKYAIIKGISYVLIDK"
     gene            15042..17039
                     /locus_tag="PLF14_06090"
     CDS             15042..17039
                     /locus_tag="PLF14_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007493288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOT70895.1"
                     /translation="MAVKNTYNNESIKSLKGPDRVRKCAPVIFGSDGLEGCQHSVFEI
                     VSNSVDEARAGHGNLIEVIRHADKSVTVKDYGRGMPVDFNKNEGRYNWDLIYCELYAG
                     AKYDGNLSGNYKASIGLNGLGCCATQYASEYMDVEVIRDGFKYNLHFEEGYNKGGLKK
                     KEDKSRKTGTTTRWKPDPTVFTDIDIPLEYFQDMLKRQAVANAGLKFTLKDEQSGQTF
                     EYVYENGIRQYLEEVVADKNCTTPVFISEDTKLVEKDGSREHDARFTACFCFSSDMSL
                     IEHYYNSSYLVNGGSPERAMRAAFAYALDKFITTEGKYTRGEAKITYTDIQDSLVFIS
                     NSFSSVGGFENQTKKCLVNSIFQDAFTEFLKHELEVYFIENKSEAVKIADQVLLNKRS
                     AEQAEKTKLNLKKKLGKGLDMNNRVKKFVDCRSKDADKREIYIVEGDSALGSAKMGRD
                     AEFQAIIPVRGKILNCLKAEYNRIFDSDIIIDLLKVLGCGVEIKTKANKKISQFDMKN
                     LKWSKIIICTDADVDGFQIRTLILTMIYRLMPSLIDAGKVYIAESPLFEITHKKDTYF
                     AYSEAEKNEIIEEIKGNEKKNGQSPRITIQRSKGLGENEPDMMWLTTMNPATRRLIKV
                     MPTDVKDMDYMFDLLLGDNLQGRKDYIQEYGYKYTDMLDVS"
     gene            17127..19259
                     /locus_tag="PLF14_06095"
     CDS             17127..19259
                     /locus_tag="PLF14_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HOT70896.1"
                     /translation="MPYAMSVIVSRAIPEIDGLKPSHRKLLYTMYKMGLLKGDRTKSA
                     NVVGQTMKLNPHGDQAIYETMVRMTRGNEALLHPFIDSKGNFGKQYSRDMSYAASRYT
                     EVKLAPISAEMFRDIDKDCVPFTPNYDGTLMEPVMLPSAFPNILVNASQGIAVGIASN
                     LPSFNLAEVCDCIIAYLKDRDIYLGDYLKCLDFSTGGYAIYDKAEMDNIFTNGRGRIR
                     LRAKYNTDKKGTHIEIVEIPYTTSAEAIIESIIALVKSSKIKEITDVRDETDISGLKI
                     TIDVKRGTDIERLMAILYAKTPLEDGFSCNNNLLIEGKPLLLGIRDIIDYWIMFRVEC
                     IRRQLRYDLAKDRERLHLLLGLERVLLDIDEAIRIIRNTEMESEVVPNLMEAFRIDNL
                     QAEFIAEIKLRHLNREYILKRLDDIKDLNKEIKEAEETLKSRTKIENIISKQLTEIKE
                     KYGQPRKTEIIYEEQIVEEFEEPEFEDYPVKIFFTKDNYLKKIREVDFKTDVENKFKS
                     DDVLLQEFSSNNGAELIMFSNQGNVYKSMLHRIDDCKMSDLGEYLPNICEMEKDERII
                     YVVATADFKGHMIYLFENGKGARVPIETFRTKQNRKKMLNAYYMGSKLLYLALIDEKD
                     QSSYVAITSDQRALIFSAADISVKEKRDTQGITLIKLKPDTKVTEMLPLEMARLKDTD
                     RYKPANVPAVGKVLSDKDKKRKQISLFD"
     gene            19269..20141
                     /gene="galU"
                     /locus_tag="PLF14_06100"
     CDS             19269..20141
                     /gene="galU"
                     /locus_tag="PLF14_06100"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HOT70897.1"
                     /translation="MKKIRKAIIPAAGLGTRFLPATKATPKEMLPVVDTPAIQYIVEE
                     AAQSGIQDIVIISGRGKRAIEDHFDKSYELEDVLLKHNKIAELEDIKSISDMANIYYV
                     RQKEAKGLGHAIHCAKTFIGNEPFAVLLGDDLVYSEKPCLSQLIKAYEQYQASIVGVQ
                     SVDKDKVSLYGIIDGEEIAPKVMKVKDMVEKPDKAAAPSNVAILGRYILEPEIFDILE
                     HQKPGKNGEIQLTDALRTLLTIQDIYSYEFDGIRYDLGDKLGFVQATIEYALRRDDLK
                     DNLKAYLKDISKKL"
     gene            complement(20218..21603)
                     /locus_tag="PLF14_06105"
     CDS             complement(20218..21603)
                     /locus_tag="PLF14_06105"
                     /inference="COORDINATES: protein motif:HMM:NF025644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHHW family protein"
                     /protein_id="HOT70898.1"
                     /translation="MKKLCIGSLLFIIVIFAAGIINIVNPNKPSVSLAENRKLQEKPV
                     FSVSSFFDKSYANNYSAYYSDTFIQRDTLIQYRRAADKFFGFKKNMKIEISKPVNTPS
                     PSALPINTVTYSPSPSPANTCTPATSQTEAPPSPTASVLPTPSPTPKPEYRAASAIEV
                     LKSPFFLVDNNVVATIWAKDESLKKYAMFINELKDSMGEHVNVYSMVIPTAMEYFNLS
                     DMQYITGSQKQMVEDIAQYTNDSVIDVDAYNALFHHCKEYIYYKTDHHWTHLGAYYAY
                     TALMQAMGKEQDIVPLESYVDREVIEGFIGSAYRKTNQDERVLNNPDTLIAYYPLVEY
                     KYIMHKKTGPIEKRLNDLAYVNPNNQYYNMFMSGGNGVYHEFTTENKNGRTILVICDS
                     YGASFVHFLLPHYEKVYIMDTRFYDREYLNNMTIDTFAKSIHADDITTLLYMDSVAYT
                     FNTDALYGLLK"
     gene            complement(21605..23026)
                     /locus_tag="PLF14_06110"
     CDS             complement(21605..23026)
                     /locus_tag="PLF14_06110"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOT70899.1"
                     /translation="MVFSGLFFLTVFLPVSLILYYISKNRTYRNIVLTVMSLVFYAWG
                     EPVYILLLLLTSAFVYIVALLIQQKAGTRSAKIYLITGITVIIAVLVFFKYSGFIAQN
                     LSAIFKFKNPFNDIALPLGLSFYTFTCISYLADVYKKKTEAQRNFLDLLMYISVFFTV
                     TSGPIRRYPDWVADIRSDRETWEKFNYGVHRFIIGLAKKAVIANTAGTLISSFTGTDY
                     SRLSVQGAWIGILLFSLQIYFDFSGYSDMAIGLASMFGFSVNENFNYPYISKSATEFW
                     RRWHITLGGFFRDYVYIPLGGNRKYRIRNLLVVWFLTGLWHGASWNFVIWGLYFFVLL
                     LIEKAFLIKLFEKIPGIFSHIYFLLVMLIGWVLFYHTDLSQAVRYIGVMFGIKAASFS
                     DPILTILFNNNVLFILTAVICTVPLGKFLKQQAEKLEYRLSKGHTDILNGIMIPLFNA
                     ALLILSIIFLVGDTYTPFLYFNF"
     gene            complement(23026..23745)
                     /locus_tag="PLF14_06115"
     CDS             complement(23026..23745)
                     /locus_tag="PLF14_06115"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis TtcA family
                     protein"
                     /protein_id="HOT70900.1"
                     /translation="MSGLVISQMRKAIHDYDMIQDEDTIAVGLSGGKDSLALLYGLSK
                     IRGHIEQSFDIKAITLKLSEENPDTDYLKKFCDNIDVELVVKQTDIEQIVFEERKEKS
                     PCSLCANLRRGMLNDTAKTLGCNKVALGHNKDDAIETFILCLQYEGRLHTFAPVTYLS
                     RKDITVIRPLIYSFEQDITAFNESAGITPVKTLCPAAGNTHRQRIKEYIENESSINPN
                     FKYNLFRSFTHANISGWKEVL"
     gene            complement(23781..24977)
                     /locus_tag="PLF14_06120"
     CDS             complement(23781..24977)
                     /locus_tag="PLF14_06120"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOT70901.1"
                     /translation="MKYLTWFKAVYAVLRIPFTVYYKYFHNYHYKKYKPKNKPFILLS
                     NHNSDGDQFAVGVAIKGHMFYVASEHILRSGLGGKLVRLLAYPVVRKKGAEAKSTVKD
                     IHKRLEQGANVCMFVEGNRSFNGQTGWISPTNGALVKHSDAALITYRLDGGYFRTPRW
                     AKHARKGPEFGHVVNEYSREQLQSMTEEEITDIIRTDLYTNAFDWQRKYKQRYKGKNL
                     AEYLETVLFVCPKCKAIDTLKSAGDIFSCTCGLSVKLNEYCCFEVQSDDEFDFENVYD
                     WDMWQREYLKNNLDKLKTEYIDKPIFRHPGQTLNRIKVEKGVDLINTGTMELYSDRLE
                     ITSEDNSRRDVFPLSDIAGFSVTKMMRILFSLKDGSYFEIKSDIPRTGMGYMMLYRYL
                     TGKDYI"
     gene            25137..25334
                     /locus_tag="PLF14_06125"
     CDS             25137..25334
                     /locus_tag="PLF14_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70902.1"
                     /translation="MNEKTDFAVETDKNKKESNLLQIKADDPNKETLLALLESESISQ
                     NPDTIGYTNVEDALRNLKNNL"
     gene            25471..26829
                     /locus_tag="PLF14_06130"
     CDS             25471..26829
                     /locus_tag="PLF14_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT70903.1"
                     /translation="MLSYIRLRNFKSFSDIMFDLRGKGGIPKKKVFVYGENGSGKSNL
                     ISSLFFLRKTVESMRRQTDLKKITDVYLSEEYDKATILNERRGILEDLIKNTLYTSEN
                     LIKEYKTINSKGGIEIEVGFFLGGVDGTYSLSFDDARVVREELRYKLNERTGTVFQLS
                     EGNIRMSKAIFFDDTYSKELKQNIEKYWGMHTFMSILDNETATKNNKFIELRVHKNML
                     DILKWFQGYSVSCREAHLEKIKPGTPFSLFRDLESGYVRIGDDKELFTLEKLLNNYFT
                     SLYSDVKKVYYKFETKEGRLQYKLYLKKLLNGSILDIPFELESTGTRKLLSILPYILS
                     AVSGKTVFIDEIDGGIHDLLMCEVIESLGDALDASQTGQLIATTHNTRIMKQLANDDV
                     YILSIDVDGNKNIFSVDKYDFRTQKTHSIQSKYLNGDYAGVPFTGYLDFSEMVEEIEA
                     CI"
     gene            26957..27949
                     /locus_tag="PLF14_06135"
     CDS             26957..27949
                     /locus_tag="PLF14_06135"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT70904.1"
                     /translation="MKGTILLCSDSSQEGKDFDQFDKLVKDTNDKPAVDGLIFTKIKA
                     IRNVQYYVFVEQMGDNTDFVLNLLALYVKDYLTNNIEKTDKEFFFKQILQNDISGNEL
                     NKRNKVFRLDTGAKKTVVTVRTGNVPEYPVSEILKNLFRDTKNTVITTLDNDDTVVIL
                     AAKEDIEQVLLSAVSTLEEQLMVPVKASIGKYFTLLQDARNSYITSVKAFDIGSLYSE
                     GYSVYNYNNIGIARFFHDIPIEQLEHFIEETITSEVYDRIDDETIKTMQAFFENNLNI
                     SETARKMYLHRNTLVYRIEKFNKLTGLDVQKFESAVLFYTICQIKCFLRNMGKD"
     gene            27951..28646
                     /gene="ftsE"
                     /locus_tag="PLF14_06140"
     CDS             27951..28646
                     /gene="ftsE"
                     /locus_tag="PLF14_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HOT70905.1"
                     /translation="MIDFDNVSMIYKSGTEALHGVTFHIDPGEFVFITGKSGSGKSTI
                     IKLLMREEKPTSGDIFVNGYDLLSLSRYDLPHYRRRLGVVFQDCRLLMNKTVYQNVEF
                     AMEIVGESNRTIKQVVPRLLHLVDLEGKEKSFPDELAGGEQARVALARAIANAPDILI
                     ADEPTGNLDPKTSVEIVDLLYKVNRLGTTVVVVTHDTRIVDRLQQRVIRLQDGEIVSD
                     TEGSIYENEACEY"
     gene            28627..29520
                     /locus_tag="PLF14_06145"
     CDS             28627..29520
                     /locus_tag="PLF14_06145"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOT70906.1"
                     /translation="MKPVSIKYILIESFNNIVRNKVLFITSIFTVCVTILLMGVVGIL
                     LMNVSYNSDTMMEEMLNITVYVIPEADEKTLNDIQDVLVADAHVTEIKYVSQMDAYQA
                     GIELVGEEVMAELGHEFLPASFIVKLDDVTYAHIFVRNMKAMSNIYEVKYSSEAYERA
                     GAISYTIRYVCGVLTALLGILSLFLISNTIKITVSASKEEVTIMRYIGASESRIKAPF
                     YLQGAFIGFLGAIISFIIAGFGYNYMYTRLEGDLSEYMGVLELVPVPQMLLVLLGVFL
                     LYGIGLGILGSFFAIRKYLKA"
     gene            29536..30696
                     /locus_tag="PLF14_06150"
     CDS             29536..30696
                     /locus_tag="PLF14_06150"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOT70907.1"
                     /translation="MMKGTEKLRKCAIILVFVLLLSFTHATFCVGDINEAKDNLGNIQ
                     DQLKKIENEIKQKQSIKSYYESLAKNMKNSISSQEIQLATYNEYIVEVTGKIDSLTET
                     IEQAEQDYAERIKVLKDRVVNAYINSNLSMLDVLMQSSSLKEYYDYIELNKLITKHDN
                     LLIEEMKVMMKDITAKRETMEMLKASYITLSEQAQIVLSNMQSMQSDALETINVTGST
                     IKVLKAKEDALEAEEAEMLNLILKLQREIDYIGGQMTWPLPSWKHIPTGNGLFGWRLH
                     PIFFEWRMHNGVDLGGVMGKNIVAANDGVVSIASWGDGYGYYTAIDHGGGVTTLYAHC
                     SKLLVSAGQSVKKGQVIALIGATGWATGPHLHFEVIIGGKRVDPLLYIDPSR"
     gene            30718..31986
                     /locus_tag="PLF14_06155"
     CDS             30718..31986
                     /locus_tag="PLF14_06155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOT70908.1"
                     /translation="MKKILIKKTITILIMLAVTSFSLLTGCSLIKELTPIDRAIARRI
                     TTVTLPEASPQNVKLEGEFTEEQLEKFNEVYNLLLKMSIYDIDHEKMMEAIIAGMSSV
                     FDAYTMYVPEEHAQSIRESSSGEYKGIGVTIVTPEDGIGAEVVSVNPTGDAYVKGVKP
                     GDIIIKVEDMELTKSMSLEYIASMVKGEEGTYVNITIWRPSEEKEIQFNIMRMLINTI
                     EVTGKMIEGTDIGHVYISSFSADMLDEFVDIFNDLVLKQGMKKLVLDLRDNPGGDFYA
                     AVSLADAFIEEGVITTLADGTGYEEEYTSDSYAVSIPTVILVNENSASASELFSGAMK
                     HYGLATIVGTRTFGKGVAQSIYDLDDGSQLRVTSYQFLLPDDTCIDGIGITPDVEVLP
                     FEGYEDAYVDSLELEHDAQLQKAIEILESK"
     gene            31983..32720
                     /locus_tag="PLF14_06160"
     CDS             31983..32720
                     /locus_tag="PLF14_06160"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT70909.1"
                     /translation="MMYDCIADVYDILMKDVNYAAYADYIDEIIARYQTKKSEIILDV
                     GCGTGSLTCALRDKGYSMIGTDPSQNMLNIALSKDTSNIQYINQSMENLDLFGTVQTA
                     VSLLDCINHITDRKRLQKAFDRVSLFLESEGLFIFDLNTVYKFETVLAKNTYFDLSAD
                     VSYTWMNKYNKKTGLCRMEITYFIKNQQGCYKRYDTCNIERAYSDKEIIDMANRSGFE
                     LADMLDDLSFDKPKKNSTRKFIILKKK"
     gene            32728..33582
                     /gene="hslO"
                     /locus_tag="PLF14_06165"
     CDS             32728..33582
                     /gene="hslO"
                     /locus_tag="PLF14_06165"
                     /inference="COORDINATES: protein motif:HMM:NF001033.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HOT70910.1"
                     /translation="MDRIVTALAYDKKVRVFIALSTNTVNEAIRLHKLNLMSAMALGR
                     LLTATAIMSYMTQSESIAVQVSSEGPMKGVLAISDEKGKVKGYVTNNDYTTYGENITS
                     LGQLLMPGKLMISRDFGLKEPYTGITRLITGEIAEDIARYYMDSEQTPTACALGTYFD
                     DKGKLLSAGGYIIQVLPGTEDRILNEIEQRVSLTGNISKLIYDLETPENLLNFLFSDR
                     NPEITGTHGIAFKCSCSKEKILDKLLTIDKEQLNEVAKQQSTIEAVCPYCNSHYHYHS
                     KDILKIIE"
     gene            33595..>34098
                     /locus_tag="PLF14_06170"
     CDS             33595..>34098
                     /locus_tag="PLF14_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70911.1"
                     /translation="MKNKEKVKRITGVIAAVILFCVAIAILIFLFRKGDIINTPENPT
                     GQVTESSDLSSPTSTRQYTPIEGLNRYLPRKNNPQNVTLTADKDKVINDMFNSTVDND
                     ATVYISFPDSSIACYENDKLNLNTGIFHEKHNYIATVTIISDSIIKSGYLLADRDGTI
                     YMNEIKKS"
BASE COUNT        11017 a         6043 c         7638 g         9400 t
ORIGIN      
        1 tcttgcaaag cgcttaggcg ttccgtgctg tccggcaaca cgtgcagata aagacaccgt
       61 attttccaca tttctatatg atttttcaaa ggagtatatt gttcatttcc gccgtctgtt
      121 taaaggagag cggtcggata acgagtatag aaatcttatt gaagtgcgcc ctaagttcaa
      181 agaagatatt tcacggatga ttcttctgga cttcattaca cggcaggacg acagacatct
      241 ttcaaacatg gctattaaaa ttagcggcaa caaggagagt ttctatcctc tctatgataa
      301 cggcagaagt ctgttttatg aagataccga ggaaacggtc gaaaaagcaa ttaaggatat
      361 tgcaggctat tcaacggttt tcggttattc cggtacctat tgggagcatg tacaggatat
      421 tgctgccaaa ataaatatag cggatttaat taatctgaat gttacaaaag cggaagttga
      481 aatcatactc aaacaggcag gcttcgtttc ctatcgcttg gaaggtgcaa cggaatgggt
      541 cgttaagtca attaatatgc tgaaagaaat aaacaggtag tggagattta cctatctgtt
      601 ctttacatgg tgaagagaaa tacatattga gaaatagagg aaaataagag taattttctt
      661 gaatttatga actttttgta aacaatgccg tgaatttgta aacaatttat gaacaaagca
      721 atttgctaat cttaaaggta tataatagag cacagaagtt acttttagtt aacatataat
      781 atttataatt aataaagaat gtgaggtttg atatgaaaaa gaggttcatt gttcagtttg
      841 tagcaatatt tgtattgctg tctgttttag ttgctccggt gtttacgggg tatgcaaaag
      901 gagacacaca ggattcacaa tcggaagctt cttcggaggt aacggaaatc tcttgggata
      961 acagaaatac aatacatgcc tgtgtctcag tcggagtcat aagaggagag ggaggcctta
     1021 ttgaggatga gtattggtta agaaatacca caagagcaca gggattgttg ataggtttac
     1081 ggttacgcgg attggaagac agtgcgcgat tctataaagg tacatatagt tttaacgata
     1141 tgccgggctt gaataaagca tactggagcc ctattttagc ttatgcatat gagcatccgg
     1201 aattaggcct tgttactgat gtcaagtcag gtttccgacc gtcggaggtt ttgaccggaa
     1261 atgagtttgc aaagataatt atggaggcgt tgggttataa gcaaggcgtg gattttgaaa
     1321 gcgccgatgt aagatccttt gctcaatcaa agggattaac cgtaaaagaa ggtaaagtta
     1381 caaatcagga ttttaccgta gtattacagc aagctttgaa gttgaataat aaagaaggga
     1441 aattattcat agaggattta tatcaaaaag gtgctctgta tgaccgaaga gaggtaaaag
     1501 ctgctcccgg aaccgaccct gctatacctt atgctgccag aatgcggtta ttttactatg
     1561 aaggcggatg gttacaattg cacgccagcg accaatacgg tcagagaata ttacctccga
     1621 aaggcacatt cagcagttcg gacacatcaa aaattgtctt gggcgaccaa agaccgggtg
     1681 ttattgctac aacggatgtc agtgttgatg catacggagt tacttccggt ataaataccg
     1741 cagttgtaac cttcaagtcc gatgacggaa aatggaattt aagtaccgaa gtgactgtgg
     1801 tagggaaaag tgatgtgaat gtttttaccg atatcagata cgagcaggat tatcaggcat
     1861 tgatggtagg aaaaacaatg aaacttagga aattttcttt gatagacagt ctggggaatt
     1921 ttgtaaatct cagctttcat caatattatc ttgaagtgag aagggcggat gacaatgacc
     1981 cttacggaac gggtgattcg gcggtttttg aaataggaag acaggaaata aatgagcact
     2041 atacatatat taccgcaaaa aaagccggaa cggagacact taagattgtt gcctgcaaag
     2101 gcggtatgcc cggctccggt aacccgaatt atatagaagc ggtgaaggag tttactctga
     2161 cagcagtcaa tgatacggat attgtcggat gcaaggtaga tttcttatac gacaagaatt
     2221 tcatgtatgc gggagaatta tccgaagacc ataatctgaa gataaaagtc agcggagcaa
     2281 ctgtaaacag gatgattgtt cagatacagt ctacagatga agtagcgcta ccggatgctt
     2341 ttagttacaa ggttgatgtg gataacattg cggtatcaac tgaaaaaagc gaaattgtga
     2401 cggtaagacc ggttacgaaa gatatggaag ccaaaataaa ggttatagga aagcgcggtt
     2461 atgtaatagg cgtatttccg ttccacttaa aagtcgatgc tccgaaagtt cattacctgt
     2521 atatgtccgc ttttaacaag gattatgtgt atatatatgc ttatgaccaa tacggtaaaa
     2581 gtatagcggt acccgaagga accataacca gttccgatcc ggataaaata gctattaccg
     2641 gtacaagaga aggctataat ccgtatgacc tttgttataa tatatcctct gtaggagacg
     2701 gtccgaacac cgcgacaatt acttatgtta ccgatgacgg actgtttaaa gccgaggtga
     2761 cggttaccag gtaatatttt agggaaaaaa gagtgaaatc cggaataaca aaagttttgt
     2821 ccgtatttta ctgttgcttc cggtaatcac attatgacct tggcccgtct gttataaagt
     2881 tctatgattc cgaaggaaac gcatctgtaa agcaggtttg tgaaggaaca attataagtt
     2941 ttaatgtttt ggattttgca ggcattgagt gattaaacta tttaatattc ctataaaata
     3001 agaggttata actgttattc cacagctata accttactta gtttaacgga tattatgtaa
     3061 gccagtatac aggatactat gtctttcagc aggtagggta atgatactgt aagtgccgtt
     3121 tgtatgaaag gtttacccgt tattatcata aactgaataa tgccgcataa gtgacaggtt
     3181 aaaagaccgg caatgcatat cgctattgca gctattctgt tctttacatt ttttgcagcg
     3241 gcacaaagca acacgaaagg tatgaatccg aatatgaaac cgcccgttac tccgaataaa
     3301 gtaccgaacc cgcttcttaa gccggagaat accggaatac ctattgtgcc tattgtgatg
     3361 tacaggcata cggacaaggt acccgcctta atacctaaca gtaagcccgt tatagctatg
     3421 gccagagtct gcaatgtagc cggaacgcct gaaggcatag gtattgccag ttgggacata
     3481 accgctatta ttgcggtgaa tattcccgta tatgctattt gttttgttcg cgtcatcatt
     3541 ttattcatta tacactatct tggggtgctt agtgtgtaaa tatgcctgat attatgataa
     3601 aatcaatttc tgacagggag ggtgcaattg tccagagtat gtcaaaactg caggttgcga
     3661 gtttcggata aagccaaata ttgcagaaaa tgcggatctc ccataggtat tgccaatgta
     3721 cctgagtatt cggcagccga aaacatgatg cagttcagca agcctttaga gaaaagcaga
     3781 aacaggctgg ggcttacggg cttaaagtat atgcttatcg atttacgaaa gggcttgaga
     3841 agaactactt atcttatccc tgtatttgca gtagcagtca tatggattgc ggtaagtctt
     3901 ctcttctctg cagggattga tacggttttt ttaaggatgc tttccttttt tacttatgcc
     3961 gatgccggga tgaacggagg attcctgggg cttacaggag gtattttagg caaaatggca
     4021 tatgcatatt ttattgtaat gctgctgaca cctctgtatt cgttgcagaa accttttaag
     4081 ggtgtaattc cgggggcaaa aacatatatc tctcagtctt ttcgttttaa aacactgaag
     4141 gatgtatatg tgtttattct cggcatgggt ttttccttta tggcatacaa catgctgacc
     4201 ggagataatg cgtggacaaa aagcattatt tccttaataa ttgcggttat atttttcaga
     4261 ggtatgggta aaaagtacgg ctttacttgg gatgttttcc acagggtatt ttccgattcc
     4321 aaggatgagg atataacggt gaaggcaatg gggggatatg ttaccggtct tttgctggcc
     4381 gttcctacct ttttgtttga ttcttcattt ctgtcatact atatcggtat tgtgctgata
     4441 gtgatatttc ttgtactgaa gtttaccgaa tttatcatag tccacaaagc cgattttaag
     4501 cggatattcc cgattatcct tgtttgtttg atactgtttt atccgttaag tgctttatcc
     4561 gcaccggcaa caagcgagat aagaggcgta tggatattgc aggatgtcaa gttggttaat
     4621 cccgatacca aaaaaaatcc taaagacaac acatatgtat cggacggata tatacggaaa
     4681 caatattccg cggattcaca atataccatt gaatactttt ggaagagtct gccgaaaaaa
     4741 atatctgccg gagaagaatc aagagtaatg atagaaacga agttggttta tacatcggct
     4801 gaggaaacgg cactgaaaac acatttgaac tcggctatgt tcataacgta taaacccaat
     4861 cccaaagaac cggaagttaa taagtatttt cccggaagcc gtgatttttc cgttgaagga
     4921 gttttttccg agcttcagca ggagcatacc gcttttttcc tgaaaatcac cgattattac
     4981 gaactctatg acttgctgac caagaacaac tgccacttaa ccgtttcggt acaggcttca
     5041 ttcggaggca gggtggagta tatatacaga cttcaacgaa cgcctgttga cggttttctt
     5101 acatggttgg cgggtgacgg gaccgcatat gatcatttcg gacctttagg aacggttttt
     5161 tccaatgtag catcggttac cgcatccatg ttgtgtgccg ccgtagcggt aggatcactt
     5221 acgttgaaga gaaagaaagg tttactgacc gatataaaga gaacggataa gaaagaggag
     5281 atacctgaag agaaatatac cgacatccgg accgagagtg attttgccga taatcttgta
     5341 aatctctatg aaacattttc caatgctttt gcagccggat ggcccaaata cagagaggta
     5401 cttataaaga ccctttcaag aagtaaagat aatgcggttg catacaaaag tgtcggtgca
     5461 atagaatcct caataaacaa ctacttcaac agtctgtccg gcattaccaa cagtctggca
     5521 gctttggggt acaacaatga ttcacttggt ctgcagcagc ggggcgaaga gattaccgat
     5581 gccacaggca agatagtata tgcaaactat gtcttccaga gtcttgcatc gggtacatcg
     5641 tacagtgcat ttatggagct tgcaaacgca tttgctttgg aaaacaccaa agcgggagta
     5701 attgcctctc cttcaataca tttaagagga ataatgtctc ttgttttgga ttcgataagc
     5761 gatgcgcaaa atgcatacaa aaacgccgtt aacggaagat acggagaaaa gataaggagc
     5821 atggtaaaag acggcgaact tacttatgaa tctattcagg catttgccga taccgtaatc
     5881 aaagccgatg aaacggacag gttttatgaa agaatgctga ccggtaccgt tatgcctgaa
     5941 gaccggaaat atctttcgag ctatacggcc tgtgccaccg aaagggcggc taaacaggct
     6001 caaggtgccg ctttcagaca tgaaaataca tcactggttg tcagcagtct gtttgactgg
     6061 caggaggagg aataatgttc tatatcttaa gtgcacatga ctattcggcc cttatagccc
     6121 tatctgatat aaagcagaac tatatctcgc aggcggtata taatccggac ataaacagta
     6181 agacctcttt tacgcgggca aaaagcgaat tatcgaaatt ggggttggtg aaaaagggag
     6241 taataacaac taccggccga gtcattatgg aggccgtgtt ggaccccgag agggttgtgt
     6301 cggtggtaag ctgcaactcc atagatatgg caactgccca ttactgtttt tcaaaaggat
     6361 tctggttgcg actgttgccc aaaaacgatc acagcataat tacaatagag tcacccatag
     6421 aggaaaagga tcttgtgcaa aagataagga gagactttct gcaggatttc gactttaccg
     6481 atactcagcc tatcgatgtc tttttaacgg taaaagaggt ggttattttc cagcttatgc
     6541 agttggccat agtggataaa gtcaggaaaa aaggtgcacc tttaacacgt gccgccgctg
     6601 cattgggtat ttccgagttt ataaccaata aaaatgtttt aaatgcggcc gctgctgcat
     6661 atatgctggg cgaaaaggcg ggttatgaac tcagcaggca gttttgggat acgaataatg
     6721 tcatatccca tatagatgca ttggtcggga aaaatatatt cgataaagtg attgaacaag
     6781 gtacgggaaa gttgaagtat attatgagcg atatttcaag aaagtggctt actttagact
     6841 gccttgtgga taaaataacc attcagaaac ttccgcaggg agatataatc aatgtgagta
     6901 taacaaagtc gggaatgatg acagtaacac aaaatgcgga aggattacgg tttaagacca
     6961 taacaattaa tgaattttac gaggagaaat aggatgaaaa agtatgttag agattcatat
     7021 accgaacagg cacagatatt aacccagtcc gcattaaacg gcaatgacag actgttcgga
     7081 ggacgtatta tggaatggat tgacataacc gccgcaatag ccgcaagaag acattccaac
     7141 cgcaatgtta ctacggtggc ggtggattcg ctggaattta aaggaccggc ttatgccaac
     7201 gatacggtac tgcttacggc aaaggttaca tatgtcggga aaacctccat ggaagtatgc
     7261 gtcaagtcgt atgtggagga gttgaacgga gaaaagaaac tcataaatgt agcatatgtc
     7321 gtaatggtgg cattggatga gaatgaaaga ccggtggaag ttcccggtct gattttggaa
     7381 accgaagaag agcgatgcga ctgggaaatg ggtaaaaagc gatatctctt acgtaaacaa
     7441 cggaaaagca acgcaggctg ttaaagtatt ctgacggatt ttaagttgac aaattggctt
     7501 ttcagaacta agaaagcccg taaacatggt atatatttaa gttgacggta agttttacgg
     7561 gagttgtata tatatgcgat tgtgatataa tttatcggat tggacgggag actttttaag
     7621 tgcagggaat aactatagga caatacatac cgggagattc aattatacat aaggccgatc
     7681 cgcgccttaa aataatcatg accttcttca ccatgattct cttactcata attgactctt
     7741 tttatccgct gcttttaagt gcggtgtttg tcgtaactgc ttatcttgtt tcgggaatca
     7801 gtttacggta tgcaataaaa ggactcaaac ctgtcatata tattgtcata tttaccatga
     7861 tattgaatat tttctttgcg accggtactc cgttatttac tctcggattt atcacagtta
     7921 ctaaagaagg acttgtgaca tcggctttgg taagtgcaag aatattgttg ttgatttccg
     7981 cagggtctct tatgacatac accacgacac ctttggtgtt tacagtgggt atagagaagc
     8041 tgatgtatcc tttgaaatat cttaaagttc ccatagcgga aatagccatg atgatatcca
     8101 ttgcattgag gttcatacct actctcatgg aagagaccga gaagataatt aaggctcagg
     8161 cagccagagg agcggatata ggtacgggga atataattgc gagaaccaag agtttcatac
     8221 cggttttggt gccccttttc atatcttctt tcaagagagc ggacgatctg gcgacagcca
     8281 tggatgcccg ctgctataac ggttcggagg ggcgtacaag tataagcgaa ttgaagtacg
     8341 gaaagattga ccttgccata tttatggtat atattttgtt tgttgcagcc gctttggtgc
     8401 tgcaatatgt gttataggag ggaagacatg tacagtatcg gaattgacct cggaggaacc
     8461 gcaatcaagg cgggtatagt agacaaaagc ggtaatataa ttgttaagga caaggtaccg
     8521 acaaaaagcg aaagaggcta cgaagccgta ttgagagata tggccaaact ctgcaaaaag
     8581 cttatcgaaa aagccgggat gagtgaaaac gacatagaaa aaataggggt gggatgccct
     8641 ggaactcccg ataaggtaaa cggcatactg atatataaca ataatctcgg aatgagaaat
     8701 gtgaatataa ggaaagagct gcagaagtat ttttctcttc ccgtatacat cgacaatgat
     8761 gccaatgttg cggcgcttgc ggaaagtgtc tgcggagcgg ttaaaggtac atactgttcg
     8821 gttaccataa cattaggtac cggtataggc agcggagtgg ttatcgacaa taaggtttac
     8881 agcggattca attatgccgc agcggaaata ggacatcaca ctctggtaat ggacggcgaa
     8941 ccctgcacat gcggaagaaa cggatgctat gaggcttatg cttcggctac cgctctcatt
     9001 gcacagacaa aaaaagcggc acttgcaaac agagattcca agatatggga agcatgcggg
     9061 cacaatttgg ataatataag tgccagaacc gcttttgaag ccgaacgaat gggagattcg
     9121 gtggcaaaga atgtttgcga cacctatata aggtatatcg gagaagggct ggtaaatata
     9181 ataaacatat tccagccgga ggttatagct ttaggcggag gagtatgcaa cgaaggcgac
     9241 aggttgctta tcccgttgag agaatatata ttgaaataca aatatacgag ggaggagaca
     9301 ccttcatcaa cattggttat agcgcaaatg ggaaatgatg cgggtgtggt aggtgcggca
     9361 atgctcaccg aataaatatt ttatgaaaaa acattattta ggcaatgtaa aaagtaacga
     9421 agcgactatt gacacgtatt tccgtatata tgatcccgct attcgtgcat tcaggaattc
     9481 ttacggatat gcttcacacg caatgcacac tacacccatg gactctttgg cggcagggta
     9541 ttactgtaaa gacaataagt actacaaaga cccgaaactt ctggaactga tgcataatgt
     9601 gcttgctttt atggaaagcc atcacaggcc caacttcaca atagatatga tggaaagcaa
     9661 cttcttttgt tcgcctttat ttgaggccgt aaatatatcc agagcataca gaataatgca
     9721 aaagtatgca tcgaccgatg atgaaaagaa actgctaaaa agatttaccg actatcttcc
     9781 gatgatggaa aaatccttgt tgaacggagg aattcatacg cccaatcaca gatggatagg
     9841 agcatcggga atgcttatgc tggcaaacat aatggacagt acagacttaa gaaacttagc
     9901 tttcgcctat ctgaacgaag gtgtggatat gaatgagttc ggcgaatata ccgagcgttc
     9961 tccgcattac agcgaagtat gtaacacctc tttcatgcag atagctttgg aaaccggtga
    10021 taggagctat cttgaatatg ccaagaaaaa catggatcta ttattccgtt atctggaacc
    10081 taattttacc atgttcaccc aaaactccaa cagagtggat acgcatgaag gcaatgttaa
    10141 tgccgttaca aaaccgtact atctttacta tacatatctg catatgtcct atctgacaaa
    10201 taacccgcag tatgccaagt ttgcagacga tatatacaat gccgttgaaa agatacacgg
    10261 aacgggtccc ggatcactgt ggatgtatct tgcatatccc gagcttatgg aatatgagcc
    10321 gaagatggag cagtatccca cttcatacaa tatatttcac ggtgatatag tgcgatacag
    10381 aaacgaagat ttttccgcat ctattcttac aagatcgccc aacttccttt tcgtgcagac
    10441 aggtgatttg agatgttttg taaggatatg tgcttctttc tttgccgtgg ctcaattcaa
    10501 gcctttgtca atagagctta tcggtaagga tacatatcgg atgaagatga aagctcacgg
    10561 cgagtattac ggacctctcc cggaaaagcc tgcaactacc gcatggaaag aaatggatca
    10621 ttccttaaga ccggttaata acaaggtgga tttggaatat atagttacgg taaagattaa
    10681 acccgacaga gtgagtctga atgtaaaaac ggaaggatgc gataatgttc cgtgcaagat
    10741 agagttctgc ataactcctc ctgtagatgt gcaatatgat tctgtggaac ttaaaggact
    10801 gcccgatgag tgtttatcgg taaacgcaaa agagattaag attaaaaaag agaatgattg
    10861 tcttacgctt acaaacagtt tttccgaaca cacatatcat aaagatatga gaggttcggt
    10921 acctcccagc aagggcgtat tcactgttta ttatacaagt tttacgcata tagatgcgtc
    10981 tgttgacata ataggcacga aggagacaaa atgaaatcgc actacccgga tatagtcaaa
    11041 ggtaatgagg cggctatcga gaatgactta aaacgcttta atcccgatga gcgtatcata
    11101 tacggcgctt acggttttcc cgactatgcc aataatgcgg tacctataga gaccatgtct
    11161 tccggctatt actgcaaaga cagtaagttt taccgtgaca aaaatttgtt gtatataatg
    11221 gactgtctca tagactacct gtattccgct cagcgtgaag actacactgt ggacaccaag
    11281 ataagcgatt acaaaacggg acctgtactc ttatcgaatt ttcttgtaag aggatacaga
    11341 atcatgaaga aatatgctaa gaccgaagaa gagattgctc ttagggacag actgaaagct
    11401 tatcttgttc cggtagcaaa aggcgtatgt aacggaggta ctattacacc caaccacaga
    11461 tggatgtatt cctcatcggc gcttatgctg tacaacgagg ttaagatgga ctgccttaaa
    11521 gaaatttcgg aggcatattt aaatgagggt gtggatatag atgaggcggg agagtatacc
    11581 gaaagatctc cgatgtacaa cagagagtgc aataacgctt ttattatcat tgccgaagaa
    11641 acaaaaaatg actattatct ggaaagcgtc agaaaaaatc tggaactgat gttgtactac
    11701 atagagccgg actttaccat gtttacacaa aactccaaca gaaaagacaa gcaggagggg
    11761 gatgtgagcg caacctttta tcccgacgga tattatcatt catacctgca catgtcttat
    11821 ctggataaga atcctttatt tgccggaatg gccgatgaga tttttaacag tataaaagaa
    11881 aggacaggca ggggaccttc ggttctgtgg atgtatttac tcaatccgaa gcttaaagag
    11941 tatgagccga aaaccgttac tcgtcccgcc tcttttaaca tattccataa ggatatagta
    12001 agaagaaaga atgaggactt ttccgtaact ttacttacca gatctcccaa tttccttttt
    12061 gtacagaaag gcaatttgcg gtgtttcgta agagtatgtg catccttctt tgcggtggca
    12121 cagcttattc cccaaaaaat agagaaaacg ggtgatgaca cttaccgttt tacaatgacc
    12181 gccgagggca aatatcacgg acctctgccg gagaaaccga acacagttgt atggaaagaa
    12241 atggaccact ccaaaagacc tgttataaac agagtacaat tgacttatca tgttacaatt
    12301 gctcttaaag atacaggtgt ttgtatggat ataaaatccg aaggcatgga ccaggttccg
    12361 tataaggtgg agttctgctt taccgctccc tgtaccgtaa agaacaaaga caaacggtat
    12421 gattgccggc cgggagaaca tatgaccttc aatgaaggcg acattgacat tgaaaaagac
    12481 ggtaacatac tgactataac aaatactcac aacagccata tttatcatga cattatgaga
    12541 ggttcggtac ctaagagtgc aagctctttt acaatatatt acacgggatt tacgcatata
    12601 aatgaacata ttgaaatatt gaatcaaagg agagaaaatg cctgacatat tgacacatat
    12661 actttttgca aagaatgtta aagatgccat cggcgatatt aatgtcaaag acgccataaa
    12721 gaataatatg cagttgtaca atttcggtgc acagggaccg gacttcctct attatcacat
    12781 aactccgctc tttttcgata acagagtacc cgcagcggga tcaatgatgc acaggattga
    12841 aacggctaaa ttctttaaag actttgtatt gtggttgcag aatctggacg gttccgctta
    12901 tcagcaggga ctggcatatt ttgccggttt ccttacccat ttctattgtg ataagactct
    12961 ccatccttat gtgttggcga cagtaaaaca aggggcaaac tattttaact caagcaccgg
    13021 taaagtgtat ctgtctcact acatggtgga atatattatg gatataaggt tgtggagaga
    13081 aaagatcggt atagatgcat acaaacagga tataacggaa cttataggga cggagccgct
    13141 gccgtatgaa atagtggagt atataaccga gttcataaac tacacacaac ccaatgcggt
    13201 gacaaaaaag gaagtataca actcggcagt aaaaatgata cggacacaaa aagtattcta
    13261 tgatcccaag aacataaaga aatggtggat taatctttta ccgcttccga agaaatgtta
    13321 tgtggaaaag gtaaaagagg atgtggatgt attgaatgtg aacggacgca tctggagtca
    13381 tgttatgaat gaggatgaga agtccgattc ctctgtatac gatcttatgg aaatagggtg
    13441 taatgaatgt gtcaaggtgc tggatgagat aagcgaaatg ctggaaagag gaataggcaa
    13501 agacctcaat aaacttatac ccgatgtagg ttatctgaca aataagcctg tataattcat
    13561 ataaattgta tatacaaata gcggacggga gaaggagaat gcctcaggca acagttagtt
    13621 tcagaataga cagagagccg gaaagacgtg tggcaaatct taatgccggt atatccgttc
    13681 ttaaagcaca tgaacttata gaggttgact gatgcttaaa atttgacaag cataagaccg
    13741 attatcgtag tatatggtgt ggagtatgat cacttttgca aatagttttt acgaaaagtt
    13801 tagtcagtaa gtgcatctcc attaataaga caagaagtga ggtaatcatg aaaacaacta
    13861 tgaaagagag tgaaaagctt taccgtaaaa ctatcattaa ttttattatt ctgtttgttg
    13921 cgtgtcagat tgctttttta attgtactat ccattgttta cggaattttc ttaaaatatc
    13981 cttttttgtg ccgaatagaa aatgatgggg gagtgacaat tactaagtat ttcgctaagg
    14041 aaaaaaaagt ggatataccc gaatatatca caggaaaacc tgtaaccggt ataggcttca
    14101 aagtattttt agataataaa aaattgactg aaattatttt accttcaacg cttaaatgga
    14161 taggaggtga agcatttagc ggttgttggt gtattaagaa tattgatttg cccgataacc
    14221 ttatagccat aggagatgaa gcatttagag gttgttcgga tattaagaat attaatttac
    14281 ccgataatct tgaaagtata ggttccagag catttaaaga ttgtagattt gattatataa
    14341 aaataccgaa gagtgtagtt cgtatgggct ccggtgtgtt ttccggttcc tctcttaaaa
    14401 cagttgaatt gtcgtccctg cttccggaac atactttttc cggatgtacg gaattgcagc
    14461 atgttattat acctgatggt atagaacgcc ttgaggagta cacattttcc ggttgttctt
    14521 cgcttgataa tgtggttatt cctgaaagtg taattgcgat agactggcgt tgcttttcca
    14581 attgtaccaa tctaaaaaag atgattatac ctgaaaagat agaaaccata ccgtatgcgt
    14641 gttttgcaga ttgtgttaat cttaccgacc ttactatttc cgatggagtt ttgagaattt
    14701 gcggaaatgc ctttgaagga tgtatcggtc ttttggaagt ggaaataccg aacagtgtaa
    14761 catatattgg tgaaagggct ttttcagggt gtaagaatct gaagaaaatt acaatattac
    14821 aaaatgttac tgaaatatcc catggtgctt ttgacgggtg tgataacctc ataatctact
    14881 gtcatagcaa ctcatatgct gaaaaatatg caataattaa agggatttct tatgtattaa
    14941 ttgataaata gcagttcgag ttgaaaaatg acatattgaa aaaacgctcg aaataacata
    15001 taatttactt gaacatacaa aagagaaggc ggttatttta aatggctgta aagaacactt
    15061 acaacaacga aagtattaaa tccctcaaag gtccggacag agtcagaaaa tgcgcgcctg
    15121 taatatttgg ttcagacggt ttggaaggct gccagcattc ggtatttgaa atagtttcca
    15181 actccgttga tgaagcaaga gcaggtcacg gaaatctaat tgaggtcata aggcatgcgg
    15241 acaaatccgt aacggtcaaa gattacggaa gaggtatgcc tgtcgatttt aacaagaatg
    15301 aaggaagata taactgggat cttatctact gtgagctgta tgccggagcc aaatatgacg
    15361 gtaatctgtc gggcaactac aaagcatcaa taggtttgaa cgggctgggt tgctgtgcca
    15421 cacagtatgc atcggagtat atggatgtgg aagttatacg cgacggattc aagtacaatc
    15481 tgcactttga agaaggatat aataaaggcg gactcaagaa aaaggaagat aagagcagaa
    15541 aaaccggtac aactacaaga tggaagcccg atcccaccgt ttttaccgac atagacatac
    15601 cgttggaata cttccaggat atgctcaaaa gacaggctgt tgcaaatgcc gggcttaagt
    15661 ttacacttaa ggatgagcag tcggggcaga cttttgaata tgtttatgag aacgggataa
    15721 gacagtatct ggaggaagtg gttgcagata agaattgtac aactcccgta tttatctccg
    15781 aagacacgaa acttgtggaa aaagacggtt ccagagaaca tgatgcgaga tttaccgcat
    15841 gtttctgttt ttcctccgac atgagcctta tagagcatta ttacaattcc agctatctgg
    15901 taaacggcgg atctcccgaa agagctatga gagccgcttt tgcctatgca ttggataaat
    15961 ttattactac cgaggggaaa tatacaagag gtgaagccaa gataacatac accgatattc
    16021 aggacagttt agttttcata tccaacagtt tttcttcggt cggaggattt gagaatcaga
    16081 ctaaaaagtg tctggttaat tcaatattcc aggatgcctt tacggagttt ttaaagcatg
    16141 aactggaagt gtattttata gagaacaagt cggaagccgt caagatagcc gatcaggttc
    16201 ttttaaataa aagaagcgca gagcaggcgg agaagaccaa gcttaatctg aaaaagaaat
    16261 tgggtaaagg tctggacatg aacaaccgtg tcaagaaatt cgtcgattgc agaagcaaag
    16321 atgccgataa aagagagata tatatagtgg aaggtgattc cgctttaggt tcggcaaaga
    16381 tgggaagaga cgcggaattt caggctataa taccggtaag aggaaaaata ctcaactgct
    16441 taaaagcaga atacaacagg atattcgaca gcgatattat tatagacctg cttaaggtat
    16501 tgggttgcgg ggtggagatt aagacaaaag ccaacaagaa gataagtcag tttgatatga
    16561 aaaacctcaa gtggtcaaag ataataatct gcaccgatgc cgatgtggac ggtttccaga
    16621 taagaaccct tatactgacc atgatataca gactgatgcc ttccctgata gatgcgggca
    16681 aggtatacat tgcggaatct ccgcttttcg agataacgca taagaaagat acatactttg
    16741 catacagtga ggcggagaag aacgagatta tagaagaaat aaaaggcaat gaaaagaaaa
    16801 acggacaatc tcccaggatt accatacaaa ggtccaaagg attgggagaa aatgaaccgg
    16861 atatgatgtg gcttaccaca atgaatcctg ccacaagaag gcttataaaa gtaatgccta
    16921 ccgatgtcaa ggatatggat tatatgttcg acttgctgtt aggagataac ttacagggaa
    16981 ggaaggatta tattcaggaa tacgggtata aatataccga tatgctggat gtatcataat
    17041 acatgaaaaa aggtaaaaaa gataataata acaatgtaaa tcaccctaag ccccaactca
    17101 taaccgacat actggaaacc aatttcatgc cgtatgccat gagcgtaata gtttcgcgtg
    17161 ccattccgga aatagacgga ttgaaacctt cacacaggaa actgctatac acaatgtaca
    17221 aaatggggct tttgaaaggc gacagaacca agtctgcaaa tgtggtcggt cagaccatga
    17281 agttgaatcc gcacggtgac caggccatat acgagaccat ggtcagaatg acaagaggga
    17341 atgaagcgct tcttcacccg ttcatagatt ccaaaggtaa cttcggaaaa cagtactcca
    17401 gggatatgag ttatgccgca agcaggtata cggaagtcaa actggctccc ataagtgcgg
    17461 agatgttccg cgacatagac aaagactgcg tgccgtttac acccaactat gacggaacgc
    17521 ttatggaacc ggtcatgtta ccgtcggcat ttcccaatat tttagtaaac gcaagccagg
    17581 gtattgcggt aggtattgca agtaatctgc cttctttcaa tcttgcggaa gtctgtgatt
    17641 gtataattgc atatctgaaa gacagagata tttatttagg ggattatctc aagtgtctgg
    17701 atttctccac gggcggctat gccatatacg ataaagcgga gatggacaat atattcacaa
    17761 acgggcgcgg gcggatacga ttaagagcaa aatacaacac cgacaaaaaa ggcactcata
    17821 tagagatagt ggaaataccc tatacaacat cggcggaagc cattattgaa agtataatag
    17881 cactggttaa gagcagtaaa attaaagaga ttaccgatgt ccgtgacgag accgacatat
    17941 cgggacttaa gataaccatt gatgtaaaga gaggtaccga tatagaacga ctaatggcaa
    18001 tattatatgc caaaacacct ttggaagacg gtttttcctg taacaacaat ttacttatcg
    18061 aagggaaacc gttgcttttg ggaataaggg atattataga ttactggata atgttccgtg
    18121 tggaatgcat cagaagacag ttacgatatg atttagccaa agacagagag cggttgcatc
    18181 ttttactggg actggaaagg gtgctgttgg atattgacga agccatccgt ataatacgta
    18241 ataccgaaat ggaaagcgag gttgttccaa acttgatgga agctttcaga atagataacc
    18301 ttcaggcgga gtttatagcg gagataaagt tgcgtcatct caacagagag tacatattaa
    18361 agagactgga tgacattaaa gatttgaata aggaaataaa agaagcggaa gaaacactga
    18421 agagcaggac caaaatagag aatattattt ccaaacaact tacggaaata aaagaaaaat
    18481 acggtcaacc gagaaaaacc gagattatat acgaagaaca gatagtggaa gaattcgaag
    18541 agccggaatt tgaggattat cccgtaaaaa tattcttcac caaagacaac tacttgaaga
    18601 agatacgtga ggtcgacttc aagacggatg ttgaaaacaa gttcaagagt gatgatgttc
    18661 tgttgcagga attttcctct aataacggtg ctgaacttat aatgttctcc aatcagggta
    18721 atgtatataa gtccatgttg catcggattg atgactgcaa gatgtcggat ttgggggaat
    18781 atcttcccaa tatctgcgag atggaaaaag atgagcgcat aatatatgtt gtcgcaaccg
    18841 ccgatttcaa aggccatatg atttatcttt ttgaaaacgg caaaggtgcc agagtgccga
    18901 ttgaaacatt ccgcacaaaa cagaacagaa agaagatgct caatgcatac tatatgggaa
    18961 gcaagcttct ttatcttgct cttattgatg aaaaagacca atcgtcatat gtggcgataa
    19021 catccgacca aagggcattg atattcagtg ctgccgatat atcggtaaaa gaaaagcggg
    19081 atactcaggg aattacactg attaaactta aacccgatac aaaggtaacg gagatgcttc
    19141 ctctggaaat ggcacgttta aaggatacgg acagatataa gcctgccaat gttcccgcag
    19201 tggggaaagt attatccgac aaggataaaa aaagaaaaca gatttcgtta ttcgattagg
    19261 agagcgcaat gaaaaagata agaaaagcca ttattccggc agcgggactg ggaaccaggt
    19321 ttctgcctgc cactaaagca acacctaaag aaatgcttcc ggttgtggac acgccggcaa
    19381 tacagtacat agtggaagaa gctgcgcaat cgggaatcca agacatagtt ataatctccg
    19441 gcagaggcaa aagggctata gaagatcatt tcgataaatc ctacgaactg gaagatgtgc
    19501 tgctgaagca taacaagatt gccgaattgg aagacattaa aagtatatcc gacatggcga
    19561 acatatacta tgtgagacaa aaagaggcca aaggactggg gcatgcaata cattgcgcca
    19621 agacattcat aggcaacgaa ccgtttgctg tgcttttggg tgatgacctt gtatactcgg
    19681 aaaaaccctg tttaagccaa cttataaagg catatgaaca gtatcaggca tccatagtgg
    19741 gagtgcagtc tgtcgataaa gataaagtaa gtttgtacgg aataattgac ggagaagaaa
    19801 tagcacccaa agttatgaaa gtaaaagata tggtggaaaa gcccgacaaa gcagcggcac
    19861 cttccaatgt cgctattttg ggaagatata tactggagcc ggagattttc gatatattgg
    19921 agcatcagaa accgggtaag aacggggaaa tacagcttac cgatgcacta aggactcttt
    19981 taactataca ggatatatac tcatatgagt ttgacggcat tagatatgac ctgggagata
    20041 aactggggtt tgtacaggct acgatagaat atgctttaag aagagacgat ctgaaagaca
    20101 accttaaagc ttatcttaag gatatatcaa aaaagctgtg attgatgctc tgttttaatc
    20161 ggaataacag acgaataaaa tatgcaaatt taaagaagca aaaaagaaat aatagaatta
    20221 tttcaataac ccgtacagcg catcggtatt gaaagtgtat gctacactgt ccatgtataa
    20281 aagagtggta atgtcatctg catgtatact ttttgcaaat gtatctattg tcatgttgtt
    20341 aagatattcc cggtcataaa accttgtatc cattatgtat accttctcat aatgcggcag
    20401 aaggaagtgt acaaaagagg caccgtagga atcgcatatg acaagtattg ttctgccgtt
    20461 tttgttttcc gttgtgaact cgtgatatac gccgtttcct ccgctcatga acatgttgta
    20521 gtattgatta ttcggattga cgtaggctaa gtcgttaagt cttttttcta tcggtcctgt
    20581 tttcttatgc ataatgtatt tgtattcgac taagggataa tatgcgataa gcgtatcggg
    20641 gttattcagc actctttcgt cctgattggt ttttcggtat gcactgccta tgaatccttc
    20701 gattacctcc ctatcaacat aactctcaag aggtactata tcttgctctt ttcccattgc
    20761 ttgcataagt gcggtgtagg catagtatgc acctaaatgt gtccagtggt gatcggtttt
    20821 ataatatata tactctttac agtgatgaaa aagagcattg tatgcatcca catctatcac
    20881 cgagtcgttt gtatattgtg cgatatcttc caccatttgc ttctgtgacc cggttatata
    20941 ttgcatgtcc gagagattga aatattccat tgcggtcggt attaccatgg aatagacatt
    21001 cacatgttct cccatggaat ctttaagttc attaatgaac attgcatatt tctttaaact
    21061 ctcatctttt gcccaaattg tagccacaac gttattgtcc acaaggaaaa aagggctctt
    21121 caacacttca attgcagaag ctgcccggta ctcaggtttc ggtgtcggtg agggggtagg
    21181 taacacggaa gctgtcggag acggaggagc ttcggtttgt gatgtggcgg gagtgcaggt
    21241 gtttgcagga gatggagaag gtgaataagt gacagtattt ataggaagtg cggaaggtga
    21301 aggagtattt acaggtttac ttatttctat cttcatattc ttcttaaatc cgaaaaactt
    21361 atccgcagct cttctgtatt gtataagagt gtctctttgt atgaaagtgt cggaatagta
    21421 agcggaatag ttgtttgcat atgatttgtc aaaaaaagaa gatacggaaa ataccggttt
    21481 ctcctgaagt ttacggttct ccgccaaaga tacagaaggt ttattcggat tgacaatgtt
    21541 tattatgcct gctgcgaaaa taactataat gaataacagt gaacctatgc aaagtttttt
    21601 catatcagaa attgaaatac agaaaaggtg tatatgtgtc tcctactaag aatattattg
    21661 aaagaataag cagtgccgca ttgaacagcg gaatcattat tccgttcagt atatcggtat
    21721 gtcctttgga aagcctgtat tccaatttct ccgcctgttg tttcaagaat ttccccaacg
    21781 gaacggtgca gatgacagcc gtgagtataa acagtacatt gttgttgaac agaatggtta
    21841 atatggggtc ggaaaatgat gcggctttta tgccgaacat tacaccaatg tagcgtacgg
    21901 cttgggataa atccgtgtgg tagaataaaa cccatcctat aagcattaca agaaggaagt
    21961 atatatgaga gaatatcccg ggaatctttt caaacagttt gataagaaaa gctttttcaa
    22021 ttaaaagcag tacaaagaaa tacagtcccc atatcacaaa gttccagctt gctccgtgcc
    22081 aaagaccggt cagaaaccag actaccagaa gatttcttat tctgtatttt ctgttaccgc
    22141 caaggggtat gtatacatag tcccgaaaaa aacctcccag tgttatatgc catcttctcc
    22201 agaactccgt agcgctttta gatatatacg ggtaattgaa gttttcgttt acggaaaagc
    22261 cgaacatgga ggccaatcct atagccatat cggaataacc ggaaaaatca aagtatatct
    22321 gtaacgagaa cagaagtatt cctatccatg cgccttgtac cgacagtctg gaataatcgg
    22381 tgcctgtgaa agaagatatc aaagtgcccg cagtgttggc tataactgct ttcttggcca
    22441 accctataat gaatctgtgc actccgtaat tgaatttttc ccaggtttct ctgtcgcttc
    22501 ttatatccgc tacccagtcg ggatatcttc ttataggtcc cgatgtcacg gtaaaaaata
    22561 ccgatatgta cataagcaag tccaaaaagt ttctttgggc ctcggttttc tttttgtaaa
    22621 catccgcaag ataacttatg catgtgaaag tatagaaaga caagcccaaa ggtaaggcaa
    22681 tgtcgttgaa aggattttta aatttgaata ttgccgataa gttttgtgca ataaatcccg
    22741 aatacttgaa gaataccagt accgctataa ttaccgttat acctgttata aggtaaattt
    22801 ttgctgagcg cgtgccggct ttttgctgta taaggagggc tacaatgtat acgaaagccg
    22861 aagtcagcag taaaagcagt atatataccg gttctcccca tgcataaaat actaaagaca
    22921 tgacggtaag aacgatattt ctatatgttc ggtttttgga aatgtagtaa agtatgaggc
    22981 ttacgggcag gaaaaccgtt aaaaagaaaa ggccggaaaa aaccatcaga gcacctcctt
    23041 ccatccggaa atattggcat gcgtgaatga ccggaaaagg ttgtatttaa agttgggatt
    23101 gatggaactt tcgttttcaa tatattcctt tattctctga cgatgggtat tgcctgctgc
    23161 gggacacagt gttttaaccg gtgtaatacc cgcactttca ttaaaagccg ttatgtcttg
    23221 ttcaaaagaa tatatcagcg gtcttatcac cgttatatct tttcttgaaa gataggtaac
    23281 gggagcaaaa gtatgcagtc ttccttcata ttgtaaacat aaaataaaag tttctatggc
    23341 atcatctttg ttgtgaccga gagccacctt attgcaacct aatgtttttg ccgtatcatt
    23401 caacatacct ctgcgtaaat tggcacataa agagcaggga cttttttctt tccgttcttc
    23461 aaaaacaatc tgttctatgt cggtctgctt tactaccaat tccacatcaa tgttatcgca
    23521 gaatttttta agataatcgg tatcgggatt ttcttcgctg agttttaagg taattgcttt
    23581 tatatcaaac gattgctcta tatgtcctct gattttgctt aatccgtaaa gcagagcaag
    23641 actgtctttt cctcccgaca gtcccactgc gatagtatcc tcatcctgaa tcatatcgta
    23701 atcatgtatc gcttttctca tctggcttat aactaaaccg ctcattaccg aatgatacca
    23761 ttttagtact tgaatttcaa ttatatgtaa tccttgccgg tcagatatct gtatagcatc
    23821 atatatccca tgcccgttct gggtatatcg gactttatct caaaatagct gccgtctttt
    23881 aaggaaaaca gtattctcat catttttgta acggagaatc ccgctatatc ggaaagaggg
    23941 aaaacatccc gtctggaatt atcttcggaa gttatctcca atctgtcgga atacagttcc
    24001 atagtgccgg tatttatcaa atccacacct ttttccacct ttattctgtt gagagtctga
    24061 ccgggatgtc tgaatatggg tttgtcaata tactcggttt taagtttatc aaggttattc
    24121 ttaagatact ctcgttgcca catatcccag tcatatacat tttcaaaatc aaactcatca
    24181 tccgactgta cttcaaagca gcagtattca tttaacttta cgcttaatcc gcatgtgcag
    24241 ctgaatatat cgcctgcact ttttaaggtg tctatggctt tacacttggg gcatacgaat
    24301 aataccgtct caagatattc ggccagattc ttccctttgt atctttgttt atactttctc
    24361 tgccagtcaa atgcattggt ataaagatcg gtgcggataa tatcggttat ttcttcttcg
    24421 gtcatggatt gcagttgctc ccttgagtac tcattgacta catggccgaa ttccggtcct
    24481 tttcttgcat gtttagccca tctgggtgta cggaagtatc cgccgtccag ccggtaagtt
    24541 ataagggcgg catcggagtg ctttaccagt gctccgtttg tcggtgaaat ccatccggtc
    24601 tgtccgttaa aggatctgtt tccttccaca aacatacaga cattggcacc ctgctccagt
    24661 ctcttgtgaa tatcttttac ggtacttttt gcttcggcgc cttttttgcg taccacaggg
    24721 taggccaaca atctgacaag ctttcctccc agtccgcttc tcaatatatg ttcgctcgct
    24781 acataaaaca tgtgcccctt aatagcaacg cctaccgcaa attggtcacc gtcggaattg
    24841 tgatttgaaa ggagaataaa aggtttgttt ttcggtttgt actttttgta gtgatagttg
    24901 tgaaaatact tgtaatatac tgtaaagggg attctcaaaa cggcatatac cgccttaaac
    24961 caggttagat atttcatgac attctccgta atcttagtat ttttatggta tcagaataaa
    25021 tgtgtaaaag caaataaaga atatagagat cggtttaata tcaatgccta taaaatgtat
    25081 tatagttgat tccgtataac aataaatata caatgagttt cgaggaaaca actgctatga
    25141 atgaaaaaac tgatttcgct gttgaaactg ataaaaacaa aaaagagagt aatcttttgc
    25201 aaatcaaggc ggatgatccg aataaggaaa cacttctggc tttattagag tctgaaagta
    25261 tttctcaaaa cccggatacg ataggctata cgaatgttga agatgcctta agaaacttaa
    25321 aaaacaatct ttaaaaaacg acttgtacaa ctgttttttt aaagactgta aatatattat
    25381 gttaggatat tgtttttttt cattagacaa ataaaaactt gatattaaga gaatattatg
    25441 ttatattata taccaaaagg aggtgttagt gtgctttcat atataagatt aagaaacttc
    25501 aagtcgtttt ctgacattat gttcgatttg cgaggaaaag ggggtatccc caaaaagaaa
    25561 gtattcgttt acggagaaaa cggttccgga aaatccaacc ttatctcttc actgtttttt
    25621 ttaaggaaaa ccgtagaatc aatgaggaga caaacagatt taaaaaaaat taccgatgta
    25681 tatctttccg aggaatatga taaagcaaca atattaaatg aaagaagagg aattttagaa
    25741 gatttaatta aaaatacact atatacttcg gagaatttga taaaagaata taaaacaata
    25801 aacagcaaag gcgggattga aattgaagta ggtttttttc ttggcggtgt tgacggtaca
    25861 tattctttat cctttgatga tgcaagggtt gtgcgggagg aattgaggta taagttgaat
    25921 gaacgtacag gtaccgtttt ccaattatcc gaaggcaata taagaatgag caaagcaata
    25981 ttttttgatg acacatacag caaagaatta aaacaaaata ttgaaaaata ttggggtatg
    26041 catactttta tgagcatatt agataatgaa acggctacaa aaaacaataa atttattgag
    26101 ttaagagttc ataaaaacat gctggatatt cttaaatggt tccaaggata ttcggtatca
    26161 tgtagggaag ctcatttgga aaagataaag cccggcactc ctttttctct ttttagggat
    26221 ttagagagcg gatatgtcag aatcggggat gacaaggaac tttttacttt agaaaagtta
    26281 ttgaacaatt actttacttc tctgtattcc gatgtgaaaa aggtctatta caaatttgaa
    26341 acaaaagaag gcagattgca atataagtta tatcttaaaa aattgttaaa cggaagtatc
    26401 ttggatattc cttttgaatt ggagtccaca ggaacacgga agttgctgag tatattgcct
    26461 tatattttgt cagccgtttc aggcaaaaca gtttttattg acgaaattga tggcggaatt
    26521 catgatttgt tgatgtgcga agtaattgag tcattaggtg atgctcttga tgcttcacaa
    26581 accggacaat taattgctac aacacacaat acccggatta tgaaacaatt ggcaaacgat
    26641 gatgtatata ttctgagtat agatgttgac ggtaataaaa atatattctc tgtggataaa
    26701 tatgatttca gaacgcaaaa aacacacagt atacaatcta agtatcttaa cggagattat
    26761 gcaggagttc cctttaccgg atatttggat ttttccgaga tggtggagga gatagaggca
    26821 tgcatataat atattttgtt tacactttat catatattgt ggtaatatag gatacgaaag
    26881 gcggatctct gacttgctaa gagaattgag agagctgttg aatgagcaat taggatgcct
    26941 ttttggagtt atcagtatga aggggacaat tttattgtgt tctgatagtt cccaagaagg
    27001 aaaggatttt gaccaattcg acaagcttgt aaaagacact aatgataaac cggctgtaga
    27061 tgggctaatc ttcacaaaga taaaagctat cagaaatgta cagtattatg tgtttgttga
    27121 acagatggga gacaacaccg attttgtgtt gaatctttta gccctctatg taaaagatta
    27181 tcttaccaat aatatagaga agaccgataa ggaattcttt ttcaaacaga tactgcaaaa
    27241 cgatatatcc ggcaatgaat tgaataaacg caataaagtg ttccgtttgg ataccggagc
    27301 aaagaagacg gtagtgaccg taagaacggg aaatgtcccg gaataccctg tgagcgaaat
    27361 attgaagaat cttttccgcg acaccaaaaa caccgttatc accactttgg ataatgacga
    27421 caccgttgtt attcttgcgg caaaggaaga catcgagcag gtgttgctat ctgcggtatc
    27481 cacattggaa gagcagctta tggttccggt aaaggcatcc ataggaaagt atttcacact
    27541 gttgcaggat gccagaaact cctatataac atcggtaaaa gcttttgata taggttctct
    27601 gtacagtgaa ggttattcgg tctataatta taacaacata ggcattgcca gattctttca
    27661 tgatataccg atagagcagt tggagcactt catagaagaa acaattacct ccgaagtata
    27721 tgacaggata gatgatgaga caataaaaac catgcaggca tttttcgaga ataacttgaa
    27781 tataagcgag accgcccgga aaatgtatct gcatagaaat actttggttt acagaataga
    27841 aaagttcaac aagttgaccg gtctggatgt tcagaaattc gaatccgcag tgctgttcta
    27901 tacaatatgc cagataaaat gttttttgcg aaacatggga aaggattaat atgatagatt
    27961 tcgataatgt atcaatgata tataaaagcg gtaccgaagc gttacacgga gtaacctttc
    28021 atattgatcc gggcgagttt gtatttatta ccggtaagtc cggttcggga aagtccacaa
    28081 taataaaact actgatgaga gaggaaaaac ccacatccgg cgatatattc gtaaacggat
    28141 atgacttatt gagtttatca aggtatgatc ttcctcatta ccgcagaaga ctgggagtag
    28201 tttttcagga ctgtaggctg ttaatgaaca aaaccgttta tcagaatgtg gagtttgcta
    28261 tggaaattgt aggtgagtca aacagaacca ttaaacaggt ggttccgaga cttctgcatc
    28321 tggtggatct ggaaggtaag gaaaaatctt ttcccgatga gcttgcgggc ggggaacagg
    28381 caagagtagc tctggcaaga gcaattgcaa atgctcccga tattctgatt gccgatgaac
    28441 ctacgggaaa tcttgatccc aaaacaagcg tggaaatagt ggatcttttg tataaagtaa
    28501 accgattggg tactacggta gtggtggtta ctcatgatac tcgtatagtg gacaggttac
    28561 aacaaagagt aatccgcctg caggacggag aaatagtcag cgatacggaa ggaagtattt
    28621 acgaaaatga agcctgtgag tattaaatac attttaatag agtcctttaa caacattgtc
    28681 cggaacaagg tgttgtttat aacctctatc tttacggtgt gtgtgacgat actgctgatg
    28741 ggtgttgtgg gaatacttct tatgaatgta tcatataact ccgataccat gatggaggaa
    28801 atgcttaata taacggtgta tgtcataccg gaagcagatg aaaaaactct taacgatatt
    28861 caggatgttt tagtagccga tgcacatgtg acggaaatta aatatgtctc acagatggat
    28921 gcctatcagg cgggaataga acttgtaggt gaggaagtta tggcggaact ggggcatgaa
    28981 tttcttcccg catccttcat tgtaaaactt gatgacgtaa catatgcgca tatattcgtg
    29041 cgcaatatga aagcaatgtc caacatctat gaagtcaaat acagttccga agcatatgaa
    29101 agagccggcg ccatatccta cacgataaga tatgtctgcg gggtgcttac tgcactgttg
    29161 ggaattctat cgctgttcct tatatccaac accataaaga taactgtatc ggccagcaaa
    29221 gaggaagtta ccattatgag atatataggg gcctcggaat cgagaataaa agctccgttc
    29281 tatctgcagg gagcatttat aggattctta ggcgcaatta tttcttttat tatagccggt
    29341 ttcggatata attatatgta tacaaggtta gagggggact tgagcgaata tatgggtgta
    29401 ctggaactgg ttcccgttcc tcagatgctt cttgtattat tgggagtgtt tttactgtac
    29461 ggcatcggcc ttggtatact gggaagcttt ttcgcgatac gcaagtatct gaaagcttaa
    29521 aagaggagta tgcaaatgat gaagggtact gaaaagttaa gaaaatgtgc gattatactg
    29581 gtctttgtgc tgttgctttc ttttactcac gcaacatttt gtgtagggga cattaatgaa
    29641 gccaaagata atctcggaaa catacaggac cagcttaaaa agattgaaaa tgagattaaa
    29701 caaaagcaga gcataaaatc atattacgag agccttgcca agaatatgaa aaacagcatc
    29761 agttctcaag agatacagct tgctacatat aacgagtaca tagtggaggt taccggtaag
    29821 atagacagtc tgaccgaaac aatcgagcag gcggagcaag attatgccga aagaataaag
    29881 gtgttgaaag acagagttgt caatgcctat ataaacagca atctttccat gctggatgtg
    29941 cttatgcagt cctcatcttt aaaagaatat tacgactata ttgaacttaa taagcttatc
    30001 accaaacacg ataatctgct tattgaagaa atgaaagtaa tgatgaagga cataacggca
    30061 aaaagagaaa ccatggagat gctcaaagct tcatatataa ctctaagcga gcaggcacag
    30121 atagtgctca gcaacatgca gtccatgcag agtgatgctt tagaaacaat caatgtaacc
    30181 ggcagtacca taaaggttct caaagcaaaa gaggacgcat tggaagccga agaagcggaa
    30241 atgcttaatc tcatattgaa actgcaaagg gagattgact atatcggtgg acagatgaca
    30301 tggcctctgc cttcctggaa gcacattccc acgggtaacg gattgttcgg ctggagactg
    30361 catcccatat tcttcgaatg gagaatgcat aacggagtgg acctcggcgg agttatgggg
    30421 aaaaatatag tggcggccaa tgacggagtc gtatccatag catcatgggg agacgggtac
    30481 ggttattata cggcgataga ccacggcgga ggcgtaacca cactgtatgc tcactgttcc
    30541 aaactcttgg tcagtgcagg acaatcggtt aagaaaggac aggtaatagc tttgatagga
    30601 gctacaggat gggcaacggg accgcacttg cattttgaag tgataatagg cggaaaaaga
    30661 gtggacccgc tcctgtatat agatccttca agatagatta tagaaaaggt gaacaaattg
    30721 aaaaaaatac ttatcaaaaa aacaataacc atattaataa tgttagcggt aacatcattt
    30781 tctctgctga cagggtgctc acttatcaag gagcttacgc ctattgacag agcaatagcc
    30841 cgcaggataa caacggtgac tttgcccgag gcatctcctc agaatgttaa acttgaggga
    30901 gagtttaccg aagaacagct ggaaaaattc aatgaagtat ataaccttct tttgaaaatg
    30961 tccatttatg atatagacca tgaaaagatg atggaagcca ttatagcggg catgtcatcg
    31021 gtatttgatg catataccat gtatgttccg gaagagcatg cacagtccat aagagaatct
    31081 tccagcgggg aatataaagg gataggcgta accatcgtta ctccggagga cggtatagga
    31141 gcggaagtcg tttcggtcaa tcccacgggt gatgcatatg tcaagggagt caagcccggt
    31201 gacataataa taaaagtaga ggatatggaa ctcacaaaga gcatgtcttt ggaatacatt
    31261 gcttccatgg taaaaggtga agaaggtaca tatgtgaata taacaatatg gagaccttcg
    31321 gaggaaaagg aaatacaatt caacattatg aggatgctca taaatacgat agaagtaacc
    31381 ggcaagatga ttgaaggcac cgatatcggc catgtatata taagcagttt cagtgccgat
    31441 atgctggatg aatttgtgga tatatttaat gacctggttc ttaaacaggg aatgaaaaag
    31501 ctggtattgg atttaagaga taaccccggc ggagatttct atgctgcggt cagccttgcc
    31561 gatgctttca tagaagaggg tgtaataaca acacttgcag acggtaccgg atatgaagag
    31621 gaatacacat cggacagcta tgccgtaagc attcctacgg ttattcttgt caacgaaaat
    31681 tccgcatcgg catcggagtt gttcagcgga gccatgaaac attacggtct tgcaaccata
    31741 gtgggtaccc gaacattcgg caaaggtgta gcacagagta tatatgactt ggatgacgga
    31801 tcgcaattgc gtgttaccag ctaccaattc ctgctgcctg acgacacctg tattgacggt
    31861 ataggcatta cacccgatgt ggaagtatta ccgtttgagg gctatgaaga tgcctatgta
    31921 gactctttgg aattggagca tgatgcacag cttcaaaaag ccatagagat actggagagt
    31981 aaatgatgta tgattgcata gctgatgtct atgacattct catgaaagat gtgaactatg
    32041 cggcatatgc ggattatata gatgagataa tagcaagata tcagacaaaa aagagcgaaa
    32101 taatattgga tgtaggctgc ggtacgggca gccttacatg tgctttgcga gataaaggat
    32161 attcgatgat aggtaccgat ccttcgcaga atatgttgaa tattgcatta tcaaaagaca
    32221 cttcaaacat acagtacatc aaccaatcta tggagaattt ggatttgttc ggtaccgttc
    32281 agacagctgt cagtttattg gactgcataa atcatataac cgacagaaaa cggctgcaaa
    32341 aagcatttga cagagtgtca ctttttctgg aaagcgaagg cttgttcata ttcgatttga
    32401 atacggtata taagtttgag accgttcttg caaaaaacac atatttcgat ctttcggctg
    32461 atgtctccta tacatggatg aataaatata acaaaaagac cggattatgc agaatggaga
    32521 ttacgtattt tataaagaat caacaagggt gctataaacg atacgataca tgcaatattg
    32581 aaagggcata ttccgacaaa gagattattg atatggcaaa cagaagcggt tttgaattgg
    32641 cggatatgct ggacgattta tcttttgata aacccaagaa gaacagcaca aggaaattta
    32701 taatattaaa aaagaaatag gtgttttatg gacagaatag taacggcttt ggcatatgat
    32761 aaaaaggtaa gggtatttat tgcacttagt acaaataccg tcaatgaggc tatacgttta
    32821 cacaagctca atctgatgtc ggcaatggcg ttgggaagat tgctcaccgc aaccgcgata
    32881 atgtcttaca tgacgcaaag cgagagcatt gcggtacagg tgtcatcgga aggtcctatg
    32941 aaaggggtat tggcaatatc cgatgaaaaa ggcaaggtca aaggttatgt cacaaacaat
    33001 gactatacaa catacggtga aaacattact tcgctgggac aacttctcat gcccggaaaa
    33061 ctgatgatat ccagagattt cggtttaaaa gaaccttata ccggcataac ccgtctcata
    33121 accggtgaga ttgccgagga tatagcccga tactacatgg acagtgaaca gacacccaca
    33181 gcctgtgcat tgggaacata tttcgatgac aaaggaaaat tgttatctgc cggaggatac
    33241 ataatacagg tcttacccgg taccgaagac aggattctga acgagattga acaaagggta
    33301 tcactgacag gcaacatatc caaactcata tatgatcttg aaacgccgga gaatctcctt
    33361 aatttccttt tctccgaccg caaccccgag ataaccggca cacacggcat tgcattcaaa
    33421 tgcagctgca gtaaagagaa gatattggac aaactgctga ccatagacaa agaacaactg
    33481 aacgaagtgg ctaagcagca gtcaacaata gaagcggttt gtccgtattg caactctcat
    33541 tatcattatc acagtaagga tatactgaag ataatcgaat aagcagagga caatgtgaag
    33601 aacaaagaaa aagtaaagag aataacggga gttatagcag cggtaatact gttttgtgtc
    33661 gctatagcca tacttatttt ccttttccgt aaaggggata ttataaatac acccgaaaat
    33721 ccgacagggc aggtaacgga atcctctgac ttatcttccc ccacatctac acggcagtat
    33781 actccgattg aagggttgaa ccgatatctt ccccgtaaaa acaatcccca aaacgtaaca
    33841 cttacagctg ataaggataa ggtaataaat gatatgttta acagtaccgt tgataatgat
    33901 gccacagttt atatatcttt tcccgacagc tccattgcgt gttatgagaa tgataaattg
    33961 aatctgaata ccggaatatt tcatgaaaaa cataactata tagccacggt tacgattatt
    34021 tcggacagca taataaagtc gggttatctt ttggccgaca gagacggtac aatctatatg
    34081 aatgaaataa aaaagagt
//
LOCUS       DAOSOM010000021        33114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_1368_length_33114_cov_6.568922, whole genome shotgun sequence.
ACCESSION   DAOSOM010000021 DAOSOM010000000
VERSION     DAOSOM010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 33114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33114
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1368_length_33114_cov_6.568922"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..1103)
                     /locus_tag="PLF14_06175"
     CDS             complement(54..1103)
                     /locus_tag="PLF14_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT70912.1"
                     /translation="MGYKMGIIGYGGMASWHHKNSVKVPGLQVTSAFDIDSERLKVAE
                     ENGLKIYDNVDDFLKYGDFSIVLVATPNNFHKYYSIMAMDAKKHVVCEKPVAMSLTEL
                     DAMIAASQKNNVLFTVHQNRRWDRDYKIVKKVLTDNTIGKPYTIESRVHGQNGVMHGW
                     RAYKVAGGGMLLDWGVHLIDQILYMIDEKVTEVYCQLFSIKTPEVDDYFKLLLRFESG
                     LSAQIEVGTYCLEKMPRWYINADHGSLIVKNWECEGKITKASEIVMEWEPEVVQTVGG
                     PTRTMAPRPKETLEELALPEVQSQWIEYYMNVISYLNGKEDIIVKHEELRRVMKIIEA
                     AFESDKIHKSIKCFI"
     gene            complement(1233..1487)
                     /locus_tag="PLF14_06180"
     CDS             complement(1233..1487)
                     /locus_tag="PLF14_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70913.1"
                     /translation="MGICEKVFGFELTFKTSDKLFSYKSADKGTLLILDKCDIDAYEN
                     VLCNDFTLTLSNPPYHADFSQKRLFCIHGSKIDRFKVKER"
     gene            complement(1503..1913)
                     /locus_tag="PLF14_06185"
     CDS             complement(1503..1913)
                     /locus_tag="PLF14_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70914.1"
                     /translation="MKLKRVLSLVMVLVLLAGLCLSLAGCGEPETATYTITNTTPNTV
                     ALKWIRKVKIQQQWSDIKPGDVLEITMDIHKKDPNILSVFFGGFKVGRDVITPENGFV
                     DGGKYYIIIFRTPGASKDEYKIVPDDGSYVKPAQ"
     gene            complement(2167..3426)
                     /locus_tag="PLF14_06190"
     CDS             complement(2167..3426)
                     /locus_tag="PLF14_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT70915.1"
                     /translation="MKNKMNKTEWSWVLYDVANSAFTMLTTALVPIYFAAMVANTSTI
                     ESNEATALWGSANSVAILILALLSPVLGAIADYKGMKKKMFAIALSVGLLGAFAMAFM
                     NTWLSFLIIYIIARIGYSATNVFYDSMLTDVTTDDRMDRVSSNGYAYGYIGSCIPFIL
                     CLVVYIFQPFGLDDTSSMQVAFIITALWWLLFTIPLFKNVHQVHYIEKQKGIVKGAFL
                     RIANTFKNIKKKKDIFLFILAYFFYIDGVYTIITMATIYGTELGLDSVSMVLALLLTQ
                     FIAFPCAIIAGILGTKYGTLKIIKIFIGIYMFVAIFGFFLVSEWQFWVLAAVVGMAQG
                     GIQSLSRGFFGRIIPKEESNEYFGFFDIFGKLADIMGPLLMGLFGIITGHSRYGILSL
                     ILLFIIGFVLLSRLPEGFGEIKLAQGE"
     gene            complement(3468..3935)
                     /locus_tag="PLF14_06195"
     CDS             complement(3468..3935)
                     /locus_tag="PLF14_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70916.1"
                     /translation="MNNTDKAKTSTVIYVISLVTGSVIYWLILFSYITSAFAFALSAA
                     GYAISILFLLTRYNEYIINKDYRVMNKYCDDVMSVCPKCGHMHITSYSRAFNSRIFMN
                     IRKYLLFQKNVRSVHVIRIAKCNQCGHIWYGGYTSIDKINSSFDENCVQNNVS"
     gene            complement(4128..5378)
                     /locus_tag="PLF14_06200"
     CDS             complement(4128..5378)
                     /locus_tag="PLF14_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013411547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOT70917.1"
                     /translation="MKTKGLRLYGVNDLRLDEFELPEIKDDEILARVITDSICMSSYK
                     AAIQGAGHKRVPKDIAKNPVIIGHEMCGEILEVGKKYKGRYKKGQLFTMQTSLNHPSN
                     IYAAPGYSFRYTGGTIQNMIVAKEVMEMDCLLEYNGDSYYQGSLAEPYSCIAGAFAAM
                     YHTNRINYEHTMGIKEGGKMAILAGAGPMGLGAIDYAVNQKKKPSLLVVTDIDDARLV
                     RAAEIFSPEWAAERGVKLIYLNTGKAEDPVKALMELSDNKGYDDVFVFAPVKPVVEQG
                     DKILGYDGCLNFFAGPSDTTFSASFNFYNVHYNSTHVVGTSGGNTEDMKECITMFEEG
                     KLNPAAMVTHIGGLDSAAETTLNLPKIPGGKKLIYTGISMPLTAISEFKEKGYPELAK
                     ICDANKGLWCKQAEDWLLKHSKKI"
     gene            complement(5380..6402)
                     /gene="mreB"
                     /locus_tag="PLF14_06205"
     CDS             complement(5380..6402)
                     /gene="mreB"
                     /locus_tag="PLF14_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HOT70918.1"
                     /translation="MGLDIGIDLGTSSVLIYVKGKGVVLREPSVVSRDEDGKVKAVGE
                     DACLMIGRTPANIEAIRPLRDGVISDYEATEEMLKYFIKKVCGKKLLLYKPRIMICVP
                     SGITEVERRAVMDAADKVGAKEVYIIEEPIAAAIGAGLDITKAYGSMVVDVGGGTTDI
                     AVISLCGIVKSKSIKIAGDTFDEAIIRFMRRKYSLQIGERMAEEMKIRIGCVYPRKEE
                     VSMQVRGRNLVLGLPRTIEITSSELMEAFDEVVQQIVDGVKNVLEKTPPELSADIGDR
                     GIVLTGGGSLIYGLDTLLSIKTGLEVTISDDPISCVVLGTGEALENLDKILKLRVIKQ
                     EERSHR"
     gene            complement(6473..7504)
                     /locus_tag="PLF14_06210"
     CDS             complement(6473..7504)
                     /locus_tag="PLF14_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT70919.1"
                     /translation="MKKALVILLSVIMVLSFTGCAKDREVLNVYNWGEYIDMEVLDMF
                     EEETGIKVNYNTFVTNEDMYVKLSKGRVSYDVIFPSDYMIQRMIDEDMLQKINMDNVP
                     NYFNIADVHKNLSFDPDNEYSVPYLWGTVGILYNKTMVSDTVDSWDILWNEKYSQKIF
                     MQDSQRDSMMVALIRLGYSQNTTDQNQVNQARDLLIAQKPLVLGYLVDEVKDKMVQGE
                     GALAVVWSGEAVAAMELNEDLAFVIPKEGSNRWVDSMVIPKNAKNVSAAEKFINFMLR
                     DDIMVMNANETGYSITSKTAIELLDEDWRNNLVAFPTEEMMSECEFFEYNAEATVMYN
                     KAWTEVTAN"
     gene            complement(7501..8346)
                     /locus_tag="PLF14_06215"
     CDS             complement(7501..8346)
                     /locus_tag="PLF14_06215"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT70920.1"
                     /translation="MKKLLKKLYIFLILLFLYAPIIVLIVYSFNASKSRAVWQGFSLT
                     WYQQLFTDSKILSALETTFLIALIASVVATVLGTFAAMGMMKMKKKTFETVRTITYIP
                     VLNPDIVTGVSLMMLFAFIGIELGFVSMLLAHITFCTPYVIISVLPKLYQLDMSVYEA
                     AQDLGAKPFYALRKTIIPQLMPGIVTGFLLSFTLSVDDFVISYFTTGGHVENLSMIVF
                     SMARKGVNPKINAVSALLFVMVMILLLIVNKRELSSKKHAAKNKDTLLNREKQIIKYR
                     RNEGK"
     gene            complement(8343..9170)
                     /locus_tag="PLF14_06220"
     CDS             complement(8343..9170)
                     /locus_tag="PLF14_06220"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT70921.1"
                     /translation="MSKRRFAFPYLIWMTIFIVVPLFLIFFYAFTDNASGKTLFTLEH
                     VKAIFQPLYLKVIGRSFYMSLIATLVCFVMGYPAAMILASKDFSQKNTMLILIVLPMW
                     MNFLLRTYSWLSLLENEGLINRFLVSIGLGQVQLMYNNFAVILGMVYNFLPFMILPIY
                     SVLVKIDKSVVEAAKDLGANGARVFLRIIFPLSMPGVVSGITMTFMPALSTFVISRLL
                     SGGKITLIGNIIEQQFTITGDWGLGSALSLLLMVLIFISMIFINKYGDESVGAGGLL"
     gene            complement(9167..10246)
                     /locus_tag="PLF14_06225"
     CDS             complement(9167..10246)
                     /locus_tag="PLF14_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT70922.1"
                     /translation="MGKKIIELKNIYKRFGDFTAIDGINLYINEGEFLTLLGPSGCGK
                     TTTLRIIGGFEQPDEGDVLFEGVRINDVPAHKRPLNTVFQKYALFPHLTVGENVAFGP
                     TIRKISPDIIQKDVKKYLEIVNLEGYEDRDVSTLSGGQQQRVAIARALINKPRVLLLD
                     EPLGALDLKLRKDMQDELKTMHKDVGITFVYVTHDQEEALTLSDTVVVMNNGRILQIG
                     TPVDIYNEPNNAFVAYFIGESNIIHNSIMVKDKLVNIDGVDFNCVDTGFGQNTPVDVV
                     VRPEDVVLVPADKADLKGIVTLVTFKGVHYEIWVEGPTFEWKVHSTLPQTVGDELGIT
                     VDPYNIHVMKHEPLEIPSEETAVEV"
     gene            complement(10266..10805)
                     /locus_tag="PLF14_06230"
     CDS             complement(10266..10805)
                     /locus_tag="PLF14_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOT70923.1"
                     /translation="MDIGGKIKQLRLLNSLTQQELADRSELSKGYISQLERNIITPTI
                     ATLQDVLTGLGTNLAQFFSGTYASQIVFKKKDMVTKKDETEGRQIMWVVPDCQKNAME
                     PIIMTLLPGGVSYQDDPHEGEEFGYVLEGKIRLEIDDKKYTVTRGQSFYFKADTKHLI
                     RNNSDKKAVVLWVSTPPSF"
     gene            complement(10992..11591)
                     /locus_tag="PLF14_06235"
     CDS             complement(10992..11591)
                     /locus_tag="PLF14_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018211855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70924.1"
                     /translation="MKKVEEYKEIFDKYGGMMRTCQLNEEKVLYRPLQKLVDKGLVEK
                     VRYGYYQWIDHDDLSEISTIIRLFPDAVLCMYSALRYYEYSDRIPGEWHLAVSKHSGK
                     SRFKIDYPFVKPYYVEPSILELGLTYGDINGHEVRIYDKDRLICDCIRYRNRMDKEIF
                     NKAIQSYVTDPRKYIPNLLEYAEILRVKKPVKELVGIWL"
     gene            complement(11773..13056)
                     /locus_tag="PLF14_06240"
     CDS             complement(11773..13056)
                     /locus_tag="PLF14_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HOT70925.1"
                     /translation="MKALIKRAGNKPISDSELSKYLDKTLSRFTAGKKVQKILIVPPD
                     FTRFYSMSGKITSMLYAKLKDSCTVDILPALGTHMPMTEKELNDMFEGIPHDRFFVHD
                     WKNDVVKIGEVPKEFVNEVSEGLVNEKMDVEVNKMFLDKTYDLIISVGQVVPHEVVGM
                     ANYTKNIVVGIGGNNMINKSHMLGAFYGMERMMGRDHSPVRKVFDYAQEKYLSSFPLF
                     YILTVTTQNGPSADIHALYAGNGRDAFEAGVKDSLEKNFTFLDEEPEKIVVYLDEREF
                     KTTWVGNKAIYRTRMAIAEGGELIILAPEVKRFGEDDENDRLIRKYGYIGRENILKKV
                     DENDDLKNNLSVAAHIIHGSSDGKFNISYAVRHMTKEEIEKANFKYLDYDETVKKYDP
                     AKLKDGFNYVDGEKIFYISNPALGLWSSRKKFEEQ"
     gene            complement(13058..13909)
                     /locus_tag="PLF14_06245"
     CDS             complement(13058..13909)
                     /locus_tag="PLF14_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008975409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT70926.1"
                     /translation="MKLPFKINLKNKVVVVTGAGGVLCSMFAEALAEAGAKVALLDLN
                     YDAAKKVSDEINGKGGITIPVKANVLEKQSLLEAKAAVNKKLGKCDILINGAGGNNPR
                     ATTTKEYFYPEDTDTEGLVTFFNLDPQGVEFVFNLNFIGTLLPTQVFAEDMVGREGCT
                     IINISSMNAFTPLTKIPAYSGAKAAVSNFTQWLAVHFSKVGIRVNAIAPGFFITKQNE
                     ALLLNKDGSFTERSRKILAATPMDRFGKPEELIGALLYLVDERSSGFVNGVVLPVDGG
                     FSAYSGV"
     gene            complement(13921..15369)
                     /gene="gnd"
                     /locus_tag="PLF14_06250"
     CDS             complement(13921..15369)
                     /gene="gnd"
                     /locus_tag="PLF14_06250"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018013701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HOT70927.1"
                     /translation="MKKADIGLIGLAVMGENLVLNMESKGFAVAVYNRTVEKVEKFVQ
                     TRAKGKNIIGCYSLPELVANLKKPRKVMLMVKAGAPVDNMIDQLIPLLDKNDIIIDGG
                     NSNFNDTIARTERVEKAGLMYIGTGVSGGEEGALTGPSLMPGGSESAWPHVKDIFQSI
                     AAKVEDGTPCCDWVGENGAGHFVKMVHNGIEYGDMELICEAYNIMKTLVGMDTDSMHN
                     VFAKWNEGELNSYLIEITADILAFKENKEPLVEKILDKAGQKGTGKWTAISALDEGVP
                     LTLITEAVFARCLSAMKDERVEASRILNGPTVSFNEKHDAFIEDLRKALYFSKIVSYA
                     QGYALMKAAAASYDWHLNYGGIALMWRGGCIIRSAFLGKIKQAYDNNPEISNLLLDPF
                     FKQKADECHQSLRKIVSVCALNGIPIPAFAAALTYYDGYRSERLPANLLQAQRDYFGA
                     HTYERIDSERGVFYHTNWTGRGGETSASTYNA"
     gene            complement(15458..16906)
                     /locus_tag="PLF14_06255"
     CDS             complement(15458..16906)
                     /locus_tag="PLF14_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HOT70928.1"
                     /translation="MSINLVPMPNEFHTDYKLFFRRNILESITVEYDSEYSAEEYSIV
                     IDDKGAAVKCSTKAGENCAHQTLRQLEFGYSGNIPYLTIHDKPAFSHRGFLIDCCRHF
                     FTVEELKKMIDAAALFKFNKFHWHLTEDQGWRMEIKKYPLLTEKGSQRPYSNFGRYYD
                     SKPYGGFYTQEEMKDIVEYCRKKSIDVIPEIEIPGHSSACLHAYPHLSCTGKEVPVRT
                     HAGIFKDILCAGKEEVYEFLFNVIDEMTAIFPGEYIHIGGDEAPKDRWKECPHCNRKM
                     RELGLENYEQLQGYMLNRIYDHLQKKGKQAITWNESLRGGNLEKGIIVQNWMEKNDEC
                     TIRASQGGKIIVSDFFHYYVNYPLGMTPLKKTYMYEPIPSELHENMRKNIIGVETPVW
                     TEFIDNFEDMSKMCFPRMAAVAETGWTNRDKKDYVDFKHRLTYVIRMVEEMGLKYPDV
                     SQWDPRFLQSAKQVIKFAYNMYNKETVKALRK"
     gene            complement(17010..18551)
                     /locus_tag="PLF14_06260"
     CDS             complement(17010..18551)
                     /locus_tag="PLF14_06260"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT70929.1"
                     /translation="MLQIKDLSIYMKNDGRPLVERLTINLNSNDKVAVIGEEGNGKST
                     LLRAIYDVESISDYAVCEGAVFKKRCIIGYLPQLLEDKYLNRKLGEYFTLFHVTDAKN
                     AGQYARLLTELGLEVGKEDYARLLSTFSGGEKIKIQLLSILLSSPDVLLLDEPTNDID
                     IDTLVWLETFINTCRIPVMYVSHDEMLIENTANVILHMEMHKRKSECSHTISRLSYSE
                     YIKVRKYNLEKQDQIATMQRAEYAKQMERWKKIHDKVEKDQENISRQDPASGRLLKKK
                     MKSVKSQEKRFMREKEDFTEFSDAEEAIFLRFYDGQYINTSKRVLDMELDYLKIGDKV
                     LSADVKLYVKGGEHIGIYGKNGAGKTTLLRQILNHIVQDNNIRLGYMPQDYTDSMDNS
                     LTCIEFLAPSGTKADITKARQYMGGMKFTADEMIGPISGLSGGQKAKLYLLKIVLDGC
                     NVLVMDEPTRNLSPLSNPVIREQLKGFKGTIIAVSHDRKFLNEVCDKRYELTDKGFFR
                     TDMTW"
     gene            complement(18785..19855)
                     /locus_tag="PLF14_06265"
     CDS             complement(18785..19855)
                     /locus_tag="PLF14_06265"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOT70930.1"
                     /translation="MYNLDEYLIPLWEGNIVYDESIMVVRNRNGSISLQPLAYKATKI
                     ISVKNAALTVTYVEGTDYLLQDGMLKIQDNGNIFVMDYDDYFPVNPKEGEAFSTYFGF
                     TVWHEGAYFHDRQIVVTYEHKESDIYFPGIVKGDLVVFDKLRKKENLNMLFLGDSITF
                     GWNSSALVDVAPYLPSWDKLTALGIQKRYGYEKVIEGDQDFSGIGNIRYINTAVSGTS
                     ITWGLQNVQELVCNYRPDMVFLAFGMNAGGLDAATFKERLHKIIYEIRKDNSDCSVVL
                     VATTYPNPNLTRKTFYIQEYVHVLHQVKEESGNIAVSDMSTLHKKLLDVKPYHHMTGN
                     NINHPNDFLMRQYAIVTLHALK"
     gene            complement(20187..20588)
                     /locus_tag="PLF14_06270"
     CDS             complement(20187..20588)
                     /locus_tag="PLF14_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOT70931.1"
                     /translation="MKKILIIILMLAIITALVGCAEDKGVDVKNTYEKITATEAKKMM
                     DEKSGYIILDVRTKDEYKGGHIPGAVLLPNESIGKETIDILPDKDQIILIYCRSGRRS
                     KEAAAKLVNLGYTNIYEFGGIIDWTYDIVTD"
     gene            complement(21122..22219)
                     /locus_tag="PLF14_06275"
     CDS             complement(21122..22219)
                     /locus_tag="PLF14_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015566642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HOT70932.1"
                     /translation="MQEIKCPNCGKMFQIDETGYAQILLQVRDMEFKKELAEMKEKEL
                     KNAQTEKELAVTQAVQAKDKELSEKITEISNLESKLKLQEQASVLQKNNLIEKHKDEL
                     KLREDEINMLKDFKAKQSTKMVGESLEQHCMNQFNQIRMAAFPNAYFEKDNDAKSGSK
                     GDFIFRESSDEVEFISIMFEMKNEMDTTETKQKNESFFKQLDKNRTEKNCEYAVLVSL
                     LEMDNEFYNNGIVDVSYRYPKMFVIRPQFFIPIISLLRNAALNSLEYQKKLAIVENQQ
                     LDLRNFEENLSQFKSSFSYSLENAKNRFVDAIDGIDKTISTLTKIRENLLKSESHLNA
                     ANNKVEDVDIKKLTHNAPSVREKLEQIKSAR"
     gene            complement(22246..22392)
                     /locus_tag="PLF14_06280"
     CDS             complement(22246..22392)
                     /locus_tag="PLF14_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70933.1"
                     /translation="MELDEGAEITDEINEEFRDSLCSATSRETTTWTYDGEKFEVSSE
                     HELS"
     gene            complement(22543..22737)
                     /locus_tag="PLF14_06285"
     CDS             complement(22543..22737)
                     /locus_tag="PLF14_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70934.1"
                     /translation="MDNKGEKLDIEKLREALKAEAQGAYFVGGYGGALVESFDIDDAS
                     PEELISIATRYGIDSEDYCE"
     gene            23041..24777
                     /locus_tag="PLF14_06290"
     CDS             23041..24777
                     /locus_tag="PLF14_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70935.1"
                     /translation="MEQLYIKQILYVLNWIQKRCLNKDNSNHADLPEFLQNIPKPEEY
                     KNYPLEDLASYRYYAGFQIEIMVSRDKKNWAMRVQEIDRGPKPDKPDEARKPVPGRSF
                     ITDIGLRRNESSVEIATRTLVDEPEGTKEECDAFRQAFIKELIADKDIKLVNDGWPVR
                     EEHVEVKNKDNYKDFIKWLKSDKREALAVVYCASPKVIKVKIKEESEDNTQVHTENKD
                     ETNVIDYLDEFKKLALSNRGYAFFYHILPESRRETFLKDIAEINFSNKKTPVLDNEDL
                     FIIGAKKYKTSTVYAYNMNQHCTDDSTKATVIKTIRKYYERKDVSFFGVRFVPELKEV
                     LDREKEETKKSTLDLLEKIDGLEQELHSQKSDYEKNIADLNSEIRRIINQKDKEIQDL
                     KDDLIQQEKDSKSFVESQNRILNEKDYLIERLRNRKKRPKKFEDIPKWVNNSFKDRLI
                     MSERAQKEVNEITKEYDLDNICDALEYLAFEYRDLLRGNISVDMVNNRASKIYDRSYE
                     VTKVDVPGINNKTEYSFKYYKRDGTPCEDEEIDRHLYLNNSNRIYFIYDKEDKRIVIA
                     SLPHHLRTKSDR"
     gene            complement(24778..25437)
                     /locus_tag="PLF14_06295"
     CDS             complement(24778..25437)
                     /locus_tag="PLF14_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOT70936.1"
                     /translation="MIKFDDVKKNTEVKTYIAKADESLIALGYTEHSFAHVLKVATTA
                     KYILTTLGYSQKEIELAQIACYLHDIGNLINREDHAQSGAMLAFRILKDMNATPDEIA
                     TVITAIGNHDESTAFPVNCVAAALIIADKTDVRRSRVRNTDTAHFDIHDRVNYSVKKS
                     TVRINEDKTEICLSLTIDPRYYSVLEYFEIFLDRMILCRKAAESLNLKFKMNINNQQI
                     L"
     gene            complement(25434..26282)
                     /locus_tag="PLF14_06300"
     CDS             complement(25434..26282)
                     /locus_tag="PLF14_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HOT70937.1"
                     /translation="MEILYGNDVVKALSEQIRNQLAALSGYIPHLAIVRVGEKPDDLA
                     YERGAVKRFEKLGLRCTLHAFPESIDNEDFAFAFKKINDDNDVDGILLLRPLPKHLDE
                     KAITEMINPVKDIDCISPINMAKVFSGDDTGFAPCTAEAVIEMIDYAGIDLTGKRATI
                     VGRSMVIGKPVAMMLMKKNATITVCHTRTADMPAACKNAEVLVAAAGKARMINRNHVS
                     EGAYVFDVGINVDDEGRLCGDVDIEDISDIAALCTPVPKGVGTVTTSVLAKHVVKAAK
                     MRREKK"
     gene            complement(26287..26913)
                     /locus_tag="PLF14_06305"
     CDS             complement(26287..26913)
                     /locus_tag="PLF14_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HOT70938.1"
                     /translation="MFKNKSIEEFIDALSSKQPVPSGGGASALAGTMGTALASMVGNL
                     TLGKKRYADVQEDIIILQQKVSVLTEELMELIDKDAEVFLPLSKAYSLPSTTDEEKEY
                     KDKVMEEALNTACSVPVSIMEKSYEMLLLLGEYAEKGSRIAISDVAVSAVMLRAAITG
                     ASVNVYINTKAMRDRNRAEQLNSHCDDIMEKGKKLADEIFERVEKNLR"
     gene            complement(27060..27272)
                     /locus_tag="PLF14_06310"
     CDS             complement(27060..27272)
                     /locus_tag="PLF14_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015552243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOT70939.1"
                     /translation="MNKGTVKWFNSEKGYGFISNDEGGDDVFVHFSAINIDGYKTLKE
                     GQKVTFDVEEDPKNSKKLRAANVCLV"
     gene            complement(27789..28907)
                     /locus_tag="PLF14_06315"
     CDS             complement(27789..28907)
                     /locus_tag="PLF14_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT70940.1"
                     /translation="MRWLKFIMLFFIFSIALLIVFAYRNFHYDYGNDRFMENKGTEIG
                     FREQIYKTKDGSEIAYLEGPDNGEPLLLIHGQMVSKEDYAKVLPELSKHFHIFAVDCY
                     GHGNSSKDPTKYNILSIRDDMILFMHDVIKEKTFLSGHSSGALISSAIAAKNKEQVKG
                     LLLEDGPFFSTEPGRAEKTFSYLDFKTIHDFLLQSDEKNYTKYFLEHTYLKEVFNKDG
                     KDNWSFLVKNPFTKRIKVNSEKMPIVWYYPPEIGLNGLVFLTRNMQDGTGMYDLRFGN
                     TFYDFSWFEGFDQRGALSEIECPTIILHVAPSKETAPGYYDENGILLSAMDEKDAKEV
                     QSLIEGSILMSGYESDHNIHADLPEQFIEAILEMKRKD"
     gene            complement(28904..29161)
                     /locus_tag="PLF14_06320"
     CDS             complement(28904..29161)
                     /locus_tag="PLF14_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOT70941.1"
                     /translation="MKINQKNRSKLIIKNAEEKKEQGLFAWTAKVGSKGQIVIPKEAR
                     DLFQINSGDTLLLLGDQSQGIAIVSGDYMKDMLKKIKGKLL"
     gene            complement(29597..31720)
                     /locus_tag="PLF14_06325"
     CDS             complement(29597..31720)
                     /locus_tag="PLF14_06325"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70942.1"
                     /translation="MERYIFKQAEPVWAAGLQNEMNIRMGFYAQAGKGKTSVNLACST
                     AYQIYVNDTFAAAGPARAARGYYRVDEIDITSYLNKDINDVAVIVWGYCINAYSFLDQ
                     PSFLTCEIVCDNDVVAATGVKGFFAYLLDDYLKKVQRYTYQRGFVESYVLTSDSRDWM
                     TGGNTHQVSLEMSGKKEYITRNVLYPEYEKLCPMDNAVVGKHKPCKPETYRLPWHLKI
                     DNPDYKQYKTEELDENINERIQQIEYDTANAVETDISNGICLSESEYAVYEFEREATG
                     FISFRIVCKETCEFLMIYDEMLINGVPDPIRIHGLWVNKYVLEPGEYNLVFFEPVSMK
                     YLNVVMFRGRARICSLHMIEYKHAPVDKAIDTTDPDLKKIFDAAVETYRQNALDIFMD
                     CPSRERAGWLCDSFFASRVEYFLTGKSLIEKNFLENFLLPESYPSLPEGMIPMCYPAD
                     HVNKNFIPNWAMWFVLQLYEYKERSNDIHMVAAFKPKIYALLEYFERFLNKDGLLQNL
                     EKWVFVEWSFANKLVQDVNFPTNMLYALMLERVSQLYDDKDKLVQAEGIRKKIRELSY
                     DGEFFRDRMVFKNDVLEVSHERTEVCQYYAFFTKTATKKKYSKLWKTLVKDFGPDRVE
                     KKLHPEIYPANAFVGTYLRMELLTEQGLYKQLLNESVGFFKYMAERTGTLWENKTDGA
                     SLNHGFAAHAGIWIYNAVEALKEHI"
     gene            31866..33044
                     /locus_tag="PLF14_06330"
     CDS             31866..33044
                     /locus_tag="PLF14_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70943.1"
                     /translation="MSKKIVILILFALIASLFTSCNCSYVPESDSHNTESSFSITSNS
                     DNEKPEEYYTAFRQGESSVSLYLVTPYGMYMNIRKYIYYSEKGNTEYIKLCNKPACNH
                     STEDCNAYTGAYQIGYYKDKIYYISRRDYYVNTLNCMNMDGTNHKEIKVLNNSCFSTF
                     GYFHNGYFYYIIGTPGVQAIGVTNDDNNLYRVKVDDNSKPEVVLTDDIIFNIQRFIVV
                     DDIIYLTVYKQDTTAECFLYSYSCKTGALTKIADNWSGNGFWGKHYGYCYLTNEGIYK
                     YNVETGEVTLEKAIKFDNHGYCGARFYPDYIYLIHNRNDDYRALREQDLVLYIYNWDY
                     EIIETVLLDFINKGKIGNFITDVGNYIIFASDMDNKPDYYIDKSEIGTDKFAFHKIEN
                     "
BASE COUNT         9631 a         7341 c         5454 g        10688 t
ORIGIN      
        1 atgaatcatc aatattcgtt atttctctgt tgacttttca tagttggtct cctttatata
       61 aaacatttaa tactcttatg tattttatct gattcaaatg ccgcttcaat tattttcatt
      121 actcttctta gttcttcatg cttaactata atatcttctt ttccgttaag ataggaaata
      181 acattcatat agtactcaat ccattggctc tgcacttccg gaagtgcaag ttcctccagt
      241 gtttctttag gacgcggtgc catggttctg gtaggacctc ctaccgtctg tactacttcc
      301 ggttcccatt ccattactat ctcgctggct tttgttatct ttccttcaca ttcccagttt
      361 ttcacaatca gcgaaccgtg atcggcgtta atataccatc tgggcatttt ttccagacaa
      421 taagtaccta cttcaatttg tgcggacaag cccgattcaa acctaagcaa cagtttaaaa
      481 tagtcatcca cttcaggagt tttaatactg aaaagctgac agtaaacttc cgtaaccttt
      541 tcatcaatca tatacaatat ctgatctatg agatgaacac cccagtccaa cagcatcccg
      601 ccaccggcca ccttgtatgc acgccatccg tgcataactc cgttttgtcc gtgtactctt
      661 gactcgatag tataaggctt gccgatagta ttgtcagtaa gaaccttttt cactatttta
      721 taatccctat cccatcttct gttctgatga acggtaaaca atacattatt cttttgggat
      781 gcagctatca tggcatccaa ttcggtcaga ctcatcgcta cgggtttttc acagaccaca
      841 tgctttttgg catccattgc cataatggaa tagtatttat ggaagttgtt gggtgtagcc
      901 acaagaacta tggaaaaatc accgtacttc aaaaaatcat caacattatc atatattttt
      961 aatccgtttt cctctgctac cttaagtctc tcggaatcta tatcaaaggc gcttgttact
     1021 tgcaaaccgg gcactttgac agaattttta tgatgccatg aagccattcc gccatatccg
     1081 attataccca ttttgtatcc catatgatac ctccatccat ttcatattat tacttataac
     1141 aagtcatgtc aacatcaaaa aaccgtaata cttgagtaat atttccgtaa aactctttta
     1201 ccggattttt atataatttt aagcatcagt ttctatcttt ctttaacttt aaatcggtcg
     1261 attttactcc catgaataca aaataaccgt ttttgcgaaa aatcggcatg atacggagga
     1321 ttactcagtg tcagagtaaa atcattacac aaaacattct catatgcatc tatatcacat
     1381 ttatccaata ttaaaagagt acctttatcg gcactcttat atgaaaacaa tttatccgat
     1441 gttttgaagg tcaactcaaa gccgaatacc ttctcacata ttcccaatta aatacctctg
     1501 ttctattgtg ccggcttcac gtatgatccg tcatccggaa caatcttata ctcatccttg
     1561 gaagcaccgg gtgtacggaa aatgatgata tagtatttcc cgccgtcgac aaatccgttt
     1621 tcaggagtta taacatctct gccgacttta aaaccgccaa agaacacact taaaatatta
     1681 ggatctttct tgtgtatatc catcgttatt tccagcacat caccgggttt gatatccgac
     1741 cattgttgct gaatttttac tttcctaatc catttgagtg caaccgtgtt cggagttgta
     1801 ttggtgatag tgtatgtggc tgtttccggt tctccgcaac ccgcaagtga aagacataat
     1861 cctgctaaca gtaccaatac cataaccaga gaaagaactc tttttaattt cattatatat
     1921 aacccccctt tttttatatg ctttttagac ttcaccattt gtattctatc atttattcac
     1981 gtctcttact atatgaaaaa tatgacattc gttgtgttta aataacattg cttctaacaa
     2041 caccaagttt gcttatgcag gtatatccat tatatatgca taaactccat taaatttgct
     2101 catttttgaa tatttatgca gtatttcggc atacactgta ttatctgcca taaaatgcca
     2161 catttttcac tctccctgtg ctaattttat ttcaccgaaa ccttcaggca accttgacaa
     2221 cagtacaaac cctattataa acagcaggat cagagataaa ataccgtatc tggaatgtcc
     2281 tgttattata ccgaacaatc ccataagaag cggtcccatt atgtccgcca atttgccgaa
     2341 tatgtcaaag aaaccgaagt actcgttgga ctcttctttg ggaataatcc tgccgaaaaa
     2401 gcctcttgac agtgactgta tgcccccttg tgccattccc actacggccg ccagcaccca
     2461 gaactgccac tcacttacaa ggaagaaacc gaagattgcc acaaacatgt atattcctat
     2521 gaatatcttt ataatcttca gtgtcccgta ttttgttccc agtataccgg ctataattgc
     2581 acacgggaaa gctatgaact gagtgagtaa taaagcaagc accatggaca cagaatccag
     2641 tcccagttcc gtaccgtata tggtagccat cgtaataata gtatatactc cgtcaatata
     2701 gaagaaatat gcaagtataa acaggaatat atcctttttc ttctttatat tcttgaatgt
     2761 gtttgcaatt cttaagaatg caccttttac tatacctttc tgtttttcga tataatgtac
     2821 ctgatgcaca ttcttgaata aaggtattgt aaacagcaac caccacaatg ccgttattat
     2881 gaatgctacc tgcatggaag aagtgtcatc caatccgaaa ggttgaaaga tatagaccac
     2941 gaggcataat atgaaaggaa tacaactgcc tatatacccg tatgcatatc cgtttgaaga
     3001 tactctgtcc attctgtcgt ccgttgtcac atcggtaagc atggaatcat agaatacatt
     3061 ggttgcggaa tatccgattc tcgcaataat gtatattatc aggaaggaca gccatgtatt
     3121 cataaatgcc atcgcgaaag ctcccaacag acctaccgac aatgctatag caaacatttt
     3181 cttcttcata cctttgtagt ccgctatcgc acctaaaaca ggtgaaagca atgcaagtat
     3241 taaaattgcc actgaattcg cacttcccca tagtgctgtt gcctcattgg attcaatggt
     3301 acttgtattg gctaccattg cggcaaaata aatgggcaca agagctgtag taagcattgt
     3361 aaacgcggaa ttggccacat cgtacagcac ccagctccat tcggtcttgt tcattttatt
     3421 cttcattatt aaattccccc gtttgtacct gttgatgcta ttttacacta actgacgtta
     3481 ttttgcacgc aattttcatc aaatgaagaa tttattttgt caatactcgt ataaccgcca
     3541 taccatatgt gtccgcattg attgcatttg gcaattctga tgacatgaac agaccggaca
     3601 tttttttgaa acaagagata ttttctgata ttcatgaata ttctgctgtt aaatgctcta
     3661 ctgtaagagg taatgtgcat gtgcccgcat ttaggacata ccgacataac atcatcacag
     3721 tatttattca taacccggta atctttattg ataatgtact cattatacct tgtcaacaaa
     3781 aacaaaatgc tgattgcata acccgccgcc gacagtgcaa atgcaaaagc tgaagttatg
     3841 taggagaaca gtataagcca gtatataacc gaacctgtca ctaatgagat aacatatata
     3901 accgtacttg ttttcgcttt atccgtattg ttcatatcat gtaaccgcct ttctcaggat
     3961 aaatacataa taacaagagt tgtgtactat aatacggact tatatcgtat taatcggact
     4021 ttgtatattc tatttaaatt tcatcaaaaa agagtaaata catttaaaga gcagataaac
     4081 tgctcttttc ttttaataaa taagattgaa tcaaatgaaa ctatcggcta tatcttctta
     4141 ctgtgcttca aaagccagtc ttccgcctgc ttacaccaca atcccttatt tgcatcacaa
     4201 atttttgcca gctcgggata tcctttttct ttaaattcgc tgatagcggt aagaggcata
     4261 cttattccgg tatagataag tttcttgcct cccggtattt tgggaagatt taatgttgtt
     4321 tccgcagcgg aatccaagcc gccgatatgt gtgaccatag ctgcaggatt gagttttccc
     4381 tcttcaaaca tggttatgca ttctttcata tcttcagtat ttccgccgct tgtccccact
     4441 acatgcgtag agttgtaatg cacattatag aagttgaaag atgccgaaaa agttgtatcc
     4501 gacggtcccg cgaagaaatt caggcatccg tcataaccta atatcttatc cccctgttcc
     4561 accaccggtt ttacgggagc aaacacaaat acatcgtcat atcctttatt atcggaaagc
     4621 tccataagtg ctttaacggg atcttctgct tttcctgtat tgagatatat aagtttgaca
     4681 ccgcgttcgg cggcccattc gggagaaaat atctcggccg ctcttaccag tctggcatca
     4741 tctatatcgg taacaaccag aagcgaaggt ttctttttct gattaactgc ataatctatt
     4801 gcacccaaac ccattggtcc cgcgcctgcc agtattgcca tttttccgcc ttcttttatg
     4861 cccattgtgt gctcataatt aattctgttc gtgtgataca ttgcggcaaa tgctccggct
     4921 atgcaggagt aaggttccgc caacgaacct tggtaatagg aatctccgtt atattccaag
     4981 aggcagtcca tttccataac ctctttggct actatcatat tctgtatcgt tccgccggtg
     5041 tatctgaatg aataacccgg agccgcatat atgttggaag gatggttaag gctggtctgc
     5101 attgtaaaaa gctgtccttt tttatacctg cctttatatt tcttgcctac ttccagtatc
     5161 tctccgcaca tctcatgccc tattattacg gggttctttg cgatatcttt cggtactctt
     5221 ttatgtccgg ctccctgtat ggcggctttg tatgatgaca tacatatact gtcggttata
     5281 actcttgcca atatctcatc atcttttatt tcaggcagtt caaattcgtc cagacggaga
     5341 tcgttcacac cgtataatct taatcctttt gtcttcatgc tatctgtggc tcctttcttc
     5401 ctgtttaata accctaagct tcaagatttt atcaagattc tccaatgctt ctccggttcc
     5461 taaaaccaca caggatatgg gatcgtcgga aatggtaacc tccagaccgg tcttaatgga
     5521 aagcaaggtg tccagcccgt atataaggct gccgccgccg gtcaatacta tacctctgtc
     5581 acctatatcc gcagataact ccggaggtgt cttttccagt acattcttaa caccgtcaac
     5641 aatttgctga actacctcat caaaagcttc cataagttcc gatgaagtta tttcgatggt
     5701 tctgggcaat cccaggacca gattcctgcc tctgacctgc attgagacct cttcttttct
     5761 gggatatacg caaccgatac gtattttcat ttcctcggcc attctctcac ctatctgaag
     5821 gctgtatttt cttctcataa acctgataat ggcttcatca aatgtatccc ccgctatttt
     5881 tatggatttg cttttaacaa ttccgcaaag agaaataacc gcaatatccg ttgttccgcc
     5941 gccgacatcc accaccatac ttccgtatgc cttggtaata tccagtccgg ctcctattgc
     6001 ggctgcaatg ggttcttcga taatatacac ttcttttgca ccgactttgt cggccgcatc
     6061 cataaccgct cttctttcaa cctcggttat cccgctgggt acacatatca ttatgcgcgg
     6121 tttgtagagc agaagtttct ttccgcaaac cttcttaata aagtacttga gcatttcttc
     6181 cgtggcttca taatcggata tgacaccgtc acgaagcggt cttattgcct caatattggc
     6241 aggggttctg cctatcataa gacatgcatc ctcacctaca gccttaactt ttccgtcttc
     6301 atctcttgat acaacggaag gctcacgtaa aaccacacct tttcctttaa catatataag
     6361 taccgaagaa gtaccgagat ctattccaat atccagtccc aaactattgc tccttacata
     6421 ttatttattc taattatata ctataaatca tataataaaa aactatattt atttaatttg
     6481 cggtaacttc cgtccatgct ttgttgtaca taaccgtagc ttcagcgtta tactcaaaaa
     6541 attcacattc ggacatcatc tcttccgtag ggaatgcaac aagattattc cgccagtcct
     6601 catccagcag ttcaattgcg gtttttgaag taatggaata accggtctca tttgcattca
     6661 tgaccattat atcatctctt aacataaagt taatgaactt ttccgctgcc gatacattct
     6721 ttgcattctt tggtattacc atactgtcca cccatctgtt acttccttcc ttgggaataa
     6781 caaatgcaag gtcttcattt aattccatag cggcaaccgc ttcaccggac cataccacag
     6841 ccaatgctcc ttcaccctgt accattttat cttttacttc atctaccaga tatcccaata
     6901 ccagcggctt ttgtgcaatt aaaagatccc ttgcctgatt tacctgattc tggtcggtag
     6961 tgttctgtga gtagcccaat ctgataaggg ccaccatcat ggagtccctt tgactgtcct
     7021 gcataaatat tttttgcgaa tatttttcat tccataatat atcccaactg tctacggtat
     7081 cgcttaccat agttttgttg tacaatattc cgacggttcc ccaaagatag ggaacggagt
     7141 attcgttatc ggggtcaaac gaaagattct tgtgcacatc ggctatattg aaatagttgg
     7201 gtacattgtc catattaatt ttttggagca tatcctcgtc aatcattctt tgtatcatat
     7261 agtcggacgg gaagataaca tcatatgaca ccctaccctt ggaaagcttc acatacatat
     7321 cttcattcgt tacgaatgta ttgtagttaa ccttaatacc ggtttcttcc tcaaacatgt
     7381 ctaaaacttc catgtcgatg tattcgcccc agttgtagac attcaatact tccctatcct
     7441 ttgcacatcc tgtaaaggat aataccatta tcacagataa cagaataacc aatgcttttt
     7501 tcattttcct tcatttctcc tatactttat aatttgtttt tctctattca acaacgtgtc
     7561 tttgttcttt gctgcatgct ttttggacga aagttcacgt ttgtttacaa taagcagcaa
     7621 tatcattacc attacaaaga gtaatgccga aactgcattt atcttgggat taaccccttt
     7681 tcttgccatg gagaatacta tcatggaaag gttctccaca tgtcccccgg ttgtgaaata
     7741 gcttataaca aagtcatcca cactcaatgt aaaggataac agaaatcccg taactattcc
     7801 cggcatgagc tgaggtatta tggtttttct cagtgcataa aacggtttgg cacctaaatc
     7861 ctgtgcagcc tcataaaccg acatatccaa ctgatacagt ttgggtaata ccgagattat
     7921 tacataaggt gtgcagaatg tgatatgtgc caacagcata gacacaaacc ccaactcaat
     7981 accgataaat gcaaacaaca tcatcagaga aacacccgtt acaatatcgg gatttaagac
     8041 gggaatatat gttattgttc ttactgtttc aaaggttttt ttcttcattt tcatcatgcc
     8101 catggcggca aaagtgccta aaacggtagc taccaccgaa gctataagag ctataagaaa
     8161 tgtggtttcc aaagcgctca gtatcttact gtcggtaaaa agttgctgat accatgttaa
     8221 agaaaaaccc tgccatacag cgcgcgactt ggacgcattg aatgagtata ctatcagaac
     8281 gataatggga gcataaagaa acagcagtat cagaaaaata tataatttct ttaacagttt
     8341 cttcataaca gacctcccgc tcccaccgat tcatccccgt atttattaat aaatatcatg
     8401 gatatgaata taagtaccat tagaagcagt gataatgccg aacccaatcc ccagtcaccg
     8461 gtaatggtaa actgctgctc gattatatta cctataagcg taatcttacc tccgctcagc
     8521 aatcgggata taacgaaagt ggacaaagcc ggcataaaag tcattgtgat accggaaaca
     8581 actccgggca tacttaacgg gaatattatg cgtaggaaaa cccttgcacc gttggctcct
     8641 aaatcttttg ccgcttctac tacactctta tcaatcttca ctaaaacgga atatatgggc
     8701 agtatcataa aaggcagaaa gttatacacc atgcccaata tcacggcaaa attgttatac
     8761 atgagctgaa cctgccccaa gcctatagat accaggaatc tgtttatgag accttcattt
     8821 tccaacagtg acagccagga gtatgttctc aacagaaaat tcatccacat gggtaatact
     8881 atcagtataa gcattgtgtt cttttgggaa aaatctttac ttgcaagtat catagctgca
     8941 ggatatccca ttacaaagca taccaaagta gctataagtg acatataaaa ggatcttcct
     9001 attaccttca gatacaaagg ctggaatatc gcctttacat gctccaatgt aaagagtgtc
     9061 tttccggagg cattatctgt aaaggcatag aaaaatatta aaaaaagcgg aacgacgata
     9121 aagattgtca tccagataag ataaggaaaa gcaaaccgcc ttttactcat acctccaccg
     9181 ccgtttcctc agacggaatc tccaacggtt catgtttcat aacatgaata ttgtaaggat
     9241 ccaccgtaat acccaattca tcaccaacag tttgaggtaa tgtggaatga actttccatt
     9301 cgaaggtcgg gccttccacc catatttcat agtgtacacc tttgaaagtg accaatgtaa
     9361 ctataccctt gagatccgct ttatctgcgg gaacaagtac cacatcttcc ggtcttacta
     9421 caacatcaac gggtgtattc tgtccgaatc ccgtatctac acagttaaaa tccacaccgt
     9481 ctatgtttac caacttatct ttaaccataa tgctgttgtg tattatatta ctctccccta
     9541 tgaaatacgc aacaaatgcg ttattgggct cgttatatat atcaaccgga gttcctatct
     9601 gcagtattct tccgttattc attaccacta cggtatccga caaggtcaat gcctcttcct
     9661 ggtcatgtgt tacatatacg aaggtaattc cgacatcttt atgcatggtc ttgagttcat
     9721 cctgcatatc tttgcgcaat ttcagatcca aggctcccaa aggctcatct aacagcaata
     9781 ccctgggttt gtttataagg gcgcgtgcta tggccactct ttgttgctga ccgccgctta
     9841 aggtggagac atctctgtct tcataacctt caagatttac tatttccaga tactttttca
     9901 catccttctg aatgatatcg ggactgattt ttctaattgt gggaccgaat gcgacattct
     9961 cgcccaccgt aagatgcgga aataatgcat atttctgaaa aacggtgtta agcggtcttt
    10021 tgtgtgccgg gacatcattt atccttacac cttcaaaaag cacatcgcct tcatcaggtt
    10081 gttcaaaacc cccgattatt ctcaatgtgg tggttttacc gcaccccgaa ggacccaata
    10141 atgttaaaaa ctcaccttcg tttatgtaca gatttatgcc gtcaattgcg gtaaaatctc
    10201 cgaatctttt gtaaatgttt ttaagttcga taatcttctt tcccatgtct tctccaaagt
    10261 tatatttaaa agctcggcgg tgtggacacc cacaaaacca ctgctttttt atcggaatta
    10321 ttacgtatta aatgtttagt atcggctttg aagtaaaaag actgtcccct tgtgaccgta
    10381 tatttcttgt catcaatctc cagtcttatt ttaccttcca gtacatatcc gaactcctca
    10441 ccttcatgcg ggtcgtcctg ataacttacc ccgcccggta acaatgtcat gatgataggc
    10501 tccatagcgt tcttctggca gtcgggtact acccacataa tctgccgccc ttcggtttca
    10561 tctttctttg taaccatatc ttttttctta aatacaatct ggcttgcgta tgtaccggaa
    10621 aagaactgtg caagattggt gcccaatcct gtgagcacat cctgtaatgt agcaatcgtc
    10681 ggtgtaataa tattcctctc cagttgcgat atatatccct ttgagagttc acttctgtct
    10741 gcaagctcct gttgcgtaag agagttcaat aaacgaagct gctttatctt accgcctata
    10801 tccatcacta cccttccttt caaccatact atacttaact ttatgttaag taatactaaa
    10861 ctcaactgtt attatatgac cttttcacca agtgtcaata agataatgaa aatattattt
    10921 ttaatcggta tgccgcatat tgattttgca catacattgc gcaaaggata ggaactttat
    10981 cagatatttc atcatagcca tatccctacc aattccttta caggcttttt tacacgcagt
    11041 atttcggcat attccaacaa atttggaatg tattttctcg gatctgtaac ataactctga
    11101 atcgctttat taaaaatctc tttgtccatc ctgtttcgat accgtatgca atcacaaatc
    11161 agtcggtctt tatcataaat acgcacttca tgtccgttaa tatctccgta tgtcaatcca
    11221 agttccaaaa ttgaaggctc cacatagtag ggttttacaa acgggtagtc aatcttaaag
    11281 cgtgacttac ccgagtgctt acttacggcc aagtgccatt cgccgggaat tctatcgctg
    11341 tattcgtaat atctgagagc ggaatacatg caaagtactg catcgggaaa gaggcgaatt
    11401 atggtagaaa tctcactgag gtcatcatgg tctatccact gataatatcc gtagcgtact
    11461 ttttctacaa gacctttatc cacaagtttt tgaagcggac gataaaggac tttctcttca
    11521 ttcagctggc atgtcctcat cataccgccg tatttatcaa atatctcttt gtattcttca
    11581 acttttttca tttccatcac ctgcactact tttacccaaa ctaagcaaac aagcggataa
    11641 agtaatgctg ttttattaat aatatcaaat aatgcggaaa aataaaagtc acccctcacg
    11701 gaatgacttt aaaactatat ctgcttaatc ttatatatta atttgtaaac ttacaaaaca
    11761 gcttagacta tatcactgtt cttcaaactt cttccttgaa gaccataaac ctaaagcggg
    11821 attggaaatg tagaatattt tttctccgtc cacataattg aatccgtcct taagtttcgc
    11881 agggtcatat ttctttaccg tttcatcata atcgagatac ttgaaattgg ccttttcaat
    11941 ttcttctttt gtcatatgcc ttaccgcata agatatattg aactttccgt cactggaccc
    12001 atgaattata tgtgcggcta ccgacagatt attcttcaaa tcgtcatttt catcgacttt
    12061 tttcagaatg ttctctcttc ctatatatcc gtacttcctt ataagtctgt cattttcatc
    12121 gtcttcaccg aaacgtttta cctcgggagc aagaatgata agttccccgc cttcggcaat
    12181 ggccattctg gtacggtata ttgccttgtt tcccacccag gtggtcttaa attctctttc
    12241 atcaagataa accactattt tctcaggttc ttcatccaga aatgtgaagt ttttctccag
    12301 actgtctttc acgcccgctt caaaagcatc ccgaccgttt cccgcataca gtgcatgaat
    12361 atcggctgac ggtccgttct gagttgttac ggtcagtata tagaaaagcg gaaaggaaga
    12421 taagtatttc tcctgagcat aatcaaatac ttttcttacc gggctgtggt ctcttcccat
    12481 cattctctcc attccgtaga atgctccgag catgtgagat ttgtttatca tattgttgcc
    12541 gcctattccc acaacaatat tcttggtata gtttgccatg cccaccactt catgaggtac
    12601 cacctgaccg acggaaataa tgaggtcgta tgtcttgtcc aaaaacattt tatttacttc
    12661 cacatccatc ttctcattta ccagcccttc ggatacttca tttacgaatt ccttcggaac
    12721 ttcccctatc ttcacgacat cgtttttcca gtcatgaacg aaaaacctgt catgaggaat
    12781 gccttcaaac atatcgttca gttccttttc tgtcatgggc atatgtgttc ccagggcagg
    12841 taatatgtct actgtacagc tgtctttaag cttggcataa agcatggaag taattttccc
    12901 ggacatggaa tagaacctgg taaaatccgg cgggacaatc agtattttct gcactttttt
    12961 acccgctgtg aatcttgaca aggttttatc taagtactta ctaagttcac tatccgaaat
    13021 gggtttatta cctgctcttt ttattaaagc tttcatgtta caccccgctg tatgccgaaa
    13081 atcctccgtc aaccggaaga accactccgt ttacaaagcc ggaagaccgc tcatccacaa
    13141 gataaagcaa tgcccctata agttcttcgg gttttccgaa tctgtccata ggagttgctg
    13201 caagtatctt tctggatctt tcggtgaacg aaccgtcttt attaagaagt aatgcttcat
    13261 tttgcttggt tataaagaag ccgggggcta tggcatttac tctgataccc actttggaaa
    13321 aatgcacggc cagccactgc gtgaaattgg atacggcggc tttggcaccg gaatatgccg
    13381 gtatcttagt caacggcgta aatgcattca tagaagatat gtttataatc gtacaacctt
    13441 ctctgcccac catatcctca gcaaatacct gtgtcggaag cagtgtaccg ataaaattga
    13501 gattaaacac aaactccact ccctgagggt caagattaaa gaatgtaacc aacccttcgg
    13561 tatccgtatc ttcgggataa aaatactctt ttgtggttgt tgctctggga ttgttgccgc
    13621 ccgcaccgtt tataagtatg tcgcattttc ccaacttttt attaactgcg gctttggctt
    13681 ccagaagact ctgtttttcc agtacattgg ctttaacagg tatggttatt ccgcctttac
    13741 cgttaatctc gtcggaaact tttttggctg catcataatt aaggtccaaa agtgcaactt
    13801 ttgcacctgc ctcggcaaga gcttctgcaa acatggaaca taatactcct ccggcacctg
    13861 taaccacaac aaccttattt tttaaattaa tcttgaatgg taatttcatt tcccgcctcc
    13921 ttacgcgtta tatgtagatg cggatgtctc tccgcccctt cccgtccagt ttgtgtgata
    13981 gaatactcct ctttcactgt caattctttc atatgtatgt gcaccgaaat agtctctttg
    14041 tgcctgtaat aaattcgccg gcaatctctc gcttctgtaa ccgtcgtaat atgtcagtgc
    14101 ggcggcaaat gccggtatgg gaatgccgtt aagagcacat accgatacta tcttccttaa
    14161 actttgatga cactcatcgg ccttttgttt gaagaaagga tccaacaaaa gattggatat
    14221 ttcgggattg ttatcatatg cctgctttat cttacccaag aatgccgaac ggataatgca
    14281 tccgcctctc cacataaggg ctatgcctcc gtaattaagg tgccagtcat aagatgctgc
    14341 agcggctttc atcaatgcgt aaccctgtgc gtaagacact atcttggaaa aataaagagc
    14401 ttttctcaaa tcttctataa aagcatcatg tttttcatta aacgacacgg taggaccgtt
    14461 aagaatgcgg cttgcttcaa ccctttcatc tttcatagcg gataagcatc ttgcaaacac
    14521 cgcttcggtt atgagggtaa ggggtacgcc ttcgtccaat gcggaaatcg ctgtccattt
    14581 acctgtacct ttctgtcccg ctttatctaa aatcttttcc accaacggct ctttgttttc
    14641 cttaaatgcc agtatatccg cagttatctc tataagatag gaattcagtt ccccctcatt
    14701 ccatttggca aaaacattat gcatactgtc ggtatccata cctacaagag tcttcattat
    14761 gttatatgcc tcgcaaataa gttccatatc gccatattca atgccgttat gaaccatctt
    14821 tacaaaatga ccggctccgt tttcacctac ccaatcgcaa cagggtgtgc cgtcttccac
    14881 tttggcggct atggactgga aaatatcctt tacatgaggc catgcgcttt cactgcctcc
    14941 cggcatcaaa gaaggacctg tcagggcacc ttcttccccg ccgctcactc ccgtaccgat
    15001 atacataaga ccggcttttt ccactctttc ggttcttgct atggtatcgt tgaaattgga
    15061 attaccgccg tcaattataa tgtcattctt atccagaaga ggtatcagct gatctatcat
    15121 gttatccacc ggagcaccgg cttttaccat cagcattact tttcttggct ttttcaaatt
    15181 ggcaacaagt tcaggaaggc tgtagcaacc aattatattc ttaccttttg ctcttgtctg
    15241 aacgaatttt tccacctttt ccaccgttct gttgtacaca gccacggcaa atcccttgct
    15301 ctccatgttg agtactaaat tttcgcccat tacggcaagg cctataaggc cgatatcagc
    15361 ttttttcata tctaaccctt tctgttttca ggtatataac ataccgtatt caactatgta
    15421 ttttataata caacagcctg tttgtccatt gatgcgctta ctttctaagt gctttcacag
    15481 tctctttgtt gtacatattg taggcaaatt tgattacctg cttggctgac tgcaaaaatc
    15541 tcggatccca ctgtgaaaca tcaggatatt ttaatcccat ctcctcaacc atacgtatta
    15601 cataagtaag tctgtgttta aaatcgacat aatccttttt atccctgttt gtccagcctg
    15661 tttccgctac tgcagccatt cgcgggaaac acattttact catatcctcg aaattatcaa
    15721 taaactccgt ccataccggt gtctccactc cgattatatt cttcctcata ttctcatgaa
    15781 gctcggaagg tataggttca tacatgtatg ttttcttaag aggtgtcatt cccaaaggat
    15841 aattcacata gtaatggaag aaatccgaaa caattatttt gccgccttgt gaagctctta
    15901 ttgtacactc atcattcttt tccatccaat tctgcacaat tatacctttt tccagattac
    15961 cgccgcgtaa gctctcgttc catgttatgg cttgtttacc tttcttttgc agatggtcat
    16021 atatcctgtt cagcatatat ccctgcagct gttcgtaatt ctccaacccg agctccctca
    16081 tctttctatt gcaatgcgga cattctttcc atctgtcctt gggggcttca tctccgccga
    16141 tatgtatata ttcacccggg aaaatagcag tcatttcatc tataacatta aataaaaact
    16201 catatacctc ttcttttccc gcacataaga tatccttgaa gatacccgca tgagtgcgta
    16261 ccggtacttc cttacctgta caggacaggt ggggatatgc gtgaaggcag gctgacgaat
    16321 gaccgggtat ctctatttcc ggtatcacat caatactctt ttttctgcaa tactccacaa
    16381 tatccttcat ctcttcctga gtatagaacc cgccatacgg cttagaatca taataccttc
    16441 cgaaatttga atacggtctt tgcgaacctt tttcggtaag aagaggatat ttctttatct
    16501 ccatacgcca tccctggtct tccgtaagat gccagtggaa tttattaaac ttaaaaagcg
    16561 cagcggcatc aatcatcttc ttcagctctt ccacagtaaa gaaatgcctg cagcaatcaa
    16621 tcagaaaacc tctgtgggaa aaagcgggct tatcgtgaat tgtaaggtaa ggtatattgc
    16681 cggaataacc gaattccagt tgccttaaag tctgatgtgc acagttctct cccgctttcg
    16741 tggagcactt aactgctgct cctttatcat caattacaat agagtattcc tcggctgaat
    16801 attccgaatc atattccacc gtaatgcttt ccaatatgtt tcttctaaag aaaagtttgt
    16861 aatcagtgtg aaactcatta ggcatgggta ccagattaat gctcatatag aacctccgaa
    16921 attaactcta tatatattct atcataaaag aataaaagat tattaatctg atagtataga
    16981 aacaactttt tcaaaaaaac atacacgctt taccatgtca tatctgtcct aaaaaatcct
    17041 ttatccgtca actcataccg cttatcgcac acttcgttta gaaactttct gtcatgggaa
    17101 acggcgatta tggtaccttt aaaacctttc agttgttcac gtatcaccgg attggataac
    17161 gggcttagat tccttgtagg ttcgtccatc acaagcacat tacatccgtc cagcactatt
    17221 tttaaaagat aaagttttgc tttctgtccg ccggacaaac cggaaatcgg accaatcatt
    17281 tcgtcagcgg tgaatttcat acctcccata tactgtcttg ctttggtaat atcggctttt
    17341 gttccgctcg gggcaagaaa ctcaatacat gtaagactat tatccatact gtcggtatag
    17401 tcctgcggca tatagcctaa acggatatta ttgtcctgta caatatgatt aagtatctgt
    17461 ctcaataaag tagtcttacc tgctccgttc tttccgtata tacctatatg ctccccgcct
    17521 ttaacataca atttcacatc ggctgaaagt actttatctc ctatcttcag ataatccaat
    17581 tccatatcca gtaccctttt tgatgtattt atatattgtc cgtcataaaa tcttaagaaa
    17641 atcgcttctt cggcatcgga aaactccgta aaatcttcct tttcacgcat gaatcttttt
    17701 tcctgtgatt taactgactt cattttcttc ttcagcaatc ttccgcttgc gggatcctgt
    17761 ctggatatgt tctcctgatc tttctccact ttatcatgaa tcttcttcca acgctccatc
    17821 tgttttgcat attccgctct ttgcatggtg gcaatttgat cctgtttttc cagattatat
    17881 ttcctcactt taatatattc ggaataactt aagcgtgata ttgtgtggct acattcactt
    17941 tttcttttgt gcatttccat atgtaagata acattagccg tgttttcaat aagcatctca
    18001 tcatgggaca catacattac cggaatccta caggtgttta taaaggtctc cagccataca
    18061 agagtatcta tatcaatatc attggtaggt tcatccagta gcagcacatc cggcgatgat
    18121 aacagtatag ataaaagctg aatctttatc ttttctcctc cggagaaggt agaaagcaac
    18181 cttgcataat cctctttccc cacttccagc cccaattccg tcaacaatct tgcatattgt
    18241 ccggcatttt ttgcatcggt tacatgaaat aaggtaaaat attctcccaa tttcctgttc
    18301 agatatttat cttcaagcaa ctgtggcaaa taccctataa tacaccgttt tttaaaaaca
    18361 gcaccttcac agactgcata gtcagaaata ctttcgacat cataaattgc tctaagcaaa
    18421 gtactcttgc cgttgccttc ctcgcctatt acagccactt tgtcattaga gttcaaattg
    18481 atggtaagcc tttctaccaa aggtctgccg tcattcttca tatatatgga taaatcttta
    18541 atctgcagca taaaatcccc caaaaaaatc cgtatgtcat ggaatgaaaa cggatgctgc
    18601 aataaaacat atttgacagt tataatcctt attctttcca agacataata aatacgggtc
    18661 tcccctgtta tctgttatgt tttgtctcga attaaagaat catttcctgc ctcttttctt
    18721 gaatattatc taaaaaatat cattaaaaca gcatattgtc aatcagtcaa ctcccaagtt
    18781 tttattattt caacgcatgc aatgtcacta ttgcatactg acgcatcaga aaatcattcg
    18841 gatgatttat attgttgccc gtcatgtggt gataaggttt gacatctaaa agtttcttgt
    18901 gcaaagtgga catgtccgat accgcaatat tacccgactc ctctttaacc tgatgcaaaa
    18961 catgtacata ctcctgtatg taaaatgttt ttcgtgtgag attgggattt gggtaagttg
    19021 ttgcgactaa aacaacggaa caatcggaat tatcttttct tatctcgtaa ataatcttat
    19081 gaagtctctc cttaaaagta gcagcatcca atccgccggc attcataccg aaggctaaaa
    19141 acaccatgtc aggtctgtaa ttgcacacca attcctgcac attctgcagt ccccatgtaa
    19201 tagatgttcc cgaaaccgca gtgtttatat acctgatatt tcctataccg gaaaaatcct
    19261 gatccccttc tataaccttc tcataaccgt atctcttttg tatacctaaa gcggtcagtt
    19321 tatcccacga gggaagatac ggagccacat ccaccaaagc ggaactgttc cagccgaaag
    19381 ttatgctgtc tccgaggaac aacatattca ggttttcctt tttcctcaat ttatcaaata
    19441 caactaaatc gcctttaaca atcccgggaa aatatatatc gctttcctta tgctcataag
    19501 ttactactat ttgcctgtca tgaaaatatg ctccttcatg ccagactgta aaaccgaaat
    19561 atgttgaaaa ggcctcacct tcctttggat ttacagggaa atagtcatca taatccataa
    19621 caaatatatt tccgttatcc tgtatcttga gcattccgtc ctgtaataga taatcggttc
    19681 cttccacata agtaacggta agagcggcat tttttactga aataatcttt gtcgctttat
    19741 atgccaaagg ttgtaaagaa atactgccgt ttcggtttct tacaaccatt atcgattcat
    19801 cataaactat attaccttcc cataacggta taagatattc atcaagatta tacatatttg
    19861 tcacctctgc ttaaatgaat tgctgatatt atatcatgaa gcaaaaaagc gtgtctttat
    19921 gcctttttga taggttatta cttccatatc cccgcaactc tagcgataac aaaaaagggc
    19981 cagaatagta tcataataac cagtttcaat gtgtaaagtt gcttaagtgt cattgttctt
    20041 ttcctccgca tatccatttt atcatcgcat gattatgtgc acaatataaa ataccgttac
    20101 aatatagtca ttgtacgtat gtgtccaaaa atattcccac taactatgtt tataggttta
    20161 aaaatactca atcaacctta caaaaatcag tctgtaacta tatcataagt ccagtctatt
    20221 atgccgccga actcatagat atttgtgtat cctaaattaa ccaatttggc agctgcttcc
    20281 ttgctgcgtc taccggacct gcaatagata agaataattt ggtctttgtc gggtaatata
    20341 tctattgtct ccttccctat actttcatta ggcagaagaa ccgcaccggg tatatgccct
    20401 cccttatact catctttggt cctcacatcc aagataatat atccgctttt ctcatccatc
    20461 atcttttttg cttccgtagc agtaatcttt tcataagtat tcttcacatc tactccttta
    20521 tcctcggcac aaccgaccag tgctgtaatt atagctaaca ttagtattat gattaaaatc
    20581 tttttcatgt cacacctcaa aattaatgaa ctacactaaa tacaatagca ataaaacacc
    20641 ggcttttcag tgacaaaagc acattatgca tataatatta tattcccata gaattacgtg
    20701 tttagaatta tgtttagatc aagcagaaag cttatatata caatcattaa tgttaaatct
    20761 gtaaaatgca gaatcaatat tattttccaa aacacctgtt aactgctcaa ttgttgaagc
    20821 atcagtttga gagcttttta atcatttaca tcagccatat tttaaactta agaacacatt
    20881 tggaaactgc aacttgtttt tgattcatct gaggaaattt aattttatgt tagcttttct
    20941 tgtttcgctt gtaaaataca tatatttaca taggtttaaa ctttatttta atatattgta
    21001 tacggcgata aagcgatatg ttgtatattg aaacaaatac ggatgctcac caagtttctt
    21061 tttaaactga gtgtaagcat ccatgctttg cattcttctc catattggct tattcgtttc
    21121 tttaccttgc tgacttaatc tgttctaact tttctctaac agatggtgca ttatgcgtaa
    21181 gtttctttat atcgacatcc tcaaccttat tgtttgctgc atttaagtga ctctcggatt
    21241 taagcaaatt ctctctaatt tttgtcaaag tagaaattgt cttatcaata ccatcaattg
    21301 catcaacaaa acggtttttt gcattttcca gactataact aaaactgctt ttaaattgag
    21361 acaaattttc ctcaaaattg cgtagatcaa gttgctgatt ttctactatg gccaatttct
    21421 tctgatattc caaagaattt aatgctgcat tacgaagcag agaaataatc gggatgaaga
    21481 attgaggacg aattacaaac attttggggt agcgataaga gacatctaca ataccgttgt
    21541 tataaaactc gttatccatt tccaggagcg aaaccagaac ggcatattca cagttcttct
    21601 ctgttcgatt tttatccagt tgcttaaaga agctctcgtt tttctgcttt gtttcagttg
    21661 tatccatctc attcttcatt tcgaacatta tggatataaa ctctacttca tcggatgatt
    21721 ctctgaatat gaagtcccct ttgctaccgc ttttagcatc gttatctttt tcaaaatatg
    21781 catttggaaa agcggccatg cgaatctgat taaactgatt catacaatgc tgttctaaac
    21841 tctcgccgac catcttggtt gattgctttg ctttaaaatc ctttaacata ttaatttcgt
    21901 cttctctaag tttcaactcg tccttgtgtt tctcaataag attattcttt tgtaacacgc
    21961 ttgcctgttc ttgtaacttc agtttgcttt cgagattgga tatttcagta atcttttcag
    22021 acaattcctt gtcctttgcc tgaacagcct gtgttactgc aagctctttt tcagtttgag
    22081 catttttcaa ctctttctct ttcatttcgg caagttcctt cttaaactcc atgtcacgca
    22141 cctgtaataa aatctgagca taaccggtct cgtcaatttg gaacattttc ccgcaattgg
    22201 gacactttat ttcttgcata ttaatacctc cttaatctaa tatctctatg ataattcatg
    22261 ttcagaagaa acttcaaatt tttctccatc atatgtccag gttgttgtct ctctacttgt
    22321 tgcactacac aaagagtctc tgaattcttc atttatctca tctgtaatct ctgctccttc
    22381 atcaagttcc atgtgatgtc tgataaagtc atcatcgtaa ttataataaa atctaccttc
    22441 atgctctccg ccccaacccg tattattatg ataccaatct gattttcaaa cacctcaatt
    22501 cattacacag ttgtcgccag caaatccaaa gtaatacata ccttattcac aataatcttc
    22561 tgaatcaata ccgtatcttg ttgctatgga tattagttct tcaggtgaag catcatcaat
    22621 gtcaaatgat tccaccaatg ctcctccata tccgccaaca aaatatgctc cctgagcttc
    22681 agccttcaag gcttctctga gcttttcaat atccagcttt tcacctttgt tatccattgt
    22741 atttcccctc tatttactga tgttatttcg ctatctctga gtcatattta ttataccatc
    22801 attggtgtcc gttataacgg tctgttaaat tgccatgcaa taatgaacat attttaaaca
    22861 cagtcatttt agtgtattat taacatatca agtcaaacac ataacaacag gtcatttcta
    22921 tatctacagg aggaactcat gcaagaacat aatataagac ggacaatacc attgccaaaa
    22981 aatatacgtt atacatcctt tcaattttat gcaaaatctg aaccggggca gggcatttct
    23041 gtggaacagt tatacataaa gcaaatattg tatgtactta actggataca aaaaaggtgc
    23101 ttaaataagg ataactctaa tcatgcggat ttaccggaat ttctgcaaaa tatccctaaa
    23161 cctgaagagt ataaaaatta tccccttgaa gatctagctt cttatagata ttatgcagga
    23221 tttcaaatag agataatggt ttcaagagat aaaaagaatt gggctatgag agtacaagag
    23281 attgaccgag ggccaaaacc cgataagccg gacgaagcta gaaaacctgt accgggacgt
    23341 tcttttataa cagacatagg attgcgaaga aatgaaagta gtgttgaaat cgcaacaaga
    23401 acccttgtag atgaaccgga aggaactaaa gaagagtgtg atgctttcag gcaggctttt
    23461 ataaaagaac ttatagcaga taaggacatc aagctggtaa atgatggttg gcctgtacga
    23521 gaggaacatg ttgaagtaaa aaataaagac aattataaag acttcataaa atggctgaaa
    23581 tcagataaaa gagaagcact agctgttgta tactgtgcat cacctaaagt tataaaggta
    23641 aaaataaaag aagaatctga agataatacc caagtacata ccgagaataa agatgagaca
    23701 aatgttatag attatcttga tgaatttaaa aagctggctt tgtctaacag aggctatgca
    23761 tttttctatc atatactacc ggaaagccgt cgggaaactt tccttaagga tattgctgaa
    23821 attaactttt cgaacaagaa aacaccggtt cttgacaatg aggatttgtt cattattggt
    23881 gccaaaaaat ataaaacatc aactgtatac gcatataaca tgaaccagca ctgcacagat
    23941 gattctacaa aagcgactgt tataaaaacc attcgaaaat attatgaacg aaaagacgta
    24001 agtttttttg gggtccggtt cgtaccggaa ttaaaagaag ttttagatcg ggaaaaagag
    24061 gaaaccaaga aaagtacgtt ggatttactg gaaaaaattg atggattgga acaggaatta
    24121 cattcacaaa aaagtgatta cgagaaaaat atagctgact tgaatagtga aatacgacgg
    24181 attattaatc aaaaagataa ggaaatacag gatctgaagg atgatttgat tcaacaggaa
    24241 aaggatagta aatctttcgt agaaagccaa aatagaattc ttaacgaaaa agactactta
    24301 atagaaagac taagaaatcg caaaaaaaga cctaaaaaat tcgaggatat acctaaatgg
    24361 gttaataata gcttcaaaga cagactcata atgagtgagc gagctcaaaa agaggttaat
    24421 gaaatcacga aagaatacga cttggataat atttgtgatg cgcttgaata ccttgcgttt
    24481 gagtatcgtg atcttctcag aggaaacatt tctgtagata tggtaaataa tagagcctca
    24541 aaaatatacg accgaagtta tgaagttaca aaggtagatg tgcctggcat aaataataaa
    24601 accgaatata gttttaagta ctataagagg gacggaacac cttgtgaaga tgaggaaatt
    24661 gacagacact tatatttgaa taatagtaat cgtatttatt tcatatatga taaagaggat
    24721 aaaagaatag taattgcctc tttaccgcat catttgagaa caaagagtga cagataatca
    24781 cagaatctgc tgattattta tattcatctt aaacttcaga ttaaggcttt ccgcagcttt
    24841 tctgcataat atcattctgt ctaagaatat ctcaaagtat tccaatacag agtaatacct
    24901 gggatctatt gtgagactta aacatatttc cgtcttatct tcatttatcc tgaccgtgga
    24961 tttcttaacg gaatagttta ctctgtcatg tatatcaaaa tgtgccgtat cggtatttct
    25021 tactctgctt cttctgacat cggttttatc tgcaataatc agtgctgcgg caacgcaatt
    25081 gaccggaaaa gcggtactct cgtcatgatt acctatggca gttataaccg tagcaatttc
    25141 atcgggtgtt gcgttcatat cttttagaat tctgaatgcc aacatagcac cgctttgcgc
    25201 atggtcttcc ctgttgataa gattccctat atcatgcaga taacatgcaa tctgtgcaag
    25261 ctctatctcc ttttgggaat accccagtgt tgtcagtata tacttggcgg ttgttgccac
    25321 tttaagtaca tgagcaaaac tgtgctcggt ataacccaaa gctatcagtg attcatcggc
    25381 tttggctatg tatgtcttta cttccgtatt tttcttaaca tcgtcaaatt ttatcatttc
    25441 ttttcccttc tcatttttgc ggctttaaca acatgctttg caagcaccga agtggtaacg
    25501 gtgcctactc ctttgggaac gggagtacat aaagccgcta tatccgatat gtcctctata
    25561 tccacatccc cgcataaccg tccctcgtcg tccacattta tgcctacatc aaaaacatat
    25621 gccccttcgg atacatggtt acggtttatc atccttgcct tacctgcggc agctacaagt
    25681 acttcggcat ttttacatgc cgccggcata tccgccgtcc gggtatggca cacggtaatg
    25741 gttgcattct ttttcatcag catcattgct accggttttc ctattaccat tgagcgccct
    25801 actatcgttg ctcttttacc ggtaagatca attcccgcat aatctatcat ttctattacc
    25861 gcttcggcag tacacggagc aaatcccgta tcatcaccgg aaaacacttt tgccatattt
    25921 atagggctga tacagtcaat atccttaaca ggattaatca tttcggttat ggctttttca
    25981 tccagatgtt tcggaagcgg acgtaaaaga agaataccgt caacatcatt atcgtcattg
    26041 atttttttga atgcaaaagc aaaatcctcg ttatcaatac tctcgggaaa agcgtgtaaa
    26101 gtacatctca aacccagctt ttcaaatctc ttgaccgctc ctctttcata agctaaatca
    26161 tcgggtttct ctcccaccct tacaatagcc agatgcggta tataccccga caatgctgcc
    26221 agttgattcc gtatctgttc cgataaggct tttaccacat cgttaccgta taaaatttcc
    26281 ataatatcac cttaagtttt tctctaccct ttcgaatatt tcatcggcaa gttttttgcc
    26341 tttctccatt atatcatcac aatgactgtt taattgctcc gctctgttac ggtctctcat
    26401 cgctttggta ttaatatata catttaccga tgctccggta atggctgcac gcagcataac
    26461 cgccgaaacc gccacatcgg atatagctat tctggagcct ttttcggcat actcccctaa
    26521 cagtaacagc atctcgtagg atttttccat aatacttact ggaaccgaac aggcggtatt
    26581 caaagcctct tccattactt tgtctttata ctccttttct tcatcggtgg tactcggcaa
    26641 agaataggct ttcgataacg gaagaaaaac ttccgcgtct ttgtctataa gttccataag
    26701 ttcttcagtc agaaccgata ctttttgctg taagattatg atgtcttcct gaacatcggc
    26761 atatcgcttt ttacccagcg taagattacc taccatactt gccaaagccg ttcccattgt
    26821 acctgccaat gcactggctc cgcctccgct gggcactggc tgctttgacg ataaggcatc
    26881 aataaattcc tcaatactct tgtttttaaa catatcctca tccttttttt attgtcatac
    26941 agaaagtata ctatatttac cgtaagaaaa aaagaacgga tacccgttct tcaaaacaca
    27001 ttcttactca attactaata tattaatatg attaggtcag cgttttagat cttgtttact
    27061 tatactaagc aaacattggc tgctctaagc tttttgctgt tcttaggatc ttcctctaca
    27121 tcaaaagtga ctttttgtcc ttctttaaga gtcttgtagc catcaatgtt aatagctgag
    27181 aaatgtacaa atacatcgtc tccgccttcg tcattggata tgaatccata tcctttctcg
    27241 gaattaaacc actttaccgt acctttgttc atctggtcct ccattaaaaa atttacccta
    27301 tacaactaag aaacccttag gtatgaaacc tctaaccgaa atgattttac aagccttgtg
    27361 agttcataga agcatttaga tactgaaatt tataccacag cgctttttac ttgtcaatac
    27421 attttcattc ggacaggaac agagttatct gcagaattca aaattgacat ccttatcgga
    27481 tacatcatgg caagatttcc ggggaaaata aacaggtttt gaatggactg ccattgcaaa
    27541 ttacaacgag acaatggttt atactctttt ggaagttaaa taagcagccg gctatatcac
    27601 tctgtccaat gacggtacaa aaaatattga gaagtatatg ttatcagcaa gtaatcagaa
    27661 cctgagaaga agtagaaaat aaaacgcagt ttttaggtgt tttttaatta taaaaccccg
    27721 taaaatcaag ctttaccgag tttagcattc gtttacatca ggagcgttat gatttcagta
    27781 ataacaagtt aatctttcct tttcatctct aaaatagcct caatgaattg ttccggcaag
    27841 tcagcatgga tattgtggtc ggattcataa cctgacataa gaatactgcc ttcaatcaaa
    27901 gactgcactt ccttcgcatc cttttcatcc attgcagaaa gcaaaatgcc gttttcatca
    27961 taataaccgg gcgcggtctc ttttgatggc gcaacatgca gaatgattgt cggacattca
    28021 atttcactta aagctcctct ttggtcaaat ccctcaaacc aggagaagtc ataaaaggta
    28081 tttccaaacc tcaagtcata catacctgtc ccatcctgca tatttcgtgt gagaaaaacc
    28141 aatccgttca atccgatttc aggaggatag taccaaacaa taggcatttt ttcactgttc
    28201 actttgattc gtttggtgaa tgggtttttt acaaggaaag accagttatc tttgccgtct
    28261 ttattaaaaa cttctttcaa atatgtgtgt tcaagaaaat atttcgtata attcttttca
    28321 tcgctctgta aaagaaaatc atggattgtc ttaaaatcaa gataggaaaa agtcttttcc
    28381 gccctccccg gttctgttga aaaaaacggt ccgtcttcaa gaagcaagcc ttttacctgt
    28441 tctttatttt tcgctgcgat ggcagaacta atgagcgcac ctgaagaatg tccgctcaga
    28501 aaagtctttt ctttaattac atcgtgcatg aaaagaatca tatcatcccg aatggaaaga
    28561 atattatatt ttgtaggatc ttttgatgag tttccgtgac cgtagcaatc caccgcgaaa
    28621 atgtgaaagt gcttggataa ttccggcagt accttggcat agtcctcttt ggaaaccatc
    28681 tgaccgtgaa taagcagtaa cggctctcca ttgtcagggc cttcaaggta cgcaatttca
    28741 ctgccgtctt ttgttttata tatttgttct cgaaacccaa tttcagtacc tttattctcc
    28801 ataaagcgat cgtttccata atcataatga aaatttctat aggcaaagac gattaataat
    28861 gcgatagaaa aaatgaagaa taacatgata aactttagcc atctcataat agtttacctt
    28921 tgattttttt aagcatatct ttcatataat caccgctgac tatagcaata ccctgacttt
    28981 gatctccaag taaaagcagt gtgtcaccac tgtttatttg aaataaatct cttgcctctt
    29041 tgggaatcac aatttgaccc ttactgccta cttttgctgt ccaagcgaac aaaccctgtt
    29101 ccttcttttc ttctgcattt ttaattatca actttgaacg gtttttctga tttattttca
    29161 tatatatcgc tccatcagta taattagtat aaaaagtatg atttgttata ctaattatac
    29221 ttcaatggat tgcatttgtc aattaaagtc cattatctcc attttagtat agaacatgat
    29281 tatactttta ttttctaatc gctctaattt attccatatt caattttaag aaacgagagg
    29341 aaggattgat gtgctgctta gaattatcct aaaaatccgg tcaaaaatcc taaaataact
    29401 ctaagtaata caccaatttt cgaatttcgt actttgcgga agctctgaaa cccttttggt
    29461 tactgcgctt ttccccttta gcattattat gacatactct cctgagaaga agtcgaaaaa
    29521 accactgaac tacggcacta ttttcaagtt caactttaaa ataatgccct ttaatattat
    29581 ttttgccgtt aaatgctcag atatgctctt tcagtgcttc caccgcgtta tatatccata
    29641 tgcccgcatg agctgcaaaa ccgtgattta acgatgcacc gtcggtctta ttctcccaca
    29701 atgtaccggt tctttcagcc atgtacttga aaaatcctac cgattcgttt aacagctgtt
    29761 tatacaaccc ctgttcggtt aacagttcca ttctaagata tgtacctacg aaagcatttg
    29821 caggatatat ttccggatgc aatttctttt ccactctgtc cggtccgaaa tccttgacaa
    29881 gggttttcca taacttcgaa tatttcttct ttgttgccgt ttttgtaaaa aatgcataat
    29941 actggcacac ttccgtgcgt tcgtgagaaa cttcaagcac atcattctta aataccatgc
    30001 ggtcacggaa aaactccccg tcatatgaca gttctcttat cttctttcta ataccctccg
    30061 cctgcaccag tttatctttg tcatcataca gttgtgatac tctttccaac atcaatgcat
    30121 aaagcatatt ggtcgggaaa ttcacatcct gcaccagttt attggcaaaa gaccattcca
    30181 cgaaaaccca tttttccaga ttctgcaaaa gaccgtcttt attcagaaat ctttcgaaat
    30241 attccaacaa agcatatatc ttcggtttga atgcggcaac catatgaata tcattacttc
    30301 tctctttgta ctcatacagt tgcagtacaa accacattgc ccagttgggt atgaagtttt
    30361 tgtttacatg gtcggcagga tagcacatgg gaatcatgcc ttcgggaagg gaaggataag
    30421 attcgggaag aaggaaattt tccaaaaagt tcttctcaat caatgattta cccgtaagga
    30481 aatattccac tctggaggca aagaaactgt cacacagcca ccccgctctt tctcttgaag
    30541 ggcagtccat gaatatgtcc agtgcatttt gtctgtaagt ttccacagcc gcatcaaata
    30601 tttttttaag atcgggatcg gttgtatcaa tagccttatc tacaggcgca tgcttgtatt
    30661 caatcatatg taaagaacag atacgtgccc tacctctgaa cattacgaca tttaagtatt
    30721 tcatactgac cggctcgaaa aataccaaat tgtattcacc cggctccaga acatatttat
    30781 ttacccataa gccgtgtatg cgaatgggat cgggtacccc gtttatgagc atctcatcat
    30841 aaatcatcaa aaactcacag gtttctttgc agacaatacg gaaagatata aagccggtgg
    30901 cttctctttc aaactcataa acggcatatt cgctttcgct taagcaaata ccgttactta
    30961 tatcggtttc tacggcatta gcggtatcat actcaatctg ctgtattctc tcatttatat
    31021 tctcatccaa ctcttccgtt ttatactgct tatagtcagg attatctatt ttcagatgcc
    31081 agggcagtct gtatgtttcg ggtttacaag gtttatgttt acctacgacg gcattatcca
    31141 taggacacaa cttttcatat tcgggataca gtacatttct ggttatatac tccttcttac
    31201 cggacatctc cagacttacc tgatgagtat tcccgccggt catccagtcg cgggaatcgg
    31261 aagttaagac ataagactcc acaaagcccc tctggtatgt gtatctttgt acctttttca
    31321 ggtaatcatc taaaagatat gcaaaaaatc ccttaactcc ggtagctgca accacgtcat
    31381 tatcgcaaac tatttcacag gtcagaaaag acggttgatc caggaaggaa tatgcattta
    31441 tacaataacc ccatactata acggcaacat cgtttatgtc tttgttaaga taagaagtta
    31501 tatcaatctc atccaccctg taatatccgc gtgccgctct tgcaggacct gcagccgcaa
    31561 aagtatcgtt gacatatatc tgataagcgg tactgcaggc aagattaacc gatgttttac
    31621 ctttacctgc ttgtgcgtaa aagcccatcc ttatattcat ttcattttgc agtccggctg
    31681 cccacacggg ctcggcctgt ttaaaaatat atcgctccat atttgcctcc gtgatgtgta
    31741 taatttaaca tattttcgct atgataacaa acggcgccgc ttttcttgac aataaaaact
    31801 ttacaatata ttgtattata taagttgtat actctgtaaa aaacacatta aaaaggttcg
    31861 aaaccatgtc aaagaagata gttatactta tattattcgc tcttattgca tctttattta
    31921 catcctgcaa ttgctcttat gttccggaaa gtgattcaca taatactgaa agctcatttt
    31981 ctataacaag caactccgac aatgaaaaac ctgaagaata ctacacggct ttcagacagg
    32041 gagaatcctc tgtttcatta tatcttgtaa ccccctacgg catgtacatg aatatcagaa
    32101 agtatatcta ttactcggaa aaaggaaata ccgaatatat aaaactgtgc aacaaacccg
    32161 cctgtaatca cagcacggaa gactgtaacg catatacagg cgcttatcaa ataggttatt
    32221 acaaagataa aatatactat atatccagaa gagattatta tgtcaacaca ctgaactgta
    32281 tgaatatgga cggaacaaat cataaagaaa ttaaagtcct gaacaattct tgctttagta
    32341 ctttcggcta tttccataac ggatacttct attatattat cggcactccc ggagttcaag
    32401 caatcggcgt tacaaacgat gacaacaacc tatacagagt taaggttgat gataattcaa
    32461 aaccggaggt tgtacttacg gacgatatta tatttaatat acaaagattc attgttgtag
    32521 atgatattat ctatctgaca gtttataaac aggatacaac agcagaatgc tttttatact
    32581 catactcctg taaaacaggt gcattgacaa agattgcgga caactggtcc ggtaacggtt
    32641 tttggggaaa gcattacgga tattgctatc tgacgaatga agggatatat aagtacaatg
    32701 ttgaaacagg tgaagtcaca ttggaaaagg caattaaatt tgataatcac gggtactgcg
    32761 gagcaagatt ttaccccgac tacatatatt taatacataa tcgcaatgat gattatcgag
    32821 cactgcgtga gcaggacttg gttttgtata tatataattg ggattatgag attatagaga
    32881 ccgttttgtt ggattttatt aataagggca aaataggaaa ttttattaca gatgtcggca
    32941 attacattat atttgcatcc gatatggata ataaaccgga ttactatata gacaaatccg
    33001 agataggaac cgataagttt gcatttcata aaatagagaa ttagttatgg caggtaatga
    33061 aaggagacca actatgaaaa gtcaacagag aaataacgaa tattgatgat tcat
//
LOCUS       DAOSOM010000022        32289 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_1412_length_32289_cov_6.577248, whole genome shotgun sequence.
ACCESSION   DAOSOM010000022 DAOSOM010000000
VERSION     DAOSOM010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 32289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32289
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1412_length_32289_cov_6.577248"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="PLF14_06335"
     CDS             <1..376
                     /locus_tag="PLF14_06335"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT70944.1"
                     /translation="LRRISDKCHEYGIYHVFGSDGYFWDVADDFYIHSGIDGHYEADC
                     SCGMDIRSVRTKYPDITVIGGISAATLDSKSQDDVRTEVTEAVLAAKELNGAIVGCSN
                     LVSPTTPIRNFMLMMELLHKYK"
     gene            complement(390..3191)
                     /locus_tag="PLF14_06340"
     CDS             complement(390..3191)
                     /locus_tag="PLF14_06340"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOT70945.1"
                     /translation="MDIQASKKGISLKLNHRRIIFFLLALLFLTGGVFFTHRICNDHF
                     DIQKDEALLLADSSSSLIPQSYLKALDNNISDLSKKEYISIKNSLIQLVKTNDHIGYA
                     YFVMMQKGIIVLAVDSSPADSEYYYLPGQICSGAEKLKHIRFNWQTPQISKPYENDSG
                     RWISVTVPVTDNETGEILALFGLDYPANQWYIAQRKIAIQSGIFWFGTIAMLAVIYMI
                     SSINTQLKKDRQLIKETDDRLNESMTLYKGVFDQTAIGIAIMDGYNYLVETESGLPGI
                     NAAFENILGRKKEEIATTTWVDLTNPEDLDEDMEYFRKFKSGLIDRYDMEKRYIKPDG
                     EKVWVHMTVSPLYFENVGNKYHLCTIRDISKRKQIEKNLLESERSYSLLLDNTPGMIY
                     RCKYDRDWTMLFVSQGCFELTGYHPESLINNAVKSYNDIIVPEYREMVWQKWSYALSY
                     KQKMVYEYEIITASGETKWVCEQGQGIYDELGNVIALEGLITDISARKEHEETIKYIN
                     NHYPVTGLFNQKYLIEKFSGDIAAKDHSSKALLVIRIRKYNLILTTYGYSYCRSVIKN
                     IATHLTEITDDSHILCQLSMDKFALYVKNFRTKNELVKLCDRIIKMLSSNTALKAYGA
                     WVGVDIFNKKDKDIQTVLKNATMTATLTDAVKPFGYSFYDTDMESDVMRQEYIKDILA
                     RICDNTERDSFYLEYQPIINRDGSIHAFEALARIKDKELGNISPIEFIDVAKKRYLIV
                     ELGRIILDEACGFIKKLQDNGWPDVSVAVNISEIQLSRDDFLTDLKNAIDKNSVLPEN
                     LILEIAESGFADNLEMINNQLTKAQILGIRFAIDDFGTGYSSLAKEHEIAADIIKIDK
                     FFIDQLLVKDEKELITSDIINLAHKMGQKTVAECIEYPKQLEYLKRNNCDYYQGFLFS
                     KPVAAQKALELLESGKFSI"
     gene            3402..4307
                     /gene="pfkB"
                     /locus_tag="PLF14_06345"
     CDS             3402..4307
                     /gene="pfkB"
                     /locus_tag="PLF14_06345"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008400087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HOT70946.1"
                     /translation="MIYTVTMNPAVDYTVLVPQLALGEVNRTEREAYFFGGKGINVSV
                     VLHNLGIESIALGFISGFTGQFIRQGMQSLGIKNDFIELSSGMTRINVKIKSDKETDI
                     NGIGPSVDRDSVAALLNKTKALKDGDCLVLAGAVPKNLDTKIYSDVINSITAKDIYVI
                     VDATEDYLLNTLKHKPFMVKPNRAELGQLFGKEIHTDSDIVFYANKLQKMGARNVLVS
                     IGNKGAVLVTENNRIICRDAPKGQVKNTVGAGDSLTAGFIAGYMHSADFDYALRYGLA
                     AGSATAFSETLATKEKIEQVLNGFE"
     gene            4439..7012
                     /locus_tag="PLF14_06350"
     CDS             4439..7012
                     /locus_tag="PLF14_06350"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOT70947.1"
                     /translation="MANKKKSTKNTKKDKKNIAVLVAITVILCIAISFIYAYMKGWFD
                     GEITPTPSLIRTEEPTIVALPSPSGTVTDTPSPTPTGTPVIEQPLPENHIKIEKFEDL
                     LGYFEYLLSVKTGVYALSVQDITADKSFDISASRKFDNVCAYKLAVVMYAYEPEVRQA
                     MPLDTSVEYTEADRDGNSSVVAKGNYSDTYTIQKLCEYVLKSDDRAALNMLLRVYGKN
                     NIEKYMSDRGMSGSESYYSPDDMTVGFKLFKDKIDSHVDGYSAIREYLSSEILTGVSS
                     VSAYTKDSDIFSMNYTAMVYAKNIFIVSMSAKDANLSEAESTFKDICGIINTLFAENA
                     VIVEKSKYSHALILDNIDEHARQTAANLQSVVMYPDGTASNVYISEDDISFPYSEGYS
                     DIGITTYRGNNYRDSGFFGSISIKERKLEEIWSYEIGGMTGAGSIWPGVGWTGQPCIV
                     KWDREMLSIMNVYDKFKSRNELIEVIYATLDGNIYFLDLHTGEPTRNYIYIGVPVKGT
                     LSVDPRGYPLLYVGQGLEAAKTDEGEDRYRPFGYRIFSLIDNKLLHEIKGYDTFAPRP
                     GWGAFDSSGILDIKNDTYYQCGENGIIYIVKLNTVFDKGAKTIFINPETIKYKYVSES
                     AYSYGIESSPSIYEKYMYFTDNAGMLICLDLESLKILWAYNTGDDTDASVVLDVTEEG
                     VFLYTANEVDIRGRDDNSNIRKFNALTGELLWQVDVPVFYVDVINGGALASPVIGKGP
                     IENLVIFNICKTLEDSIWAGRLIAIDKLTGQIVWIYKSPYSWSSPVAVYTEDNKAYIV
                     YCEHNGKISLLDALTGKQIDLVTLSLGRQYITIEATPAIYDDILVVGTYAEKIYGVRI
                     K"
     gene            7014..7244
                     /locus_tag="PLF14_06355"
     CDS             7014..7244
                     /locus_tag="PLF14_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004930 - G protein-coupled receptor
                     activity [Evidence IEA];
                     GO_process: GO:0007186 - G protein-coupled receptor
                     signaling pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7 transmembrane receptor"
                     /protein_id="HOT70948.1"
                     /translation="MKRIKSNTTVAVVISGVALVLAIANIILCYVRNLEKSVSMIILI
                     CIALVFAYNLAYHISAKKKAKREAEREEDIDK"
     gene            7292..7534
                     /locus_tag="PLF14_06360"
     CDS             7292..7534
                     /locus_tag="PLF14_06360"
                     /inference="COORDINATES: protein motif:HMM:NF014080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT70949.1"
                     /translation="MRSFCSRGYKVVLPVSEEKNNLTEDEKRVYSLLKRRRLSSSEIV
                     KATGFGKTKAVSILNNLVAGGYIETTGKGRGIKYFA"
     gene            7648..8778
                     /locus_tag="PLF14_06365"
     CDS             7648..8778
                     /locus_tag="PLF14_06365"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT70950.1"
                     /translation="MFNDMKIVDCHIHYSSNIPVEEMIRILDYCDTDIANIVVVPDRA
                     KLSAVQDALILKHRLSDRIYVFASLDASNYFIKKKTLGKAMAKYALRMREAGCDGIKM
                     IEGKPQIRKMLNVPPFDESVWEPYFAFMEDTAFPILWHVNDPEEFWDKDKIPKWAMQQ
                     DWLYDETYVNNEKQYEEVLNVLKKHPDLKIIFAHFFFLSKQLDRLSEILDKYQNVYVD
                     LTPGIEMYINFSENKEKATAFFEKYQDRIMYGTDIGTKGVVDGKSASLEEEKKRADLV
                     KRYVSSKADFIIDKHYSYLMHDNDFTLSPLGLSKKIQKKIFSLNFLDFMGHTPYPVDS
                     NKVLREIRKTVVKLCLMKIGDKSGTEYDFASLKKAYKQLEKI"
     gene            8788..10161
                     /locus_tag="PLF14_06370"
     CDS             8788..10161
                     /locus_tag="PLF14_06370"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT70951.1"
                     /translation="MQKIKRNYLPLYALSGFGPGLINLMISAYLIDGLSTAGFLKNIE
                     NWTYANKTIVMTALFSVFVLIAKIVDGVADVPLASLTDTLKTRWGKRRPAILIGYIPM
                     IITYILFCFPLEIAEHSLLNTIWFGVLLIFFFTSYTMTLVTYYGTYSEVTENDSDRHY
                     LSSWKAFFDTVQYSIGYAVIPLMVGGLNIRHIALILSPLLLTMIIPFFLLKEQSTLPE
                     DVAKRVEAGEAVEEEVPFMESIKLTIMNKGFIMWLVVFGIFFFGLQMFLAGQNVLASG
                     PMGLNSWQIAIINSAAFAPVPLMLYFYRKVMKKKGFRFAFQTAMGSFAVAMLMFSIAY
                     IKWIPDEYIRLAIGATGATIGSYGLGAFFSAPYAIPAQAAADELKATGKSHPSMYFAM
                     QGLCTALVGALSTSVVWLNIKEITLPDNPVFGAHLMPYIVIAACVTAIIAAKYMPKEY
                     NEMGKEK"
     gene            10158..11057
                     /locus_tag="PLF14_06375"
     CDS             10158..11057
                     /locus_tag="PLF14_06375"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOT70952.1"
                     /translation="MSAYDNTGSGRLYLAIDIGGSSIKYGVLDESFSFVHKSKIPSRI
                     FLGGEELLNRVMGIIADMLKQYPLEGVAISTTGAVDSSNGRVLYADDVMVGYTGTDFR
                     EIIKQKFNLSATACNDVNCLALSQIQFNKGKDFIVVAIGTGIGGGVVISDKLYEGYGY
                     SAGEFGLMHLRDGKNWESLCSISALVKTAKEHKLLVSNGRELFDLFDADNDTAYRIID
                     AFYKDMASALANLIYAFSPQKIIIGGGISARGDSLVAGFKRYLSMMTDQFYYEKTAIE
                     CAVYENDASMIGALQHYFIINNE"
     gene            complement(11120..12781)
                     /locus_tag="PLF14_06380"
     CDS             complement(11120..12781)
                     /locus_tag="PLF14_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70953.1"
                     /translation="MKYTLDLYKASLKSYEIYLERLIRNQMTQASGWRKDGVIIVDEG
                     FVPTHIAGVLYAPGLLYFNEDSKYYKSKKAMEFAFSVLEFFERNLSPEGLMDFYMCNF
                     RSAPDTSFSIQPLVDLYNIIKDKKTDETGELFKKRLYGFIVKIADGVFGGGFHTPNHR
                     WVITSALAMVMNMTGDKKYLKRINEYLAESIDCDEEGEYSERSSGGYNFVNNEALLII
                     AQELDKPELLEFVRRNLMMMTYYIHTDFLIFTQNSTRQDKGKENIYLDKYLYQYLKAG
                     YLLKDEQLLGVAKAIQNELFSKGRGYPIGLATMMAEPEVLIIPEHIKPFEIQPYVKHF
                     KKSKILRIHHNGMTLSVIEDRETFLYLKYKSFDMFIQGGILFFNERHIKVNNIRPIEN
                     GFAMDYHGQGIYYHPFGQYQGTADFWEMDKSKRATSELLTVDAVILVTLTEKGFRISL
                     KARGIEKVSVRLDIAINNNAFIMGDGYTIKANAGGILLPESGAVRAEFGTCGVKIGPV
                     RNDTYITQGLFGSEPLDTDKYHIFFNYLTPFEHSFTIEPVTKFED"
     gene            complement(12901..13170)
                     /locus_tag="PLF14_06385"
     CDS             complement(12901..13170)
                     /locus_tag="PLF14_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007157176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOT70954.1"
                     /translation="MTYSIEVTSRFEREFKKLDRYTKLMINAWIEKNLLDCENLGTCK
                     KTLIENRKGLWRCRIGDYRLICHIEDKKFIILALNIGHRSEVYKS"
     gene            complement(13167..13388)
                     /locus_tag="PLF14_06390"
     CDS             complement(13167..13388)
                     /locus_tag="PLF14_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006626625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="HOT70955.1"
                     /translation="MAFSIRLTEEERALAESYAKLHSYTLAEAFKRALFEKIEDQYDI
                     AIYDAAYKEYEDSGKQSRPIKEFWKELQT"
     gene            complement(13571..15274)
                     /locus_tag="PLF14_06395"
     CDS             complement(13571..15274)
                     /locus_tag="PLF14_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019421499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOT70956.1"
                     /translation="MAEIPRKEHPRPQMYRKSWINLNGKWQFEIDHGRSGKERELYKD
                     AKLSSSIIVPFCPESALSGVNNKDFMECVWYKRTVRINKSWLENGRRTLLHIDACDYL
                     TEVWINAQSVGTHMGGYSSFSFDITEQLKEGENTITISATDLLRTMEQPAGKQSVGYF
                     SRGCHYTRTTGIWQTVWLENLPSSSIASFKCIPDINTSSIYFDVLCRNANGKKIKATA
                     YFDNKKMASCTAIIEGKHAKFNMKLKELHLWCCETPNLYTLKLELDEDKVDSYFGMRS
                     IYYHDNKFYLNGKVVFQRLILDQGFYPDGIYTAPTDEELKNDIIRSQAMGFNGARLHE
                     KVFEPRFLYHCDVLGYLVWEEHANWGLDVSKDKAWERFVPEWLEIMDRDFNHPSIIGW
                     CPFNETQKDQNPDLIKFVADMTRATDPTRPVIDTSGWTHVSGASDIRDKHDYDQNPVT
                     FKERYAELDKQRKNYDGYVDDNCAPVFISEYGGIWWSETDMTGWGYGERPKSLDEVIE
                     RYGALTEVLLNNPNIGAFCYTQLTDVEQEQNGLYTYDRKPKMDPAVIRAINTKKAAVE
                     D"
     gene            15404..16171
                     /locus_tag="PLF14_06400"
     CDS             15404..16171
                     /locus_tag="PLF14_06400"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOT70957.1"
                     /translation="MKIHNVRHEWPEKAGFILNRPHGPKEYIFLHYFNPVDLLCNNGI
                     IRTNPHACIIVSPDKAQWFRSEQKLVHDWFHFTGDVESNMQKYNLTPDTVYYPYDSLE
                     ITGTVRKMESEFFTDMQYSGKLCELYIEQIFIMMARSEKDSINAANVKKQISDKVEQV
                     RRKMFTSPAEDWPVSKMAKLAGLSESRFYTVYKSVYKITPNRDLIIARIEKARTLLQT
                     KDYSVNQVAEMLGYSSVYHFIRQFKSVTGVTPGSVRK"
     gene            16301..17017
                     /locus_tag="PLF14_06405"
     CDS             16301..17017
                     /locus_tag="PLF14_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70958.1"
                     /translation="MKYYSRALIIVIILATIFVIGMAIEFDHSNIIIKSDSLKPFQEM
                     RVSNGINPCEYKAKVILNNDFVMPQTLLFKTSHLRFKIYINEKMVYSFGYEENAVSFL
                     KSPGTSYHLVELPAMSSNKEIVFDFQSPYSNYHGFLPDIQYGTKGECIAYYQNKSMYS
                     LFSLTVVLLGGLTIMILYFVTKNKKYALSKSFLYISSFIFLVSIWLFFQSDSCQLMFG
                     YPQIMYFADLISLVYCFPFL"
     gene            17062..17904
                     /locus_tag="PLF14_06410"
     CDS             17062..17904
                     /locus_tag="PLF14_06410"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOT70959.1"
                     /translation="MLYFAYAYAATLVLNLLLQVFGLVDMFQLVLLTHSLMALNMIVA
                     VYVVHVELRIEKNKDLRMFIVPLALVSAGGLLEMILYYYSNMTQTSFALRIAVVAFLV
                     VIIINAIKSYYDNLLELKQAEYYEQLASVDMLTNLGNRNKYEQFLQSHEPNQPLTAVL
                     FDLNYLKYINDNFGHVVGDSALKKCAECIKEAFSNKGECFRIGGDEFAVLTKADDNLQ
                     KECNIFEKLVQGWNKRLSYIFSVAYGVARFNPKTDQSAYDTAKRADFAMYHMKEEQKR
                     KIIL"
     gene            18029..18385
                     /locus_tag="PLF14_06415"
     CDS             18029..18385
                     /locus_tag="PLF14_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOT70960.1"
                     /translation="MKEQIDICEYINMTEEEVKEVLKSIPSEDKLYNLSDLFKVFGDK
                     TRIKILFALSVAEMCVCDIASLTRMTQSAISHQLKTLRASKLVKYRKEGKATFYSLAD
                     DHVRTILQQGKEHIEE"
     gene            18389..18595
                     /locus_tag="PLF14_06420"
     CDS             18389..18595
                     /locus_tag="PLF14_06420"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOT70961.1"
                     /translation="MKRTYKLNNLDCAACADKIERKISKLTGVKQVNLNFFTKKAAIE
                     TDSEEIFCECEKILKKYAGAFEIE"
     gene            18592..20436
                     /locus_tag="PLF14_06425"
     CDS             18592..20436
                     /locus_tag="PLF14_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006874634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOT70962.1"
                     /translation="MKKLNKKQKTTLIRMGISVALLAPALLIQNRVISLILYILSYLV
                     IGYEILIKSVKNIIRGDIFDENFLMTIATIGALVIKQYPEAVAVMLFYEIGEFFQEYA
                     VEKSRKSISDLMEIRPEHANLITNEGIITVQPETVKAGDIIVVKPGERVPLDGIVIEG
                     HSALDMSSLTGESLPADINPDDTVLSGSINTFGMLKLQVEKPYNQSTVAGILDLVENA
                     SSRKAKTERIITRFSRFYTPVVVICAVLLAIIPPLLFRQRLTDWIYRALVFLVVSCPC
                     ALVLSVPLSFFSALGSASKNGILVKGGNCLEALASTNSVIFDKTGTLTKGIFKVTAIH
                     PEKLNSQQLLDIAATAESYSDHPISKSLKAEYAKQIENGRISEIKEIAGQGIKAVIDG
                     RKVSVGNENLMEADGVEWHRCNLAGTIVHVAVDDEYMGHIVISDEIKKDAPQTVNLLK
                     ELGITDISMLTGDNKSAAYHIADKAGIENVAHSLLPHQKAEHVNNKPKDKGAVVFVGD
                     GINDAPVLAQADIGISMGLSGSQAAIEASDVVIMDDKPSKVPLLIRLSKKTMRIAKQN
                     MVFAISVKLIVLIAGAAGLASLWIAVFADVGVSFLAVLNAMRLLKKKV"
     gene            20939..21160
                     /locus_tag="PLF14_06430"
     CDS             20939..21160
                     /locus_tag="PLF14_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70963.1"
                     /translation="MDKNIWQPYLQTRAEYLPEDKSVTMATDLKQMNDTELSVLQKRL
                     VNFEDFPIEKHLLRLVFRIIWKFYVTQKS"
     gene            21136..21192
                     /locus_tag="PLF14_06435"
                     /pseudo
     CDS             21136..21192
                     /locus_tag="PLF14_06435"
                     /inference="COORDINATES: protein motif:HMM:NF036995.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21455..21673
                     /locus_tag="PLF14_06440"
                     /pseudo
     CDS             21455..21673
                     /locus_tag="PLF14_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611963.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
     gene            21791..22684
                     /locus_tag="PLF14_06445"
     CDS             21791..22684
                     /locus_tag="PLF14_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HOT70964.1"
                     /translation="MADDKLPVIVENREIQDMIYAIRGKQVMVDSDLADLYQVATKRL
                     NEQVNRNKGRFPPQFMFQLTEDEYENLKSQIATSSDGSKHGGRRYMPYVFTEQGIAML
                     SAVLRSDVAVQVSIKIMNAFVEMRRFLVSNAGIFERLNRVELKQLETDQKLEKVFNYI
                     ATQTEVKQNIFYDGQIYDAFSFIVDLIKKARNKLILIDNYVDTNTLNLLCKKNQGVSV
                     FIAGSGNGSLTSKDISKFNAQYQGLTVKTRKDFHDRFLIIDDLEVYHIGASIKDAGKK
                     SFGISKIEDEVLKDNLIKKVK"
     gene            22967..23086
                     /locus_tag="PLF14_06450"
     CDS             22967..23086
                     /locus_tag="PLF14_06450"
                     /inference="COORDINATES: protein motif:HMM:NF022018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HOT70965.1"
                     /translation="MVDSDLAELYQVPRGNLNKAMMRNVDRFPERYFFQPINE"
     gene            23580..24827
                     /locus_tag="PLF14_06455"
     CDS             23580..24827
                     /locus_tag="PLF14_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOT70966.1"
                     /translation="MNNMTLKNVQRFDMVKEIRVIRWYLRLLTWIISFPAVWFQGTKI
                     RKQGVKGIKGAYLMLCNHNAFFDFMVATAAIFPRRANYVVAIDGYIKREWLLRSVGCI
                     CKRKFTNDPILIRHLIRIAKKGEIIALYPEARYSLCGTNAVLPQSLAKLVKHLNIPVV
                     TLITKGHHINSPFWNLEKRGNRTEADLKLIISKDEIGKMSVEEIDELIHKEFIYDDFK
                     WQYDNKVRVKYKKRAEGLHKVLYQCPNCNTEYMMGSEGAEIFCKQCNKRWYMTEYGRL
                     EAKEGNTEFAHIPDWYEWERAQVRKQIDDGTYYFDKQVRIDSLPNARGYIDLGKGRLI
                     HNMEGFTLTGMSDGEEFMIEWPANRLYSCHIEYDYLGKYGDCVDLNTMNDTLYIYPEG
                     EDFAVTKLSLATEELYKKLNDMD"
     gene            24974..25384
                     /locus_tag="PLF14_06460"
     CDS             24974..25384
                     /locus_tag="PLF14_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70967.1"
                     /translation="MEIKLESLISYEKFKNDIDNVLEMVEKNGQAVILKDNEPLYIIL
                     KSDRYSILKERTLSKKVNKMTLQEAMKIVLKEVKGRKLHAAELSDEIYRRGLYFKKDG
                     TQARYNQIRSRCGHYPDMFEALPGNIIYLKEGVE"
     gene            25393..26814
                     /locus_tag="PLF14_06465"
     CDS             25393..26814
                     /locus_tag="PLF14_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011498651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HOT70968.1"
                     /translation="MRIEYIRQIIKKGESIDIEFKESKSNISKDVYESVCAFLNRNGG
                     HIFLGVKDNGDIVGVDKVAIGRIKKDFVVTLNNPQTLNPTFYLAIEEIEIDGKTILYI
                     NVPESSQVHRCKGRIFDRNEDGDFDITDNTNLVSGLYRRKQSTYDENRIFPYAGMEDL
                     DLELFEKVRRLVRSLDSNHPWISMSNEEILRSAGLYLMDPATGNKGITLGGILLLGKE
                     ELIHAALPHHKTDAIVRKVNLDRYDDRDDIRVNLLKSYERLKQFVAKHIDDKFYLEGD
                     QRISVRDKIFREAITNLLIHRDFANPFPAKFIIERDRVYIENSNRPHGNGIIDPENFS
                     PFPKNPTIAKFFKEIGWVDELGSGVRKIFKYSKVYSDTKPQFIEGDIFKTIIYVDPLI
                     SIINDSDVSGEEQRIQMIIEYCKVPRSRREIQDYIGIKDRKDFTKRLLNLLIEKGLLR
                     RTFPDKPTSPNQKYVAEEYTEAE"
     gene            27412..28005
                     /locus_tag="PLF14_06470"
     CDS             27412..28005
                     /locus_tag="PLF14_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive phage infection protein"
                     /protein_id="HOT70969.1"
                     /translation="MDTEYVVKQLLQENDGWLTLKEVLEQGISKYEFYKFAEKSGLER
                     AGQGIYVSADVWPDSMYLIHLRSAQAIFSHETALFLHDMTDREPSYYSVTVRTGYNPH
                     RLKEDGIKVYTIRKELHELGKSNIKTPFGHIVPVYDIERTLCDILRSRNGVEIQVFQD
                     ALKSYAKRRDKNLRFLMQYAEALNVSAILKPYLEVLL"
     gene            28005..28832
                     /locus_tag="PLF14_06475"
     CDS             28005..28832
                     /locus_tag="PLF14_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOT70970.1"
                     /translation="MIKTSRQLKDKIRNLAKEKSADAQILMRTYMMERLLERISISEY
                     KKNFILKGGLLIASLVGLDFRSTMDVDTTIKGFDVNLTSVEQMIQNLIDVPLEDGVLF
                     QIKNIDSIMEEAEYPCIRVSMQALFDGTITPLKIDISTGDVITPDAIHYSYKLMFEDR
                     YIQLLSYNLETVLAEKLETIMRRHLTNTRMRDFYDVYILKKLYESEISKPLLQDALDA
                     TVQIRGSTELLKHAEPSLKEIENSDDMRKLWLSYQSHYEYAQNISWDAVMEAVKATY"
     gene            29155..30516
                     /locus_tag="PLF14_06480"
     CDS             29155..30516
                     /locus_tag="PLF14_06480"
                     /inference="COORDINATES: protein motif:HMM:NF024817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOT70971.1"
                     /translation="MKKYNAFVEKLFWLMIIINPFLDAATGIYKTFFEKEDATFSLSP
                     SLIIRLLFLLLLVIYLFTTKNRKAILTLIPIGIAAVASVGVEVVLKMEFSIIEDMMYI
                     ARFAYNVVMLFCWYNILSVLGYEKEQLLKKLNKLLCWTLIIIGVNVIFGQITGTGHYT
                     YADRFGYRGSMGYYIAGNDITAVLMLVFPLSISAFMHTDKTNKKERNFYLVASSMTVV
                     TNSLIGTKTSFLALFTTCAVVIVYGTVILVKKKESLYLKKFGVIILSSLILAAVIIGL
                     EFALSKASFMETISQSLRSTGIILRREGLQTALLSGRQVKLKEAFMQFKEAGPLAWVF
                     GIGRGSQEFVIEMDVLEVLCYYGIFGAVAMLWIYVASGYMLIKNFLKNVNFDTVMAVT
                     AIVLCTGYMLIAGHILFSVTSGYYYTMMFAYADILHIDDIRDLPERIPFPFKKLLERK
                     SRV"
     gene            30701..>32289
                     /locus_tag="PLF14_06485"
     CDS             30701..>32289
                     /locus_tag="PLF14_06485"
                     /inference="COORDINATES: protein motif:HMM:NF020730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOT70972.1"
                     /translation="MRKTKKLLAVILAVSMIAVLFTAVPTSAAELTDPQKLAAMNLFR
                     GDTAEGLTATYLAKAPDRKTAARLLLRFTGLEEASDAYTGTATFPDATSATVYWQPMI
                     GYLKANPGAGFGGYEDGTFRPNDPMTAQAFYKVLLTILGYKQDTDFTWAQTITFAASK
                     GLSKIAGKATLTNADAATAMVEALNANTKSGEKLINALINDEIVTPTQAAAAGFTVIT
                     SITGYEAQYTMGYGGANPLPATVTAVYKDGTTASVAVAWSAFSTTVEGTYNVTGTIEG
                     YGTITTKVTLTKTLTVTDAYPSSTKEITVMFNMPVPTSAAVSCKFGVSVLQGLTQTWS
                     NDRKMLKISRTYNFTPGTYSITALNSTKEFVIENEKLVQVEIGAINLYPQPGAQDLNV
                     TFYNQYGLPMNPLTMSLQLSAFSPQKPTVVFAMNKTATSVTISGVENLEVDNTVNVFV
                     VDNNTANAATKVITVAAAPTITAFSFGELTIAGSATRILTGTSGHLISVNAYDQYGNS
                     YKLRTGNQDISTVVAPTIPLLL"
BASE COUNT        10051 a         5843 c         6893 g         9502 t
ORIGIN      
        1 cttaagaagg atatcggata agtgtcacga atacgggatt tatcatgtct tcggttcgga
       61 cggttacttc tgggatgtgg cggatgattt ttatatccac agcggtatag acggacatta
      121 cgaagccgac tgttcctgcg gaatggacat ccgctcggta aggacaaaat atcccgatat
      181 aacggtaata gggggaatat cggcagctac tttagacagt aaaagccaag acgatgtcag
      241 aacggaggtt accgaagccg ttttggcggc aaaggagttg aacggtgcaa tagtgggatg
      301 ttccaatctt gtttcgccaa ctactccgat aagaaacttc atgctgatga tggagcttct
      361 tcataagtat aaataatgtt gtattcacat taaatactaa actttccgct ttcaagaagt
      421 tcaagggctt tttgagctgc taccggcttg gagaacagaa aaccctgata atagtcacag
      481 ttgtttctct ttaggtattc cagttgtttc gggtactcaa tacattcggc tacagttttt
      541 tgtcccatct tatgggcaag atttataata tctgaagtta tgagttcttt ttcatcttta
      601 acaagaagct ggtctatgaa gaatttgtct atcttgatta tatcggcggc aatttcatgt
      661 tcctttgcca atgaggagta acctgtaccg aaatcatcta tggcaaaccg tatgcctaaa
      721 atctgggctt ttgtaagttg attgtttatc atttccagat tatcggcaaa acccgattcg
      781 gcaatctcca gaatgaggtt ttccggcaaa acggaatttt tatctatggc attttttaag
      841 tcggtaagga aatcatctct tgataactga atttccgaaa tgttaacggc aaccgatacg
      901 tcgggccaac cgttgtcctg cagttttttt atgaatccgc atgcttcgtc gagtattatt
      961 ctacccagct ccacgataag ataccgcttt ttcgcaacat ctatgaactc aatcggactt
     1021 atgttgccca gttctttgtc ttttatacgt gcaagtgctt cgaaagcatg aatgcttccg
     1081 tctctgttta ttataggttg gtactccaga taaaaagagt ctctttcggt attgtcgcaa
     1141 atgcgtgcca atatgtcttt tatgtattcc tgtctcatta catcggattc catatccgtg
     1201 tcgtagaagc tgtaaccgaa cggtttaacg gcatcggtca atgttgcggt cattgtggca
     1261 tttttcagta ccgtctgaat atctttgtct tttttattga aaatatcgac acctacccat
     1321 gctccgtatg ctttcagagc cgtattgctg ctcaacattt ttattatcct gtcacacagt
     1381 ttcacaagtt catttttagt gcggaagttt ttcacatata gagcgaattt atccattgaa
     1441 agctgacaga gtatatggga atcatcggtt atctctgtta aatgtgtggc gatgtttttt
     1501 ataaccgatc tgcaatagga atatccgtaa gtagtcagta taagattata ttttcttatg
     1561 cgaataacca atagagcctt ggaagaatgg tcttttgctg ctatatcccc gctgaatttt
     1621 tcaataagat atttctgatt aaacagtccg gtcaccgggt aatggttgtt gatgtacttt
     1681 attgtctctt catgttcttt tcgggctgat atatccgtta tgagaccttc aagagcaata
     1741 acattgccaa gttcatcata aatgccttga ccttgttcac atacccattt tgtctcaccc
     1801 gatgcggtta taatctcata ctcatatacc attttttgct tgtaggacag tgcataagac
     1861 catttctgcc ataccatctc acgatactcg ggtacaatga tatcattata tgacttgacg
     1921 gcattgttaa tcagtgattc ggggtgataa ccggtcagct caaaacaccc ttgagataca
     1981 aacaacatgg tccagtctct gtcatattta catctgtaaa tcattccggg tgtattgtcc
     2041 aataatagag aatagcttct ttccgactcc aaaagatttt tttcaatctg ctttcttttt
     2101 gatatatctc ttattgtgca cagatggtac ttattgccca cattctcgaa atacagagga
     2161 gaaacggtca tatgtaccca tactttctcc ccgtcgggtt taatatatct tttttccata
     2221 tcatacctgt ctataagacc ggatttgaat tttcggaaat actccatgtc ctcatcaaga
     2281 tcttccggat ttgtaaggtc aacccatgtg gttgtcgcta tttcttcctt ttttcttccc
     2341 agtatattct caaaggcggc attaataccc ggcagtcccg attccgtttc aaccagataa
     2401 ttataaccgt ccatgatggc tattccaatg gcggtctggt caaatactcc tttgtacagg
     2461 gtcatgcttt cattcaagcg gtcatccgtt tcctttatca gctgacggtc ttttttaagt
     2521 tgagtgttta ttgatgaaat catataaatc actgcaagca tggcaattgt accgaaccag
     2581 aaaatacccg actgtatggc aatttttctt tgtgcaatat accattgatt tgcaggataa
     2641 tccagtccga ataaagccag tatttcaccg gtttcattat ctgttacggg cactgtaacg
     2701 cttatccacc tgcccgaatc gttttcatac ggcttggata tttgcggggt ttgccaatta
     2761 aatctgatat gcttcagttt ttccgctcct gagcatatct gtcccggaag ataataatat
     2821 tcggagtccg caggggagga atccaccgcc aggacaataa tccctttttg catcattaca
     2881 aagtatgcat atcctatatg gtcgtttgtt ttgacaagtt gtatcaggct gttttttatg
     2941 ctgatatact cttttttgct caaatcgcta atattattat ccaaggcttt cagataggat
     3001 tgcggtatca gcgaggagga actgtcggct aaaagaagcg cttcgtcctt ttgtatgtca
     3061 aaatggtcat tgcatatcct atgggtaaaa aacacaccgc ccgttaaaaa cagtaatgca
     3121 agcagaaaga agataattct gcgatgattg agtttcaaag atattccttt tttcgatgct
     3181 tgaatatcca tatgtgtcct tcaaataaca actttactga taatatattg cttaaccgac
     3241 cttgtaaaca agggtatcac attacatgtg attatacaat aatatagcaa aaaaatgcag
     3301 agaataaaat aaaaaaatgt cttcagacat tgcgttaagc gatttttcaa agcaaattaa
     3361 catgatatac ttatgttgtc tatatagatg gaggtgttgt cttgatttac acggttacaa
     3421 tgaatccggc ggtagattat actgtactgg taccgcaact ggctttggga gaagttaaca
     3481 gaacggaaag agaagcatat ttcttcggcg ggaaaggcat caatgtatcc gttgtgctgc
     3541 acaatctggg gatagaaagt atcgcattgg gttttatttc gggttttacc ggacagttta
     3601 tacggcaggg aatgcaatct ttgggaataa agaacgattt tatcgaactt tcttcgggta
     3661 tgacaagaat caacgtaaag attaaatccg ataaggaaac ggatattaac ggcattggac
     3721 cttcggttga ccgggattcc gttgcagctc ttctgaataa gacaaaagca ctgaaagacg
     3781 gtgattgcct tgtcttggcg ggagcagtgc ctaaaaatct ggatacaaag atttattccg
     3841 atgttataaa cagtataacg gccaaggaca tatatgtaat tgtagatgcg acggaagatt
     3901 atcttctgaa cacgctgaaa cacaaaccgt ttatggtcaa gcccaacaga gctgaattgg
     3961 gtcagctttt cggtaaagag atacataccg attcggatat agtattttat gcaaataaac
     4021 tgcagaagat gggtgcaagg aatgtgcttg tttccatagg caataaggga gcagtgctgg
     4081 ttaccgagaa taaccggata atatgccgtg atgcacccaa aggacaagtg aaaaacaccg
     4141 taggagcggg agattcgctt acagcgggct ttattgctgg atatatgcat tcggccgatt
     4201 tcgattatgc attaagatac ggacttgcag cgggaagtgc aacggcattt tccgagacac
     4261 tggcaacaaa agaaaaaata gagcaggtac ttaacggttt tgaataatat ttcatatata
     4321 tcactttttc gctttttagt atatgtccgg tttacattgc tataattaca attgtacaag
     4381 gttatttaat ggtataatct taacgcagac atttattatg caatgaaaac ggataaacat
     4441 ggcaaataag aagaaaagca ctaagaatac aaaaaaggat aaaaagaata tagcggtatt
     4501 ggtagcaatc accgtaatcc tgtgcatagc catatctttt atttacgcat acatgaaggg
     4561 ctggtttgac ggagaaatta cccctacccc ctcactgata cggacggaag aacctacgat
     4621 agttgcatta ccttctccta gcgggaccgt taccgatacg ccctctccga caccgaccgg
     4681 aacgcccgta attgaacagc cgttaccgga gaatcatata aaaatagaaa agtttgagga
     4741 tcttttagga tatttcgaat accttctttc cgtaaagacc ggtgtttatg cattatcggt
     4801 tcaggatatt accgcagata aatcctttga tatttccgca agccggaaat ttgataatgt
     4861 atgtgcatat aaactggcgg tcgtaatgta tgcatatgag ccggaagtgc ggcaggcaat
     4921 gcctttggat acatcggttg aatatactga ggctgaccgg gacggcaatt cctccgttgt
     4981 agcaaagggc aactattccg atacatacac tatacagaaa ttatgcgagt atgtgttaaa
     5041 gagtgatgac agagccgctc ttaacatgct tttaagagtt tacggtaaaa acaacattga
     5101 aaaatatatg tccgacagag gtatgtccgg ttccgagtct tactattcac ctgacgatat
     5161 gacggtcggt tttaaattgt ttaaagataa aattgactca catgttgacg gttattccgc
     5221 aatacgggaa tatttatctt ccgaaatact tacgggcgtc tcctcggttt ccgcttatac
     5281 gaaggacagc gatattttca gtatgaatta taccgccatg gtgtatgcaa aaaatatatt
     5341 cattgtttca atgagcgcaa aagatgcaaa cttgtccgaa gcggaaagta cctttaagga
     5401 tatatgcggg ataataaata cgctttttgc ggaaaatgcg gttattgtgg aaaaaagcaa
     5461 atacagccat gcccttattt tggacaatat cgatgaacat gcaagacaaa ccgctgcaaa
     5521 tttacagtcc gttgtcatgt atcctgacgg taccgcgtca aatgtgtaca tatccgaaga
     5581 tgatatatct ttcccgtatt cggagggata ttccgacata ggtataacca catacagagg
     5641 taataactac agggactcgg gattcttcgg tagtataagc ataaaagagc ggaaacttga
     5701 agaaatatgg agttatgaga taggcggaat gaccggtgcc ggatcaatat ggccgggagt
     5761 tggttggacg ggtcagccgt gcatagttaa atgggataga gaaatgctga gcattatgaa
     5821 tgtctatgac aagttcaaga gtaggaatga actaattgaa gtcatatatg caacgctgga
     5881 cggtaatata tattttcttg acttgcatac cggagaaccc accagaaact atatctacat
     5941 aggtgtaccc gtaaaaggca cattgtccgt agaccccaga ggatatcccc ttttatatgt
     6001 cggacaaggg ctggaggcgg caaaaaccga tgaaggggaa gacagataca gaccattcgg
     6061 ttacaggata ttcagtctga tagacaacaa gcttcttcat gagattaaag gatatgatac
     6121 atttgctccg agacccggtt ggggagcatt tgattcttcg ggtatattgg atatcaaaaa
     6181 cgacacatat tatcagtgcg gagaaaacgg tattatttat atagttaagt taaataccgt
     6241 atttgataaa ggtgcaaaaa caatttttat aaaccccgag acaataaagt acaagtatgt
     6301 atccgagtcg gcatactcgt acggaataga aagctctccg tccatatatg aaaaatacat
     6361 gtactttacc gataatgccg gtatgctgat atgcctggat ctggaaagtc ttaagatatt
     6421 atgggcatac aacacgggag acgataccga cgcatcggtt gtgctggatg ttaccgaaga
     6481 aggtgtattt ttatacaccg caaacgaagt ggatataaga gggcgggacg ataacagcaa
     6541 tatccgcaag ttcaatgccc ttaccggtga gttgctgtgg caggtggatg taccggtatt
     6601 ttatgtggat gttataaacg gcggagcact tgcttcaccc gttataggca aaggtccgat
     6661 agaaaatctg gttattttca acatatgtaa gacgctggaa gattccatat gggcgggaag
     6721 gcttatagcc atagacaaat taaccggaca gatagtctgg atatacaaat ccccttacag
     6781 ctggagttcg ccggtagccg tgtatacaga ggataacaaa gcgtacattg tatactgtga
     6841 acacaacggt aagatatcgc tgcttgatgc attgacggga aaacagattg acctggtaac
     6901 attgagttta ggcagacagt acataacaat agaagctact cccgctatat atgacgatat
     6961 actggtagta ggaacatatg cggaaaagat ttacggtgtg aggataaagt aaaatgaaaa
     7021 ggataaaaag taatacaacg gttgcggtag ttataagcgg ggtagccctt gttttggcaa
     7081 tagcaaatat tattctttgc tatgttcgta atctggagaa atccgtgtct atgataattc
     7141 tgatatgcat tgcattggtg tttgcatata atctggcata tcatatttca gcaaagaaaa
     7201 aggccaaaag agaagccgaa agagaagagg atatcgataa ataagcagaa gcggtttttg
     7261 gaaatgcgaa cgtaaaagcg aacgaaaata tttgcgttcg ttttgtagca gaggatacaa
     7321 ggttgttctt ccggttagtg aagaaaaaaa caatctgacg gaagatgaga aacgagtgta
     7381 ctcacttttg aaaagaagac gcctatccag ctcggagatt gtaaaagcga caggtttcgg
     7441 gaaaaccaaa gcagtttcca ttttgaataa tcttgtagcc ggaggatata ttgaaacaac
     7501 ggggaaagga agaggaatta aatattttgc atgatgcagt atattcttta cacctttttc
     7561 ttcaaaattt aaaagtatca ccttaaacat gtgttgtact tgtgatatta tatatatgca
     7621 aataaagtat tgttttaaga ggcgcatatg ttcaatgata tgaaaattgt tgactgccac
     7681 atacactatt catccaacat acccgttgaa gagatgataa gaatactgga ctattgcgat
     7741 accgacatag ccaatattgt tgttgtaccc gacagagcga aattatcagc ggtacaggat
     7801 gcccttattc tcaaacatag attatccgac aggatatatg tgtttgcaag tctggatgct
     7861 tccaactatt tcatcaaaaa gaaaactttg ggaaaagcaa tggcaaaata cgcattgcgt
     7921 atgagagaag caggatgtga cggtataaag atgatagagg gcaaacctca gataagaaag
     7981 atgctcaatg tgcccccctt tgatgagtcg gtgtgggagc cgtattttgc gtttatggaa
     8041 gacaccgctt ttcctatctt atggcatgta aacgatcctg aggagttctg ggataaagat
     8101 aaaataccca aatgggctat gcagcaggac tggctttatg atgaaacata tgttaacaac
     8161 gagaaacaat atgaggaagt gttgaatgta ttaaaaaagc atcctgatct gaagataata
     8221 tttgcacact tctttttcct gtcaaaacaa ttggacagat tatcggaaat attggataag
     8281 tatcaaaatg tatatgttga tcttaccccg ggaatagaga tgtatattaa tttctccgaa
     8341 aataaagaaa aagcaacagc cttttttgaa aaatatcagg acaggattat gtacggaacc
     8401 gatataggta caaaaggtgt ggtggacggt aaaagcgctt ctttagaaga ggaaaagaaa
     8461 cgggcggatc tggttaaaag gtatgtttct tccaaagcgg attttattat cgacaagcat
     8521 tattcctacc ttatgcacga taacgatttc acattaagcc ctctgggctt aagtaaaaag
     8581 atacagaaaa agatattctc tttaaacttt ctggatttca tgggccatac gccttatccg
     8641 gtcgactcaa ataaggtgtt gcgggaaatc agaaaaaccg tagttaaact ttgtttaatg
     8701 aaaatcggag ataaatccgg tactgaatac gattttgcat cactgaagaa agcttataaa
     8761 caattggaaa agatttagga ggatattatg caaaaaatca aaagaaacta cctgccttta
     8821 tatgctctgt ccggtttcgg acccgggctt ataaacctaa tgatttcagc ctatcttata
     8881 gacggcttga gtacggcagg ttttttgaaa aacattgaaa actggacata tgccaataaa
     8941 accattgtta tgaccgcttt gttcagtgtg tttgtattga tagcgaaaat cgttgacggt
     9001 gtagcggatg taccgctggc atcactgacc gatacgctga agaccagatg gggtaaaagg
     9061 cgtccggcta tacttatagg gtacataccc atgattatca catacatatt attctgtttt
     9121 cccttggaaa tagcggagca cagtttgtta aacacaatat ggttcggtgt gttgcttatc
     9181 tttttcttta cttcctacac tatgacactg gtaacatatt acggtactta ttcggaagtt
     9241 accgagaatg acagcgatcg tcattatctt tcttcctgga aagcgttttt tgataccgtt
     9301 caatattcca tcggttatgc cgttatacct ttaatggtag gcgggttgaa tatccggcat
     9361 attgccctta tactgtcacc cttgcttctt accatgataa tacctttttt cctactgaaa
     9421 gaacaatcga cgctaccgga ggatgttgca aagcgtgtag aagcgggaga agcggtggaa
     9481 gaagaagtac ctttcatgga gagcataaaa ctgacaatta tgaacaaggg atttataatg
     9541 tggttagtgg ttttcggaat ctttttcttc gggcttcaga tgttcttagc gggacagaat
     9601 gtactggcat cgggacctat gggacttaac agctggcaga ttgccattat aaattccgca
     9661 gcattcgccc ctgttccttt aatgctgtat ttctacagga aagtaatgaa aaagaaagga
     9721 ttccgatttg cattccagac ggctatgggt tcttttgccg ttgccatgct tatgttttcc
     9781 atcgcatata tcaagtggat acccgatgag tatataaggt tggcaatagg agcaacggga
     9841 gctacaatag gaagttacgg tttaggtgcg tttttctccg ctccttatgc gatacccgca
     9901 caggcggctg ccgatgaatt gaaggctacg ggtaagagcc atccgtcaat gtattttgca
     9961 atgcagggat tgtgtacggc tcttgttgga gcattatcca caagtgtggt gtggctcaac
    10021 atcaaagaga ttacgctacc tgacaatccc gtgttcggag ctcatttgat gccttatata
    10081 gtaatagcag cgtgtgtaac agccataata gcggccaaat acatgcctaa agaatacaat
    10141 gagatgggga aggaaaaatg agtgcctatg ataacaccgg ttcagggaga ttgtatcttg
    10201 ccatagatat aggagggagc tccattaagt acggggtttt ggatgagtcc ttttcttttg
    10261 tgcataaaag caaaataccg tcgagaatat ttttgggagg cgaggagctt ctgaaccggg
    10321 taatgggaat aatagcggat atgttgaagc aatatccgct ggagggagtg gctatttcca
    10381 cgacgggagc ggtggacagc agcaacgggc gtgtattgta tgccgacgat gtaatggtcg
    10441 ggtacaccgg taccgatttt cgggaaataa taaagcaaaa attcaatctt agtgctaccg
    10501 cctgtaatga tgtgaattgt cttgctttat cacaaattca gtttaataag gggaaggatt
    10561 tcatagttgt agccataggg accggaatag gcggcggagt ggttatttcc gacaagctgt
    10621 acgagggata cggctattcc gcgggagagt tcggacttat gcacttaaga gacggtaaaa
    10681 actgggaatc gttatgttcc atcagtgctt tggtcaagac ggcaaaagaa cataaacttt
    10741 tggtatcaaa cggaagagaa ctgtttgatt tgtttgatgc cgacaacgac accgcttacc
    10801 ggataataga tgcattttac aaggatatgg catcggctct ggctaacctt atatatgctt
    10861 tctctccgca gaagattata atcggcgggg ggattagtgc aaggggagac agtcttgtag
    10921 cgggctttaa gagatactta agtatgatga cggatcaatt ctattatgaa aagaccgcca
    10981 ttgaatgcgc ggtatatgaa aatgatgcct caatgatagg ggctttacag cattatttca
    11041 ttataaacaa cgaatagttt tacacataag ttgtagaagg ttgtttttaa aactttacat
    11101 gtccgtaagc atttgaaaat caatcctcaa atttagttac cggctctatg gtaaaactgt
    11161 gctcgaacgg agtaagatag ttaaagaata tatggtattt atcggtatcc agaggttccg
    11221 aaccgaacaa tccttgggtg atgtatgtat cgtttcttac aggtcctatt ttcactccgc
    11281 aagttccgaa ttccgctctg acagcacccg actcgggcag cagtattcct ccggcatttg
    11341 cttttattgt atatccgtca cccatgataa aagcattgtt attaatagca atatccaatc
    11401 ttacggatac cttttctatg ccccgggctt tgagacttat tctgaaccct ttttccgtca
    11461 aggtaactaa aatgacggca tccaccgtaa gcaactcgga ggttgctctt ttggacttgt
    11521 ccatttccca gaaatccgcg gttccctgat attgcccgaa cggatgataa tatatgcctt
    11581 gaccgtgata gtccattgca aaaccgtttt ctatgggacg gatattgtta actttaatat
    11641 gtcgctcgtt aaaaaagagt attcctccct gaataaacat gtcgaaactc ttatatttaa
    11701 gatacaaaaa ggtttctctg tcttcaataa ccgataaagt cataccgttg tgatgaatcc
    11761 ttaatatctt ggattttttg aaatgcttta catacggctg aatttcaaac ggttttatat
    11821 gttccggtat tatcaatacc tccggttccg ccatcatggt ggccaatcct atagggtatc
    11881 ctcttccttt ggagaaaagt tcattctgta tggcttttgc cacaccgagc agttgttcat
    11941 ccttaagcag ataacccgct ttaagatact ggtagagata tttgtccaga taaatgtttt
    12001 cttttccctt atcctggcgg gtggaattct gcgtgaatat gagaaagtcg gtatgtatgt
    12061 agtaggtcat catcataaga ttcctgcgca cgaattccag cagttccggt ttatcaagtt
    12121 cctgtgctat tattaaaagt gcttcattat tgacaaaatt atatccaccg gaagaccgct
    12181 ccgaatattc gccttcctca tcgcaatcaa tactttcggc cagatattca tttattctct
    12241 taagatattt tttatctccg gtcatgttca tgaccatagc cagagctgac gttataaccc
    12301 atctgtggtt gggggtatgg aatcctcctc caaaaactcc gtccgctatt tttactatga
    12361 aaccgtaaag tcttttctta aagagttctc ccgtttcatc ggttttttta tctttaatga
    12421 tattgtaaag atcgaccaga ggctgtattg agaaggatgt atccggtgcc gaccggaaat
    12481 tgcacatata gaaatccata agtccttcgg gacttaagtt tctttcaaag aactccagaa
    12541 cggagaaggc gaactccatt gctttttttg atttatagta tttgctgtcc tcattgaaat
    12601 acagtaatcc gggagcatat aaaacccctg cgatatgagt gggtacaaag ccctcatcca
    12661 caataatgac accgtctttt ctccagccgg acgcctgtgt catctgattt cttataagtc
    12721 tttccagata aatttcatat gattttaaac ttgctttgta cagatccaat gtatatttca
    12781 ttacatctct ccttgccctt ttactcatta tagccgatgg gcaattttaa gcaacatcaa
    12841 atcataagtt atcgggattt tgggtaagaa actgttttag aaaaacagga aaaagagata
    12901 tcagctttta taaacttcac tgcgatgtcc gatgttcaac gcgaggataa taaatttttt
    12961 atcctctata tgacaaatca aacgataatc gccgattcgg catctccata gaccttttct
    13021 gttttcaatc agagtttttt tgcatgttcc gagattttcg cagtctaaaa ggtttttttc
    13081 aatccaggca ttaatcatca attttgtata gcggtcgagt tttttaaatt ctctttcaaa
    13141 tcttgatgtc acttcaatcg aatatgtcat gtctgaagct ctttccaaaa ctctttaata
    13201 gggcggcttt gttttccgct gtcctcatat tctttgtacg cagcatcata aatcgcaata
    13261 tcatattggt cttcaatttt ctcaaacaaa gctcttttga aagcctccgc caatgtgtac
    13321 gaatgtagtt ttgcatagct ttccgccaat gctctttctt cttcagttaa tctgattgaa
    13381 aaggccatgt tatgtcacct ccatctccat agtacattgt actgcaaaaa taaagaattt
    13441 aaagcatttt tgcagtctgt tttaaagttt cagttactaa tatgcttatt acaatagaaa
    13501 aactgtgtat tcctgcgaac acacagtctc tcttcggaaa gaacaattct atcggttttt
    13561 taagaccgct ctaatcttcc actgcagctt tctttgtatt aatcgcccgt ataacagcag
    13621 ggtccatctt gggttttctg tcatatgtgt aaagtccgtt ctgttcctgc tcgacatcgg
    13681 tcagttgcgt gtaacagaat gccccgatat tgggattatt taacagcact tcggtcaatg
    13741 ctccgtatcg ctcgataacc tcatcaaggc tcttgggacg ttccccgtat ccccagccgg
    13801 tcatatcggt ttccgaccac catattccgc cgtattccga tatgaatacg ggagcgcaat
    13861 tatcgtcaac atagccgtca taattcttac gttgcttgtc cagctcggca tatcgctctt
    13921 tgaatgtaac cggattctgg tcatagtcgt gcttatccct tatgtcggat gctccgctta
    13981 catgtgtcca tcccgaagtg tcgataaccg gtctggtagg gtctgttgct ctggtcatgt
    14041 cagcgacgaa tttaataaga tcggggttct gatctttctg tgtttcgttg aagggacacc
    14101 atccgataat tgaaggatga ttgaagtctc tgtccattat ctccagccac tcgggaacaa
    14161 agcgctccca tgccttgtct ttggatacat ccaatcccca gttggcatgc tcttcccaca
    14221 ccagatatcc cagaacatca cagtgatata aaaatctcgg ttcaaatact ttttcatgca
    14281 gccttgcacc gttgaatccc atagcctgtg accttattat gtcatttttc agttcttcat
    14341 cggtgggtgc ggtgtatatg ccgtcgggat agaaaccctg gtccagtatc agtctctgaa
    14401 atacaacctt accgttcaga taaaacttgt tatcatgata atatatactt ctcattccga
    14461 agtaactgtc cactttatct tcatccagtt ccagtttgag ggtatataaa ttgggggtct
    14521 cacagcacca aagatgaagc tctttcagtt tcatattgaa ctttgcatgc tttccttcta
    14581 ttatcgcagt gcaggaagcc atctttttat tgtcaaaata tgcggtagcc tttattttct
    14641 taccgttagc atttctgcac aatacatcaa aatatatact ggatgtgttt atgtccggta
    14701 tacatttgaa agacgctata ctggaagacg gaaggttttc cagccatact gtctgccata
    14761 ttccggtagt tcttgtatag tggcagcctc tggaaaaata ccctacggat tgctttcctg
    14821 caggttgttc catggttctt aaaagatcgg tagcacttat tgttatggtg ttttcacctt
    14881 ctttaagttg ctctgttatg tcaaaactga atgaagaata tccgcccata tgagttccga
    14941 ccgattgggc attaatccat acttcggtaa gataatcgca tgcgtctatg tgaagcaagg
    15001 ttcttctgcc gttctccagc caactcttat ttatcctgac cgtccgtttg taccacacgc
    15061 actccataaa gtctttgtta tttactccgg agagtgcact ttcggggcag aagggaacaa
    15121 ttatggagga cgagagtttt gcatctttat acaattcccg ctcttttccg cttcttccgt
    15181 gatcaatttc aaactgccat ttaccgttta gattgatcca ggatttcctg tacatctgcg
    15241 gtctcggatg ttctttacga ggtatttcag ccatattgtt ggtctccttt gaacagtgat
    15301 gtatatagtt tataacctgc atatatgtct ttcaatgcac aatacttcta tcaattgact
    15361 tatcctactg ttctgatatc atattcttga acgggagtta ataatgaaaa tacataatgt
    15421 acggcatgaa tggccggaaa aggcgggttt tatactgaac agaccgcacg gacccaaaga
    15481 atatatattt ttacattact ttaatccggt tgacttatta tgcaataacg ggataatacg
    15541 cacaaatcct catgcatgta ttattgtctc gcccgataaa gcacagtggt tcagaagcga
    15601 gcagaaactt gtacatgact ggtttcattt tacaggtgat gtggaaagta atatgcaaaa
    15661 gtacaatctt acgcccgata ccgtatatta tccgtatgac agcctggaaa ttaccggaac
    15721 ggtacgcaag atggaatcgg agttttttac cgatatgcaa tactccggga aactgtgcga
    15781 actgtacata gagcagattt ttatcatgat ggcccggtcg gaaaaagact caataaatgc
    15841 agcaaatgtc aaaaagcaga tatcggataa agtggaacag gtcagaagaa aaatgttcac
    15901 ctctccggcc gaagactggc cggtttccaa aatggcaaag cttgccggat taagcgaatc
    15961 acggttctat acggtataca aatcggtata taagattacc cctaacagag acctcattat
    16021 cgcacgtatc gaaaaagcca ggactctttt gcagactaag gattattccg taaaccaagt
    16081 tgccgagatg ttaggttaca gcagtgttta tcactttatc cggcagttca aatcggtgac
    16141 gggagttact ccgggaagtg taagaaaatg ataaaaaagc aaaaattaat ggattttttg
    16201 tgttaaatat ataacatttt taaaaaaagt gtttacaata cgaagtatca attatatact
    16261 gttttataaa cttggtagtt tttttattgg aaggtatgac gtgaaatatt attcaagagc
    16321 attaatcatt gtcattatat tagctacaat atttgtaata ggaatggcga tagaatttga
    16381 ccattccaat ataattatta agagtgattc tctcaagccc tttcaggaaa tgagagtatc
    16441 gaacggaatc aatccgtgtg aatacaaagc aaaagtaata cttaataatg atttcgtcat
    16501 gccgcagact ctgttgttca agaccagtca tttgcgtttt aaaatatata ttaacgaaaa
    16561 aatggtctat tctttcggat atgaagaaaa tgccgtttct tttctgaaat ctcccggaac
    16621 ttcctatcac ctggtggaac ttccggctat gagcagcaat aaagagattg ttttcgattt
    16681 ccagagtcct tactccaatt accacggttt cctgcctgat atacagtacg gtacaaaagg
    16741 tgaatgtata gcatactacc agaataaatc catgtattcg ttgttcagtc tgaccgttgt
    16801 gctgttgggc ggattgacta taatgatttt gtatttcgtt acaaagaaca agaagtatgc
    16861 actgtcaaaa tcctttctat atataagcag tttcatattc ctggtatcga tttggttgtt
    16921 tttccaaagc gattcctgcc agcttatgtt cggatatccg cagataatgt attttgccga
    16981 cctcataagt ctggtgtact gtttcccgtt cctttgaatt tatacatata tgatgtttcc
    17041 aaaaatgaaa aaagaaagta tatgttatac tttgcttatg cctatgccgc aacattggtt
    17101 ttaaacttac tgcttcaggt attcggtctt gtggatatgt tccagctggt gcttcttact
    17161 cattcgctta tggctttgaa tatgattgtc gcggtatatg tcgtacatgt agagttgcgt
    17221 attgagaaaa acaaggatct tcgcatgttc attgtacctc tggcgctggt atctgcaggc
    17281 ggtcttcttg agatgattct ttactattac tccaatatga cacagacatc cttcgcgtta
    17341 cgtattgcgg ttgttgcatt tttggttgtg ataattatta acgccataaa gagttattac
    17401 gataatctgt tggaattgaa acaggcggaa tattatgaac agctggcaag tgtggacatg
    17461 ttgacaaatc tgggtaacag aaataaatac gaacagtttc tgcagtctca tgaacctaat
    17521 caacctttga ctgcagtact ttttgatttg aactatctga agtatataaa tgataacttc
    17581 ggacatgtgg tcggggacag tgcattgaaa aaatgtgcgg aatgcataaa agaagctttt
    17641 tccaataaag gcgagtgttt cagaatagga ggagatgagt ttgccgttct gacaaaagcc
    17701 gatgataatc ttcaaaaaga atgtaatata ttcgagaaac tggtgcaagg atggaacaaa
    17761 aggctttcat atatattctc cgtcgcttac ggagttgcaa gattcaaccc taaaaccgat
    17821 caatcggcgt atgataccgc aaagcgtgcg gattttgcta tgtatcatat gaaagaagaa
    17881 cagaaaagga aaataatact gtaactgtat tacattacat accgtaaagc gggagagagt
    17941 tatctggact ctcctttttt gtattgacag taataggaaa atgcatatac ttatgtatga
    18001 gcaactgttc atatgaaagg tgagtgtaat gaaagaacaa attgatatct gtgaatacat
    18061 aaacatgact gaggaagaag taaaggaagt cttaaagagt attccgagtg aagataaact
    18121 gtataacctt tccgatcttt tcaaggtgtt cggtgacaag acaaggataa agattctatt
    18181 cgcattgtcg gtcgccgaaa tgtgtgtatg cgatatagca agtctcactc ggatgacaca
    18241 atcggctatt tcgcatcaat tgaagacatt gagagcaagc aaactggtta aatacagaaa
    18301 agaagggaag gccactttct attcgcttgc agatgaccat gtcagaacaa tattacaaca
    18361 gggaaaggaa catatagagg aataaactat gaagagaaca tacaaattaa acaatcttga
    18421 ctgtgcggca tgcgcagata aaatagagag aaaaatatcc aaattaaccg gcgtaaagca
    18481 ggtaaactta aatttcttta ctaaaaaagc cgcaatagaa acggatagcg aagagatttt
    18541 ttgcgagtgt gagaaaatat taaaaaaata tgcgggtgct tttgagatag aatgaaaaaa
    18601 cttaataaaa agcagaaaac aacgttgata aggatgggta tttcggttgc tcttctagca
    18661 ccggcattac taatacaaaa ccgggtaata tctcttatac tatacatact ttcctatctg
    18721 gtgataggtt atgaaatact cataaaatcc gtaaaaaata taataagggg agatattttt
    18781 gacgagaatt tcctgatgac tattgccaca ataggagcac ttgtaataaa acaatacccc
    18841 gaagcggtag cagttatgct tttctatgag ataggagaat ttttccagga atatgctgtg
    18901 gaaaagtcca gaaaatccat atccgatctg atggaaatac gtccggagca tgcaaatctt
    18961 atcacaaacg aaggtatcat aacggtgcag cccgagacag tgaaagcagg ggatataata
    19021 gtggtcaagc cgggtgaaag agtgccgctg gacggtattg tcattgaagg gcactcggct
    19081 ctggatatgt cttctctcac cggcgagtcg ctgcctgcgg atataaaccc ggatgatacc
    19141 gttttaagcg gaagtattaa tacattcggt atgcttaaac ttcaggtgga aaaaccgtac
    19201 aatcaatcga cagtagcagg cattttggac cttgtggaga atgcttcatc aagaaaagcc
    19261 aagaccgaaa ggataatcac gcgattttcc cgtttctata caccggttgt agttatatgt
    19321 gcagttctgc ttgcaattat tccgccgctt ctttttagac aacggttaac cgattggata
    19381 tacagagcac tggtgttttt ggtggtatcc tgtccctgtg ctttggtttt atcggtgcct
    19441 ttgagtttct tttccgcatt ggggtcagcc tcaaaaaacg gtattctggt aaaaggcgga
    19501 aactgcttgg aagcgctggc aagcacaaac tccgttatat tcgacaagac cggtactctt
    19561 acaaaaggta tattcaaagt gacggcaatt catccggaga aactcaattc acaacaactt
    19621 ttagatattg ccgctacggc ggaaagttac tccgaccatc ccatctccaa gtcgcttaag
    19681 gcggaatacg cgaaacaaat agaaaacggt cgtataagtg agataaagga aatagcgggg
    19741 cagggtataa aagcggtaat tgacggcagg aaagtaagcg taggcaatga aaatcttatg
    19801 gaagcggacg gtgttgaatg gcacaggtgc aatcttgcgg gaactatagt gcatgtggct
    19861 gttgacgacg agtatatggg gcatatagtt atatccgacg agattaagaa ggatgcacct
    19921 caaaccgtaa acctgcttaa agagctgggg attaccgata tatccatgct gacgggagac
    19981 aataagtcgg ccgcatacca tatagcggat aaggcgggaa tagagaatgt ggctcactcg
    20041 cttcttcctc atcagaaggc ggaacatgtt aataacaagc ctaaagataa aggagcagtg
    20101 gtctttgtag gagacggcat taatgacgca ccggtgctgg cccaagccga cataggcata
    20161 tccatggggt tatccggctc tcaggctgca atagaagctt ccgatgtggt tataatggac
    20221 gacaagcctt ccaaagtacc gctgctgata cgtctttcaa aaaaaacaat gagaatagca
    20281 aaacagaata tggtttttgc aatatcggtc aaactcattg tacttatagc gggagcagcg
    20341 ggattggcct cattgtggat agcggtattt gccgatgtgg gcgtgtcgtt tcttgccgtg
    20401 ctcaatgcca tgaggttgct taagaagaag gtgtagcaac ggtataaagg cgattgaaca
    20461 ttttaaatat atgacaaaat attcgatatg ttagcatgga ctttctgtgt gtagtcttta
    20521 gattttaatt gacggaagag aagaagggta tgaaaacttg aggtcacatt ttgtgacctc
    20581 aagtgaggat ggcaaacatg gttttgaggt gccaaaatgt cacttcaaaa tctgaatccg
    20641 ggttaagtga tccgctccaa aaaaggtaac actttaatct cactgcaccc ctaaagggta
    20701 acacttttta tctgtcttaa cgataaagac aattggttat tttcaatata tgacaaaata
    20761 tgcgatatgt tagcatggac ttgcgctatg cggcaaagta aaattcaaat agataggtaa
    20821 gcagaaatac agctttatgg ttttaatatc tattcataca attttataaa tttgcaggac
    20881 caatgtatat tgtaaaatta cgggaaaaat aataaaaaag ttgtcgaggt ggcagagaat
    20941 ggataaaaat atttggcagc cgtatctgca aacaagagca gaatatcttc cggaagacaa
    21001 atcggtaacc atggctaccg atttgaaaca gatgaatgat actgagttgt ctgttttaca
    21061 aaaaagattg gtaaatttcg aagattttcc gatagagaag catttattga gattggtttt
    21121 tcgcatcata tggaaattct acgtaacaca aaagagttaa atgaacgact cttctatatc
    21181 cgtgaatgtg catcgggata ttggagcagg cgattgctga gaacaaggct gaaagaagat
    21241 ttatacaatc gcaaaggcgc attggttaat aatttttcgg aaacattgcc ggacagttat
    21301 aaagatttta cacccattat gtaagaggta ccgaagatgt tagatgaagt agaccttgat
    21361 gaaagtgtaa aacggaaata aaaatacgag gtaggaatgc aagtcgaaaa ccttataaat
    21421 ttaccgaaag tattgttttg ctaaggaggc aaacatggca gatagagatt tggctgaggt
    21481 ttatcatgtt gaaacgaagg ttcttaatca agccgttaag aggaatatag atcgttttcc
    21541 tatagaattt agatttcaat tagatgatga agaaaaaaca gaactggtca caaattgtga
    21601 ccggttcgaa acactaaaac attctactag aagtccttat gtttttacag agcaaggaat
    21661 agccatgcta tcttatgata tatactttta gaggtgtata ttatttgatg aaataaatag
    21721 cgatatcaaa catgaaaaaa catgaagtgg tcgcaattag cgaccggtta tggagaatcc
    21781 ggaggtaact atggcagacg acaaattacc tgtgatagta gaaaacagag aaattcagga
    21841 catgatatat gccattagag gtaagcaagt catggttgac agtgaccttg ctgatttata
    21901 tcaggtagca accaagagat tgaatgaaca agtcaataga aataaaggta gatttcctcc
    21961 gcagtttatg ttccagttga ccgaagatga atatgagaac ttgaagtcgc aaattgcgac
    22021 ctcaagtgat gggagtaagc atggtggacg aagatatatg ccgtatgttt ttacagagca
    22081 aggaatagcc atgctatctg cggttttgag aagtgatgtg gctgttcaag taagtataaa
    22141 aatcatgaat gcttttgtgg aaatgcgccg ttttttagtt agtaatgccg ggatttttga
    22201 gagactaaat cgtgtcgaat taaagcaact tgaaacggat caaaaactcg aaaaagtctt
    22261 caactacatt gctacacaaa cagaagtgaa gcagaatatt ttttatgacg gacaaatcta
    22321 cgatgccttt agctttattg tggacctgat aaaaaaagct cgaaataagc taatattgat
    22381 agataattac gtggatacaa atactttaaa tctgctatgt aagaaaaatc aaggggtttc
    22441 tgtttttatt gcaggctcgg gaaatggaag tttaacaagt aaggatatct ccaaatttaa
    22501 cgctcagtat caaggcttaa cagtaaaaac gagaaaggat tttcatgacc gatttttgat
    22561 tatagatgat ttggaagtat atcatatagg tgcttcaatc aaagatgccg gcaaaaagag
    22621 tttcggaatt tcaaaaattg aggatgaagt attgaaagat aacttaataa aaaaagtgaa
    22681 atagtgagga tgtcgtggaa gaagacatgt aatatcttgc actaacttaa ttcgaggttc
    22741 cggtgcgaag aaattacgtt ttacaagatt atgaggagca ttgttcttta tctctattcg
    22801 cctatgtcaa atttccttct gtagatatgt gtcttggtta agtacttata aacttatcga
    22861 aagtattgtt ttactaagga gacaaacatg gctgatgaca agtcccctgt gcttatagat
    22921 gatagaaaaa ttcaggacat gatatagcat tagaggtaag taagtcatgg ttgatagtga
    22981 tttagcagaa ctgtatcaag taccaagggg gaacttgaat aaagcgatga tgcgaaatgt
    23041 tgatcggttt cctgagagat atttttttca accaataaat gaataatatg accacttgag
    23101 gttccaaaat ggaatctcaa gttcgaagct taattatggt ggcagaaggt taaagtgtta
    23161 cctttttggg gagcatatta tgtttccgag agataacaat ttggcagtca caactgccag
    23221 gttggttcag aaattcagga acaaatactg aagcaagttg cttcagaaat gatttttctt
    23281 catttttgca tatgttcctg atgcaccatg tgcttattga caagggatat tctgttgcct
    23341 tacaatttaa gcaacttctc ccccaggcct ctatcacaat ctcaggattg gacacgcaca
    23401 cttgggggcg ttctgtttat tggaagtttt gtgaccccgg aaggatttta aatcagtata
    23461 taaacaactt gagattctga aatcttgttg tttaagcata tcgttagagt tgataatagc
    23521 cccgtgagaa gatataatca agcagtgtgt ggtattttta agtttgtggg gtatgctgaa
    23581 tgaataatat gactttgaaa aatgtccaac gttttgacat ggtaaaagag atacgtgtca
    23641 taaggtggta tctgagactt ttaacctgga taatcagttt tcccgcagta tggtttcagg
    23701 ggacgaaaat aaggaagcag ggagtgaaag gtataaaagg tgcgtatctg atgctgtgca
    23761 atcacaacgc attttttgat ttcatggtgg caaccgcggc catatttccc agaagagcca
    23821 actatgtggt ggctattgac gggtatataa agcgcgaatg gctgttacgc agtgtgggct
    23881 gcatatgcaa aagaaaattt accaacgacc ctatacttat aaggcacctt ataagaatag
    23941 ccaaaaaagg cgagattata gccctatacc ccgaagcacg atattcatta tgcggtacca
    24001 atgccgtttt gccgcagtcg cttgcaaagc ttgtcaagca tctcaatatt cccgttgtga
    24061 cactcattac aaaaggacac cacataaact ctccgttctg gaatctcgaa aagaggggaa
    24121 accgtaccga agccgacctc aaactcataa taagcaaaga tgaaatcggt aaaatgagtg
    24181 tagaagaaat agatgaactt attcacaaag aatttatata cgatgatttt aagtggcagt
    24241 atgacaataa ggtaagagtg aaatacaaaa aaagagccga aggtcttcat aaagtattgt
    24301 atcaatgccc caactgcaac accgaatata tgatgggctc ggaaggtgcc gaaatattct
    24361 gtaaacaatg taataagcga tggtatatga ccgaatacgg gcgcctggaa gcaaaagaag
    24421 gaaataccga atttgcccat atacccgact ggtacgaatg ggaaagagcg caggtaagaa
    24481 aacagattga tgacggaaca tattatttcg acaaacaggt tcgtatagat tccctgccca
    24541 acgcaagagg atatattgat ttaggtaaag gacgtctcat tcataacatg gaaggattta
    24601 ccctgaccgg aatgtccgac ggggaagagt ttatgattga atggcccgcc aacagacttt
    24661 attcctgtca tatagaatac gactatttag gtaaatacgg tgactgtgtg gatttaaata
    24721 ccatgaatga cactttatac atctaccctg aaggtgagga cttcgcagta accaagttat
    24781 ccctggctac ggaggaactg tataaaaagc tgaatgatat ggactaaagc agataagctg
    24841 tcacctaagc catgatttgc accttaagtt tgaaatatag tcagaataaa agaaacggaa
    24901 gagcgtacaa aataatacaa aatgcttgaa ttgtactcat acatatagta aaatgcaggc
    24961 aaggagtaaa acaatggaaa tcaagttaga aagtttaatt tcttacgaga aatttaagaa
    25021 tgatatagac aatgtgctgg aaatggttga gaaaaacggt caagctgtca tattgaaaga
    25081 taacgaaccc ttatacataa tactgaagag cgacagatat tcaatcctaa aagagaggac
    25141 tttatcaaag aaagtaaata agatgacttt gcaggaagca atgaagattg tgcttaagga
    25201 agtgaagggt agaaagttgc acgctgctga attgtctgat gagatatata gaagagggtt
    25261 atattttaag aaagacggaa cccaggcaag atacaatcaa atacgttcaa gatgcgggca
    25321 ttatccggat atgtttgaag ctttacccgg aaatattatt tatttaaaag aaggtgtgga
    25381 gtaatggtaa atatgaggat agaatacata aggcaaatta ttaaaaaggg cgaaagcatt
    25441 gatatagaat tcaaggaaag taaaagtaat atcagtaaag atgtttacga gtcggtatgt
    25501 gcattcttaa atcgaaatgg cggacatata ttccttggag ttaaagataa cggtgatatt
    25561 gtgggtgttg ataaagtggc gataggcagg attaaaaaag actttgtagt gacattaaat
    25621 aatcctcaaa ctttgaatcc tactttttac cttgcgatag aggaaattga aattgacgga
    25681 aagacaatat tgtatattaa tgttcctgag agttcacaag ttcaccggtg taaaggaagg
    25741 atatttgatc gtaacgaaga tggggatttt gatattactg ataatacaaa tcttgtttcc
    25801 ggcttgtata ggagaaagca aagtacttat gatgaaaaca gaattttccc gtatgccgga
    25861 atggaagatt tggatttaga gttatttgaa aaggttagaa ggcttgtaag gagtctggat
    25921 tctaatcatc catggatttc aatgagtaat gaagaaatac taaggagcgc aggactgtat
    25981 cttatggatc ctgctaccgg taataaagga attacgcttg gcggtattct gcttttagga
    26041 aaagaagaac tgatacacgc agcacttcct catcataaaa cggatgcgat agtgagaaaa
    26101 gttaatttag accgttatga tgaccgtgac gacataagag ttaatctttt aaaaagttat
    26161 gaaagactga aacagttcgt tgcaaaacat attgacgaca aattttatct tgaaggagat
    26221 cagcgcataa gtgtgagaga taaaattttc agagaggcaa ttaccaatct gttaattcat
    26281 agggattttg caaatccgtt tccggcaaag tttattatag aacgggacag ggtatacatt
    26341 gaaaacagta acagacctca cggtaatgga attattgatc cggaaaactt ttcaccattt
    26401 cctaagaatc cgacaatcgc taagtttttt aaagaaatcg gttgggtgga tgagcttggt
    26461 tcgggagtta gaaaaatttt caagtatagc aaagtctatt cagacactaa gcctcaattc
    26521 attgaaggag atatttttaa gacaattatt tatgtagatc cattaataag cattatcaat
    26581 gatagtgatg taagtggaga agaacaaaga attcaaatga taattgagta ttgtaaagta
    26641 ccgagaagca gacgggaaat tcaggattat attggaataa aggacaggaa agattttacc
    26701 aaaagattat tgaatctact tattgaaaaa gggttgttgc gaagaacttt tccggataag
    26761 ccaaccagtc caaatcaaaa gtacgttgca gaagaatata ctgaagctga ataatgctat
    26821 atcatgattt ttagtgtatt tttaggatat aactaaaagc tatgacctaa gctatgacct
    26881 aagctgtgac ccaagctacc ccccaagcta ccccccaagt taccccccaa gctacccccc
    26941 aagctacagc ctgagttatc gcaaagcaga cgggaaattc aggattatca ggattatatt
    27001 gaaataaagg gcaggaatga tgctataaca gggtttttag tgtattttta ggatataact
    27061 aaaagctgtg accaaagcta tgaccaaagc tgtgaccaaa gctatgacct aagctgatta
    27121 tatattctac cggtgaagca aacttcccca attggggtgg aatttaacac atacagaata
    27181 tgcttgattt tctcgatgtt gctgaaactg tagaaaagca gagaattagc ccaaaattat
    27241 ctcatttgca tagtatataa tactgctcat tttctattaa aaatatctat aatatcgcta
    27301 tgcatatttt tcaacattgc ggttttgtca gtgattgtag aaaaaatgta ataacacata
    27361 tttacttttg tgctaatttg agatatgata ttagcgcgaa aggaaattgt tatggacact
    27421 gagtatgttg taaaacaact tttacaagaa aatgatggct ggcttacact aaaagaagtc
    27481 ttggaacagg gtatcagcaa atatgaattt tacaagtttg ctgaaaaaag cggattggaa
    27541 agagccggac aaggcattta tgtttcggct gatgtttggc ctgattctat gtatctgatt
    27601 catctcagat cggcgcaggc tatcttttct catgaaacag ctctgttttt acacgatatg
    27661 actgaccgag aaccgtctta ttattctgta acggtaagga caggttataa tccgcatcgc
    27721 ctgaaggaag acggtatcaa ggtttatact ataagaaaag aattgcatga acttggtaaa
    27781 agtaatataa aaactccgtt tggtcatata gttccggttt atgatataga gagaacatta
    27841 tgtgatattt tacgcagcag aaatggtgta gaaatacagg tatttcaaga tgctttgaaa
    27901 tcttatgcaa aacgaagaga taagaatctt agatttttaa tgcaatatgc ggaggctttg
    27961 aatgtaagtg caatactcaa accatatttg gaggtgcttc tttaatgatt aaaacatcaa
    28021 gacaattaaa agataaaatc cgtaatcttg ctaaagaaaa atctgccgat gcgcaaattt
    28081 taatgcgaac atatatgatg gaacgacttc tggaacgtat ttctatttcc gagtataaaa
    28141 agaattttat attaaagggt ggcttgctga ttgcttcttt agtgggctta gatttccgta
    28201 gtacgatgga tgtcgacaca acaattaagg gttttgatgt taatctgacg agcgttgagc
    28261 aaatgataca aaaccttatt gatgttccgc ttgaggacgg cgttcttttt cagataaaaa
    28321 atatagattc tattatggag gaggctgaat atccgtgcat aagagtcagt atgcaagcct
    28381 tatttgatgg aactataacg cctcttaaaa tcgatatatc aacgggtgat gttataactc
    28441 ccgatgccat tcactattct tataaactga tgtttgaaga taggtatatt cagctactct
    28501 catacaatct tgaaactgtt ttagctgaga aattggaaac tatcatgaga aggcatctta
    28561 caaacacacg catgcgtgat ttttatgatg tttacatttt gaaaaagctc tatgaatcgg
    28621 aaatttcaaa accgctcctt caagatgcgc ttgatgcaac agtacaaata cgaggaagta
    28681 cggaactttt aaagcatgca gagccttctc tgaaagaaat agaaaatagc gatgatatga
    28741 gaaaattatg gctttcttac caatctcatt atgaatatgc gcagaacatt tcttgggatg
    28801 ctgttatgga agctgtaaaa gcaacatatt gacgacaaat tttatcttga aggattgtta
    28861 aaaaagatta ttccgaataa gccaaccggt ccaaatcaaa agtatgttgc agaagaatgt
    28921 gctgaggttg aataatgcta taaatgattt ttagtgtgtt tttaggataa aactaaaagc
    28981 tatgacctaa gctgtgacca aagctgatta tatattctac cggtgaagca aactttcccc
    29041 aagttggggt ggaatttaac gcagacagaa tatggttgtg aatgaggtta agttagcctt
    29101 tactccttca tttttgtgtg atatattctt tcaagtattc taatcggagg atttatgaag
    29161 aagtataacg catttgttga aaagttgttc tggctgatga ttataatcaa tccgtttctt
    29221 gatgcggcta caggtattta taagacattt tttgaaaaag aagatgcaac tttttctctt
    29281 tctcccagtc tcataatccg tttactcttc ttattactct tagtcatata tctctttacc
    29341 accaaaaaca gaaaagccat tctcaccctt atacccatag gtattgcggc cgtagcatcg
    29401 gtcggtgtgg aagtggtact gaagatggag ttttccataa ttgaagacat gatgtatatt
    29461 gccagattcg catacaatgt ggtgatgttg ttctgctggt acaatatttt atcggtattg
    29521 ggatatgaga aagaacagct tcttaagaaa cttaacaaac tgttatgctg gactttaata
    29581 ataataggag taaatgtaat attcggtcag ataaccggaa cggggcatta cacttatgcc
    29641 gacagattcg gttacagagg ttccatggga tactatatag cgggcaatga tataacggcg
    29701 gtattaatgc tggttttccc gttatcgata tcggccttca tgcacaccga taaaacaaac
    29761 aaaaaagaga gaaactttta tcttgtcgct tcttcgatga ccgttgtaac aaacagcctg
    29821 ataggtacaa agaccagttt tttggcactg tttaccactt gtgccgtagt tattgtctac
    29881 ggaacagtaa tacttgttaa gaaaaaagaa agtctgtatt taaaaaaatt cggagtgata
    29941 atactttctt ctttgatttt agccgccgtc ataatcggtc tggaatttgc actgtcaaaa
    30001 gcatccttta tggaaaccat atcccaatct ttaaggagta ccggcattat cttaagaaga
    30061 gaaggattac agacggcact gttgagcggc agacaggtaa aattgaaaga agccttcatg
    30121 cagtttaagg aagcgggacc gttggcatgg gttttcggaa taggaagggg cagtcaggag
    30181 tttgtaatag agatggatgt actggaagtg ctgtgctact acggtatttt cggagcggtt
    30241 gcaatgctgt ggatttatgt cgcttcgggc tatatgctca taaagaactt tttgaagaat
    30301 gtcaacttcg acaccgttat ggctgttacg gccatagtgc tttgcaccgg ctatatgctt
    30361 atagcaggtc acattctgtt ttccgttact tccggttact attacaccat gatgtttgca
    30421 tatgcagata ttctgcacat agatgacata agagacctgc cggaaaggat accttttcct
    30481 tttaagaagc tgcttgaaag aaaaagtaga gtttaatttt ttcttaaggc taaccgatac
    30541 ggttttttcc tgtttacttt acggacagga taaaatataa taaccatagt agcgtttaaa
    30601 tttaattgac aatatggaac ctacggtgtt ttcattacgt caaataataa cagacgttcg
    30661 tacaaaagaa attcgtaact aataaggagg aacgataata atgagaaaga caaaaaaact
    30721 attagcagta attctggctg tttcgatgat tgctgttttg tttaccgctg ttccgacatc
    30781 ggctgcagaa ctgaccgatc cgcagaagct ggctgctatg aatctattca gaggcgacac
    30841 tgccgaaggt cttacagcaa catatcttgc aaaggccccc gacagaaaaa ctgccgccag
    30901 actgttgctc agatttaccg gtcttgaaga agcttccgat gcctatacag gtacggctac
    30961 tttccccgat gccacatcag caacggtata ctggcagccg atgatcggtt atctcaaagc
    31021 caatcccggt gcgggattcg gcggatatga agacggtact ttccgtccca acgatcctat
    31081 gaccgcacag gctttctaca aagttctgtt aaccattctg ggatataagc aggataccga
    31141 ctttacctgg gcacagacaa ttacctttgc agcttccaaa ggactttcca aaatagcagg
    31201 caaggcaact ttaacgaatg ccgatgcagc aacagctatg gtggaagctt tgaatgcaaa
    31261 taccaagtcc ggagaaaagt taattaatgc ccttatcaat gatgaaattg tcacacctac
    31321 acaggcagct gcagccggat ttaccgtaat tacttccatt acgggatatg aagctcagta
    31381 cacaatggga tacggtggag ccaatcctct tccggctacg gtaacggcag tttacaaaga
    31441 cggaaccaca gcttccgtag cggtcgcatg gagcgcattc tccacaaccg ttgaaggaac
    31501 atataacgtg acgggaacaa tcgaaggtta cggaacaatt actacaaaag taacgctgac
    31561 caagacactt accgtaaccg atgcatatcc ttcaagcaca aaggaaatta cggttatgtt
    31621 caacatgcct gttccgactt ccgcagcagt ttcatgcaaa ttcggagtat cggttcttca
    31681 gggcctcaca cagacatggt ccaatgacag aaaaatgttg aagatatcca gaacatacaa
    31741 cttcacgccg ggcacatatt caattaccgc tttaaattca acaaaagagt ttgttattga
    31801 aaacgagaaa cttgttcagg ttgaaatagg tgccataaat ctttatcctc aaccaggcgc
    31861 acaggatctc aatgttacat tctataatca gtacggttta ccgatgaatc ctctcaccat
    31921 gagtcttcag ttgtcggcat tcagcccgca aaaaccgact gtggtatttg caatgaataa
    31981 gacagccaca tcggtaacga taagcggagt ggagaatctt gaagttgaca atacagttaa
    32041 tgtgttcgta gtggataaca ataccgcaaa tgcggcaaca aaagttatta ctgttgctgc
    32101 agctcccaca ataactgcat tctctttcgg agaactgact attgccggtt ccgcaacaag
    32161 gatactgaca ggaacttccg gtcatcttat ttcagtcaat gcttatgacc agtatggcaa
    32221 ttcgtacaaa ctcagaacgg gaaatcagga tatatccaca gttgttgcgc ctacaattcc
    32281 tttacttct
//
LOCUS       DAOSOM010000023        31164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_1477_length_31164_cov_5.618856, whole genome shotgun sequence.
ACCESSION   DAOSOM010000023 DAOSOM010000000
VERSION     DAOSOM010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 31164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31164
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1477_length_31164_cov_5.618856"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PLF14_06490"
     CDS             <1..253
                     /locus_tag="PLF14_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT70973.1"
                     /translation="YILPETMKEPDMVEFTSVSSEWGSAGRKYVSDVMVEGIDTNEAL
                     KSYLTVVMGFNPQAVLDEMNSRLGKTSEQNYNAIWSKFN"
     gene            522..1313
                     /gene="rpsB"
                     /locus_tag="PLF14_06495"
                     /pseudo
     CDS             522..1313
                     /gene="rpsB"
                     /locus_tag="PLF14_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838265.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
     gene            1448..2095
                     /gene="tsf"
                     /locus_tag="PLF14_06500"
     CDS             1448..2095
                     /gene="tsf"
                     /locus_tag="PLF14_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOT70974.1"
                     /translation="MISAGLVKELREKTGVGMMECKKALVEANGDLEKAVTILRERGL
                     ANAEKKSGRIAAEGIVASYIHGGGRIGVLVEVNIETDFAAKNEDFQAYVKDIAMQIAA
                     SKPEYVKRDQVPTDIIENEKAILRTQALNEGKPEKVVDKMVEGRIEKYYKQVCLMDQA
                     YIRNPDISVQQLTNEIIAKIGENISVRRFTRYEMGEGLQKKEENFADEVAKQMNK"
     gene            2138..2839
                     /gene="pyrH"
                     /locus_tag="PLF14_06505"
     CDS             2138..2839
                     /gene="pyrH"
                     /locus_tag="PLF14_06505"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOT70975.1"
                     /translation="MDYKRILVKLSGEVFAGPKGENLDTEMLMQIAQALKILRDEGKE
                     IAVVIGAGNFIRGRSIKGIVRSRADSMGMLATVINCIALKDALEKTGLDAEVYSAISM
                     SEICKTYRRDDAVRDLSNGIITVLGGGTGNPYYSTDMAAVLRAIEINADVILFAKNVD
                     GVYDKDPKEHKDAVKYDRVTYSTILSRQLNVIDMAAAVMCNEFSLRSVLFELKKPMNI
                     VQAARGGNIGTIIEK"
     gene            2932..3453
                     /gene="frr"
                     /locus_tag="PLF14_06510"
     CDS             2932..3453
                     /gene="frr"
                     /locus_tag="PLF14_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003394838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOT70976.1"
                     /translation="MSQAIDAFSEQISVIRAGRANPSVLKNIYVDYYGQKTPLLQIGN
                     ISSPEPRLLVIQPWDTSIMKDIEKAIMASDLGINPQSDGRVIRLVFPILSQERRNELV
                     KRVKAYGEDAKVRIRNIRRDDMDKYKKQKKDSEITEDDYIIIEKDIQKLTDESTAKID
                     DIVKEKTDEIMEV"
     gene            3487..4197
                     /locus_tag="PLF14_06515"
     CDS             3487..4197
                     /locus_tag="PLF14_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOT70977.1"
                     /translation="MQLPQHIAIIMDGNGRWAKQRNMPVSYGHKCGADAIQNLIEQSD
                     ELGIKYITAYTFSTENWKRPKSEVAYLMTLLQDFLNDTSGRLERSNIRIKFIGSRDRI
                     KKKMVESMERIEKISANKTGLTFIIALDYGARQEITQAVKKICTDVKAGTLDISQIDE
                     QLISDYLYTKDIPDPDLIIRPSGEKRLSNFLLYQASYSELWYSDILWPDFTIEHLKQA
                     ITDYNGRKRRYGGRTDED"
     gene            4187..5017
                     /locus_tag="PLF14_06520"
     CDS             4187..5017
                     /locus_tag="PLF14_06520"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOT70978.1"
                     /translation="MKTRVISALFALSILVFIYVFPGLPTYIVLFLISALMFYEFLHV
                     TNEKVTVGYYIICSLFTLLYYANLYLTIGLSIIPQEYLYTLNILLTVFFALTILSASM
                     FYKIKTFREVTYTIFGYIYTVVLLSFVVLILKMEKGGLLFVYVWLGAFGCDIWAFLIG
                     KRFGKTKILNEVSPSKTLEGAIGGAVGSILMITAYTIIINRYFNAEIPWYVLVALYIT
                     CGILSQFGDWCASYIKRQFNVKDFGKIMPGHGGALDRLDSLLFILPFIYSIFLISEFK
                     "
     gene            5014..6147
                     /gene="dxr"
                     /locus_tag="PLF14_06525"
     CDS             5014..6147
                     /gene="dxr"
                     /locus_tag="PLF14_06525"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOT70979.1"
                     /translation="MMNVLILGCTGSIGENTVKVCKDIGYRIIGVTCKTSYTGLADII
                     ERYELGDNLKYIGISDNESCDKFKLLGLNLKAKVFGGEKANIHCIQSMKYDIIVNALN
                     GSNGIEPSYYAVSNCKRLALANKETIVAAGDIILDRAAQYSCQVVPVDSEHGAILQCI
                     EHNPIKRIYITASGGPFWNLDKAQLSKVTVEQALNHPVWKMGTKITIDSATMMNKGFE
                     VIEASKLYNLTKEKIKVLIHPQSIIHSMVEFYDNSIKAQLSTCDMKLHIQYALTYPER
                     INNGYEELDLTEYNLTFHKPDFDKFKCLKLAYEAVKEGNSLGTVINAANDECVEYFLK
                     GKIAFTDIADIIEKVMNKHDNIKAESIEDVLSISDWAKTEARI"
     gene            6144..7430
                     /gene="rseP"
                     /locus_tag="PLF14_06530"
     CDS             6144..7430
                     /gene="rseP"
                     /locus_tag="PLF14_06530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOT70980.1"
                     /translation="MSTFTSILIALLALSIIIIVHESGHFFAAKISGINVLEFSLFMG
                     PKIFSKKIKGTVYSIRAIPIGGFVALEGEEDNVVSPTSYSSAKPYKKIFVSVAGPLSN
                     IIFSFLILTILFSFTGYALDTNTVIVPDGPLYEAGLRDGDKIISVDKKAIIDPSDINM
                     VLYPLKEAEEIEVVYEREGIKNIVTVVPVKQGGKRIMLNISTTNADTVIVEIKEDSNG
                     YIAGLRLNDKITHINDIKINSPRELREQVNANGEGPVKVTVLRGEEKLSFVFDTIETE
                     STEYLDLGIYYYSTEINVLQAIKHAVLYSFSVVKTVIVSIVWLITGIVKMSEMMGPIG
                     IISTMGEQIKQPTFVLFMLNLVNMMAFISINLGIMNLIPFPALDGSKIVIHSIEWIMG
                     KPFNQDKMKYVTLAGFVLLILLLVFVTFNDIVRLFK"
     gene            7427..8491
                     /gene="ispG"
                     /locus_tag="PLF14_06535"
                     /gene_synonym="gcpE"
     CDS             7427..8491
                     /gene="ispG"
                     /locus_tag="PLF14_06535"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOT70981.1"
                     /translation="MKTFNEKRKVKKEINIGDITIGGKNDIAIQSMTNTDTRDLNATV
                     NQIARLKDAGCDIVRISVFDNDCVSNIPLIKKAVEVPLVADIHYNHILAIESIKAGID
                     KIRINPGNITDKNDIKAIINTAKEYKVPIRIGVNTGSLPEHLYDMYNGPTSEAVLHAV
                     KDQVDMFLNYDFDNIVIAAKSVDAMQTVEIYRKLNEAFDFPLHTGVTEAGSYIKGTIK
                     STVALSILLDEGISDTIRISLTDDPVKEVTVAKDILKFTGIKEYGVNIISCPTCGRTK
                     IDIIKIASIIEERTKHIKKNIVIAVMGCAVNGINEAKYADIGIAGGDGKAVLFKKGKR
                     LRTIPEDRIIEELLIEIEKC"
     gene            8485..11754
                     /locus_tag="PLF14_06540"
     CDS             8485..11754
                     /locus_tag="PLF14_06540"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOT70982.1"
                     /translation="MLSDDINIIKFRYEKENHTLFIELSVKRYIFFREETALKKDIRS
                     EYNIGKEFSDIVFDFIDVTFNKEKTDEYIEDIYENLFNEFNYLILYKSTMNYEIKSNT
                     VYIFLPKEASMIADMSKINAYLFKCSKRLGNDVRFIISETVNEDATKIRDASIAGIRN
                     EKPEVVMPQPEEEVIEQPSGRKITRICELDSNSYKVVIEGTITYVKIHKNQGYQLFEF
                     GVNDLSGSIVCKRFFSREKDRNIIEKYETIIVENKYVSVSGKHQIEKNSPRYFINADK
                     VTEVKLGVRTDKARIKRIEMNIDTDHVNADLLLEQLKRWKHKAFGIMPKENIQSFVDI
                     YSKNFVNRQKLKMILGVTLNVLMDDNTAIFNPYKTEISQNICAGYVKEDNIFISDISD
                     KTAKRKEVFKKNDYIGIRNFCEQKALYIFSEADDDYVATMLAKIGITVINVKRLFEIL
                     EKKTFTVNTLLLRLNASSDNIQSAAYNVSGILNKSFDTYEEICINIANNNVKNENMSV
                     YPLSVTVKNKEGLRNLYKILTKRNCIWSIVPRSYLEAHRHGLLLGSSDADGELYSMLL
                     QDAGTESIYAKAAFYDYVNLLTDRHAELLKEKNIIKTINEFRNFNKFLYKCATECNVI
                     PIATCFSKDISKIEFMTTNEMLSEFSYLEKENAYKAVVENTVLLDAKIENVPPIIKSD
                     YKDDIISIAYLTNMNDKTEAYEKEFKMITSEKTAGILTVIKQLIESLKDKDIMFRIES
                     TPAPYILSKLLNDDSYNYLLKNDFKIHVQNDAYDDVYEILKELCTDHRPFSVKYDRYT
                     AIHLLPLEHTIYDFSPMVMRNNEMVTLYDNEVLRKYFSTFILQADEDMQMISNLLKST
                     NVYPTDDLIGAMTYDDIGSITTDSKTHDILTMIKPASSMEFVRCVGLIYGQGCWYDNA
                     ERLIHERLTDIYDVICFKHELDDEKKDDDMHKESKRKIKYCITYEEALIHSKNMMILK
                     WYHDNYPKQFQFEYINRYRDLDTPENHFIIKKADISDSLKSRYLHDEELTAITQPLDV
                     ITYLDDKMIDNIIINRPYSSVDDFALRNRIDSELLEKLKTDGYLDSLDKTDQILITDI
                     C"
     gene            11861..12316
                     /locus_tag="PLF14_06545"
     CDS             11861..12316
                     /locus_tag="PLF14_06545"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HOT70983.1"
                     /translation="MSKSIAKQALEIAAGHAKKMNIEVLDAEYVKEGNERYLRVYLYK
                     PEGISVDDCEFVHKAIDKEIDDLDIRESYYLEVSSPGYDKNLRTEREFEIYMNSEVEV
                     SLYKAMDNQKKFTGTLKDRDSENTTLLINDSLKIFDNKNIAKITRAFKL"
     gene            12327..13529
                     /gene="nusA"
                     /locus_tag="PLF14_06550"
     CDS             12327..13529
                     /gene="nusA"
                     /locus_tag="PLF14_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOT70984.1"
                     /translation="MKRESVSDSRQNTDLIDAIKQIERERGIDSETLIEAIEAALASA
                     YKKHTKTEQNIEVSLDVNTGQVKLFKIKNIVDEIEDEENDMLIDEARQYADVEVGYCL
                     KFEEEMVPRDFGRIAAQTAKQVILQRIKDAERIKILDIYENKLKEIVYGVVRRVENDT
                     VYLDLGNSEAIMPKKEQVPSERYGVNFKFKAFVYDVKKTSKEPKIFVSRTHPDLIKRL
                     FEMEVPEIQSGIVEIKDIAREPGARTKIAVYSNDPNVDATGACIGHKGIRVSNVVEEL
                     RGEKIDIIEWSPNPAQYISSALSPADVSRVDVIEVVNAEGESEIKARVTVPDNQLSLA
                     IGKEGQNARLAAKLTNWKIDIIPETELRRIIEEQLLSDDLLAKDTKPVSELDALFDHP
                     AEKEKDEE"
     gene            13519..13788
                     /locus_tag="PLF14_06555"
     CDS             13519..13788
                     /locus_tag="PLF14_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002847090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HOT70985.1"
                     /translation="MKSKKIPMRMCLSCRIMKPKKELIRIVLTEENKATMDLTGKKNG
                     RGAYICDDLQCLNKAKKSKSIERTFSIEHDDTIYNEMEEYINRKE"
     gene            13799..14086
                     /locus_tag="PLF14_06560"
     CDS             13799..14086
                     /locus_tag="PLF14_06560"
                     /inference="COORDINATES: protein motif:HMM:NF013417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HOT70986.1"
                     /translation="MIGLAIKAGAAVTGTSGCITQIKKKRIMLVFMAHDCSDNTREKI
                     LRAIKEKNIEISEMFSKEQLGSIINKEEISVIGISDVNFAKGISDKITKEN"
     gene            14087..16960
                     /gene="infB"
                     /locus_tag="PLF14_06565"
     CDS             14087..16960
                     /gene="infB"
                     /locus_tag="PLF14_06565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOT70987.1"
                     /translation="MGKRVNELAKEIDKKPSEVLRKLEQMGIESNVNTVLTDEQVQMI
                     YDIYRIKNPATGNKVRATVITSSDAASPVKTVVVKAPVKPKTKTEHKDESDKPMVVVV
                     KTTKGGETKSTIRHIKQEPQKSPETKKETPQKPQEKKVVVVKTPEVTVPQKESAGQTA
                     KPLKAEEQEIKKPQVAAPQKTEQKTSDKEVITKKEDKKEKTYIEQKAYKDKTFIPKER
                     TDKPYPPRDTRTDKGSYQQRDNRTDRPYAQKPGDKKSGYTRTDLHDGKQKPAARRTID
                     RDSDDDTSTRIEKPRVIKKEYIKKDQEKVLNKETKKETAVARSMASKQTKYEKREILT
                     PKGAIDTFMLTDDELFSTFYRDGGTRRKRKKRDASQPKIEVLKFVKLPPEMTVKEFAE
                     TIKKTGSDVLSKLLDMGSGIMSINESIDFDTAALIADEFGITCEMENIITEEDILFDD
                     DKECDPKDLVERPPVVVVMGHVDHGKTSLLDYIKNSNVAATEAGRITQHIGAYRVKIG
                     ERYITFLDTPGHEAFTAMRKRGADVTDIAIITVAADDGIMPQTIEAINHAKAANVNII
                     IAINKIDLPTANPDRVMQQLMEHNLVATKYGGDIECIPVSAKTGEGIDTLLDTILLSA
                     DILELKANPKRQAKGIVLESRVERAGTVTTLLVQRGTLKKTDYIISGTSVGNIRALTD
                     DRGNSIDSAGPSMPVEILGLDTPPEAGEIFYAVDDERTAKSLAERRKYERQMAKTAHK
                     LTTLDDLYEQIKAGNIKDLNLIVKADVQGSVEALTDSFVKLSGDKVKVHIIHAAVGTI
                     TETDVALAEVTNSIIIGFNVRPLGNVTDLAKEASVDIRLYSIIYDAIEDVKKAMAGML
                     EPLTKEVVYGHAQVRETFKITGVGTVAGCMVLDGKILRNTNVRLVRDGITIFDGALSS
                     LKRFKDDVKEVAAGYECGMSIEKFNDIKIDDIIECYKIEVVKK"
     gene            16957..17319
                     /gene="rbfA"
                     /locus_tag="PLF14_06570"
     CDS             16957..17319
                     /gene="rbfA"
                     /locus_tag="PLF14_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012423722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOT70988.1"
                     /translation="MKTRRVEQLNSEFKREISSILADEIKDPRLTAIVSVLNVNVTRD
                     LSYADIHVSMIGTEEQKKESFEAILSSAGYIRRLLGQRMKIRYIPQLRFKYNDYIEEG
                     IRISKLIDEVIKDSKATE"
     gene            17285..18271
                     /locus_tag="PLF14_06575"
     CDS             17285..18271
                     /locus_tag="PLF14_06575"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOT70989.1"
                     /translation="MKLSKIAKQLNKDIKKYDSFLILPHIMADGDTIGCCIAMARYIT
                     SRGKKALMLLEEKIPYIYSFMEDSCDMEVYVPDKEYNFDVCIVLDTADINRLGRRKSF
                     LKNKVSYNIDHHKTNDGYAMYNFINADVSSVGEIIYDLFTKMKASIDHDIAQAIYVAI
                     ATDTGGFRYQNTNKNCFIISSRLMDYDINVEKISEWVFERTSMNKLYLFKNTIKNIKL
                     YFDNRVAISVLRREEYMHLDVADEDFEGLVNVVKNIEGVEVGVFLRERNSDREVKGSL
                     RSNTDEIDVSVIAKTFGGGGHARAAGLSCNMTLDQVYDGMIEKLNEVYGCTE"
     gene            18259..19158
                     /gene="truB"
                     /locus_tag="PLF14_06580"
     CDS             18259..19158
                     /gene="truB"
                     /locus_tag="PLF14_06580"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOT70990.1"
                     /translation="MYGIINLYKEKGLTSFKQLNMLKSVFPGIKIGHTGTLDPIAEGV
                     LVVCIGKATRIADYIQAQKKVYRAEMFLGVSTDSYDITGKILSINSSDDISETAILNT
                     IKLFIGKQWQKPPVFSAKKVKGKKLYQYAVNQQPVEIKASYIEIQNISNILINDYIYE
                     NTPVKKISFTVECSRGTYIRSLCHDIGEMLKKGGTMSALTRLQVGNFKITDSYTVDEI
                     LKKASENNLQTIFTPIENAIDINDIYLTADEIKKYINGVKIEKKLKKGEYIIKDVNDT
                     VIGIGYIDENNILKGKILLNEDY"
     gene            19145..20074
                     /locus_tag="PLF14_06585"
     CDS             19145..20074
                     /locus_tag="PLF14_06585"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOT70991.1"
                     /translation="MKIIKSEDNYIFEKSNVIALGNFDGLHIAHRKLIETAVSIARKN
                     NLLSLVYVFDIHPMTIVGNTKQRLITNLEQKLELISGLGVDIIYIQKFDYEFSKMQPE
                     HFITDILKKRLSCAYAVSGYDYKFGYKAEGDTDTIKNIGSKHDIKVKILERIDYLGQP
                     VSASRIRELLEIGDIQYVNNLLGREYQIRGTVEKGKLIGSKIGYPTANIILKDEMQIP
                     AFGVYQTVTTVDGIGYDSITNIGNNPTVNKEDIDKITCETHIIDKFGLKLYGKKIYID
                     FIKMIRKEQKYPDIEALKKAIARDILLVKSYYK"
     gene            20128..21216
                     /gene="lgt"
                     /locus_tag="PLF14_06590"
     CDS             20128..21216
                     /gene="lgt"
                     /locus_tag="PLF14_06590"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOT70992.1"
                     /translation="MKSIIAFPNLFGGIELSVSRIAFSIGKFEVYWYGLIIAIAVCLA
                     VVLVLKNAKKYGINEDDLLNICLIGIIVGVICSRLLYVLGRLDRDWTFADILNVRDGG
                     MSIYGALIGGFIATAIYCKIRKMNFLKLADLVVPYIALAQAIGRWGNFINKEAFGVPV
                     NPNSIFSTLAMTSMKIGGNVAGSIEEELLKLGFVDTSLVATVNGNTFDYIGVHPTFFY
                     ESVLNLILFLVLILVARNGKYFNGRSLCIYMASYGIYRFFIEGIRIDPLYLFDTDIKL
                     SRLLALISAIFFGTLLIIISILRAKDKKLFIKALLDEEETDIGKSDYKDLLVDKDTYV
                     SENNTEETTEKEESEKDIVSEEEQSVDK"
     gene            21243..21319
                     /locus_tag="PLF14_06595"
     tRNA            21243..21319
                     /locus_tag="PLF14_06595"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21277..21279,aa:Pro,seq:ggg)
     gene            21375..21917
                     /locus_tag="PLF14_06600"
     CDS             21375..21917
                     /locus_tag="PLF14_06600"
                     /inference="COORDINATES: protein motif:HMM:NF021561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HOT70993.1"
                     /translation="MQYNVEEFKKLAISMGFSNAGQLNMDALVFMPEVRQMCNSDSCT
                     SYNKNWMCPPAIGTLEQSAVKVREYSFGMIVQTTAFMQDEFDYETIRDTQKKHKNNFF
                     ELVTLLRNKYSSVLPMGAGTCTICNKCTYPDKPCRFPDKAIGSMESYGLWVSKVCEMS
                     SIPYYYGKNTLTFTSCFLLK"
     gene            22103..22747
                     /locus_tag="PLF14_06605"
     CDS             22103..22747
                     /locus_tag="PLF14_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOT70994.1"
                     /translation="MISEIELLENNKIYIAGSIASAPVFNHQIYGESFYNIIVEVQRL
                     SKQTDMVPVLISSHLLDIDLLKPDTLIEINGQVRSYNNIDKNEKKRLVVNVFAREARI
                     LQTLEGIESFNSVFFDSFICKEPNFRITPFGREICDLLIAVNRAYKKSDYIPCICWGR
                     NARYCENMKVGTRLRIWGRFQSREYEKKDHYGNIVTKTAYEVSVIKLEKVEENK"
     gene            22744..24006
                     /locus_tag="PLF14_06610"
     CDS             22744..24006
                     /locus_tag="PLF14_06610"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010272618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOT70995.1"
                     /translation="MKINIDDCIRFSDGKKFESMLRKAKKADRLLKSGKGAGSDYLGW
                     LDLPKRIKKEDIKLINKAAAKIQQQSDVLVVIGIGGSYLGARAAIEALTDVRNIKGTE
                     VIFVGNNISGKYVESVLEYLKDKDYSLNVISKSGTTVEPAIAFRIFKEALYEKYKEKA
                     KDRIYITTDPVKGALRSIVEKEGYTSFEIPADIGGRYSILTPCGLLPIAVAGIDIEQM
                     ISGAKDALKNCTEMSVTYAAARNLMYKSKKTIEIMVNYDPSMHFFTEWWKQLYGESEG
                     KENKGIFPAGVDNTTDLHSMGQYIQEGIRNIFETVVYIKDDMSTITVPDISDNSDGLD
                     YIKNKSLKYVNEMAMRGTKSAHLEGDVPVILIELEKMDAFTFGKLIYFFERACGISGY
                     ILGVNPFDQPGVEAYKKSMFKLLGKPGY"
     gene            24009..25115
                     /locus_tag="PLF14_06615"
     CDS             24009..25115
                     /locus_tag="PLF14_06615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOT70996.1"
                     /translation="MTENEKSVKKAKKTNTRKLSSKVIFILSLVTLFVICIIISLIIF
                     FNSMHTSIYETPSPTPSKVAETITLPPSETTSTEPVETQVTETPAPTPIPMYKPQDDI
                     EFLLLFGLAEYDLADTIWIGIVNKTDNKIDFLSIPRDTYLRYKDRNNPDHWKINSYYN
                     NYVNDQWVKTSDSASSMYLMGACEALLNIDMDYYVRVSYDTIEKIVDSMGGIEYDVPF
                     RMEYYDLTPGHELIIEIDEGLQVLNGENVVKFLRFRQGTGYDYISDIARINRQQDMVK
                     TIIKKAFTLSNISAVIEVAKNNIRTNFGKDQLTSFITFSLSLNSEKISFHTLRGYEDM
                     VNTQVKDSDGNDIIRSFYIYSDEEVRKQLISICE"
     gene            25281..26543
                     /gene="hisS"
                     /locus_tag="PLF14_06620"
     CDS             25281..26543
                     /gene="hisS"
                     /locus_tag="PLF14_06620"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOT70997.1"
                     /translation="MKISIPKGTKDILPGEIEKYKYVQTAFADMLENFGYSQIRFPVF
                     EYTELFQRGVGDTTDIVQKEMYTFNDKGGRSVTLRPEGTASVVRSYIENGMSSNTQPV
                     KLYYDITAYRYENVQKGRYREFNQLGAEILGSDSCNADVEVLFMLYSFFNGLGIQGLH
                     LNINSIGCKTCRAEYNSILKEYFSDKTDNMCADCKRRYITNPMRLLDCKVDKCIEIAK
                     DAPMLLDNLCDNCKEHFYNVTAGLDEINIPYIINKRIVRGLDYYVRTVFEFIAEGLGA
                     QNTVCAGGRYDGLVEQLGGTDTPGIGFAMGLERLVETMKNSGFKFPTAKTPVIFIGYI
                     GEKAFKVARTIANELRLKGIYCEIDINDRSLKAQLKFADKKDALYSVVLGDAEIDTNK
                     AIIKNMKTQETKEVSIDSIVRRVVENII"
     gene            26552..28339
                     /gene="aspS"
                     /locus_tag="PLF14_06625"
     CDS             26552..28339
                     /gene="aspS"
                     /locus_tag="PLF14_06625"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOT70998.1"
                     /translation="MENIKGLTRTGYCAEYDIKDIGKKVVLMGWVNRSRNLGSIIFCD
                     MRDRTGLIQVVFREEKNKSLFNKATNLKSEYVIAVTGIVNEREDKNPNIKTGDIEIEA
                     EELRILSESAVPAIDIAENTATNELMRLKYRYLDLRKPKQQYYLIMRNKVAKCTRDFF
                     SENGFIEVETPVLTRSTPEGARDYLVPSRVNQGKFYALPQSPQLLKQLLMISGLDRYY
                     QIVKCFRDEDLRANRQPEFTQIDLEMSFVDVDDVIAVNEKFIKKLFKETLNVDVNIPF
                     ARMTYEEAMNRFGSDKPDLRYGMELTDISELVKDSEFKVFSDACKNNGSVRGINAKGC
                     ADFSRKDIDKLTDFVKDYRAKGLAWLKVGVDNEITGSIVKFFDKEQLTSIVDAFNGEE
                     NDLILIIADRNETVFDSLGALRTEIAKRMNLADHERWEFLWVTEFPLLEFDEETNRYY
                     AKHHPFTSPMDEDIPLLHTHPEKVRAKAYDIVLNGEEIGGGSIRIHDSVFQEEMLEVL
                     GFTKDKAQESFGFLLNALKYGAPPHGGMAYGLDRLVMSITKTDNIRDVIAFPKIQSGA
                     CPLTDAPNIVEKKQLDELGICLKERKDEV"
     gene            28329..29345
                     /locus_tag="PLF14_06630"
     CDS             28329..29345
                     /locus_tag="PLF14_06630"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HOT70999.1"
                     /translation="MRFKCYLKLLSVFIILLSLIFISSCDIVKFPVYVYGLIDTEGNY
                     LVQPEFTGIKIGKEGLYPIRQGINEYYKWGFCDKNGNIVIEPQYRDASMFSEGMAPVQ
                     VYDQSELWIFIDKENNQVFEKQFKYALPFSEGLACVQSSARDSTYNLWGYIDKTGEYV
                     IEPQFQIAASFSEGLAKIQTGSGSAGLCGYIDKTGEIVIEPQFSYAGLFSGGLASVKL
                     SMDQETNDAGYIDRQGNIVIPLIYYNTSTFSDGLAPVLAGETFKDALYGYINVDNEMV
                     IKPKYYMAYSFSEGLAAVKTDYYENSGWMYIDTEGKLVIKNDYSSASSFIDGVAPVGM
                     VEYR"
     gene            29342..>31164
                     /gene="recJ"
                     /locus_tag="PLF14_06635"
     CDS             29342..>31164
                     /gene="recJ"
                     /locus_tag="PLF14_06635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOT71000.1"
                     /translation="MMFNNQWDLSNIVDISNTNLAQSILKRRNIEDLNTYLYPKLSDL
                     HDPFLLKDMDKAVERIMRAIEDKELILIYGDYDTDGITATAVIYRFLKNLDAEATYYI
                     PKRSEGYGLITEVLENILNEDIGLIITVDCGIPSVKEVDFINEIGIDCIISDHHTVQE
                     EIPDAYCIISPKVSKSYPFNELSGVGIALKIVQALSIKLNNPQEYAKYIDLAALGTIA
                     DQVPLIDENRTIAKIGMDSIPFTQNVGLKELISQSYPNGINKVNSSNLMFNIIPKINA
                     SGRMESPEYAIKLLISDDYEECRKLSEKLIQLNEERKILQKEITDSVLDKIESNESIK
                     NDSVIVLYNEQWEIGILGIVASKIVDKYNRPCILLGGNEKYFGEERIIKGTGRSVEGF
                     NLIEALIHCHSFLDKDVETCGGHYFAAGNELKESSVDKFRNIINQYAKDKGFTVSERK
                     LLSPDYKVNISDINADNARILSCMEPYGSENELNLFYIEDMILANWRLIGKTKDHMTM
                     SFKKDGRYMNCISFNSAKYSKLLLNGLSYDLVFKMSLNTVRDSNSFTKDVRYYVNCEI
                     ADMRIKDIPLAIMDDLVKYSKSDANIEREDIAKVYRLIRSNT"
BASE COUNT        11365 a         4636 c         6703 g         8460 t
ORIGIN      
        1 gtatatactt cctgaaacaa tgaaagagcc tgatatggta gaattcacat ctgtaagcag
       61 tgagtggggt tcagcaggaa gaaagtatgt atcagacgtt atggttgaag gcattgacac
      121 aaacgaagct ctcaaatcat atctcacagt tgttatggga tttaatccgc aggcagtgct
      181 ggatgagatg aactcaagat taggaaagac ttcagagcag aactataatg ctatctggtc
      241 taagtttaac taaacaaaac aatgagaaaa gactcgggaa accgggtctt tttttatatg
      301 aataaaatat tattaatgtg aatttattat ataatattta agaaataatt tcataaattt
      361 aaaatactgc ctactgtaac ttagaaatac ggtattgcat aatatccgat gataatgtat
      421 aattcactgg aaaataacac atgtcggtga atttatgtat gttgcgttaa tcgttgtaca
      481 taaagttgaa gccggcagag gaaaaaacgg aggattaata catgtcagtt atttcaatga
      541 aacaactatt ggaggctgga gttcatttcg gtcatcaaac aagaagatgg aatcccaaga
      601 tggctgaata catctttacc gaaagaaacg gaattcacat tatcgattta caaaagaccg
      661 taaaacaaat agagaaggca tatgatttcg taaaacaaat agttcttgac ggtggagaag
      721 tattatttgt gggaacaaaa aaacaggcac aagagtgtgt tgaagaagaa gcaatcagat
      781 gcggtatgtt ctatatcaac acaagatggc tgggaggaac tctgacaaac tataagacaa
      841 tggagaagcg gattgagtat ctcaagaaac ttcatcagat ggaagaggac ggtacatttg
      901 attcgttacc gaagaaagaa gtaattaagc taagagcgga aatggaaaaa ctggaaaaaa
      961 acttaggcgg aatcaaagat atgcacggaa ttcccaatgt gctttttgtc gtagatccta
     1021 aaaaagagcg taatgctgta ttggaagcga aaaaactgga tatccccgta gtggcaatta
     1081 tagacaccaa ttgcgatccc gatgaggttg attatgttat tccgggtaat gatgacgcaa
     1141 taagagccgt taaacttatt acaagtgtaa ttgcaaatgc cgttattgaa gcaaatcaag
     1201 gtgaagcatt ctctgttgaa gatgaagaag agattgatat gtcgcaactc actgaagaag
     1261 agcttctgaa ggcaaagcat gatgataaaa aatttaatga gccaaaaaaa gaatctaaaa
     1321 aagccgaaaa aaaagatgaa tctttgaatg aaatagttca ggaggctgaa gcatcaattg
     1381 aagaaatagc agaagttgct gaagaagtaa ttgaaattgc cgaagaatag tacatggagg
     1441 tagtatgatg ataagtgcag gtttggttaa agaattacgt gaaaaaaccg gagtcggtat
     1501 gatggagtgc aaaaaggctc ttgtagaggc aaacggtgat ttggaaaaag ccgttacaat
     1561 tttgagagaa agaggtttgg ctaatgccga aaagaaatcc ggaagaatag ccgctgaagg
     1621 tattgttgca tcatatatcc atggcggcgg aagaataggt gtacttgtag aagtaaatat
     1681 tgaaacggat tttgccgcta agaatgaaga tttccaagca tatgtaaagg atatagcgat
     1741 gcagatagca gcttcaaaac cggagtatgt aaagagagat caggttccga cggatattat
     1801 tgaaaacgag aaagcaattc tacgtactca ggcattaaat gaaggtaaac cggaaaaagt
     1861 tgttgataag atggttgaag gcagaataga aaaatactat aaacaggtat gtcttatgga
     1921 tcaggcctac atcagaaacc cggatatctc cgttcaacag ttgacaaatg aaataattgc
     1981 taagatagga gaaaatattt cggtaagaag atttaccaga tatgaaatgg gtgaagggct
     2041 gcagaagaaa gaagagaatt ttgctgacga agtagcaaaa caaatgaata aataatacta
     2101 aaggcactta tatgtgcctt ttaaattgga gaatgatatg gattataaaa ggatactggt
     2161 taaactgagc ggagaggttt ttgccggacc gaagggagaa aacctggata ccgagatgct
     2221 gatgcagatt gcacaagcat taaaaattct gagggatgaa ggaaaagaaa tagctgtcgt
     2281 tataggtgcc ggcaatttta taagaggaag aagtataaaa ggtattgtaa ggtccagagc
     2341 ggattccatg ggtatgttgg caaccgttat taactgcata gcattgaaag atgcactgga
     2401 gaaaacaggt ctggatgctg aagtctattc ggccataagt atgagcgaaa tttgcaaaac
     2461 ctacaggaga gacgatgctg tacgtgattt aagtaacggt attataactg tactgggcgg
     2521 aggcaccgga aacccgtatt attcaaccga tatggcggca gttttacgtg ctatagaaat
     2581 taatgccgat gttatactat ttgccaagaa tgttgacggt gtgtatgata aagaccccaa
     2641 ggaacacaaa gatgcggtca aatatgacag agtcacttat tcgacaatcc tctctcgtca
     2701 actgaatgta atagatatgg ctgctgccgt tatgtgtaat gagttttcct tgagatcggt
     2761 ccttttcgag cttaagaaac ccatgaatat agtccaagca gcaagaggcg gaaatatagg
     2821 gactattatt gaaaaataac ttcatattgt gtaaaatata tctaatatat taaacataaa
     2881 ggagttattg ccatgattag taaagatgaa tacagcaaaa ctacggaatt gatgtctcag
     2941 gcaattgatg cattcagcga acagatttca gtcatacgtg ccggacgagc aaatccgtct
     3001 gttctcaaaa atatatatgt ggattattac ggtcagaaga caccgttact gcagataggc
     3061 aatatttctt ctccggaacc caggttgttg gttattcaac cttgggatac aagcattatg
     3121 aaagacatag agaaggccat aatggcttcg gatttaggta ttaatccgca aagtgacgga
     3181 agagtaattc gtctggtatt cccgatatta tcgcaggaaa gaagaaacga gttagtgaaa
     3241 agagtcaaag catatggtga agatgcaaaa gtaaggataa gaaacatacg cagagacgat
     3301 atggacaagt ataagaagca gaaaaaggat tccgaaatta cagaggatga ctatattatt
     3361 attgaaaaag atatacaaaa acttacagac gaatctacag caaagattga tgatatagta
     3421 aaagaaaaga ccgatgagat aatggaagtc tgatatctct tacggaaacg gagaatttaa
     3481 cgttatatgc aattaccgca acatatcgcc attattatgg acggaaacgg aagatgggca
     3541 aaacagagaa atatgccggt cagttacgga cataagtgtg gtgcagatgc aatacaaaat
     3601 ctgatagagc aaagcgacga gttgggaata aaatatatta cggcttatac tttttctacg
     3661 gaaaattgga aacgtcctaa aagtgaagtt gcctatctga tgacgttgct tcaggatttt
     3721 ttgaatgata catcaggtcg tctggagaga tcaaatatac gtattaaatt catcggttca
     3781 agggacagga taaaaaagaa gatggttgag agcatggaaa ggattgaaaa aatatccgct
     3841 aacaagaccg gtcttacttt cataatcgca ttggattacg gtgccagaca ggagataact
     3901 caagcggtaa agaaaatatg tacagatgta aaagccggaa ctttagacat ttcgcaaatt
     3961 gatgagcaac tgataagtga ttatttatac acaaaggata ttcccgatcc cgaccttata
     4021 atacgcccga gcggagaaaa gcgattatcc aattttctgt tatatcaagc atcatactcg
     4081 gagttgtggt attcggatat tttatggccg gatttcacta tcgaacatct gaaacaggcc
     4141 ataacggatt acaacggcag gaagagacga tatggaggaa gaacggatga agactagagt
     4201 tataagcgct ttgtttgcgt tatctatatt agtgtttata tatgttttcc ccggattgcc
     4261 cacatatata gttctttttt tgatatccgc attgatgttt tatgaatttc ttcatgtgac
     4321 aaacgaaaaa gttactgtgg gatattatat tatttgttct ttattcactc ttttatacta
     4381 tgcaaatctc tatctgacca tcgggttatc aataatacct caggaatatc tgtatacact
     4441 taatatttta ctgacagtct tttttgcact tacaattctg tcagcatcaa tgttctataa
     4501 aataaagaca ttcagagaag ttacatatac cattttcggt tacatatata ccgttgtatt
     4561 actatcgttt gttgtcctta tactgaagat ggaaaaaggc ggattgctgt ttgtctatgt
     4621 atggttggga gctttcggat gtgatatatg ggcttttctc atcggtaaaa gattcggtaa
     4681 aaccaagata cttaatgaag taagcccgag taagacattg gaaggggcga taggaggagc
     4741 ggtaggctcc atattaatga taaccgctta tacaataata attaacagat acttcaatgc
     4801 ggaaatacct tggtatgtgc ttgtagcatt gtatataaca tgcggtatac ttagtcagtt
     4861 cggcgactgg tgtgcttcat atataaaaag gcaattcaat gtaaaggatt tcggaaagat
     4921 aatgcccggg catggaggag cgctggacag attggactcc ttattattca ttttgccgtt
     4981 catctattca atctttctca tatcggagtt taaatgatga acgttctaat cttaggttgc
     5041 acaggttcaa taggggaaaa taccgttaaa gtctgtaagg atatcggata caggataatc
     5101 ggtgttacct gcaaaacaag ttataccggg cttgcggata ttattgaaag gtatgaactt
     5161 ggagataatc tgaaatatat aggtatttcc gataacgaaa gttgtgacaa atttaaatta
     5221 ttgggattga acttaaaggc gaaggttttc ggcggtgaaa aagctaatat tcattgtata
     5281 caaagtatga agtatgacat cattgtcaat gctctaaacg gcagtaacgg tatagaacct
     5341 tcatactatg ccgtatccaa ttgtaagcgg cttgcattgg caaataagga gacaatcgta
     5401 gcggcaggag atataattct tgacagagcg gcacaatatt cctgtcaggt tgtgcccgtc
     5461 gactcggaac acggagcaat cttgcaatgt atagagcaca atccgataaa aaggatttat
     5521 ataaccgcaa gcggaggtcc gttctggaat ttggacaaag cacaattatc gaaagtaacc
     5581 gtagagcagg cattgaatca tcctgtatgg aaaatgggga caaaaattac cattgacagt
     5641 gcgactatga tgaataaagg ctttgaagtt attgaagcat cgaaactgta taatttgaca
     5701 aaggaaaaga taaaagtact catacatccg caaagtataa tacattccat ggtggagttt
     5761 tacgataaca gtataaaggc acaactgagc acatgcgata tgaaactgca tatacagtat
     5821 gcattaacat atcccgagcg aattaataac ggatatgagg agttggattt aactgaatat
     5881 aatcttactt tccataaacc ggattttgac aaatttaagt gtcttaaact ggcatatgaa
     5941 gctgtaaaag aaggtaattc gctcggaacg gtaataaatg cagccaatga tgaatgtgtc
     6001 gaatactttc tgaaaggaaa gatcgccttt accgacatag cggatattat tgagaaagta
     6061 atgaataaac atgataatat aaaagccgaa agcattgagg acgttttaag tataagcgat
     6121 tgggctaaaa cggaggcacg catatgagta ctttcaccag tatcttaatt gcactactgg
     6181 cgctcagcat aataataata gtacatgaat ctgggcattt cttcgcagcg aaaatatccg
     6241 gaattaatgt attggaattc agtcttttca tgggacctaa aattttcagt aaaaaaatta
     6301 aaggtacggt atacagtata agagctattc ccataggagg ttttgtcgca ctggaaggtg
     6361 aggaagataa tgtagtcagt ccgacatctt attcttccgc aaaaccgtat aaaaagattt
     6421 ttgtatcggt tgccggaccg ctatccaaca taattttttc atttttgatt cttacaattc
     6481 tattcagttt cacaggttat gctctggata caaatacggt cattgtaccg gacggaccgt
     6541 tatatgaagc gggcttaaga gacggcgata agattatatc cgtagataaa aaagcaataa
     6601 tagatccttc ggatattaac atggttcttt atcctctgaa agaagcggaa gagattgaag
     6661 tcgtgtatga acgagaaggc attaaaaata tcgtaacggt ggttccggta aagcagggag
     6721 gaaaacgtat aatgctcaat atttccacaa ctaacgccga taccgttatt gtggagataa
     6781 aagaagactc caacggatat attgccgggt tgagattaaa tgacaaaatc actcacatta
     6841 atgatattaa gattaattca ccgagagaat tacgcgaaca ggtaaatgcc aacggagaag
     6901 ggcctgttaa agtcaccgta ttaagaggtg aagaaaaatt gtcatttgtg tttgatacga
     6961 tagaaaccga atcaacggaa tatctggatc tgggaatata ttattacagt acggagatta
     7021 atgtattaca ggcgattaaa catgcggtat tgtactcctt ttccgttgtc aaaaccgtta
     7081 ttgtatcaat agtctggctt ataaccggaa ttgtgaaaat gtcggaaatg atgggaccta
     7141 taggtataat atccacaatg ggagaacaga taaagcaacc cacatttgtg ctgttcatgc
     7201 tgaatcttgt aaatatgatg gcatttatat ccataaatct gggaattatg aaccttatac
     7261 cttttcccgc acttgacggc tcaaagatag ttatacactc catcgaatgg ataatgggaa
     7321 aaccttttaa tcaggataag atgaagtatg ttacacttgc gggatttgtg ttgcttatac
     7381 tgctgcttgt atttgtcact tttaatgaca ttgtaaggtt gtttaaatga aaacttttaa
     7441 tgaaaaaaga aaagtaaaga aagaaataaa tatcggcgat attaccatag gcggaaaaaa
     7501 tgatatcgct attcagtcta tgacaaatac ggatacaaga gacctgaatg caaccgtaaa
     7561 tcagattgcc cgattaaaag atgctggttg tgatatagtc agaatatcgg tttttgataa
     7621 tgattgtgta agtaatattc cattgatcaa gaaagccgtt gaagtacctc ttgttgcgga
     7681 tattcactat aatcacatac tggcaataga aagtataaag gccggaattg ataaaatcag
     7741 gataaatccg ggtaatataa ccgataaaaa cgacataaaa gccattatta atactgcaaa
     7801 ggaatacaaa gttcctataa gaataggtgt aaatacaggt tctttgccag agcatttgta
     7861 cgatatgtat aacggtccga catctgaggc tgtattgcat gcagtaaaag accaggttga
     7921 tatgttctta aactatgatt ttgataatat tgtcatagcg gcaaaatcgg tcgatgcaat
     7981 gcagacggtg gaaatataca ggaagctcaa tgaagcattt gatttcccgc tgcacacagg
     8041 tgtaaccgag gcgggcagct atataaaagg gacaataaaa tctaccgttg cattatcaat
     8101 actgcttgac gaagggattt ccgatacaat aagaatctct ttaacggacg atccggttaa
     8161 agaagtaact gtagcgaaag atattctgaa atttacgggt attaaagagt acggagttaa
     8221 tataataagt tgtcctacat gcggaaggac taagattgat ataataaaaa tagcatcaat
     8281 cattgaagaa aggaccaagc atatcaaaaa aaatattgtt attgccgtta tgggatgcgc
     8341 ggttaacggt ataaatgagg cgaaatatgc cgatataggt atagccggcg gtgatggaaa
     8401 agcggtcctg tttaaaaaag gaaagagact gagaacaatt ccggaagaca gaattattga
     8461 agaactgctt attgagattg aaaaatgctg agtgacgaca ttaatatcat aaaattcaga
     8521 tatgaaaagg aaaatcatac tcttttcata gaattatctg taaagagata tatatttttc
     8581 cgggaagaaa ctgcattaaa aaaagatata aggtcggaat acaatatagg aaaggaattt
     8641 tccgatattg tttttgattt tattgatgta acatttaata aagaaaaaac agatgaatac
     8701 atagaagata tttatgaaaa tttgtttaat gagttcaatt atttgattct ctataaatct
     8761 acaatgaact atgaaataaa gagcaatacc gtatatattt tcctaccgaa agaggcaagt
     8821 atgattgccg atatgtccaa gataaatgcc tatcttttca agtgttcaaa acgtttggga
     8881 aatgacgtaa gattcattat ttctgaaacc gtcaatgaag atgcgactaa aataagggat
     8941 gcatcgattg ccggtatacg gaatgaaaaa cctgaggtag taatgcctca gcctgaagag
     9001 gaggtgattg aacagccgtc aggaaggaaa attacacgta tttgtgaact ggacagtaat
     9061 tcctacaaag tggtaataga aggtacaatc acttatgtga aaatacataa gaatcaagga
     9121 tatcaattgt ttgagttcgg tgtaaatgat ttaagcggtt ccatagtatg taaaaggttt
     9181 ttcagtagag aaaaagacag gaatataata gaaaaatacg aaacaattat tgtggaaaat
     9241 aaatatgtca gtgtttccgg aaaacatcag attgagaaga attcacccag atactttata
     9301 aatgcagaca aagtaaccga ggtcaaattg ggtgtcagaa ccgataaagc aagaattaaa
     9361 cgaattgaga tgaatattga taccgaccat gttaatgccg acttgctatt agaacaatta
     9421 aaaagatgga aacataaagc cttcggaata atgcctaaag aaaatattca atcatttgtg
     9481 gatatttaca gtaaaaattt tgttaacaga cagaaactga aaatgatttt aggggttacg
     9541 ctgaatgtat tgatggatga taataccgca atatttaatc catataagac agagatatca
     9601 caaaatatat gtgcaggtta tgtcaaagaa gataatattt tcattagcga tatatccgat
     9661 aaaacagcaa agagaaagga agtattcaaa aagaacgatt acatcggcat aagaaatttc
     9721 tgtgaacaaa aggctcttta tattttctcc gaagccgatg acgattatgt tgcgacaatg
     9781 ttggcaaaga tcggaataac cgttatcaat gtaaagagat tatttgaaat attggagaag
     9841 aagacattta cggtaaacac gctgctgctc agattaaacg catcatcgga taatattcaa
     9901 agcgctgcat acaatgtttc cggcatatta aataaatcct ttgatacata tgaagagata
     9961 tgtataaata ttgcaaacaa taatgttaag aatgagaata tgagtgttta tccgctttca
    10021 gttacagtaa agaacaaaga aggcttaaga aatctgtata aaatactgac aaaaagaaac
    10081 tgtatctgga gtatagttcc gaggagttat cttgaagcac acagacacgg tttgttgtta
    10141 ggatcttccg atgcggacgg tgaattatat tcaatgcttt tacaggatgc gggtaccgaa
    10201 agtatatatg ccaaagcggc tttctatgat tatgtcaatc tcctgacaga cagacatgca
    10261 gagttgctta aagaaaagaa tataattaaa acaataaatg aattcaggaa ttttaataaa
    10321 ttcttataca aatgtgcaac tgagtgtaat gttattccga tagccacatg tttttcaaag
    10381 gacatttcaa agatagaatt tatgactacc aacgaaatgt tatcggaatt ctcatatctg
    10441 gaaaaagaaa atgcgtacaa ggcagttgtt gaaaataccg tattactgga tgcaaaaata
    10501 gaaaatgttc ctcccattat caaatcggat tataaggatg atattatcag tatcgcatat
    10561 ttgaccaaca tgaatgataa gacggaagca tatgagaaag aatttaagat gataacctcg
    10621 gaaaaaaccg cagggatttt aacggttatt aaacaactga tagagagttt aaaagataaa
    10681 gacataatgt tccgtatcga atcgactcct gcaccgtata ttttaagtaa attattaaac
    10741 gatgacagct ataattatct tcttaaaaat gatttcaaga tacatgttca aaatgatgca
    10801 tatgatgatg tatatgaaat tctaaaagaa ttatgtaccg atcacaggcc cttctcggtt
    10861 aaatatgaca gatatacggc aatacactta cttcctttgg aacatactat atatgatttt
    10921 tcgcctatgg ttatgagaaa taatgagatg gtaactcttt acgataatga agtattgcga
    10981 aaatattttt ccacattcat tttacaggcg gatgaggata tgcagatgat aagtaatctc
    11041 ttaaaatcaa caaatgtcta tccgacagat gacttgattg gtgcaatgac atatgatgat
    11101 atcggttcca taacaacgga cagtaaaaca catgatatat tgaccatgat taaaccggca
    11161 tcgagtatgg aatttgttcg ttgtgtaggc cttatatacg gacaaggttg ctggtatgat
    11221 aatgcggaaa gactgattca tgaacgactt acggatatat atgacgtcat atgttttaaa
    11281 cacgaacttg atgacgagaa aaaagatgat gatatgcata aggaatctaa aagaaagatt
    11341 aaatactgta ttacatacga ggaagcattg attcattcca aaaatatgat gatattaaaa
    11401 tggtatcatg ataattatcc aaagcaattt cagtttgaat atataaacag gtatagggac
    11461 ttagatacac cggaaaatca ttttataatt aaaaaagccg atatatccga ttcattaaaa
    11521 agcaggtatc tgcatgatga agaattaact gcaattaccc aaccgttaga tgtaataaca
    11581 tatttagacg ataaaatgat agataatatt ataattaaca gaccgtattc cagtgtggat
    11641 gattttgctt taagaaatcg aatagattcc gagttattgg aaaagcttaa aaccgacggt
    11701 tatctggaca gtctggataa aacggatcaa atcttaatca ctgatatatg ttaggtatat
    11761 caggcatgaa aactggaagt tgacaaagaa aatcatatat gctaaattaa gatagtacaa
    11821 aagagtggct cgccactctt taatttatga tgaggacaaa atgagtaaaa gtatagcaaa
    11881 acaggcgttg gaaatagcag ccggacatgc gaaaaagatg aatattgaag tgttggatgc
    11941 ggaatatgtt aaggaaggta atgagagata tctcagagtg tatttgtata aacccgaagg
    12001 tatttccgtt gatgattgtg agtttgtgca caaagctatt gataaggaaa ttgatgatct
    12061 ggatatcaga gaatcatatt atctggaagt gagttcgccg ggatatgata agaatttacg
    12121 tacggagaga gagtttgaga tatatatgaa cagtgaagtg gaagtttctt tatataaagc
    12181 tatggataat caaaagaaat ttacgggaac tttgaaagat agagattcgg aaaacacgac
    12241 tcttttaatt aatgattccc tgaagatttt tgataataag aatattgcaa agataacccg
    12301 agcatttaag ttataggagg aatgaaatga aaagagagtc tgtatcggat tcaagacaaa
    12361 acactgattt aattgatgcg ataaagcaaa tagaaagaga aagaggtata gattccgaaa
    12421 ctttgataga agctatcgaa gcggcattgg catcggcata taagaaacat acaaaaacgg
    12481 aacagaatat cgaagtgagt cttgatgtga acaccggaca ggtgaaactg tttaaaataa
    12541 agaatatcgt agacgagatt gaagatgaag aaaatgatat gctgattgat gaagcaagac
    12601 aatatgcgga tgtggaagta ggttattgcc tgaaatttga agaagaaatg gtaccgagag
    12661 atttcggtcg tattgccgca caaaccgcaa aacaggtcat tttacagaga ataaaagatg
    12721 ccgagagaat taaaatactg gatatatatg agaataagct taaggagatt gtttacggtg
    12781 tcgtaagaag agtggaaaac gatacggtgt atcttgattt aggtaattcc gaagcgatta
    12841 tgcccaagaa ggaacaggta cccagtgaac gttacggagt taatttcaag ttcaaagctt
    12901 ttgtttatga tgttaagaaa accagcaaag aacctaaaat atttgtttcc agaacacacc
    12961 ccgaccttat aaagcgcctg tttgaaatgg aggtaccgga aattcagagc ggaatagtgg
    13021 aaattaagga tatagcacgt gaaccgggtg caaggacaaa aatagcggtt tactcaaatg
    13081 acccgaatgt tgatgccacc ggtgcatgca taggacataa aggaataagg gtttccaatg
    13141 tagtagagga attaagaggc gagaagatag atatcataga atggagtccc aatccggctc
    13201 aatacatatc atctgcatta agtcctgcgg atgtttccag agtggatgtt attgaagttg
    13261 taaatgccga aggggagagt gagataaaag caagagtcac cgttccggat aatcaattat
    13321 cgcttgctat aggtaaagag ggacagaatg ccagactggc cgcaaaactt accaactgga
    13381 aaattgatat cattcccgaa acggaattaa gaagaatcat tgaagagcaa ctcttatcgg
    13441 atgatttact tgccaaagac acaaaaccgg taagcgagtt ggatgctttg tttgatcatc
    13501 ccgccgaaaa ggagaaagat gaagagtaaa aagattccca tgaggatgtg cttatcatgc
    13561 agaataatga aacctaagaa agaattgatt cgtattgtgc ttacggaaga aaataaagca
    13621 actatggatt tgacaggtaa gaagaacgga cgaggagcat atatttgcga tgatttacaa
    13681 tgtctgaaca aagcaaagaa atccaaaagc attgaaagaa cattttccat agagcatgat
    13741 gatactatat acaacgagat ggaggaatac ataaatagaa aagaataata ttctgaaaat
    13801 gataggattg gcaataaaag ccggagcagc ggtaaccggt acatccggtt gcataacaca
    13861 aataaagaag aaaagaataa tgcttgtttt tatggctcat gattgttcgg ataatacaag
    13921 ggagaagata ttaagagcaa taaaagaaaa aaatatagag atatcggaga tgttttccaa
    13981 agaacaatta ggtagtatta taaacaaaga agaaatatca gtgataggaa taagcgatgt
    14041 aaattttgca aaaggtataa gcgacaagat aaccaaggag aactaaatgg ggaaaagagt
    14101 aaacgagtta gcaaaagaaa tagacaagaa accgagtgaa gttctaagaa aattagagca
    14161 gatgggtatc gaatctaatg tcaatactgt tttaacagat gaacaggtgc agatgattta
    14221 tgatatttac agaataaaga atcctgcaac cggaaataag gtcagagcaa ccgtaataac
    14281 ttcgtctgat gccgcatcac ctgtcaaaac cgttgtagtt aaagctcccg ttaagccgaa
    14341 aacaaaaacc gagcataaag acgaaagtga taaaccgatg gttgttgttg taaaaactac
    14401 caagggcggc gaaaccaaat ccacaatcag acacataaag caagaaccgc aaaaaagccc
    14461 ggaaacaaag aaagaaacac ctcaaaaacc ccaggaaaag aaagtagtcg tagttaaaac
    14521 acctgaagtt actgttcccc aaaaagagag tgccggacag acggcgaaac cgttaaaagc
    14581 tgaagaacag gaaattaaga agcctcaagt ggcggcacct caaaaaaccg aacaaaagac
    14641 atcggataag gaagttataa ctaaaaagga agacaaaaaa gaaaagacct atatagaaca
    14701 aaaagcatat aaagacaaaa catttattcc gaaggaaaga accgataagc cctatccgcc
    14761 gagggatacc agaaccgata aaggctcata tcaacaaaga gacaacagga cagacagacc
    14821 ttatgcacag aaaccgggcg ataaaaagag cggatataca cgtaccgatt tacatgacgg
    14881 taaacagaaa cctgcagcca ggaggacgat tgacagggat agcgatgacg atacttctac
    14941 aagaattgaa aagccgaggg taataaagaa agaatacata aaaaaagatc aggaaaaggt
    15001 actgaataaa gaaacaaaga aggaaacagc cgttgcaaga tctatggctt caaaacaaac
    15061 taaatatgaa aagagagaaa tattaactcc gaaaggtgca atagatactt tcatgcttac
    15121 ggatgatgaa ctgttcagta cattttacag agatggcgga acaagaagga aaagaaaaaa
    15181 aagagatgca agtcaaccca aaattgaagt actcaagttt gtaaaacttc ctccggaaat
    15241 gactgttaag gaatttgcgg aaaccattaa gaaaacaggt tccgatgtat tatccaaatt
    15301 gctggatatg ggatcaggta ttatgagtat aaatgaaagt atcgattttg atacggctgc
    15361 tttaatagcc gatgagttcg gtatcacatg tgaaatggaa aacattatta cagaagaaga
    15421 tatcttattt gatgacgata aagaatgtga tccgaaagat cttgtagaga gacctcccgt
    15481 tgtagttgta atgggacatg ttgaccacgg aaaaacgtca ttattggatt atattaaaaa
    15541 ttccaatgtt gcagctaccg aagccggaag aattactcag catataggcg cttacagagt
    15601 taaaataggt gaaaggtata ttacattcct ggatactccc ggtcatgaag catttaccgc
    15661 tatgagaaaa agaggagcgg atgtaacaga tatagcaata attactgtag cagccgatga
    15721 cggaataatg ccgcagacaa ttgaggctat aaatcatgca aaagccgcaa atgtcaatat
    15781 tattattgct ataaacaaaa tcgatctgcc taccgcaaat cccgataggg taatgcagca
    15841 acttatggag cataatcttg tcgcaacaaa atacggcggc gatatcgaat gtattccggt
    15901 atccgcaaaa accggtgaag gtatagatac acttttggat acaatactct tatccgccga
    15961 tatactggag ttaaaggcca atcctaaaag acaggcaaaa ggaattgtac tggaatcacg
    16021 tgtggaaaga gcaggaactg tgacaacact tcttgttcaa agaggtactc tcaaaaagac
    16081 cgactatatt atctccggaa catcggtggg aaatataagg gcacttacag atgacagagg
    16141 aaatagcatt gattctgcag gtccttcaat gcctgtagaa atattaggtc tggatactcc
    16201 tcctgaagca ggtgagatat tctatgccgt agatgatgag agaacggcaa agtcattagc
    16261 agagagaaga aagtacgaac gtcagatggc taagacagct cataagctta ctactttaga
    16321 cgatttgtat gaacagatta aggccggaaa tattaaagat cttaatctta ttgtcaaagc
    16381 cgatgttcag ggttctgtag aagcattaac cgattcattt gtaaaacttt ccggtgacaa
    16441 ggtaaaagtg catattattc atgcagctgt aggaactatt acggagaccg atgtagctct
    16501 tgcagaagta acaaattcca tcattatagg ctttaatgtc agacctttag gtaatgttac
    16561 ggatctggca aaagaagctt ctgtggatat aagactttac agtatcatat atgatgcaat
    16621 cgaagatgtt aagaaggcaa tggcaggtat gttggaaccg ttaacaaaag aggttgtata
    16681 cggtcatgca caggtaagag aaacctttaa gataaccgga gtgggaacgg tggcgggatg
    16741 catggttctg gacggtaaaa tactcaggaa caccaatgta cgactggtca gagacgggat
    16801 tacgatattt gacggagcgc tcagttcact caagaggttt aaagacgatg ttaaggaagt
    16861 agctgcaggt tatgaatgcg gtatgtccat tgaaaaattc aatgatataa aaattgatga
    16921 tataatcgaa tgctacaaga ttgaggtggt caagaagtga agacaagaag agtggaacaa
    16981 ctgaacagtg aatttaagag agaaatatct tccattcttg ccgacgagat aaaagatccg
    17041 agacttacag ctattgtatc cgttttgaat gttaatgtta caagagatct ttcttatgcc
    17101 gatatacatg taagcatgat cggaacagag gagcagaaaa aagaaagttt tgaagcaatt
    17161 ttaagttcgg caggatatat acgaagattg ctgggacaaa gaatgaagat aagatatatt
    17221 cctcagttaa gattcaaata caacgattat attgaagagg gaataaggat aagtaaactg
    17281 attgatgaag ttatcaaaga tagcaaagca actgaataaa gatattaaga aatatgacag
    17341 tttcttaata ttgcctcata taatggctga cggagataca ataggctgct gtattgccat
    17401 ggccagatat attacttcaa gaggtaaaaa agcattaatg ttgttggaag aaaaaatccc
    17461 gtatatatac agttttatgg aagattcctg tgacatggaa gtgtatgttc ccgataagga
    17521 gtataacttt gatgtttgca ttgtattgga tacagcggat ataaacagat taggcagacg
    17581 aaaaagtttt ctgaaaaaca aggtatcata taatatagac catcataaaa ccaacgacgg
    17641 ttatgctatg tataatttta taaatgccga tgtttcttca gtaggcgaga ttatatatga
    17701 tctttttact aagatgaaag cttctattga ccatgatatc gctcaggcaa tatatgttgc
    17761 aattgccacc gatacaggcg gattccgata tcagaacacc aataaaaact gttttattat
    17821 atcctcccga cttatggatt acgatattaa tgtggaaaag atatccgaat gggtgtttga
    17881 acgtacaagc atgaataaac tgtacctgtt taagaatacc ataaaaaaca taaaactgta
    17941 ttttgataac agagtggcaa tatccgtttt aagacgtgaa gaatatatgc atcttgatgt
    18001 tgcggatgaa gactttgagg gacttgttaa tgttgtcaag aatatagagg gtgtggaagt
    18061 tggggtgttc ttaagagagc gaaacagtga tagagaagtc aaagggagct tacgatccaa
    18121 tacggatgaa attgatgtat ccgttatagc taaaaccttc ggaggaggag gacatgcaag
    18181 agcagcaggt ctcagttgta atatgacctt agaccaggtt tatgacggaa tgatagaaaa
    18241 attgaatgaa gtgtacggat gtacggaata atcaatcttt acaaagaaaa gggactgact
    18301 tcgtttaagc agttaaatat gcttaaaagt gttttcccgg gtattaaaat aggtcatacc
    18361 ggtacattgg atcccattgc ggaaggagta cttgttgtct gtataggcaa agcaaccagg
    18421 atagccgatt atatacaagc acaaaaaaag gtataccggg cggaaatgtt tcttggtgta
    18481 tcaacggatt cttatgatat tacaggtaag atattaagca ttaattcgtc ggatgatatt
    18541 tctgagacgg caatactgaa tacaattaaa ttattcatcg gaaagcaatg gcagaaacct
    18601 cctgtctttt ccgccaaaaa ggttaaaggg aaaaaattgt atcaatacgc ggtaaatcaa
    18661 caacctgtgg agataaaagc atcatatatc gagattcaaa atatcagcaa cattttaata
    18721 aacgattata tttacgagaa tacacctgtg aaaaagattt cttttactgt ggaatgttca
    18781 agaggaacgt atataagaag tctatgccat gatataggtg aaatgctgaa aaaaggcggt
    18841 acaatgtcgg cattgacaag actgcaggtg ggaaatttca aaattaccga cagctataca
    18901 gttgatgaaa tattaaaaaa ggcatctgaa aataatttgc agactatctt tacaccgatt
    18961 gaaaacgcaa ttgatattaa tgatatttat ttgactgccg atgaaattaa gaagtatatt
    19021 aacggtgtta aaattgagaa gaaacttaaa aagggagaat atataataaa ggatgtaaac
    19081 gatacggtta taggaatcgg atatattgat gagaataata tattaaaagg gaaaatatta
    19141 ttaaatgaag attattaaaa gtgaagacaa ttatatattt gaaaagagca atgtcatagc
    19201 gttgggtaac tttgacggtc ttcatatagc acatcgaaaa cttatagaaa cggcggtaag
    19261 tattgcaaga aagaacaatc ttttatcact tgtctatgtc tttgacatac atcctatgac
    19321 gatagtggga aatacaaaac aacgacttat aaccaatctt gagcaaaaac tggaactgat
    19381 aagcggtctg ggtgtggaca ttatatatat tcagaaattc gattatgaat tttccaaaat
    19441 gcaacctgaa cattttataa ccgatatatt aaaaaaacga ttatcctgtg cttatgccgt
    19501 ttccggctac gactataaat tcggatataa ggctgaaggc gatacggata caattaagaa
    19561 tatcggcagt aagcatgata taaaagtaaa aatcctggaa agaattgatt atctgggtca
    19621 gccggtaagt gcatcaagaa taagggaact tttggaaatc ggtgatatac aatatgtaaa
    19681 taatttattg ggaagagagt accaaatcag aggaacggtg gagaaaggca aactgatagg
    19741 ctcgaaaatc ggttatccta ccgcgaatat aatactgaaa gatgaaatgc agataccggc
    19801 attcggagtt tatcagacag ttacaacagt tgacggtatc ggatacgaca gtataaccaa
    19861 tataggtaat aaccctactg ttaataaaga ggatattgat aaaataacat gcgaaaccca
    19921 tataatagac aaattcgggc tgaaattata cggtaagaaa atttatattg attttataaa
    19981 aatgataaga aaagagcaga aatatcccga tattgaggca cttaagaaag caatagcccg
    20041 agatatttta cttgttaaat catactataa gtagtatact ctaaaataca tactttatgt
    20101 atttaagtac ggaaatgagg tgtaagaatg aaaagtataa tagcttttcc caatctgttc
    20161 ggaggtattg agttatcggt tagcagaatt gctttttcca taggcaaatt tgaagtttac
    20221 tggtacggat tgataatagc aattgctgtt tgtcttgcag ttgtgctggt tttaaaaaac
    20281 gcaaagaaat acggtataaa tgaagatgat ttattgaata tatgtcttat aggtattatt
    20341 gtaggagtaa tatgctcaag actgctttat gtactgggac gtttggacag agattggaca
    20401 tttgcagata tattaaatgt acgtgacggc ggtatgtcca tttacggagc attaataggc
    20461 ggatttatag ctacggcaat atattgtaag atcagaaaaa tgaattttct gaaactggcc
    20521 gatcttgtgg ttccctatat tgctttagca caggcaatag gcagatgggg taacttcatc
    20581 aataaagaag ctttcggtgt acctgttaat ccaaacagta ttttcagtac tctggctatg
    20641 acaagtatga aaataggagg caatgtggcc ggctcaatag aggaagaatt attaaaatta
    20701 ggatttgttg atacctcatt ggtagcaacg gtaaacggta atacatttga ttatatagga
    20761 gttcatccga cattctttta cgagtcggtc ttaaatctca ttttgttttt agttcttata
    20821 ttggtggcaa ggaacggaaa gtacttcaac ggtcgttcat tatgcatata tatggcttca
    20881 tacggaatat acaggttttt cattgaggga ataaggatag accctctata tctgtttgat
    20941 acggatatta agctctcacg cttacttgca ttgataagtg ccatattctt cggaacatta
    21001 ctgattatta tatcaatatt acgtgctaaa gataaaaagt tgtttataaa agctttactt
    21061 gatgaggaag aaacagatat aggtaaatcc gactataagg atctccttgt agataaagac
    21121 acatatgtaa gtgagaataa tactgaggaa acaacggaga aagaagaatc ggaaaaagat
    21181 attgtatccg aagaagaaca atcggttgac aaataataag ttaaagttat aataaaacaa
    21241 gtcggggtgt ggctcagttt ggtagagtac ttgaatgggg ttcaaggggt cgcaagttcg
    21301 aatcttgtca ctccgaccag acaatcgatt atcttcatga taatcgattg ttatttggag
    21361 gttaaacagg aataatgcaa tacaacgttg aagaatttaa aaaactggca atatctatgg
    21421 gtttttccaa tgccggacaa cttaatatgg atgcattggt tttcatgcct gaagttcgtc
    21481 agatgtgcaa ttccgactca tgcacaagtt ataataagaa ttggatgtgt ccgccggcaa
    21541 taggaacact tgaacaatcc gccgttaaag taagagaata cagcttcggt atgattgttc
    21601 agacaaccgc atttatgcaa gacgagtttg attatgaaac cattcgggat acacagaaaa
    21661 aacataaaaa taactttttt gaactggtaa cattactcag aaataaatat tcatcggttc
    21721 ttcctatggg agcgggtaca tgtacgatat gcaataaatg tacttatccc gataagccgt
    21781 gcagatttcc cgataaggcc ataggttcca tggaatcata cgggttatgg gtcagtaaag
    21841 tatgtgagat gtcatcaatt ccttactatt acggaaaaaa tacccttacg tttacaagtt
    21901 gttttctgct taaatagata tatgcaacag ttgaaaatat ttaaaaaaca catacaaaac
    21961 aggtaaaatg attatttata aaataaatat atgagtaatt gaaaaatttc cctttatttt
    22021 tacataaagt gataatataa taggagaaaa gtcattagac ttacatatta gtgttaaata
    22081 caaaagaaag tctggtatgc aaatgataag cgaaatcgaa cttttggaga ataataagat
    22141 atatattgcg ggaagcattg cttctgctcc cgtattcaat catcagatat acggagagtc
    22201 tttttataac ataatagtag aagttcaaag actgagtaaa cagaccgata tggtacctgt
    22261 tttgatttcc agtcatctac tggacattga tttattaaaa ccggacacat tgattgagat
    22321 taacggtcag gtccgttcat acaataatat tgataaaaac gaaaagaaga gactggtagt
    22381 caatgtattt gcaagggaag caaggatttt gcagacactg gaaggcatag aaagttttaa
    22441 tagtgttttt tttgattcct ttatatgtaa agagcccaat ttcaggatta cgcctttcgg
    22501 cagggaaata tgtgacctgc ttatagctgt aaacagagca tacaaaaaaa gcgactatat
    22561 accctgtata tgctggggaa gaaatgccag gtactgtgag aatatgaaag taggtacccg
    22621 cttaagaata tggggaagat ttcaaagcag agaatatgaa aagaaagatc attacggaaa
    22681 tatagtaacc aaaaccgcct acgaggtgtc ggttataaaa ctggaaaaag tagaggagaa
    22741 caaatgaaga taaatataga cgattgtata cgtttttcag acggaaaaaa attcgaaagt
    22801 atgctgagaa aggcaaaaaa agcggacaga ttattaaaaa gcggaaaagg tgccggctcg
    22861 gattatttag ggtggctgga tttacctaaa agaataaaaa aagaagatat aaagcttatc
    22921 aataaagccg ctgcaaagat tcaacagcag tcggatgtac ttgttgttat aggtataggc
    22981 ggctcctatt taggagcaag agctgcaatt gaggctctta ccgatgtcag aaatattaaa
    23041 ggcacggaag tcatatttgt cggaaataat ataagcggta aatatgttga gtctgttttg
    23101 gagtatctga aagacaaaga ctattctctg aatgttataa gcaaatcggg aacaaccgta
    23161 gaacctgcta tagccttcag aatattcaaa gaagctttat atgaaaaata taaagaaaaa
    23221 gctaaagaca gaatctatat tacaaccgac cctgttaaag gtgctttacg gtcgatagtg
    23281 gaaaaagaag gatatacaag ttttgaaata cctgccgata taggcggaag atacagtata
    23341 cttacacctt gcggtttact tcccattgca gtagccggta tagatataga gcagatgatt
    23401 tcaggtgcca aagatgcatt aaagaattgc acagagatgt ccgtaacata tgcggcagcc
    23461 agaaacttga tgtacaagag caaaaagacc atagagatta tggttaatta cgacccttcc
    23521 atgcactttt ttaccgagtg gtggaaacag ctttacggag aatcggaggg aaaagaaaat
    23581 aaaggtattt tcccggcagg cgtagataat accacagacc ttcattcaat ggggcaatat
    23641 atccaggagg gaataagaaa cattttcgaa actgttgtat acataaaaga cgatatgtcc
    23701 acaataaccg ttccagatat atcagataac tccgacggtc tggactatat taagaataaa
    23761 agccttaaat atgtaaatga aatggctatg agaggaacca aatccgctca tttggaaggg
    23821 gatgttcccg ttattcttat tgaattggaa aaaatggatg catttacatt cggtaaactt
    23881 atatatttct ttgaaagagc atgcggaata agcggatata tcttaggtgt taatcctttt
    23941 gatcaacccg gtgtggaagc ctataagaag agtatgttca aattattggg gaaaccggga
    24001 tattgattat gacggaaaat gaaaaatcgg taaaaaaggc aaagaagacc aatacacgaa
    24061 aattgagcag taaagttatt ttcatactga gtttagtaac gttgtttgtt atttgtatta
    24121 ttatctcact cataattttc tttaattcca tgcatacatc aatttatgaa actcccagtc
    24181 ccactccgag taaggttgcc gagaccatta ctttgcctcc cagtgagaca acttcaacgg
    24241 aacctgtcga aacgcaagtt acggaaacac cggcaccgac gcctatacct atgtataaac
    24301 cgcaggatga catagagttt cttcttttgt tcggattggc cgagtatgat ttagcggata
    24361 ccatatggat aggaattgtc aataaaactg acaataaaat agatttttta agtataccca
    24421 gggatactta tctaaggtat aaggacagaa ataatcccga tcattggaag ataaattcct
    24481 attacaacaa ctatgtgaat gaccaatggg taaagacgag cgactccgca tcatccatgt
    24541 atttgatggg tgcatgtgaa gcattgctga atatagatat ggactattat gtaagggtat
    24601 catatgacac aatagaaaaa attgtggatt ccatgggcgg tattgagtat gatgttccgt
    24661 tcagaatgga atattatgat ttaacacccg gtcatgaact tataatcgag attgacgaag
    24721 gattacaagt attgaacggc gagaatgttg ttaagttttt acgattccgt cagggaaccg
    24781 gatatgatta catttccgat atagcaagaa ttaacagaca acaggatatg gtaaaaacca
    24841 taattaaaaa ggctttcaca ttatccaata tatccgccgt tattgaggtt gcaaaaaata
    24901 atataaggac caatttcgga aaagatcagc tgacttcctt tataacattc tcgcttagtc
    24961 ttaacagtga aaagatttct ttccatactc tgagaggtta tgaagatatg gtcaatactc
    25021 aagtaaaaga ttcggacggc aatgatatta ttcgcagttt ctatatatat tccgatgagg
    25081 aagtaagaaa gcaactgata tctatatgtg aatagctttc aatctaagta cccttactat
    25141 tgacaaataa ccgcttttga tattaaatta atcaggataa aggatatatg caaaacaaaa
    25201 aggcgtttta aaagcatgta tcaagattta atcgtctttt acaatttatc ggtaagagac
    25261 tttttttaaa aggaggccca atgaagatat ccataccgaa gggaactaaa gatatactgc
    25321 ccggagagat agagaaatat aaatatgttc agactgcatt tgcagatatg ctggaaaatt
    25381 tcggatattc acagataaga tttcctgttt tcgaatacac tgagttattt caaagagggg
    25441 taggcgatac aaccgatata gtacaaaaag agatgtatac ttttaatgat aaaggcggca
    25501 gatccgttac attgagacct gaaggtacgg catcggtagt cagaagctat atagaaaacg
    25561 gcatgtcatc caatacccaa cctgtaaagc tatactatga tattacggct tacagatatg
    25621 aaaatgtgca aaaaggcaga tacagagaat ttaatcaatt aggagcggaa atattaggtt
    25681 ccgattcatg taatgcggat gtggaagtat tatttatgct gtactctttc tttaacggat
    25741 taggtattca gggattacat ctgaacataa acagtatcgg atgcaaaaca tgcagagcgg
    25801 aatataacag tatactcaaa gagtattttt ccgataaaac ggataacatg tgtgcggact
    25861 gtaaaagaag atatataacc aatccgatga ggttgttgga ttgtaaagta gataaatgta
    25921 ttgagattgc taaagatgct cccatgcttt tagataatct ttgtgataat tgcaaggaac
    25981 atttttataa tgttacggca ggactggatg aaattaatat accgtatatt atcaataaaa
    26041 ggattgtcag gggactggat tactatgtaa ggacggtatt tgagtttatt gcggaaggtt
    26101 taggtgcaca gaataccgtt tgtgcgggcg gaagatatga cggtcttgtt gaacagctgg
    26161 gcggtaccga tactcccgga ataggttttg ccatgggact ggagagactt gtggaaacca
    26221 tgaagaattc cggctttaag tttccgactg caaaaacccc tgtaattttt atcggttaca
    26281 taggcgaaaa agcatttaaa gtagcaagaa ctattgcaaa tgaactgagg cttaaaggaa
    26341 tatattgcga gattgatata aatgacagaa gtttaaaggc tcaactgaaa tttgccgaca
    26401 aaaaggatgc tttatacagt gttgtattag gtgatgctga aatagataca aataaagcaa
    26461 ttattaagaa tatgaaaaca caggaaacaa aagaagtcag tattgattcc attgtaagaa
    26521 gagttgtgga aaatataata tgaggtataa tatggagaac ataaaaggct taacaagaac
    26581 cggatattgt gcggaatatg atataaaaga tatcggtaaa aaagtcgttc ttatgggatg
    26641 ggttaacaga tcaagaaact tgggcagcat tatattctgc gatatgagag acaggacagg
    26701 tcttattcag gttgtcttca gagaagaaaa aaataaaagt cttttcaata aggcgaccaa
    26761 tctcaaatcc gaatatgtaa tagccgtaac aggtattgta aacgagcgtg aagataagaa
    26821 tcctaatata aagaccggag atattgagat tgaagcagaa gaattaagaa tattgtcgga
    26881 aagtgccgtt cccgcaatag atattgccga aaatacggct acaaatgaat taatgagatt
    26941 aaaatatcgt tatctggatt taagaaaacc caagcagcag tactatctaa tcatgagaaa
    27001 taaagttgca aaatgcacca gagatttctt ttcggagaac ggatttattg aagtggaaac
    27061 accggttctg acaagaagca caccggaagg agcaagggac tatcttgttc cgagcagagt
    27121 aaatcaagga aaattctatg cattacctca atcgcctcaa cttttaaaac aactgcttat
    27181 gatttccgga ctggacagat actatcagat tgtaaaatgt ttccgtgatg aagatttaag
    27241 agcaaacaga cagccggaat ttacgcagat agatctggaa atgagtttcg tggatgtaga
    27301 tgatgtaatt gcagtcaatg aaaaattcat aaagaaacta ttcaaagaaa cactgaatgt
    27361 cgatgtaaat attccttttg caagaatgac ctatgaagaa gccatgaaca gattcggcag
    27421 tgataagccc gatttgagat acggcatgga attaaccgat atttcagaat tggtaaaaga
    27481 ttcggagttt aaagtatttt ccgatgcctg taaaaataac ggatcggtaa gaggaataaa
    27541 tgcaaaaggc tgtgcggatt tttccagaaa agatatagat aaattaacgg attttgttaa
    27601 agactacaga gcaaagggtt tggcatggtt gaaagtagga gtagataatg aaatcaccgg
    27661 gtccatagtt aaattctttg ataaggagca gttaacgtct atagttgatg cgtttaatgg
    27721 cgaagaaaac gatttgatac tgataatagc ggacagaaat gaaactgttt tcgattcgct
    27781 cggtgcactg agaacggaaa tagcaaagag aatgaatctt gcagatcacg aaagatggga
    27841 gtttctgtgg gttaccgagt ttcctttatt ggagtttgat gaagaaacaa acagatatta
    27901 tgcaaaacat catccgttca catcacccat ggatgaggat ataccgttat tgcacaccca
    27961 tccggaaaaa gtcagagcta aagcttatga tatagtcttg aacggtgaag agataggtgg
    28021 aggcagtata aggatacatg attcggtctt tcaggaggaa atgttggaag tgttaggatt
    28081 tacaaaagat aaagcacaag aatctttcgg ttttcttctg aatgcattaa aatacggggc
    28141 acctcctcac ggcggaatgg catacggttt ggacagactg gtgatgagca taaccaaaac
    28201 cgacaatata cgcgatgtta tagctttccc gaaaattcaa agcggtgcct gtccgttaac
    28261 cgatgcgcct aatatcgtag agaagaaaca gttggatgaa ttgggtatat gcttgaagga
    28321 aagaaaagat gaggtttaaa tgttacttaa aactattatc agtttttata atacttttaa
    28381 gtctgatatt tataagcagt tgcgacattg ttaagtttcc cgtatatgtt tacggtctta
    28441 tagacacgga gggtaactat cttgtgcaac cggagtttac cggaataaag ataggaaagg
    28501 aaggtctgta tcctataaga caaggtatta acgaatacta taaatgggga ttttgtgaca
    28561 agaacggtaa tatagtaata gaacctcaat acagagatgc aagcatgttc agcgaaggaa
    28621 tggcacctgt acaagtatat gaccaatcgg aattatggat atttatagat aaagagaaca
    28681 atcaagtatt tgagaagcaa ttcaaatatg ctttaccgtt ctcggaagga cttgcatgtg
    28741 ttcaatcgtc ggcaagagat tcaacataca acttatgggg atatatcgat aaaacagggg
    28801 aatatgtcat agaacctcaa tttcagattg ccgcttcctt ttccgaaggg cttgccaaaa
    28861 tacagacagg atccggatcg gcaggtttat gcggatatat agacaagacc ggcgaaatag
    28921 tgatagaacc tcagttttcc tatgcgggac ttttttcagg cggtttggct tcggtaaaac
    28981 tgagtatgga ccaggagaca aacgatgccg gatatataga cagacagggt aatatagtaa
    29041 tacctttaat atactataat acttccacat tcagtgacgg attggcaccc gtacttgcag
    29101 gagaaacctt caaggacgca ctgtacggct atattaacgt tgataatgag atggttataa
    29161 aacctaaata ctatatggca tattcctttt ccgaaggttt ggcggccgtt aaaaccgatt
    29221 attatgagaa ctcgggatgg atgtatatag acactgaagg taagcttgtg attaaaaatg
    29281 actacagttc ggcatcatcc tttatagacg gtgtagcacc ggtcggaatg gtggaataca
    29341 gatgatgttt aataaccaat gggatctttc aaatatagtt gatataagca acaccaattt
    29401 agcgcaatca attttaaagc gtcgcaatat agaagacctc aatacatatc tttatcctaa
    29461 actgagtgat ttacatgatc cgtttctgct taaagatatg gataaagccg ttgaacgtat
    29521 tatgcgtgca attgaagata aagaattaat tctgatatac ggtgactatg ataccgacgg
    29581 tatcacggca acggctgtaa tatatagatt tctaaaaaac ttggacgcag aagcaacata
    29641 ctacatacct aaaagaagcg aaggatacgg attaataacg gaagtattgg agaatatact
    29701 gaatgaagat atcggattga taataacggt tgattgcggt attccgtctg ttaaagaagt
    29761 ggactttata aatgaaatag gaatagactg tatcatttcc gatcatcata cagtacagga
    29821 agaaattccc gatgcatatt gcattatatc tccgaaagtt tcaaaatctt atcctttcaa
    29881 tgaattaagc ggagtcggaa tagcattgaa gattgtgcag gctttgtcca tcaaactgaa
    29941 taacccgcag gaatatgcaa agtatattga tttggcggca ttaggtacaa ttgccgatca
    30001 ggtaccgtta attgacgaaa acaggaccat tgccaaaata ggcatggaca gtattccttt
    30061 tacacagaat gtcggtttga aagaactgat aagtcaatcc tatcccaatg gaatcaataa
    30121 agtaaactcg tctaatctta tgtttaatat aatacccaag attaatgcat ccggaagaat
    30181 ggagtcaccg gaatatgcaa ttaaactgct tatatcggat gattatgagg aatgcagaaa
    30241 attatcggaa aaattaattc aattaaacga agaaaggaaa atactccaga aagaaataac
    30301 cgattcggtt ctggacaaga tagaaagcaa cgaaagtata aaaaatgatt cggttattgt
    30361 tctttataat gaacaatggg agataggaat actgggaata gtagcttcta aaatagtgga
    30421 taaatataac agaccgtgta tattgctggg aggtaacgaa aaatacttcg gtgaggagag
    30481 aataataaaa ggtaccggac gttccgttga aggtttcaat ctgattgaag cacttattca
    30541 ctgtcattct tttttggata aagatgtaga aacatgcgga ggtcactatt tcgcagccgg
    30601 taatgaatta aaggaatcaa gtgtggataa atttcgcaat attattaatc aatatgcaaa
    30661 agataaggga tttacggttt ctgaacgaaa gttgctcagt cccgactata aagtcaacat
    30721 atctgatatt aatgcggata atgcacggat tctgtcctgt atggagcctt acggttcgga
    30781 aaatgaatta aatctcttct acatagaaga tatgatactg gcaaattggc gcctgatagg
    30841 caagacaaaa gatcatatga ctatgtcatt taaaaaagac ggcagatata tgaactgtat
    30901 ttctttcaac agtgcaaaat acagcaaatt attgcttaac ggattaagtt atgatttggt
    30961 attcaagatg tccttaaata cggtacggga ttcaaattca tttaccaagg acgtcagata
    31021 ttacgtaaat tgcgagattg cagatatgag aatcaaagat attcctttag ctattatgga
    31081 tgacttggtt aagtattcaa aatctgatgc aaatatagaa agagaggata tagcaaaagt
    31141 ctatagattg ataagaagca atac
//
LOCUS       DAOSOM010000024        30328 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_1529_length_30328_cov_6.457107, whole genome shotgun sequence.
ACCESSION   DAOSOM010000024 DAOSOM010000000
VERSION     DAOSOM010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 30328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30328
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1529_length_30328_cov_6.457107"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..1222)
                     /locus_tag="PLF14_06640"
     CDS             complement(89..1222)
                     /locus_tag="PLF14_06640"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT71001.1"
                     /translation="MNSRERLITVLKGEIPDHVPVSPDISQMIPIRRTGLPYWDIYLY
                     QRIPKWKAYIDSVKYFGFDSLMDGFANIQFEELGEFYNPWTEVIVHKDQLKIITQKYR
                     TEKNEKVWEPLVSVYHRDNPPSWDVHPHLVKLPLIPDRYEPVEDVSQWPTGEALLKWV
                     KEEMGNHGLVGVTCGTSIVLKNSEMVYDFYDNPDKYYKKRDELIEYYEKRLHKLMSLE
                     HKPDFIGTGGSGSLIFQTPEIFRELALPIVKRVAKLCKQYNIPSHVHSCGPQKEMVKM
                     LAEETELTVIDPLEIPPMGDCNLKELKKLYGDKIVLKGNLHTTEVMLRGSVQDVTDAC
                     KRAIDDAAEGGKFILSTGDQCGRDTPDENLFAMIETAKTYGRY"
     gene            1349..1591
                     /locus_tag="PLF14_06645"
     CDS             1349..1591
                     /locus_tag="PLF14_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008752838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOT71002.1"
                     /translation="MNINTDTIVSMTEANQNFSKVARLVDEKGMAVIMKNNAPRYLVM
                     EFKQLKEQQLASDEDALTLSKRFIDSNRKAYTELAK"
     gene            1716..2984
                     /locus_tag="PLF14_06650"
     CDS             1716..2984
                     /locus_tag="PLF14_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT71003.1"
                     /translation="MKKEPGKTVNLAFVGLGKRGLSLLNLVLVAMDDIKVTALCDVYQ
                     DRTDRAADLVEEKTKERPFTTLDYKELLDRKDIDAVIVPSSWETHFDICLDFMKAGKY
                     VASEVGGANSVEQCWDLVKTYEKTGTPAMILENCCYGKTELTVLNMIKQGVFGELIHA
                     QGGYQHDLRTEVATGVEKRHYRLDNYKHRNGDLYPTHELGPIAKWLSVNRGNRMTYLC
                     SMASKTAGITDWIRKNRGPDYDNANFKFALGDVVTTMIKCAHGETILLVHDTSLPRPY
                     SRGNRLQGTNAIWMEDKWAFCIDGVSPVNKWGEYRWESFDDYYEKYEHPLWTWYRQRG
                     LIGGHDGMDYLTLRAFLEAVKNGTDTPIDAYDMATWMSITCLSEQSVAMGGHPVPIPD
                     FTNGRWMHREPAIPGRYCLDEICGPEYFEI"
     gene            complement(2998..4134)
                     /locus_tag="PLF14_06655"
     CDS             complement(2998..4134)
                     /locus_tag="PLF14_06655"
                     /inference="COORDINATES: protein motif:HMM:NF024136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HOT71004.1"
                     /translation="MKRKKKKRSKTKNTNIIKAVTMLVAALCFAFLTAVLVTILKRVP
                     GFTNGIYTKWSVFAMHTLANFFSFVKFSVAEVLIYILSVIFLSSLVILIVNLIKKKHR
                     VTRIFKFITAWMLVFSVTAFLFYALWGGMYYSKGIGNYLPVNSEDIKERPVEELVNLN
                     KYLIEKTNRLAAEVPRNQQGYYTGYEFQRTAAFTAKEVSKITGKEEAPPKYVVSSDAW
                     SYTQTTGIFIFLTGEANINKNNLAIALPFTCAHELAHRNGITSEDEANFFAFYSLHNS
                     ANINLRYSAYAMTLMYTMNSLYSKDAAAHKELASTYCDLLKHDYIRYHEHWDRYEGKT
                     SEISDKVNDTYLKAQGQSDGIYSYGRVTDIMLAWYENDIVKNKK"
     gene            complement(4106..4273)
                     /locus_tag="PLF14_06660"
     CDS             complement(4106..4273)
                     /locus_tag="PLF14_06660"
                     /inference="COORDINATES: protein motif:HMM:NF024081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HOT71005.1"
                     /translation="MKPLDYILIAIVALIVLSIVLYLIKSRKKGGSHNKCSACPYSGQ
                     CSDEKKKEEEK"
     gene            complement(4277..6277)
                     /gene="feoB"
                     /locus_tag="PLF14_06665"
     CDS             complement(4277..6277)
                     /gene="feoB"
                     /locus_tag="PLF14_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004465524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOT71006.1"
                     /translation="MIIALAGNQNSGKTTLFNQLTGSNQRVGNFPGVTVDVKEGIYKA
                     DKSIKIIDLPGIYSLSPYSPEEILTRDYLIKQRPDGIINIIDGTSLERGLYLSLQLLL
                     LNIPMVIAINMMDEVYANGIVIKTDILSEYLKVPIIPVCANKNEGITDLMQALKDLVD
                     NRCTPPKQDFCTGAVHRTLHATAHILEDHAQRANVPASFAAVKIIEGDEPMMADLALG
                     ENEIEMIEHAITEMENELSLDRMAAIADMRYQYIEDVCKKAVTRPKCDKHHDRTIKID
                     NVLTHKIFGIPIFLGIMLLIFWLTFGVVGSYLSDLLSGAIDSLAAIVSKGLTDFGINP
                     VVESLVIDGVFAGVGSVLSFIPTIIVLFFLLSLLEDSGYMARVAFIMDKLLRKIGLSG
                     KSIVPMLIGFGCTVPAIMATRTLTSDRDKKMTIFLTPFMSCSAKLPIYAVFTMAFFTE
                     HRALVMTAMYLIGILLAILVALISKKFLFKGNPIPFVMELPNYRLPTVKNTFLLLWEK
                     TKDFITRAFTIIFVASIVIWFLNKFDIRFNVVSDSKDSMLAAIGSLIAPVFTPLGFGN
                     WKASTALLTGFMAKEAVVSTLAVLLSTGIEKLSAPLATLFTPISAFSFLLFTLLYTPC
                     VAAVAAVRRELGTKYMFISIVFQTGFAYIVAMVFYQIAMLAG"
     gene            complement(6274..6504)
                     /locus_tag="PLF14_06670"
     CDS             complement(6274..6504)
                     /locus_tag="PLF14_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA domain-containing protein"
                     /protein_id="HOT71007.1"
                     /translation="MTLDKLGVGKSGRIIAVRGHGRLRHRLLDMGLTPRVIVHMQRKA
                     PMGDPVCIYLRGYELTLRLEDASMIDIEEISV"
     gene            6765..9041
                     /locus_tag="PLF14_06675"
     CDS             6765..9041
                     /locus_tag="PLF14_06675"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71008.1"
                     /translation="MAYKWCGHFDNEGKNYVITTPDTPRHWYNYLYNDEYITFTSQVG
                     FGEGFAQDKMGRRIPLVVNRNFFLCEDDYWSLAGLPIGYGYTDYSCTHTAGWSVIALK
                     YKDISSKVRIFVPNTGHMEIWSITLKNQSDRTRKLSLVAYAKTDIDAPYKPQGYNIAR
                     GSAFKDKNCVYGFFYTQFNTERNIPIYGYMSSSESIKGYDSRRTAFIGAYNDDQRPKA
                     LEETKGCTNSDCMVEKLCLALENSVTLKPGEEKTIHYVVGLAFGEEKIITPKDKWVQQ
                     QFAAMADKYEDRLRRIKIKTPWENFDNLINGWMVYATDMGSRWARVRHNGYRDMCQDT
                     DGLSVLNPELAWERIKRVLSYQYENGYAPRTIIDGELKDRKFSDNTVWLTYTVYSCIK
                     ELNKPELLHEQVPFNNGTSASVYEHIKRSVEFLFNFTGLYGLIRIWGGDWNDCINFAG
                     LEGKGVSVWLSIAWCAAAKQLCELAGMLGYEQDKKDYTEKYHIMRDRIDEHGWDGEYY
                     MYARTDEDITMGSHTQEEGKIFLNPQIWAVFAGLDKDDRHIKSMDMAEKLLQSPEGIL
                     LSAPAYTRQYHYIGSMAEKPGGVQDNGGVYLHPAAWKLAADSLLKRNDKVEENLKKML
                     PFDQTYYPTKGEPYCMFNCYYLDYHGYRGGTPGQSWRTGTNAWLLQSTVKYIFGLVPE
                     PEGLKLNPCPPPSWKECSITKLFRGCTYNIKYIQKGKGHNNRITRILMNGKETRFKDN
                     TIPPVEGATLDIVVYLEN"
     gene            complement(9073..9819)
                     /locus_tag="PLF14_06680"
     CDS             complement(9073..9819)
                     /locus_tag="PLF14_06680"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOT71009.1"
                     /translation="MSKYKCLIFDVDNTILQYDASAAKALEDLLLNHNVEYTPKIEEL
                     FRTVCYDLWKSYNMNLLDDPVMQEKYHEVYKIYGYKRFEVFLDKLNIGSLRKEHASDI
                     ADEYNEIFNNTHIFENHAIDVLKHCSKTHIVAAATNGIKEIQQSRLSPVKEYLSHLFI
                     SEEMGCVKPMKQFYNHMLETLNLSGNQCLFIGDLYTTDILGAINVQMDALWYINKERQ
                     SETAEWLESSNQYEITVPVIHSLLEIYRYI"
     gene            10009..12273
                     /locus_tag="PLF14_06685"
     CDS             10009..12273
                     /locus_tag="PLF14_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT71010.1"
                     /translation="MTEDITHILTNKPVPEEIAERLEKSADVQRLLFFIVGDLSKDSL
                     YSESVLAVTDKGMYCFDRTYDDGYKYVTHENIEKIEIKRLYGNAIMEISAEKEGTFDF
                     FRFTYSVANLCEAAADFSEQIKKGENIDRLMEMVTAVFEKQLSNCPRCGRTLVRPGAE
                     CLNCQSKGKIAKKLMKYVLPQKKLLIICLIFSIITTAMSLVPPYITKMMVDDIIPDNN
                     KKLLLTVVFVLFCTYVIQYSVGALRSYLLRVSGDRIVAGLRSDIYTKAQYLPMKFYDK
                     TSTGSVITRISSDTATLQSFTLRVTQEVIVQFFLLIGIIVIMFVMDRRLTLLSLIPVP
                     LVVIGSRIFGKKILPYYRRIWRKWSKVSSILTDTIPGVRVIKAFTNEKGAIDKFDKYN
                     EEWLKTDIKAARITTLFPQLVSFFVTCGSLLIWSVGGNLVMHPQSGLTLGLLVSFISY
                     TAMFYGPVNFFANFNDSLQAAVAAAERVFDIIDAEPEADFGKGNHPKKLEGKIQFKNV
                     NFSFDRTKMTLQDINIDIEPGDIVGIVGTTGSGKSTLINLIMRYYDNYDGKILMDGQD
                     IKDIDMEFYRSQIGYVQQEPMMFHDTIYNNIAYGNSDVQVEEVIHAAKIANAHNFIVK
                     LPDAYDTVLGERGTGLSGGERQRVSIARAVLRNPCIMIFDEATAAVDSETELLIQEAI
                     ERLIKGRTTLMIAHRLSTLRKANKILVVDEGRIIESGTPEELLAKKGKYYKLIEIQTM
                     SSKLQKAKEEENVE"
     gene            12285..12773
                     /locus_tag="PLF14_06690"
     CDS             12285..12773
                     /locus_tag="PLF14_06690"
                     /inference="COORDINATES: protein motif:HMM:NF020483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HOT71011.1"
                     /translation="MKVPYIQGDNAKIERADLTHVNVTMYDGRKFENVQPRRLFPISG
                     LRKYITLLDFEEKEVAIIRNLDTLMEDSKNAVDQCLNQYYLVPKIIRLYEIKEISGNI
                     NMHVLTDKGERKFEIWHRYNDIKIIHGTRMLFRDTADNRYEIEDIDKLDKVSRAKLAT
                     FI"
     gene            12797..13123
                     /locus_tag="PLF14_06695"
     CDS             12797..13123
                     /locus_tag="PLF14_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HOT71012.1"
                     /translation="MKLILAIVNNDDSAIAASALTEAGYFVTKLSTTGGFLMVGNTTL
                     LIGTESKGTEHVIEILSKYCKTRKQTVLSTASFGVGLSNESLPPEVTVGGAAIFVLNV
                     EKNIKI"
     gene            complement(13125..13550)
                     /locus_tag="PLF14_06700"
     CDS             complement(13125..13550)
                     /locus_tag="PLF14_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71013.1"
                     /translation="MRKKFNQMTDMEKSVRLKNHYIVHIAFYAFMFNLLPVAFMGSHI
                     EGFLVYMLILGFPAVIYIASIIFGLFNGFKWLYILFPAVLFVPAAFIYYDYSALLYAA
                     LYAAVSALGMFAGFILVKLFTKKPATSAGSEKVQDRKKK"
     gene            complement(13597..14151)
                     /locus_tag="PLF14_06705"
     CDS             complement(13597..14151)
                     /locus_tag="PLF14_06705"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOT71014.1"
                     /translation="MVATVETERLILREFRFDDVKDLYEYASNPNVGPNAGWTPHKNI
                     QESESVLNMFMRADNLWAIVYKENHKVIGSIGLHDDILRSQIKDLKTRCLGYALSYDY
                     WGMGIATEAVKAIQEYAFNVMKLDLLSVDHYPHNSRSMKVIEKCGFKREGTLRKARLS
                     YRNEPVDIVTYSLLKEEWKELNGR"
     gene            complement(14197..15804)
                     /locus_tag="PLF14_06710"
     CDS             complement(14197..15804)
                     /locus_tag="PLF14_06710"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOT71015.1"
                     /translation="MKVYVLQPSYPKSGDEKKNTVGFILQSLDSVGTDADLILLPEFA
                     NIPGASQYDEMIADYNRNTDILTKRVIATAKRTNSLVAVNMAHKTDTGFRNSTLLYNG
                     SGELIYRYDKQHLTYAEKELLKLDYSYRYRTDIPCFVDVGGLRLAFMTCYDTYFLEYI
                     EYIRSKKPDIILICSYQRSEEEDIILSQARIISSRCNAYVLRASYSMGNTAKGGHSLV
                     CDCDGTVLADMEQLTGVLRADIKVPKKAMKPNGHGQPLIPTDEFVTQGRTPHAYLATG
                     SFISKSDNEKPYPRICAHRGFSALCPENTMLAISAAVALGADEIELDVWPTKDRVMIA
                     MHDPHFSHDKSKKVWEYTFEEVMELDASMGMSPMIAGMKYDTFEDILKRFHHQTIMNL
                     HIKTKFIDKYSNIVFPYDEDDFKVIVDLIEKYDCADYIYIAGDEAVMETALKVAPHLK
                     RCCLDGQLDYTLVDKAIKYKCDKLQFFKPYFNDEMIKKAKDNGIICNIFWSDDPKEAS
                     GFLDRGIDTILTNNLHLVQQGVKHRLG"
     gene            complement(15807..16307)
                     /locus_tag="PLF14_06715"
     CDS             complement(15807..16307)
                     /locus_tag="PLF14_06715"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOT71016.1"
                     /translation="MQYHIRRAKEKDAEDILRLLGQIAQLHHEKRPDIFKSNASKYNN
                     KLILEKIKRPEEYIAVAVDETDKVAGYLFCVFDIVKNHTVQQNRKILYLDDLCVDEDK
                     RGIGIGSLLINHAYEYAKKEHCDAVELNAWEINERAVKFYADHGFTVKYRRMELIVDS
                     KNDLER"
     gene            complement(16497..17495)
                     /locus_tag="PLF14_06720"
     CDS             complement(16497..17495)
                     /locus_tag="PLF14_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOT71017.1"
                     /translation="MKTVAKLSADERRELFRNTADKMGLNDAIVEKDFWVCFMLDYLF
                     HRSPWKEHLSFKGGTSLSKAYNLINRFSEDIDLILDWRLLGYGVDEPWIVRSNTKQDL
                     FNKEANTRTEEFLNETVCPKMKSDLSQELNSATNIYIDKKDKQTILFAYPNLFENAST
                     LKVIRLEIGPLAAWTPARKVVIESYAAKHYSSVFCQKTTEVLTVLPERTFWEKATILH
                     HEANRPKNLPMPKRYSRHYYDLYRMADTPVKSTAFDSLELLTKVAEFKMKFYPRAWAK
                     YQEAKRGTLKLIPPSYRIPALSEDYQNMRNMLYGEIPTFNTIMNRLSELEKEINVI"
     gene            complement(17501..18106)
                     /locus_tag="PLF14_06725"
     CDS             complement(17501..18106)
                     /locus_tag="PLF14_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003094863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="HOT71018.1"
                     /translation="MSNGYADKIRERVLSATNGTVFTTSDFSDIADNNTIRQNMLRLV
                     NEGVLKRILGGVYEKPKYSKLLGEYVAVNPDAVAQALARSYHWTIAPCGNTALNMIGL
                     STQVPATWSYISDGPYRKYEWNSTCIEFKHRTNKEITGLSYMTALLIQAIKTLGKESI
                     TQDIIETLSEKLTRKEKDAALKEASRSADWIFRIIKQISEV"
     gene            18297..19115
                     /locus_tag="PLF14_06730"
     CDS             18297..19115
                     /locus_tag="PLF14_06730"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019911917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOT71019.1"
                     /translation="MIITPKYFKDKLPKALTMSYDDGPYEDYRLVELFNKYKIKGTFH
                     LNSRYMDRDDYPPQPEKIKMTDAKKLYEGHEISVHTVNHADLRDVNNLRFLHEVLDDK
                     KALEDIAGYPVRGMSYPYGYYNDEVVQRLRYLDMEYARTTKSHKSFYLPDDFLVWHPT
                     CHHNDATDLISAFDSPRWNMGMNLFYVWGHSYEFERWGDKWYIIEEFCKRISGREDIW
                     YATNIEIKDYVDAVRNLKFTVDTSMVYNPSCTDVWLDVDREPVKVAAGQLTKLK"
     gene            complement(19108..20277)
                     /locus_tag="PLF14_06735"
     CDS             complement(19108..20277)
                     /locus_tag="PLF14_06735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOT71020.1"
                     /translation="MNKLLIKLFVKDYKNTDDASVRSAYGMLASVSGLTVNILLFTGK
                     LFIGLLSKSIAVLADSFNNLSDAGSFLITLLASKLSKKPADKEHPFGHERIEYVAALF
                     MSFIIMNAGISLFVDSIKRITQPEPIIFEWAFVIALIVSMLLKAWVMLLNRDIGIRIN
                     SYPLKAAFRDSANDLVATSVTVISMLIAKYFNFSIDSYAGIGLSVFVFLSGLKIAKDT
                     LMPLLGSATDKKLYDKITGKVESYDGIIGTHDLIVHSYGPSNIMATIHAQVPNDGDMQ
                     KMHDIIDGIEKDVYRETGIHLVIHMDPQEVHDKRFFKYKSVVKGIVKKLDPDAAVHGF
                     NAKFDKNEINLVFDVTVAHSYTQEEEETLSENIKKELAKTNPQVKCDITVEHGFI"
     gene            complement(20313..21083)
                     /gene="nagB"
                     /locus_tag="PLF14_06740"
     CDS             complement(20313..21083)
                     /gene="nagB"
                     /locus_tag="PLF14_06740"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOT71021.1"
                     /translation="MKIIVKDNYSEMSKLAASVIADLVRKKPDCVLGLATGATPIGTY
                     HELARLHKEEGLDFSAVTTFNLDEYLGISMDLTLPYEMDQSYARFMHEELFKKINIKK
                     KNAHVPYGLATDPDKYCKEYEKMIADAGGLDLQLLGIGGDGHIGFNEPGSSFDSRTRV
                     EHLNQKSIDDNYEAFYKKAGVSKEEMPKKAITMGVATIMEAKSLLMIVSGIGKADIIQ
                     KALEGPVTTQITASAIQIFKRDVTVILDKPAASKLKKL"
     gene            complement(21163..21723)
                     /locus_tag="PLF14_06745"
     CDS             complement(21163..21723)
                     /locus_tag="PLF14_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71022.1"
                     /translation="MAFFDKLKKQATSAIEKALDPRHKTQKITFADLPETLEAFKALP
                     EAQLASPFETAALTVVAFCLYPVNKELCHQMLNYLRGPRPMSEMDKQFIRDRFLDKDY
                     VPRSYFDGATPANDYMPSKPYTITVTSNPRSFDDPDTVRLYINSGGADSPRLVSLRKA
                     KDGKWYLWEQYLLADIRKPESENPWA"
     gene            complement(21854..23035)
                     /locus_tag="PLF14_06750"
     CDS             complement(21854..23035)
                     /locus_tag="PLF14_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HOT71023.1"
                     /translation="MKKPVKNNVYWVGKTDWELKKFHGEELSTHHGSSYNSYLIMEEK
                     NVLIDTVWSPFEKEFVENLEKEIDLKKIDFIVCNHGEVDHSGALNKLMEKIPSTPIYC
                     SANAVKSLQGQYFKNWDLRPLKTGDTIDIGNGKQLVFVEMRMLHWPDSMACYLTKDNI
                     LFSNDAFGQHYACDHLFNDSADQCTLYAEAEKYYANILAPFSAILKRKLEEIQSLNLD
                     IDMIAPSHGVIWRDNPMQIVEKYRLWCDNYQENLITIVYDTMWNGTRALAEKIAEGIR
                     EKDESVNIRIFNLPKSDMNDVATQIFKSKLVLLGSPTVNNCALHSLAALTHYIKGLKL
                     KNKKAAAFGCYGWSGESVKILNEMLTDAGFEVINDGFKCLWNPDYETLQKAADFGKEI
                     A"
     gene            23259..23921
                     /locus_tag="PLF14_06755"
     CDS             23259..23921
                     /locus_tag="PLF14_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71024.1"
                     /translation="MGKSVYSLILNDEVIKKIDTLAYAKRTSRSGYINEVLASHVSYT
                     TPQQRIKDILNAARAFLEPYEKYAFVEMNSNSFMDVRTALSYRYRPTIRYCLEILGRD
                     KGPFLKLKAQVRTQSSSLISAIEDFFTIWQNVEKKLIPDTYDEVEMTSYENVCYTRFF
                     FLNDRMNIEEQRLGKAIAAYITTLDKALDIFMSNMDNTDYVISDIYAAYKEYYAKTGM
                     II"
     gene            23938..26544
                     /gene="clpB"
                     /locus_tag="PLF14_06760"
     CDS             23938..26544
                     /gene="clpB"
                     /locus_tag="PLF14_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004465455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOT71025.1"
                     /translation="MNIQKFTQKSVEALNRAQSLAIEYGNIQIEQEHLLYVLLSQPES
                     FIAQLMVSMNLNTGLLIQKAENLVSALPKVTGSGREPDKVYISKDTDLTLNEAEKQAK
                     IMKDEYVSVEHIFLAVIETANPRIRELFRETGITKDSFLRALASIRGNTKVTSDNPES
                     TYDVLKKYGQDLVELARNQKLDPVIGRDEEIRNVIRILSRKTKNNPVLIGEPGVGKTA
                     IAEGLALRIVRKDVPDNLKERKIFSLDMGALVAGAKYRGEFEERFKAVLQEVKNSEGR
                     IILFIDELHNIVGAGKTEGAMDAGNLLKPMLARGELHCIGATTLNEYRQYIEKDAALE
                     RRFQPVMVPEPSVEDTISILRGLKERYEVFHGVKIQDQALIAAATLSDRYISDRFLPD
                     KAIDLVDEACALIKTEIDSMPSELDDISRKIMQYEIEEAALKKESDKLSKEQLAKIRQ
                     ELAQLREEFNEMKAKWENEKASISKVQKIREEIEKVNAEIEKAEREYDLNKAAELKYG
                     KLPALTKTLAEQEEIAEQAEQSHTLLRDKVTEEEIALIVARWTGIPVSKLMQSEREKL
                     LGLEDILHKRVIGQDEAVTKVTECILRSRAGIQDPDRPIGSFLFLGPTGVGKTELAKA
                     LAEALFDDEKNMVRIDMSEYMEKFSVSRLIGAPPGYVGYEEGGQLTEAVRRRPYTVVL
                     FDEIEKAHPDVFNILLQVLDDGRITDSQGRTVDFKNTIIIMTSNLGTPYIMDGITEDN
                     EISDEARKMVDTLLKQKFRPEFLNRLDEIVLYKPLSEKEIMSIVDLMIEDLARRVENK
                     RLGLKVSKRAKEYIVDQGFDSIYGARPLKRYIQSHVETLIAKMILAHDLEPDTVLYVD
                     YDGDKLTVDTEG"
     gene            complement(26642..27631)
                     /gene="corA"
                     /locus_tag="PLF14_06765"
     CDS             complement(26642..27631)
                     /gene="corA"
                     /locus_tag="PLF14_06765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HOT71026.1"
                     /translation="MSENNLQKPNITVTEYNSDEIREYSTDSLETLKKDVKGVRWIDI
                     DNECGKVLCEQMSRLYNIHPVVLESIYEKDQSPKLDDYQDFLFAVATMIYKKEQALVA
                     EGISFLLGDNYVISIGDLDGDVFDKVRKELHTPKSRIRTSGPDYLLYRLMDSITDDYF
                     ATLYEIEERIYRLEEEIIKDPSDSLLAKIREIKSDNQLVAKSIIPFKESRSILINMPS
                     KFITSSVAPYMNDVYDHIKEAIGLSETCRVMISDLMQLFYASTSYKLNEIIKVLTVIS
                     TIFIPLTFIVGLYGMNFKHIPEFNIPWAYPVLLGVMLVITLLMIYYFKKKKWF"
     gene            complement(27628..28122)
                     /gene="mscL"
                     /locus_tag="PLF14_06770"
     CDS             complement(27628..28122)
                     /gene="mscL"
                     /locus_tag="PLF14_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOT71027.1"
                     /translation="MKKRVKRVKKEKTKLLEEFKKFITRGNVIDLAVGILIGSAFTKI
                     VSSLANDIIVPLVGLVMGKINLRELKWVLTYDAEGKALSSVNYGMFIQYIVDFLIMSA
                     CVFMLVRIFTALKKKSEELKEKAKKAEEAAAAATPPAPPAPPEPTKEELLLTEIRDIL
                     KAQK"
     gene            complement(28159..28842)
                     /locus_tag="PLF14_06775"
     CDS             complement(28159..28842)
                     /locus_tag="PLF14_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005367881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOT71028.1"
                     /translation="MKKIYTGKTKDVYCLEDGNYLLKFKDDVTGTDGVFDPGANTVGL
                     KMEGSGKAGLKLTKFFFEKLNSLGIPTHYISADIENRIMTVRPAKMFGKGVEVILRYR
                     AVGSFYRRYGMYCTEGQHLDGYVEVTLKDDNRNDPLITDEALQMLKIMTKEEYRILVE
                     LTRKTGSIVKEEMLKKGLELYDIKLEFGRVGTDNHIALIDEISGGNMRAYCKGKYVEP
                     LELEKIMLR"
     gene            29182..>30328
                     /locus_tag="PLF14_06780"
     CDS             29182..>30328
                     /locus_tag="PLF14_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009663765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT71029.1"
                     /translation="MKITELFSDVITEDMECEFKAVLNSDNPVKWAKSIVGFANGNGG
                     MLFVGVSEDREAFGISLDEIDKTKNLVAKINDRHIFPHVKLRYLMRNVDNAAEKFVLA
                     INISPSESIVRYREGDFNETVYIKGNGYSAPATPEEIITLSKRKFGVDNEISETLYVE
                     KQWTSYAELCREFRKDGSVPTVKELQNYEIVSKDGYAKTGFLMFRDGYDGDDTLICCR
                     LWRGKTKVGEVLDNGRFKGPLPDVFNETLRFIERNTKTGWHKTENGGREEIRSYPKKA
                     IREALVNAIAHRDYSIYGTQIDVDIYCDRIDIVSPGSWLLPKSYEEYPIGSIPSVRRN
                     TIISACLDVANLMERGGTGFQAIVDGYKDSSQDKQPCVLIYPGFLNLR"
BASE COUNT         8946 a         6028 c         6365 g         8989 t
ORIGIN      
        1 tcatagcggt tatcaatatt accgtcatta ccgcaattat ttttttctta cttaggtttt
       61 tcatttttta cccccgttat gttatttttc aatatctgcc gtaggtcttg gcagtttcta
      121 tcattgcaaa caggttttca tcaggcgtat ctctgccgca ctgatcacct gtagatagta
      181 taaatttacc accttccgcc gcatcgtcaa tagctctttt gcatgcgtcc gttacatcct
      241 gaaccgaacc tctgagcatt acttccgtgg tatgaaggtt gcccttaagc actattttgt
      301 ccccgtagag ttttttcaat tctttcagat tacagtcacc catgggcggt atctccagag
      361 gatctattac ggtcagttcg gtttcttccg ccagcatttt taccatctct ttctgcggtc
      421 cgcaggagtg aacatgcgac ggtatattgt actgcttaca cagttttgcc actctcttaa
      481 ctatgggcaa agccagttcc ctaaaaatct ccggtgtctg gaaaatcaac gatccggaac
      541 ctcccgtgcc tataaaatcc ggtttgtgtt ccaaagacat taatttatgc agacgcttct
      601 cataatattc tatcagttca tcacgctttt tgtaatactt atcgggatta tcataaaaat
      661 catacaccat ttcggaattt tttaacacta tggatgttcc gcaggtaact cccacaagtc
      721 cgtgattgcc catttcttct ttaacccact ttaacagagc ttctccggta ggccattgcg
      781 atacatcctc tacaggttca tacctgtcgg gaataagggg aagtttgaca agatgaggat
      841 gcacatccca ggaaggcggg ttgtctctgt ggtatacgct taccagaggt tcccatacct
      901 tctcgttttt ctcggtacgg tacttctgcg ttattatctt cagttgatct ttgtgtacta
      961 tgacttcggt ccacgggttg taaaattcgc ccagttcctc aaactgtata ttggcaaatc
     1021 cgtccatgag agaatcaaaa ccgaaatatt tgacactgtc gatatatgcc ttccatttgg
     1081 ggattctctg atataggtat atatcccagt aaggaagacc ggttcttcgt atcggaatca
     1141 tctgagagat atccggagac accggcacat ggtcgggtat ttctcctttt aatacggtta
     1201 taagtcgctc tctcgaattc atagttgcct catacatacg ccgttattat atactaattt
     1261 taacataaga taaataagat aaataattgc aattataggt tgtatttatc ctatatacaa
     1321 actataatta agacaggagg gactcatcat gaatattaat accgacacaa ttgtttctat
     1381 gaccgaagcc aatcagaact tttctaaagt ggcaagactc gtagatgaaa aaggcatggc
     1441 tgttatcatg aagaataatg ctcctcgcta tctggttatg gagtttaagc aacttaaaga
     1501 gcaacagctt gcttcggatg aggatgctct gactttatca aaacgcttca tcgacagcaa
     1561 tcgtaaagca tatacggagc ttgcaaaatg attggtctga tattgtattg tatatgctgt
     1621 tcaaagtgat ttaatccatg taaaattatg ctataatacc aaagagtgta ttaatatatt
     1681 acacgaaaga acatatataa atttcggagg tggatatgaa gaaggaaccg ggtaaaacag
     1741 tgaatcttgc ttttgtggga ttgggtaaaa ggggattgtc gttattgaac cttgttttgg
     1801 ttgcaatgga tgatataaag gtaacggcat tatgcgatgt ttatcaggac agaaccgaca
     1861 gagcagccga tttggttgag gaaaagacaa aagaaaggcc ttttacgaca cttgattaca
     1921 aggaactcct ggaccgcaaa gatatagatg cggtaattgt accctcctca tgggaaacac
     1981 attttgatat atgcttggat ttcatgaaag ccggcaagta tgtcgcttcg gaagtgggag
     2041 gagccaattc cgttgaacaa tgctgggatc tggttaaaac atatgagaaa accggtacac
     2101 ctgccatgat attggagaac tgctgttacg ggaaaaccga acttacggta ttgaatatga
     2161 taaaacaggg tgttttcgga gaacttattc atgcgcaggg aggctatcag catgatttac
     2221 gaaccgaagt ggctaccggt gtggaaaagc gtcattacag actggataat tacaagcacc
     2281 gtaacggcga tctttatccc acacatgagt tgggaccgat agccaaatgg ctttcggtaa
     2341 acagaggcaa cagaatgact tatctttgtt ccatggcttc aaaaacagcg ggaataactg
     2401 attggatcag aaaaaacaga gggccggatt atgacaatgc gaattttaaa tttgccctcg
     2461 gcgatgtcgt gaccaccatg ataaagtgtg cacacggcga aaccattctg cttgtgcacg
     2521 atacttcttt acccagaccg tattccaggg gcaacaggct tcagggtaca aacgccatat
     2581 ggatggaaga caagtgggcg ttctgtattg acggagtcag tccagttaat aaatggggcg
     2641 aataccgttg ggaaagtttc gacgactatt atgaaaagta tgagcatcct ctgtggacat
     2701 ggtacaggca aagaggtctc ataggcggac atgacggaat ggattatctt actttgagag
     2761 cttttctgga agccgttaag aacggtaccg acactcccat cgatgcatat gatatggcta
     2821 cctggatgag tataacctgc ttatcggagc aatcggtagc tatgggcggt catccggtac
     2881 ccattcccga tttcacaaac ggcagatgga tgcacagaga gcctgcaata ccgggcagat
     2941 attgtctgga tgagatctgc ggtcccgaat attttgagat ttagttataa cagttaatca
     3001 ttttttgttt ttaactatat cattttcata ccatgccagc attatatcgg ttacgcgtcc
     3061 gtaactgtaa attccgtctg actgaccctg agctttcaga taggtatcgt taaccttgtc
     3121 ggatatttcc gatgtctttc cttcatatct gtcccagtgc tcgtgatatc ttatatagtc
     3181 atgttttaat aaatcgcagt atgtcgatgc aagctcctta tgtgccgcag catctttgga
     3241 ataaagagaa ttcatggtat acatcaatgt cattgcataa gcgctgtaac gaagattaat
     3301 attggcggaa ttatgaagtg agtagaatgc gaagaaattg gcttcatctt ccgatgtgat
     3361 gccgtttctg tgcgcaagtt catgtgcaca ggtaaagggc agtgcaatag ccagattatt
     3421 tttatttatg tttgcctctc cggtaagaaa aatgaatatg ccggttgtct gggtatatga
     3481 ccatgcatcg gaagatacca catacttagg aggagcctct tctttaccgg ttattttact
     3541 tacctctttg gctgtgaaag cggcggttct ttggaattca taccccgtgt aatatccctg
     3601 ttggtttctg ggcacctcag ccgccagccg gttggttttt tcaatgagat atttattgag
     3661 gtttacaagt tcctctacag gtctttcctt tatatcttca ctattcaccg gtaagtagtt
     3721 gccgatgccc ttggaatagt acatgcctcc ccataaggca taaaagagaa aagccgttac
     3781 cgaaaacacc agcatccagg ctgtaataaa cttgaatatg cgggttactc tgtgtttttt
     3841 ctttataagg ttgacaatca gtattactaa agaagacaga aatattacag agaggatata
     3901 tataagtact tccgctacgg aaaacttcac gaaagaaaaa aagtttgcta aagtatgcat
     3961 tgcaaagaca gaccacttgg tatagatacc gtttgtgaaa ccgggaactc tctttaatat
     4021 tgtaaccaga accgctgtca aaaatgcaaa gcacaacgcc gctacaagca ttgtgacggc
     4081 ttttatgatg ttggtgtttt tagttttact tctcttcttc ttttttcttt tcatcactgc
     4141 attgtccgct gtacgggcat gccgaacact tattgtgtga gccgcccttt tttctgcttt
     4201 ttataaggta caagactatt gataaaacta ttaaagcaac aattgctatt aaaatataat
     4261 ctaaaggttt catttgtcac cccgctaaca ttgcaatctg ataaaacacc atagctacga
     4321 tatatgcaaa acccgtctga aagactatgg atataaacat atacttggtt cccaattcac
     4381 gtctgaccgc ggcaaccgcc gcaacacacg gcgtataaag aagagtaaac aacagaaagg
     4441 aaaatgcgga gatcggagtg aaaagggtag ctaacggtgc cgacagtttt tctattcccg
     4501 tacttaataa taccgctaat gtggatacta ccgcttcttt tgccataaaa ccggttaaaa
     4561 gggcagtgga agccttccag ttgccgaatc ccaaaggagt gaaaacagga gctataagcg
     4621 agcctattgc ggcaagcata ctgtctttac tgtcggaaac aacattgaat cggatatcga
     4681 atttattcaa aaaccatatc actatggatg cgacaaatat tatggtaaat gctcttgtta
     4741 tgaagtcttt tgtcttttcc cataataaaa gaaaggtatt ctttaccgtg ggcagtctgt
     4801 agttgggaag ctccattaca aagggaatgg gatttccttt gaataggaac tttttggata
     4861 tgagagctac taatatggca agcaatatac ctattaaata cattgcggtc ataaccaaag
     4921 cgcgatgttc ggtgaaaaaa gccattgtaa aaaccgcata tatgggcaac tttgccgaac
     4981 agctcatgaa aggagtaaga aaaatcgtca tctttttgtc tctgtcggaa gtcagtgtcc
     5041 ttgtagccat tatggcaggt accgtacagc cgaaacctat aagcatggga actatacttt
     5101 ttccggataa tccgattttt cgtagcagtt tatccataat aaatgcaact cttgccatgt
     5161 atcccgaatc ttccagtaat gataaaagaa aaaacagaac aatgattgta gggataaagg
     5221 aaagtacgct tccaacaccc gcaaaaaccc cgtcaattac cagcgattcc acaacgggat
     5281 ttataccgaa gtcggtcaga ccttttgaca ctatagctgc cagagaatct atcgctcccg
     5341 ataaaaggtc ggaaaggtaa ctgcccacaa ctccgaatgt cagccagaaa atgagcagca
     5401 ttatgcctaa gaatatggga ataccgaata tcttgtgagt cagtacattg tcaattttta
     5461 tagtcctgtc atggtgttta tcacacttgg ggcgggtgac tgctttttta catacatcct
     5521 ctatgtattg atatcgcata tcggcaattg ccgccatcct gtctaaggat agttcgtttt
     5581 ccatttcggt tatagcatgc tctatcatct ctatttcatt ttcccctaaa gcgaggtctg
     5641 ccatcatggg ttcatcgcct tcaataatct tgacggccgc aaaacttgcg ggcacatttg
     5701 ctctttgagc atggtcttcc agaatatgtg ccgttgcatg caatgtcctg tgaaccgcac
     5761 ccgtacagaa atcctgtttg ggaggggtgc atctgttatc taccagatct ttcaatgcct
     5821 gcataagatc ggttatgcct tcatttttat tcgcacatac gggtatgata ggaaccttta
     5881 aatattcact gaggatatcg gtctttatga ctattccgtt tgcatacact tcgtccatca
     5941 tatttatcgc tataaccata gggatgttga gcagcaataa ttgtaaggaa agataaagac
     6001 ctctctccag tgaggtgccg tcaattatat ttataatgcc gtcaggtcgc tgttttatga
     6061 gatagtcccg cgtaagtatt tcttcgggag aatagggaga taaagagtat atgcccggaa
     6121 ggtcaattat ctttatactc ttatcggctt tatatatacc ttcttttaca tccactgtga
     6181 cgccggggaa attgcccaca cgctgattgg aacctgtcag ctgattgaag agagtggttt
     6241 taccggaatt ctgattgcct gccagcgcta taatcatacg cttatctcct caatatcaat
     6301 catggacgca tcttccagcc ttaatgtcag ttcataacct cttaaatata tacataccgg
     6361 atcgcccata ggggctttcc tttgcatatg cacgataacc cttggggtaa gacccatatc
     6421 cagaaggcga tggcgtaatc tgccgtgacc tctgaccgca attattctcc ctgatttacc
     6481 gacgccgagt ttgtccaatg tcatacaatt tctccatatc cggtatgttc tgatacatgc
     6541 ctaaacatgt aaatttgcat accaatgata gaatatctta agattatcgg caagtcaatg
     6601 agcagccgct aactctttga aaaatccttt tgtttcattg acgatgttag tcggtaagtg
     6661 ttgccttgat acaacaaaaa tggcataatg tacacatggc tgtcaggctg tttcgggtct
     6721 gatttcctgc atcacttacg gtattgaata aaggaggatt ggaaatggca tataaatggt
     6781 gcggacattt tgacaatgag ggtaagaatt atgtaataac aacaccggat acaccgaggc
     6841 attggtataa ctatctgtat aacgatgaat atataacttt cacatcacag gtaggattcg
     6901 gagaaggatt tgcacaggac aaaatgggaa gacgtatacc tttggttgta aaccgtaact
     6961 tctttctgtg tgaggatgat tactggtcat tggccggtct tcctatcgga tacggatata
     7021 ccgattactc ctgcactcat acggcgggat ggtcggtaat agctcttaaa tacaaggata
     7081 tatcctcaaa ggtcaggata tttgttccaa acaccggtca tatggaaata tggagcataa
     7141 ccttgaagaa tcaatcggac aggacaagaa aactgtcgct tgttgcatat gcaaagaccg
     7201 atattgacgc tccgtataaa ccccagggct ataacatagc aaggggaagt gccttcaaag
     7261 ataaaaactg tgtgtacgga tttttctata cacaattcaa taccgaaaga aacattccga
     7321 tatacggtta tatgtcttct tcagagagca taaaagggta tgattcgaga aggacggctt
     7381 ttataggggc atataacgat gatcagaggc ccaaagcttt ggaagagacc aaaggctgca
     7441 caaacagtga ttgtatggtg gagaagttat gcctggcctt ggaaaactcc gttacactta
     7501 agcccggtga ggaaaaaaca attcattatg ttgtgggtct ggctttcggt gaggagaaaa
     7561 taataacgcc gaaggataaa tgggtgcagc agcaatttgc cgccatggct gacaaatatg
     7621 aggaccgctt aagaaggatt aagataaaaa ctccctggga gaactttgat aatttaatca
     7681 acggctggat ggtatatgcc accgatatgg gaagcagatg ggcgcgagtg cgacataacg
     7741 gttaccgtga tatgtgtcag gataccgacg gtctgtctgt tctcaacccc gaactggcat
     7801 gggagagaat aaagagggta ttgtcctacc agtatgaaaa cggatatgct ccgagaacca
     7861 taattgacgg ggaactgaaa gacaggaaat tttccgacaa taccgtatgg ctgacctaca
     7921 cagtctacag ctgtataaag gagcttaaca agccggaact gttgcatgag caggttccgt
     7981 tcaataacgg cacatccgct tcggtatatg agcacataaa aagaagtgtg gagtttttat
     8041 tcaacttcac cggtctttac gggcttatca ggatatgggg cggagactgg aatgactgca
     8101 tcaactttgc cggactggaa ggtaaaggcg tatcggtatg gctttccatc gcatggtgtg
     8161 cggcggcaaa acagctttgt gaactggcag gcatgctggg ttatgagcag gataaaaaag
     8221 attacacgga aaagtaccat ataatgcgtg acaggattga tgagcacggc tgggatggag
     8281 aatactacat gtatgcaaga accgatgaag atattaccat gggatcacat actcaggaag
     8341 agggtaagat tttcttaaac ccgcagattt gggcggtttt tgcgggattg gataaagatg
     8401 acaggcatat aaagtccatg gatatggcgg aaaaactgtt gcaatcccct gaaggaatac
     8461 tgttatccgc acccgcttat acaagacagt atcattacat cggttccatg gcggaaaaac
     8521 ccggcggagt acaggacaac ggaggcgtat atttacatcc cgcagcatgg aaacttgcgg
     8581 ccgactcgtt attgaaacgc aatgataaag tggaagaaaa ccttaaaaag atgctgcctt
     8641 tcgatcagac ctattacccg acaaaaggtg agccttactg tatgttcaac tgctactatt
     8701 tagattatca cggatacagg ggaggaactc ccggacagtc gtggagaacg ggaaccaacg
     8761 catggctttt acagtctacg gtcaagtata ttttcggttt ggttcccgag cccgaaggct
     8821 tgaaactcaa tccctgtccg ccgccttcct ggaaagagtg ttccataacc aaactgttca
     8881 gaggatgcac ttataacata aagtacatac aaaaaggtaa agggcataac aacaggataa
     8941 caaggatatt aatgaacggt aaggaaacac ggtttaagga taatacaata cctcctgttg
     9001 agggagcaac actggatatt gttgtgtatc tggaaaatta gccctataca acataagtat
     9061 gcttatagta ttctaaatat accgatatat ttccaaaagc gaatggatga caggtacggt
     9121 tatttcatac tgattggatg attccagcca ctcggcagtt tcgctttgtc tttccttatt
     9181 tatgtaccat agggcatcca tctgtacgtt tatggcgcct aagatgtcgg tggtatacaa
     9241 atctcctatg aaaagacatt ggttaccgga aagattcaat gtttcaagca tatggttgta
     9301 gaactgtttc atgggtttta cacaacccat ttcctcggat atgaaaagat gggacaaata
     9361 ctcctttacc ggactcaatc gcgattgctg tatttctttt attccgtttg tagcagccgc
     9421 cactatatgt gtttttgagc aatgtttgag aacatcaatt gcatgatttt caaatatatg
     9481 agtgttattg aatatctcgt tatactcatc ggcaatatct gaagcatgct cttttcttaa
     9541 acttcctata ttcaatttat ccaaaaatac ttcaaagcgt ttgtagccgt atattttata
     9601 tacttcatgg tatttttcct gcataaccgg gtcatctaaa agattcatat tataagactt
     9661 ccacaaatca taacagaccg ttctgaacaa ctcttcaatt tttggtgtgt actcgacatt
     9721 atggtttaaa agcaaatctt ccagtgcctt agccgccgat gcgtcatatt gtaaaattgt
     9781 attgtcaacg tcaaatataa ggcatttata tttactcaaa gtgcgctccg ttcaatcgtc
     9841 ggcagttggc gttaatataa ttattattct tgccgcgata tttttatttt tgtctattct
     9901 accaaatcaa tgttatttta acaatagaaa taaaatgttg tttttgatgt tgactttaat
     9961 tgccgggtaa ccgataataa ccgggtaagg ggtggaggag gtaaaaatat gacagaggac
    10021 atcactcata tattaaccaa caaacctgta ccggaagaga tagcagaaag acttgaaaag
    10081 tcggcggatg tgcagcgcct gctttttttt attgtcggag acctgtccaa agacagctta
    10141 tacagcgaat ctgttcttgc ggttaccgac aagggtatgt actgttttga caggacctat
    10201 gatgacggat ataaatatgt aactcatgaa aatattgaaa aaatagaaat aaaaaggctg
    10261 tacggcaatg ccataatgga aatatccgca gaaaaagaag gcactttcga ctttttccgt
    10321 tttacctatt cggttgccaa tttatgtgaa gccgcagctg atttttccga gcagataaaa
    10381 aaaggtgaga atattgacag attaatggaa atggttacag ccgtttttga aaagcagctt
    10441 tccaactgcc ccagatgcgg aaggactctt gtgcgtcccg gtgccgaatg ccttaactgt
    10501 cagtccaaag gtaaaatagc caaaaaactt atgaagtatg tgcttcccca gaagaagttg
    10561 ctcataatct gcctcatatt ctccataata accacagcca tgtcgttggt cccgccatat
    10621 ataaccaaga tgatggtgga tgacattata cccgacaata acaaaaagct gcttttaacg
    10681 gtggtatttg tgctgttttg tacttatgtc atacagtatt cggtaggtgc attgcgttct
    10741 tatctgttaa gggtaagcgg agacaggata gtggccggtt taagaagcga tatttataca
    10801 aaagctcagt atcttcccat gaagttctat gacaagacat ctaccggctc ggtcataacc
    10861 agaataagca gcgataccgc cacattgcag tcctttacct taagggtaac ccaggaagta
    10921 atagtgcagt tctttctgct tatagggata atagtgatta tgtttgtcat ggacagaagg
    10981 cttacattgc tttcgcttat tcccgttcct ctggttgtaa taggttccag aatattcgga
    11041 aagaagatac tgccttatta cagaaggata tggagaaaat ggtcaaaagt gtcctccatt
    11101 ctgaccgata ccatacccgg agtgagagtt ataaaggcat ttaccaacga aaagggagcc
    11161 attgacaagt ttgataaata caacgaagaa tggctgaaga ccgatataaa agccgcaaga
    11221 ataactactt tgtttccgca gctggtaagc tttttcgtaa cctgcggctc cttacttatc
    11281 tggagtgtgg gaggcaatct ggtaatgcat ccccaaagcg ggttgacatt aggtcttctg
    11341 gtctccttta tttcctacac ggctatgttc tacggaccgg taaacttttt tgcaaacttc
    11401 aatgactctc tgcaggctgc cgtagccgcc gcggagaggg tttttgatat tatcgatgcc
    11461 gaaccggaag cggatttcgg gaagggaaat catccgaaaa aactggaagg gaaaatacag
    11521 tttaagaatg ttaatttctc ttttgaccgc acaaagatga ccttgcagga tataaacata
    11581 gatattgaac cgggagatat tgtaggtatt gtgggtacta ccggttcggg aaaatccaca
    11641 cttatcaatc taatcatgcg ttattatgat aattatgacg gtaagattct tatggacgga
    11701 caggacataa aagatataga catggaattt taccggtctc agataggata tgttcagcag
    11761 gaacccatga tgttccacga tacgatatac aacaatatcg cttacggcaa cagcgatgtt
    11821 caggtagagg aagttatcca tgccgcaaaa atagccaatg cccataactt catagtaaaa
    11881 ctgcccgacg catatgatac cgtgctggga gaaagaggta ccggactgtc cggcggtgag
    11941 cgacagcgtg tatccattgc cagagcggtc ctcaggaatc cttgcataat gatattcgat
    12001 gaagctacag ctgcggtgga ttcggaaacc gaacttctta tacaggaagc cattgaaaga
    12061 ctgataaagg gaagaaccac acttatgatt gcccacaggc tgtcgacatt aagaaaagcc
    12121 aacaagattc tggttgtgga cgaaggcaga ataattgaaa gcgggacgcc cgaagaactg
    12181 ctggcaaaga agggtaaata ctacaaactt attgaaatac agacaatgtc gtccaaactg
    12241 caaaaagcca aagaagagga aaacgtggaa taggaggaag catcatgaaa gtaccgtata
    12301 tacaaggcga taatgccaaa attgaaagag cggatttgac acatgtgaat gtaaccatgt
    12361 atgacggacg gaaatttgag aatgttcagc ccagaaggct ttttcccata agcggtctga
    12421 gaaaatacat cactcttctg gattttgaag aaaaggaagt tgcgattata agaaacctgg
    12481 atacattaat ggaagattcc aaaaatgccg tggatcagtg cttgaaccag tattacctgg
    12541 tacctaagat aatcaggttg tatgagatta aggagatatc tggtaatatt aacatgcatg
    12601 tgctgacaga caaaggggaa agaaagtttg aaatatggca cagatataac gatataaaga
    12661 ttattcacgg cacaagaatg ttgtttagag acactgcgga caacagatat gaaatagaag
    12721 atattgacaa gcttgacaaa gtaagcaggg caaagcttgc cacatttata tgatataatg
    12781 cttaacggag gaccaaatga aacttatttt agctattgta aataatgatg acagcgctat
    12841 tgcggcatcg gctcttacgg aggccggcta tttcgtaaca aagctttcca ctaccggagg
    12901 atttttaatg gtaggtaata ccactttgct tatcggtacc gaaagcaagg gaaccgagca
    12961 tgttatagaa atactttcca agtactgcaa aacccgcaag cagaccgttc tgtcaacggc
    13021 atcgttcgga gtgggattat ccaatgaaag ccttcctccc gaagtaacgg taggcggtgc
    13081 tgcgatattt gtattgaatg tagagaaaaa tattaagata taggctattt cttttttctg
    13141 tcttgaactt tttcggagcc tgcagatgta gcaggctttt ttgtaaataa tttaaccagt
    13201 ataaaacctg caaacatacc caatgcggaa accgcggcat aaagagcggc atatagcaac
    13261 gccgagtagt cgtagtatat aaaagcggcc ggaacaaaca gtacggcggg aaacagtatg
    13321 tacagccact taaatccgtt aaacagtccg aatatgatgg aagctatgta tataactgcg
    13381 gggaaaccca gaataagcat atataccaga aaaccttcaa tatgcgatcc cataaaagcg
    13441 acaggcagaa ggttgaacat aaaagcataa aatgcaatat gaactatata gtggttttta
    13501 agtcttaccg atttttccat atcggtcatc tgattaaatt tctttctcat agattgattt
    13561 tatagataac acaaatatat tacaagtcta aaagggttat cttccgttaa gttccttcca
    13621 ttcttctttt aacaaagagt atgtcacaat atccacaggt tcgtttctat atgaaagacg
    13681 ggctttacgt aatgtacctt ctcttttaaa accgcatttt tctattacct tcatggaacg
    13741 gctgttatga ggataatgat cgacggataa aaggtccagc ttcattacgt tgaatgcata
    13801 ttcttgtatg gctttaaccg cttcagtggc tatacccatg ccccagtagt cataagagag
    13861 agcatatccc aaacatcttg tctttaaatc ttttatctgt gagcgaagaa tgtcgtcatg
    13921 cagaccgatg gagcctatta ctttatggtt ctctttgtag acaattgccc aaagattatc
    13981 ggccctcata aacatattca aaacagattc cgattcctgt atatttttat gcggtgtcca
    14041 tcccgcatta ggtcctacat tgggattact tgcatattcg tacaagtctt tgacatcgtc
    14101 aaatctgaat tcgcgaagga tgagtctttc ggtttcaaca gtagcaacca tggtacctcc
    14161 ttatttatct ttccataaag tgttttaata ttattcttac cctaacctgt gttttacacc
    14221 ttgctgtacc agatgcagat tatttgtaag tatggtatct attcctctgt ctaaaaatcc
    14281 tgaagcttct tttgggtcat ccgaccagaa tatgttgcat atgataccgt tgtccttggc
    14341 ctttttaatc atttcatcat tgaaatacgg tttgaagaac tgcagtttat cgcatttata
    14401 cttaatagcc ttatccacga gagtatagtc cagctgtccg tcaagacaac atcttttcag
    14461 atgaggagct accttcaaag ccgtttccat taccgcttca tctcctgcga tatagatata
    14521 gtcggcacag tcgtattttt ctatcaaatc cactatgact ttgaaatcgt cttcatcgta
    14581 gggaaaaacg atattggagt atttatcaat aaacttggtc tttatatgta aattcataat
    14641 agtctgatga tggaatcttt tgagtatatc ttcaaatgtg tcatatttca ttcctgcaat
    14701 cataggactc atgcccatag aagcatccag ctccattacc tcttcaaagg tatattccca
    14761 gacctttttg gatttgtcat gcgaaaaatg cggatcatgc atggcaatca taactctgtc
    14821 tttggtaggc caaacatcca gttcaatctc atctgctccc aaagcaactg ccgccgatat
    14881 ggccagcatg gtgttttccg gacacaatgc ggaaaatcct ctgtgagcac agatacgggg
    14941 ataaggtttt tcattatcac ttttagaaat aaaactgcct gtcgcaagat aagcatgagg
    15001 tgttcttccc tgtgttacga attcatcggt cggtatgaga ggctgaccgt gaccgttggg
    15061 tttcattgct tttttaggta ctttaatatc cgctcttaaa actcccgtca actgttccat
    15121 atctgccaga accgttccgt cacagtcgca gacaagtgag tgaccgcctt tggcggtatt
    15181 acccatggag taggatgctc tcaagacata agcattgcat ctggaggata ttatccttgc
    15241 ctgtgaaaga ataatgtctt cttcttcact ccgctgatag gaacagataa gaatgatatc
    15301 gggtttcttt gaccggatat actcgatata ctctaaaaag tatgtatcgt aacaggtcat
    15361 gaatgccagt ctcagtccgc ctacatcaac aaagcagggg atatcggttc tgtatctgta
    15421 agagtaatcc agttttaaca actctttttc cgcatatgta agatgctgtt tgtcatatct
    15481 gtaaataagc tctccgcttc cgttatataa aagcgtgctg tttcggaaac cggtatcggt
    15541 cttatgtgcc atattgacgg ctacaaggct gtttgttctt tttgccgtgg cgataactct
    15601 cttagtcaga atatcggtat tccggttgta atctgcaatc atttcatcat actgtgatgc
    15661 tccgggaata ttggcaaatt cggggagcag tataaggtcg gcatccgttc ctacggaatc
    15721 cagcgattgc agtataaagc caacagtgtt tttcttttca tcaccactct tcggataaga
    15781 cggttgtaaa acatatactt tcatagttac ctctctaaat catttttact gtcaacaatt
    15841 aattccattc ttctgtattt aacggtaaat ccgtgatccg catagaattt tacggctctt
    15901 tcgttaatct cccaggcatt gagttctacg gcatcacagt gctctttttt tgcgtattca
    15961 tatgcgtgat ttatgaggag ggagccgata ccgatacctc ttttatcttc atccacacat
    16021 aaatcatcca aatacaatat ttttctgttc tgctgaacgg tatgattttt aactatgtca
    16081 aacacacaga ataaatatcc agcaactttg tcggtttcat ccaccgcaac ggctatatat
    16141 tcttccggtc tcttaatctt ttcaagtatc aatttattat tgtatttact ggcattgctc
    16201 ttaaatatat ccggtcgctt ttcatgatgc agttgtgcta tctgcccaag cagcctcagg
    16261 atatcttccg catctttttc ttttgctctt cttatatgat attgcattta caatgtctca
    16321 tttccatatc ttctatattc tttattatat cccataaatc tttatttaga tgtattattt
    16381 gaggctacaa gggttaaatg ttttctggca taaaaatggc ttaaacatat gataggctat
    16441 attattgttc atgtggtaat atagattgtg tggatagtat aaaggtttag atagttctat
    16501 ataacattta tttctttttc caactcagag aggcgattca taattgtatt aaatgtagga
    16561 atttcaccgt aaagcatatt tctcatgttc tgataatctt ctgataaagc gggtatccgg
    16621 taagatggtg gaatgagttt cagtgttccc ctttttgctt cctgatattt tgcccatgca
    16681 cgaggataaa acttcatttt aaattctgct acctttgtta atagctccaa actatcaaaa
    16741 gcggtagatt ttacaggagt gtccgccata cggtaaaggt cgtagtaatg tctcgaatat
    16801 ctctttggca ttggcagatt tttaggtctg tttgcctcgt gatgaaggat tgttgctttt
    16861 tcccaaaaag tgcgttccgg tagaacagtg agaacctcgg ttgttttttg acagaatact
    16921 gatgaataat gttttgctgc atatgattct ataacaacct tacgtgcagg tgtccatgca
    16981 gcaagtgggc caatttccag tcggattact ttcaatgtgg aagcattttc aaacagattc
    17041 ggataagcaa aaagaatagt ttgcttgtct tttttatcta tatagatatt tgtcgcagaa
    17101 tttaattcct gtgataagtc ggatttcatt ttcggacata cagtttcatt caagaattct
    17161 tccgttctgg tatttgcttc tttattaaag aggtcctgtt ttgtattaga acggacaatc
    17221 cacggctcgt caaccccata tccgagcaat ctccagtcca atatcagatc aatgtcttct
    17281 gaaaaacggt taataagatt gtatgctttt gaaaggctgg ttcctccttt aaatgataag
    17341 tgttccttcc atggagagcg gtggaaaaga taatccagca tgaaacatac ccaaaaatct
    17401 ttttcaacta tagcatcgtt aagtcccatt ttgtcggcag tatttctgaa tagctctcgc
    17461 cgctcatcag ctgatagttt tgctactgtt ttcatattgc ttaaacctca cttatctgtt
    17521 ttattattct aaaaatccaa tcggcacttc ttgatgcttc ttttagggct gcatcttttt
    17581 ccttccgtgt caatttctcc gaaagtgtct caataatgtc ttgcgtaatg ctttctttac
    17641 caagtgtttt aatagcttga atcaaaagag cagtcatata ggatagaccg gtaatctctt
    17701 tgtttgttct gtgcttaaat tctatgcatg tcgagttcca ctcatacttt ctatatggtc
    17761 cgtcgctgat atatgaccag gtggccggaa cctgcgttga aagacctatc atattcaagg
    17821 ctgtattacc gcaaggagca attgtccaat ggtaactgcg ggcaagtgcc tgtgccaccg
    17881 catcgggatt aacagctaca tactctccaa gcaatttgct gtatttaggc ttttcatata
    17941 caccgcctaa aatgcgcttc aaaacacctt cgtttacgag acgaagcata ttttgccgaa
    18001 tagtattatt atcggcaata tctgaaaagt ccgatgtagt aaagactgtt ccatttgttg
    18061 ctgaaagcac gcgctccctt attttgtctg catatccgtt actcataaga gtaccctcct
    18121 tgtaacgaaa attatatact attcgttaca agaaagcaat aatatctcaa aagtaattac
    18181 cggattattt aagaacaagc ttttcagagt tcttatggtt gttgcaagat ttatacaatg
    18241 tattccccat aaatcagcat ttagatgtat aatatgagat atgaggagaa ttgattatga
    18301 taataacccc taaatacttt aaagacaaat tacccaaagc ccttaccatg agttatgatg
    18361 acggaccgta tgaagattac aggcttgtgg aactgtttaa caaatacaaa ataaagggaa
    18421 cattccatct taattcacga tacatggaca gagacgatta tcctccgcag ccggaaaaga
    18481 taaagatgac cgatgcgaag aaactctacg aaggacatga aatatcggta cataccgtta
    18541 atcatgcaga tttgcgagat gtcaataatc tgaggttttt acatgaagta ttggacgata
    18601 aaaaggcact ggaagatatt gcgggatatc cggtaagagg tatgtcttat ccgtacggtt
    18661 attacaatga tgaagttgta cagcgtttaa gatatttaga tatggaatat gccagaacca
    18721 ccaaaagcca taagtccttc tacttacccg atgattttct ggtatggcat ccgacatgcc
    18781 atcacaacga tgcaaccgac cttatatccg cattcgacag tcccagatgg aatatgggaa
    18841 tgaatctttt ctatgtatgg ggacatagtt acgagtttga aagatggggt gacaaatggt
    18901 acataataga ggagttctgt aaacgtataa gcggaagaga ggacatatgg tatgccacca
    18961 atatcgaaat aaaggattat gtcgatgctg tcagaaatct gaaattcact gtggatactt
    19021 ccatggtata taatcccagc tgtaccgatg tctggctgga tgtggacaga gaaccggtaa
    19081 aggttgctgc ggggcaatta acaaagctta aataaaaccg tgttctactg ttatatcaca
    19141 ttttacttga ggattggttt ttgccaattc tttttttata ttttccgaaa gagtttcttc
    19201 ctcctcttgg gtataggagt gagctaccgt aacatcaaat accagattta tctcattttt
    19261 atcgaacttt gcattaaagc cgtgaacagc ggcatcggga tccagttttt tcactatacc
    19321 tttaacaacg gatttgtatt tgaagaatct cttgtcatgc acttcctgag gatccatgtg
    19381 tataacaaga tgtattcccg tttccctgta aacatccttt tctattccgt ctatgatgtc
    19441 atgcattttc tgcatatcac cgtcattggg aacctgagca tgaatggtag ccattatatt
    19501 cgaaggtccg tagctgtgaa caataaggtc gtgggtacct attattccgt catagctctc
    19561 cactttaccg gtaattttat catacagttt tttgtccgtt gccgaaccca aaagcggcat
    19621 aagagtgtct ttggctatct ttaatcccga aagaaaaaca aataccgata aacctatacc
    19681 cgcataactg tctatggaaa agttaaaata ttttgcaata agcatggata ttacggttac
    19741 cgaggtggca accagatcat ttgccgaatc tcggaatgca gctttcaaag gataggagtt
    19801 tattcttata cctatatctc tgtttaacag cattacccag gctttaagca gcatacttac
    19861 aattaaagct attacaaaag cccattcaaa tattatgggc tccggttgag ttattctttt
    19921 tatggaatcg acgaacagcg atatacccgc attcattatg ataaaggaca taaaaagagc
    19981 cgccacatac tcaatccttt catgaccgaa aggatgctct ttgtcggcag gctttttgga
    20041 aagttttgac gccagtaagg ttattaaaaa agaaccggca tcggacagat tattgaaaga
    20101 atccgccagc accgcgatac ttttggataa cagaccgata aaaagcttgc ctgtaaaaag
    20161 cagtatattt acagtcagtc ccgatacgct cgcaagcatt ccgtatgcgc ttcttaccga
    20221 tgcatcatca gtgtttttat agtcttttac gaacaacttt atcagcaact tgttcatgga
    20281 gatatcctgt taagttttat gtcggctaat tgctataact tcttaagttt acttgccgcg
    20341 ggtttgtcca gtattaccgt aacatcacgt ttgaatatct gaatagccga agcggtaatc
    20401 tgagttgtaa ccggaccttc cagagctttc tgaattatat ccgccttacc gattccgctt
    20461 acaatcataa gaagactctt tgcctccata atagtggcaa cacccattgt tatggctttt
    20521 ttgggcattt cttctttgga tacgcccgct tttttgtaga aagcttcata attatcgtct
    20581 atggatttct gattaaggtg ctccactctg gttctggaat caaaggatga accgggctca
    20641 ttgaatccta tgtgaccgtc tccgccgata cctaaaagct gtaaatccaa tccgccggca
    20701 tccgctatca tcttttcata ttctttgcag tatttatccg gatcggttgc aagcccgtat
    20761 ggcacatgag catttttctt tttgatgttt atcttcttaa ataactcttc atgcatgaac
    20821 cgggcatagg actggtccat ctcatacggc agcgttagat ccatggatat tcccagatat
    20881 tcatccagat tgaatgttgt gactgcagaa aagtccagac cttcctcttt atgcagcctg
    20941 gcaagttcat gataggttcc gataggtgta gcgccggttg ccagtcccaa tacgcagtcg
    21001 ggtttctttc ttaccagatc ggcaataacc gaagctgcca atttgctcat ctcgctataa
    21061 ttatctttta caataatttt catagcaatc cttctttctt ttatttaatt ttattcatct
    21121 gttcttgacg gtatactgaa aagaacgtct cttcagaaac aattaagccc agggattttc
    21181 gctttccggt ttacgtatat cggcaaggag atactgttcc caaagatacc atttcccgtc
    21241 ctttgcttta cgaagcgata ccagacgcgg actgtctgct ccgcccgaat taatatacag
    21301 tctaacggta tccggatcgt caaatgaacg gggattggaa gtaaccgtta ttgtataggg
    21361 ctttgacggc atatagtcgt tggcaggtgt tgcaccgtcg aagtaagaac gcggaacata
    21421 atctttatcc aaaaagcggt ctcttataaa ctgcttatcc atttcgctca tcgggcgcgg
    21481 tccgcgtaaa taattcagca tctgatggca caattcctta tttacgggat agaggcagaa
    21541 tgccacaacg gtaagtgccg ccgtctcaaa cggtgatgca agctgcgcct cggggagtgc
    21601 tttaaatgct tccaatgttt caggcagatc ggcaaaagta atcttctgag ttttgtgtct
    21661 cggatcgaga gctttttcta tcgctgatgt agcctgtttt ttcaatttgt caaaaaatgc
    21721 catgattctc tccttttact ttttttatac ttctgtactg acggaaggat atcggcatac
    21781 tgtcttacac cgagaacttc cttattatag gatatcaaaa tttgcctcat ttttcaatat
    21841 tcttgcgtaa agttcaggca atctctttac cgaaatccgc cgctttttgc agtgtttcgt
    21901 aatcgggatt ccacaggcac ttaaaaccgt catttattac ttcaaatccc gcatcggtaa
    21961 gcatttcgtt caatatctta accgattctc ccgaccagcc gtaacagccg aatgccgcag
    22021 cctttttgtt cttcaacttc agacctttaa tgtagtgggt gagtgcggca agcgaatgca
    22081 gtgcacagtt attgactgta ggagaaccta aaagtaccaa cttggattta aatatctgtg
    22141 tggcaacatc attcatgtca ctcttaggca gattgaatat gcgtatattt acactctcat
    22201 ctttttccct tataccctct gctatctttt cggcaagcgc tctggttccg ttccacatgg
    22261 tatcgtaaac aatggttatg aggttttcct gatagttgtc acaccataac ctgtattttt
    22321 ccactatctg cataggattg tctctccata tcacaccgtg actgggggca atcatatcta
    22381 tatccagatt aagtgattgt atttcttcca gttttctttt gagtatcgca ctgaagggag
    22441 ccagaatgtt tgcgtaatat ttttccgctt cggcatacag tgtgcattga tctgccgaat
    22501 cgttaaagag atggtcgcaa gcatagtgct gaccgaatgc atcattagaa aacagtatat
    22561 tgtcttttgt caggtaacag gccatactgt ccggccagtg cagcatacgc atttccacaa
    22621 agaccaactg cttgccgtta cctatgtcta tggtatctcc ggtttttaaa ggccgcaaat
    22681 cccagttctt aaaatattgt ccctgcagtg attttacggc attggcggag cagtatatgg
    22741 gagtggaggg tattttctcc atcaacttgt taagtgctcc gctgtggtcc acttcaccgt
    22801 gattgcacac tatgaaatca atctttttta agtcaatttc cttttccaga ttttccacaa
    22861 attccttttc aaacggagac catacggtat ctatgaggac atttttttcc tccattatta
    22921 aataactgtt ataacttgac ccgtgatgag tggataactc ttcaccgtga aatttcttca
    22981 gttcccaatc ggtctttcct acccaatata cgttattctt taccggcttc ttcataaaaa
    23041 tccctttctt gcagatattg tatatatcat ttcaatattt gaattaataa atacttgttt
    23101 aaacaaatta tatcacacac aataaacagt ttaagtgagt atgtcaccgt gacctgtttt
    23161 gtacttagtt gaagagaaac atttgtagat aagattatat aatatcattg ccaaattact
    23221 gaacaagtga tacaataatt ctgaaagaga ggtacgtgat gggaaagtcc gtatacagcc
    23281 tcatacttaa cgatgaggta ataaaaaaga ttgatacgct ggcatatgca aaaaggacca
    23341 gccgctccgg ttatataaac gaggtactgg catctcatgt gtcctatacc actcctcagc
    23401 agaggataaa agacatcctg aatgccgcaa gagctttttt ggaaccgtac gaaaaatatg
    23461 ctttcgtgga gatgaactcc aattccttta tggatgtgcg aacggcattg agctacaggt
    23521 accgtccgac aatacgctat tgcttggaga ttctgggaag ggacaaaggg ccttttctca
    23581 agttgaaggc gcaggttcgg acacaaagca gcagtcttat ctcagcgata gaagacttct
    23641 ttaccatatg gcagaatgtc gaaaagaaac ttatacccga cacatatgat gaggtggaga
    23701 tgacatccta tgaaaatgtc tgctatacga gatttttctt cttaaacgac cgtatgaata
    23761 ttgaagagca aaggcttggc aaagccattg cggcatatat aacaacattg gataaggcat
    23821 tggatatttt tatgagtaat atggataata ccgattatgt tatatcggat atatatgcgg
    23881 catacaaaga atactatgca aaaacaggaa tgattatatg atttaaggag gtaaaacatg
    23941 aatatacaga agtttacaca aaaatccgta gaagcactta acagggcaca aagccttgct
    24001 attgaatacg gtaatataca gatagagcag gagcatcttc tctatgttct tttaagtcaa
    24061 ccggaaagct ttatagcaca gttaatggtt tccatgaact taaataccgg gttacttata
    24121 caaaaagccg agaacctggt atccgcactt cccaaggtaa cgggaagcgg cagggaaccg
    24181 gataaggtat atatttcaaa ggataccgat ctgacactaa atgaagcgga aaaacaggca
    24241 aagataatga aagacgagta tgtgtcggta gagcacatat tccttgccgt tattgaaacg
    24301 gccaatccga ggataagaga gctgttcaga gaaacaggca taaccaaaga ttccttttta
    24361 agagcactgg cttccataag aggaaacacc aaggtgacat cggataatcc cgagtctaca
    24421 tatgatgtgc tgaaaaaata cggccaggat ctggtggaac ttgcaaggaa tcagaaactg
    24481 gacccggtaa tagggcgtga cgaagagata aggaatgtta taaggatact gtccagaaag
    24541 accaagaata atccggtact gataggtgag ccgggtgtgg gaaagaccgc tattgccgaa
    24601 ggactggcat tgagaatagt acgaaaggac gtgccggaca atctgaagga gagaaagata
    24661 ttctccctgg atatgggtgc actggtagca ggagcaaaat acagagggga atttgaagag
    24721 cgattcaaag cggtacttca ggaagtaaag aattccgaag gaagaataat actttttatt
    24781 gatgaattac acaatatagt gggagcgggt aaaaccgagg gagccatgga tgcgggcaat
    24841 ctgctcaaac ccatgcttgc cagaggagaa ctccattgta taggtgcgac gacattgaac
    24901 gagtatcgtc agtatataga aaaagatgcc gctctggaaa gacgattcca gccggtaatg
    24961 gtaccggagc cgtctgtaga agatactatt tccatactga gaggacttaa ggaaagatat
    25021 gaagttttcc acggagttaa aatacaggat caggcattaa tagcggcggc aacactgtcc
    25081 gacagatata tatccgacag attcctgccc gataaagcca ttgatttggt ggatgaggca
    25141 tgtgccctta taaaaaccga gatagattcc atgccttcgg aactggatga tatatcgagg
    25201 aaaataatgc agtatgagat tgaggaagcg gcattgaaaa aagaaagcga caaactttcc
    25261 aaagagcagc tggccaagat aaggcaagaa cttgcccaat tgagagaaga gttcaatgag
    25321 atgaaagcca agtgggagaa tgaaaaggct tccatatcca aagtgcagaa gatacgtgag
    25381 gagatagaga aggtcaatgc cgagattgaa aaagccgaaa gagaatatga cctcaataaa
    25441 gctgccgagc ttaagtacgg aaaactgcct gcccttacaa aaacccttgc cgaacaggaa
    25501 gagatagcgg aacaggcgga acagagtcat actctgttga gagataaggt aacggaagag
    25561 gaaatagcac tgatagttgc aagatggacc ggtatacctg tatcaaaact tatgcaaagc
    25621 gaaagagaaa aacttttagg attggaagat atactgcaca aaagggttat aggacaggat
    25681 gaagcggtca caaaggtaac cgaatgcatc ttacgttcca gagcgggcat tcaggatccc
    25741 gacagaccta taggttcctt cctgttttta ggacctaccg gtgtgggtaa aaccgaactt
    25801 gcaaaggcac ttgcagaggc acttttcgac gatgaaaaga atatggtcag aatagatatg
    25861 agtgagtata tggaaaagtt ctcggtaagc agactgatag gagcgcctcc gggatatgtg
    25921 ggatatgagg aaggcggaca gcttaccgaa gcggtgcgaa gaaggccgta tacggtggtg
    25981 ctgtttgacg aaatagagaa ggcacatccc gatgtgttca acattctgct gcaggtttta
    26041 gatgacggca gaataaccga ttcccaggga cgtaccgtgg acttcaagaa caccattata
    26101 ataatgactt ccaatttggg taccccatac atcatggatg gtataacgga ggataacgaa
    26161 atatcggacg aagcaagaaa aatggtggat actcttctta agcagaagtt ccgtccggag
    26221 tttttgaaca gattggatga gatagtactg tacaagcctt tatccgaaaa ggagataatg
    26281 tccattgtag atcttatgat tgaagatctg gcaaggagag tggagaataa gaggctgggc
    26341 ctgaaggtat caaagcgggc aaaagagtat atagtagacc agggattcga cagtatttac
    26401 ggagcacgtc ctctgaaaag atatatacag agtcatgtgg agacactgat agctaaaatg
    26461 atactggcac atgatttgga gccggatacg gtattgtatg tcgattacga cggcgataaa
    26521 cttacggtag ataccgaagg ttaagtaaat gaaatgattt tagatatctt atttgaataa
    26581 aataaatgta aaagggctgt attacaacag cccttttgtt atatatcgga atagcttttt
    26641 atcagaacca tttctttttc ttgaagtaat atatcattaa caaggttata accagcataa
    26701 ctcccaatag cacgggataa gcccagggta tgttaaattc cggtatgtgc ttgaagttca
    26761 taccgtacaa tcccacaatg aaagtcagag gaatgaatat tgtggatata acggtaagca
    26821 cctttattat ctcgttaagt ttatagcttg tggaggcata aaacagctgc atcaggtcac
    26881 ttatcattac acggcaggtt tccgacaatc ctattgcttc ttttatgtga tcataaacat
    26941 cattcatata aggggcgacg gaagaggtaa tgaattttga aggcatgtta atcaagattg
    27001 agcgtgattc cttgaaaggt atgatggatt ttgccaccag ctggttatcg ctctttatct
    27061 cccttatttt tgccaacagt gagtcggacg gatctttgat tatttcctct tccaaccggt
    27121 atatccgttc ttcaatctcg taaagggttg caaagtaatc atcggttatg gagtccataa
    27181 gacggtatag cagataatcg ggaccggacg tgcgtatgcg gcttttgggg gtatgtaact
    27241 cttttcgcac tttatcaaaa acatccccgt ccagatctcc tatggaaata acgtaattgt
    27301 cgcctaacaa aaaggaaata ccctctgcca caagggcctg ttcttttttg taaatcatag
    27361 tggcaacggc aaacagaaag tcctgataat catccagttt gggactttgg tctttttcat
    27421 atatactttc caaaactacg ggatggatat tatacagtct tgacatctgc tcgcataata
    27481 ccttgccgca ttcattgtcg atatcaatcc agcgaacccc ttttacatcc tttttaagtg
    27541 tttccaaaga atctgtcgaa tattctcgaa tttcatcgga attgtattcc gttacggtaa
    27601 tattgggttt ttgtaaatta ttctcactca tttctgtgcc ttcagtatat cgcgtatttc
    27661 cgtaagaagc agctcttctt ttgtcggttc gggcggtgcc ggcggagcag gaggcgtagc
    27721 tgcagctgca gcttcttccg ctttctttgc cttttcttta agttcttcac ttttcttttt
    27781 caaagccgtg aaaatcctta ccagcatgaa cacacatgcc gacattatga gaaaatccac
    27841 aatatactgt ataaacattc cgtaattgac cgaagagagt gctttgcctt ccgcatcata
    27901 tgtcagtacc catttaagtt ccctaagatt aatttttccc attaccagac ccactaaagg
    27961 aacaatgatg tcatttgcaa gggaagacac tattttggta aatgccgatc ctatcagaat
    28021 accgaccgca agatctatta cgtttcccct tgttataaac ttcttgaact cttcaagtag
    28081 ttttgtcttt tcctttttaa cacgtttaac acgctttttc atatatacct cctgttttgt
    28141 aaatgcatat attcctatct atctcagcat tatcttttcc aattctaacg gttcgacata
    28201 tttgcctttg caatatgccc tcatgttacc gccggatatc tcatctataa gtgctatatg
    28261 attgtccgta cccactcttc cgaactccag ctttatgtca taaagctcca atcctttttt
    28321 gagcatttcc tcttttacta tagaacctgt ttttcttgtc aactccacaa gaatacgata
    28381 ttcctctttt gtcattatct tcagcatctg caacgcttca tcggttataa gagggtcatt
    28441 tctgttgtca tctttcaatg tcacttccac ataaccgtct aaatgctgtc cttcggtaca
    28501 atacattccg tatcgcctgt agaaactgcc tacagctctg tatcgcaata tgacttccac
    28561 accttttccg aacattttcg caggccggac tgtcattatc ctgttttcta tatctgcact
    28621 tatatagtgg gtgggaatgc cgagactgtt cagtttttca aagaagaact ttgtaagttt
    28681 cagtcccgct tttcccgagc cttccatttt cagtccgacg gtattggctc ccggatcgaa
    28741 aaccccgtcg gttcccgtca catcatcctt gaacttcaga agataatttc cgtcttccaa
    28801 gcagtatacg tctttagttt tacctgtata gatttttttc attctttaac ctctactgta
    28861 gtaatatagg ttccgggaaa agggctttcc ggtcaacact ttaatatcaa taaaattata
    28921 cacaattatg ttttgttatg cactatgtaa ttgatgctgt ccgtggcagt gagatgtatt
    28981 tttataagta ttattttttg attcaagaag ccatcaaaaa tcggcagatt aaaagttaga
    29041 ggatttatga gtaatgtcac aatatggtcg gcaatgtgat tgtaatgtcg ggcaatgttg
    29101 ttgcaatgtt cagaaatgtt attgcaatgt cgggcaatgt ttgatataat ttagcggagc
    29161 aatgagaaag gagagcgttt tatgaagata accgaattat tctcagatgt aataacggaa
    29221 gatatggagt gtgaattcaa agcggtactt aattcggata atcctgtgaa gtgggctaaa
    29281 agcattgtgg gttttgctaa cggtaacggc ggtatgctgt ttgtgggagt ttccgaagac
    29341 cgggaagcct tcggtatcag cttggatgaa attgataaaa caaaaaacct tgttgcaaaa
    29401 ataaatgacc gacatatttt tcctcatgtt aagttgcgct acctgatgcg aaatgttgat
    29461 aatgctgcgg agaaatttgt tcttgcaata aacatttctc cttcggaatc cattgtaagg
    29521 tatcgtgagg gtgattttaa tgaaaccgta tacataaaag gtaacggcta ttccgctccc
    29581 gcaacgccgg aagaaataat caccttatca aaaaggaaat tcggtgtgga taatgagata
    29641 agcgaaactc tttatgtaga aaagcaatgg acaagttatg ccgaattatg cagggagttc
    29701 agaaaggatg gttcggtacc cactgtaaaa gagttgcaaa actatgagat tgtttccaaa
    29761 gacggttacg caaaaacagg ttttctgatg tttagagacg gatatgacgg tgatgataca
    29821 ttaatctgct gtcgtttatg gagaggtaaa acaaaagtcg gagaagtatt ggataacggc
    29881 agattcaaag gaccgttgcc cgatgttttt aatgaaacgc tcaggtttat tgagagaaac
    29941 acaaaaacag gttggcacaa aacggaaaac ggcggtcgtg aagagattcg ttcctatcct
    30001 aaaaaagcta tacgtgaagc attggtaaat gcaatcgcac acagggatta ctctatttac
    30061 ggaacgcaga ttgatgtaga tatttactgt gacagaattg atattgtttc gcccggttcg
    30121 tggttgttac caaaaagtta tgaagaatat cctataggtt ctattccctc cgttcgcaga
    30181 aacacaatca tatccgcttg tttggatgtt gccaatctta tggagcgggg aggaaccggt
    30241 tttcaagcta tagttgacgg ttataaagac agcagtcagg acaagcagcc ttgtgtattg
    30301 atatatcccg gatttctgaa tttgcgtt
//
LOCUS       DAOSOM010000025        28873 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_1634_length_28873_cov_5.679853, whole genome shotgun sequence.
ACCESSION   DAOSOM010000025 DAOSOM010000000
VERSION     DAOSOM010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 28873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28873
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1634_length_28873_cov_5.679853"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1619)
                     /locus_tag="PLF14_06785"
     CDS             complement(<1..1619)
                     /locus_tag="PLF14_06785"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HOT71030.1"
                     /translation="MDYALILSVSLYLCGCFYLFFGIYAIYANIKSKVNRMFFYLTAT
                     LAIWAYCDSIVNSAPSAESSAFWRAFSVFGWGVFYAILIHFIIVITNNRAFLDKPRTH
                     FLIYTPAFLNIVLFSPFGILNQMQNQMTKSEFGWINAVPANFSRIWLVLYFIIYPVVG
                     VFLLLSWWKKVRPNDFIKRHLNFLFLSTVLPSVVGITANILPNIPLRYLLLKTFIFFN
                     MLPTITLFIALKKYGIFHEREKTVYVFHEGEIEGQSSRLRLFETTSIIFLIGSFVSFL
                     IGYFFMKKPLDMEIFLSASIFAIALVSKFIPYMIKKHSVQNVLFLLVCMFGMTLYSTT
                     SADEGGLTTWFVYIIFLLFTVVLGTRIHALIYTIFVIMIQVLLWIVYPQVVVVIDGSQ
                     YAVRIGIILLSFFSIRYVTREYSSKENEYKRFAKEQLVLEKISTSFISVNSGNVMEKI
                     QDMFKMSLEVLNYNHAYLVEFDNDYQDAKIFDTYVNEPDAESFPLTPGMTVTMADFPV
                     FEPLIKDKKPIMCEDIKKISLSEGEEQREFFS"
     gene            complement(1767..3140)
                     /locus_tag="PLF14_06790"
     CDS             complement(1767..3140)
                     /locus_tag="PLF14_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71031.1"
                     /translation="MAKILLNTANITCSNKKETDLKAYVNGKVINTLNYDSKEVIMEY
                     ADTDFIFLIEQNMETNDTGNDVSLMREIRRYAKKYKEYETETKFSDFEEYISTTVSYL
                     DNLEETGEIDTMNRQVSAALISHDKGLLAVTDTDTAIYISGGNIVRVFPEDDDSRIKT
                     APIPVLKTNDLIILLSETVAKALSAADILGRIDTYDSAESIAQNICSLYTSFNNEEFS
                     VTVILVKLIQDKSKVSAVSDINTQEIPTEELQKTYTSTQPLIYEDKTDKPEKKESFWD
                     DSSKDIVFTDKKTKMQKISSFDSVKLSKSESRKRRTSIYIKRAISIVVVLAVMAGIII
                     GMVNLLKLIFNRKKPVETSPVAVTSPIVVQTPTPTPTPTPTPSEEPTESPEPTIEPQY
                     ITHIVKVTTDHTSLNQIANYYNDVYSLNYPNTAIFVEVIAGLNNIKDINMIYAGQELK
                     IPITPVP"
     gene            complement(3196..3768)
                     /gene="coaE"
                     /locus_tag="PLF14_06795"
     CDS             complement(3196..3768)
                     /gene="coaE"
                     /locus_tag="PLF14_06795"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOT71032.1"
                     /translation="MIIGITGQTATGKTSLTKAFAQRGYAVFDADETYKHLLDTSVCM
                     QKELLNEFNTLDRKVILSRVIDDKESLERLNAITHKYVAEKIYEFVEENSEKNIVLDV
                     PVPVEKGFIDITDIIIVTVASKKVQLSRLMQRYGINEEQACKRIKMQKGREDYLSIGD
                     IILNTDKTDKSDLMTFTDSFENTYIKVNKD"
     gene            complement(3773..6313)
                     /gene="polA"
                     /locus_tag="PLF14_06800"
     CDS             complement(3773..6313)
                     /gene="polA"
                     /locus_tag="PLF14_06800"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOT71033.1"
                     /translation="MKKLLLIDGNSILNRAFYGMASNMLVTKDGLFTNAIYGFLNILE
                     KYLNEDNPTHIAVAFDLKGKTFRHEIYEQYKAGRKGMPNELAVQLPVLKDILKAMNIT
                     LIEKEGYEADDILGTLSKRASDEFDVVVLTGDRDMLQLITDKCTVKIPTVSSGQKTVN
                     VFDINTFREEYGIEPSEFIDVKALMGDKSDNIPGVQGIGKVGAFNLIKEYKDIDNLYR
                     NIDNIDKPALKEKLIKDKENVFLSKKLAKIDTEVPIDDLKPDNLEKMPFNNQQLLKLF
                     VKLEFNSFIQKFQLEEKDENISESAQHFLEVSDLKDFDTVGSRLFLYYKLDENILEIE
                     IMNEKHQILKTFDKEADNCLKEIFSSDLTVVSHYLKDIFVYCFYNNIKIPEKYYDTAT
                     AMYLLDSMKEKYDIDDTYRNHNISGSVNDPKPYKIKYLQKYSENELKTTDMQSLFYDI
                     ELPLVEILAYMEYIGIRADREYLLELSEYFDNELNITAKKIFDYAGCTFNINSPKQLG
                     IVLFEQLKLPHAKPNASGGYSTSADVLEKLRRYPIVEEVLYYRTVSKLYSTYAQGLLS
                     AIQIDGRIHSKFNQTVTATGRISSTDPNMQNIPIRTEIGRKIRKAFIPEEGYTIVSGD
                     YSQIELRILAHIADDENMRNAFLNKEDIHASTAAKVFGVNLADVTKEQRRAAKAVNFG
                     IIYGISDFSLSRDINVPVKKAAKYIEDYLNHYSGVKEYMQKIVRSAMENGYVSTIYGR
                     RRYIPELSSPKFQIREFGKRVALNAPIQGSAADIIKIAMIRVYKQLKEQNLKSRLILQ
                     VHDELVIETHKQEIGQVKEILQQSMEKGFDLSVPLDIEISVQDSLLKG"
     gene            complement(6341..7087)
                     /locus_tag="PLF14_06805"
     CDS             complement(6341..7087)
                     /locus_tag="PLF14_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71034.1"
                     /translation="MSKCGNCKKLIHEYLNSGRSQQIKEKLDLHLSVCHSCKEYFDYM
                     LAINNSLRKMKSTAVINVKDVVMESIREEQPQPVRYRKVYIGYAAAVACVAIVVGVFA
                     ITGGFRTKLYKEAAGIPNSTYRDSDEQIIEKEPAFAPRACTMANEDALGIESADEKSF
                     EDMIICNYSTDKSYNDLMKEITDLSEQFVIFNIYNQADMNEISFISNTADTEKISEEL
                     NLKKDITVKTETNTADSVSEYTKVIIIYKD"
     gene            complement(7124..7705)
                     /locus_tag="PLF14_06810"
     CDS             complement(7124..7705)
                     /locus_tag="PLF14_06810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT71035.1"
                     /translation="MEYTDEQLINMSVKGDTSAFGVLVERYQNKAYAISIKIVQNHDD
                     ALDCVQDSFIKAFRSLSSFNFQSSFNTWLYRIVTNTSLDLLRKNKKYQSNIPIEKPLS
                     GDDDDDYFIQIEDQKADVENIAESNETVKAVREAISQLSEDLKKVILLFEIEQFSLIE
                     VSEILNIPVGTVKSRLFRAREKLAILLKDKELF"
     gene            7860..9227
                     /locus_tag="PLF14_06815"
     CDS             7860..9227
                     /locus_tag="PLF14_06815"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HOT71036.1"
                     /translation="MKLKVVLSILLCKITCAVLRVFKKGGTSLPGKIAMLFCKDILKY
                     VSANVKVILVTGTNGKTTTCRIIETALENAGINYFSNKSGANLLNGVVTSFCLASSLS
                     GKNRHDYAVIECDEAALRQVTKYINPQVILVTNIFADQLDRYGDVNNILRLIADGINN
                     CDSPICINADCSLCTHLMALTKNRTRLFGVDVKMPDTHINEQNDVLHCIKCENPYTYD
                     YVTYAHLGGFRCDNCGYRRHKPDVGAVSVKNMDVTGSDIAMTVDGRLYDVRVNLPGAH
                     NIYNALAASCALNAIGIADKHILTAVSTFKSGFGRMETVKINNVPVNIILVKNTAGFN
                     RIIDYLFLVDDEYILCVILNDKPADGTDISWINDVVFEKLEEINDKIRYVYLGGSRAE
                     DMALRLKNVDFDMNKVSVAKNDATLVKTALEHNIPIYITATYTAMFNLRNMLTKKYSL
                     TEFYK"
     gene            complement(9237..11354)
                     /gene="topA"
                     /locus_tag="PLF14_06820"
     CDS             complement(9237..11354)
                     /gene="topA"
                     /locus_tag="PLF14_06820"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOT71037.1"
                     /translation="MSKNLVIVESPAKANTIKKFLGKDFTVMASFGHVRDLPEKAFGI
                     DVENHYQPNYEILSGKKEVVDKLKKAVKESDRTYLATDPDREGEAISYHLAHLLKIDP
                     QSKCRIVFNEITKPAVTNALNETKPINMDMFYAQQARRILDRIVGYKTSPLLWKKIKK
                     GLSAGRVQSVALELVCDREDEIRAFVPEEYWNLMLKLSKISHDQIFIAKFYGKDNEKV
                     KLSNETEANEVYNFVSNNIVSVKSVKKTKRKKYPFPPFITSTLQQDASNKLGFTTSKT
                     MKIAQELYEGIELKNDGTVGLITYMRTDSTRISSDFQMATRDFIKQNFGNEFVPANIP
                     SYKNRNNAQDAHEAIRPTDFNLTPTKVSASLTKDQASLYTLIYNRYIASQMSPAEYDN
                     TAVDIVCGPYLFKAVGNIVTFKGYMTVYEEHRIEQSDSEKDEYENTYLPKLVAKEQLM
                     KHDLQKIQKFTTPPSRYTEASLVKMMEEKGIGRPSTYAPTINIILKRNYVKREKRILV
                     PTDLGMTVNTFMVRNFETLLDYGFTAQMEEQLDEIETGKVDWISLIDKFYKDYIEQIY
                     IADIRTDKIDLLSDEICEKCGKPMVIKSGRYGDFLGCSGYPACNFTKKIIDDTGYKCP
                     KCNGKVLNKKTKNGVKFISCENYPKCDFSSWGTIIKDKKCPKCDNFLLKVFEDKQAMT
                     KCSSDKCDYKTKFVKVTKGKKEK"
     gene            complement(11368..12252)
                     /gene="dprA"
                     /locus_tag="PLF14_06825"
     CDS             complement(11368..12252)
                     /gene="dprA"
                     /locus_tag="PLF14_06825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOT71038.1"
                     /translation="MNEAGYIFSCIDFLSPNEKYRLYMASKSVYEIFDMNKDDLLYLS
                     KISDVPYDKLKTVRNISYPKYQKGNSDIKITDIYSEDYPQILKETYGAPIVLYHIGDI
                     PKECMVSVVGSRKPGPYGMRCAEVLSVNLSLQGFTVVSGMAKGIDGICHMAALQYGNS
                     VAVLGNGLNVCYPSENRNLYDMLIKKGCLISEFPPDSKPKKINFPMRNRIISGLSFCT
                     VVVEAGIKSGSLITANCALSQNREVYCFPNYIDTGFNGTNLLIKEGAGIITDIDDFCR
                     ELNTIEINRKTIYYCKSN"
     gene            complement(12422..14158)
                     /locus_tag="PLF14_06830"
     CDS             complement(12422..14158)
                     /locus_tag="PLF14_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOT71039.1"
                     /translation="MNLLNVTIDGIKVQVPEGTSVLDAAKLANVDIPTLCYLKDINEI
                     GACRMCLVDVGGRALQAACVYPCSEGLKVITNSPKIRDIRKATLELILSHHDRSCLTC
                     IRNQNCELQALADKLNVRDIPFDGAPKGLPIDNISKSIVRDNNKCVLCRRCTSICNNI
                     QTVGAINVMERGYETVVSTTDHRSIADVNCVNCGQCINVCPVGAIYEKDNTQAVWDAI
                     KDPDKYVVIQTAPSVRAGIGEEFGYPIGTPVTGKMVGAIKKMGFDKVFDTDTAADLTI
                     MEEGTELLTRIKTGGKLPLITSCSPGWIKFCEHEFPEFLDNLSTCKSPQQMFGALIKS
                     YYAEKNNIDPSKIFVVAVMPCTAKKFESDREEMFNDVDVSITSRELARMIREAGIDFR
                     SVEEAQFDNPLGEATGAGVIFGATGGVMEAALRTVYYILEGKRINEEALMPIRGTEGI
                     KEATLKVAGMDVNVCAASGLGNARKVLNMVKNGEKNYHFIEIMACPGGCVNGGGQPIV
                     PASIRNKIDIRAERAKALYSEDKRATYRNSDENPFIQKFYKEYAGEPGSHKAHELLHT
                     HYQKREKHLKNK"
     gene            complement(14171..16009)
                     /locus_tag="PLF14_06835"
     CDS             complement(14171..16009)
                     /locus_tag="PLF14_06835"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOT71040.1"
                     /translation="MKSLTELKAIKQKVTEVPDYKIVVGMATCGIAAGANPVFKELKR
                     IADEKKLSNVDIQITGCIGICRLEPIVEVYTAKGERYTYVKVDAEKAQDIIQKHIIEG
                     KPCTDYLICDDGEACIKNIQETKSFKKQIRIALRNCGNINPENIDEYIKNDGYFALGK
                     VLTEMKPAEVIQLLLDSGLRGRGGAGFPTGLKWKFAAAAQNEQKYVCCNADEGDPGAF
                     MDRSVLEGDPHSILEAMAIAGYAIGSDQGFIYVRAEYPVAVKRLNLAIKQAKEYGLLG
                     NNIFGTDFSFDIELRLGAGAFVCGEETALMTSIEGKRGEPRPRPPFPANKGLFGKPTI
                     LNNVETFANIPVIINKGADWFNKIGTEKSKGTKVFAVGGKINNTGLIEIPMGMTLREV
                     IYEIGGGIPNGKAFKAAQTGGPSGGCIPAEHLDVAIDYDNLIAIGSMMGSGGLIVMDE
                     DNCMVDIAHFFLGFTVDESCGKCPPCRIGTHRMLEMLEKITAGKGEDKDIEKLERLAY
                     SIKEAALCGLGQTAPNPVLSTLRYFKDEYKAHIYQKTCPAGVCKNLLSYNIDAEKCIG
                     CGLCARNCPANAITGVVKSPYTIDKDKCIKCGVCMEKCKFGAIYKK"
     gene            complement(16031..16504)
                     /gene="nuoE"
                     /locus_tag="PLF14_06840"
     CDS             complement(16031..16504)
                     /gene="nuoE"
                     /locus_tag="PLF14_06840"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013432578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOT71041.1"
                     /translation="MCQNCNLDENQKKLQEVFDKYKDTQGALIPVLHEAQEIYGYLPL
                     SVQEAVADALEIPLSEVYGVVTFYTHFSLYPKGKYKIQICLGTACYVKGANNILEELK
                     KQLGIDVGQSTKDGKFSLDACRCVGACGIAPVMMINDDVYGSLAVSDVADILKKY"
     gene            complement(16670..17785)
                     /gene="nagA"
                     /locus_tag="PLF14_06845"
     CDS             complement(16670..17785)
                     /gene="nagA"
                     /locus_tag="PLF14_06845"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HOT71042.1"
                     /translation="MILSNCVVFDGVDFKEGLQNIYIENALITDIRTYTHEPHSININ
                     GCIAVPGFIDIHTHGICGTDFSYSFDADADKFFDNYVKVGTTSVFPTTVTMDPDKLNL
                     LLSKYSKIKHPAFCGIHLEGPFINIKKAGAHNKQYIMQPSVESYRKITGQYENIVKRV
                     TIACELDQGYKLAGYLKEHNKVVSFGHTECISDEAYKAFKAGYRLSTHHFNAMPLMHH
                     REITVTGAALVDDCVSCEYIPDFYHVGKEMLKILFKCKKPENLVMVTDSMSATGMPDG
                     EYTLGDSDVFVKDGLVKNQSGTIAGSSVTMAKGVFNMINAGFNPVTVLKSATSVPAKV
                     MNLKKRGILRPGYYADINILDSKFRYMFTISKGTRID"
     gene            complement(17797..19809)
                     /gene="recG"
                     /locus_tag="PLF14_06850"
     CDS             complement(17797..19809)
                     /gene="recG"
                     /locus_tag="PLF14_06850"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008165.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOT71043.1"
                     /translation="MNELAALKGIGPKIIEKLNTLSIYNTNDLLEYYPRTYVDKTGIS
                     HISDIREGEYFSLKGILYNITSKQLPYGRRLHIVTAEFKDQTGQISVTWFNQPYLKNV
                     LKENKMIYIYGKVEKKGFFYNMYNPDFSYNEKDFFIIQPIYSKNAKINQYEIRKAVKS
                     AMDYSHVLVKDYFPEYLKERYNLCDKEFAVKNIHFPASSADFFAARRRLVFEEFFLMQ
                     LALVSLKSNMGKDKSPINISKFDMAREFVRSLPFTPTDAQLQSMKDIASDLSSGYSMH
                     RLLQGDVGSGKTVVGLYAVMCAYRNDYQSAFMAPTEILALQHYRTAVELLNKFGLNIA
                     LLTGQLRKKEREDILNRIENGKVHLVIGTHAILQEDVIFHNLALCITDEQHRFGVQQR
                     LILSSKGVKPHLLVMSATPIPRTLALILYGDLDISTLKELPKNRKKVLTYSVDESFRQ
                     RINNFIQKIVVKGEQAFVVCPAIYENDVLDVTNVLTKYEQLQTDIPSVKSAVLHGKMK
                     KVEKNEVMEKFINNEISVLVTTSIIEVGIDIPNATLMVIEDAQQFGLSQLHQLRGRVG
                     RSDKQSYCILFNQSSSDISKKRMDVMVQSNDGFYISEQDLLLRGPGDFFGTKQHGLPD
                     FKIANLYTDMDILKDAQKAVQEEIRNNAIYRQLLGRELDYLSKAKL"
     gene            complement(19802..20545)
                     /locus_tag="PLF14_06855"
     CDS             complement(19802..20545)
                     /locus_tag="PLF14_06855"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HOT71044.1"
                     /translation="MIDNLVKEIKKAKSIVFFTGAGVSTESGIPDFRSTKGLYLSKYD
                     GYTPEVIISHSFFYHNPDVFYRFYHDKIVHPEAKPNDFHYGITKFKDMNYDVSVVTQN
                     IDDLHNIAGNGKVYELHGSISRNNCIKCHKFFNLDYIIANRGKTVRCDECNSLVKPDV
                     VLYEEQLDQVIIEQSIQAIMNADVLIVAGTSLSVYPAASFIDFYTKNCLCILNKTSTP
                     RDSKADYVFYDSCSVVLNKIIEKLERNNE"
     gene            complement(20545..21414)
                     /locus_tag="PLF14_06860"
     CDS             complement(20545..21414)
                     /locus_tag="PLF14_06860"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HOT71045.1"
                     /translation="MIKLKNRHLTVIINPLGAEIVSVIKDGYEYLWQGNPDIWKGQAP
                     NLFPFIGRLKDEEYEYDNRLFKVKIHGFARHSLFEIVESNENYVSLRITGTPDTYAVY
                     PFDFDFIVSYELMDNIIKKQYMIINNGYKVMYYEVGGHEGYNLTFEKNKIMDDYYILF
                     SDNIIYNYTTDKNIMINKQTEEIKLSDRKLYLKPEIFKNDALILDCSKLSNREVVLKC
                     STSSRKVKVRFEDFDYLGIWTHPFYTNYVCIEPWSALPDCNFIDKKLENKIGIRKLTA
                     KKTDMLEYFIDVL"
     gene            21500..21727
                     /locus_tag="PLF14_06865"
     CDS             21500..21727
                     /locus_tag="PLF14_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOT71046.1"
                     /translation="MKNKIDIKFLSISDVKEFADIVSAFDYDIDLVSGRYVVDAKSIM
                     GIFSLDLSKPISLVAHTDDIKPLTDAIKRFC"
     gene            complement(21853..22662)
                     /locus_tag="PLF14_06870"
     CDS             complement(21853..22662)
                     /locus_tag="PLF14_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOT71047.1"
                     /translation="MTQCNHNCSDCSQNDCESRIQKEQTNDFSQIKNVVAVLSGKGGV
                     GKSMVTSLLAVELTRAGYKVGILDADITGPSIPKAFNLSEHIRGNETGIFPAKTNFGT
                     KIISINLLLENTTDPVLWRGPVISGVLKQFYTDVVWGELDYLLIDMPPGTADTALTVF
                     QSIPVDQAIIVTTPQDLVSMIVEKAIKMADMMHISVLGAIQNFSYAVCDKCNNKIELF
                     GDTDTNKLSIPVVDRIPFDKRLSAAMDRGMIELTEDTYIANTVKKLIEKSK"
     gene            complement(22674..23666)
                     /locus_tag="PLF14_06875"
     CDS             complement(22674..23666)
                     /locus_tag="PLF14_06875"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT71048.1"
                     /translation="MKNRRILSIILVVLTVLSLTGCRKVYETHRPVKPVTANIVALKG
                     PTGIGMVKVMNDIISLSDYVNTKFKIMTSPDTVVAGLLDKSITIAAVPTNLAAVLYNK
                     TEGEIQILAVHTLGVLHILDKTGNIKSIKDLKGKTLVTSGQGSLPQYVLNFILSENGI
                     NPETDMEIVYKAEHSEVATLLLSGEADVVMLPEPFVSTVLNKDASINHAINLNDEWVK
                     SAPDITLSMGCLVAQKSFIEEYKEEVDTFLELYEESIDWVLEQPYNAAPLIVSSGILD
                     NEKVAESAIPNCNIVYIDAADAKDSLNAFFKFLYNNNPASVGGKVPDNGIYYER"
     gene            complement(23736..25736)
                     /gene="ligA"
                     /locus_tag="PLF14_06880"
     CDS             complement(23736..25736)
                     /gene="ligA"
                     /locus_tag="PLF14_06880"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOT71049.1"
                     /translation="MYMKNRIDELVKILNYHNKRYYELDAPEISDYEYDMLMNELIAL
                     EQQYPEYRSVESPTQRVGGKPLKEFAKVKHTVPLESLSNAFSYEELYAFHERILKESI
                     NPEYVLEKKIDGLSVTLEYADNIFVRGATRGDGIYGEDITENLKTVHNIPLKLHTKEV
                     IPYLVVRGEVYMDKDNFAKINEVAEENGEKPFANPRNAAAGSLRQLDSKITAKRNLKI
                     FVFNLQSIQGALFDTHVQTLNFLKDAGFVVNDVIRVSGDMKDIISGIEEIHDKRFSYK
                     YDIDGAVIKLNNIADREVLGSTAKAPRWAIAYKYPPEQKETVVKDIFVQIGKTGVLTP
                     NAEFDPVFVSGSTVSRATLHNMDFIDTNDIRINDHVIIQKAGDIIPEVVRVLKDKRTG
                     KEIKFKMPENCPFCNSDVQRVKDQAAYRCTNINCTGQISRRLEHFCSKDAMDIEGLST
                     ATVEKFMDLSLLKDIADIYDLHNKREQLLKIEGFGEKSVNKLLSAIEKSKSNNIDRLL
                     FGIGILYIGQKASSLLAENFPDMKSIMAAEITDFTSIDTFGEVMANSIVDYFKDDKAV
                     ELIKRLEAQGVNMKSLSYNNTQKLSDKLIGKTYVITGSFEEYTRDQLKSIITSNGGNV
                     TESVSKKTDYVLVGDKPGSKLTKAQALGINIIDLEQFKFSLL"
     gene            25823..26074
                     /gene="rpsT"
                     /locus_tag="PLF14_06885"
     CDS             25823..26074
                     /gene="rpsT"
                     /locus_tag="PLF14_06885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOT71050.1"
                     /translation="MANKKSAEKRVSVSMKKRLVNSAKKSSTRKQLRDARLAIENDSP
                     DKKQLIMEAMKAVDKAASDGIIHRNKAARHKSKLSVHLK"
     gene            complement(26179..27300)
                     /locus_tag="PLF14_06890"
     CDS             complement(26179..27300)
                     /locus_tag="PLF14_06890"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT71051.1"
                     /translation="MRWDRKEYLDYVTFKENKRPMFVELFGPLIGLEEEWIEQGATED
                     EINMTAFDWDYVPYVNCGANCDIFDGPEERVIEETKEHIITIDALGRKMKLMKGAATI
                     PLPMDYPVKNMDDWLKLKHLYQFREERIDYNQVENAKEQQKQGVMVRAEVPGGFDLPR
                     QLMGEEELCYAYYEQPELIEDILNTAADTAIKCCERLSKHLIVDHLHIHEDMAGKSGS
                     LIGPVHIEEFLQPYYLKIWDAYKNNGCTIFVQDSDGNMNSVIDAFLNCGLNQMYPMEP
                     AAGMDIVKVHAKYGTRLSLKGGIDKFILRDSKLAVLKELEYKMQPSMLKGGIVFGLDH
                     RIPNGSSLELYRYYVKTAREILGREPLDKKNKGWMRQAI"
     gene            complement(27336..28295)
                     /locus_tag="PLF14_06895"
     CDS             complement(27336..28295)
                     /locus_tag="PLF14_06895"
                     /inference="COORDINATES: protein motif:HMM:NF017911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71052.1"
                     /translation="MKYEDIKNEISGNIFKQVYLFYGDDEYLKQSVVNALKTAIIRYT
                     QAEYDNVKFDDTSKIEDIINECATYSFSGNNKFITCRNTGFFNKEEFSDKIISLSDYV
                     TEGVYLFFIENDINKRLSGFKHYNSKNSAYEITKGTAEDIKRFVYSAFRKEGKNISSE
                     NLNLFLEYSGLNLSFVTLSIDKILLYIQPSNEVTAETIKLLCTGITDVKGYELTNNLC
                     KRDLSAALDVYYDMVSLKYALPYFTAILFNTFYEMYTIIVNNITSSNDFRVRKAIQNS
                     KEFSIEQLKNIIYAISEADHDFKSGKLGQDSAMVTLMSTIVNS"
     gene            complement(28315..>28873)
                     /locus_tag="PLF14_06900"
     CDS             complement(28315..>28873)
                     /locus_tag="PLF14_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71053.1"
                     /translation="GAIEDVLDNFSVDNVYLPYTNHKDNIKLSDQLSMNYPDTYCGML
                     KRGDEIRTEGVCIKVLNPDTDIVYTNANDGSVVFNLYTKAGSFLFTGDADIKKIPSDI
                     FLKCDIIKMPHHGDDKAINSEIIHYISPKIAVISVGKHNRYGHPGRKTLDTLAEKGIN
                     TLRTDINGSVTVYLINRKYYLFKYR"
BASE COUNT         8149 a         5999 c         4904 g         9821 t
ORIGIN      
        1 gagaaaaact ctctttgttc ttcaccttca ctgagactta tctttttgat atcttcacac
       61 atgatcggtt ttttatcttt tataagaggt tcaaaaaccg gaaaatccgc cattgtgact
      121 gtcattccgg gagtcaaggg aaatgattcc gcatccggtt cgtttacata tgtgtcaaaa
      181 atcttcgcat cctgataatc attatcgaat tccactaaat atgcgtgatt atagttgaga
      241 acttccagtg acattttaaa catatcctgt attttttcca tcacatttcc actgttgacg
      301 gaaataaaac tggtggatat cttttccaga accaattgtt cctttgcaaa tcttttatac
      361 tcattttcct ttgatgagta ctctcttgtt acatatctga tactgaaaaa ggaaagaaga
      421 ataattccga ttcttaccgc gtactgactt ccatcaataa ctacgacaac ttgcggataa
      481 acaatccata ataaaacttg aatcataata acgaatatcg tataaataag agcatgaata
      541 cgtgtgccca gaacaaccgt gaataagaga aagataatat aaacaaacca tgttgtcaaa
      601 ccaccttcat cagcggatgt tgtgctgtat aatgtcatac cgaacataca gaccagcaga
      661 aagagtacat tctgaacgga atgctttttt atcatgtaag gtataaattt agaaactaat
      721 gcgattgcaa aaattgaggc ggagagaaaa atctccatat ccaacggctt tttcataaag
      781 aaatatccga taaggaatga aacaaaggaa ccgattaaaa atattattga agtggtttca
      841 aataaccgta aacgactgct ttgtccttct atttctcctt catgaaaaac ataaaccgtc
      901 ttctcccttt catgaaagat tccgtatttt ttcaatgcta taaacaaggt aattgtcggt
      961 agcatgttga agaatatgaa tgttttgaga agaagatatc tcaacggaat gttggggagt
     1021 atatttgccg ttattcctac taccgaaggg agtactgttg ataaaaataa gaaattcaga
     1081 tggcgtttaa taaaatcatt gggcctgact ttcttccacc aggaaagcag taaaaaaact
     1141 cctacaacgg gataaattat aaaatataaa accagccaga ttctggaaaa atttgcagga
     1201 accgcgttta tccaaccgaa ctcacttttt gtcatttggt tctgcatctg attaagaatt
     1261 ccgaaaggag aaaaaagtac gatgtttaga aatgccggag tgtaaattaa aaaatgcgtt
     1321 ctgggcttat ctaaaaatgc tctgttattt gtgataacaa taataaagtg aataaggata
     1381 gcataaaata caccccaacc gaaaaccgag aaggctctcc aaaaagcact ggattccgct
     1441 gaaggagccg agttgacaat ggaatcacaa taagcccata tggctaatgt cgcggtcaga
     1501 taaaaaaaca ttcggttaac tttacttttt atattcgcat aaattgcgta tattccgaag
     1561 aacaggtaaa aacacccgca tagataaagt gataccgata aaatcaaggc ataatccatg
     1621 tcttattcct caaacacgcc caaaaagcaa tcaaaggacg aactttcttt aataacatat
     1681 accattaata ggataaaata tcaatatata tattttaaaa attttaaaat tttatcaaaa
     1741 tttgcaaatt ttaatatgaa tattacttac ggcaccggtg ttataggaat ctttaactct
     1801 tgccctgcat aaatcatatt tatatcttta atattattca aacccgcaat cacttctaca
     1861 aatattgcag tattaggata attcagactg tatacatcgt tatagtaatt ggctatctga
     1921 ttcaggctgg tatggtcggt tgtaaccttt actatatgag taatatactg aggctctatt
     1981 gtaggttcgg gtgattcggt aggctcttcc gaaggagtag gtgtcggagt cggagtggga
     2041 gtaggtgtct gtaccactat cggtgatgtc acggctacag gtgaggtttc aacaggtttt
     2101 tttctgttaa atatcagttt aagcagattt accattccga taataatacc tgccataacc
     2161 gccagaacaa caacaatact tattgctctt tttatatata tggaagttct tctcttcctg
     2221 ctttcacttt tactcagttt aaccgaatca aaggaagaaa ttttttgcat ttttgtcttt
     2281 ttgtcggtaa atacaatgtc tttggatgaa tcatcccaga agctttcttt cttctcaggc
     2341 ttatcggttt tatcctcata tatcagaggc tgagttgatg tataggtttt ttgcagctct
     2401 tccgtcggta tctcctgagt atttatatca cttacagcgg atactttaga cttatcctga
     2461 ataagtttta ccagtattac tgttactgaa aactcttcat tattgaatga agtataaagt
     2521 gagcaaatat tctgagcaat ggattcggcc gaatcatatg tatcaatacg ccctaaaata
     2581 tcggctgcgg agagggcctt tgctaccgtt tccgataata atataatcag gtcattggtt
     2641 ttcagtacgg gaatgggtgc cgtttttatt ctgctgtcat cgtcttccgg aaacactctg
     2701 actatatttc ctccggatat atatattgcc gtatcggtat cggtaaccgc cagcagacct
     2761 ttatcatgtg aaataagggc ggcactcacc tgtctgttca tggtatctat ttcacctgtt
     2821 tcttcaagat tgtccagata agataccgtg gtggagatat attcttcaaa atcggaaaac
     2881 ttggtttccg tctcatactc tttatatttc ttagcgtatc ttcttatctc gcgcatcagt
     2941 gatacatcat tgcccgtatc gtttgtttcc atattctgtt cgattagaaa tataaaatcg
     3001 gtatcggcat actccattat gacttccttg ctgtcataat tcagagtatt gataaccttg
     3061 ccgttaacat atgctttaag atcggtttct tttttgttac tacaagtaat atttgcggta
     3121 ttgagtaata ttttagccat atttccacct ttctttttaa ttcgaataga attttaacat
     3181 tcgatgtatt aaaaatcaat ccttattgac ttttatgtaa gtattttcaa agctatctgt
     3241 aaatgtcatc aaatcgcttt tatcggtttt atcggtattt aatattatat cgccgattga
     3301 gagatagtct tctcttccct tctgcatttt tatcctctta caggcttgtt cttcatttat
     3361 gccatatcgt tgcatcaaac gcgataactg aaccttcttt gaagcaacgg ttacgataat
     3421 aatatcggtt atatcgataa agcctttttc cacgggaacg ggtacatcca gaacgatgtt
     3481 tttttcggaa ttttcttcaa caaattcata tattttctcc gctacatact tatgtgtaat
     3541 tgcattaagt ctttccagtg attctttatc atcaataact cttgaaagaa taacctttct
     3601 gtcaagagtg ttgaactcgt tgagtaattc tttctgcatg caaacggaag tatccagaag
     3661 atgcttatag gtttcatcgg catcaaaaac cgcatagcct ctttgggcaa atgcttttgt
     3721 cagcgatgtc ttacctgtgg cagtctgccc tgtgattcct ataatcatct ttctatcctt
     3781 tcaaaagaga atcctgaacg gatatttcta tgtcgagagg tacagataaa tcaaatcctt
     3841 tttccatgga ttgttgtaaa atctctttta cctgcccgat ttcctgttta tgtgtttcaa
     3901 taaccaattc gtcatgcacc tgaagtataa gacgggattt caggttttgc tctttcagtt
     3961 gcttatatac tcttatcatt gcaatcttta ttatatccgc agccgaaccc tgtatgggtg
     4021 catttaaagc cactctcttg ccgaactccc ttatctgaaa cttaggtgaa ctgagttccg
     4081 gtatatatcg tcttcgtccg tatattgtgg aaacatatcc gttttccata gccgaacgga
     4141 ctattttttg catgtactct tttactcccg aataatgatt gagataatct tctatgtatt
     4201 tagcggcttt tttaaccggt acattgatat ccctggataa actgaaatcg gatattccgt
     4261 aaattattcc gaagttaacg gctttggcgg cacgtctttg ctcttttgta acatcggcta
     4321 aattcactcc gaacaccttt gcggcagtgg aagcatgaat atcctctttg ttaagaaatg
     4381 catttctcat attctcatca tcggcaatat gtgcaagtat acgcaattct atctgagaat
     4441 aatcaccgct tactatcgta tacccttctt ccggtataaa ggctttacgt atttttcttc
     4501 cgatttccgt tcttataggt atattctgca tattgggatc ggttgaactt attctcccgg
     4561 ttgcggttac cgtttgattg aatttactgt gtattcttcc gtcaatctga atggcggata
     4621 acagaccctg tgcataagtg gagtaaagtt tcgaaacggt acgataataa agtacttctt
     4681 ctacaatggg atatcgcctg agtttctcca aaacatcggc ggatgtggaa tagccgcccg
     4741 atgcattggg tttggcatgc ggtaacttca attgttcaaa taatacaata cccagttgtt
     4801 tgggagaatt tatgttgaat gtacaaccgg cataatcaaa tatcttcttt gccgttatat
     4861 tcaattcatt atcgaaatat tccgataatt ccagtaagta ctctctgtcc gcccgtattc
     4921 cgatatattc catatatgcc aatatctcca ccaaaggcag ttctatatca taaaacagcg
     4981 attgcatatc cgtagttttt aactcatttt cggaatactt ttgaagatac tttatcttat
     5041 acggtttggg atcattgacc gagccggata tattatggtt tctgtaggta tcgtcgatat
     5101 catacttctc tttcattgaa tcgagaagat acattgcggt ggccgtgtca tagtattttt
     5161 ccggtatttt aatattattg taaaaacaat aaacgaaaat gtctttgaga taatgagaaa
     5221 caacagtcaa atccgatgag aaaatttcct tgaggcagtt gtccgcttct ttatcaaaag
     5281 tctttaaaat ctgatgcttt tcgttcataa tctctatttc taaaatgttt tcatcaagtt
     5341 tatagtacag aaataatctt gaacctacag tatcaaaatc ctttaagtcg gaaacttcaa
     5401 gaaaatgttg tgcagattca cttatattct catctttttc ttccagctga aacttctgaa
     5461 taaatgaatt gaattccagt tttacaaaca acttgagaag ttgctgattg ttgaaaggca
     5521 ttttttcaag gttatccggt ttcaaatcat caataggtac ttctgtatct attttcgcaa
     5581 gttttttact caaaaagaca ttttccttat ctttgataag tttttccttc aaagcgggct
     5641 tgtctatatt gtcgatgtta cgatacaagt tatcgatatc tttatactct ttgataagat
     5701 taaaagcacc tactttgcct ataccctgaa ctccgggaat attatccgat ttatcaccca
     5761 tcaacgcttt gacatcgata aattccgaag gctcgatacc gtactcctca cgaaaagtgt
     5821 taatatcaaa cacattaacg gtcttttgtc cggatgagac ggtaggaatt tttaccgtgc
     5881 atttatccgt aataagttgc aacatatccc tgtcacctgt aagtacaacc acatcaaact
     5941 catcggatgc acgttttgac agtgttccca gtatgtcatc ggcctcatat ccttctttct
     6001 cgattaaagt gatattcatg gctttcagaa tgtctttcag taccggcaac tgtacggcca
     6061 attcgttcgg cattcctttt cttccggctt tgtattgctc atatatttca tgtctgaatg
     6121 ttttaccctt caggtcgaaa gcaaccgcaa tatgagtggg attatcttca ttcagatatt
     6181 tttccagaat attgagaaat ccgtatatcg cgttggtaaa taaaccgtct ttggtaacca
     6241 gcatattgga tgccataccg tagaatgctc tgttcagaat actgtttccg tcaattaaaa
     6301 gtagtttttt catcggattc aattatatca tggattatgc tcagtcttta tatattatga
     6361 taacttttgt atattcgctg acactatcgg ctgtatttgt ttcggttttt acagtgatat
     6421 cttttttcag attcaattct tcggaaatct tttcggtatc cgcagtattg gatatgaaag
     6481 atatctcatt catatccgcc tgattatata tattgaaaat aacgaattgc tccgataaat
     6541 cggttatctc tttcataagg tcgttataac ttttatcggt agaatagttg catataatca
     6601 tatcttcaaa gcttttttca tctgcggatt caattcccag tgcatcctca ttcgccatag
     6661 tacaggctct gggggcaaaa gcaggttctt tttctattat ctgttcgtca gaatctctgt
     6721 aagttgaatt cgggattccc gcagcttctt tatacagttt ggtgcgaaaa cctccggtaa
     6781 tagcaaaaac ccctacaacg attgcaacac aggcaaccgc tgccgcatat cctatgtata
     6841 cctttctgta tctgacaggt tgaggctgct cttcccttat actctccata acgacatctt
     6901 taacattgat aacagccgta cttttcattt ttctcagcga gttgttaatt gcaagcatat
     6961 aatcaaaata ctctttgcag gaatgacaga cggaaagatg aagatccagt ttttccttga
     7021 tttgctgcga ccttccgctg ttcagatatt catgtattaa ttttttgcaa ttcccgcatt
     7081 tactcattat taacaaatcc tcatatatat aacgtagcac acctcaaaaa agttccttat
     7141 ctttcaataa tattgcaagt ttttcccgcg ctctgaataa tctggatttt accgttccga
     7201 caggaatgtt aagaatctcg gaaacttcta taagtgagaa ctgttctatc tcaaacaata
     7261 atataacctt ctttaaatct tcagacagtt gactgatggc ttctctgacc gcttttaccg
     7321 tttcgttgct ttccgctata ttttccacat ccgccttttg gtcttctatc tggataaaat
     7381 aatcatcatc gtcatctccc gataaaggtt tttctatggg aatattgctc tgatatttct
     7441 tgttcttacg cagaaggtcc aaagaggtat tggttacaat cctgtataac catgtattga
     7501 atgacgactg gaaattaaaa gagcttaaac ttctgaatgc ttttataaac gagtcctgta
     7561 cgcaatccaa agcatcatca tgattttgca caatcttaat ggatattgca tatgctttgt
     7621 tctgatagcg ctcaacaaga acaccgaaag ccgaagtatc gccttttacg ctcatattta
     7681 tcagctgttc gtcagtatat tccatgaaac ctccgtaggt ataatataac atgaaaatat
     7741 gtttatttgc attatattat ttaatccttt tatcggcatt aattgacaac cacttttttc
     7801 tttaagtcta aagaggattg tttattgata atcctctttt gcgctgatat aattttttta
     7861 tgaaattgaa agtggtatta tcgatattat tatgtaaaat aacctgtgcc gttctgagag
     7921 tttttaaaaa aggaggcact tcccttcccg gcaaaatagc aatgctgttt tgtaaagaca
     7981 ttcttaagta tgtttccgca aacgttaaag ttattcttgt aaccggcacc aacggaaaaa
     8041 caaccacctg cagaattata gagaccgctc tggaaaatgc gggaattaat tatttttcca
     8101 ataaatcggg agctaacctg cttaacggag ttgttacctc cttttgcctg gcttcttcgc
     8161 tgtccggaaa aaaccgtcat gattacgccg tgatagagtg tgatgaagca gcgctgagac
     8221 aggtcaccaa gtatataaat ccgcaagtta tactggtaac caatatattc gccgaccagt
     8281 tggacagata cggtgatgta aataatatat tacggttgat agcggacggc attaataact
     8341 gtgattcgcc gatatgtata aatgccgatt gttcgttatg tacccatctt atggctttaa
     8401 caaaaaacag gacaaggtta ttcggtgtcg atgtaaaaat gcctgataca catattaatg
     8461 aacagaatga cgttttacac tgtattaagt gcgaaaaccc ctatacctat gattatgtca
     8521 catatgctca tttaggcggt ttcagatgcg ataactgcgg ttatagacgt cataagcctg
     8581 atgtcggtgc cgtttctgta aagaatatgg acgtaaccgg aagcgatatc gctatgactg
     8641 tagacggtag attatatgat gtccgcgtta accttccggg agcacataat atatacaatg
     8701 ccttggccgc atcctgtgca ttgaatgcga taggaattgc agacaaacac attctgactg
     8761 ccgtcagtac gtttaaaagc ggtttcggac gcatggaaac ggttaaaatc aataatgtac
     8821 ctgttaacat cattctcgta aaaaatactg cgggtttcaa ccggattatc gattacctgt
     8881 ttttagtaga tgacgaatat attctgtgtg ttatccttaa cgacaaacct gccgacggta
     8941 ccgatatttc ctggattaat gatgttgtat ttgaaaagct cgaggaaata aatgataaaa
     9001 tacgatatgt ctatctggga ggctcaagag cggaagatat ggctttaaga cttaaaaatg
     9061 tcgattttga catgaataag gtatctgtag caaaaaacga tgcaaccctc gtaaaaaccg
     9121 ctctggagca caatataccg atatatataa ccgctacata taccgctatg ttcaatctga
     9181 gaaatatgct taccaagaaa tattcactga cggaatttta taagtaaata tttaatttac
     9241 ttttcttttt taccttttgt aaccttaaca aacttggttt tatagtcgca tttatccgaa
     9301 gaacattttg tcatagcctg tttgtcttca aatactttca aaaggaaatt atcgcattta
     9361 ggacattttt tatcttttat tatggttccc catgatgaaa aatcacactt gggataattt
     9421 tcgcaggaaa taaacttaac gccgttcttt gtctttttat tcagtacttt accgttacat
     9481 ttagggcact tataccccgt atcatcaatt atcttcttgg taaaattaca tgccgggtaa
     9541 ccggagcatc ccagaaaatc tccgtatctt cccgatttga taaccatggg ttttccgcat
     9601 ttttcacata tctcgtcgga aagcaaatct attttatcgg ttcgtatatc ggctatataa
     9661 atctgttcga tatagtcctt ataaaattta tctattaagg atatccagtc aacctttccc
     9721 gtttctatct catccagctg ctcttccatc tgagctgtaa acccgtaatc cagcagggtt
     9781 tcgaaattcc tgaccatgaa tgtatttacc gtcataccca gatctgtcgg aactaatatt
     9841 cttttttctc ttttcacata atttcttttt aaaatgatat taattgtcgg agcataggtg
     9901 ctgggtcttc ctatcccctt ttcttccatc atcttgacca gcgatgcttc cgtgtatctt
     9961 gaaggaggag tggtaaattt ctgtattttc tgtaagtcgt gtttcataag ctgttctttt
    10021 gcaacaagtt tcggcagata tgtattttca tattcatctt tctccgaatc gctctgttct
    10081 atcctatgtt cttcgtaaac ggtcatatag cctttaaagg tcacaatatt accgacggct
    10141 ttaaacagat aaggtccgca aacaatatca acggctgtat tgtcatactc cgcaggcgac
    10201 atctgactgg caatatacct gttgtatatc aatgtgtata atgaagcctg gtcttttgtg
    10261 aggcttgccg ataccttggt aggtgtcaag ttgaaatccg taggacgtat ggcttcatgt
    10321 gcatcctgag cattattgcg atttttgtaa ctgggtatat ttgcgggtac gaattcgttg
    10381 ccgaaattct gttttataaa atcacgtgta gccatctgaa aatcggagga aatacgtgtg
    10441 gaatcggttc tcatgtaggt tataagtccc acggtaccgt cattcttcaa ttctatacct
    10501 tcataaagtt cctgagcaat tttcattgtt ttggatgttg tgaaacccaa tttattggaa
    10561 gcatcctgct gcaaggtaga ggttataaaa ggcggaaaag gatatttctt tcttttggtc
    10621 ttctttaccg attttacgga aacaatgtta ttacttacaa agttatatac ctcgttcgcc
    10681 tcggtttcat ttgacagttt aactttctca ttatcctttc catagaattt cgcaatgaaa
    10741 atctggtcat gggaaatttt actgagtttc agcataagat tccagtattc ttcgggtaca
    10801 aaagctctaa tctcatcttc cctgtcgcat accagttcca atgccaccga ctgcactctg
    10861 ccggcactta agcccttttt aatcttcttc cataacaggg gactggtttt gtaacctact
    10921 attctgtcta aaatccttct ggcttgctgt gcatagaaca tatccatatt tataggtttt
    10981 gtttcattca aagcattggt tacggcgggt tttgttatct cgttaaaaac aattctgcac
    11041 tttgactgag gatctatttt gagtaaatga gccagatgat aggaaattgc ttcaccctca
    11101 cggtccgggt cggttgcaag ataagtcctg tcgctttcct ttacggcttt tttaagtttg
    11161 tctacaactt ctttctttcc gcttaaaatt tcatagttgg gttggtaatg gttttcaaca
    11221 tcaattccaa atgctttttc cggtaaatcc cgcacatgac cgaaactggc catgacggta
    11281 aaatcctttc ccaaaaactt tttgattgta ttggccttgg caggcgattc cactattacc
    11341 agattttttg acataatttc ctccaaatca attgctttta caataataaa tggtttttcg
    11401 attaatttca atagtattta attcacgaca gaaatcatct atgtccgtaa ttatgcccgc
    11461 tccttcctta atcaataagt ttgttccgtt gaaacccgta tcgatataat tgggaaaaca
    11521 atatacttcc ctgttctgtg acaatgcgca atttgcagtt atcaatgaac cgctttttat
    11581 gccggcttcc accacaaccg tacaaaatga taagccggat attattctgt ttcgcatggg
    11641 aaaattaatc tttttcggtt tactgtccgg agggaattca cttataaggc atcctttttt
    11701 aatcagcata tcatataaat ttcggttttc cgaggggtaa catacattta agccgttacc
    11761 caataccgcg acggaattac cgtattgtaa agcggccata tggcagatgc cgtcaatacc
    11821 ttttgccata ccgctcacta cggtaaagcc ttgtaaagac aagttaaccg ataatacttc
    11881 ggcacatctc attccgtaag gaccgggttt tctggagccc acaaccgaaa ccatacattc
    11941 tttcggtata tctcctatgt gatataagac tatgggagca ccatatgtct ctttaagtat
    12001 ttgaggatag tcttcagaat atatatccgt aatctttata tcactattcc ctttttgata
    12061 ctttggataa cttatattac gaacggtttt gagtttatca taaggaacat cggaaatctt
    12121 tgagagataa agcaaatcat ctttattcat atcaaatatt tcatataccg atttactcgc
    12181 catatacagg cgatattttt cgttgggaga taaaaagtca atacatgaga agatatatcc
    12241 tgcttcattc attacctgta ttttaatgta tatatttgtc ataatgaaca gacaatattg
    12301 attatattga tatttttaat tattaaataa ttcactgata tttattgttc tatacgaaaa
    12361 agcggtatta ctaccgcttt ttgcttattc ttcaaatatt aaagattacc ggtatgagaa
    12421 actatttatt cttcaagtgt ttctctctct tttggtaatg tgtatggaga agctcatgtg
    12481 ctttatgact tcccggttct ccggcgtatt ctttatagaa cttctgaatg aacgggtttt
    12541 catctgagtt cctgtatgtg gcacgcttgt cttccgagta taacgctttg gctctttccg
    12601 ctcttatgtc aatcttattc cttatcgaag cgggaacaat aggctgaccg ccgccgttta
    12661 cgcatcctcc cgggcaagcc attatctcta tgaaatgata attcttttca ccgttcttta
    12721 ccatgttaag aacttttctt gcattaccca gaccgcttgc tgcacaaaca tttacatcca
    12781 tacctgcaac tttcaatgtt gcttctttga taccttctgt tcctctgata ggcatcagag
    12841 cttcttcatt gattctctta ccttccagta tgtaatatac cgttcttaat gcggcttcca
    12901 taactccgcc ggttgcaccg aatattacgc ctgcgccggt tgcttctcca agaggattat
    12961 caaactgcgc ttcttctacc gaacggaagt ctatacccgc ttcacgaatc attctggcca
    13021 attctctgga tgtaatggaa acatccacgt cgttgaacat ttcttctctg tcagattcga
    13081 attttttagc ggtacacggc attacggcca ctacaaaaat ctttgaagga tcaatattat
    13141 tcttttcggc gtaatatgat ttgattaatg caccgaacat ctgctgaggt gatttacatg
    13201 tggataagtt atccaggaat tcgggaaatt cgtgttcgca gaactttatc cagccggggc
    13261 tgcacgatgt gataagcggt aattttccac cggtttttat cctggtcagc aactccgttc
    13321 cttcctccat aatcgtaagg tcggctgcgg tatcggtatc aaacacttta tcaaatccca
    13381 ttttcttgat agctcccacc atttttccgg taaccggtgt tcctatcggg taaccgaact
    13441 cttcgcctat tccggctctt accgaaggag cggtttggat tacgacatat ttatccggat
    13501 cttttatagc atcccatacg gcttgtgtat tgtccttttc gtaaatagca cctacgggac
    13561 aaacatttat acattgtccg cagttaacgc agttgacatc ggcaatggat ctgtgatcgg
    13621 tagtggatac aacggtttcg tatcctcttt ccattacatt tattgcaccg actgtctgta
    13681 tattgttgca tatggatgta catcttctgc acagtacgca cttattgtta tctctgacta
    13741 tggattttga aatattatct atcggtaatc ccttcggtgc accgtcaaaa ggtatatctc
    13801 ttacattcag cttatcggcc agagcctgaa gctcgcagtt ctgatttctt atacatgtaa
    13861 gacaacttct gtcgtgatgg gaaagtatga gttccaatgt agcctttctt atatctctta
    13921 tcttgggaga attggttata actttcaaac cttccgagca aggataaacg caggctgcct
    13981 gcagtgctct tcctccgaca tccacaagac acattctgca tgcgcctatc tcattgatat
    14041 cctttaaata acaaagtgtc ggtatatcaa catttgccaa ttttgcggca tccaaaaccg
    14101 atgttccttc cggtacctgt acctttatgc catctattgt tacattaagc aaattcattg
    14161 ttaatcctcc ctatttcttg tatatggcac cgaatttgca tttttccata caaactccgc
    14221 atttaataca tttatcttta tctatcgtat acggtgattt aactacaccg gtaattgcat
    14281 ttgcagggca gtttctggca cagagaccgc aaccgataca tttctcagcg tcgatattat
    14341 aacttaacag gttcttacac actcccgcgg gacaggtctt ctgataaata tgagctttat
    14401 actcatcctt aaaatatcgc aatgtggaaa gaaccggatt gggtgctgtc tgacctaatc
    14461 cgcataatgc cgcttctttt atggaataag caagtctttc caatttttcg atatccttat
    14521 cttcaccttt gccggcggtt atcttctcca gcatttccaa cattctgtgt gttcctattc
    14581 tgcaaggagg acatttgccg cacgattcat ccacggtaaa tcccaagaag aaatgagcga
    14641 tatccaccat acagttatcc tcgtccatta cgataagtcc gccggaaccc atcatggagc
    14701 ctattgcgat aagattgtca taatcaatcg cgacgtccag atgctcggca ggaatacatc
    14761 cgcccgaagg tccgcccgtc tgtgccgctt tgaaagcttt accgttgggt attcctccgc
    14821 ctatctcgta aataacttct cttaatgtca ttcccatagg tatttctata aggccggtat
    14881 tattaatctt tcctccgact gcaaatactt ttgttccctt gctcttttcg gtacctatct
    14941 tattgaacca atcggccccc ttgtttataa tcaccgggat gttggcaaaa gtctcgacat
    15001 tatttaatat agtgggtttt ccgaacagcc ctttatttgc cgggaaagga ggtctgggtc
    15061 tgggttctcc tctttttcct tcaatggaag tcatcaaagc ggtttcttct ccgcagacaa
    15121 atgcgcccgc tcccagtctc agttcaatgt caaaggaaaa atcggtaccg aatatgttat
    15181 tacccaacag accgtattct ttggcctgtt ttatagcaag attcaatctc ttgactgcaa
    15241 ccggatattc cgctcttacg taaatgaatc cctgatcgga acctatggca tatcctgcga
    15301 ttgccattgc ttccaatatg ctgtgcggat cgccttccag tacagatctg tccatgaaag
    15361 caccgggatc gccttcgtcg gcattacagc agacatattt ttgttcattt tgagctgctg
    15421 cagcaaattt ccattttaat ccggtaggga atcctgctcc tcctctgcct cttaatccgg
    15481 aatccagcag caactgaata acttcggcag gtttcatctc tgtcaatact ttgcccaaag
    15541 caaaatatcc gtcgttcttt atgtattcat cgatgttttc cgggtttatg ttaccgcagt
    15601 ttcgaagagc aattcttatc tgctttttga aggatttggt ttcctgtata ttctttatac
    15661 atgcttcccc gtcatcgcaa ataaggtagt cggtacaggg ttttccttca ataatatgct
    15721 tctgtataat atcctgcgct ttttcagcat ccactttaac ataggtgtat ctctcacctt
    15781 ttgcagtata tacttccaca ataggttcta atcgacagat acctatacaa ccggttatct
    15841 gtatatcgac attcgaaagt ttcttttcat cagcaattct cttaagctct ttaaaaaccg
    15901 ggttggctcc ggcagcgata ccgcatgttg ccatacctac aacaatcttg taatccggca
    15961 cttcggtaac tttttgcttt attgctttta attcagttaa tgatttcatt aatatcctcc
    16021 atttacccga ttaatacttt ttaagtatat cggcaacatc gcttaccgcc aatgaaccgt
    16081 atacatcatc atttatcatc attaccggtg caattccgca tgctccgaca catctgcacg
    16141 catccaatga aaactttccg tctttcgtgg attgccccac atcaattcca agttgctttt
    16201 taagttcttc cagaatattg ttcgctccct taacataaca ggcagttccg agacatatct
    16261 gaatcttata ctttcctttc ggataaaggg aaaagtgggt atagaatgtt actacaccgt
    16321 atacttcaga tagcggaatc tccaacgcat cggcaacagc ttcctgaacc gataaaggaa
    16381 gatatccgta tatttcctga gcttcatgaa gaacaggaat caaagctcct tgtgtatctt
    16441 tgtacttgtc aaacacttcc tgaagctttt tttgattctc gtccagatta caattttgac
    16501 acatttatta gacctccgaa ttagttttta agtataacct tacgtattat atcacacagg
    16561 aatgttattt caataacaat attgaaaaga gtgttaattt aataacattt ttacacatat
    16621 tttattactc atatcacata ataaaagata aatactgaca taaaataatc taatcaatac
    16681 gagttccttt actgattgtg aacatgtatc ggaacttgga atctaatatg ttaatatccg
    16741 cataataacc gggtcttaaa atacctcttt tcttcaggtt cattactttt gccgggaccg
    16801 atgttgcgga tttcaatacc gtaaccggat taaaccccgc attaatcata ttgaaaacgc
    16861 cttttgccat ggtgacgctg ctgcccgcta ttgttcccga ttgatttttc accaggccgt
    16921 cttttacaaa aacatccgaa tcgcccaatg tatattcccc gtccggcata ccggttgccg
    16981 acatggaatc ggtaaccatt accagattct ccggcttctt acacttaaac aatatcttta
    17041 gcatttcttt accgacatga taaaaatcgg gaatatattc gcatgaaaca caatcgtcta
    17101 caagagcggc acctgtaacg gtaatttcac gatgatgcat taaaggcatg gcattgaaat
    17161 gatgagtaga cagtctgtaa cctgctttaa atgctttgta agcctcatcc gagatacatt
    17221 cggtatgtcc gaaagataca accttattgt gttcttttaa atatccggca agcttatagc
    17281 cctgatccaa ttcgcatgct attgtaaccc tctttacaat attttcatat tggcctgtta
    17341 tcttccgata agattcaaca ctcggttgca taatatattg cttgttatgt gctcccgcct
    17401 ttttaatgtt tataaacgga ccttccagat gaataccgca aaatgcggga tgttttattt
    17461 tactgtactt tgaaagcagc aggtttaatt tatcgggatc catggtaacg gttgtgggaa
    17521 agaccgatgt tgttccgact tttacgtaat tatcgaaaaa tttatcggca tcggcatcaa
    17581 acgaataaga aaaatccgta ccgcatattc cgtgggtatg aatatctata aatcccggaa
    17641 cggctataca accgttgatg tttatcgaat gaggttcatg agtatatgtc cttatgtcgg
    17701 tgataagagc gttttcaata tatatgttct gcagtccttc tttaaaatcc accccgtcaa
    17761 atacaacgca attacttaaa atcatggtgt acctccttaa agttttgctt tggataagta
    17821 atccagctct cttccgagca actgtctgta tattgcatta ttccgtatct cttcctgcac
    17881 ggctttctga gcatctttaa gtatatccat atcggtataa aggttggcta ttttgaaatc
    17941 cggaagacca tgctgttttg taccgaagaa atctccggga cctcttaata gaagatcctg
    18001 ttcggaaata tagaagccgt cattggattg taccattaca tccattcttt tctttgaaat
    18061 atcggatgat gattgattga acagtataca gtaagattgt ttatcgctgc gacctaccct
    18121 gccccttaac tgatgcaact gagaaagacc gaactgctga gcatcttcaa ttaccattaa
    18181 agttgcattg ggtatgtcaa ttcccacttc aataatgctt gtggtaacca gaacacttat
    18241 ctcattgttg atgaactttt ccattacttc atttttctcg acttttttca tcttaccgtg
    18301 aagtactgca ctttttacag atggaatatc cgtctgcagt tgctcgtatt ttgtaagcac
    18361 attggtcaca tccaagacat cattctcata gatagcagga catacaacaa atgcctgctc
    18421 tccttttaca acaatcttct gaatgaaatt attaatcctt tgtctgaatg attcatcaac
    18481 ggaataagtc agcacttttt ttctgttttt tggtaattcc ttcagtgtgg aaatatccag
    18541 atcaccgtac agtatcaatg ccagcgttct gggtatggga gttgccgaca tgaccagtaa
    18601 atgaggtttt acccctttgg aagacagtat aagtctttgc tgtactccga atctgtgctg
    18661 ttcgtcggtt atacacaaag cgagattatg gaatattaca tcttcctgta atatagcatg
    18721 tgttcctatc acaaggtgga cttttccgtt ttcaattcta tttaaaatat cttccctttc
    18781 ctttttcctt aactgacctg taagtaacgc tatatttaat ccgaatttgt tcaacagttc
    18841 aacagcggtc ctataatgct gcagggcaag tatttcggta ggagccataa aagcactctg
    18901 ataatcgttc cgatatgcac acattacggc atacaaaccg acaaccgtct tgccgcttcc
    18961 cacatcgcct tgcagtaatc tgtgcatgga atatccgctg cttaaatcgg aagctatatc
    19021 tttcatagat tgaagctgtg catcggtggg agtaaaagga agagatcgta caaattcccg
    19081 tgccatatca aacttggata tatttatagg ggatttatct tttcccatgt tgctttttaa
    19141 tgaaaccaat gccagttgca ttaagaagaa ctcttcaaaa accagtcgcc ttctggcagc
    19201 aaagaaatcg gcagatgatg cgggaaaatg aatatttttt accgcaaatt ccttatcgca
    19261 tagattatat ctttctttca aatattcggg aaagtaatct tttaccaaca catgagaata
    19321 atccatagcc gatttaacgg cttttcttat ctcatattga tttatcttag cattcttgga
    19381 atatatcggc tgtataatga aaaagtcttt ttcattgtag gaaaaatcgg gattgtacat
    19441 attgtagaaa aaaccctttt tctctacttt cccgtaaata taaatcattt tgttctcttt
    19501 tagaacattt ttcagataag gttggttaaa ccatgtaacg gaaatctgtc ccgtctgatc
    19561 tttaaactcc gcggtgacaa tatgtaatct tctgccgtaa ggtaattgtt tggaagtgat
    19621 gttgtataaa atacccttta aagagaaata ttccccttcc cttatatcgc ttatatgtga
    19681 aataccggtc ttgtcgacat aggttctcgg ataatactcc agcaaatcat ttgtgttgta
    19741 tatgctcaga gtattcagct tttcaattat tttcggtccg atacccttaa gggctgccaa
    19801 ttcattcatt attccgttcc agtttttcga ttattttgtt taatacaacg gaacaggaat
    19861 cataaaaaac ataatccgct tttgaatcac ggggagtgct tgtcttgttt aatatacata
    19921 ggcaattctt ggtatagaag tcaataaagg aagctgcggg atataccgat aaacttgtcc
    19981 ctgcaacaat caatacatcc gcattcataa tggcttgtat ggattgctct attataacct
    20041 gatccagctg ttcttcgtac aataccacat cgggttttac caatgaatta cactcatcgc
    20101 atctgactgt tttacctctg ttggctatta tatagtccag attgaaaaac ttatggcact
    20161 tgatacaatt attcctgctg atacttccgt gcaattcgta aaccttaccg ttaccggcga
    20221 tattatgcaa atcgtcaata ttctgtgtaa caacggacac atcatagttc atgtctttaa
    20281 actttgttat accgtaatga aaatcattgg gctttgcttc aggatgcaca atcttgtcat
    20341 gatagaagcg ataaaacaca tcgggattat gatagaaaaa actgtgactt ataataactt
    20401 cgggagtata gccgtcatat ttgcttaagt aaagtccttt ggtgcttcga aaatccggta
    20461 taccgctttc ggtagacaca cctgcaccgg tgaaaaaaac tatactcttt gcctttttaa
    20521 tttccttaac caaattatca atcatcacaa cacatcaata aaatactcca acatgtctgt
    20581 cttcttggca gttaacttgc gtatacctat cttgttctcc aactttttat caataaaatt
    20641 gcaatcaggt aaagcggacc acggttctat acacacataa tttgtgtaaa aagggtgggt
    20701 ccaaattccc aaataatcaa aatcttcaaa tctcactttt acttttctgc tcgaagtaga
    20761 acatttcaga acaacttccc tgttgcttaa ttttgaacaa tccagaataa gggcatcatt
    20821 cttaaaaatc tcaggcttaa gatacagttt cctatcggaa agttttatct cttccgtttg
    20881 cttattaatc ataatattct tatcggttgt gtaattatat ataatattgt ctgagaacaa
    20941 aatgtagtag tcgtccatta ttttattctt ttcaaatgtc agattatacc cttcatgtcc
    21001 cccgacttca taatacatca ccttataccc gttatttatt atcatgtatt gcttcttgat
    21061 tatgttatcc atcagttcgt aggaaactat aaagtcgaaa tcaaacggat atacggcata
    21121 agtatcggga gtgccggtaa tcctcaacga tacataattc tcattgctct ccacaatctc
    21181 aaaaagcgaa tgtctggcaa atccgtgtat ctttacttta aacagtctgt tatcatactc
    21241 atattcctca tctttcagtc ttcctataaa aggaaacaaa ttgggagcct gacctttcca
    21301 gatatcggga tttccctgcc atagatattc atatccgtct ttaataacgg aaactatttc
    21361 cgctcctaac ggattgataa taacggttaa atgtctgttt ttaagtttaa tcatatctgc
    21421 ctcacagata atattatatc acaaccgtta ttgatgtttc agtaactata tgatatattg
    21481 aagcagttag gaggatgaca tgaaaaacaa aattgatata aaatttcttt caataagcga
    21541 tgtcaaggaa tttgcggata tagtctccgc ttttgattac gatatcgatc tggtttccgg
    21601 cagatatgtt gtggatgcca aatccattat gggtatattc agtttagact taagtaaacc
    21661 catatcactg gttgcacata ccgatgacat taagccgtta acagacgcta taaagagatt
    21721 ttgttaaaaa caaacgggtt accccgtttt tttttgcaga aaatttgatt ttactgatat
    21781 gtaactgata tcccactgat tattaatatc aatttccggt attttttaat aaatctgtat
    21841 tgataataaa acttacttac tcttttcaat aagtttctta accgtattgg cgatatatgt
    21901 atcttccgta agctctatca ttcctctgtc cattgcagca cttaatcttt tatcaaaagg
    21961 tatcctgtcc acaaccggaa tactcagttt atttgtatcc gtatcaccga aaagctcaat
    22021 tttgttatta catttatcgc ataccgcata actgaaattc tgtatggctc ccaaaaccga
    22081 aatatgcatc atatctgcca ttttaattgc tttttccact atcatggata caagatcctg
    22141 cggtgtagta actattatgg cttggtctac aggaatggac tggaataccg taagagcggt
    22201 atctgccgtt ccggggggca tatctatgag cagataatcc aattctcccc atacaacatc
    22261 ggtataaaat tgtttaagta caccggaaat aaccggaccc ctccacaaaa caggatctgt
    22321 cgtattttcc aaaagcaggt ttatgcttat tatttttgta ccgaaattgg tctttgcggg
    22381 aaatattccc gtctcatttc cccttatatg ttcgcttaag ttgaaagctt taggtattga
    22441 aggaccggta atgtccgcat ccagaatgcc caccttatac cccgctctgg taagttccac
    22501 agccagaagg cttgttacca tgctttttcc cactcctcct ttaccgctta aaacggcgac
    22561 aacattttta atctgagaaa aatcattggt ttgttctttt tgtattctgc tctcgcaatc
    22621 attctgactg cagtcagagc agttgtgatt acattgtgtc atatttgctc cttctatctc
    22681 tcgtaatata ttccgttatc cggaacctta ccgcctaccg aagcggggtt gttgttataa
    22741 agaaatttaa aaaatgcgtt taaactgtct tttgcatccg ctgcatcgat atagacaatg
    22801 ttgcaattgg gtattgcact ttccgctacc ttttcgttat ccagaatacc cgatgatact
    22861 atgagaggag cggcattgta cggctgctct aaaacccaat ctatagactc ttcatataac
    22921 tcaaggaaag tatccacttc ctctttatat tcctctataa aggacttctg tgccaccaga
    22981 cagcccattg acaacgttat atccggtgca cttttaaccc attcatcatt cagatttatc
    23041 gcatgattta tggaagcatc tttattcaaa acggtggaaa caaacggttc cggaagcatc
    23101 acaacgtccg cttcccccga aagaagtaat gtggcgactt cgctgtgttc cgctttatac
    23161 actatttcca tatccgtttc cggattaatg ccgttttcgg ataatataaa gttcaggaca
    23221 tattgcggca acgaaccttg tccgcttgta acaagggttt tacctttcag atcttttata
    23281 ctcttgatat tgcccgtctt atccaagata tgcagtacgc ccaaagtgtg aaccgccaat
    23341 atctgtattt ctccttccgt cttgttgtat agaacagctg caaggtttgt aggaacggca
    23401 gcaatggtta tgcttttatc cagtaatccc gcaacaacgg tgtcgggtga agtcattatt
    23461 ttaaattttg tgttgacata atcggaaaga gagattatat cattcattac tttgaccatt
    23521 cctataccgg taggtccttt caatgcaact atatttgccg ttaccggttt taccggtctg
    23581 tgtgtctcgt atacttttct gcatccggtt aaagacaaaa ccgttaatac tacaagtatg
    23641 atagataaaa ttcttctgtt tttcataatt tttctccttc tattctaaca gtccttatta
    23701 ggattgtatc tcaattataa ctctaaaaat cattattaca acaaggaaaa cttaaattgt
    23761 tccaaatcaa taatatttat ccccaaagcc tgagcttttg tcagtttgct tcccggctta
    23821 tctcctacca gaacgtaatc ggtctttttg gaaacgctct cggtaacatt gcctccgttg
    23881 gaagtaataa tacttttcaa ctgatctctg gtatattctt caaaggagcc tgttataacg
    23941 tatgtctttc cgataagttt atcactcagc ttctgtgtat tgttataact cagagacttc
    24001 atattaactc cctgagcttc cagtctcttt ataagttcaa cagctttatc gtctttaaag
    24061 taatcgacta tactgtttgc cattacctct ccgaatgtat ctatggatgt aaaatccgtt
    24121 atttcggctg ccataatact cttcatgtcg gggaaatttt ccgcaagcaa tgaggatgct
    24181 ttctgtccta tatacagaat tccgataccg aagagcagtc tgtctatatt attgctcttg
    24241 gatttctcta tagcagaaag cagtttattt acacttttct caccgaatcc ttcaattttc
    24301 agcagttgtt ctcttttatt atgcaaatca tatatgtccg caatatcctt taacaatgat
    24361 aaatccatga acttttccac agttgcagta cttaatccct cgatatccat tgcatctttg
    24421 gaacaaaagt gttccagacg cctggaaatc tgacctgtac agtttatgtt ggtacatctg
    24481 tatgccgcct gatctttcac tctttgaaca tcggaattac agaacggaca attctctggc
    24541 attttaaact taatttcctt ccctgtcctt ttatctttta aaactctgac aacctccgga
    24601 atgatatctc cggctttctg aattattaca tggtcattaa ttcttatgtc attggtatcg
    24661 ataaaatcca tgttatgcaa tgttgctctg cttacagtac tgcccgacac aaaaaccgga
    24721 tcgaattcgg cattgggagt cagtacccct gtttttccta tctgtacaaa tatgtcttta
    24781 accacggttt ccttctgttc gggaggatat ttatatgcaa tagcccatct gggagcctta
    24841 gcggtggagc ctaaaacttc cctgtccgca atattattca gcttgattac cgcaccgtct
    24901 atatcgtatt tgtaggaaaa tcgtttatca tgtatttcct ctataccgga aataatgtcc
    24961 ttcatatcac ccgatactct tataacatca tttacgacaa aacctgcatc tttcagaaaa
    25021 ttcaatgtct gaacatgggt atcgaaaagt gctccctgta tactttgcag attgaataca
    25081 aatatcttca gatttctttt ggccgttatt tttgaatcca actgccttag tgagcccgca
    25141 gcggcgtttc tcggatttgc gaacggtttc tcaccgttct cttccgcaac ttcatttatc
    25201 ttagcaaaat tatccttatc catatatact tcaccgcgaa ctacaagata aggtattact
    25261 tcttttgtat gtaactttaa aggtatgtta tgaacggttt tcagattttc ggttatatct
    25321 tccccgtata ttccgtctcc tctggtagca cctcttacaa atatattatc cgcatactcc
    25381 aaagtaaccg aaagaccgtc tatctttttc tccaacacat actcagggtt aatactttct
    25441 tttaatatcc tttcatggaa agcatacaat tcctcgtaag aaaatgcgtt ggacaaactc
    25501 tccaacggaa ccgtatgttt cacttttgca aattccttaa gaggttttcc cccgactctt
    25561 tgtgtcggcg attcaaccga tctgtattcg ggatattgct gttcaagagc gataagctca
    25621 ttcatcagca tatcatactc atagtcggat atctcgggtg catccaattc ataatatctt
    25681 ttgttgtgat aattcaatat ttttaccagt tcatcaattc tgtttttcat gtatatattt
    25741 tatcatattt aagtggataa taattgatat tttcagtata atgtgttata aatattcagt
    25801 aattcatttg gaggtggata ctttggcaaa taaaaaatca gcggaaaaaa gagttagtgt
    25861 ctcaatgaag aaaagattgg ttaattcggc aaaaaaatct tctaccagaa agcagttaag
    25921 agatgccaga ctggcaatag aaaatgactc gccggataaa aagcaactta tcatggaagc
    25981 aatgaaagct gttgataaag cggcttccga cggcataata cacagaaata aagctgccag
    26041 acataaatct aaattatcgg ttcatcttaa ataatcgttt aagaaataaa tctatattta
    26101 ttaatataaa tccgaacgga ctccgttcgg attgtgtttt atatatttct gcataaatga
    26161 tgtattaccg gttacacctt atatagcctg tctcatccaa cctttgtttt tcttatccag
    26221 cggttctctt cctaaaatct ctcttgcagt tttaacatag tatctgtaca actccagtga
    26281 ggaaccgttg ggtattctgt gatccagacc gaaaacaata ccgcctttta acattgaagg
    26341 ttgcatctta tactccaact cttttaatac cgcaagttta ctgtctctta aaatgaactt
    26401 atctataccg cctttaagtg ataaccttgt accatatttt gcgtgtactt ttactatatc
    26461 cattccagca gcaggttcca ttggatacat ctgattcaat ccgcaattca gaaaagcatc
    26521 tatgacggaa ttcatgtttc cgtcggagtc ctgaacaaat attgtacacc cgttattttt
    26581 gtaagcatcc catattttaa gataataagg ctgtaagaat tcttctatat gaacaggccc
    26641 tataagcgaa ccggattttc ccgccatatc ctcatgaatg tgtaaatgat cgacaattaa
    26701 gtgcttggaa agtctctcgc aacatttaat agcggtatcc gccgcggtat tgagaatatc
    26761 ttctatcagt tccggttgtt catagtaggc ataacagagt tcctcttctc ccatcaattg
    26821 tctgggtaaa tcgaatccgc ccggaacttc cgcccgcacc ataacacctt gtttttgctg
    26881 ttctttagcg ttttctacct ggttataatc tatcctttct tccctgaact gatacagatg
    26941 cttcaatttt aaccagtcat ccatattttt aacgggataa tccattggta gcggtatggt
    27001 tgcggctcct ttcatcaatt tcatttttct tcccaaggca tcgatagtga taatatgttc
    27061 ctttgtttct tctattactc tttcttcagg accgtcaaat atatcgcaat ttgcaccgca
    27121 gttaacatac ggaacatagt cccagtcaaa tgccgtcata tttatttcat cttcggtagc
    27181 gccctgttca atccattcct cttccagacc tataagcggt ccgaacagtt ctacaaacat
    27241 aggcctttta ttctctttaa acgttacata atccaaatat tcttttctgt cccagcgcat
    27301 atttttacct caacctccgg tattataaca taatattaag aatttactat tgttgacatc
    27361 aatgttacca ttgccgaatc ctgaccgagt tttcccgact tgaaatcatg atcggcttcc
    27421 gatatggcat atataatatt ctttaattgt tctatactga actctttgga attctgaatg
    27481 gcttttctca ctcgaaaatc gtttgaggat gtaatattat taactataat cgtatacatt
    27541 tcatagaaag tattgaaaag aatagcggta aaatacggta aagcgtattt taaagatacc
    27601 atatcgtaat agacatccag tgccgcagat aaatctctct tacaaagatt attggtaagt
    27661 tcatatcctt ttacatcggt aataccggtg cataacaatt ttatcgtttc cgcggttact
    27721 tcattggagg gctgaatata tagcagaatc ttatctatac ttaatgtcac aaaggaaaga
    27781 ttaagaccgg aatattccag aaaaagattg agattttccg aagatatgtt cttcccctct
    27841 tttctgaaag cggaatatac aaatctctta atgtcctcag cggttccttt ggttatctcg
    27901 taagcactgt ttttggaatt atagtgctta aagcctgaca gtcttttatt gatgtcgttt
    27961 tcaatgaaga ataagtagac accttcggta acataatcgc taagactgat aattttatca
    28021 ctaaattcct ctttgttgaa aaatccggta ttcctgcagg ttataaattt attgttaccg
    28081 ctgaaggaat aagtggcaca ttcgttaatg atatcctcta tttttgaagt atcatcaaac
    28141 tttacattgt catattccgc ttgtgtgtat cgtataattg cggtttttaa agcatttact
    28201 accgattgct taagatactc gtcgtccccg taaaacagat acacctgttt aaaaatgttg
    28261 cccgatattt catttttaat atcttcgtat ttcacacata gattttaaca catttcatct
    28321 gtatttaaac agatagtatt ttctgtttat aagatatacg gtaaccgatc cgttaatatc
    28381 cgttcgcaag gtatttatac ctttttccgc aagtgtatcc agagtttttc ttcccggatg
    28441 accgtacctg ttatgttttc cgaccgatat aaccgctatt ttcggagata tataatgaat
    28501 tatttccgaa tttatagcct tatcatctcc gtgatggggc atcttaatta tgtcacattt
    28561 taaaaatata tctgaaggta tcttttttat gtccgcatct ccggtaaaaa gaaaactacc
    28621 ggcttttgta tacagattaa aaactaccga accgtcattt gcatttgtat aaacgatatc
    28681 agtatcgggg tttagtactt tgatacagac tccttcggtt cttatctcat cacctctttt
    28741 gagcataccg caatatgtat ccggataatt catgctcaat tggtcagata gtttaatatt
    28801 gtccttatga tttgtatatg gaagataaac attatccacc gagaagttat ccaaaacatc
    28861 ttcaattgca ccg
//
LOCUS       DAOSOM010000026        28508 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_1659_length_28508_cov_8.848065, whole genome shotgun sequence.
ACCESSION   DAOSOM010000026 DAOSOM010000000
VERSION     DAOSOM010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 28508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28508
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1659_length_28508_cov_8.848065"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..1414
                     /locus_tag="PLF14_06905"
     CDS             161..1414
                     /locus_tag="PLF14_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006864063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOT71054.1"
                     /translation="MANYTTKTYSMKREIVNFSKDLTKNLPLPVKKFITDMNYGMLAS
                     NSCLLSDIADTLHEETKKKNTIERLALNLEKGIPDKLNRNYLKKVKNIVGKEPVVHID
                     DTDIVKPCGRKFEDMGRVRDGSESSSEKNVYKNGYYVTEACALTTNNHPVSVFSKIHS
                     SREEDYISTNAITFQAIDESVKLFKKATFVMDRGYDADKIFFKLEKLRQEYIVRLTVK
                     RKVVRNNRKISVSELCNRRKGKIKITVRYNDKDYDAYLSHVKVQLKEDSNYVYLVLVY
                     GVTEHPMMLLTNKEIKSKEDVIRIARLYFSRWRIEEYFRSKKQIFGFEKFRVRSMVSI
                     RSLNFMITACMTFLALMSMKSVRSSLLIEILRRAAPLKQKVQFYYYRIAKGILKILSY
                     ARKGVRGWFKVKRQKYRQLAFSFLS"
     gene            1717..2937
                     /locus_tag="PLF14_06910"
     CDS             1717..2937
                     /locus_tag="PLF14_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT71055.1"
                     /translation="MYIKRHIEFAVSERAREKGAVIVTGARQVGKTTLIENIKPDISR
                     VTFDDITVRHRVKKDPAAFLQLNPPPLFIDEVQYVPEIFHYIKISLDRSRNKGDFYLT
                     GSQSFELMKNVSESLAGRAGILNLMGLSLREMRNENWNEPFIPTTDYLMRRKAAKIDL
                     NINEVWKIIHRGCMPELFKEQSYNWQNFYSDYIKTYIERDVRNLTQVADESDFMKFMT
                     VCAAMTGSLLNIASLAKEVGISQPTAKRWLSILKTSNIVYLLKPYSNNAIKRAIRTPK
                     LYFLDTGLAAYLTRWLTPETLSTGAMNGHFFETFVVSELIKSYANRGMDEYFYFLRDS
                     NGREIDLLIFQDNTLFPIEIKKHTNPSYRDIASFDMLKSVKNITIGEGGVICPSQDLL
                     PLKDNHKIIPLWAV"
     gene            3030..3593
                     /locus_tag="PLF14_06915"
     CDS             3030..3593
                     /locus_tag="PLF14_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOT71056.1"
                     /translation="MKILLELYWVFFKVGMFAFGGGYTMLPLLQKEIVEKKKWLTSEE
                     IIDYYTLSQCSPGIIAVNVSTYIGFKKKRYLGGIVSALGVATPSIIIITVIAAVITNF
                     LHIKEVSYAFAGIRVAVAALVIVTIVDMYKKSVVDWLCIILYITSFLLSVLTSISPVF
                     IVLGAGITGLVLGTRMRERKGDKNDIS"
     gene            3580..4146
                     /locus_tag="PLF14_06920"
     CDS             3580..4146
                     /locus_tag="PLF14_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOT71057.1"
                     /translation="MIYLELFWEFFKTGLFSVGGGLATIPFLYDIGRRYDWLDTSIIP
                     DMIAISESTPGPIGINMATYTGYTAAGILGALIASIGVILPSVIIVLLISRFLVSFSQ
                     DKRVLNTMYGLRPASLGLISAAAFTLLNVSVITYDAFVESGNILDIINIKALVLFVCL
                     LVIRLFFRKIHPILFIVFAGVIGVIFKF"
     gene            4156..4884
                     /locus_tag="PLF14_06925"
     CDS             4156..4884
                     /locus_tag="PLF14_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015043876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HOT71058.1"
                     /translation="MTDILRILFSAIAPVAVLIILHLVIDRHDREPAKLLILQFVAGC
                     LTSIPAIFIENFLEGLNIFSGTILGVAYTSYIVAGLTEESLKRASVYLVSYHKPAYNE
                     KLDGIIYCCFASLGFAAVENILYIVNFQAENPSIALYRGIFSVPGHMLFAVTMGYYIS
                     LSKFAPTYALMRSYRVKSLLYPVLLHGTFNFILSLNIHWLIAFVPFVIFLWIFNIKKL
                     RSFSKESRYYKNLYDNNNPGNTSI"
     gene            complement(4949..5284)
                     /locus_tag="PLF14_06930"
     CDS             complement(4949..5284)
                     /locus_tag="PLF14_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71059.1"
                     /translation="MARKKELYVLAVKDLDKTLADIAAGKYKMPVENSKYAEIFATIV
                     RRCDNLDELPKFIRKAKMKKSECIHWWEGIIEDGYELFIVQYNAPDENFVELAGSEEV
                     VKFVVSVKK"
     gene            complement(5397..7646)
                     /locus_tag="PLF14_06935"
     CDS             complement(5397..7646)
                     /locus_tag="PLF14_06935"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HOT71060.1"
                     /translation="MLTENAKKVLEKRYLKKDSEGNVIESYDDMFKRVAKTIASADSD
                     YLGKEDIEKTEKDFYDMMINLEFLPNSPTLMNAGRPLGQLSACFVLPIEDSMDAIFET
                     VKNAALIHKSGGGTGFSFSRIRQKGAAVSTTGGIASGPISFMKVFNIATEAVKQGGTR
                     RGANMGILRVDHPDIREFITCKANSTELNNFNISVGLTEQFMQAVEHDNMYELIDPST
                     KKTVGMERAKDIFDMIVEHAWLNGEPGIVFLDRINKKNTVPELGEIESTNPCGEQPLL
                     PYESCNLGSINLLTMIKEDKSGFDYEKLERCVIKAVHFLDNVIDVNIYPNEKIAKNTK
                     ATRKIGLGVMGWADSLYTMGIPYNSEQALELGYEVMKFINEQSKKASMSLAEIKGVFP
                     AYDKSVYPAQDIKVRNATTTTIAPTGTLSIIAGVSSGIEPVFAISYIRNIMDNTEMVE
                     INPVFEEVARKRGFYSEELMRDIAKKGSVSRMTDVPEDVKEVFVTSHDIDPSWHIRMQ
                     AIFQEHVDNAVSKTVNLRNEATLDDVREVFMQAYALGCKGVTLYRDGSRAAQVLNIPK
                     QQHKEAVQPGTVLIPKQRPRITTGLTEKFRTGCGNLYITVNYDENGEICEIFTNTGRA
                     GGCPSQSEAVARLVSIALRSGIDTAAIIDQLKGIRCPSTIRQKDLGILSCPDAIGRMI
                     EKVAKMNGGLKEEVKRTVPYLHYGETKNSADKNEKSFESRLCPECKAPLESDGGCMIC
                     RNCGYSKCN"
     gene            7839..8456
                     /locus_tag="PLF14_06940"
     CDS             7839..8456
                     /locus_tag="PLF14_06940"
                     /inference="COORDINATES: protein motif:HMM:NF021617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HOT71061.1"
                     /translation="MEVRHKSAIPIYIAGAVWVLYSIIFPMHKLIHLVIATVLFIVTF
                     FLSRRFFPDRIEMVEEPIKYTGAKDADLAIAEGNRYIEEMRKANIRIQNAVVSEKIDI
                     LSDVIKKIFAYITEKPEKVKDIRRLITYFLPTTVKLLNAYARAEEQNNHGENITQVKT
                     DIEGAMDGIIHSFRKYLDNLYADEAMDIDAEITVFDSMLQAENLK"
     gene            8474..9508
                     /locus_tag="PLF14_06945"
     CDS             8474..9508
                     /locus_tag="PLF14_06945"
                     /inference="COORDINATES: protein motif:HMM:NF017617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HOT71062.1"
                     /translation="MNEITLTEKDRELALSYGEKLDIKSSASILQYGADAQRKITTFS
                     DATLEKIRSKELGEIGDMIGNLMAQLKDFTADRESGNFLERLFRKTKRYIDVLKARYT
                     KVEANVNKIVAVMEDHQVVLMKDITMLDKMYEMNLDYIKELALYIEGGRAKHKELKET
                     KLVELRKKAKETNLPEDAQAANDFANMLDRVEKKIYDLEITRNIAVQMAPQIRLIQNN
                     NTLLTEKIQTTLINTIPLWKNRMVLTLSITHSQNAMKAQRAVTDMTNELLLKNAEMLK
                     QSSVDIAKESERGIVDIETLKATNESLITTLEEILRIQEEGKAKRKEAEKELDRIEEQ
                     LKSKLLEFKN"
     gene            9508..10035
                     /locus_tag="PLF14_06950"
     CDS             9508..10035
                     /locus_tag="PLF14_06950"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOT71063.1"
                     /translation="MKFKENRALARILVVIAVIASVLISGHVSLSSQRRAVMNSFYDT
                     MDADLNTKSAYADNLSGVASRYLDRNSEYITNMAQARDLLLEAKTPAEKYAASVKITN
                     AAAALYDVLGTMSLNETDERLRRSNYADIVAVDDILKRTSFNQDVDKFNSQLALFPAN
                     VIASITGIDKAEYFR"
     gene            10066..11046
                     /locus_tag="PLF14_06955"
     CDS             10066..11046
                     /locus_tag="PLF14_06955"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOT71064.1"
                     /translation="MKKLAIFITLILILTCNAVFAADIPAPTSDFYVNDTANVLSEKT
                     KKTIIDKNLALYEKTGAQIVVVTVEYTHTASIADYAYELFNEYKIGSAEKNNGLLLLL
                     SIGDDDYYVLQGEGLERTLSSGTISYLLYEHLEPYFAKQQYDEGVLNIFNVLYKEMLA
                     IHDTTIEYEDVYPPQDNYYYPDDYFEQEDSSFSVFGFFATIFAIMRVIFFFMVIFGVL
                     SAIFGRRKSTRRSDVARNILPIISTLGYMNTLKNMNNTSNYSSFNNNSSTVNRSSYSS
                     GRSSYSGGGSSFGSSSSSSRSSFSSGGRSGGFSSGGGGSSRGGGAGRGGR"
     gene            complement(11107..11274)
                     /locus_tag="PLF14_06960"
     CDS             complement(11107..11274)
                     /locus_tag="PLF14_06960"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HOT71065.1"
                     /translation="MNIHFDIDNFLKTPRVVLFGMLGVFIALTIIYLAILLLSKIFKN
                     KKKKDSGATEE"
     gene            complement(11287..12417)
                     /locus_tag="PLF14_06965"
     CDS             complement(11287..12417)
                     /locus_tag="PLF14_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009221645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOT71066.1"
                     /translation="MADIFSNVLEIRWEMVLMWVIGGVLIWLAIAKDMEPTLLLPMGF
                     GAILANLPTSGAITQVINGKTEEGVLDVLFNAGIANELFPLLLFIGIGAMIDFGPLLS
                     NPKMLLFGAAAQFGIFFTFALAALLGFDLKDAASIGIIGAADGPTSIFVANYFNSNYI
                     GAIIVAAYSYMALVPIIQPPVIRLFTTKKERLIRMPYEQKEVSATVRILFPIVTTMIV
                     GILAPRSVALVGFLMFGNLLRECKVLRILSESAQKELANLVTILLGITIASKMRYDLF
                     VKVDTLVIILLGLVAFVFDTIGGVLFAKLLNLFVKKKINPMVGAAGISAFPMSSRVVH
                     KMGLKEDPTNFLLMHAAGANVAGQIASVVAGGVMIALVEKLI"
     gene            12564..13229
                     /gene="deoC"
                     /locus_tag="PLF14_06970"
     CDS             12564..13229
                     /gene="deoC"
                     /locus_tag="PLF14_06970"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOT71067.1"
                     /translation="MNIENILSKVDHTLLKQTAKWEDIKTICEDAIKYNCASACIPPY
                     FVKQAKDCFKNNLKICTVIGFPNGYSTTATKCFETARAVSDGADEIDMVLNINMLKDK
                     LYDEILTEINQVKKACSGKLLKVIIETCLLTEEEKIKMCEIISKSDADYIKTSTGFST
                     AGATREDIMLFKEHIKNGKLIKAAGGIRSLNDANDFISLGSSRLGTSSIVRIVKDEEN
                     TSY"
     gene            13347..14423
                     /locus_tag="PLF14_06975"
     CDS             13347..14423
                     /locus_tag="PLF14_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013244737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOT71068.1"
                     /translation="MKKLFSIVLVVVLLLSMLSLAGCQAKTYELAMITDIGDIDDKSF
                     NQGTWEGLRDYAVEHKITHKYYKPTEASTDAYIAAIDLAVSSGAKVVVTPGFLFEEAI
                     YIAQDKYPEIMFILLDGVPHDANYENFKTGPNVFPVVYAEEHCGYLAGYAAVVDGYRN
                     LGYQGGMAVPAVCRYGFGYVQGANDAARDLGLAKGSIKIMYNYSGDFADTPASQTRAA
                     GWYQAGTEVIFAAGGKVGNSVMSAAEAANKAVIGVDVDQSAESPTVISSAMKGLATMV
                     QYGLKGFYAGKFPGGEAVTVGAESNAVSLAPFSTSKWKTFSQADYDALFAKLANGTIV
                     PEKDLDRTNPTELKLDYVKLTFVE"
     gene            14594..16123
                     /locus_tag="PLF14_06980"
     CDS             14594..16123
                     /locus_tag="PLF14_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT71069.1"
                     /translation="MDYIIEMLNIRKEFPGIVANDDITLRIKKTEIHALLGENGAGKS
                     TLMSILFGLYKPEAGVIKLRGKEINISDPKTASHLGIGMVHQHFKLVEDFTVLENIIL
                     GAETVKNGFLKMDEAKKKVMELSNRYKLYIDPDAKVSNITVGMQQRVEILKMLYRENE
                     ILIFDEPTAVLTPQEIDELMDIMKELSKEGKSLIFITHKLEEIKKVADRVTVLRRGKC
                     IGTYNVEDVTKESLSEMMVGRKVQFTVDKKDITPGETIFEVKNLSMKTKGSTKNTLNK
                     ISFKVRKNEIVCIAGIDGNGQSELVYALTGIRHFDEGQILVHGEDWTKKSIRYRNSHG
                     MSHIPEDRHKYGLILEYSMAYNLILQTYFTDRFQKRGFLKNDSIHEYADGLIKKYDIR
                     SGQGAQTVTRSMSGGNQQKVILAREIDKDPSIMIAVQPARGLDVGATEFIHKKLVEQR
                     ESGKAVLLISLELEEIMNLSDRILVIYEGELVGDLDPKKTTIQELGLYMAGAKRDEVK
                     A"
     gene            16138..17289
                     /locus_tag="PLF14_06985"
     CDS             16138..17289
                     /locus_tag="PLF14_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008727541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT71070.1"
                     /translation="MNQFFDRLSRKKGTGSFISSLVAIGIGLIIGLIILLISNPREAF
                     AGFFTMLIGGFNDGTRSFGNVFYIATPIIMTGLAVGFAFKTGLFNIGASGQFIVGAYV
                     AVLIGVKLPQLGGFQWVLGILAAMAIGTIWGAVPGLLKAYLNVHEVIASIMMNYIGMF
                     MVNYLIKQTVLNVYTNRSLAVAPTANIPKMGLTAIFPGSSINGGFLIAILFVILMYII
                     LEKTTFGYELKACGHNRFAAKYAGINENRSIILSMAISGMLAGVGGALHYLSGAGVYI
                     EVVDVLAAQGFTGISVSLLGMSNPIGVFFAGIFIAWLQIGGFYMQRYSFVPEFIQIIT
                     AIIIYCSAASLLIRNYLELRQLKKDENLTADSDGDTADIVNVENQGGDK"
     gene            17289..18248
                     /locus_tag="PLF14_06990"
     CDS             17289..18248
                     /locus_tag="PLF14_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT71071.1"
                     /translation="MDMLYLLLRQTMYFSIPLLIVALGGMFSERSGVTNIALEGIMVM
                     GGFTGIYFINQMQSQMSGQWLLVLAIFIAMAGGLLLSLPHAYASVNLKADQVISGTAL
                     NMFAPAFVIYIARMIQPSGIANVSFKNTFFIHEVPVLCDIPVIGDILFKNTYITTFLG
                     FFILIVSAIVLYKTRFGMRLRACGEHPHAADSVGINVYKMRYIGVMISGVLAGLGGLV
                     LTVPISTEFKGAVNGYGFLALAVLIFGQWKPMRILWASLFFGFMQTISAAYSAIPFLL
                     NLNIPSEFFKMTPYIATLIVLAFSSKSSKAPKAVGQPYDQGAR"
     gene            18252..19553
                     /locus_tag="PLF14_06995"
     CDS             18252..19553
                     /locus_tag="PLF14_06995"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HOT71072.1"
                     /translation="MRMYDIIQRKRDKHQLTDEEIRYFVKGYTDGSIPDYQAAALLMA
                     IFLNGMTDHETSVLTLAMAQSGDMIDLSAVNGITVDKHSTGGVGDKTSLIVAPCVASL
                     GFKVAKMSGRGLGHTGGTVDKLESIPGYRTSLDRQEFIDNVNRIGIALVGQSGNLAPA
                     DKKIYALRDVTATIDCLPLIVSSIMSKKIAAGSQNILLDVKVGTGSFTPKLEDAVILA
                     QKMVDTGKLAGRNIAALITNMDVPLGNAIGNSMEIIEACEILKNKGPSDLREVCVELS
                     AYMVSLCTQDSYENCRRKVLQSLMDGSAFEKFRQLVSAQGGDVAFIDNTDKFKKAFYT
                     HDVLSSHSGYISSMDTANIGRVSLMLGAGREKKEDSIDFDAGIYIHAKTGDSIKKGQL
                     LATLYTNRKHVLPDAENEYLSSLTFSENLPPPQKLIYQIVK"
     gene            19587..20564
                     /locus_tag="PLF14_07000"
     CDS             19587..20564
                     /locus_tag="PLF14_07000"
                     /inference="COORDINATES: protein motif:HMM:NF027787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5050 domain-containing protein"
                     /protein_id="HOT71073.1"
                     /translation="MEKGKFIIDFKNRQHRVAVISVAIAVLLIVLAVLLFKVFNKSYN
                     NYEGNSPSNIINNGLIAYEDGHLYYTRFDAGSKIFRLDIESGKEKLVYAANAKYLNVL
                     EGYIYYIDGSNGNIKRITVSGTDELLLKDSICSNLYVTKDWIYYIDNKENNSIYRMSM
                     HDYIPEKLAEESATSVSVHDGFLYFINYMDNNGIYRLDLETLEVSPVLKQHTIFYTVK
                     DNLIYYTNTNDESYIYSMNLDGSGNKRLEGVKGWFLASSPDDPDFLYFMSPTGILKKV
                     NLASLNQMTVSNDECAYINIVEDYIVYLNRDDNHHYLLNKTSGKSTILR"
     gene            20612..23059
                     /locus_tag="PLF14_07005"
     CDS             20612..23059
                     /locus_tag="PLF14_07005"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOT71074.1"
                     /translation="MKKSVRITFALLLIIALCFSFVSVSAQEDFFDSRYYWLYSSAIP
                     SAWKLLADIPTEPVTVAVIDTGIESDNPHLKDRIDPRGRNFVPSPPSDNVEDKQGHGT
                     AIAGIIAAVPTEENNYYGVCGNLDVKILPLKVYGDITGTLQMRYIIDAIYYAIEMNAD
                     VINISIDIPNFDEGLQIACLAAYEKSIPIIASAGNKSSEYYSYPSSCYGVISVSALSL
                     YDDDYFLSSPFSSYNDQVDLCAHGEDIYTIGLNGTYNFLSGSSFSAAIVTGAAAILKA
                     VNKSLTPLEIETILCASADKLTDEGRNNYYGYGKLNLFSALTLDLDSLIYQSEEQQPS
                     EGTSPFSFEYTDRKATLSCGLSYGLWLDQNNKVMSAGDSSFGKTDVAQWSDIVSVATG
                     DSFSLGLKSDGTLRYTGFSGSYALNVSHWTSLKQVSAGSLHTAAVTTAGSVLTMGSNS
                     AGQCNTGIIAGVSKVECGSMHTVCLRSNGTVRSVGANFSGETNTSGWSDIVSVAAGNG
                     FTLGLKSDGTVVATGNNRFKQTDVSSWHNIIEVFAGYNYSVGLKADGTLVYTGLATEM
                     SSSLQELSNIKTLSCNRDMMILKTNDDEIIILGNTGAQERSTNKVYVYSFDDIAQDYW
                     ASTYIHHVSRLGIINGVYNGTGYIYIPHVYMTRQEFFKVILETMSIPANVDADIEFFE
                     DYEDVDSWSLPYIKTAYYYGYLTGSTDDEGKVYIRPKEPVSRAEAAVIMGRVIGLTES
                     PDMEYTDKDTVPLWAYDAISYFSHMKVLNGYPNGTIAPNDNLYRSEAAAIICRLYSYV
                     LNAYTFNHVDNTAETAD"
     gene            23092..24393
                     /locus_tag="PLF14_07010"
     CDS             23092..24393
                     /locus_tag="PLF14_07010"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOT71075.1"
                     /translation="MRKRTFLTISILLVAVILSSCTPVETPTVQPTSVPSETPPQTSI
                     SPQPPASEQPTETRPAEAAPKTATLKLSATGDVMSHYTQILDAYNAETGEYDYSNSLS
                     RIKNFTKTSDLLIANLETSMAEGEYTGYPIFNTPPALAKNLKDIGVDIVSTANNHCLD
                     KGFKGLVSTIENVKAAGLLQVGTYAAQQDRDESHGVLVKEVNGISMAIVNYSYGTNVI
                     RIPAGKEFCVNLLYEDYLGPYSILNKEQISSDLEYARSLDTDLIIALIHWGTEYVTTE
                     NEKQRQAAQFLFDNGVDILIGNHPHVLQPMGIQTIRTPEGQEKQVFVCYSLGNFMSNI
                     TRTHYTLTSAILNLYIEKNLETDVTVIKGYDYVPTYMLTPEWGGSRPYVLPVHDAIKE
                     YENGSSTLINEKIYKDLLASLKICHDILGRDYDYYYIKRTD"
     gene            complement(24395..26218)
                     /gene="typA"
                     /locus_tag="PLF14_07015"
     CDS             complement(24395..26218)
                     /gene="typA"
                     /locus_tag="PLF14_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOT71076.1"
                     /translation="MIREDLRNIAIIAHVDHGKTTLVDAMLKQAGVFRKNQVVADRVM
                     DSGDLEREKGITILAKNASVRYKNTKINIVDTPGHADFSGEVERVLKMVDGVLLLVDA
                     FEGPMPQTRFVLSKALELNHKVIIVVNKVDREGADIRKTENDVLELLMELQANDTQLD
                     SPYVYCSAKEGAASLNPEIKGTDLSPLFDTVLQHINPPEGDENRPLQVLISSIDYNDY
                     VGRIAIGRIERGHIKANSDVAVVNFHNPEEPHRAKVTNIYSFEGVKREPVEEAYVGDI
                     ISFSGIPEISIGDTICDPEFVEAIEFAKISPPTIEMTFSVNDSPFAGREGKYVTSRHL
                     RERLFRETLKDVSLKVKETESADAFIVMGRGEMHLSILVETMRREGYEFQLSTPRVLF
                     TDIDGVKQEPMEHVYIDVPSDCVGAVCEKMGRRKGEMLCMNLSGARMKMEFSVPARGL
                     FGYRNEFLTDTAGEGIMNTLYEGYAPYKGEIGYRSNGSLIAFETGTAVTYGLYNAQER
                     GVLFINPGEEVYAGMVVGETPKDEDITINVCKKKHVTNMRAAGSDEALRLSPPKKMSL
                     EQAMEYIKEDELLEITPLSMRIRKKILDPVMRMRAKLKDKQ"
     gene            complement(26352..26894)
                     /locus_tag="PLF14_07020"
     CDS             complement(26352..26894)
                     /locus_tag="PLF14_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71077.1"
                     /translation="MPTVTKKKSRWTIAVIVLILSVAVLLFFVIRANITQGRGKSVDT
                     FSTYEDLLEKSPVAVIATVVSDNKEFVYDDIIFAVTEVEIKDCLRGDLGNLTLEILQT
                     KMKENPYLIKNTEVLLFLREYKGPVAENVYIINGLHNGQFIIKDNTLNEVRAGYTTTI
                     LKTADLESVITDIKNTEYKE"
     gene            27063..28382
                     /gene="melA"
                     /locus_tag="PLF14_07025"
     CDS             27063..28382
                     /gene="melA"
                     /locus_tag="PLF14_07025"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOT71078.1"
                     /translation="MKKFAFIGGGSFGFTRNLVKDLLTFPAFRDAHIVLMDIDPERLD
                     YIHQAVRKIIKAGKYPAKVTATTDRVKALKGADGVLCTILSGDVDVWQHDILIPKKYG
                     VDINIGDTRGPAGIFRFLRTVPVMLDICKDIEKYCPDAVFLNYTNPMAMLCMAMQKKF
                     PNLKITGLCHSVQGTAGMLAKWIGAPMNEITYKCAGVNHQAFYLEYKWNGKDAYPLIR
                     EAIKDPEIYQKELVRNNMFEHLDYYVTESSGHNSEYVAWYRKNPELLEKYCMNGTNWN
                     PGHYAYILNEYLKRKDTWRDSIKEWLAKEDINLERGYEYATYIFNAVFGDNTMFKFNG
                     NVMNHGIIENLPYDCCVEVPILASRNSLEPIHIGAMPDQLATLINTTARCEVMAVDAA
                     LERNPRKVFHAVAFDPLTSAVLSLDQIKEMVDEMMEKNKEYLGYLYK"
BASE COUNT         8554 a         5803 c         5847 g         8304 t
ORIGIN      
        1 ccttgagttt ccgtactttt tcacctaccg atttaaacat ctgtgttttc cattgaaaat
       61 tgcgtaaaaa agctccaaaa atgaagaaat cctacctctt ttgtggtaaa atttaattac
      121 caaaaaaatg ttactaaaca aaagaaagaa aggatctctc atggcaaatt atactacaaa
      181 aacttactca atgaaacggg aaattgttaa tttttctaag gatttgacga aaaatcttcc
      241 tcttcccgta aaaaagttca taactgatat gaactacgga atgttagcgt caaacagttg
      301 tctgctttcc gacattgcag atactcttca tgaagaaaca aaaaagaaga acactataga
      361 acgtttagct cttaatcttg aaaaaggaat accggacaag ttaaacagaa attaccttaa
      421 gaaagtaaaa aatattgttg gcaaagaacc ggtagtacat attgacgata ccgatatagt
      481 caaaccatgc ggacgcaaat ttgaggatat gggaagagta agagacgggt ctgaaagttc
      541 ttcagagaag aatgtttaca agaatggata ttatgttacc gaagcatgtg cacttacaac
      601 aaacaatcat cctgtcagtg tattctctaa aatacattca tcacgtgaag aggattacat
      661 ctctacgaat gccataacat ttcaagccat agatgaatct gtaaagttgt tcaaaaaagc
      721 aacatttgta atggatagag gatatgacgc agataaaata ttctttaagc ttgaaaaatt
      781 acggcaggag tacatcgtaa gattgacggt aaagcggaaa gttgtgcgca ataacaggaa
      841 gatttccgtt tccgagctgt gtaatcgcag aaaaggcaag ataaaaataa cagtcaggta
      901 taacgacaag gattatgatg catatttatc acatgtaaaa gtacagctta aggaagacag
      961 caactatgta tatttggtat tagtatatgg agtaaccgaa caccctatga tgcttcttac
     1021 caacaaagag ataaaatcaa aagaagatgt tattcgcata gcaagacttt acttctccag
     1081 atggcgcata gaggaatact tccgttccaa gaagcagata ttcggctttg aaaagttcag
     1141 agtgagaagt atggtgtcaa ttaggtcttt gaattttatg ataacagcct gcatgacttt
     1201 ccttgcttta atgtccatga agagtgtacg cagctcacta ctaattgaga tattgcgtag
     1261 agcagcacca cttaagcaga aggtacaatt ctactattac cgtatagcca aaggtatatt
     1321 aaaaatactc tcttatgcac gtaagggtgt aagagggtgg ttcaaagtta agcgtcaaaa
     1381 gtacagacaa ttagccttca gttttttatc ctgagctata caaaattgaa tactcatgaa
     1441 aagttccgac atagtgggaa ctgattttca tacacaaaac tccggtttta gagactttta
     1501 tatgcaattt tactaatcat tcctctatag tcttaaagct aacctcaaaa accggtcaat
     1561 tatccggttt cgagtgcatt ttacggaaac tcaagttatt cttctaccgg ataaatccgt
     1621 ttgtttttcc agccttgcta tatgctattt gtttttgcaa aaacatatga tataataaca
     1681 tatgatgtta gaatatcata aatatgtgag gtagttatgt atataaaacg acacatagaa
     1741 tttgctgttt cggagcgggc aagggaaaag ggagctgtca tagttaccgg agcacgacag
     1801 gtcggcaaga ctacattaat tgaaaatatt aaacccgata tatcgcgagt tacttttgat
     1861 gatataaccg ttcggcatcg tgtaaagaag gatcctgctg catttttgca gctaaatccg
     1921 ccacctcttt ttattgatga agtacaatat gtcccggaaa ttttccatta cataaaaatt
     1981 tctttagacc gaagtcgtaa taaaggagat ttttatctta ccggttcaca aagttttgaa
     2041 cttatgaaga atgtcagtga gagcttagcc ggtcgtgcgg gaatattaaa tctgatgggg
     2101 ctttcgttac gggaaatgag aaatgaaaac tggaatgaac ccttcatccc tactaccgac
     2161 tatctaatga gacgtaaggc tgcaaaaata gacttgaata taaatgaagt ttggaaaatt
     2221 attcatcgcg gctgtatgcc cgagttattt aaggaacaaa gttataactg gcaaaacttc
     2281 tattccgact atattaagac gtatatagaa agagatgttc gaaatctcac acaggtagct
     2341 gatgaatcgg attttatgaa atttatgact gtttgtgccg ccatgaccgg tagcctgtta
     2401 aatatagctt cccttgcaaa agaagtggga atcagtcagc cgacagcgaa gcgttggctt
     2461 tctatattga aaacgagcaa tattgtttat ttattaaaac cttactcaaa caatgcaatc
     2521 aaacgagcaa taagaacacc caaactttac tttttggata caggccttgc agcatatttg
     2581 acccgctggc ttacacctga gactctttct acaggtgcaa tgaacggaca tttctttgag
     2641 acttttgttg tttcggagtt gattaaaagt tacgcaaaca gaggtatgga cgagtatttc
     2701 tattttcttc gtgacagtaa cggtagagaa attgatcttc ttatcttcca ggataataca
     2761 ctattcccta ttgaaatcaa gaaacataca aatccttctt atagggatat agcaagtttt
     2821 gacatgctga aatccgtaaa aaatataact ataggggagg gcggtgtaat atgcccttca
     2881 caggatttgc taccgcttaa ggataatcac aaaatcatac ctctgtgggc tgtataattt
     2941 aatagaattt aacaatctta ttacccgtga aacatgtttc tctcttaata tttaacccta
     3001 aacaggctat aataaaaaga ggaggcttta tgaagattct tttagaactg tattgggttt
     3061 ttttcaaagt cgggatgttc gctttcggcg gcggctatac gatgctgcct cttcttcaaa
     3121 aagagattgt ggaaaaaaag aagtggctca cctccgagga aatcattgat tactataccc
     3181 ttagccagtg ctcgccgggc attatcgccg tcaatgtttc cacatatata ggtttcaaaa
     3241 agaagcgtta tctcggaggc atagtcagcg ccttgggtgt tgcaactcct tcaattataa
     3301 ttataacggt aatagcggcg gttataacaa attttctgca tataaaagaa gtctcttatg
     3361 cctttgcggg aatcagggta gccgttgcgg cacttgttat agttacaata gtggacatgt
     3421 ataaaaaatc ggttgtggac tggctttgca tcatacttta catcacttcc ttccttttgt
     3481 cggtacttac ttcgatatca cccgtattca tagtactggg cgccggcatt accggactgg
     3541 ttctgggaac aagaatgcgg gaaagaaaag gagataagaa tgatatatct tgagctgttc
     3601 tgggaatttt tcaagaccgg ccttttctcc gtaggaggag gtcttgcaac aatacctttc
     3661 ctttatgata tcggaaggag atatgactgg ctggacacat ccataatccc cgatatgata
     3721 gctatatccg aatccactcc gggtcccata ggaataaaca tggcaactta tacgggctac
     3781 accgctgccg gcattttagg tgcattgatt gcttccatag gggtaattct tccttccgtc
     3841 attattgtcc ttctgatttc gaggtttttg gtttctttca gtcaggacaa aagagtcctc
     3901 aataccatgt acggattacg acccgcttca ttgggactta tatcggcggc agcttttacg
     3961 cttctcaatg tttcggtcat aacatatgat gcttttgttg aatccgggaa tattcttgat
     4021 ataatcaata taaaggccct ggtattgttt gtttgtttac tggtaattag actgtttttc
     4081 agaaaaatcc accctatact gtttatagtc tttgccggag taatcggggt aatatttaag
     4141 ttttaagggg gacttatgac tgacattctc agaatactct tttccgccat tgcacctgtt
     4201 gcagtactta tcatactgca tctggtaatt gacagacatg acagagagcc tgcaaaactg
     4261 ttaatcttac agtttgttgc gggatgtctt acaagtattc ccgcaatatt tattgaaaac
     4321 ttcctggaag ggctgaacat cttctccgga accattctgg gggtagcata tacctcttac
     4381 atagttgcgg gacttacgga ggaatcgctt aaaagagcat ccgtgtatct tgtttcctac
     4441 cacaagcccg cctacaatga aaaactggac ggaatcatat actgttgttt tgcatctttg
     4501 ggatttgcag cggtggaaaa tattttatat atagtgaatt tccaggcgga aaatccttcg
     4561 atagcccttt acagaggaat tttctccgtt ccggggcaca tgctttttgc ggttacaatg
     4621 ggatattaca tatcgttatc caaatttgct ccgacatatg ctctcatgag aagttacaga
     4681 gtaaagtcat tattgtaccc cgttctgctt cacggtacat tcaacttcat actttcgttg
     4741 aacattcact ggcttatagc atttgttcct tttgtaatat tcttatggat atttaatatt
     4801 aaaaaattgc gttctttctc aaaagaaagt cgttattaca aaaacttata cgacaacaat
     4861 aaccccggta acacaagtat ttagcttatt tttgcgcgaa aaaaagcatc agacatttga
     4921 tgctttcttt atatttattc agattcttct actttttaac ggatacaacg aacttaacta
     4981 cttcttcgct tcctgccaat tccacaaagt tttcatccgg agcattgtat tgaactataa
     5041 aaagttcata tccgtcttca ataatgcctt cccaccagtg gatgcattcg ctcttcttca
     5101 tcttggcctt cctgataaat ttgggtaatt catcaagatt gtcgcatctt cttactatcg
     5161 ttgcgaaaat ttccgcatat ttggaatttt caaccggcat cttatacttg cctgcagcta
     5221 tatcagccaa cgttttatct aaatccttta ctgccaatac atatagttct ttttttcttg
     5281 ccatgtatag atcctcctat tactgtcaat cttaactaat tatatctatt ttagccaaat
     5341 atgtccataa aaatattcca taaatttagg tgcttctatt tgtatgattt atacagttag
     5401 ttgcattttg agtatccgca gttcctgcaa atcatgcatc ctccgtcact ttccagcgga
     5461 gctttgcatt cgggacaaag tctgctctca aaactctttt cattcttatc cgcagaattc
     5521 tttgtttctc cgtaatgcag atagggaacg gtcctcttta cttcctcctt aagcccgccg
     5581 ttcatcttgg caactttttc tatcatcctg cctatcgcat ccggacagga caatattccc
     5641 aaatccttct gtcttatggt tgatggacat ctgatgcctt taagctggtc aataattgct
     5701 gcggtatcta tacccgatct caacgcaatg gaaaccagcc ttgcaaccgc ttccgactgt
     5761 gaaggacatc ctcccgctct cccggtattg gtgaatatct cgcatatttc cccgttctca
     5821 tcatagttca ctgttatgta cagatttccg caacccgtcc ggaatttttc cgtcaatccc
     5881 gtggtaattc tcggtcgttg cttaggtatc aataccgtac ccggctgaac cgcctcttta
     5941 tgttgttgtt tcggtatgtt cagtacctgt gcggctctgc ttccgtctct gtataaagtg
     6001 actcctttgc atcccaacgc atatgcctgc ataaatactt cccttacatc gtccaatgtc
     6061 gcttcattgc gtaaattaac cgttttggaa accgcattgt ccacatgctc ctgaaaaatc
     6121 gcctgcatac gtatatgcca tgaaggatct atgtcatggc ttgtaacaaa aacttcctta
     6181 acatcctccg gaacatccgt catacggctg accgagcctt tttttgcaat atctctcatc
     6241 agctcttcgg aatagaaacc tctttttctt gccacttctt caaatacggg attgatttcc
     6301 accatttcgg tattgtccat tatattgcgt atgtatgaaa tggcaaaaac aggttcaatt
     6361 ccgctggata cgcctgctat tatgcttaga gtaccggtag gagctattgt ggtggttgtg
     6421 gcatttctca cctttatatc ctgcgcggga tagacgcttt tatcatatgc cgggaatact
     6481 cccttaatct ccgcaagact cattgaggcc tttttggatt gctcgtttat gaacttcatt
     6541 acctcatatc ccagttccaa tgcctgctca ctgttgtacg gaatccccat tgtatacagg
     6601 ctgtccgccc atcccataac acctaatcct atcttcctgg tggcctttgt attctttgct
     6661 attttctcat tcggataaat atttacatca attacattgt ccagaaaatg cacggctttt
     6721 attacacatc tttccagttt ttcatagtca aaaccgctct tatcttcttt aatcattgtt
     6781 aacagattaa tagaacccaa attgcatgat tcgtacggaa gcaacggttg ttctccgcag
     6841 ggattggtgc tttctatttc tcccaattcc ggtacggtgt ttttcttgtt tattctgtcc
     6901 agaaaaacta ttcccggttc cccgttcaac catgcatgct ccactatcat gtcgaaaatg
     6961 tctttggctc tctccattcc taccgttttc tttgtcgaag gatcaataag ctcgtacata
     7021 ttgtcatgtt ccaccgcctg cataaactgt tcggtcaagc ccacacttat attaaagtta
     7081 tttaattcgg tgctgttggc tttgcaggtt ataaattccc ttatatcggg gtggtccact
     7141 ctgagaatac ccatattggc tccccttctt gttcctccct gcttgaccgc ttcggttgcg
     7201 atattaaaca ctttcatgaa actgatcgga ccggaagcta ttcctccggt ggtgctgacg
     7261 gcagcccctt tttgccggat tctggaaaaa ctgaaaccgg ttcctccgcc gcttttatgt
     7321 attaaagccg cattctttac cgtttcaaat atggcatcca tggagtcttc gatgggtagc
     7381 acaaaacagg ccgacaattg gcctaacggt cttcccgcat tcatcaatgt gggtgaattg
     7441 ggtaaaaact ccagatttat catcatatca tagaaatctt tttcggtttt ctctatatct
     7501 tctttaccca ggtaatcgga gtcggcggat gctatcgtct ttgccaccct cttgaacata
     7561 tcatcataag attctatgac attaccttcc gaatcctttt taagatacct tttctccagt
     7621 actttttttg cattctccgt aagcatgact attcctctgt aaactatatt ttgtgttacc
     7681 aatacatatg gtgtgctata tattgtatca tcgtgcgttt ctctgtcaac gaatttgacg
     7741 gaattctttt gtaaattttt tttcgctctt ttcgcatgtt catagtattt tactatttat
     7801 atgatataat ttatctgcaa gcagaaagag ggaaaactat ggaagtaaga cataaatcag
     7861 ccataccgat atatattgcc ggtgcagtat gggtgctgta tagcataatt ttccctatgc
     7921 ataaacttat tcatctggta atcgcaaccg tactgtttat agtcaccttt ttcttaagca
     7981 ggagattttt cccggacagg attgagatgg tggaagagcc tataaaatat accggcgcaa
     8041 aggatgcgga cctggctatc gcagaaggaa acagatacat tgaggagatg agaaaagcca
     8101 atatccgtat acaaaacgct gtagtgtcgg aaaagattga catactcagc gatgttataa
     8161 aaaagatatt cgcatatatc acggagaaac cggagaaggt caaggatata cgcagactta
     8221 tcacatattt tctgcctacc acggttaaac ttctgaatgc ttatgcaaga gcggaagaac
     8281 agaacaatca cggagagaat ataacgcagg taaaaaccga tattgagggg gcaatggacg
     8341 gaattataca ttccttccgt aagtaccttg ataatctgta tgccgacgag gctatggaca
     8401 tagacgctga aataactgtc tttgacagta tgttgcaagc ggaaaattta aaatagatga
     8461 aaggataaca gcaatgaacg aaattacact aacagagaaa gacagagaac ttgcactttc
     8521 atacggagag aagctggata ttaaaagcag cgcttccatt cttcaatacg gcgccgatgc
     8581 gcaaagaaag ataacaacct tctcggatgc cacattggaa aagatacgtt ccaaggaact
     8641 gggagagata ggggatatga taggcaatct catggcacag cttaaggact ttaccgcaga
     8701 ccgggaatcg ggaaacttct tggagagact cttcagaaaa acaaaaaggt acattgacgt
     8761 actgaaagca cgttacacca aagtcgaagc aaatgtaaac aagattgtgg ctgtaatgga
     8821 agatcatcag gtagtgctga tgaaagatat tacaatgctg gacaagatgt atgaaatgaa
     8881 tctggattac attaaagaac tcgctttgta tatagaaggc ggcagagcca agcataaaga
     8941 acttaaagag acaaagcttg tggagctgag aaaaaaagct aaagaaacca atcttcccga
     9001 agacgctcag gcggcaaatg actttgccaa tatgttagac agagtggaaa agaagatata
     9061 tgacctggag ataacaagaa atattgccgt tcagatggct ccgcagataa ggctgataca
     9121 gaacaataac actcttctga cggaaaagat acaaaccacc cttataaaca ccattcctct
     9181 ttggaaaaac cgcatggtac ttacattaag catcacacat tcgcagaatg ccatgaaagc
     9241 tcagcgtgcc gttaccgaca tgacaaacga actgcttctc aagaatgcgg aaatgctgaa
     9301 acagagcagt gtggatattg ccaaggaatc ggaaagaggc attgtggata tagaaacact
     9361 gaaagccacc aatgaaagcc tcattaccac tcttgaggag attctccgta ttcaggaaga
     9421 aggaaaggca aagaggaaag aggctgaaaa agaacttgac aggattgagg aacagctcaa
     9481 gagcaaattg cttgagttta agaactgatg aaatttaagg aaaacagagc attagcccgg
     9541 atattggtag taattgcagt aatcgcttct gtgcttataa gcgggcatgt atctctttct
     9601 tcgcaaagaa gagcggttat gaactctttt tacgacacaa tggatgccga tctgaacacc
     9661 aagtcggctt atgcggataa tctgtccgga gtggcatcaa gataccttga ccgcaacagc
     9721 gaatacataa caaatatggc acaggccaga gacctgctgt tagaggcaaa gacacctgcg
     9781 gaaaaatatg cggcatcggt aaaaataacc aatgccgcag cggctcttta tgatgtatta
     9841 ggcacaatga gtctcaatga aaccgatgag cggttaagac gaagcaatta tgccgatata
     9901 gtggccgtgg atgatatctt aaagagaaca tcgttcaatc aggatgttga taaatttaac
     9961 agtcaattgg ccctgtttcc ggctaatgtt attgcctcaa taaccggcat tgacaaagcg
    10021 gaatatttcc gatgaacttt tatactgttt tcccgttata ttaatatgaa gaaacttgcg
    10081 atattcatta cattaatatt gattctgacc tgtaatgcgg tatttgccgc agatattcct
    10141 gcgcctactt ccgatttcta tgtcaatgat accgccaatg tgttatccga gaagaccaag
    10201 aaaacaataa tagataaaaa tcttgcactc tacgaaaaga cgggagctca gattgttgtt
    10261 gttacggtag aatatactca tacagcatcc attgccgatt acgcatacga gcttttcaat
    10321 gaatataaaa taggttccgc cgaaaaaaac aacggtctgc tgttgcttct ttccatagga
    10381 gatgatgact attatgttct gcaaggggaa ggcttggaga gaacgctgtc cagcggtact
    10441 atctcttatc tgctgtacga acatctggaa ccgtattttg ccaagcaaca atatgatgag
    10501 ggagttttaa acattttcaa tgtattatac aaagagatgt tggcaattca cgatacaacc
    10561 atagaatatg aggacgtata tccgccgcaa gacaattatt actatcctga tgattacttt
    10621 gagcaggagg attcttcctt ctccgttttc ggtttctttg caactatttt tgctattatg
    10681 agggttatct tcttcttcat ggttattttc ggagtgcttt ccgcaatatt cggaagaaga
    10741 aaaagtacaa ggcgatccga tgttgcccgt aatatactgc cgattataag cactttaggc
    10801 tatatgaata cactgaaaaa tatgaacaac acaagtaact attcttcctt caacaataac
    10861 agcagcaccg tcaacagaag tagttactct tcaggcagaa gttcctactc gggaggaggg
    10921 tcttctttcg gcagcagttc ttcttcctcc agaagttcct tttcttccgg cggcagatcg
    10981 ggcggtttca gcagcggcgg aggcggttcg tccagaggag gaggagcagg aagaggtggc
    11041 agatagccgc cttattattt cctccgtata aaaactttac ttattataaa atattgacct
    11101 tgattcttat tcttcggttg caccggagtc ctttttcttt ttgttcttaa atatttttga
    11161 taagagcaat attgccaggt atataattgt aagtgctata aaaacaccta acattccgaa
    11221 aagaaccaca cgaggtgtct ttaaaaaatt atctatatca aaatgtatat tcattttccg
    11281 cctccgttat atcagttttt ccaccagggc aatcattacg ccgccggcaa ctaccgatgc
    11341 aatctgaccg gctacgttgg ctccggccgc atgcattaat aagaaatttg taggatcttc
    11401 ttttaatccc attttatgta ccaccctgga ggacatgggg aatgccgata ttcccgccgc
    11461 acctaccata ggatttattt tcttcttgac aaacagattc agaagtttgg caaacagcac
    11521 tccgcctatc gtatcaaata cgaatgccac aagtcctaaa agaatgataa ccagagtgtc
    11581 tacctttacg aaaagatcat atctcatctt ggaagctatg gttattccca acagtatggt
    11641 tacgagattg gcaagttcct tctgcgccga ttcggataat attctcaaga ccttgcactc
    11701 tcttaacaga ttaccgaaca ttaagaatcc taccagtgct accgatctcg gtgccagaat
    11761 acctactatc attgtggtta ctataggaaa cagtattctt accgttgcgg atacttcctt
    11821 ctgctcgtaa ggcatcctta taagtcgctc ctttttggtt gtgaaaaggc gaataacggg
    11881 aggctgtatt ataggaacca atgccatata cgaatacgcc gccactataa tcgctcctat
    11941 gtagttgctg ttaaagtagt tagcaacaaa tatggatgtg ggaccgtcag cagctccgat
    12001 aatgcctatt gatgcggcat ctttcagatc aaatcccagc aaagcagcca aggcaaatgt
    12061 aaagaatata ccgaactgtg cagccgcacc gaacagcaac attttaggat tggataaaag
    12121 cggtccgaaa tctatcattg ctcctattcc gataaacaga agcaacggaa acaactcgtt
    12181 agctattccc gcattgaaaa ggacatccaa aacgccttct tccgttttac cgttgataac
    12241 ctgcgttatc gctcccgatg tgggaagatt ggcaagaatt gccccgaacc ccataggcag
    12301 aagcagtgtg ggttccatgt ctttggctat agccagccaa atcaaaaccc ctcctatcac
    12361 ccacatgagg accatctccc atcttatctc cagtacatta gaaaaaatat cagccatgat
    12421 tatctcttcc cccgattttt acaggattaa gcacctgttc atctttagta tatataatac
    12481 ttttttcctg ccgcttgcgt caagtgatta gttgcattaa accgtttgta tatatatact
    12541 ttactgggaa aggaattgta aaaatgaata tagaaaacat attatccaaa gtggaccata
    12601 ctcttcttaa acagacggct aagtgggaag atattaaaac gatttgtgaa gatgcgatta
    12661 aatataattg tgcgtcggcc tgtattcctc cgtactttgt taagcaggca aaagattgtt
    12721 ttaagaataa ccttaaaatc tgtacggtta taggttttcc caacggatac agcaccacgg
    12781 caaccaagtg ttttgaaact gcacgggctg taagcgacgg agctgacgaa atagatatgg
    12841 ttctcaacat aaacatgctc aaagacaagc tttacgatga aatactgacc gaaattaatc
    12901 aggttaaaaa agcctgttcg ggaaaacttc ttaaagttat aatcgaaacc tgtcttctga
    12961 ccgaagagga aaagataaag atgtgcgaaa taatatcaaa aagcgacgcc gactatataa
    13021 agacatcaac gggcttttcc acagcaggcg ccacgagaga agacattatg ctttttaagg
    13081 aacatattaa aaacggcaag ctcataaaag ccgccggagg cataaggtct ttaaacgatg
    13141 caaatgattt tatcagtctc ggctcttccc gactgggcac atcaagtata gtaagaattg
    13201 ttaaggatga ggaaaataca agttattgaa atgttaaaga aatgtgtcaa gaatgtgaac
    13261 tttttgcttt ttcccccttg tttataaact ataaatctga tagtatttat atgtgaattt
    13321 cattatatca aaaaaggagt ataattatga aaaaactatt tagtatcgta ttggtagtgg
    13381 tgcttttgct ttctatgctg tccttagcag gctgccaagc taaaacctat gagctggcaa
    13441 tgattaccga cataggagac attgacgaca agtcctttaa ccagggaaca tgggaaggat
    13501 tgagggacta tgcggtagag cataaaatta cgcataagta ttacaagccc accgaagctt
    13561 ccaccgatgc atatattgcg gctattgatc ttgcagtatc atccggagct aaagttgtcg
    13621 taacaccggg attccttttc gaagaagcta tatacatagc tcaagataaa tatccggaaa
    13681 taatgtttat tcttcttgac ggagtaccgc atgatgcaaa ttacgagaac ttcaaaaccg
    13741 gtcccaatgt tttccctgtt gtatatgcgg aagagcattg cggttacctt gcaggctatg
    13801 ctgctgtagt agacggatac agaaacttgg gttatcaggg cggaatggcc gttccggcag
    13861 tttgcagata tggattcgga tatgtacagg gtgctaatga tgcagccaga gatttgggcc
    13921 ttgcaaaagg atcaattaaa ataatgtaca actattccgg agactttgcc gatacaccgg
    13981 catcacagac aagagctgca ggctggtatc aggcaggaac cgaagttatc ttcgcagcag
    14041 gcggaaaagt aggaaactcc gttatgtcgg cagcggaagc agccaataaa gcggttatag
    14101 gtgttgacgt tgaccaatcg gcggaatcgc ctacggttat ctcttcggca atgaagggat
    14161 tggcaacaat ggttcagtac ggattaaaag ggttctatgc aggaaagttc ccgggcggag
    14221 aagccgtaac ggtcggtgca gaatccaatg ctgtaagcct ggcaccgttc tcaacttcaa
    14281 aatggaaaac attctcacag gccgattatg acgcattatt tgcaaaattg gctaacggaa
    14341 ctatagttcc ggaaaaagat ctcgacagaa caaatcccac ggaactcaaa ctcgattatg
    14401 ttaaattaac ttttgtagag taagagtaaa acaataaaga aacaaaaact gccgtataaa
    14461 agcggcagtt ttttatcttg taatactata tatttctgtt ttttcacttt agcggttatt
    14521 ccgtatttat tattcttttc aaatattata taataaagta agttaaaaac acttaaagcc
    14581 aaggagcaaa tttatggatt acatcatcga gatgttaaat atcaggaagg aatttcccgg
    14641 aattgtcgct aacgatgata tcactctccg aattaagaaa acggaaatac atgcactttt
    14701 gggtgaaaac ggagcaggaa aaagcactct tatgagcata cttttcggtc tttacaaacc
    14761 ggaggcgggt gtcataaagt taagaggcaa ggaaattaat atttccgatc cgaaaacggc
    14821 aagtcatctg ggcataggaa tggtccatca gcacttcaag ttggtggaag attttacggt
    14881 attggaaaat ataatactgg gtgcggaaac ggtaaaaaac ggctttctta aaatggatga
    14941 agcaaaaaag aaagtaatgg aactgagcaa ccgttacaag ctgtatatag atcccgatgc
    15001 aaaagtgtct aacattaccg taggtatgca acagcgggtg gaaatactta aaatgttgta
    15061 tagagagaat gaaatactca tctttgacga acctacggcg gttcttactc cccaggagat
    15121 tgatgagttg atggatataa tgaaagagct gtccaaagaa ggaaagtctt tgatattcat
    15181 cactcacaag ttggaggaaa ttaagaaagt tgccgacaga gttacagttt taagaagagg
    15241 aaaatgcatc ggtacctata acgtagaaga tgtcacaaaa gaatcgttga gtgaaatgat
    15301 ggtcggaaga aaggtacagt ttacggtgga caaaaaagat attactcccg gtgaaaccat
    15361 ttttgaagtt aaaaaccttt ccatgaagac taaaggttcc acaaagaata ctcttaataa
    15421 aatatctttt aaggtaagaa aaaatgaaat tgtctgcata gcgggaatag acggtaacgg
    15481 acaaagcgaa ctggtttatg cacttacggg tataagacat tttgatgagg gacagatact
    15541 tgtccacggt gaggattgga ccaagaagag tatacgatac agaaattccc acggaatgag
    15601 ccatatcccc gaagaccggc ataaatacgg acttatactg gaatacagta tggcatataa
    15661 ccttatactt cagacttatt tcaccgacag atttcaaaaa agaggttttc tcaagaatga
    15721 cagtatacat gaatatgccg acggtttgat taaaaagtac gacatccgaa gcggacaagg
    15781 tgcacagacc gttaccaggt ccatgtcggg aggtaatcag cagaaagtaa tattagccag
    15841 agaaatagac aaagaccctt ccataatgat agctgtccaa ccggcaaggg gtttagatgt
    15901 cggagcgacg gaattcatac acaagaaatt ggtagagcaa agagagagcg gaaaagcggt
    15961 tttacttatt tcgctggaac tggaagaaat aatgaattta agtgacagaa ttcttgttat
    16021 atacgaagga gagttggtag gcgatcttga tcccaaaaag accactatac aggagttggg
    16081 cctgtatatg gcaggagcaa agagagatga ggtgaaagca tgagtaaccg caattccatg
    16141 aatcagtttt ttgacagatt aagcagaaaa aaagggacgg gaagctttat ttcctcttta
    16201 gtagctatag gaataggcct tataataggc cttataatcc ttctcatatc caatccccgg
    16261 gaagcctttg caggattttt taccatgctt atcggaggtt ttaacgacgg gacaagaagc
    16321 ttcggaaacg tattctatat agccacacct atcataatga cggggctggc ggtgggattc
    16381 gcattcaaga ccggcctttt caatatagga gcaagcggtc agtttatcgt aggggcatat
    16441 gttgcggtac ttatcggagt aaaacttcct cagctgggag gcttccagtg ggttttgggc
    16501 atattggccg ccatggctat aggaacgata tggggagctg taccgggact tttaaaggct
    16561 tatctcaatg ttcacgaagt aatagcctcc attatgatga actacatagg catgtttatg
    16621 gttaactacc ttataaaaca aactgtgctc aatgtatata ccaacagatc gcttgccgtg
    16681 gcaccgaccg caaatatacc caagatgggt cttacggcaa tatttcccgg ttccagtatt
    16741 aacgggggat ttcttatagc gatattgttt gtaattctca tgtatataat tctggaaaag
    16801 accaccttcg gatatgagct gaaagcctgc gggcacaaca gattcgcagc taaatatgcc
    16861 ggtataaacg aaaacagaag cattatactt tccatggcaa tatccggaat gcttgcaggt
    16921 gtaggcggag ccttacacta tttgtccggt gcgggcgtat atatcgaggt cgtggatgtt
    16981 ctggccgctc aggggtttac gggaatttcc gtttcacttt taggcatgag caatcccata
    17041 ggtgtctttt tcgccggtat atttattgca tggctgcaga taggcggttt ctatatgcaa
    17101 agatacagct ttgttccgga gttcatacag attataacgg caattatcat ctactgttcc
    17161 gcagcatctt tgcttattcg gaactatctg gaattgcggc aacttaagaa agacgaaaac
    17221 cttaccgccg attccgatgg tgacacggca gatattgtta atgttgaaaa tcaaggaggt
    17281 gacaagtaat ggatatgcta tatcttttac tcagacagac catgtatttc tctattccgt
    17341 tacttatagt ggctttagga ggcatgtttt ccgaaagaag cggcgtaacc aatatagcgc
    17401 ttgaaggtat catggtaatg ggaggattta ccggcatata ttttatcaat caaatgcaat
    17461 cacaaatgag cggacagtgg ctgcttgtct tagctatttt catagccatg gcgggaggtt
    17521 tgcttttatc tcttccgcat gcatatgctt cggttaatct taaagccgat caggtcatat
    17581 cgggaaccgc ccttaacatg tttgcacccg ctttcgttat ttatatagcc agaatgattc
    17641 agccttcggg aatagccaat gtttctttta aaaacacttt ctttatacac gaagtacctg
    17701 tgctttgtga cataccggta ataggtgata ttctcttcaa gaatacatat ataaccacat
    17761 tcttaggctt tttcatactt atagtttccg caatagttct gtacaaaacc cgtttcggaa
    17821 tgagattgag ggcttgcgga gagcatccgc atgctgccga ttcggtagga ataaatgttt
    17881 acaaaatgag atatataggt gttatgattt ccggagtgct tgccggactg ggcggacttg
    17941 ttctgaccgt tcccatatcc accgaattta agggagccgt taacggatac ggttttctgg
    18001 ctttggccgt tctcattttc ggtcagtgga aacctatgag aatattgtgg gcttctttgt
    18061 tcttcggatt tatgcagact atatcggcag cttattcggc aatacccttc ctgttgaatc
    18121 tgaatatacc ttcggaattc ttcaagatga ctccgtatat agctacactt atagtgcttg
    18181 cattctcgtc gaaatccagt aaagctccga aagctgtcgg acagccgtat gaccaggggg
    18241 ccagataggt catgagaatg tatgacatca tacaaagaaa aagagataaa catcaactga
    18301 cggatgagga aatccgatac tttgtaaaag gatataccga cggctccata cccgattatc
    18361 aggctgccgc tttactgatg gcaatctttc tcaacggtat gaccgatcat gaaacttccg
    18421 tactgactct ggctatggct caatcgggag atatgattga tttatcggct gttaacggaa
    18481 ttaccgtgga taagcacagt acgggaggag tgggagacaa aacttctttg atagtggctc
    18541 cctgcgtggc ttctttgggc tttaaagtag ccaaaatgag cggaagagga ctgggacata
    18601 ccggaggtac ggttgataag ttggagtcca ttcccggcta caggacctct ttagacagac
    18661 aggagtttat cgataacgta aacagaatag gaatagcatt ggtgggacag agcggtaatc
    18721 ttgctcccgc cgataagaaa atatatgcgt tgcgtgatgt gacggcgact atagattgtc
    18781 ttccccttat agtgtccagc ataatgagca aaaaaatagc ggcaggatca caaaatatcc
    18841 tgctggatgt caaggtggga acgggatcct ttacacctaa actggaagat gccgttattt
    18901 tggcgcaaaa aatggtagac accggaaagc ttgcgggaag aaacattgcc gcattgataa
    18961 ccaacatgga tgtacctttg ggaaatgcca taggcaacag catggagata atcgaagcct
    19021 gtgaaatact caaaaataag gggccttccg atttgaggga agtgtgcgtg gaactgtccg
    19081 catatatggt gtcgctttgc acgcaagatt cttatgaaaa ttgcagaaga aaagttctac
    19141 aaagtcttat ggacgggtcg gcttttgaaa aattcagaca actggtttcc gctcagggtg
    19201 gcgacgtggc atttatagat aatactgata agtttaaaaa agctttttac acacatgatg
    19261 tgttatcctc acattccggc tatataagtt caatggatac cgctaatata ggcagagtct
    19321 ctctgatgct gggtgccgga cgtgaaaaaa aggaagactc cattgatttt gatgcgggaa
    19381 tatacataca tgcaaaaaca ggagactcta ttaaaaaagg tcagcttctg gcaacactat
    19441 ataccaacag aaaacatgtc ctgcctgatg cggaaaatga atatctgagc tctttgacct
    19501 tttccgaaaa tttgccgcct ccacaaaaac ttatatacca aatagtaaaa tgatagtata
    19561 atattgggca ttggagttgt ttaggcatgg aaaaaggcaa gtttataatc gatttcaaaa
    19621 acagacagca tagagttgcg gtaatatcag tagccattgc cgttcttctg attgttcttg
    19681 cagtgcttct ttttaaggtg ttcaacaaat cctataataa ttatgaaggc aactctcctt
    19741 ccaatattat aaacaacggg cttatagcat atgaagacgg tcacctttac tacacccgat
    19801 ttgatgcagg ctccaagata ttcaggctgg atatagaaag cggtaaagaa aaactcgttt
    19861 acgcagcaaa tgcaaaatac ttaaatgtgc tggagggata tatttactat attgacggca
    19921 gcaacggaaa tataaagagg ataaccgttt cgggaaccga tgagttgctg ttgaaagaca
    19981 gcatatgctc caatctttat gtaacgaaag actggatata ctatattgac aataaagaaa
    20041 acaattccat ttacagaatg agtatgcacg actatattcc ggagaaatta gccgaggaaa
    20101 gcgcaacatc cgtttcggtt catgacgggt ttctgtactt cataaactat atggacaaca
    20161 acggcatata tcgtctggat ttggaaacat tggaagtgtc accggttctt aaacagcaca
    20221 ctatcttcta taccgtaaaa gacaatctta tctattacac aaatacaaac gatgaatcct
    20281 atatctacag catgaatctt gacgggtcgg ggaacaagcg actggaaggt gtcaagggct
    20341 ggtttcttgc aagttcaccg gatgatccgg attttcttta ctttatgtcg cctaccggta
    20401 ttttgaaaaa agtgaatctt gcatcactta atcagatgac cgtatccaat gacgaatgcg
    20461 catatataaa cattgtggaa gactacattg tatatctcaa ccgggacgac aatcaccatt
    20521 atcttctgaa caaaacatcc ggtaagagca caattttaag ataatcatcg gcattgctga
    20581 tttgttcctg atattgtatt attataatat tatgaagaaa tctgtaagga ttacatttgc
    20641 tttacttctt ataatcgctt tatgtttttc ctttgtgtcg gtcagcgcac aggaagattt
    20701 ttttgactcc agatattatt ggctgtattc ctcagccata ccttcggcct ggaaacttct
    20761 tgccgatatt cccacagaac cggtcacggt agcggtaatc gacacgggaa tcgagtcgga
    20821 taatcctcac ttaaaagaca gaatagatcc gcggggacgc aattttgttc cttctcctcc
    20881 ttccgacaat gttgaagaca aacaagggca cggaacggca atcgccggca tcattgccgc
    20941 cgttcccacc gaagagaaca attattacgg agtctgcggc aatctggatg ttaaaattct
    21001 tcctttaaaa gtatatggtg atattaccgg cacattacag atgcgatata taatagatgc
    21061 catttattat gctattgaga tgaatgcgga tgtaattaat attagtatcg atatccccaa
    21121 ttttgacgaa ggtctgcaaa tagcctgtct ggccgcatat gagaaatcca tccccataat
    21181 agcctcagcc ggcaataaga gcagtgagta ttactcttat ccttcttcct gttacggagt
    21241 aatatcggta tcggctcttt cattgtacga tgatgattac tttttatcct ctccgttttc
    21301 ttcctataac gaccaggtag acttatgtgc ccacggagaa gatatttaca caataggatt
    21361 aaacggaaca tataattttt taagcggctc ttctttttcc gctgcaatag taaccggtgc
    21421 ggcagcaata ctcaaagcgg ttaacaagag ccttaccccc ttggaaatag agaccatatt
    21481 gtgtgcaagt gcggacaaac tcaccgatga gggaagaaat aattattacg gttacggtaa
    21541 gctcaattta ttctccgcgc ttactctgga tttggattct ttgatatatc agtcggaaga
    21601 gcaacaaccc tcggagggca cttctccttt ttcttttgaa tatacagacc gtaaagcaac
    21661 attgtcctgc ggtctttctt acggattatg gctggaccaa aataataagg ttatgagtgc
    21721 cggtgattct tctttcggaa aaaccgatgt tgctcaatgg tcggatatag tctccgttgc
    21781 tacgggagat tccttctctt taggattaaa aagcgacgga acattgcgat ataccggttt
    21841 ctccggctcc tatgctttga atgtgtccca ctggacatcg ttaaagcagg tttctgcagg
    21901 ttcgctccac actgcggcgg ttacaacagc aggttccgtt ttgactatgg gaagcaacag
    21961 tgccggccaa tgcaataccg gtattatagc gggcgtttcc aaagtggaat gcggttcaat
    22021 gcataccgta tgcctaagat ccaacggtac ggtcagaagt gtaggtgcaa acttttccgg
    22081 tgaaaccaat acttcgggtt ggtcggatat agtttccgtt gctgcgggaa acggtttcac
    22141 actggggtta aaatccgacg gaactgtagt agcaaccggc aataaccgct tcaaacaaac
    22201 ggatgtctca tcatggcata atattataga ggtctttgca gggtataatt actccgtggg
    22261 acttaaagcg gacggaacgc ttgtatatac aggattagca acggagatgt cgtcttccct
    22321 gcaggaatta agcaatatca agactctctc ctgcaacaga gatatgatga ttctcaaaac
    22381 aaatgatgat gaaattatta ttctgggaaa caccggtgct caggagcgtt cgacaaataa
    22441 agtttatgta tacagctttg acgatatcgc acaggattac tgggcttcga catatataca
    22501 tcatgtgtcc cggttgggta ttattaacgg cgtatacaac ggaaccggat atatatatat
    22561 tcctcatgtg tatatgacca gacaggaatt cttcaaagtt attctggaaa ctatgagcat
    22621 tcctgcgaat gttgatgcgg atattgagtt ttttgaagat tatgaagatg tggattcctg
    22681 gagtcttccg tacattaaaa ccgcatatta ttacggatat cttacaggaa gtaccgatga
    22741 tgaaggtaag gtgtatataa gacctaaaga gcctgtttcg agggcggaag ctgcagtcat
    22801 aatggggcgt gtaataggtt taacggaaag tcccgacatg gaatataccg ataaagatac
    22861 tgtgcccttg tgggcatatg atgcgatttc ttacttttct catatgaagg tactcaacgg
    22921 gtatccgaac ggtactattg cgccgaatga caacttatac cgttcggaag ctgccgctat
    22981 tatatgcagg ctttacagtt atgtgctgaa tgcttatact tttaaccatg tcgataatac
    23041 agcagaaact gcagattaga cacacaacat gaaggaagaa gagggagcaa agtgagaaaa
    23101 agaacatttt taactatatc gatactgctt gttgcggtta tactgtcttc ctgcactccg
    23161 gtggaaacac ctacagtaca accgacttcc gtaccttccg aaactccgcc tcagacttct
    23221 atttctcccc aaccgcccgc ttcggagcaa ccgacggaaa cccgacctgc ggaagccgct
    23281 ccgaaaacag ctactctgaa actgtcggca acaggcgatg taatgagtca ctacactcag
    23341 attttagatg catataatgc cgaaaccggc gaatatgatt attccaattc tttgagtcga
    23401 attaaaaatt ttactaaaac cagcgatctt cttattgcca atctggaaac ctccatggcg
    23461 gagggagaat ataccggtta tcctattttc aatactcctc ccgctttggc caaaaacctt
    23521 aaggacatag gggtggatat tgtaagcacc gccaacaacc actgtcttga taaaggtttc
    23581 aaaggacttg tctccaccat agagaatgta aaagcggcgg gacttttgca ggtgggaaca
    23641 tatgcagcac agcaagatcg tgacgaatcc catggtgttt tagttaaaga ggtcaacgga
    23701 ataagtatgg cgatagttaa ttattcctac ggtaccaatg ttataaggat accggcggga
    23761 aaggagtttt gcgttaatct tctgtatgaa gactatttag gtccctattc aatattaaat
    23821 aaggaacaga tttcttccga cttggaatat gcgcgctccc ttgataccga cctcataata
    23881 gcccttattc actggggcac cgaatatgtt acaacggaaa atgaaaagca acgacaagcc
    23941 gcacaatttc tgtttgataa cggcgtggat atattgatag gaaaccaccc ccatgtgctg
    24001 caacctatgg gaatacagac gatacgcacc ccggaaggtc aagaaaagca ggtattcgta
    24061 tgttattcgt tgggtaactt catgtccaat ataaccagaa ctcattacac actgacttcg
    24121 gcaatattga atctctatat agaaaaaaat cttgaaaccg atgtaaccgt aattaaagga
    24181 tatgattatg tgcccacata tatgcttact ccggaatggg gaggctccag accatatgtg
    24241 cttcccgttc atgatgccat aaaagaatat gaaaacggtt caagtacatt gataaatgaa
    24301 aaaatttaca aggatttact ggcatctttg aagatttgtc atgatatttt aggtcgcgat
    24361 tacgattatt actatataaa aagaacagat taatctattg tttatcttta agtttcgctc
    24421 tcatacgcat cacgggatcc aatatctttt ttctaatgcg catacttaag ggggttattt
    24481 ccagcaattc atcttctttt atatattcca ttgcctgttc cagactcatt tttttcggag
    24541 gggacaaccg caaagcttca tcactacccg ccgctctcat attcgtaaca tgtttctttt
    24601 tgcatacatt gatcgtgatg tcctcatcct tgggagtctc gcccacaacc ataccggcat
    24661 atacttcctc tccgggattt ataaaaagca ctcctctttc ctgagcatta tataagccgt
    24721 atgtaaccgc tgttcccgtt tcaaaggcaa ttagcgaacc gttgcttctg tatcctatct
    24781 cgcctttgta tggagcatat ccttcataga gggtattcat tatgccttct cccgcggtat
    24841 cggtcagaaa ctcgttcctg tagccgaaca agcccctcgc cggaacagaa aactccattt
    24901 tcattcttgc accggacaga ttcatgcaca gcatctcgcc ttttcttctt cccattttct
    24961 cacatacagc ccctacgcag tcggaaggta cgtcaatata tacatgttcc atgggttcct
    25021 gctttactcc gtctatatcc gtaaacagca ccctgggtgt actcaattga aattcatatc
    25081 cttctctcct catggtttcc accagaatgg acaggtgcat ttctcctctt cccataacaa
    25141 taaaagcatc ggcggattcc gtttccttca cttttaacga aacatccttc agtgtttctc
    25201 tgaacaaccg ctctcttaaa tgcctggatg ttacatattt accttctctt ccggcaaacg
    25261 gactgtcatt aaccgagaag gtcatttcta ttgtaggagg ggaaattttt gcaaattcta
    25321 ttgcttcgac aaattccgga tcacatatcg tgtctcctat agatatctca ggtattccgg
    25381 aaaaagaaat aatatctcca acatatgctt cttcaacagg ctctctttta actccttcaa
    25441 atgaatatat attggttacc tttgccctgt gaggttcttc gggattatgg aagttaacta
    25501 cggcaacatc gctgtttgcc ttaatgtgac ctctttctat tctgcctatg gctattcttc
    25561 cgacatagtc attgtagtca atggaagaaa taagcacctg cagcggccta ttttcgtcgc
    25621 cttccggagg attgatatgt tgcaaaacgg tatcaaacag aggcgacaaa tccgttcctt
    25681 ttatttccgg gttcagtgaa gcagctcctt cttttgcgga acagtacaca taaggactgt
    25741 ccaattgcgt gtcgtttgcc tgcaattcca taagaagttc cagcacgtcg ttttccgtct
    25801 ttcggatatc cgcaccttcc ctgtccactt tatttaccac aattatgact ttatgattaa
    25861 gttccagagc tttggaaaga acaaaccttg tctgcggcat aggcccttca aaggcatcca
    25921 ccaataacag tactccgtca accattttaa gtacgcgttc cacttctccg gaaaaatccg
    25981 catgtccggg ggtatccact atattaatct tggtattctt gtatcttacc gaagcattct
    26041 tggcaagaat agttataccc ttctctctct ccaaatcacc ggaatccatt actctgtccg
    26101 caacaacctg atttttcctg aacactcccg cctgtttaag catggcatcc accagtgtgg
    26161 tcttgccgtg gtctacatgc gctattattg ctatatttct taagtcttcc cttatcataa
    26221 gctttcctca aaagttttca acttattata cacctgtaag attaaaagaa aagcataata
    26281 ttttatactt aacatcaaag gctatcccct ttaacttttt tgagaaacta ttctgttttc
    26341 atatgatatg tctattcttt atattccgta ttttttatat cggtaataac actttccaaa
    26401 tctgcggttt ttaaaatagt agtcgtatat ccagctctaa cctcattcaa ggtgttatct
    26461 ttaattataa actgaccgtt atgtaaaccg tttataatgt aaacattctc agcgacaggc
    26521 cccttatatt ccctcagaaa taataacact tcggtatttt taataagata aggattctct
    26581 ttcatttttg tttgcagtat ttctaatgtc aggtttccta aatctcctct taaacagtct
    26641 ttaatctcga cctcggtaac ggcaaagatt atgtcatcat acacaaactc tttgttatcc
    26701 gaaacaactg tcgctattac ggcaacgggt gatttttcca acaagtcttc ataggtgctg
    26761 aatgtgtcaa ctgatttccc tcttccctgt gtaatatttg cccttataac aaaaaacaaa
    26821 agaacggcaa ccgataatat taagacgatt accgctattg tccatctgct ttttttctta
    26881 gttacggtag gcactttatt tcctccatga taataatcgg actctcctct gtccggaatt
    26941 attccatcat gtattatctg acgaatagtt tcaacctaaa gttctcaggt cattaaataa
    27001 aggaacatta catacataaa aatgatacaa tatccgtata tcattgtatt aaggagtgtg
    27061 ccatgaaaaa atttgcattt ataggcggcg gcagtttcgg ctttacacgt aatctggtga
    27121 aggaccttct cactttcccc gcatttcgtg atgcccatat cgttctgatg gatatagatc
    27181 ccgaaagatt ggattatata catcaggctg tcaggaagat tataaaagcg ggaaaatatc
    27241 ccgcaaaagt taccgcaact acagacaggg ttaaagctct taaaggagcg gacggtgtac
    27301 tgtgtacgat actttccggc gatgtggatg tttggcagca tgatatcctc attcccaaaa
    27361 aatacggggt ggatataaat atcggagaca ccagaggtcc cgcaggcatt ttccgttttt
    27421 taagaacggt accggtaatg ctggatatat gtaaagacat tgaaaaatac tgtcccgatg
    27481 cggttttctt aaactacaca aacccgatgg ctatgttatg tatggccatg cagaagaaat
    27541 tccccaatct taagataacc ggcttgtgtc acagtgttca gggaacggcg ggaatgcttg
    27601 caaaatggat aggtgctccc atgaatgaaa taacttacaa atgtgcgggc gtaaaccatc
    27661 aggctttcta tttagaatac aagtggaacg gaaaagacgc gtatccttta atcagggaag
    27721 ccatcaaaga tcctgaaatt taccaaaaag aacttgtccg gaacaatatg ttcgagcatc
    27781 tggattatta tgtaacggaa tcttccggcc acaacagcga atatgtcgca tggtacagaa
    27841 agaatcccga attattggaa aaatactgta tgaacggtac aaactggaac cccggacatt
    27901 atgcatatat tcttaatgaa tatctgaaac ggaaagacac ctggcgtgac tccattaagg
    27961 aatggcttgc aaaggaggat attaatctgg agcgcggtta tgagtatgct acatatattt
    28021 tcaatgcggt attcggagat aacactatgt tcaaattcaa cggaaatgta atgaatcacg
    28081 gaattataga gaatcttccg tatgactgct gtgttgaagt tcctattctt gcatcaagga
    28141 attcattgga accgatacat ataggagcta tgcccgatca gcttgccacc cttataaaca
    28201 caacggcccg ctgtgaggta atggctgtgg atgcggcact ggaacgtaac ccgagaaagg
    28261 tattccatgc ggttgcattc gatccgctta cttccgccgt actgtcgctt gatcagataa
    28321 aagagatggt tgatgaaatg atggagaaga ataaagagta cctcggttat ttgtataaat
    28381 aataataaat aaggagtttt ctctttatcc tgttatccaa gacaaaaggt tatgccgttg
    28441 cataaccttt tttgtgtatg acgggcatgt tgtcagtctg tggtgaaagt ccacaagggg
    28501 cgtagttg
//
LOCUS       DAOSOM010000027        25651 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_1919_length_25651_cov_8.763010, whole genome shotgun sequence.
ACCESSION   DAOSOM010000027 DAOSOM010000000
VERSION     DAOSOM010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 25651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25651
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1919_length_25651_cov_8.763010"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..943
                     /locus_tag="PLF14_07030"
     CDS             164..943
                     /locus_tag="PLF14_07030"
                     /inference="COORDINATES: protein motif:HMM:NF024231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOT71079.1"
                     /translation="MKTRKIAIAGIMGALCIVMALTPIGYIPVGPTKATIIHIPVIVA
                     AILEGPLVGAIVGLIFGLSSVLNALLFPTVTSYVFLNPLVSVVPRILVGLVAYFVYAL
                     FKKLKKPLSVTLLTIMLFATAYYLLNQVVTNFQEGKTFYAIFNIFLLLVVFGAEIYAI
                     LNYESKNFAVALSAAVATLTNTVLVLSMIGLIYGGEWVAKLGVEPSMAKDFLVGIGVA
                     NGIPETVGAVIIGSIVVEALTKLVYRKDQPVEISAAEAKAE"
     gene            1393..2661
                     /gene="murA"
                     /locus_tag="PLF14_07035"
     CDS             1393..2661
                     /gene="murA"
                     /locus_tag="PLF14_07035"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOT71080.1"
                     /translation="MSTEDRIVVKGGKKLEGDVTVGGSKNAAVAIICASAMVKGKVFL
                     ENVPRITDIQVLLETLKQIGASVQWTGDKTVEVDATGVNSPICDNPDLSTLRGSYYFV
                     GSLLGRFRQAKVVFPGGCNFAPRPIDQHVYAFETLGASVETTGGYITAKTKKLVGNSI
                     YFDNYSVGATINSILTATLAEGTTTIENANREPHVVDVANFLNMMGADIKGAGTNAIR
                     IKGVKELTGGRSYSICPDMIEAGTLMIAAAATKGTVTLHNVMPEHMDALSMKLTEMGV
                     TVEAMDDSITVKANVKRVKNTNIKTMPYPGFPTDLHPQFATLLSVADGESRITEGVFP
                     TRFQYVEQLQKLGANIQLSGESAIIKGVPKLIGAPVLPTDLRAAAAMVIAGLMAEGQT
                     TIYNLEYLDRGYEDFIKKLQSLKAVIERGK"
     gene            2663..3718
                     /locus_tag="PLF14_07040"
     CDS             2663..3718
                     /locus_tag="PLF14_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008371255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOT71081.1"
                     /translation="MKYVELIRSLTDANGISGFEDEVVDVIKKYAVSEKVAVDTMNNV
                     FLGKKQNAGNRPLVMIDAHSDEVGFMVQFITGNGLLKFIPIGGWQDANVAAQRVRVRN
                     RNGVYIKGVVASIPPHFLTAAQKDAPPKIENMLIDVGATSREEVINDFGIDIAAPVVP
                     DVDFSMNSNGIMCAKAFDDRIGAAAVIATMEDLAREELNIDLIGTISTQEEVGLRGAE
                     VTVKTVNPDIAIVYEGTPADDTFTSGYETQAALKKGPQLRHRDGSMITNPRFLAFVRS
                     IAKQYNIPFQDAVRAGGGTDGGKIHLVTGAVPTVVIGIPVRYAHTHYGYCAYDDFINA
                     VELGKAVLRNIPGDLNY"
     gene            complement(3904..5886)
                     /locus_tag="PLF14_07045"
     CDS             complement(3904..5886)
                     /locus_tag="PLF14_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017212430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOT71082.1"
                     /translation="MKEEIKRPVFPKRAVITSGMPYGNKGLHFGHIGGLFVHADVFAR
                     FMRDRIGEENVIFISGTDCYGSPILESYRKLKESGNTEHKNIVEYVESNHIKQKNTLD
                     AYEISLNHFGASGLYRDKEIHRKVSIEVFESLYKGGYLSVMSTPQFYDPKFKTLLNGR
                     QVEGKCPFEGCGSEKAYADECGLGHQYMPEELIDPKSTLSGLKPEIVDVENWYFRLED
                     FRDKMQEYTKDLREHSVTRKFVLDAIDEFLKPPVIYVQRKQIDDLEELEKAFPEHEKI
                     DEENKASITFVFKNLDDRDIARDVLNERQIRYRTGKTLVPFRLSGNIDWGVPIPEKDN
                     LKDLTFWVWPESLWAPISFLRTYLEEKGAKEDEWKKWWLDDESNVYQFIGEDNIYFYC
                     IAEMAMFAAMRHPDFDTLDMTKLHLPDIIANKHILFMDKKASSSSQIKPPMANDLLEY
                     YTAEQLRMHFISLGLTSKSSSFKPQALLPKEEREGVDPALKEGNLLTNVFNRLIRSCL
                     YSIQKYNDSIVTIGKVSEEIKQQSDDAILEYERHMYNNDIHRIAYVLDSYVRIINKHW
                     ANNIRVADANNDKTLRNQILIDCFHGIKTALLLLHPIAPTGCEKARQYIGFSEKLWSW
                     DYAFSSVEELLIDKDAKTIFLEPREDFFRKHDSQFE"
     misc_binding    complement(5931..6143)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6332..7663
                     /locus_tag="PLF14_07050"
     CDS             6332..7663
                     /locus_tag="PLF14_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006427541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT71083.1"
                     /translation="MLRRKVENDLLKWKRANNKTALLVTGARQVGKTASIRQFGKTYY
                     DNFIEINFITDINADNIFSGSLRADDIILNLSAFVKKALVPGKTLIFLDEIQECPNAR
                     TAIKFLVEDGRFDYIESGSLLGINYKEIPSLPVGYEEILQMYPMDFEEFLWANNVQED
                     TIDYLKDCFINEKCISEVVHEEIKKLFQRYMIIGGMPAVVSKFAETKDIGQVITVQKR
                     IMELYRQDITKYSETEKMRIRQIFDHIPAELNKNNKRFTLSDMDKNARYNRYESGFMW
                     LSDAGVSLPCYNISQPVLPFELNTKYNLFKLFLCDTGLLCSQSLGNIQFAILNGDTSV
                     NMGSILENTIAQLLVSNNFKLYYYNSKKVGEIDFIIQKGAIALPVECKSGKDYNLHKA
                     LDNLLSVKEYDMKEAIVLYSGNIKRENKILYLPWYMVSFITQPSIDSYIVN"
     gene            complement(7660..8754)
                     /locus_tag="PLF14_07055"
     CDS             complement(7660..8754)
                     /locus_tag="PLF14_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71084.1"
                     /translation="MDFYPVDYLKENISDSGCIRECIEQAVKINGHKRIIFNKRNWHI
                     DEAILLHSDMEIIVDGCFIKQNDGVFDNIFRGVNLKVDEKDPYGKPLYVEKMKNVSIK
                     GYNNAKLIGCDVNKKGFHPVKKEYEDMVGDNWGWRTFMICLSECEGIEISGLSIENTK
                     CWALSLDLCSYGDIHDIFIQSHVKNGDGIDLRAGCRHFRIWNITGNTSDDTVACTALL
                     HSKRRFPFKNYLYPLAPAVSVFDGDIRKLDIHDISVSDIRTGGLHHAVICLAADGLRV
                     YNIDIKNIEDIGEGNRSATVMLYTGYGENYADNDIHDINIYGVHSRFSKYAVHINTKV
                     HNVTVSDILQENKEGSLYEFADTEGITVLN"
     gene            complement(8949..9908)
                     /locus_tag="PLF14_07060"
     CDS             complement(8949..9908)
                     /locus_tag="PLF14_07060"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOT71085.1"
                     /translation="MIQTNYINSPIVITPFKKRVITYAFHDIDGTHSLIRDWPPVMSI
                     VLNDVIENGLKEGYDSDRNVKRLVENAGTKPLPETDRFCVESAGLSALTQMEWAIRRA
                     VQEKTVTVQCDDSVNSEIISLIWQGKEEWHHIAETEEMKQMLSENTPRLFKYYEKVLN
                     GFCRDKNLVLARKNPEKFRIKGSFRFLNYLKENGIKNYFVTGAVVQRGMGMFEEVETL
                     GYKIGHGEIVEDIIGSTWTDKVPKNVIMNQLLDKYGIKGENTMVVGDGRSEIQAGVDM
                     GAFTMSRLCAHMQYQKQLHIRMGTNVIVEDFEHEDIYKILIAK"
     gene            complement(9910..10941)
                     /locus_tag="PLF14_07065"
     CDS             complement(9910..10941)
                     /locus_tag="PLF14_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009595428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HOT71086.1"
                     /translation="MFDEATRKILHACPREALDESLFSTSPIITRYPDTILSRHDVPY
                     SAALTFNAGVVKTDDRYIMIFRNDYGSFEEEKIIGTNLGLAFSDDGIKWKVEPEPCFA
                     MADDEVLRVYDPRITVLEDRYIICFAVDTKHGVRGGIAETKDLKHFEVLSMSVPENRN
                     MVLFPEKINGNYVRLERPMPVYGRDGMKFDIFLSESPDLVYWGKSRLVMGVEHVPFAN
                     EKIGPGAPPIKTDKGWIMLFHAVDFDPARGKNGWEPTWKKRYTIGVALLDLKDPSKVI
                     GMYKRPLMIPEGYYELEQGYRTNALFPCAALVDEGNVVRIYYSAGDAIIRMATCDLDE
                     LIGLCTEPL"
     gene            complement(11124..14630)
                     /gene="nifJ"
                     /locus_tag="PLF14_07070"
     CDS             complement(11124..14630)
                     /gene="nifJ"
                     /locus_tag="PLF14_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOT71087.1"
                     /translation="MAKKLKTMDGNQAASYVSYAFTDVAAIYPITPSSPMAENVDEWA
                     AHGQKNMFGQEVKVVELQSEAGASGTVHGSLQAGALTTTYTASQGLLLMIPNMYKIAG
                     ELLPAVFHVSARAVAGHALSIFGDHSDIYATRQTGFALLASGGVQEVMDLAGVAHLAA
                     IKGRVPFVHFFDGFRTSHEIQKIEVIDYADFEELVDKDAIKAFRQRALNPSKPVLRGT
                     AQNPDIFFQAKESVNKFYDAIPAIVEDYMKKISKITGRTYNLFDYYGAKDADRVIVAM
                     GSVTDAAEEVVDYLTEKGEKVGLLKVRLYRPFSIKHFLNALPKTVKKIAVLDRTKEPG
                     ATGEPLYLDVVAAYKGDKNAPVIVGGRYGLGSKDTDPSQIAAVFANLSLKEPKNNFTI
                     GINDDVTYLSLPIKERINTTPKSTVSCKFWGLGSDGTVGANKNAIKIIGDHTDMYAQA
                     YFAYDSKKSGGITVSHLRFGKNPIKSTYLVSDADFVACHNRSYVDKYDIVSDVKKGGT
                     FLLNTGWTVDEMEEALPGHMKRYIANNNIRFYTIDAVKIAQELGLGGRTNMILMSAFF
                     KITNIIPIEDAVKYMKDAVLKSYGRKGEKVVNMNNAAVDRGIDNIVEVKVPASWKHAP
                     DLPKEDKKYPAFISDVVEVMNAQQGDSLPVSTFVGIEDGTWEQGTARYEKRGIAVNVP
                     EWLIDNCIQCNQCSYVCPHAAIRPFLVSEEEMKKAPKGFETKKAIGKGLDGITFRIQV
                     SPYDCTGCGNCVQVCPSKEKALVMKPIETQMDQAPNWTYATSLSKKETPFNKYSVKGS
                     QFNMPYLEFSGACPGCGETPYMKLITQLFGDRMMIANATGCSSIWGGSAPSTPYCKDE
                     FGYGPAWANSLFEDNAEYGLGMHIAVKHIREKIEELLKELLTTDANDSVKKAAQDWLD
                     NKNNGEKTREVSDALLKQLKACKSNELVKELLDRKDFFVKPSQWISGGDGWAYDIGYG
                     GLDHVLASGEDINVIVYDTEVYSNTGGQASKATPTAAVAKFAASGKKVKKKDLGMMAM
                     SYGYVYVAQVAMGADQNQFIKAVKEAEEYDGPSLIIAYAPCINHGIRTGMGTTQLQAK
                     KAVEAGYWHLYRYNPKLAEEGKNPFILDSKEPTGSFKDFLATETRYTSLKVTFPDHAE
                     ELFEKTEQDAKERYETYRKKAE"
     gene            14808..15638
                     /locus_tag="PLF14_07075"
     CDS             14808..15638
                     /locus_tag="PLF14_07075"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOT71088.1"
                     /translation="MKKSKYRWIYIVASIIVALVTVFLIFSAERQHNFEAMDPNGSDA
                     KWLINQPVAHRGYYNETTAPENSLEAFSEAIKKGYAIELDVRLTKDNHVIVFHDKNLK
                     RMTGFDSDVNMTTLEQIKTLTLLETSATIPELVEALEFINGSVPILLEIKSSGLSHNL
                     DKLVYSVIKEYEGSIAVQSFNPLSIRWFAKNAPSIARGILLRGNLKYAIANRFKDNLH
                     MLFSKPNFVAYKYENLVKTDFSQLRKDGLIVLGWTVGENMLNTREFTAHCDNIILEAE
                     "
     gene            15635..16060
                     /locus_tag="PLF14_07080"
     CDS             15635..16060
                     /locus_tag="PLF14_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71089.1"
                     /translation="MMLKGRTLLGYDDKPDKRKNLKYTDWLIIIIALSPIWATLLIAV
                     VVAICALFAALVLGFTLTGFGAVTLFVYSAFSLADDLWSSVMKMGICLFASGAVIIAV
                     AGSFKGIKVFFVLLKSGYMKIKNMLLLKKLEGQKNEQNP"
     gene            16044..16967
                     /locus_tag="PLF14_07085"
     CDS             16044..16967
                     /locus_tag="PLF14_07085"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOT71090.1"
                     /translation="MSRTLKAIIIGGVFMLLGLVIFLVGYFSNNRKADSLLEFEIRET
                     TPLSLKSYDAPQPDSITMISIITKRMPVTIQSHEEQYIRLEYYENPEQSFEITQAGGK
                     LIVKKNDLGFFKENILLNLKSLLVKEKQDNGITVYMPSSYTGELLVSTTFGDISVRNA
                     LAFSKIKKIDFKNYRADIKMNSLSGNILNLMNTGGIIECSDISFEDITVISANADVKL
                     NYISSDYTDFLINKSSLQAAGIISDNIKIETVSSQIDMAINAYSVQYSVVIEKSEKSQ
                     INLTENIVENADKRIRIKADDSIISVSFSLD"
     gene            17039..17866
                     /locus_tag="PLF14_07090"
     CDS             17039..17866
                     /locus_tag="PLF14_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019879387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOT71091.1"
                     /translation="MYRILERKDLNSDVVYMKVDAPAVAKKCEPGQFIILRVDETGER
                     IPLTISDYNREEGWVSIIFQKVGKTTYLLDTLQQGDYILDFVGPLGIASHTDNMSRVC
                     VVGGGLGCAIAYPQAKKLFGMGLHVDVIAGFRNKSLVFLQEEMKRNSTNYYLMTDDGT
                     AGEKGMVTKKLKELIEGGANYDTVIAIGPVVMMKYVSLLTKEYNIKTIVSMNPIMIDG
                     TGMCGGCRLTVDGQTKFACVDGPDFDGHKVDFDGLMQRLSMYKEQEKHKCNLFRGIE"
     gene            17866..19266
                     /gene="gltA"
                     /locus_tag="PLF14_07095"
     CDS             17866..19266
                     /gene="gltA"
                     /locus_tag="PLF14_07095"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOT71092.1"
                     /translation="MAKINMTPEKTKMPEQDPDIRNRNFLEVATGYTPQMAQEEAQRC
                     LNCKHKPCVSGCPVNVNIPSFIQAVAQGDIEKAYEIITKTNSLPAICGRVCPQETQCE
                     LPCVRGIKGEPVAIGRLERYVADYMLAKETFIADTPKPTVKKNKKVAVIGSGPAGLTA
                     ASDLCDMGYDVTIFEALHKAGGVLVYGIPEFRLPESIVEKEIERLKAKGVCIELNMVI
                     GKIISIQELHEEEGFEAIFVGTGAGLPNFMNIPGENLNGVFSANEFLTRVNLMKAYDP
                     SYDTPIYFGSHVAVVGGGNVAMDAARAAKRLGAEQVYIVYRRSEQEMPARAEEVHHAK
                     EEGIEFRLLTNPTKILGENGWVTGMECVRMQLSEPGPDGRRRPVVIPGSEYVLKTTSV
                     IMAIGTSPNPLIKSTTEGLETNKWGCIVADEETGQTSIPYIFAGGDAVTGAATVILAM
                     GAGKKAAAAIDKYLDK"
     gene            19304..20458
                     /locus_tag="PLF14_07100"
     CDS             19304..20458
                     /locus_tag="PLF14_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOT71093.1"
                     /translation="MIKPFETVLPVGKLSIIGMRGCEEITKKVDWYLKQRAGYKDSDH
                     TFITEAICPRFGTGEAKGVILESIRGHDLFIICDVFNYGVTYEMYGMQVPMSPDDHYA
                     DLKRIIAATNGKARRITVIMPMLYEGRQHKRKYRESLDSAISLQELVGMGVENIITFD
                     AHDPRIQNAIPLNGFESVPVAYQMMKKLTASFPDIIFDKEHTLVISPDEGGMNRAIPF
                     ATMIGLNLGMFYKRRDFSVVEQGRNPIISHEFLGDSVEGKDVIVVEDIISSGESALDI
                     FYQLTKMKAKRIFMCCTFGLFCNGLEAFDKAYEEGAVTGVLTTNLVYRSPELLKRPWF
                     KEVDMSKYMSFIIDTLHHDASMSNLLNPVDRIDKLMEKVKSRQLEEGLIK"
     gene            complement(20469..21797)
                     /locus_tag="PLF14_07105"
     CDS             complement(20469..21797)
                     /locus_tag="PLF14_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT71094.1"
                     /translation="MFKRKIYDKLLEWKETSKGRTALLIEGARRVGKSTIVTEFGKNE
                     YKSYILIDFAFAPTGVKELFNDISDLDYLFLQLQLQFKEDLHERNSLIIFDEVQFCPK
                     ARQAVKKLVEDGRYDYIETGSLISIKKNVEHILIPSEERKIEMFPMDFEEFLWAIGDN
                     TTNNILRKFYNEKISPGQDQNRALMRKFRLYMLVGGMPQAVKAYIETNNFKFVDEVKR
                     DIIQLYEEDFYKIDSSGKISMLFDSIPSELSRHSSRYQVSSVLKNQRAATISEEIAEL
                     VASKTVLISYHSNDPSVGMTTNLSMEHFRLFMADTGLMVTCMFKDKDFTDNIIYEKLL
                     NNKLSKNLGVLYENIVAQTLAFNGNKLFYYTFYDETQKRSFEVDFLLSKDNKVSPIEV
                     KSSNYRTHRSMDEFANKYSNRINTKYVIHTQDLKKNGNLIYLPFYLAQFI"
     gene            complement(21934..23172)
                     /locus_tag="PLF14_07110"
     CDS             complement(21934..23172)
                     /locus_tag="PLF14_07110"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOT71095.1"
                     /translation="MNTINYRKRLIESQIERKMSYSGGVIIEGAKWAGKSTTAGIFSN
                     TVIKLHDPLIKKQYQILATISKDEVLKGEKPILFDEWQEVPEIWDFIRLDIDENNHKG
                     AYLLTGSTKKQNITTSHTGTGRINSVYMRPMSLYETGESNGLISLKELFSQPPLVPVK
                     SNITIDNIAHCICRGGWPGSLDLNKDLQLEIPKDLLESIIKRDIDEVDGITKDKEKFR
                     KIIKSYARNISTLAANSAIYEVIVNPKTFDTYMNSLKRLYIVEDVLPWSQNIRSSTRL
                     RKGTKRQFVDPSIAVAALGLSPDKLKNDFETFGFLFESIATRDIRVYAEEIGGTVYYY
                     NDSTGLEVDLIVELNDGRWGGIEVKLGENEADKASANLIKLANISATKPSFLMLLTNT
                     QMAYQRDDGVYVIPLGCLRP"
     gene            complement(23453..24631)
                     /locus_tag="PLF14_07115"
     CDS             complement(23453..24631)
                     /locus_tag="PLF14_07115"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016279976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HOT71096.1"
                     /translation="MAKPNVNYGNLKQSYLFRDIATKVAAYKKAHPDRKVISLGIGDV
                     TLPLAKKVVEALKAASEEMGKAETFRGYAPDQGYDFLREKISEHYKKRNVNVDISEIF
                     VSDGAKSDLGNITEIFSRDNIVMIPDPVYPVYMDTNVMDGRKIIFADANKENHFLAMP
                     DKRHADMIYLCSPNNPCGSVYSKAQLEKWVEYALKHNSVILFDAAYEAFITDDKLPRS
                     IFEIEGAEKCAIEFCSFSKLAGFTGTRCAYTVIKKELILEGFSLRDMWVRRQSTKLNG
                     VSYIIQRGAEAVFTEEGMKQCKDNIRYYLDNAKLISALLTEKGIYHTGGNNSPYIWMS
                     CPNNLKSWEFFDLLLNETCVVGTPGSGFGHNGEGYFRLTSFGSRENTIEAVERLSKVL
                     "
     gene            complement(24652..25533)
                     /gene="dapF"
                     /locus_tag="PLF14_07120"
     CDS             complement(24652..25533)
                     /gene="dapF"
                     /locus_tag="PLF14_07120"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019879749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOT71097.1"
                     /translation="MKFTKMHGAGNDYIYVNCMECKIDNPSSLAIKLSDRHKGIGSDG
                     LVLIQPSKVAHFYMRMFNSDGSEGAMCGNAIRCVAKYVYDNRLTDKLKLNIETKSGIK
                     HLKLFPDKKGKIDSVEVDMGIAYLKRKEIPMVYDNSNRDETYINKPLVVDGKKYNVTC
                     VSMGNPHCVIFMNDKDNKSNLMQSLDRLDLDNIGPDFERHSYFPDRINTEFVEIIDRS
                     TLKMRVYERGSGETMACGTGACATVVAAVLNGICERHSEVSVILRGGTLKITYLYDGR
                     VIMKGEAVHVFDTEISI"
BASE COUNT         7307 a         5457 c         5007 g         7880 t
ORIGIN      
        1 cccatgactt ggagatatcc gctgatgcgg acgaagtttt aagtatgaga gacgggaaac
       61 tgattgataa atagtcatat tttcattgca catctgtctt ttctgttata taattcacgc
      121 caagataggt atcccttaag ggaactgaag cggaggtagt aatatgaaga cacgtaaaat
      181 cgcgatagcg ggcataatgg gtgcattatg catagtaatg gctttaacgc cgataggtta
      241 tattcccgtc ggaccgacaa aagcaacaat catacacatt cctgtaattg tagcggcaat
      301 tttagaagga cctttggtag gagcgatagt aggcttgatt ttcgggttgt ccagtgtttt
      361 gaatgccctg ttattcccta ctgtaacatc atatgtattt ttgaatccgt tagtatcggt
      421 agttccgaga atacttgtcg gccttgtggc atactttgta tatgctttgt tcaaaaaact
      481 gaaaaagcct ctttccgtta cattacttac tattatgctc tttgcaacgg catattatct
      541 tcttaatcag gttgtaacca attttcagga aggcaaaact ttttatgcca tattcaatat
      601 attcctttta ctggtggttt tcggtgccga gatttatgcc atactgaact acgaatccaa
      661 gaactttgcc gttgctctgt ccgcagcggt tgcgacattg acgaatactg ttctggtact
      721 tagtatgatc ggattgattt acggcggaga atgggttgcc aaattaggcg ttgaaccgtc
      781 catggcaaaa gattttctgg tagggatagg agtggcaaac ggaataccgg aaacggtagg
      841 cgccgttata atcggatcca ttgttgtgga agcactgaca aaactggtat acaggaaaga
      901 ccaacctgtt gaaatatcgg cagctgaagc aaaggccgaa tagttactgc ccggttccca
      961 tacgatgaat cgtatgttaa ctatatagac aggccggata ttttatccgg cttgtcgctt
     1021 ttattgttac aaagatgaag agaccgcttt cgcaatctct tcagggggaa atttgatctt
     1081 tatggggtag tggttaatga ttacatgata catatgtatt atgaactcaa tgttaatatt
     1141 atgtggaacg agcatgaact taacattttt gaataatttt ttcttaaaaa gaaaaaagac
     1201 tgcatggggt agataacagt ctctcttcta aaagggtgga acctttttct aatttgtggt
     1261 aattctatgt taaatcttca atgtgatgaa tcggtgaaaa tgttatgaac acaatgaaaa
     1321 ttataaaatc attgaatatt cattcaataa aatgtatcat attaatattg agagacgatt
     1381 ggagatactt gattgagtac agaagataga attgttgtta aaggcggcaa aaagctggaa
     1441 ggcgatgtca ctgtaggtgg ttcaaaaaac gcggctgtag caataatatg cgcatcggcc
     1501 atggtcaaag gtaaagtttt tttggaaaat gttccgagga taaccgacat ccaggttttg
     1561 ctggaaaccc tcaaacagat aggtgcatcc gtgcaatgga caggagataa aaccgttgag
     1621 gttgatgcaa cgggtgtcaa ttcgcctata tgcgataatc ccgatttaag cacattgaga
     1681 ggctcctact attttgtagg atcacttttg ggaagattca gacaggcaaa agttgttttc
     1741 ccgggcggat gtaattttgc tccgagacct atcgatcagc atgtttatgc atttgagact
     1801 ttaggtgcat ctgtggaaac caccggcgga tatatcaccg caaaaacaaa aaaactcgta
     1861 ggtaacagta tttatttcga caactattcc gttggagcta ccattaactc catactcacg
     1921 gcgactctgg ccgaaggtac gacgacaata gaaaatgcca acagagaacc tcatgtagtg
     1981 gatgttgcca actttttgaa tatgatgggt gccgatatca aaggtgcggg tacaaatgcg
     2041 ataagaatta aaggtgttaa ggaacttacg ggaggcagat cctattccat atgtccggat
     2101 atgattgaag ctggtacgtt aatgatagcc gctgccgcaa caaaaggtac cgttacttta
     2161 cataacgtaa tgccggagca tatggatgct ctgtccatga aactgaccga gatgggggtc
     2221 accgttgaag caatggatga ttccataacg gtaaaagcca atgtaaagag agtaaaaaat
     2281 acaaatataa aaactatgcc ctatcccggt tttccgaccg atttacatcc tcagttcgca
     2341 acactgctgt ccgttgcgga cggagaaagc agaataacag aaggagtatt tcccacccgt
     2401 ttccagtatg tggagcaact tcagaaactc ggtgctaata tacagttaag cggcgaatcg
     2461 gcaatcataa aaggggtgcc taaattgata ggggctcccg tattgcctac cgaccttcgt
     2521 gccgcagcgg caatggttat agcggggctg atggcggagg gacagaccac tatatataat
     2581 cttgaatatc tcgacagagg atacgaagat ttcatcaaga agttgcagtc tctgaaagcc
     2641 gttatagaaa gaggtaagta aaatgaaata tgttgaactt ataagaagct tgaccgatgc
     2701 aaacggtata tcgggttttg aggatgaagt tgtcgatgta ataaagaaat atgcggtgtc
     2761 cgaaaaagtc gcggtggaca ccatgaacaa tgtctttctg ggaaagaagc agaatgccgg
     2821 aaacagacct cttgttatga ttgatgcaca ttccgatgaa gtaggcttca tggttcagtt
     2881 cataacggga aacgggttgt tgaagttcat tcccatagga ggctggcagg atgccaatgt
     2941 agcggcacaa agagtaaggg taagaaaccg caacggtgtt tatataaagg gtgtagtagc
     3001 atcgattcct cctcattttc tgaccgcagc gcagaaagat gccccgccta agattgaaaa
     3061 catgcttata gacgtaggtg caacatccag agaggaagtt ataaacgatt tcggtataga
     3121 tatagcggct ccggtagttc ccgatgtgga tttttccatg aacagtaatg gcattatgtg
     3181 tgcaaaagca tttgatgaca gaataggtgc ggcagctgta atcgcaacaa tggaagattt
     3241 agcccgtgaa gaactcaata tagaccttat aggaaccata tccacacagg aagaggtagg
     3301 cttaagagga gcggaagtaa ccgttaaaac ggttaatccc gatatagcca tagtatatga
     3361 aggcacaccg gcggatgata cctttacaag cggttatgaa acacaggcgg ctctcaaaaa
     3421 aggacctcag ctacgtcaca gggacggaag catgattacc aaccccagat ttcttgcctt
     3481 tgtcagaagc attgcaaaac aatacaacat cccgttccag gatgcggtaa gagcaggagg
     3541 cggaaccgac ggaggtaaaa ttcatctggt gacgggagcc gtacctacgg tggtaatagg
     3601 tattcccgtt aggtatgcac atacccacta cggttactgt gcatatgatg attttattaa
     3661 tgcggttgaa ctcggaaaag ccgtactgcg taatataccc ggcgatttga attattaaag
     3721 aagatgcctt gaacttcatg tttgcatttt cggaaaaata gatgtagggg aactgactat
     3781 ttagtttcat gaaggggcat ccgaggtgat attaacagaa tcaagcaaca tataccacag
     3841 acaaaacagt attatttaat acaatatctt tcttttcaga agttcatata agcaacttaa
     3901 aatctattca aactggctgt catgttttcg gaaaaagtct tctctcggct ccaagaatat
     3961 tgttttggca tctttgtcga taagaagctc ttctacggag gaaaacgcat aatcccagct
     4021 ccacagtttt tcggaaaacc ctatgtattg tcttgccttc tcgcaacctg tcggagcaat
     4081 gggatgtaaa agaagaagcg cggtctttat gccgtggaaa caatcgataa gtatctgatt
     4141 tctcaatgtc ttgtcattat tggcatcggc cactcttatg ttgttggccc agtgcttatt
     4201 tatgattctc acataactgt ccagtacata tgcaattctg tgaatgtcat tgttatacat
     4261 atgtctttca tattccagta ttgcatcatc cgattgctgc ttgatttctt ccgatacttt
     4321 tcctatggtt actatcgaat cgttgtattt ctgtatggaa tataaacagg atcttatcag
     4381 tctgttgaaa acattggtaa gaagattacc ctcttttaat gcggggtcca ctccctcccg
     4441 ctcttcttta ggaagtaaag cctgaggctt gaaggagctg ctcttacttg taagtcccaa
     4501 gcttataaaa tgcattctca actgctctgc ggtatagtat tccaataaat cattcgccat
     4561 cggcggcttt atctgtgaac tgctgctggc ctttttatcc ataaacagaa tatgtttatt
     4621 ggcaataata tctggaagat gaagcttggt catatccaat gtgtcaaaat ccggatgcct
     4681 cattgccgca aacatcgcca tctccgctat gcagtagaag tatatgttgt cttctcctat
     4741 gaactgatat acattgcttt catcatccag ccaccatttt ttccattcat cctcttttgc
     4801 tcccttttct tcaagatagg ttctcaaaaa tgatataggt gcccacaacg attccggcca
     4861 tacccagaaa gtaaggtctt tcagattatc tttttccgga atgggaactc cccagtctat
     4921 gtttcccgag agacgaaaag gaaccagagt cttgccggta cggtatcgga tctgcctttc
     4981 attcagtaca tcgcgcgcaa tatcgcggtc atcaagattc ttaaaaacaa aagtaatgga
     5041 agccttgttt tcctcatcta ttttttcgtg ctcggggaaa gccttttcca gctcttccaa
     5101 atcatctatc tgttttctct gaacatatat gacgggaggt ttcaaaaact catctattgc
     5161 gtcaagaaca aacttccttg taacggagtg ctctctcaaa tcttttgtat actcctgcat
     5221 cttatcccgg aaatcttcca gccggaaata ccagttctcc acatcaacta tttccggctt
     5281 taatcccgat aaagtgcttt tcggatctat cagttcctcc ggcatatact gatgtcccag
     5341 tccgcattcg tcggcatatg ctttttccga tccgcaccct tcaaacggac acttgccttc
     5401 cacctgtctg ccattcagta aagtcttgaa tttcggatcg taaaactgag gcgttgacat
     5461 aacagaaaga tacccgcctt tgtacagtga ttcaaaaact tcaattgata cctttctgtg
     5521 aatttcttta tctctgtata agcccgatgc tccgaaatga ttgaggctta tctcatatgc
     5581 atccagtgtg tttttttgct taatatgatt ggactctaca tattcaacta tatttttatg
     5641 ttcggtattg ccgctttctt taagttttct gtaactttcc agaatgggag agccgtaaca
     5701 gtcggttccc gatatgaaga tgacgttttc ctctcctatt ctgtcccgca tgaatctggc
     5761 aaagacatcc gcatgaacga ataatcctcc tatatgcccg aaatgcagtc ctttgtttcc
     5821 gtacggcata cctgatgtta taactgccct tttgggaaat accggtcttt taatttcttc
     5881 tttcataatt aactcctatc tcaaaaaagc ccccgtaaaa ctaatgtttt atcaggggcg
     5941 ataatatcgc ggtgccaccc ttcttcgcat atccgtcgcc gtatatgcct tataaagtac
     6001 aaaagtactt ccgtccttta acgggaaccc ccggtgttca cggctccaag gcctgtttta
     6061 tgtgatgtgt aatgccttat ctcagcatca aggctctctg taaatccatt gaccacacta
     6121 ctttttctta tcatcgcctt accaaaaaag tatagaacaa gaggagaatt atgtcaaata
     6181 ctctttcttg ggaaaaacgt tacaaaacaa ccttctttac atcggaaaaa cgttacaaaa
     6241 tgctccttaa tatatgagaa atacgttaca cacatattga cttatgcctt aaatacggat
     6301 aaaattcaaa taaagtgtta agaggtgtga aatgctgaga agaaaagtag aaaatgattt
     6361 actgaaatgg aaacgggcga ataataagac ggctcttctt gttaccggag caagacaggt
     6421 cggcaagacg gcatctataa gacaattcgg caaaacatat tatgataact tcatagagat
     6481 aaattttatt acggacataa atgccgacaa catattcagc ggcagcttaa gagctgatga
     6541 tattatttta aacctctcag cttttgtgaa aaaggctctt gtgcccggta aaacccttat
     6601 attccttgac gaaattcagg aatgtccaaa tgcaagaacg gcaattaagt tccttgtgga
     6661 agacgggaga tttgactata ttgaatccgg ctctctcctt ggaattaact ataaagaaat
     6721 accttcactt cccgtaggat atgaagaaat actgcaaatg tatcccatgg attttgagga
     6781 attcctttgg gccaacaatg ttcaggaaga cactatagac tacttaaaag actgttttat
     6841 taatgaaaag tgtataagtg aagtagttca tgaagagata aagaagttgt ttcaaagata
     6901 tatgataata ggcggcatgc ccgccgtggt ttctaaattt gcggaaacaa aagatatagg
     6961 acaggtcata actgttcaga aaagaataat ggaactgtat aggcaggata taaccaagta
     7021 ctccgaaaca gaaaagatga gaataagaca gatctttgat cacatacctg cggaattgaa
     7081 taaaaacaat aaaaggttta ctttatcaga catggataaa aatgccagat acaacagata
     7141 cgaaagcgga tttatgtggt tatccgacgc aggcgtttca cttccctgct ataacatatc
     7201 tcaaccggtg ttaccttttg aacttaatac caagtataat cttttcaaac tcttcctgtg
     7261 tgatacgggc ctattgtgct cacagtcttt gggtaatatc caatttgcca ttctaaacgg
     7321 cgatacttcg gttaatatgg gaagtatttt agaaaacacc atagcgcaat tactggtttc
     7381 aaacaacttc aaactctatt actacaacag taaaaaagta ggagaaattg attttataat
     7441 acaaaaaggc gctattgcct tacctgtcga atgtaaatcc ggtaaagact ataatctgca
     7501 taaagccttg gataatcttt tatctgtaaa ggaatacgat atgaaagaag caatagttct
     7561 gtattccggc aacattaaaa gagaaaataa aattctttat ctgccttggt atatggtttc
     7621 ttttataaca caaccttcaa tagactcgta tatagttaat tagtttaata ccgttatacc
     7681 ttccgtatcg gcaaactcgt aaagactgcc ttctttgttt tcctgtaaga tatcggatac
     7741 ggtgacattg tgcacttttg tgtttatatg caccgcatat ttggagaatc ttgaatgaac
     7801 tccgtatata tttatgtcat gaatatcgtt atccgcgtaa ttttctccgt acccggtata
     7861 caacataacc gttgccgatc tgttaccttc ccctatgtcc tctatattct ttatatctat
     7921 gttatatact ctcaacccgt cggctgccag acatatgacc gcgtggtgta agcctcccgt
     7981 gcggatatcg gaaacggaaa tatcatgtat gtccagcttg cgtatgtctc cgtcaaatac
     8041 ggaaaccgcc ggagctaaag gatacagata atttttaaac gggaaccgtc gcttgctatg
     8101 taaaagcgca gtgcatgcca ccgtatcatc cgatgtattg ccggttatat tccatatgcg
     8161 gaaatgacgg catcccgctc ttaaatcgat accgtctccg tttttaacat gcgattgaat
     8221 aaaaatatca tgaatatctc cgtaagaaca caaatccagc gacagcgccc agcatttagt
     8281 gttttcaata cttaaccccg atatctctat gccttcacat tcggataagc atatcataaa
     8341 ggtcctccac ccccagttat cccctaccat atcctcatac tctttcttaa cgggatgaaa
     8401 ccctttttta tttacatcac accctataag ctttgcgttg ttatatccct ttatggatac
     8461 atttttcatt ttttccacat acaacggttt accatacggg tctttctcat caacttttaa
     8521 attgactcct ctgaatatat tgtcaaacac accgtcattc tgctttataa aacacccgtc
     8581 aactatgatt tccatatcgc tgtgcagaag aatggcttca tctatatgcc agttgcgttt
     8641 gttaaatatt atcctcttat gaccgttgat tttaacagcc tgctctatgc attctcttat
     8701 gcacccggag tcgctaatat tttcttttaa gtaatcaaca ggataaaaat ccatacctct
     8761 ccttataaca taaaaatata acggtttaca aacggagtat atctgaaaag caggtatatg
     8821 acaaatgatt tctttctttt ttccgggaat aagagacccg gaaaaactaa aagaagagaa
     8881 aacgattgca taaaaaagga actgttaaaa atacctgaca gtcccctttt ttatgcgcta
     8941 ctcctctttt acttcgcaat taatatcttg tatatatctt catgttcaaa atcttccact
     9001 attacgttgg tacccatcct gatatgaagc tgcttctgat actgcatatg agcacataat
     9061 ctgctcattg taaaagcacc catatccact cctgcctgta tttccgagcg tccgtctccg
     9121 acaaccatgg tgttttcacc tttgataccg tacttatcca gcagttgatt cattatgacg
     9181 tttttgggaa ctttatccgt ccatgtggag cctattatat cttccacaat ctctccgtga
     9241 cctatcttat accccagcgt ctccacttcc tcaaacatac ccattcctct ttgcaccacc
     9301 gctccggtaa cgaaatagtt cttaattccg ttttctttaa gatagtttaa aaatctgaaa
     9361 gagcccttta ttcggaattt ctccgggttt tttcttgcaa gtacgaggtt tttatcccga
     9421 cagaaaccgt tcaatacttt ttcatagtat ttaaacaatc tgggggtgtt ctccgaaagc
     9481 atctgtttca tctcttcggt ttccgctata tgatgccatt cttcttttcc ctgccatata
     9541 aggctgatta tctcgctgtt taccgaatca tcacactgca ctgtgacggt tttctcctga
     9601 accgcccttc ttatcgccca ttccatctga gtcaaagcgg aaaggcctgc cgattccaca
     9661 cagaacctgt cggtctccgg taagggtttg gttccggcat tttccaccag tctttttaca
     9721 ttcctatcgc tgtcatagcc ttcttttaat ccgttttcta ttacatcgtt taaaactatg
     9781 ctcattacgg gtggccagtc tcgtattaat gaatgtgtgc cgtcaatatc atgaaaggca
     9841 taagtaatca cccttttttt gaaaggggtg attactatgg gtgaattaat ataatttgtc
     9901 tgtatcatat tataacggtt cggtacacaa gcctataagc tcatcaagat cgcacgttgc
     9961 catacggatt atggcatctc ccgcactgta atatatcctt accacattac cttcatcaac
    10021 aagagcggca cacgggaaca atgcattggt tctgtatccc tgttccagct cataatatcc
    10081 ttcaggtatc attaacgggc gtttatacat tcctattact tttgacggat ctttcaaatc
    10141 cagtaaagca acacctattg tatatctctt tttccatgta ggctcccatc cgtttttccc
    10201 tcttgcggga tcaaaatcca ctgcgtggaa caacattatc cagcctttgt ccgttttgat
    10261 aggaggtgca cccggtccta tcttctcatt tgcgaacggg acatgctcta cacccattac
    10321 cagcctggat ttaccccagt aaacaaggtc aggtgattcc gaaaggaata tatcgaattt
    10381 cattccgtct cttccgtaaa ccggcatggg tctttctaaa cgaacatagt taccgtttat
    10441 tttctcgggg aacagaacca tatttctgtt ttccggtacc gacatcgaca gtacttcaaa
    10501 atgtttgagg tcttttgttt cggctattcc tccgcgaact ccgtgcttgg tatccactgc
    10561 aaaacagatt atatatctgt cctccaagac ggttattctc gggtcataaa ctctgagcac
    10621 ttcatcgtca gccatggcaa aacacggttc cggctccacc ttccacttaa ttccgtcatc
    10681 actgaaagcc agtcccagat tggttccgat aatcttctct tcttcgaatg aaccgtaatc
    10741 attacggaaa atcatgatgt atctgtcatc ggtctttaca actcctgcat taaaggtcaa
    10801 tgccgcggaa taaggaacat catgtctgct cagaatcgta tcgggatatc ttgttattat
    10861 ggggcttgtg gaaaacagac tctcatcaag cgcttctctc ggacaagcat gtaatatctt
    10921 gcgggttgct tcatcaaaca tttgaaaata ccttcctttt tatatattgt tatagcaact
    10981 cttaatatat acaaatccgg ctgctaagtc aacatcaagc ggcgtgtttt ccggttattg
    11041 aagtatcggc aaactcccct tcacatacaa aatgactgca cattttgtac agtcatcttg
    11101 atttattcga atattacctt tacttattcg gctttctttc tgtaggtctc gtatctttct
    11161 ttggcatcct gttcggtctt ttcaaacaac tcttccgcat ggtcggggaa tgtgaccttc
    11221 agagaagtat atcttgtttc ggtagccagg aaatccttga agcttcccgt gggttccttg
    11281 gagtccagaa tgaacggatt ctttccttcc tcggcaagtt tcggattgta tctgtaaaga
    11341 tgccagtatc ccgcttccac ggctttcttt gcctgtagct gagtggttcc catgcctgtt
    11401 cttataccgt gattgataca aggagcatat gctattatga gcgaaggccc gtcatattct
    11461 tcggcttctt ttacggcctt gatgaattga ttctgatctg cacccatggc aacctgtgca
    11521 acatagacat atccgtagga cattgccatc attcccaagt ctttcttttt gacctttttg
    11581 cccgatgctg caaatttcgc aaccgccgct gtgggagtcg ctttacttgc ctgtccgccg
    11641 gtattggaat atacttcggt gtcgtaaact ataacattta tatcctcgcc tgaagcaaga
    11701 acatggtcca gtccgccgta acctatgtca taagcccatc cgtctcctcc ggaaatccac
    11761 tgtgaaggtt ttacgaagaa gtctttcctg tctaagagtt cttttactaa ttcattactc
    11821 ttgcaggctt tgagctgctt aagaagagca tcacttactt ctcttgtttt ctcaccgtta
    11881 ttcttattat ccaaccaatc ctgagctgcc ttcttaacgc tgtcgtttgc atcggttgtt
    11941 aaaagctctt tcaacagttc ctctattttc tcccttatat gtttaacggc tatatgcata
    12001 cccaatccgt actcggcatt atcttcaaac agtgaatttg cccaagcggg accatatccg
    12061 aattcatcct tacaataagg tgtagaaggt gcagaacctc cccagataga actgcatccc
    12121 gttgcatttg ctatcatcat cctgtctccg aagagttggg tgataagctt catgtaagga
    12181 gtctctccgc atcccggaca agctccggag aattccagat aaggcatgtt gaactggctg
    12241 cccttgacgc tgtatttatt gaacggtgtt tcttttttcg aaagacttgt ggcatatgtc
    12301 cagttgggag cctgatccat ctgcgtttca ataggcttca taaccagtgc cttctccttg
    12361 gaaggacata cctggacaca gtttccgcaa ccggtacagt catacggtga aacctgtatt
    12421 ctgaaagtaa ttccgtccag tccttttcct atagccttct ttgtttcaaa gcccttgggt
    12481 gccttcttca tttcctcttc acttacaagg aaaggtctga ttgccgcatg gggacataca
    12541 tacgagcatt gattacactg tatacaattg tctataagcc attcgggaac attaacggct
    12601 atgcctctct tttcatatct tgccgtaccc tgctcccatg ttccgtcttc tattcctaca
    12661 aatgtggata ccggcaggga atctccctgt tgagcattca tcacttccac tacatcggat
    12721 atgaatgcgg gatacttttt gtcttctttg ggaagatcgg gagcgtgttt ccatgatgcg
    12781 ggaactttta cttccactat attgtctatt ccgcggtcta cggcggcatt gttcatgtta
    12841 accacttttt caccttttct tccataagat ttaagtacgg catctttcat gtatttaacc
    12901 gcatcttcaa tgggaattat attggtaatc ttaaagaatg ccgacatcag tatcatgttg
    12961 gtccttccgc ccagtcccag ttcctgtgca attttcaccg catctattgt gtagaacctg
    13021 atgttgttgt tagcgatata tctcttcatg tgccccggaa gagcctcttc catttcatcc
    13081 accgtccatc cggtgttgag aaggaatgtt ccgcctttct tgacatcgga aacaatatcg
    13141 tatttatcca cataactgcg gttgtggcat gcaacaaaat cggcatcgga aactaaatat
    13201 gtcgacttga tgggattctt tccgaatcgc aagtgcgata cagtgattcc gccggatttt
    13261 ttggagtcgt acgcgaaata ggcttgcgca tacatatcgg tatggtcacc gattatcttg
    13321 atggcatttt tgtttgcccc taccgttccg tccgatccca gtccccagaa cttacagctt
    13381 accgtgctct tgggagtggt atttattctt tcctttatag gaagtgaaag atatgtaacg
    13441 tcatcattga ttcctatggt gaaattattc ttgggttctt tcagggacag atttgcaaaa
    13501 acggccgcta tctgtgaagg gtcggtatct tttgaaccta atccgtatct gccgcccact
    13561 attaccggag cattcttatc tcccttgtat gcggctacaa catccagata caacggctcg
    13621 cctgttgcac ccggctcttt tgttctgtcc agaaccgcta ttttcttgac cgtcttgggt
    13681 aatgcattaa gaaagtgctt aattgagaaa ggtctgtaaa gacgtacttt aagtaagcct
    13741 actttttcgc ctttttccgt taagtagtcg acaacttctt cagctgcatc ggtaacgctg
    13801 cccattgcaa ctataactct gtcggcatct tttgcgccgt agtaatcaaa caggttatat
    13861 gtcctacccg ttattttgga aattttcttc atatagtctt ccactatagc gggaattgca
    13921 tcgtagaatt tgttaaccga ttcctttgcc tggaagaata tatcgggatt ctgtgctgtt
    13981 cctcttaaga ccggcttaga cggatttaat gctctttgtc tgaatgcctt aatcgcatcc
    14041 ttgtccacaa gctcttcgaa atccgcatag tcgataactt ctattttctg aatttcgtgg
    14101 ctggttctga atccgtcaaa gaaatgcacg aaaggcactc tacccttaat tgcggcaagg
    14161 tgtgctactc cggcaaggtc cattacctcc tgaacaccgc ccgatgcaag cagtgcaaaa
    14221 cctgtctgtc ttgtggcgta tatatccgaa tgatctccga atatggaaag tgcatggccc
    14281 gctacggctc tggcactgac atggaatact gccggtaaca gctcgcccgc aattttatac
    14341 atattgggta tcataagtaa taatccctgt gatgccgtat atgttgttgt taaagctccc
    14401 gcctgtaaag agccgtgaac ggtaccggat gctcccgctt ccgactgaag ctcgacaacc
    14461 ttaacttcct gaccgaacat atttttctga ccgtgagccg cccattcatc tacgttttcc
    14521 gccatgggcg acgagggtgt tatgggatat attgcggcaa catcggtaaa tgcatatgac
    14581 acatatgacg ctgcctggtt cccatccatc gttttaagtt tctttgccat ttgtctctcc
    14641 tttatttaac atgtatatta attaataact tggttgttac cacattgtat tgtagcacat
    14701 ataagcaatc ttttgtactc tactctacaa aatttctcct tattccatac tatactcagc
    14761 ctatggtata atcatgtcga aggaagcttt gctaccggga gaaataaatg aaaaaatcaa
    14821 aatacagatg gatatatatt gttgcctcta taatagttgc tttagtgacg gtctttctga
    14881 tattttcggc agagagacaa cataactttg aagcaatgga tcccaacggc agcgatgcga
    14941 aatggctcat taaccaaccc gtcgctcaca gggggtacta taacgaaacg accgctcccg
    15001 agaattcgtt ggaagctttt tcggaggcga taaaaaaagg gtatgctatc gagctggatg
    15061 tgcggctgac aaaggataat catgttatcg tttttcacga taagaatctt aaaagaatga
    15121 cgggttttga ttccgatgta aacatgacaa ccttagaaca gattaaaact cttacgttac
    15181 tggaaacttc cgccactata cccgaactgg tcgaagcttt agagttcatt aacggttccg
    15241 tgcctatatt actggagata aaatcctcag gattatctca taatttggat aagctcgttt
    15301 attcggttat aaaggagtat gaaggaagta ttgccgtgca gtctttcaat cctttaagta
    15361 taagatggtt tgcaaaaaat gctccgagta ttgccagggg tatactgctg agaggtaatc
    15421 ttaaatatgc tatcgcaaac agatttaagg ataatcttca tatgttattc tccaaaccaa
    15481 attttgtagc atacaaatac gagaaccttg ttaaaaccga tttttcccaa ttaagaaagg
    15541 acggcctcat agtactgggg tggacggtag gagaaaacat gcttaacaca agagaattta
    15601 ccgctcattg cgacaatatt atactggagg ctgaatgatg ctaaaaggaa gaaccctttt
    15661 aggatatgat gacaaaccgg ataaaaggaa gaatcttaag tacaccgatt ggctgattat
    15721 tattatcgcc ttatcaccga tatgggcgac tttgcttata gccgttgttg ttgcgatatg
    15781 tgcattgttt gccgcactgg tcttgggttt cacgctgacc ggcttcggtg cggtgacgct
    15841 ttttgtctat tccgcttttt ccttggcaga tgacctgtgg tcttccgtta tgaaaatggg
    15901 aatttgtctt tttgcttcgg gtgccgtaat aatcgctgtt gcgggatctt tcaaaggtat
    15961 aaaggtcttt ttcgtattat tgaaatcagg ttatatgaag attaaaaata tgctgttgtt
    16021 gaaaaaactg gagggtcaga aaaatgagca gaacccttaa ggccatcata atcggcggag
    16081 tatttatgct tttaggactg gtaatattcc tggtcggtta cttttccaac aatcgtaaag
    16141 cggatagttt acttgaattt gaaatccggg aaacaacacc cctgtcatta aaatcctatg
    16201 atgccccgca acccgacagt attacaatga taagcataat aacaaagaga atgcctgtca
    16261 ctattcaatc tcacgaagaa caatatatcc gtttggagta ttacgaaaat cccgagcagt
    16321 catttgagat aacgcaagcg ggcgggaaac ttatcgtcaa gaaaaacgac cttgggtttt
    16381 ttaaagaaaa tatattatta aatctcaagt ctctattagt gaaagagaag caggacaacg
    16441 gtatcaccgt ttatatgcct tcatcttata ccggagaatt gttagtcagc actaccttcg
    16501 gcgatatctc tgtcaggaat gcccttgcct tttctaaaat aaaaaagatt gattttaaaa
    16561 attaccgtgc ggatataaag atgaattcac tatccggcaa tattctgaac ctgatgaata
    16621 ccggcgggat aatagaatgc tcggacataa gtttcgaaga catcaccgtt atatccgcaa
    16681 atgcagatgt aaaactgaac tatatttctt ccgactatac cgacttctta ataaataaga
    16741 gttctctaca ggcagcaggt ataatatccg acaatatcaa aatcgagacg gtatcttcgc
    16801 agattgatat ggcaataaac gcatacagtg ttcaatattc ggtagtaatt gagaaatcgg
    16861 aaaaatcgca gataaatctg acggagaata ttgtcgagaa tgcggataag agaattcgca
    16921 tcaaagcgga tgactcaatt atttcggtaa gtttttctct ggattaacac ttcgcttcat
    16981 tgaaaataca tatatgccgt ggtatcattt acacattaaa agaaatggcg gtatcttaat
    17041 gtacagaata ttggaaagaa aagatttaaa cagtgatgtt gtctacatga aagtggatgc
    17101 tcccgctgtt gcaaagaaat gcgaaccggg acagtttata atacttcgtg tagatgaaac
    17161 gggagaacga atccccctga cgatatccga ctacaatcgt gaagaaggct gggtaagcat
    17221 aatttttcaa aaagtcggaa aaaccaccta ccttctggac acccttcagc aaggagatta
    17281 catactggat tttgtaggtc ctctgggaat tgcatcacac accgacaata tgagcagagt
    17341 ttgtgtagta ggcggaggtt taggttgtgc aatagcttat cctcaggcaa aaaaactctt
    17401 cggtatgggc cttcatgtcg atgtgatagc aggttttcgg aacaagtcgt tggtatttct
    17461 gcaagaagag atgaagcgta acagcacaaa ctactatctc atgaccgatg acggcactgc
    17521 aggagaaaaa ggtatggtta ccaaaaaact caaggaactt atagagggcg gagcaaacta
    17581 cgatacggta atagccatag gtcctgttgt tatgatgaag tatgtatcct tacttaccaa
    17641 agaatacaac attaagacta tagtgagcat gaatcccatt atgatagacg gcaccggaat
    17701 gtgcggagga tgcaggctga ccgttgacgg tcagaccaag tttgcatgtg ttgacggacc
    17761 ggattttgac ggacataaag tagatttcga cggacttatg caaagacttt ccatgtataa
    17821 ggaacaggaa aagcacaaat gtaatctgtt tagaggtatc gaataatggc aaaaataaat
    17881 atgactccgg agaaaacaaa aatgccggaa caggatcccg atatacgaaa ccgcaatttt
    17941 ctggaagttg caacgggata tactccccaa atggctcagg aagaagctca gcgctgtctc
    18001 aactgcaaac acaaaccctg tgtttccgga tgcccggtaa atgtgaacat tccctcgttt
    18061 atacaagcgg tggctcaggg cgatattgaa aaagcgtatg agattattac caaaaccaat
    18121 tcgcttcccg ctatctgcgg cagagtatgc cctcaggaaa cacaatgcga actgccctgc
    18181 gtgagaggca taaaaggaga accggttgct atagggagac tggagcgata tgttgcagac
    18241 tatatgcttg caaaagaaac ctttatagcc gacacaccta aacctacggt aaagaagaac
    18301 aaaaaagtcg cagtaatagg aagcggtcct gcaggcctta cggccgcatc ggacttatgc
    18361 gatatgggct atgatgtgac gatattcgaa gctttacaca aagccggcgg tgtacttgtc
    18421 tacggtatac cggaattccg tctgcccgaa tccatagtgg aaaaagagat tgaacgactc
    18481 aaggcaaaag gcgtatgtat agagttgaat atggtaatag gaaagataat ctccatacag
    18541 gagctgcacg aggaagaagg ttttgaagca atctttgtag gcacgggagc gggattgccc
    18601 aattttatga atataccggg tgaaaacctg aacggtgttt tttcggcaaa tgagttttta
    18661 acacgtgtca atcttatgaa ggcatatgat ccttcttacg atacccccat atatttcggc
    18721 agtcatgttg cggttgtcgg cggaggtaat gtggcaatgg atgccgcaag agccgcaaaa
    18781 aggttgggag cggaacaggt atatatcgta tacagaagaa gcgagcagga aatgcctgca
    18841 agagccgagg aagtacatca cgcaaaagaa gaaggtatag aattccgtct gcttacaaat
    18901 cccacaaaaa tattgggaga aaacggatgg gtaacaggta tggaatgcgt caggatgcaa
    18961 ctttccgaac ccggtcctga cggaagaagg cgacctgtgg ttatacccgg ttccgaatat
    19021 gttttaaaaa ccacttcggt gataatggca ataggcacca gccccaatcc tttgataaag
    19081 agcactaccg aaggtctgga aacaaacaag tggggttgca ttgttgcgga tgaggaaacc
    19141 ggacaaactt ccattccgta tatattcgcc ggaggggatg ccgtgaccgg tgcggcaacc
    19201 gtaatactgg ccatgggagc gggcaagaaa gccgccgccg ctattgataa atacctggac
    19261 aaatgatacc attgacagtg tattaacgaa gggagttgtt gatatgatca aaccttttga
    19321 aaccgtgctt cccgtaggaa aattatcaat aatcggaatg cgcggttgtg aagaaataac
    19381 aaaaaaagtt gattggtatc tgaaacaaag agccggctac aaggattccg accatacatt
    19441 tataaccgaa gcgatatgcc ccagattcgg aacgggagaa gctaaaggcg tcatactgga
    19501 atctataaga ggacacgacc tcttcataat ctgcgatgtt ttcaactacg gtgtaaccta
    19561 tgaaatgtac gggatgcagg tgcctatgag ccccgatgat cattatgccg atttaaaaag
    19621 aattatagcc gcaaccaacg gtaaggcaag aagaattacc gtcataatgc ctatgcttta
    19681 tgaaggcaga caacacaaga gaaaataccg cgaatcgttg gattccgcta tttctctgca
    19741 ggagcttgta ggcatggggg tggagaatat aattacattt gacgctcacg atcccagaat
    19801 tcaaaatgca atccccctga acggtttcga atcggttccg gttgcatatc agatgatgaa
    19861 aaagcttact gcatctttcc ccgacatcat atttgataaa gagcacacct tggtaatatc
    19921 ccccgatgaa ggcggaatga acagggcaat tccctttgca accatgattg gcttaaatct
    19981 cggtatgttc tataaaagaa gagatttttc cgttgtggag caaggcagaa accccataat
    20041 atcgcatgag ttcctcggag acagtgttga aggcaaagat gtaatagttg ttgaagacat
    20101 tatctcatcg ggcgaatctg cattggacat cttctatcag cttacaaaaa tgaaagcaaa
    20161 aagaatattt atgtgttgca ctttcggttt gttctgcaac ggtctcgaag cttttgacaa
    20221 agcttatgag gaaggtgcgg ttaccggagt acttaccacc aatcttgtat acagaagtcc
    20281 cgaattactt aagagaccct ggttcaagga agtggatatg tccaaataca tgtctttcat
    20341 aatagatacc ttacatcatg acgcttccat gtccaacctt ctcaatccgg tagacagaat
    20401 agacaagctg atggaaaagg taaaaagcag acaactggaa gaaggattga taaaataagc
    20461 aactattctt atataaactg tgccagatag aacggtaaat aaattaaatt gccgttcttt
    20521 tttaaatctt gagtgtgaat aacatatttg gtgtttattc tgtttgagta tttatttgca
    20581 aactcatcca tagatctgtg tgttctgtaa tttgatgact ttacttcaat cggacttacc
    20641 ttattatctt tggaaagcag gaaatccacc tcaaaacttc ttttctgagt ttcgtcataa
    20701 aaagtataat aaaacagctt gtttccgttg aaagccaaag tttgagcaac aatgttttca
    20761 taaagtaccc ccagattttt tgacaacttg ttgttcaaca acttctcata gattatattg
    20821 tccgtgaaat ccttatcttt gaacatgcat gttaccatta agccggtatc cgccataaat
    20881 aacctgaaat gctccatact taaattcgtt gtcattccta cggacgggtc gtttgagtgg
    20941 taagaaatga gaaccgtttt agaggcaacc agttcggcaa tttcctcgga tatagtagcg
    21001 gctctttgat tctttaacac actggaaacc tggtatctgg aagagtgtct gcttaattcc
    21061 gagggtattg aatcaaaaag catggatatt tttccgcttg aatcaatttt ataaaagtct
    21121 tcttcatata actgaataat atctctctta acctcatcta caaatttgaa attgtttgtc
    21181 tctatatatg ctttaacagc ttgtggcata cctccgacta acatatacaa tctgaacttt
    21241 ctcattaaag ctctgttttg atcttgaccc ggtgaaattt tctcattata aaattttctc
    21301 aaaatgttgt ttgtggtatt gtctccgatt gcccataaaa actcctcaaa atccatcgga
    21361 aacatttcta tttttctttc ctcactcggt ataagaatgt gctccacatt tttctttatt
    21421 gatattaaag aacctgtttc tatatagtca tacctgccgt cttctacaag tttctttaca
    21481 gcttgtctgg ctttgggaca aaactgaact tcatcaaata ttatcaatga atttctttca
    21541 tgcaggtcct ctttgaattg cagttgcagt tgcaaaaaca gataatccaa atcactgata
    21601 tcgttaaata attcttttac tccggtcgga gcaaaggcaa aatctataag tatatatgat
    21661 ttgtactcat tctttccgaa ttccgtgaca atggtagact ttcctacacg tcttgctccc
    21721 tctatcagta aagctgttct gcctttcgat gtttctttcc actccaataa tttatcgtat
    21781 atctttcttt taaacatgat tttcctcttt tttcaacaat aataccgttg gctaaacaca
    21841 taatactaca gatacgcatt ataaaacaag cctttttctg caaatacgca ttacaaaaat
    21901 gtgaaatttc aacaaatacg cattagaata gtatcatggc cttaaacatc caagcggtat
    21961 cacatacaca ccgtcatctc tttgataagc catttgagtg tttgttaaca gcataaggaa
    22021 cgatggttta gtagcggata tatttgctaa ttttattaaa ttagcacttg ctttatcagc
    22081 ttcattttct cctaatttaa cttctattcc tccccatctt ccgtcattca actcaacaat
    22141 caaatccact tcaagtcctg ttgaatcgtt gtagtaatat acggttcctc cgatttcttc
    22201 cgcataaact ctgatatctc ttgttgcaat agattcaaat aaaaaaccga atgtttcaaa
    22261 atcgtttttc agtttatcag gagacaagcc taaagcggca actgctatgc tggggtctac
    22321 gaattgtctt ttggttcctt ttctcagtct tgttgaactt cttatattct gcgaccaagg
    22381 caaaacatct tctactatat atagcctttt taaggagttc atatatgtat caaatgtttt
    22441 gggatttaca ataacttcat aaatagcgga attggctgct aatgtagaaa tattccttgc
    22501 ataactttta ataatttttc taaatttctc tttgtcctta gtaattccgt caacttcgtc
    22561 tatgtctctt ttgataatac tttcaagtaa atctttagga atttccaatt gtaagtcttt
    22621 gtttaagtcc aggcttccgg gccaaccgcc tctgcaaata cagtgtgcta tattatcaat
    22681 tgtaatattt gacttaaccg ggactaatgg cggttgtgaa aacaattctt ttaaagaaat
    22741 aagtccgtta gattctccgg tttcatataa actcatagga cgcatgtaaa ccgagttaat
    22801 ccttcccgtt cccgtatgtg aagttgttat gttttgtttt ttggtggatc ccgtcagaag
    22861 gtaggcacct ttatgattat tttcatcaat atcaagacga ataaagtccc aaatttccgg
    22921 aacttcctgc cattcatcaa acaatatagg tttttcaccc tttaaaacct catcttttga
    22981 tatggtagcc aaaatttgat attgtttttt aatcaacggg tcatgcagtt ttataacagt
    23041 attggaaaaa ataccggctg ttgtagattt ccctgcccat tttgctcctt caataatgac
    23101 gccgccgcta taggacattt ttctctcaat ttgagattca atcagtcttt ttctgtaatt
    23161 tatagtgttc atcagaccac ctcataacta cattataatg ccctattacc aaaaaatcaa
    23221 ccattctatt accaaaaaat aatgcattct attaccaaaa attcttatat tcttcctata
    23281 tctgtacttt gttataatca attaaatcaa tttatagcta actttctgac ttttacaatg
    23341 caatccttta ccttttgtca aattcaataa tgggcttgtt tatagacaca agatttttgt
    23401 ttctctttct ctatatgctg acacttaaac cgggaattgt ttctttaccg gactaaagaa
    23461 ccttgctcag tctttccacc gcttctatag tgttctctct gcttccgaag gatgtcagtc
    23521 tgaaatatcc ttccccgttg tggccgaatc cgctgccggg cgttccgaca acacatgttt
    23581 catttagcaa caagtcgaag aattcccagg acttcaaatt attagggcaa ctcatccata
    23641 tatacgggga gttgtttccg cctgtgtgat atataccttt ttccgtaaga agagcggaaa
    23701 taagttttgc attgtccaga tagtatctga tgttatcttt gcactgcttc attccttctt
    23761 ctgtaaatac agcttcagca cctctttgta tgatatagga tactccgtta agcttagtac
    23821 tctgtctcct tacccacatg tcgcgtaagg aaaaaccttc cagtattaat tctttcttta
    23881 ttaccgtata agcacatctt gtaccggtaa atcccgcaag cttggaaaaa ctgcagaact
    23941 ctatggcgca cttttccgct ccttcaatct caaatatact tctcggcaat ttatcatcgg
    24001 ttatgaatgc ttcgtatgcc gcatcaaaaa gtattacgga attatgcttc aaggcatact
    24061 ccacccattt ctccagctgt gctttggaat atacgcttcc gcaaggattg ttgggtgaac
    24121 acagatatat catatcggca tgtcttttgt ccggcatggc aagaaaatga ttttctttgt
    24181 ttgcatcggc aaatataatt ttcctgccgt ccattacgtt ggtatccata tacaccggat
    24241 atacaggatc gggtatcatg actatattgt ctcgtgagaa aatctccgtt atattaccca
    24301 aatcgctttt tgcaccgtcc gatacgaata tctcgcttat atcaacattt acatttcttt
    24361 tcttgtaatg ttcggaaatc ttttcccgca aaaagtcata accctggtcg ggagcatatc
    24421 ctctgaaagt ttccgcttta cccatttcct cggatgccgc cttcaaagct tccactactt
    24481 tttttgccaa aggtaatgtt acatcgccta ttcccaggct aataactttc ctgtccggat
    24541 gcgccttctt atatgccgca acttttgttg ctatatctct gaaaagataa ctctgtttaa
    24601 gattgccgta gtttacattc ggttttgcca tatttcttct ccttttcaat gttatatgct
    24661 aatttcggta tcaaacacat gcacggcttc gcctttcata ataactctgc cgtcatataa
    24721 atatgttatc ttcaatgttc cgcctctcaa tatgacggac acttcgctgt gcctttcgca
    24781 tataccgtta agtacggcag caaccaccgt tgcacatgct cccgttccgc aggccatagt
    24841 ctctcccgaa cctctttcat agactctcat ttttaaggtg ctcctgtcta tgatttccac
    24901 aaactcggta tttatcctgt cgggaaaata cgaatgcctt tcaaaatccg gacctatatt
    24961 atccaaatcc agtctgtcca gagactgcat aagattgctt ttattatctt tgtcattcat
    25021 aaatatgacg cagtgaggat ttcccatgga tacacaggtc acattatact ttttcccatc
    25081 tacgacaaga ggtttgttta tatatgtttc gtccctgttt gagttgtcat aaaccatagg
    25141 tatttcttta cgtttcagat aagcaatacc catatccact tccaccgaat ctatttttcc
    25201 ttttttgtcg ggaaacagtt ttaagtgttt tattccgctt tttgtctcga tgttcaactt
    25261 taacttatcg gtaagtctgt tgtcatacac atattttgct acgcatctta tggcatttcc
    25321 gcacatggca ccttccgaac cgtccgagtt gaacatcctc atataaaagt gtgcaacctt
    25381 tgaaggttgt attagaacca gtccgtcgga tcctatccct ttatgcctgt cgctgagttt
    25441 aatcgccaaa gaagaagggt tgtcaatctt acattccata caattcacat atatgtagtc
    25501 gtttcccgct ccgtgcattt ttgtaaattt cataatattc catcctttgt ttaattatat
    25561 ataaaaatgc aggtttggta aatatttatt cttctacctt gagtttccgt actttttcac
    25621 ctaccgattt aaacatctgt gttttccatt g
//
LOCUS       DAOSOM010000028        24749 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_2029_length_24749_cov_5.588928, whole genome shotgun sequence.
ACCESSION   DAOSOM010000028 DAOSOM010000000
VERSION     DAOSOM010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 24749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24749
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2029_length_24749_cov_5.588928"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1645
                     /locus_tag="PLF14_07125"
     CDS             <1..1645
                     /locus_tag="PLF14_07125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HOT71098.1"
                     /translation="SFRTELEDLAFMHLYPEEYADLTMKMERDKEQRFNMIDSIKGDL
                     ESDLKNSNIEGHVEGRVKHLYSIYRKMISQNKSLDQIYDIFALRIIVNNVKDCYFALG
                     MLHENYKPIPGRFKDYIAMPKKNMYQSLHTTLINKNGRPFEVQIRTWEMHRVAEFGIA
                     AHWKYKQKIDDAEQSSSLEWISQWMEWQKETRDEDEFYSNLKNELFDMEVFVFTPKGD
                     VINLPQGSNTMDFAYSVHSAIGNKMVGAKVNGKMVPITYVLQTGDIVEIITSNTSKGP
                     SRDWLKLVKTSQARTKIKQFFRKERKEENIEKGKEQLYKEIRRQGLNPSELLKPQYYE
                     KVLERYNFPELEDVMCAIGYGGITSNKVISRLKEEYKRHNKEEPKLEIPEVPEIVKTY
                     KSTEGIIVRDIPNCLLRISKCCNPVPGDDIVGYITRGRGVSVHRADCINIINDEEFAE
                     RKIDVYWNMDSDDKYETRLEIKAYDRANLVTDIMKLLSEQKIQCNGIKARIATDGYAN
                     IHIIVMIRNKDEIAELNRKLRKINGIYEINRFSKRQKIR"
     gene            1642..2097
                     /gene="dtd"
                     /locus_tag="PLF14_07130"
     CDS             1642..2097
                     /gene="dtd"
                     /locus_tag="PLF14_07130"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013681133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOT71099.1"
                     /translation="MIAVIQRVKESSVYIDNEKYSEIKSGLNVLLCVTQDDSESDVYY
                     LADKIVKLRIFADEQDKMNLSLLDLNKELLVISQFTLAADTKKGNRPSFTNAAEPEKG
                     NRLYQLFVSYIKEKYDINPKTGVFAANMQVHIVNDGPVTVIIDSTQHRK"
     gene            2172..2756
                     /locus_tag="PLF14_07135"
     CDS             2172..2756
                     /locus_tag="PLF14_07135"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT71100.1"
                     /translation="MLSSNCYILISKDEATVIDPCIEPDLIVNRVDEYSAKIEKIIYT
                     HAHIDHVYYGDDLKKITGAKTYGHIGDKDLYGNVYKNGAVLFGMNKKFDVYDIELSDN
                     QVFKIGDKQAVVIHTPGHTMGSICIHVNNSLFTGDTLFYESVGRTDLGTGNTDMLIDS
                     IKNRIYTLPEETVILPGHGVKSTVGHERANNPFV"
     gene            2810..4309
                     /locus_tag="PLF14_07140"
     CDS             2810..4309
                     /locus_tag="PLF14_07140"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOT71101.1"
                     /translation="MAKIYDEISRTFSEYLLIPNLTTKDCYPDNISLKTPLTRFKAGE
                     EPEIYLNIPFTSAIMQSVSNDTLAIALAKRGGLSFIYCSQPIEEQAEMVRRVKKFKAG
                     FVISDSNLNPDCTLKDVLALKAKSGHSTIPVTHDGTSQGKLLGLITSRDYRISRDPLD
                     KKVTDFMTPFSKLITGKMGITLREANDIIWDHKLNCLPIIDEENNLRYLVFRKDYDEH
                     KENPMELLDHEKRYMCGAGINTRDYKERVPALVEAGADVLCVDSSDGFSEWQYDTIRF
                     VKETYKGKVLVGGGNVVDAEGFKYLADAGADFIKVGIGGGSICITREQKGIGIGQATA
                     VIETVKARNEYFKKTGIYVPICSDGGIVYDYHIAMALAMGADFVMMGRYFARFDESPT
                     KVIKIGNNYVKEYWGEGSERARNWQRYDVGTDTGMKFEEGVDSYVPYAGKLADNLDKS
                     IAKIKNTMSSCGSKNIKELHDTARISMVSSTSIVEGGAHDVILKDKTDF"
     gene            4309..7134
                     /gene="uvrA"
                     /locus_tag="PLF14_07145"
     CDS             4309..7134
                     /gene="uvrA"
                     /locus_tag="PLF14_07145"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020813893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOT71102.1"
                     /translation="MQKYIFVKGAKENNLKNIDVRIPKDKLVVLTGISGSGKTTLAFD
                     TIYAEGQRRYVESLSSYARQFLGLMNKPDVEYIEGLSPSISINQKTTSHNPRSTVGTI
                     TEIYDYLRLLYARIGIPHCHICGREISSQSIDQIIDSILLFPEGTKIMVNAPIVKAKK
                     GEFQKLFDSLTKQGYTRVIADSIQYDLNEDKIKLDKNKRHDITLIVDRLIIREGIRKR
                     LTESVETALNEADGIVLINKDLSEDILFSTKLACPDCSISIDDLTPAMFSFNSPLGAC
                     EKCTGLGVLLKADSDLIIPDRNISVLDGAFVVAGMSFEKGSMMYSIYKMLSKKFNFDM
                     ECSFNDLPLEVQNILLYGTDGNPLYVEYETKTRAFSANEPFEGILRMVERRYKETAHS
                     YMRAYYEGFMSDSACPECNGKRLKPEILAVTVSGININEVTQLSVNECIKFFDNLKLT
                     EMQQNIAEILLKEIKVRLKFLADVGLDYLTLSRNASTLSGGEAQRIRLATQIGSKLTG
                     VIYILDEPSIGLHQMDNIKLLNALKEMRDLGNTLIVVEHDIETITEADYVIDIGPGAG
                     IHGGEIVGEGSVQDLINSERSITGKYLSGELEIKRPEKYRPASDKFIEIYGARQNNLK
                     NIDVKIPLGIFVCVTGVSGSGKSSLINEILYKELAFVLNGKKIKAGEHDRIEGMNYLD
                     KVINIDQSPIGRTPRSNPATYVGVFNDIREIFAMTKESKARGYKKGRFSFNVKGGRCE
                     SCSGDGVKKIEMHFLPDVFVTCEVCKGKRYNRETLEVKYDGKNISDVLDMTIEEAYDF
                     FKNIPQIESKLRVLMDVGLGYIRVGQSSTTLSGGEAQRVKLAYELSKRSTGKTMYMLD
                     EPTTGLHIHDVKNLLKIIDRLIASGNSVIIIEHNLDVIKCADYIIDLGPGGGDMGGNI
                     IAEGTVDEIMNNKNSYTGIYLKKYLGL"
     gene            7211..9436
                     /locus_tag="PLF14_07150"
     CDS             7211..9436
                     /locus_tag="PLF14_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOT71103.1"
                     /translation="MKLCDICKKNVAVVFVTRIQNNKHTQIGYCIPCAKTAGISPIDH
                     LIKNTGMSDEELGNINNQMGQFLQSLDFDDMVLPNNFRENILPSKSESSKKTKVKEPK
                     KDDKRKFLDAFGTNLNKKAMEGKIDNIIGRTKEIDRMIQILNRRQKNNPCLIGEAGVG
                     KTAIAEGLALKIVNQQVPAKLLNKEIYVLDMTGIVAGTQFRGQFESRMRNIINEVKEL
                     GNIILVIDEIHNIISAGDAEGGMNAANILKPALSRGEVQIIGATTIDEYRRHIEKDSA
                     LERRFQPIMVDEPSVQDTIEILHGIKKYYEQFHFVTFSDEAIRQAAILSERYIPDRFL
                     PDKAIDVIDEAASRANLNNEALVKLQMLNNELDEILQEKESAASADSIDDYKRAAELK
                     EKECRVREDIQKYKDILDKTEVTVQDIADVIEMWTKVPVTKITQIESAALLDLEKKLE
                     KKIVGQKQAIHAVAKSVRRARSGIKVKKRPASFIFVGPTGVGKTYLAKILAQELFVNE
                     ESMIRLDMSEYMEKHSVSKLIGSPPGYIGYDETGQLTEKVRRNPYSLLLFDEIEKAHP
                     DVFNILLQILEDGRLTDSKGRVVKFENTIIIMTSNAGTSYKNTGFGYVQDQTDSSDRV
                     RDALKEIFRPEFLNRVDDIIVFTSLSEQEIFEIGKLMAEEYAEDLKEKNILLKVSDQA
                     VKHIIGENYDDKYGARPLRRSMQTNIEDVLSEMYIKNELKDGMMVMISVEDGTLKYEC
                     I"
     gene            complement(9500..11458)
                     /locus_tag="PLF14_07155"
     CDS             complement(9500..11458)
                     /locus_tag="PLF14_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71104.1"
                     /translation="MKKKTQKNTAKKSKTKTKPKKAISKFSYSMGKIILVILFVAVVA
                     LALYFILDGSLKLINKRHIYNEKSVDALVMAVEKNDYGKAVEIYEQLSEEDKISLHNN
                     QSFLTAVDNRFSDVLAYDYNNGKNDYKEENAYKFVKYFCSFLHPDTVRENVYYFFNTY
                     KMYNSPPYENYRNFIGILNELLKDGNFEEQINLYNNEAEVIKASREQYFKGKTYEEQK
                     DYNNAYLCYANVVIEDTYAYELAKEDIARIKGFMAKEILDQAKTFESEGDIENAYYTM
                     KSAPEILSDVPEVSEYKSYITELYQRANYVPYTGVVYNIFFHSLALYPDIAFSSSRGQ
                     ELFNIMTTRYEFKKTLEKLYEHGFILINATDLYEIYIKDGKEHLKIKEQLLLPEGKKP
                     LIMSFDNMACTHASVGFCKMLTLDENNNLASIVTIDGVDTLTYDGEHVLILYDFVKKH
                     PDFSYNNALAIIGMSGYESMFGYATADLNSPSRQSDLDKAKKVADKLKEMGYVFANHS
                     YYHYTKSSDIPDTYTDLQWLTYDTNLWKEHIEPVLGKTNIYITPGGKNYSVAKYIDGD
                     RTDPCYNYLVDAGYQIILSVGRGQFYANNIIGIANPRFFFGTSLYMDRYNIDGKSFYK
                     TDVVLEQVFGFPYSDIIDPIREKYKQSY"
     gene            11600..12280
                     /locus_tag="PLF14_07160"
     CDS             11600..12280
                     /locus_tag="PLF14_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016298615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT71105.1"
                     /translation="MNISLNYVESGTGDVLILLHGNSENYEIFKHQIDYFSSKYRVIA
                     IDTRGHGNSPRGDAPFTLNQFAEDLNDFFLQHKISKANILGFSDGGNIAMIFTMKYPE
                     KVSKLILNGANLNPDGVKGYFNVFLKFMYKIFKCLSKISKNSNKYLEMMSLMVNEPDI
                     DVNGLKKISVPTLVIAGTNDLIKEEHTRLIASAIPDSKLVFIKGDHFIVSKKPEEFND
                     AVNSFLNN"
     gene            12416..13531
                     /locus_tag="PLF14_07165"
     CDS             12416..13531
                     /locus_tag="PLF14_07165"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HOT71106.1"
                     /translation="MNCTATCSFGLESVLSAELKKMEFENIAAENGRVNFSSDLEGIA
                     KANINLRTAERIYIELAVFNAVTFDQLFDNTERIPFHKYIDRYGAIVVNVSSVSSVLK
                     SIPNCQSIVKKAIIKSLGKYYKIDTFPETGARYPVNVFIMKDNVRIYLDTSGSSLYKR
                     GYRKRGGEAPLKETLASAIVQLSYWNKNRILIDPFCGTGTILIEAAMILKNIAPGLYR
                     DFVSERWPVFSNNEWSKVRQFYKSVIKEDNKVRLYGSDIDSSVIAAARHNARKAKVSD
                     VINFSVRDFKDITVPSEKGVIICNPPYGERLEDESTAKMIYSDMGKKFEEFNNWSIYI
                     LAPEKIFEDAYGKKADKRRKLYNGKIICNLYQYFGTK"
     gene            13543..13830
                     /locus_tag="PLF14_07170"
     CDS             13543..13830
                     /locus_tag="PLF14_07170"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOT71107.1"
                     /translation="MMNRNELSIRLAQERNITKVQSDEIINTLFEIIRQALKDEGKVC
                     IKDFGTFLVKKRKGREVKAIGSEEVIEIPDILELKLRVSKSMKDYLNDQED"
     gene            13814..14656
                     /locus_tag="PLF14_07175"
     CDS             13814..14656
                     /locus_tag="PLF14_07175"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015390370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOT71108.1"
                     /translation="MTRKINSDIIIDSVARACIEAACILDKQAYNAIESSLLNEDNET
                     AKYVLSELLDNADIAERDCIPLCQDTGMVISFVEMGIKVQLEEPVEDLINKGIKKGYE
                     EGVLRKSVVKDPFERINTKDNTPAIVYIKQTEGDKLKINLMLKGFGSENMSFVKMLNP
                     SLSQNEVVDIISDEICNRSYNACPPLFIGIGIAGTMDYACVLSKKALLRPLNESNINP
                     VYSDMEERILDKLNKSNIGPAGYDGKTTALSVAILAYPTHIAGLPVAVTIQCHALRHR
                     EIIL"
     gene            14653..15168
                     /locus_tag="PLF14_07180"
     CDS             14653..15168
                     /locus_tag="PLF14_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013380005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HOT71109.1"
                     /translation="MIKIHTPFNDEILCKLKPFDKVLITGYMYIARDQAHKRMYMEGI
                     PFDAAGQCIYHCGPSEAKNNEVIGSAGPTSSYRMNEYMPYMFENGIRAFIGKGKVSDA
                     VVESIKQNRCVYFGAIGGLGAKLSKCIKKQRIIAYESLGTEALREIYVENFPAVVIIN
                     CEGESIYERDG"
     gene            15170..16381
                     /locus_tag="PLF14_07185"
     CDS             15170..16381
                     /locus_tag="PLF14_07185"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HOT71110.1"
                     /translation="MEKIKMPKPIVEIDGDEMTRIIWSMIKDKLLLPYIDLNTEYYDL
                     GLVNRNVTDDSVTVQCAYAIKKHKVGVKCATITPNAQRMGEYNLKSMWKSPNATIRGI
                     LDGTVFREPILVKNIAPTVSTWKKSIIIARHAYGDVYSAKEYRVTSAGKLKAEFVFKD
                     GKTEDIQGFDFDSEGIVQIMHNKDKSIESFARSCFNFALDRNMDLWFSAKDTISKQYD
                     HHFKDIFADIYEKEYADRFKKAGIEYFYTLIDDAVARVVRSEGGFIWALKNYDGDVMS
                     DMVSSAFGSLAMMTSVLVSPQGEYEYEAAHGTVTRHYYRHLKGEKTSTNPIATIFAWT
                     GALRKRGELDNSDRLVTFADNLEASCIESIEQGYITKDLQNLVTGEKNTVMNTEDFIN
                     KIAERLEEKVK"
     gene            16378..16578
                     /locus_tag="PLF14_07190"
     CDS             16378..16578
                     /locus_tag="PLF14_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HOT71111.1"
                     /translation="MKKITENTKIIEVLKMDPNTAEIFFEFGMHCIGCPHASGESLGQ
                     ATRAHGVDTALLIEKLNEYFSH"
     gene            16579..17064
                     /locus_tag="PLF14_07195"
     CDS             16579..17064
                     /locus_tag="PLF14_07195"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HOT71112.1"
                     /translation="MINIVLVEPEIPQNTGNIARTCAATGSKLHLVKPLGFDVSDAHL
                     KRAGLDYWQDVDIYYYDSLYEFLEMHKRDNMFFASTKAAKTYTEVIYPDDCYILFGKE
                     TKGLDEQLLRDNYEFAIRIPMNNSIRSLNLSNSVAIVTYEILRQRGFSQMQLYGNLKN
                     S"
     gene            17173..18186
                     /gene="gap"
                     /locus_tag="PLF14_07200"
     CDS             17173..18186
                     /gene="gap"
                     /locus_tag="PLF14_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOT71113.1"
                     /translation="MTVKIGINGFGRIGRLVFRSAIDNPKVEIRGINDPFLDVAYMAY
                     LLKYDSVHGQFKGTIETGENFLIVNGKKVMVYASMEPAGIPWKDCGAEYIVESTGVFL
                     TKEKAQGHITAGAKKVVMSAPSKDDTPMFVMGVNEDKYTKDMTFVSNASCTTNCLAPL
                     VKIIHDNFGVVEGLMTTVHATTATQKTVDGPSAKDWRGGRAAAANIIPSSTGAAKAVG
                     KVIPSLNGKLTGMSMRIPTINVSVVDLTVRLAKPTSYEDIKAAIKKASETNLKGIVGY
                     TEDAVVSTDFIGDSRTCIFDAKAGIPLNDTFVKLVAWYDNEWGYSCKIVDLIMHMAKV
                     DAK"
     gene            18239..19429
                     /gene="pgk"
                     /locus_tag="PLF14_07205"
     CDS             18239..19429
                     /gene="pgk"
                     /locus_tag="PLF14_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOT71114.1"
                     /translation="MAELKKKTVEDINVSGKKVIARMDFNVPLKDGVITDDKRIREAM
                     KTIKYLSDHGAKTILVSHLGRPKGEFNPKYSLAPIAARISELLGKPVTLAKDVIGEDA
                     KAKAAALKDGDVMLLENVRFHAEEEQNDKEFSKKLASMAEIFVNDAFGTAHRAHSSTA
                     GIADYLPAVCGFLIKKEIEIMGAALAHPARPFVAILGGAKVSDKIGVIESLLDKVDTI
                     LIGGGMAYTFFKAQGHDIGTSICELDKLDFANELIAKAKKKKVNLLLPLDTVVADEFK
                     NDAMKKTVDFNAIPADMMGMDIGEKTVKLFTKEILKAKTVVWNGPMGVFEFENFANGT
                     RKIAKAVADSGSVSIIGGGDSAAAIEILGYADKVTHISTGGGASLEFLEGKVLPGIDV
                     LEDK"
     gene            19426..20178
                     /gene="tpiA"
                     /locus_tag="PLF14_07210"
     CDS             19426..20178
                     /gene="tpiA"
                     /locus_tag="PLF14_07210"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006565428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOT71115.1"
                     /translation="MRKKVIAGNWKMNKTPSETAAFITELSDKVKQTKDIVICAVPFV
                     CIPAALKASKRTKIKIAAQNMHYEDKGAFTGEVSAKMLVDLGVQYVIIGHSERRQYYN
                     ETDATVNRKVLKAIKAGLKPIICVGESLEQRETGVTADFVRYQVGYALNGVSSEDMKN
                     IVIAYEPIWAIGTGKTATNEQANEVCRTIRQALNEKYGRKVANATYIQYGGSVNVKNA
                     AELFSMSDIDGGLVGGASLKSDDFCKIVNYKG"
     gene            20190..21509
                     /gene="eno"
                     /locus_tag="PLF14_07215"
     CDS             20190..21509
                     /gene="eno"
                     /locus_tag="PLF14_07215"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOT71116.1"
                     /translation="MKQYIEIEGVWAREILDSRGNPTVEVEVFVEGGYVGRAAVPSGA
                     STGAFEAVELRDGDKKVYLGKGVEKAVDNVNEIIAPELEGLNVLDQVYIDRLMMELDG
                     TENKGKLGANAILGVSLAVAKAASEALGLSLYEYIGGVNSKVLPVPMMNIINGGKHAD
                     NSVSCQEFMIMPVGASSFKEALRWCTEVFHTLKKVLKDKGYSTAVGDEGGFAPNLKTD
                     EEALQVIVEAIKKAGYKPYDQIRIALDPAATEMYEEAKAKGKEGCYYFWKTDRMFTVD
                     QLIDYWAEMCEKYPIISIEDGLAEEDWEGWKKLTDRLGDKVQLVGDDLFVTNTKRLAR
                     GIKGGVANSILIKVNQIGTLTETLEAIEMANRAGYTAVVSHRSGETEDTTIADIAVAT
                     NAGQIKTGAPSRTDRVAKYNQLLRIEEELDDVAVYPGMDAWFNIKAK"
     gene            21640..22926
                     /locus_tag="PLF14_07220"
     CDS             21640..22926
                     /locus_tag="PLF14_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71117.1"
                     /translation="MRKFLTSTILIWLIFALLSVSVGCVKNIHETDYITDSEKYTYEY
                     IPVKTDKVSIIKMNGRSIKCKCFQGLYTSDDFLIRYATEEDIEKLNIKIDRTDDETVK
                     KQPSVIFSLDSEEITLIKDSFTCSIRVNFSVIFEMEFEYINKDGKKYIIQTNHSDYLN
                     LIKIPNSNKYLVEKGGFLFSLDPDRESLDFYCHVQTNGYSQLKSEYDVWASNPYFNDN
                     GTVMIFYSGRADKETGKVWVKNIITGKENPVPNTTGYSRVLSWRDGRYAYITSPFGKV
                     EEIDTVNLTSKVIHDTKIFSKDTLGFIYPYVFVPDMPGGTHIIDIHDDGEVYHFDDIK
                     YAKCMEIERVNGSDLLLLKYLYPVSKNKWHSEAVVLDLSTRKICIISVPDEYSVSTFK
                     QHNDCLMLSIYKPEDDIIIQTAYFIPISGIELVDSD"
     gene            22959..24377
                     /locus_tag="PLF14_07225"
     CDS             22959..24377
                     /locus_tag="PLF14_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71118.1"
                     /translation="MRKIFRFIVILFILLSMLFSYTGCQEKVKESSIPIETAGTETAV
                     VPVESDTVSTPNLEVTEEEQTDSQKEVVTDNTGTEVPGKPSRTYIDNMIFVNGKSLPV
                     FALPGVFRSLDGNLVRKPTNQDFKNLQASRDPVTHEITGSLPNEVLSVDGEEPVNADS
                     LVIYAGPYDYEFKYTYIEKDGRRQPVFSHGTDLQLTRIPMTNKYAVVKGGFLFWVDLD
                     AETMDLYCLSETMGYSMYDNFWEIDGIKYLKVWAIRPSFNRDGTKMLFYTERATEEVG
                     YVWVKDTVTGEEKPVPNTPAFTRVLQWRENRYAYLQSFGKIIEIDTVELTARTVFDNE
                     EAGSNSFNMIYPYVFNSVLDKDRNIFSEIINLNDGSKRKFEDSKYQRCDNITVVPNTN
                     LLLLRYAYPQENNPIYHEAVILDLVTGKRCVISVPLEYNISSIYQYREYYVVNLYKYG
                     DINNVHDQTSYFIPRTEIRFTD"
     gene            24511..>24749
                     /locus_tag="PLF14_07230"
     CDS             24511..>24749
                     /locus_tag="PLF14_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017260069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOT71119.1"
                     /translation="MLIKNFKKDNLEVSVFDTRREMGLKAAQDVRSEILKLFKTKDEI
                     NMLFAAAPSQNDFLQTLISYKDIDWTRINAYHMDE"
BASE COUNT         8693 a         3911 c         5315 g         6830 t
ORIGIN      
        1 ttctttcaga acggaactgg aagatctggc ttttatgcat ctttatcccg aagaatatgc
       61 cgatctcaca atgaaaatgg agagagataa agaacaaaga tttaatatga ttgatagtat
      121 taaaggcgat ctggaatcgg atttgaaaaa cagcaatata gaaggccatg tggaaggaag
      181 agtaaaacac ctatacagca tataccgtaa aatgatttca caaaataaat ctctggacca
      241 gatttatgat atcttcgcac tacgtattat tgttaataat gtaaaagact gctattttgc
      301 tttaggaatg cttcatgaga attataagcc gatacccgga agattcaagg actatatagc
      361 aatgcccaag aagaatatgt atcaatcctt acataccacc ctcataaaca aaaacggaag
      421 accttttgaa gtacagataa gaacatggga aatgcacaga gtagccgaat tcggtattgc
      481 tgcccactgg aagtataaac aaaagattga tgatgcggaa cagtcatctt cattggaatg
      541 gatttcacaa tggatggaat ggcagaaaga gacaagagat gaagatgaat tttactccaa
      601 tttaaaaaat gaactttttg atatggaggt ttttgttttc acacctaaag gagatgttat
      661 caatttgcct cagggctcaa atacaatgga ctttgcatac agtgtgcatt ccgcaatagg
      721 taacaaaatg gtaggtgcaa aagtaaacgg taaaatggtt cccattactt atgtactgca
      781 aacaggggat attgtagaaa taataacatc caatacctca aaagggccaa gcagagattg
      841 gttgaagctt gtaaagacaa gtcaggcaag aacaaaaatt aaacaattct tcagaaaaga
      901 gcgaaaagaa gaaaatattg aaaaaggaaa agagcaatta tataaagaaa taagaagaca
      961 aggtcttaat ccgtcagaac ttcttaaacc gcagtactat gagaaagttc tggagagata
     1021 taattttcct gaattggaag atgtaatgtg cgcaatagga tatggcggta ttacttccaa
     1081 taaagtcata tccagactga aagaagaata taagagacat aataaagaag aaccgaaatt
     1141 ggagataccc gaagttccgg agattgttaa aacatataaa agcactgaag gtatcatagt
     1201 aagagatata cctaattgtc tgttaagaat cagtaaatgc tgcaatcccg ttcccggtga
     1261 tgatattgtc ggttatataa caagaggaag aggtgtatcg gtacatagag cggattgcat
     1321 aaatataatc aatgatgagg aatttgccga aaggaaaatt gatgtatatt ggaatatgga
     1381 ttccgatgat aaatatgaga cacgattgga aataaaagct tatgacagag ccaatcttgt
     1441 taccgatata atgaaacttt tatccgagca gaagattcag tgtaacggaa tcaaagcaag
     1501 aatagccaca gacggttatg cgaatattca tattattgtt atgatcagga acaaagacga
     1561 gattgccgag ctcaacagaa aactgagaaa aataaacgga atatatgaaa ttaacagatt
     1621 ttcaaagagg cagaagataa gatgatagcg gttattcaaa gagtaaaaga aagttccgta
     1681 tatatcgaca atgaaaaata ctcggaaata aagtcaggct taaatgtact gctctgcgtt
     1741 acacaggatg acagcgaatc ggatgtgtat tatcttgccg ataagatagt aaaacttcga
     1801 atatttgcag acgagcagga taagatgaac ctgtcacttc tggacttaaa taaggaactg
     1861 ttggtaataa gccaatttac attagcggca gatactaaaa aaggtaacag accttctttt
     1921 acaaatgcgg cggaaccgga aaaaggtaat agattatatc aactttttgt aagttacatt
     1981 aaagaaaagt atgatattaa tcctaaaacc ggagtatttg cagcaaacat gcaggttcat
     2041 atagttaatg acggaccggt aaccgttatt atagacagta cacaacacag gaaataacat
     2101 atgttttttg tataagaata ggagaaaata aaatagatac tgaattaaag ataataagaa
     2161 taaccagtcc gatgctttca tccaattgct atattttgat atcaaaagat gaagcaactg
     2221 taattgatcc gtgtattgag ccggatttaa tagtgaatag agttgatgaa tattctgcga
     2281 agatcgagaa gataatatat actcatgctc atattgatca tgtatattac ggtgatgact
     2341 taaaaaaaat aacaggtgct aaaacatacg gacatatcgg tgataaagac ttgtacggaa
     2401 atgtatataa aaacggtgcc gttcttttcg gaatgaataa aaaatttgat gtttatgata
     2461 ttgaactgtc ggataatcag gtatttaaaa ttggtgataa acaagccgtt gtaatacata
     2521 ctccgggaca tacaatgggc tcgatatgta ttcatgtcaa taactcactc tttaccggag
     2581 atacattgtt ttatgagtcg gtgggaagaa ccgatttagg tacaggcaat accgatatgc
     2641 tgattgattc tattaaaaac agaatttaca cacttcccga agaaacggtt atattacctg
     2701 gtcatggagt aaaatccacg gtaggacatg aaagagccaa taatcccttt gtctgattga
     2761 cactgtttgt tacaaatagt ataatcaaaa aaagtgtaag aggtgtgaca tggctaaaat
     2821 atatgatgaa atttccagaa cattcagcga atatttgttg atacccaatt tgactacaaa
     2881 ggactgttat cccgataata tcagtcttaa aacgccgttg accagattta aagcaggtga
     2941 ggaaccggag atatatttga atataccttt tacgtcggca atcatgcagt cggtttccaa
     3001 tgatactctt gcaatagcat tggctaaaag gggcggttta agcttcattt actgttcaca
     3061 acctattgaa gaacaggctg aaatggtaag aagagttaag aaattcaaag cgggcttcgt
     3121 tatatccgat tccaatctta atcccgactg tacattgaag gatgtattgg ctttaaaggc
     3181 aaaaagcgga cattcaacaa tacccgtaac acatgacggt acctctcagg gaaaactgtt
     3241 aggattgatt accagcaggg attacaggat atccagagat cctttagata aaaaagttac
     3301 ggattttatg actccttttt ccaaattgat aacgggaaaa atgggtatta ctctgagaga
     3361 agcaaacgat atcatatggg accataagtt aaattgtctc cctataattg atgaagaaaa
     3421 caatttacga tatcttgtct tcagaaaaga ctacgatgaa cataaagaaa atcccatgga
     3481 gctgttggac catgagaaaa gatatatgtg cggtgcaggt atcaatacaa gagactataa
     3541 agaaagggtt cccgcacttg ttgaagccgg tgccgatgta ctttgtgtgg attcgtctga
     3601 cggattcagt gaatggcagt atgataccat aagattcgta aaagaaacat ataaaggtaa
     3661 agtcttggta ggtggcggaa acgttgttga tgccgaaggc ttcaaatatc tggctgatgc
     3721 gggagcggat tttattaagg taggtatcgg cggaggcagt atctgtatca caagagaaca
     3781 aaagggaata ggaatagggc aggctacagc ggtaatagag accgttaagg ccagaaatga
     3841 atattttaaa aagacaggta tatatgttcc tatctgttcc gacggcggaa tagtatatga
     3901 ctatcatatc gctatggcat tggccatggg tgcggatttc gttatgatgg gcagatattt
     3961 tgcaaggttt gacgagtcac cgaccaaagt aataaaaata ggaaataatt atgttaagga
     4021 atactgggga gaaggttccg aacgggcaag aaattggcaa agatatgatg tcggcaccga
     4081 tacgggtatg aaatttgagg aaggcgttga cagttatgtt ccgtatgcag gaaagttagc
     4141 tgataacctc gataaatcca tagcaaagat aaaaaatact atgtccagtt gcggttccaa
     4201 gaatatcaag gaactgcatg atactgcaag gatttccatg gtttcatcta caagtattgt
     4261 agaaggcgga gcgcatgacg tcatattaaa ggataaaacc gatttttaat gcagaaatac
     4321 atattcgtta aaggtgcaaa agagaataac cttaaaaata ttgatgtcag aataccgaaa
     4381 gataaattgg tagtgctgac cggtatatcg ggatcgggta aaacaacatt ggcttttgat
     4441 acaatttatg ccgagggtca aagaagatat gtagaaagtc tttcttctta cgcacgtcag
     4501 tttctgggct taatgaataa gcccgatgta gaatatattg aaggtttgtc accgtcaata
     4561 tccattaatc aaaaaaccac cagtcataat cccagatcaa ctgtaggtac tattacggaa
     4621 atatatgact atttacgact gctgtatgca agaataggca taccgcattg ccatatatgc
     4681 ggaagagaga tatcatcaca atccattgac cagataattg acagtattct gttatttccc
     4741 gaaggaacaa aaataatggt aaatgcaccg attgttaaag cgaaaaaggg cgaatttcag
     4801 aaactgtttg attcattgac aaagcaaggg tacacgagag ttattgcgga tagtatacaa
     4861 tatgatttaa atgaagataa aataaagctg gacaagaata aaagacatga tattactctt
     4921 atcgtagaca ggttgattat acgggagggc ataaggaaac ggttaaccga atcggtggag
     4981 accgcattaa atgaagctga cggaattgtt cttataaata aagatttaag tgaggatatt
     5041 ctgttttcca ccaagctggc ctgtcccgat tgttcaatta gcattgatga tcttactcct
     5101 gcgatgttct ccttcaacag tccgttaggt gcatgtgaaa aatgtacggg gcttggcgta
     5161 ttattaaaag ccgatagtga tttgattata cccgacagga atatttccgt ccttgacgga
     5221 gcatttgttg tagcggggat gtctttcgaa aaaggctcca tgatgtattc gatatataaa
     5281 atgctgtcaa agaagtttaa ctttgatatg gaatgttctt ttaacgattt acctttggaa
     5341 gtacagaata tattgctata cggaaccgac ggtaatccgc tgtatgtgga gtatgagacc
     5401 aaaacacgtg ctttttccgc caacgaacct tttgaaggta ttttaagaat ggtggagaga
     5461 agatataaag agactgcaca ctcatatatg cgtgcatact atgagggatt tatgagcgat
     5521 tcggcttgtc ccgaatgtaa cggaaaaaga ctgaagcctg aaattttagc cgttacggtg
     5581 agcggtatta atatcaatga agtaactcag ctatcggtaa atgaatgcat aaagtttttt
     5641 gataacctga agctaaccga aatgcagcag aatatagcgg aaatactttt aaaagagatt
     5701 aaggtaaggc tgaaatttct tgcagatgtg ggtttggact acctcacatt aagcagaaac
     5761 gcatcaacat tatccggcgg tgaagcacag agaattcgtt tggcgactca gataggctcc
     5821 aaactgacag gtgtaatata tattcttgat gaacccagta taggacttca tcagatggat
     5881 aacattaagc tgcttaatgc gttaaaagag atgagagatt tgggaaatac cttaatagtt
     5941 gtggagcatg atatagagac catcaccgaa gccgattacg tgatagatat aggtcccgga
     6001 gccggtatac acggaggaga gattgtggga gagggaagtg ttcaggacct cataaactcc
     6061 gagaggtcca taaccggcaa gtatctgtcc ggagaactgg aaataaagag acctgaaaag
     6121 tatagacctg caagcgataa gttcatagaa atatacggag cccgacagaa taatctgaag
     6181 aatatcgatg taaagatacc gttgggaata ttcgtgtgtg taacgggagt ctccggttcg
     6241 ggaaagagtt cattgataaa cgagatactg tacaaagagc ttgcctttgt tcttaacggt
     6301 aagaaaataa aggcgggaga acatgacaga atagagggaa tgaattatct ggataaggtt
     6361 atcaatatag atcagtcacc tataggcaga actcccagat ccaatcccgc tacatatgta
     6421 ggcgtattca atgatatacg tgagatattc gccatgacca aagagtccaa ggcaagagga
     6481 tataaaaaag gtcgtttcag ttttaatgta aaaggaggca gatgtgaatc ctgttccgga
     6541 gacggtgtta agaaaataga aatgcatttt ctgccggatg tctttgtaac atgtgaggta
     6601 tgcaaaggaa agaggtataa cagagagaca ctggaagtta aatatgacgg taaaaacata
     6661 tcggatgttt tggatatgac catagaagaa gcatatgatt tctttaagaa tattcctcag
     6721 attgaaagta aattaagagt gttgatggat gtgggactgg gatatataag agtggggcag
     6781 tcatcaacta cattatcagg cggagaagca cagagggtaa aacttgcata tgagttgtcc
     6841 aaaagaagta cgggaaagac aatgtatatg ttagatgaac cgacaacggg tttacatata
     6901 catgatgtga aaaacttatt gaaaataatt gacaggctta ttgcttccgg taattccgtt
     6961 ataattatag agcataatct tgatgtgata aaatgtgcag actatatcat tgatttggga
     7021 cccggaggcg gagatatggg aggtaatatt attgcggaag gtaccgttga tgaaataatg
     7081 aataataaaa actcctatac gggaatatat cttaaaaagt atctgggttt gtaaatataa
     7141 tattcacata aatttcacaa attaatggta gaatataaat aaccttgaat atgctgaagg
     7201 agaacataag atgaaattat gtgacatttg caaaaagaac gtggcagtag tgtttgtcac
     7261 aagaatacag aacaataaac atacacagat aggatattgt ataccttgtg caaaaacggc
     7321 aggtatatct cccattgacc acctcataaa aaatacggga atgtccgatg aggaattggg
     7381 taatataaat aatcagatgg gacaatttct tcaatcgtta gatttcgatg atatggtatt
     7441 gcccaataat ttcagagaaa atattctgcc ttccaagtcg gaatcatcca agaagacaaa
     7501 agtaaaagaa cctaaaaagg acgataaaag aaagttcctg gatgctttcg gtaccaatct
     7561 gaacaaaaaa gcaatggaag gaaaaataga taacataata ggcaggacca aagaaattga
     7621 tcgcatgatt cagatactta acagacgtca aaagaacaat ccttgtctta tcggagaagc
     7681 cggagtaggt aaaaccgcga ttgccgaagg tttggcactt aaaattgtaa atcagcaggt
     7741 acctgcaaag ttgttgaata aagaaatata tgtactggat atgacgggaa tagttgccgg
     7801 tacgcagttc cggggacaat tcgaatccag aatgcgtaat atcataaacg aagttaaaga
     7861 attgggcaac ataatacttg taattgatga aatacataat attatatcag ccggagatgc
     7921 cgaaggcgga atgaatgccg caaatatttt aaagcctgca ctttccagag gtgaagtaca
     7981 gataataggt gcaacaacca ttgacgagta cagaaggcat atcgaaaaag attccgcttt
     8041 ggaaagacgt tttcaaccta ttatggttga tgaaccgtcc gttcaagata ctattgagat
     8101 attacacggt atcaaaaaat attatgagca gttccatttc gtaacattct ccgatgaagc
     8161 aataagacag gcggcaatat tgtcggaaag atatataccc gacagatttc tgcccgataa
     8221 agcaatagat gttatcgatg aagcggcatc cagagcaaat ttaaataatg aagcattggt
     8281 taaactgcag atgttgaata atgaactgga tgagatatta caggaaaaag aatccgccgc
     8341 ttcagccgat tccatagacg actataaaag agctgcggaa ttaaaggaaa aagaatgtag
     8401 agtcagagaa gatatacaga agtataaaga catattggat aaaacggaag ttacggttca
     8461 ggatatagcg gatgtaatag agatgtggac aaaagtacct gttaccaaga taacacagat
     8521 tgaatccgcc gctttactgg atttggaaaa gaaactggag aagaagattg taggccagaa
     8581 acaagctatc catgccgtgg cgaaatccgt gagaagagca agatccggta ttaaagtgaa
     8641 aaagagacct gcttcattca tttttgtcgg accgacaggt gtaggaaaaa catatcttgc
     8701 aaagatactt gcacaggaac tgtttgtcaa tgaagaatca atgataaggc tggatatgag
     8761 tgaatatatg gaaaagcatt ctgtatccaa attgataggt tcaccgccgg gatatatagg
     8821 ttacgatgaa acaggccagt taaccgaaaa agtacgaaga aatccctatt cattattact
     8881 gtttgacgaa attgaaaagg cacatcccga tgtattcaat atactgttgc agatattgga
     8941 agacggaaga cttaccgaca gcaaaggaag agtagtgaag tttgaaaaca caatcataat
     9001 aatgacatcc aatgccggga catcatataa aaacaccgga ttcggatatg ttcaggatca
     9061 aaccgattct tccgacaggg taagggatgc tttaaaggaa atattccgtc cggagttcct
     9121 taacagagtg gatgatataa ttgtatttac aagtttaagc gaacaggaaa tattcgaaat
     9181 aggcaaactt atggctgaag aatatgcgga agatctgaaa gaaaagaata ttcttctgaa
     9241 agtcagtgat caagccgtaa aacatataat cggtgagaat tatgatgata aatacggtgc
     9301 aagaccgtta agaagaagca tgcagaccaa catagaagat gttctttccg agatgtatat
     9361 caagaacgaa ctgaaagacg gtatgatggt tatgatatcg gttgaagacg ggactctgaa
     9421 atacgagtgt atttaaatat ttacttataa gatttaatac tattataaat tgaatgaaaa
     9481 aatagttaag aaagtataat cagtaggact gcttatactt ttctcttata gggtcaatta
     9541 tatcggaata ggggaaaccg aatacctgtt caagtaccac atcggtttta taaaagcttt
     9601 taccgtcaat attgtatctg tccatgtata aggatgtacc gaagaaaaat ctgggattgg
     9661 cgatacctat aatattattt gcataaaatt gacctcttcc cacacttaaa ataatctgat
     9721 aacctgcatc cacaagatag ttataacagg ggtctgttct gtcaccgtca atatattttg
     9781 ccacggaata attctttccg ccgggtgtta tatatatatt cgtctttccg agcacaggct
     9841 caatatgttc cttccacaaa tttgtatcgt aagttaacca ttgtaaatcc gtataagtat
     9901 cgggaatatc ggaactttta gtgtaatgat aataagagtg attggcaaaa acgtaaccca
     9961 tctcttttaa cttgtcggca actttctttg ctttatccaa gtccgactga cgggatggtg
    10021 agtttaagtc tgccgtagca tatccgaaca tactctcata gccggacata cctattatag
    10081 ccagtgcatt gttataggaa aaatccggat gtttcttaac aaaatcataa agaatcagta
    10141 catgttcacc gtcataggtg agtgtatcca caccgtctat cgtaacgatt gaagccaagt
    10201 tgttattctc atccaaagtc agcattttgc agaaacctac acttgcatgt gtacaggcca
    10261 tattatcaaa ggacattata aggggttttt taccttcggg cagtaacagt tgttctttaa
    10321 tctttaagtg ctcttttccg tctttaatat agatttcata taaatcagta gcattaataa
    10381 ggataaaacc gtgctcatat aatttttcca gagttttctt aaattcgtat cttgttgtca
    10441 ttatattgaa taattcctga cctctagaag aggaaaaagc tatatcggga taaagggcga
    10501 gagaatggaa aaaaatatta tagacaactc ctgtatacgg aacatagttt gctctctgat
    10561 atagttcggt aatatagctt ttatactccg aaacttcagg tacatccgaa agtatttcgg
    10621 gagcggattt catagtatag tatgcatttt ctatatcgcc ttcgctctca aaagtttttg
    10681 cctgatcaag aatctccttt gccataaaac ctttgattct ggctatatct tccttagcaa
    10741 gttcataagc atacgtatct tcaataacaa catttgcata acacagatat gcattattgt
    10801 aatccttttg ctcttcatat gttttgcctt tgaaatactg ctctctggat gcttttatta
    10861 cttcagcttc attattataa agattaatct gctcctcgaa attgccgtct ttaagtaatt
    10921 cgttcaatat accgatgaag ttcctgtaat tttcataagg aggggaatta tacattttat
    10981 acgtgttaaa gaaatagtag acattttccc ttacggtatc aggatgtaaa aaactgcaga
    11041 aatattttac gaatttatat gcattttcct ctttataatc atttttccca ttattataat
    11101 catacgcaag aacatcggaa aatctattgt caacagcagt taaaaaagac tgattgttat
    11161 gcagggatat tttatcttcc tcagacagtt gttcataaat ctccacagct ttgccataat
    11221 cattcttttc tacggccata acaagtgcat caacggattt ttcattgtaa atatgtcttt
    11281 tgtttattaa tttcagcgat ccgtcaagta tgaaataaag ggctaaagcc acaactgcaa
    11341 caaaaagaat tacgagaata attttgccca tgctgtagct gaacttggat attgcctttt
    11401 tcggtttggt ttttgttttg cttttctttg cagtattctt ttgtgtcttc tttttcattt
    11461 ctttttcctt tgtatatcaa tttaataata ttttaccacc taataaagaa aaaataaata
    11521 tgcacgttat tgattcggaa aaaatctgaa caagaatgag aagatttatt tatgatataa
    11581 tcatccgggg gataaatata tgaatatatc attaaactat gtagaaagcg gaaccgggga
    11641 tgttttgatt cttcttcacg gtaacagtga aaactatgaa atatttaagc atcagataga
    11701 ttatttcagt tcaaaatatc gcgtaatagc tatagataca aggggacacg gaaattctcc
    11761 cagaggagat gcaccgttta cacttaacca atttgctgaa gatttaaatg atttcttttt
    11821 gcaacataaa atcagtaaag caaatatact gggcttttcc gatggcggta acattgcaat
    11881 gatatttaca atgaaatacc ccgaaaaagt ttcaaaactt attcttaacg gagcaaatct
    11941 gaatcctgac ggagtaaaag gatatttcaa tgtattctta aaattcatgt ataaaatttt
    12001 taagtgtttg tcaaaaatca gtaagaattc gaataagtat ctggaaatga tgagtctgat
    12061 ggtaaatgaa ccggatatcg atgtgaatgg tctgaagaaa atatccgtac cgacattggt
    12121 aatagcgggg acaaatgatc ttatcaagga agaacacaca aggcttatag catccgcaat
    12181 acccgattca aaattggtat tcataaaagg tgaccacttt attgtatcca aaaagccgga
    12241 agaatttaat gatgcagtta attcattcct aaacaattaa aaagattttc ctataatatg
    12301 tctgtcaaag tatacttttt taaatataat gacaattaac atttgcaata tagaataaaa
    12361 cgatataata tacgtaagcg tagaaattgt atcaattaat ggttaaggat taagtatgaa
    12421 ttgtacggcc acttgcagtt tcggtcttga atcggtgtta tctgcagaat taaagaaaat
    12481 ggagtttgag aatattgcgg cggagaacgg acgagtcaat tttagttccg atttagaagg
    12541 tattgcaaaa gccaatatca atttgcgtac agcagaaagg atatatatag aattggctgt
    12601 attcaatgcc gttacttttg atcaattatt cgataatacc gagagaatac cgtttcataa
    12661 atacattgac aggtacggag cgattgttgt taatgtttcc tcggtatcat cggtactgaa
    12721 aagcatacct aattgtcaat cgatagtaaa aaaggcaatt ataaaatcct tgggtaaata
    12781 ttacaaaata gatactttcc ctgaaaccgg tgcaagatat ccggtaaatg tgttcataat
    12841 gaaagataat gtaaggattt atctggatac atccggaagt tccctatata aacgaggata
    12901 cagaaaaaga ggaggggaag ctccgttaaa agaaacattg gcaagtgcca ttgttcaact
    12961 ctcttactgg aataaaaaca gaatactaat cgatcctttt tgcggaacag gaacaatact
    13021 tattgaggcg gctatgatat taaaaaatat tgctcccgga ttatacagag actttgtttc
    13081 ggaaagatgg cctgttttta gcaataacga atggagtaaa gtacgtcagt tttacaagtc
    13141 cgtgattaaa gaagataata aagttagact atatggttcg gatatagata gttccgtaat
    13201 agctgctgca agacataatg ctcgtaaagc aaaggtttcg gatgttatta acttttccgt
    13261 aagggatttt aaggatatta ccgttccatc ggaaaaaggt gttataatct gtaatcctcc
    13321 ttacggtgag cgactggaag atgaaagcac agcaaagatg atatattcgg atatgggtaa
    13381 aaaattcgaa gaattcaata attggagtat atatatactt gcacctgaaa agatatttga
    13441 ggatgcatac ggtaaaaaag cggataaaag aagaaaacta tacaacggaa aaataatttg
    13501 caatttatat caatatttcg gaacaaaata gtttggggga acatgatgaa cagaaatgaa
    13561 ttatcaatca gattggcaca ggaaagaaat atcacaaagg ttcaaagtga tgagattatt
    13621 aacacattgt tcgaaatcat caggcaagca ttaaaagacg aaggaaaagt atgtataaaa
    13681 gatttcggta cctttcttgt aaaaaagaga aaaggcaggg aagttaaggc gataggatct
    13741 gaagaagtta ttgaaattcc cgatatactg gaattaaaac tcagagtttc caaatcgatg
    13801 aaggattatt tgaatgacca ggaagattaa ttccgatata ataatcgact cggttgcaag
    13861 agcatgtatt gaagctgcct gtatattgga taaacaggca tataatgcca ttgaatcatc
    13921 tcttttgaat gaagataatg agaccgcaaa atatgtcttg agtgaactgc tggataatgc
    13981 agatattgca gaaagagact gtattcctct ttgccaggat accggtatgg ttatatcttt
    14041 cgtggaaatg ggcattaaag tgcaacttga agagcctgta gaagatttga ttaacaaagg
    14101 gataaagaaa ggctatgaag aaggggtgct caggaagtcg gttgtaaagg atccatttga
    14161 gagaattaac acaaaagata atactcctgc aattgtctat atcaaacaga cagaaggtga
    14221 taagttaaag attaacctta tgttgaaagg tttcggaagc gagaacatga gttttgtaaa
    14281 aatgcttaat ccttcacttt cacaaaatga agttgtagac attatatccg atgaaatatg
    14341 caacagatca tacaacgcct gtccgccctt attcataggt atcggtatag ccggtacaat
    14401 ggattatgcc tgtgtactgt ccaagaaagc gctgttaaga ccgttaaacg agagcaatat
    14461 caatcccgta tattccgata tggaagagag aattctggat aaattaaata aatcaaatat
    14521 agggcctgcg ggatatgacg gaaagaccac cgctctttcg gtcgcaattt tggcttatcc
    14581 gactcatatt gcaggattac ctgttgccgt tacaatacag tgtcatgctt taagacacag
    14641 agaaataata ttatgataaa aatacataca ccttttaatg atgaaatact gtgtaaatta
    14701 aaacctttcg ataaggttct tataaccgga tatatgtata ttgccagaga tcaggctcat
    14761 aaaagaatgt atatggaagg aatacctttt gatgctgcgg gacaatgtat ttatcattgc
    14821 ggaccgtcgg aagcaaagaa taatgaagtt atcggttcgg caggtcccac atccagttat
    14881 cggatgaatg aatatatgcc gtatatgttc gaaaacggaa taagagcatt tataggcaaa
    14941 ggtaaagtaa gtgatgcggt tgttgaaagc ataaagcaaa acagatgcgt atacttcgga
    15001 gctataggcg gtctgggtgc aaaactatcc aagtgcataa agaaacaaag aattattgct
    15061 tatgaatctt taggtacgga agctctgaga gaaatctatg ttgaaaattt ccccgctgtt
    15121 gttataataa actgcgaagg agaatcgatt tacgaaagag acggataata tggaaaaaat
    15181 aaagatgccg aaacccattg tagaaataga cggagatgaa atgaccagga ttatctggtc
    15241 aatgataaaa gataagttac ttttaccgta tatagacctt aataccgaat attacgatct
    15301 gggactggtt aacagaaacg taaccgatga ctcggttaca gtgcaatgcg cgtatgcaat
    15361 taaaaagcat aaagtaggcg ttaaatgtgc aacaattaca cccaatgcac aaagaatggg
    15421 cgaatataat cttaaaagta tgtggaaaag tcctaacgcg acaataagag gaatacttga
    15481 cggaacggtt ttcagagagc ctattcttgt taagaacatc gctcctacgg tttctacatg
    15541 gaagaaatcc ataataattg ccagacatgc atacggtgat gtttattcgg ctaaagaata
    15601 cagagttacg tcggcaggta aacttaaagc ggaatttgtt tttaaagatg gtaaaacgga
    15661 agatatacaa ggttttgact tcgattccga aggcatagtt cagattatgc ataataaaga
    15721 taaatccatt gaatctttcg ccagatcctg ttttaacttt gcactggaca gaaatatgga
    15781 tttatggttt tccgctaaag ataccatttc caagcaatat gatcatcatt ttaaagatat
    15841 atttgccgat atatatgaaa aggaatatgc agacagattt aagaaagccg gtatcgaata
    15901 tttctatacc cttattgatg atgccgttgc cagagtggta cgctcggaag gcggctttat
    15961 atgggcattg aagaattatg acggcgatgt aatgagcgat atggtttcat ccgcattcgg
    16021 ctcgcttgcc atgatgacat cggtattggt atcacctcaa ggagaatatg agtatgaggc
    16081 ggctcacggt acggtaacaa gacactatta cagacatctg aaaggtgaaa agacatctac
    16141 aaatcccatt gccacaatat ttgcctggac aggcgcatta agaaaaaggg gagagttgga
    16201 caactcggac agactggtaa cttttgcaga caatttagag gcatcctgta ttgagtcgat
    16261 agaacaagga tatattacaa aagatttaca aaatcttgta acaggtgaaa aaaacactgt
    16321 tatgaatacg gaagatttta ttaataaaat agcagaaaga ttagaggaga aagtaaaatg
    16381 aaaaaaatta ccgagaatac taaaataatc gaagttctta aaatggatcc caacactgcc
    16441 gaaatattct ttgaattcgg aatgcattgt ataggttgtc cccatgcaag cggagaaagc
    16501 ttaggacagg caaccagagc acacggtgtg gataccgcct tgcttattga aaagcttaac
    16561 gagtattttt ctcactgaat gattaatatt gttttggtgg aaccggaaat tccgcaaaat
    16621 accggaaaca ttgcaagaac ctgtgcagct acgggaagta agctgcatct tgttaagcct
    16681 ttgggttttg atgtatccga tgctcactta aaaagagcag gcctggatta ctggcaggat
    16741 gtggatattt attattacga ttcgctttac gagtttttgg aaatgcataa acgtgacaat
    16801 atgttttttg catctacaaa agccgcaaaa acatataccg aagttattta tcccgatgat
    16861 tgctatattc ttttcggaaa agaaaccaaa ggattggatg agcaactctt gagagataac
    16921 tatgaattcg ctatacgcat tcccatgaat aacagtataa ggtcgttgaa tttgtccaac
    16981 agtgttgcaa tagtaactta tgagatactc agacagagag gtttttcaca gatgcagctt
    17041 tatggaaatc tgaaaaatag ttaaaatttt aacaaattag tcgtaaaacc tcattttgca
    17101 tattgtaaaa ggcagaggaa tattataaaa tgatatggat aattaataga ttataattag
    17161 gaggaaaaat aaatgacagt aaaaattggt attaacggat tcggcagaat cggacgcttg
    17221 gttttccgat cggctattga caatcccaaa gtagagataa gaggaattaa cgacccgttc
    17281 ctggatgttg catatatggc atatctgctc aagtatgact cggttcacgg acaattcaag
    17341 ggtacaattg aaacaggcga aaatttcctt atagttaacg gaaagaaagt tatggtttac
    17401 gcatccatgg aaccggcagg aataccatgg aaagattgcg gagctgaata tattgtagaa
    17461 tcaacaggcg ttttcttaac aaaagaaaaa gcacagggac acattacggc aggagcaaaa
    17521 aaggtggtta tgtctgcacc ttcaaaagat gatacaccta tgtttgttat gggtgttaac
    17581 gaggataaat acacaaagga tatgaccttt gtttccaacg catcgtgcac aaccaactgt
    17641 cttgcaccgc tggtaaaaat tattcatgac aatttcggag ttgtagaagg acttatgacc
    17701 accgttcatg caacaactgc tacacagaag actgttgacg gaccttccgc aaaggactgg
    17761 agaggcggaa gagcggctgc cgccaatatc attccttcat caaccggtgc ggcaaaagcg
    17821 gttggaaagg ttataccttc acttaacggt aagctgacag gtatgtccat gagaatacct
    17881 acaataaacg tttcggttgt ggatttaacg gtaagattgg caaagcccac ttcttatgaa
    17941 gatatcaaag cggcaatcaa gaaagcatcg gaaaccaatc ttaaaggcat cgtaggatat
    18001 acggaggatg ctgttgtttc aaccgatttc atcggtgatt ccagaacctg tatatttgat
    18061 gcaaaagcag gaattccgtt aaacgatacc tttgttaaac ttgttgcctg gtatgataac
    18121 gagtggggtt attcatgtaa gattgtagac cttatcatgc acatggccaa ggttgatgca
    18181 aaataaaata acaaactaag gcccgtgaat acgggtcttt tttataaagg agactatcat
    18241 ggcagaatta aaaaagaaaa cagtagaaga tattaatgtt tccggtaaaa aggttatagc
    18301 cagaatggat tttaatgttc ccctgaaaga cggcgttata accgacgaca aacgaatccg
    18361 tgaagcaatg aaaacaataa agtatctttc cgaccacggt gccaaaacaa ttctggtatc
    18421 gcatctgggt cgtcctaaag gagagttcaa tcccaaatac tccctggcac ctatcgccgc
    18481 aaggatttcc gagttattgg gaaaacccgt tacacttgct aaggatgtaa tcggtgaaga
    18541 tgcgaaagca aaggcggcag cacttaagga cggagatgta atgttgctgg aaaatgtacg
    18601 tttccatgca gaggaagagc agaatgataa ggaattttcc aagaaactgg cttctatggc
    18661 cgagattttt gtaaatgacg ctttcggaac cgctcacaga gcacattcat caacagcagg
    18721 tatagcagat taccttcctg cggtatgcgg tttccttatc aaaaaagaga ttgagattat
    18781 gggtgcagct ttagcacatc cggcaagacc ttttgttgct attttaggcg gtgccaaagt
    18841 ttccgataag atcggtgtta tagagagcct tctggataaa gtcgatacta ttcttatagg
    18901 cggcggaatg gcatatacat tcttcaaggc acagggacat gatatcggaa catctatttg
    18961 cgaattggac aaactggatt ttgccaatga gctcattgca aaagcaaaga agaaaaaagt
    19021 taatcttctt ttaccgcttg acactgttgt agccgacgag tttaagaatg atgcaatgaa
    19081 aaagacagtg gatttcaatg ccataccggc tgatatgatg ggaatggata taggtgaaaa
    19141 aaccgttaag ttattcacta aggaaattct taaggctaaa acggttgtct ggaacggacc
    19201 tatgggagtt ttcgagtttg aaaattttgc caacggaaca agaaagatag caaaagcggt
    19261 tgccgattcc ggttcggtat ctattatcgg aggaggagac agtgcagcgg caatagagat
    19321 tttaggttat gccgataaag taacacatat ttccaccggc ggcggtgcat cattggaatt
    19381 cctggaagga aaagtactgc cgggtattga tgtattggag gacaaatgag aaaaaaagtt
    19441 atagcaggaa attggaagat gaacaagacc ccttcggaaa cggcagcatt tattaccgaa
    19501 ctttccgata aggtaaagca gacaaaagat atagttatat gtgcggtacc ttttgtatgt
    19561 atacctgccg ctttgaaagc atcaaaaaga actaagataa agatagcggc tcagaatatg
    19621 cattatgagg ataaaggtgc ttttaccggt gaagtttctg caaaaatgct tgtcgatttg
    19681 ggagttcagt atgtaataat cggtcactcc gaaagaagac agtactacaa tgaaaccgat
    19741 gcaacggtaa acaggaaggt tctgaaagct attaaagcag gtttaaaacc gataatctgt
    19801 gtaggtgaaa gcctggaaca gagagaaacg ggtgtaactg ccgattttgt cagatatcag
    19861 gtaggatatg ctttaaacgg tgtatcttcg gaagatatga agaacatagt tatagcttat
    19921 gaaccaatct gggcaattgg cacgggtaaa accgccacaa acgaacaggc aaatgaagtc
    19981 tgcagaacaa tcagacaggc tctcaatgaa aaatacggaa gaaaagttgc caatgcaaca
    20041 tacattcagt acggcggttc ggttaatgta aagaatgcag ccgagctttt cagtatgagc
    20101 gatattgacg gcggtcttgt aggcggagcc agtcttaaat cggatgattt ttgtaagata
    20161 gttaattata aaggataacg gaggtaaata tgaaacaata tatcgagatt gaaggcgtat
    20221 gggcaagaga gattctggat tccagaggaa atcccacggt agaagtggaa gtatttgtag
    20281 aaggcggata tgtaggaaga gcggctgttc ccagcggagc ttccacaggc gcttttgaag
    20341 cagtggaatt aagagacggc gataagaaag tttatttagg taagggtgtg gaaaaagcgg
    20401 ttgacaatgt aaacgagata atagcacccg aactggaggg attgaacgtt ctggatcagg
    20461 tgtacataga cagacttatg atggaactgg acggaacgga gaataaagga aagttaggtg
    20521 caaacgcaat actgggtgta tcattagccg ttgctaaagc tgcaagtgaa gctttgggat
    20581 taagtctgta tgaatatata gggggagtta attcaaaagt cctcccggtc cccatgatga
    20641 atatcattaa cggcggaaag catgccgata actcggtaag ctgccaggaa ttcatgatta
    20701 tgccggtagg agcatcttca ttcaaagaag ctttaagatg gtgtaccgaa gtattccaca
    20761 cattgaagaa agtattaaaa gataagggat actccacagc ggtaggtgat gaaggcggtt
    20821 tcgctcccaa tcttaaaacc gatgaagaag cattacaggt aattgtcgaa gcgataaaaa
    20881 aagcaggata caagccttat gatcagataa gaatcgcttt ggatcccgca gctacagaaa
    20941 tgtatgaaga ggcaaaggct aaaggaaaag aaggctgcta ctacttctgg aaaaccgaca
    21001 gaatgttcac tgtggatcaa ctgattgatt actgggcgga aatgtgcgaa aaatatccta
    21061 tcatttccat tgaagacgga cttgcggagg aagactggga aggctggaag aaacttaccg
    21121 acagactggg agataaagta cagctggtag gagacgattt attcgttacc aatacaaaga
    21181 gacttgccag aggtataaaa ggcggtgttg ccaactcaat actcattaaa gtaaaccaga
    21241 taggtacttt gaccgagaca ttggaagcta ttgaaatggc caacagggca ggctacacgg
    21301 ccgttgtaag ccatcgttcc ggtgaaacgg aagataccac cattgcagac attgcggttg
    21361 ctaccaatgc aggtcagatt aaaaccggtg ctccgtccag aaccgacaga gtggcaaaat
    21421 acaatcaact tctgagaata gaagaagaat tggatgatgt ggccgtatat cccggaatgg
    21481 atgcatggtt taatataaag gcaaaataac aaattaagcc gttatagtat atatgaatgt
    21541 aacggtgaag ataaaaaaga gaataaacaa ttatcattaa aggcacgatt aagtttgtgc
    21601 ctttttgatt ttcggatata atatatacgg agttatttta tgagaaaatt tttaacaagc
    21661 actattctga tatggctgat atttgcactg ttgtcggttt ctgtcggttg tgtaaaaaac
    21721 atacatgaaa ccgattacat tactgatagt gaaaaatata cttatgaata tattcctgtt
    21781 aagaccgaca aagtcagcat tatcaaaatg aacggaagaa gcatcaagtg taaatgtttt
    21841 caaggacttt atacatcgga cgattttttg atcagatatg cgacggaaga agatatagaa
    21901 aagctgaata taaaaataga tagaactgat gatgaaactg taaaaaaaca gccttccgtt
    21961 attttctcct tagatagtga agaaataact ttgattaaag attcatttac ctgtagtata
    22021 agggttaatt ttagtgttat atttgaaatg gaatttgagt atattaataa agacgggaaa
    22081 aagtatataa tacagacaaa ccattcagat tatttgaatc ttataaaaat tccaaattca
    22141 aacaaatacc ttgtggaaaa gggtggtttc ttattctcat tagaccccga tagagagtct
    22201 ttggatttct attgtcatgt tcaaacaaac ggatattcac aattaaagtc ggaatatgat
    22261 gtatgggcat caaatccgta ttttaacgac aatggaacgg ttatgatatt ttactcggga
    22321 agagcggata aagagacagg aaaagtctgg gtaaaaaata taataacggg aaaagagaac
    22381 ccggtaccta acacaacagg atacagcaga gttttaagtt ggagagacgg cagatatgcc
    22441 tatataacat cgccattcgg caaagtcgag gaaattgata ccgtaaattt aacttcaaag
    22501 gttatccatg acacaaagat tttttctaaa gatacactgg gtttcattta tccttatgtt
    22561 tttgttcccg atatgcccgg aggaacacat ataatagaca tacatgatga cggtgaagtt
    22621 tatcattttg atgatatcaa atatgcaaag tgcatggaaa ttgaacgagt aaacggctct
    22681 gatttattac ttcttaaata tctttatccc gtaagcaaga ataaatggca tagcgaagcc
    22741 gttgttttgg atttgtctac acgtaagata tgcataatat ctgtacccga tgagtattcc
    22801 gtttcaacat ttaagcaaca taatgattgt ttaatgttga gtatatataa gccggaagat
    22861 gatattataa tacagaccgc atatttcata ccgatatccg gtattgaatt ggtagattcg
    22921 gattgagtag tttcataaat aataaacgga gaaagaatat gagaaaaata tttagattta
    22981 tcgttatttt atttatcctg ttgtccatgt tattttctta tacaggttgt caggaaaagg
    23041 ttaaagaaag tagtattccg atagagacgg ccggtacgga aaccgcagtt gttcccgttg
    23101 agtcggatac ggtaagtacc cctaatcttg aagttaccga agaagagcaa acagactcac
    23161 agaaggaagt tgtaacggat aataccggta ccgaagtacc aggtaaacct tcaagaacat
    23221 atatcgataa tatgattttc gtaaacggta aaagcctacc cgtatttgca ttaccgggag
    23281 tattccgctc acttgacggc aatctggtca ggaaacctac aaaccaagat tttaaaaact
    23341 tacaggcttc aagagatccc gtaacgcatg aaataacggg aagtttaccc aatgaagtat
    23401 tgtcggttga cggagaagag cctgtaaatg ccgattcatt ggttatttat gcaggaccgt
    23461 atgattatga attcaagtat acatacattg aaaaagacgg tcgaagacaa ccggtttttt
    23521 ctcatggaac tgatttacag ttgacaagaa ttccgatgac aaataaatat gctgtggtga
    23581 aaggcggctt tttattctgg gtggatttgg atgcggaaac aatggaccta tattgtctca
    23641 gcgaaacaat gggatattcc atgtacgata atttttggga gatagacgga attaagtatc
    23701 tgaaagtgtg ggcgataaga ccttccttca acagagacgg aacaaaaatg ttattctata
    23761 ctgaaagagc tacggaagaa gtaggttatg tatgggtaaa agataccgtt accggtgaag
    23821 aaaaacctgt tccgaatacg ccggcattta caagagtact gcaatggaga gaaaacaggt
    23881 atgcgtattt acaaagcttc ggaaaaataa ttgagattga tactgttgaa cttactgcca
    23941 ggaccgtatt tgataacgaa gaagccggaa gtaattcctt taatatgatt tatccctatg
    24001 ttttcaattc ggttttagat aaagacagaa atatcttctc cgagattatt aatctgaatg
    24061 acggaagtaa aaggaaattc gaggattcga aatatcaaag gtgtgataat ataaccgtcg
    24121 ttcccaacac aaatcttctg cttttaagat atgcctaccc gcaagagaac aatccgatat
    24181 atcatgaagc ggtaattctg gatttagtta caggtaaaag atgtgtgata tcggttcctc
    24241 tggagtataa tatatcttca atttatcaat atagagaata ctatgtagtt aatctgtata
    24301 aatacggtga tataaacaat gtacatgacc agacatcata ttttataccg agaaccgaga
    24361 tacgttttac agattaacat tatctttatt tataaaatta attttttatt ttcaatcctg
    24421 ttttttgttt tattctgaaa aggcactatt ccgattgtgc cttttttgat ttgagatata
    24481 atagagatat atcaaattaa aggagttgtt atgttaatta aaaatttcaa aaaagacaat
    24541 ttggaagtat cggtatttga tacaagaaga gagatgggct taaaagccgc tcaggatgta
    24601 cgcagtgaga tattgaaatt atttaaaaca aaagatgaaa ttaatatgct ctttgccgcg
    24661 gcaccgtcac agaatgactt tttgcaaacc cttatatcgt ataaggatat agactggaca
    24721 agaataaatg cttatcatat ggatgaata
//
LOCUS       DAOSOM010000029        24434 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_2057_length_24434_cov_5.372575, whole genome shotgun sequence.
ACCESSION   DAOSOM010000029 DAOSOM010000000
VERSION     DAOSOM010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 24434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24434
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2057_length_24434_cov_5.372575"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..2524)
                     /locus_tag="PLF14_07235"
     CDS             complement(17..2524)
                     /locus_tag="PLF14_07235"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOT71120.1"
                     /translation="MKKICLNGWWDFYPVYEKDSCKEIPAQGWLEKAYLVPSLWRKPL
                     DCVKTPDEEYFREYRQEDLENIEELQFLYDDFGYPNTWTLTKEAWVRRYFDIECIEKE
                     KQYIILLEAVMPYSALYINGRKISSFVHPTLPYESDVTGYLKKGRNEVIVHIYDYEKD
                     EYGRYMTPTGTMSVADNAGIWQDVFLIERANVYVDDCFVKTYIDDMRIETDICIANSS
                     QRDRNIVVKSYIQDKLTKRKVLVFKDTSLYLRKNDIYDFTVSRCFPDAVLWEPSNPKL
                     YEFIIELYENENEIYKHKVNFGFREIKIEGKHIMLNNKPVHLFSDWGHKVTTYCYTKQ
                     WIESWFRMIKDGNMNHTRLHTCPHPSFILDMADETGILITGEAGLHGSGGYQASNSDE
                     YWINAEDHIRRFVKRDRNHPSLILWSVENEMRWNQKGKEQKYPEKTINNLPKLKQLIN
                     TMDPTRPAYHEGDSSLWDETKQDIISRHYGKDAVGADWWDQSKPLHIGEMALYHYEGP
                     NTALNLIGDKAYIDHRNVDISSALDAKMIIEYGRTGGVCCFGPWNQSCLKLIRTEKQD
                     RILEYKDFTVPGIKPLNVKAHTSEFAFWKRGKNYTVQKSFDIQKEAFRPFAVIDYSLR
                     NGYFKDELIKRTVYLVNDTSSDKAGTIIAKYGNDIVYKRNISIKKGYVEKSDIEFPAI
                     TDNDSYISHMKIAYEFTSENTLLDRQEIDICVYDMQSDEYMYYLKKINKNRITLFNDE
                     LFTDSISETGIQCINTDNLHSVNKQNTDILIITKNAIQDNDTIHRDIASLLDEGVKIL
                     LMEQKISIFKDIKIVNKPVLTAFKTNTKDKLKRVYDS"
     gene            complement(2530..5028)
                     /locus_tag="PLF14_07240"
     CDS             complement(2530..5028)
                     /locus_tag="PLF14_07240"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOT71121.1"
                     /translation="MSKKDNKKKKVHLICNAHIDPVWLWNWEEGAAATISTFRSAVRF
                     CKEYDEFVFNHNESLLYEWIEEYDPDLFEEIKELIKAGKWHVIGGWYLQPDANMPSGE
                     SFVRQIEEGRRYFMEKFGVDGGKTAINFDPFGHTIGLVQILKDAGYENYIVSRPAKLI
                     YDGSSLNDKHILWKGYNNKSVNVFKATTYGSRLGEFLATKYDYLYENQKDKKDIGMLW
                     GVGNHGGGPSKKDLDDIRGLMKQSDDEIVHTYPDKFFEEYLQRNQNDRVIKRSLHSFS
                     VGCYTSMVRVKQKHRELENKLLLCEKMMSHAVSDEALENYEHQMLREAWKNLLFCEFH
                     DALPGSSIQIVENDILKRLDHGLDIADRLIFRSYFRLVKGQKPAKEGEYPVLVYNPHP
                     YRVRTIVECEYCLAEQNRVPDTFSKGDVFDEEGKLVPCQNEKESSNIPIDWIKRVAFQ
                     ADLAPMSVNRFNIVNRLVEKKPFELPKTDENYIYFDNGTMQSKVNRKTGLIDSYKVNN
                     IEYIKPGMCRLLVIQDNTDSWGMTVSSYQKMKGKFKLATPSQAAKICGVDKKQPDSVR
                     VVESNELRTVVEASFVYSQSSCNIRYTFNKTDNIIGVNVRVFSQDKSVMYKLSIPTVL
                     TGAKCIGKTAYGRDILMTNGNENVSHQWLMLKKGTSAFAVINTGTYGSSCKRGEIRMS
                     VLRTPGYSAHPIGDRQILPLDRFSPRIDIGERLFDYYLWAGEYEEINENVDKIALINH
                     QKPIVLNFNPSDKGKKAKPFCLLDNETVELVTIRKCEDNEGYKIRLFNNLDKKNTCTI
                     KLPVFGYEKQVNFKPFEILTFICSPKAMKKTGLV"
     gene            complement(5045..6106)
                     /locus_tag="PLF14_07245"
     CDS             complement(5045..6106)
                     /locus_tag="PLF14_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT71122.1"
                     /translation="MELTGIERIARILKRQPVDRIGLYEHFWSDTHKAWGIETDIEER
                     LGFDMTELWAFSMVADLNHGRITVDEDEDTVTQIDGNYATLRKHKKHDATPEHVGFEI
                     DSREKWEERIKPLLTPSEQRIGFERYRAKKQYAKERNMFFVWSGTNVFELIHPLTGHV
                     NMLMAMIDDPEWVEDMCMTYAKLTVELQQILFEKEGYPDGIWYYEDMGFKNAPFISNA
                     MYDQFLYPAHKYTIDYAKSCNLPVIMHSCGFVEPLIPGMLKAGIDCLQVIEIKAGMDL
                     LRIHKNYKDCLSLMGGIDVRTLYTNDRVIIDKELESKIPIVKEGYGYVLHSDHSIPNT
                     VNLDTYEYFVKRGLELGKY"
     gene            6253..6696
                     /locus_tag="PLF14_07250"
     CDS             6253..6696
                     /locus_tag="PLF14_07250"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOT71123.1"
                     /translation="MTKQQQLNEALLNAIHRFTLSLRKKRISADIILHEFILLRKIQR
                     IQTEKNDLVYPSDLSCKSNLSKSYITAVLKSLEKKELIVRTLDASDRRRLPVRITDKG
                     YQIFANMAAKELEQADNLISALTEEKTLQLISLLNQSSDILDKGE"
     gene            6700..8427
                     /locus_tag="PLF14_07255"
     CDS             6700..8427
                     /locus_tag="PLF14_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012058319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT71124.1"
                     /translation="MKKLLKFLKKDWWKVALVLALTFAQSMANLYLPAYMSEIVNTGI
                     PTGDTVFILKTGGIMLAVALVGIICQIIARYTSANVSAKFAADLRDAVFTKVQSFSLS
                     EFDKFSTASLITRCTNDVSHVQRFFDMILRMAITAPMMAIGGIIMAIRYGQGLSWILV
                     IASIAIVMVMSLNFIFTIPKFNQMQVNIDKLNLIAREGLSGIRVIRAFNAQKTQDNKF
                     DDANVTLTKLHLAIGRIMSVLQPMTIFIMNITTLVALWLGASLVADSSIMVGDLMAFI
                     QYIMQILFSFQMLSMMFIIVPRAMVSVRRINEVSDIQPGIQDPSESDRKSPKSTGTIE
                     FRNVTFTYPGALEPALNNVSFTAKPNQMTAIIGSTGSGKTTLANLIPRLYDVTQGTVY
                     VDNVDVRQFSTHDLRSRIGYVPQKSMMFSGNIASNISFGRDISREDIAESADIAQATE
                     FINETEGGFNHSIAQGGVNISGGQKQRLSIARALAGSPKIYIFDDSFSSLDFQTESKL
                     KSALASKTLDSTVILITQRVSTIMNADQIIVLDAGKVVGMGKHNDLLSSCRVYYEIAS
                     SQLTKEELV"
     gene            8424..10298
                     /locus_tag="PLF14_07260"
     CDS             8424..10298
                     /locus_tag="PLF14_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT71125.1"
                     /translation="MTTQNQSDRRYDRPPQGRRGGGPRATMRLVEKPKDFKGAMKKLL
                     RYLKPYRIFLLLSVIFSMASTVFNILSPRQMGLITTELYVGMTQKNGRIDFNYIGRII
                     LILLLLYIGYTLFHVIQGLISNTVSQKIGYEMRKSMADKCNKLPLSYFDTNLHGDILS
                     RLTNDIDTITMTLNQSLSQITTTITQIVGVLVMMLLISPLMTAVAVLILPLSSVFVAV
                     LVKKSQKFFKAQQENLGDLNGHVEEMFSGHLVLKVFNGEERSIDKFKTFNAKLFESSR
                     KSQFLSGLMFPVTGFIGDIGYVAICVLGGYLVLSNGIQVGDIQAFIRYVRQFNNPLRQ
                     TAGIANTLQSTAAAAERVFEFLEETEEIPDSENIYNLSDFKGDVSFKNVYFGYDKNIS
                     IIKDFSVDVKAGQKVAIVGPTGAGKTTLVNLLMRFYDVDSGSISIDGVDIRDMKRNDL
                     RSLFGMVLQDTWLFNGTLRENIAYPDSDADFEDIQKAATLACAEHFILSGGDGYDMII
                     NEEASNISSGQKQLITIARTIMADNPMLILDEATSNVDTRTEILIQRAMDNLMLNRTS
                     FIIAHRLSTIRNADVIIVIDDGRIVEKGNHKELLSKQGFYHKLYYSQFDRVAANSIVS
                     "
     gene            complement(10285..11148)
                     /locus_tag="PLF14_07265"
     CDS             complement(10285..11148)
                     /locus_tag="PLF14_07265"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HOT71126.1"
                     /translation="MLSPKPVSHTNMEEIRDYFKYADKHMSLAQFSTTYLWRHSTGLL
                     YDIQDDFLYLFEKNYNYAAPLPLGNGDIYKALEKVKEFKQSVCGNNLVYCIGEDKVKL
                     FEDKYEVNELTDFFEYVYLTEDLINLSGRKFHGKRNHLNQFVSNNRYTYERITASDIP
                     LVMNLLDQWSRKKKYSVNLDVEKKAIYEILNYDIGVDYRAGAILTDNKVVAFAIGEQI
                     SDDMACVYFEKADTDIKGSYAVINNEFVKNEFSHLLYINRQEDLGISGLKKAKESYNP
                     VFKVKNYFMKL"
     gene            complement(11148..11870)
                     /locus_tag="PLF14_07270"
     CDS             complement(11148..11870)
                     /locus_tag="PLF14_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015253816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOT71127.1"
                     /translation="MTIRVTIWNEYLHEKNNPEAIKIYPEGIHETLKKHLSSEKDFVI
                     RTATLEQPEHGLTEEVLENTDVLIWWGHMAHGKVQDEVVQRVADRVRRGMGAILLHSS
                     HASKLMKALTGCSGRLLWRENNEKQIMWNLKPFHPVTQGIGEYIIIDGEETYGEPFGI
                     PNPDELLFISWFSGGNVFRSGWIKNIENGKLFYFQPGHETFPSYNNPQVIRVIKNAVR
                     YLKPAGFTEHTKSIWLKEPVVE"
     gene            complement(11870..12427)
                     /gene="efp"
                     /locus_tag="PLF14_07275"
     CDS             complement(11870..12427)
                     /gene="efp"
                     /locus_tag="PLF14_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOT71128.1"
                     /translation="MITAGDFRNGITFEMDGTVYQIIEFQHVKPGKGAAFVRAKIRNV
                     KSGSIVERTFNPSDKFELARMERKDMQYLYSDGDLFYFMDVETYDQIPINGETLGDTL
                     TFVKDNEVVQILSYKGEIFGVEPPNFVVLEVTHTEPGFAGNTAQGATKPATVETGAQI
                     KVPLFVNIGDLVRIDTRTGEYMERA"
     gene            complement(12584..12769)
                     /gene="rpmB"
                     /locus_tag="PLF14_07280"
     CDS             complement(12584..12769)
                     /gene="rpmB"
                     /locus_tag="PLF14_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOT71129.1"
                     /translation="MAKCEICDKSTGFGNKVSHSERKTGRTFKPNIRKVKINDNGTVR
                     RANVCTGCLRSDKVQRA"
     gene            complement(12892..13779)
                     /locus_tag="PLF14_07285"
     CDS             complement(12892..13779)
                     /locus_tag="PLF14_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71130.1"
                     /translation="MKYTKLCGKPDCSHNTKDCNAYIGISPIGYYNHKLYYFVRNTLF
                     TMNMDGNEHKRVKTLYEGMDGHFGYFHNGYYYFVITKGGSIGFSGNDDNNLYAVKVDD
                     SSKPVILLTDDIIKTITMFNLVGDNIYFFNYNLGNFELYSYSMITKELMKLGNKWSTY
                     GSCYFDDKFGYCYLPNEGFYEYDIYGKDLVNVKPVKFEDEGICSAFYYSDYIYLVHYC
                     GEKPSHENQILYIYDRKYNLVDSINFDKVVGKWSMGGFVTDVGDYILYASDFNDKPDY
                     YIDKSEIGTGELKFHKIEE"
     gene            complement(14028..16367)
                     /locus_tag="PLF14_07290"
     CDS             complement(14028..16367)
                     /locus_tag="PLF14_07290"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOT71131.1"
                     /translation="MKRRNITKILAVILIILLLMTQITACNKRRGVEKTVPEHVYRRT
                     EIALPEKIQYVSNMFLEGERIYVLGVGYNETDYTEYYILYSMNQDGSDPVEKKMDVSF
                     SKIDGYDGSYLSYITALSDQRLLAVVDAWAEDKEKGEYKSDNFFIILDKDGKVQKKIN
                     LNELLKNHITTDYFYAGMLLSDSAGNIYISSDRTIYVLKSDFTFAFKVELTGNSWMQS
                     MFNYSKDRIAVVMSTEGEEGYKMQIRIIDPEKKAFSEEINVNTHLQNNLYQLFTGLDY
                     SLYYSTQSGIYGYDVKSGESRELLNWINSDIENPNLGRMTAVSDKRFICITQEYDEST
                     WTSKQSVMILDYIPPEEVVPKYVITLATAFGAYDVRKPVIEFNRNSQEYRIQIKDYYQ
                     DHRDDEDYYAVIDKLNNDIIAGNMPDILLVDINMPFESYVSKGLLYDMYKLLNKDESF
                     NKEDYFTNVLDAMSVNGKLYSISPSFSVVTVAIKSKFVDTNKKGWTMGELQALMAKLP
                     KETETFIGTNRQEMMDMALSITLGRFIDKDSGKCYFDSQDFIDILKFTKGFNEKSFWE
                     DLDYNNLPADFWEKYDRSYEQDRAILNLVYLNSFYQYTQLRDYTFKEDITLIGFPNSD
                     RNGSAIGVENRIAISSKSKLIDGCWEFVKSFLSDKYQESITYQWPVKISAFDKMAEKA
                     MKGEDGYGPIRPLVGDVVYDSIGIPNPMPQEGRKISEEDVKYIKSFLQSVDVLMSYEQ
                     GVMDIINEEAKMYYAGSKTAEETAKIIQSRVQIYVSEGR"
     gene            complement(16345..17442)
                     /locus_tag="PLF14_07295"
     CDS             complement(16345..17442)
                     /locus_tag="PLF14_07295"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT71132.1"
                     /translation="MKTNTEDKKIISYRLIIFICILAVLIIAHLVMLSVSRKLKSNST
                     EQYAAERWMNNNSEINYAQISCFMTSDAGFTADNILSLNRSYDDRMLSESISSQTGAR
                     LWIHAYSGKGNVSVDADTNIPAQVTTIGGDFFYFHNIPLLSGNYFTDDPLNSDHVIIN
                     ESLAWQLFGSNDIIGKDIFINDVPYNIAGVSKDIHGENQAANPHIYMQYDVYQKMDNS
                     VFISCYEVLLPNPISDFALNIVKEYVRLNQMEHEIIQNTERFNLINTFKVLSNLKERN
                     IKTSKVLYPEWENTARITEYKLARLLSIRIIISVMMFTVLIVMIIVYRTNISDFFEKT
                     VKVIKTKAKNTKIAKAIEERRRKEYEKKEYH"
     gene            complement(17426..19450)
                     /locus_tag="PLF14_07300"
     CDS             complement(17426..19450)
                     /locus_tag="PLF14_07300"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOT71133.1"
                     /translation="MKTDVTKRRNIIKNIAIVFLAVMLVLTFFSNTILNYSLPEVAVQ
                     YPMYGTISSRIRGQGTVTANQTYQIYFDESRVIKSVDVRRGEIVQKGAVIYRLEDAPS
                     DELNNANNTLTTMYINYKRAMMQSTYDYSGILEEISELNKLLKEAVSIKTNLEINKLL
                     YADLQNQIRLAEKKTNELSVKQQELEQYLNSLKPDTSYSDEEFPVAYAIAKADYENAE
                     SMLNAEKENLKNLQRQLVAMANPVLEAQSAVDAKMQERDDYIALHPDTDIPTMAEIQA
                     KEDELEAAENEVPPDADKINRIKNELNGMIYRYFEYLPVREQIDEYNKQIANLTKALN
                     AVKNQERDINTAITFSRNRIDVLQFTHDDLYEIYLPLKARYEYGDKEAELVNVTAELI
                     SAQEELNALNERKSTVEAKVMTEAEADALVVQRQKELNDKQASYEKQKAQNELDEQIN
                     KMNLENMKREITQQEALVKRLQEKAVNGVITAPVTGVIMNLTYVTGEKTNAGAVVAEI
                     EISEKGYTMSFTVTIEQSRRLSVGQVAEVQYYYGSNAPYAEISAIKTDYNNPRGSRIV
                     ELEVRGDNIYPGQTMYVALGETGREYESVVPNSAIHEDNKGKFAYIIEAKNTPIGNRY
                     IARRADIQVIASDDSSSAISGVSAYANYIITTSSKPITDGMQVRLIENKY"
     gene            complement(19463..20380)
                     /locus_tag="PLF14_07305"
     CDS             complement(19463..20380)
                     /locus_tag="PLF14_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOT71134.1"
                     /translation="MNKAGIRNKDKIKRIISVAISMIILGYTALMFIMPVVLTVSNSF
                     MTKSEITANYGKVFSDSGTSAVSSYIADKVNLKIIPDKVSFGQYVTVLIKSPQYLLKF
                     WNSVIYVVPIVIFQIAISLGASYSFARYRGPFRNTLFFVYIALMLMPYQVTLVPNYLV
                     ADWMNILNTRWSIILPGIFSPFAVFILTKTMQRIPSSIVEAAKIDGANEWQIFSKVFV
                     PMSRNAIYSVALLLFIDYWNMVEQPLIMLQNNEDMHPLSVFLSKINAGEIGLAFAVAT
                     IYMVLPLLLFLYSEDRILEGIASSSAIKG"
     gene            complement(20373..21281)
                     /locus_tag="PLF14_07310"
     CDS             complement(20373..21281)
                     /locus_tag="PLF14_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOT71135.1"
                     /translation="MKRRDKTGEKRIKHVSFEKRDKTSTVPFMIPSIAGFLLFFLIPF
                     FIVLYYSFIDNPVNKEFVAFDNYVSLLNNHAFMLSLKNTLIFSVIAVPLAVLIPLMLA
                     LFLENNIPGKSIFRTALISPLVVPVASVILIWQVVFHYNGVINVFLESIGIEAVDWFK
                     SDFSRLVVVLLFLWKNTGYNMVLFMAALANIPVDVKEASLLDGVGPVRMFFRIKLNYL
                     SSSIFFVTILSLINSFKVFREVYLLTGDYPYEGLYMLQHYINNTFRTLDYQKLSAAAV
                     IICIIMTVIIAGLFVMDDKLGSSVDE"
     gene            complement(21458..22084)
                     /gene="nth"
                     /locus_tag="PLF14_07315"
                     /pseudo
     CDS             complement(21458..22084)
                     /gene="nth"
                     /locus_tag="PLF14_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517512.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
     gene            complement(22085..23491)
                     /locus_tag="PLF14_07320"
     CDS             complement(22085..23491)
                     /locus_tag="PLF14_07320"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HOT71136.1"
                     /translation="MVRTERNGKKEQNEELLFEDIKNNGYYMETIKTRKKSFVSRILL
                     PLFVCILCITLSVCATALTVFYVRKINQPDTDLLFLSNPTDPPEPVNTDIPVPAETQE
                     ADTDIEPESEKRENLVITTYRDISRVEVTPLEVDSNISDAVQKIMPSIAYIKCMYADE
                     KGVHMNEASALIISNDGYMITADSVIEPLCYDKSNKFKENCSITVFVDFDFNTLYFAK
                     VIGRDKTNNVALLKIDCDRPLQFIEYGDSDKLYLGETVVAVASGNNAFKGQVSAGIVT
                     GLSYTFNDRILSDAETPKDLMHMINTSTVMTGSNNGGALVNKKGQVVALTVFIKDEQQ
                     NGFFKAIPINKVKEASENLEVKSYNEETTPNLGIGISAEAWNFSITSEEDQKTIYVKG
                     IRVSYVGYGTPAFYSGLRVGDMIISINDVYVNSAENFIELKNTYLPGESLELTVYRRN
                     ETGDLKKLIITVTQDTVK"
     gene            complement(23543..>24434)
                     /locus_tag="PLF14_07325"
     CDS             complement(23543..>24434)
                     /locus_tag="PLF14_07325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT71137.1"
                     /translation="ALSVLLVAAFAKSVSGPLNDFLNAARSVSQGNLKVRAKYTDDRT
                     ELGELSKTFNSMMERLENTEQQRAEFTSNVAHELRTPMTSIGGFVEGILDGTIPQEKH
                     KTYLKRVSDEIKRLTNMVRDLLVLTKLDADDEASLVKNRFDINDMIRKSIMRFEEEIT
                     FKKLFINVNFHSELTYVYANSDDIERVLINLIHNAVKFTNDGGNITITVEPKRDKVYI
                     SIRDNGIGIEQEDLENIWTRFYKSDKARGRDGTGTGLGLPIVFSVIKRHKEMIDVKSK
                     KGIGTEFIFTLENAEQTDNS"
BASE COUNT         6892 a         5181 c         4190 g         8171 t
ORIGIN      
        1 caccccagaa acataactaa ctgtcatata ctcttttcaa tttatctttt gtatttgttt
       61 taaaagcggt aagaacgggc ttattaacaa tttttatatc tttaaatatt gatattttct
      121 gttccatcaa caatatcttt acaccctcat ctaacagtga agcaatatcc ctgtgaattg
      181 tatcattatc ctgtattgca ttttttgtta ttataagtat atcggtattt tgtttattta
      241 ccgaatgcaa gttgtccgta ttaatacatt gtattcccgt ctctgatata ctgtccgtaa
      301 acagttcgtc attgaatagg gtaattctgt ttttgttaat ctttttcaga taatacatat
      361 actcatccga ctgcatgtca tatacacata tatctatctc ctgcctgtcc aataaagtat
      421 tttccgatgt aaactcataa gcaattttca tatgggagat atatgaatcg ttatcggtaa
      481 ttgcgggaaa ttctatatcg gatttttcta catatccttt tttaatactt atatttcttt
      541 tataaacaat atcatttccg tactttgcaa tgattgttcc ggccttatcg gaagatgtgt
      601 catttacaag ataaacggta cgttttatca gttcatcttt aaagtatccg tttcttaatg
      661 aataatctat aaccgcaaaa ggtctgaatg cttctttttg tatatcaaat gacttttgaa
      721 cggtatagtt ttttcctctc ttccaaaagg caaattccga agtatgtgct tttacattga
      781 gaggtttgat accgggtacc gtaaaatctt tatactctaa aatcctgtct tgcttttcag
      841 tgcgtatcag tttaagacag gactgattcc acggaccgaa acagcaaact cctccggttc
      901 ttccgtattc tataatcatc ttggcatcca gagctgaaga gatgtcgaca tttctgtgat
      961 caatatatgc cttatctcct atcagattaa gcgcagtatt gggcccttca tagtgatata
     1021 acgccatttc gcctatatgc agaggttttg actggtccca ccagtccgca ccgacggcat
     1081 cctttccgta atgacgggaa atgatatcct gttttgtctc atcccacagc gaactgtctc
     1141 cttcatgata agcaggtctg gtcggatcca tggtgtttat aagttgtttc agtttcggaa
     1201 ggttattaat ggttttttcc ggatatttct gttccttacc tttttgattc caacgcattt
     1261 cattttccac actccacagt atcagcgaag gatggttacg gtctctcttt acaaatcttc
     1321 ttatgtgatc ttcggcatta atccaatatt catcgctgtt tgaagcctga taaccgcccg
     1381 aaccgtgtaa tcctgcttcc cccgttatca atatacctgt ctcatcggcc atatccagaa
     1441 taaaagacgg atgtggacag gtatgaagtc ttgtatgatt catattcccg tccttaatca
     1501 tacgaaacca gctttctatc cattgcttag tgtagcaata tgttgtgact ttatgtcccc
     1561 agtcggaaaa cagatgaaca ggcttgttat tcagcataat atgcttaccc tctatcttta
     1621 tttccctgaa tccgaaatta accttgtgtt tatatatctc attttcattt tcatacaatt
     1681 cgattataaa ctcatatagt ttcggattgg aaggttccca taatacggca tcagggaaac
     1741 agcggctaac cgtaaaatca tagatatcgt tctttctcaa gtataatgaa gtatctttaa
     1801 atacaagcac ttttcttttt gttaacttgt cctgaatata tgactttaca acaatgtttc
     1861 tgtctctttg agaagaattg gcaatgcata tgtccgtttc aatacgcatg tcgtcaatat
     1921 aagttttcac aaagcaatcg tcgacatata catttgctct ttcaataaga aatacatcct
     1981 gccagattcc cgcattgtcc gcaaccgaca tggtgccggt cggagtcatg taccttccgt
     2041 actcatcctt ttcatagtcg tatatatgca ctataacttc attacgtcct tttttcagat
     2101 atcctgtcac atccgattca taaggcagag tgggatgtac aaatgatgat atttttctac
     2161 cgtttatgta taaagcggaa taaggcatta ccgcttccag taaaatgata tattgttttt
     2221 ctttctcaat gcattcaata tcaaaatatc ttcttaccca tgcttcttta gtcaaagtcc
     2281 atgtattggg ataaccgaag tcgtcataaa gaaattgcaa ttcttcaata ttctccaaat
     2341 cttcttgtct gtactctctg aaatattcct catccggtgt tttaacgcaa tccagcggtt
     2401 ttctccataa ggaaggcaca aggtaggctt tttccagcca gccttgtgca ggtatctctt
     2461 tacatgaatc tttttcgtat accggataaa aatcccacca accgttaagg catatttttt
     2521 tcatgatatt tatacaagcc cggtcttctt cattgctttc ggtgaacaaa tgaaagtcaa
     2581 tatttcgaag ggtttaaagt tcacttgctt ttcatatccg aataccggaa gtttgattgt
     2641 acaggtattc tttttatcaa ggttattaaa cagtcttatc ttataccctt cattgtcctc
     2701 acattttctt atcgtaacaa gttccacagt ttcattgtcc aataaacaga acggtttagc
     2761 ctttttccct ttatcagagg gattgaaatt aagaacgata ggtttctgat ggttaattaa
     2821 ggcgatttta tctacatttt catttatttc ttcatattct ccagcccaca gataataatc
     2881 aaacagtcgc tcacctatgt ctatcctggg agagaatcgg tctaacggca atatctgtct
     2941 gtctccgata ggatgagcgc tgtaaccggg tgtacgtaac acactcattc ttatctcacc
     3001 tcttttgcag ctggaaccgt atgtaccggt gttgattacc gcaaaagccg atgtgccctt
     3061 tttcaacatc agccactgat gagatacgtt ttcattaccg tttgtcataa gaatatctct
     3121 gccgtatgcc gttttaccga tgcactttgc tccggtcaga acggtgggaa tacttaattt
     3181 gtacataaca ctcttgtcct gactgaagac tcttacatta acaccgataa tgttgtcggt
     3241 tttattaaat gtgtatctta tattacacga agattgagaa taaacgaaac ttgcttccac
     3301 gacagtcctt aattcgttgc tttccactac ccttaccgaa tccggttgtt tcttgtccac
     3361 accgcatatt ttagctgcct gtgagggagt ggcaagcttg aattttccct tcattttctg
     3421 ataggagctt acggtcatac cccacgaatc ggtgttatcc tgtattacca gtaaccggca
     3481 cataccgggc tttatgtatt caatattgtt gactttatag gagtctataa gaccggtctt
     3541 tcgatttacc tttgattgca ttgttccgtt atcaaagtaa atgtagtttt cgtccgtttt
     3601 cggcaattca aaaggttttt tctctactaa tctgtttact atgttaaatc tgttgaccga
     3661 catgggggct aaatccgctt ggaaagccac tctctttatc cagtcaatag ggatattgga
     3721 actttctttt tcattctgac acggtacaag tttgccttct tcgtcaaata catctccctt
     3781 agagaaggta tcgggtactc tgttctgttc ggcaagacag tattcgcact ctacaatggt
     3841 tctgactctg taaggatgag gattatacac taaaacggga tactcccctt ctttcgcggg
     3901 tttctgtccc ttaacaagac ggaaatatga tctgaatatc aatctgtctg ctatatccaa
     3961 tccgtgatcc aatctcttca aaatatcatt ttccactatc tgtatactgc tgccgggcaa
     4021 tgcatcatgg aattcgcaga atagaaggtt tttccatgcc tcgcgaagca tctgatgttc
     4081 ataattttcc agtgcttcgt cggaaactgc atgactcatc attttctcac ataaaagcaa
     4141 cttgttttcc agttctctgt gcttttgctt gactctcacc atggatgtat aacaacctac
     4201 actgaaagaa tgcagcgatc ttttaataac tctgtcgttt tgatttcttt gcagatactc
     4261 ttcaaagaac ttatcgggat atgtatgtac tatctcgtca tccgattgtt tcatcaaccc
     4321 ccttatatca tccagatctt tcttagacgg accgcctccg tggttgccca ctccccataa
     4381 cattccgatg tctttcttgt ctttttggtt ttcataaaga taatcatatt tagtggcaag
     4441 aaactctccc aatctggaac cgtacgttgt agccttaaat acatttacgg atttattatt
     4501 atatcccttc cataatatat gtttatcatt cagactgctg ccgtcgtata taagcttagc
     4561 cggtcgcgat acgatataat tctcataacc ggcatccttt aaaatctgca ccaagcctat
     4621 tgtgtgcccg aaaggatcaa agttaattgc cgttttccct ccgtcaacac cgaatttttc
     4681 catgaaatat cttctgcctt cctctatctg acgaacaaaa gattcaccgg acggcatatt
     4741 ggcgtcaggc tgaagatacc atcctcctat tacatgccat ttcccggctt ttatcaactc
     4801 ctttatttcc tcgaataaat cgggatcata ctcctcaatc cactcgtaca ataaggattc
     4861 attatgattg aaaacaaatt catcgtactc cttgcaaaac ctgacggcgg aacggaatgt
     4921 ggaaattgtg gcagccgctc cctcttccca gttccataac catacagggt caatatgcgc
     4981 attgcatatg agatgcactt tctttttttt gttatccttt ttactcataa atttcttcct
     5041 cccgttaata ctttcctaat tctaatcctc ttttaacgaa atactcatat gtatccagat
     5101 ttaccgtatt gggtatggaa tggtcggaat gcagtacata tccgtatcct tccttaacta
     5161 tgggtatttt gctctccaac tccttatcga taataactct gtcatttgtg taaagtgttc
     5221 ttacatctat tcctcccata agggaaagac agtctttata gttcttatga attctcagta
     5281 aatccatgcc ggcttttatc tctattacct gtaaacagtc gattccggct tttaacattc
     5341 ccggtataag aggttccaca aatccgcagg aatgcatgat aaccggaaga ttacaactct
     5401 ttgcatagtc tattgtatat ttatgtgcag gataaaggaa ttgatcgtac attgcattgg
     5461 atataaaggg agcgtttttg aaccccatat cctcataata ccagataccg tcgggataac
     5521 cctctttttc aaataatatc tgttggagtt ccactgtaag ttttgcatat gtcatgcaca
     5581 tatcttccac ccactcggga tcgtcaatca tcgccatgag catattgaca tgtccggtca
     5641 gagggtgtat caactcaaat acattggttc ccgaccagac aaaaaacata ttcctttctt
     5701 tggcatactg tttttttgct ctgtatcttt caaaaccgat acgctgctcc gaaggtgtaa
     5761 gtaacggctt aattctttct tcccattttt ctctggagtc tatttcaaac cccacatgct
     5821 cgggtgttgc atcatgtttt ttatgttttc tcagtgtcgc ataattaccg tctatctgtg
     5881 taactgtgtc ttcatcttca tcaacagtaa tccttccgtg atttaaatcg gcaaccatgg
     5941 aaaatgccca tagttccgtc atatcaaagc ccagtcgttc ttctatatcg gtttctattc
     6001 cccatgcttt atgtgtatcc gaccagaagt gttcatacag acctatcctg tctacaggtt
     6061 gccttttcag tatccttgca attctttcaa ttcccgttaa ttccatgtat aaacctcgtc
     6121 aggttaatta taacagaatg aatataaaat aatagtttaa acatatattt atattgactt
     6181 taaaaagagc ataaatataa ttcaatatat gaactattca ttttgtaaaa taattagttt
     6241 gaagatagga aaatgaccaa gcaacaacag ttaaatgaag cacttctgaa cgcaattcac
     6301 agattcacgt tgtcactccg aaaaaaaaga atatctgccg acattatact tcatgaattt
     6361 attctgctga ggaaaatcca aaggatacag acagagaaaa acgaccttgt atacccttcc
     6421 gatttgagtt gcaaatcaaa tctgtcaaaa tcctatataa cggctgtatt gaaatcttta
     6481 gaaaagaaag aactgatagt aagaacactg gatgcttcag acagacgcag acttcccgtc
     6541 cgtattaccg ataaaggata tcagatattc gccaatatgg ctgcaaaaga attggaacag
     6601 gcggataatc tcatatctgc tttgactgaa gaaaaaacat tacaattaat atcactgctt
     6661 aatcaatcca gtgatatctt ggataaagga gagtaaaact tgaaaaaact acttaagttt
     6721 ttaaaaaaag actggtggaa ggttgctctg gttttggcac tgacattcgc acaatccatg
     6781 gccaatctgt atctgcccgc ttacatgtcg gaaatagtca acacggggat acctacagga
     6841 gataccgtat ttatacttaa aaccggcgga ataatgcttg ctgttgcatt agtaggtatt
     6901 atatgccaga ttatagcacg atatacctcc gcaaatgtct ctgcgaaatt cgctgcggat
     6961 ttaagagatg cggtttttac taaagtccaa tccttttctt tatcggaatt tgataagttt
     7021 tccacagcct ctcttataac acgttgcacc aacgatgtga gtcatgtaca acgtttcttc
     7081 gatatgattc tcagaatggc catcaccgca cctatgatgg ctatcggagg tattattatg
     7141 gccataagat acggacaggg attatcctgg atacttgtaa ttgccagtat cgctatagta
     7201 atggtaatga gtttgaattt tattttcacc atacctaaat tcaatcagat gcaggtaaac
     7261 atagacaaac ttaaccttat agcaagagaa ggactgagcg gcatcagagt tataagagct
     7321 ttcaacgcac aaaaaactca ggataataaa ttcgatgatg caaatgttac tttgacaaaa
     7381 ctgcaccttg ctataggtcg tataatgtcc gtgctgcaac ccatgaccat atttataatg
     7441 aacattacca ctttagtggc attgtggctg ggagcatcac tggtcgcaga ctcatccata
     7501 atggtcggag accttatggc atttattcag tacatcatgc agatattgtt cagctttcag
     7561 atgctcagca tgatgttcat aatagttccg agagccatgg tatcggtaag aagaataaat
     7621 gaggtatcgg atattcaacc tggcatacaa gacccttcgg aatcagacag aaaatctcca
     7681 aaatctaccg gaacaataga atttcgtaat gttacattta catatccggg cgccttggaa
     7741 cctgccctca ataatgtttc atttaccgca aaacccaatc agatgaccgc aattatcggt
     7801 tcaacgggca gcggtaaaac cacattggct aaccttatac ccagacttta cgacgttaca
     7861 caaggtaccg tatatgtgga caacgtggat gtcagacagt tttccacaca tgacttacgt
     7921 tcccgtatcg gttatgtccc tcagaaaagc atgatgtttt ccggcaatat agcaagtaat
     7981 ataagtttcg ggcgagatat ttcccgtgaa gatatagcag aatccgccga tatcgcacag
     8041 gcaacggagt ttatcaatga aaccgaaggc ggcttcaatc actctatagc acaaggcgga
     8101 gtcaatattt cgggagggca aaaacagcgt ctgtccattg cacgtgccct tgcgggctca
     8161 cctaaaatat atatctttga tgacagtttt tcctcactgg attttcagac ggagtcaaaa
     8221 ctgaaatccg ctctggcaag taaaacactg gattctactg ttattcttat tacacagaga
     8281 gtcagtacta tcatgaatgc cgatcagata atagtacttg atgcgggaaa agttgtaggt
     8341 atgggcaaac ataatgattt attaagctca tgcagagtct attatgaaat agcttcatcg
     8401 caattgacaa aggaggagct ggtatgacga cacaaaatca gtctgacaga agatatgaca
     8461 gaccgccgca aggcagaaga ggcggcggac cgagagccac tatgagactg gtggaaaagc
     8521 ccaaagactt taaaggagcg atgaaaaaat tactcagata tcttaaacct tatcggattt
     8581 ttctgctttt atccgtaata ttttccatgg cctctaccgt ttttaatata ctttcgccga
     8641 ggcagatggg tcttataaca accgagttgt atgtaggaat gacacagaag aacggccgga
     8701 ttgattttaa ctatatcgga agaatcatat tgatacttct gctcctttat ataggttata
     8761 ccctttttca tgtaatacaa ggtctgatat ccaatacggt ttcacaaaag ataggatatg
     8821 agatgagaaa aagcatggcc gataaatgta ataaacttcc gctgagttac tttgacacca
     8881 acttacatgg cgatatactg agccgattaa caaatgatat cgataccata acaatgactt
     8941 taaatcaaag tttatcacag ataactacaa caattacaca aattgtcggc gtcttggtaa
     9001 tgatgttgct tatatcacct ttgatgacag cggtagcggt acttattctt cccctttcat
     9061 cggtttttgt tgctgtctta gttaaaaagt cacaaaagtt ttttaaagct cagcaggaaa
     9121 atctcggtga tttaaacgga catgtggagg aaatgttctc cggccattta gtactgaagg
     9181 tgtttaacgg tgaagaacgt tctattgata aatttaaaac attcaacgcc aaactctttg
     9241 aaagttccag aaaatcacaa tttttatcgg gacttatgtt tcccgttacc ggttttatag
     9301 gagatatagg atatgtggcc atatgtgtat taggcggata tctggtgtta tccaacggta
     9361 ttcaggtcgg agatattcag gcctttatcc gatatgtcag acaatttaat aatccgttac
     9421 gtcagacagc cggtattgca aatactttac aaagtacagc cgcggcagcc gagagggtgt
     9481 ttgaatttct tgaagaaacg gaagaaatac ccgattcgga aaacatatac aacttatcgg
     9541 attttaaggg tgatgtttcc tttaagaatg tttacttcgg ttatgataag aatataagca
     9601 taataaagga tttttccgtt gatgttaagg caggtcagaa agtcgccata gtaggaccta
     9661 caggagccgg taaaacaacc ttagtaaatc ttctgatgcg attttacgat gtggattccg
     9721 gaagcatttc cattgacggt gtggatataa gggatatgaa acgcaatgat cttcgtagtc
     9781 ttttcggcat ggtactgcag gatacatggt tatttaacgg cactttgcgg gaaaatattg
     9841 cttaccccga ttccgatgcc gactttgagg atatacaaaa agctgcgaca cttgcatgtg
     9901 ccgagcattt catactgtcc ggaggcgacg gatatgacat gattatcaat gaagaagcca
     9961 gcaatatttc ttccggtcag aagcaattga taacaatagc ccgtaccatt atggcggata
    10021 accccatgct tattctggat gaagcaacat ccaatgtcga cacaagaacg gagatactca
    10081 ttcagagagc aatggataat ctgatgctca accgcacctc gtttatcata gctcacagat
    10141 tgtccactat aaggaatgcg gatgttatta ttgtaataga tgacggaaga attgtggaaa
    10201 aaggtaacca taaggaactt ctttccaaac aaggtttcta tcacaagttg tattacagcc
    10261 agtttgacag agttgccgcc aattctatag tttcataaag tagtttttta ctttaaaaac
    10321 gggattataa ctttctttag ctttcttaag accgcttatc ccgaggtctt cctgtctgtt
    10381 tatatataga agatgggaaa actcattttt gacaaactca ttattgatta cagcatatga
    10441 tcctttaata tcggtatccg ctttttcaaa atatacacat gccatatcat cggatatctg
    10501 ttcgcctatg gcaaatgcaa ccactttatt atccgtcagt attgctcctg ctctgtagtc
    10561 tacacctata tcataattaa gaatttcata tatggccttc ttttccacat ccagattgac
    10621 cgaatacttt tttttcctgc tccactgatc cagaagattc ataacaagcg gtatatccga
    10681 ggcggttatt ctttcatatg tgtatcggtt gttactgaca aactggttga gatgatttct
    10741 ttttccgtga aactttctcc ccgaaagatt tatcaaatct tcggtaagat atacatactc
    10801 gaagaaatcg gtaagttcat tcacttcata tttatcttca aagagtttta ccttatcttc
    10861 tcctatgcag taaacaagat tgtttccgca taccgattgc ttgaactctt tgactttttc
    10921 taaagctttg taaatatcgc cgttacccag aggcagcggt gccgcataat tatagttctt
    10981 ttcaaacaag tatagaaaat cgtcctgtat atcatacaga agaccggtac tgtggcgcca
    11041 aagatatgtt gtggaaaact gcgctaatga catatgctta tcggcatatt taaagtagtc
    11101 ccttatttcc tccatattgg tatgtgaaac gggtttagga ctgagcatta ctcgacaaca
    11161 ggttccttaa gccaaatact ttttgtatgt tcagtaaatc ctgcgggttt aagatatctt
    11221 acagcattct taataactct tataacttga ggattattgt atgacgggaa tgtctcatgt
    11281 cccggttgaa aatagaatag tttaccgttt tctatgttct ttatccaacc cgaacggaat
    11341 acattgccgc cggaaaacca cgatataaac agcaattcat cgggattagg aataccgaac
    11401 ggttctccgt aggtttcttc tccgtctata ataatatatt cgcctatacc ttgcgtaacg
    11461 ggatgaaagg gttttaagtt ccacataatc tgtttttcat tgttttcacg ccacaataat
    11521 ctgcccgaac agcctgtaag agctttcata agctttgatg cgtgtgatga gtgaagcaat
    11581 attgcaccca ttccccttct taccctgtcg gcaactctct gcacaacctc atcctgaact
    11641 tttccgtgtg ccatatgtcc ccaccatata agaacatcgg tattttccag tacctcttcg
    11701 gtaagtccgt gttccggttg ctccaaggta gcggtgcgta ttacaaaatc cttctcggat
    11761 gataaatgct tctttaaggt ctcatgtatc ccttcgggat aaatctttat agcttccgga
    11821 ttgttttttt catgtaaata ttcattccat atagtaacac gaatagtcat tatgctctct
    11881 ccatatactc accggtacgt gtatcgattc ttaccaggtc ccctatatta acaaacaaag
    11941 gtactttaat ctgagcaccg gtctccacag tagccggctt tgtagcgccc tgtgcggtat
    12001 ttcccgcaaa acccggctcg gtatgcgtaa cttccaaaac tacgaaatta ggaggttcca
    12061 caccgaatat ctcgcctttg tatgacaata tctgcacaac ttcattatct ttgacaaatg
    12121 tcagtgtatc gcccaaagtt tcgccgttaa tgggaatctg atcataagtt tccacatcca
    12181 taaaatagaa aagatcacca tcggaataaa ggtattgcat atcctttctc tccatacggg
    12241 caagctcgaa tttgtccgac ggattgaatg ttctttccac tatggatccc gattttacat
    12301 tacgtatttt tgctcttaca aatgcagcac ctttacccgg tttgacgtgc tgaaattcta
    12361 ttatctgata taccgttcca tccatttcaa atgtaattcc gtttctgaaa tcacctgctg
    12421 taatcataac tttcctccat aataagcatc ttacctgatt ttactctatc accggaatgg
    12481 tgtcaatatt ccggcatatc ccgtagtgta acttgatgaa cctttgtaaa gtaattaaac
    12541 tgaaaatgtg caaaaaaaaa gagctaactc tttttttaaa aaattatgct ctttgtactt
    12601 tatcggaacg taagcatccc gtgcatacat tagctcttct gacagttccg ttgtcattaa
    12661 ttttcacttt tctgatatta ggtttaaaag tgcgtccggt ttttctctca gagtgactga
    12721 ctttattacc gaaccctgtt gatttatcac atatctcaca tttagccatt tgtctcacct
    12781 cctaaacagc acgcttatga attatacaat atgactaatc aataagcaag cattattttt
    12841 ctccttaatt tgcgcacagt ttgatatttg cagagactat aactttcttt ttcactcttc
    12901 aatcttatga aatttaagct cacctgtacc tatttcggac ttgtctatgt aatagtcggg
    12961 tttatcattg aaatcggaag catataatat atagtcacct acatctgtaa caaaaccgcc
    13021 catggaccat ttgccgacaa ctttatcaaa attaatgctg tctacaagat tatattttct
    13081 gtcgtatata tacagaattt gattttcgtg tgacggtttt tctccgcaat aatgaacaag
    13141 atagatatag tcggaataat aaaatgctga acatatgcct tcgtcttcaa atttgacggg
    13201 ttttacatta actaagtctt tgccatagat atcatactca taaaaacctt cattcggaag
    13261 ataacagtaa ccgaacttat catcaaaata gcaagatccg tatgttgacc atttattacc
    13321 taatttcatc aattcctttg ttatcattga gtatgaataa agctcaaaat taccaagatt
    13381 ataattgaag aagtaaatat tatccccaac taaattgaac atagtaatcg tttttattat
    13441 atcatcagtt aaaagaatta ccggcttgga actatcatca actttaacgg catacagatt
    13501 attatcatca tttccgctga atccgatgga gccgcccttt gttataacaa agtaataata
    13561 tccgttatga aaatatccga aatgcccatc catcccttca tataatgttt ttaccctttt
    13621 atgctcattt ccatccatat tcattgtgaa cagagtattt cggacaaaat agtacagttt
    13681 gtgattgtaa taccctatcg gcgaaatgcc gatgtatgca ttgcaatctt tcgtattatg
    13741 agagcaatca ggttttccac ataatttcgt gtatttcata tgcccatctt tagaataata
    13801 aaggtattcg ttaaatgatt tgtagaaaaa gccgtatgga gtttgaagaa acttgccaac
    13861 tgtaaaatcc gattgtctaa ataaagtata gtgctcctcg tttttatatg aaagtatgct
    13921 gaaagtttct tgaatagcaa cattattgca ggaagataag attcctgcaa caataataat
    13981 caatatggct aactgtattt ttttaatata gttcattatt ttttcggcta tctgccttca
    14041 cttacatata tctgcaccct gctttgtatt attttggcag tttcttccgc cgtcttgcta
    14101 ccggcatagt acattttcgc ttcttcgttt attatatcca taacaccctg ctcataggac
    14161 attaagacat caacactctg caagaatgat tttatatatt ttacatcctc ttcactaatt
    14221 ttccttcctt cctgtggcat cgggtttggt atgccaatgg aatcgtagac cacatctcct
    14281 actaaaggtc ttatcggacc gtaaccgtct tctcctttca tagccttttc cgccatctta
    14341 tcaaatgccg atattttaac cggccactgg tatgtaatac tctcctgata tttgtcggac
    14401 aggaaactct taacgaattc ccaacaaccg tctatcagtt tggattttga tgaaatagca
    14461 atgcggtttt ctacccctat ggcggaaccg tttctgtcgg aattgggaaa ccctattaat
    14521 gtaatgtcct ctttgaatgt ataatctctc aactgtgtat actgataaaa agagttgagg
    14581 taaacaaggt ttagtatggc tctgtcttgc tcatacgacc tgtcatactt ctcccagaaa
    14641 tccgcaggca gattgttgta gtccagatct tcccagaaag atttttcatt aaatcccttt
    14701 gtaaacttaa gaatatcaat aaaatcctga ctgtcgaaat agcatttacc ggaatctttg
    14761 tctataaacc tgcctaatgt aatacttaat gccatatcca tcatttcctg tcgattggtt
    14821 cctatgaagg tttcggtctc tttaggcagc tttgccataa gagcctgcag ttcgcccatt
    14881 gtccatcctt ttttattagt gtcgacaaat ttacttttta tagcaactgt aacaaccgaa
    14941 aatgagggtg atatggaata gagcttaccg tttacactca tggcatccag aacattggta
    15001 aaatagtctt ccttattaaa actctcatct ttattcaaaa gtttatacat atcgtaaaga
    15061 agaccttttg agacatagct ttcaaaaggc atattgatat ctacaagtaa tatatcgggc
    15121 atatttcccg ctatgatatc gttgttcagc ttatctataa ctgcgtaata gtcttcatcg
    15181 tcccgatgat cttggtagta atcctttatc tgaattctgt attcctgact gttcctgtta
    15241 aactctatta ccggttttct tacatcatat gctccgaatg ccgttgcaag agtaataaca
    15301 tacttgggca ccacttcctc gggcggtata tagtcaagta tcataaccga ttgcttactt
    15361 gtccatgtag attcatcata ttcctgagta atacagataa accttttatc ggaaactgcc
    15421 gtcattctgc cgaggttcgg gttttctata tccgagttaa tccagttgag cagttccctg
    15481 ctttcgccgc tcttaacatc atatccgtat atacccgatt gtgtggaata gtataaagag
    15541 tagtcgagac ctgtgaataa ttgatataaa ttgttctgca gatgggtgtt aacgtttatt
    15601 tcttcggaaa atgctttctt ttccggatcg attattctta tctgcatctt atatccctct
    15661 tcaccttcgg tactcataac aacagctatt ctgtctttgc tgtaattgaa catgctttgc
    15721 atccatgaat taccggtaag ttccacttta aaagcgaatg tgaaatccga tttcagtaca
    15781 taaattgtac gatctgacga tatatatatg tttcctgccg aatcggacaa aagcatgccg
    15841 gcatagaaat agtccgttgt aatatgattt ttaagcaatt catttaaatt gatttttttc
    15901 tgcactttac cgtctttgtc cagtattatg aagaaattgt ccgatttata ctctcccttt
    15961 tctttatctt ccgcccatgc atccacaaca gctaacagtc tttggtcgga taatgcggta
    16021 atatatgaca agtacgaccc gtcatagccg tctattttgg aaaaacttac atccatcttt
    16081 ttctctacag gatcggaacc gtcctggttc atactgtata gaatatagta ctcggtataa
    16141 tcggtttcat tgtatcctac acccaataca tagattcttt ctccttcgag aaacatatta
    16201 ctgacatact gtattttttc aggcaatgca atctccgttc tgcgataaac atgttcgggc
    16261 acggtttttt ccacacctct tcttttatta caggcggtaa tttgtgtcat caatagtaaa
    16321 ataataagta tgactgccag tattttagtg atattccttc ttttcatatt cttttctcct
    16381 tctttcttca atcgcctttg caatcttggt gttctttgct ttagttttta taaccttaac
    16441 ggttttctca aagaaatccg atatattcgt tctgtaaact ataatcatga ctataagaac
    16501 agtaaacatc ataactgata tgattatacg tatcgacagc aaccttgcca acttatattc
    16561 cgtaatcctt gccgtatttt cccactcggg ataaagtacc ttactggtct ttatattcct
    16621 ctctttcagg ttgcttaata ccttgaaggt atttatcaga ttgaatcgct ccgtattctg
    16681 aattatttca tgctccatct gatttaacct gacatattct tttacaatgt ttaaggcaaa
    16741 atccgatatg ggattaggca gcagtacttc atagcaggaa atgaaaaccg aattgtccat
    16801 cttttgataa acatcatact gcatgtatat gtgcggatta gccgcttggt tttccccgtg
    16861 aatgtctttg gaaacaccgg caatgttata cgggacatcg ttaatgaata tgtctttacc
    16921 tataatatcg ttggaaccga acagttgcca cgccaggctc tcgtttataa taacatggtc
    16981 actgtttaac ggatcgtcgg taaagtaatt tcccgataat aacggaatat tatgaaagta
    17041 aaagaaatct cctcctatgg tggtaacctg tgcgggaatg ttggtatcgg catccacact
    17101 tacatttccc tttccgctgt acgcatgaat ccataacctt gcacctgttt gcgatgatat
    17161 cgattcggaa agcatacgat catcataaga gcggtttaaa gacagtatat tatcggcagt
    17221 aaaaccggca tcggaagtca taaaacagga aatctgtgcg taattgattt cggaattatt
    17281 attcatccat ctctccgccg catattgttc tgtgctgtta cttttaagct ttctgctgac
    17341 tgataacatg acaagatgcg caatgataag cactgcaagt atgcatatga atataatgag
    17401 tctgtatgaa attatctttt tatcttcagt atttgttttc aatcaacctc acctgcattc
    17461 cgtcggttat aggtttcgac gatgtagtaa taatatagtt ggcataagca cttacacccg
    17521 atatagcgga agaggaatca tcggatgcta taacctgtat atccgctctt ctggcaatat
    17581 atctgttacc tataggagta ttctttgctt caataatata tgcgaatttg cctttgttgt
    17641 cttcatgtat ggcgctgttg ggtactaccg attcatattc ccttcccgtt tctcccagtg
    17701 caacatacat ggtctgaccg gggtatatat tatcccctct tacttccagt tccactattc
    17761 tgcttccgcg ggggttgttg taatcggttt ttattgcgga aatttccgca tatggcgcat
    17821 tggagccgta atagtactgt acttccgcaa cctgtcctac actcaatctt ctgctttgct
    17881 ctatagttac cgtaaaactc atggtgtaac ctttttcact tatctctatc tccgcaacca
    17941 ccgcccccgc attggtcttt tctccggtga catatgtaag attcataatc actccggtta
    18001 ccggtgcggt tataactccg ttaaccgctt tttcctgcaa tctttttacc agagcttcct
    18061 gctgagtgat ttctctcttc atgttttcca ggttcatttt atttatctgc tcatccaact
    18121 cgttctgagc cttctgtttt tcatatgaag cctgcttgtc attcagttct ttctgtcttt
    18181 gcacaaccaa tgcatcggcc tctgcctctg tcattacctt agcttccaca gtactttttc
    18241 gttcattaag cgcattcagt tcttcctgtg ccgatatgag ttcggcggtc acatttacca
    18301 attccgcttc tttatcaccg tactcatatc tcgcctttag aggaagatat atctcataca
    18361 agtcgtcatg tgtaaactga agtacatcta ttctgttcct cgaaaaagta atagccgtat
    18421 ttatatctct ctcctgattc tttacggcat ttagagcttt tgtaaggttt gcaatctgct
    18481 tattatactc atcaatctgc tcccgaaccg gcaggtactc aaaatatctg tatatcatcc
    18541 cattgagttc attctttatt ctgtttattt tatccgcatc gggcggtacc tcattctcgg
    18601 cagcttccag ttcatcttct ttagcttgaa tctccgccat ggtcggtata tcggtatcgg
    18661 gatgtaaggc tatataatca tctctttcct gcatctttgc atccacagcc gattgtgctt
    18721 caagtacggg atttgccatt gctaccagtt gtctttgaag atttttcagg ttttcttttt
    18781 ccgcattcag catgctctcg gcattttcat aatccgcttt tgctattgca taagcaacgg
    18841 gaaattcttc atcgctgtat gaagtatcgg gttttaaaga attgagatat tgttccagtt
    18901 cctgttgttt cacggataat tcatttgtct tcttttccgc caaccttatt tgattctgca
    18961 aatccgcata taaaagttta tttatttcaa gatttgtctt tatacttacg gcttctttaa
    19021 gcaatttgtt aagttccgat atttcttcaa gaataccgct gtaatcgtat gtagattgca
    19081 tcattgctct cttgtaattg atatacatag tggtcagagt attatttgca ttgttaagtt
    19141 catcactcgg agcgtcttcc agacgatata taacggcacc tttttgtaca atctctcctc
    19201 ttcttacatc cacactcttt attacccggg attcatcaaa atatatctga tatgtctgat
    19261 tggcggttac cgttccctga cctcttattc tggaagatat tgtaccgtac ataggatact
    19321 gtacggctac ttccggtaag gaataattca gtatggtgtt ggagaaaaat gtcagcacca
    19381 gcattaccgc taaaaacaca atcgctatat tctttatgat gttccttctt ttagttacat
    19441 ccgtcttcat aaaaaacctc cattaacctt ttatagcact ggaacttgct ataccttcca
    19501 gtatcctgtc ttcgctgtac aggaaaagca gcaacggcaa taccatatat atagtcgcaa
    19561 ctgcaaaagc aagtcctatt tccccggcat ttatcttgga aagaaatacc gaaagaggat
    19621 gcatatcctc gttgttctgt aacattatca agggctgttc taccatattc cagtaatcaa
    19681 tgaataatag caatgctacg gaatatattg cattccttga cataggtaca aataccttac
    19741 tgaatatctg ccactcattg gcgccgtcta tttttgcggc ttccactatc gatgagggta
    19801 ttctctgcat tgttttggtc agtataaata ccgcaaacgg agaaaatatt cccggaagta
    19861 tgatagacca tcgtgtattc aatatgttca tccaatcggc aacaagatag ttgggtacca
    19921 gtgtaacctg atacggcatc agcataagcg ctatataaac aaaaaataac gtattacgga
    19981 acggtcctct gtaccttgca aaactgtagg atgctcccaa tgagattgct atctgaaata
    20041 tgacaatagg tacgacatat atgacggaat tccagaattt aagcagatac tgagggcttt
    20101 ttataagtac ggtaacatac tgaccgaaac tcactttatc cggtattatc ttgagattga
    20161 ctttatcggc aatatatgaa gataccgcgg atgtaccgct gtcggaaaag acttttccgt
    20221 aattggctgt tatttcgctt tttgtcatga aagaattgga gacggtaagt acaacgggca
    20281 ttatgaacat aagagccgta taacccaata ttatcattga tattgcaacg gatattatgc
    20341 gctttatctt atctttgttt cttatacccg ccttattcat ccacactgct ccctaattta
    20401 tcatccatca caaaaagtcc ggcaataata accgtcatta ttatgcatat tataaccgct
    20461 gctgcggaca gtttctgata atccaatgtt ctgaaagtat tgtttatata atgctgcaac
    20521 atgtaaagtc cctcatacgg ataatcaccc gtcagaagat atacctcacg gaaaacttta
    20581 aaggaattga taagcgataa gattgttaca aagaaaatac tggaagaaag ataattcagt
    20641 tttatcctga aaaacattct taccggtccc actccgtcga gcaatgaagc ttcttttaca
    20701 tccacaggga tattggccaa agccgccata aacagcacca tgttgtaccc ggtatttttc
    20761 cataggaaaa gaagaactac caccaacctg gaaaaatccg atttaaacca gtctaccgct
    20821 tcaataccta tcgattccag aaaaacattt atcactccgt tatagtgaaa aactacctgc
    20881 catataagta ttaccgatgc cacaggtact accaagggac ttataagggc ggttctgaat
    20941 atgcttttac cgggaatatt gttttctaag aataaagcaa gcatcaacgg tatcagcact
    21001 gcaagcggca ctgcaattac ggaaaatatc aatgtattct taagcgacaa cataaatgca
    21061 tgattgttaa gaagtgatac ataattgtca aaagcaacaa attctttatt aacgggatta
    21121 tcaataaagg aatagtacag gactataaaa aaaggaataa gaaagaacag taaaaatccc
    21181 gcaatactgg ggatcatgaa cggtacggtg gatgttttat ctcgcttttc gaaacttacg
    21241 tgttttattc ttttctctcc ggtcttatct cttcttttca cgtttctata aatttcctgc
    21301 atttcagtat aactgaaaca attacctttc cttaaataga cgtaagtata ttataaaagt
    21361 tcctttatta ttaatctatt aatattttat taaaaaaata ttcagagtaa aaatgaatgt
    21421 cgattatagg tgtttgtata ataaaaccgc ctgcttttca tctgttattt ttataatacc
    21481 tacagtaatc tcttataaca catcgctcgc atgcgggctt tcttgcatta cagacctctc
    21541 ttccgtgaaa aaccagtctg tgactgaata aagcggattt ttcaggagga attatcttct
    21601 tcaaatcctg ttcaatctta tcgggatcgg tattatttgt aagccctaat ctgcttgaaa
    21661 tcctttttgc atgtgtgtcc acaattatcg caggtttgtt ataaatctcc gcaagtacaa
    21721 catttgccgt ctttctgcct acacccggta acgttgttaa aagattaata tcatcgggaa
    21781 ttattccgtt gaaatcttct ataagttttt tacaacatgc cactatattc cttgctttat
    21841 tacggtaaaa accggtcggg aaaataatgc tctccagttc cttaatatcg gcatttgcaa
    21901 aatcttcagc cgttttgtac tatgcaaaca attcgggagt ggttttgttt actctttcat
    21961 cggtacactg ggcggatagt tgagttgcta caagaagatg aacgggatcg gtataatcca
    22021 gtgtacatct gacgacggga tattcctttt ccaaaagttc tattatttta tctatatccg
    22081 ccatttattt caccgtatcc tgtgtaacgg taataataag cttcttcaaa tcacctgttt
    22141 cgttgcgtct gtagactgtt aactccaggc tttctcccgg taaatatgta ttcttcaatt
    22201 caatgaagtt ttccgcagaa tttacatata catcattaat acttataatc atatctccta
    22261 ctctcaatcc cgaataaaat gcaggtgtac cgtatccgac atatgacaca cggatacctt
    22321 ttacatatat tgttttctga tcttcttcgg aagttattga aaaattccat gcttccgcag
    22381 atatacctat tcccagattg ggtgtagttt cctcattata cgactttact tccaggtttt
    22441 cagaggcttc ttttaccttg ttaatcggaa tagccttaaa gaaaccgttt tgttgttcat
    22501 ctttaataaa aaccgttaaa gcaacaacct gacctttttt atttaccaat gcgccgccgt
    22561 tattacttcc ggtcattaca gtcgaagtgt ttatcatgtg cataagatct ttcggtgttt
    22621 cagcgtcgga taatatcctg tcgttaaatg tgtaagaaag gccggtcaca attcctgcgg
    22681 aaacctgtcc cttgaaagca ttattaccgc ttgcaacggc aacgacggtc tcacctaaat
    22741 acagtttgtc ggaatcgccg tactctataa actgtaacgg tctgtcacaa tctattttta
    22801 aaagagctac gttattggtt ttatctctgc ctatcacctt agcaaaatag agagtgttga
    22861 aatcaaaatc cacaaatacc gtgatggaac agttttcctt aaacttgttg cttttgtcat
    22921 agcacagagg ttctataaca ctgtcagcgg ttatcatata tccgtcattg gaaataatca
    22981 aagcggaggc ttcgttcatg tgaacaccct tttcatccgc atacatgcat ttgatataag
    23041 caattgacgg cattatcttc tgtacggcat cacttatatt ggaatcaact tccagcggag
    23101 ttacctccac tctggatata tccctgtaag tggttataac taaattctct cttttttcac
    23161 tttccggctc aatatccgta tccgcctcct gtgtttcagc aggcaccgga atgtcggtat
    23221 taacaggctc gggtggatct gtcggattac ttaaaaacag caagtcggta tccggctgat
    23281 ttatctttct tacgtagaat actgttaaag cggttgcgca tacggacaat gtaatacaga
    23341 gtatacatac gaacaaagga agaaggattc tggacacaaa acttttcttc cgggttttta
    23401 ttgtctccat gtaatacccg ttatttttta tatcttcaaa taacaattct tcgttttgtt
    23461 cttttttacc gtttctttct gttcttacca ttttgtcctc atagtgatag taccacactt
    23521 taacacttta tttgttaact ttttatgaat tatccgtttg ctcggcattt tccaatgtaa
    23581 agataaattc cgttcctatt ccttttttgc tctttacatc tatcatttct ttatgtcttt
    23641 taataacgct gaacacaatg ggcagtccca accccgtacc tgttccgtct ctgcctctcg
    23701 ctttatcgct tttgtaaaat cttgtccaga tattctccaa atcttcctgc tcaataccta
    23761 ttccgttatc tcttatagaa atatacacct tatctctctt cggttctacc gtaatggtaa
    23821 tattacctcc gtcattggta aacttaactg cattgtgtat taaattgata agcactcttt
    23881 caatatcatc ggaatttgca tagacatagg tcaattcgga atggaaatta acattaataa
    23941 atagtttttt aaacgttatc tcttcctcaa aacgcataat ggactttctt atcatatcat
    24001 ttatgtcaaa ccgattcttt actaaagatg cctcatcatc cgcatccaat ttagtaagaa
    24061 ccagtaagtc tcttaccata ttggtaagcc gctttatttc atctgatact cttttaagat
    24121 aggttttatg cttttcctga ggtatcgttc cgtctaaaat accttctaca aatcctccta
    24181 ttgaagtcat cggggttctc agttcatgag cgacattact ggtaaattcc gctctttgtt
    24241 gctctgtatt ctccagtctc tccatcatgc tgttaaaagt cttggataat tcccctaatt
    24301 cggttctgtc atcggtatac tttgcccgga ctttaagatt gccctgtgaa acagaacgtg
    24361 cagcattcag aaagtcattc aacggtccgg ataccgattt ggcgaaagcc gctaccagga
    24421 gaaccgataa tgcg
//
LOCUS       DAOSOM010000030        24392 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_2067_length_24392_cov_5.943214, whole genome shotgun sequence.
ACCESSION   DAOSOM010000030 DAOSOM010000000
VERSION     DAOSOM010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 24392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24392
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2067_length_24392_cov_5.943214"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="PLF14_07330"
     CDS             complement(<1..948)
                     /locus_tag="PLF14_07330"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOT71138.1"
                     /translation="MRRIKSFRSLALLLAICMIMALFAGCAKKANPLTIWVGVESVDF
                     YTAKMAEYVENYKAKTGEDFPYEIAVQGVDTGSAASKVLDDIDAGADIFTIAHDNLPK
                     LTAGASAIAPVRSQALLTQIKSDNPQAFLDVIDMNVQGTTYTFGIPYIAQSLILMYNT
                     KYLTKDDVKTWEGIWAAAKKHNMQSVSLTGSDGFNNSFLVLAKNETTNRMPTVLYEDG
                     ITENCNFTSDEVVAIMQWGQRFFTGANGAKMPTDSGWEIELRDEISLGVITGAWKFNA
                     AKAALGANLGIATLPSFTLTSEDVKGTNVKAGTVYRSGTF"
     gene            complement(1110..2003)
                     /locus_tag="PLF14_07335"
     CDS             complement(1110..2003)
                     /locus_tag="PLF14_07335"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOT71139.1"
                     /translation="MVIVLFIVMLIGLLLGKMKVLNSESRSAISYLIINVSMPMLIVV
                     SSNLVFDRQTTNEAMWLTIMSVITFFFSILLAQLIFKKAVPRCATVFSNAGYMGLPVL
                     NSILGAKGLFLGAIFQMIFHVFTWTYGISLFTKSGKFKDSLKKMLNPCFIAVIIAVIF
                     MLTGWEIPDTIRTVLKMMGELTTPLSMLLIGATLSQVNFKEIIKVKEIYLVTALRLLV
                     IPAFVLIFLLFPSIPRISVYVVFIINAMPSAAITGILAMKYNNDEKLASSIVAFSTLV
                     SIVTLYLITDVFDVAALLSLM"
     gene            complement(2052..3428)
                     /locus_tag="PLF14_07340"
     CDS             complement(2052..3428)
                     /locus_tag="PLF14_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HOT71140.1"
                     /translation="MLKTRFEDILASNDKKIEQLSEKALASDIISNELYEKYNVFRGL
                     RDNKGVGVITGLTEISEMNGYITNEDGIRVPIEGVMYYRGIDIFDIIKGIMKNNRFGY
                     EECAYLLLCGSLPDRKELEEFVQLLNDFRSVPDSFVRDIILKAPSKDVMNAISRSILT
                     LYSYDNNPDDISVKNVFRQAVQLIAVMPMLAVYSYQAYRHYILGKNLYIRQPKPELST
                     AENFLYMLKGTDRYTQEQAHILDICMILQAEHGGGNNSTFTTHVVTSSGTDTYSCVSA
                     SLGSLKGPRHGGANLKVVHMMDDILKHVKVLSDKNIEAYLEKILAKEAFDKSGLIYGL
                     GHAVYSVSDPRALILRDQLVKYMSDKKKDRLYTLYDKLQYIAPEVIRKKRNVTGNIAI
                     NVDYYSGYLYRQLDIPEELYTPIFAISRILGWSAHRIEELANNGKIIRPAYIAVADRS
                     EYIDIDER"
     gene            3660..4841
                     /locus_tag="PLF14_07345"
     CDS             3660..4841
                     /locus_tag="PLF14_07345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT71141.1"
                     /translation="MRIGQFTDSFLPIVDGVGRVVYGYADALSRRGHECYVITPISDA
                     GFRGKYPFDIVDFYGVTVPEYTQYKMGIPAWDIHYHERIKTIKLDIIHVHSPFRSGFE
                     GLRLSKKMKIPLVGTFHSKYYEDFLQALKVQTLAEAGAMMVGNFYKKCDEVWAVSDIS
                     AEALKSYGYKGDIRIMRNGTEVRKPDRKNVNYVKTLFNLDDVPVILFVGQMNWKKNIQ
                     KVLEAVSLLDRQGYKTKLILAGQGPHTEEIKRKITELNISDSVEMTGHISDVNILDGL
                     YGLAEILAFPSLYDNSPMVVREAAAMMTPSLLVRSSSASEGITDGYNGYLCEDNSKDI
                     ASVLKRVLFEEKNGIRQVGRNAQMTIPVSWDTVIEDVEKCYFDLIRMKKNKRAGRKEY
                     V"
     gene            4855..6567
                     /locus_tag="PLF14_07350"
     CDS             4855..6567
                     /locus_tag="PLF14_07350"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT71142.1"
                     /translation="MKPNIKTPWLTSYKDLPFYLDYPDISMFDMMERAAKSTPNLYAY
                     DFLGVKCTFKNFVKKVRQCAKALTAIGIKQGDRVTVCLPNCPQALFMFYGLNLIGALG
                     NMIHPLSSEGEIEFFLNDSKSKAAITLEQFYGKFAAIKSKVHPEILIISSIKDELNIP
                     LSIGYELTQGRKIKKVPKDAKILKYKDFMKKGKAFKGSYEVERKGNDPAVILYSGGTT
                     GTTKGILLSNLNFNALAMQTMIMGNCNKPGNTMLSIMPVFHGFGLGICIHTALCASCT
                     CILVPRFNAASYAKLLKKKQPNYIAGVPTLFEALLRNPYMDNVNLSCLEGVFSGGDSL
                     SIELKRKLDKFLKEHGCNVQVREGYGTTECVTASCLTPKDFYKEGSIGIPYPDTYYKI
                     VKVGTTEEVPYNEEGEICIHGPSVMIGYCNNEKETAETLRKHDDGYIWLHTGDLGKMD
                     EDGFVYFSQRIKRMIITSGYNVYPSRLENILDAHEAVSMSCVIGVKDPYKIQKVKAFV
                     VLKAGFEPNDITKQNIFEYCKKHIAKYALPYDIEFREDLPKTLVGKVAYRELEKEEMN
                     KMGA"
     gene            complement(6612..7940)
                     /locus_tag="PLF14_07355"
     CDS             complement(6612..7940)
                     /locus_tag="PLF14_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71143.1"
                     /translation="MITITRMEPIDKGLVFEYKCSSEYELVMTVKPLTTSMPLIQKKL
                     DKSETKVTVNNLINNLDYFVSLTSVDNSGRIKEPSCTRLFTPYPNKRKFKTVNYIHKD
                     DYYYYPSGKSTCSPSILKLDENTYICSHDVFWQYTAQNLTLFFKSKDKGETWEYLSQL
                     SPCFWGSLFLINGIVYMYGISTEYGDIMLHKSYDKGATWPESVCLFAKCQDDIKGFHK
                     APMPAIISDGRLWLNVEYGTWKHKFAIHALSCGIDDDIMNPANWTITKPLYITKNDEG
                     IIAKEDPVCIEGNLVQGFDGKLYNILRCNLIQNISAVLCEVNKQDISAPLKFIKAVDL
                     PVSHSKFVVRTHSDGYHYAMGNRIDERCKNIRQILSVYRSKNLIDWEFCKDIADLLGT
                     ADRSKAALQYPDWFIEEKDIVYVNRTAINKPYNFHNANNITFHRYKNFFA"
     gene            8147..9574
                     /locus_tag="PLF14_07360"
     CDS             8147..9574
                     /locus_tag="PLF14_07360"
                     /inference="COORDINATES: protein motif:HMM:NF015573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HOT71144.1"
                     /translation="MPTGMNFWDTGMWSFIITLTILLTCMMVANFLRRVIKPLRRSLI
                     PSSVLGGFLALFADFIFKKITGNSMFNPVMLEALTYHGLGLGFAAVALKTTERKIVGD
                     SKKDIMNYGLTTVSAYVLQGAIGIAIMLILGLFLSDTFPAAGLLLPMGYGQGPGQAYN
                     WGHTYEVLWGFEHGTSFGLTLAATGFISASVGGIFYLERLKRKGLVKNTFEDEIVASE
                     EDEENKPAKGEIPLSESMDKLTVQVALVLISYALAYLFMWGINKIIEAGILGTFGTNT
                     LQPLIWGFNFLISTVFAVLVKTFLRFLKKKKIMKREYTSNFMQNRISGFMFDIMVVAS
                     IAAIKLSAFQHRSFIIPIIALSVAGCVFTYIQCDFIAKRLFKGYEHESFLALYGMLTG
                     TASTGIILLREIDPYFSTPAATNLVYQQLYAILFGFPVLLLLGFAPQSNTNLWITFIV
                     LIVLLAVYTVLLFRDSIFKKKKKTA"
     gene            9698..11164
                     /locus_tag="PLF14_07365"
     CDS             9698..11164
                     /locus_tag="PLF14_07365"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HOT71145.1"
                     /translation="MENDKIFNRNKWFYSLGGIGRDMAYQLFSAYLLTYVMFTKTLTN
                     AQFAAISIIMVICRIFDALNDPVMGGIIENTRMKWGKFKPWIVIGALTNAVVMVIYFS
                     SPLEGWPFVIFFACMYLLFDITFTMNDIGYWAMLPSLAKDQKQRATLTSMANLFASIG
                     AIIAFATIPILTNGPNAIGGNSITGYMWVSIIIAILFVAFQLMTAILVKEAPVSYRSE
                     GEKIGLKAMFKVVFKNDQLLWTSLVMLLYNLGSSLINVFGIIYVYMYFGYDGINITMF
                     VGAYALGNLLINIVYPQLVKVMTRKQIATTGLIGVIIGYGFFFIIGVLIPMNYLLLCV
                     SGFILSFGQAMIYMVTTINLTNCIEYNEYKTGNRDEAIIFSIRPFMAKMSSALQQGIV
                     GAAYLALGITAITNGISEAERLSNMGLISAEEKSTMIEQALALGTDSMKMNFRIFIVV
                     VPIILISLAYLVMMKKNKIDEETYDRMMLEISQRKQAE"
     gene            11378..12805
                     /locus_tag="PLF14_07370"
     CDS             11378..12805
                     /locus_tag="PLF14_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005348908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOT71146.1"
                     /translation="MQKDTNPVLQFSDKSNLLEQSAYKYQLQDRETPNLYRYLYDYKT
                     VPKVPFNHRSVPMNMPKEIWITDTTFRDGQQSTSPFSVEQIVHLFKLMHKLGGPKGLI
                     RQSEFFVYTEKDRKAVEQCMSLGYKFPEVTSWIRANEKDFDIVKSLGLKETGILVSCS
                     DYHIYNKMGLTRKQAMDKYLGIVKSVLDKGIKPRCHFEDITRADFYGFVVPLAYELTK
                     LSEETKIPIKIRACDTMGFGVSYPGAALPRSVQGIIYGIYHYGGVKSELIEWHGHNDF
                     YKVVTNASTAWLYGASGVNCSLLGIGERTGNCPLEAMVIEYASLKGTTDGMSIPVITE
                     IAEFFEEEIGYEIPPRTPFVGRNFNVTRAGIHADGMLKDVEIYSIFDTEALLNRPASV
                     AVDSHSGLAGVAHWINSYFRIKDKEIAVKKTDSVVAKVKELVDKEYADGRNTVMGDGE
                     LENILKSVDYDEYEKLSRKHARKRK"
     gene            12960..13904
                     /gene="arcC"
                     /locus_tag="PLF14_07375"
     CDS             12960..13904
                     /gene="arcC"
                     /locus_tag="PLF14_07375"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018167453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOT71147.1"
                     /translation="MIKRVVVALGGNAILTKDPTAEGQIQALRETSEKLVELIKHDYE
                     LIITHGNGPQVGNLLLQQEAAHSKSNPAMPVDTAVAMTQGSIGYWLQNTLDEAICKSG
                     MEKSVATILTQVVVAEDDPAFKNPTKPIGPFFSKEQAEKRAEESGDTYMEDAGRGYRK
                     VVPSPKPVSVIEADTIRTLVENGVITISAGGGGIPVVKRGSKYVGVEAVIDKDFAAAK
                     LAEIVKADYLVILTGVENAYINFNKPDQKKLEHVTVAEMKQYIAEKQFAAGSMLPKIQ
                     AAISFVESVEDGKAFITSLDNVNAIAGSGSATVITKDI"
     gene            14121..15221
                     /locus_tag="PLF14_07380"
     CDS             14121..15221
                     /locus_tag="PLF14_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HOT71148.1"
                     /translation="MKKLICVLLVVVFALSLVACGDVGKQGKKDFIGIILPTKEESRW
                     LNDQKYFEEYFKGMNYEILFSQNNSATEKTNVETFISKGAKVIVLCAYDAAAAASAVN
                     EASKEGITVISYDRLIMGTDKLDYYVTFDSCSVGKAQADYLVEKAGSEKGINLYMYSG
                     ALTDNNSVLFFEGAWKVLQPKIADGTFVVRNCEKAIQFKDKNNLTREEMVSIMQTIDS
                     EWNMERCKSLAEANLTAAGDKGKGKVYVLGPADDDCCRALSDAFLADPKVTELKITGA
                     DGVEASVQYIIDGKQSMTVYKDTQALVKATKQIIEALQKGEKPKTDTVYNNDAKDVPT
                     IQADVVTVTADNIAEIFFESGVYDGSKYTGWK"
     gene            15299..16837
                     /gene="gguA"
                     /locus_tag="PLF14_07385"
     CDS             15299..16837
                     /gene="gguA"
                     /locus_tag="PLF14_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOT71149.1"
                     /translation="MNDYILEMHGISKSFPGVKALIDVNFSVKRGEIHCLVGENGAGK
                     TTLMKVLSGIHPYGSYEGDIVLNGQIMKFNTIRESENTGIAIINQELALIPDLTVAEN
                     IFFGHEIMKRGLVDWNETYKQADIYLKKVGLNINPRSLMRDHRVGTQQLVEIAKALSK
                     NAKLMILDEPTSSLNEEDSANLLRLLKELQKQGVTIIMISHRLKEVVSIAETITILRD
                     GATVYTMRADEEEITENKIIKYMVGREMDNIYPHRESCVTDEVLFEVKDWCAYNAKSG
                     QTVLKNINLKVHKGEIIGMAGLMGAGRTELALSIFGNTPEYKITTGELYLEGNRVRFS
                     SPKDAIKKGVAYATEDRKKNGLILIQDIKFNTSFANLDRIRNRGVINRNEEVVQANKL
                     KESINIKAPSVQQILRNLSGGNQQKVVLAKWIFATPKLMILDEPTRGIDVGAKFEIYT
                     LMNEMVKQGMSIIMISSELLEVIGMSDRIYVMHEGKITGELSKSEATEQKIMHFATNT
                     EELS"
     gene            16834..18024
                     /gene="gguB"
                     /locus_tag="PLF14_07390"
     CDS             16834..18024
                     /gene="gguB"
                     /locus_tag="PLF14_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOT71150.1"
                     /translation="MSENKKTGREYGKTLVTNLRNYLMYIALAVIFIVFQILSNGSFL
                     TARNITNLINQTGYIAVMAVGMTLILIIRQIDLSVGFVAGFLGAAAARLLKAGVAVLP
                     TILIVLAMGVMIGLVQGLIIGKVGVPAFITTLAFEFGFRGLLTLITEATGTIPIQSDV
                     FYELSNGFIPELFKIGDLSGSCLIAGAISMALIVFSEIRKRKELKRYKFEDMPVLLFI
                     LKLLFFVALIGTLTVVLAGYRGISWTIVIVVIVIAIYSFVMNKTKVGRYIYGMGGNPE
                     AAQLSGVDIKRILIGSFASMGLMAALGGILYTSRLGSATPTAGMGFELDAIASCYIAG
                     VSTSGGVGSVANSVIGAFVIMSLTNGLNLIGVGISYQYLIKGIIFILAVALDVRSRGK
                     KALG"
     gene            18152..18228
                     /locus_tag="PLF14_07395"
     tRNA            18152..18228
                     /locus_tag="PLF14_07395"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18186..18188,aa:His,seq:gtg)
     gene            18242..18316
                     /locus_tag="PLF14_07400"
     tRNA            18242..18316
                     /locus_tag="PLF14_07400"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18274..18276,aa:Gln,seq:ttg)
     gene            18324..18399
                     /locus_tag="PLF14_07405"
     tRNA            18324..18399
                     /locus_tag="PLF14_07405"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18357..18359,aa:Lys,seq:ttt)
     gene            18515..19330
                     /locus_tag="PLF14_07410"
     CDS             18515..19330
                     /locus_tag="PLF14_07410"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOT71151.1"
                     /translation="MKVKRNHNVTNRNPEAMEVTVEERKEKILDMLNRDGRVKVNELS
                     KLFDVSEVTIRLDLSDLEESGLLSRVYGGAVSSYKSYYNMSFKQRSAANSNEKKAIAS
                     RVSQMVAENDTLMMNSGTTTLITYMALPKNMHLTVITNSVAIAQEASGNDNHKILLLG
                     GNVNTDYQFTWGEDSLNQLSAYHADKAILSVDGISVKSGLTTYYHQERHITSQMISNS
                     DIAIVTADYTKIGRAALSKITFADRADCIITNLNASAEEIRMLSGKGIKVHLV"
     gene            19341..20840
                     /locus_tag="PLF14_07415"
     CDS             19341..20840
                     /locus_tag="PLF14_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71152.1"
                     /translation="MKTYIKAGWRENSITPTKRASLAGQFYERISEYVETDITVTALA
                     VSSEYDHFVICSCDLLYVQESLMDKVRQKIEGRCEGLDPMKVILNATHTHTSIDYGNP
                     QSKYGSALDVLKKYVPEDFTYTEKVRDDSIIQPEEAEIFLADSIADAVINAWNNRKKA
                     YFSNAFGRAPVGMCRRAVYKDGTALMWGDTQRGDFIHLEGGNDSGIELIYFYDEKEHL
                     TGIVANIACPSQILEHRSFISSDYWGKVKILLRKQLGPDIFLLALCGAAGDQCPRDLI
                     RWVPPITPIDDPNITRKDTVKRVADPSMFDIEGSWKAAKRIANEIIDVYSEAKEHVTV
                     NAVIKHECIDLQLPLRKATEKEYEYALFKIKEFVKNKESTTYDFNDKAKMHVHAGTVA
                     RYMYQQKEDKCNIEMHVIRMGDIAIATNPFELFLDYGNIMKARSRASQTLIVQLACGC
                     GNYLPTKKAEQHGHYSAYISSGITGHAGGRKLTEVTLETIDKLWEEEDK"
     gene            20837..22249
                     /locus_tag="PLF14_07420"
     CDS             20837..22249
                     /locus_tag="PLF14_07420"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOT71153.1"
                     /translation="MNLLGLDFGTTSLKAAVFNKEGQCLFVHEEAYELIHNDGFIEFD
                     AKEYLNILKRAIKAATDKFSIYAMAIDTQCETIILADQSGNPLCNAVVWLDDRAKQQA
                     LKIKEDFGNRQVYEVTGQCDISGIWPACKLLWFKQNRPDIWKKTGKIFLLEDYLLYNL
                     TGKFVTQKTLQSSSLYFDIVNGCWWKTMLDYIGISEDMLPALYDSGAYVGDYNGIHIA
                     TSAMDQAAGAIGAGVYKEGVISEMTGTAMAIFSPTDNMPLYHEDIIVPCHYNATTKYA
                     VMPWMPTAGMALKWFKDCFMYDMTYAQLDMLAKDAPPGSDGLMFFPHLCGSIHPILNP
                     HAKGSFYGIGLAHKQEHFVRAILESIAYMLKSCIASLECDLDAIHSIGGSAKSDIWCR
                     IKADVTQKKIITLKEREPACKGSAILAGLATGVYSSVGQACEKTLATDRVILPERDVY
                     AGLYEEYMKINEKIYGGECK"
     gene            22246..23595
                     /locus_tag="PLF14_07425"
     CDS             22246..23595
                     /locus_tag="PLF14_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71154.1"
                     /translation="MSKVAFLGFGEVNTPRELIVKKCKKAENALKKEGLDLISIYPIT
                     DDSEREDVLKAIDKLSKASFGTIVLCVAGWIPSHAIIDIIERFRHIPMVLWGLCGWME
                     GDRLVTTADQAATSAIRKVMEDLGYSFKYVYDIVGKPMNTQKVADFCKASEAAAKLRS
                     SRAGMMGYRDMNLYGTMFDAIRLKKYIGTDIESFDMLEVNRRAEKVTKEAIDNVIEQD
                     MSKWVFLKPCDRETKEKAAKYYLAISDICKERKYEAVSLKDVDGMKKFLFYPPAPIFM
                     LLSDVDRLSTIPENDSYGMVTQLMVKYLTGQCGAYLEFYEYMEDCVIAGVPDYVPKEI
                     IDGDTLVMPAAFGLLSQGILNVSKVKQGKVTLYRLAESKNGFCMHIVQGRAESPGKWE
                     EAGWTQPAPQLPGLKVYMDDVEAFTSKVMSQHYIIAYEDNADKMIDLCKLLNIEVIK"
     gene            23592..24185
                     /locus_tag="PLF14_07430"
     CDS             23592..24185
                     /locus_tag="PLF14_07430"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOT71155.1"
                     /translation="MSKDVLQAAYFSLDTEKKEISRLKDYLDEQCFLKAVDALANCKS
                     IMTVASGSSGIAAKKFAHSLCCINKHAMFMPPSEAVHGGLGALDEQDVMVMVSRGGKT
                     VELLPIITGCKKKKAMLIGVTENDQSILAKNSDILLKLSIERESDPLGLMATSSYVAT
                     IALFDALLASLIVKTGFTAEMFGVIHPGGAVGELLNK"
     gene            24194..>24392
                     /locus_tag="PLF14_07435"
     CDS             24194..>24392
                     /locus_tag="PLF14_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOT71156.1"
                     /translation="MYKGLKISAPFFEIGPKAYLYGEKMLALAKVIDKVATKYDVDVI
                     VTPQYTDISLLAHNTERISVFA"
BASE COUNT         7385 a         4528 c         5634 g         6845 t
ORIGIN      
        1 aaaggtgccg cttctgtata cagtacccgc cttgacattt gttcccttga catcctccga
       61 cgtcagtgta aaggaaggaa gtgtggctat tccgagattt gctcccagag cagcttttgc
      121 cgcattgaat ttccatgcac cggttattac gcctaaagaa atctcgtctc tcaactctat
      181 ttcccatccc gagtcggtgg gcatcttggc gccgttagca ccggtaaaga accgttggcc
      241 ccactgcatt attgccacta cttcatcgga agtaaaattg cagttttcgg taatgccgtc
      301 ttcatacaat acggtaggca ttctatttgt cgtctcgttt ttggcaagaa ccaaaaatga
      361 gttattgaat ccgtccgatc ccgtaagaga gaccgactgc atattatgct ttttcgctgc
      421 tgcccatatt ccttcccatg tttttacatc gtcttttgtc aggtattttg tgttatacat
      481 cagaataagc gactgtgcga tatacggaat accgaaggta tacgttgtgc cttgtacatt
      541 catatcgata acatccagga atgcctgagg attatcggat tttatctgag tcaataaggc
      601 ctggcttctg accggagcta ttgccgatgc acctgccgta agcttgggca gattgtcatg
      661 tgcaatggta aagatatccg cgccggcatc tatgtcatcc agcacctttg atgctgccga
      721 accggtatcc actccctgta ccgcaatctc gtaagggaaa tcctcaccgg ttttggcttt
      781 gtagttttcc acatactccg ccatcttggc agtgtaaaaa tctaccgatt ccacacccac
      841 ccatatagtg agagggttgg ccttttttgc acatccggca aataatgcca taatcataca
      901 tatggccaaa agcaatgcca atgacctgaa cgattttatt cttctcatat tcactcctcc
      961 tgtaaagata caaatctaat tgattttatg ttctattaac atacaaatta tacatccatt
     1021 gaatagtaag tgtcaaatat tgttgcggtt ttaaatttac aattatttga agagaaattt
     1081 atataaaaaa gagtaaaata aacggcattt cacattaaag aaagcagggc cgccacatca
     1141 aaaacatcgg taatgaggta cagggtgact atggatacca gtgtggaaaa agccacgatt
     1201 gaagaagcaa gtttctcgtc attattgtat ttcatggcca atattcccgt tattgccgcc
     1261 gacggcatgg catttattat aaatacaaca taaacggata ttctcggtat tgagggaaaa
     1321 agaaggaata tcagtacgaa tgcgggtatt accagcaggc gcaaagccgt tacaaggtat
     1381 atttctttga cttttattat ttccttaaag ttgacctgtg aaagtgtagc tcctatgaga
     1441 agcatggaaa gcggggtggt caactcaccc atcattttca aaaccgtccg tatagtatcg
     1501 ggtatttccc aacctgtcag catgaaaata acggctatta tcaccgctat aaagcaggga
     1561 ttaagcattt ttttaagaga atccttgaat ttgcccgatt tagtgaacag tgagataccg
     1621 tatgtccatg tgaaaacatg aaaaatcatc tgaaatattg cacctaagaa taagcctttt
     1681 gcacctaaaa tggagttaag tacgggcaaa cccatgtaac ctgcattgga aaatacggta
     1741 gcacatctcg gtacggcttt tttaaatatc agttgagcca gcagaatgga aaagaagaaa
     1801 gttataaccg acattatggt caaccacatt gcttcgttcg tagtctgccg gtcaaacacc
     1861 agattgctgg atacgactat gagcatgggc atggaaacat ttatgataag gtaagaaata
     1921 gcacttcttg attcggaatt cagtaccttc atctttccta aaagcaaccc tattaacata
     1981 acaataaaaa gtacaataac cataatatcg gtattatata tcaaaatatt ttacatgcaa
     2041 catcaataag cttatctctc atcaatatcg atgtattcgc tgcggtctgc aaccgctata
     2101 tatgcgggac gtattatctt accgttgttt gccaactctt ctatcctgtg agcagaccag
     2161 cccaaaattc tggagattgc aaaaatagga gtatataatt cctcgggaat atccagttgt
     2221 ctgtaaagat atccggaata atagtcgaca tttatcgcaa tatttcccgt tacattcctt
     2281 tttttccgta ttacttcggg agctatgtac tgtagcttat cgtacagggt gtataatctg
     2341 tctttctttt tgtcggacat gtattttacc agctgatcac gtaaaatcag tgccctggga
     2401 tcggataccg agtagaccgc atgccccagt ccgtaaatga gaccggattt atcaaatgct
     2461 tctttggcaa gaattttttc cagatatgct tcaatattct tatccgaaag tactttgaca
     2521 tgttttaata tgtcatccat catgtgtact actttcagat tggcacctcc gtgacgggga
     2581 cctttcagtg aaccgagcga agcggataca caggaataag tatcggtacc gctggatgtt
     2641 accacatgag tggtgaatgt ggagttgtta cctccgccgt gctccgcctg cagtatcata
     2701 catatatcca gaatgtgcgc ctgttcctga gtgtacctgt ccgtaccctt aagcatgtac
     2761 agaaagttct ctgcagtgga aagttccggt ttgggttgtc ttatgtagag attctttccg
     2821 agtatatagt ggcgatatgc ctgataactg tatacggcca gcatgggcat taccgctatc
     2881 aattgtacgg cttgtctgaa tacattcttt acggatatat cgtctggatt attgtcatac
     2941 gagtaaaggg taagtatgct tctggaaatg gcattcataa catccttgga gggagctttt
     3001 agaataatat ccctgacaaa actgtcgggt accgaacgaa aatcgttaag cagttgaaca
     3061 aattcctcca attccttcct gtcgggtaga gatccgcata acaacagata agcacactct
     3121 tcatatccga atcggttatt cttcataatg cctttgatta tgtcgaatat atcaatacct
     3181 ctgtagtaca ttacgccttc aatgggaaca cgtataccgt cttcatttgt tatatacccg
     3241 ttcatttcgg atatctcggt aagtccggtt attacaccta ctcccttatt gtcacgtaag
     3301 cctctgaaaa cattgtattt ttcatataat tcattactaa taatgtcact tgccaatgct
     3361 ttctcgctga gttgctctat ttttttgtcg tttgaggcta atatatcctc gaatctggtt
     3421 tttaacaatg attgtccttc cttttttcgt atgtaataat aacataaatc atacttctaa
     3481 taaagttatt tataacatct tcgattgccc gacactcttt tttgctattc tttatgtctc
     3541 aggataattg actcggttat gataaaggaa tataattgaa cttgtggata ctttgccgtc
     3601 aggttatatg gggttaacgc tgatttgcat attaagagta ttcggacagg aacttaaaaa
     3661 tgaggatagg acagtttacc gattcatttc tgccgatagt tgacggagta gggcgggtcg
     3721 tttacggcta tgcggatgca ttaagcagga gaggacacga gtgttatgtt attacaccca
     3781 tttccgatgc cggtttccga ggcaagtatc cgtttgatat tgtggacttt tacggagtta
     3841 cggtacctga atatacacag tataagatgg gtatacccgc atgggatatc cattatcatg
     3901 agagaattaa gacaataaaa ttggatataa ttcatgtgca cagtcccttt cgttcgggtt
     3961 ttgaagggtt acgtttgtca aaaaaaatga agataccgtt agtcggaacc tttcattcca
     4021 aatattatga agattttctg caggctttga aagtacaaac tctggcagaa gccggagcga
     4081 tgatggtggg aaatttttat aaaaaatgtg atgaagtctg ggcggtaagc gatatttcgg
     4141 ctgaagcctt gaaaagttac ggatataaag gtgatatcag gataatgcga aacggtacgg
     4201 aagtgcgaaa acccgacaga aagaatgtca actatgtgaa gacacttttc aatttagatg
     4261 atgtgcctgt aattcttttt gtggggcaga tgaactggaa gaagaatata caaaaagtac
     4321 tggaagcggt cagcctgctt gataggcagg gatacaaaac aaaattgatt ttagcggggc
     4381 agggacctca tactgaagaa ataaagagaa agataacgga actgaatata tccgacagtg
     4441 tggaaatgac agggcatatt tccgatgtca acatactgga cggtttgtac ggattagcgg
     4501 aaatattggc ttttccgtca ttgtatgata acagccccat ggtggtaaga gaagcggcag
     4561 caatgatgac accttcgtta ttggtgcgca gcagcagtgc atcggaaggc attacggacg
     4621 gatataacgg atatttatgc gaagataatt caaaagacat agcaagcgtt ctgaaaaggg
     4681 tgttgtttga agaaaagaac gggataagac aggttggcag gaatgcacag atgacaatac
     4741 ctgtttcctg ggacaccgta atagaggacg ttgaaaaatg ttattttgat cttattcgga
     4801 tgaagaagaa caagagggca ggcagaaagg aatatgtata ggaaaggtga taacatgaaa
     4861 ccgaatatta aaactccgtg gcttacaagc tataaggatt tgccgtttta tctggattat
     4921 ccggacattt ccatgtttga tatgatggaa agagcggcaa aaagcactcc aaatctttat
     4981 gcatatgatt ttctgggagt aaaatgcaca tttaagaact ttgtgaaaaa agtccggcag
     5041 tgtgcaaaag cattaaccgc tatcgggatt aagcaaggtg acagagtaac ggtctgtctt
     5101 cccaactgtc cgcaggcatt gtttatgttt tacggattga atctgatagg agcattgggc
     5161 aatatgattc atcctctgtc ttccgaaggg gagatagaat tctttcttaa cgattcaaaa
     5221 agcaaggctg caataaccct tgagcagttt tacggtaaat ttgctgcgat aaaatctaaa
     5281 gtgcatccgg aaatactgat aatatcctcc ataaaggatg agttaaatat tcctctttca
     5341 atcggatatg aacttactca gggcagaaag ataaaaaaag tgcctaaaga tgcaaaaata
     5401 cttaagtata aagacttcat gaaaaaaggc aaggctttca aaggcagtta tgaggtggaa
     5461 cgaaagggta atgatcctgc ggtaatactg tacagcgggg gaactacggg tactaccaag
     5521 gggatactgc tgtccaacct caattttaat gctcttgcca tgcagacaat gataatggga
     5581 aattgcaata agcccggtaa taccatgctt tccattatgc cggtttttca cggattcggg
     5641 ttgggtatat gtatacatac cgcgctgtgt gcttcctgta catgcatact ggttccccgc
     5701 ttcaatgcgg cttcctatgc aaaactgctg aaaaagaagc aaccgaatta tattgcgggt
     5761 gtacccactc tgtttgaagc tctcttaaga aatccgtaca tggataatgt aaatttaagc
     5821 tgtcttgaag gagtattctc cggcggagat tctttatcaa tcgagcttaa gagaaagttg
     5881 gacaagtttt tgaaagaaca cggctgcaat gttcaggtgc gggaaggata cggtaccacc
     5941 gagtgtgtta ccgcaagttg tcttactccg aaggacttct ataaagaggg aagtatagga
     6001 atcccttatc ccgacacata ttataagata gtcaaagtgg ggacaacaga ggaagttccg
     6061 tacaatgaag aaggagagat atgtattcac ggtccgtcgg taatgatagg ttactgtaat
     6121 aatgaaaaag aaaccgccga aacactgaga aaacacgatg acggttatat atggcttcat
     6181 accggtgatt tgggcaagat ggatgaagac ggattcgtgt atttttctca aagaataaag
     6241 agaatgatta ttacttcggg atataatgtt tatccgtccc ggctggagaa catacttgat
     6301 gcccatgaag cagtatccat gtcttgtgta ataggtgtca aggatccgta caaaatccag
     6361 aaagtaaagg catttgtggt attgaaagcg ggatttgaac cgaatgatat tacaaaacaa
     6421 aacatatttg aatactgtaa gaagcatata gcaaaatatg cccttccgta tgatattgag
     6481 ttcagagagg atcttcccaa aacacttgtg ggaaaagtcg cttacaggga actggaaaaa
     6541 gaagagatga acaaaatggg agcctaagac tcctttttgt ttgttgtcgg ttgcaaaact
     6601 tctttgtcgg attatgcaaa gaagttttta tatctgtgga aagttatgtt atttgcgtta
     6661 tggaagttat acggtttatt gattgcggtt ctgtttacat aaactatgtc tttttcttca
     6721 ataaaccagt cgggatattg caatgcggct ttacttctgt ccgctgtgcc taaaagatcg
     6781 gcaatgtcct tacagaattc ccagtctata agatttttcg agcgatatac gctgagtatt
     6841 tgtctgatgt ttttgcagcg ctcgtctatt ctgtttccca ttgcgtaatg atatccgtcg
     6901 ctatgggttc tgacaacaaa tttactgtgc gaaacgggaa gatccaccgc ttttataaac
     6961 ttcaacggtg ccgatatgtc ctgcttattc acttcacata agacggcgga tatgttttgt
     7021 attaaattac atcggagaat gttatacagt ttaccgtcaa aaccctgcac aagattgccc
     7081 tctatgcata cgggatcttc ctttgcaatt attccctcat cattttttgt aatgtataaa
     7141 ggttttgtga ttgtccagtt ggccgggttc ataatgtcgt cgtcaatacc gcaggaaagg
     7201 gcatgtattg caaatttgtg tttccaggtt ccgtattcaa cattcaacca cagtctgccg
     7261 tctgaaataa tcgcaggcat gggtgctttg tgaaatcctt tgatatcatc ttgacacttt
     7321 gcaaacagac ataccgattc cggccaggtt gcgcctttat cataagactt atgcaacatt
     7381 atatccccgt attctgtgct tattccgtac atatatacaa ttccgtttat caaaaagaga
     7441 ctgccccaga agcagggaga caactggctt aagtactccc aagtttctcc tttgtctttt
     7501 gacttaaaaa acagtgtgag attctgagcg gtatattgcc agaagacatc gtgtgaacag
     7561 atataggtgt tttcatccag tttcagtata ctcggcgaac aggtagattt acctgaagga
     7621 tagtaatagt agtcatcttt atgtatgtag tttaccgttt tgaactttct tttattcgga
     7681 taaggggtaa aaagcctggt gcacgacggt tctttgattc ttcccgaatt gtcaactgaa
     7741 gtgaggctga caaagtagtc caggttgttt atgagattat taacggttac ctttgtttcg
     7801 gatttgtcca gtttcttttg tatcaagggc atacttgtcg tcaacggttt tacggtcatt
     7861 acaagctcat attccgaact gcatttgtat tcaaatacca gtcctttatc tatcggctcc
     7921 attctggtta ttgtaatcat gtcatcctcc gctaatgctt attttaatct taccaaacaa
     7981 atttgcctta ttccaatata taacaggtag gaaagagcaa aaaatattga aaaagagaaa
     8041 aacaaccgag catttaacca atattttata ttgcaatcag cagttcatgt gttatactga
     8101 aataagttat gactagctca taaaaacttt caaaggagag tatattatgc ctacaggaat
     8161 gaacttttgg gataccggaa tgtggtcgtt tataataaca ctgacgattt tgcttacctg
     8221 tatgatggta gcaaattttt taagaagagt tataaagccg cttagaagat cgttaatacc
     8281 cagctcggtg ttgggaggtt ttctcgcact gtttgccgat ttcatattta agaaaattac
     8341 aggtaattcc atgttcaatc ctgtaatgct ggaagcattg acttatcacg gtttgggctt
     8401 gggatttgcg gccgtggcat taaaaaccac cgaacgtaaa attgtcggtg acagtaaaaa
     8461 agatataatg aattacggct tgacaacagt atccgcttat gtgcttcagg gagcaatcgg
     8521 tattgccatt atgttgattt taggattatt cttgtccgac acattcccgg ctgcagggct
     8581 tcttttacct atgggatacg ggcagggacc gggtcaggca tataactggg gacataccta
     8641 tgaggtcctg tggggatttg agcacggtac aagtttcggt cttactcttg ccgctaccgg
     8701 ctttatatcg gccagtgtag gaggtatttt ttatctggaa agattaaaaa ggaaagggtt
     8761 ggtaaaaaac acgtttgaag atgagatagt ggcatcggaa gaagatgaag aaaataaacc
     8821 ggctaaagga gagataccgt tatccgaatc catggataag ctcacagtgc aggttgcttt
     8881 agttcttatt tcctatgctc ttgcttatct tttcatgtgg ggaatcaaca aaataataga
     8941 agccggcata ttaggtactt tcggtacgaa tacattgcaa cctcttatat ggggatttaa
     9001 cttcctgatt tccactgtat ttgccgtcct tgttaagact ttcctcagat ttctcaaaaa
     9061 gaagaaaata atgaagaggg aatatacatc caatttcatg cagaacagga tatcggggtt
     9121 tatgttcgat ataatggtgg tggcttctat agccgcaata aagctttcag ctttccagca
     9181 tcgttcattt ataataccca tcatcgcatt atccgtagca gggtgtgtgt ttacttacat
     9241 acagtgtgat tttatagcta aaagactgtt taaaggttat gagcatgagt ctttccttgc
     9301 tttgtacgga atgctcacag gaacggcaag taccggaata atccttctca gagagattga
     9361 tccttacttt tcgactcctg ccgccaccaa tcttgtgtac cagcagttgt acgctattct
     9421 gttcggtttt ccagttcttt tactccttgg atttgccccg cagagcaata caaacttatg
     9481 gataacattt atagtcctta tagtattgct ggcagtatat actgtactgc ttttcagaga
     9541 cagcattttt aaaaagaaga aaaagactgc ttagtaaacc tgagcagtga aaatgaataa
     9601 gcggggaata aaaacagttc tgttaaaacg gactccggat ggtatataat aaaatggtaa
     9661 ggattttaaa tacaatttga tatagaagga gaggggtatg gagaacgata agatatttaa
     9721 tcgaaacaag tggttttact ctttaggcgg tatagggcgt gatatggcat atcagctttt
     9781 ctcggcgtat ttgctgactt atgtcatgtt tacaaagacg ttgaccaatg cccaattcgc
     9841 cgcaatatcg attattatgg taatatgccg tatattcgat gctttaaatg atcctgttat
     9901 gggaggtatt attgagaata ccagaatgaa atggggaaaa ttcaagccat ggattgtaat
     9961 aggagcattg acaaatgccg tagtcatggt gatatacttt tcctctccgt tggaaggttg
    10021 gccttttgtt atcttcttcg catgtatgta tcttttattc gatattacgt ttaccatgaa
    10081 cgatatcgga tactgggcaa tgcttccgtc tttagcaaaa gatcagaagc aaagagctac
    10141 attgacgtca atggcaaacc tgtttgcatc cataggagca ataattgctt ttgcaacaat
    10201 acccattctg acaaacggtc ccaatgcaat aggcggtaac tccattacgg gatatatgtg
    10261 ggtatcgatt attattgcaa tactctttgt agccttccag ttgatgacgg caatcttagt
    10321 taaagaagca ccggtctcct atcgcagtga aggtgagaaa atagggctta aagcaatgtt
    10381 taaggtggtt ttcaaaaacg accagttgct gtggacttcg cttgtaatgt tgctgtataa
    10441 tctcggatct tcacttatta atgttttcgg tattatttat gtctacatgt atttcggcta
    10501 tgacggaatt aatataacaa tgtttgtggg ggcttatgct ttagggaatc tgttgattaa
    10561 tatagtgtat cctcaattgg ttaaagtcat gacaagaaaa cagattgcta ccaccggact
    10621 tataggagtt attatcggct acggattctt ctttataata ggtgtgctga ttcccatgaa
    10681 ttatctgctt ttatgtgtat ccggatttat tctttcattc ggtcaggcaa tgatatatat
    10741 ggttacaacc ataaatctta ccaactgtat cgaatacaat gagtataaga ccggtaaccg
    10801 tgatgaagcc attatattct caatacgtcc ttttatggct aaaatgagct cggcgttgca
    10861 gcagggtata gtcggtgcag cgtatctggc tctgggtata actgctatta ccaacggtat
    10921 atcggaagcc gaaagattaa gtaacatggg gcttatcagt gcggaagaga agagtacaat
    10981 gatagaacag gcattggcac tcggaaccga ttccatgaaa atgaatttca gaatattcat
    11041 tgtggtggtt ccgataatat tgatttcgtt ggcatatctt gtaatgatga aaaagaataa
    11101 gattgacgaa gaaacgtatg accggatgat gttggagata agccaaagga aacaggccga
    11161 atagacttaa agaacatata ttgcaaaaca gcttccgtaa cggaggctgt tttagttgat
    11221 atcggataaa tgtgtaaaat gtaaatgaat aaatgaaact gaaagtaata aaaatgttaa
    11281 tactgcaatt attgtattgc aattatgcaa aaatgattat ataatgcgga ataatttatg
    11341 ctgattagca tattaatgaa aggaatgaaa acaaattatg caaaaagaca caaatccggt
    11401 acttcagttc tccgacaaga gcaacctttt ggaacagtcc gcatataagt atcagttgca
    11461 ggacagagaa acacccaact tatacagata cctttatgat tataaaacgg tacctaaagt
    11521 accttttaat cacagaagcg tacccatgaa tatgcccaag gaaatatgga taaccgatac
    11581 cacattcaga gacgggcagc agtccacttc accgttcagt gtggagcaga ttgtgcatct
    11641 ttttaagtta atgcataagt tgggaggacc taagggactt ataaggcaaa gcgagttctt
    11701 cgtttacacg gaaaaagatc gtaaagccgt tgagcaatgt atgagtctcg ggtataagtt
    11761 tccggaagta acaagctgga taagagccaa tgaaaaggat tttgacattg taaaaagtct
    11821 cggtttaaaa gagaccggaa tattggtgtc ctgctccgat taccatatat ataacaagat
    11881 ggggcttacc agaaaacagg cgatggataa gtatctgggg attgtaaaga gtgttctgga
    11941 taaaggaatt aagcccagat gccattttga agacatcaca agggcggact tttacgggtt
    12001 tgtcgtgcct ttggcatatg agctgacaaa gttgtcggaa gaaaccaaaa tacccataaa
    12061 gataagagca tgtgatacca tgggattcgg tgtgagttat ccgggtgctg cattaccgag
    12121 aagcgtgcag gggataatat acggaattta tcactatggc ggagttaaga gcgaacttat
    12181 agagtggcac ggtcataatg atttctataa agtagtaacc aatgcatcaa cggcatggtt
    12241 gtacggagca tccggtgtga actgttcatt gttggggatt ggagaaagaa ccggtaactg
    12301 tcctttagaa gctatggtaa tagagtatgc ttcattgaaa ggaacgactg acgggatgag
    12361 cattcccgtt attaccgaga ttgccgaatt ctttgaagaa gaaataggtt atgaaatacc
    12421 gccgagaact ccttttgtag gcagaaattt caatgttacc agagcgggaa tacatgcgga
    12481 cggtatgctt aaggatgtgg agatatacag catattcgat accgaagcac tgcttaacag
    12541 accggcttcg gtagccgtgg attctcacag cggattggcg ggagtcgctc attggatcaa
    12601 ctcatatttc aggataaaag ataaggaaat agctgtaaaa aagaccgatt ccgttgttgc
    12661 caaggtaaag gaacttgtgg ataaggaata tgcagacggc agaaataccg ttatgggtga
    12721 cggcgagttg gagaatatat taaagtccgt tgactatgat gagtatgaga agttaagtcg
    12781 caagcatgca aggaagagaa aatagcggtg tagctgccgt ataatcgtat gctgtggcaa
    12841 tgttattttg aaaaaaattg tattagttgt ggacatttat cccgtttttt attatcctac
    12901 ttacagtgac ataataacgg gcgatggttc ctgctatatt atgtcaaaga gaggtattta
    12961 tgattaagag agttgttgtc gcattgggcg gtaatgcgat tttaactaag gatccaacag
    13021 cggaagggca gattcaggca cttcgtgaaa catcggagaa actggtcgaa ttgataaaac
    13081 acgattatga gcttattatc actcacggca acggtccgca agtgggaaat ttgcttttac
    13141 agcaagaggc tgcacactca aaatccaatc ctgccatgcc ggtagacacc gctgtcgcca
    13201 tgacacaggg aagcataggg tattggttgc agaacacatt ggacgaagca atatgtaaaa
    13261 gcggtatgga gaagtcggtt gccaccatat taacgcaggt agttgtagca gaggatgatc
    13321 cggctttcaa aaatcccaca aaaccgatcg gaccgttttt ctcaaaagag caagcggaaa
    13381 aaagagctga agaatcgggt gatacttata tggaagatgc aggtcggggt tatcgtaagg
    13441 ttgttccttc gcccaagccg gtttccgtta ttgaagccga tacaatcaga acacttgtag
    13501 agaacggagt catcacaatt tccgcaggcg gcggcggcat tcccgttgtc aaacgcggtt
    13561 caaaatatgt aggcgtggaa gcggttattg ataaagactt tgcagctgca aaacttgcag
    13621 agattgtaaa agctgattat ctggtaatat taaccggtgt ggaaaatgct tacataaact
    13681 ttaataaacc tgatcagaaa aaactggaac atgttaccgt tgccgaaatg aaacagtata
    13741 ttgcggagaa gcaatttgcc gcaggcagta tgttgccgaa aatccaagct gctatttcct
    13801 ttgtcgaaag cgtagaagac ggtaaagcat tcattacttc cttggataac gtcaatgcca
    13861 ttgcaggtag cggctctgcc acagtcatta caaaagacat atagcatcag ggtaccgaat
    13921 aaaaatgcgc caaaagccgt gtcggttatt gtgatattta aaaagtaaaa attaaaatac
    13981 agtgtgaatc tgcaatcttg cgacattgtc tgtggaatat tttggcaata tcgatattga
    14041 caaattcaat tttttttgta agaatggata tagatgaaag gggggacctt tctttctaaa
    14101 aatatcagga ggataaagtt atgaaaaaac taatttgcgt attattagtt gttgtttttg
    14161 cactgagtct cgttgcatgt ggtgacgtcg gaaaacaagg caagaaggat tttatcggta
    14221 tcattcttcc tacaaaagaa gaatcccgct ggcttaatga ccagaagtat tttgaagagt
    14281 acttcaaagg catgaattat gaaattctgt tcagccagaa taactccgct accgaaaaga
    14341 ccaatgtgga aaccttcatc agcaagggtg ccaaggttat agttctctgt gcgtacgatg
    14401 ctgcagctgc cgcatccgca gtcaacgaag caagcaaaga aggcatcacc gtgatttctt
    14461 atgatcgtct tatcatgggc acggataagc tggattacta tgtaaccttc gacagctgct
    14521 ctgtaggtaa ggctcaggct gattacctcg ttgagaaagc cggttcggag aagggcatca
    14581 atctttacat gtactccggc gctctgacag acaacaactc cgttctgttc tttgaaggcg
    14641 cttggaaggt tcttcagccc aagattgctg acggtacatt tgtagtaagg aattgcgaaa
    14701 aggcaatcca attcaaggat aagaacaatc tgacccgtga agaaatggtt tccattatgc
    14761 agaccattga ctccgagtgg aacatggagc gttgcaaatc tttggcagaa gctaacctga
    14821 ccgccgcagg cgataagggt aaaggtaaag tatacgttct gggaccggct gacgatgatt
    14881 gctgccgtgc tctttccgat gctttcttag ctgacccgaa ggtaaccgaa ctgaagatta
    14941 ccggcgctga cggcgttgag gcttccgttc agtacattat tgacggcaaa cagtccatga
    15001 ccgtttataa agacacccag gctctggtta aagctaccaa gcaaatcatt gaagctctgc
    15061 aaaagggtga gaaaccgaag accgataccg tttacaacaa tgatgcgaaa gacgttccga
    15121 ccatacaggc cgatgttgtc accgttactg cagacaatat cgcagagatt ttctttgagt
    15181 caggcgttta tgacggtagt aaatataccg gttggaaata atagtataat tctgcaataa
    15241 ttttaccggg atatgtgcct tcgggcgtat atcccggtat cattaaagga ggacattagt
    15301 gaacgattat attttggaaa tgcacggtat ttctaagtca tttcccggtg ttaaggcgct
    15361 gattgatgtt aatttttcgg taaagcgcgg agaaattcac tgtctggtcg gtgagaacgg
    15421 agccggtaaa actaccctta tgaaggttct ttccggtatt catccgtatg gaagttatga
    15481 gggcgacatc gttctgaacg gccagatcat gaagttcaat accatccgtg aaagcgaaaa
    15541 taccggaatt gccatcatca atcaggaact ggcattgatt cccgacttaa ctgtcgccga
    15601 gaacatcttc ttcggccacg aaatcatgaa gaggggattg gttgactgga acgaaaccta
    15661 taaacaggca gatatttatc tgaaaaaggt aggtttgaat attaatccca ggtcgctgat
    15721 gagagaccat cgtgtaggca cacaacagtt ggtggaaatc gctaaggcgc tttcaaagaa
    15781 tgccaagctg atgattcttg atgaacctac ctcatcactc aatgaagaag acagtgctaa
    15841 tctgctgcgt cttttgaagg aacttcagaa gcagggtgtt acgattatca tgatttccca
    15901 tcgcttaaag gaagtggtat ccattgcgga aaccattacc atcttgagag acggcgcaac
    15961 cgtctacacc atgagagcgg atgaggaaga aatcaccgag aacaagatta tcaaatacat
    16021 ggtcggacgt gaaatggaca atatctatcc gcatcgcgag agttgtgtta ccgatgaggt
    16081 tttattcgag gtgaaagact ggtgtgcata taacgctaag agcggacaga ccgttctgaa
    16141 gaacatcaat ctgaaagtac ataaagggga aattatcgga atggcgggcc tgatgggtgc
    16201 cggcagaacg gaattggccc tcagtatttt cggtaatacg ccggagtaca agattactac
    16261 cggtgagcta tatcttgaag gaaacagagt acgtttctcc tcgcctaagg atgctataaa
    16321 gaaaggtgtt gcgtacgcta ccgaagaccg caagaaaaac ggtctgatat taattcagga
    16381 cattaagttt aatacatcct tcgcaaatct ggaccgcatc cgtaatagag gagtcattaa
    16441 tcggaatgag gaggttgttc aggcaaacaa gctgaaggag agcatcaata ttaaagcgcc
    16501 ttccgtgcag cagattctca ggaatctttc cggcggtaat caacaaaagg ttgttctggc
    16561 aaaatggatt ttcgccactc ccaaacttat gattctggac gaacctacac gtggaatcga
    16621 tgtaggtgcg aagtttgaaa tttatacatt gatgaatgag atggtgaaac agggcatgtc
    16681 tatcatcatg atttcctccg aacttctgga ggtcatcggt atgagcgaca ggatttatgt
    16741 catgcatgag ggaaaaatca ccggcgaact tagcaaatcg gaagccactg aacagaaaat
    16801 catgcatttt gcaaccaata cggaggaatt atcatgagtg aaaataaaaa aacgggcaga
    16861 gaatatggaa aaacgctggt gacaaaccta cgtaactatc tgatgtacat tgctcttgca
    16921 gtcattttta tagtattcca gattttgagt aacggatcat ttttgacagc aagaaacatt
    16981 accaatctga ttaaccagac cggttacatt gctgttatgg ccgtaggtat gacattaatc
    17041 ttaatcatcc gccagattga cctttcggtc ggtttcgtgg caggattctt aggagctgcc
    17101 gcagccagac ttctgaaagc cggtgttgcg gtacttccta cgattcttat tgttttggcg
    17161 atgggtgtca tgataggact ggtacagggt ctgattatcg gtaaggtggg cgttcccgct
    17221 tttattacca cactggcatt cgaattcggt ttccgcggtc ttctgacctt aattaccgag
    17281 gccacaggta ccatccccat tcagagcgat gtgttctatg agttgtccaa cggcttcatt
    17341 cccgaattgt tcaagattgg agacctaagc ggcagttgcc tgattgcagg tgcgatatcg
    17401 atggcactga ttgtcttctc ggagatacga aaaagaaaag aactgaagcg ttacaaattc
    17461 gaagatatgc ccgtacttct cttcatactg aaacttttat tctttgtagc tttgattgga
    17521 acactgacag ttgttcttgc gggctatcgc ggcatcagct ggaccatcgt catcgtcgtt
    17581 atagtgatag ccatctacag ttttgtaatg aataagacca aggtcggtcg ctatatttac
    17641 ggtatgggcg gcaatccgga agcagcgcag ctttccggtg tagatataaa gagaatactc
    17701 atcggaagct ttgcttcaat gggtttgatg gcggcactgg gcggtatcct ttacacatca
    17761 cgtctgggtt ccgcaactcc gaccgccggc atgggctttg agttggatgc catcgcatcc
    17821 tgctatattg caggtgtttc cacaagcggc ggcgtaggaa gtgtcgcgaa ctccgttatc
    17881 ggtgcatttg ttatcatgtc actgaccaac ggactgaacc tcatcggtgt cggtatttca
    17941 taccagtatc tgattaaggg tattatcttc attcttgcag ttgctttgga tgtaagatca
    18001 cgcggaaaga aggctctagg ctgagtatag gagaatataa aaaaactaaa gcggttccgg
    18061 aagattcttc cggaactgct tattgccgaa taaatatatt gacaaataaa tagcgagcca
    18121 ttagaatagt aaaaggcttc gtttggtcat ggtgggtata gctcagttgg ttagagcgct
    18181 aggttgtggc cctagaggtc aagggttcaa atccctttat tcaccccatt ttaggagatg
    18241 atgggatgta gccaagtggt aaggcaacgg actttgactc cgtcacccgt aggttcaaat
    18301 cctgccatcc cagccagtct tgcgggacat tagctcaggc ggtagagcac ttgactttta
    18361 atcaaggtgt ccggagttcg agtctccgat gtctcaccag aaggccccat gtatatgggg
    18421 cttttttgtg cccgtttttt tcggttgtat tgctgatcca tactatttac acataaatgt
    18481 cttgcatatg ggtatgtttt agtatattat tgaagtgaaa gtaaaacgaa accataatgt
    18541 aaccaatcga aaccctgaag caatggaagt aactgtggaa gagagaaaag aaaaaatatt
    18601 ggatatgctt aacagagacg gcagagtcaa ggtaaacgag ttaagcaaat tatttgatgt
    18661 ttcggaagta accataaggc tggatctttc cgacctggaa gaaagcggcc ttctttccag
    18721 agtatacgga ggagcggtat catcgtataa aagctactac aatatgagtt ttaaacaacg
    18781 ctcggctgca aacagcaatg agaaaaaagc aatagcttcc agagtgtcac aaatggtggc
    18841 ggaaaacgac acactgatga tgaactccgg taccacgaca ttgattacct atatggcttt
    18901 acctaaaaat atgcacctta ccgttataac caactctgtg gctatagctc aggaagcatc
    18961 aggtaacgac aaccataaaa tactgcttct cggaggtaac gtgaataccg attaccagtt
    19021 cacctgggga gaagattcgc tcaaccagct ttcggcgtat catgccgata aagctatact
    19081 ttcggtagac ggaataagcg taaagtccgg tcttacgaca tattatcatc aggaaagaca
    19141 tattacctct cagatgattt ccaattccga tattgcgata gtaaccgcgg attataccaa
    19201 gataggcaga gcggcacttt ccaaaataac ttttgcagac agagccgact gtattattac
    19261 caacctgaat gcatcggcag aagaaatacg gatgttgtcg ggtaaaggaa taaaagtgca
    19321 tttagtatag agagacgacc atgaaaacat atattaaagc gggatggcgg gaaaacagca
    19381 taacacctac aaaaagagca agtcttgcgg ggcaatttta tgagcgcata tccgagtatg
    19441 tggagaccga tataacggtt actgctttgg cggtatcttc ggaatatgac cattttgtaa
    19501 tctgttcctg tgatttactg tatgttcagg aatctcttat ggataaggta agacaaaaga
    19561 ttgagggaag atgcgaaggg ttggatccca tgaaggtaat cttaaatgca acacatacgc
    19621 atacttcaat agattacggt aatccgcaaa gcaagtacgg ttccgctctg gatgtgttga
    19681 aaaaatatgt accggaagat tttacctata ctgaaaaagt gcgtgatgac tccataatac
    19741 aacctgagga agccgagatt tttttagcgg acagtattgc cgatgccgta atcaatgcat
    19801 ggaataaccg aaagaaagca tatttctcca atgccttcgg aagagctccc gtaggtatgt
    19861 gcagaagagc ggtgtataaa gacggaaccg ctttgatgtg gggagatacc caaagaggag
    19921 atttcataca tttagaaggc ggtaatgact cgggaataga gttgatatat ttctatgatg
    19981 aaaaagagca ccttaccgga attgttgcca atatagcctg tccaagtcag atattggagc
    20041 acagaagctt catatcttcc gactactggg gaaaggtcaa aatactgttg agaaagcaat
    20101 tgggtcccga tatattcctg ttggctcttt gcggtgcagc gggtgaccaa tgtccgagag
    20161 accttatacg gtgggtacct ccgataactc ctatagatga tccgaatatt acgaggaaag
    20221 ataccgtcaa aagggttgca gacccttcca tgtttgatat agagggttcg tggaaagcgg
    20281 caaaaaggat agccaacgaa ataatcgacg tgtattccga agccaaagaa catgttaccg
    20341 tcaatgcggt tataaaacat gaatgtattg atttacagtt accgttgaga aaggcgacag
    20401 agaaggaata tgagtatgcc cttttcaaga taaaggaatt tgtaaagaat aaagaaagta
    20461 ctacatatga ttttaacgat aaggcaaaaa tgcatgtgca cgccggtacc gttgcaaggt
    20521 atatgtatca gcagaaggaa gataaatgca atatcgagat gcatgtaata cggatggggg
    20581 atattgccat tgccaccaat ccgtttgaac tgtttctgga ttacgggaat attatgaagg
    20641 cacgctcaag agcatcgcag acattgatag ttcagcttgc ttgcggatgc ggtaattatc
    20701 ttcctaccaa aaaggcggaa caacacggtc attacagtgc atatatatcc agcgggataa
    20761 cagggcatgc cggcggcagg aaacttaccg aagtgacttt ggaaactata gataaattat
    20821 gggaagagga ggacaaatga atttactggg actggatttc ggcacaactt cattaaaagc
    20881 ggcggtattc aataaagaag gtcaatgtct ttttgtccac gaagaggcat atgaacttat
    20941 acataatgac ggatttatcg aatttgatgc aaaagagtac cttaatatac tcaaaagagc
    21001 aataaaagcg gcgaccgata agttttctat atatgcaatg gccatagaca cgcagtgcga
    21061 gacaataata ttagccgacc aatcgggcaa tccgctttgt aacgccgtgg tatggctgga
    21121 tgacagagcg aagcagcagg ctttgaagat aaaagaagat ttcggtaaca gacaggtata
    21181 tgaagttacc ggccagtgcg atatatccgg tatatggccc gcctgcaaac tgctgtggtt
    21241 caagcaaaac cgaccggata tatggaaaaa gacgggaaag atttttcttt tggaagatta
    21301 tctgttatat aaccttaccg gaaagtttgt cacacaaaag actttacaat cctcctctct
    21361 gtatttcgat atagttaacg gctgttggtg gaaaaccatg ctggattaca taggtataag
    21421 tgaagatatg cttcccgctt tgtacgacag cggagcatat gttggagatt acaacggtat
    21481 acacattgca acatcggcaa tggatcaggc tgcaggtgca ataggagcgg gtgtatacaa
    21541 agaaggagtt ataagtgaga tgacagggac tgcaatggcc atattcagcc cgaccgataa
    21601 tatgccttta taccatgaag atataattgt tccctgccat tacaatgcaa cgactaaata
    21661 tgccgtaatg ccgtggatgc ctacggcggg aatggcatta aaatggttta aggattgttt
    21721 tatgtacgat atgacttatg cacaattgga tatgcttgca aaggatgcgc ctccgggatc
    21781 ggacggactt atgttttttc ctcatctttg cggctccatt catcctatac tcaatcctca
    21841 tgccaaaggt tcattctacg gaatcggact tgcgcataaa caagaacatt ttgttcgggc
    21901 tatattggaa tctatagcat atatgctgaa aagctgtata gcttctcttg aatgtgattt
    21961 ggacgcaata cattccattg gagggagtgc caaatccgat atatggtgtc ggataaaggc
    22021 cgatgttacg caaaaaaaga ttattacttt aaaggaaaga gaacctgcat gcaaaggaag
    22081 cgccatactt gccggtcttg caaccggtgt gtactcgtcg gtagggcaag catgcgaaaa
    22141 aacccttgca accgacaggg tgatactgcc cgaaagagat gtatatgcgg gattatatga
    22201 agaatatatg aaaataaatg aaaaaatata tggaggagag tgtaaatgag taaagtagca
    22261 tttttaggat tcggtgaagt caatactccg agagaattga ttgtgaagaa gtgtaaaaaa
    22321 gcggagaatg cgttaaagaa agaagggctg gaccttataa gcatatatcc gataaccgat
    22381 gattccgaaa gagaggatgt gcttaaagcc atcgataagc tttcaaaagc atcgttcggt
    22441 accattgttt tgtgtgttgc cggatggatt ccctcacatg caataatcga tatcatcgaa
    22501 agattccgac atatccctat ggtattgtgg gggctttgcg gctggatgga aggagacagg
    22561 ttggttacca ctgccgacca ggctgcaacc agtgcgataa gaaaagtaat ggaagatttg
    22621 ggatattctt tcaagtatgt gtatgatatt gtcggaaaac ccatgaatac ccaaaaggtt
    22681 gcggattttt gtaaagcatc ggaagcggct gccaaattgc gctcttccag agcgggaatg
    22741 atgggttaca gggatatgaa tctgtacggt acaatgtttg atgcaatcag acttaagaag
    22801 tatatcggaa cggatattga aagctttgat atgctggaag tgaacagaag ggcggagaag
    22861 gttaccaaag aagcgattga caatgttata gagcaggata tgtctaagtg ggttttcctt
    22921 aaaccgtgtg atagagagac aaaggaaaag gcggcaaaat attatttagc catttcggat
    22981 atatgtaaag aaagaaagta tgaagccgtt tcactgaaag atgtcgacgg aatgaaaaag
    23041 tttctgttct atcctccggc gcccatattc atgctgctgt ccgatgtgga cagattaagt
    23101 acaattccgg agaatgactc ttacggaatg gttactcagc ttatggttaa gtatctgacc
    23161 ggtcagtgcg gggcatatct ggagttctat gagtatatgg aagactgcgt tatagcggga
    23221 gtacccgatt atgtgcccaa ggagataata gacggagata ctcttgtcat gcctgcggct
    23281 ttcggactgc tttcacaagg tatactcaat gtttccaagg taaagcaagg taaagtcaca
    23341 ctttacagac ttgcggaaag caaaaacggt ttctgtatgc acatagtgca gggaagagcg
    23401 gaatcaccgg gcaagtggga agaggccggt tggactcagc ctgcaccgca acttcccgga
    23461 cttaaggttt atatggacga tgtggaagca tttacctcaa aagtaatgag ccagcactat
    23521 ataatcgcat atgaagacaa tgcagacaag atgatagatt tatgtaaatt attgaatata
    23581 gaggtaataa aatgagtaag gatgttttgc aggctgctta tttttcttta gatacggaga
    23641 aaaaggaaat ttcaaggctt aaggactatc tggatgagca atgcttttta aaagcggtgg
    23701 atgctctggc aaactgtaag agcattatga cggtagcttc cggttcatcc ggaattgcgg
    23761 caaagaaatt tgcacattct ttatgctgta taaacaagca tgccatgttc atgcccccga
    23821 gcgaggcggt acacggcgga ttgggtgctc tggatgaaca ggatgtaatg gttatggtgt
    23881 ccagaggcgg aaaaacagtg gagcttcttc ctattattac cggatgtaaa aaaaagaagg
    23941 ctatgcttat aggggtaacg gagaatgatc agtccatact tgcaaaaaac tccgacatat
    24001 tgctgaaact gagtatagaa agagaaagcg atcctttggg actgatggcg acatccagct
    24061 atgtggctac aattgcatta tttgatgcat tgttggcttc attaatagta aaaacaggtt
    24121 ttaccgccga gatgttcggg gttattcacc ccggcggagc ggtgggggaa ctgctgaata
    24181 aataggagag aatatgtata aaggacttaa aatttccgca cctttttttg agataggacc
    24241 caaagcatat ctctacggag agaaaatgct tgcattggca aaggtcatag acaaggttgc
    24301 gactaagtat gatgtggatg taatagttac accgcagtat accgatatat ccttacttgc
    24361 gcacaatacc gaaagaatat cggtttttgc ac
//
LOCUS       DAOSOM010000031        21426 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_2462_length_21426_cov_4.922559, whole genome shotgun sequence.
ACCESSION   DAOSOM010000031 DAOSOM010000000
VERSION     DAOSOM010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 21426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21426
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2462_length_21426_cov_4.922559"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="PLF14_07440"
     CDS             <1..525
                     /locus_tag="PLF14_07440"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015010120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT71157.1"
                     /translation="EKYSSLKGMTYYTQMNQRIYELEKVNPGIERIGTIRLVNNNDMH
                     FFPYWLSAFYASNVYGQTVMNIPESEREAQYRIDMKKIYILEVNGIPVSMAGYNRELP
                     GSIGVAYVYTPPYFRNKGYASSAVAQVSQLALDKGYKRCVLYTDLSNPVSNSIYGKIG
                     YVPVCDSLMLQFNN"
     gene            723..1043
                     /locus_tag="PLF14_07445"
     CDS             723..1043
                     /locus_tag="PLF14_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71158.1"
                     /translation="MKKRIIKGIILSVLCLILLASLTSCAMGLSGTYKKGTDLAYETY
                     RFSGNKFYYEVGALGVKVGGHEGTYKIKDGKITFIYDDSEHSYKFEKGKDYIIINGAS
                     YDKK"
     gene            complement(1470..2780)
                     /locus_tag="PLF14_07450"
     CDS             complement(1470..2780)
                     /locus_tag="PLF14_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71159.1"
                     /translation="MKTKIKVVVQTKKKDVPGWPHINYDYEKETQSVLNVLSQNTDME
                     FDVFKYTHVSQAETAYPDDLKKYDGVLVLLMTNWINLDVFYARKAKEGLPCIIADIPF
                     CGSGSVLVRSIHTVREEHLPVPVIASANFNDIAKAVRVFVGIKKVKQSRILVVSNNID
                     KETQAAAEKIWGCTFINCNSEELMKRYKNIKDIDAEVIKDKWISQSEGVLEATNQDVS
                     ESAKLYLAIMEMYKEKKADAVTIDCLSLSYNDIYGNNLHMYPCLAFFQMCEDGYVGVC
                     EADIDSTITSIFTKAITGRYGFVSDPVIDTSSDQIIYAHCVSCRKLFGEQDKRLCKYY
                     IRSHAEDKKGAAVQVIFPANEQLTTVNINNVDKTACIHSAVSVGNYGGDAGCRSKLAA
                     LCKSEEILNNWMPQWHRVTVFGNYTKEFIYLFKIMGFNLVTEDK"
     gene            2922..3716
                     /locus_tag="PLF14_07455"
     CDS             2922..3716
                     /locus_tag="PLF14_07455"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOT71160.1"
                     /translation="MLDIENYDISINSCAFTKCDEHWNWVTAKQGFKDYDLWFITGGK
                     GYIEYLGNKYELHEGKCFLLAPNVQYVAYHDIADMLSVIHIHFDFVKNGMKAYPFGVI
                     DSHTGSTNLIIRSLEYVMFLYNIKDYQRAYIWFKAVLCDLFGECASANDGIDKGIHEV
                     IYSICRVIGNSVNIPNLAEFAEQYSYSVSYLGKMFTKITGMSYTDYVNHTKLNKAKVL
                     LCSTNKSLSRIAEKTGYYDASYFSRQFKKSTGISPGKFRKNSTGLE"
     gene            complement(3835..4353)
                     /locus_tag="PLF14_07460"
     CDS             complement(3835..4353)
                     /locus_tag="PLF14_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009007772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71161.1"
                     /translation="MSNINYNYFVEGETEACLIRAIRQKYIQSGRITKINLLEKDIPS
                     GFIKTIKPFTILIIVFDTDVDDMKLVERLKDNTGKLKKSSHVKDVILIPQVNNLEDEI
                     KYATDISEIKDLLQSKSNKDFKRDFCKCNNVTDCLVKHSFNIEKFWSRKARNKYSIFE
                     NDGEKIKLKHRR"
     gene            complement(4346..5446)
                     /locus_tag="PLF14_07465"
     CDS             complement(4346..5446)
                     /locus_tag="PLF14_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT71162.1"
                     /translation="MVVMNLELDNLLGFDDFKINFSYPRKIVNSTIKGEFLPNKTNFR
                     FKKVNILLGVNASGKTTLGKAMMHIFNFIAYKSPGRLFESVKDKKRKAFFSIDFLRDE
                     EMLYRVNCSLERSDKKEAEPKLSLEVYTAPIGRRDSYEICVRKLEQVKVKDDNKSSLD
                     VLDSIKSFGWHFTFPDIGNTDIRIDKDVLSLDILKRVLKTLDTSITDVQKSDEVENSY
                     VIRSRNGDVFIQNGKIIEDILSSGTKMGIDISYVISSICKGMHGFYYVDEKFTFIQTD
                     IEQFLLGIMIDKLGPLEQLFFTSHNLDLLDMDLPVHSFTFLKKDENIEAVYPSDFIKK
                     NDISIRNAFVNDVFGTTPDLSQLWDLGEFCDE"
     gene            5743..5802
                     /locus_tag="PLF14_07470"
                     /pseudo
     CDS             5743..5802
                     /locus_tag="PLF14_07470"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            5849..6154
                     /locus_tag="PLF14_07475"
     CDS             5849..6154
                     /locus_tag="PLF14_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT71163.1"
                     /translation="MTTWADYKNNVRETNPEIGKDIDEVEAISKIVGAMIERRHDLEL
                     SQRELADMCGIPHSSVARIESGSSTPNLRTLLKIFRELGLNLVAEPAVGSSPNDVSL"
     gene            6311..6529
                     /locus_tag="PLF14_07480"
     CDS             6311..6529
                     /locus_tag="PLF14_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOT71164.1"
                     /translation="MMPLISVKNGEKAVIKKVTGSNEVRKYLTDLGFVEGSDICVITE
                     IMGNMIVNIKECRIALNRELASKIMVEV"
     gene            6536..6757
                     /locus_tag="PLF14_07485"
     CDS             6536..6757
                     /locus_tag="PLF14_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005362589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HOT71165.1"
                     /translation="METLDKVKAGQSVTVRKIRGTGPVKKRLMDMGITQGTEITVVKL
                     APLGDPIEMKLRGYDLSIRKADAELVEVE"
     gene            6808..8943
                     /gene="feoB"
                     /locus_tag="PLF14_07490"
     CDS             6808..8943
                     /gene="feoB"
                     /locus_tag="PLF14_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOT71166.1"
                     /translation="MKIAIAGNPNSGKTTLFNALTGLNQFVGNWPGVTVEKKEGKLKK
                     KKNVHIMDLPGIYSLSPYSLEEIIARDYLVNERPDVIINIIDGTNLERSLYLTTQLAE
                     LGIPMVIAVNMMDSVDKAGDKLDTKKLSEQLGYPVVGISALKKRNIQQLIKETLKAGE
                     DKGSVPVMKFNSVAEEAITGIKEIVGSKADGINDRWLAVKFFERDEKVSDTYKLNKSE
                     AEAVEKYIKKCEEKLDDTSESIIANERYKYAAKVASSSFVKKDKGALTVTDKIDKVVT
                     NRFLALPIFVVVMFIVYFVSVTTVGAIVTDWTNEVLFAELIAPGVEDWLVSINTADWL
                     TGLIVDGIIGGVGAVLGFVPQMFVLFLFLAFLEACGYMARIAFILDRLFRKFGLSGKS
                     FIPMLIGTGCSVPGIMASRTIENERDRKMTIITSIFMPCGAKLPIIALIAGALFNEAW
                     WVAPSAYFMGIAAVIISGIILKKTKPFAGDPSPFVMELPAYRWPTVGNVLKSMWERGW
                     SFIKKAGTVVLLSSIFIWFTSSFGIIEGKFTMVEDLSDGFLSKIGTALAWIFKPLGFG
                     EWRLTVATIMGLVAKENVVSTIGILYGFAEVSESGEEIWELLAASYTQIAAYSYLAFN
                     LLCAPCFAAIGATKREMNSGKWTWFAVGYQTVFAYAVALIIYQIGTLISTGAFGLWTV
                     IAFIVLGLMLYLLFRPQPKRSLTGERITA"
     gene            8940..9104
                     /locus_tag="PLF14_07495"
     CDS             8940..9104
                     /locus_tag="PLF14_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009446349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HOT71167.1"
                     /translation="MKEFILENLATVIISLVLLSVVTLIVVSMIRNKKKNKSICAGCP
                     MAGNCSKGKG"
     gene            9312..10250
                     /locus_tag="PLF14_07500"
     CDS             9312..10250
                     /locus_tag="PLF14_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOT71168.1"
                     /translation="MNNNYSSIQNLLHQKADCQARLNLLPYDGNPEIKERDGRKYLYV
                     RKRVGSRLSSTYAGIYSDELYQLLVRNAREAREIRRNIRRLDKELALLGYSDRGLTPK
                     VQLNLDFARINIKSSIFDQAILEGVATSFSQVEDIIEDNTVSGMTASDVQKILNLKHA
                     WEFILDKDVIAYPSDYSILCHIAKLVNEGFFQDGGRIRGVPVTIGGSNYIPPMPIESV
                     VKENIQAILQADIPSDEKAIKLCLYCMKAQIFNDGNKRAAIIFANHYLISQGGGIIIV
                     PEKNVPQFKTLLIAYYEDRDKGEITEFMKKQCIKTF"
     gene            10476..11375
                     /locus_tag="PLF14_07505"
     CDS             10476..11375
                     /locus_tag="PLF14_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT71169.1"
                     /translation="MEGSNLFYDQLLSGDEQLSFTYLEKKLREIIKIPALDNDILRSL
                     GFYTKDMKYNNAASIFADKNNMFGVDIVRFGNSINEILDRETIYKNCILEQYDRALVL
                     YRKYYQYEVIEGALRERRERLPESAFREALANALVHRDFDVNAHIRIAMFKNKIEISS
                     PGGLPSSIKKEDYLNGTISVLKNPIIGNIFFRLDLIETLGTGIRRINQAYRDYDIKPD
                     YMIFDTSITVELPTTTVEYEITGEESDIVDALRGGRILTCSEITDKTGYNKSKVLRLM
                     KNLTDKKYVSVIGGGRSTKYKLR"
     gene            complement(11462..13000)
                     /locus_tag="PLF14_07510"
     CDS             complement(11462..13000)
                     /locus_tag="PLF14_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71170.1"
                     /translation="MKKLISILLIIALLAASILPVSAAPESSKKEEVIYGILDEYGQI
                     QQVYVINSFNQGSITDYGTYDSVKNLTTNDKVTVQDDLITIDSKAQKLHYQGTLDTKN
                     LPWIIEVKYLMDGKELKADEIVGKSGKAEIRISVKPDTKSDKFFYSNYTLSITVKLDE
                     RIFSDIQTQNGVIATAGMYKQITYTVLPGKNADINIKADAKNFELDSVTFGGVRMIMD
                     MPVDTHSITERFNELTDAIKKLDDGAEELTDGFEEYVSGLTQYTEAQASFRDGIAQLS
                     QGISQVYQGASQLAGGLDALSRQGAPLAQAIHGIVLKTFQTANGTIQQMGLPLPELNE
                     QNYAAILENVPELAPLKAELDGVIQLKAGMDMYTGSVTQVSQAATELKNGICKINTSA
                     ATIKQYADTLTESAATLQSASVELKEGLLKYRDGVRTLHYETMGLDSKIRKEIDNMID
                     EIFGDADIVKSYVSDKNKSVESVKFIIKTKAIELEKEAKTPTNGQAKITFWQRFLNLF
                     GLGK"
     gene            complement(12997..15078)
                     /locus_tag="PLF14_07515"
     CDS             complement(12997..15078)
                     /locus_tag="PLF14_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008634454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOT71171.1"
                     /translation="MEKFTSGIVKRRKLIIIIFITLAVLCGVMAMGVPVNYNLEDYLP
                     KDSNSTEGMSLMEKEFDEDFPTASIMVRNVSVHQALEYKEDLENISGVTAVTWLDDVI
                     GQDILHSTPLEFLDSSVTEAYYKNGNALFSVSIAPGFEVEAVDGIYELIGEENAASGS
                     SVSKAVVQKLSATETLRSLLILVPLIIIVLLLTTSSYIEPLLYLAALGVAILINMGTN
                     LLFDDISFITYTVSPILQMAVSLDYAIFLMHSFKEYREQYEPEEAMKRAIKKSFTSVA
                     ASAATTVIGFLALLFMRFQIGADLGINLVKGVALSFISVMIFLPALALSFVRIMDKTQ
                     HKTVRMDLKRVGQWLMKLRIPLLIISILVIIPCFLAKDKIPFEYGSGAGIKASRAEED
                     KLAIEEQFGKINQMAIIVPKGGNGSLNMLSNELEDIEGISAVISYASYIGPAIPEGFV
                     EGQDSMFNSENYSRIILYTDYEIESKETFAAIEKIRATAEQYYDEVYLVGESASVYDI
                     KDSITADNTLVTLIAVLGIFLVILISFKSLSLPFILVFTIEAAIWINLAFTYFQGSSL
                     NYIGYLVINTVQLGATIDYAILITDRYMHNRTLLPAKEAMAKTLANNIVAVMISAGIL
                     ASAGFALTLTSSNPIVRELGGLLARGTILSFVMVSTVLPALLILFDKVIRKTTYKNGF
                     HLSKKTGGDIK"
     gene            15239..15811
                     /locus_tag="PLF14_07520"
     CDS             15239..15811
                     /locus_tag="PLF14_07520"
                     /inference="COORDINATES: protein motif:HMM:NF025636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HOT71172.1"
                     /translation="MKKQKKEDRRVKYTKMVLKESLIDLLSQKDISAITIKEICEEAD
                     VNRTTFYAHYADQYDLLRQIEDELLQNLKAYITVFKQKKADAIFVDILEEMFEYIKAN
                     AKLCKLLLSKEGNLDFQKRIMMLVYDTNLVAKPNETLKKGEEEYVYSFVITGCVGVIH
                     KWLEEGMKHSSRIMAGMIINSVKNLPVTFE"
     gene            complement(15843..16556)
                     /locus_tag="PLF14_07525"
     CDS             complement(15843..16556)
                     /locus_tag="PLF14_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOT71173.1"
                     /translation="MNEDKRFAVLIDAENVSEKYIKYIFDELARSGTITYKRIYGDWT
                     RPELSSWKKILLDYSITPIQQYGYTTGKNATDSAMIIDAMDILYDDNVEGFCIVSSDS
                     DFTRLATRLREAGKYVVGMGEKKTPAPFIASCNQFKYLEILGQEENNNKQKDMEDSIT
                     PLEKVIKAIETIADDISDDDGWSPLSDVGNILSKMFPDFDVRNYGFSKLTPFVSSLKK
                     FDIKSVNLNNIKLIYIRKK"
     gene            complement(16604..16897)
                     /locus_tag="PLF14_07530"
     CDS             complement(16604..16897)
                     /locus_tag="PLF14_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005838004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOT71174.1"
                     /translation="MTYSIITTEKFDKAFKKLDRQTQKIIQSWIDKNLVNCENPRIHG
                     KGLTSEKTGYWRYRVGKYRILADIRDNILILVLIDIGHRDKIYKEEITQSRHK"
     gene            complement(16894..17118)
                     /locus_tag="PLF14_07535"
     CDS             complement(16894..17118)
                     /locus_tag="PLF14_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004012879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="HOT71175.1"
                     /translation="MATISLRITDEELDILKAYAKINGKSLSEVVRNVMMEHIEDQFD
                     MQVFAEYEKEKSEGKLKTRPVNKLWEELQL"
     gene            complement(17180..18099)
                     /gene="argF"
                     /locus_tag="PLF14_07540"
                     /pseudo
     CDS             complement(17180..18099)
                     /gene="argF"
                     /locus_tag="PLF14_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005352843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
     gene            complement(18099..19259)
                     /locus_tag="PLF14_07545"
     CDS             complement(18099..19259)
                     /locus_tag="PLF14_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009351042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOT71176.1"
                     /translation="MDIKALDKQYIANTYKRFDLDLVRGHKQFLYDSNGKQYIDMGSG
                     IAVNSFGVADEQWKQAIIAQLDKLQHTSNLYYTYPQVELAKLLCKKSGMSKVFFANSG
                     AEANECAIKTARKYSSDKYGQDRHTIITLENSFHGRTVTTLSATGQDVFHKHFGPFTQ
                     GFLHVKVNDTKEMEKHIKSGHVCAVMLELIQGEGGVIPLDKDYVANTEKLCKENDVLL
                     IIDEVQTGIGRTGAFYSYMRYEIQPDIVTSAKGLGGGLPIGATLFNEKTHNVLDYGSH
                     GSTFGGNPVCCAGAISVVSRINNKLLKDVLEKEKIVRELLQGAKGVQSISGTGLMLAV
                     KTEKDAKEIVEKCMANGVLFLTAKDKIRMLPPLNICKKSLAKAVKVLKEACE"
     gene            complement(19268..20119)
                     /gene="argB"
                     /locus_tag="PLF14_07550"
     CDS             complement(19268..20119)
                     /gene="argB"
                     /locus_tag="PLF14_07550"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002852078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOT71177.1"
                     /translation="MTLSNAQRAHILVEALPYIQKYNNKIVVVKYGGAAMTEEHLKKD
                     VMRDIVLMSSIGMKVVLVHGGGPEITGMLNKIGKKTEFVNGLRVTDRETADIVQMVLC
                     GRINKNLVSMLQSYGGKAIGICGHDGSLIKARKISEQLGFVGEITDINKSVIEDLLEK
                     GYIPVISTVGCDDDMNTYNINADTASSAIASALNAECLISMTDTPGLLSDKNDQKSLI
                     SKISVSDASRLIREGAIEGGMIPKVQCCIEAVRRGVKRVFIIDGRIPHSILIETLTDE
                     GIGTMFA"
     gene            complement(20133..21350)
                     /gene="argJ"
                     /locus_tag="PLF14_07555"
     CDS             complement(20133..21350)
                     /gene="argJ"
                     /locus_tag="PLF14_07555"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HOT71178.1"
                     /translation="MEYIKGSITAPVGFRANGIHCGIRKNKTKRDLALIVSDTICTAA
                     CVYTKNLVKGAPITVTQQNVADGYAKAIICNSGNANTCNENGMEIARGMCNLAAKELN
                     INPADVIVASTGVIGQKLSLDPIASHMKELCDGLSKENGIHCTEAIMTTDTRVKEVAV
                     SFKINGKTCKMGGIAKGSGMIHPDMATMLAFITTDVNISKELLQKALSEDVKDTFNMI
                     SIDGDTSTNDMVSVLANGQAKNSEIKEKNKEYEIFKKALNAVTSYLSRMIVKDGEGAT
                     KLIECKVINGKTKDICKTVAKSVISSSLVKAAMFGADANWGRILCAIGYSGADVDIGK
                     IDVSFSNDKETTVKVCEKGFGVQFSEQLAKTILSYEEITVLINLNSGDAQATAYGCDL
                     TYDYVKINGDYRT"
BASE COUNT         6185 a         4221 c         4154 g         6866 t
ORIGIN      
        1 gagaaatatt cttcgttaaa gggaatgaca tattatactc agatgaacca aaggatatat
       61 gagttggaga aagtaaatcc cggtatagaa agaataggaa caatacgtct tgtgaataat
      121 aacgatatgc atttctttcc ctattggctg tctgctttct atgcatccaa tgtgtacgga
      181 caaacggtaa tgaatatacc cgagagcgaa agagaagcac aataccggat agatatgaaa
      241 aaaatatata tactggaagt aaacggaata cccgtttcca tggcgggata taacagggaa
      301 ctgccgggat ccataggtgt ggcttatgtt tatacaccac cgtatttcag gaataaagga
      361 tatgcaagtt ccgctgtggc tcaggtcagt cagctggctt tagacaaagg gtataaaagg
      421 tgtgtattgt atacagattt atccaatccc gtatccaaca gtatttacgg taagatcggg
      481 tatgtacctg tatgcgattc attaatgctg cagttcaata attagagacg gaaacaataa
      541 atatttggtt ggaaatcgga agagatgaaa acaaaactgc aaaagcaatc ggataaactg
      601 cttaaggttt agataaccga caaatatctt caaagtgttc tataactgaa aatcgcattg
      661 attatacttg cagtaatggt ataatgactt tgtcaatttt ttatcactaa taggaggaaa
      721 gaatgaaaaa aagaataata aagggaatta tactgtccgt gctgtgtctt atattgcttg
      781 catcgctgac atcgtgtgca atggggctta gcggaacata caagaaaggt accgatttgg
      841 catacgaaac ctacagattc agcggtaata aattttacta tgaagtgggt gcactgggtg
      901 taaaagtagg cggtcacgaa ggcacgtata agataaaaga cgggaagatt acttttatct
      961 atgacgacag tgagcatagt tataaatttg agaaaggcaa agactatata ataatcaacg
     1021 gtgcttccta tgacaagaag tagggaagag gagtaattga tttaaacaga atacaaaagt
     1081 aaattcagtt aacaaaaaaa gagcttttat aagctctttt ttgtaagcag attagtctta
     1141 aaatcttctt ctgcaatctc catttcctca gtaatctatc cgattgtcgg caagtgcttt
     1201 ccgatacaat ttgccgctta agtgcgtagc cgttttctcc ttgtataact taaatcttaa
     1261 acttacccat taactctccc attaacttac ccatagcata tcaaatgaac ggttaaaggc
     1321 aatacttttg atatcattaa aaacctgatt tttagtatat agtacatatt attgtatttt
     1381 ttgttcagaa tgaaatgctg acaaaaagag cttatataag ctctttttgc ggagggagtt
     1441 atcctttcgg atatgagctg ttctgtgtac tatttgtctt ctgtgacaag attaaagccc
     1501 attatcttga agaggtaaat aaattcttta gtgtagtttc cgaatacggt aactctgtgc
     1561 cattgaggca tccagttgtt aagtatttct tcggatttac aaagtgcggc taacttgctt
     1621 ctgcaaccgg catctcctcc gtaattgcct accgatactg ccgaatgtat acatgcagtc
     1681 ttatcaacgt tatttatatt caccgttgtc agttgttcgt tggcggggaa tatgacttgt
     1741 accgcagctc cttttttatc ttcagcatga ctccttatat agtacttaca taacctttta
     1801 tcctgctctc cgaaaagctt tctgcaggag acgcaatgag cataaataat ctggtcggat
     1861 gatgtatcta taacgggatc ggatacaaaa ccgtatcttc cggttatggc ttttgtaaat
     1921 attgaggtta ttgtggaatc aatgtccgct tcgcatactc ctacataacc gtcttcgcac
     1981 atctggaaaa atgccaggca gggatacata tgtaagttgt tgccatatat atcgttatat
     2041 gacagactta aacagtctat tgttacggca tccgccttct tttctttgta catttccatg
     2101 attgcaagat agagttttgc actttccgaa acatcctgat ttgtcgcttc caatacacct
     2161 tcggattgtg aaatccattt atcttttata acttcagcat caatgtcctt gatattctta
     2221 taacgcttca tcaattcttc ggaattacaa ttgataaagg tgcagcccca tattttttca
     2281 gcagcagctt gtgtctcttt atcaatattg ttactaacca ctaatattct tgattgttta
     2341 accttcttta ttccgacaaa tactcttacg gcttttgcta tatcattaaa gtttgcagat
     2401 gcaataaccg gaacgggaag gtgttcttca cgtaccgtgt gtatagatct taccaacacc
     2461 gaaccgctac cgcaaaaagg tatatccgct ataatgcacg gtaacccttc ttttgctttt
     2521 cttgcataaa aaacatctaa attaatccag ttggtcataa gtaaaacaag aacgccgtca
     2581 tactttttta aatcatcggg ataggcagtt tccgcttgtg atacatgcgt atacttgaat
     2641 acatcaaact ccatatctgt attttgggag agtacattaa gaaccgactg tgtctctttt
     2701 tcgtaatcat agttaatatg cggccatccc ggaacatctt tcttttttgt ctgtacaacc
     2761 acttttatct ttgttttcat tttccacctc atagattatt cattctcgga aatgcaactg
     2821 atatcctctt tatttaatca ctttaatatc ccgtatccta ttcacttata cggctgatta
     2881 ttgtattatt acgtcataaa tactttttag gggcttttgc aatgttggat atcgaaaatt
     2941 atgatataag tataaattcc tgtgcgttta caaaatgtga tgaacattgg aactgggtta
     3001 ccgcaaaaca gggattcaaa gattatgacc tttggtttat aacgggggga aagggatata
     3061 tagaatacct cggtaataaa tatgaactgc atgaaggaaa atgtttttta ctggctccca
     3121 atgttcaata tgtcgcatat catgatatag ccgatatgtt atccgttata catatacatt
     3181 ttgattttgt taagaacgga atgaaagcat atcctttcgg agtaattgat tctcataccg
     3241 gtagcacaaa ccttattatc cggtcattgg agtatgttat gtttttatac aacatcaaag
     3301 attatcaaag agcatatatc tggtttaaag cggtattgtg cgatctgttc ggagaatgtg
     3361 catcagcaaa tgacggtatc gataaaggta ttcatgaagt catctactca atatgcaggg
     3421 taataggcaa ctcggtaaac atacccaatc ttgcggagtt tgcagagcaa tacagttatt
     3481 cggtttctta cttaggtaag atgtttacta agattaccgg tatgtcctac acagattatg
     3541 tcaatcacac aaaattgaac aaggcaaagg tattgctctg ttccacaaac aaaagtctta
     3601 gccggattgc cgaaaaaacc ggctactacg atgccagtta cttttccagg cagttcaaaa
     3661 agagtaccgg aatatcaccc ggtaagttca gaaaaaacag taccggattg gagtaattgt
     3721 ttataaatat tgaagtatgc tatatgactt atgcttaagt atatttttcg accggttgct
     3781 aaaaaactgc tttgaattaa taactcgtaa ctatgttgag acaatctgtt taaattatct
     3841 tctatgctta agcttaattt tttctccgtc attctcgaat atggaatact tattccttgc
     3901 tttcctggac caaaattttt ctatattaaa actatgtttc accaaacaat cagtaacatt
     3961 attgcattta caaaagtctc ttttaaaatc cttattggat tttgattgta ataaatcttt
     4021 aatttcactt atatcagttg catattttat ttcatcttca aggttgttaa cttgcggtat
     4081 aaggataacg tcctttacat gggaagattt ctttaatttc ccagtattgt cctttagcct
     4141 ttcgaccaat ttcatatcat caacatccgt atcgaaaaca ataatcaata ttgtaaaagg
     4201 cttaattgtt ttaataaaac cggaaggaat atctttttcc agcaaattga ttttagtaat
     4261 ccttcccgat tgaatgtatt tctgtcttat tgcgcgaatt aaacatgctt ccgtttctcc
     4321 ctctacaaaa tagttatagt ttatgttact catcgcaaaa ctctcctaaa tcccataact
     4381 gacttaagtc gggtgtagtt ccgaatacat catttacaaa agcatttctg atggaaatat
     4441 cgtttttctt tataaagtca ctcggatata cagcttctat attttcatcc ttttttaaga
     4501 aagtaaaaga atgcaccggt aaatccatgt ccagtagatc aaggttatga gaagtaaaaa
     4561 acagttgttc aagggggccc agtttgtcaa tcattattcc cagtagaaat tgctctatat
     4621 cggtctgtat aaaagtaaac ttctcatcaa cataataaaa accgtgcatg cctttgcata
     4681 ttgaacttat cacataagat atatctattc ccatttttgt tccgctggat aaaatatctt
     4741 caattatttt cccgttttga ataaaaacat ccccgttacg acttcttata acataagaat
     4801 tttctacttc atcgcttttt tgaacatcgg ttattgaagt atccaaagtt tttaagactc
     4861 gtttaagtat atccaatgaa agcacatctt tatcaatcct tatatcggtg ttaccgatat
     4921 cgggaaaagt aaaatgccat ccgaagcttt taatagaatc caatacatcc aatgaactct
     4981 tgttatcgtc ttttacttta acctgttcca actttcttac acaaatctca tatgaatctc
     5041 tcctgcctat aggggcagtg taaacttcca atgacagttt cggttccgct tctttcttgt
     5101 cgctgcgttc taaactgcaa ttaacgcggt acagcatttc ctcatcacgt aaaaaatcga
     5161 ttgaaaaaaa tgctttcctt tttttgtctt ttactgattc aaaaagtcta ccggggcttt
     5221 tatatgcgat aaaattaaat atatgcatca tggctttacc taatgtagtt ttacctgaag
     5281 cattcacacc gaggagaatg tttacttttt tgaaccgaaa attagttttg ttcggtaaaa
     5341 attcaccttt aattgtggaa tttacaattt tacgcggata ggaaaaatta atcttaaaat
     5401 catcgaaacc cagtaaatta tctaactcaa gattcattac taccataaaa ttgacctcct
     5461 gcatgatata agttccaatt attagaactt atatcatttt aatactgttt ttttcctttg
     5521 tcaagttttt aatctatcgt ttttgttact attcactgcc attgcaccgc accccaaaag
     5581 ggtaacactt ttcaacgaaa aattacgcac ataaaaaaca accaagattt atcctgattg
     5641 tcttgcttat tttgaattac ctgaaatatt tgacttccac ttcgctttac ttttgaacgc
     5701 atttttgcta tcatactttc agataacaga taggagaaag cctatgttct tctccatctg
     5761 ttccgaaaga aaacacagaa aactcctcgc cgggagattt aaaaagctaa gacaaaacaa
     5821 tcggattgca gaaaagtggt gacaaactat gactacttgg gcagattata aaaataatgt
     5881 aagagaaact aatcctgaaa tcggaaaaga tattgacgaa gttgaagcga tttcaaaaat
     5941 tgtcggtgct atgattgagc gccgccatga ccttgaactt tctcaaagag aacttgccga
     6001 tatgtgcggc ataccgcatt cttccgttgc cagaattgaa tccgggagca gtactccgaa
     6061 tcttcggaca cttttgaaaa tattccgaga actcggttta aatttagttg cagaacccgc
     6121 agtaggttct tctcctaacg atgtgagcct gtaataatat tgcaaaatta tttaaccaaa
     6181 catggaattt gctaataata agtttttctg ctttttcata gcatttatta aaaaactgtt
     6241 gacaacagga ataacaggtg gtattcttta acaggttagc gactgctaac catgtgtgaa
     6301 agagggtgct atgatgccgt tgatatcagt taagaacggg gaaaaagcgg ttataaaaaa
     6361 ggttaccgga agtaatgaag taaggaagta tttaaccgac ttgggattcg ttgaggggtc
     6421 ggatatttgc gttattacgg aaataatggg taatatgatt gtaaacataa aagaatgcag
     6481 aattgcctta aacagggaat tggcttcgaa aataatggtg gaggtttaaa gaaatatgga
     6541 aacattggat aaggtaaagg cgggacaaag cgttactgtc agaaaaatac gcggcacggg
     6601 cccggtaaag aagcgactga tggatatggg gataactcag gggacagaaa taacggtagt
     6661 aaaattggca ccgttgggtg atccgattga aatgaagtta agaggatatg atctttctat
     6721 cagaaaagcc gatgccgagc tggtagaggt ggaatagtaa atttttttaa cataaggtta
     6781 gcaaacgcta actaaaggag ggaaaagatg aaaatagcga tagcaggtaa tcccaacagc
     6841 ggtaaaacaa ctttattcaa tgcgcttacg ggattaaatc agtttgtggg taactggccc
     6901 ggagttaccg tagagaaaaa agaaggtaag ctgaaaaaga agaaaaatgt acacattatg
     6961 gatcttccgg gaatatattc actgtcacct tattcattgg aagaaataat tgcaagagat
     7021 tatctggtca atgaaagacc cgatgtgata attaatatta ttgacggaac caatctggag
     7081 cgctcattgt atcttacaac tcagttggca gaactgggca tacccatggt catagccgtc
     7141 aatatgatgg attctgtgga taaggcggga gataagctgg atacgaaaaa attgtcggaa
     7201 cagttaggtt atcctgttgt agggatatcc gcactgaaaa agcgtaatat tcagcaactg
     7261 ataaaggaaa ccttaaaagc cggggaagat aaagggtccg tacccgttat gaagttcaac
     7321 agtgtagcgg aagaggctat tacaggtatt aaagagattg taggtagtaa agccgacggc
     7381 ataaatgaca gatggctggc agtaaaattc tttgaaagag atgaaaaagt atccgatacg
     7441 tataaattga acaaaagtga agcggaagca gtagaaaagt atatcaaaaa gtgtgaagag
     7501 aaactggatg acacctcgga aagtataatt gcaaatgaaa gatataaata tgcggcaaaa
     7561 gttgcatctt ccagttttgt aaagaaggat aaaggggcac ttacggtaac cgataagata
     7621 gataaagtgg ttaccaacag atttcttgcc ctccccatat ttgtagttgt tatgtttatc
     7681 gtgtacttcg tatcggttac aaccgtaggc gcaattgtta ccgactggac caacgaagtg
     7741 ttattcgctg aactgatagc tccgggagtg gaagattggc ttgtaagtat aaataccgca
     7801 gactggctga caggccttat agtagacggt attatagggg gtgtaggtgc ggtattggga
     7861 tttgttcccc agatgtttgt actgttctta ttccttgctt ttttggaagc ttgcggatat
     7921 atggcaagaa ttgcctttat actggacaga ttattcagaa aattcggctt gtccggtaag
     7981 tctttcatac ccatgcttat aggtacgggc tgttcggtac cgggtataat ggcaagcaga
     8041 accattgaga atgaaagaga cagaaagatg acgattataa caagcatttt catgccgtgc
     8101 ggtgccaaac tccccataat cgcccttata gccggtgctt tgttcaacga ggcatggtgg
     8161 gtagctccca gtgcatattt tatgggaata gcagcggtaa taatctccgg tataatactt
     8221 aaaaagacca agccgtttgc aggagatcct tcacctttcg taatggaact tccggcatat
     8281 cgttggccta cagtgggaaa tgttctgaaa tccatgtggg aaagaggctg gtcctttatt
     8341 aaaaaggccg gtaccgtagt actgttatcc agcatattca tatggtttac ctcttcattc
     8401 ggtattattg aaggtaaatt tacaatggtg gaagaccttt ccgacggttt tctttccaag
     8461 ataggtacag ccttggcatg gatatttaag cctttaggat tcggtgaatg gagattaacc
     8521 gtagctacca ttatgggatt ggtagcaaaa gaaaatgtag tatccacaat cggaatactt
     8581 tacggatttg ccgaagtttc cgaaagcgga gaagaaatat gggagctttt agcagcttca
     8641 tatacacaaa tagccgccta ctcatacttg gcattcaacc ttctgtgtgc tccctgtttt
     8701 gcggcaatcg gtgctacaaa aagagagatg aacagcggca aatggacatg gtttgcagta
     8761 ggatatcaaa ccgtatttgc ttatgcggta gccttgatta tttatcagat aggaacattg
     8821 atatccacag gcgcattcgg tttatggacc gtaatagcat ttattgtatt aggtttaatg
     8881 ctgtacttac tgttcaggcc tcaacctaaa agaagtttaa cgggtgaaag gataacggca
     8941 tgaaagagtt tatattagaa aaccttgcaa ccgttattat ctcccttgta ctgttatcgg
     9001 ttgtaacctt aattgttgta agtatgataa gaaacaaaaa gaaaaacaag agcatatgtg
     9061 caggttgccc catggcgggt aactgctcaa aaggtaaagg ataatattct ccgatatagt
     9121 aaacatgtac aatcctaaga caatctcctt gcggaggttg ttttagtaag aaaatttttt
     9181 tacttttaga tgataatatg atagttttaa gaagtgaata tgcatgttta ggagaaagac
     9241 attaaaaatt attgcaatgt agggaacatt atagtaatat ttaatcataa aatgttccct
     9301 atggaggcgt tatgaacaat aactattcaa gtatacaaaa tttacttcat caaaaagcgg
     9361 attgccaggc aagactgaat cttttacctt atgacggaaa tcccgagata aaagaacgtg
     9421 acggaaggaa gtatttgtat gtacgcaaac gagtgggttc aagactttcc tcaacctatg
     9481 cgggtattta ttccgatgag ctgtatcagc ttcttgttcg caatgcccgt gaagcaaggg
     9541 agatacgaag aaatatccga agactggaca aagagctggc gctgctcgga tattccgaca
     9601 gagggcttac acctaaagtt cagcttaatt tggattttgc cagaataaac attaaatcca
     9661 gtatctttga tcaggcaata cttgaagggg ttgcaacaag tttttctcag gttgaggata
     9721 taattgaaga taatactgtc agcggtatga ctgcaagcga tgtgcaaaag atattgaatc
     9781 tgaaacatgc ctgggagttt attcttgata aggatgtaat agcttatccc tcggattatt
     9841 ctattctttg ccatatagcc aaacttgtta atgagggatt ctttcaggat ggcggacgta
     9901 ttcgcggtgt tcctgtaaca atagggggct caaactatat tcctcccatg ccgatcgaat
     9961 cggttgttaa ggaaaatata caggctatat tgcaagcgga tataccgtcc gatgaaaagg
    10021 caataaaact ttgtctttat tgcatgaaag cacagatatt caatgacggt aataaaagag
    10081 cggctattat ctttgcaaac cattatctca tatcccaagg cggcggaatt attatagtgc
    10141 cggaaaagaa tgttccccaa tttaagacat tacttattgc ctactatgaa gacagagata
    10201 aaggtgagat taccgaattc atgaaaaagc agtgtattaa aaccttctaa tcttaagcgg
    10261 gcattcttaa tttaaaaggt atacaaaaac aacttatatt catgtcagtg cagagtatgc
    10321 attttttatc acttaaagaa taccgaaatg caacgaatag catatatttg ctctaaaagt
    10381 gcaacgcaaa tgcaacgaat aattatttgt tgcatatttg ttgcattatt tatataatac
    10441 ttcatagaaa gtgtcgcatg aaagaaggta agataatgga aggttccaat ttgttttatg
    10501 atcaattact gtccggtgat gagcagctta gttttacata tcttgaaaag aaattaaggg
    10561 aaattattaa aattcccgcc ctggataatg atatactgcg ttctttaggt ttttatacaa
    10621 aagatatgaa gtataacaat gcagcttcaa tatttgcaga taagaataat atgttcggtg
    10681 tggatatcgt ccgtttcggc aattcaatta atgagattct tgacagagaa acaatttata
    10741 aaaactgcat tctggaacag tatgacagag cattggtatt gtacagaaaa tattatcaat
    10801 atgaagtgat tgagggagca ttacgtgaaa gacgtgaacg acttcccgaa tccgcattca
    10861 gggaagcact tgcaaatgct ttggtacaca gagattttga tgtaaatgca catataagga
    10921 tagcaatgtt taagaataaa atagagattt cttcccccgg aggtcttcct tcttcaatta
    10981 aaaaggaaga ttatctgaac ggcactatct ctgttttaaa gaatcctata attggtaata
    11041 tctttttcag attggattta attgaaactc tgggaacggg aataaggcgc ataaaccagg
    11101 catacagaga ttatgatata aagccggatt atatgatatt tgataccagt attacggttg
    11161 aacttccgac aactaccgta gagtacgaga ttaccggcga agaatcggat attgttgatg
    11221 ctctgagagg cggtaggatt ttaacatgta gcgaaatcac ggataagacg gggtataata
    11281 aaagcaaggt tctgaggtta atgaaaaatt taaccgataa gaaatatgtc tccgttatcg
    11341 gtggcggaag aagtacaaag tataaactga gataagtatt taaaaggatg ataataaaaa
    11401 gacagcctct tttgaaaggc tgtcttttcg agaagtatga gattgctttt taagcagaca
    11461 atcatttacc caatccgaac agatttaaga agcgctgcca gaaggtaatt ttagcctgtc
    11521 cgttagtagg tgttttggct tctttttcca attctattgc tttggtcttt attataaact
    11581 taaccgattc taccgattta ttcttgtcgg agacataaga tttcactata tccgcatctc
    11641 cgaatatctc gtctatcata ttgtctattt cttttcttat cttggaatcc agtcccatgg
    11701 tttcgtaatg caaggttctt acaccgtctc tgtacttaag gagaccttcc ttcaactcga
    11761 ccgaagcact ctggagggtg gctgccgatt cggtgagagt atccgcatat tgttttattg
    11821 tagccgccga tgtgtttatt ttgcatattc cgtttttaag ttccgttgcg gcctgactta
    11881 cttgagtaac cgatccggtg tacatatcca ttccggcttt cagttgtata acaccgtcca
    11941 gttcggcttt taaaggtgcc agttccggta cattctccag tattgcggca tagttctgct
    12001 cgttaagttc gggaagaggt aaacccatct gctgtatcgt gccgtttgcc gtctggaatg
    12061 ttttaagtac gataccgtgt atagcctgtg ctaaaggtgc tccctgtcgg cttaatgcat
    12121 ccagcccgcc tgccagttgc gatgctccct gatatacctg actgataccc tgtgataact
    12181 gtgctatacc gtctctgaag cttgcctgtg cctccgtgta ctgtgttaaa cccgatacat
    12241 attcttcgaa accgtcggta agttcctcag ctccgtcatc taatttcttt atggcatcgg
    12301 taagctcgtt gaatctttcg gttatggaat gggtatccac cggcatatcc ataatcattc
    12361 ttacaccgcc gaaggtaacg gaatccaatt caaagttctt tgcgtcggct ttaatattaa
    12421 tgtcggcatt tttgccgggc agtaccgtat aggttatctg tttgtacata ccggctgtgg
    12481 caataacgcc gttctgtgtc tgtatatcgc tgaatattct ttcgtccaac tttacagtta
    12541 ttgataacgt gtaattgctg taaaagaact tatcgctttt tgtatcgggc ttcaccgata
    12601 tccttatttc cgcttttccg ctctttccga ctatttcgtc tgctttaagc tctttaccgt
    12661 ccatcagata cttaacctct attatccagg gtaagttttt ggtatccaat gtaccctgat
    12721 aatgcagttt ttgtgctttg gaatctattg ttatcaagtc gtcttgcacg gttactttat
    12781 cgtttgtggt taagttctta accgaatcgt atgtaccgta atcggtaatg cttccctgat
    12841 taaaggaatt aataacataa acttgttgaa tttgtccgta ttcgtccagt atgccgtaaa
    12901 ttacttcttc tttttttgaa gattccggtg ctgccgatac cggtaatatc gaagccgcta
    12961 aaagggctat tataagaagg atactaatca gttttttcat ttaatgtcac ctcctgtttt
    13021 tttacttaaa tgaaatccgt ttttataggt tgtttttctt attactttat caaacagtat
    13081 caacagagcg ggaagtaccg tggataccat tacaaaggac aatatagttc ctcttgcaag
    13141 caagcctccg agctccctta caataggatt ggatgatgtc agtgtcagtg caaatccggc
    13201 agaggccagt atacccgccg atatcattac cgcaactata ttgtttgcca gtgtcttggc
    13261 catagcctct tttgcgggta gcaaggtacg attgtgcata tacctgtcgg tgataagtat
    13321 cgcatagtca atggtagctc ccagctgcac ggtattaatt acaaggtaac ctatatagtt
    13381 taaagagctg ccttggaaat atgtgaatgc caggtttatc catattgccg cctcaatggt
    13441 aaagaccaat ataaagggca gggataacga tttgaaagat ataagtatga caaggaatat
    13501 gcccaatacc gcaatgagtg tgacaagggt attgtcagcc gttattgaat cctttatgtc
    13561 atacaccgat gcactttctc ccactaagta gacctcgtcg taatactgct ctgcggtagc
    13621 gcgaatcttc tctatggctg caaaagtttc tttactttct atttcatagt cggtatacag
    13681 tattattctg gaatagtttt ccgaattgaa catactgtcc tgtccttcca caaaaccttc
    13741 cggaatagcc gggcctatat agcttgcgta ggaaattaca gcagaaattc cctcgatatc
    13801 ctccagttca ttactcagca tattcaacga accgttaccg cctttaggaa ctattatcgc
    13861 catctgatta atctttccga attgctcctc tatagccaat ttgtcctctt ccgctctgga
    13921 tgcctttata ccggcgccgg agccgtattc aaatggaatc ttatcctttg caagaaaaca
    13981 ggggataata accaaaatgg atattattaa aagaggtatt ctcagcttca tcagccactg
    14041 tcccaccctc tttaaatcca tacggacggt tttgtgttgt gtcttatcca ttatgcgcac
    14101 gaaagaaaga gccagtgcgg gcaggaatat cattacactt ataaagctca acgcaacccc
    14161 cttaactaag ttgataccca aatccgcacc tatctggaat ctcatgaata acaaagccaa
    14221 aaaaccgata accgtggtgg ctgcactggc agctaccgaa gtaaaggatt tctttattgc
    14281 gcgtttcata gcttcttccg gttcatactg ttcccggtat tctttaaagc tgtgcattaa
    14341 aaatattgca tagtccagtg agaccgccat ctgcaaaatg ggactgacgg tgtaagtaat
    14401 aaaggaaatg tcgtcaaata acagattggt gcccatgtta atcaatattg cgacacccaa
    14461 tgccgcaaga tagagcagag gctctatata tgacgatgtg gtcagaagta aaacaatgat
    14521 aatgagagga accagtatga gcagcgacct caatgtttcg gtagccgaaa gcttttgtac
    14581 aaccgcttta ctaaccgaag agcccgatgc cgcattttct tctcctatca actcatatat
    14641 accgtctacc gcttccacct cgaaaccggg agctatggaa acggaaaaca gggcattgcc
    14701 gtttttgtag tatgcttccg ttaccgaact gtccagaaac tccaaaggtg tggaatgtaa
    14761 gatatcctgt cctatcacat catccagcca tgtaaccgca gtgacaccgg atatattttc
    14821 caaatcctct ttatactcca acgcctgatg aaccgataca ttccttacca taatacttgc
    14881 agtagggaag tcctcatcaa attctttttc cattaaactc ataccttcgg tggaattact
    14941 gtccttggga agatagtctt ccagattata gtttaccggg acacccatcg ccattacccc
    15001 gcataacacc gcaagagtaa tgaatatgat tattatgagt tttcttcgtt taacaatacc
    15061 gcttgtaaat ttttccataa cactccttcc aataaacaac attgtgttga caaatcacca
    15121 ctgttatagg ataatatcat tgggttgata agtcaacaga cagatgttta ataaatgttt
    15181 gttgacaaac agaaaagagg gaaatgtggt ttatcagaca aaaacggggg agatgattat
    15241 gaagaagcaa aagaaagaag acagaagggt gaagtacacc aaaatggtgt taaaggaaag
    15301 tcttattgac cttttatcac agaaggatat atcggcaatc accattaaag agatatgcga
    15361 agaggcggac gtaaacagaa cgacatttta tgctcattac gccgatcagt atgatttgtt
    15421 gcgacagatt gaagatgagt tactgcaaaa tctgaaggca tatataacgg tattcaaaca
    15481 gaaaaaagcc gatgccatat ttgtggatat tctggaagaa atgtttgaat acataaaggc
    15541 caatgcgaaa ctgtgtaaac tgctgttatc caaagagggc aatctggact tccagaaacg
    15601 tattatgatg ctggtatacg ataccaatct tgtggcaaaa cccaatgaga ctctgaaaaa
    15661 gggagaagag gaatatgtat actcctttgt cataacgggg tgtgtgggag ttatacataa
    15721 gtggctggaa gagggaatga aacactcatc ccgtataatg gcgggaatga ttatcaattc
    15781 ggtaaagaat ctgcctgtga catttgagta aatgagtcac aaaacttttc agctgaaggc
    15841 ggctattttt tccttatgta tataagcttg atattattca ggtttacgga ctttatgtcg
    15901 aattttttca gcgacgacac aaacggcgtg agtttgctga atccgtagtt tcgtacatca
    15961 aaatcgggaa acattttgga cagtatattg cccacatcgc ttaacgggct ccaaccgtca
    16021 tcatcggata tatcatcggc tattgtttca atagctttta ttaccttctc cagaggagta
    16081 atggaatcct ccatgtcttt ttgcttgtta ttgttctctt cctgacccag tatctccaag
    16141 tatttgaatt ggttgcagga tgctatgaag ggggcggggg ttttcttttc tcccatacct
    16201 acaacatatt tacccgcttc acgaagcctg gttgccagtc ttgtaaagtc cgaatcggaa
    16261 gaaactatac agaatccttc aacattatcg tcatacagta tatccatggc atctataatc
    16321 attgccgaat cggtggcatt tttacctgtc gtatatccgt actgctgtat aggtgtaatg
    16381 gaatagtcta agagtatttt tttccaggaa ctcagttccg gtctggtcca gtctccgtat
    16441 atccttttat aggttatcgt tcccgatctt gccagttcat cgaatatata ttttatatat
    16501 ttttccgata cattctcggc atctataagt actgcaaatc gcttatcttc attcatattt
    16561 ttttacctca ttatatataa tatcataaca tccgtttttt ccgttatttg tgtcttgatt
    16621 gggttatttc ctctttgtat atcttatctc tgtgtcctat atcgataagt accagtataa
    16681 gtatattatc ccgtatatcc gctaaaatac gatacttacc tactcggtac ctccaataac
    16741 cggttttttc cgatgtaagt cctttgccgt gtatgcgggg attttcacaa ttaaccaggt
    16801 ttttatcaat ccaggattgg ataatctttt gtgtctgtct gtcaagcttt ttgaaagctt
    16861 tatcgaattt ctcagtagtg attatactgt aagtcataat tgcaactctt cccaaagctt
    16921 attaacaggt cgagttttca atttaccttc cgatttctct ttttcgtatt cggcaaatac
    16981 ctgcatatca aattggtctt caatatgctc catcatgaca tttctgacaa cttccgataa
    17041 actcttgcca tttatttttg cataagcttt cagaatgtca agttcctcat cggttatccg
    17101 caaactgatg gttgccataa tgcacctcct cgtgtccggg tttattgtaa tacgattgta
    17161 ttacatgtat gtccctttgt caacaactac cgatagcatc tttcatcagc agtaccatta
    17221 ttgccttttg tgcatgaata cggttttccg cctgctcgaa tatttccttt gcatgttgtt
    17281 caaatacctc ttcggttatt tcctgctccc tgtgagcggg aaggcagtgt aatactatgg
    17341 catcgggttt ggcagaagct gttactttac ggtttatctg atatcctttg aatgcttccg
    17401 ttcttatctg tacttccttt tcatgaccca tggatgccca gacatcggta tacaatacat
    17461 cggcatcttt tgcggcttca aaaacatcgg ttgtacataa aaagtcggga ttcggtgctg
    17521 cccattgcat tactgtattg tgcggctcat aaccttcagg gcaggctaca ctaattctca
    17581 ttcccgtctt caaagcgccc gctatcaaag agttggccat attgttgccg tctcctatat
    17641 agcacatttt aagtcggcaa gtgttccttt atactccctt attgtcataa ggtcagccaa
    17701 cacctggcag ggatgttcaa aatccgtaag tccgttaatt accggtatgg ttgcatattt
    17761 tgcaagcttt tccacttctt cctgtgcgaa agttctaatc attatacagt caaggtatct
    17821 cgacagtact cttgcggtat cctgtatagg ttcgcctctt cctatctgaa ggtcattgct
    17881 gcttaaaaac agagcatgtc ctcccaattc atacatgcct gcttcaaaag atacccttgt
    17941 tctcgtggag gatttttcaa atatcatacc gagttttttg ccttccaagt attttttgtt
    18001 aaggttattc tttctttcat atttaagctg gtcggccaga ttcaatatct ttgttatttc
    18061 ctctttgcca agatccataa gtttcaacag atgtttcatt attcacatgc ctccttcagc
    18121 acttttacgg cttttgccag cgatttttta catatattca acggcggcag catacgtatc
    18181 ttatcctttg ccgtaaggaa taatacgccg tttgccatac atttttccac tatttctttg
    18241 gcatcttttt cggttttaac cgcaagcatc aatcccgttc cgcttatcga ttgcacccct
    18301 tttgcgcctt gtaacagttc ccttactatt ttttcctttt caagtacatc ctttaaaagt
    18361 ttgttgttta ttctgcttac gacacttatt gctccggcac agcaaacggg gttacctccg
    18421 aaagtggagc cgtgagagcc gtagtccaga acattgtgtg ttttctcgtt gaacagggta
    18481 gcacctatgg gtaaacctcc gcccaatccc tttgccgaag tcactatatc cggttgtatt
    18541 tcgtaacgca tatatgagta aaatgcaccg gttctgccta tacctgtctg aacttcatcg
    18601 ataatcaaaa gtacatcatt ctctttacac agtttttccg tattagcaac ataatcttta
    18661 tccagcggta taacaccgcc ttctccctgt ataagttcca gcattacggc acagacatgt
    18721 cctgatttga tatgcttttc catctctttt gtatcattca cttttacgtg taggaatcct
    18781 tgagtaaaag gtccgaaatg cttatggaaa acatcctgtc ccgttgcgga taatgtagtg
    18841 acggttcttc cgtgaaaact gttttccaat gtgattatcg tatgtctgtc ctgtccgtat
    18901 ttgtcggaac tgtattttct tgccgttttg atggcacact cgtttgcttc ggcgccggaa
    18961 ttggcaaaga atactttact cataccggac tttttgcaca gaagctttgc cagttccacc
    19021 tgcgggtagg tgtaatagag attggatgtg tgctgcagtt tatccagctg agcgattatt
    19081 gcctgtttcc attgctcatc cgcaacaccg aaactgttta ccgctatgcc gcttcccata
    19141 tctatgtatt gcttaccgtt ggaatcataa agaaattgct tgtgtcctct tacaaggtcc
    19201 aaatcaaacc ttttgtatgt attggctata tattgcttgt ccagtgcttt aatatccatt
    19261 acccgcctca cgcaaacatt gttcctatac cttcatcggt aagtgtttct ataagtatgg
    19321 agtgggggat tcttccgtct attatgaata ccctttttac gccccttctt actgcttcta
    19381 tacagcattg tactttggga atcatgccgc cctcaattgc accttcccgt atcagcctgc
    19441 ttgcatcgga taccgagatt ttggatatta atgatttctg atcgttctta tccgacaaaa
    19501 gacccggtgt gtcggtcatg gagataaggc attcggcatt taaggccgat gctatagccg
    19561 aagatgccgt gtctgcattt atattatagg tattcatgtc gtcatcgcag cctaccgtgg
    19621 atattacggg aatatatccc ttttccagaa ggtcctcaat aaccgatttg tttatgtcgg
    19681 ttatctctcc tacaaaaccc aattgctcac taatcttcct ggcctttatc aagcttccgt
    19741 cgtgtccgca tatacctatt gcttttccgc cgtatgattg cagcatggat accagattct
    19801 tgtttatcct gccgcacagc accatctgta cgatatcggc ggtttcccgg tcggtcactc
    19861 tcagtccgtt tacaaactcg gttttcttgc ctatcttgtt gagcattccg gttatttccg
    19921 gacctccgcc gtgtaccagt accactttca tgcctatgga agacatgagt acaatatctc
    19981 tcattacatc tttttttaga tgctcttcgg tcattgcggc accgccgtat ttgactacca
    20041 caatcttgtt gttgtatttc tgtatatacg gcagggcttc taccagtata tgtgcccttt
    20101 gggcattgga aagtgtcatg tcaattctcc ttttaagtac ggtaatctcc gtttatcttt
    20161 acatagtcat atgtaaggtc acatccgtat gccgtcgcct gagcatctcc cgagttcaga
    20221 tttattaata cggttatttc ttcatagctc aatattgttt ttgcaagttg ttcggaaaac
    20281 tgcactccga atcctttttc gcagacttta acggtagttt ccttgtcatt tgaaaaagaa
    20341 acatcaatct tacctatatc cacatccgcc ccggaatagc ctattgcaca aagtatccgt
    20401 ccccaattgg catcggctcc gaacatggcg gcttttacta aagaggaaga tattaccgat
    20461 ttggcaacgg ttttacatat atcctttgtt ttgccgttta ttaccttaca ttcaataagt
    20521 ttggttgcac cttctccgtc cttgactatc atgcggctta aatacgatgt tacggcattc
    20581 aaggcttttt taaatatttc atattctttg tttttttctt ttatttcact gttttttgcc
    20641 tgaccgtttg caagtaccga taccatatcg tttgtggaag tatcgccgtc aatacttatc
    20701 atgttgaatg tatcttttac atcttccgac aatgcttttt gcagcagttc ttttgatata
    20761 ttcacatcgg tggttataaa tgccagcatt gtggccatat cgggatgaat catgccggat
    20821 cctttggcaa tgcctcccat cttgcaggtt tttccgttta tcttaaaact taccgccact
    20881 tccttaaccc ttgtatcggt ggtcattatg gcttcggtgc agtgtatgcc gttttcttta
    20941 ctcaatccgt cacacagctc tttcatatgc gaagcaatag ggtctaatga cagtttctgc
    21001 cctataaccc cggtggatgc cactattaca tcggcaggat taatgtttaa ctcctttgcc
    21061 gcgagattac acatgccccg tgctatctcc ataccgtttt cattgcaggt attggcattg
    21121 ccgctgttgc atatgatggc ttttgcatat ccgtccgcca cattctgttg tgtgaccgtt
    21181 attggtgctc cctttacaag attttttgta tagacgcaag ctgcggtaca tatagtatcg
    21241 gaaacaataa gggcgagatc ccttttggtc ttattcttcc ttataccgca gtgtataccg
    21301 tttgctctga acccgacggg tgcggttatt gaacctttta tatattccat tctgttattt
    21361 ctcctttata ccttcaaacc gtaagtttct tcttttccgg ttatgacatt caatatctgt
    21421 accgcc
//
LOCUS       DAOSOM010000032        21243 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_2484_length_21243_cov_4.951860, whole genome shotgun sequence.
ACCESSION   DAOSOM010000032 DAOSOM010000000
VERSION     DAOSOM010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 21243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21243
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2484_length_21243_cov_4.951860"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..1191
                     /gene="tgt"
                     /locus_tag="PLF14_07560"
     CDS             70..1191
                     /gene="tgt"
                     /locus_tag="PLF14_07560"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOT71179.1"
                     /translation="MSRYPVTYEHIHTCKQSGARLGIVHTPHGSFETPVFMPVGTAAT
                     VKGMMPKDLKEIGAHIILSNTYHLYLRPSDQVVAEAGGLHKFMNWDRSILTDSGGFQV
                     FSLNTLRKITEEGVTFRSHIDGSSHLFTPEKVMSIQNNLGADIIMAFDECAPYPAERS
                     YIDDSLLRTARWLERCINSHNRSSEQALFGIIQGGMYKDLRIKSAKQITSFDLPGYAI
                     GGLSVGEPAEVMYEMLEHVTPLMPFDKPRYLMGVGTPDYLIEGSIRGIDMFDCVLPTR
                     IARNGTVLTSKGRVIVRDAKYSRDFTPLDDECDCYTCKHFTRAYLRHLFKSDEILALN
                     LATLHNLKFLLDLMAKIRQSIKEDKLLDFRDEFYEKYGY"
     gene            1215..1574
                     /gene="yajC"
                     /locus_tag="PLF14_07565"
     CDS             1215..1574
                     /gene="yajC"
                     /locus_tag="PLF14_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOT71180.1"
                     /translation="MFNLILAETAATAETGGRNWTSLILIVVVFVVMYAVILIPQRKK
                     DKKLKQMLADLKNGDVICTIGGIMGKIVNVQEDEITIETSIERTKMTMKKWAVRNVET
                     VEEESSDAEPTGEIESK"
     gene            1731..2450
                     /locus_tag="PLF14_07570"
     CDS             1731..2450
                     /locus_tag="PLF14_07570"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HOT71181.1"
                     /translation="MDEIRGFLIIGVVISHTLFDMHYFFSMDMSWIKSPLFTAISDIG
                     AIIFILISGMVSEFSRDNINRGLKLMCVAATVTLVTHIVVPDYVVYAGILHLLSFAIV
                     LHELIKPLLVKINRIAGLVINILIALLTANLYGREISIFGVTLFKIPDNIMNGGLSYL
                     LGLKIDNITVSSDYYPILPWITFFFMGFYLTEYIERQPLKSFLCRKRCSFLAKAGRNS
                     LLVYLIHQPVVMGILYLISLI"
     gene            complement(2437..3705)
                     /locus_tag="PLF14_07575"
     CDS             complement(2437..3705)
                     /locus_tag="PLF14_07575"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HOT71182.1"
                     /translation="MKKLLSAIITVLIVTLLITLTPSTTTYCKADILREAISAASEDT
                     IMDYTSIDKWNKQNDSIYINTPLFKEDLVSKIKKISVIPEAKRYIKNTDGSYAEIFGD
                     YYKRLIDNIDYDSVKDTDTDNIRYGFVIKRSALKAFPTYDEVFSSPSSVHDRFRESTL
                     YPFEPVAILHQSKDKQWLFIKMYNYEGWINKADVAVTSEYIVKLYEKNRNFIVITRPI
                     AYLRYSHTKSDMGCVFPLVKEHDNYYTVLCPKQGADGTLYFSFDKVEKQSAHVGYLPY
                     TQKNILIQAKKFLNEPYGWGGMNGYRDCSALVMDIFRSMGIKLKRNTDQQETMTPSKT
                     NVKGMSKQEKEQLLKTQKAGSLIFMDGHVMLYLGIIQDKPYIIHDITGYYSEGSYINA
                     YKVTVTDVYIKNSKQEEYINLFTTFLFISN"
     gene            3799..4248
                     /gene="rplI"
                     /locus_tag="PLF14_07580"
     CDS             3799..4248
                     /gene="rplI"
                     /locus_tag="PLF14_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOT71183.1"
                     /translation="MKVILTQDVKNLGKKNQLVNVSDGYARNFLFKKNLAVEANAKNL
                     NIMKDRKQSESSKKDRELVEANLLQSRLEGKQVKMEVRAGESGKLFGSITAKDIADAI
                     AEQYKADIDRRKIQLDEPIKNTGDADVVIKIHPEVSINIRLVITEKV"
     gene            4250..5581
                     /gene="dnaB"
                     /locus_tag="PLF14_07585"
     CDS             4250..5581
                     /gene="dnaB"
                     /locus_tag="PLF14_07585"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOT71184.1"
                     /translation="MSKDVKRVPPHSQEAEQYVLGCLLLDSSAFEPVQVKIDSSMFYI
                     PAHRDIYNALQAVFLSGIPVDIVSVAEQMKKDDTFTDAGGMEYLNKLASQVVSASSAE
                     YYASIIFDKYILRSLISLSGQVFEDAYSDSKEAKELLEYNQQELFNLSEQADTKGVKK
                     ISDLVVRRLDYFRQVSKTKEIPNCIETGFADLDKLILGFRPGDFILIAARPSMGKSTL
                     AMNMAQNMAIRKGKNVLFLSLEMSNEQLTDRMIASEARVPNLTLQRGEADQNEWAKVA
                     ATAGIIHESRIFLDDTSSITVADIRSKCRRFKAAENLDIIFIDYLQLIGSAVKKENRQ
                     QEVTEISRQLKILAKDLKIPVVAMSQLSRASEQRADRVPQLSDLRDSGAIEQDADMVM
                     FIHRPQELEKNATTGIVNLIVAKNRSGSTGTVQLVWQGPIVRFDSYTKRDD"
     gene            5574..6950
                     /gene="tilS"
                     /locus_tag="PLF14_07590"
     CDS             5574..6950
                     /gene="tilS"
                     /locus_tag="PLF14_07590"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOT71185.1"
                     /translation="MIKLDLDDNLIKDKPWYIAFSGGPDSACLLYLLMKKREEIEKKY
                     KVSVKLSAIHVNHNLRQSESKRDEDFCRRLCEKYDIEFLPYDVDVNNYAKEEKLTVEQ
                     AARVLRYEIFDDYSYGCVFLGHNKDDNAETVFLNILRGSGVQGLCGMQKVSEHYFRPL
                     LDYTKKEILEFNRENGIEYVIDSTNRASYYMRNYIRNEIIPALNEKTNKDIVGNLIAL
                     ADIATQTQSYINKQIDAEWDKRVTAGKDHVLVDVKDFVDMDQVISSGLLRKAIRYVKK
                     GLKDVEKKHIEIVHTLILHSQSGAVVDLKDGIRVLLLQNNRVKIYRQSDEDYDFCVAV
                     NIEGDTFIESRNINVRASACIFEGGDEYTEQRVFLDFEAVKQGLVIRNRKSRDFIYPA
                     KGNGKKTLKKYFIDKKVDSDLRDRLFVLAIENEIVYIENKEIGKRFRPVKGSKALRLE
                     FVKYERTV"
     gene            6934..7479
                     /gene="hpt"
                     /locus_tag="PLF14_07595"
     CDS             6934..7479
                     /gene="hpt"
                     /locus_tag="PLF14_07595"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOT71186.1"
                     /translation="MNELYNDVSEIMITKERLDEICAELGKRISSDYKGKQLMLVGVM
                     KGGLIFLSDLLRKITIPVEFGTITASSYGKGTSSSGQVTILQDLDMDIAGKDVIIVED
                     LIDSGHTLRYLQEFLGLRNPNSVKICCMLDKPSRREKQVKVDYIGQEIPDKFVVGYGM
                     DFEEKYRNLADVCVLKKEFYE"
     gene            7562..9397
                     /gene="ftsH"
                     /locus_tag="PLF14_07600"
     CDS             7562..9397
                     /gene="ftsH"
                     /locus_tag="PLF14_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOT71187.1"
                     /translation="MKKYRNISFYVILFIIAVILISLFTGRDSVKELGYSEFLNQLEK
                     QNVSEVIIRTTTLTVTLKEPIKDGNKVFHVNVENLDKIEDILNEARLSDPDLSYTWKP
                     ETTSWWLSLLPSFIILAIVIIVFFVFMNKQAGANNSAMSFGKSRAREYSEKDRKVTFL
                     DVAGADEEKAELEEVVDFLKQPEKYKKLGARIPKGILIVGPPGTGKTLLARAVAGEAG
                     VPFFTISGSDFVEMFVGVGASRVRDLFNQAKAKAPCIIFIDEIDAVGRHRGAGLGGGN
                     DEREQTLNQLLVEMDGFTVDQGIIILAATNRPDILDPALLRPGRFDRRVYVNYPDSKG
                     RYEIMLVHTRNKKLASDVDLHEIAKITPMFTGADIENLMNEAAILAAKRNKDEITMEE
                     VRESIYKVAMGPEKKSRIISDEERKLTAYHESGHAIAMRTISSTAKVQRVSIIPVGQA
                     GGYTMPIANEDKYFYTKQQLQEEIMTLLGGRAAEAIALGEVSTGASNDLQRANKIVRN
                     MIVKYGMSESLANIVFQEEDEVFIGKDYSKVKSYSEGIATKIDQETKRIIDECYDKVK
                     AILTDKRNILDTLAHSLLENERVEADEFEKIYNGENPFEVATLPA"
     gene            9478..10644
                     /gene="metK"
                     /locus_tag="PLF14_07605"
     CDS             9478..10644
                     /gene="metK"
                     /locus_tag="PLF14_07605"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOT71188.1"
                     /translation="MVNRKIFTSESVTEGHPDKLCDQISDAILDEILKKDKEARVACE
                     TAVTTGLIHVMGEITTDCYVDIPRICRETALDVGYTRAKFGFDGETCGVITSIDEQSP
                     DISMGVNNSYDLAGEQGAGDQGMMFGFACNETEELMPYPIQTAHTLCKKLAKVRKESD
                     SKYLRPDGKSQVTVEYEGHTPKRIDTIVLSSQHSDEISIEKLREYLTEEVILPAVDKR
                     MIDKNTKIYINPTGRFVIGGPMGDSGLTGRKIIVDTYGGMGRHGGGAFSGKDPSKVDR
                     SAAYAARYAAKNIVASGVAKRCEIQIAYAIGVARPVSVVVDTFGTASVDDERIAKAVM
                     QVFDLRPYSIIQSLNLKSPIYKQTATYGHFGRSDLNLPWEKTDKADILRDMLVK"
     gene            10740..11312
                     /locus_tag="PLF14_07610"
     CDS             10740..11312
                     /locus_tag="PLF14_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOT71189.1"
                     /translation="MDGIIQYIFPNEKYDEINKLKFQAYIKDNHIYFVEYDEVISSVI
                     KYAKYTRHPASFVQIGHEMGVILKDMCVSADNVIYVPMHVKDQAKRGFNQSLILARKI
                     SECTGIPICHKALKKNKKTRHQASLSATLRARNLAGAFVADDRYTAGRSFILVDDIYT
                     TGTTLNQCKKVLLEKMAQSVLFATITKRII"
     gene            11370..12740
                     /locus_tag="PLF14_07615"
     CDS             11370..12740
                     /locus_tag="PLF14_07615"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT71190.1"
                     /translation="MFYSERYRIIQPLLKNSKETKYLVFDEMDKCKKVISENMGKFES
                     GGDIEQLSGIEHTGLPKITDYFEFNECCCIVQEYIEGESLKNYVERRGVLDLYEALDI
                     FIKLLHIVKFLHDIKPVGLIHGSIHHDNVILIEDNVYLTDYGIKEEKKNPYQAPEAIL
                     GIKKSKQQDIYSLGMLLNFMITGGFKKPGYSQKSLTGTDSLIARCTNANFNNRFEDTG
                     ILITECERLARNLTKDSSQPLSKLIAVNGCLDAVFELAQSLGKALSENVLIIDADILQ
                     PSFEPDSYILNDNLQSMMDRADKGEDINKVLKKAKISSKVYIIPSVIRIEGYENITFT
                     SFYNLLIRLAEDFKVIFIKCSDFIYDSLTVNTYIISDQIVYIMSDPLKDMLLFNEISR
                     YLCERHDIDNRRFNAYIYKKRIKNIVTAGIKENLYGEYLGYVNSRSKRYAKDLTGITD
                     KIRRKL"
     gene            12742..13935
                     /locus_tag="PLF14_07620"
     CDS             12742..13935
                     /locus_tag="PLF14_07620"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOT71191.1"
                     /translation="MAVLKSLSILNDLPDNSRYAKGSYPLSKVVTDVTKEILSASPEL
                     VVNAYYDNRQRSFLENRILNILDRQQYFAIGSKRKMIKSIFDFMFGYGQLQEYIEDDE
                     ISDIDGVRYNCFSVTKNGVRMPADIDFCTEADFFVYCKLIALRNGGILNENDSHCRVA
                     DIRNKLRINISIHPRNISGPALSIRKHRRNAYTLEDLNEIGMLDSTCTDILMETLLSD
                     KTIILCGKGGSGKTTLLRALINVLPEMERVLIVESDAEIYPDKLYCIEQRVKRDNEGG
                     NPITLDMLIKDGLTMSLDTYCIGEIVGSEAMAFLNACCSGHRFLTTMHADKPKDCAKR
                     LVALAKGAKGISDEKMLLSLFGEGVDCIVFMQDFAVTEIMNVKGIDTALNSVFYENVY
                     NRRRE"
     gene            13932..14729
                     /locus_tag="PLF14_07625"
     CDS             13932..14729
                     /locus_tag="PLF14_07625"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOT71192.1"
                     /translation="MIYLLINVLFFTVLAFVMNRKNINYDFKLILKKHLGKIIAVSCI
                     VIFIVLGTIINKILNNPVVGYCIALMPAFLPYLVYDLVKSATFKKEQQGITSLLMVLT
                     KWSSVRNDVVYCLQKTDETKLSEPIGGYIKEMCIRLEKGMSVSEALSACQENALSEEL
                     RYVLINIRYAYEKGGSLYRIFKSLENQFFKIDEENFKRKINTLSDKYAVYLSIVMVMA
                     TFYMVVLNKGQSGQYYLKTETGMLLLGVFALLFFAGMLIITKVVKRY"
     gene            14732..15523
                     /locus_tag="PLF14_07630"
     CDS             14732..15523
                     /locus_tag="PLF14_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71193.1"
                     /translation="MSEWVRSIIYLGNFGVGILFASHIACMKLGSRDKKTTFGRIEDA
                     ISGFLCKISEKMGIPEEKTKDFTAITIFFAILCLVVAVTAGIVKGFIILIPCVICIYL
                     MKKAWEKNIGEMFQKNAYRLYRYLVSQITAGVKPKDVIINMYKITEDKSLKKILTRAS
                     AAYSITLDGKTYSDVIKSSVKTEDADRFCMILEEDLLYTDNEEFLEKMEEMMFNRHFS
                     YHQKKTDSIKRGCFAAVCVFMLIVIIMVALPLITELVQGLKYIFS"
     gene            15536..15727
                     /locus_tag="PLF14_07635"
     CDS             15536..15727
                     /locus_tag="PLF14_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71194.1"
                     /translation="MIVNQQSKRGFGISEILGIATVLIIAAAVVIPGLRDLARTILES
                     TKTWVQGKMGTIFNLAPGN"
     gene            15738..16112
                     /locus_tag="PLF14_07640"
     CDS             15738..16112
                     /locus_tag="PLF14_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71195.1"
                     /translation="MAERIIVGIVSILIILCVFVYCTDVLTVISKNMEFYDICRGYMH
                     IAEQNSGLSYEEKTRLKERLIAGGFDNVVITAPEAAVYGSMFNLNVKASYGINMITDI
                     FTRNIQEYVMNFDQNIAARKIR"
     gene            16114..16569
                     /locus_tag="PLF14_07645"
     CDS             16114..16569
                     /locus_tag="PLF14_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71196.1"
                     /translation="MERKKKGSGISEVLISVFVLIYIMLPLFCSVVNKAMFYHQCARI
                     QQAVEMAVISLIRENSTDDFSEGKLSIHTGEEKLKSMIFEGISHSCISTLEVEKDNIC
                     ISLREAGNICSCGTYSQHFIIAADITATLHFLGEREFTVHKHMEFPVVR"
     gene            16635..17264
                     /locus_tag="PLF14_07650"
     CDS             16635..17264
                     /locus_tag="PLF14_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71197.1"
                     /translation="MKRYKYYILAGIFAVLLTVLQIFLSKAVTEKDKGHVYVAQREFL
                     KGEHINAGDVKKVQVYGITSTFTIDESKICITDIPEGTILSDLMLGDLPPDKGLRTMC
                     LPVDKNRCPSAVLTLSDTIDVYIIPDDKSISLSQSIWLDKMLEELKIPYNPDNDIGFM
                     IENLEIADLGNYESSNYTISIKITDSVDELLSFMKGRSTMEIILPKSGN"
     gene            17425..18246
                     /locus_tag="PLF14_07655"
     CDS             17425..18246
                     /locus_tag="PLF14_07655"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015615862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOT71198.1"
                     /translation="MDKYYENLLESVEYIEKRIKDVPVLGIVLGSGLGMLADKIEDKI
                     ELDYKDIPNYPVSTIPGHAGRLIFGYLSGKYVACMSGRFHYYEGYEPNQIHIGVRTFK
                     LLGAKGVIITNAAGGINTSFNEGAIMLIKDHIGFLAPSALRGPNIEEFGERFFDMTRV
                     YDRQYLDTARKVAKKLGIDVKEGVYMFFKGPNFETPAEIRAARILGADAAGMSTVPEV
                     VACSHCGLKVLGISVITNMAAGILDKQLSHAEVMETANRIRKEFVEYVEAIVKEL"
     gene            18252..19496
                     /locus_tag="PLF14_07660"
     CDS             18252..19496
                     /locus_tag="PLF14_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007044319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOT71199.1"
                     /translation="MEYDIKDLSLKNIGREKIEWVKDFMPVLSSIEKKYIDKQPLKGI
                     RVSMALHLEAKTAYLAIVMKNLGAEVRVSGSNPLSTQDQICAALVDMGISVFAYRGVD
                     NETYNRHLVQALSHKPHIIIDDGADLAHALHKKHPELRENVYGGCEETTTGVIRLKAM
                     DRQKILKYPMFAINDADCKHLFDNRFGTGQSSLEGVMRSTNLSLFGKKVVVAGFGWCG
                     KGIAEKAKGFGANVIVTEVDAVKALEAAMEGYEVMPMKQAAKIGDIFITSTGNIDIIT
                     KEHFVLMKNNAILCNAGHFDCEISMKQLNKIAVKKEDRRNGVTGYHLKNGNVINVLGE
                     GRLVNLACSDGHAAEIMDMSFALQLLTALYVLDNKGKLENRLYNVPVEIDRAVAEAKL
                     ESMGICIDVLSAEQKKYLESWE"
     gene            19498..21165
                     /locus_tag="PLF14_07665"
     CDS             19498..21165
                     /locus_tag="PLF14_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71200.1"
                     /translation="MKFSVKDRIIPCPKNIKARGSFTFFRSRTKVNLDVDMSPVIASA
                     YEMLHSLCLPGYLDPEFVITLKKIDQQYEGKELDKCVNKKQAYVIKPVMKDKVYSGVD
                     LLAYTDVGLYYACSTLYQTVIPVSDKEIEIPVLEVSDWPSIEYRGLFLGDYVTCLSHT
                     SSLKLNALDYVYSTKNKEKEKEIYDDCARFGITPFTAVFKWDDPADIFKTLYKKEHKN
                     NLLFWMDEQWDDVEIYHETVKILEAFSQYSKEDDCLEAGIITNFKGDKTFERIFNLKL
                     PENFSISYCDKIKTYNTDVREITDASIAAFTRKDGKFGIYPMIAFDVNTFFPWTAPEF
                     IQFRCAEFDRIRAHKIMGYTPYSCSLLKFNIAAFAEWLWNPKGRNTIDFIKAYAYREK
                     LDFDKYENMLYYLTFSSWSLQRSNFVQCLNEETVDFKNLKKLTRPNQMASDAQRACKY
                     AKLMDNPILVSEAQATYSAIEAYDYTRKLLKLSSRRNPDRNRYIVYKERLNEACKAVY
                     TSLMEWGEHMKQAGAYDTYIHETAKAFLHLTSKFNRFSAEAEKTGEE"
BASE COUNT         6875 a         3594 c         4946 g         5828 t
ORIGIN      
        1 acagattttt ttccttcgga gacgccatgt ttattacgga ttctgataca ataagacggg
       61 aaaagaaaaa tgagtagata tcctgtaaca tatgagcata tacatacctg taaacaatcg
      121 ggagcaaggt tgggaattgt acacactccc cacggctctt ttgaaacgcc ggtttttatg
      181 cccgtaggta ccgcagcgac ggtaaaaggt atgatgccca aggatttaaa ggaaatagga
      241 gcacatatca tactgtccaa cacctatcat ctgtacttac gaccttccga tcaagtggtg
      301 gccgaagcgg gcggattaca taagttcatg aactgggacc gttccattct taccgacagc
      361 ggaggatttc aggtattcag tctcaatact ttgcgaaaga taaccgaaga aggggtaact
      421 ttcagaagcc atatagacgg ttcatcgcat ctttttaccc cggaaaaggt tatgtccata
      481 caaaacaact taggtgccga cattataatg gcgtttgacg agtgtgcacc gtatcccgcc
      541 gaaagaagct atattgacga ttcactctta cgtactgcaa gatggctgga acgctgcatt
      601 aacagtcata acagaagtag cgaacaggcc ttgttcggaa ttattcaggg cgggatgtat
      661 aaggatttgc ggattaaaag cgctaaacag ataacatcct ttgatcttcc gggatatgca
      721 atagggggtt taagtgtagg ggaacctgca gaagttatgt atgaaatgct ggagcatgta
      781 accccgctga tgccgtttga caagcccaga tatctgatgg gtgtcggtac tcccgactac
      841 cttatagaag gctctataag gggaatagat atgtttgact gtgtgcttcc caccagaatt
      901 gccagaaacg gtaccgttct gacaagtaag ggcagagtca ttgtgcggga tgccaagtat
      961 tccagggatt tcacgccttt ggatgacgag tgtgattgtt atacatgtaa gcactttacc
     1021 agagcctatc tgcgtcatct atttaaaagc gacgaaatat tggcattaaa ccttgcaacg
     1081 ttacataatt tgaaattttt acttgactta atggcgaaaa taaggcaatc tataaaggaa
     1141 gacaaacttt tggatttcag agatgaattc tatgaaaagt atggatatta gaaattgaat
     1201 cggaggttat ataaatgttt aatttaattc tggctgaaac ggcggctaca gctgaaacag
     1261 gcggcaggaa ctggacatca ttaatactta tagtagttgt attcgtggta atgtatgcgg
     1321 taattcttat tcctcaaagg aagaaagata agaagctgaa acagatgctt gcggatctta
     1381 aaaacggtga tgtaatatgc acaataggcg gcattatggg aaagattgtt aatgtgcagg
     1441 aagatgagat taccattgag acatccattg agagaaccaa gatgaccatg aagaaatggg
     1501 cggtaagaaa tgtcgagacc gtggaagaag agtcatcgga tgcggagcct acgggcgaga
     1561 ttgaaagcaa gtaagttttt ccgataagaa atacaaagag actgttaaga aacaaaaagt
     1621 taacagtctt tttttgttct caaaatacag ttatattaaa aaatatcggt gtcgtgtgtt
     1681 gttaaatata atataatgca cacatgaata gtaaagagag gttacacctt ttggatgaaa
     1741 tacgcggttt tcttatcata ggtgttgtga tatcacatac tctgttcgat atgcactatt
     1801 ttttcagtat ggatatgtct tggataaagt cccctctttt taccgccatt tccgatatcg
     1861 gagccattat cttcattctc atatccggta tggtttccga gttttccagg gataacataa
     1921 acagaggatt gaaactgatg tgtgttgccg caacggtgac tcttgtaaca catattgttg
     1981 ttccggacta tgtcgtatat gcgggtatac tgcaccttct ttcgtttgct attgtgcttc
     2041 atgaattgat taaaccgctt cttgtgaaga taaacaggat tgcgggtctg gttatcaata
     2101 tattgattgc tctgctgact gcaaatttgt acggcagaga aataagcatt ttcggggtta
     2161 ccttatttaa aataccggat aatatcatga acgggggatt atcttacctt ctgggactta
     2221 agatagataa tataaccgtt tcttccgact actatcctat ccttccctgg attacttttt
     2281 tctttatggg gttttatctg acagagtaca ttgaaaggca acctcttaag tcttttttgt
     2341 gcaggaaaag atgcagtttt ctggccaagg cggggaggaa ttctttactg gtttatttaa
     2401 ttcatcaacc ggttgttatg ggaatactgt atcttatcag tttgatataa agagaaaagt
     2461 ggtgaaaaga ttgatatatt cttcctgttt ggagtttttg atgtatacat cggtaaccgt
     2521 aactttatac gcattgatat aagagccttc ggaataatag ccggtaatat cgtggatgat
     2581 atagggtttg tcctgtatta taccgagata aagcataacg tgaccgtcca tgaaaatcaa
     2641 tgatcccgct ttttgagttt tcagcagttg ctctttttcc tgtttactca taccttttac
     2701 gtttgttttg ctgggtgtca ttgtctcctg ctgatcggta tttcttttaa gttttattcc
     2761 catggaacgg aatatatcca taacaagcgc cgagcaatct ctgtatccgt tcatgccgcc
     2821 ccaaccgtac ggttcattaa gaaacttttt tgcctgaatg agtatatttt tttgagtata
     2881 gggaagatag ccgacatgtg cagactgctt ctctacttta tcaaaggaaa aatacagggt
     2941 accgtccgca ccttgcttgg gacataacac ggtgtaataa ttgtcatgct ctttgaccaa
     3001 gggaaatacg cagcccatat ccgattttgt gtgtgagtag cgaaggtatg caataggtcg
     3061 ggttatgact atgaagtttc tgtttttctc atacaatttc acaatgtact ccgatgtaac
     3121 cgcaacgtcg gctttattaa tccagccttc atagttatac atttttataa acaaccattg
     3181 tttatccttg ctttgatgta aaattgcaac cggttcaaag ggataaaggg tggattctct
     3241 gaatctgtcg tggacggaag aaggtgagga gaatacctcg tcgtaagttg ggaaagcctt
     3301 aagtgcggac ctttttataa caaatccgta tcggatatta tcagtatccg tatccttaac
     3361 ggagtcataa tcaatattat caataagcct tttgtaataa tcgccgaata tctccgcata
     3421 tgaaccgtcc gtgttcttta tgtatctttt tgcctccggt attacgctta tcttctttat
     3481 tttactaact aaatcctctt taaacagggg ggtgtttatg tatatgctgt cattctgttt
     3541 attccattta tctatggacg tataatccat tatcgtatct tccgaggcgg cggatatagc
     3601 ctcacgtaaa atgtctgctt tacagtaagt tgttgtagag ggagtaagtg tgataagcag
     3661 ggttacaatc agtactgtta ttattgcgga taaaagcttt ttcatgttat cattatatca
     3721 tcgcaaataa atgttttttg aaaacgtgtt attgtgaagt ataataactt taaacggttg
     3781 accgattcgg aggagaatat gaaagtaata ctcacacaag acgtaaagaa tttgggaaaa
     3841 aagaatcaac tggttaatgt aagtgacgga tatgcaagga attttctgtt taagaaaaat
     3901 cttgctgtgg aagccaatgc caagaacctc aatatcatga aagacagaaa gcagtcggag
     3961 agttcaaaaa aagacagaga gcttgttgag gctaatctgt tgcagtccag actggaaggt
     4021 aaacaggtaa aaatggaggt cagagcgggc gagagcggaa aactgttcgg ctccataacg
     4081 gcaaaagaca ttgcggatgc gatagctgaa cagtataaag cggatattga cagaaggaaa
     4141 atacagcttg atgaacccat caagaataca ggggatgcgg atgtagttat taagatacat
     4201 cccgaggtaa gtataaatat ccgcctggtt atcacggaga aagtataata tgagtaaaga
     4261 tgtcaaaaga gtgcctcctc acagtcagga ggccgaacaa tatgttttag ggtgtctgtt
     4321 gttggacagt tccgcatttg aaccggttca ggttaagatt gattcttcca tgttctatat
     4381 ccccgcacat agggatatat acaatgcgtt gcaggcagtt ttcttaagcg gtattcccgt
     4441 ggatatagtg tcggtagcgg aacagatgaa aaaagacgat acatttaccg atgccggcgg
     4501 catggaatat ctcaacaaac ttgcatcaca ggtagtgtcc gcttcctctg ctgagtatta
     4561 tgcatccatt atctttgaca aatacatatt gcgttcgctc atatctctca gcggtcaggt
     4621 gtttgaggat gcatacagcg attcaaagga agcaaaggaa cttctggaat ataatcagca
     4681 ggaacttttc aatctttccg aacaagccga taccaaaggc gtaaaaaaga taagcgatct
     4741 ggttgtgcgc aggctggact atttccgtca ggtaagtaag acaaaggaaa ttcccaactg
     4801 tatcgaaacg gggtttgccg acctggataa attgatattg ggcttccgtc ccggtgactt
     4861 tatactcatc gccgcaagac cttccatggg aaagtccaca ttggctatga acatggctca
     4921 gaacatggca ataagaaaag gaaagaatgt gctgttctta agtctggaga tgtccaatga
     4981 gcagttgacc gacaggatga ttgcttcgga agccagagtt ccgaatttaa cacttcaacg
     5041 cggtgaagcc gatcaaaatg aatgggccaa ggttgcggca accgcaggca taatacatga
     5101 atccaggata tttcttgatg atacctcatc cattaccgta gcggatatac ggtccaaatg
     5161 caggcgattc aaagctgccg agaatctgga tattattttc atcgactatc tgcaacttat
     5221 cggaagtgcg gtaaagaagg aaaacagaca gcaggaagtt accgaaatat ccaggcaact
     5281 taaaatattg gcaaaagatt tgaaaatacc ggttgttgcg atgtcccagc tttccagagc
     5341 ttccgagcag agagccgatc gtgttccgca gttgagcgat cttcgcgatt ccggagccat
     5401 agagcaggac gccgatatgg tcatgtttat tcacagaccg caggaattgg aaaagaatgc
     5461 aacaacgggt attgttaatc tcatcgttgc aaaaaacaga agcggttcca ccgggactgt
     5521 tcaactggtc tggcaaggac cgatagtcag atttgacagt tataccaaaa gagatgatta
     5581 agctcgattt ggacgataat ttaattaaag ataaaccctg gtatatcgcc ttttccgggg
     5641 gacccgattc cgcatgtctt ttatatcttc tgatgaagaa aagagaagag attgagaaaa
     5701 aatacaaagt ttcggttaaa ctttcagcca ttcatgtaaa tcataattta cggcaatcgg
     5761 agagcaaaag agatgaagat ttttgcaggc ggttatgtga gaaatatgac atagagtttc
     5821 tgccttatga tgtggatgtc aataactatg cgaaagaaga gaaactgaca gtggagcagg
     5881 ctgccagggt gttgcgatat gagatttttg acgattattc ctacgggtgt gtatttttag
     5941 gccataacaa agatgacaat gccgaaacgg ttttcttgaa tatattgaga ggaagcggtg
     6001 tgcagggact gtgcggtatg cagaaggtat ccgagcatta cttcagaccg ctcttggatt
     6061 atacaaaaaa ggaaatactg gagttcaacc gtgaaaacgg tattgaatat gtaatagaca
     6121 gtacaaaccg ggccagttac tatatgcgga attatataag aaatgagatt attcccgcac
     6181 tcaacgagaa gacaaataag gatattgtag gcaatctgat tgcattggcc gatattgcaa
     6241 cgcagacgca atcatatatc aataaacaga ttgatgccga atgggataag agagtgacgg
     6301 cgggaaaaga ccatgttctg gtggatgtta aagattttgt cgatatggat caggtcatat
     6361 cctcggggct tttaagaaaa gccataaggt atgtcaaaaa aggattgaag gacgtcgaga
     6421 agaagcatat agagattgtt cacaccctta ttctccacag tcaaagcgga gcggttgtcg
     6481 atttgaaaga cggtataagg gtacttcttt tgcaaaataa cagggtgaag atttatagac
     6541 agtccgatga ggattatgat ttttgtgttg ccgtaaacat tgaaggcgat acgtttattg
     6601 aaagcagaaa cataaatgtc cgggcctccg cctgtatatt tgaaggcggc gatgagtata
     6661 ccgaacaacg ggtttttttg gattttgagg cggtaaagca agggttggtt ataaggaaca
     6721 ggaaatcccg cgatttcata tatccggcaa aaggtaacgg aaagaagaca ttaaagaagt
     6781 attttataga taaaaaggtg gacagcgacc ttcgggacag gctttttgtc cttgcgattg
     6841 aaaatgagat agtgtatatt gaaaacaagg aaataggaaa gagatttcgc cccgtaaaag
     6901 ggagtaaggc attgagattg gagtttgtaa agtatgaacg aactgtataa cgacgtcagt
     6961 gagataatga taacaaaaga gcggctggat gaaatatgtg cggaattggg taagagaata
     7021 tcctcagact ataaaggaaa gcagctcatg cttgtaggag tgatgaaagg cgggcttatc
     7081 ttcctgtccg atctgttaag aaagataacc attccggtgg agttcggtac cataaccgct
     7141 tccagttacg gtaagggtac atcttcttcc ggtcaggtga ccattcttca ggacctggat
     7201 atggatatag cgggaaagga tgttatcatt gtcgaagacc ttatagacag cggacatact
     7261 ttgagatatt tacaggaatt cttaggttta agaaatccca acagcgttaa gatatgctgt
     7321 atgctggaca aaccgtccag acgggaaaaa caggtaaagg tggactatat aggtcaggaa
     7381 atacccgata aattcgtggt cggctacgga atggatttcg aggaaaaata cagaaatctt
     7441 gccgatgtat gcgttttaaa gaaagagttt tacgagtaga ataattgaag tttacgtgaa
     7501 ctttgttgca atatatgtgc ccgtgtgcta atattctgta tcaggtataa ggagaggact
     7561 tttgaaaaaa tacagaaata taagttttta tgtaatattg tttataatag cggtgatact
     7621 tatctcgctt tttaccggtc gtgattcggt aaaagaattg ggttattcgg agtttctgaa
     7681 tcaactggaa aagcagaatg tatcggaagt tattattcgc acaaccactc tcacggtaac
     7741 tcttaaagag cctattaagg acggcaacaa ggtttttcat gtaaatgtgg agaatcttga
     7801 caagattgaa gatatattaa acgaggccag gctcagcgac cccgatcttt cctatacatg
     7861 gaaaccggaa acgacatcct ggtggttatc cttacttcct tcgttcataa tactggcaat
     7921 tgtaataata gttttctttg tatttatgaa taaacaggcg ggagcaaata attccgcaat
     7981 gtctttcggg aaaagcaggg caagagaata cagcgaaaag gacagaaaag tcacatttct
     8041 ggatgttgcg ggagccgatg aggaaaaagc cgagctggaa gaggttgtgg atttcttaaa
     8101 gcagccggaa aaatacaaga aattgggggc aagaatacct aagggtatac tcatagtggg
     8161 acctccgggc acaggtaaga ctttgttggc acgtgccgtt gccggtgaag cgggcgttcc
     8221 gttttttacc ataagcggat cggacttcgt ggaaatgttc gtaggagtag gagcttcaag
     8281 agtaagagat ctgttcaatc aggctaaagc caaagctccc tgtatcatat tcatcgatga
     8341 aatagatgcc gtaggtcgtc acagaggtgc aggcttagga ggaggcaacg atgaaagaga
     8401 acagactctc aaccagttac ttgtggaaat ggacggtttc accgtagacc aaggcattat
     8461 aatactggcc gcgaccaaca gaccggacat tttagatccc gctcttttac gtccggggcg
     8521 gtttgacaga agagtatatg tcaactatcc cgattccaaa ggcagatatg agattatgtt
     8581 ggttcataca cgcaataaaa aacttgcttc cgatgtggat ttacatgaaa tagccaaaat
     8641 aacccctatg tttaccggag cggatataga aaatctcatg aatgaggcag ctatactggc
     8701 cgcaaagaga aataaagacg aaataaccat ggaggaagtg agagaatcca tttacaaagt
     8761 ggcaatgggg ccggaaaaga aaagcagaat tatatccgat gaggaaagaa aacttaccgc
     8821 ttatcatgaa tcgggtcatg ctattgccat gagaaccatt tcttccaccg ccaaagttca
     8881 gagagtatct attatacccg tagggcaggc aggcgggtat acaatgccca tagccaatga
     8941 agataagtat ttctatacca agcagcagtt gcaggaagaa ataatgacat tactgggtgg
     9001 ccgtgcggcg gaggctatag ctttaggaga agtaagcacc ggagcttcca atgacttgca
     9061 aagggcaaat aagattgtaa gaaatatgat tgtcaagtac ggtatgagcg aaagtcttgc
     9121 gaatatagtt tttcaggagg aagatgaagt tttcatagga aaagattact ccaaggttaa
     9181 aagttactcg gaaggtatag caaccaaaat agatcaggaa acaaaacgta tcatagatga
     9241 gtgttatgat aaagtaaaag cgattttaac cgataagaga aatattttgg atacattggc
     9301 acattctctt ctggaaaatg aaagagtgga agccgatgag tttgaaaaga tttataacgg
     9361 agagaatcct tttgaagtgg caacattgcc ggcttaatgt gaaaccgaaa aattcggcaa
     9421 ataataagca ggattagttt aatattgtaa atttatctat tatatgggag gcaatatatg
     9481 gttaacagaa aaatatttac atcggaatcg gtgaccgaag gacatccgga caaactgtgc
     9541 gatcagatat ccgatgcaat tcttgatgag attcttaaaa aagacaaaga ggcaagagta
     9601 gcatgtgaaa ctgcggtaac aacgggtttg attcatgtaa tgggtgagat aaccactgat
     9661 tgctatgtgg atataccgag aatttgcaga gaaactgccc tggatgtagg atatacaagg
     9721 gctaaattcg gttttgacgg agagacctgc ggagttatta cctccattga tgagcagtca
     9781 ccggatattt ccatgggtgt aaacaactct tatgatcttg cgggagagca gggagcggga
     9841 gatcagggaa tgatgttcgg ttttgcatgt aatgaaacgg aggaactgat gccttatccc
     9901 atacagacgg ctcatacctt gtgtaaaaag ctggcaaagg tcagaaaaga gagcgactcc
     9961 aaatacttaa gaccggacgg taaaagtcag gttaccgtag agtatgaagg acatactccc
    10021 aaaagaatag ataccattgt attatcctct caacacagcg atgagatatc cattgagaag
    10081 ttaagagaat accttaccga ggaagttata ctgcctgcgg tggataaaag aatgatagac
    10141 aaaaatacaa agatttatat caatccgacc ggaaggtttg ttataggcgg acctatgggc
    10201 gactcgggat tgaccggaag aaaaatcatt gtagatacat atggcggtat gggacgtcac
    10261 ggcgggggag ctttttccgg taaagatcct tcaaaagtag accgttccgc agcatatgcg
    10321 gcaagatatg cggctaaaaa tatagttgca agcggtgtgg ctaaaagatg cgaaatacag
    10381 atagcatatg caataggagt ggcacgccct gtatccgttg tggtggatac attcggtacc
    10441 gcttcggtgg atgatgaacg cattgccaaa gcggtaatgc aggtatttga tttgcgcccg
    10501 tactccatta ttcaatcgct caatcttaag agccctattt acaaacagac ggctacttac
    10561 ggacacttcg gaagatccga tctcaatctg ccgtgggaaa agaccgataa ggcggatatt
    10621 ctgagggata tgcttgttaa ataggttgtt tataagatta ataacaggat ggatttaaaa
    10681 acagagacta cttgaagtag tctctttaat agcacaattt gtagacatat aatgtaagaa
    10741 tggacggtat tattcaatac atatttccca atgagaagta tgatgagatt aataaactta
    10801 aatttcaggc atatataaag gataatcata tatattttgt agaatatgat gaagtgattt
    10861 cttccgttat aaaatacgcc aaatataccc gtcatcctgc gtcttttgtg cagatagggc
    10921 atgaaatggg agtaattctg aaagatatgt gtgtaagtgc ggacaatgta atatatgttc
    10981 ccatgcatgt taaagatcag gcaaaacgag gcttcaatca atccttgata ctggctcgta
    11041 agatttccga atgtaccggt atacctatat gtcataaggc attgaaaaag aacaaaaaaa
    11101 cccgacatca ggcatcgtta agtgcgacat taagggcaag aaatttagca ggagctttcg
    11161 ttgcggatga cagatatacg gccggaagaa gcttcattct tgtggatgat atctacacaa
    11221 cgggcactac tttgaatcaa tgtaaaaagg ttcttttgga aaaaatggct caatcggtgc
    11281 tttttgctac cataacaaaa agaattatat aattgtatat tgacaaatat tgtaaatata
    11341 ttatcatgta cctgtaatcg ggaggaatta tgttttacag tgaaaggtac aggataatac
    11401 agcctttgct taagaactca aaagagacaa aatatctcgt gttcgatgag atggataaat
    11461 gcaagaaagt tataagcgag aatatgggca agtttgagtc aggcggtgat attgaacagt
    11521 tatccgggat agaacacaca ggattaccca aaataaccga ttatttcgag tttaacgagt
    11581 gttgttgcat agtgcaggaa tatatcgaag gcgagtcatt aaagaattat gtggaaagaa
    11641 gaggtgttct ggatttatat gaagctttgg atatatttat taaactctta catattgtta
    11701 agttccttca tgatataaag cccgtcggtt taatacacgg tagtatacac catgataatg
    11761 ttatacttat cgaagataat gtctatttaa ccgattacgg tatcaaagaa gagaagaaga
    11821 atccctatca ggcaccggag gctatattgg gtattaaaaa aagtaagcag caggacatat
    11881 attcattggg aatgttgttg aactttatga ttaccggcgg gttcaaaaag ccgggatatt
    11941 cgcaaaaaag tcttaccggt accgattcgc tgattgcaag gtgtacaaac gccaacttta
    12001 ataaccgatt cgaagatacc ggtattctca ttaccgagtg tgaaagactg gcaagaaatt
    12061 tgacaaaaga cagctcacaa cccctgtcaa aacttattgc cgtaaacgga tgtctcgatg
    12121 ctgtatttga actggcacaa tctctcggta aagctttgtc ggaaaatgtg ctaatcatcg
    12181 atgccgatat actgcagcct tcctttgaac cggacagcta tatattaaat gataatctgc
    12241 agagcatgat ggacagagcg gataagggcg aggatataaa taaggtgtta aagaaggcaa
    12301 aaatctcttc caaggtatac ataataccaa gtgttatacg tattgaagga tatgaaaaca
    12361 taacttttac ctctttttac aatcttctta tccgactggc cgaagatttc aaggttatat
    12421 ttataaagtg ttccgacttc atatatgact cgctgaccgt aaatacatat atcatcagcg
    12481 accagatagt atacatcatg tccgaccctt tgaaggacat gttacttttt aacgaaataa
    12541 gcagatatct ttgcgaaaga catgatattg ataacaggcg attcaatgca tatatctaca
    12601 aaaaaagaat taaaaatata gtaacggcag ggataaagga aaatttgtat ggtgaatatc
    12661 tcggatatgt aaacagtcgg agcaaacgat atgccaaaga tttaaccggt attaccgata
    12721 agataaggag gaaactgtga tatggcggta cttaaaagct tatccatatt aaacgactta
    12781 cccgataaca gcagatatgc caaaggcagt tatccgctgt caaaagtagt caccgatgtg
    12841 accaaagaga ttttaagcgc ttcaccggaa ctggttgtca acgcatatta cgataacaga
    12901 caaagaagtt ttttagaaaa cagaatactg aatatattgg acagacagca gtattttgca
    12961 ataggctcca aacgaaagat gattaaaagt atatttgact tcatgttcgg atacgggcaa
    13021 ctgcaggaat atatagaaga tgacgagata agtgatattg acggggtacg ttataactgt
    13081 ttttccgtta caaagaacgg agtaagaatg cccgcggata tcgatttttg taccgaagcg
    13141 gacttttttg tttactgcaa attgatagct ttaagaaacg gaggcatact caacgagaat
    13201 gacagtcatt gcagggtggc ggatatacgc aataaacttc gtatcaacat ttccattcat
    13261 ccgagaaaca tatcgggacc ggcactatcc atacgaaaac acagaaggaa cgcatataca
    13321 ttggaagatt tgaatgagat cggaatgctg gatagcacat gcaccgatat tctaatggaa
    13381 actttacttt ccgataaaac cattattctg tgcggaaaag gcggatcggg aaaaaccact
    13441 ctcttaagag cacttataaa tgtattgccg gaaatggaaa gagtacttat tgtggagtcg
    13501 gatgcggaaa tatatccgga caaattgtat tgtatagagc aaagggtaaa gcgggataat
    13561 gaaggcggta atcccataac tttagacatg cttatcaaag acggtcttac catgtcgtta
    13621 gatacttatt gcatagggga gattgtaggc agtgaagcca tggcattttt gaatgcctgc
    13681 tgttcgggac acagattctt aaccacgatg catgccgata aaccgaaaga ttgtgcaaaa
    13741 agactggttg ctcttgccaa aggtgcaaaa ggaataagcg atgaaaagat gcttttaagc
    13801 ctgttcggag aaggtgtgga ctgcatagtc tttatgcagg attttgcggt aacggaaata
    13861 atgaatgtaa aaggaattga taccgcactt aacagtgtgt tttacgagaa tgtatacaac
    13921 aggaggcggg aatgatttac ctacttatca atgtgctatt tttcacggtt cttgcctttg
    13981 ttatgaacag gaagaatatt aattatgatt ttaaacttat actgaaaaaa cacctcggta
    14041 agataatagc ggtttcatgt attgtcatat tcatagtttt aggtacaatt attaataaaa
    14101 ttctgaataa tcccgtggtg ggatactgta ttgcattaat gcctgctttc ctgccttacc
    14161 ttgtatatga tcttgtaaag agtgccacat ttaaaaaaga acagcaaggt ataacatcgt
    14221 tgttgatggt actgaccaaa tggagttcgg tacgaaacga tgtggtgtat tgtctgcaaa
    14281 agaccgatga aacaaagctg tccgaaccta tcggggggta tataaaggaa atgtgcatca
    14341 ggctggaaaa gggaatgagt gtttccgaag cgctgtctgc atgccaagag aatgctttaa
    14401 gtgaagaact ccgttatgtc cttataaaca tccggtatgc atatgagaag ggcggaagtc
    14461 tctacaggat tttcaaaagc ctggaaaatc agttctttaa gattgatgaa gagaatttta
    14521 aaagaaaaat taatacctta tcggataaat atgccgtata cctttcaatt gttatggtta
    14581 tggctacctt ctatatggta gtactcaaca aaggacagtc gggacaatat tatcttaaaa
    14641 ccgaaacggg catgttgctt ttgggtgtat ttgcgcttct gttctttgca ggaatgctta
    14701 tcatcaccaa agtggtaaag aggtattaag tatgagtgaa tgggtaagaa gcatcatata
    14761 tttaggtaat ttcggggtgg gcatactgtt tgcttcacac atagcatgta tgaagttggg
    14821 cagtcgtgat aaaaagacaa cattcggaag aattgaagat gcgataagcg gctttttgtg
    14881 taagatttcc gagaaaatgg gaataccgga ggaaaaaacc aaagacttta ccgccattac
    14941 aatatttttc gcaattctgt gtttggtagt agccgtaaca gcgggaatag ttaaaggatt
    15001 catcatatta attccctgtg tcatttgcat ttaccttatg aagaaagcct gggaaaagaa
    15061 tataggcgaa atgtttcaga aaaacgcata caggctttat cgatatcttg tgtctcagat
    15121 aacggcggga gtaaagccga aggatgtgat tataaacatg tataagataa cggaagataa
    15181 aagtcttaaa aagattctga ccagggcttc ggccgcatat tccattactt tggacggtaa
    15241 aacctattcg gatgtaatca aatcttccgt aaagaccgaa gatgccgaca ggttctgcat
    15301 gattctggaa gaggatcttc tgtataccga taatgaggag tttttagaaa aaatggagga
    15361 aatgatgttc aacagacatt tttcctatca tcagaaaaag accgacagca ttaaaagagg
    15421 ctgctttgcg gcagtgtgcg tattcatgct tattgtaata atcatggttg ctctgccgct
    15481 tataaccgag cttgtgcagg ggctcaaata tatattttca taggaggaga agaaaatgat
    15541 agtaaatcaa caaagtaaaa gaggattcgg tatttccgaa atattgggaa tagcgactgt
    15601 attgattatt gcggctgcgg ttgtaatacc gggactcaga gatctggcaa gaactatttt
    15661 agaatccacc aaaacatggg tgcagggcaa gatgggaacc atcttcaatt tagctcccgg
    15721 aaactgacgt aaagcatatg gctgaaagga taatagtcgg tatagtctcc atactcataa
    15781 tactgtgcgt gtttgtatac tgcaccgatg ttcttaccgt tatatcaaaa aacatggagt
    15841 tttacgatat atgcagagga tatatgcaca tagccgagca gaattccgga ttgagttatg
    15901 aggaaaagac ccgtttgaag gaacgtctaa ttgcaggggg ctttgataat gttgttatca
    15961 cagcccctga agcagccgta tacggaagca tgtttaacct gaatgttaaa gcatcatacg
    16021 gtataaatat gataacggac atatttacca ggaatataca ggagtatgtg atgaattttg
    16081 accagaatat agcggcaaga aagatcaggt gacatggaac gaaagaagaa aggttcggga
    16141 atatccgaag tacttataag tgtttttgtt cttatctaca taatgcttcc gcttttctgt
    16201 tcggtagtca ataaggcgat gttttatcac cagtgtgcaa gaatacaaca ggctgtggag
    16261 atggcggtaa tatcattgat aagagaaaac agtaccgatg atttctccga aggtaaactt
    16321 tcaatacata ccggtgaaga aaagcttaag agcatgattt ttgaggggat atcacattcc
    16381 tgcatcagta cgctggaggt tgaaaaggat aatatatgca tatcgttaag agaggcgggc
    16441 aatatttgca gctgcggtac ttacagccaa cacttcatta tagcggcgga tattacggct
    16501 acattgcatt ttctcggaga aagagagttt accgtgcaca agcatatgga atttccggtt
    16561 gtaaggtgaa taagtaaata ttaataatta aacatcaata agctataaac agagaatagg
    16621 gtgaaaaaag agttatgaaa agatataaat actacatttt agcgggcata tttgcggtat
    16681 tgcttaccgt attacagatt ttcctttcca aagcggttac ggaaaaagat aaagggcatg
    16741 tatatgtggc tcaaagagag tttttaaaag gggagcatat aaatgcaggc gatgtgaaaa
    16801 aggtacaggt atacggtatt acctcaactt ttacaattga tgaaagcaag atatgcataa
    16861 ccgatattcc cgaaggaact attttaagtg acctgatgct gggtgacttg ccgcccgata
    16921 aaggtttaag aacaatgtgt ctgcccgtag ataaaaaccg atgtccgtcg gcggtactga
    16981 ctttgtcgga caccatagat gtgtatataa tcccggatga taaatccatc agtttatccc
    17041 aatcgatatg gttggataag atgcttgaag aactgaaaat accatataat ccggacaatg
    17101 acatagggtt tatgatagag aatctggaaa tagcggattt aggtaattac gaaagctcca
    17161 attacaccat cagcataaaa attaccgatt cggtagatga acttctttcc tttatgaaag
    17221 gtcgcagcac catggaaatt atactgccga aaagcggaaa ctgacctgac aacctgtttg
    17281 atataagtat ttttaacagt attaaatgaa aaagagattg ataaagtaag taattataga
    17341 ataagaatat atgtagtttt aagttgtata gaaagacata atcctttatg atataattta
    17401 tcaaggatat tttatggagg ggatatggat aaatactacg aaaatctgtt ggagtctgtt
    17461 gaatatattg aaaaaagaat aaaagatgta cccgttttgg gtattgtgct gggttccggt
    17521 ctggggatgc ttgcagataa gatagaggat aagattgaac tggattacaa ggatattccc
    17581 aattatcccg tttccacaat acccggtcat gccggaagac tgattttcgg ttatttgagc
    17641 ggtaaatatg tagcctgtat gtcgggaaga tttcactatt atgaaggata cgaaccaaat
    17701 cagattcata taggtgtcag aacattcaag ctgctcggcg caaagggtgt cataatcacc
    17761 aatgcagccg ggggaataaa cacttcattc aatgaaggag ccataatgct cataaaagac
    17821 catataggct ttctggcgcc gtccgccctg agaggtccga acatagagga attcggagaa
    17881 cggttctttg atatgacaag ggtttatgac agacaatatc ttgatacggc acgaaaggta
    17941 gcaaagaagt taggtattga tgtaaaagaa ggtgtatata tgttctttaa aggacctaat
    18001 tttgagacac ctgcggaaat acgtgctgca agaatactgg gtgcggatgc ggcgggaatg
    18061 tctacggttc cggaagtggt agcctgttct cactgcggcc tgaaagtatt gggtatctcg
    18121 gttataacca acatggcagc gggaattttg gataagcaac tcagtcatgc agaagtcatg
    18181 gaaacggcaa accgtatacg caaagaattt gtggaatatg ttgaagcgat agtaaaggag
    18241 ctatagaaaa tatggagtat gatattaagg atctttcgtt aaaaaacata ggcagagaga
    18301 agatagaatg ggttaaggat tttatgcctg tcctttcttc cattgagaaa aagtatattg
    18361 ataagcagcc tctgaaaggc ataagggttt ccatggcatt gcatctggaa gccaagacgg
    18421 cttatctggc aattgttatg aagaacttag gagcagaggt aagggtgtcg ggaagtaatc
    18481 ctctttccac gcaggatcaa atatgtgcgg cacttgtgga tatgggaata tcggtatttg
    18541 cttacagagg agtggacaac gaaacataca acagacatct tgtacaggct ctttcccata
    18601 aacctcatat aataattgat gacggagccg atctggcaca tgctctgcat aaaaagcatc
    18661 ccgagttgag ggaaaatgtt tacggcggct gcgaagaaac cacgacgggt gttattcgcc
    18721 ttaaagcaat ggacagacag aagatattaa aatatcccat gtttgccatt aatgacgccg
    18781 actgtaagca tctgtttgac aacagattcg gtaccggtca gtcatcattg gaaggcgtaa
    18841 tgcgttcgac caatctttcg ctgttcggta agaaagtggt tgtagcaggt ttcggctggt
    18901 gcggtaaagg catagcggaa aaggctaaag gtttcggagc aaacgtaata gttaccgaag
    18961 tggatgccgt aaaagctctg gaagccgcaa tggaaggata tgaagtaatg cctatgaaac
    19021 aggcggcaaa gataggtgat atttttatta cctctacagg caatattgat attattacga
    19081 aagagcattt tgttcttatg aaaaataatg cgatattgtg taatgccgga cattttgatt
    19141 gcgagatttc catgaagcag ttaaataaga ttgctgttaa gaaagaagac agaagaaacg
    19201 gagttaccgg atatcatctt aaaaacggta atgtaataaa tgtactgggt gagggcaggt
    19261 tggtgaatct ggcctgttcc gacggacatg ccgcggaaat tatggatatg agttttgcct
    19321 tacagcttct taccgcatta tatgttttgg ataacaaagg gaagctggag aacagattat
    19381 acaatgttcc ggtagaaatt gacagagctg ttgccgaagc aaagttggaa tcgatgggta
    19441 tttgcattga tgtactgtcg gctgaacaga aaaaatattt ggagagctgg gaatagaatg
    19501 aaattttccg taaaagaccg aattataccg tgtcccaaga atataaaggc aagaggaagt
    19561 tttacttttt tccgaagcag gactaaagtc aatcttgatg tggatatgtc accggtaata
    19621 gcaagtgcat atgagatgtt gcattcctta tgcttaccgg gatacctgga tccggagttc
    19681 gtaataacat taaagaagat tgaccagcaa tatgagggta aggaactgga taaatgcgtt
    19741 aataaaaagc aggcgtatgt tattaagccg gttatgaagg acaaagtcta ttccggtgtg
    19801 gatcttttgg catacaccga tgtcgggttg tattatgcat gttccaccct ataccagacc
    19861 gtcatacccg tctcggacaa agagattgaa ataccggtcc tggaggtctc cgactggcct
    19921 tcgatagagt acagaggatt gtttttagga gactatgtta catgtctttc acatacatct
    19981 tctctgaaac tcaatgcttt ggattacgta tacagcacca aaaataagga aaaagagaaa
    20041 gaaatatatg acgactgtgc aagattcggt attactcctt tcactgccgt attcaagtgg
    20101 gatgatcctg cagacatttt taaaacactg tataaaaaag agcataagaa taacctgctg
    20161 ttctggatgg atgagcagtg ggatgatgtg gaaatttatc acgagaccgt gaagatactg
    20221 gaagccttca gtcaatacag caaggaagat gattgtctgg aagcgggaat tataaccaac
    20281 tttaaaggag ataagacatt tgaaaggata ttcaacctga aactgcctga gaacttctcc
    20341 atctcttatt gcgataaaat aaagacatat aataccgatg taagagaaat aaccgatgca
    20401 tctattgcgg catttaccag aaaagacggt aaattcggca tatatccaat gatagcattt
    20461 gatgtaaaca cattctttcc ttggacagcg ccggaattca ttcagttccg ttgtgcggaa
    20521 tttgacagga taagagcaca taagattatg ggctatactc cgtattcctg ctcattgctg
    20581 aagttcaata ttgcggcatt tgccgagtgg ttatggaatc ccaaaggacg aaataccata
    20641 gatttcataa aagcatatgc gtacagagaa aagctggatt tcgataaata tgaaaatatg
    20701 ctgtattacc ttacgttttc ttcctggtca ttacaaagaa gtaactttgt gcagtgctta
    20761 aacgaagaaa ccgtggactt taagaatctt aagaaactga cgcgtcccaa ccagatggca
    20821 agtgatgccc aaagggcttg caaatatgca aaacttatgg ataatccgat acttgtaagt
    20881 gaggctcaag ccacatattc ggctatagaa gcatatgatt acacaaggaa acttctgaag
    20941 ttatcatcac gtaggaaccc tgacaggaac agatatattg tatacaaaga gcggctgaat
    21001 gaagcatgta aagcggtcta tacctctctt atggagtggg gagagcatat gaaacaagcc
    21061 ggagcatatg atacatatat acacgaaaca gcaaaagctt tcctgcatct tacgtctaag
    21121 tttaacagat tttctgccga ggcggaaaaa acaggcgaag agtagctata ttgacataca
    21181 ttgaataaat gatataattt cctcaatacg gcgttactaa aaggatgagt aacatatggt
    21241 ctg
//
LOCUS       DAOSOM010000033        20417 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_2614_length_20417_cov_5.622729, whole genome shotgun sequence.
ACCESSION   DAOSOM010000033 DAOSOM010000000
VERSION     DAOSOM010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 20417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20417
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2614_length_20417_cov_5.622729"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="PLF14_07670"
     CDS             <1..1107
                     /locus_tag="PLF14_07670"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT71201.1"
                     /translation="KKELGEDIAYYTSFDDFINHDMDAVFLANYATEHAPYAIRAFEK
                     GLHVLSEVLPCQTMKEAVELIEAIEKSGKIYAYAENCCFMAAPYEMRKLYRSGQLGEL
                     EYGEGEYLHNCESIWPRITRGDKNHWRNNMYAHFYCTHSFGPFIHITGLRPISVTGFE
                     LPFNARMERMGAKSGSAGLAIITMENGAIIKNINGVGCSKNSLWFSIYGTLGRAESAR
                     EDSQYGMYSRIFTNLDRYEGECFDRPNTYCPGADQPSPAQKWGHGGGDYYIMHNFVDR
                     ISGNTDAETIDIYEALDMFLPGMFAYRSVLAGGIPVNIPNLRIPSERDRWRNDTWCTD
                     PAVAKDRLVPSYSKGNPHIPDETYQRVYEKWLKG"
     gene            1338..4139
                     /locus_tag="PLF14_07675"
     CDS             1338..4139
                     /locus_tag="PLF14_07675"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOT71202.1"
                     /translation="MNAESRFRKKKEMLKRGLGKIKSTGNLVFTVLLLVVILFGLMFF
                     YFEFETITGEYEVKLETMAYALSSGIPKEVLDRYNGDGSYADDKLYREVTENIKTYVA
                     KNEDIVGVSLFVIREGQLIPFIQTGDINNHVSKNCIHEVIKSALDNNTKVSYVPDFSD
                     IPEKSACIAVPVAGGGSGGFLALETSTAVLYEQIKSQHLISVSMFVLIVILYIIVGIT
                     FMRSGSLREDKKRISESVNDLKEKEMLFRSVFEQAAPGISINSVDEELDTVPDSVNVN
                     DSFKRITGYTLEQLKKISWTEMTHSDFVDEERKMMQDFLQGNIDGYQMEKKIVRADGT
                     ESWINIAVSALYPDNQQDHKYLCILEDIGNRKKMESSLAESEKDKAILISNVPGMLFR
                     IGCEKNWTLQFVSSGCLELTGYPADRFMGKEGIIFGQLIIPEYSEYVYKKVQQAVADR
                     ARFSIEYEISTASGERKWISNQGQIVCGTDGKPLYVEGLAIDIDESKKRELELRYINE
                     HHPLTDLYSRSYFSFLLNKELEEGIVDKKAAIFINIRNFSLLNATYGYAAAEEIIKEL
                     AEQLRLLCTDNCRIFHISIDRFVLYVKGYEGKQELVRMCNKITAMLYGRFATRLVGAS
                     IGVYEFESHMADGEIVIRNASIAAEHTDRTKVFSFMFFNADMEKKIMRSNIIEEELAE
                     VSSGTEKEKLYAVFQPIVRLSDDSIAGFEALARFNSDILGNVPPNEFIPIAEEKQYIF
                     GLSRSIIRQSLEFVNELSKKGADDVFVSVNISVIQLLHEDFVADIVNLVREYSVNPDK
                     LVLEITETKVARNYAKINEKLSLLQNEGFKIAMDDFGTGYSSLARESEMNVNYVKIDK
                     YFMDKLLVYDENQAVTSDIISMSHKLGYYIVAEGVEYTEQKEYLKKYYCDYMQGYLFS
                     RPISAKDALLLVKKHRARA"
     gene            complement(4165..4770)
                     /locus_tag="PLF14_07680"
     CDS             complement(4165..4770)
                     /locus_tag="PLF14_07680"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOT71203.1"
                     /translation="MFCTNCGNRIEPGQLFCTSCGTRVAGEVQNTVNYSTPQTHASYG
                     VVRVLTAQKKLSMFNMITCYVVLFNDRLVLAHITPEFQKAESARKSAEIRASGTGFFK
                     GSAEMMRFWSYYHKKYETMSPPAILAECPMNMEIPYNMISQLLFRAYEEGDEDSSSSG
                     GDLNISLSNGNVIKLKHKHDHSKALNNDLQSLLGFRLKYKK"
     gene            4912..6303
                     /locus_tag="PLF14_07685"
     CDS             4912..6303
                     /locus_tag="PLF14_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT71204.1"
                     /translation="MFIGRERELNTLNKLYNSGKFEFAVIYGRRRIGKTALISEFVKK
                     KDTIFFTGVETNAKQNLDNFSGCIMEYNTGMPIDWSFNSFQSALEYVFELARSKRIIL
                     VIDEYPYVARSSKSLASTLQYLIDKNKDNSKLFLILCGSSMSYMEDNVLAYKAPLYGR
                     RTAQLKIEPFDFFETCHYFSNLSDIDKALAYGVIGGTPQYVLQFDDNISIKENILNTY
                     LNPTSFIFEEPNNLLKQEMREPAIYNAVISAIAAGNSKISEISGKIGETTSLSATYIR
                     NLIILGIVKKEWPYGEKSGRKTIYSIEDNMFRFWYRFVPQNISVISRGAASLAYERIE
                     PELSSYMGSVFEEICKQYLWKLLINGKCAVNFSDIGRWWGTNPKTRVQEEIDIMGSYK
                     DSALFAECKWTNERTDVGVLETLIERSNLFDYEKKHFYLFAKTGFTKGCHDYAEKMGN
                     VTLVTYEDILKST"
     gene            complement(6328..7122)
                     /locus_tag="PLF14_07690"
     CDS             complement(6328..7122)
                     /locus_tag="PLF14_07690"
                     /inference="COORDINATES: protein motif:HMM:NF020227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT71205.1"
                     /translation="METKVAVITGASSGIGLATALKLVNKGYTVYNLSRRNFVHDRIR
                     HVTCDVSSALSVNNAMAQVIKQCGKISLLVTSAGFGVAGAVEFISDEDAKKQMDVNYF
                     GTVNAVKAVIPYMRNYKSGKIICISSVAAAIPIPFQTYYSVSKSAINAFVSALAHEIK
                     PFGISVCAIMPGDIKTGFTSARKTDLTGNEIYGGRIKKSVAVMEKDEMNGLPPDVVAD
                     FIVRLAKKRKLKQIYTVGFVYKLFVLLSKILPQRIVSAIIYMLYAK"
     gene            complement(7122..8300)
                     /locus_tag="PLF14_07695"
     CDS             complement(7122..8300)
                     /locus_tag="PLF14_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT71206.1"
                     /translation="MDIFNKCYNYTKADEVRELGIYPYFHSLQSKQDTVVIMEGKRRI
                     MLGSNNYLGLTTHPDIMKAGIEAVERYGTGCSGSRFLNGTLQLHLELEKELADFLHQQ
                     SALVFSTGFQTNLGIISAIAGHNDYLVCDKENHASIYDGCRLSQGTLLRYRHNDMSDL
                     ERQLKKIPETAGILIIVDGVFSMGGDIADLPAIVDLAKKYGARVMVDDSHALGVIGEG
                     GRGTASYFNLEKEVDIYMGTFSKSLASLGGFMAADEKIIDYVRHNSRPFIFSASMTPS
                     GCAVALAALRHLRAHPELPKQLTDIASYFRKRLKEEGIKTRDSLTPIIPIYTYEMITT
                     LTIAKELYDEGVYVNCSLPPATAPNECLLRTSLMATHTKELIDEAVMSIKKVLGRYNL
                     "
     gene            complement(8538..9191)
                     /locus_tag="PLF14_07700"
     CDS             complement(8538..9191)
                     /locus_tag="PLF14_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008402249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOT71207.1"
                     /translation="MSEISNLFPVWYHLTEEQKQLLNDKVYIKDVKKGTVLHRGDEDC
                     DGLYIVRTGQLRAYIISDQGKELTLYRLFEMDICLFSASCILSNIQFEIIVEAEKDTS
                     ILIIPVRVYQKLMETNNAVANYTNHLMASRFTDVMWLIDQVLFKSMATRLASFLLYES
                     NIEESDELNITHEKIANHIGTAREVVTRMLKYFQQEDLIKMSRGSIIITDRKKLENV"
     gene            9416..11302
                     /locus_tag="PLF14_07705"
     CDS             9416..11302
                     /locus_tag="PLF14_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009326189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOT71208.1"
                     /translation="MDFKKRAAELVSKMTLAEKMSQMQFESPAIERLGIPEYCWWNEA
                     LHGVARSGTATVFPQSIAMAASFNDRLMYKVATAISDEVRAKYNQYRKLNGTKIYEGL
                     TCWSPNINIFRDPRWGRGHETYGEDPLLTAKMGTAFVKGLQGNDKIYRKTDATLKHYA
                     VHSGPEWLRHSFDAHVSETDFYETYLYAFEYCIKHAKPAAVMGAYNRVYGEPCCGSPF
                     LLQKILRKKLGFKGYVVSDCGAICDFHLHHKVTENEAQSAALAVKSGCDLNCGDAYKW
                     LKVAVAEGLVKEKDITKCVERLMYTRLKLGMFDKECKYNDIPYSVICSKKHKKLNLEM
                     ARESIVLLKNNGILPLKKNTNVAVVGPNADDKSVLLANYNGTPDDYYTLLRGIQSVCK
                     GDVLYARGSHVFEDESTWRYSEHPLREAIITAGYSDVIIMCMGINPSIEGEESTHRGF
                     KGDRDEIELPAVQYKLFDELKKLGKPIIFVNVSGSCVGLAHADKNADAVIQCFYPGAL
                     GGIALADVIFGNVSPCGRLPVTFYEKTSDLPDFEDYSMKGRTYRFFEGQPVYRFGHGL
                     TYANIKEEWIDDYTVKLTNPSDVDTGYSVLRYEDKTKRKLLDFKKVQLKGNSSITVQF
                     TPGK"
     gene            11349..12944
                     /locus_tag="PLF14_07710"
     CDS             11349..12944
                     /locus_tag="PLF14_07710"
                     /inference="COORDINATES: protein motif:HMM:NF025067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HOT71209.1"
                     /translation="MKKIFELILSNIKEAMSKFVVSFCGSVIAFILLTLQIIFDFTEN
                     TVVLMAMSVGFAVIVSVMMQIISIKYNFSKKADIIQRIVSALTAVPCYFLIGNIMESD
                     RTFMGFFGLIIAFLLISLYVMYDNENSDLVFPQIIKSAFFAVTVASLFFGGITLCIAA
                     LDFLVVELEQFDKWIIITAMLSYIVLCYNLFIAMLPEKTDEITLPKIFKVLVLYVGLP
                     VYTLLLGVLYAYLIKILITMEMPQGKINLFASLASLFFIFFYLTVGSYDNAPVRFFRK
                     YGGWFLFPIIVAQAIAIYIRVNAYGLTSARWASILLNVTALAFIILTLIKEGKYTKHV
                     ITGLAVIVLISSLTPLNIIDVPVYEQTYRLEKVLKRNNMLSDGVVKPNKDISYADKEI
                     ISSCYDYLNYKDKAPEYVRYTNSFYDIFGFEKLNEYGSLDEYSLIHLNKSNSYNALDI
                     SGYSRYYIVDTSRERELKIVIDNTEYNIAEHIMKIKEGNDRSDMVFDITEDIRVYLTY
                     VYYEMNNEGEVLYFSATGFALGK"
     gene            13026..13931
                     /locus_tag="PLF14_07715"
     CDS             13026..13931
                     /locus_tag="PLF14_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006635292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HOT71210.1"
                     /translation="MGRYRLITRSDFDGLVCAMILKELDMLEDIMFVHPKDMQDGMID
                     VNEKDISTNVPYLPGVYLAFDHHISEKGTKAVNYINKPDAASTARVLYEYFGGASRLK
                     ISEELMAAVDKFDSAQYTMDDIVNPKGWVMLSYLLDARTGLGRFRNFRITNYDLMMML
                     LDYCPDHTIDEIMRLPDVQERVKMYYEHEKLFRDQLKGCAEVVKDVVILHLKNCDPIY
                     VGNRFYVYTLFPQATVSIHEMWGLKKRNTVFTLGKSIINRGSDKDLAAICKKHGGGGL
                     MNAATCQVPNGLADGILEDILQQLL"
     gene            13945..14262
                     /locus_tag="PLF14_07720"
     CDS             13945..14262
                     /locus_tag="PLF14_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019554844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HOT71211.1"
                     /translation="MERYAWRAKVKEGMLDEYIRRHDEIWPEMVKLLKKAGIKNYTIW
                     YNNNDLFGYYECEHGVEYAAKVQAESPIVDKWNEYMKDVMVMEMDPVTKAQPLMRKVF
                     FLE"
     gene            complement(14323..15723)
                     /locus_tag="PLF14_07725"
     CDS             complement(14323..15723)
                     /locus_tag="PLF14_07725"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOT71212.1"
                     /translation="MKKPNILLFLADQHRYDCVGCSNIRAVRTPNIDRLAKEGIVFDN
                     AYCPTPVCAPARQSFMNGMRAESIGALWNFGFVPTNTVQPTDWNIPKSLKDAGYENIF
                     IGAWDVSRIPPEQYGFNVTHLSKQYNKYISEKYPNVQYTNSWFGEVNPVPYSDSRPKY
                     YADRVCEQLDRLKDGDKPWFIWVDGVDPHLPCRPCKEFYDMYAPEDIPPFGGFGDTFK
                     NKPYIQKQQILNWRLENMSWEDWSETVRCYYAMITQIDHSFGIISDKLKECGMYDDTL
                     VIYTSDHGDMCGSHGMIDKHYIMYDDVLRIPMIMSYPKLFKNNSVDYRRCDTFVYNAL
                     DIPATICELCKIERNPDCHGRSLLSILKGEKPNKEQFPDYTVSASNGQQFGSFTQRSI
                     RTDKYKYIWNLTDIDEFYNLQTDPYELENLIYDSSYTDIISDLRKLLKKELARCSDPF
                     VKSGWLNRTLEENIKL"
     gene            complement(15723..16832)
                     /locus_tag="PLF14_07730"
     CDS             complement(15723..16832)
                     /locus_tag="PLF14_07730"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT71213.1"
                     /translation="MAIKIGIVGMGGIGNTHARCYKADPLAQLVAVCDVIKEKADKAA
                     NEHNVQAFYCIEDMMKAHPELDLIDVTTSGFDNGSWHYEPVMKALDAGFNVMVEKPIS
                     NDNDEAREMVVTAQKKNLYLGCNLNHGFTGPADKAKELISQGKIGAPVYILHKVGFNG
                     SEYSYKPLPKDGPMRRFAGPYSHAYAFLSHPFSLMRSFGGDITHVQAFFDKPGGRKSA
                     NDLMWSINGINVKYQNGSIGYILSQRGDAIFGLGGWWSFEMAGTKGTFCIENCVEKLY
                     YWDGSKPASMGTMQAEVFDFGTTDFGITFNNRLHAFLEDMTNKVPPEHIRGSGRDALA
                     TLEYTVAAIKSFEEGGALVRPRALPPLHGTPGFVV"
     gene            complement(16844..17854)
                     /locus_tag="PLF14_07735"
     CDS             complement(16844..17854)
                     /locus_tag="PLF14_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT71214.1"
                     /translation="MLRTAVIGMGPIGNNHAKAYKSCKQAELVAVCDIRKDRADAAKE
                     RYVVPAYYDAKEMLKAEKPDIVSVATGGFEYSSDHYIPTMQALNAGCHVLTEKPISNN
                     LQHGQEMVDLAKKLDRCFGIDLNHRFTPAARIAKKWQNEGLIGNLLFLNMALWIGRFQ
                     PHFDTEYYHLKALNPHSCDILRYFGGDVEQVHCYAMKAPGREIWSTASINMKFVNGAV
                     GHLTSSYDIERGHPMERCEVAGTKGRLIFEDMWREATLYPAGNYEKRVYTNPVFGGFR
                     DFYDTFEDRIHTFVREVAEGVKPEDIDGSGKDGLEASRIIHAAIQSLKTNKPVIVANI
                     KE"
     gene            complement(17866..19245)
                     /locus_tag="PLF14_07740"
     CDS             complement(17866..19245)
                     /locus_tag="PLF14_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010151972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOT71215.1"
                     /translation="MAKKPNLLFFGIDSLRRDHMSLYGYNRLTTPNIDKYLKKGTVYN
                     NCFSPHIPTTPGYANMLTGFDCFGTDIVALRHKGDIAPGVKTLAEILSSHGYTTTNIG
                     FKNVSGRGFDNYIQYSGWSIEEHDKRAHKAESMNAVAIPELERLAAQDKPFFLFLRHM
                     DPHSPYLPPGPWERMFYQGNEFDPNNRSMEPVMAFKPFCDYFAEWIPEGCTDKDYVIA
                     QYDAAIAYMDTCIQSILQKLYDLGLENETLVVFTSDHGETLYDHDCYFDHHSLYDPTL
                     VVPLAFRFPGRVPEGVRVDDTCRLYDVTPTVLDILGIETRINFDGRSVFPLAFGQPIP
                     KLDEFYITECTWMRKHGWRTPKWKYIQALEPDFHFKPEVELYDLENDPMELWNIAKDR
                     PDIVMMLDKKMSEFIRKREKEVGRTNPIYTNLTWHGLDLNRGFISSEEAYNSMHIASA
                     KTAAKLQQK"
     gene            complement(19326..20123)
                     /locus_tag="PLF14_07745"
     CDS             complement(19326..20123)
                     /locus_tag="PLF14_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOT71216.1"
                     /translation="MIKLGVNSVLFKHYTFGQAAEAIAKCGYDGVEISAIPGMCEHLQ
                     VENWRAQKREIKAILEATGLTILASEVASRDKKRMKKALAAAEYFGIPVLNVGPGGKA
                     GNEEDLIETARSLSELAKMAEKYGVTICCKAHVGASMHNTETTLRVMQMVDSPNFGVD
                     MDPSHIYRASELPEEAIAAVLPAMKHIHIRDCKGRGPGPGHPSLQACGKGDIYLFGYI
                     SKMVEAGYSGPVNLEVIGPEQDMVEATRIAAESYGYINACLKILNAR"
BASE COUNT         5840 a         4032 c         4457 g         6088 t
ORIGIN      
        1 aaaaaggagt tgggtgagga tattgcctac tatacctcct ttgacgactt tataaaccat
       61 gacatggatg ctgtatttct tgccaactat gcaacggaac atgcaccgta tgcaatacgt
      121 gcttttgaaa agggtctgca tgtattgagt gaagtgttgc cctgtcagac aatgaaagag
      181 gctgttgaac tgatagaagc catagaaaaa agcgggaaga tatatgcata tgcggagaac
      241 tgctgtttta tggctgcacc ttacgagatg cggaaacttt atcgttcggg tcaattggga
      301 gaattggaat acggagaagg tgagtatttg cacaattgcg aatccatatg gccgagaatt
      361 acccgaggag ataaaaacca ttggcgtaat aacatgtatg ctcactttta ttgcactcac
      421 tcattcggtc cgtttattca tataaccggt cttcgcccga tatcggtaac gggttttgag
      481 ttacctttta atgcaagaat ggaaaggatg ggtgccaaat caggcagcgc cggtttggca
      541 attataacta tggagaacgg agcaatcatt aaaaacatta acggtgtcgg atgttccaaa
      601 aattctcttt ggttcagtat atacggaaca ctgggcagag cggaaagtgc cagagaggat
      661 agtcagtatg gtatgtatag ccgtattttc accaatcttg acagatatga gggagagtgt
      721 ttcgacaggc cgaatactta ttgtcccgga gcggatcaac cttcaccggc tcaaaaatgg
      781 ggacacggag gcggcgatta ctatatcatg cacaactttg ttgaccgtat ttcgggaaat
      841 acggatgcgg agactattga tatatatgaa gcgttggata tgttccttcc cggtatgttt
      901 gcttatcgat cggtgttggc aggaggaata cctgtgaata ttccaaatct tcggatacct
      961 tccgagagag acagatggcg taatgataca tggtgtaccg atcccgccgt tgcaaaagac
     1021 cggcttgtgc cgtcatattc caagggaaat cctcatatcc ccgatgaaac atatcagcgt
     1081 gtttatgaaa aatggttaaa agggtaactt tttttgctgt aaagagttgt atttttatgc
     1141 actttgtatg ataatattac cagttcctaa aaaaaccttg ttgtaacttc tacagtgttg
     1201 tggtgctgta aaggtccggg gtaggcagat tattgagtat atattgccat gattgcgtca
     1261 gagaactgca gtgtgcaaaa gattattatg tcttttaatt gcaggtaact tgtttaaagg
     1321 aaacaacggg taacaacatg aatgctgaat cgagattccg gaaaaagaaa gagatgctaa
     1381 aaagagggtt aggtaaaata aaaagcaccg ggaaccttgt ctttacggtg ttacttcttg
     1441 tagtaatctt attcgggctt atgttctttt attttgaatt tgagactata acaggtgagt
     1501 atgaggtcaa actggagact atggcttatg cgctttcctc aggtatacct aaggaggttc
     1561 ttgacaggta taacggggac ggttcttatg cggacgacaa gttatacagg gaagtgaccg
     1621 aaaacatcaa aacatatgta gcaaaaaacg aggatattgt aggtgtttca ctttttgtca
     1681 tacgggaggg acaattaatt ccttttatac aaacaggtga tataaataat catgtatcga
     1741 aaaattgcat acatgaagtt attaaatctg cccttgataa taatacaaaa gtttcctatg
     1801 tgcccgattt ttccgatatc cccgaaaaaa gtgcatgtat tgcggtgccg gttgcgggtg
     1861 gcggttccgg gggttttctt gcgttggaaa catctacagc tgtactctat gagcagatta
     1921 agagtcagca ccttatatcg gtatccatgt ttgttctgat tgtcatcctt tacattattg
     1981 tgggaattac ttttatgagg agcggttctc ttcgtgagga taagaaaaga atatcggaat
     2041 cggtaaatga tttaaaagaa aaagaaatgc ttttcagaag tgtttttgaa caggctgctc
     2101 cggggatatc cattaacagc gtagatgagg aacttgatac cgttcccgat tcggtaaatg
     2161 tcaacgactc atttaaaaga atcaccggat atacgctgga gcaactgaag aagatcagtt
     2221 ggacggaaat gactcacagt gattttgtag atgaagaacg aaaaatgatg caggactttt
     2281 tgcaggggaa tatagacgga tatcagatgg aaaagaaaat agtaagagcg gacggaacgg
     2341 aaagctggat taatatagcc gtatcggcac tgtatccgga taatcagcaa gatcataaat
     2401 atctatgtat attggaagat atcggcaatc ggaaaaagat ggaatcttca ctggcggaga
     2461 gtgaaaagga taaggctatt ttaatatcca atgtgccggg tatgttattc agaatcggat
     2521 gcgagaagaa ttggactctg caatttgtat cttccggctg cctggaactt acgggttacc
     2581 ctgcagacag atttatggga aaggaaggca ttattttcgg tcagttgata ataccggaat
     2641 attccgaata tgtgtataaa aaggtgcaac aggccgttgc ggacagagca agattcagta
     2701 ttgaatatga gataagtacc gcatccggag agagaaaatg gatatccaat caggggcaga
     2761 ttgtatgcgg tacggacggt aagccgttat atgtagaagg gctggctatt gatattgatg
     2821 aaagtaagaa gcgggaactg gaactgagat atataaacga acaccacccg ttgaccgact
     2881 tatacagcag aagttatttc agctttttac ttaacaagga actggaagaa ggtattgtgg
     2941 ataaaaaagc cgcaatattc ataaatatcc gcaacttcag tcttctcaat gctacatacg
     3001 gttatgcggc tgcggaagag ataattaaag aacttgcgga gcagttaagg ctgttatgta
     3061 ccgataactg tagaattttc catatatcca tagacaggtt tgtattgtat gtaaaaggat
     3121 atgaaggaaa acaggaactt gtaagaatgt gcaacaagat taccgccatg ttgtacgggc
     3181 gatttgcaac caggttggtc ggagcaagta tcggagtata tgaattcgaa tcacacatgg
     3241 cggatggaga gatagttata aggaatgcat caattgccgc ggaacatacc gacagaacca
     3301 aagttttctc ctttatgttt ttcaatgccg atatggaaaa gaaaataatg aggagtaata
     3361 tcattgaaga ggaactggcg gaagtatcat ccggaaccga aaaggaaaaa ttatatgcgg
     3421 tatttcaacc tattgtcaga ttgtcggatg acagcatagc cgggtttgaa gcccttgcaa
     3481 gatttaattc tgatatactg ggcaatgtgc ctcccaatga attcattccg atagcagaag
     3541 aaaaacagta tattttcggt ttgagcagaa gtataatcag acagtcactg gagtttgtta
     3601 atgaactgag taaaaaagga gcggatgatg tgtttgtatc ggtaaatatt tcggtcatac
     3661 agctgctcca cgaggatttt gtagccgata tagttaatct tgtccgggaa tattcggtaa
     3721 atcccgataa acttgtactg gagattaccg aaacaaaagt tgccaggaat tatgctaaaa
     3781 taaatgaaaa actctctttg cttcagaatg agggctttaa aattgctatg gatgatttcg
     3841 gtacaggtta ctcttctttg gcaagagaaa gcgaaatgaa tgtcaattat gtaaaaattg
     3901 ataagtactt tatggataaa cttcttgtgt atgacgaaaa tcaggcggtt acatcggata
     3961 ttatttccat gtcgcacaag ttgggatatt acattgtagc ggaaggggtg gaatacaccg
     4021 agcagaaaga atatcttaag aaatactact gcgactatat gcagggatac ctgttctccc
     4081 gaccgatctc cgcaaaagat gcattgttat tggtaaaaaa acaccgtgct cgggcataac
     4141 acttattcca tgttattgcc gtatttattt cttatacttc aacctgaaac ctaaaagcga
     4201 ttgcaaatcg ttatttaatg cttttgaatg gtcgtgttta tgcttcagct taatgacatt
     4261 gccgttactc aaagatatat tcagatctcc tccggaagag gaactgtctt catcaccttc
     4321 ttcgtaagcc ctgaaaagca actggcttat catgttatag ggaatctcca tattcatggg
     4381 gcattcagcc aaaatagcgg gcggggacat ggtctcgtat tttttgtggt aataagacca
     4441 gaatctcatc atttcggcgc tgcccttgaa gaaacctgtt ccgcttgctc ttatctcagc
     4501 ggattttctg gcactctcgg ctttttgaaa ctcaggtgtg atatgcgcaa gaaccagcct
     4561 gtcgttaaac agaacgacat agcatgtaat catattgaac atggacagtt ttttctgagc
     4621 cgtcagaacc cttacgacgc cgtaagaagc atgtgtctga ggggtgctgt aattgacggt
     4681 attctgaact tctccggcta ctcttgtgcc gcaactcgta cagaacagtt gccccggttc
     4741 aatcctgttg ccacagttag tacaaaacat attaattacc tccgaaatat atactgatta
     4801 tatacataag acggaggaat ttcaataaat cataaatagt atttacattt gctgtttggt
     4861 ggtttataat taagtaaatc ataaacaact aaattacaag ggagtgcacg gatgtttata
     4921 ggaagagaac gggaactaaa tacgcttaat aagctatata actcggggaa atttgaattt
     4981 gccgttattt acggacgtcg ccgaataggc aagaccgctc ttataagtga atttgtaaag
     5041 aaaaaagata caattttctt tacaggagtg gaaacaaacg ccaagcagaa tctggataat
     5101 ttctcaggat gtattatgga atataacacc ggaatgccga ttgattggtc ttttaattcc
     5161 tttcaatctg cattggagta tgtttttgaa ctggcaagaa gcaaacggat tattcttgtc
     5221 atagatgagt acccctatgt ggctcgttca tcaaaaagcc ttgcttccac gttacagtat
     5281 ttgatagata agaataaaga taattcaaag ttattcttaa tactatgcgg ttcttccatg
     5341 tcatatatgg aggacaatgt tttggcatac aaggcacctc tttacggaag aagaactgct
     5401 cagttaaaaa tagagccgtt tgactttttt gaaacctgtc attatttttc aaacctgtca
     5461 gatattgata aagctctggc atacggtgtt ataggcggaa ctccgcaata tgtgttgcaa
     5521 tttgatgata atatttccat aaaagagaac atcctgaata cttatctcaa tccgacttct
     5581 tttatttttg aagaaccgaa caaccttctg aagcaggaaa tgcgtgagcc tgcaatatac
     5641 aatgccgtta tttcggctat agccgccggt aactcaaaaa taagtgaaat ttccggcaag
     5701 atcggtgaga ctacaagttt atctgctact tacatcagaa acctcataat tctgggtatt
     5761 gtaaaaaagg agtggcctta cggtgaaaaa tccggtcgta agaccatata ctcaatcgag
     5821 gataatatgt tccgtttttg gtatcgattt gtgccgcaaa atatttccgt tatctcccgt
     5881 ggagcagcct cccttgctta tgaacgaatt gaacccgagc tttcatccta catggggagt
     5941 gtatttgaag aaatctgcaa acagtacctt tggaaattac tgattaacgg caaatgtgcc
     6001 gtgaatttca gtgacatcgg gcgttggtgg ggaacaaatc ccaagaccag agtacaagag
     6061 gaaatagata ttatgggctc atacaaagat tcagccttat ttgccgaatg taagtggaca
     6121 aatgaaagaa cagacgtagg tgtgctggag acactgatag agcgcagtaa cctatttgac
     6181 tatgaaaaaa agcactttta tctgtttgcg aaaacaggat ttacaaaagg ctgtcatgac
     6241 tatgccgaaa agatgggtaa tgtgactctt gtcacatatg aagatatact gaaaagtaca
     6301 taatagaagt ataaatgcag ttaaagatta tttcgcatac agcatgtata ttattgcact
     6361 tactattctc tgaggcagta ttttggataa caggacaaac agtttatata caaatccgac
     6421 cgtgtaaatc tgcttaagtt ttcttttctt tgccagtctt actatgaaat cggccacaac
     6481 atccggaggc aaaccgttca tttcatcctt ttccattacc gcaacggact tttttatcct
     6541 accgccatat atttcattac cggtaaggtc ggtttttctt gcggatgtga aacccgtctt
     6601 tatatcaccc ggcattattg cgcatacgga aattccgaaa ggctttatct catgagccag
     6661 agcggacaca aatgcgttta ttgcggattt ggataccgag taatatgtct gaaaggggat
     6721 agggatggct gccgctaccg atgatatgca tattatcttt ccggatttgt aatttctcat
     6781 gtacggaata accgctttta cggcattaac cgtgccgaaa tagtttacat ccatctgttt
     6841 ttttgcatct tcatcggaaa taaattccac ggctcccgca acaccgaaac cggcacttgt
     6901 cacaagcaga cttatcttac cgcattgctt tatcacctgt gccattgcat tgtttaccga
     6961 taaggccgaa gagacatcac aggtcacatg tcttatccgg tcatgaacaa aattccttct
     7021 gctgaggttg tataccgtat accctttatt taccaatttt aatgctgtcg caagaccgat
     7081 accgctgctt gcaccggtta ttacggctac ctttgtttcc attacagatt ataccttccc
     7141 agtacttttt ttatgctcat aaccgcctcg tctataagtt cctttgtatg agtagccata
     7201 aggctggttc tcaacagaca ctcgttggga gcggttgcgg gcggaagcga gcagtttaca
     7261 tatacgcctt catcatataa ttcttttgct attgtcaatg tggttatcat ctcatatgta
     7321 tagatgggta tgatgggtgt aagcgaatca cgcgtcttta taccttcttc tttcagtcgc
     7381 tttctgaaat agcttgctat atcggtcagt tgtttcggca actcgggatg agctcttaaa
     7441 tgtctcagag cagccaatgc gaccgcacaa ccggaaggag tcatggatgc cgagaaaatg
     7501 aacggtctgg aattatggcg tacatagtca atgatttttt cgtctgcagc cataaaaccg
     7561 cctaagcttg ccaaggactt ggaaaaagtg cccatataaa tatccacttc tttctccaga
     7621 ttgaaataac tggcggttcc tctgccgcct tctcctatta ccccaagggc atgtgaatca
     7681 tccaccatta ctcttgcacc gtattttttg gcaaggtcca ctattgcggg aagatcggct
     7741 atatcgcctc ccatggaaaa gactccgtct actattatga gaattcctgc ggtttcgggt
     7801 atttttttaa gctgcctttc cagatccgac atatcattat gtctgtatct taataatgtt
     7861 ccctgcgaca atctgcaacc gtcatatata gatgcatgat tttccttgtc gcatacaaga
     7921 taatcgttat gaccggcaat tgccgagatg attcctaaat tggtctggaa tcccgtagag
     7981 aaaaccagtg cggattgctg atgtaagaaa tcggcaagtt ccttttccag ttccagatgc
     8041 agttgcaatg taccgtttaa aaatcgcgaa ccggagcatc ccgtaccgta tctttctacg
     8101 gcttctattc cggctttcat aatatcaggg tgggtagtca gtcccagata gttattggaa
     8161 cccagcatta tcctgcgttt tccttccatt atgactactg tatcctgttt tgattgtaaa
     8221 gaatgaaaat acggatatat gcccaattca cgcacttcat ccgcttttgt gtagttatag
     8281 cacttgttga aaatatccat attactacct ccgtaaaacc cggtaccatt gcatatagca
     8341 atgactgatg attgtttacc gatcaatgtt ccccccattt tttgatataa ctcatcagta
     8401 tttattacca agtaataatt tatctgtaat tatataaata acatgcatat ttgtcaacat
     8461 tcagcatgta tccggtaagg gttttatagg tgaaagagaa agaatttact ccgaatttac
     8521 cgaaagaaac taatttttta cacattctcc aatttctttc tgtcggttat gatgatactg
     8581 cctctggaca ttttgattaa atcttcctgc tggaaatatt tcagcatcct ggttacaact
     8641 tctctggcgg tgcctatgtg gttagctatt ttctcatgcg taatattcaa ctcgtcactt
     8701 tcctctatat tgctttcata cagaaggaaa gaagcaagac gtgttgccat gcttttgaaa
     8761 agaacctgat ctatcaacca catgacatcg gtaaatctgg aagccataag gtggttggta
     8821 tagtttgcaa ccgcattatt tgtttccatg agtttctgat atacccgaac cggtattatc
     8881 aatatggagg tatctttttc cgcttccact ataatttcga attgtatgtt ggacagtata
     8941 caggatgcgg aaaacagaca tatatccatt tcaaataacc gatagagagt aagttcctta
     9001 ccctggtcgg aaatgatata tgctcttaac tgaccggttc gtactatgta taatccgtca
     9061 cagtcttcat cgcctctatg aagaacggtg ccttttttaa catcctttat atacacctta
     9121 tcgtttaaca actgtttctg ctcctcggta agatggtacc agacggggaa caggttactt
     9181 atttcactca tgataactcc ctgatgcatt cttatacgtt cacattccga ttataccata
     9241 cacgcatgta aagttttatt tataatatta tttatttata aataagaatt aacaaaacta
     9301 tgttatatat acaggtttta attgtgttat tcttctgata taataactgc atcggttgcc
     9361 tttgcaacgt taattttaat aattcataaa tcttgttttg tataaggagg aacagatgga
     9421 ttttaagaaa agagctgctg aacttgtaag caagatgact ttggcggaaa aaatgtcaca
     9481 gatgcagttt gagtcacctg caatcgaaag attgggcatc cccgaatact gctggtggaa
     9541 tgaagcgtta cacggagttg ccagaagcgg aacggcaacg gtatttcctc agtccatagc
     9601 aatggccgca tcttttaacg atagactgat gtataaagtg gcaacggcaa tatccgatga
     9661 agtgcgcgcc aaatacaatc aatacagaaa attgaacggc accaagatat atgaaggact
     9721 gacctgctgg tcgcccaata tcaatatatt ccgtgatccc aggtggggaa gagggcatga
     9781 gacatacgga gaagaccctc tgcttaccgc taaaatgggc accgcctttg tcaaaggtct
     9841 gcagggcaac gataaaatat acagaaagac cgatgccacg cttaaacatt atgcggttca
     9901 cagcggtccc gagtggttac gtcacagttt cgatgcacat gtatcggaga cggattttta
     9961 cgaaacatat ctgtatgctt ttgaatactg cattaaacat gccaaaccgg ctgcggttat
    10021 gggagcatat aacagggtgt acggagagcc gtgttgcggc agtccgttcc ttttacagaa
    10081 gattttgcgt aagaaattag gctttaaagg atatgtggta tccgactgcg gagcaatatg
    10141 cgatttccat ctgcatcata aggtaacgga aaatgaagcg caaagtgcgg cattggcggt
    10201 aaagagcgga tgcgatttaa actgcggtga tgcatataaa tggcttaaag tagcggtagc
    10261 cgaagggctt gttaaggaaa aagatattac aaaatgtgtg gagagactga tgtatacgcg
    10321 tctcaaactg ggtatgttcg ataaagaatg taagtataac gatattcctt attcggtaat
    10381 atgcagtaaa aaacataaga aattaaatct tgaaatggcg agagaaagta ttgtactgtt
    10441 gaagaataac ggaatactgc ctttgaaaaa gaatactaat gttgctgtag tagggcccaa
    10501 tgccgacgac aaatcggtac ttttagccaa ttacaacggc acgcccgatg actattatac
    10561 attgcttcga ggaattcaat cggtatgcaa aggcgatgtt ctttatgcaa ggggctcgca
    10621 tgttttcgaa gacgaatcca catggagata ttccgaacat ccgctacgtg aggcgataat
    10681 aacggccggt tacagcgatg taatcatcat gtgcatgggt atcaatccct cgatagaagg
    10741 agaagaatca acacacagag gattcaaggg agacagagac gaaatagagt tgccggcggt
    10801 acagtataaa ctgtttgacg agctgaagaa actgggaaaa cccatcattt tcgtaaatgt
    10861 atcgggcagt tgcgtaggat tggcacatgc cgataaaaat gccgatgccg ttatacagtg
    10921 tttctatccg ggagcattgg gaggaatagc tcttgccgat gtgatattcg gaaatgtatc
    10981 tccctgcgga aggttgcccg ttactttcta cgaaaagaca tcggatcttc ccgattttga
    11041 agattattcc atgaagggaa gaacctacag attctttgaa gggcagccgg tataccggtt
    11101 cggacacggt cttacatatg caaatataaa agaagagtgg attgatgact ataccgtaaa
    11161 actgacaaat ccgagtgatg tcgataccgg ttattcggtg ttgagatatg aagataagac
    11221 aaagaggaaa cttttagact ttaaaaaagt ccaattaaag ggaaacagca gtattactgt
    11281 gcagtttaca cccggtaaat aacagaaagg actgatactt atcagagtat cggacgagga
    11341 gcggtgttat gaagaaaatt tttgaactga tactgagtaa tattaaagaa gctatgtcga
    11401 aattcgtggt gtctttttgc ggaagcgtta tcgcttttat acttctgaca ttacagataa
    11461 tattcgactt cacggaaaat accgtagtgc ttatggctat gtccgtgggc tttgccgtca
    11521 tagtgtctgt aatgatgcag attataagca taaagtacaa tttttccaaa aaagccgata
    11581 tcatacagcg cattgtttct gcattgaccg ctgtaccgtg ctattttctt atcggcaata
    11641 taatggagtc ggataggact tttatggggt ttttcgggct gataatagca tttctattga
    11701 tatcgttata cgtgatgtat gataacgaga atagtgatct ggtctttcct cagattataa
    11761 aatcggcatt ctttgcggta acggtggctt cactgttttt cggaggcata acgctgtgta
    11821 tagcggcatt ggatttctta gtggtagagt tggagcagtt tgacaaatgg attatcatta
    11881 ccgccatgct gtcatatatc gttctctgct ataacctgtt cattgctatg ttgccggaaa
    11941 agaccgatga aataactctt cccaagatat tcaaggttct ggtgctgtat gtcggtcttc
    12001 ccgtatacac gctgctgctc ggagtgctct atgcatatct tattaagata cttataacca
    12061 tggagatgcc gcagggtaag attaatcttt ttgcttcgct ggcttctctt ttctttattt
    12121 tcttctacct taccgtagga agttacgata atgcgcctgt gcggtttttc agaaaatacg
    12181 gaggatggtt cctgttcccg ataattgtgg cacaggctat agcgatatat ataagggtaa
    12241 acgcatacgg actgacctct gccagatggg catcaatact gctgaatgta acggctctgg
    12301 cctttataat ccttaccctt atcaaagagg gaaaatatac aaagcatgta ataaccggcc
    12361 ttgccgtgat agtgttgata tcgtcattga ctccgttgaa tataatcgat gtgccggtgt
    12421 atgaacagac atacagactg gagaaggtac tcaagagaaa caatatgctt tccgacggtg
    12481 tagtgaaacc caataaggac atttcatatg ccgataagga gattatatcc agctgctacg
    12541 actatctcaa ctataaagat aaggcacccg aatatgtcag gtacaccaat tctttctacg
    12601 atattttcgg atttgaaaaa cttaacgagt acggcagttt ggatgaatac agcctgatac
    12661 atctaaacaa gagtaacagt tataacgcat tggatatatc gggatacagt cgctactata
    12721 ttgtcgatac gtcacgggaa agagaattga agatagttat tgacaatacg gagtacaata
    12781 ttgccgaaca tattatgaag ataaaagaag gtaatgacag atcggatatg gtatttgaca
    12841 ttacagaaga cataagggta tatttgacat atgtatacta tgagatgaat aatgaagggg
    12901 aagtgctgta cttcagtgca acgggctttg ctttgggtaa atagcaatat atttaacctt
    12961 gacatttgtt attttgttat ataataacat gagattagtg ccggataatt gacaggaggt
    13021 agacaatggg cagatacagg ttaataacga gatcggactt tgacggattg gtgtgtgcca
    13081 tgattttaaa ggaactggat atgttggagg acataatgtt tgtgcatccg aaggatatgc
    13141 aggacggtat gattgatgtt aacgaaaagg atatttccac caatgtaccg tatctgccgg
    13201 gtgtttatct ggcatttgat catcatatct cggagaaggg aaccaaggcc gttaactaca
    13261 taaataaacc tgatgctgcc tcaacggcaa gagtactgta tgaatatttc ggaggagcaa
    13321 gccgacttaa gatttccgaa gaactcatgg ccgccgtaga caaattcgat tcggcacaat
    13381 ataccatgga tgacatagtt aatccaaaag gttgggtaat gttgtcttat cttttagatg
    13441 caaggacggg cttgggcaga ttcagaaatt tcagaataac caactacgat cttatgatga
    13501 tgcttttgga ctattgcccc gaccacacta tcgatgaaat catgcgtctg cccgacgtac
    13561 aggaaagagt aaagatgtat tacgaacatg aaaaactttt ccgtgaccag ttaaaaggtt
    13621 gtgcggaagt ggttaaggat gtggtaatac ttcacttgaa gaactgcgat cccatatatg
    13681 taggcaacag attttatgta tatactctgt ttccgcaagc cacggtatcc attcatgaga
    13741 tgtggggact gaaaaagagg aataccgtct tcacactggg aaaatccatt ataaacagag
    13801 gttcggataa ggatttggct gctatatgta agaaacacgg cgggggtgga cttatgaatg
    13861 ccgcaacctg tcaggtaccc aacggtttgg cggacggaat actggaagac atactgcagc
    13921 agttattata aagcggagga ctttatggaa agatatgcat ggagagctaa agtaaaagaa
    13981 ggaatgctgg atgaatatat cagaaggcat gatgagattt ggccggaaat ggtaaagttg
    14041 cttaagaaag cgggaataaa gaattatacc atctggtaca acaataacga tttattcgga
    14101 tactatgagt gtgaacacgg tgtggaatat gccgcaaaag ttcaggcgga aagtcccata
    14161 gtggataaat ggaatgagta catgaaggat gtcatggtta tggagatgga tcccgttacc
    14221 aaagctcagc cgctgatgag aaaggtgttt ttcttagagt agttcaatat ataaaacatt
    14281 gatatatata aataaaaaaa gataagaaaa ataaaagctg ttctacagct ttatattttc
    14341 ttccaatgtt ctgttaagcc aaccgctttt tacaaaagga tcagagcatc gtgcaagttc
    14401 cttttttaac agctttctta aatcggagat tatatctgta taactgctgt catatatcag
    14461 gttttccagt tcgtaaggat cggtttgaag attataaaac tcatcaatat cggtaagatt
    14521 ccagatgtac ttatacttat cggtacgtat gcttctttgc gtgaaactgc cgaactgctg
    14581 tccgttggag gcggataccg tgtaatcggg aaattgttct ttattgggtt tttctccttt
    14641 aagaatactt aataaggatc ttccgtgaca atcgggattc cgctctattt tgcaaagttc
    14701 gcatatagta gccggaatat ccaatgcatt atatacaaag gtgtcacatc ttctataatc
    14761 aactgagtta ttcttaaaaa gcttagggta actcattatc ataggtattc tcagcacatc
    14821 atcatacatg atatagtgct tgtctatcat cccgtgagat ccgcacatat cgccatggtc
    14881 ggatgtatat atcaccaaag tgtcgtcata cataccgcat tcctttagtt tatccgatat
    14941 tattccgaag gaatgatcta tctgggtaat catggcataa tagcatctga cagtttccga
    15001 ccagtcttcc catgacatgt tttccagtct ccagttgagt atctgctgct tttggatgta
    15061 cggtttgttt ttgaaagtat caccgaatcc tccgaagggc ggtatgtctt ccggagcata
    15121 catgtcataa aactctttac aaggtctgca gggcaggtga gggtccactc cgtccaccca
    15181 tatgaaccag ggtttatcac cgtcttttaa tctgtccagc tgctcacata ccctgtcggc
    15241 ataatatttg ggtctgctgt cggaataagg gacgggattt acctcgccga accagctgtt
    15301 ggtgtattgc acattgggat atttttccga aatatactta ttgtattgct ttgacagatg
    15361 tgtcacattg aaaccgtatt gctcgggagg tattcttgaa acatcccatg cgcctatgaa
    15421 aatattctca tatcccgcat ctttaagact tttgggtatg ttccagtcgg tcggttgtac
    15481 ggtatttgtg ggtacaaacc cgaaattcca caatgcccct atggattcgg ctcgcattcc
    15541 gttcatgaac gattgtctgg cgggagcgca caccggtgtg ggacaatatg cattgtcaaa
    15601 tacaattcct tcttttgcta atctgtctat attgggagtg cggactgccc ggatattcga
    15661 gcagcctaca cagtcataac gatgctgatc tgccaaaaaa agcagaatat tgggtttttt
    15721 catcaaacta caaatcccgg tgttccgtgt aaaggaggaa gcgcacgggg acgcacaagt
    15781 gctccgcctt cttcaaagga ttttattgcg gcaacggtat attccaatgt cgcaagagca
    15841 tctcttcccg acccccttat atgctccgga ggaactttgt tggtcatatc ctctaagaat
    15901 gcatgcagtc tgttattgaa tgttataccg aaatcggtgg tgccgaaatc gaatacttcg
    15961 gcttgcattg tacccatgga tgcgggtttg gaaccgtccc agtaatacaa tttctccaca
    16021 cagttttcta tgcagaaagt tccttttgta ccggccattt caaaactcca ccatccgcct
    16081 aaaccgaata tggcgtctcc tctttgcgat aatatatatc ctatcgaacc gttctgatat
    16141 ttgacattta ttccgtttat cgaccacata aggtcgtttg cactcttgcg tccgcccggc
    16201 ttatcgaaaa aagcctgaac atgggttata tctccgccga aacttctcat caaagagaaa
    16261 ggatgagaga gaaatgcata tgcatgggaa tatgggcctg cgaatcttct catgggaccg
    16321 tctttgggaa gaggtttgta tgagtactcg cttccgttaa atcccacttt gtgaagtatg
    16381 tataccggag cacctatttt tccctgcgag ataagctctt ttgccttatc cgcgggaccg
    16441 gtaaacccgt gatttaagtt gcagcctaaa taaaggtttt tcttctgtgc agtaactacc
    16501 atttcacgtg cttcgtcatt gtcgttggat atgggttttt ccaccattac gttgaagccc
    16561 gcatccagag ccttcataac cggctcatag tgccaggaac cgttgtcaaa gcccgatgtg
    16621 gtaacatcaa tgagatccag ctcggggtga gctttcatca tgtcttcaat gcaatagaaa
    16681 gcctgcacat tatgctcatt agccgcttta tcggcttttt ccttaattac atcgcacact
    16741 gcgaccagtt gtgccagcgg gtcggcttta tagcatcttg catgcgtatt tcctattccg
    16801 cccattccta ctatccctat cttaattgcc attttttctc tccttattcc ttaatatttg
    16861 ctacaataac cggtttattt gtctttaaag actgtatagc cgcatgtatt atacggctgg
    16921 cttccaggcc gtctttgccc gatccgtcta tatcttcagg tttgacacct tccgcaactt
    16981 cccggacgaa tgtatgtatc ctgtcttcaa atgtgtcgta gaagtctcgg aatccgccga
    17041 atacgggatt ggtatatact cttttctcat aatttcccgc agggtagagt gtggcctctc
    17101 tccacatgtc ttcaaatatc agtctgcctt ttgttcccgc gacctcgcat ctttccatgg
    17161 gatgacctct ttctatgtca taggaactgg tgaggtgacc taccgcaccg tttacgaact
    17221 tcatatttat agatgccgta gaccatattt ctcttccggg ggctttcatg gcatagcagt
    17281 gaacctgttc cacatcgcct ccgaaatatc tcaatatatc gcaggaatgc ggattaagag
    17341 ccttaagatg atagtattcc gtgtcaaaat gcggttggaa tctgcctatc cacaatgcca
    17401 tgttcaaaaa gagcaggtta cctattaaac cttcattctg ccatttcttt gcgatacggg
    17461 cagcaggtgt aaatctgtga tttaagtcta taccgaagca tctgtctaat tttttagcca
    17521 aatccaccat ttcctgaccg tgttgcaaat tgttggatat gggtttttcc gtcagtacat
    17581 ggcagcccgc gttgagtgcc tgcatggtgg ggatgtagtg atcggaactg tattcaaatc
    17641 cgcctgtagc cacgcttaca atgtccggtt tctccgcttt taacatctct tttgcatcat
    17701 aatatgccgg aaccacatat ctttcctttg ccgcatcggc tctgtctttc cgaatatcgc
    17761 ataccgctac aagttccgcc tgcttgcagc ttttataggc ttttgcatga ttgttgccga
    17821 taggtcccat gcctatgacg gctgttctta acatgatatg cctccttatt tctgctgcaa
    17881 tttggctgcc gttttggctg aggctatgtg catggagttg taagcttctt ccgaggatat
    17941 gaagcctctg ttaagatcca gtccgtgcca tgtaaggttg gtatatatgg gattggttct
    18001 tccgacttct ttttcgcgct tccttataaa ttcactcatt tttttatcca gcatcataac
    18061 tatatccggc cggtcctttg caatattcca cagttccatg ggatcatttt ccagatcata
    18121 cagttccact tccggtttga aatggaaatc cggctccaat gcctgaatat atttccactt
    18181 gggagttctc caaccgtgtt ttctcatcca tgtacattcg gtgatgtaaa attcgtcaag
    18241 cttcggtatc ggttgtccga atgccaaagg aaatacactg cgtccgtcaa agtttatacg
    18301 cgtttctatt ccgagaatat ccagaaccgt aggcgtaaca tcatacagtc tgcaggtatc
    18361 gtccactctc acaccttcgg gtactctgcc cgggaaacgg aaagcaagag gcaccactaa
    18421 agtgggatca tataaactgt gatgatcaaa atagcagtcg tgatcataaa gtgtttctcc
    18481 gtggtcggat gtgaatacta caagtgtttc attctccagt cccaggtcgt aaagtttctg
    18541 cagtatcgac tgaatgcagg tatccatgta tgcaatagcc gcatcatact gggcaataac
    18601 ataatcttta tcggtacatc cttcgggaat ccattccgcg aaatagtcac aaaacggttt
    18661 gaatgccata acaggttcca tcgagcggtt gttggggtca aattcattac cctgataaaa
    18721 cattctttcc cagggtccgg ggggaagata aggtgaatgg gggtccatgt gccgtaagaa
    18781 caggaagaaa ggcttatcct gtgctgccag tctttccagt tccggtatag ccactgcgtt
    18841 catactctcg gctttatggg cccttttatc atgttcctca atactccagc cgctgtattg
    18901 tatgtaattg tcaaatcctc ttccggaaac attcttaaat cctatgtttg tggtggtata
    18961 cccgtgtgac gaaagaatct ctgccagtgt tttaacaccg ggagcgatat ctcctttatg
    19021 tctcaaagct actatgtcgg taccgaaaca gtcgaaaccg gtaagcatat tggcatatcc
    19081 cggagttgtc gggatatgcg gtgagaaaca gttattgtac accgttcctt ttttcaggta
    19141 cttgtcgata ttgggagttg tcagcctgtt gtagccgtag aggctcatat ggtctcttct
    19201 taagctgtca attccgaaga ataatagatt gggtttcttt gccatgttat ctctctcctt
    19261 ttaacttata taactgatat actttaattt tccgttcatt ttattgataa attaccgtta
    19321 ataacttacc ttgcattaag tatcttaagg caggcattta tgtatccgta actttccgcc
    19381 gctatacgcg tggcttccac catatcctgt tccggcccta taacttccag attgaccgga
    19441 ccgctgtaac cggcctccac catcttggat atgtaaccga acaggtagat atcgccttta
    19501 ccgcaagcct gcaaagacgg gtgtccggga cccggtcctc ttcctttgca gtcacgtata
    19561 tgtatatgtt tcattgccgg taataccgcc gctattgctt cttctggcaa ttccgatgcc
    19621 ctgtatatat gggaagggtc catatccact ccgaaattgg ggctgtccac catttgcata
    19681 acccttaatg tggtttcggt gttgtgcata gatgctccca catgagcttt acagcatatt
    19741 gtcaccccgt atttctccgc catttttgca agttccgaaa gacttcttgc ggtctcgata
    19801 agatcttctt cattgccggc ttttcctccc ggaccgacat tcagtacggg gatgccgaag
    19861 tattccgcag ccgcaagcgc ttttttcatt ctctttttgt ctctggatgc cacttcagag
    19921 gctaaaattg taaggccggt ggcctccagt attgccttaa tttctctttt ctgtgctctc
    19981 cagttttcca cctgaagatg ctcgcacatt cccggtatgg cggaaatttc cacgccgtca
    20041 taaccgcatt ttgctatggc ttcggcagcc tgaccgaatg tgtaatgctt gaaaagtact
    20101 gagtttactc ctaatttgat catacggttt ttcctttcga ttaataaata ctcacaccta
    20161 tgaattatat atcagcaaag gggaaaatca acatcaaaaa cccttattta acccttatta
    20221 tacattttac actaagaatt taatgtttat tattaagtgt ttaaaaatat attacgtata
    20281 tcggttaact ttattgagtg taaataaggt ataatggagt gcgtgctgac aggaggcgga
    20341 atatcttgaa aaaacctgaa ttgtttttac tgggagtgga cggaggaatg acttcgtata
    20401 taacggagca gatagag
//
LOCUS       DAOSOM010000034        19319 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_2835_length_19319_cov_5.771231, whole genome shotgun sequence.
ACCESSION   DAOSOM010000034 DAOSOM010000000
VERSION     DAOSOM010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 19319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19319
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2835_length_19319_cov_5.771231"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1527
                     /locus_tag="PLF14_07750"
     CDS             <1..1527
                     /locus_tag="PLF14_07750"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOT71217.1"
                     /translation="LKMRPDYISGYVHIASDVSLDTVAYITENSHKLGGVSLRKSYVR
                     AYTEDAAKVAHIIGYVGLVSEQELKADETLSPDYMIGKMGVEQYCEKYLRGQDGYAVV
                     ETDVSGKVISVVEGKRPINGNDVYLTIDIELQKAALNALEVTIEEIIAKADNEKNFGD
                     ANAAVAIVIDVDTGELLVCANNKYFDPSWFVDNDEQSVGKRMETITDEVNTPMFNRAI
                     QAMYTPGSIFKPIVAIAGLESNTITAESLIECRMKAEIGEHNFTCLSWHGKINVTKAI
                     EQSCNIFFNQLGVDTGIDTIDAWARAFGFGEKTGIDLPNESSGIISGKEYKMQTFGER
                     WWPADTAQTAIGQLYNNFTPIQIASYTATLASAGKKYTPHVVLKIVDESGDIVYEGDK
                     SYTDIGMSKETDATIRHAMEQVAKEGTASRIFSKYPLRVAAKTGTAETGWEVDSSSNG
                     LSIAYAPADNPEIAACVVIEKGVWGSYTVPALMRIFNEYFDIQPEEEIIDDTLVTNEQ
                     "
     gene            1570..2100
                     /locus_tag="PLF14_07755"
     CDS             1570..2100
                     /locus_tag="PLF14_07755"
                     /inference="COORDINATES: protein motif:HMM:NF014614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOT71218.1"
                     /translation="MKGLIHVYTGEGKGKTTACTGAAIRAAGSGMKVYFVQFQKGFDS
                     SELNILRKTENIEVNRICTFTKFYFYMKEQEKALYTKEHREAFNQLGENIKKQPLKYD
                     MLVLDEILGAVKLGIIAEDSLSDFLKTKPAHLEVLLSGRDASENIINLADYVSDIKCV
                     KHPYNSNIASRKGIEY"
     gene            2136..3011
                     /locus_tag="PLF14_07760"
     CDS             2136..3011
                     /locus_tag="PLF14_07760"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT71219.1"
                     /translation="MICISDKWKDYELLETGDKEKTERWGEYILRRPDPQCIWPYPAD
                     SNIKPHGVYNRSSTGGGSWQWKKSVPERWQISYGPLKFHVEPTGFKHTGLFPEQAYNW
                     DFLTDKIKDEYKAREGKINVLNLFAYTGGATVACAYAGASVCHVDAAKGMVARAKENA
                     RLSGLADAKIRYIVDDAVKFIKREINRNNKYDIVIMDPPSYGRGPKGEMWQVEDSLYN
                     LIYLCMQVLSDTPSVFMVNSYASDISATAIENILELTVNSKYPGIITSYELGLKQSNS
                     SVILPCGYTIRWESK"
     gene            2996..3667
                     /locus_tag="PLF14_07765"
     CDS             2996..3667
                     /locus_tag="PLF14_07765"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOT71220.1"
                     /translation="MGEQVNILYEDNHIIVVVKAADQPVMQDASDDYDLLTEVKDYIK
                     IKYNKPGNVYAGLVHRLDRPVGGVMVFAKTSKAASRLSEQIRNNVITRKYMAVVSGRM
                     SKTSDTLENLICKDKDKRMSFVSDTTCKEAKLARLSYTVLKTVGDYSLLDIELDTGRH
                     HQIRVQLANIGHPIYGDMKYGKGPKGNIALFSYSLELIHPVTKEIMKFTAEPDYTKAP
                     WPLFK"
     gene            complement(3634..4500)
                     /locus_tag="PLF14_07770"
     CDS             complement(3634..4500)
                     /locus_tag="PLF14_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT71221.1"
                     /translation="MNTDSEIEKKINRKKLLINILLFAAFPIICLVFALIGKWLIFGI
                     VLLIYLAVFVYIKLENIYLIQTGYFDKKGELQKTLKPAYKAYKIKNSTIDTANIFIYM
                     LLKAGKYDKALEITEDNRNRYMTEDQYIAFSSNEALALWKTGKTEQSVALFDKIASKF
                     ESTSLYVSYGTVLTYSDDLNKALQVNKKAYEYNSGSKGIKDNLAYTYFLIGDTDRARR
                     MYDELLSEPLNFPEAYYNAALVYCSQGDYDEGVRLMKSALTKQFNGLTAVTKEEISEK
                     IDYLNRGQGALV"
     gene            4689..5336
                     /locus_tag="PLF14_07775"
     CDS             4689..5336
                     /locus_tag="PLF14_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOT71222.1"
                     /translation="MRLCKNCGTNNSFGSRYCEECGQKLEIKLEFPKIKKPVQINKNA
                     IITLLILAVIITLLVLLISSSGRIEQSLDLYFSGLQKNQMERFIDAFPPQVEKIFTHG
                     SKIDNIKHEDFRMTFDEDYADNLARFGNRFRVRYTVKVKEYWSKEEINSRKIQLYDIW
                     GIPKTSVKDIITVNLRVTLRGDRQEQSYTMALDMVKIRGRWYVYPDYDAFMHTKP"
     gene            5524..6357
                     /locus_tag="PLF14_07780"
     CDS             5524..6357
                     /locus_tag="PLF14_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71223.1"
                     /translation="MGLLSLKCPSCGANLNVKEGRDDYFCQYCGAKLEYAKQRIELSG
                     TVNVSGMANEATLLERAFLYAEDSNFNDAKVYFNKVLDINPRNAKAYMGLLICKLGIR
                     TIDDLQNYDYPISQHEEYQKAVRFAGLKEKQQYDSLNTSLLERYISMMSSRRAEIEEL
                     NNKIISDKKIGNDNRINISRLKAKETILLTILIIIIILDLLSVTGIILEPPFAIIFLV
                     ILGIPAFFVIRALIRTKKRIKEAANDNINLFRCIMDNEVKIKTLKKEYNDASIGIDLQ
                     G"
     gene            6443..6928
                     /locus_tag="PLF14_07785"
     CDS             6443..6928
                     /locus_tag="PLF14_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71224.1"
                     /translation="MDIERIKIEYMPKITDDQLYDFYVRNDICEAGHGKEAAVKPLKY
                     SPYMVGAFYEGKLVGIVRAVFDGLTAVIMEFCLELELQGDNLQYDNGSIIEKDRYGIA
                     KEMGILLIDELRKIGNTFVSAVILQHSEKALYSSIGFKCNEGHAEYIIDERPYVLSEI
                     E"
     gene            7054..7866
                     /locus_tag="PLF14_07790"
     CDS             7054..7866
                     /locus_tag="PLF14_07790"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOT71225.1"
                     /translation="MKCVKCGNELEANTKFCASCGTAVEQAKPAAKETAEQKKPAAKP
                     ATKAADKPAEKPAAKPAEKPAAKKAAEKPAEKPAEKPAGSAGFCNNCGAALEPGAKFC
                     AGCGAGVGAPGAAAGPTPFAAMPPVQPQKGAKETFEDINRTPDTTDEYDPEEIEMFKR
                     ITVVAYLGYLVLIPWICFPKIKYVRFHSNQGLILAILNTIFGAITSILSALSRALLVN
                     VRWLGIIFSILTAIISLAGGIVIIYLIVVGIINAVKGRAKELPLIGKIRLLK"
     gene            8032..8157
                     /locus_tag="PLF14_07795"
     CDS             8032..8157
                     /locus_tag="PLF14_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71226.1"
                     /translation="MADLSVNIIINIVSALSVIIAVIALYAVGESYKQSQGEQTV"
     gene            8229..9890
                     /gene="ilvB"
                     /locus_tag="PLF14_07800"
     CDS             8229..9890
                     /gene="ilvB"
                     /locus_tag="PLF14_07800"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019892110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOT71227.1"
                     /translation="MKATGTKVIIETLIEQGCDTVFGYPGGAVLSIYDELYGHSDRIR
                     HITCAHEQGAAHAADAYSRVTGKVGVVIATSGPGATNLVTGIANAYLDSSPVVAITGN
                     VPLSLLGKDSFQEVDITGITMPVTKHNYIVKDVNELANLIREAFFIAQSGRPGPVLID
                     VPKDVQTAVCDFEHKKVIKKKRVCSATEEQLLKATELISQSKKPYIYCGGGAVISQAG
                     KEIAKLAERIDSPIGFSMMGLSAVSADNPYWLGMTGMHGRFASTKAHSEADLIIAAGV
                     RFSDRATGNRSKYTQNAKVIHIDIDPAEINKNIETHCSLVGDVGTVVTKLLDKVEKKT
                     NTQWMDAVQKMKDLKEMRSCKDSVMHPEDIIEKVMEYTDASTIVATDVGQHQMWVAQY
                     YRFKKPRTHLTSGGLGAMGFGMGAAIGGCVASGGKRTVLFTGDGSFGMNLIELATAVT
                     YKLPLVILIFNNGVLGMVRQWQHAFFDSRYSHTTLDRKTDFVKLAQAFGASGYRATDI
                     KTLDEALKKAFSTQGPAVIDCVIDKEEKVYPMIPPEGSIDDIITR"
     gene            9904..10413
                     /gene="ilvN"
                     /locus_tag="PLF14_07805"
     CDS             9904..10413
                     /gene="ilvN"
                     /locus_tag="PLF14_07805"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOT71228.1"
                     /translation="MKQGQFILSVLVANHAGVLTRVSGLFSRRNYNIDSLNVAVTEDP
                     LFSRMTIIARGDQAVREQITKQLSKLQDVKKIELLTPEKTVLREHLLIKIKVTKKTRG
                     EIVESVNIFRGRVVDFNIDALTVEVTGESDKLDAFIKFVSQFGILEMCRAGALAVKRG
                     AGNDNKEEE"
     gene            10424..11992
                     /gene="cimA"
                     /locus_tag="PLF14_07810"
     CDS             10424..11992
                     /gene="cimA"
                     /locus_tag="PLF14_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005211113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HOT71229.1"
                     /translation="MVGRRIEILDSTLRDGAQSEGISFSVSDKLNIVKALDCFGIDYI
                     EAGNPGSNPKDMDFFQQAARLQLKHAKLCAFGSTCRTGIHPKDDKNIRALINTGLDTL
                     VIFGKAWDLHVKKVLNISPEENLKIVEQTISYLKSLGKYIIFDAEHFFDGYKSDADYA
                     LKVLEYAVKGGADVLTLCDTNGGSLPDEAEKITKAVVSLFPHCKIAIHCHNDTGCAVA
                     NSLIAVNAGAGMIHGTFTGIGERCGNADLSIILPNLKLKMGYECNGDISQLYSVARRI
                     AYISNTQIDNNRPYVGKSAFAHKGGMHADGVLKVHSSFEHVDPESVGNKRKFLMSEVA
                     GRSTLITKASAIVPNLTKDSKEVSKILEKLKEMEHYGYQFEAADASFELLVKRTVGLY
                     KPHFHLVLYKTMSEFPVPTGGMPASATIKVEVDGKEEITAVMGNGPVNALDLALRKSL
                     TVFFPAIKEIQLMDYKVRVLEENSTTAAKVRVLIETSDGKKSWSTIGVSNDIIEASLI
                     ALVDSIEYKLSMNE"
     gene            complement(11995..12432)
                     /locus_tag="PLF14_07815"
     CDS             complement(11995..12432)
                     /locus_tag="PLF14_07815"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOT71230.1"
                     /translation="MNCSKCGTLLKENAKFCSGCGAQVPKVQSASVSCAYCGAALVPG
                     AKFCAGCGKPATPKQSNTCPRCNNPLVAGAKFCAYCGASTAAASNVCPRCNTPFKPGA
                     KFCGVCGAPATSATLPPLNVCKNCGTPFKPGTRFCGKCGSAIL"
     gene            complement(12566..13486)
                     /locus_tag="PLF14_07820"
     CDS             complement(12566..13486)
                     /locus_tag="PLF14_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71231.1"
                     /translation="MPLVAAKCTQCGANLQIDSTKDAAICPNCNTAFVTEKAIINYKT
                     YYEYKIEKADVHIHDEKSIEIRLKNAEIFFKKHNKVDKAHELFHSVANDAPGDYRGWW
                     GLVRVKTDDFGTLEISRTDVEDIKHFVNCTFNVAPADILDKLEQTWRTYNQGVYKFHS
                     QLSLDKEQWAHQLKITEAETFNLQNTISMLAVKIKQSDLRYNNHARKCGSTTLPFIIT
                     LTAVSVLLLMAGILGKVGVLTGISIAGFVISAISFVSYFIHKHLMKKEARIKQEMEQQ
                     RKKTINTVTELFEKKDKLKRQICYAEEMLS"
     gene            complement(13655..15001)
                     /locus_tag="PLF14_07825"
     CDS             complement(13655..15001)
                     /locus_tag="PLF14_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013141331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HOT71232.1"
                     /translation="MFKLKENGTKVSTEILAGLTTFVTMAYIIAVNPAMLSLAGMEWG
                     AVFLATIIASIIGTLIMGLVANVPYAQAPGMGLNALFVFTVCFTYGFSWQHALSMVFI
                     CGVINILITVTKIRKHIIKAIPKSLQNAIGGGIGIFIAYIGLLNVGAINFGAGVPSMS
                     VINTGAIYVFIIGLVLTIVLLLLKVKGAILIGIVAATLIGIPLGVTTSADKVSFIDAV
                     NVLPTTLGVIFTKEGLGSLFSDVSKLPIVLITIFAMSLSDTFDTIGTFIGTGRQTGIF
                     SEEDEKAMESSSGFKSKMDKALFADATATSIGAIFGTSNTTTYVESAAGIAVGGRTGL
                     TSVVVAICFALSAFFASFISSIPGAATAPALVVVGIMMLSSFKEIQWSELEEAIPAFF
                     AGIFMALAYSITTGIAMGFIFYLIVKVCKRQFKDISPVLWVASALFILNFVLQAII"
     gene            complement(15272..15679)
                     /locus_tag="PLF14_07830"
     CDS             complement(15272..15679)
                     /locus_tag="PLF14_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004268913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4234 domain-containing protein"
                     /protein_id="HOT71233.1"
                     /translation="MQVTPLQTNRSLGRFILLSIVTLGIYGIVYFYKVGRDLNTVCSP
                     YDGKKTMHFVLLALLIAPLTAGIGGIVWIHKMSNRVGWALTHRNIAYTFNSNTYWLWG
                     VLGSLIIVGPFIYLHRLSIAMNYICQDFNARGQ"
     gene            complement(15777..16982)
                     /locus_tag="PLF14_07835"
     CDS             complement(15777..16982)
                     /locus_tag="PLF14_07835"
                     /inference="COORDINATES: protein motif:HMM:NF013377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HOT71234.1"
                     /translation="MEMQMPSKQLIREKKESVLDRIYNYHLKKMAGSKGNVFLISDTY
                     PGVWLEHVYDAVSWVNYDKTDPTVSVHQIRLFLDRQKPDGQFPCYVIDPEKGNKFKNK
                     IGYSQIQECLSFARLCLETCIQNNNMELLAECYDRCKKWDNWLTQNRMTMKTGLIEMF
                     CGFDTGHDNSGRFANIKYKGNISEDAKDYPHDDHSLPHITPDMNAVFYGSKTALAVMA
                     GKLGLEQEAHDWKQAAREVKKKILEICYDEQDDFFYDVDRHGNKIKCKSISITNLFCE
                     NVLDVDMAKSIFSKYLDNTEEFKTPYPYPAVSISDPLWIQNRQGNSWGFYSQGLVALR
                     SLRWMDKYGFSEQLEDNMKKWITAWCLTDKLNFGQELHPITGEPSSCGNWYSSTMLYF
                     IHSLRRLGL"
     gene            17133..18119
                     /locus_tag="PLF14_07840"
     CDS             17133..18119
                     /locus_tag="PLF14_07840"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOT71235.1"
                     /translation="MISKELYHKIHMEFQVLIHPDELSYEWIDRAIANDIDAIGIHPV
                     GGYRARESLADLVERFKDNDFTDLIDYASDNGIKIEYEMHAASYLMPRELFSTHPEYF
                     RVDETGHRNNDANFCVSNQEALQYVSNAAAILASGLYKSSDRYYFWLDDVKNKKCMCE
                     KCRNLSMSDQQMIFINAALKEIQKSNRKAKMAYLAYFDCITPPQKIKPVKGVFAEYAP
                     MGKYVSETQTRENSKINEESDINNLFSVFSKSNSKVLEYWLDNSMFSNWTKPEKKFVP
                     DNKMIKEDIEKYHSLGFEYISSFACYLGPQYTKLHGQPDLSAFFRCVEKYKG"
     gene            complement(18197..18281)
                     /locus_tag="PLF14_07845"
     tRNA            complement(18197..18281)
                     /locus_tag="PLF14_07845"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18245..18247),aa:Tyr,seq:gta)
     gene            complement(18298..18374)
                     /locus_tag="PLF14_07850"
     tRNA            complement(18298..18374)
                     /locus_tag="PLF14_07850"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18338..18340),aa:Asp,seq:gtc)
     gene            complement(18377..18451)
                     /locus_tag="PLF14_07855"
     tRNA            complement(18377..18451)
                     /locus_tag="PLF14_07855"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18416..18418),aa:Glu,seq:ttc)
     gene            complement(18523..19173)
                     /locus_tag="PLF14_07860"
     CDS             complement(18523..19173)
                     /locus_tag="PLF14_07860"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HOT71236.1"
                     /translation="MVIKLSKDDYTIDKEDIIKRLRISEDDIDEFNEVYDKCISIACP
                     KYLFTKKEITGIDDDWVRIEDVIINSRIAAKNLRHTGYVYPYVATCGHEVHEYSKSIS
                     DPFVGYWIDYIMEKLLHTAIRCLNRDMKNTYDLKKTASVNPGSTVDWPISGQAQLFEI
                     MKEEIKDTGVTLTPSFLMLPHKSVSGLTFITDKDYVNCQYCQRKNCPNRRKEYTPE"
BASE COUNT         6019 a         3611 c         4261 g         5428 t
ORIGIN      
        1 ctcaaaatga gaccggacta tatatcgggt tatgttcata tagcatcgga tgtatcgctg
       61 gataccgtgg catatataac ggagaacagc cataaactcg gaggggtatc attacggaaa
      121 agttatgtaa gagcctatac cgaagatgcg gcaaaagtgg cgcatattat aggttatgtg
      181 gggctggtgt cggaacagga acttaaagcc gatgaaacgc tttcgcccga ctatatgata
      241 ggcaagatgg gagtggagca gtattgtgag aaatatcttc gcggtcagga cggatatgcc
      301 gttgtggaaa ccgatgtttc gggaaaggtg atatcggtag tggaaggcaa aaggccgata
      361 aacggcaacg atgtatatct cacaattgat attgagctgc aaaaagctgc tctgaatgct
      421 ctggaagtca ctattgaaga aattatagca aaagccgata atgaaaagaa cttcggagat
      481 gccaatgccg cagtcgccat agttatagat gtggataccg gtgaattatt ggtctgtgcc
      541 aataataaat attttgatcc ctcatggttt gtcgataatg atgagcaatc ggttggaaaa
      601 aggatggaaa ccataaccga cgaggtaaat acgcctatgt tcaacagagc cattcaggca
      661 atgtataccc ccggttccat tttcaaaccc atagttgcca ttgccggtct ggagagcaat
      721 acaataaccg ccgaatcgct gattgaatgc agaatgaagg cggaaatagg ggaacacaat
      781 tttacgtgtt taagttggca cggaaaaata aatgtaacca aagctataga acaatcgtgt
      841 aacatatttt ttaatcaact gggtgtggat acaggaatag ataccataga tgcatgggca
      901 agagcattcg gattcggaga aaagacgggt attgaccttc ccaatgaaag cagcggaatt
      961 atcagcggta aagagtataa gatgcagaca ttcggggaaa ggtggtggcc ggccgatacc
     1021 gcacaaacgg caataggaca gttgtataac aacttcaccc cgattcagat agcaagttat
     1081 acggccactt tagcttcggc aggtaagaaa tataccccgc atgtagtatt aaagattgtg
     1141 gatgagtccg gtgatattgt atacgaaggg gataaatcct ataccgatat agggatgtca
     1201 aaagaaaccg acgctactat acgtcatgcc atggagcagg ttgctaaaga aggtaccgca
     1261 agcagaatat tttctaaata tcctttaagg gttgcagcca aaacaggaac ggcggaaacg
     1321 ggatgggaag tggattcttc ttccaacggt ctttccatag cctacgcacc tgcagataac
     1381 cccgagattg ccgcttgtgt tgtaatagaa aaaggcgtgt ggggttccta caccgttccg
     1441 gctttaatga gaatattcaa tgaatatttc gatattcaac cggaagaaga gataatagat
     1501 gatactttgg ttacaaatga acagtaaagg aggaaaatcc ggtcaccgcg ggtgcttgga
     1561 ttatacgtta tgaaaggtct cattcatgtt tatacgggag agggtaaagg caaaaccacg
     1621 gcatgtacag gtgccgcaat aagagcggcc gggtccggga tgaaagtata ttttgtacaa
     1681 ttccagaagg gatttgacag cagcgaactg aatatactga gaaagaccga gaatattgaa
     1741 gtaaacagga tatgcacttt taccaagttc tatttctata tgaaagagca ggaaaaagca
     1801 ctttatacaa aagaacatag agaggctttc aatcaactcg gagagaatat taaaaagcaa
     1861 cctctgaagt atgacatgct ggttctggat gaaatcttag gtgcggttaa attgggtatt
     1921 atagcggaag attcactttc ggattttctt aaaacaaagc ctgcgcatct ggaggttctg
     1981 ttaagcggaa gagatgcatc ggaaaatatc attaatcttg cggattatgt gtcggatata
     2041 aagtgtgtga aacatcctta caacagtaat attgcctcca gaaaaggtat agaatattaa
     2101 taaggtaata tggaatacat ggcagagggt tataaatgat ttgcatatcg gataaatgga
     2161 aagattacga gttattggaa accggtgaca aagaaaagac cgaaagatgg ggagaatata
     2221 tcttgcgaag acccgacccg cagtgtatct ggccttatcc cgcagacagt aatataaaac
     2281 cccacggggt atacaatcgc tcctctaccg gcggaggaag ctggcagtgg aagaaatctg
     2341 ttccggaaag atggcagata agttacggac cgcttaaatt tcatgtggag cctaccggtt
     2401 tcaagcatac cggattgttt cccgaacagg catataactg ggattttctt accgataaaa
     2461 taaaggatga gtataaggca agagagggta aaatcaatgt cctgaatctt tttgcatata
     2521 caggcggtgc cacggtggcc tgtgcatatg caggcgcttc ggtgtgtcat gtggatgccg
     2581 ccaaaggtat ggttgcgcgg gcaaaggaaa atgcccggtt atcgggattg gcggatgcca
     2641 agataagata catagtggac gatgccgtaa agttcataaa aagggaaatt aacaggaata
     2701 ataaatacga tatagtgata atggatccgc cttcctacgg aagagggccc aaaggggaga
     2761 tgtggcaggt ggaagacagc ttatataacc tcatatattt atgtatgcag gtgttaagcg
     2821 atacgccttc ggttttcatg gttaattcct acgcatcgga tatatcggca accgccatag
     2881 aaaacatact ggaactgact gtaaacagta aatatcccgg cattattaca tcttatgaat
     2941 tgggattgaa acagtcgaac agttcggtta ttctgccttg cggatataca ataagatggg
     3001 agagcaagtg aatatattat acgaggacaa tcatataata gtcgtggtaa aagcagccga
     3061 tcagcccgtt atgcaggatg cttccgatga ttatgatctt cttaccgagg ttaaggacta
     3121 tattaagatt aaatacaata aaccgggaaa tgtttatgcg ggacttgtgc acaggttgga
     3181 cagaccggta ggcggggtaa tggtttttgc caagacatcc aaagcggctt ccagattaag
     3241 tgaacagata agaaacaatg tgattacaag aaaatatatg gccgtagtat cgggaagaat
     3301 gagtaagaca agcgatacat tggaaaacct tatctgtaaa gataaggata agaggatgtc
     3361 gtttgtttcg gacacaacat gtaaagaagc caaacttgcc agactcagtt ataccgtatt
     3421 aaagacggtt ggcgattatt cattgctgga tattgagttg gataccggca gacatcatca
     3481 gataagagta cagcttgcaa atatagggca tccgatttac ggagacatga agtacggcaa
     3541 aggacccaaa ggcaatatcg ctttgttttc ctacagtctg gaacttattc atcccgttac
     3601 aaaagagata atgaaattta ctgccgagcc ggactacacc aaagcgccct ggcctctgtt
     3661 taaatagtcg attttttccg atatttcttc ttttgtcaca gcggtcaacc cgttgaattg
     3721 ttttgtcaac gcactcttca tcagtcttac gccttcgtca taatcgcctt gtgaacagta
     3781 aaccaatgcg gcattgtagt atgcctcggg aaaattcagc ggctcgctca ataattcgtc
     3841 atacattctt cttgcccggt cggtatcgcc tataaggaaa tatgtgtatg ccagattatc
     3901 ttttatacct ttgctcccgg agttatattc atatgccttt ttattgacct gtagcgcctt
     3961 attcaagtca tcggaatatg tgagcacggt accgtaagac acatacaggc tggtggattc
     4021 gaattttgaa gctatcttat caaagagtgc taccgattgt tctgtttttc cggttttcca
     4081 caaagctaaa gcttcattgg aggaaaaagc gatatactga tcttcggtca tgtaacgatt
     4141 gcggttgtct tcggttatct ccaaagcttt atcgtattta cccgctttca acaacatata
     4201 tatgaatata ttggcggtgt ctattgtgga gtttttaatc ttatatgctt tataggcggg
     4261 ttttaaggtt ttttgcagtt cacccttctt atcgaaatat cccgtctgta tcagatagat
     4321 attttccaac tttatataca caaagaccgc aagatatatt aaaagaacta tcccgaatat
     4381 gagccatttt cctatcaaag caaatacaag acatattatg gggaatgcgg caaacagtaa
     4441 tatatttatt aaaagtttct ttctgtttat ttttttctcg atttcgctgt cggtattcat
     4501 tgctatcctt tttcataaac aatcttacta tattataccg taaaaatcat tgtttatctc
     4561 atattaatat tttccatcaa actatacaca aatcaacctg aagggttgga atatataaat
     4621 accttaagtg gcaaatattg atattagtta acgggacata tagaatatag atatacggag
     4681 gagaaacggt gcgtttatgt aaaaactgcg gaaccaacaa cagtttcggt tccaggtatt
     4741 gtgaagagtg cggacaaaag ctggagatta agctggagtt tccgaaaata aagaaacccg
     4801 tacagattaa taaaaatgca ataatcactt tattgatttt ggctgtcata atcactctgc
     4861 ttgtactgct tatctcttcg tccggcagaa tcgaacagtc tctggattta tacttcagcg
     4921 gtctgcaaaa gaatcagatg gagcgattca ttgatgcttt tccgcctcag gtggagaaaa
     4981 tatttacaca cggaagcaag atagataata taaagcatga agatttccgg atgacattcg
     5041 atgaggatta tgcggataat cttgcccgat tcggtaaccg gttccgggtc agatataccg
     5101 ttaaggtaaa ggaatattgg tctaaagaag aaatcaattc acggaaaatc caattatatg
     5161 atatctgggg tatccccaaa acatcggtta aagatataat taccgttaat cttcgggtaa
     5221 ctctcagagg tgacagacag gagcaatcat atacaatggc tctggatatg gttaaaatac
     5281 gcggcagatg gtatgtctat cccgattatg atgcttttat gcataccaaa ccgtaatttt
     5341 ccgagtaatg ttttttacaa cgaagctaaa gtagaaaagg aaaaccagaa gaatcttcac
     5401 tttaaaaata gagaaacgtg tgctatactg taaaaagttg atgattaaaa taattagtaa
     5461 agcaaggata acttaggatt tttcaaaact gtcggaaatt gttaaacgaa agggtatggt
     5521 tctatggggt tactttcact taaatgtcca agctgtggtg ctaatttaaa tgtaaaagaa
     5581 ggcagagatg attatttctg tcaatattgc ggtgcgaaac ttgaatatgc gaaacaaaga
     5641 attgagttgt ccggaaccgt aaatgtatca ggaatggcga acgaagcaac attattggaa
     5701 agagctttct tatatgctga agatagtaat tttaatgatg caaaagttta tttcaataag
     5761 gttttggata taaatcctcg gaacgcaaaa gcatacatgg gacttttgat ttgtaaactc
     5821 ggaatcagga caattgatga cctgcaaaat tatgattatc ctatatctca acatgaagaa
     5881 tatcagaaag cggttcgctt tgccggttta aaagaaaagc aacaatatga ttcgttaaat
     5941 acatctctct tggaaaggta tatatcaatg atgtcttcca gaagggcgga aatagaagag
     6001 ttgaataata agataattag tgataaaaaa atcggaaacg ataatagaat aaacatttca
     6061 agattaaagg caaaagaaac catactactc acgatattaa ttatcattat tattcttgat
     6121 ttgctttctg ttacaggaat aattttggaa cctcctttcg caataatatt cttagttata
     6181 ttaggaattc ctgcattttt tgtcattaga gcattgatta gaacaaagaa aagaattaaa
     6241 gaagctgcta atgataatat taatcttttc agatgtataa tggataatga agtaaagatt
     6301 aaaactttga aaaaagagta taatgacgca agtataggca ttgacttaca gggataaaga
     6361 aataaaacag aatcgtagaa tttaggagcg tgttttaacc atgtattcta cagtaaatat
     6421 aataacatgg agtgacaacc acatggatat tgaaagaatt aaaatagagt atatgcctaa
     6481 aattaccgac gaccagctgt atgatttcta tgtgagaaac gatatttgtg aagccggaca
     6541 cggtaaggaa gctgcggtga aacctctgaa atatagtccg tatatggtag gtgctttcta
     6601 cgaggggaaa ctggtgggaa tagtaagagc ggtattcgac ggtttgacag ccgttataat
     6661 ggagttctgt ttggagttgg aattacaagg cgacaatttg caatatgata acggttccat
     6721 aatagaaaaa gaccgatacg gtatagcaaa ggaaatgggc atcttactga tagacgagtt
     6781 gaggaaaata gggaatactt ttgtttccgc agtaattttg caacattccg aaaaagcctt
     6841 atacagttcc atcggtttta aatgtaatga aggacatgcg gaatatatta tagatgagcg
     6901 tccgtatgtg ttatcggaaa ttgaatagca aatctgttga aatataacgt tttaattcgg
     6961 gacacctttt taatacggga tattgcacag aaacatattt ttgtgttatt ataaataagt
     7021 acttaataaa tatatagaga ggggtagcaa attatgaaat gtgtaaaatg tggcaatgaa
     7081 ttggaagcca atacaaaatt ctgtgcaagt tgcggaacag ctgtagaaca ggctaaaccg
     7141 gctgctaaag aaacggccga gcagaagaag cctgctgcaa aacctgcaac aaaagctgct
     7201 gataaacctg cggaaaagcc tgctgcaaaa ccggcagaaa aacctgcggc aaagaaagct
     7261 gctgaaaagc ctgctgaaaa acctgcagaa aaacctgcgg gaagtgccgg tttctgcaat
     7321 aactgcggag cggctttgga accgggagca aaattctgtg ccggatgcgg agcgggagta
     7381 ggtgctccgg gagcggcagc gggaccgacg ccttttgctg caatgccacc cgttcaaccg
     7441 cagaaaggtg cgaaggaaac ttttgaagat attaacagga ctccggatac taccgatgag
     7501 tatgatcccg aagaaataga gatgttcaaa cgtatcaccg tagttgcata tctcggctat
     7561 cttgttctta ttccgtggat ttgtttcccg aaaataaaat atgtaagatt tcattcaaat
     7621 caaggtttga tacttgctat tttaaatacc atattcggag ctattacgag tattttaagt
     7681 gccctttcaa gagctcttct tgttaatgtt cgttggctgg gtataatttt ttcaattctt
     7741 accgctataa tttcgcttgc aggcggaata gtaattatat accttattgt tgtaggtatt
     7801 ataaatgcag taaaaggaag agcgaaagaa ttaccgctta tcggaaaaat aagacttctg
     7861 aaataatacg gttagtattt tgaagccgta acggaaaaag gaattcccgg ttgcagaatt
     7921 aaacttctgt aatcgggatt ttttgcttac cgctgaggag tttcttctaa aatattgaca
     7981 tatttatttg ctcatggtac agttgtggaa aataaattca aggagttgtg aatggcggat
     8041 ttatccgtga acatcattat aaacatagta tcggcactgt cagtcatcat tgcagtcatt
     8101 gcgttgtatg ccgtaggtga atcatataag caaagtcagg gggaacaaac cgtataaccc
     8161 gaaaaaagca aaagtgacaa tcctacagaa tacaaagact gtaggatttt ttaaaacagg
     8221 aggaagcttt gaaagcgacg ggaacaaaag taataataga aacgctcata gagcagggat
     8281 gcgacaccgt attcggctat cccggagggg cggtgctgag tatttatgac gaattatacg
     8341 ggcacagtga cagaatccgt catataacct gtgctcatga acagggggct gcgcatgcag
     8401 ccgatgcata ttccagagta acgggaaaag tcggagtggt aatagccact tcgggtccgg
     8461 gcgccaccaa ccttgtaaca ggaatagcta atgcttatct tgactcgtca ccggttgtcg
     8521 ccattaccgg taacgtacct ctttcattgc tgggaaaaga cagttttcag gaagtggata
     8581 taacagggat aacaatgccc gtaaccaagc acaactatat agtgaaggat gtaaacgagc
     8641 ttgccaatct tataagggag gcgtttttta ttgctcaatc gggaagaccg ggaccggtat
     8701 tgattgatgt tcccaaggat gtccagactg cagtctgtga ttttgaacat aagaaagtta
     8761 taaaaaagaa gagagtctgt tcggctacgg aagagcaact tctaaaggcg accgagctga
     8821 tttcccaatc aaagaaaccg tatatatatt gcggcggagg tgcggtaata tcacaggcgg
     8881 gtaaggaaat agcaaaattg gccgaaagaa tagattctcc cataggtttt tccatgatgg
     8941 gtctttcggc agtatcggcc gataaccctt attggctggg aatgacggga atgcacggca
     9001 gatttgcctc cacaaaagca cattccgagg cggatctcat aatagccgcc ggagtacgtt
     9061 tttccgaccg tgcaacgggt aacagatcca aatacacaca aaatgctaag gtcattcata
     9121 ttgatatcga ccccgcggag ataaataaaa atattgagac acattgcagc ttagtcgggg
     9181 atgtcggaac tgttgtcacg aagcttctgg acaaagtgga gaaaaaaaca aatacacaat
     9241 ggatggatgc cgtgcaaaag atgaaagacc taaaggaaat gcggtcttgc aaagattcgg
     9301 taatgcatcc tgaggatatt atcgaaaagg taatggaata tacggatgca agcacaattg
     9361 tagcaaccga tgtaggtcag catcagatgt gggttgcaca gtattacagg tttaaaaaac
     9421 cccgtacgca tcttacttcc ggaggattgg gtgcaatggg tttcggaatg ggagcggcta
     9481 taggaggctg tgtggcatcg ggaggcaaaa gaacggtact gtttaccgga gacggttctt
     9541 tcggtatgaa tcttatcgag ttggccaccg cggtaacata taagttgccg ttggttatac
     9601 tcatattcaa caacggtgtc ttaggtatgg taaggcaatg gcaacatgcg ttttttgaca
     9661 gcagatactc acataccaca ctggacagaa agaccgattt tgtaaagctt gcccaagcat
     9721 tcggggcatc gggatacaga gcaaccgata taaagacgct ggacgaagca ttaaagaagg
     9781 cttttagtac gcaaggaccg gcggtaattg actgtgtgat tgataaagaa gaaaaagttt
     9841 atcccatgat accgcccgag ggatcaatag atgatattat aacaaggtag caagaggtgc
     9901 attatgaaac aaggacaatt catattaagc gtattggtag caaatcatgc cggagttctt
     9961 accagagtat ccggactgtt ttccaggaga aactataata tagacagctt gaacgtagct
    10021 gttaccgaag atccgctctt ctccagaatg accataatag ccagaggaga tcaggcggta
    10081 agggaacaga taaccaagca gttgagcaaa cttcaggatg taaagaagat tgagcttctc
    10141 actccggaaa aaaccgtctt aagagagcac ctgcttataa aaataaaggt aacaaagaag
    10201 acaagaggcg aaatagtgga atcggtaaac attttcagag gcagggtagt ggacttcaat
    10261 attgatgcac tgaccgtgga agttaccggc gaatcggata aactggatgc atttataaag
    10321 ttcgtgtctc agttcggaat tctggagatg tgcagagccg gagcattggc ggtaaaacgc
    10381 ggagccggaa atgataacaa ggaggaagaa taggaagcgt attatggtcg gaagaagaat
    10441 tgaaatttta gattctacat tgcgggacgg tgcccagagt gaaggcatat ctttttccgt
    10501 cagcgacaaa cttaatatag tcaaagcact ggattgcttc ggtatagact atattgaagc
    10561 gggaaacccg gggtccaatc ccaaagatat ggattttttc cagcaggctg cccgactgca
    10621 actgaaacac gctaagttat gtgcattcgg aagtacctgc cgaacaggta tccaccccaa
    10681 ggatgataag aatatccgtg cccttataaa cacaggattg gatacgttgg tgatattcgg
    10741 gaaagcgtgg gatttgcatg ttaagaaagt attgaacata tcgccggaag aaaaccttaa
    10801 aatagtggaa cagaccatat cctatttaaa atcactggga aaatacatta ttttcgatgc
    10861 cgagcatttc tttgacggat ataaatccga tgccgattat gccttaaaag tgttggaata
    10921 tgcggtgaag ggcggagccg atgtattgac tttgtgtgac acaaacggcg gaagcctacc
    10981 cgatgaagcg gaaaagatta caaaggcggt ggtgtcgcta tttcctcatt gtaaaattgc
    11041 gatacactgt cataacgaca ccggatgtgc cgttgcaaac agccttattg ccgtcaatgc
    11101 cggtgccggt atgattcacg gtacttttac gggaatagga gagcggtgcg gaaatgccga
    11161 cctttctata atattgccca atctgaaact taagatgggt tatgaatgta acggagatat
    11221 ttcacaactt tattcggtag ccagaagaat tgcctatata tccaatacac agatcgataa
    11281 taacagaccg tatgtcggaa aatccgcatt tgcacataaa ggcggtatgc atgccgacgg
    11341 agtactgaaa gtgcacagca gttttgagca tgtggatccc gaatcggtag gtaacaaaag
    11401 aaagttctta atgagtgaag ttgcgggcag aagtacgctt attacgaaag cttccgcaat
    11461 agtgcccaat cttaccaaag attcgaagga agtatcaaag atattggaaa aactcaagga
    11521 aatggagcac tacgggtatc agtttgaagc tgcggatgca tcttttgagt tacttgtcaa
    11581 gcgaaccgtg ggattgtata agccgcattt ccatcttgtt ctgtacaaaa caatgagtga
    11641 atttcccgta cctacgggag gaatgcccgc ttcggcaacc ataaaagtgg aagttgacgg
    11701 caaagaagag ataacggcgg taatgggaaa cggtccggta aacgcattgg atctggcatt
    11761 gagaaagtcg ttaaccgtat ttttccctgc cattaaagaa atacagctca tggactataa
    11821 ggtgcgcgta ctggaagaaa acagtactac agcggctaaa gtaagggtac ttatcgaaac
    11881 atccgacggc aagaagagtt ggtcgacaat aggagtatcc aacgacataa ttgaagcaag
    11941 ccttatagcc ttggtggact ctatagagta taagctttcg atgaacgaat aagattataa
    12001 aatagcacta ccgcattttc cgcagaatct ggtgcccggt ttaaagggtg taccgcagtt
    12061 tttgcatacg tttaacggcg gtaaggttgc agatgttgca ggtgctccgc atactccgca
    12121 gaatttggct ccgggtttga acggggtatt gcatctggga caaacattgg acgccgctgc
    12181 tgttgaagca ccgcagtaag cacagaattt tgctcccgca accagcggat tattacatct
    12241 gggacaggta ttggactgct taggggtagc gggttttccg catccggcac agaatttcgc
    12301 tcccggtaca agtgccgcac cgcagtatgc acaggaaacg gaagcactct gcacttttgg
    12361 aacctgcgca ccgcaacccg aacaaaactt tgcattttct ttcaataatg taccgcattt
    12421 agaacaattc ataagctccc ccttgtaatc aaaacattct taatacaatt attttaacag
    12481 atttcaactt gaattcaata tccatgagca ttacgaaaag tccgataggc acgctttgcc
    12541 tttcaaaatg ttcgttgtat atagtttaac tcagcatttc ttcggcataa catatttgtc
    12601 ttttcagttt atcctttttt tcaaagagtt ccgtaacagt gttgatagtc ttctttcttt
    12661 gctgctccat ttcctgcttg atccttgcct ctttcttcat cagatgctta tgtatgaaat
    12721 atgaaacaaa ggaaattgcc gaaatgacaa atccggctat ggaaattccg gttaagactc
    12781 cgactttacc taatataccg gccattaaca ataatacgga aacagcagtg agtgtgatta
    12841 taaaaggaag cgtagtactt ccgcattttc tggcatgatt gttatacctt aaatccgatt
    12901 gtttgatttt gactgctaac atactgattg tgttttgcag gttaaatgtt tccgcttccg
    12961 ttatctttaa ctgatgtgcc cactgctctt tatccaaaga cagttggctg tgaaacttat
    13021 aaacaccctg attatatgtt ctccatgtct gctccaactt atctaaaatg tcggcaggag
    13081 caacattaaa tgtacaattg acaaaatgct ttatatcctc tacatccgtt ctgcttattt
    13141 ccagggtacc gaaatcatct gtttttacac gaactaatcc ccaccagccc ctgtaatcac
    13201 cgggagcatc gttggcaaca ctgtggaaca gctcatgtgc tttgtcaact ttattgtgtt
    13261 tcttgaaaaa tatttccgca tttttaagac gtatttctat agatttctcg tcatgaatat
    13321 gtacatcggc tttttctatt ttatattcat agtatgtttt gtaattgata atggctttct
    13381 cggttacaaa agcggtattg cagttggggc atattgctgc atccttggta ctgtcaattt
    13441 gcagatttgc cccgcattgg gtgcattttg cggctactaa cggcatagga aactccttta
    13501 tttatcattt tgagtgtata caattgctgt caaattgtca acaaaaagcc actgttataa
    13561 tgactgctta ttgtctgttt taacaatgtg aaaatgtgtt attaaaaaag aaccgctctg
    13621 ctgaacggtt ctttcgtgta ttatctcttg tcaatcagat tatggcctgt aatacaaagt
    13681 tgagtatgaa caaggccgaa gcaacccata ataccggtga tatgtcttta aactgtctct
    13741 tgcaaacttt gactatcagg tagaaaatga agcccattgc aatacccgtt gtgatggagt
    13801 aggcaagtgc catgaatata cctgcaaaga aagccggtat tgcttcttcc agttccgacc
    13861 attgaatttc cttgaatgag gaaagcatca ttatacctac taccacaagt gcgggagcag
    13921 ttgccgctcc gggaatggaa ctgataaagg aagcaaagaa tgcgcttaat gcaaaacata
    13981 ttgcaactac aacactcgta agaccggtac gtccgccgac tgctatacct gccgcactct
    14041 ccacataagt ggtggtgttg gatgtgccga atatagctcc gatggatgtt gcggttgcgt
    14101 ctgcaaataa ggctttgtcc attttggatt tgaatcccga gctggattcc attgcttttt
    14161 cgtcttcttc actgaatatt cccgtctgac gtcccgttcc gataaatgtt cctatcgtgt
    14221 cgaaagtatc ggacaagctc attgcgaaaa ttgtaataag aacaatgggc aattttgaaa
    14281 catcggagaa cagtgaaccc agtccctctt ttgtgaatat aacgccgagt gttgtaggta
    14341 acacattaac ggcatcaatg aaacttactt tgtccgcaga tgttgttact cctaacggaa
    14401 taccgataag tgttgcggct actattccga taagtattgc accctttacc ttgagcagaa
    14461 gaagtactat cgtaagaaca agacctataa taaatacata tatagcgccc gtattaatta
    14521 ccgacatgga cggtactccg gcaccgaaat taatggcacc tacattcaat aatccgatgt
    14581 aagcgatgaa tattccgata ccgccgccga tagcattctg aaggctcttc ggtatggctt
    14641 ttataatatg cttacgaatt ttagttaccg tgataagtat gtttattact ccgcagatga
    14701 aaaccataga caatgcatgt tgccatgaaa aaccgtaagt aaaacatacg gtaaatacga
    14761 aaagagcgtt taaccccatt ccgggagcct gtgcatacgg aacattggca acaagaccca
    14821 ttatcagtgt accgattatc gacgcaataa tggttgcaag gaaaacagct ccccattcca
    14881 ttcctgccag cgataacatt gccgggttaa cagcgataat atatgccatt gtaacgaatg
    14941 ttgtcagacc cgccaaaatc tctgttgaca cttttgtgcc attttctttg agcttgaaca
    15001 ttttttaaat cccccttttg aatttaatat actgcaagta tattatatgg tgataaattg
    15061 tcaatataaa agacgtaaaa aacaattcat ttttttaaaa ttccacaata tgtagttata
    15121 tgacatagtc aaaaacgata catattgatt tttgctgtac tcaatgaaat ttgcggttgt
    15181 acagaggatt tttctttaaa aaatttttga aaacagaaaa agaccgtaac tcctgtgaag
    15241 ttacggcctt aggtttttac aacaaatcgg attattgtcc tcttgcgtta aagtcctgac
    15301 agatatagtt cattgcaatg gaaagtctat gcaaataaat gaaaggtcct actatgataa
    15361 gcgatcctaa tacaccccaa agccaatatg tattggaatt gaatgtgtaa gctatgttcc
    15421 tgtgagtaag agcccaaccg acacgattac tcattttgtg tatccagact ataccgccta
    15481 ttcctgctgt aagaggagct atcaacagtg ccaaaagaac aaagtgcatt gttttcttgc
    15541 cgtcatacgg ggaacagaca gtgttcagat ctctgcctac tttgtaaaaa tatacaatac
    15601 cgtatattcc gagcgtgacg atactgagga gaataaatct tcctaaactt ctatttgttt
    15661 gtagtggtgt tacttgcatt acatataccc cctttgttat tttatataac cacctgttta
    15721 tactaaaagt atacgtttat taaaaacttg tgtcaatgtt tttacacaat tttccgttat
    15781 agtcccaatc ttcgcaatga atgtataaaa tacagcattg tggaggaata ccagttaccg
    15841 caagatgaag gctctcccgt aatggggtga agttcctgcc cgaaattcag tttatcggtc
    15901 aggcaccatg ccgttatcca ttttttcatg ttgtcttcca gttgctcgga gaaaccgtat
    15961 ttatccatcc atctcaatga ccgaagagct accagtccct gtgaataaaa gccccatgaa
    16021 ttaccttgtc tgttctgtat ccataacggg tcggatatgg aaaccgcggg gtaggggtaa
    16081 ggagttttga attcttcagt attgtcaagg tatttgctga aaatactttt agccatatct
    16141 acatccaata cattttcaca gaacagattg gttattgaaa ttgatttaca ctttatttta
    16201 ttcccgtgcc tgtcgacatc ataaaaaaag tcatcctgct cgtcataaca tatctccagt
    16261 atttttttct tgacctcacg tgcggcttgt ttccaatcat gagcttcctg ctcaagtccc
    16321 agttttccgg ccattaccgc aagtgccgtc ttagagccgt aaaaaaccgc attcatgtcg
    16381 ggtgttatgt gaggaagcga gtggtcgtca tgcgggtaat cctttgcatc ttcggagatg
    16441 ttgcctttat acttaatatt tgcaaacctt ccgctgttat catgtcccgt gtcaaatccg
    16501 cagaacattt caatcagacc ggttttcatg gtcattctgt tttgggtaag ccagttatcc
    16561 cactttttgc atctgtcata acactccgcc agaagttcca tattattatt ctgtatgcat
    16621 gtctccaagc acagtctggc aaaggacaga cattcctgaa tctggctgta tcctatcttg
    16681 ttcttgaatt tgttaccttt ctcgggatct attacatagc aggggaactg tccgtcgggt
    16741 ttttgcctgt ccagaaacag ccttatctga tgcaccgata cggtcggatc ggttttgtca
    16801 tagtttaccc aggataccgc atcatagaca tgttccaacc atacaccggg gtatgtgtcg
    16861 gatatgagaa agacattgcc ttttgaaccc gccatttttt taagatggta gttatagatt
    16921 ctgtccagaa ccgactcttt cttttcccgt atcaattgtt ttgaaggcat ctgcatttcc
    16981 atttattttc ctcaagtttc ttaattttga ccataatcaa ttctaattaa taactgatta
    17041 tgtgtcaatg aggaccaagt cgaatttgtt accttgaaag tctatgttta tcagccggta
    17101 aacggtaaaa gtgtataata tagataagga gagtgattag caaagaacta tatcataaaa
    17161 tacacatgga atttcaggtt ttaatacatc ccgacgaatt atcatatgag tggattgaca
    17221 gggctatagc aaacgatata gatgcgatag gtattcaccc cgtgggagga tacagggctc
    17281 gggagagtct tgcagatttg gtggaaagat ttaaagacaa tgattttacc gaccttatag
    17341 attatgcatc ggacaacggt ataaaaatag agtacgaaat gcatgcggct tcctacctta
    17401 tgccaagaga gcttttttcc actcaccccg agtatttccg agtagatgaa acaggtcaca
    17461 gaaacaacga tgcaaacttt tgtgtttcaa accaagaagc gttgcaatat gtgagtaatg
    17521 cggcagcaat cctggcatcc ggactttaca aaagttcgga cagatactat ttctggttgg
    17581 atgatgttaa gaataagaag tgtatgtgtg agaaatgccg caatctttcc atgtccgacc
    17641 aacaaatgat attcattaat gctgcattaa aagaaattca aaaaagtaat cggaaagcca
    17701 agatggcata tcttgcatat tttgactgta taacacctcc gcaaaagata aaacctgtta
    17761 aaggagtttt tgcggaatat gctcctatgg ggaaatatgt gtccgaaacg caaacacggg
    17821 aaaactctaa aataaatgaa gaaagcgata tcaacaatct gttttccgtt ttctcaaaga
    17881 gcaactccaa agtgttggaa tattggctgg ataactccat gttttccaac tggaccaagc
    17941 cggagaagaa gtttgttcct gacaataaaa tgataaaaga agatatagag aaatatcatt
    18001 cattgggatt tgaatatatt tcaagctttg cctgctatct cggacctcaa tacacaaagc
    18061 tgcacgggca accggatttg tcggcttttt tccgatgtgt tgagaagtat aaagggtaga
    18121 gataaaaaag atagaaacca agtgtaggga tatatatagg gagagtaaaa aaaagaacac
    18181 ctcacggtgt tctttttggt ggagagagat ggatttgaac catcgaaatc atagataaca
    18241 gatttacagt ctgccccctt tggccactcg ggaacctctc cgtacactgt tttatattgg
    18301 cgacccggat ggggctcgaa cccacgacct ccagcgtgac aggctggcat tctaaccaac
    18361 tgaactaccg ggccaatggt gacccttagg ggactcgaac ccctgttacc gccgtgaaag
    18421 ggcgatgtct taaccgcttg actaaagggc cacaaaaaac agcaatgcgt attttataat
    18481 acatctatat gcaatgtcaa gcataaaatg gcttttgtct tgttattccg gagtatactc
    18541 ttttcgtctg ttgggacagt tttttctttg gcagtactgg cagtttacat aatccttgtc
    18601 tgtaataaac gtcagtccgg ataccgattt gtggggaagc atgagaaaag agggggttaa
    18661 ggtaacacct gtgtctttta tttcttcttt cattatctca aaaagctgtg cctgaccgga
    18721 tataggccag tccactgtgg aaccgggatt gacacttgcg gtttttttaa ggtcatatgt
    18781 attcttcata tcacgattca gacatctgat tgccgtatgt aagagcttct ccattatata
    18841 atctatccaa tagcctacaa aaggatcgga aatgcttttg gaatactcat gtacttcgtg
    18901 accgcaggtg gcgacatagg ggtatacata tccggtatgt cttaagttct ttgcggctat
    18961 tctgctgtta atgataacat cctcaatgcg tacccaatca tcgtctattc cggttatttc
    19021 ctttttggta aaaagatatt tgggacatgc gatgcttatg cacttatcat atacttcatt
    19081 gaattcatca atatcatctt ccgatattct taatcttttt atgatatctt ctttgtcaat
    19141 cgtataatcg tctttgctta atttaataac cataaccgcc tccggcatca tcaaagaata
    19201 ttatacatgt tttgtaataa aatttcttct ttttagttaa tatggcatat taaggatgaa
    19261 taacccttga gtgcaaagtt aaattccagt ataaaggagc ggcactggat tttaatctt
//
LOCUS       DAOSOM010000035        17636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_3233_length_17636_cov_5.547125, whole genome shotgun sequence.
ACCESSION   DAOSOM010000035 DAOSOM010000000
VERSION     DAOSOM010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 17636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17636
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3233_length_17636_cov_5.547125"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..185)
                     /locus_tag="PLF14_07865"
     tRNA            complement(97..185)
                     /locus_tag="PLF14_07865"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(148..150),aa:Ser,seq:cga)
     gene            491..772
                     /locus_tag="PLF14_07870"
     CDS             491..772
                     /locus_tag="PLF14_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009219357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HOT71237.1"
                     /translation="MSTTNINIRVDSSLKKEAEDLFRDLGLNMSTAITIFLRSAVSYN
                     GIPFEVRRLGPNPETEAALAEYEEMKKNPGNYKRYNSFKEAMDEVLSDA"
     gene            765..1070
                     /locus_tag="PLF14_07875"
     CDS             765..1070
                     /locus_tag="PLF14_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HOT71238.1"
                     /translation="MLKAALSNQFKKDLKQAKKHGFKLEKLNKVVNMLARQEPLPAKH
                     RDHNLTGKYRNFRECHVEPGWLLIYRIENSELVNHFRVYIFFDIESLFRYNMVHGHS"
     gene            1061..1137
                     /locus_tag="PLF14_07880"
     tRNA            1061..1137
                     /locus_tag="PLF14_07880"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1095..1097,aa:Arg,seq:cct)
     gene            1160..1420
                     /locus_tag="PLF14_07885"
     CDS             1160..1420
                     /locus_tag="PLF14_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOT71239.1"
                     /translation="MVRKNSYTTVIRRLDPLGRIVLPKKYRKKCEMNVKDDVEMYLEG
                     SAICVKKYNTRCKLCGETDNLIEFKGANLCPACVTGIKRLNF"
     gene            1420..1875
                     /gene="tadA"
                     /locus_tag="PLF14_07890"
     CDS             1420..1875
                     /gene="tadA"
                     /locus_tag="PLF14_07890"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004798784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HOT71240.1"
                     /translation="MLKKMMTEALEQASMALEHDDVPVGAVIVKDGNIVAKAHNRRFC
                     DKDSTAHAEILAISQACAFFNTQFLDGCDIYVTCEPCIMCAGAIIQSRIENLYFGAYD
                     KKAGCAGSVLNVFDTAFNHKVSVTGGIMEAECSKLLSVFFKNKRIRKNE"
     gene            2017..3615
                     /locus_tag="PLF14_07895"
     CDS             2017..3615
                     /locus_tag="PLF14_07895"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOT71241.1"
                     /translation="MLGNKIHELRKKHSLTQEAFASLINVSRQAVQKWERGISLPDCD
                     KLISISRVFNVSVDYILDLETRDPHQEFRVKKEIQPSYQALHWWEVYSTQLSIEYKQC
                     LDEGKDITKLKTLMESIQALETSTYKEQLADTLHNMILNTPYAPDYPYNEPDELNLIY
                     PLTKHDFHYPFVKLSPNVLKSKIKGAWMGRICGCLLGKTVEGIRTEELVPLLKETGNY
                     PMHRYILKSDMSEEIYSKYKYNLRSRCFADAIEYAPSDDDTNYTVLSSVIVDKFTDRF
                     TSYDVAKTWLEKQPKSAYCTAERVAFLNIAKGYLPPETAIYKNPFREWIGAQIRVDYY
                     GYINPGDPKKAAYMAWKDARVSHVKNGIYSSMLIAAIIASAACCNNVKDAIYYGLYQI
                     PQTSRLYKAVMKVLKQYESGMTCEKCFESIHEEYDEHSGHHWCHAISNTCVVIASLLY
                     GNNDYAKSICLAVQTGFDTDCNAATVGSVIGMLKGIEYIPDYWTAPIKNKLETSIFGI
                     GIVDTKYLVDITYKHLMSYSDIYK"
     gene            3777..4427
                     /locus_tag="PLF14_07900"
     CDS             3777..4427
                     /locus_tag="PLF14_07900"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOT71242.1"
                     /translation="MFCPKCGTNNGDNTRFCFNCGYALSAPQQPNQQLPPLQQPNRYQ
                     QQLPPLRQPNQFQQMPPLQQAPYGRPYVQPKKSKVGVVIAVIAIIVVLGLLVAALYNG
                     WFSSAPGIKKACVATALDPETYEPLNQVRNVSSTEPTIYVAAYIQNVNQGVNVVSVWY
                     HLDSDESFISDYMYIDYDAWFYFCIDQSEFDFPVGDYVVELYIDDYLEETVEFTIY"
     gene            4744..6273
                     /locus_tag="PLF14_07905"
     CDS             4744..6273
                     /locus_tag="PLF14_07905"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOT71243.1"
                     /translation="MKKDFDKKDRLIDDMTENNSNRIYRSVNTSEEETKRLLALNEEL
                     EEKKEQLQIILNSTAEAIYGVDTEGICTFCNKSCLRILGYETEDRLLGKKMHELLFDS
                     TDERTCKVIEYMKKGRALHIDNIKFLKADKSTIHVELHSYLQYKNDKVIGAVVTFLDI
                     SQKISDMECIRYLSYHDVLTDLPNRRFFEKKIKEVDVSDNLPYSVIFADINGLKITND
                     IFGHDIGDRLILRSAHAMKNNVSKDTFIARVGGDDFIVLLPKTDREEAYDIMNAVKEE
                     IHNARIAAITCSISIGSETITDNKTSIDQAINMAEDRMYREKVMNRRSTNTDMLNELV
                     VTLHKRSPREVVHSKNVSELSEKIASAMKLPEPLVKKAKIAGYMHDIGKVVLDENILK
                     KEIYTDEEREQFHLHPVYGYRILNLFDDMLDIAEGIYSHHEKWNGSGYPKGLKESDIP
                     LIARIVAVAEAYDALTNTKNICALSKEEALGRIKESSGVTLDPYIADLFVDIMQKSVT
                     V"
     gene            6407..7474
                     /locus_tag="PLF14_07910"
     CDS             6407..7474
                     /locus_tag="PLF14_07910"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOT71244.1"
                     /translation="MLGGKNKVLRRYLSNNNRFKSDNPDYGRAFLLNAALAYMSVICM
                     IFGILHIIKTDHPIFIYFYFAVMILSIVVIIFFHKTDNVKLTSLFAVILATFIMGSIL
                     HLVYSDYSGLFWAAAYLPFVYLMLEGKKGVRATLILGVSGLLYIIFFHRDWQPGIFNY
                     MSIINIAGAGACLMLLIVYYERSQKRAEKSLVAKNNQLHLMSITDRLTGLYNRHKLDE
                     TLNELVCKSKTCKKPFSIIIADIDRFKSINDTYGHLTGDKVLVEISNLLLACVRQTDI
                     AGRWGGEEFLIICPDTDYAGITDIAKRIEAFISGYNFGCCQGVALSCGVATHNSGETV
                     DSLLQRADTNMYQTKQSRRNT"
     gene            7520..8191
                     /locus_tag="PLF14_07915"
     CDS             7520..8191
                     /locus_tag="PLF14_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010299398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT71245.1"
                     /translation="MYKILIVEDDLVIASSLKKHIETWGYEAQIVSDFKNVFDCFMDF
                     KPHLVLLDISLPYFNGYHWCEKIRKVSKAPILFLSSFSDNMNIIMAMNMGADDFIAKP
                     FDIDVLTAKINALLRRAYDFKPDDDFIAVNGLLLNTNNLTVEYEGIKSELTKNEFRIL
                     QVLMSNSGKAVSRDMLMTKLWETDSYIDDNTLTVNINRLRRKLESMGLVDVIKTKKGV
                     GYLIS"
     gene            8188..9180
                     /locus_tag="PLF14_07920"
     CDS             8188..9180
                     /locus_tag="PLF14_07920"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOT71246.1"
                     /translation="MKLLYEYLKYHIKSIILFVVFTIIFAIVLHLYSLPLEPVLYAAI
                     LCLVLAVIITLISFFRFRTKHIALLKMIDNISVSKNGLPAPKNIIEEDYIKLIDVLHK
                     EKCEIDSQKSLTISELNDYYTLWVHQIKTPISAIKLLFKSKGINDIELEQQLFKIEQY
                     AEMALQYIRSESDTTDFVIREYSLDAIIRQAIRKYSKLFILKKISLNYTPTDAVVITD
                     EKWLLFVIEQILSNSLKYTQKGTISIYMDDRYDLTLIIEDTGKGIEPEDIPRIFEKGY
                     TGYAGRIDKKSTGIGLYLSKKILSRLSHKIRIYSVPDKGTKVLIDLSYSDKISE"
     gene            9286..10065
                     /locus_tag="PLF14_07925"
     CDS             9286..10065
                     /locus_tag="PLF14_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT71247.1"
                     /translation="MTVTNMILDVRNIKKVYTTRFGGHQVQALSNVTFSVEQGEFIAI
                     MGESGSGKTTLLNILAALDKPTSGEVFLDGNALTGISEKEISAFRRNNLGFVFQDFNL
                     LDNFTVKDNILLPLVLSGKTYDEMVPILKPVLNKLGIEDITEKYPYEISGGQKQRTAI
                     ARAIITNPKLLLADEPTGALDSKATNSLLKLFRKINEDGQTILMVTHSIKAASHASRV
                     LFLRDGAIFHQIYRGESTNEELYQKISDTLTILATGGIFNA"
     gene            10058..>11960
                     /locus_tag="PLF14_07930"
     CDS             10058..>11960
                     /locus_tag="PLF14_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016207890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT71248.1"
                     /translation="MRNSMYFKLAASNIRKNGKTYIPYLIMSITTIMMFYIMVFLVYN
                     PGLSGMRGERHIKTVLSLGCYITGAFSAVLLFYTNSFLFKKRKKEIGLYNILGMEKKH
                     IAKMLTIENMYSYLVTIVLGLFFGILFSRLMLLILLNILKAGTGFVFNISIEGLCITF
                     VFFTIVSALILLNNIRQVHLTKPVELLKGSSLGEKEPKTRWLLAIIGVLSTGAGYYIA
                     LSVENPLAALNIFFLAVILVIIGTYCLFTAGSIALLKLLRKNKKYYYKTNHFTAISGM
                     IYRMKRNAVGLANICILSAMVLVTVSSTVCLYVGMHDALNNRFPTDIEIITHNADKEQ
                     SVSISNAVNKIVSESGYEISKYSEFRYLSHSMLYEEEKDTFSICFDINKNSDNMYYVY
                     FFPVEDYNKKTGTDILLEDKEVLIYNRNNVDFTGNLNIQSIEFIVKGNVKDPGFPMSV
                     DGFNKVYLFFKDEQVIIDINKQFEEYFGAYSSLTHTIAFDVMGDKAEQLKLTDRIKVT
                     FERLEENISINTISKAEEEYVFYAIYGGLFFLGLFLGVLFMMATVLIIYYKQISEGYE
                     DKQRFHIMQQVGMTKAEVRQSIRSQVLTVFFLPLIMAIIHIVFAFRMITKILGVLYLT
                     NVPLFINCTV"
     assembly_gap    11961..11970
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12086..13315)
                     /locus_tag="PLF14_07935"
     CDS             complement(12086..13315)
                     /locus_tag="PLF14_07935"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOT71249.1"
                     /translation="MFFDIVEKTDKYLEYMDYFILGPSVVRCIHSDDLLMNFDLFNAS
                     DSNAGSVLLRSKDNGNTWIEQGLIEKSYVYDFNNSRVKSGYGALYCDTDAKVILYVAN
                     DTYWEENKIASLWRMRTLYYRLSFDDGYTWTDKKYIVQKGDCYNTKHFMKSVEFGVNM
                     AATVSPLIVRAKDNSLLVPVQVQMLNKDGTLFNPTGMGYMKAGALKAAWNSEKLEYEW
                     DLGEYVTVEPDKSIRGLYEPAFGIIDGKENEILMVMRNSNYTQSDSIIGAKFYSISED
                     YGYTWSDPEMLLYDDGSIMYSSSSIPKLLNHSNGNLYFIGVINRENPKGNLPRYPLCI
                     AKVDKETKRIIKKSVTVIDTKREHHNLSVKTSNVVDYSNHGVYEDKETGNIVVLAPYR
                     EDLTKYPCYLNKYVIKV"
     gene            13628..14113
                     /locus_tag="PLF14_07940"
     CDS             13628..14113
                     /locus_tag="PLF14_07940"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT71250.1"
                     /translation="MVELKKITWENFEQCLSLGVTDNQQDFVEDNVYALAQAYVAATN
                     DKFPPIAYAIYNDETVVGIVILVHKYAKDSKYYHENCYEVVSFMIGLQYQGKGYGKEA
                     FAKVIEVIKTFPEGKAKAICLSYDPHNDTARHLYASYGFIETGVINDLGEVVAKLPIA
                     T"
     gene            14246..15616
                     /locus_tag="PLF14_07945"
     CDS             14246..15616
                     /locus_tag="PLF14_07945"
                     /inference="COORDINATES: protein motif:HMM:NF025644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHHW family protein"
                     /protein_id="HOT71251.1"
                     /translation="MRRIKLDTLIFVVFITVFGLLNLLNPDKSEISLQENRTLAKKPV
                     FSFQSVFDKSYASGWNDYYSDTLLLRENLIQANVQIKKNFGIEKNRISISVIGTEPAP
                     TKTPKPTQTPVASVSPVPTETQAHNPETSDEPSASPSPSPAPTPVEEEPTGSGAATGM
                     YITIGDETFKLDTYSEEAYDNYANYINKIKKMAGPDIEVYSMLAPSRSTYLALTKYDF
                     LSSRAANVEQINSKIDKSVKNVNVLRSMVEHVDEYIYYRTDHHWTHLGAYYGYTELMK
                     ARNRQSEIIPLSKYSEKLVVEGYLGFYNKPYLTQADYDNPDTIIAYYPIVDYKYIYNS
                     QDVSIEKRLINKERVKPDKDYYLLFSDGGFGTYSEITTSSDSDLKCMVIKDSFGDSLI
                     SFLLPHYKTIYVVDSRTYNKAFCGNMNIIDFAKSKKVDEIIVNYWITEITYMDDFMKK
                     AYATLE"
     gene            complement(15617..16147)
                     /locus_tag="PLF14_07950"
     CDS             complement(15617..16147)
                     /locus_tag="PLF14_07950"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOT71252.1"
                     /translation="MHDYILIRSRRRTISLRISQDCTVTVRAPVFCSKKVIDKFVSDH
                     EKWIDRQIEIKKNRPSVKDFTDEQIKELKKQAKQVLTERTKYYSEITGLYPAYIKITS
                     SERRFGSCTQKGGICYSYRLILYPERAVDYVIVHELCHLRHRNHQKPFYDLVKKYMPD
                     YKERDKLLKNATGIPL"
     gene            complement(16191..16415)
                     /locus_tag="PLF14_07955"
     CDS             complement(16191..16415)
                     /locus_tag="PLF14_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71253.1"
                     /translation="MDALFPLNKNVKKKKLGTLVIAILLYVVLGVVVGLIQWLLRGIP
                     VVNTVMSIVRYLVWLYSVVGVILAIIKFFK"
     gene            16552..17130
                     /locus_tag="PLF14_07960"
     CDS             16552..17130
                     /locus_tag="PLF14_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOT71254.1"
                     /translation="MTFFELLLIAIGLSMDAFAVAICKGLSLKKVTWKDALTVGLYFG
                     IFQAAMPLAGFLIGSAFAGIIDKWDHWVAFTLLAVIGIKMIIESKKRGSCRSSEDNSQ
                     SLSFKQMLPLAIGTSIDALAVGVSFALLSIKILPAVSFIGAVTLILSFAGVYAGSIFG
                     CRFKSGAEITGGIILILTGLKILLSHLGIIPF"
     gene            complement(17244..>17636)
                     /locus_tag="PLF14_07965"
     CDS             complement(17244..>17636)
                     /locus_tag="PLF14_07965"
                     /inference="COORDINATES: protein motif:HMM:NF017007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HOT71255.1"
                     /translation="SEIYKKEEYEQSLTVSYKRDMSAKADIYFMTNQKFFPAEKLYAV
                     KEKLDSMTDEQIQRLMFVQMKDPTTYLLVSIFIGQLGVDRFLMNDIGMGILKLLTGGL
                     CGILWIYDMVTIQDKVRGMNFDLFMNFM"
BASE COUNT         5681 a         3307 c         3421 g         5217 t
ORIGIN      
        1 actacgcccc ttgtggactt tcaccacaga ctgacaacat gcccgtcata cacgaaaaac
       61 gaactttgac caatcagaca aaagttcgtt cctgtatggc ggaggaggtg ggattcgaac
      121 ccacgtgtcc tctcggacaa ctcgatttcg agtcgagctc gttatgacca cttcgatact
      181 cctcccctcg ccttatatat attacttaaa taaatcattt aaatcaatag tttaagtaag
      241 gagtgtttcg gtttttagtt gtagaaattt tgaatgataa ttatatctgt gtggatacat
      301 tgttatctct cgcagcaagt tgccggcaag ttaaattacg tctaatttaa aatattcagt
      361 tcagataaac atactgaata cttaactcag gagaaacagt aacgcaaaca atgctttaca
      421 ctgtgctgac atctcttgca ccgagattat aataatgctg caataaaaag aaggatagga
      481 ggtgacggct gtgtccacta caaatatcaa tatcagagta gattcttctc ttaaaaagga
      541 ggccgaagat ttgttcaggg acttaggtct gaatatgtcc accgccataa caatattcct
      601 aagaagtgca gtcagttata acggcattcc tttcgaagtc agacgcttag gtccgaaccc
      661 tgaaactgaa gcagccttgg cggagtacga agagatgaag aaaaatcccg gcaactataa
      721 acgctacaat tcctttaagg aagctatgga tgaggtactc tccgatgctt aaagcggctc
      781 tgtcaaatca attcaaaaaa gatctgaagc aagcaaaaaa acacggtttc aaactcgaaa
      841 aacttaataa agttgttaat atgcttgccc gccaggaacc actaccggca aagcaccgtg
      901 accacaacct gaccggaaaa tatcgcaatt tccgtgaatg tcatgtagaa cctggttggc
      961 tcttaattta ccgaattgaa aattcggaac ttgttaatca cttccgtgtc tatattttct
     1021 ttgacattga atcgctgttt aggtacaata tggtgcatgg gcactcatag ctcagcagga
     1081 tagagcgtcg gtttcctaaa ccgcaggccg gaggttcgaa tcctcttgag tgcaccagtt
     1141 ttaataggag gcaacataaa tggttagaaa aaattcatac actactgtta tcagaaggct
     1201 ggacccgttg ggaaggatag tactgcctaa aaaatacagg aagaaatgtg aaatgaatgt
     1261 caaagatgac gtcgaaatgt atcttgaagg ttccgctata tgcgttaaga aatataatac
     1321 ccgttgcaaa ttgtgtggag aaaccgataa cctgatagaa ttcaaaggag caaacctgtg
     1381 ccctgcttgt gttacgggaa taaagagact taatttctga tgttaaagaa aatgatgacc
     1441 gaagctttgg aacaagcatc catggctttg gagcatgacg atgtaccggt cggcgctgtt
     1501 atagtaaaag acggcaatat agttgcaaaa gctcacaaca gaaggttttg cgataaagat
     1561 tccactgcac atgcggagat tttggcgata agtcaggcat gtgctttttt caatacgcag
     1621 tttcttgacg gttgtgatat ctatgtcacc tgcgaaccct gcataatgtg tgcgggagca
     1681 ataatacagt caaggattga aaacctgtat ttcggtgctt acgataaaaa agccggatgt
     1741 gcgggatcgg tactcaatgt ctttgatacc gcttttaacc ataaggtgtc ggtaaccggc
     1801 ggaattatgg aagcggaatg ttcgaaatta ttgagtgttt ttttcaaaaa caaaagaatc
     1861 aggaaaaatg aataagttgg cacagtacat catttaattt caagctatat gtatagatat
     1921 atagtcttta tgctacttac agttgcataa atgtacacta aagtatattg aactctctct
     1981 ttgtatatcg tattattgac atacggaggt gttattatgc tcggtaataa aattcatgaa
     2041 ttgcgaaaaa aacacagtct tacacaggag gcatttgctt cactgattaa tgtaagcaga
     2101 caggctgtac agaaatggga aagaggcata tctttaccgg actgtgataa actcatatcc
     2161 ataagcaggg tttttaatgt atcggtggac tacattctgg atctggaaac gcgggatccg
     2221 catcaggagt tccgtgtaaa aaaagagatt caacccagtt accaggcttt gcactggtgg
     2281 gaagtatatt ctacacaact ttcaatagaa tacaagcaat gtcttgacga aggcaaagat
     2341 attaccaagt tgaaaactct tatggaaagc atccaggctt tggagacatc cacttataaa
     2401 gaacaactgg cggacacgtt gcataatatg attctgaata ctccctatgc accggattat
     2461 ccgtataatg aacccgatga gcttaatttg atttatcctt taaccaaaca cgatttccac
     2521 tatcctttcg ttaagctttc accgaatgtt ttgaaaagta aaatcaaagg agcatggatg
     2581 ggccgcattt gcgggtgtct gctgggaaaa acggtggaag gtatccgcac ggaggaactt
     2641 gtcccgctgc ttaaagaaac gggtaattac cccatgcaca gatacattct caagtcggat
     2701 atgagtgaag agatttattc gaaatacaag tataatttaa gaagcagatg ttttgccgat
     2761 gcaattgaat atgctccttc ggatgacgat accaactata cggtactctc ctccgttatc
     2821 gtagataaat tcaccgaccg gtttacctca tatgatgtag caaaaacctg gctggaaaaa
     2881 cagccgaaga gtgcttattg cacagccgag agagtagctt ttctgaatat agcaaaaggt
     2941 tatctgcctc cggaaaccgc aatttacaaa aatccgttca gagaatggat aggtgctcag
     3001 ataagggtgg actactacgg gtatatcaat cccggagatc ctaaaaaagc ggcatatatg
     3061 gcatggaaag atgcaagagt atctcatgta aagaacggca tatactcatc aatgctgata
     3121 gctgcaataa tagcctcagc ggcttgttgc aataatgtga aagatgctat atattacggt
     3181 ctttatcaaa taccccagac atccagactg tacaaagcag tcatgaaggt attaaaacaa
     3241 tatgaatcgg gaatgacttg tgaaaagtgt tttgaatcta tacatgaaga atatgatgaa
     3301 cattcgggac atcactggtg tcatgccatt tccaatacct gtgtagtaat agcttcgctc
     3361 ctgtacggta acaatgatta tgccaaatca atatgcttag cagtgcagac agggtttgat
     3421 accgactgta atgccgctac ggtaggttcc gttatcggaa tgctcaaagg aatagagtat
     3481 atacccgatt attggaccgc tccgataaaa aataaactgg agacctccat tttcggaatc
     3541 ggtattgttg acacgaaata ccttgtggat ataacgtata aacatctgat gagctattcc
     3601 gatatttaca agtagcggta aaaatataaa ccccggtaag tcatatatat aatagtagta
     3661 tttcttattg ccgatttaat attgttcatg ataaaattat gttgtatagc agatatataa
     3721 tgtaatacag tgactatttc ataaaattaa aagaaaaata caaagggggt agtgaaatgt
     3781 tctgtccgaa atgtggtaca aataatggtg ataacacgag attctgtttt aattgcggtt
     3841 atgctttgtc cgcacctcaa cagccgaacc aacagttgcc gccactgcag caacctaacc
     3901 gatatcaaca acagttaccg cctctcaggc aacctaacca attccaacag atgccgcccc
     3961 tacagcaggc accgtacgga cggccatatg tacaacctaa gaaaagtaaa gtaggtgtcg
     4021 taattgccgt aattgccatc attgtagttc tcggtttgct cgtagcggca ttatacaacg
     4081 gttggttttc ttctgctccc ggaattaaaa aggcatgcgt agctacagcg ctggatccgg
     4141 aaacttatga gcctctcaac caggtcagaa acgtatccag taccgaacct accatttatg
     4201 ttgcggctta tatacaaaat gttaaccaag gcgtaaatgt tgtctccgtt tggtatcatc
     4261 ttgattcaga cgaatctttt atttccgact atatgtatat tgactatgac gcatggttct
     4321 acttctgtat agaccagagt gagtttgact tccctgtcgg agattatgta gtggaattgt
     4381 atattgatga ttatctggag gaaacagtgg agtttactat ctattaacaa caacagttac
     4441 ccatatggat gttaatataa atattcttat ctgatagcgg tgtctataaa aagcgactga
     4501 aatacagcga agcaatacgc ttttcaataa acattttttt acatttttct ttatttctat
     4561 cttttttgtg atataatctc taatggtcaa ctatgacaaa aattataaaa aacatggctg
     4621 cataaagcag tcctatatat ataagtattg gttaccggcg aattatttga taattaaaat
     4681 atggacttgt gtattacctc ttctcaaact gatccgccga cgtaactaaa tgagttatgg
     4741 attatgaaga aagattttga caaaaaagac agattaattg atgacatgac tgaaaataac
     4801 agcaaccgga tataccgttc cgtgaatact tcggaggaag agaccaaacg actcttggct
     4861 ttaaatgaag agttggaaga gaaaaaagaa cagttacaga taatactgaa ctcaactgcg
     4921 gaagctattt acggggtaga tacggaaggg atatgcactt tctgcaacaa aagttgtctg
     4981 agaatattgg gttatgaaac cgaggaccgg cttcttggca aaaaaatgca tgaactgctg
     5041 tttgacagta ccgacgaaag gacatgcaaa gttatcgagt acatgaaaaa aggcagagct
     5101 ttgcatatag ataatataaa gttcttaaaa gcggataaaa gcactattca tgttgaactg
     5161 cattcttatc ttcagtataa aaatgacaag gttataggtg cggtggtaac tttcctggat
     5221 atatctcaaa agatatccga tatggagtgt ataagatacc tgagttatca tgatgtgctt
     5281 accgatcttc ccaacaggcg gttttttgag aaaaaaataa aagaggttga tgtatcggat
     5341 aacctgccgt attcggtaat atttgccgat ataaacggtc ttaagataac caacgatata
     5401 ttcggacatg atataggtga cagattgatt ctgagatcgg cacatgcgat gaaaaataat
     5461 gtgagcaaag atacattcat agcccgtgta ggaggtgatg attttattgt actattacct
     5521 aagaccgatc gtgaagaagc atacgatata atgaatgcgg ttaaagagga aatacataac
     5581 gccagaatag ctgctataac atgcagtatt tccataggat cagagacaat caccgataat
     5641 aaaacatcca ttgaccaggc aatcaatatg gccgaagaca ggatgtatcg tgagaaagtc
     5701 atgaacagaa gaagcaccaa taccgatatg ctgaatgaat tggtcgtaac cctgcacaaa
     5761 agaagtccca gagaagtggt ccattccaag aatgtatcgg aattatcgga aaagatagca
     5821 tcggccatga aactacccga acctctggtt aaaaaagcaa agatagcggg atacatgcat
     5881 gatatcggga aagtggtact tgacgagaat atacttaaaa aggaaattta cactgatgag
     5941 gaaagagaac agttccatct acaccctgtt tacggataca gaatattaaa tctttttgat
     6001 gatatgctgg acatagccga aggcatatac tcccatcatg agaaatggaa cggttcaggg
     6061 tatcccaaag gcttgaaaga atcggatatt cctttaattg caagaattgt tgcggttgcg
     6121 gaagcatatg atgcgctcac taacactaaa aacatctgtg ctttatcaaa agaagaagct
     6181 ttgggcagaa tcaaagaatc ttcaggggta acacttgatc cctatatagc cgatttgttc
     6241 gtggatatta tgcaaaagag tgtaacagta tgaattccgg atttaaaaat aatagagaga
     6301 aaatatttac cttaatttta ccttgaatta ccaataatgc atatttttgc ttgcacttta
     6361 tttacagata gtgtatatta tgctggaatg attatttaga aggtgtatgt tgggggggaa
     6421 aaataaggtt ttaagaagat acctttcaaa taataataga tttaagtctg ataaccctga
     6481 ttacggaaga gcattccttt tgaatgcagc acttgcatat atgtcagtta tatgtatgat
     6541 tttcggaatt ctgcatataa ttaaaaccga tcatcctatt tttatctact tctattttgc
     6601 ggtaatgata ttgtcgattg ttgtaatcat attcttccat aagacggata atgtaaagct
     6661 tacttccctt tttgcagtaa tattagcaac ttttataatg ggttccattc tccatctggt
     6721 ttacagcgac tattccggct tattctgggc tgcggcatat cttccgttcg tgtacttgat
     6781 gcttgaagga aagaaaggag taagggcaac actgattctg ggagtatcgg gacttttata
     6841 cattattttt ttccacaggg actggcaacc tggcatcttt aactatatgt caattatcaa
     6901 tattgcaggc gccggtgcat gcctgatgtt actgatagtg tattatgaaa gaagccaaaa
     6961 acgggcggaa aaatctcttg ttgccaaaaa caatcaactt catctgatgt ccataacgga
     7021 taggctcacc ggtctttata acagacacaa actggatgaa acactgaatg aactggtatg
     7081 caaaagtaaa acctgcaaaa agcccttttc catcatcatt gcggatattg acagattcaa
     7141 atccataaat gacacatacg gacacctgac aggcgataaa gtgcttgtgg agatatccaa
     7201 tcttctgttg gcttgtgtaa gacaaacgga tattgcagga cgctggggcg gtgaggagtt
     7261 ccttataatc tgtcccgaca ccgattatgc aggtataacg gatatagcaa aaagaataga
     7321 agcatttatt tccggatata acttcgggtg ttgccaggga gtagcattaa gctgcggagt
     7381 tgctactcac aacagcggtg aaacagtgga ttctctgctc caaagagcgg atacaaacat
     7441 gtatcagact aagcaatccc gcagaaatac ctgacaactg tttttatggt aaaataaagt
     7501 acggtaaagg aactcaaaca tgtataagat acttattgtt gaggacgatt tagttattgc
     7561 ttcttcatta aaaaaacata ttgagacatg gggctatgag gctcagatag tgagtgattt
     7621 taagaatgta tttgactgtt tcatggattt taaaccgcat cttgttcttt tagacatttc
     7681 acttccctac tttaacggtt atcactggtg tgagaagata agaaaagttt ctaaagcacc
     7741 tattcttttt ctttcttctt tttccgacaa tatgaatata ataatggcga tgaatatggg
     7801 agcagatgac ttcatagcaa aaccttttga tattgatgtc ctgactgcta aaatcaatgc
     7861 acttctcagg agagcatatg attttaagcc tgatgatgat tttatagcag ttaacggcct
     7921 attgctcaac actaataatt taacagttga atatgaagga ataaaaagtg agcttactaa
     7981 aaacgagttt cgcattttac aggtgcttat gagtaacagc ggcaaagcgg tttcacgcga
     8041 tatgcttatg acaaaattgt gggaaacgga cagttatata gacgataata ctttgactgt
     8101 taatattaac agattgagga gaaaacttga aagcatggga ctggttgatg ttataaaaac
     8161 caagaaagga gtcggttacc ttatttcgtg aaattgttat atgaatacct gaaatatcat
     8221 attaaaagta tcattctgtt tgtcgttttt acaattatct ttgcaattgt attgcacctc
     8281 tactctcttc cccttgaacc ggttctgtat gccgccatat tgtgtctggt actggctgta
     8341 ataataaccc tgataagttt tttccgattc cgaacaaaac acattgctct gcttaagatg
     8401 atagacaata tttccgtaag caaaaacggt cttcccgctc ctaagaatat tatagaagaa
     8461 gattatatta aacttattga tgttttgcat aaagagaaat gcgaaataga ttcacaaaag
     8521 tcgcttacca tttccgagtt gaacgattat tacactttat gggtacatca aatcaaaaca
     8581 cctatatcgg caatcaagtt gttgtttaaa tcaaaaggga taaatgatat tgaacttgag
     8641 cagcaactct tcaaaattga gcagtatgct gaaatggctt tacaatacat aagaagcgaa
     8701 tcggacacga ccgattttgt tatacgcgag tattctttag atgcaattat acgtcaggca
     8761 ataaggaagt attccaaatt attcatcttg aaaaaaatat ccctgaatta caccccgacc
     8821 gatgcagttg taataaccga cgaaaaatgg cttttgttcg tgatagaaca gattctatcc
     8881 aattctctta agtacacaca aaaaggtact atatcaatat atatggatga caggtatgac
     8941 ctaactctta ttattgaaga taccggaaaa ggaatagaac ccgaggatat tcccagaatt
     9001 ttcgaaaagg ggtatacggg gtatgccggt aggattgaca agaaatctac gggtataggt
     9061 ctttatttaa gtaaaaaaat actgtcaaga ctatctcaca aaattcgcat atattcagtt
     9121 cccgataaag gcaccaaagt tcttattgat ttatcgtata gcgacaaaat atccgaataa
     9181 cttacaaaaa tgtaagatag gattgttttt tgtaagcaac tgttatggtt aatcgtgcaa
     9241 tatcctgtaa taattgtatt gcggatattt catatggagg aaaatatgac tgtgacaaat
     9301 atgattttag atgttagaaa tattaaaaaa gtttacacaa ccagattcgg aggacatcag
     9361 gttcaagcct tatcgaatgt gacattttcc gttgaacaag gggaattcat tgcgataatg
     9421 ggcgaatcag gttccggaaa aaccactcta cttaatatat tagcagcttt agacaaacct
     9481 acatcgggcg aagtattctt agacggtaat gctcttacag gtatatcgga aaaggaaatc
     9541 tccgcattca gacgcaacaa cttgggattt gttttccagg atttcaattt attggacaac
     9601 tttaccgtta aagataatat tcttttacct cttgtcttat cgggcaaaac atatgatgag
     9661 atggttccca tactcaagcc ggtactgaat aaattaggaa tagaggatat aacggaaaaa
     9721 tatccttatg agatatcagg cgggcagaaa caaagaacag ctatagcacg ggcaataata
     9781 acaaacccga aactgttact cgccgatgaa ccgacaggag ccttggattc caaagcaact
     9841 aattcacttt taaaactttt ccgcaaaata aatgaagacg gacagactat tctgatggtc
     9901 acccattcca taaaagccgc aagccatgca tccagagtgc tttttttgcg tgacggagcg
     9961 atttttcatc agatatacag aggagaatcc acaaatgaag aactgtatca gaaaatctcc
    10021 gacactttga ccattttagc aacaggaggc attttcaatg cgtaattcca tgtatttcaa
    10081 gcttgccgca tctaatatca gaaaaaacgg caagacttat ataccctact taattatgtc
    10141 aatcacaact attatgatgt tttatataat ggttttttta gtgtataatc cgggtttgtc
    10201 cggtatgaga ggtgaaagac acattaagac cgtactctca ttagggtgtt atataacagg
    10261 ggctttttcc gctgttctgc ttttttatac aaacagtttt cttttcaaaa aaagaaaaaa
    10321 agaaataggc ttatacaaca ttctgggtat ggagaagaaa catatcgcca aaatgcttac
    10381 tatagaaaac atgtactcat atttggtgac tatagtatta ggtcttttct tcggtattct
    10441 tttttcccgc cttatgctgc ttatactgct aaacatactc aaagccggaa caggctttgt
    10501 atttaatata tcaatagagg gattatgcat aacatttgtt ttctttacaa tagtgtccgc
    10561 attaatatta cttaacaata tccgacaagt acatttaacc aaacctgttg aactgttaaa
    10621 aggaagcagt ttgggagaaa aagaacctaa aacacgatgg ctgcttgcaa taataggagt
    10681 tctttccaca ggggccggtt actatatagc tttatcggta gaaaatccgc ttgcagcttt
    10741 aaatattttc tttttggcgg ttatattggt aataataggc acatactgtc tttttactgc
    10801 aggcagcata gcacttctca aacttctcag gaaaaacaaa aagtactatt acaaaacaaa
    10861 tcattttaca gccatttcgg gcatgattta ccgcatgaaa cgaaatgcgg tagggttggc
    10921 aaatatatgt atactgtcgg caatggttct tgtaaccgta tcctcaaccg tctgtttata
    10981 tgtcggtatg catgatgccc tgaacaacag attcccgaca gatattgaga taatcactca
    11041 caatgcagat aaagaacagt cagtatccat aagcaacgcc gttaacaaaa ttgtttcgga
    11101 aagcggttat gaaatttcaa aatacagcga attcaggtac ttgtcacact caatgctata
    11161 tgaagaagaa aaagacacct tttctatatg tttcgatatc aataaaaact ccgacaatat
    11221 gtattatgta tatttcttcc ctgtcgaaga ttacaataaa aaaacaggca ctgatattct
    11281 ccttgaagat aaagaagtac ttatatacaa tcgcaacaat gttgatttta caggtaattt
    11341 aaatatacaa agcatagagt tcattgttaa aggaaatgta aaagacccgg gttttcccat
    11401 gtccgttgac ggttttaaca aggtttattt attctttaaa gacgaacagg taattataga
    11461 tataaacaaa cagtttgagg agtatttcgg tgcttacagt agccttacac atacaatagc
    11521 ttttgatgtt atgggagata aggcagaaca gttaaagctg accgatagaa taaaggttac
    11581 ctttgaaagg cttgaagaaa acatttccat caatacaata tccaaagcgg aagaagaata
    11641 cgtgttttac gcaatttacg gaggactgtt tttcttaggg ttgtttttag gagtgctttt
    11701 tatgatggca accgtcctca taatctacta caaacagatc tccgaaggtt atgaagacaa
    11761 gcagaggttt catataatgc agcaagtagg catgacaaaa gccgaagtca gacaatccat
    11821 acgctcacaa gtacttacgg tatttttcct gcctttaata atggcaatta tacatattgt
    11881 tttcgcattc agaatgataa caaaaatatt aggagtattg taccttacaa atgtaccgct
    11941 attcataaac tgtaccgtaa nnnnnnnnnn ggcttttaca ctattctaca tggcggcata
    12001 ttcgctaacc gcgcatagtt actacaagat tgtagagtga aaacggaaac aaagtttaga
    12061 cagcattaca aaaacagaat gcttattaaa ccttaataac gtatttatta agataacagg
    12121 gatattttgt aagatcctcc ctgtaaggag ccaacacaac tatattccct gtttctttat
    12181 cctcataaac tccgtgattg gaatagtcca ctacattgga agtttttaca cttaagttgt
    12241 gatgctctct tttagtgtcg ataacggtaa cactcttttt tattatcctt ttagtttcct
    12301 tatcgacttt tgctatgcat aaaggatatc tgggcaggtt tcctttgggg ttttctcggt
    12361 ttattacacc tatgaaatag agattaccgt tactgtgatt gagcaatttg ggtatgcttg
    12421 aagaagagta cattatactg ccgtcatcat acagaagcat ttcgggatct gaccaggtat
    12481 agccgtaatc ttctgatata ctgtagaatt ttgcaccgat tatgctgtcc gactgcgtat
    12541 aattgctgtt acgcataacc attaatattt cattttcttt tccgtctatt attccgaatg
    12601 caggctcata cagtcccctt atcgatttgt ccggctccac agtcacatac tcccctaaat
    12661 cccattcata ttcaagtttt tcactgttcc atgccgcttt taaagccccc gctttcatat
    12721 atcccatacc tgtgggatta aaaagagtac cgtctttatt aagcatctgt acctgtaccg
    12781 gcaccagaag gctgttatct tttgcacgta ctatgagggg cgaaaccgta gctgccatat
    12841 taactccgaa ttccacagat ttcataaagt gttttgtgtt atagcaatcg cctttttgta
    12901 caatgtattt cttatccgtc caggtatatc cgtcatcaaa tgacagtctg taatacaagg
    12961 tcctcattct ccacagggaa gctatcttat tttcctccca gtatgtatca tttgctacat
    13021 ataaaattac ctttgcatcg gtatcacaat acagcgcacc gtagccgctt ttaacccggc
    13081 tgttgttaaa atcatataca taggattttt ctataagccc ctgttctatc caggtgttgc
    13141 cgttatcttt tgaacgtaaa agcacactgc cggcattgct gtccgaagca ttgaacaaat
    13201 cgaaattcat cagtaaatcg tcggaatgga tacaacgtac aaccgacggt cccaatatga
    13261 aatagtccat atattccaga tacttatcgg tcttttcaac aatgtcaaaa aacataacaa
    13321 tctctccgtc ccgctaacat tatatcatct atttatccta ttattatgct ctgattattt
    13381 gtctgattat tttccatatt attttaaatt accatcttgt attttgactg ttgataatat
    13441 actataaatg ctgtcactta tgctttttat ccgccgttct cagatttatc aggtatggca
    13501 gacgggaatc atcagcagat aacatgtggg tttatgtgca gtttcaagtc gttaaattac
    13561 cataaatcaa ttatgttcag tactttaaat aaaccggtat tttacggttt tggaggataa
    13621 acctttaatg gtggaattaa aaaagataac atgggagaac ttcgagcagt gcctgtcact
    13681 gggtgtaacc gacaatcagc aggatttcgt agaagataat gtatatgcac tggcacaggc
    13741 ttatgtagcg gcaaccaatg ataaattccc ccctattgca tacgcaatat ataatgatga
    13801 gactgttgtg ggaatagtca tattggtcca taaatatgct aaagacagca agtattatca
    13861 cgaaaactgt tatgaagtgg taagtttcat gatagggctt cagtatcagg gaaagggtta
    13921 cggaaaagag gcttttgcaa aggtaattga ggttataaag acatttcccg aaggtaaagc
    13981 caaagcaata tgcctgtcat atgatccgca taacgatacg gcaagacact tatatgcttc
    14041 ctacggattt atcgagaccg gcgttataaa cgatttagga gaggtagtgg ctaaattacc
    14101 gatagcaaca tagtttccga tatagttgtc atacaaataa gtccatttat tttattaatc
    14161 atattgattg atataaacct tttatatatt ggtttttacc gggaatttat gatatattct
    14221 acaggacgga aagggatgag ttttaatgag aagaataaaa cttgacacat tgatattcgt
    14281 tgtatttata acggttttcg ggttgctgaa tctgttaaat cccgacaaaa gtgaaatatc
    14341 tttacaggaa aaccgcaccc ttgcaaaaaa accggtattc tctttccaat cagtgtttga
    14401 caagagttat gcatcaggct ggaatgacta ttattccgac accttgctct tgagagaaaa
    14461 tctgatacag gcaaatgtgc aaataaagaa gaacttcgga atagaaaaga atcgtatctc
    14521 catatcggtt ataggaacgg aaccggctcc gacaaagaca cccaaaccta cacagacacc
    14581 tgtggcatcg gtttctcccg ttccgacgga aacgcaagca cataatcccg aaacttccga
    14641 tgagccttcg gcatcaccgt ccccttcacc cgctcccacg cctgtagaag aagaacctac
    14701 cggttccggt gccgcaacag gcatgtatat taccatcggt gatgagacat tcaagctgga
    14761 tacttattcg gaagaagcat atgataacta cgcaaattac ataaataaaa taaagaagat
    14821 ggcgggaccg gatattgaag tatacagcat gcttgcaccg tcccgctcca cctatcttgc
    14881 attaacaaaa tatgatttct taagttcccg tgctgcaaat gtcgaacaga taaacagcaa
    14941 gattgataag tctgtcaaaa atgtaaatgt attgcgctcc atggtcgaac atgttgatga
    15001 atacatatat taccgtacgg atcatcactg gacacacctg ggtgcttatt acggctatac
    15061 ggaacttatg aaagcaagaa acaggcaatc ggaaataatc cctttaagca aatacagtga
    15121 aaaacttgtc gtggaaggtt atctcgggtt ttataataag ccgtacctta cacaggccga
    15181 ttacgataat cccgatacca taatcgcata ctatccgata gtagattaca aatacattta
    15241 caacagtcag gatgtttcaa ttgaaaagcg actcatcaat aaagaaaggg tcaaacccga
    15301 caaagattat tacctgctgt tttccgacgg aggtttcgga acatacagtg aaataaccac
    15361 atcgtccgac tccgatctta agtgtatggt tataaaagac tctttcggag attctttaat
    15421 atccttcctg ttgcctcact acaagaccat atatgtagtg gattcccgca catacaacaa
    15481 ggcattctgc ggtaatatga atatcataga ctttgccaaa tccaaaaaag tggatgaaat
    15541 aatagtcaat tactggatta ccgaaattac atacatggat gattttatga agaaagcata
    15601 tgcaacgctg gagtagttac aaaggtattc cggttgcatt tttcaggagt ttatccctct
    15661 ccttataatc gggcatatac ttctttacaa ggtcgtaaaa aggtttctga tggttccggt
    15721 gccgcaaatg acacagttca tgaactatca cataatccac ggctctttcg ggatacaaga
    15781 ttaatctgta tgaataacat attccgcctt tttgagtgca gcttccgaat cttctttccg
    15841 aggatgttat tttaatatat gcaggataca gccccgttat ttccgaataa tattttgttc
    15901 tttcggtaag tacctgttta gcctgtttct ttaactcctt aatctgttcg tctgtaaaat
    15961 ctttcaccga aggtcggttc ttcttaatct ctatctgcct gtctatccac ttttcatgat
    16021 cggacacaaa tttatcaatg actttttttg aacaaaaaac aggagcccgt actgtaacgg
    16081 tacaatcctg tgagattctt aaagatattg ttcttcttct tgaacgtatt aaaatgtagt
    16141 cgtgcatacg caaaacacaa tggtattaaa caaatatgtt aattccggct ctacttaaag
    16201 aattttatga tagccagaat tacacctacc acactgtaca gccagaccaa atatctgact
    16261 atgctcataa cagtatttac aacgggaata ccgcgtaaga gccattgaat cagtcctaca
    16321 acaaccccta aaaccacata aagcaagatt gctataacca atgtgccgag ttttttcttc
    16381 ttaacatttt tattcagcgg aaatagtgcg tccatatatg tctccttcta tttgtgatac
    16441 ttacacttta tcatacggag ccgttaaaaa caatgcgatt aagtgaacaa aaatatattg
    16501 ccttctcttt aacccgatta tataataaaa cgaaactgct aggagagcaa catgactttt
    16561 tttgaactgc tgctgatagc gataggactt tcaatggatg cttttgcggt tgcaatatgc
    16621 aaaggtcttt cacttaagaa agtaacctgg aaagatgcct tgaccgtagg gctctatttc
    16681 ggtatttttc aggctgctat gcctttggca ggctttctta taggatctgc tttcgcagga
    16741 attattgaca aatgggacca ctgggttgct tttactcttt tggcggtaat aggaattaag
    16801 atgattatag agagcaaaaa gagaggctcc tgccggtctt cggaagacaa cagtcaatct
    16861 ttaagtttta aacaaatgct ccctttggca ataggcacct ccatcgacgc tcttgcggtc
    16921 ggtgtatctt ttgccttatt atccattaaa atccttcccg cagtcagttt tataggagct
    16981 gttacactta tcttatcttt tgccggggta tatgcaggca gtattttcgg gtgcaggttc
    17041 aagtcgggtg cggaaataac cggaggtata atattaatac tgaccggcct taagatactg
    17101 ctcagtcacc tcggaattat acccttttag gctaatcttt ttcacagtat aaataattgc
    17161 agtccaatcg gataacaata caatgcacat taataattgt gcatctgtat tgcggataat
    17221 tccgactcaa aaatcaaaaa atattacatg aaattcataa acaggtcgaa gttcatgccc
    17281 ctgactttat cctgtatggt aaccatgtcg tatatccaca gtataccgca aagaccgccg
    17341 gtcagtaact tcaaaattcc cattccgata tcattcatga ggaatctgtc tacacctaac
    17401 tgcccgataa atatcgagac cagtaagtat gtggtgggat ccttcatctg aacgaacatc
    17461 aaacgctgaa tttgctcgtc ggtcatggaa tccaattttt ctttcacggc atataatttt
    17521 tctgccggaa aaaacttctg attagtcata aaatagatgt ctgcttttgc gctcatgtct
    17581 ctcttgtatg atacagtcag tgactgttca tactcctcct tcttgtatat ttcgct
//
LOCUS       DAOSOM010000036        16012 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_3687_length_16012_cov_6.544025, whole genome shotgun sequence.
ACCESSION   DAOSOM010000036 DAOSOM010000000
VERSION     DAOSOM010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 16012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16012
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3687_length_16012_cov_6.544025"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="PLF14_07970"
     CDS             <1..404
                     /locus_tag="PLF14_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71256.1"
                     /translation="TFADCKVFVMKKGSDKADYLEDILLFLTSKEVQEQSFIECANLP
                     AYKNAAKEFASMQTDNLETELAARQLDMFDYGIPQPFGTPTSQFYYYSKGAPDLIREI
                     LENKDGLFGTTEAIRTQLQKVENIWKTGERE"
     gene            430..1830
                     /locus_tag="PLF14_07975"
     CDS             430..1830
                     /locus_tag="PLF14_07975"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOT71257.1"
                     /translation="MDRKKYGISGFFQRIAQPFINFYKRFMDGSIGTKMSHFIMGAGN
                     IYHGQIIKGLIYFLIQVGYVVFMVTCPKVNNTPLGYKAFINFVTLGTQEGDIFTPADN
                     SMLMLLFGVVTFGITLMYLFAYNSSIKSSYKADKDLKNYGKVTTFRHDLKEMFGNRFY
                     IAMLTPATLGAVVFTLLPTLFMILIAFTNFDKERQAGLKLFDWVGLDNFTTFFTNPSG
                     EIAARFLPVLGWTLIWAFFATFLNYYGGILLALLINKKGLKGKVIFRAIFVLTIAIPQ
                     FISLLAMRNLIGEYGPLQSFLIKVGIMSEPMSFLANAPNVWIARITIILINLWVGIPY
                     SMLLASGAIMNIPESLYEAARIDGASKFTILRKITMPYIVFITTPYLISSFIGNITSF
                     NIIYLLTGGMPKVTGGYIAGGTDLLVTWLYKLTIDEQQYNLGAVIGILTFLLTSTVTL
                     ITYRRSKSYKEEDAFQ"
     gene            1827..2744
                     /locus_tag="PLF14_07980"
     CDS             1827..2744
                     /locus_tag="PLF14_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010772099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOT71258.1"
                     /translation="MSEKNENVKVKKIRPNYLNLSSSEKISRLGAYILLIVLCIIWLY
                     PIVWIVLNAFRTEFNPDGTMVGTVVSHYWPKQVGWDNFKILFTNTYFPVWFRNTLVVA
                     VFTCILSTMLNMSVSYVMSKVKFKMRKPFMNIAMVMGLFPGFMSMIAIYYILKALGLT
                     QTLFALILCYSAGAGLGFYVGKGFFDTVPNALIEAARIDGAGEARIFATIILPISKPI
                     IVYTALMSFTGPWMDFIFARVILGEQNTHLHTVAVGLYYMMYGQIVDTSRFTVFAAGC
                     VCVAIPIVALFMYLQKYYIEGSTAGSVKG"
     gene            2741..4360
                     /locus_tag="PLF14_07985"
     CDS             2741..4360
                     /locus_tag="PLF14_07985"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOT71259.1"
                     /translation="MMKNITKKILCILLSILILLTLVSCSTDALRGEKAIKIEQMPAD
                     NIPGVYYEIFVGGFSDSNKDGTGDIQGIINRIDYLNDGNPLSGKSLGIDGIWLMPIMQ
                     AGSYHKYDTVDYYSIDYNYGTMEDFENLIAVCDEKGIDVIIDLVLNHTSEYNKWFTQL
                     KKAIKEGDKDNKYLDWYSITTDSSKGWHKLATDPDGVDYFYEGNFSPSMPELNMDNKE
                     VLDEIENIVKFWLGKGVKGFRLDAVKYVYLNDDTRNIEFWKWFADTCKKYDKDVFLVA
                     ENWSGNTSIIDYYAALDCFDFGMSGSAGQVALTVNGIFNLNDFVKNICRYKENVEKSN
                     PDAVLKPFISNHDMDRCAGYLPVKEYRMHMAANMYILTSGNPFMYYGEEIGMYGSRAT
                     SSTDANRRLAMLWGDNDTVKDPPGTTFPAERQVNGTVKKQVKRADSLYTHYKKLLALR
                     KANPEIAVGHYTPLYFEGYNLFGGFLVEHNNVVKGVFHNLSDKPITIDLSPYLNAGTS
                     FNSISGYIGQGKASIKKNTLTLDGMTSVILK"
     gene            4506..5090
                     /locus_tag="PLF14_07990"
     CDS             4506..5090
                     /locus_tag="PLF14_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71260.1"
                     /translation="MKKIKVIAIVLTLVLALGSLVACTPDTVLENTEKDYYVTGQFAG
                     WGDAVGKDQFRMTAVSLKDARVAALKAQLKGAKYLYILEHVVITDSGAGWTAQYVENG
                     AVKDCDGNQTMKWLQVAKGQEAPDWWAQSPESGPVTSLTPDLLWIPGFTETPAVGPDW
                     NGNPVVLKAGTYTVVFAMVEKDTGLEKVAGLIAE"
     gene            complement(5157..6083)
                     /locus_tag="PLF14_07995"
     CDS             complement(5157..6083)
                     /locus_tag="PLF14_07995"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HOT71261.1"
                     /translation="MRLTNFIENGKFYKANYHAHSTRSDGQLSQEEAVNVFKEHGYNF
                     LCLSEHNIVTKTDKYNTDDFILVPGVELHSNRDYPLTIHHLLGLTTMDNDKIYDGMRL
                     EDIPFTESKESLLKLEKTLLDLDLLTVYCHPTWSRVEPHQYDFLKDTHIVEICNWGTV
                     YSQNHDNDRVYWEMLLKQGKQIWGMANDDSHRREQYCGGWNMVKCEEFTVESMLDSLR
                     KGSFYATTGPEIYDFYIEDGYGFVKCSEAKTITFITLSRWGKCYRDPAAKLTEASLNL
                     SSSAYMVRVEVEDKYGNIAWSNPIMRMDRFEY"
     gene            6273..7364
                     /locus_tag="PLF14_08000"
     CDS             6273..7364
                     /locus_tag="PLF14_08000"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOT71262.1"
                     /translation="MIKPKTENIKIFRSKDSVFGYHGWPSVCRGLNGELLAVCSGSRL
                     GHVCPFGKVLLSRSYDEGRTWTVPEIIMDTPLDDRDAGITVFNKNKIIITSFNHFIER
                     QSKFPQEGFKLAMRDAYFSNNHITKEMEKKYYGSTYVISDDKGLTWSKVMHAPVTSPH
                     GPTALKNGGLIYMGTYKEADENPAKYDKKLTFEKELLVFYSKDGFKWEYLSHVPYVTE
                     EGYEYYHCEPYILELKDKLVAFIRVQRGLFATLMTQSYDQGKTWSNPVRINKHGSPPH
                     ALLHSSGAVILTYGYREKPFGQRAMISYDNCKTWSEEIILRDDGPDNDLGYPASVELK
                     DNSILTIYYQKETSDSLCEIFGTIWELPK"
     gene            7377..8411
                     /locus_tag="PLF14_08005"
     CDS             7377..8411
                     /locus_tag="PLF14_08005"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007131233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOT71263.1"
                     /translation="MALQKYIVSKDDSIYEAWPDIALTKSGNLICVFSECEHHGNRDN
                     ARIVYCHSLDRGNTWSKKTALTKKGTKEEFFNCARICVMPDDSLIIVADYIFGNENHK
                     AQTFIWKSFDDGITWQGPFDNPSSGIVPDKIRVLKSGRWIISSHFLDREINKLVQYLW
                     YSDDRGKTWSDAVIVAKDERYNLCETCIVEVEDNVLVAFLRENSWEGYDCFKAFSYDG
                     GVTWDGVYRIPIPGCHRPVAGFLQDGTILMTYRFIQGGKQGWGKTTQNFMGCIFDKNT
                     ALAKERRQQQTRIFPIDYDRNPKSDLGYSGWVQFDDGTIYVVTYLLDDSPKGQIRGYS
                     FRMEDFLLPE"
     gene            complement(8494..9381)
                     /locus_tag="PLF14_08010"
     CDS             complement(8494..9381)
                     /locus_tag="PLF14_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005979550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HOT71264.1"
                     /translation="MKNLSISDKSMKIIIKAQKDEETGALLYDYLAKKEKDPENKKLL
                     HRMASDELSHAKTWKSYTKKDIKANRLKLFFVKIVSTILGYTFTLKMMQKDEQFAQRD
                     YEILKVEVPDAIKIQEDEHRHEMELIAMLDEERLQYVGAMVLGLNDALVELTGAIAGL
                     TFALANTKVVALSGIITGISATLSMAASNYLAEKADGNPKALKSSVFTGVAYLITVAL
                     MVLPYLLLPADAYLEAFLTMIAVVILIILVFNYYISVAKDQPFFKNFLKMALISISVA
                     GISYLIGILAKNLLGIDVM"
     gene            complement(9392..10492)
                     /locus_tag="PLF14_08015"
     CDS             complement(9392..10492)
                     /locus_tag="PLF14_08015"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT71265.1"
                     /translation="MSKIIRGKETLTSKERIQRTFAFEPVDRVAMGYDTNPIAHENLC
                     KVLNMDPKEKFAFLRYIGVDHAGAWFKYIGEKIYPDIPDRNRSSEHGAVMKYVKNQYG
                     GYWDYCDFPLLDVDDEVIYNYPFPNPDDFDYEFYKQRVDYLCDMGFAISAGNAGVGDI
                     MNTTGMLMGVEQALINICIDHEATLHMIDKRLNMQLKRLERVLELCKGKHDFMWLGED
                     LGSQHAPLISMDLYRRVLKPRHQKFIDLAKAYDLPVIIHTCGSSSWVYEEFIEMGMNG
                     VDTLQPEAANMSPQYLADHFGGRLVFRGCISTAGPLAYGTVEETKKVCQETLEIMSKH
                     GGYIFAPTHAIQDNTPPENTIAMYNTAHAFRK"
     gene            complement(10489..11382)
                     /locus_tag="PLF14_08020"
     CDS             complement(10489..11382)
                     /locus_tag="PLF14_08020"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOT71266.1"
                     /translation="MKIGTQSAAVDRIFGPEKALSVLKGAGFEAVDFPLDNEVRLNLP
                     YPALKKYFTDIGKYAADSGLEIGQTHAPFYGPDKYMTQYDEVIEIQKRAIAATSFLGS
                     EYVVIHPIMTKERKYNNDYERNKEIILKYYTDLIPTLKEYDVYLAVENMFSWDSVEKC
                     ICPTACTTAEEMAEYIDELGERFVACLDIGHINLIHQKGYEHVNFAHMIKTLGHRIKT
                     LHVHDNDGINDEHLPPFAGNIDWNLVMTCLKGIGYSGNLSLEADNFMTYFRPEQFEKG
                     QKVMYANAKRLYDMFVQGGTK"
     gene            complement(11667..12152)
                     /locus_tag="PLF14_08025"
     CDS             complement(11667..12152)
                     /locus_tag="PLF14_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71267.1"
                     /translation="MDIKRIKIEYMPKITDDQLYDFYVRNDICEAGHGKEAAVKPLKY
                     SPYMVGAFYEGKLVGIVRAVFDGLTAVIMEFCLELELQGDNLQYDNGSIIEKDQYDIA
                     KKMGILLIDELRKIGNTFVSAVILQHSEQDVYTSIGFKFNEGHAEYIIDERPYVLSEI
                     V"
     gene            complement(12170..13516)
                     /locus_tag="PLF14_08030"
     CDS             complement(12170..13516)
                     /locus_tag="PLF14_08030"
                     /inference="COORDINATES: protein motif:HMM:NF013776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT71268.1"
                     /translation="MFIGREKELSTLNQKIDSDKFEFGIVYGRRRIGKTELLKKVVVK
                     HKAIYYVANEMGLDYNFKQISGVVAKYFDEHFTFESLEALFKYLVQKSKTQRIVIIID
                     EFTYLIKTNPEIMSILQNIIDHLLIDSRLKLIISGSHVGMIEDALAYQKPLYGRATFK
                     MKIEAFDYYDAAKFYPKVSNEDKVRLYSIFGGVPFYNSKIDDNRSVEDNIKTLIIEKG
                     AVFEDEVSFFLSQEVRSVATYGKIINAIASGATRLNDISTKSGSGNTGTTSKYLDLLI
                     QLGIVEREYCFGEGANSRKTIYRIKDRLFNFHYSFIEKHKSQRIIMTPDRFYNTVVKN
                     HLDEYVSLEFEQVCKDFLKRKYKETIEEIGRYWYNDRQEKKDIEIDIVMRANNLLSVY
                     ECKWTDSPVGTKVVNELAKKAKSLDYRSLGFFSKAGYDNQEYKADCYIVDDLFSIN"
     gene            complement(13657..15015)
                     /locus_tag="PLF14_08035"
     CDS             complement(13657..15015)
                     /locus_tag="PLF14_08035"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOT71269.1"
                     /translation="MLKNKRIRIIIISTLVALVISALYMLGIISDEIKLYKLEATSVL
                     DNQVSSIRNEFMVNINSVYELRALVYGVQGKYVDPQIIGERILKNPNIRNILYAPDSV
                     VTHVYPLEGNEAVIGLDLLSPENKSYEDAKLAAQSDDVILTGPYELKQGGQAFSARLA
                     VRIPDKNSQLKYIGLVSVTMNFPDLISESTNNLHNSLKYDFILEKRLNDSDEYIEIHS
                     GRVSKYDDYISSDFSVSNMDFRLSVVPQSGWYDKSGIMIKIFAFVVVSLAVGIIMGFI
                     FAARQKLIEKSRTDALTGIYNRAGGTHAINTIIKNNLYKSGAFIMMDLDNFKSVNDKL
                     GHQMGDEVLITTAAILKNSIRATDILARLGGDEFMLYLPFEGDSDFLDKKLEEIRIKM
                     HRDIKGTEGVVTVSSSIGVTFYNKDKNFNTLYEQADKALYQSKESGKNRITIYEAKSD
                     IG"
     gene            complement(15207..15878)
                     /locus_tag="PLF14_08040"
     CDS             complement(15207..15878)
                     /locus_tag="PLF14_08040"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009606555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOT71270.1"
                     /translation="MTKKLFFQAIGKFVFGLVFVALLLFLPAGTLRFWNGWLFIGILF
                     LPISITGIVMMIRNPELLKKRLNAKEKQKEQKTVIFLSGLMFVAAFIVAGLNFRFSWI
                     VLPDWLVYTASGIFVAAYLMYVEVLRENEYLSRTVEVQKNQKVIDTGLYGIVRHPMYF
                     ATLFLFLSMGLVLGSPISFVIMLFYIPIIVKRINNEEKILAQGLEGYEEYRKKVKYRI
                     IPFIW"
BASE COUNT         4739 a         3402 c         3041 g         4830 t
ORIGIN      
        1 gcacctttgc cgactgtaaa gtctttgtta tgaaaaaagg aagtgataaa gccgattatc
       61 tggaagacat attgcttttc ttgacaagca aggaagtaca ggaacagtcc ttcattgaat
      121 gtgccaatct gcccgcatac aaaaatgcag ccaaggaatt tgcctcaatg cagaccgata
      181 atctggaaac cgaattggct gccagacagt tggatatgtt tgattacgga attccccagc
      241 ccttcggtac tcccacatct caattctatt actactccaa aggcgctccc gatcttataa
      301 gggagatatt ggagaacaaa gacggtttat tcggtacaac ggaggctata aggactcagt
      361 tgcagaaagt tgagaatatt tggaaaaccg gagaaagaga ataaaagtcg gtcgaacgga
      421 ggctaaaata tggacaggaa aaaatacggg ataagcggat tttttcaaag aattgcgcaa
      481 cccttcataa atttctataa acgttttatg gatggcagta taggcaccaa aatgtctcac
      541 ttcataatgg gtgcgggaaa catataccac ggtcagataa taaaaggttt aatatacttt
      601 cttattcagg tcggttatgt cgtcttcatg gtaacctgtc ccaaagtcaa caacacgcct
      661 ttgggctata aagcattcat aaacttcgtt acattaggca cacaggaggg agatattttc
      721 actcccgcgg acaattccat gctgatgctg cttttcggag ttgtcacatt cggtattaca
      781 cttatgtacc tgtttgcata caattcatcg ataaaaagca gctacaaagc ggataaagac
      841 cttaaaaact acggaaaggt cacaacattc agacatgact taaaggaaat gttcggcaac
      901 cgtttctaca ttgcaatgct tacaccggca acgctgggcg ctgttgtgtt cacgttactg
      961 ccgaccttat tcatgattct tatagctttt accaattttg ataaggaaag gcaagccggc
     1021 ttaaaacttt tcgactgggt gggactggat aattttacta ctttctttac caatccttcg
     1081 ggagaaattg ctgcgcgatt cctgcctgtt ttaggctgga ctcttatatg ggcgttcttt
     1141 gcgacattcc tcaattatta cggaggaata ttgctggcac tgcttatcaa caaaaagggt
     1201 ttaaaaggca aagttatttt ccgtgctatt ttcgttctta ccattgcgat accgcagttt
     1261 atttcactgc ttgcaatgag aaacctaata ggcgaatacg gtccgctgca gagcttctta
     1321 attaaagtcg gaattatgag cgaacctatg tctttccttg caaacgctcc caatgtgtgg
     1381 attgcaagaa taaccattat acttataaac ttatgggtag gtattcccta ctccatgctt
     1441 ttagcttcag gtgccattat gaatataccc gaaagccttt atgaggcggc aaggatagac
     1501 ggtgcaagca agtttacaat attaagaaaa ataaccatgc cttatatagt gttcatcacc
     1561 acaccttacc ttatatcctc atttataggc aacattacaa gtttcaatat catatatctc
     1621 ctgacaggcg gtatgcccaa agttaccggc ggatatattg cgggaggtac cgatcttctt
     1681 gttacatggc tttacaaact taccattgat gaacaacagt acaatttagg agctgtgata
     1741 ggtattctta ccttcctctt aacatcgacg gttactctga taacctaccg cagatccaaa
     1801 tcctataaag aggaggacgc attccaatga gtgaaaaaaa tgaaaatgta aaggtaaaga
     1861 aaataagacc gaattatttg aatctttcca gcagtgaaaa aatttccaga ttgggcgcat
     1921 atattctcct tatcgttttg tgcataatat ggctttatcc catagtgtgg atagtgctta
     1981 atgcattccg tacggagttc aatcccgacg gaacaatggt aggtaccgtt gtatcccact
     2041 actggcccaa gcaggtgggg tgggataatt tcaagatact ttttacaaac acatattttc
     2101 ccgtatggtt cagaaatact ttggttgtgg ctgtattcac ctgtattctg tccacaatgc
     2161 tcaatatgtc ggtatcttat gttatgagta aggtaaagtt caaaatgaga aaaccgttta
     2221 tgaacatagc catggtaatg ggtttgttcc ccggatttat gtccatgata gctatttact
     2281 acattttgaa agctctggga ctgacacaga cactgtttgc tttgatactc tgctactcgg
     2341 caggtgcggg attgggattt tatgtaggca aaggcttttt cgataccgta cccaatgcgc
     2401 ttattgaagc ggcacgcata gacggcgcag gagaagcacg aatattcgct accatcattc
     2461 tgcccataag taaacccata attgtatata ccgctttaat gagttttacc ggaccgtgga
     2521 tggatttcat atttgcccgt gttatactgg gtgagcagaa cactcatctg cataccgttg
     2581 cagtcgggtt gtattacatg atgtacggac agattgtcga cacatcccgg tttacggttt
     2641 ttgcagcagg ctgtgtatgt gtagccatcc cgatagttgc gttattcatg tacctgcaga
     2701 aatactacat agaaggaagc acggcgggtt cggtaaaagg atgatgaaaa acataacaaa
     2761 aaaaatatta tgcatacttt taagcatctt aatactgctt acccttgttt cttgcagtac
     2821 tgatgcgtta agaggcgaaa aagccataaa aatagagcaa atgccggcgg ataatatccc
     2881 cggcgtttat tatgaaatat tcgtgggagg tttctccgac agcaataaag acggaacggg
     2941 cgatatacag ggaataatta accgtatcga ttacctcaat gacggtaacc ctttaagcgg
     3001 taaaagtctc ggaatagacg gcatctggct tatgccgata atgcaggcag gttcctatca
     3061 taaatatgac accgtagatt attacagtat tgattacaac tacggtacga tggaagattt
     3121 tgagaacctc atagccgttt gtgatgaaaa aggtattgat gtgataatcg atctggttct
     3181 caaccacaca tcggaataca acaaatggtt tacccaactt aaaaaagcaa ttaaagaggg
     3241 agataaagat aataaatatt tagactggta cagtattaca accgattcgt ccaagggatg
     3301 gcataaactt gcaacagacc ccgacggtgt cgactatttt tacgaaggca acttctctcc
     3361 ttccatgccg gagctgaata tggataacaa ggaagtgctt gacgaaattg aaaatatcgt
     3421 taaattctgg ctgggaaaag gcgtaaaggg tttccggctg gatgccgtaa aatatgttta
     3481 tctgaacgat gatacccgca atatcgagtt ctggaaatgg tttgccgata cctgcaaaaa
     3541 gtatgacaag gatgtattct tagttgcgga aaactggtca ggtaatacca gcataatcga
     3601 ttattatgcg gcattggact gctttgattt cggtatgtcg ggctcggccg gacaggtggc
     3661 acttactgta aacggcatat tcaatctgaa cgactttgtc aaaaatatat gccgctacaa
     3721 agaaaatgtg gaaaaatcca accctgatgc tgtactaaaa cctttcattt ccaatcacga
     3781 tatggacaga tgtgcaggat acttaccggt caaagagtac agaatgcata tggccgcaaa
     3841 tatgtatata cttacttcgg gtaatccttt tatgtattac ggagaagaaa taggtatgta
     3901 cggaagcaga gccacctctt caaccgatgc caacaggcgt ctggcaatgc tgtggggaga
     3961 taacgatacc gtaaaagatc ctccggggac aacattcccg gctgaaagac aggtaaacgg
     4021 taccgttaag aaacaggtaa agcgggccga cagtttatac actcattaca agaaactgtt
     4081 agcattaaga aaagcaaatc ccgaaattgc ggtgggacat tatactcctc tgtattttga
     4141 aggttataac cttttcggag gatttcttgt tgagcacaat aatgtagtta aaggagtctt
     4201 ccataacctt tccgataaac ccataaccat tgatttgtca ccgtatttga atgccggtac
     4261 ttcattcaac tctataagcg ggtatatcgg acaaggaaaa gcaagcataa aaaagaatac
     4321 tctgacactg gacggcatga cttcggtcat acttaaatga taaaagttta caaaagattt
     4381 acattgaggg aaaagttcac aaatttttca caatttgcct aaaatccctt gactgtatta
     4441 atcgttgtga tagaatagga ttgttcatta taagtagtac aacatactaa aaacaggagg
     4501 aatttatgaa aaaaatcaaa gttatcgcta ttgttctgac actggttttg gcactcggat
     4561 cacttgtcgc ttgcacacct gataccgttc tggaaaacac agagaaagac tattatgtaa
     4621 caggacagtt cgccggatgg ggcgatgcag taggaaaaga tcagttcagg atgaccgctg
     4681 tttccttaaa ggatgcacgt gttgctgctc tgaaagcaca gcttaaaggc gcaaaatatc
     4741 tctatatcct tgagcacgta gtaatcaccg actccggtgc aggctggaca gctcaatatg
     4801 tagagaacgg cgctgttaag gattgcgacg gaaaccagac aatgaagtgg ttacaggttg
     4861 caaaaggaca ggaagctcct gactggtggg cacagagtcc cgaaagcggc ccggttactt
     4921 ccctgacacc cgatcttctc tggattcccg gatttaccga aaccccggca gtaggaccgg
     4981 actggaacgg aaaccccgta gtactcaaag caggcacata cactgttgta tttgctatgg
     5041 ttgagaaaga taccggattg gaaaaagtag caggccttat agccgaataa gcaaagtaaa
     5101 ctacacaaaa aggcggcttt cgtagccgct ttttttgtgc ctttatttac cgcttactaa
     5161 tactcaaatc tgtccattct cattatggga ttggaccagg ctatattgcc gtatttatcc
     5221 tccacttcca ctctgaccat atatgcgctg ctggacagat ttaatgaagc ttcggtaagt
     5281 ttagcagccg gatctcgata gcattttccc catctgctca gcgtgatgaa tgttatggtc
     5341 ttagcttccg aacatttaac aaatccgtag ccgtcctcga tatagaaatc atatatttcc
     5401 ggtccggtag ttgcataaaa actgcctttt cttaaactgt ccagcataga ctcaacggta
     5461 aattcctcac acttgaccat attccatcct ccgcaatact gttctcttct gtgcgaatcg
     5521 tcgttggcca ttccccatat ctgttttccc tgttttaaca gcatttccca gtacacacgg
     5581 tcattatcat gattctgcga atataccgtt ccccagttgc atatttccac aatatgcgtg
     5641 tcttttaaga aatcatactg atgaggttcc actcggctcc atgtgggatg acaataaacg
     5701 gtcaggaggt ccaaatccag caatgtcttt tccagtttca gcaacgattc cttactctcg
     5761 gtaaaaggta tatcctccag tctcataccg tcataaatct tatcattatc cattgttgta
     5821 agtcccaaca ggtgatggat agtaaggggg taatcacgat tggaatgtaa ttccaccccg
     5881 ggaacaagta taaaatcatc ggtattatac ttatcggtct ttgtcactat attatgctcg
     5941 gatagacata aaaaattgta gccgtgctct ttaaaaacat ttacagcttc ttcctgagac
     6001 agctgtccgt cacttcttgt cgaatgtgcg tggtaattgg ctttatagaa tttgccgttt
     6061 tcaataaaat tggtcagtct catatgtgca acctcatttc tcaacgcata taaatatctg
     6121 cgtttgccta atacacaaga ttatacaaca tgaatatagt ttttagatta ttttttattc
     6181 atattaatgt aaatttatta tttacgaata tacgccttaa tgaacggtaa ttatggtata
     6241 atttttatat cattcaaatt gaggtattta caatgattaa accaaaaacc gaaaatataa
     6301 agatattccg ctcaaaggat tcggtattcg gatatcacgg atggccttcg gtatgcagag
     6361 gcttaaacgg tgaactttta gcggtttgtt cgggtagcag attagggcat gtatgtccgt
     6421 tcggcaaggt acttctttca agaagttatg atgaaggacg cacctggaca gtccccgaga
     6481 ttataatgga cacacctttg gatgacaggg atgcgggtat caccgttttc aacaaaaaca
     6541 agataattat cacatctttc aatcacttta tcgaaaggca aagtaaattc cctcaagaag
     6601 gctttaaact tgccatgaga gatgcatatt tctccaataa tcacatcaca aaagagatgg
     6661 aaaagaaata ttacggctcc acatatgtta tctcggacga taaaggactt acttggagca
     6721 aggtaatgca tgcaccggta acctccccgc acggaccgac cgcattgaaa aacggcggac
     6781 ttatttacat gggtacttat aaagaagccg atgagaatcc cgccaaatat gataaaaaac
     6841 tgacattcga aaaagaactt ttggtttttt acagcaaaga cggttttaaa tgggagtatc
     6901 tttcgcatgt cccctatgtg acagaagaag gatatgagta ttatcattgc gaaccgtata
     6961 ttctggaact gaaggacaaa cttgttgctt tcatcagggt gcaaagaggt ctcttcgcaa
     7021 cccttatgac ccaatcatac gaccaaggca aaacatggtc caatcccgta agaatcaaca
     7081 aacacggctc tcccccgcat gctcttttac atagtagcgg cgcggtcata ttgacatacg
     7141 gttacagaga aaagcctttc ggacaaaggg ctatgatttc ttatgataac tgtaagacct
     7201 ggtccgagga aataattctt cgagatgacg gaccggacaa cgatctggga tatccggctt
     7261 cggtggaatt gaaggacaac agcattttaa cgatatatta tcagaaagag acttccgatt
     7321 ctttatgtga aatattcgga acaatctggg agttaccgaa ataacgggag gacaatatgg
     7381 ctctgcaaaa atacatagta agcaaagacg attcgatata tgaagcatgg ccggatatag
     7441 cattgacaaa aagcggaaat ctgatatgcg tattctccga gtgcgagcac cacggcaaca
     7501 gggataatgc aagaattgta tactgccaca gccttgacag agggaatacc tggtcaaaga
     7561 aaacagcact tacaaaaaaa ggcacaaaag aagagttctt taactgtgca cgcatttgtg
     7621 taatgcccga tgattcattg attattgttg ccgactacat tttcggtaac gaaaaccata
     7681 aagcacagac atttatatgg aaatcgtttg atgacggcat tacatggcaa ggaccttttg
     7741 acaatccctc atcgggaatt gttccggata agataagggt attaaaaagc ggtcgttgga
     7801 taatatcctc gcattttctg gacagagaaa taaataaact ggtgcagtat ctgtggtaca
     7861 gcgatgaccg gggaaaaacc tggtcggatg cggtaattgt tgcaaaagac gaacgataca
     7921 atttatgcga gacctgcatt gttgaagtgg aagataatgt attggttgca ttcttgcggg
     7981 agaactcgtg ggaaggatac gactgtttca aagcattttc ctatgacgga ggagtaacat
     8041 gggacggtgt atacagaatc cccattcccg gctgtcacag accggttgcc ggctttttgc
     8101 aggacggtac catacttatg acatacaggt tcatacaggg cggaaaacag ggctggggca
     8161 aaactaccca gaacttcatg ggatgtatat ttgataagaa taccgctctg gcaaaggaac
     8221 ggcgacagca acagaccagg atattcccca ttgactacga cagaaacccc aaatccgatt
     8281 tgggctattc cggatgggtt cagttcgacg acggtacaat atatgtggtc acttaccttt
     8341 tagacgattc gcctaaggga cagataagag gatattcttt caggatggaa gattttctat
     8401 taccggaata gtttccaaaa caattaatca gttaccgaat tgatgaataa ctgcataaaa
     8461 gattaaataa gcaaatatac taagtttttt atactacatt acatctatgc ccaacaggtt
     8521 tttggcaagt attcctatta aataggatat tcccgctacc gatatgctta taagggccat
     8581 tttaaggaag ttcttaaaaa acggctggtc tttggctacg gaaatgtagt agttgaaaac
     8641 aaggattatc agtattacca ctgctatcat tgtcaggaaa gcttccagat atgcatccgc
     8701 aggcaacagc agataaggca aaaccatcag tgccaccgtt ataaggtaag ccactccggt
     8761 aaatacgctg gatttgagtg ctttaggatt gccgtcagcc ttttccgcaa gatagttgga
     8821 tgccgccatg gacaatgttg ccgatattcc cgttattatt ccggacaatg ctactacctt
     8881 ggtattggca agagcaaagg tcagacccgc aatggctccg gtcagctcca ccagtgcatc
     8941 gttaagacct aagaccattg ctccgacata ctgaagccgt tcttcatcca gcatggcaat
     9001 cagttccatt tcatgcctgt gttcatcttc ctgtatcttt atggcatcgg gaacttcaac
     9061 tttcagtatt tcataatctc tttgtgcaaa ttgctcatct ttttgcatca tcttaagagt
     9121 aaatgtatat cccaatatgg tgcttacaat tttgacaaag aaaagtttca acctgttggc
     9181 tttaatatct ttcttggtat aactcttcca tgtctttgca tgacttaatt catccgaagc
     9241 cattctgtga agtaattttt tattctcggg atccttctct ttttttgcaa gataatcata
     9301 caataaagca cccgtttctt cgtctttctg cgctttaatg attattttca ttgacttatc
     9361 actgattgaa agatttttca tgttattccc tttatttcct aaacgcatgt gccgtattat
     9421 acatagctat tgtgttttcc ggtggcgtat tatcctgtat cgcatgtgtc ggagcgaata
     9481 tatatcctcc gtgtttactc attatttcca gagtctcttg gcatactttt tttgtttctt
     9541 ccaccgttcc gtaagcaagc ggaccggctg tggaaataca acctcgaaac acaagtcttc
     9601 ccccgaaatg atctgcaaga tactgaggac tcatattggc agcttccggc tgcaatgtat
     9661 ccactccgtt catacccatt tctataaatt cttcataaac ccatgaagaa ctgccgcagg
     9721 tgtgtatgat aacgggcaga tcatatgcct tagccaaatc gataaatttc tgatgcctgg
     9781 gttttaatac ccttctgtat aaatccatgg atataagggg ggcatgttgt gagcccaaat
     9841 cctctcccag ccacatgaaa tcatgtttac ctttacaaag ttccaatact ctttccagtc
     9901 ttttcaactg catattcaat cttttgtcta tcatatgcaa tgtggcctca tgatcgatac
     9961 agatatttat caaagcctgt tccacaccca tcaacatacc ggttgtgttc ataatatcgc
    10021 caactccggc atttccggcg gaaatggcaa atcccatatc gcaaagataa tccacccttt
    10081 gcttataaaa ttcatagtca aagtcatcgg gattaggaaa agggtaattg tatattactt
    10141 catcgtctac atccagaagc ggaaaatcgc agtaatccca atatcctccg tattggtttt
    10201 tgacatattt cataaccgca ccgtgttcgg aactgcggtt tctgtcaggt atatcggggt
    10261 atattttctc tcctatgtat ttaaaccatg ccccggcatg gtcgacacct atatatctca
    10321 aaaaagcgaa tttctcttta gggtccatat taagtacttt acataagttc tcatgtgcta
    10381 tagggttggt atcataaccc atagctaccc tgtctaccgg ctcaaaagca aatgttcttt
    10441 gtattctttc tttagaagtc agtgtttctt ttcctctgat tatcttactc attttgtacc
    10501 tccctgaaca aacatatcat acagtctttt tgcattggca tacataacct tctgaccttt
    10561 ttcaaactgc tccggtcgga aatatgtcat aaaattatcc gcttcaagac tcaaattccc
    10621 gctataacct atccccttta agcaagtcat tacaagattc cagtcaatat tccccgcaaa
    10681 aggaggaagg tgctcatcat ttattccgtc attatcgtgt acatgcaatg tttttatcct
    10741 atgtcccaaa gtctttatca tatgagcgaa gttgacatgc tcatatcctt tctgatgtat
    10801 aaggttgatg tgccctatat ccagacaggc cacaaacctc tcccccaact catcgatata
    10861 ttccgccatt tcttccgctg tggtacaagc ggtcggacag atgcatttct ccacggaatc
    10921 ccaggagaac atattctcca ctgcaagata tacatcatat tctttcagtg tgggaatcaa
    10981 atcggtataa tatttgagaa ttatctcttt attacgctca tagtcgttat tatactttcg
    11041 ctctttggtc attatggggt gtatgactac atattcagac cccagaaaac ttgttgcggc
    11101 tatagccctt ttttgaatct cgataacctc gtcatattgt gtcatatact tgtcgggacc
    11161 ataaaaaggt gcatgagtct gacctatctc caaacccgaa tcggcagcat atttccctat
    11221 gtctgtaaaa tacttcttta aagcgggata cggaagatta agccgtacct cattatccag
    11281 cggaaagtcc accgcctcaa aacctgcacc ctttaaaacg gaaagagcct tttccggtcc
    11341 gaatattctg tctactgccg cactttgtgt tcctattttc ataaactacc tcataaactc
    11401 caaccaagca ttaaataata aaaatacaac tctatcttat gatagtaaca tagcattttc
    11461 aagagtattt cacttaacat tcaatttgag aagcattcat aaacagttaa atctgctcct
    11521 gtttcttact catttaaact tttctgcgat tttttaattt tactttccgg gtaatgtgtt
    11581 tttaggttgc ttgtataccg tttcatagct tccgcagata ctatccattg ttttccacac
    11641 ttgcaggcat cggtaaaagc aattaactat acgatttccg acaatacata cggacgctca
    11701 tctataatat attccgcatg cccttcattg aatttaaaac ctatggaagt gtatacatcc
    11761 tgttcggaat gttgcaaaat tactgcggaa acaaaagtat tccctatctt cctcaattcg
    11821 tctatcagta agatacccat tttctttgct atatcgtatt gatctttttc gattatggaa
    11881 ccgttatcat attgcaaatt gtcgccttgc aattccaact ccaaacagaa ctccattata
    11941 acggctgtca aaccgtcgaa taccgctctt actattccca ccagtttccc ctcgtagaaa
    12001 gcacctacca tatacggact atatttcaga ggtttcaccg cagcttcctt accgtgtccg
    12061 gcttcacaaa tatcgtttct cacatagaaa tcatacagct ggtcatcggt tattttaggc
    12121 atatactcta ttttaattct tttaatatcc atgtggttgt cactcctttt taatttatgg
    12181 aaaacagatc gtcaacaata taacagtccg ccttatattc ctggttgtca tagccggctt
    12241 tcgagaaaaa acctaagctc cggtagtcta aacttttagc tttttttgca agctcattca
    12301 cgactttggt tccgacagga ctatctgtcc atttacactc gtagacagat aacaagttgt
    12361 tcgccctcat tacgatatct atctcgatat ctttcttttc ctgtctgtcg ttgtaccagt
    12421 aacgtccgat ttcttcaata gtttctttat atttccgctt caaaaagtct ttacaaacct
    12481 gctcaaattc caatgaaaca tactcgtcca gatggttttt aaccacagtg ttatagaagc
    12541 ggtcgggagt cattattatt ctctgtgact tgtgcttctc aataaaactg tagtgaaaat
    12601 taaacaaacg gtcttttata cgatagattg ttttcctgga atttgccccc tcaccgaaac
    12661 aatattcccg ctctactatg cctaactgta tcaagaggtc cagatacttg gatgttgtac
    12721 cggtattacc ggaaccgctc ttggtagaaa tatcgttaag ccgtgttgca ccggatgcga
    12781 tagcattaat aatcttgccg tatgtagcta cacttctgac ttcctgactt aagaagaaag
    12841 aaacttcatc ttcaaaaaca gctcctttct caatgataag agtcttaata ttatcttcaa
    12901 cacttctatt atcatctatc ttgctgttgt aaaacggaac accgccaaaa atgctgtata
    12961 acctgacctt atcttcattc gagaccttcg gataaaattt tgccgcatca taatagtcaa
    13021 aagcttctat tttcattttg aatgtggccc tgccgtacaa cggcttttga tatgctaaag
    13081 catcttctat cattcccaca tggcttcctg aaataattaa tttcaacctt gaatctatca
    13141 ataaatgatc aatgatattc tgaagaatag acataatctc aggatttgtt tttatcaaat
    13201 atgtaaattc atctattatt attactatcc tttgagtttt gctcttttga acaagatact
    13261 taaataatgc ttccaatgat tcgaaagtaa agtgttcatc aaaatatttc gccactacac
    13321 cgcttatctg tttaaagtta tagtcaagac ccatttcatt ggcaacatag taaattgctt
    13381 tgtgctttac cacaactttc ttaagtaact ccgtcttccc gattcgcctg cgaccgtaaa
    13441 caataccgaa ttcaaattta tctgaatcaa ttttttgatt gagtgtactt aactcttttt
    13501 ctcttccgat aaacatacga accctccttt gctgtatcac gataaacttt atcgtaatac
    13561 agtttatcat aatgcggttt ataatacaag cccaaaaaca taacttaaaa gcatgtaatc
    13621 tatacttata taaagagact aatccccgat aaatcgctat ccgatgtctg attttgcttc
    13681 atatatcgtt atccggtttt tcccgctttc ctttgactga tagagggctt tatcggcttg
    13741 ctcataaagg gtattaaaat ttttgtcttt gttataaaag gttacgccta tactgctgct
    13801 tacggtcacg actccctccg tgcctttgat gtcgcggtgc atcttaatac gaatttcctc
    13861 aagcttttta tctaagaaat cggagtcgcc ctcaaacgga aggtacagca taaactcatc
    13921 tcctcccagt ctggccaata tatccgtggc acggatggaa tttttcagaa ttgcggccgt
    13981 ggttataagt acctcatccc ccatctgatg ccccagtttg tcattgacac tcttgaagtt
    14041 gtccaagtcc atcattataa aggctccgct tttatagaga ttgtttttaa ttatagtatt
    14101 tattgcgtga gttccgcccg ctctgttata tattcccgtc agtgcatcgg ttctggattt
    14161 ttctattagt ttctgcctgg cggcgaagat aaagcccatg attataccta cagcaagaga
    14221 cacgacaaca aaggcaaata ttttaatcat tattcccgac ttatcatacc aaccggattg
    14281 cggtactacg gataatctga agtccatgtt ggatacggaa aagtcggaag atatataatc
    14341 gtcatatttc gaaactctgc ccgaatggat ttcgatatat tcatcactgt cattcagtct
    14401 tttttccagt ataaaatcat attttaaact gttatgcaaa ttatttgtac tttcggatat
    14461 taaatcgggg aaattcatgg ttaccgatac caatccgata tacttcaatt gggaattttt
    14521 atcgggaatc ctgaccgcca gtcttgcgga gaaggcttgt ccgccttgct ttaattcata
    14581 cggtcccgta agaattacat catcagactg tgctgccaat ttagcatcct cataactttt
    14641 gttttcggga cttagcaagt ccaatccgat aacggcttca ttgccttcca aaggataaac
    14701 atgtgttacc acagagtcgg gcgcatacaa aatgttgcgg atattgggat ttttcaatat
    14761 ccgttcacct attatttgcg gatccacata cttgccttgg actccgtaca ccagagctct
    14821 tagttcataa acggaattta tattgaccat aaactcattt cttatggaag agacctgatt
    14881 atccagtacg gaagttgcct ccagtttata gagctttatt tcatcggata ttatgcccag
    14941 catgtacaag gcacttatta ccaatgcaac aagggttgaa atgattatta ttctgattct
    15001 tttgtttttc aacatataaa caatacctct tgaactttaa ataaaactta cgtatgcttt
    15061 tacgcaaaga tgtgtataaa tctaattatt attgtacaac aatgccctga atttttctat
    15121 gtatatgttc atacgattgt tagtgcatag gaaatacatg ttagggggcg ttataacata
    15181 atctatagat taaatctgag tttgtatcac caaatgaacg gaattattct gtattttact
    15241 ttttttctgt actcctcata accttcaagt ccctgtgcca aaatcttttc ctcgttattt
    15301 attctcttga ctattatggg gatatagaac aacatgatta caaatgaaat aggagatccg
    15361 agcaccaatc ccattgaaag aaacagaaac agagttgcaa agtacatagg atgacgaacg
    15421 attccgtaaa gcccagtatc aattactttt tgatttttct gaacctccac ggttctcgac
    15481 agatactcat tctcacgcag cacttcgaca tacataagat aggcagctac gaatataccg
    15541 gatgcagtat ataccaacca atcgggcagc actatccagc tgaaacgaaa attcaatccc
    15601 gcaacaatga aagcggcaac aaacataagt ccgcttagga aaattactgt tttttgttct
    15661 ttttgcttct cttttgcatt cagtcgcttc ttcaacagtt caggattgcg tatcatcatc
    15721 acgattccgg ttatagaaat cggcaggaaa agaataccga taaaaagcca cccgttccaa
    15781 aatcgtaagg ttccggcagg cagaaaaaga agcaatgcca caaaaaccaa cccgaagaca
    15841 aatttaccga ttgcctgaaa gaacagtttt ttcgtcatag gtttacctcc gcaaatccct
    15901 atttatcctg cttttattat aacttatttt actcaagcct caatacattt gaccgttaat
    15961 cgagtagggc agaattaact gccctctcac accaccgtac gtgccgttcg gc
//
LOCUS       DAOSOM010000037        15917 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_3707_length_15917_cov_5.264090, whole genome shotgun sequence.
ACCESSION   DAOSOM010000037 DAOSOM010000000
VERSION     DAOSOM010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 15917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15917
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3707_length_15917_cov_5.264090"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PLF14_08045"
     CDS             complement(<1..346)
                     /locus_tag="PLF14_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71271.1"
                     /translation="MIPKRFLSTFATAMFIILVIAGMISVILINVKQQRVDAVVDNNN
                     PTSITDNTVKENPDWLDRYIETKQQPFNILLFVTDIKGYDTDAIILFNIDSENGKISA
                     MSIPRDTLVKNQQ"
     gene            complement(484..1446)
                     /locus_tag="PLF14_08050"
     CDS             complement(484..1446)
                     /locus_tag="PLF14_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HOT71272.1"
                     /translation="MLKYEDLPDYCRDFLSYLETIQNRSPQTVNEYYYDIRMFLRFVK
                     ILKFNAKEEFDEITVKDMDVSVLEKLTLSDFYAYMTFLSRNRGASSKTRARKVASLRS
                     FYKYLFSKVKLINNNPALELETPKHKKGLPRYLNLEESTKLLDNVESRHEKRDFAMLT
                     LLLNCGIRISELVGINITDYRDDTIKISGKGNKERTIYLNDACRNAINEYMAVRPNDK
                     AVDKNAMFLSERYTRISRKTVHVIVKKQLANAGIDTTKYSAHKLRHTAATLMYKHGDV
                     DIRALQRILGHESIATTEIYTHLDDEQLRSATKKNPLADYKKEK"
     gene            complement(1439..2902)
                     /gene="gltX"
                     /locus_tag="PLF14_08055"
     CDS             complement(1439..2902)
                     /gene="gltX"
                     /locus_tag="PLF14_08055"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOT71273.1"
                     /translation="MSHNEVRTRYAPSPTGFVHIGNLRSALYEYLIAKVKNGKFILRI
                     EDTDQKRYVEGAVDVIYDTLKIVGIKHDEGPDIGGHYGPYVQSERKHLYLPYAKQLIE
                     KGKAYYCFCSEERLEDLRVKAEKAKVQNKYDRHCLNLSKEEIQRRLDAKEPYVIRQLM
                     PDTGETTYNDAVYGDITFNNDVLEDQILIKSDGFPTYNFANVIDDHLMKISHVVRGSE
                     YITSTPKYVLLYEAFGWEPPIFVHLPLVEKLGGGKLSKRSGDAYFSDLIKAGYLPEAV
                     VNFIVLLGWSSGTEQEFYTLSELEKVFDIKGISKSSSVYDTDKLKWMNGEYIRRKTPE
                     EFVKIASPWIDKALPDTLDRTKICSMIQKRVEVLSEIPDMVKFLKKAKPIDISLYNNE
                     KSKCNINEAKRILPIIRDKIVAMEDFNHESIYLMFTSTAQELGVKNGLVMWPARIALS
                     GQLVTAGGAVEIADVLGKQETLKRIDKAIKQLEKTDA"
     gene            3040..4590
                     /locus_tag="PLF14_08060"
     CDS             3040..4590
                     /locus_tag="PLF14_08060"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HOT71274.1"
                     /translation="MTVKILTDNKTYEFDTRTLKHPNIYSLIKSIPEIDFSAPCGGGK
                     RCGKCKIRLLNDNTGISEEERRFLTKEEISDNIRLACFVALSDGQVVDLRETESLQSI
                     MTEDRLSHDNITVNSITDRGYGVAVDIGTTTLAASLYDLKTAKRLSVTSDVNRQGRFG
                     ADVISRIQFASSKENLLLMQDTVLTQVNRMILRLCKQSAINCKDIYVVAVSGNTTMQH
                     IFMGLDPTGIGVAPFTPVTLDTHIFDTDAPELKWDVKINKKGKIVVCASIASYVGADI
                     LVGILATGIHKADKPCILLDIGTNGEIVLGSKDGIFSCATAAGPAFEGANISCGVAGI
                     EGAINTVSYDDANKFTTIGNKKPIGICGSGLIDAVYSMLKNGIIEDSGYMESEEFKIT
                     DNVILTQKDIREVQNAKAAIAAGLKILVKRSGYKYSEIDKLYLAGGFGTVMNVESSTG
                     IGLIPAELKDKVIPAGNTSLAGTSMLLLDKANIETIDSIRKMTQYIELSQDNEFTEEY
                     VDNMFFEV"
     gene            4600..5145
                     /locus_tag="PLF14_08065"
     CDS             4600..5145
                     /locus_tag="PLF14_08065"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOT71275.1"
                     /translation="MQNLTFTDKQSVRKYMIEKRNALTYREVQNLSEEIFKNFLNSDL
                     TMYESYMVYMPIKNETDTYLLIDYLLNTGKQVSVPYIDKKNKLVPVLIEKNTVYKKDT
                     FGINIPEQIQIVSPDLLDVIFVPGVAFDISGNRVGYGRGYYDAFLKDLHCITCAWCYD
                     FQIIDKIPDVSPADVRIKRFL"
     gene            complement(5151..5804)
                     /locus_tag="PLF14_08070"
     CDS             complement(5151..5804)
                     /locus_tag="PLF14_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71276.1"
                     /translation="MRKMTYISSHKRKKRFNYEIFGLLYCLALIGIIIYYHLTKDISI
                     SAFKADLFSPEYHTVLKPFYFVLIFCGLFLLIFSFGFIGIFRTRISYLLYPASVLTCI
                     FLSLSGFLYELHGRFHFFNACLISASVLFSVLILCFIIEKPYSSKYASLTMRIIDIVC
                     GSLFIAYTIWKISGILRDFLLSYEGYFQLIMLSVSVFFVMSNAVYIYLKFETRYSVK"
     gene            complement(5810..7438)
                     /locus_tag="PLF14_08075"
     CDS             complement(5810..7438)
                     /locus_tag="PLF14_08075"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOT71277.1"
                     /translation="MNNEYIKWKSVRLKDNDLNKELIEMNSKPELIEDAFGRNLTFGT
                     GGLRGILGAGTNRMNIYTVRKATFGLASVLSIGSSVAIAYDSRIKSDLFAKEAATVLA
                     NCKMKVYLFDRVSPTPLLSFAVRQLKCDSGIVITASHNPSNYNGYKVYNSKGCQINPE
                     TADLILRHINKTDIFYELPGETDFDTCIDKGLITYIEDSLIEDFIDTVCKQAFYDGSK
                     RIKIVYTPLNGSGYECVSGAMAKRGFKNFITVREQMHPDGRFPTCPYPNPEVREALQL
                     GIRDLINSKSDILIATDPDCDRAGLAVRKDNQAVILNGNEVGILLLDYICMMRKKKNI
                     MPKCPVAIKTIVTTELATKIAEHYGVTVIDTLTGFKFIGDQIAILEEKGREDSFIFGF
                     EESCGYLSGTYVRDKDAVNAAVLIADMADYYKDKDISLLDRLEELYKKFGYLISYLGN
                     YEYPGTAGLSKMRSIMENLRKSTLLTKDYLNETDMLHADVLKFDIKDFGTAIVRPSGT
                     EPKIKIYYFAEGCDMIDAKEKMKKLTVLIERMLN"
     gene            7640..8479
                     /locus_tag="PLF14_08080"
     CDS             7640..8479
                     /locus_tag="PLF14_08080"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOT71278.1"
                     /translation="MRFSLYANVNKDANGLFLAQVCKYIESIGSEVIHSEIVSKGGKT
                     DISKVMHSDVIITLGGDGTLLDIAQKTKSLSIPLVGINIGSLGFLTQIEKNNYEELIK
                     SITLKQYTISKRAMLSAKVYRNDKLIYDIDALNDVVITRKVTGDTVAVSVDINGSYID
                     TYDGDGVIISTPTGSTGYQMSAGGPVSDPEADIMLMTPICPHMMHKQTYILKDDSNIV
                     LTVEEDESFDCCFSYDGIASSLKGRDVIKVKKSNSFIPLLKIQGNSFYENLRNKIYYR
                     GAK"
     gene            8476..8925
                     /locus_tag="PLF14_08085"
     CDS             8476..8925
                     /locus_tag="PLF14_08085"
                     /inference="COORDINATES: protein motif:HMM:NF014864.2"
                     /note="regulates arginine biosynthesis when complexed with
                     arginine by binding at site that overlap the promotors of
                     the arginine biosynthesis genes; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HOT71279.1"
                     /translation="MKNKRQVKILEFIKQYPVQTQEELVTLLQNNGFVATQATVSRDI
                     KELKLFKAINKDGLSVYTSNSDVSDNSHEKLIKVLKASFLSINIAQNIAVIKTLPATA
                     PAVASAIDNLDAKNLIGTIAGDDTIFACFSANEDAQAFYENILDELR"
     gene            8931..10748
                     /gene="recN"
                     /locus_tag="PLF14_08090"
     CDS             8931..10748
                     /gene="recN"
                     /locus_tag="PLF14_08090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOT71280.1"
                     /translation="MLLQLSIRNFAIFKDTTIDFSDGFNVITGESGAGKSVFITALSL
                     INGERGFKEYIRKGCDFSSVEAVYRLKKDLSETISNKFDIDTDDNMLVITRTIYTDSN
                     SVCKVNGKIRNLTFLKDIAEYLCDIHGQYENQTLLNPLSHQRILDSLYQSFIKTSHDE
                     YIALLDEYNTLVDFILKNSGSEQQRAVRRDILAYQIDEIVKSKVEQFDIEELEKQSRI
                     LENSEKYITSLRESINLIEENDSIEKIYLSAHLLNKLKDMDELLAVSQSMMNCYYILK
                     DNFSQLKDISDQFYFDEETYDGIIKKLELFKKLSYKYGTTVEEITAFRQDAEKQLHDI
                     DNFDNIYKEKIQRIEKLSELLYEKGELLNGLRVKAAEHFSKSVETNLHSLEMPDAVFN
                     VHFEKNLKKNTLGYYDFNRKGLYDIEFLISVNKGIDPLPMAKIASGGEISRIMLAIKC
                     IISNNDDIETYIFDEIDSGVSGEAAIMTANKLYELSASKQVISITHLPQIAAKADHHY
                     LISKYSDEDSTYADIKKLTHNERINQLAILFDGKKVSQEGLEYAKNLLDKMKESDSIR
                     VALREVQEGFIGEAERLGLKTEADVVETVKQFRKEKREK"
     gene            10807..10880
                     /locus_tag="PLF14_08095"
     tRNA            10807..10880
                     /locus_tag="PLF14_08095"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10839..10841,aa:Gly,seq:tcc)
     gene            10889..10965
                     /locus_tag="PLF14_08100"
     tRNA            10889..10965
                     /locus_tag="PLF14_08100"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10923..10925,aa:Arg,seq:tct)
     gene            11092..12750
                     /locus_tag="PLF14_08105"
     CDS             11092..12750
                     /locus_tag="PLF14_08105"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT71281.1"
                     /translation="MKLTFYGAAREVTGSNFILDAAGKRIMIDCGLFQGSSATKAKNF
                     DGFKYDVQSVDYVLLTHAHIDHSGRIPLLYKKGYRGTVICTKATKDLCDIMLVDSANI
                     QESDTQYKNNKRFRAGLLPLEPLYTIEDANLALKHFIAVQYYDLIKLDDNITVKFSDA
                     GHMLGSASITIFVTEEGKTTTVSFTSDLGKPDTPIINNPEVLQECDYLVMESTYGNKK
                     HILKEHPEERMFEIIEDTISNGGTVIIPSFSVGRTQEMIYAFNENIALKKKFSIFNKI
                     PVYVDSPLSTLATEVFKKNTQYFDDEAKQYILKGDNPLDFPNLIFTKSVEESKRLNFD
                     NSPKIIISSSGMCDAGRIRHHLKHNLYKEKTTVLFIGYQANGSLGRLLVDGAKSVKLL
                     GETIEVNARIEMIDGFSGHADMDDLDKYISQIKVKPEKIFLVHGEEDQLYPFAERINN
                     TFGIETVIPENYYTYELKKGEKVYELDHDRVLTENTYKKLTLLDLVTDVKRQVKDVSE
                     LIAYDEINNLDDSQITVLIDLYAAMRQNIKDLILGVESNTKVER"
     gene            12740..13900
                     /locus_tag="PLF14_08110"
     CDS             12740..13900
                     /locus_tag="PLF14_08110"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOT71282.1"
                     /translation="MNDKIYYFDNAASTYMYDEVIEYISEINKNLYANASSTHFAGIA
                     CHDIVKENKEALCDILHINPNTLVFTSGGTESNNLAIKGTAFANFSRKGNIVITAYEH
                     ASVINSAKYLEKFGFEIRTCPLKADGTADIDGLYRLIDDKTILVSTAHVSSELGTISD
                     IEKISSIIKAVNPDCLYHVDAVQSFGKIRINLAKLNGVDMMSFSSHKIHGPKGIGALY
                     VKNNVNIDPLFNGSVQSGLRAGTQNISGICGFAQAAKIIHNRIDDNYNRVSGLKRYFI
                     ENLALTGLETRINTPVCSSPYILNIAFKDIKSEVLLNALSERGICVSSGSACSSKSAK
                     ISEVLKAMSLPIDYIEGAIRLSFCEKNTKEDIDYLLKTLEELVPVLSKFIRK"
     gene            13902..15086
                     /gene="thiI"
                     /locus_tag="PLF14_08115"
     CDS             13902..15086
                     /gene="thiI"
                     /locus_tag="PLF14_08115"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003508145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HOT71283.1"
                     /translation="MKIQAIKTLLIKPGEMMLKGDNRHLFEKQLKDNIQSVLDEVCTY
                     NITTESGRYYVYINKSDYTEQEIASMLCKVFGIVGISVAYRIEQDEQLLYEFLSQFSK
                     QIYEKSPFSTFRVTTKRADKDFPTQSTEVSSKAGAVILSVLPDIKVDLFHPGINFNIE
                     IRKKHIYVYHEEYKAHGGIPVGTSGKALLMLSGGIDSPIAGYMMAKRGLQLEAVYFHS
                     APYTSEKAKEKVIDLAKIIKPYTQLNRLHVVHFTKPQMEIYEKCPHNELTIIMRRVMM
                     YVANEIARMSNCKGIITGESLAQVASQTIESLTVTDHAADFLVLRPLIGMDKAEIVDM
                     ARKIGTFETSIEPYEDCCTIFVAKHPKTRPVLDRILQSEKVLDIDKIVEHCILNLEVV
                     RI"
     gene            15083..15781
                     /gene="lepB"
                     /locus_tag="PLF14_08120"
     CDS             15083..15781
                     /gene="lepB"
                     /locus_tag="PLF14_08120"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOT71284.1"
                     /translation="MKEFDKEDNRKNFEEFIQNDDYTLERLCHKVGRRKIIKHRRTEL
                     LLWLRSFIIVIIAVTVIRGFIGEPVKVFGPSMKDTLITGDILVMSKIKLNTAGIERND
                     IVVIEIQPVQFKYLKFLNDIKWFRRMFPSEDRADYIKRVIALEGEEIDFINGIVYIDG
                     KRLDEPYLKEYASTYEVAIDMPYTVPKGHVFVLGDNRALSKDSRIMGAIPIDSIIGIV
                     VFRTWPLDKFGVID"
BASE COUNT         5144 a         2839 c         3134 g         4800 t
ORIGIN      
        1 tctgctgatt ctttacaagt gtgtctctcg gtatactcat agcagatatt ttgccgtttt
       61 ccgaatctat gttgaacagt attatggcat cggtatcata tccttttata tcggtaacaa
      121 aaagcaaaat attgaaaggc tgttgttttg tttcaatata tctgtccagc cagtcgggat
      181 tttctttgac tgtgttatcg gtaattgatg tcgggttatt attgtccaca acggcatcca
      241 ctctctgttg ttttacgttg ataagaatga cggaaatcat tcctgctatt actaatataa
      301 taaacatcgc agttgcgaat gttgaaagaa atcttttcgg tatcatgttg cctccttgtc
      361 cagttacaac ggtatgtatt ttacattaat atccttatgt tgtaaagtag tgtaaaattc
      421 aatactccac cgctatacta tacaaattaa acgttaatct tcacactaag gttcaaaacc
      481 ctgttatttt tcctttttgt aatccgccag aggattcttc tttgtggcac ttcttaactg
      541 ctcatcgtca agatgggtat aaatttccgt tgtcgctata ctttcatgac ccaatatacg
      601 ctgcagagcc cttatatcca catcaccgtg tttatacatc aaagtggctg cggtgtgtct
      661 cagtttgtgt gccgaatact tggtagtatc tataccggca ttggccagtt gtttttttac
      721 tattacatga acggtttttc tgcttattct cgtataacgt tcgcttaaaa acattgcatt
      781 cttgtcaacg gctttatcat tgggacgtac ggccatatac tcatttatgg catttctgca
      841 agcatcgttg agatatatag ttctttcttt atttccttta ccggaaattt taattgtatc
      901 atccctgtaa tcggttatat ttatccctac aagttccgat attcttatac cgcagttaag
      961 taaaagagtt aacatggcaa aatctctttt ctcatgacgg ctttccacat tgtctaaaag
     1021 tttggtgctc tcttccaggt tgagatagcg gggaagaccc ttcttatgtt tcggggtttc
     1081 cagttccaat gcgggattgt tgtttataag ttttaccttg gaaaaaaggt atttataaaa
     1141 ggacctgagt gaagctactt ttcttgctcg ggttttagag gaagcacctc tgtttcttga
     1201 aagaaaagtc atatacgcat aaaagtccga tagtgtgagt ttttctaaaa cacttacatc
     1261 catgtcttta acggtaatct cgtcaaattc ttcttttgca ttgaatttca gtattttaac
     1321 aaaccgaaga aacattctga tatcgtaata atattcattt acagtctgcg gtgacctgtt
     1381 ttgtattgtt tcaagatatg ataaaaaatc cctgcaataa tcgggaagat cctcatattt
     1441 aagcatctgt cttctccaat tgcttaattg ctttatctat tcttttaagc gtttcctgtt
     1501 tacccagcac atcggctatt tccacggctc ctcctgcggt tacaagctga ccgcttaaag
     1561 caattcttgc aggccacatc accagtccgt ttttaacacc caactcctgt gctgtggagg
     1621 taaacattaa ataaattgat tcatgattga aatcttccat agcaactatt ttatccctga
     1681 tgataggaag aatccttttt gcttcattta tattacattt acttttttca ttgttgtaaa
     1741 gacttatatc gataggtttt gccttcttta aaaacttaac catatcggga atctcactta
     1801 atacttccac acgtttttga atcatggaac aaattttggt tctgtctaaa gtatcgggta
     1861 atgctttatc tatccacggt gaagcaatct taacaaactc ttcaggagtc ttcctcctta
     1921 tatattcacc gttcatccat tttaatttat cggtgtcgta cacagaggaa gacttgctaa
     1981 tacctttaat atcaaatacc ttttccagtt ccgacaatgt gtagaattcc tgttccgtcc
     2041 cgctgctcca gcccaaaagc acaatgaaat tgacaaccgc ttcaggcaga tatcccgctt
     2101 ttataaggtc ggaaaagtat gcatcaccgc tgcgcttgga cagttttccg ccgcccagct
     2161 tttccaccag gggaagatgc acgaatatgg gaggctccca tccgaaagcc tcgtagagaa
     2221 gtacatactt gggggttgac gtaatatatt cactgcctct taccacatgc gatatcttca
     2281 tcagatgatc atctattaca ttggcgaaat tatatgtggg aaagccgtcg gatttgataa
     2341 gtatctgatc ttccagtaca tcattgttaa aagtaatatc tccgtatacc gcatcgttgt
     2401 atgtggtttc acctgtatcg ggcattaact gacgtatgac atacggctct tttgcatcca
     2461 gtcttctttg aatttcctct tttgaaaggt taagacaatg tctgtcatat ttattctgca
     2521 ccttggcttt ttccgcttta actcttaaat cttccagtcg ctcttcggag cagaagcagt
     2581 aataggcttt gcctttttca ataagttgtt ttgcataggg aagataaaga tgctttcttt
     2641 ccgattgtac ataaggaccg taatggcctc cgatgtcggg accttcgtca tgcttaatac
     2701 ctacaatttt aagcgtatcg tatattacat ccactgcacc ttcgacatat cgcttctggt
     2761 cggtgtcttc tattctgagt atgaatttgc cgtttttgac cttggcaata aggtactcgt
     2821 aaagagccga acgtaaatta cctatatgaa caaatccggt gggtgacggt gcatatcttg
     2881 ttcttacttc attgtgactc atttaaaaac tccttgatta tttcttctcc ataattatat
     2941 cattatgtcg gatttataca ataaattacc tgtcatattt cccgtatgca tgtataatag
     3001 aggacagaag attatttatc agtaccacag gagatactta tgaccgtaaa aatactaacc
     3061 gataacaaaa catatgaatt cgatactcgg actcttaaac atccgaacat atactcgctt
     3121 ataaaaagca ttccggaaat tgacttttcc gctccctgcg gaggagggaa gagatgcggg
     3181 aagtgtaaaa taaggcttct taatgataat acgggtatat ccgaggaaga aaggcgtttt
     3241 ttaacaaaag aagagatatc cgataatatc cgtttagcat gttttgttgc gctttccgac
     3301 ggacaggtgg tggatctgag agaaaccgaa tcattacaat ccatcatgac cgaagacaga
     3361 ctgtcacatg ataatattac cgtcaattcc ataaccgata ggggatacgg tgttgccgta
     3421 gacatcggta ccacaacatt ggccgcttct ttatacgacc tcaagaccgc caagagatta
     3481 tccgttactt cggacgtaaa cagacaggga agattcggtg cggatgtaat atcgcgtatt
     3541 cagttcgctt cgtcaaaaga gaatcttttg cttatgcagg ataccgttct tactcaggtc
     3601 aaccgtatga ttttaagatt gtgtaagcag tccgcaataa actgtaaaga tatttatgtt
     3661 gtagcggttt ccggcaatac cacaatgcag catattttca tgggattgga tccgacaggt
     3721 atcggagttg caccgtttac tccggtcact ctggatactc atatattcga taccgatgct
     3781 cccgaactca agtgggatgt aaagataaat aaaaagggaa agattgttgt ttgtgcaagt
     3841 attgcaagtt atgtgggtgc ggatattctg gtaggtatac tggcaacagg catacataaa
     3901 gcggataaac cgtgtatatt actggatata ggcacaaacg gtgagatagt attaggttca
     3961 aaggacggaa tattcagctg tgccacggct gcaggtccgg catttgaagg agctaatatt
     4021 tcctgcggtg ttgcgggtat tgagggtgca atcaataccg taagctatga tgatgcaaac
     4081 aaattcacta cgataggtaa taaaaagcct atcggcatat gcggttcggg actaattgac
     4141 gccgtatatt cgatgcttaa aaacggtata atcgaggact cgggctatat ggagtcagag
     4201 gaattcaaga ttaccgacaa tgtaatactg acacaaaaag atatcaggga agtgcagaat
     4261 gcaaaggccg ccatcgctgc gggattaaag atacttgtaa aaagatcagg atataaatat
     4321 tcagaaatag ataaattata tcttgccgga ggtttcggaa ccgttatgaa tgtggaaagt
     4381 tccacgggaa taggtcttat tcctgccgaa cttaaagata aagtaatacc tgcgggtaac
     4441 acatcattag ccggtacatc catgcttctt ctggataagg caaatatcga aacaatagat
     4501 tccatacgta aaatgacaca atacatagaa ctcagtcagg ataacgagtt taccgaggag
     4561 tatgtggata acatgttttt tgaggtttga gaacataaat tgcagaactt aacttttaca
     4621 gacaaacaat ccgtaaggaa atatatgata gaaaagagaa atgctttaac ataccgggaa
     4681 gtacagaatc tttccgaaga aatttttaaa aactttctta attccgattt aaccatgtat
     4741 gaaagttata tggtatatat gcccataaaa aatgagacag acacatattt gcttattgat
     4801 tatcttttaa ataccggtaa acaggtatcg gttccttata ttgataagaa aaataaactg
     4861 gttcccgtat tgattgagaa aaatacggtt tataaaaaag atactttcgg tatcaatata
     4921 cccgaacaaa ttcagattgt atctcccgat cttttggatg tcatatttgt tcccggtgta
     4981 gcttttgata tttcaggtaa cagagtaggg tacggcagag gatattatga cgccttttta
     5041 aaagaccttc actgtataac ctgtgcctgg tgctacgatt ttcagattat agataaaata
     5101 cccgatgtct caccggctga tgtcagaata aaaaggtttt tataagcctc ctacttgacg
     5161 gaatatcgtg tctcaaactt aagatatatg taaacagcat tgctcatgac aaaaaaaacg
     5221 gaaaccgata acataataag ttgaaaatat ccttcatatg acaacagaaa atctcttagt
     5281 atacctgata ttttccaaat cgtatatgca ataaaaagcg aaccgcagac aatatctatt
     5341 attctcatgg ttaaacttgc gtattttgat gaatagggct tttctattat gaaacacaat
     5401 ataagtacgg agaataagac ggaagccgaa ataagacaag cgttgaaaaa atggaatctg
     5461 ccatgcagtt catacaaaaa accgcttaat gacagaaaaa tgcaggttaa aacagatgcg
     5521 ggataaagta agtaagagat gcgtgtcctg aaaataccta taaagccgaa gctgaatata
     5581 agaaggaaaa gaccgcaaaa gataagaacg aaataaaaag gcttcagaac agtatgatac
     5641 tcaggggaaa acagatcggc tttaaatgcg gatatagata tatcttttgt cagatgataa
     5701 tagataatga tacctataag cgccaaacaa taaagtaatc cgaatatctc gtaattaaat
     5761 cgctttttcc gtttatgcga actgatgtat gtcattttac gcatagatac taattcaaca
     5821 tcctttcaat cagtacagtc agttttttca ttttttcttt cgcatctatc atatcacagc
     5881 cttctgcgaa ataatatatc tttatcttag gttcggtgcc ggaggggcgt acgatagcag
     5941 ttccaaaatc ttttatatca aacttaagta catctgcatg aagcatatcg gtttcattga
     6001 gataatcttt tgtcaacaaa gtacttttcc gtaagttttc cattatactt ctcatcttgc
     6061 tcaatcccgc cgttcccgga tattcgtaat tgcccagata ggaaatgaga tatccgaact
     6121 ttttatatag ctcctccagt ctgtcaagaa gggaaatatc cttatcttta tagtaatccg
     6181 ccatatcggc tatcagaact gcggcattaa cggcatcttt atcccggaca taagttcccg
     6241 aaagataccc gcagctttct tcaaatccga aaataaagct gtcttcccga cctttttctt
     6301 caagaattgc tatctgatcc ccgatgaatt taaatcccgt aagagtatca ataaccgtta
     6361 ctccgtaatg ttcggctatt ttggttgcca gttcggttgt tactatggtt ttaattgcaa
     6421 cagggcattt tggcataata ttcttctttt ttctcatcat gcatatatag tctaatagaa
     6481 gaattcccac ttcatttccg tttaaaatta ccgcttggtt atctttccta acagccagac
     6541 cggctctgtc acagtcggga tcggtagcta ttaagatatc cgacttactg ttaatcaaat
     6601 cccttatgcc caattgcaat gcttctctca cttcgggatt gggataggga caggtgggaa
     6661 atctgccgtc cggatgcatc tgttcccgca ccgttataaa gttcttaaag cctctttttg
     6721 ccattgcacc cgatacacat tcatatccgc ttccgtttaa cggggtatac actattttga
     6781 ttcttttact gccgtcataa aatgcctgtt tacacaccgt gtcaataaaa tcttcaatta
     6841 aactgtcttc tatgtaggtt ataagtcctt tatcaatgca tgtatcaaaa tccgtctcac
     6901 cgggtaattc ataaaatata tcggttttat ttatatgccg caaaattaaa tcggccgttt
     6961 ccggatttat ctgacagcct ttggagttat ataccttata gccgttgtaa ttcgacggat
     7021 tatgagatgc cgttataaca ataccggaat cacatttaag ttgtcttacc gcaaaggaca
     7081 ataaaggggt aggggatact ctgtcaaaaa gatatacttt cattttacag ttggccaata
     7141 ccgttgcggc ttctttggca aacaaatccg actttattct ggaatcgtaa gctattgcaa
     7201 ccgaagaacc gattgaaagg accgaagcca atccgaatgt agctttccta acagtgtata
     7261 tattcattct gttggtgcct gcacccaaaa ttccgcgtaa tccgcccgtg ccgaaggtaa
     7321 gattcctgcc gaatgcatcc tcaatcaatt caggcttact gttcatttca ataagctctt
     7381 tattcaaatc gttatctttt aatcttactg atttccactt tatgtattcg ttattcatag
     7441 caaaccctct atattcatcg acatactgat tatacagtaa tatggatatt ttacctatag
     7501 aaaagtcgga aatatatgtt tgtacattgt ttttaactga ttaatgtatt attatacata
     7561 ttgtaaggtg ctttaataat ttaatcggtt aaactcattt aaagagtagc agaagtttat
     7621 ataaaataag gcaggtaata tgagattttc actatatgca aatgttaata aagatgcaaa
     7681 cggactgttt ttagcacagg tctgcaaata tattgaatct ataggttccg aagttataca
     7741 cagtgagatt gtttccaaag gcggtaagac ggacatttca aaagttatgc attccgatgt
     7801 tatcattact ttaggcggag acggtacact tctggatata gcacagaaaa ccaagagttt
     7861 atcaatacct ctggtaggta ttaatatagg gtcactgggc tttttaactc agatagagaa
     7921 aaataactat gaggaactta taaaaagcat aactttgaaa caatatacca tatcaaaaag
     7981 agcgatgctt tcggcaaaag tctatcgtaa tgacaaattg atttatgaca tagatgcttt
     8041 aaatgatgtg gtcataacca gaaaagtaac cggggatacc gttgccgtat cggtggatat
     8101 aaacggatca tatatagata cttacgacgg ggacggcgtt ataataagta cacccacagg
     8161 ctccacagga tatcagatga gcgcaggagg tccggtaagc gatcccgagg ccgatataat
     8221 gcttatgact cctatctgtc ctcatatgat gcacaagcag acatacatac ttaaagatga
     8281 cagcaacatt gttttaacgg tggaagagga tgaaagtttt gactgttgtt tcagttacga
     8341 cggtattgct tcctcactga aaggtaggga tgtgataaaa gttaagaaaa gtaattcttt
     8401 tattccgctg cttaaaatac agggcaattc attttatgaa aatctcagaa acaaaatata
     8461 ttacagaggt gcaaaatgaa aaacaaacga caggtaaaaa tactggagtt cattaaacag
     8521 tatcctgttc agactcagga agagctggtc acattgttgc aaaataacgg ttttgttgcg
     8581 actcaggcta ccgtttcgag agatatcaag gaattgaaac ttttcaaagc aatcaataaa
     8641 gacggtctct ctgtttatac ttccaactcc gatgtttcgg ataattcaca tgagaagctt
     8701 ataaaagtgc ttaaagcatc gtttttgtct atcaatattg ctcagaatat agcggtaata
     8761 aagactttac ccgcaaccgc accggctgtt gcatcggcaa ttgacaatct tgatgctaag
     8821 aatcttatag gcactattgc cggagacgac acaatatttg catgtttttc cgcaaatgaa
     8881 gatgcacagg ctttttatga gaatattctc gatgagttga ggtaatcgat ttgttattgc
     8941 aattgtctat cagaaatttc gctattttta aggataccac cattgatttc tctgacgggt
     9001 ttaatgtaat tacaggtgaa tcgggagccg gtaaaagcgt ttttataacc gctctttctt
     9061 taattaacgg agaaaggggt tttaaggaat atatacgaaa aggatgcgat ttctcctctg
     9121 ttgaggcggt ttataggctt aagaaagact tatcggaaac tatttcaaat aaattcgata
     9181 tagatacaga tgataatatg cttgtaatta caaggacaat ttataccgat tccaactcgg
     9241 tttgtaaagt aaacggaaaa atccgcaatt tgaccttttt aaaagacatt gcggagtatt
     9301 tatgcgatat tcacggtcag tatgaaaacc agactcttct aaatccgttg tctcatcaaa
     9361 gaattttaga cagtttatac caatcattca taaaaacctc acatgatgaa tacatagcac
     9421 tgcttgatga atataacaca cttgtggatt ttatacttaa aaacagcggt tcggaacagc
     9481 aacgtgcggt aagaagagat atactggctt atcagataga tgaaattgtt aaatcaaagg
     9541 ttgagcaatt tgatatagag gaattggaga agcagagtag gatactggaa aattcagaga
     9601 aatatataac ttctttacgg gaaagcatta atcttattga agaaaatgac agtatcgaga
     9661 agatttatct gtctgctcat ttgttgaata agctgaagga tatggatgaa ttacttgcgg
     9721 tttcacagag tatgatgaat tgctattaca ttctgaaaga taacttctcg caactcaaag
     9781 atatatccga ccaattctat tttgatgaag agacatatga cggaattata aagaagctgg
     9841 aattattcaa aaaactgtca tacaaatacg gaactaccgt ggaagaaata actgcattca
     9901 gacaagatgc ggagaagcaa ctgcatgata ttgataattt cgacaatata tacaaagaaa
     9961 aaatacagag aatagagaaa ctttccgaat tactctatga aaaaggggag ttgctaaacg
    10021 gattaagagt aaaagcggct gagcatttct cgaaatcagt ggaaacaaat cttcattctt
    10081 tagagatgcc cgatgccgta tttaatgtac attttgagaa gaatcttaag aaaaacactc
    10141 tcggatatta tgattttaac agaaaaggac tttatgatat tgagttttta atatcggtta
    10201 ataaaggaat tgatccgctt cccatggcga aaatcgcatc gggaggtgag atttccagaa
    10261 ttatgctggc tatcaagtgt attatcagca acaatgatga tattgaaact tatatttttg
    10321 acgaaataga ttccggtgtg agcggtgaag cggctatcat gacggcaaat aaactttatg
    10381 aattatcagc ttccaaacag gttatttcca taactcattt accgcagatt gcagcaaaag
    10441 cggatcatca ctatctgata agtaaataca gtgatgaaga ttctacatat gccgatataa
    10501 agaaacttac ccacaatgaa cgtattaatc aactggcaat actttttgac ggcaaaaagg
    10561 tcagtcaaga aggtctggag tatgctaaaa atcttctgga taaaatgaaa gaatcagata
    10621 gtatcagggt tgcattgcgt gaagttcagg aaggctttat cggtgaagct gaaaggcttg
    10681 ggctgaaaac agaagccgat gtggtagaaa cggtaaagca attcaggaaa gaaaaaaggg
    10741 agaaataaca attaaaaaat ctgattgaca cttatacttc atgtgttata atacataagc
    10801 aatcctgcgg gtgtagttca gtggtagaac actagccttc caagctggtt gtgagggttc
    10861 gattcccttc acccgctcca ttttttatga gccagtagct cagctggata gagcaacgga
    10921 cttctaatcc gtaggtcggg ggttcgaatc ccttctggct cgccacacaa ttcctgtaac
    10981 aggggatttt tttattgtta aataattagt aaaacttata tgttaattca tcagttttac
    11041 gttataatat taaatttatg tgttatcata tacaacaaga ggttttttat tatgaaatta
    11101 acattttacg gggcagcaag agaagtaacg ggatcaaatt ttatactgga cgctgcaggg
    11161 aagagaataa tgatagattg cggattattc cagggatcct ccgcaaccaa ggcaaaaaac
    11221 tttgacggat tcaaatatga tgtacagtct gttgattatg ttcttttgac tcatgcacat
    11281 atcgatcact cgggaagaat acctttattg tataaaaaag gatacagagg aacggttatc
    11341 tgtacaaaag caactaagga tttatgtgat atcatgcttg tggattccgc aaatatacag
    11401 gagtcggata cgcagtataa aaacaataaa agattcagag ccggtttgct tcctctggaa
    11461 cctttataca ccatagaaga tgcaaattta gctttaaaac attttattgc cgtgcagtat
    11521 tacgacctga taaagcttga tgataacatt acggtaaaat tcagtgacgc cggacacatg
    11581 ttgggttccg catctataac aatcttcgta acggaagaag gcaagaccac tactgtttca
    11641 tttacttccg atctcggtaa acccgataca cctataataa ataatccgga agtattacag
    11701 gaatgcgatt acctggtaat ggaatccact tacggtaata aaaaacatat tcttaaagaa
    11761 catcccgaag agcgtatgtt tgagattata gaggatacca tatccaacgg aggaacggtg
    11821 ataatacctt ctttttccgt gggaagaact caggagatga tttatgcatt caacgagaat
    11881 attgctttga agaagaagtt ttccatattt aacaagattc cggtgtatgt ggacagcccg
    11941 ctttcaacat tggcaacgga ggtcttcaaa aaaaatactc aatattttga tgatgaagcc
    12001 aaacaataca tattgaaagg cgataatcct ctggattttc ccaacctcat attcaccaaa
    12061 tctgtcgagg aatccaaaag actaaacttt gataattctc cgaagattat aatttcctcc
    12121 agcggaatgt gtgatgccgg aagaatacgc catcatctta aacacaactt atataaagaa
    12181 aagacaacgg tactttttat cggatatcag gcaaacggct ctttgggaag actgcttgtt
    12241 gacggagcca aaagtgttaa actgttaggt gagacaatag aggtaaatgc ccggattgag
    12301 atgatagacg gattttccgg tcatgccgac atggatgacc tggacaaata tatctctcaa
    12361 attaaggtca aaccggaaaa gattttcctg gttcacggag aagaagatca attgtacccc
    12421 tttgccgaaa gaattaataa tactttcggt attgaaacgg taataccgga aaattactat
    12481 acttatgaac ttaaaaaagg cgaaaaagtt tatgaattgg atcatgacag ggttttaacg
    12541 gagaatactt acaaaaagct taccttatta gatcttgtta ccgacgttaa gagacaggtt
    12601 aaggatgttt ccgagcttat tgcttatgat gagattaaca acctcgatga cagtcagata
    12661 acggtgctta tagatttata tgccgcaatg agacaaaata ttaaagattt gatattagga
    12721 gtggaaagta atacgaaagt tgaacgataa gatttattat tttgataatg ccgcttcgac
    12781 ttatatgtat gatgaagtga ttgagtatat aagtgaaata aacaaaaact tatatgccaa
    12841 tgcttccagt acccattttg ccggtatagc ttgccatgat atcgttaagg aaaataaaga
    12901 agcattatgt gacatactgc atattaatcc taatactctt gtgttcacat cgggtggaac
    12961 ggaatccaat aatttagcaa ttaagggaac ggcttttgcc aatttttcac ggaaaggtaa
    13021 tattgttatt acggcttatg agcatgcatc agtgataaat tccgctaaat atctggagaa
    13081 attcggtttt gagataagga cctgtccttt gaaagccgac ggaacagcgg atattgacgg
    13141 tctgtaccgt ttaattgacg ataaaacaat tttagtaagt acggcacatg tttcatcgga
    13201 gctgggaaca ataagcgata ttgaaaaaat ctcatccata attaaagccg ttaaccctga
    13261 ttgcctgtac catgtagatg cggtacagtc tttcggaaag ataagaatca atcttgcaaa
    13321 attgaatggt gtggatatga tgtctttcag cagtcataag atacacggtc caaagggaat
    13381 aggggcattg tatgtgaaga ataatgtaaa tattgatcct ctattcaacg gttcggtaca
    13441 atccggattg agagcgggta cacaaaacat atccggaata tgcggttttg cccaagcggc
    13501 aaaaattata cataaccgca ttgatgacaa ctataaccgt gtcagcggtt taaagcgtta
    13561 tttcatcgaa aatcttgctt taacaggtct tgaaacacgt ataaatactc ccgtgtgttc
    13621 aagcccgtat attcttaata tagcgtttaa ggatataaaa tcggaagtct tgcttaatgc
    13681 attatcggaa cgcgggatat gtgtgtcaag cggcagcgct tgctcttcca aatccgcaaa
    13741 aatatccgaa gtattgaaag ctatgagttt acccatagac tacatagaag gcgcaattcg
    13801 gttgagtttc tgtgaaaaga acacaaaaga ggatattgat taccttttaa agactcttga
    13861 agagcttgta ccggtactca gtaaatttat aaggaaataa tatgaagata caggcgataa
    13921 agacactgtt aataaaaccg ggcgaaatga tgttaaaagg cgataaccgt catttgttcg
    13981 aaaagcagtt aaaagataat atacaatcgg tactggacga ggtctgtaca tataatataa
    14041 ccacggaatc gggaagatat tatgtataca tcaataaatc cgactacacg gagcaggaga
    14101 ttgcatccat gctgtgtaaa gtattcggaa tagtgggtat ttccgttgcg tacagaatag
    14161 agcaggacga acagctgtta tatgagtttt tgtcacaatt cagtaagcag atatatgaga
    14221 aatccccatt ttccacattc agggtaacaa ccaaaagggc ggataaggat tttccgactc
    14281 aatccacgga agtatcaagt aaagccggtg cagtaatctt atcggtttta ccggatataa
    14341 aagtggattt attccatccc ggcattaatt tcaatattga gataagaaag aaacacattt
    14401 atgtttatca tgaagagtat aaagcgcacg gaggaattcc cgtaggcact tccggtaaag
    14461 cccttttaat gctttcaggc ggtatagaca gcccgattgc cggatatatg atggctaaaa
    14521 gagggttaca actggaagcc gtatattttc acagcgcacc gtatacatcg gaaaaagcaa
    14581 aagaaaaagt tatagatctt gcaaagatta taaaacctta cacacaactg aacagacttc
    14641 atgttgttca ctttacaaaa cctcagatgg aaatatatga aaaatgccct cataacgaac
    14701 ttacaatcat aatgagaagg gtaatgatgt atgtagccaa tgaaattgca agaatgagta
    14761 attgcaaggg aattataaca ggggagtcgt tggctcaggt agccagccag acaatagaaa
    14821 gtcttacggt taccgaccat gcggcggatt ttctggtatt gcgtcctctg ataggtatgg
    14881 ataaagcgga aattgttgat atggcaagaa aaataggtac ttttgaaaca tccattgaac
    14941 cttacgaaga ttgttgtaca atatttgtag caaagcatcc caaaacccga ccggtacttg
    15001 atcgtatttt gcagtcggag aaagttttgg atatagataa gattgtggag cattgtatac
    15061 ttaatttaga ggttgttagg atatgaaaga atttgacaag gaagataacc gcaagaattt
    15121 tgaggaattt atccaaaatg atgattatac tttggaaaga ttatgtcata aagtaggtcg
    15181 aagaaagata ataaaacaca ggagaaccga attgttactt tggctgcgtt cctttattat
    15241 agtaataatt gccgttacgg ttataagagg gttcatagga gaacccgtta aagttttcgg
    15301 accttcaatg aaagatacac tgataacagg cgatatactt gttatgagta aaataaaact
    15361 gaatactgca ggtattgaaa ggaatgatat tgtcgtgata gaaatccaac cggttcagtt
    15421 caagtattta aaattcctta atgatattaa atggttcaga agaatgtttc cttctgaaga
    15481 cagagcggac tatataaaaa gagttattgc tttggaagga gaagagattg attttattaa
    15541 cggtatcgtg tacatagacg gaaaaagact cgatgaacct tatcttaaag aatatgcatc
    15601 tacttatgaa gtagcaattg atatgccgta tacggtacct aaaggtcatg tttttgtatt
    15661 aggggataac agagctctga gtaaagacag cagaatcatg ggcgcaatac ctattgattc
    15721 aatcatcggt atagtagtat tcagaacatg gccgttagat aaattcgggg taatagatta
    15781 gaaaaaaaat tatttacata ttaaaagacc tgtgatacaa taaccgagtt gtaaaaggcg
    15841 gtccactgta tcggacggat atatgaacgc taagatgaag ggaggaacaa aaatatgagt
    15901 ttaatcaaat caatcga
//
LOCUS       DAOSOM010000038        15839 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_3726_length_15839_cov_6.222250, whole genome shotgun sequence.
ACCESSION   DAOSOM010000038 DAOSOM010000000
VERSION     DAOSOM010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 15839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15839
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3726_length_15839_cov_6.222250"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="PLF14_08125"
     CDS             complement(<1..425)
                     /locus_tag="PLF14_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71285.1"
                     /translation="MNDLITLLKADLINTYKLNGLKDKSNRKKAIGMSLLALFLTAIA
                     FLYIGGAAYLVSDILKSMGLLNILLVFAFALPFLVLFMVNIYKMPSELFSFKDYDMLM
                     SLPVKDTVILAAKVIKLMLSGYVWQFYTSFPIFLVYCIKS"
     gene            complement(418..1131)
                     /locus_tag="PLF14_08130"
     CDS             complement(418..1131)
                     /locus_tag="PLF14_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT71286.1"
                     /translation="MLRIENLSKTYANKVKAVDDISFHIEPKDMFGFIGHNGAGKTTT
                     LKCVAGILEFEKGEIYINGKSIKEDPVGCKADIAYVPDNPDVYESLTGIQYLNFVADI
                     FKVSKHDRERLTEYYAEQLTFTHALGDIISSYSHGMKQKLVIISALIHSPKLLIFDEP
                     FTGLDPQASFTVKEIMKDICNKGGAVFFSTHVLDVAEKICNKIAIIKNGKIAALGDIQ
                     SVMGDKSLESTFMELAKNE"
     gene            complement(1118..1780)
                     /locus_tag="PLF14_08135"
     CDS             complement(1118..1780)
                     /locus_tag="PLF14_08135"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOT71287.1"
                     /translation="MARINVNPTRMELNNLKRRLITARRGHKLLKDKRDELVKQFMTI
                     VKENKRLREICERMLGEAQNDFLLSRASISDQIIEESLMYPKKTIRLDVQTRNIMGVK
                     VPVITDIDEEESDPNIYPYGILSTTAELDSALYNITEVIPYLIKLAEKEKTVQLMASE
                     IERTRRRVNALEYIMIPQFEETIKYITMKLEENERGNISRLMKVKDMMIEEMRSKKDA
                     KN"
     gene            complement(1767..3164)
                     /locus_tag="PLF14_08140"
     CDS             complement(1767..3164)
                     /locus_tag="PLF14_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOT71288.1"
                     /translation="MIKEYRTINEVYGPLMIVKDVENVKYDELGEIELSNGETRKCKV
                     LEIDGTDAIVQLFESSAGINLADSKVRFLGKSLDLPVSSDMLGRVFDGMGNPIDDGPR
                     IIADKRADINGEPMNPTARDYPSEFIQTGISSIDTLNTLVRGQKLPIFSGSGLPHPAL
                     AAQIARQAKVLNVDANFVVVFAAMGITFEEADFFIQDFKKTGAIDRTVMFINLANDPP
                     IERISTPRMALTAAEYLAFEQEMHVLVILTDITYYADALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGRRKGNKGSITLIPILSMPEDDKTHPIPDLTGYITEGQIILSRELN
                     KKSILPPIDVMPSLSRLKDKGIGKGKTREDHSDVMNQLFAAYSRGREVRELAIILGEA
                     ALTETDKLYAKFAEAFENVYINQGFYENRSIEQSLDLGWKLLVKLPRSEMKRVRDANL
                     TKYYDKFKESDDGQD"
     gene            complement(3161..4933)
                     /locus_tag="PLF14_08145"
     CDS             complement(3161..4933)
                     /locus_tag="PLF14_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005358725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOT71289.1"
                     /translation="MNKGTIVKVSGPLIVAKGMKHANMFDVVKVSKHGLIGEILEMRD
                     DLASIQVYEETSGLGPGEDVVTTNMPLSVELAPGIIGGIFDGIQRPLEDMRKISGSTI
                     KRGVTLPSIDRNKKWDFKPVKKVGDFVQPGDILGVVQETVLIEHRVMVPYDISGKIVS
                     IEEGSFNVTEVIARIENEKKEIKDVIMLTKWPVRKQRPFKMKFAPDTPLVTGQRSIDT
                     FFPITKGGISAIPGPFGSGKTVVQHQLAKWSDADIVVYIGCGERGNEMTDVLTEFPRL
                     LDPRSNESIMKRTVLIANTSDMPVAAREASIYTGITIAEYYRDMGYDVAIMADSTSRW
                     AEALREMSARLEEMPGEEGYPAYLGSRLAQFYERAGKVLTSGSEERVGSISAIGAVSP
                     PGGDLSEPVTQATMRIVKVFWSLDSSLAYARHFPAINWLTSYSLYASKLDEWSDENIA
                     KNYSTLTKDAMRLLQEEANLQEIVRLVGIDALSNPERLVLEAARSLREDFLYQDAMHP
                     FDTYCSVNKRYKLLKTIMDYYYRAKEIINDDIELDKLFGMPVREKISRARLVEEQNID
                     DYFCTIDDEMEKQFKDFSDKEALS"
     gene            complement(4947..5261)
                     /locus_tag="PLF14_08150"
     CDS             complement(4947..5261)
                     /locus_tag="PLF14_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HOT71290.1"
                     /translation="MYKAAVIGRHDDILAFKAVGLTVYDVEESAFSEIVKTLERQQYA
                     VIFVTEEIYEQNTEVIGRYTDRVLPAFIAIPGVKGVTGMGMMNIKRCTERAIGADINI
                     SD"
     gene            complement(5254..6204)
                     /locus_tag="PLF14_08155"
     CDS             complement(5254..6204)
                     /locus_tag="PLF14_08155"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HOT71291.1"
                     /translation="MRNSDKYAHSIGRVRVRENFMMDEQKLARLVDAPDMKEAVRILA
                     ECRYVEDEDYMTMLYNEQKSLYAYMREILPDTKIFDAFFLKFDIHNLKVLLKAEFLKK
                     PYDEIALDTGVYKYEELKEWLHNRDFSHLPEDIAQAVQLSMEEFLKTNDPQLIDLIMD
                     KAYYTYFNRIMEKTYDRFLKDFTIIQTDLMNLKAFMRIKKVFRKEQFLNYAIIPGGSV
                     KPEDYMMLFEADEENFRKFLKQTPYASVFEKGDIEKNSDDYIMTFLRKHRYEPLGISP
                     VIGYMLGKETEIKNARITMIGKANRISRERTKERLRMCYV"
     gene            complement(6201..6806)
                     /locus_tag="PLF14_08160"
     CDS             complement(6201..6806)
                     /locus_tag="PLF14_08160"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HOT71292.1"
                     /translation="MPGIDRIYDKIIADAEERAGLRLSETSSKIQSMKSVVDERIEEY
                     RKVKISEAEKEAKGIVSRAQANRRLEGKKQLLAVKQEMVTEVFEKTQQALINLSNAEK
                     LTLYTKLIRNCLKEGHNEVSLNGKDKKAFGDKLIQNTGSALGHDISVALSGQDIPDDF
                     GVIVKNGKIYSNSTFSSMLKYMKQNIEADVMKILFKRGVEV"
     gene            complement(6807..7274)
                     /locus_tag="PLF14_08165"
     CDS             complement(6807..7274)
                     /locus_tag="PLF14_08165"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HOT71293.1"
                     /translation="MFKYVLENNLGDVLAIIGAALCFLLAGSGAAKGVSMAGKVGSGV
                     IAEDSSKFVPVMILEALPSTQGIYGFVVAFLIIGKVGGLSLEQGFSLIIAGLPAGVVG
                     LISAIFQGQVAVASIGIVAKRSADMVKGVVLTLMVELFAIFALLISILMVGKV"
     gene            complement(7293..9263)
                     /locus_tag="PLF14_08170"
     CDS             complement(7293..9263)
                     /locus_tag="PLF14_08170"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HOT71294.1"
                     /translation="MAVVSMKKICITGLLKEKKPMLSFLQKRGVVDIQDMPIDSEYEN
                     VFTKGYDKNSEAEIDSKMALLSKAISVLEEYDESKKPLFKVRKDIDRQYYESITQYND
                     EFIGLAEKVIETYNSIGDFRDEINRLENDILTLTPWTDLDIPINMSKTDKVNIVLGTI
                     PEFVKPETYTQAIDEKGLLCQVQEISRDKNNAYVATYIHESDMQQPMHYLKSIDFNIL
                     EYDFSGIPGRLIEENKQTISRLKVNIENAKSSLKEYALKTGEVQAVYDHFAISKGLLT
                     VDDLILSGKYSFIIDGWIPEEKVEKTVGALEKDFVCHVFVRDPHDDEEFPVLLKNGFL
                     GDSVTGVTEMFSLPNCREADPNTVTAIFFALFFGMMLSDAGYGLVMSVVCAVILLKFK
                     LEYATARFIKLMLISGIATIIVGALYGSWFGDLIPTILSDKSINVALWFDPIADPNRL
                     LMWSLILGVIHIYVGYGMKGYNNIKKKKYLDAVLDVLPWYFFFTMACFLVSSYIPSVP
                     ENVYMTLTPIGKKLFPYSAAVILLTQGRHKKGIFGKLFGGIAAFYDLINFMGDVLSYS
                     RLLAMGLATGVIGSIINQLGSAGGFTIPGIITFTLVFVVGHGFNFAINALGAYVHSSR
                     LQYIEFFNKFFEGGGVAYSPLFEDTKYIRIRE"
     gene            complement(9250..9582)
                     /locus_tag="PLF14_08175"
     CDS             complement(9250..9582)
                     /locus_tag="PLF14_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71295.1"
                     /translation="MGKMMEKTYLDKIKEAEEKAAGIFRDAVNKSELMLGDAEKEHSE
                     YERKSIEKIDEQCRNMLLKAEKEATDLVQKADREYAKMSEDLKTEALSNVKEAVDFVL
                     EKVTNGSR"
     gene            complement(9682..10962)
                     /locus_tag="PLF14_08180"
     CDS             complement(9682..10962)
                     /locus_tag="PLF14_08180"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOT71296.1"
                     /translation="MTLKRDGYMPRLVDSKIEKYLKIFGAVSIEGPKWCGKTWSGLNH
                     ANSVTYMTEKSQRDMANIDPKYIFLADRPQLIDEWQIVPGIWDAVRHECDSSRDKGKF
                     ILTGSTSLDSTTSDEIYHSGTGRIATIRMHTMSLYESKDSTGEASIKDMLQGQLNEGH
                     TGKVELEKLAHLIIRGGWPENIDMVEDDIGIIPKSYIDTIVTKDMHESDKRRRDSNKM
                     RMLIRSLSRNEATLASNATLVRDIEDYENKEQLIESRTTLTDYLGVLDRLFLTANQEA
                     YSLNYRSSARVGKTAKRHLVDPSLCCASLQLTKDKLLNDHETFGLMFEALVKRDLRIY
                     ADYLDGYLYHFRDNSSGDEVDAIIEFSEGNYAAFEIKLSDRGIKDAIESLSKFYTNVK
                     RKPAFMCVIVGHCEAIMKDRKTGIYIVPITSLKP"
     gene            complement(11051..12421)
                     /locus_tag="PLF14_08185"
     CDS             complement(11051..12421)
                     /locus_tag="PLF14_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004116395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT71297.1"
                     /translation="MERSALQELIQWNDNKRRKPLILWGARQVGKTYLIRDMFANTYY
                     KENNIYVDFKKDDEIREFCSQTANAKKIAEYLSLLRGKQIDENTLLVFDEIQECPNII
                     SSLKYFCQDLSEIPVIATGSMVRIKIQRETNKRGSVNNGKFLFPVGKINQMTLYPLTF
                     DEFLMNSNKMLYDVVLKAYESKTPLDSNIHELAMEQVYKYLLVGGMPEAVAAYIETDN
                     FLTSREILMELYDNYLADMQLYQASSEAILRSHALFQNIYKELNKESKNFSPGLIERN
                     SKTRDFATSIQWLTLAHIVNQSFQLKEHVTMPLIPDDLNSFRLYLADIGMFSYQSGIN
                     AASFISSERENTLSGIFFENFVANELVAKGHKLFYWRGKASAEIEFIIESNNRIYPID
                     VKKGRGVLNSLEKFSNHNKFECAIKVSGNNYGYNAEKKLLTIPFYFISFLAKDLAKGI
                     FNANKY"
     gene            complement(12645..14132)
                     /locus_tag="PLF14_08190"
     CDS             complement(12645..14132)
                     /locus_tag="PLF14_08190"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT71298.1"
                     /translation="MRNKITKKLLLYFLIIVLANSLISSGIFVVLGQKVYMDAYKEYL
                     ARRAEDIAQAISDNMDIFAYAAEEETTTPGRGKKQGGKNNPNTRVQPKYIDWMDQVLE
                     GKVWLIYKEDKIFQRGNSDIALSYDDLTDYEKAAIDKAFEGNTVTSESFDNIFDKGTL
                     SAVAPLKDSDGSIYGAILIHEDIAIARRFIDSAFYVLMVSGLVGIFIVLIMIIIFVCK
                     FIRPINRIDSAAKVMIDGDYQVRTGVNQDDEIGDLAKNMDELAVRLERGRKESENIDR
                     MRNDFISSMSHELKTPVTVMKASLEGLVSGIIPESEIENYHKILYEEIGVLDKLVMDL
                     MELNAVRNKSFPMNFKEEDLISVLKDAARSQRVLADKKGVEIILNIEDSYYMFSCDYT
                     RLRQMFITVINNGIKYSDADEQVIIRQFRDRNFIKIQVINKGNAIDSKDKEHIFEAFY
                     RGKNTSEKGFGLGLAIAKEIANRHAIHLHVFSESERETVFEFVLQ"
     gene            complement(14129..14818)
                     /locus_tag="PLF14_08195"
     CDS             complement(14129..14818)
                     /locus_tag="PLF14_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT71299.1"
                     /translation="MNRILIADDNEDIVNILKKFAEKENLDADVAFDGQQALDMFDKN
                     NYDLILLDIMMPKKDGYEVCKTIRKTSMVPIIMITAKGEDYEKIMGLDLGADDYIVKP
                     FSVAEVMARVRAILRRIEKKSGQNPALVIDSMKLDLDSYTFEVDGQNVDLTKKEFELL
                     WTLASNTGKVYTRENLLDSLWGYDYYGDSRTVDTHVKRLRAKLAAFPGLDDVIKTIRG
                     VGYKFKEPDNK"
     gene            complement(14919..15575)
                     /locus_tag="PLF14_08200"
     CDS             complement(14919..15575)
                     /locus_tag="PLF14_08200"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOT71300.1"
                     /translation="MKKITKIFITGLLTLIMIAGMSIPAMAATETELTDMVSEEMTAN
                     YLYTELAAKFPEVKVFANLAKSEMRHIAALKKAAARLGLSVEAAKPADILIPETVEDA
                     LAFAMAYELEDIEMLKVLIEKEEDAMLKRVLNNLLKGSERHYNALKRVADNGVENCVC
                     NEDGCTYQNKVNNRSKNGGQGNGQKANANAQKGMGNGQKGQGNRNNSGCTSDQCKRAQ
                     "
BASE COUNT         4211 a         3739 c         2717 g         5172 t
ORIGIN      
        1 gatttaatgc agtaaaccag aaaaatcgga aaacttgtat aaaactgcca cacataaccg
       61 cttaacataa gttttattac ttttgcggca agtatgacag tatcttttac cggtaacgac
      121 ataagcatat cgtaatcctt gaaagaaaac agttccgacg gcatcttata tatattgacc
      181 ataaacaaaa ctaaaaaagg taacgcaaac gcaaatacaa gcagtatgtt cagaagtccc
      241 atactcttca gtatatcgga gacaagataa gcggctcctc ctatatatag aaacgctata
      301 gctgtcagaa aaagagcaag cagggacata cctattgctt tttttctgtt tgacttatct
      361 ttaagtccgt tcagtttgta tgtgtttata aggtcggctt tcagaagcgt aattaaatca
      421 ttcatttttt gcaagctcca tgaaagtgct ttccaaagac ttatccccca ttacactttg
      481 tatatcccct aatgccgcta tttttccgtt cttgataatg gctattttgt tgcatatttt
      541 ttccgccaca tccaatacat gcgtggaaaa gaaaaccgct ccgcccttat tgcaaatatc
      601 tttcattatt tcttttacgg taaaggaggc ttgcgggtcc agaccggtaa aaggttcatc
      661 gaatataagc agtttgggac tgtgtatgag tgcggatatt ataaccagtt tctgtttcat
      721 gccgtgtgaa tatgaagata ttatatcacc caaagcatgg gtaaaggtca gttgctcggc
      781 ataatattcg gtaagtctct cgcggtcatg ctttgaaact ttaaaaatgt ccgcaacgaa
      841 attcagatat tgtattccgg tcagcgattc atatacatcg ggattatccg gtacatatgc
      901 aatatccgct ttgcatccga cgggatcttc ttttatgctt tttccgttga tataaatttc
      961 ccctttttca aactccagta taccggcaac acatttcagt gttgtggtct tacccgctcc
     1021 gttatgccct ataaaaccga acatatcttt aggttcaata tgaaaggaga tatcgtcaac
     1081 cgcttttact ttatttgcat aggttttgga aagattttca attcttagca tctttcttac
     1141 ttctcatctc ttctatcatc atatccttga ccttcatcag tctggatatg ttgccgcgtt
     1201 cgttttcctc taacttcatg gtgatatatt taatagtttc ctcaaactgg ggaatcatta
     1261 tatattccaa cgcattgact cttcttcttg tcctctcaat ctccgatgcc ataagctgta
     1321 cggttttttc cttttccgcc agttttatca aatagggtat gacctcggtg atgttgtata
     1381 aggcgctgtc cagttcggcg gttgtactca atataccgta cggatatata ttgggatcgc
     1441 tttcttcttc atcgatatcg gttattacgg gaaccttaac ccccattata ttccttgtct
     1501 gcacatccag tcttatggtc tttttgggat acataaggct ttcttctata atctgatcgg
     1561 aaatggatgc tctggacagt aaaaagtcat tctgcgcttc tcccagcata cgctcgcaga
     1621 tttcccgaag ccttttattc tcctttacaa tagtcataaa ctgcttaaca agctcatccc
     1681 gcttatcttt taagagctta tgacctcttc ttgcggttat aagtctcctt ttgagattgt
     1741 taagttccat ccttgtagga tttacattaa tcctggccat cgtccgattc cttaaattta
     1801 tcgtaatact tggtcagatt ggcatccctg acccttttca tttcacttct gggcagtttc
     1861 accagtaatt tccaacccaa atccagcgat tgctctatgg acctgttctc atagaatccc
     1921 tggtttatat atacattttc aaaagcttca gcaaatttgg catacaactt gtccgtttcg
     1981 gtcaaagccg cttcacctaa tataattgcc agctctctta cttccctgcc tctggaatat
     2041 gcagcgaaaa gctgattcat aacatccgaa tggtcctctc tggtcttgcc ctttcctatc
     2101 cctttatctt tcagacggga aagtgaaggc attacatcta taggaggtaa tatggatttt
     2161 ttattcagct ctctggataa tataatctga ccttccgtta tatatccggt aaggtcggga
     2221 atgggatgtg ttttatcatc ttccggcatg gacagtatag gtatcagagt tatactgcct
     2281 ttgttgcctt ttcttcttcc cgcccgctca tacatggtgg caaggtcggt atagagatat
     2341 ccgggataac ctcttcttcc gggaacttct ttcttagcgg ccgatacttc tctcaacgcg
     2401 tcggcatagt atgtgatatc ggtcagaata accagaacat gcatttcctg ttcaaatgcc
     2461 agatactccg cagccgtcag agccattctc ggtgtggata tcctttcaat cggaggatcg
     2521 ttggcaagat taataaacat aacggttctg tctatggctc cggtcttctt gaagtcctgt
     2581 ataaagaaat ccgcctcttc gaatgtaata cccattgccg caaaaaccac aacgaaattg
     2641 gcatccacat tcaggacttt tgcctgcctt gcaatctgag ccgcaagtgc gggatgcggt
     2701 aatcccgaac cggagaatat cggcaacttc tgtcctctta ccaaagtatt gagagtatct
     2761 attgaagaga taccggtctg aataaactcc gaaggataat cccttgccgt aggattcatg
     2821 ggttcaccgt ttatatccgc tcttttgtcc gcaataatcc gggggccgtc atctatgggg
     2881 tttcccattc cgtcaaacac cctgcccagc atatcgcttg ataccggcaa atccagactc
     2941 ttgcccaaaa accttacttt actgtcagca aggtttattc cggcggaact ctcaaacaac
     3001 tgaactatgg catcggttcc gtctatttcc agcactttac actttctggt ttcaccgttg
     3061 gaaagctcta tctcgcccaa ttcgtcatac ttgacgtttt ccacatcctt tactatcatc
     3121 aaaggaccgt atacttcatt aatagtcctg tattctttaa tcatgataaa gcctccttat
     3181 cggaaaaatc tttgaactgc ttttccattt catcatctat agtacaaaaa tagtcatcaa
     3241 tattctgttc ttcaacaagc cttgcacgtg aaatcttctc acgtaccggc ataccgaata
     3301 atttatccag ctcaatatca tcatttatta tctcttttgc tctgtaataa taatccataa
     3361 tggtcttcag aagtttatac cgcttattta cactgcaata ggtatcaaac gggtgcatgg
     3421 catcctgata aagaaaatcc tctcttaacg accgtgccgc ttccagaacc agtcgctcgg
     3481 gattggaaag cgcgtctata cctacaagtc tcactatttc ctgaagattc gcttcttcct
     3541 gcaacagcct catggcatct ttggtaagtg tcgaataatt ctttgctatg ttttcatccg
     3601 accattcatc caatttggat gcatagagag agtaactggt cagccagttt attgccggga
     3661 agtgtctggc ataagccaaa gaggaatcca gactccagaa cacctttaca attctcatcg
     3721 tggcctgtgt aaccggttcg gaaaggtctc ctcccggagg tgataccgca ccgatagcgg
     3781 aaatggaacc tacacgttcc tcacttcccg aagtcagaac ttttccggct ctttcgtaga
     3841 actgtgccag acgggaacct aaatatgcgg gataaccttc ttcacccggc atttcctcca
     3901 gtcttgccga catttctctc aacgcctccg cccacctgga tgtggagtcg gccatgattg
     3961 caacatcata ccccatatct ctgtaatatt ccgctatggt tataccggta tatatggaag
     4021 cttctcttgc cgcaacaggc atgtcggaag tattggcaat aagcaccgtt cttttcatta
     4081 tggattcgtt gcttctcgga tccagtaacc tcgggaattc cgtcagcacg tcggtcattt
     4141 catttcctct ttcgccgcat cctatatata ccactatatc ggcatccgac cacttggcaa
     4201 gctgatgctg tacaacggtt ttgccgcttc cgaacggtcc gggtatggcc gatataccgc
     4261 ctttggttat agggaagaag gtatctatgg acctctgtcc cgttaccagc ggggtatccg
     4321 gtgcgaactt catcttaaac ggacgttgtt ttcttaccgg ccatttggta agcataatta
     4381 cgtcttttat ctcttttttc tcattttcta tccttgcaat gacttcggtt acattaaaac
     4441 tgccctcctc aatgctgaca attttaccgc ttatatcata gggaaccatc actctgtgct
     4501 ctataagtac ggtttcctgc acaactccca gaatatctcc cggttgcaca aagtcgccta
     4561 ccttctttac aggtttgaag tcccattttt tatttctgtc tatactgggc agcgttactc
     4621 ctcttttaat ggtgctgccg gatatttttc tcatatcttc caaaggcctc tgaataccgt
     4681 caaatatacc gcctatgata cccggtgcaa gttccacgct gagaggcata tttgttgtta
     4741 ccacatcctc acccggtcct aaaccggagg tttcctcata tacctgtata ctggccaaat
     4801 catccctcat ctccagaatc tcaccgataa gaccgtgttt gcttactttt accacgtcaa
     4861 acatattcgc atgtttcatt ccttttgcga ctatgagagg tcccgatact ttaacaatag
     4921 ttcctttgtt catatgattc tccattctaa tcacttatat taatatccgc accgatggca
     4981 cgttcggtgc atctttttat attcatcata cccattccgg taacaccctt tactccgggt
     5041 atcgctataa atgcgggcaa tactctgtcg gtatatctgc ctataacttc ggtattctgt
     5101 tcgtaaatct cctcggttac gaatataacc gcgtattgct gcctctccag tgtctttact
     5161 atttcactga aggcggattc ttctacatca tataccgtca atcccacggc tttaaaagcc
     5221 agtatgtcgt catgacgacc tataacggca gctttataca taacacatcc tcagcctttc
     5281 tttggttcgc tctctgctta tcctgtttgc ttttcctatc atggtaattc ttgcattctt
     5341 tatttccgtt tccttgccca gcatatatcc tattacggga gatattccca acggctcata
     5401 tctgtgcttt ctcagaaatg tcattatata gtcatcggag ttcttttcta tatcaccttt
     5461 ttcaaacacc gaagcatatg gggtttgctt taaaaatttg cggaaatttt cctcatcagc
     5521 ttcaaaaagc atcatataat cttccggctt taccgatcct ccgggtatga tggcatagtt
     5581 taagaattgt tctttgcgga atactttttt aatcctcata aacgccttta agttcataag
     5641 atcggtctgt attatcgtaa agtccttcaa aaacctgtcg tatgttttct ccattattct
     5701 gttgaagtat gtatagtatg ctttgtccat tatgagatca atcagctgag gatcgttggt
     5761 tttcaaaaac tcttccatac tgagctgtac cgcttgtgca atatcttccg gaaggtgtga
     5821 gaaatctctg ttatgaagcc attctttaag ttcctcatat ttatacacac cggtatccag
     5881 cgctatctcg tcataaggct tttttaagaa ttccgcttta agcaagacct ttaaattatg
     5941 tatgtcgaac ttaaggaaaa aggcatcgaa tatttttgtg tcgggcaaaa tctctctcat
     6001 atatgcataa agtgatttct gttcgttata caacatagtc atataatcct catcttcaac
     6061 atatctgcac tcggcaagaa tcctgaccgc ttctttcatg tcgggagcat ccacaagcct
     6121 tgcaagcttt tgttcatcca tcatgaagtt ttctcttacg cgcactcttc ctatggagtg
     6181 tgcatatttg tcggaatttc tcaaacctca acacctcttt taaaaagtat cttcataaca
     6241 tccgcctcta tattctgctt catatacttg agcattgatg agaatgtgga gttggagtat
     6301 atttttccgt tttttactat tactccgaaa tcatccggta tatcctgccc ggaaagagca
     6361 accgaaatgt catgaccgag agcactccct gtattctgta taagcttgtc accgaaagcc
     6421 tttttgtctt taccgttcaa acttacctca ttgtgtccct ctttaagaca gtttctgata
     6481 agctttgtat acaaagtcag tttctcggca ttactaaggt ttatcagtgc ctgttgtgtt
     6541 ttttcaaaca cttcggttac catttcctgc ttaaccgcaa gcaactgttt tttaccctca
     6601 aggcgcctgt tggcctgtgc ccgggataca atgccttttg cttctttttc cgcctcggat
     6661 attttaacct tccgatattc ctcaattctc tcatccacaa ccgatttcat tgattgtatt
     6721 ttggatgagg tttcggataa ccgtaaaccc gcacgttctt cggcgtcagc tattatcttg
     6781 tcatatatcc tgtctattcc cggcatttat actttaccta ccatcagtat ggatatgagc
     6841 agtgcgaata tggcaaaaag ctctaccata agtgtaagaa ctacgccctt aaccatatca
     6901 gcgcttcttt ttgcaacgat gcctatactc gctacggcta cctgtccctg gaatattgcg
     6961 gaaataaggc cgacaacgcc tgcaggcaga ccggcaatta ttaagctgaa gccctgttca
     7021 agacttaagc ccccgacttt accgattatg aggaaggcaa caacgaaacc gtaaattccc
     7081 tgcgtgctgg gcagcgcctc taaaatcatt accggcacga atttgctgga atcttccgca
     7141 ataacaccgc ttcccacttt accggccatg gagactcctt tggcggcacc cgatccggca
     7201 agtaaaaagc ataatgccgc tcctataatt gccagcacat cacccaaatt gttttccaat
     7261 acatacttaa acataaaata atcctccgat ttttattctc tgatccttat atattttgta
     7321 tcctcaaaaa gaggagaata agctactccg ccgccttcaa agaacttgtt gaaaaattct
     7381 atatactgca acctgctgga atgcacatat gcacccaaag cgttaatggc gaaattgaac
     7441 ccgtgtccca ccacaaacac aagcgtgaat gtaataatac ccggtattgt gaagcctccg
     7501 gccgatccta actggttaat gattgaccct atgactcccg ttgccagtcc cattgctaaa
     7561 agtctggaat aagacagcac atctcccatg aagttgatta aatcgtaaaa ggccgcaatt
     7621 cctccgaaaa gctttccgaa tatacccttt ttatgtcgtc cctgtgtaag caatattacg
     7681 gctgccgaat acgggaaaag cttctttccg ataggtgtca atgtcatata tacattctcg
     7741 ggaacactgg gtatgtacga agaaaccaga aaacatgcca ttgtgaagaa aaagtaccac
     7801 ggcaacacat ccagcacggc atctaaatat ttcttcttct ttatgttatt gtatcctttc
     7861 attccgtaac ccacgtatat atgtataact cccagtatca gcgaccacat aagaagcctg
     7921 ttggggtccg ctatagggtc aaaccataaa gctacattta tggatttatc ggataatata
     7981 gtgggtataa ggtcaccgaa ccaggaaccg tataaggctc ccacaataat cgttgctatg
     8041 cccgatataa gcatcaactt tataaacctt gccgtggcgt attccagctt gaatttgagc
     8101 agtataaccg cacagactac cgacataacc agcccgtaac cggcatcaga aagcatcatc
     8161 ccgaagaaca gtgcaaagaa tattgccgtg accgtattag gatcggcttc cctgcaattg
     8221 ggcagactga acatttccgt aactcccgtt accgagtctc ctaagaatcc gttcttaaga
     8281 agtaccggaa actcctcatc atcatgagga tctcttacaa atacatgaca aacaaagtct
     8341 ttttccagag caccgacagt cttttcgact ttctcctccg gtatccagcc gtcgataata
     8401 aagctgtatt tgccggaaag tattaaatcg tccactgtca acaatccttt gcttattgca
     8461 aaatggtcat agacagcctg tacttctcct gtcttcaaag catattcttt caaactgctc
     8521 tttgcattct ctatgtttac tttaagtcgg gatatagttt gtttattctc ttcaattaac
     8581 cgaccgggaa taccggaaaa atcatattcc aatatattaa agtctatact tttaagataa
     8641 tgcatgggct gttgcatgtc cgattcatga atatatgttg ccacataagc attattcttg
     8701 tccctgctta tctcctgaac ctgacataat aatccctttt catcaattgc ctgagtatat
     8761 gtctcgggtt ttacaaactc cggtattgta cccagaacaa tattgacttt atcggtttta
     8821 gacatgttga tgggtatatc aagatcagtc cacggcgtaa gagtcagtat atcattttcc
     8881 agccggttta tttcatctct gaaatccccg atagagttgt aagtctctat aaccttctcg
     8941 gcaagtccga taaactcatc gttgtattgc gttatacttt cataatattg tctgtctata
     9001 tcttttctga ctttgaataa aggttttttg gattcatcat attcttccag aacggatatg
     9061 gcttttgaca gcaaagccat tttgctgtct atttcggctt cggaattttt gtcataacct
     9121 ttggtaaaga cattctcata ctccgaatca atcggcatat cctgaatgtc cacaacgccg
     9181 cgcttctgaa ggaaagaaag catgggcttt ttctccttta aaaggccggt tatgcaaatc
     9241 ttcttcatgc taacgactgc catttgtcac cttttccaaa acaaaatcca ccgcttcttt
     9301 aacattggaa agagcttcgg ttttcaagtc ttcactcatt tttgcatatt ccctgtccgc
     9361 tttttgcaca aggtccgttg cttccttctc ggccttcaag agcatgtttc tgcactgttc
     9421 atcaatcttt tcaatgcttt ttctctcata ctccgagtgc tctttctccg catcaccaag
     9481 catcaattcc gacttattga cggcatctct gaaaataccc gccgccttct cttccgcttc
     9541 tttgatttta tctaaatatg ttttttccat catctttccc atctgttaat cacatcaaca
     9601 tattttttat tcaacaaaag atattatatc atattaatat attgaattgc agggagaata
     9661 taaataaatc atatttaagt cttacggctt tagtgaagtt atgggaacaa tatatatgcc
     9721 cgttttccga tctttcatta tagcttcaca gtgtccgaca atgacacaca tgaatgcggg
     9781 tttccttttt acattggtat aaaattttga aaggctttct atagcatcct taatacctct
     9841 gtcgctcaat tttatctcaa aagctgcata attaccttca ctgaactcta taattgcatc
     9901 cacctcatct cccgacgagt tgtcacgaaa atgatagaga taaccgtcaa gataatctgc
     9961 atatattctc aagtcccgct ttacaagtgc ttcaaacatc agaccgaatg tttcgtggtc
    10021 attaagcaat ttatctttcg taagttgcag gcttgcacag caaagtgacg ggtcaactaa
    10081 atgtctctta gcggtttttc cgacccgtgc ggaagaacga taattcagac tgtaggcctc
    10141 ctggtttgca gtgagaaata acctgtctaa aactccgaga tagtctgtta aagttgtccg
    10201 actctcaatt agttgctctt tattttcata atcctcaata tctctgacca atgtagcatt
    10261 acttgcaagt gttgcttcat ttctcgacaa agaacgaata agcatacgca tcttgtttga
    10321 gtctcttctt cttttatcac tttcatgcat atcctttgta acaatggtat caatgtagct
    10381 tttaggaata atacctatgt catcttctac catatcgata ttttccggcc aacctcccct
    10441 gataatcaaa tgtgccagtt tttccaattc aactttacct gtgtgaccct cgttcaattg
    10501 accttgcaac atatctttaa tagaggcttc gccggtggaa tcctttgatt cgtacaaact
    10561 catagtatgc atacgaatcg tagctatcct tcctgttccg gaatggtata tttcatcact
    10621 tgttgttgaa tcaagagaag ttgaacctgt gagtataaat tttcctttat cccggctact
    10681 gtcgcattca tggcgaaccg catcccatat accgggaaca atttgccact catcaattaa
    10741 ttgcggtctg tcagcaagga aaatatattt cggatctata tttgccatat ctctctgact
    10801 tttttcagtc atgtaagtta cactgtttgc atgattcaac cccgaccagg tctttccgca
    10861 ccatttggga ccttctatgg aaaccgctcc gaagattttc aaatatttct caattttact
    10921 atcaaccagt ctcggcatat atccgtctct ttttaaagtc atgacaaccc tccgagaaca
    10981 taataacact tcattgtgca cttttcaagt gtttcattgt acacttttca gcattttcat
    11041 tgtgcacttt tcagtatttg ttagcattaa atatgccttt tgcaagatcc tttgctaaaa
    11101 atgagataaa gtaaaaaggt attgtgagca gctttttttc cgcattataa ccatagttat
    11161 tcccggaaac ctttatggca cactcaaact tattatgatt ggaaaacttt tctaaagaat
    11221 taagtactcc tctacctttt ttaacatcaa tcggatatat tctgttattt gactcaataa
    11281 taaattctat ttcagcagat gccttccccc gccaatagaa aagtttatgc ccttttgcta
    11341 ccaattcatt tgccacaaag ttttcaaaga agatacctga taatgtattt tctctttcgc
    11401 ttgatatgaa tgaagctgca ttaataccgc tctggtatga aaacatgcct atgtcggcaa
    11461 gataaagacg aaagctgttt aaatcatccg gaatcagcgg catagtcaca tgttccttca
    11521 gttggaatga ttggtttaca atatgagcca atgtgagcca ctgaatggaa gtagcaaaat
    11581 cccttgtttt gctattcctt tcaataagtc cgggagaaaa attctttgat tccttgttca
    11641 gttccttgta gatgttttga aataacgcat gtgaacgtaa aatagcttcc gaactggctt
    11701 gataaagttg catatccgca agataattgt catacaattc cattaatatt tctcttgatg
    11761 tcaggaaatt gtccgtttct atataggcag ccacagcttc cggcatacct cccacaagaa
    11821 gatatttata tacctgctcc atagctaatt catgaatgtt agaatccaga ggagtcttac
    11881 tttcatatgc tttcaaaacc acatcgtaaa gcattttatt gctgttcatg agaaattcat
    11941 caaaggtcaa aggataaagt gtcatttgat ttatctttcc gacaggaaaa agaaactttc
    12001 cgttattaac ggaacctcgt ttatttgttt ctctttgtat ttttattcgt accatagaac
    12061 ctgtcgcgat aacaggaatt tcacttaagt cttggcagaa atatttaagt gaagatatga
    12121 tattgggaca ctcctgtatc tcgtcaaaaa ccagcagtgt attttcatct atttgctttc
    12181 ctctcaacaa agataaatat tccgctattt tcttcgcatt tgccgtctgc gagcagaatt
    12241 cccgaatttc gtcatctttt ttaaagtcaa cataaatatt gttttcctta taataagtat
    12301 ttgcaaacat atctctgata agataggttt taccgacctg tcttgctccc caaagaataa
    12361 ggggctttct tcgcttgtta tcattccact ggatcagttc ttgtaaagcg ctgcgttcca
    12421 tattgaatct ccttgctttt aataatgcca ttatagcata acttgcacca atttacatgt
    12481 tgttttacga cttatttgca ccttttgcag ggtttacggc ttaattattt gcacctgcac
    12541 cgattttaag tattattaca aagcaaaaat aatcatatac attacaattg tcccgaatac
    12601 gatgctgttt tcgggacata atattttatg attgctattc tttgctattg aagcacaaat
    12661 tcaaaaacag tctctctctc cgattcgctg aacacgtgga gatgaatggc atgacgattg
    12721 gcaatttcct tggctatggc caatcccaga ccgaatcctt tttccgaggt gttcttaccc
    12781 cggtaaaatg cttcaaatat atgctcctta tccttggaat ctatagcatt gcctttgttg
    12841 ataacctgta ttttaataaa gttgcggtca cgaaactgtc ggataatcac ctgctcatcg
    12901 gcatcggaat actttattcc gttattaata accgttataa acatctgtct caatcttgtg
    12961 taatcacagc tgaacatata gtaggagtct tcaatgttca atattatctc aacacctttt
    13021 ttatccgcta aaaccctctg acttctggca gcatctttca gaacggaaat aagatcttcc
    13081 tctttaaaat tcatggggaa actcttgttt cgtactgcat tgagttccat caaatccatt
    13141 accaacttgt ccaatacccc gatttcctca taaagtattt tatggtaatt ctctatctcc
    13201 gattcgggga taataccgga aaccaaacct tccagtgaag ctttcatgac ggtgacaggc
    13261 gtcttaagtt catgggacat actggagata aaatcatttc tcatgcgatc tatgttctcg
    13321 ctctccttgc ggcccctttc cagtcttaca gccaattcat ccatattttt tgccaagtca
    13381 cctatttcat catcctgatt tacgccggtt ctgacctgat aatcgccgtc aatcattact
    13441 ttagccgcac tgtcaatacg attgatgggg cggataaact tacatacaaa aatgataatc
    13501 atgataagta cgataaagat accgacaaga ccggaaacca taagaacata gaaagccgaa
    13561 tcgataaatc ttcttgcaat agcaatatct tcatgaatca atatggctcc gtaaatcgac
    13621 ccgtcggaat ctttcagggg agcaaccgcg gacaatgtcc ctttatcaaa gatattgtca
    13681 aaagattccg atgtaacggt attaccttca aatgccttat ctatggccgc tttttcatag
    13741 tcggttaaat cgtcatacga gagtgcgata tcggaattac ctctttgaaa aatcttatcc
    13801 tctttataga ttaaccagac tttgccttcc agcacctgat ccatccaatc gatatatttc
    13861 ggttgaacac gcgtattggg attgtttttt ccgccttgct tcttgccgcg tcccggggtt
    13921 gtcgtttcct cttccgccgc ataggcaaag atatccatat tgtcggatat cgcctgagca
    13981 atatcctcag ctcttctcgc caggtattcc ttgtaggcat ccatatatac tttctgcccc
    14041 agaacaacaa aaatcccgct tgaaatcaat gagttggcga gtacgattat aaggaaatat
    14101 aaaagaagct tctttgtgat tttattcctc atttgttatc cggctcctta aatttatatc
    14161 ccactccgcg aatggttttt atcacatcat ccaatccggg gaaagctgcc agtttagctc
    14221 tcagacgttt aacatgagtg tccaccgtcc tggaatctcc gtagtagtca taaccccaca
    14281 aagaatccag aaggttttct ctggtataga cttttccggt attggaagcc aatgtccaca
    14341 gcaactcgaa ttctttttta gtcagatcga cattttgccc gtccacttca aaagtatagg
    14401 aatccaggtc cagtttcatg gaatctataa ccaatgcggg attctgaccg gatttctttt
    14461 caattcttct taagatagcc ctgacccttg ccattacctc ggcaacggaa aacggtttga
    14521 caatatagtc gtcggcaccc agatccagac ccataatctt ttcataatcc tcccctttgg
    14581 ccgttatcat tataatgggg accatggaag tcttgcggat ggtcttgcaa acctcatagc
    14641 cgtccttttt aggcatcatt atatccaaca gaatcaaatc gtaattattc ttatcaaaca
    14701 tatccaatgc ctgttgtccg tcaaaggcca catccgcatc caaattctct ttttcggcaa
    14761 actttttaag aatattgaca atatcttcat tgtcatctgc gattaatatc cgattcatgg
    14821 caacctccac accatattta tacaccaaag gacagggttt ttaaaccctg cccttgattt
    14881 ttatcggcaa ttgaattaaa tgtcagttgc attaccgcct actgtgctct cttgcactga
    14941 tccgatgtgc agccggagtt gttgcggtta ccctgcccct tctgaccgtt gcccataccc
    15001 ttttgtgcat tagcattagc tttctgacca ttgccctgac cgccgttttt gctccggtta
    15061 ttgactttgt tctgataagt gcatccatct tcgttgcaga cacagttctc aacaccgtta
    15121 tcagctactc tcttcaaagc gttatagtgc ctttccgatc ctttaagaag attatttaga
    15181 actctcttca acatcgcatc ttcttccttt tcgatcagta ctttaagcat ctcgatgtct
    15241 tccaattcat aagccatcgc aaaagccaaa gcatcttcca cggtttcggg aattaaaatg
    15301 tccgcaggct tggcagcctc taccgacagt cccagtcttg cagcagcttt cttgagagcc
    15361 gctatatgac gcatctcgga cttggctaag ttggcaaaca ccttgacctc cgggaatttt
    15421 gcagccagtt ccgtataaag ataatttgcc gtcatttctt cactgaccat atccgtcaat
    15481 tcggtctcgg tggcagccat tgccggtata ctcatacctg ctatcattat caaggtcagt
    15541 aatcctgtta taaatatttt agttattttt ttcattttca gtttctcctt tcttaactgt
    15601 gccctcatta tatttccctt ctatgtcata caaagggctg ttatgtgtct ttttcgtcaa
    15661 ctttttcaac tttaacaata aataggctaa aagttcaaat tgacatatat agtctttttt
    15721 aaaagataaa tacatgcgaa tcttggaaaa gtgtcaattt ggaggtataa tagcattgga
    15781 aaacgcacaa accgggtata ggagcgttta catgcaatac aaaacagaat gttaaatac
//
LOCUS       DAOSOM010000039        15522 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_3818_length_15522_cov_5.257257, whole genome shotgun sequence.
ACCESSION   DAOSOM010000039 DAOSOM010000000
VERSION     DAOSOM010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 15522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15522
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3818_length_15522_cov_5.257257"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="PLF14_08205"
     CDS             complement(<1..1008)
                     /locus_tag="PLF14_08205"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HOT71301.1"
                     /translation="MKRSLLVITVILFICILVLGGCKTPDTSITVPASTDESDIDILP
                     ETFTLLIYLCGSDLESRSGKASANIAEMQEAEIDKNINVVVQTGGSTSWKDIEVSAQS
                     TDRFLIKTGGKQMIDRITVKRNFGDSKTLSEFIEFGVERYPADKYGLILWNHGAGSVK
                     GVCFDANFGYDGLTLSEIKEALESTKEIIGKKFEFIGLDACLMATYDMACIIEPYADY
                     MIASQEIEPSSGWDYKRLISNLGKETFYADVLNSYAEKQAQKNIYTLSAVKLSELSKA
                     EAVIDELINQINCDLSYVGVAIEEGKEFGVKGISRSTNLFDLGLLAESLGIEYDFSDF
                     II"
     gene            complement(1009..3993)
                     /locus_tag="PLF14_08210"
     CDS             complement(1009..3993)
                     /locus_tag="PLF14_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71302.1"
                     /translation="MKTGIKTRILSILLIICILLSLTACKEKKEKEPEAEVYYTAISV
                     QYSPEGQYSGTLTFDEGIEDSFTELSKEDFVLTAFYSVDEGKFSSQLEDFTVTKTGDK
                     SLTFTFKSPYGYDDNISYVLMSETAVTAKEKKIAAVVYTHAPEVDLNIEYIGLYKGTD
                     KATVTAMLGEGWKFVEKMTSDMLTFPIGLDVSVEVRRESDTKAVFVITDIPKNYTGAV
                     ISGVLSASAVDSEFSEDTDLCLDFLQPNIYIDPSTVSFDKNTNVFAVGKATLSEEFAG
                     VKDGISVWSGLYSVIEQSYDSGNNAYSFKLKINDKLDKGLDVNDVLTGITVNAVLKTE
                     SEEINYAFRPYNAIAGVKGDVITDTEKGKVYIEIVPYNATFKEGISVNDITVFGAGSL
                     SSFVCSSVASDKIVYTADYSSKLKSGVALSFNMAGRVLNTDFGLDSYSLTVPVPPYYD
                     DKDFSWGELGVTLAKSAAGSLGGAIGSAVAGFVLPHVYELLGVDTSNPELNAIRNSID
                     RLDKAIVNLNNDIRKVRVDVQVGTSMTALSELQSLQNNLLTDSRTLLGKETVINYVRD
                     VSKAAQVHSAWNYDKFCEYLAEEYGSIERARYKWTADMGTDKDLTEIYDILVSFANYT
                     NLSFSDYMDRAHNRIWGIGFSFKDMDEYQYYLGRLGEDAAKMYRCMEALREKLKGAPY
                     YGRKVKNVEIDPILTEGFINEVKELNIGNGYLQKVINLSSLILETASGTGDSIIDLFF
                     QVVDTQFNFESQTINIKKAFLAKLQSTYLISSAIALQYCDATNDGNASTIRSYIPIVM
                     EKIDNAFNKIDIMTALADKGADRLLVSGQVVSKKMKKATGTKGAKSDTSIRNDIREGF
                     LSVSDQTYTTMIERAQNRGLSLAADLQAAGFVDVVPDEGITYIYMTGNATYYNTLTVG
                     DLFVLSMKLAVTSSTDSDGGFKVTTVTQNGTGNPSVEENNVIQVDYSKTPVVWAMAFG
                     GAEGEYYTVPSYNAVIGFVSGE"
     gene            complement(4318..5127)
                     /locus_tag="PLF14_08215"
     CDS             complement(4318..5127)
                     /locus_tag="PLF14_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008819336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT71303.1"
                     /translation="MEFPKQLKDIAYRLLQEPTLDTFREFLHTQMGEHNAIDFKRQWI
                     ENAALAKEMLALANSKGGFIVFGVAENEDGSVCIEGLPEIRDKAVISNDIKNFISSNL
                     KYEIYDFSYTTSNYEALKDKKFQMLVVEDVPEHIPFLSKKESGSLKQNTIYVRRGTSC
                     EMADEEEIQAMLKRRINYLYPSKGEPLQLEEHLKQLKILYGMIEKDHVYYKNIPLNEK
                     FFSWTSITKLFFQGEKIVEANPLYPDESYEQFVSRMIVEKKKKIERVLDIF"
     gene            complement(5947..6681)
                     /locus_tag="PLF14_08220"
     CDS             complement(5947..6681)
                     /locus_tag="PLF14_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003149358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOT71304.1"
                     /translation="MLENKNKGTNEIAILMAAGMGKRMAPLTEKIPKPLVKVFGKPMI
                     ETIIDGLVERGVKHIYVVVGYLKEQFDFLLQKYENLFLIENTEFRTINNISSIHAVAP
                     IMGKEDCFICEADLYVADSSIFQAELKHSCYYGKMVKGHSDDWVFEQDENGRIIRVGK
                     YGYDNFNMCGIAWFKAKDAKIIADAIVEAYKYPGTYENLFWDDIVNQQIKNVNLIVHE
                     VFHNQITEIDSIAELEMVDPDYQKYN"
     gene            complement(6771..7895)
                     /gene="aepY"
                     /locus_tag="PLF14_08225"
     CDS             complement(6771..7895)
                     /gene="aepY"
                     /locus_tag="PLF14_08225"
                     /EC_number="4.1.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004072699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033980 - phosphonopyruvate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonopyruvate decarboxylase"
                     /protein_id="HOT71305.1"
                     /translation="MKVEKLIEIIGSDFYTGVPDSQLKALCNCLIAKYGIDPHHHIIA
                     ANEGNCTALAAGYHLATGKIPVVYLQNSGEGNIINPVASLLNDKVYAIPVVFVIGWRG
                     EPGIHDEPQHIFQGEVTIKLLNHMGIANFIISKDTSDDEVEEKMAEFRSILNQGRNVA
                     FVIRKGALTYAPNVEYTNNNKMVREKIIQHILKISEKDPIVSTTGKASRELFEARVAN
                     GQSHKFDFLTVGSMGHSSSIALGIAINKPKQRIWCIDGDGAVLMHMGSMAVIGANKPR
                     NIVHIVINNEAHESVGGMPTVASRIDFVAIAKGCGYPNAVCVDNYDDLDRELELAKNR
                     RELSLIEVKCSIAARENLGRPTTTALENKEIFMDYLKTLQ"
     gene            complement(7922..9217)
                     /gene="aepX"
                     /locus_tag="PLF14_08230"
     CDS             complement(7922..9217)
                     /gene="aepX"
                     /locus_tag="PLF14_08230"
                     /EC_number="5.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050188 - phosphoenolpyruvate mutase
                     activity [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate mutase"
                     /protein_id="HOT71306.1"
                     /translation="MKTVYTCFCTDVIHDGHLNIIKEAKKRGKVIVGCLSDEALIRYN
                     KFPTISQEERIKLYKDIDGVEEVVIQNNMLYDDVIPIIQPDYVVHGDNWLNGPERAIR
                     DNVEELLKVYGGELVDIPYTRNEKVKKIDVQLREKLAMPEYRRKRLRQLISIAPVVKV
                     LEAHSGLTGLIVEKTVIEHDGKLDQFDAMWISSLCDSLSKGKPDIELVDMTSRFRTID
                     DIMEVTTKPIIFDGDTGGLTEHFVYTVRTLERMGVSAVIIEDKKGLKKNSLFGTEVEQ
                     TQATIEEFCEKIAAGKKAQLTDDFMIIARIESLILEKGMEDALKRSRAFVRAGADGIM
                     IHSRKKDPAEILEFCDRFREEDKVTPIVVVPSSFNTITEEELIKHGVNIVIYANQLTR
                     SAFPAMQQTAEDILKYHRAKEVDDRLMPINNIITLINEL"
     gene            complement(9230..10363)
                     /locus_tag="PLF14_08235"
     CDS             complement(9230..10363)
                     /locus_tag="PLF14_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate aminotransferase"
                     /protein_id="HOT71307.1"
                     /translation="MEAIKRNILLNPGPSTTTDTVKYAQVVPDICPREKEFAGLMKGL
                     REDLVRIVHGDLEKYTSVLFCGSGTINIDVVINSLLPKGKKVFVVNNGAYSTRAVEIC
                     KYYGLPHIDLQFPVDQQPDMEKVEEILKNNKDIALVHTTHNETGTGLLNPIREIGALA
                     HKYGAIFTVDTTSTYAMRPIDIEKDNIDFCMASAQKGLMAFTGLSFVVGNRAIIEKSK
                     DYPKRSYYCNLFQQYDYFERTGEMHFTPPVQTIYATIQALKEYWAEGEEGKWARHTRV
                     FNAINKGLDELGFRQVIKPEWRAGLVSSAIYPDDSNWSFEKIHDYCYERGFTIYPGKI
                     STTNTFRLCALGAIDEADIEDFYVVFKEALAMSGVKTPVVYKD"
     gene            complement(11945..13042)
                     /locus_tag="PLF14_08240"
     CDS             complement(11945..13042)
                     /locus_tag="PLF14_08240"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde reductase"
                     /protein_id="HOT71308.1"
                     /translation="MQRIIRTENTIIELEKILKDLNVTKYFLVCGKSIKKHDIYDFFI
                     KDFRLVGIFNEYTSNPKLEDICRGVEKYKVSGSNCIICVGGGSSIDVAKCIKIFSNID
                     NPDDYLKKINIDSDDTIIAIPTTAGSGSESTKFAVVYVDNEKKSVESDIILPQYAILN
                     SNYLSQLPMKHKVCTMMDAMCQSIEAWWSVNSTEQSIEYSKEALMLIVSNMDNYLTTD
                     DKEASDRILLAANLSGRAINIAKTTATHAMSYKLTTLYNIPHGCSVAVLLPKIWGYMV
                     DNIDKCTDKRGIEYLNNVFFSIANCFGVDNPKAAIGKIEGIIEKNNIKSPIVKKSDFL
                     QLVKSVNIERLRNNPVYISEDDICSLYSSLC"
     gene            complement(13048..15054)
                     /gene="aepX"
                     /locus_tag="PLF14_08245"
     CDS             complement(13048..15054)
                     /gene="aepX"
                     /locus_tag="PLF14_08245"
                     /EC_number="5.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006848642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050188 - phosphoenolpyruvate mutase
                     activity [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate mutase"
                     /protein_id="HOT71309.1"
                     /translation="MKALILNSGLGRRMGVLTSEHPKCMTEITNTDTILSRQLKQLVD
                     VGIEDVIITTGLFDEVLVKYCESLDLSLKYTFVKNPVYDKTNYIYSMYCAREYLNDDI
                     LLMHGDLVFENSVLDKVMDFNGSCMTISSTVSLPEKDFKVQLRDGYISTVAVNVFENA
                     YSAQPLYKLKRRDWLVWLDKIINFCENGQTNCYAENAFNEVSEKCLIKPLDVKNELCN
                     EIDTPEDLLVITKRLKAVMNRDVYMCFSTDMLHSGHMAILKKASRLGRLTIGVLSDEA
                     IANFKRYPLMPFEERKTLFENIVGVNRVVLQEELSYRKNLEKYKPEYVVHGDDWKEGF
                     QKPIRDEVVEILSTYGGKLVEFPYSKDKRYDELERSAREHLSIPDIRRGRLRKLLSVK
                     HLVTAIEAHNGITGLIAENTKVYQDGKAYQFDAMWVSSLCDSTSKGKPDIELVDMSSR
                     LRTIDDIMEVTTKPIILDGDTGGLVEHFIYNVRTLERVGVSAIIIEDKTGLKRNSLFG
                     SDVIQTQDDIENFCTKIKAGKNAQKTKEFMIIARIESLILDKGMDDALSRAFAYVNAG
                     ADGIMIHSRKKEPDEVFEFVELFRKKDKNTPIVVVPTSYNTVTEEELEKKGVNIVIYA
                     NHLTRSSFPAMQKVAMTILENHRAYEADKMCMPIKDIISLIPEE"
     gene            complement(15055..>15522)
                     /locus_tag="PLF14_08250"
     CDS             complement(15055..>15522)
                     /locus_tag="PLF14_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016287776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOT71310.1"
                     /translation="QSHKYDFLTVGSMGHCSSIAFGIALQKKKTKIWCIDGDGAAIMH
                     MGSMAKIGSNKPSNLIHIVINNNSHESVGGMPTVGGQIDFVKIAQGCGYEYTISVDNY
                     ADLEIELNRVKKMNKLVFFEVKTAIGSRSNLGRPTKSPVENKEDFMQYLKECE"
BASE COUNT         4608 a         3353 c         2251 g         5310 t
ORIGIN      
        1 gataataaaa tcggaaaagt catactctat acctaaagat tcggcaagaa gacctaagtc
       61 aaacaaattt gtactgcgac ttattccctt tacaccgaat tcttttccct cctcaattgc
      121 aactcctaca tatgataaat cgcagtttat ctgatttata agttcatcga taaccgcttc
      181 cgcctttgat aattccgata actttacagc ggacagtgta tatatgtttt tttgagcctg
      241 cttttcggca tatgagttca gtacatcggc atagaaggtt tctttaccca aattggaaat
      301 aagcctttta taatcccagc ccgatgacgg ctctatttcc tgagaagcta tcatatagtc
      361 ggcatatggc tctataatac aagccatatc gtatgtagcc atcaggcagg catcaaggcc
      421 gataaactca aattttttac ctataatctc cttcgttgat tccaatgctt cctttatttc
      481 cgaaagcgtc aatccgtcat atccgaagtt tgcatcaaag catacacctt taacggagcc
      541 tgctccgtga ttccacagaa taagcccgta tttatctgcg ggataccgct ccaccccgaa
      601 ctctataaac tccgaaagag tcttactgtc tccgaagtta cgctttacgg tgattctgtc
      661 aatcatctgt ttaccgccgg tttttatcaa gaacctgtcg gtagactgtg cggatacctc
      721 tatatccttc cagcttgttg aaccgcccgt ctgaacgaca acattgatat tcttatcaat
      781 ttcagcttcc tgcatttctg caatattggc acttgcttta ccggaacggc tttccaaatc
      841 gctgccgcaa agatatataa gtaaagtgaa ggtttccggt aagatatcta tatcgctctc
      901 atctgttgat gcaggtaccg taatacttgt atcgggggtt ttacatcccc ccaatacaag
      961 tatgcaaata aacagtataa ccgttataac aagcagagag cgtttcattt attctcctga
     1021 tacaaagcct atgacagcat tatatgaggg aaccgtgtag tattcacctt cagcaccgcc
     1081 gaaagccatt gcccacacaa ccggtgtctt cgagtaatcg acttgtatga cattattttc
     1141 ctctacagaa ggatttcccg taccgttttg agttacggtg gttactttaa acccgccatc
     1201 ggaatctgta gaactggtca ctgcaagttt cattgacaag acaaaaagat cccccacggt
     1261 cagagtattg tagtaagttg cgtttccggt catatatata taagtgattc cttcatccgg
     1321 tactacatca acaaaacctg cggcttgcaa atcagctgcc aaactcaaac ctctgttttg
     1381 agctctctct atcatggtgg tgtaagtttg gtcggaaaca gacagaaaac cttcccttat
     1441 atcatttctg atagaagtat cacttttggc tcctttagta cctgttgcct ttttcatttt
     1501 tttggaaacc acctgtccgg aaaccagtaa tctgtcagcc cctttgtctg ccaaagcagt
     1561 catgatgtca attttattga atgcattatc aatcttttcc attacgatgg gaatatatga
     1621 acggatagtg gaggcattac cgtcattagt cgcatcacaa tactgtaatg ctatagcgga
     1681 gcttatgaga taagtgcttt gcaatttagc caaaaatgcc tttttaatat ttatcgtctg
     1741 cgattcgaaa ttgaattgtg tatccactac ctgaaagaaa agatcaataa tgctgtcccc
     1801 tgttccgctg gcggtttcaa gtatcagcga gctgagattt ataactttct gcaaataccc
     1861 gttaccgata ttcaactcct ttacctcgtt tataaaacct tcggtgagaa taggatcaat
     1921 ctccacattt tttactttcc ttccatagta cggtgctcct tttagttttt cgcgtaaagc
     1981 ttccatacat ctgtacattt ttgcagcatc ttcgccaagt cttccaagat aatactggta
     2041 ttcatccatg tctttgaaag aaaaaccgat tccccaaatt ctgttatgtg ctctatccat
     2101 atagtcgcta aagcttaagt ttgtataatt cgcaaaggaa accaatatat catatatttc
     2161 agttaaatcc ttgtcggtac ccatgtcagc tgtccattta tatctggccc tttcgataga
     2221 cccatactcc tcggcaaggt attcgcagaa tttatcataa ttccaggcgg aatgtacttg
     2281 tgccgccttg gaaacatcgc gtacataatt gataacagtc tccttaccta aaagtgtccg
     2341 agagtcggtc aacaaattgt tctgtaatga ttgtaactca ctcaaagccg tcatgcttgt
     2401 gccgacttga acatcaaccc tgacttttct tatatcattg ttaaggttaa caatcgcctt
     2461 atccagtctg tcaatggaat ttcttatcgc attcaactcg ggattggaag tgtcaacccc
     2521 cagaagttca taaacgtgag gtaatacaaa tccggctaca gcacttccta tcgcaccgcc
     2581 caaactgcct gccgcactct ttgcaagtgt aacaccgagt tctccccaac tgaaatcttt
     2641 atcgtcgtag tagggcggaa ccgggacggt aagcgagtag gaatccagac cgaagtcggt
     2701 atttaaaacc cgccccgcca tattaaaaga cagagcaaca ccgcttttaa gcttagaaga
     2761 ataatctgcg gtatatacaa tcttatccga agcaacggag gaacatacga aagaactaag
     2821 actgccggca ccaaaaaccg ttatatcatt tacggaaata ccctctttga aagtagcatt
     2881 atacggcact atttcaatat atactttccc tttttccgta tcggttatga catcaccctt
     2941 tacgcctgca attgcgttat acggtctgaa tgcataattt atctcttcgc tttcggtttt
     3001 cagcacagca tttaccgtta taccggtaag cacatcgttt acatccaaac ccttgtccag
     3061 tttgtcattt atttttaatt taaaagaata tgcgttattt ccactgtcat acgactgttc
     3121 aattacggaa taaagaccgc tccaaacact gattccgtcc tttacaccgg caaactcctc
     3181 tgagagtgtt gctttaccta cggcaaatac atttgtattc ttatcaaaac ttactgtaga
     3241 aggatcgata taaatattcg gttgtaaaaa atccaggcac agatcggtat cctcggaaaa
     3301 ctccgaatcc accgcacttg cacttaaaac accacttata accgcacctg tatagttttt
     3361 aggaatatcg gtaattacaa aaacagcctt tgtatcactc tctcttctta cttctacaga
     3421 tacatccaat cctataggga aagtaagcat atcggaagtc attttttcaa caaacttcca
     3481 gccttcaccc agcattgctg taacggtagc tttatctgtt cctttgtata gtccgatata
     3541 ttcaatattc aagtctacct cgggtgcatg tgtataaaca actgcagcta tctttttctc
     3601 ttttgcggtt acagccgttt cggacattaa cacatatgaa atattgtcat cataaccgta
     3661 aggagatttg aaagtaaaag taaggctctt atcacccgtt ttggtaacgg taaagtcctc
     3721 taactgcgag ctgaatttcc cttcgtctac actataaaaa gccgttaaca caaaatcctc
     3781 tttacttaat tcggtaaacg agtcctctat cccctcatca aaagtcaaag ttccgctgta
     3841 ttgtccttcg ggactgtatt gtacggatat tgccgtatag tatacttccg cctcaggttc
     3901 cttctctttt ttctctttgc aagcagtcaa tgataaaagt atgcagatta taagaagtat
     3961 acttaaaatt cttgttttaa ttcctgtctt cataatgttc ccccctataa taaataatca
     4021 tcaaaacata ttatttaaat ttgtcacaac acataaaaca aaaggttgca tggtatacaa
     4081 aaaaatgtat tccattccac atttttccaa actgattcta tatctcccaa agcttatttg
     4141 tcaatcacta ctaacaagac aacctaactg tgagaaacat ccacatttaa tttccttctc
     4201 aaacatcttt atattagcag aagaagattt atgagtttca aattcatcca tacaaattac
     4261 ctcaataaac tccctgttta ctttaccaaa taaccttgct gtaatatatt atagtattta
     4321 aaatatatca agaacgcgtt caattttttt ctttttttca acaatcattc tcgaaacgaa
     4381 ttgttcatag ctttcatcag gataaagggg atttgcttca acaatcttct ctccttgaaa
     4441 aaacaattta gtaatagaag tccaactaaa aaacttttca tttaaaggga tgtttttgta
     4501 atatacatga tctttttcga tcattccata aaggattttc aattgtttta aatgctcttc
     4561 taattgaagt ggctctcctt tcgatggata taaataattt attcgtctct ttagcattgc
     4621 ttgtatttct tcttcatccg ccatttcaca agaagttcct cggcgaacat aaatcgtatt
     4681 ttgtttaagt gacccacttt ccttttttga tagaaaaggg atgtgttctg gaacatcttc
     4741 tacaactagc atttgaaatt tcttatcctt taatgcttcg tagtttgatg ttgtatatga
     4801 aaaatcataa atttcatatt ttaaatttga tgatataaaa ttctttatat cattggagat
     4861 aacagctttg tctctaatct caggcaagcc ttcgatacat acactaccat cttcgttttc
     4921 tgcaactcca aatacaatga aaccaccttt tgaatttgct aaagctaaca tttcttttgc
     4981 taaagcggca ttttcaatcc actgtctttt gaaatcaata gcattatgct cacccatctg
     5041 tgtatgcaaa aactctctaa atgtgtcaag tgtaggttct tgtaacaatc tatatgctat
     5101 atcttttaac tgttttggaa attccatagg tgttctcctt tatcgtaaat aaaaatctat
     5161 ttaactgtaa aataataatt gcacatctta ggcttattct acactaaaaa ttctaattta
     5221 accactgtat taataaaatg tttctctcgc taaaatccaa tgctgtatag tcgcaggatt
     5281 tctcgatatt tggtcataat ctttgatggc tgcctccttt atgtgtattt gtatcaagag
     5341 attcatgaaa aacagtatat ggggaaaaga tcaataaagc cacctaaaca ggtggctctc
     5401 agcgaacgga actgtggctc tcgggcaacg gaagggtggc tctcaaactc cggacaactg
     5461 gctcaaaatg gtccggaata ttcacaattg tttaattcga cttcaagttc attacacagt
     5521 tgtatctttt acaagaacac cgacctctat ccaaacatat ttattaaaat aaaaaaaagg
     5581 aagcccgttt tcgaacctcc tttataccgg ttattaatat ttcttaaaat agaaattatc
     5641 ttgttgtact cttatcaaat tcagtcaata cataatcttt tccgtcttcc gacatggtaa
     5701 gcatgtatat ctcaaaagcg cttccgtttt cagcagctat actgaccagt atgagtgctt
     5761 atccaatcct ttaataattt agcagtactc taaacacaaa agtctggcat ttttagatat
     5821 ttgaaaacat tcatcattaa ttcgcctata atctctaagg gacctactaa attacaaaac
     5881 tctttcacct ttcatttgct aaatccatat atgaaatcct catagtcgca gaagctatga
     5941 gcttcattag ttgtacttct gatagtcagg atcaaccatc tcgagttctg caatggaatc
     6001 aatctctgta atctgattat gaaaaacttc atgaacgatg agattaacat tctttatctg
     6061 ctgattaaca atatcatccc agaaaagatt ctcgtatgtt ccggggtatt tataggcctc
     6121 tacaatggca tcggcaatga ttttcgcatc tttcgccttg aaccatgcaa taccacacat
     6181 attaaaattg tcgtagccat acttcccgac tcggatgatt ctaccgtttt catcttgttc
     6241 aaacacccag tcatcactgt gtcccttaac cattttaccg taataacagg aatgtttgag
     6301 ttctgcttgg aatatggaac tgtccgcaac atataggtcg gcttcacaaa taaaacagtc
     6361 ctctttcccc attattggtg ctacagcatg tatagaagat atattgttaa tcgtccgaaa
     6421 ctccgtattc tctatcaaaa aaagattttc atatttttgg agaagaaaat caaactgttc
     6481 ttttagatat ccgacaacaa catagatatg tttaacacca cgttccacca gcccatcaat
     6541 aatagtttca atcatgggtt taccaaaaac cttaacgagt ggtttaggta ttttttctgt
     6601 tagaggagcc attctctttc ccattcctgc tgccatcaaa attgcaattt cattagttcc
     6661 tttgttctta ttctccagca tagttatttc ctttatttca atttcgttct tactatttaa
     6721 agaattgata ttatttcttt cgtaatagca tattctcaca tccgctcttc ttattgaagt
     6781 gtctttaaat aatccatgaa aatctccttg ttctccaaag ctgttgttgt tggacgtccg
     6841 aggttctctc tcgcagcaat tgaacatttc acttcaatta agctcaattc ccttctgttc
     6901 ttagctaatt caagctctct gtctaaatca tcataattat ctacacaaac tgcattagga
     6961 tatccgcaac cctttgcaat tgctacaaag tcaattctac ttgcaaccgt tggcataccg
     7021 ccaaccgatt catgtgcttc attattgata acaatatgaa ctatattcct tggtttgttt
     7081 gctccaatta ctgccattga acccatgtgc atgagaacag caccatcacc gtcaatacac
     7141 catatccgct gtttcggttt gtttattgct ataccgagag caatagaaga actgtgcccc
     7201 atagagccta cagttaaaaa atcgaactta tggctttgtc catttgccac tcttgcttca
     7261 aacagttcgc ggctggcttt tcctgttgta ctgacaatag ggtctttctc acttatcttt
     7321 aaaatatgtt gaattatttt ttcacgcacc attttattat tgtttgtgta ctcaacattc
     7381 ggagcataag ttagggctcc tttccgaatc acaaaggcaa cattcctacc ttgatttaag
     7441 atgcttctga actcagccat cttttcttca acttcatcat ctgaagtatc tttactaata
     7501 atgaaatttg cgattcccat atgattcagt aatttaatgg taacttcacc ttgaaagata
     7561 tgttgcggct catcatgaat accaggttct ccacgccatc caatcacaaa gacaaccggg
     7621 atagcgtaaa ctttatcatt taataagctt gctaccgggt tgattatatt gccttccccg
     7681 ctgttctgaa gataaacaac agggattttt cccgttgcca aatgataacc ggctgctaaa
     7741 gctgtacaat taccttcatt tgcagctatg atatggtgat gcggatcaat accgtatttt
     7801 gcaatcaaac aattacacag tgccttcaac tgtgaatctg gaactccggt ataaaagtcc
     7861 gatccgataa tctcaattag tttttcaact ttcatttatc gttatcctct cctcattgtc
     7921 ctcacagttc attaatcagg gtgatgatat tattaatcgg catcagtctg tcatcaactt
     7981 cttttgcacg atgatatttc agaatatctt cagcagtctg ctgcattgca ggaaatgcac
     8041 ttcgtgtcaa ttgattagca taaataacaa tgttaacacc atgcttaatc aattcttcct
     8101 ctgtaatagt gttaaaacta gaaggaacaa caacaattgg tgtgacttta tcttcttctc
     8161 taaatctatc gcagaactct aatatctctg caggatcttt ctttcgacta tgaatcatta
     8221 taccgtcagc accggctcta acaaaagctc tagacctttt cagtgcatct tccataccct
     8281 tttctaaaat cagactctca attcttgcga taatcatgaa atcatcagta agttgtgcct
     8341 tcttccctgc agcgattttt tcacaaaact cttcaatagt cgcctgagtc tgctcaacct
     8401 ctgtgccaaa cagactattt ttcttaagtc cttttttgtc ttcaatgata actgcagaca
     8461 cacccatgcg ctcaagagtt cttactgtat aaacaaagtg ttctgtaagt ccaccggtat
     8521 caccatcaaa gattataggc tttgtagtca cctccataat gtcatcaata gtacgaaaac
     8581 gactggtcat gtccaccaat tcaatatcag gtttcccctt tgataaacta tcacacaaac
     8641 tggatatcca cattgcgtcg aactgatcca gttttccatc atgctcaata actgtttttt
     8701 ctacaatcag tcctgtcaat ccgctatgag cttccagtac tttcactaca ggagctatgg
     8761 aaataagttg gcgaagtcgt tttctccgat actccggcat tgcaagtttt tcgcggagtt
     8821 gtacatcaat ctttttaact ttctcattac gagtataggg aatatcaacc agttcaccac
     8881 cataaacttt cagtagttct tcaacattat cacggatggc acgttccggg ccattcaacc
     8941 aattatcccc atgaacaaca taatccggct ggattattgg tataacatcg tcatatagca
     9001 tattgttctg aattacaacc tcttccacac catcaatgtc tttgtagagt ttaatgcgct
     9061 cttcttgact tattgttggg aatttgttgt accttattaa agcttcatca gacaagcagc
     9121 ctactataac tttccctctt tttttagcct cttttatgat attgagatgt ccgtcatgaa
     9181 ttacatctgt gcagaagcaa gtataaactg ttttcatcat ctatctcctt caatccttat
     9241 agactacagg tgtcttaact ccactcatag caagcgcctc tttgaacacc acatagaaat
     9301 cttcaatatc cgcttcatca atagctccga gagcacagag acgaaaggta tttgtggtgc
     9361 taatcttgcc tggatatatg gtgaaaccac gctcatagca gtagtcatgt atcttctcaa
     9421 aactccaatt cgaatcatcc ggataaatag ctgaagaaac aagcccggct ctccattcag
     9481 gcttgataac ttgtctgaaa cctaattcat ctaaaccttt gttaattgca ttaaacaccc
     9541 gggtatgacg tgcccacttt ccttcttcgc cttcagccca gtattctttt agtgcctgaa
     9601 tggtagcata gatagtttgt accggaggag taaaatgcat ttcacctgtg cgctcaaagt
     9661 aatcatactg ttgaaaaaga ttacagtaat aagaacgctt cggataatcc ttactttttt
     9721 ctataattgc ccgattaccc actacaaaac taagtccggt gaaagccatc aatcccttct
     9781 gtgcacttgc catgcagaaa tcgatgttat ccttttcaat gtcaatcgga cgcatagcat
     9841 aagtagatgt agtatcaacc gtgaaaattg cgccatactt atgagcaaga gcaccaatct
     9901 ctctaatagg attgagtagt cctgtaccgg tctcattatg ggtagtatgt actaaagcaa
     9961 tatctttatt atttttcaat atttcctcaa ctttttccat atcaggttgc tggtctacag
    10021 ggaactgaag atcaatgtga ggtaacccat aatatttaca gatttctaca gctcgagtac
    10081 tgtatgcacc gttgtttaca acgaaaactt tcttcccttt cggtaaaagg ctatttataa
    10141 caacatcaat attaatcgta ccgcttccgc agaataatac acttgtatac ttttccaaat
    10201 ctccatgaac aattctaact aaatcttctc tcaatccttt cattagacca gcaaattctt
    10261 tttcacgagg acatatatcc ggaacaactt gtgcatactt aacagtatcc gttgtcgtgg
    10321 acggtccggg atttaataat atgtttcttt ttatggcttc catataatct cctatgttat
    10381 tcatatttca ttttttattt tattctttta tagcaatatt gtctatttaa taatctaaat
    10441 atataaagca cttgattatt ctgtctatct tttttcatga tagaagcaca aatatacttt
    10501 ttatcatctc tctattcttc atttattctt ataacttgaa tatctaataa ccatgtaaca
    10561 gattaatact ccctattttt cagtctttag tggaatatac tctagtcttt ataatacaac
    10621 ctactttgct ttaactccat acttgcggga gcgaacactt atcattttca acattttttc
    10681 tgtattttct ccaaataagg gctcactgtt aaccatctgg ggaaatcatg cagattgatg
    10741 tgtggtattc acctcagtca atataatatt acctttctat caatactcaa atcccatgat
    10801 agaattccta aatatggcat ttgttcatgt aggtgtctag caaccttcct tactttatct
    10861 ccaacttctg gaataagata attttcaaaa gaaaccctga atctggatgc ttatcatatc
    10921 gttcaccgga ctcagaaaaa atatactttt tgagtgttcc gtccatattt acacatacag
    10981 atatccctcc atattgaata ttatccctat cagcatttga tgttccaagg tttaaagcaa
    11041 tcggatagac aaagacttgg tcttccaaaa tgtaggtaat aactcagaat gtatttaacg
    11101 aggatcaatt tagagaatat aatctctgat gttattaaac aaactcctag acaatgtaat
    11161 tgttcccaaa agaggccaat agtttaactg aattgtcagg tcctgactta acaattttga
    11221 catctctcct actactaata gcaacagtct tttttattac atacttttct tctcttctta
    11281 aaaattgttc accaatctct tgagaaacaa tgtgattatc tgaatctctt aaaaccccat
    11341 ttatggagga aataatagtt ttaggaatgt gtcctcctgt atttccaaaa aataccacta
    11401 ttaaatatct atcatctaaa cattctgcta tcctgcaaga atttgtttag gctccaattt
    11461 tgttgaaaac agaatctcag gaaaataatt ttcacaaaaa atacctgtat aactctaata
    11521 tagtctatgc tattcgtatc gaactctttc cgtaattctc caaaaaaatc tatcaatatt
    11581 atttttttgt ttggatgaat attctgtgta aagagtgtat ttctataaaa tcacagcata
    11641 tattcagata aatcagtgca gccatttcag aaaacatgta tagtatataa ccacaccata
    11701 ataattctta ttacttccta ttaaaacatc ggggtgaaaa cattcttata taattttctg
    11761 ctcaaaaatc ttttagtttc ttattgtaat tcctcaatat atttcatttt atataaatat
    11821 gtaatataaa gatgtcttct acattattta cttttaaaaa aattgtactg ttgaccagaa
    11881 tattctcctt tgcctactct ttttatagca tatctacttt acagttttac taccaataaa
    11941 aacattaaca caaagaagaa tacagtgaac agatgtcatc ctccgaaata tacacaggat
    12001 tattcctcaa tctttcaata ttcacagatt tcacaagctg taaaaaatct gattttttaa
    12061 caatcggact tttaatgttg tttttttcta taataccttc aattttacct attgctgctt
    12121 ttgggttgtc tacaccaaaa cagtttgcta tagaaaagaa tacattgttt agatactcta
    12181 tacctctttt gtcagtgcat ttatcaatat tgtctaccat atacccccat atctttggca
    12241 acaaaacagc aacagaacaa ccgtgcggta tgttataaag tgttgtcagt ttataactca
    12301 ttgcatgagt agctgttgtt ttagcaatat ttattgctct tccgcttaaa ttagcagcaa
    12361 gtaatattct atcagatgct tctttatcat cggttgtaag ataattatcc atattactga
    12421 ctatcaacat taaagcttct tttgaatact ctatactctg ttctgtagaa ttcaccgacc
    12481 accaagcttc tattgactgg cacatggcat ccatcatcgt acaaacctta tgtttcatag
    12541 gaagttgtga taaataattg ctgtttaata ttgcatactg aggtaatatg atgtcactct
    12601 ccacagattt cttttcatta tctacataca ctacagcaaa ttttgtagat tcactacctg
    12661 aacctgctgt tgtaggaata gcaataattg tatcatcgct atctatgttg atcttcttta
    12721 gataatcatc agggttatct atatttgaga atatcttgat acactttgct acgtcaatag
    12781 agcttcctcc acctacacat atgatacagt tacttcccga tactttatac ttttctactc
    12841 ctctacagat atcctctaat ttaggatttg aagtatattc gttaaatatt cctaccaacc
    12901 tgaaatcctt aatgaaaaaa tcataaatgt catgtttttt aattgatttg ccacatacaa
    12961 gaaaatactt agtgacattt aaatccttta atatcttttc tagttcaata atagtgtttt
    13021 ctgtccggat aattctttgc atacttatca ctcctcaggt attaatgaaa taatatcctt
    13081 tatcggcata cacatcttat cagcttcata tgctctgtga ttttcaagaa ttgtcattgc
    13141 tactttttgc atagcaggaa atgaacttct tgttaagtga ttagcatata taactatatt
    13201 tacacccttt ttttccaact cttcttctgt tactgtgtta tatgaagtag gaacaacaac
    13261 aataggtgtg tttttgtctt tctttctaaa aagttcaacg aactcaaata cttcatccgg
    13321 ttctttttta cgactatgta tcataattcc atcagctccg gcattaacat atgcaaaagc
    13381 tcttgatagc gcatcatcca ttcctttgtc cagaataagg gattctattc tggcaattat
    13441 cataaattcc tttgtctttt gagcattttt gccggctttg attttagtgc agaaattttc
    13501 tatatcatct tgagtttgta ttacatctga accgaacagc gaatttcttt ttaaacctgt
    13561 tttgtcttca ataataatag cagacactcc tactctctcc agtgtcctga cattatatat
    13621 gaagtgttcc actaagccac ctgtatctcc gtctaaaata ataggttttg tagtcacttc
    13681 cataatatca tctattgttc ttagtctcga agacatgtca acaagctcaa tatccggttt
    13741 gccttttgat gttgaatcgc ataatgatga aacccacata gcatcaaact gatatgcctt
    13801 gccatcctga tataccttgg tattctctgc tattaatcct gttattccat tgtgagcctc
    13861 aatagctgtt actaaatgtt tcactgataa taacttgcga agtctacccc tccttatatc
    13921 cggtattgaa agatgctctc tggctgaccg ttccaattca tcatatcttt tgtcctttga
    13981 atatggaaat tccactaatt ttccaccata agtagataga atctcaacta cttcatctct
    14041 tataggtttt tgaaatcctt ctttccagtc atccccatga acaacatatt caggcttgta
    14101 tttctctaaa ttcttcctat aacttaattc ttcttgaagc acaactctat ttacacccac
    14161 tatattctca aataatgttt tcctttcttc aaatggcatt aaaggatatc ttttaaaatt
    14221 tgctattgct tcatcggaca atactccgat agttaaccga ccaagcctgc ttgctttctt
    14281 taagatagcc atgtgaccgc tatgcaacat atcagtagaa aaacacatgt atacatccct
    14341 attcatgact gcttttaatc tcttagttat aaccaaaaga tcctcaggag tatcaatttc
    14401 gttacaaagt tcattcttaa catccaaagg ttttattaaa cacttctcgg aaacctcatt
    14461 aaatgcattt tcagcataac aattagtttg accattttca cagaaattaa taatcttatc
    14521 taaccaaact aaccagtccc tcctcttaag cttatacaac ggttgtgctg aataagcatt
    14581 ttcaaaaaca ttaaccgcaa ctgtgctgat atagccatct cttaactgta ctttaaagtc
    14641 tttctcaggt aagcttactg tagaactgat tgtcatacaa ctaccattaa aatccattac
    14701 tttatccaga acagaattct caaagactaa atctccatgc attaaaagaa tgtcgtcatt
    14761 taaatattct cttgcacagt acattgagta tatataattg gtcttatcat ataccgggtt
    14821 tttaacaaat gtatatttta gcgataaatc caatgattca cagtatttta caagaacttc
    14881 atcaaataag cctgtggtga taataacatc ctcaatacct acatcaacta actgcttaag
    14941 ctgtctacta agaatagtat ctgtattagt tatttctgtc atgcatttag gatgttcaga
    15001 tgtcaatact cccatcctgc gtcctaaacc tgaatttagt ataagggctt tcatttattc
    15061 acactcctta aggtattgca taaaatcttc tttattctct acaggtgatt ttgttggtct
    15121 acccaaatta ctgcgagatc ctattgctgt tttcacttca aaaaatacaa gtttattcat
    15181 ttttttaact ctgtttaatt ctatttctaa atcagcataa ttatcaacac tgattgtata
    15241 ctcatatcca cacccttgtg ctatctttac aaaatctatt tgccccccaa cagtcggcat
    15301 acctcctaca ctttcatgcg agttgttatt tattacaata tgaatcagat tgctaggttt
    15361 attagatcct attttagcca ttgaacccat atgcattatc gctgctccat cgccatcaat
    15421 gcaccatatt ttagttttct ttttttgtag tgcaatacca aaggcaatag aagaacagtg
    15481 tcccattgaa ccaactgtta agaaatcata tttatgagac tg
//
LOCUS       DAOSOM010000040        14997 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_4010_length_14997_cov_5.891715, whole genome shotgun sequence.
ACCESSION   DAOSOM010000040 DAOSOM010000000
VERSION     DAOSOM010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 14997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14997
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4010_length_14997_cov_5.891715"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..624)
                     /locus_tag="PLF14_08255"
     CDS             complement(148..624)
                     /locus_tag="PLF14_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71311.1"
                     /translation="MKYVTWIILIVFVAVLISVGFLIASRVDYFMYEKQVVSFVAKGI
                     QDGAIVRYNGKSVLVNKYNFEVMCGKLLTITEREKIHKVKEYDRDREIIIEVDDRNYV
                     VIMPLERSKAVYMETVLDGKRRYFYVSDKYRLHERVITYSRPEGFYGPNTLLDDSK"
     gene            complement(629..880)
                     /locus_tag="PLF14_08260"
     CDS             complement(629..880)
                     /locus_tag="PLF14_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71312.1"
                     /translation="MFKKKEYDDDDGRVIAPMNVEGMPWFQKGVGPQNANNEKATPLT
                     RKEMFSMLLNGALAGLTVALVLGFAAFLYILFCIHVWLR"
     gene            complement(883..1902)
                     /locus_tag="PLF14_08265"
     CDS             complement(883..1902)
                     /locus_tag="PLF14_08265"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007157646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HOT71313.1"
                     /translation="MANKRLKEKTFEPITPDKKYLLQVNALKTYFPIKAGVFSRVKGY
                     VKAVDGVSFNIKKGQTMGLVGESGCGKTTVGRTILKLTPHTDGEVYFDGQAIFDLTKK
                     EIIPLRPKMQIIFQDPYSSLSPRLPVGEIIGEAVREHKLVPEDKYDDYITSIMNSCGL
                     QAYHKDRYPHEFSGGQRQRICIARALALNPQFVVCDEPVSALDVSIQAQIINLLMDLQ
                     EKYGLTYLFISHDLSVVEHISDTIGVMYLGSLVETGSKKDIFKDPLHPYTQALFSAIP
                     VPDPDYKMKRIILEGSIPSPANPPSGCKFHTRCRHCFERCKKEDPKPKDVGNGHVVCC
                     HLYDK"
     gene            complement(1892..3067)
                     /locus_tag="PLF14_08270"
     CDS             complement(1892..3067)
                     /locus_tag="PLF14_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT71314.1"
                     /translation="MKLGKKFDKKIKSLNYLSAKESRAISRRNRKITDQLEKKKNRKN
                     VPVEEFTTKMRDENNVLEIDNLHTYFFTDLGTVKAVAGASFDVPKGKTVGVVGESGCG
                     KSVTSLSIMQLVQRPQGQIVDGEIRFNNGDVAYDIAKAPTEFMQTIRGNMISMIFQEP
                     MTSLNPVFRIGMQVDEVITLHNPDMSKEDVAKRTVELLDLVGIANAEGVYKMYPHELS
                     GGMRQRVMIAMALACSPDLIIADEPTTALDVTIQAQILDLLRTLKDKINSSIMLITHD
                     LGVIAEMADLVVVMYAGKVVEKGTADDIFKNAKHPYTVGLMESKPVINKDVDRLYSIP
                     GKVPNPINMPDYCYFKDRCEKCVAACSGHYPKEIKLSDTHYVSCYLYVDEEVKNNGK"
     gene            complement(3076..4908)
                     /locus_tag="PLF14_08275"
     CDS             complement(3076..4908)
                     /locus_tag="PLF14_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007157648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT71315.1"
                     /translation="MSRNNLPYDGGPENENRIDYIKEDDFTIRSSNIDEHISQSDSLD
                     DEQRVKMLSPTMLVAKRFVRNRLAIIGLIIILAMFVFSFVGGWLHPYPETKIFYKKEV
                     TSSQHATATINQNWYEEVKEGFEFTSINKAAFALALKDGKNIFTTKIDGKDVQFAIKE
                     LSADAVVLSETKRIGRIDIILGDYIFNQESDFVFSTAFKKAVLKAIDSKNSTIEFDGK
                     EYFLELSKRECVIYTGQEQVVLTKNIYDMYEEGTRADFEIKYAAETALKAHVDTFKVN
                     NKNYFIDYHEDTCTIYEVSGSEKIPFANISKLIVTPISADVFLSIDFKNAVEHAMYNN
                     ISSFTYTDDSGETVEYLVERHDLQWVIRTDKMTDLIDTYAFPSKEHPLGTDGSGMDIL
                     VRLMYGGRVSLTIGFVVVLIEIIIGVIIGGLSGYFGGWVDTLLMRLVEIFNCIPTMPI
                     YIILGAALDTMKLDPSVRIYLLMIILGIFGWPGIARVVRGQILSLREQEFMIANEATG
                     LSVYRRIFRHLVPNVIPQLIVFATMSLGGIILTEATLSFLNLGVKYPYASWGNIVNSV
                     NDSYVLQNYWFVWIPAGMLILITVLGFNFVGDGLRDAFDPKMKR"
     gene            complement(4924..5913)
                     /locus_tag="PLF14_08280"
     CDS             complement(4924..5913)
                     /locus_tag="PLF14_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT71316.1"
                     /translation="MWKFLVRRILLSIVILFFVSLIAYTIIRALPTSYVEGIARERAS
                     LPGGKSYTEWLDQLNKIYHLDGGVIEGFFGWFKDSLLKGEFGDSWKFNVPVVQKFKEV
                     IWDSFFLGLISLILELVIAIPLGILAARKQYSKTDYAVSVFVLIGISLPTFFFATLLK
                     LGFAYKLGWFEPVGKVSRYYDQLTPVGQFFDVASHFVLPVVTLTIVSLGFLMRYTRTN
                     MLEVLNSDYIRTARAKGLSEKTVINKHAFRNTLIPIVTIVGGTLPGLFSGAMITEQLF
                     SIPGIGYTAYQAMIVGDIPFSMFYLVFLATLTLIGTLISDILYAVVDPRVRVN"
     gene            complement(6023..8461)
                     /locus_tag="PLF14_08285"
     CDS             complement(6023..8461)
                     /locus_tag="PLF14_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT71317.1"
                     /translation="MKFKTKSILSIMLVVILCIGMLASCTPKAKKGEATILTPLVVAY
                     DPFSGKFSPFFADTAYDNDVAGMTQVGLMTTDRVGGVIFNAIKGETVNYNGTDYLYTG
                     VADLSVNYDEKSDTTTYRATIRKDVAFSDGHIMDIDDVIFTYYVYCDPSYTGSTTLSS
                     YNILGLRNYQTQTTDEVYDKYVEMANAIYAAGEDHAWSASDTWTKEQQDGFWTLLNNE
                     WKADVDSIVKYVYAKYLPYADAGYFTFSSAEIAASEGLQVAYGMRMWGFAKPDGKTLI
                     TAVTGKKFDMAAGEYPTVDDYFAEVKAAYEGNPEAYCDAGESPNSVDVLGNAKTKFIL
                     QFGSKDEAMGKEGIKSISGIKRINNRTVEVVTKGYEAPAIYAILGVTITPMHYYGDES
                     LYDYKNGSYGFPFGDLSMIQAKTSNPMGAGPYKFVKYEDKVVYFEANEYYYKGCPKVK
                     YVQFKEIGESDKISSVASGAADVANPSGSKVKFNEIRSLNSNGQLDGDKITTNSVNNL
                     GYGYIGINADTVRVGNDSASTASKNLRKGFATLLAVYRDVAVNSYYGDAASVINYPIS
                     NTSWAAPQKSDADYKVAFSVDVDGNPIYTADMDAEAKYAAALNAAIGYFKAAGYTWKD
                     STKMFTAAPDGASLTYEALIPGDGQGDHPNFQVLTNTAEALKKIGITLEVNDLADSSV
                     LWERLDAGTQELWTAAWGSTIDPDMYQVYHSSGIVGLGGSDSNHYHIADAELDNLIVE
                     ARKSDDQTYRKSVYKACLDIIIDWAVEVPSYQRQNIVIFSTERVNIDTVTPDITTFWG
                     WMNDIELLEVYEAQ"
     gene            complement(8681..10030)
                     /gene="glmM"
                     /locus_tag="PLF14_08290"
     CDS             complement(8681..10030)
                     /gene="glmM"
                     /locus_tag="PLF14_08290"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOT71318.1"
                     /translation="MGKIFGTDGARGIANKELTVSLAFDLGKAGAYVLAGENRHKPKI
                     LIGADTRISSDMLMSATEAGLCAMGADVIRAGVITTPAVAYLTKLYGCDAGIVISASH
                     NSYEYNGIKFFNRDGFKLPDETEEHIENFITDKIEVSVKTHNEIGRIYMNCHCVEAYI
                     DILINNCSTDLSELNILLDCANGAASAIAPEVFRRAGCNVSHIHSSPDGLNINKDCGS
                     THINSLAEHVKKGGFDVGFAYDGDADRLIAVSERGEVIDGDVLMSVLALYLRSKGLLY
                     KDTLVATEMSNLGITVFGKSNSIEIIRTKVGDRYVVEEMRKGGYSIGGEQSGHIVLLP
                     YTTTGDGILASLKIAEILAENNIKASSFFEIMKPLPQVLVNVRVDNEMKGKLKDDMEI
                     QALSEKVSKELGGNGRVMVRASGTEPVIRVMIEGEDFTFIENRANMIADMIRLKLGK"
     gene            complement(10091..11596)
                     /locus_tag="PLF14_08295"
     CDS             complement(10091..11596)
                     /locus_tag="PLF14_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71319.1"
                     /translation="MWNKIKKNLFFKILAVFIAIIIWFIVSVNNNPIEVKTVSVPIYV
                     QNERNLALRNLKIVNNFDHFIDVQVKGAATEVNKVSASDFTAYVDYVEIINESITELT
                     VKGLNYSGNANITYSCKEEDATKAIRVERLISSEFPVTVNFNGEPAEGYELVTYTVIP
                     NIQSVNDVTSMITGISGIRVDIDLDDTTQSFTVRKACDVYDKSMHIMNEYTNTITVDV
                     SVTIGKVVNVVSKLTGTANLGGNHMYISDSPEYNRAIIVGDYAIIKDINTVYTKPVDI
                     SGKTQSFTTEAELDIPSNIKVYTLNYVLNLNNKVLLNVVIEQLTTREFEFGIEELRVR
                     NKNILKEYTIREEMFNLTLKGRESTLAEITKEQIVPYIDVLDMEDGNAYRSVNILSLG
                     NNITLSESPDLNIYVETIATYSIDSNRISLTNVDSDFIYSIQNETFNMKLVGLSADIS
                     DTDISEMKFYVNVQDLKEGVHMVDVIVKDVNLPENVRFYEDMQVAVEITVP"
     gene            complement(11586..12461)
                     /gene="cdaA"
                     /locus_tag="PLF14_08300"
     CDS             complement(11586..12461)
                     /gene="cdaA"
                     /locus_tag="PLF14_08300"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOT71320.1"
                     /translation="MFNQIDWVEQIKYFSLELSSPLTVISAIIDIAIVSVLIYKLMIF
                     VKEIRAWSLLKGVLVIVFVAIVSNLLGLQIMTFLFNNIITFAALSVVVLFQPELRKVL
                     ERLGTSEITHFFTNTGSEETLMITTVVDEIVKACESMADRYTGALIVIEKSTKIGEFI
                     NTGIQLNADVSKEILENIFVPKTPLHDGAVIIRKDKIVAAACLLPLTDNPNLSTELGM
                     RHRGGLGITEVSDCIAVIVSEESGKISYASNGGLVRNLSPDSLRKALLMNLVREKKEK
                     NRIGKIIRKGGKNVE"
     gene            complement(12574..14208)
                     /locus_tag="PLF14_08305"
     CDS             complement(12574..14208)
                     /locus_tag="PLF14_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HOT71321.1"
                     /translation="MPITEILEKNARLYPDDVSLVEINPELQEKRQVTWKEFELIETN
                     PKEHYRKEITWKEFDDKANRLANFLIANGITKGKKVAILLMNCIEWLPIYFGILKTGA
                     LVVPLNYRYTAEEIRYCLNLSESNALIFGYEFISRIESIKEQISNIKPLIYTGKDCPK
                     FAVSYEAETEKYPAVSPKVEISDADDAAMYFSSGTTGFPKAILHTHSSLVSACITENI
                     HHGQTRQDNFLCIPPLYHTGAKMHWFGSFLVGSKAVLLRGVKPKWILKAVSEEKITIV
                     WLLVPWAQDILDCLERGELDLKDYKLDQWRLMHIGAQPVPPSLIRRWKKYFPNHAYDT
                     NYGLSESIGPGCVHLGLENIHKVGAIGLPGYGWSTRIVDEKGCDVVNGEVGELLVKGP
                     GVMKCYYGDKAATDAVLKDGWLYTGDMAKQDSDGFIYLVDRKKDVIITGGENLYPVQI
                     EDFLRGHDAIKDVAVIGLPDKRVGEIAAAIVELKEGYECTKEELDTFCLGLPRYKRPR
                     AIIFDKVPRNPTGKIEKPKIREKYCKMSVVELQIST"
     gene            complement(14323..14775)
                     /locus_tag="PLF14_08310"
     CDS             complement(14323..14775)
                     /locus_tag="PLF14_08310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HOT71322.1"
                     /translation="MISGNIDGTGVPACMDDYLMRAMEIMKGLNTESPSGKVAVDGDD
                     FYYTVMEVEVKPVEEIRFEMHRQYIDIHYVIEGNERIAVANGNMLKVSQPYSEEKDCA
                     LCYDGQVMSIIALQAQDFCVIYPYEAHKPGGMVNDFCKIKKAVFKIKV"
BASE COUNT         4064 a         3540 c         2647 g         4746 t
ORIGIN      
        1 agtgtagtct ttccgcttcc cgttcctccc gctattccga ttaccagtat gtccatgata
       61 aacccctctt ttagtttata ctatgcctat tatatatcat atatatcaat ttctaccgat
      121 gtattatata gctttgttgt aaacaactca ttttgagtcg tcaagcaatg tattggggcc
      181 gtaaaaacct tccggtcggc tgtaagttat taccctttcg tgtaacctgt atttgtccga
      241 tacatagaaa tatcgccttt ttccgtccag cacggtttcc atatagacgg ccttgctgcg
      301 ttcaagaggc attatgacca cataatttct gtcatccact tcaattatta tttctctgtc
      361 ccgatcatac tctttaacct tatgaatctt ctctctttcg gttattgtca agagtttacc
      421 gcacatgact tcaaaattat atttgttaac cagtaccgat ttgccgttgt aacgcacaat
      481 agcaccgtct tgaatgcctt tggcaacaaa agacacaacc tgcttttcat acataaaata
      541 gtccactctg gaggctatga ggaatcccac tgatatcagg accgctacaa acactatcag
      601 aattatccat gtaacgtatt tcatgatatc accttaacca tacatgtatg cagaataaaa
      661 tatacaaaaa tgcggcaaac cctaaaacaa gcgctaccgt aagaccggct aaagcgccgt
      721 taagaagcat cgagaacatt tcctttcttg tcaaaggagt agctttttca ttattcgcat
      781 tctgcggtcc gacgcctttc tgaaaccagg gcatcccttc cacattcatg ggagcgatta
      841 ccctgccgtc atcatcgtcg tattctttct tcttaaacat ctctatttat cgtacagatg
      901 gcagcacacc acatgtccgt tacctacatc cttaggtttg ggatcttctt ttttacatct
      961 ttcaaagcag tgtctgcatc tggtatggaa cttgcatccc gaaggaggat ttgcaggcga
     1021 aggaatactt ccttccagta ttatcctctt catcttgtaa tcgggatcgg gtaccggtat
     1081 ggcactgaat aatgcctgtg tatagggatg aagcggatcc ttgaatatat ctttcttaga
     1141 tcctgtctcc accaaagatc ctaagtacat tacccctatc gtatcggata tatgctcaac
     1201 aaccgacagg tcatgagaaa taaacagata tgtcagtccg tatttttcct gaagatccat
     1261 aagaagattt ataatctgtg cctgaatgga aacgtccaga gcggaaaccg gctcgtcaca
     1321 caccacaaac tgcggattga gtgctaacgc tctggctata catattcttt gtctctgacc
     1381 gccggaaaac tcatgaggat atcggtcttt atgatatgcc tgcagtccgc aggagttcat
     1441 tatcgatgtg atataatcat catatttatc ttccggtacc agtttatgct ccctcacggc
     1501 ctcccctatt atctcgccta cgggaagacg gggtgaaaga ctggaataag ggtcctggaa
     1561 tattatctgc atcttgggac gcaagggaat tatttctttc ttggttaagt caaatattgc
     1621 ctgaccgtca aaatatactt caccgtcggt atgaggcgtc agcttcagaa tggtacgtcc
     1681 tactgttgtt ttaccgcatc ccgattctcc aaccagcccc atagtctgac cttttttaat
     1741 attgaatgaa actccgtcaa cagctttaac atacccttta acgcgggaaa atacacctgc
     1801 cttaatcggg aaatatgttt ttaaggcatt gacctggagt aaatattttt tatcgggagt
     1861 tattggctcg aatgtctttt ccttcaatct tttatttgcc attattcttt acctcctcgt
     1921 caacgtacag atagcaactt acataatgag tatcggaaag tttaatctcc ttcggataat
     1981 gacctgaaca tgccgcaacg catttttcgc atctgtcttt gaaataacag tagtcgggca
     2041 tgtttatggg attgggaact ttaccgggta tgctgtacag tctgtccacg tccttattaa
     2101 taacgggttt gctttccata agtcctacgg tataggggtg cttggcattt ttgaatatgt
     2161 catcagccgt tcctttttcc accacttttc cggcatacat taccacaacc aaatccgcca
     2221 tctcggcaat aacgcccaaa tcatgtgtta tgagcattat gctgctgtta atcttatcct
     2281 ttaatgttct taagaggtcc agaatctggg cctgtatggt aacatccaat gcggttgtgg
     2341 gctcgtcggc tattataagg tcgggactgc aagccagagc cattgctatc ataactcttt
     2401 gtctcatacc gcccgataat tcatgaggat acattttata tacgccttca gcgttggcta
     2461 tacctacaag atccagcaac tccaccgttc gtttggcaac atcttctttc gacatatcgg
     2521 gattatgaag agtaattacc tcatccacct gcatgcctat cctgaaaacg ggatttaagc
     2581 ttgtcatggg ttcctggaat atcatggata tcatgttacc gcgtattgtc tgcataaact
     2641 ctgtaggagc cttggcaata tcatatgcaa catctccgtt gttgaaccgg atttcaccgt
     2701 ccacaatctg tccctgaggg cgttgaacca gttgcattat ggataaactg gtaacggatt
     2761 ttccgcatcc cgattcgcct accacaccga cagtcttacc tttgggaaca tcaaatgaag
     2821 ctccggcaac cgctttaacc gtacctaaat cggtaaagaa gtatgtatgc aaattatcta
     2881 tctccaatac attgttctca tctctcatct ttgtggtgaa ctcttctacg ggaacattct
     2941 ttctgttctt ctttttctcc agttggtcgg taatctttct gtttcttctg gaaatagccc
     3001 gcgattcctt tgcggaaagg tagtttaagc tctttatttt tttatcgaat tttttaccaa
     3061 gtttcatttt ctcacctacc ttttcatctt aggatcaaat gcatccctaa gaccgtctcc
     3121 cacaaagtta aatcctaaaa cggttataag tatcagcatt cccgctggaa tccatacaaa
     3181 ccagtaattc tgcaatacat agctgtcgtt aacggagttt actatattac cccaggaagc
     3241 atacggatac ttgacaccga gattcaaaaa gctcaatgtc gcttcggtaa gtattattcc
     3301 gcccaagctc attgtcgcaa acactataag ttgcggaata acattgggca caagatgcct
     3361 gaatatcctt ctgtaaacag ataatccggt ggcttcattg gctatcataa actcctgttc
     3421 tctcaaggat aaaatctgac ctctgacaac ccttgctata cccggccatc cgaagatgcc
     3481 taaaataatc attaaaagat aaattcgcac actgggatcc agcttcatgg tatccaaagc
     3541 ggctcctaaa ataatataga taggcatggt gggtatacag ttgaatattt ccacaagcct
     3601 cataagtaag gtatccaccc agcctccgaa atatcccgat aaaccgccta taataactcc
     3661 tataattatt tctatcaaca caacgacaaa tcctatagtt aatgatactc tgccaccgta
     3721 cataagtctt accagtatat ccattcccga accgtcggtt cccagcggat gttctttact
     3781 ggggaaagca taagtatcaa tcagatcggt cattttatcc gtccttataa cccactgtaa
     3841 atcatgcctt tccacaagat attccaccgt ctctcctgaa tcatcggtat acgtgaaaga
     3901 ggaaatatta ttgtacatcg catgttccac ggcattttta aaatcaatag ataagaaaac
     3961 atcggcggaa ataggagtta cgataagttt ggatatattg gcaaacggta tcttttccga
     4021 acctgatact tcatatatcg tacaggtatc ttcatgatag tctataaagt aattcttatt
     4081 attcacttta aaggtatcaa catgtgcctt taaagccgtt tccgctgcat actttatctc
     4141 aaaatccgct cttgtacctt cttcatacat gtcatatatg ttcttggtta acacaacctg
     4201 ttcctggccg gtataaataa cacattctct tttcgaaagt tcaagaaagt actcttttcc
     4261 gtcaaattcg atagtgctat tcttactgtc aatagcttta agaacagcct tcttgaaagc
     4321 cgtggaaaac acaaaatcgc tttcttggtt aaaaatgtaa tcgccgagta ttatatcaat
     4381 cctgccgatt ctttttgttt cggaaagtac cactgcatcg gcagacagct cttttatcgc
     4441 gaattgcaca tccttgccgt ctattttagt ggtaaatata ttctttccgt ccttcagcgc
     4501 caatgcaaaa gccgccttat tgatggaggt aaactcaaaa ccttctttta cttcttcata
     4561 ccagttctga tttattgtag ctgttgcatg ttgcgaagaa gtaacttctt ttttatagaa
     4621 aatctttgtt tccggatagg gatgcaacca acctcccaca aaggaaaata caaacattgc
     4681 aagtattatg ataagtccta ttatcgcaag cctgtttctt acaaaccgct ttgctaccag
     4741 catggtggga gaaagcatct tgactctctg ttcatcgtct aaactgtccg attggcttat
     4801 atgttcatca atgtttgaag agcggattgt aaaatcatcc tcttttatgt aatcaatcct
     4861 attttcgttt tccggtccgc cgtcatacgg caaattattc ctgctcattt tccgcccccc
     4921 ttcctaattg actctgactc tcgggtccac tacggcatac aatatatcgg agataagagt
     4981 accgataagc gtaagtgtcg ctaagaatac cagataaaac atggaaaaag gtatatctcc
     5041 cactatcatt gcctgatatg ccgtgtaacc tattccgggt attgaaaaga gttgttccgt
     5101 tatcatcgca ccggaaaaaa gtcccggcaa ggttccgccg acaatggtta cgataggaat
     5161 cagggtgttc ctgaaggcat gtttattaat taccgttttt tccgaaagac cttttgctct
     5221 ggcagtcctt atataatcgg aattaagtac ttccaacata tttgttcttg tatatctcat
     5281 caaaaagcct aagcttacta ttgtcaaagt aactaccggc aacacaaaat gcgatgccac
     5341 atcgaaaaac tgtccgacag gtgtcagctg atcatagtac ctgctcactt ttcctaccgg
     5401 ttcaaaccat cctaatttgt aggcaaaacc gagttttaac agtgttgcaa agaaaaatgt
     5461 aggtaaagaa atacctatca gtacaaatac acttacggca tagtcggtct tgctgtactg
     5521 ctttcttgcc gctaaaatcc ccaaaggtat tgctataacc agttccagta tcaatgatat
     5581 aagtcccaag aaaaatgagt cccaaataac ttccttgaac ttctgcacca ccggaacatt
     5641 aaacttccat gaatccccga attcaccctt taacagtgaa tccttaaacc atccgaaaaa
     5701 tccctctatt actccgccgt ccaaatggta aatcttatta agctgatcca gccattcggt
     5761 ataacttttt ccgcccggaa gcgaagctcg ctctctggct attccctcaa cataagatgt
     5821 gggcagagca cgtataatcg tatatgctat cagggatacg aaaaaaagta tcactatact
     5881 taataatata cgtctaacca aaaacttcca cattaattta ctccttatca aaactgtgcc
     5941 taaatacgca gtttaaaata ttatacacat aaggaaagcc cgccgtcaac agcgggcttt
     6001 cctgtgttgc taaatattta cattattgtg cttcataaac ttccagaagt tcaatgtcat
     6061 tcatccagcc ccagaatgtt gtgatatcgg gtgtaaccgt atcaatatta actctttcgg
     6121 tactgaatat aacaatgttc tgtctctgat atgaaggtac ttctaccgcc cagtcgataa
     6181 tgatatcgag gcatgctttg tatacggact ttctgtatgt ctggtcatcg gacttacgag
     6241 cttcaacaat cagattatcc aattcggcat cggcaatgtg gtaatggttg gaatccgatc
     6301 ctcccaaacc tactattccg gagctgtgat atacctgata catatcagga tctattgttg
     6361 agccccatgc agctgtccac agttcctgtg tacctgcatc cagtctttcc cagagaactg
     6421 aagagtcagc caagtcgtta acttccaaag ttattcctat cttctttaga gcttccgctg
     6481 tatttgtaag aacctggaag ttaggatggt caccttgtcc gtcgccgggg atcagtgctt
     6541 catatgtcag gcttgcgccg tcgggagcag ccgtaaacat cttggttgaa tctttccatg
     6601 tgtaacctgc tgccttgaag tatccgatag ctgcatttaa tgctgctgcg tatttagcct
     6661 ctgcatccat atccgccgtg tagatgggat ttccgtctac atcaaccgag aatgcaactt
     6721 tatagtcggc atcggatttc tgaggagccg cccaggaagt attggaaata ggatagttga
     6781 taaccgatgc tgcatcacca tagtagctgt taacggcaac atcacgatat actgcaagca
     6841 aggtagcaaa accttttctc aggttcttgg atgccgtgga agcggaatca ttacctactc
     6901 ttaccgtatc ggcattaatt ccgatatatc cgtatcctaa gttgttaacg gaatttgtcg
     6961 taatcttatc gccgtcaagc tgtccgttgg agttaagcga acgaatctcg ttaaacttaa
     7021 ctttgctgcc gctcggattt gctacatcag ctgcaccgga agcaacggaa ctaatcttat
     7081 cgctttcgcc gatttccttg aattgaacgt atttaacctt cgggcatccc ttgtagtaat
     7141 attcgttagc ttcgaagtaa acaactttat cttcgtactt gacaaatttg taaggacctg
     7201 cgcccatagg attggatgtc tttgcctgaa tcattgaaag gtcgccaaac gggaatccgt
     7261 agctgccgtt cttataatca taaaggcttt cgtctccgta gtagtgcatc ggtgtgattg
     7321 ttacgccgag tatagcatat atagccggtg cttcatatcc ttttgttaca acttccactg
     7381 tacggttgtt tattctcttg ataccggaaa tgctcttgat tccttcttta cccattgctt
     7441 cgtcttttga accgaactga agtatgaatt tggttttggc attgcctaat acatcgacac
     7501 tgttaggaga ttcgccggca tcacaatatg cctcgggatt tccttcatat gccgctttta
     7561 cctctgcgaa gtagtcgtcc actgtcggat attctcctgc cgccatgtcg aatttctttc
     7621 ctgttacggc tgttataagg gtttttccgt ccggtttagc aaatccccac attctcattc
     7681 cgtatgctac ctgtaagcct tcggaagcgg ctatctccgc tgaagagaac gtaaagtatc
     7741 ctgcgtccgc atatggcaga tattttgcat atacatattt gactattgag tcgacatcgg
     7801 ctttccactc gttattgagc aatgtccaga atccgtcctg ctgctctttt gtccatgtgt
     7861 cggaagccga ccatgcatgg tcttcgcctg cagcgtaaat tgcattggcc atttcaacat
     7921 acttgtcata aacctcatca gtagtctgtg tctgatagtt tcttaatccc agtatgttgt
     7981 aagatgataa cgttgtggaa ccggtatatg aaggatcgca gtaaacgtaa taagtgaata
     8041 ttacgtcatc aatatccatt atatgtccgt ccgagaacgc aacatctttt ctgatggtcg
     8101 cacgataggt tgtggtatcg gatttttcgt cgtaattaac cgacaaatcg gcaacgccgg
     8161 tgtagagata atcggttccg ttgtagttaa ccgtctcacc cttgatagcg ttgaaaataa
     8221 caccgccaac cctgtcagtg gtcatcagtc cgacctgtgt cattccggct acgtcattgt
     8281 catatgccgt atccgcaaag aacgggctga acttgccgct gaaaggatca tatgcgacaa
     8341 ccagcggagt cagtatggta gcttcgcctt ttttagcttt aggcgtacag cttgctaaca
     8401 tacctatgca aagaatcact acaagcatga tgctgagtat gctctttgtt ttgaatttca
     8461 taattttacc tcctcgtagt aaataactct ttataaataa agttttagtc ataatatcat
     8521 gcacatgaaa aagtgtcaat atattttttc acattgagtt cacatttcag gctcaatttg
     8581 cataaaaatg actgattaat gctatttttc aggttttctg agtaaattca ggtatttcat
     8641 ctaatagaaa atgaataaat cggtttcaaa atattcatat ttatttgccg agctttaatc
     8701 ttatcatatc ggctatcata ttggctctgt tttctatgaa agtaaaatct tcaccttcta
     8761 tcattaccct tatcaccggt tccgttccgg aagccctgac cattaccctt ccgttaccgc
     8821 ccagttcctt ggaaactttc tctgaaagtg cctgaatttc catatcatcc ttcagtttgc
     8881 ccttcatttc attatccact ctcacattga caagcacctg gggaagaggc ttcataattt
     8941 caaagaaact gcttgcttta atattattct ccgcaagtat ctcggcaatt tttaacgatg
     9001 ccaggatacc gtcacccgtt gtggtgtacg gtaacagtac aatgtgtcca gactgttctc
     9061 ctccgatgga atatccgcct tttcgcatct cttccactac atatctgtct ccgacttttg
     9121 tccttataat ctctatggag ttgctcttgc cgaaaacggt aatacccaaa ttgctcattt
     9181 cggtagctac cagggtgtct ttgtacagca gtcctttgct ccttaagtac agagcaagta
     9241 cggacatcag cacatcaccg tcaattactt ctcccctttc cgataccgca ataagacgat
     9301 ccgcatctcc gtcataagca aaaccgacat caaaaccgcc ttttttaaca tgttccgcca
     9361 ggctgttgat atgagtggat ccgcagtctt tattgatgtt taaaccgtcg ggagaagagt
     9421 gaatgtgtga aacattacag ccggcccttc ggaatacttc cggtgctatt gcacttgcgg
     9481 caccgtttgc acaatccaaa agtatgttca attcggaaag atcggtactg caattgttta
     9541 taagaatatc tatatacgct tcgacacaat gacaattcat atatattctg cctatctcat
     9601 tatgtgtctt tacacttacc tcaattttat cggtaataaa attctctata tgctcttccg
     9661 tctcatctgg caatttaaac ccgtctctgt tgaagaattt tattccgtta tattcgtaag
     9721 aattgtgtga ggcggatatg acaatacccg catcgcaacc gtaaagtttt gtaagatatg
     9781 ctaccgcagg ggtggtaata actcccgccc ttattacatc ggctcccata gcacacaggc
     9841 ccgcttcggt agccgacata agcatatccg acgagattct ggtatcggcc cctattaata
     9901 tcttaggttt atggcggttt tcccccgcca gtacataagc accggctttt cccaaatcaa
     9961 aagcaaggct tacagtcaat tctttattgg ctatacccct tgctccgtcc gtaccgaata
    10021 tttttcccat agtacctcca cacctgaagt tcgtgttttt tactttagaa tcttaatatt
    10081 caattactcg ttacggtact gttatttcga ccgcaacctg catgtcctca taaaaccgca
    10141 cattctccgg aagattgacg tctttcacta tcacgtctac catatgcaca ccttctttaa
    10201 ggtcctgtac atttacataa aacttcattt cggaaatgtc ggtatcggaa atatcggcac
    10261 ttaaaccgac aagcttcatg ttaaacgtct cattctgtat ggaatatata aaatccgagt
    10321 ccacattggt cagcgaaatc ctgttgctgt ctattgaata ggttgctatt gtctcaacat
    10381 atatattcag atcaggtgat tcggaaagcg ttatattgtt gccgagcgac agtatgttta
    10441 cactgcggta tgcgtttccg tcttccatat ccagaacatc aatataaggt acaatctgtt
    10501 ctttggtaat ttcagccaat gtagactccc gtcctttgag tgtgaggttg aacatttcct
    10561 ctcttatcgt atattccttg agaatatttt tatttcttac tctaagttct tctataccga
    10621 actcgaactc tctggttgta agctgctcta ttacaacatt taaaagcacc ttattattca
    10681 aattcaatac ataattcaac gtataaacct ttatattcga gggaatatcc agctcggctt
    10741 ccgttgtaaa cgattgggtt tttccggata tatccacagg tttggtgtaa acggtattta
    10801 tgtctttaat tatcgcataa tcgccgacta taatagcccg gttatattcc ggcgaatcgc
    10861 ttatatacat gtgattgcct ccgaggttgg cagttcccgt aagtttgctt acaacattga
    10921 ctactttacc gatggttaca ctgacatcca cggtaatggt atttgtatat tcattcatga
    10981 tatgcatact cttgtcgtat acatcacaag cttttcttac cgtgaaagac tgtgtggtat
    11041 catccaaatc aatatctacc cgtataccgc ttatacccgt tatcatggat gtaacatcat
    11101 tgaccgactg tatattggga attacggtat aagtaacaag ttcatatcct tccgccggtt
    11161 ctccgttgaa atttaccgtt acgggaaatt ccgaggatat cagtcgctcc acccttattg
    11221 ccttggttgc atcctcttct ttacaggaat aagtaatatt cgcattgccg ctgtaattca
    11281 gtccttttac ggtaagttcc gttatcgatt cgtttattat ttccacataa tctacgtatg
    11341 ctgtaaaatc cgaagccgac actttgttta cctcggtagc ggcaccctta acctgaacat
    11401 caataaaatg atcaaaatta ttaacaattt taagatttct taaggcaaga ttcctttcgt
    11461 tctgtacata aatgggaacg gaaacggttt ttacttcaat gggattatta tttacggaaa
    11521 ctataaacca tataatgata gctataaaca ccgctaatat cttgaagaag agattttttt
    11581 taatcttatt ccacattctt tccgcccttc cttataatct tccctattct gttcttttct
    11641 ttcttctctc tgacaagatt cataagcaat gccttacgca aggaatcggg acttaagttt
    11701 cgcacaagtc ctccgtttga ggcataggaa attttcccgc tttcttccga cacgataacg
    11761 gctatacagt cggatacctc ggtaataccc aaacctcctc tgtgtctcat tcccagttcg
    11821 gtactcaaat tgggattatc ggtaaggggc aacagacagg ctgcggcaac aatcttatct
    11881 ttacgaataa ttacggcacc gtcgtgtaaa ggggttttag gaacaaatat attctccaaa
    11941 atctctttgg aaacatcggc attcaattgt atcccggtat tgataaactc acctattttt
    12001 gtggatttct ctattactat aagggcaccg gtatacctgt ccgccatact ttcgcatgcc
    12061 tttactatct catccactac agtggtaatc atcaatgttt cttccgatcc ggtattggtg
    12121 aagaaatgcg taatctcgct tgtacccagc cgttccaata ctttcctcag ttccggttga
    12181 aataagacaa caacggacaa tgcggcaaag gtaattatat tgttgaaaag gaaggtcata
    12241 atctgtaagc ccaacagatt gcttactatt gcaacaaaca caattaccag tacacctttt
    12301 aaaaggctcc atgctcttat ttcctttaca aaaatcatca gtttataaat aaggaccgaa
    12361 acaatcgcta tatcgattat tgcggatata acggtaagag gactggacaa ttccaaactg
    12421 aaatatttta tttgttctac ccaatctatc tgattaaaca ttgtactccc cgtgtcggtc
    12481 tttatacatt accgctctaa ttatatatca ttataatacg gtatgcatat attttaatgc
    12541 actttttata tacttaaaat gcaataaaac ggtttatgta ctgatctgta actccaccac
    12601 actcatttta cagtacttct cccgtatctt gggcttttca atcttaccgg tcggatttct
    12661 gggaaccttg tcaaaaatga tggctctggg tcttttatac ctcggtaatc ccagacaaaa
    12721 agtatccagc tcttctttgg tacattcata tccttctttg agttccacaa tggctgcggc
    12781 aatttcccct actctcttat cgggaagccc tatgaccgca acatccttta tcgcatcatg
    12841 acctcttaag aaatcttcta tctgaacggg ataaagattt tcacctccgg taataatgac
    12901 atcttttttc ctgtccacaa gatatatgaa tccgtcactg tcctgcttgg ccatatcacc
    12961 cgtatacagc catccgtctt tgagaacggc atcggtagcc gctttgtcac cgtagtaaca
    13021 tttcattact cccgggcctt ttaccagtaa ttcacccact tcaccgttta cgacatcgca
    13081 ccctttctca tccactatcc tggttgacca tccgtaaccc ggcagtccta ttgctccgac
    13141 tttatgtata ttctccagcc ctaaatgcac acatcccggc ccgatggatt cgctcagtcc
    13201 gtaattggtg tcataggcat gattcggaaa atatttcttc cagcgtctga taaggctggg
    13261 aggaaccggt tgtgcaccga tatgcatcag tctccactgg tccagcttat agtccttaag
    13321 atccaattct cctctttcca agcaatctaa gatatcctga gcccacggta caagaagcca
    13381 tacaatggtt attttctctt cgctcaccgc cttaagtatc cacttgggtt tcacgccccg
    13441 aagaaggacc gctttgctgc ctaccaggaa actgccgaac cagtgcattt tggctccggt
    13501 atggtataaa ggaggtatgc ataaaaaatt atcctgtctg gtctgtccgt gatgaatgtt
    13561 ctccgtaatg catgccgaca ccagactgga atgagtatgc agtatcgcct tgggaaatcc
    13621 ggtggttccc gatgaaaagt acattgcggc atcatcggca tcggatatct ccactttcgg
    13681 agaaacagcc gggtattttt ccgtctccgc ctcataactt acggcaaact tcggacagtc
    13741 tttaccggta tagattagcg gctttatatt actaatctgc tcctttatac tctctattct
    13801 gcttatgaac tcatagccga atataagggc gttgctttcc gacaaattca aacagtatct
    13861 tatttcttca gccgtatacc tgtaattcaa aggcacaacc aaagcaccgg ttttaagtat
    13921 accgaaatat atcggcaacc actctataca attcatcagg agaatggcaa cctttttccc
    13981 tttggtaatt ccgttagcga ttagaaaatt agccaatctg ttggctttgt catcaaattc
    14041 tttccatgtt atttctttcc tgtaatgttc tttgggattt gtttcgataa gttcgaattc
    14101 cttccatgtt acttgtcgtt tttcctgcaa ttcggggttt atttccacta aactcacatc
    14161 atcaggatac aatctggcgt ttttctccag tatctccgta atcggcatat ctcttcctac
    14221 cttcctgcct tgctacaatt attttactgt taaaagacat atcaatatct tttaagcaga
    14281 ttcttttttc tattatacag atttttacgc aattaacaac acttaaacct taattttgaa
    14341 cactgccttc tttattttgc aaaaatcatt aaccattccg cccggtttat gggcttcata
    14401 aggataaatc acacaaaaat cctgcgcctg cagggctata atactcatga cctgcccgtc
    14461 ataacacagc gcacagtctt tttcctccga ataaggttgc gatactttca gcatgttgcc
    14521 gtttgcaact gcaattctct cattaccttc aataacatag tgaatatcga tatactgcct
    14581 gtgcatttca aatcttatct cttcaacagg cttaacttct acttccatta cggtatagta
    14641 aaaatcatca ccgtcaacag caaccttgcc tgaaggactc tcggtattca aacccttcat
    14701 tatttccatc gcccgcatca gataatcatc catgcacgca ggaacacctg taccgtcaat
    14761 atttccgctt atcatttttc cgccctccct gatacacaat gccattatac tcttgatttg
    14821 tcggataaca acaactctat acaaagctca aagataattg ttaaataaca accgtcaaca
    14881 tttttacaat aaaaaactca catcaggcag gaataatata gtatactcgt aataaggata
    14941 tcaaaggaga ccaactatga aaagtcaaca gagaaataac gaatattgat gattcat
//
LOCUS       DAOSOM010000041        14660 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_4134_length_14660_cov_5.417939, whole genome shotgun sequence.
ACCESSION   DAOSOM010000041 DAOSOM010000000
VERSION     DAOSOM010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 14660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14660
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4134_length_14660_cov_5.417939"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /gene="rplS"
                     /locus_tag="PLF14_08315"
     CDS             <1..238
                     /gene="rplS"
                     /locus_tag="PLF14_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOT71323.1"
                     /translation="IQSYEGTLIARKGKGLTETINVRRISYGIGVERIIPLNSQKISS
                     IEVIRRGQVRRAKLYYLRDRVGKSAKVKEKLVTK"
     gene            322..1173
                     /gene="ylqF"
                     /locus_tag="PLF14_08320"
     CDS             322..1173
                     /gene="ylqF"
                     /locus_tag="PLF14_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HOT71324.1"
                     /translation="MNINWYPGHMTKAKKMITENLKLVDLVVEILDARIPYSSRNPDF
                     KELFSNKLQIIVLNKADLADPVTTEKWRKYYDKIDTPVILVDSKSGFGMRDFLNSVNK
                     VMRDKFEKDRAKGMKRRPVKVMVCGIPNSGKSTFINKLTGCSAARTGNRPGVTKDKQW
                     IKINPDIFLLDTPGVLWPKFDDDLVAINLALTHAIKDEILDSERLCIKLIDFLLKKYP
                     ENLMGRYNLIDINKDSHEILKDICENRKFLIPGGGPDIQKAASTVLDEFRKGQIGTIS
                     LEEPDDF"
     gene            1163..1900
                     /locus_tag="PLF14_08325"
     CDS             1163..1900
                     /locus_tag="PLF14_08325"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012739282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOT71325.1"
                     /translation="MTFKELKDRFFAASLEKCRIMLSEYSESNFFDKASELLDKEEQR
                     LLDMCKYETFLQKSGISLIAGTDEAGRGPLAGPIVAAAVILKNNDLLFGINDSKKLSA
                     EKRDRLFDAILDNCIAASIHTITNKQIDEINIGKADRLALKTSVEKLTVIPDHVLTDA
                     FVIENLNINQTPVTHGDALSVSIAAASIIAKVSRDRMMNEYDKIYPRYGFAKHKGYGT
                     KEHIDAIKKYGICPIHRKTFVKKYTDI"
     gene            1935..3203
                     /locus_tag="PLF14_08330"
     CDS             1935..3203
                     /locus_tag="PLF14_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HOT71326.1"
                     /translation="MDYKKQYTIEKAKYEAFRNDGIKLDISRGKPCKKQIDYVAALFK
                     KAENEYDLYNNRIGVDKIDLGNYGLLPGITETRTLLGSILDIPPENIIIGGNSSLNLM
                     YDMLVINLMFGNPQSDIPWSRAGKIKFLCPVPGYDRHFMMTDHFGIEMINIPMHPDGP
                     DMDLVESIVAQDSSVKGIWCTPLYSNPDGYIYSDETVTRFAKMHTAAKDFRIFWDNAY
                     FTHHLVPDKINKIANIIKLCEEYGNPDRVYQFSSTSKISFPGAGVSLIASSLNNIKHI
                     QEHFKYKTICYDKMNMLRHTLVFKTKEDIEHHMENMSAFNRPKFDMVLNILDRELSDY
                     KEYLYYTRPEGGYFISLYTMKNCAKRTYQLCKDAGLIITNVGDTYPYGKDPDDSNIRI
                     APTFPDLNELEKAVNLLVCCIKLAILEKLI"
     gene            3338..4078
                     /locus_tag="PLF14_08335"
     CDS             3338..4078
                     /locus_tag="PLF14_08335"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOT71327.1"
                     /translation="MINKYSSTPLYYQLKEVLIKAINNGKYAPGEAIPSENELAAQYS
                     LSRPTVRQALGELVKMGYLIKIKGKGTFVADFINKVNYDYTKGFIHCLLDTIDTSNRK
                     IIESTTIVGTLELNKKFSTDFKSNFHEDYSKITYITETDENISYSVSMLPLNYFPNAA
                     ELMFKNVNSIDLLVGKYPLDPTSAKSSISLVNADTVIAKYMKLPENAPLFKVETFLYA
                     RTGNIVEFNTTYYNAYSSIIDISRTRRV"
     gene            complement(4698..6035)
                     /gene="mgtE"
                     /locus_tag="PLF14_08340"
     CDS             complement(4698..6035)
                     /gene="mgtE"
                     /locus_tag="PLF14_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010299664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOT71328.1"
                     /translation="MIDTILELLVQKDYKKLKALLAEENPADIAEVISELDENDAIVV
                     FRLLSKDMGADVFAYMDKDTHIDILSNINEEEITYLMNNLFIDDTVDIISELPANYVT
                     RVLQNVSESRRKLINHFLQYPDDTAGSVMTIEYMDLKPDMTVKEAIERVKKYAVRKET
                     VNNCYVIDDKRIMLGEVTLKDLILADYDEQISDIMNDDVIKANTLTSTDEISQMFTKY
                     DILSLPVVDTEDRLVGIITVDDVMELIEEDTSEEMTRMTGAAPLENPYLKTGVFGLAK
                     SRIIWLLVLMIGATVSQLIIGKYEEFLATYVVLAGFLPMLMDTAGNTGAQTTTLIVRG
                     LGINEIKFSDMPRVLFKEFRVSLVIGVLLAVANFLKLWLLQRIDPIIAAAVSLTLICT
                     ISIAMVAGSTLPFLAKKIKLDPAVAVSPMITTIVDAFAVLIYFAIAKALLGLV"
     gene            complement(6311..8686)
                     /locus_tag="PLF14_08345"
     CDS             complement(6311..8686)
                     /locus_tag="PLF14_08345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HOT71329.1"
                     /translation="MEEKYLKKIEYDKIIKILADYADSAPGKSSVERIFPSTEYDTII
                     SDLNTLKSAMYFIKVKGKPDFEGLTDVSGIIKRLSLQGVLSTKELLIIRDNSCIARRV
                     SETSQTLNPNNADEYDIKKIIEHIVPLYDIESEIRRCILSEEDIADDASKELLRIRNS
                     ILKNQEAIKDKLLSFTGSDKYRKYMQDNIITMRQDRYVIPIKSEYRNVVKGVIHDTSQ
                     TGSTLFIEPMEIVNMNNKIRELINDEKKEIERILRKLSEQCAIYKDEILSNFNICCNL
                     DVMFAKAAYAMSYSGTIPGINKNREIRLLNAKHPLIPEDIVVPIDFKIGKNFRILVIT
                     GPNTGGKTVTLKTVGLLTAMAHAGLAIPADEGSEICVFNSIYADIGDEQSIEQNLSTF
                     SSHMTNIINILKNADDLSLVLLDELGAGTDPVEGSALASAIIETLRNRNITTVATTHY
                     TELKTYASVTENVENASCEFDVKTLKPTFRLLIGIPGKSNAIYISRRLGLDEEIIKMA
                     KSSIDNKSSDYENVILSLEKSRQRMEKDRIKATEYKKEIEEIKKQLEKERDGIDKERD
                     KILEKAKKDAKKLIEKALDKAQHLINEIDELKKSNITVNAKTEADFRTTVRKTKEILE
                     IKDADQENDKDITTDDIKSGDTVMLRNLKQRADVLSNPDKDGNIIVQAGIIKMTVNIK
                     DVYKIDEQKEINKNLKMNVIKTSGPAKMELDIRGNTIFEAESKLDKFIEQGYFAGLHE
                     LSVIHGKGTGALRKGVHDYLKSNPYISSFRLGVYGEGESGITVIFLKDKNK"
     gene            complement(8726..9241)
                     /locus_tag="PLF14_08350"
     CDS             complement(8726..9241)
                     /locus_tag="PLF14_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71330.1"
                     /translation="MREKTKKLARSAILLAIMMILLYISTIIPGYKLAFYLLISLIPG
                     IVVIEYGIKQCIIFIVASFILSLVMPGDKMTLLLFYSFFGIYAVIKYLIEKIKSKVII
                     LIVKLVYFIGIFFLNMYFATKIAGILPEIMLKYNMWLVFAAAIIVFVIYDIMYTSFSQ
                     FYLNKIKKHVD"
     gene            complement(9254..9817)
                     /locus_tag="PLF14_08355"
     CDS             complement(9254..9817)
                     /locus_tag="PLF14_08355"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOT71331.1"
                     /translation="MDPYKVLGVNRNDSDETIKKAYRELVKKYHPDKYTDTDLKDLAN
                     EKLQQVNMAYDTIVKNRKSKDYGYSYDESSYKGSFAEVRRHILNRNYAAADNILRNST
                     NRNAEWHYLRGVIFQKQGWYDQAYSYFEKAHLMDPSNPEYATAYSQTRSTANAYRSNV
                     YTRGYSSADEDFCRLCQCLLCTSLCCR"
     gene            complement(9820..10686)
                     /locus_tag="PLF14_08360"
     CDS             complement(9820..10686)
                     /locus_tag="PLF14_08360"
                     /inference="COORDINATES: protein motif:HMM:NF039826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HOT71332.1"
                     /translation="MIGYIKTYPPELKIKEYVKYNAYYCGICHSIKNRWGQIPRLTIN
                     YDCVFLAVFIDSIKKKENNYSDFRCIVHPVMNKKKVINSDTVDYIADINIYLSYLKLI
                     DNIKDEKNIFHLSSERFMRIYGKKLRKKYAELTDKIDEHIEDMAILERERCDNIDQVA
                     ECYAKIVEDVCLFALKDIDENLKNVVGRIAYNVGNWVYTADAYDDIEKDIKKGMYNPY
                     LARFGYEKGRDIAEFKKEIKERCSFLLYAPLKAATDLYEDIDEELKDPIVKNIIYEGL
                     YDRTEKILNGEK"
     gene            complement(10688..11230)
                     /locus_tag="PLF14_08365"
     CDS             complement(10688..11230)
                     /locus_tag="PLF14_08365"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOT71333.1"
                     /translation="MKHIPNIITCIRILLIPVFCYYLWNDNAVVAGIVFIVASLSDVL
                     DGYLSRKLNAVSNFGKLADPFADKVMQISAIILLVLLNRMSTWIMIVIFVKDFILIAG
                     GLLIKFIFKITVTSRWFGKISAFYLNAVLAVSILFGMSRNVINILMIIALVMQVASLI
                     GYATLSLIEIKKINKEKGAV"
     gene            complement(11238..12209)
                     /gene="pfkA"
                     /locus_tag="PLF14_08370"
     CDS             complement(11238..12209)
                     /gene="pfkA"
                     /locus_tag="PLF14_08370"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOT71334.1"
                     /translation="MMKIKTIGVLTSGGDAPGMNAAIRAVVRTGIEDGITTYGISRGY
                     EGLINGEFEKLNARSVSGILQRGGTVLKTARSDRFKEKEYRDRAMKMCSVFGIEALVV
                     IGGDGSFRGALDFLREGMKCIVIPATIDNDISCSEYCIGYDTALNTALSAIDKLRDTA
                     YSHERVTVIEVMGRHAGYIAINDGIAGGADAIVIPEMEYNFQHDIIKPIMEGRNRGKR
                     DHCIVIAEGVGDTVNIAKEIENTTGIRTVCTILGYIQRGGSPMARDRVVASKMGFKAV
                     EVLNEGRFNRAICQRDNKIVDIDIEEALKMEKASFDYDMNLARVLAM"
     gene            complement(12241..>14660)
                     /gene="dnaE"
                     /locus_tag="PLF14_08375"
     CDS             complement(12241..>14660)
                     /gene="dnaE"
                     /locus_tag="PLF14_08375"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOT71335.1"
                     /translation="GSAAGSIVAYAMGITNVDPIRYNLLFERFLNPARITLPDIDVDF
                     CYERRSEVIEYVKNKYGQDKVCQIITFGTMAARGAIKDVGRALNLPYALCDKISKMIP
                     NELKITIDRALEVNPELLAEYQSDPSVKELIDMARKLEGISRHSGIHAAGVVITDNPV
                     KEYIPTQLSDDNSLVTQFPMDNLEELGLLKVDFLGLRTLTVIKDCIDLVRKNHGIDID
                     FDDMDYDDKNVFEYISSGNTSGIFQLESAGMTDFMKRFKPKSIEDITLGISIYRPGPM
                     KFIDMCLCNKNTPEGTVYEHELMKPILSDTYGCMIYQEQIMQICRDMAGYTMGESDLV
                     RRLMSKKKMSALLKEKDRFVRGAEKNNIPIEVANKLFDEMTDFGNYAFNKSHGVAYAY
                     ISYQTAYLKCYYPVEFMAATMNSFIATREKLAEYIEECRSINISVLPPNINKSDVKFT
                     VEGGNIRYPLAAINGVGIKACETIVSNRNQQGEYRNFVDFVKRNSRNDVNKKCIEGFI
                     KAGAFDGLGNNRCELFSCYEEVVDSINEQQKKNLEGQISLFAMVEDPTEHEYEIRKRE
                     EFDLSKKLIMEKEAIGLYMTGNPLDEYRSVLKDMNLFKCSYKNIDENDSDSVRLVDNE
                     RVKVAGIINSVQRKLTKNNEMMAFVVIEDLYDTMEVIIFPRTLEKYSSLLVPENKVII
                     NGKISLKQDTVKIIAEDIVELNVKKDDSITVNEDTADYRNNKENLRTDNGISFSDNQK
                     LYLNGDNINLSQLEAFVKYFTGNSALTIIKNVNGSKMVKTMYYNQSIDIINEMAELFG
                     KENMAFK"
BASE COUNT         4310 a         2928 c         2399 g         5023 t
ORIGIN      
        1 aatacagtct tacgagggca cgcttattgc aagaaaaggt aaaggactta ctgaaacaat
       61 caatgtaaga agaatatcat acggtatagg tgtggaaagg attattcctt tgaattcaca
      121 aaagatcagt tccatagagg ttataagaag gggacaggtc agaagagcta aactttacta
      181 tttgagagac agagtaggaa agtccgcaaa ggtaaaggaa aaacttgtaa caaagtaaga
      241 aatacgggac ttgttcccgt tttttttata tcatgtacaa tatagatata atgtataagc
      301 aacttacgaa gggtatatgt aatgaacatt aactggtatc ccggtcatat gactaaagca
      361 aagaaaatga taaccgagaa cttaaagctg gttgatctgg ttgtggaaat cttggacgca
      421 agaataccct actccagcag aaatcccgat tttaaagagt tgttcagtaa taaattacag
      481 attattgtac ttaataaggc ggaccttgca gatccggtta caacggaaaa atggcgtaaa
      541 tattacgata aaattgacac tcccgttatt ttagttgaca gtaagtcagg tttcggtatg
      601 agagattttt tgaattccgt taataaagtc atgagggata aatttgaaaa agacagagca
      661 aaaggtatga agagaagacc tgtcaaagta atggtatgcg gtatacccaa ttccggtaaa
      721 tccactttca taaacaaact gaccggttgt tctgcggcaa gaacggggaa caggccgggg
      781 gtgacaaagg acaaacaatg gataaaaata aatccggata tatttctttt ggacacaccc
      841 ggagtactgt ggcctaaatt tgacgatgat ttagtagcaa ttaatcttgc cttaactcat
      901 gcaataaaag acgaaattct tgattcggag cggttgtgta tcaaacttat cgactttctg
      961 ttaaagaaat atcctgaaaa tcttatggga agatacaatc ttatagatat aaataaagat
     1021 tctcatgaga tactcaaaga catttgtgaa aacagaaaat tcttaatacc gggaggaggt
     1081 ccggacattc aaaaagcagc ctcaacggtt cttgatgaat tccgtaaagg tcagataggc
     1141 acaatatctt tggaggaacc tgatgacttt taaagaatta aaagacaggt tttttgccgc
     1201 atctttagag aaatgcagaa taatgctttc ggaatattca gagagcaatt tttttgataa
     1261 agcttcagaa cttttggata aggaagaaca acgccttctt gatatgtgta aatatgaaac
     1321 ctttctgcag aaatccggca tctcacttat agcaggtacc gatgaagcgg gaaggggacc
     1381 gcttgcggga cctattgtcg cagcagccgt aatacttaag aataatgacc ttttattcgg
     1441 tattaacgat tccaagaaac tctctgctga aaaaagagac agattgtttg atgctattct
     1501 ggataactgt attgcagcaa gtattcatac aataaccaat aaacagatag acgagattaa
     1561 tataggaaaa gccgacaggc ttgccttaaa aacttcggtg gagaagctta ccgtgattcc
     1621 ggatcatgtt ctcaccgatg cttttgtaat agaaaactta aatatcaatc agacaccggt
     1681 tactcacggt gacgccttat ctgtttccat agctgcagct tctattattg ctaaagtatc
     1741 acgtgaccgt atgatgaatg agtatgacaa gatatatccc cggtacggtt ttgcaaaaca
     1801 taaaggatac ggtacaaagg aacatataga cgcaattaaa aaatacggaa tttgcccgat
     1861 acacagaaaa acatttgtga aaaaatatac agatatatga tataattagc cgaaaatatt
     1921 aaaaggattt aagcatggat tacaaaaagc agtatacgat agaaaaagcc aaatacgaag
     1981 cattcagaaa tgacggtatt aagttggata tatccagggg aaaaccctgt aaaaaacaga
     2041 tagattatgt ggctgcactt tttaagaaag ccgaaaacga gtacgacctt tataacaaca
     2101 gaatcggtgt cgataaaatt gatctgggta attacggact gttacccggc ataaccgaaa
     2161 caagaacact tttaggcagt atattggata ttccccccga gaacataata ataggcggta
     2221 attccagttt aaaccttatg tacgacatgc tggtaatcaa ccttatgttc ggtaatcctc
     2281 agagtgatat accctggagc agggcaggga agataaagtt tttatgcccc gtacccggtt
     2341 atgacagaca cttcatgatg accgatcact tcggaattga gatgataaat ataccgatgc
     2401 atcccgacgg accggatatg gatttggtgg agtcaatagt cgcccaagat tcctctgtga
     2461 aaggaatatg gtgcacaccg ctgtacagta atcccgacgg ttatatctat tccgacgaaa
     2521 cggtaacaag atttgcaaaa atgcatacgg cggcaaaaga cttcagaata ttctgggata
     2581 atgcatattt tactcatcat ctggttcctg acaagataaa taagatagca aatataataa
     2641 aattatgcga agaatacgga aatcccgaca gggtatatca gttttcttca accagtaaga
     2701 tttcctttcc gggcgccgga gtatctttaa ttgcttcctc attgaacaat ataaagcata
     2761 tacaggaaca ttttaagtat aaaacgatat gttacgataa gatgaatatg ctacgccata
     2821 cgcttgtatt taaaaccaaa gaagatattg agcatcatat ggaaaacatg tcggcattca
     2881 acagacctaa gttcgatatg gtgttgaata tattagaccg tgagttgagt gattataaag
     2941 agtatctgta ttacacaagg ccggaagggg gatactttat atctctttat acaatgaaaa
     3001 attgtgccaa aaggacctat cagttgtgca aagatgcggg ccttataata accaatgtag
     3061 gtgataccta tccgtacgga aaagatcctg atgacagcaa tatacgtata gctcctacat
     3121 ttcccgactt aaatgaactg gaaaaagccg ttaatttact ggtttgctgc attaaactgg
     3181 ctatattgga gaagttaata taaaacttga tttaatatct catcacggat attttaaatt
     3241 catttttcat tcgaataata aaatatcatt ataagctatt gatgttgttt taaatgtatg
     3301 atactatata gtcatcttgt aaggacaggt gaatatcatg ataaataaat acagttccac
     3361 accgttatac tatcagctta aggaagtact tataaaagca attaataatg gcaaatatgc
     3421 tcctggtgaa gcaattcctt cggagaatga gcttgccgca cagtacagcc tgtccagacc
     3481 taccgttcgt caggcactgg gtgaactggt aaaaatgggt tatcttataa aaataaaggg
     3541 aaaaggcacc tttgtagcgg attttatcaa taaagtcaat tatgattata caaaaggctt
     3601 tattcattgc ttattggaca caatagatac atcaaacaga aaaatcattg aatctacgac
     3661 aattgtaggt acattggaat tgaataaaaa attcagtacg gattttaaat cgaattttca
     3721 tgaggattat tcaaaaatca catatattac cgaaaccgat gaaaatattt cctattccgt
     3781 atcaatgctt cctttaaact attttcccaa tgcagcagag cttatgttca aaaatgtcaa
     3841 ctctattgat ttactggtag ggaagtatcc cttggatcct accagtgcta aatcatcaat
     3901 ttcactggtt aatgcagata ccgttatagc caaatacatg aaacttccgg aaaacgcacc
     3961 gttatttaag gtggaaacat tcctgtatgc acgaacaggt aacattgtag agttcaatac
     4021 tacatattac aatgcataca gttccataat tgatataagc agaaccagaa gagtttaaat
     4081 cttttctgta ttgttttgag gattgatatg aagatagata acagtaatat aatttttgca
     4141 atcgcaatca tatttatagt actggtagca atctggatat atgtgatagt tttttgctat
     4201 acctgaggga gggaaggtgg aaaaacttcc cttaattaat ttactgatga ttagacataa
     4261 tcataatttt gtctaatata gggaactgat tttaagcctt ttaaggcaaa aaagctgctt
     4321 ttcaatattt tccctctttt tttagcattt tatttctttt attacaaccc cgttcatcat
     4381 ttgcagtttt ttaaaatgtt ctatcggttt attctttctg atttattttt ttttcttaca
     4441 agtcttccga ttgcttttgt tccttataat catttcacag gttttaattg aaaatataat
     4501 tctctttatt tttcattatg ctgtttaatt cattacaatc aatctgatat attgttctat
     4561 tattttttta attccgtatg ttttaaatga agaacttacg ttcgtatttg tgtattctct
     4621 ttttatgttg aaaataagag gaatgattat tttatcattc ccctgccgaa aattataaat
     4681 tgaagtattc gtgttattta tactaatcct agtaatgctt tggcaatagc aaaatatatc
     4741 agtactgcaa aggcatccac aattgtagta atcatcggcg ataccgctac agccggatcc
     4801 agtttaatct tttttgccag gaacggcaat gtggaacctg caaccattgc tatggatatt
     4861 gtacatatta atgtaagaga aactgctgcg gctattattg ggtcaatacg ttgaagaagc
     4921 cataatttca agaagttggc tactgcaaga agcacaccga ttacaaggga gactctgaat
     4981 tccttgaaaa gaacccttgg catatcactg aatttaatct cattaattcc caaacctctt
     5041 acaatcaatg tggttgtctg agcccctgta tttccggccg tatccattaa cataggtaga
     5101 aatcccgcca acaccacata tgtggctaag aattcttcat atttaccgat tataagctgt
     5161 gatactgttg ctcctatcat taacaccaga agccatataa ttctgctttt tgccagtccg
     5221 aatacccctg ttttcaagta aggattttcc aacggcgcgg cacctgtcat ccttgtcatt
     5281 tcttcagaag tgtcttcttc aattaattcc attacgtcgt ctaccgtaat aatacctaca
     5341 agcctgtctt cggtatctac aacaggtaaa cttaatatgt catatttcgt aaacatttgg
     5401 gaaatctcat ccgttgaagt caatgtattg gctttaatta catcatcatt cattatgtca
     5461 cttatctgtt cgtcataatc cgctaaaatc agatctttta atgtaacttc accgagcatt
     5521 attcttttat catcaattac atagcagtta tttacggttt cttttcttac tgcatatttt
     5581 tttactcttt caatagcttc tttaaccgtc atatccggtt taaggtccat atactctatt
     5641 gtcataaccg aacctgccgt atcatcagga tactgtaaaa agtgatttat gagctttcta
     5701 cgggattctg aaacattctg cagcactctg gttacataat ttgcaggtaa ttcactgatt
     5761 atgtcgacag tatcatctat aaacagatta ttcataaggt aagttatttc ttcttcatta
     5821 atattcgata aaatatcaat gtgcgtatct ttatccatgt acgcaaaaac atcggctccc
     5881 atgtctttac ttaagagtct aaaaacaact attgcatcat tttcatccag ttcggaaata
     5941 acttcggcga tatccgcggg attttcctca gcgagtaatg ccttgagttt tttatagtct
     6001 ttttgaacaa gtaattctaa tattgtatca atcatgtcaa tcctttccgt taaagggctt
     6061 taaacatgac cgatcagccc tctaacttac cattattaaa tttcgttcat aactcgtact
     6121 gtccggtaag ggcgtttcca ataaaaaatc cttcctttgt tcaatattag ataaaagttg
     6181 tttatcctta attattctat atcattgatt atgcggtgtc aaaataaatt ttaattattc
     6241 aataataatt tacgataaaa ttattgaaat tttggattat aaaaaaatcg tctttaagaa
     6301 taattaaaaa ctacttattt ttatccttaa gaaatataac ggttattccc gattcgcctt
     6361 cgccgtatac tcccaacctg aaggaactga tataaggatt ggattttaag taatcatgaa
     6421 ccccttttct taatgctcct gtaccttttc cgtgtataac cgataattcg tgcaatccgg
     6481 caaaataacc ctgctcgata aacttatcca gcttggattc cgcttcaaaa atcgtattcc
     6541 cgcgtatatc cagttccatc tttgcaggtc cggaagtttt aattacattc atctttaagt
     6601 ttttatttat ttccttttgt tcatctattt tatatacatc ctttatatta acggtcattt
     6661 taatgattcc cgcctgaaca ataatatttc cgtctttatc gggattggat aacacatcgg
     6721 ctctctgttt aagatttcgc aacataacgg tatcaccgct cttgatatca tcagtagtga
     6781 tatctttatc attctcttga tctgcgtcct taatttccag tatttctttt gtttttctga
     6841 cagtagttct gaaatccgct tccgttttgg catttaccgt aatattgctc tttttaagtt
     6901 catctatctc atttataaga tgctgtgctt tatctaatgc tttttcaata agtttctttg
     6961 catctttctt cgctttttca agtattttat cccgttcttt atcaataccg tcccgttctt
     7021 tttccagctg ttttttaatc tcttctatct cttttttgta ttcagtcgct ttaattctat
     7081 ctttttccat tctttggcga cttttctcca gtgaaagaat aacattttca taatcgctgc
     7141 ttttgttgtc tatcgaactt ttcgccatct ttattatttc ttcgtctaat cccagtcttc
     7201 tggatatata tatggcatta ctttttcccg gtattcctat aagcaacctg aatgtcggtt
     7261 tcaaagtttt tacgtcaaac tcacagcttg cattctccac attttccgta accgaagcat
     7321 atgtcttcaa ttccgtgtaa tgtgttgttg caaccgttgt tatatttctg tttcttaatg
     7381 tttctattat agctgatgca agcgcggaac cttctacggg atcggttccc gcacccagtt
     7441 catccaacaa taccagactc aaatcatcgg catttttcag tatgttaata atattagtca
     7501 tatgtgatga aaatgtactt aagttctgct caatgctctg ttcatcgcct atatccgcat
     7561 aaatgctgtt aaaaacgcat atttcgctgc cttcatcggc cggaattgcc aatccggcat
     7621 gtgccattgc ggttaaaaga cctaccgtct taagagtgac ggttttacct cccgtattcg
     7681 gtcctgtaat tacaagtatt ctgaagtttt tacctatctt aaaatctatc ggaactacaa
     7741 tatcctcggg tataagcggg tgttttgcat ttaaaaggcg aatttccctg ttcttattta
     7801 taccgggaat ggtgccgctg tatgacattg cgtatgcggc ttttgcaaac atgacatcta
     7861 aattgcaaca gatattaaaa ttgcttaata tctcatcttt atatatggcg cattgctcgg
     7921 aaagttttct caatatcctc tcaatctctt tcttttcatc atttatcagt tcccttatct
     7981 tattgttcat attcactatt tccataggct ctataaacaa agtggaacct gtttgtgatg
     8041 tatcatgaat tacgcctttt actacatttc tgtattcgct tttgattgga atgacatatc
     8101 tgtcctgacg catagtgatt atattgtcct gcatatattt tctgtactta tcacttcccg
     8161 taaaagacag cagtttatcc tttatagctt cctgattctt taatatgctg tttcttattc
     8221 gcagcaattc ctttgatgcg tcgtcggcaa tatcctcttc cgaaagtatg catcttctta
     8281 tctccgattc aatatcgtac agtggaacaa tgtgctcaat tatcttttta atatcatatt
     8341 catctgcgtt attcgggttt aaagtttggc ttgtttcgct aactcttctt gctatacagg
     8401 agttatctct gattataagt aattctttgg tacttaatac cccttgaaga cttaatcttt
     8461 ttattatgcc gcttacatcg gtaagtcctt caaaatcagg ttttcctttt actttgatga
     8521 aatacatggc ggatttaaga gtgttaagat cggatataat agtgtcgtat tcggttgaag
     8581 gaaatatcct ttcaacactt gatttacccg gtgcagagtc cgcatagtcg gcaagtattt
     8641 ttattatttt gtcatattca attttcttta aatacttttc ttccatatgt tctctttttt
     8701 gttaactctt aaaatcttat ttcgttcaat ctacatgctt ttttatctta ttcaagtaaa
     8761 actgagaaaa acttgtatac attatatcgt agattacaaa tacgattatt gctgctgcaa
     8821 ataccagcca catattgtat ttcagcataa tctccggtaa tattcccgca atctttgtgg
     8881 cgaaatacat gtttaagaag aatatgccga tgaaatatac cagttttaca attagaataa
     8941 taaccttgga ttttatcttc tcaatcaggt atttaattac agcatatata ccgaaaaaag
     9001 agtaaaacaa cagtaatgtc attttatctc cgggcattac aagggaaaga ataaatgaag
     9061 cgacaatgaa aatgatacat tgttttatac cgtattcaat aacgactatt ccggggataa
     9121 ggcttatcaa taagtagaaa gccagtttat atcccggtat tattgtgctt atgtatagca
     9181 aaatcatcat tattgcaaga agtatggcgg atcttgcaag tttctttgtt ttttcacgca
     9241 ttttctgttt cctttatctg caacataagc tggtacaaag taaacactgg caaagtctgc
     9301 aaaagtcctc atccgcagaa ctgtaccctc ttgtgtacac attgcttctg tatgcatttg
     9361 cagtacttct tgtctgtgaa tatgccgttg cgtattccgg attggaagga tccatcaagt
     9421 gagccttttc aaagtatgag taagcttgat cataccatcc ctgtttctgg aatataacac
     9481 cccttaaata gtgccactcg gcattcctgt ttgtggaatt acgtaagatg ttatccgctg
     9541 cagcataatt acgattcagt atatgtcgtc ttacttccgc aaaactgcct ttataactgc
     9601 tttcatcata tgaataaccg taatctttgc tttttctgtt ctttacgatg gtatcatatg
     9661 ccatatttac ctgttgcagc ttttcgttag ccaggtcttt caaatcggtg tccgtatatt
     9721 tatcgggatg gtatttctta accaactccc tgtatgcttt tttaattgtt tcatcgctgt
     9781 cgttacgatt aacacctaag actttgtatg gatccaattc tatttctctc cgttcagtat
     9841 tttttccgtc ctgtcgtaaa gaccttcata tataatattc ttcacgatag gatcctttaa
     9901 ttcttcatca atatcttcat ataagtctgt tgccgctttt aaaggagcat acagtaaaaa
     9961 cgagcatctt tccttaattt ctttcttaaa ttccgctata tctctcccct tctcatatcc
    10021 gaaacgtgca agatagggat tatacattcc ttttttaatg tctttctcaa tatcatcata
    10081 tgcatctgcc gtatataccc agttgcctac attgtatgct attcttccga caacattctt
    10141 gagattctca tcaatgtctt tcagtgcaaa gagacagaca tcctcgacaa tctttgcata
    10201 gcattcagct acctgatcta tattatcgca tctttccctc tccagtattg ccatatcttc
    10261 tatatgctca tctattttat ccgtaagctc agcgtatttt tttcttaatt ttttcccgta
    10321 tattctcata aacctttcgg aagacaaatg aaagatattc ttctcatcct ttatgttatc
    10381 gataagtttc agataggaaa gatatatgtt tatgtccgct atatagtcga ccgtatcgct
    10441 gttaattacc ttttttttat tcataacggg atgaacaata caacgaaagt ccgaataatt
    10501 attctccttt tttttaatgg aatctatgaa aaccgctaaa aacacacaat cgtaatttat
    10561 agtaagcctc ggaatctggc cccatctgtt ttttatggaa tggcatattc cgcaataata
    10621 tgcattgtac tttacatatt cctttatctt caattccggc ggataagtct taatatatcc
    10681 tatcatctta taccgccccc ttctccttat taatcttttt tatctctatc agactcagtg
    10741 tagcatatcc tatcagtgaa gcaacctgca taaccaaagc tataatcatg agtatattaa
    10801 tgacatttct gctcatgccg aacagtatgc tcacggcaag aaccgcattc agataaaatg
    10861 cggaaatctt tccgaaccat ctggatgtaa ctgtgatttt aaatataaat ttaattaaga
    10921 gcccacctgc aatgagtata aaatctttaa cgaagataac tatcataatc catgtactca
    10981 ttctgtttaa aagcaccaac agtataatag cggaaatctg cattacttta tcggcaaacg
    11041 gatcggcaag tttaccgaaa ttactgaccg cattaagttt tctggaaaga taaccgtcca
    11101 atacatcgga taaagatgcg actataaaga cgatacccgc tactacagca ttgtcattcc
    11161 ataagtaata gcagaatacc ggaatcagta aaattcttat gcatgttatt atattaggta
    11221 tatgtttcat tatcctccta catagcaagt actctggcaa gattcatgtc ataatcaaac
    11281 gatgcttttt ccatttttaa ggcttcttca atatcgatat ccacaatctt gttatctctt
    11341 tgacatattg ctctgttgaa acgaccttcg ttaagcacct ctacggcctt aaatcccatc
    11401 ttactggcca ctaccctgtc tcttgccatc ggacttccgc ctctttgtat gtatcccaat
    11461 atagtgcaaa ccgttcttat tccggttgtg ttttcaattt ctttggcaat atttacggta
    11521 tctcctactc cttcggcgat tactatacag tgatctcttt tacctctgtt tcttccctcc
    11581 attatgggct ttattatatc atgctggaaa ttgtattcca tttcgggaat aactattgca
    11641 tctgcacctc ctgcaatacc gtcatttata gctatatatc cggcatgacg tcccataact
    11701 tcgataactg ttaccctctc gtgtgagtaa gcggtatccc tcaatttgtc aatagcggac
    11761 agtgcggtat taagagcggt atcgtacccg atacaatatt cggaacagga aatatcattg
    11821 tcaattgtag caggtatgac aatgcatttc attccttctc ttaagaaatc cagtgctcct
    11881 ctgaatgaac cgtcaccgcc tattactaca agagcttcta ttccgaaaac actgcacatt
    11941 ttcattgccc tgtcacggta ttctttttct ttaaatctgt cggatcgggc agttttaagt
    12001 acagtaccgc ctctctgaag tattccggat acgcttcttg cattgagttt ttcaaactct
    12061 ccgttaatca aaccctcgta tcctctgctt ataccgtatg tggtaattcc gtcttctatg
    12121 cccgttctta cgacagctct tattgccgca ttcattcccg gtgcatctcc tccgcttgtt
    12181 aaaactccga ttgtctttat tttcataatc cgtctcctga attaaaattt tatcacatat
    12241 ctacttaaat gccatatttt cttttccgaa aagttccgcc atttcattaa taatatcaat
    12301 actctgatta tagtacatgg ttttaaccat tttactgccg tttacattct taatgatagt
    12361 caaagcggag tttccggtaa agtactttac aaaagcttcc agctgcgaaa gatttatatt
    12421 gtctccgttt aaatagagtt tttgattatc ggagaaactg ataccgttat ccgttcgtag
    12481 gttttcctta ttgttccggt aatcggcagt atcttcattt acggtaatac tgtcatcttt
    12541 cttaacattc aactcaacaa tatcttccgc tattattttg acggtatcct gttttaggct
    12601 tatttttccg tttataatca ctttattttc cggtacaagc aaagaagaat atttttccaa
    12661 ggttctgggg aatattatta cctccatggt atcgtataag tcttctataa ccacgaaagc
    12721 catcatttca ttgttctttg tgagttttct ttgcacactg tttattatcc ctgccacttt
    12781 aactctttcg ttatcgacaa gcctgacact atcactgtcg ttttcatcta tgtttttata
    12841 actgcactta aaaagattca tatctttcag aacacttctg tattcatcta aaggattacc
    12901 cgtcatgtat aggcctatcg cttctttttc cataatgagt ttctttgaca ggtcgaattc
    12961 ctctctcttt cttatctcat attcatgttc cgtcggatct tccaccattg caaaaagcga
    13021 tatctgacct tcaagattct ttttctgctg ctcattgatt gaatctacaa cctcttcata
    13081 gcaactgaac aattcacatc tgttattacc caaaccgtca aatgcacctg cttttataaa
    13141 gccctcgata cactttttat tgacatcatt tctgctgttt ctcttaacaa aatccacgaa
    13201 attcctgtat tcgccctgtt ggtttctgtt acttactatg gtttcgcatg cttttatacc
    13261 gacaccgtta attgcggcta aaggataacg tatattccct ccttcaacgg tgaacttcac
    13321 atcactttta ttgatattgg ggggaagtac cgaaatattt atggaccggc attcctcaat
    13381 gtattccgca agtttttctc ttgttgcaat aaaactgttc atagttgcag ccatgaactc
    13441 aacgggataa tagcacttca gatatgcagt ctgataggaa atatatgcat atgccactcc
    13501 gtgggatttg ttaaatgcgt aatttccgaa atcggtcatt tcatcaaaca acttattggc
    13561 tacttcaata ggtatattat ttttttccgc tcccctcaca aatctgtctt tctctttcag
    13621 caatgccgac atcttctttt ttgacatcaa tcttctgacc agatccgatt cacccatcgt
    13681 gtatccggcc atatcgcggc atatctgcat tatctgctcc tgatatatca tgcatccgta
    13741 cgtatcggac aatataggtt tcatcagttc atgctcataa accgtacctt cgggagtatt
    13801 cttgttgcac agacacatat ctataaactt catgggtccc ggtctgtata tggaaatacc
    13861 caatgttatg tcttcgatgg acttgggttt gaaacgtttc ataaaatcgg tcattcccgc
    13921 actttccaac tggaatatac ctgatgtgtt gcctgaggat atatattcga aaacattttt
    13981 atcatcgtaa tccatgtcat caaagtcaat atctataccg tgattttttc tcaccaaatc
    14041 aatacaatct ttaattaccg tcagtgttct taagcccaag aagtccacct tgagtaatcc
    14101 caactcctcc agattatcca taggaaactg tgttacaaga gaattatcgt ccgataactg
    14161 agtaggaata tattctttga cgggattatc ggtgattacc acacctgctg catgtatacc
    14221 tgaatgtctg gatatgcctt ccagttttct tgccatatct atcagttcct ttaccgaagg
    14281 gtccgattgg tattccgcca acagttcggg attgacctct aaagctctgt caatggtaat
    14341 cttcagttca ttgggtatca tcttgcttat tttatcgcat agtgcatacg gcagattgag
    14401 cgctcttccg acatccttta tagctcctct tgccgccatg gtaccgaaag ttattatctg
    14461 acatacctta tcctgtccgt acttattttt aacatactct atgacttccg atcttctttc
    14521 ataacagaag tccacgtcaa tatcgggtaa agttattctt gcaggattga gaaatcgctc
    14581 aaatagcaga ttatatctga taggatccac attggttata cccatagcat atgccactat
    14641 acttcctgca gcactgcctc
//
LOCUS       DAOSOM010000042        14251 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_4290_length_14251_cov_6.207241, whole genome shotgun sequence.
ACCESSION   DAOSOM010000042 DAOSOM010000000
VERSION     DAOSOM010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 14251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14251
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4290_length_14251_cov_6.207241"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1436
                     /locus_tag="PLF14_08380"
     CDS             <1..1436
                     /locus_tag="PLF14_08380"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HOT71336.1"
                     /translation="LVIEYFDKSDVYSTENRLYYLKILANMLADSKKKILYEEKLYKF
                     AYFDEVTDLANRNMLNNYLEKLIQNSNETDKFAVFNIELDNLRTINDTFGHDIGEQVV
                     IKSVDILSELMKNCCFIARVTEEKFIVVAPACSDSKKIEERAKKIAESFKHPILLPGL
                     DIEALFVIVNIGIAVYPEDGSDVNTLMKNSDLANYEARSTDDRIVFCSDQLKGRVEEN
                     TLITHKLFNSLQNEEFSLEFQPQVSCISGKTVGIEALLRWTTSDKKRIPPDKFIPILE
                     QTGLIHNVGLWVLDQALLEHNRLIANGFPPLRVSVNLSVVQFRKDDFVDNVTKIIEKH
                     RVDPKYIELEITESMLSKNFADTVDKLVQFKKMGLNIAVDDFGKGYSSLHRLVLIPFD
                     RIKIDKSIIDNVTFEKKSANVAKIIITLAKTLKAVITAEGVETREQAHFVRDIACDEI
                     QGYYFSRPLPTEALEKFLKEDGTGVRG"
     gene            1441..4560
                     /locus_tag="PLF14_08385"
     CDS             1441..4560
                     /locus_tag="PLF14_08385"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOT71337.1"
                     /translation="MKYALILSILLYVFGCFYLVFGIYALFANVKNRVNRLFYYLTSC
                     LAIFAISYSISNSAATEEASTFWRAFAVFGWGVYYSILIHIILIITDNKKFLKNRLAM
                     ILIYVPSLVNIILFSPLSPLKEASYNMSQTRYGWINYMPSDILSIGIAFYFIFYSILA
                     TVLIAGWWKNNKSEVLMKRQLIYFLISVSVPLITGITSVVLPNVLAKHLLHKLLIVSM
                     IVPIIVLFFSLKKYGLFSERGKTLYVFQQGQLDSEGARIRLFETTALVFIIGGELSFL
                     IGYFGMGKNLESELFLSTLVFIMGLISRFIPLMSKRHIVQNGLYALVCIAGMLFYTLT
                     TADVGGLTTWTVYIIFLLYSVILDTKSHAIAFTVFVIMLQFLFWIISPEITVTVNGGE
                     YSRRIVIILISAFSVRYLSSEYIEKEKQYKRFAKEQQVLEKISTGFLSLNKENAEEKI
                     YEMFKMSYELLNFNHAYLVNFDSDYENATIINTFVNVVEGKKSFPFNPGMKIAVSDFP
                     LAKALIEQKQPVMCDDINTIRPDKYDELREYFKSRDINSYFALPIFVEKDLRGMLVIE
                     YYEKSDVHLRENRLYFLMIIANILGDTRQMNLYEEKLYDYAYFDETTKLANRNMLRKN
                     LVQLLQNVGQTDRIAVFNIEMDNLRMINDTFGHNVGVQIVLKCVEILKSLMKECCIIS
                     RVAEGKFVLVMPTTESNEQLKERARKIIDAFTHPILLPDLDLEALFVMVSVGISLYPE
                     DGGNADTLIKNADLAGYEAKSADERIIFCSEQLKDRVEENTLMTHKLFRSLENQEFSL
                     EFQPQISCSTNKTVGIEALLRWTTSDKKRIPPDKFIPILEQTGLIHDVGLWVLDQALM
                     EHNHLIANGFPPIRVSVNLSTVQFRRDDFVQNVADIIQKHGVDPKYVELEITESMLAK
                     NFADTIDKLHQLKALGVKIAVDDFGKGYSSLHRLLLVPLDRIKIDKSIIDDVTFEAKK
                     ADIVKIIINLAKNLMLHITAEGVETVDQADFMKDISCDEIQGYYYSRPLKTDALEEFL
                     KISGVEVLN"
     gene            4688..5008
                     /locus_tag="PLF14_08390"
     CDS             4688..5008
                     /locus_tag="PLF14_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009145932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HOT71338.1"
                     /translation="MDNYSRAGMLTDIYGSLLTEKQLEILSLYFYEDCSLFEISEIMG
                     ITRQAVHDVIKRSLNLLEDYENKLAIYSKLLENRDKEKEIKSMLSENRVDEALQILHE
                     IVEN"
     gene            5008..6348
                     /gene="ffh"
                     /locus_tag="PLF14_08395"
     CDS             5008..6348
                     /gene="ffh"
                     /locus_tag="PLF14_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018932700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOT71339.1"
                     /translation="MAIFEGLSEKLGAIIKSLRSSGRITENDIKNISREIRLALLSAD
                     VNYKIVKDFVSVISEKALHKDVLESLTPGQQVIKIVHEELIALLGGKNEKLIVSSDIP
                     NKYMLCGLQGSGKTTACGKLGNLLKKQNKRVMFVACDVYRPAAIDQLVILGKQLDIPV
                     YYENDNKDVVKIAKNAIDQARKSMTDILLFDTAGRLHIDEELMNEIQALQKAINPNET
                     LLVVDAMTGQDAVNIAEGFSSRLDLTGIILTKLDGDTRGGAALSIKTIVNKPIKFASV
                     GERMNDLEPFYPDRMASRILGMGDVLSLIEKAQQSFDEKKAAELEKKMATASFTLQDY
                     LEQLEQLKDMGNMQDLINMIPGGASLKNVKVDEKQIVRTKAVIQSMTVKERNNPKILD
                     AKRRKRIAAGSGTTVADVNRVLKGFEDMKLMMKKMTDMKGKKFKGMNMPKGFSF"
     gene            6396..6641
                     /gene="rpsP"
                     /locus_tag="PLF14_08400"
     CDS             6396..6641
                     /gene="rpsP"
                     /locus_tag="PLF14_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOT71340.1"
                     /translation="MVKIRLKRIGTKKNPFYRVVVADSRFPRDGRFIEEIGMYDPQSN
                     PSRFEIDMDKANAWIAKGAQCTETVRALIKKHTATEE"
     gene            6645..6875
                     /locus_tag="PLF14_08405"
     CDS             6645..6875
                     /locus_tag="PLF14_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOT71341.1"
                     /translation="MKELLETIVFALVDEKDQVVITEEEDKDGNTVLKISAAPNDVGR
                     IIGKNGNTITAIRKILRAYAVKQRKRVIVDVL"
     gene            6876..7391
                     /gene="rimM"
                     /locus_tag="PLF14_08410"
     CDS             6876..7391
                     /gene="rimM"
                     /locus_tag="PLF14_08410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOT71342.1"
                     /translation="MKKLLDIGIITGAHGVKGEIKVLPISADASNFHSLKKVLIKGTP
                     YIVTSVRFVKNQALLKLDTINDRTTADTCKGNYVSITREQAVPLEKDTYYMEDIIGCD
                     VFEKNEFTGVITDIIETGSNDVYDIDGSDGKYRRQILLPAIKSVVLNIDIVNKRIDVL
                     IPKGLLDDEYI"
     gene            7378..8124
                     /gene="trmD"
                     /locus_tag="PLF14_08415"
     CDS             7378..8124
                     /gene="trmD"
                     /locus_tag="PLF14_08415"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009431633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOT71343.1"
                     /translation="MNTFNIFTLFPDMFKVLDYGILGIAKDKGIIKINTVNIRDFSAN
                     KHKKVDDYPYGGGFGMLMTCQPVFDAVNSVPKAHTVYMSPKGKVLTQKRAMELSKINN
                     INILCGHYEGIDRRIVDSLVDEELSCGDYILSGGEIPAMILIDCVSRLIDGVLPQPES
                     YLEESHTDNLLESHQYTRPEVYNGMAVPSILLSGHHKNIEEFRLAQSIYITLLNRPDM
                     LRRRKITKTELKVFLIYYPQLKDDIIVYVE"
     gene            8142..9386
                     /gene="glyA"
                     /locus_tag="PLF14_08420"
     CDS             8142..9386
                     /gene="glyA"
                     /locus_tag="PLF14_08420"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013411836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOT71344.1"
                     /translation="MYKLDSIRKVDRELADAIQSEICRQRNNIELIASENFVSQAVMD
                     AMGTPLTNKYAEGYPGRRYYGGCEHVDKVESLAIKYACELFGADHANVQPHSGANANL
                     AVFFAALKPGDTVLGLDLSCGGHLSHGMPKNISGSYFNVVSYGVDKETYMLDYDEIRR
                     LAHEHKPKLIIAGASAYSRAIDFAKFKDIADEVGAYLMVDMAHIAGLVAVGLHQNPIP
                     YADFVTTTTHKTLRGPRGGLILCKEQHKKAIDSAIFPGTQGGPLMHIIAAKAVCFKEA
                     LSEEFKLYQQNTLSNSKALCSALMSNGIDIVTKGTDNHLVLADLRSINVTGKDAQNML
                     DTVHITCNKNGIPYDTQKPTVTSGIRLGTPAVTTRGMKDTEMKEIAHIIYKTLTDYDN
                     YRETAIKKVKELINRFPLYDEK"
     gene            9376..10659
                     /locus_tag="PLF14_08425"
     CDS             9376..10659
                     /locus_tag="PLF14_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOT71345.1"
                     /translation="MKNEPLAYRMSPRTLDEFVGQLHIVGEKKLLRRMIEADNLHSII
                     FFGPPGTGKTALARIIANMTKSAFVSLNAVTCGIADIKQIVADTKNLFLNPSGRTVLF
                     LDEIHRFNKAQQDSLLPHIENGTIVLIGATTENPYFEVNKSLLSRSTVFMLNPLTKED
                     IYTILKNALNDKERGYGNYDIEISEETLKRLASSSSGDARIALNALELAVLTTKCDKS
                     GKTVITNQVLKECMQNNAIRFDKDGEDHYDNISAFIKSMRGSSPDGALFYLGKALIAG
                     EDINFLARRIVICAAEDVGLANPNALVVAQSAAEAVRMIGMPEASLILAEAAVYVAVS
                     AKSNSCYAGISAVFDDLKNIETGETPMWLRNAPVAEMKQHGYSVGYKYAHDYEGNYVE
                     QQYLPDIIKDKIYYKPTDNGEEGKLTEWMKKRKQS"
     gene            10632..10898
                     /locus_tag="PLF14_08430"
     CDS             10632..10898
                     /locus_tag="PLF14_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71346.1"
                     /translation="MDEKKKAVLKRVLAWIYIILFIFVILNLMILDFYEEYFLVAYIV
                     LLIVYFAVFRNKPDEKTKDNNSIKDDGNNKGDDSTKNDSVADRE"
     gene            10911..12086
                     /gene="nifS"
                     /locus_tag="PLF14_08435"
     CDS             10911..12086
                     /gene="nifS"
                     /locus_tag="PLF14_08435"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004092505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HOT71347.1"
                     /translation="MKHIYLDNAATTQLDQDVLEKMKPFIRDYYGNPSSIYSFGRESL
                     KAIEQARETVASCFNCRSSEIYFTSCGTESDNWAIKGICSSHRAKGKHIITTNIEHPA
                     VLDTFRYMKKIGYDITLVPVEKNGIVNASDIEKAIRKDTILISVMFANNEIGTIQPIA
                     EIGAIAKKHNIVFHTDAVQAAGAIPIDVKALNIDLMSVSAHKFHGPKGVGALYIKRGI
                     VIDNLLHGGGQERTRRAATENVCGIVGLAEALRLSVENMEKNNAHIKAIRDAVKAGIE
                     AKVPYIIYNGDETNRLVNNINFCFKFIEGEALILLLDMKGICASSGSACASKSLDPSH
                     VLLALGLPHEIAHGSLRLTFGKDSSMNDVDYIVNTVAQTVDRLRQMSPLYESFIKEH"
     gene            12148..12576
                     /gene="nifU"
                     /locus_tag="PLF14_08440"
     CDS             12148..12576
                     /gene="nifU"
                     /locus_tag="PLF14_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HOT71348.1"
                     /translation="MYSAKVMDHFANPRNVGVLADANAVGQVGNAKCGDIMKIYMKIE
                     DNIIKDVSFMTFGCGAAIATSSMATELVKGKTVEEALTLTNKAVMDALDGLPPVKVHC
                     SLLAEEAIKAAIYDYKKSIGEQVTEDNFACNTCCHNKKCE"
     gene            12586..14034
                     /locus_tag="PLF14_08445"
     CDS             12586..14034
                     /locus_tag="PLF14_08445"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOT71349.1"
                     /translation="MVENKDIKDVLFYERKNVWKEINEEQVTSINHFSEGYKKFLDQC
                     KTERECVKYIHNLCLTKENSFISLKSAYKNRQQLHPGDKLYYIEHNKAIILITIGKEG
                     LSQGVKIIGSHIDAPRLDLKPLPIFEDTELGFLKTHYYGGIRKYQWVSVPLALHGIIY
                     KADGTEVEINIGESETDPIFVIPDLLPHLSAEQNKLPLKDAIPGESLNLLTGSVPNVD
                     TEVTDKVKATILQLLNNKYGITEKDFINAEIEAVPAMKARDVGIDKSFVGGYGQDDRV
                     CAYTSLMAFFDASNIINDKTCYCYFSDKEEIGSYGNTGAQCDTLTYVTELIAELLAPS
                     DKNDKQSINVKNVFRESVMLSADVAAAFDPNHKTVFEANNAAYINRGIALSKYNGAGG
                     KGGTSDANAEFVNAVTKLFDQSDDIVWQIAELGKIDAGGGGTISKFGAHLGINVLDAG
                     VPVLSMHSPYEITSKADIYMTYKAFKAFYENL"
BASE COUNT         4694 a         2415 c         3099 g         4043 t
ORIGIN      
        1 tgttggtaat agaatacttt gataagagtg atgtatattc caccgaaaac cgattatact
       61 atttaaaaat cctggcaaat atgttagccg attccaagaa gaaaattttg tatgaggaaa
      121 agttatataa atttgcatat tttgatgaag ttacagattt agcaaacaga aacatgctga
      181 ataactattt ggagaaactt attcaaaaca gtaatgaaac cgataaattt gcggttttta
      241 atattgagct tgataacctg agaaccatta atgatacatt cgggcatgat atcggagaac
      301 aggtggtaat taaatcggta gatattttga gtgagcttat gaagaattgt tgtttcatag
      361 caagagtaac agaggagaag tttatagttg ttgcacctgc ttgttccgat agtaaaaaga
      421 ttgaggaacg cgcaaaaaaa atagcagagt cttttaagca tcctattcta ctgcccggat
      481 tggatataga agcattattt gttattgtca atatcggaat agccgtttat ccggaagacg
      541 gaagtgatgt gaatactctt atgaagaact ccgatttggc gaattacgaa gccaggtcta
      601 ccgatgatag aattgttttc tgttccgatc aacttaaggg acgtgttgaa gagaatacat
      661 taataacaca caagcttttc aattcgttac agaatgagga gttttctctg gaatttcaac
      721 ctcaggtcag ttgtatttca ggtaaaacgg taggaattga agccttactc agatggacaa
      781 catcggataa aaagagaata cctcctgata aattcatacc aattctggag cagaccggtt
      841 taattcataa tgtgggacta tgggtattgg atcaggcact tttggaacat aacaggctta
      901 tagcaaacgg atttccccct ttgcgtgttt cggtaaactt atcggttgta caattccgca
      961 aagacgattt tgttgataat gttacaaaaa tcatagagaa acaccgggtt gatcctaaat
     1021 acattgagct ggagattacc gaaagtatgt tgtcgaagaa ttttgccgat acagtcgata
     1081 aactggttca atttaaaaaa atgggactta atattgccgt tgatgatttc ggcaagggat
     1141 attcatcgtt acacagattg gtattgatac cctttgacag aattaaaata gataaaagca
     1201 taatagataa cgtaactttt gagaaaaaga gtgctaatgt tgccaagata atcattacat
     1261 tagctaaaac ccttaaggcg gttattactg ctgaaggtgt agaaacacgg gagcaggcgc
     1321 attttgtaag agatatagcc tgcgatgaaa tacagggata ttatttttcc agaccgttgc
     1381 ctaccgaagc tttggaaaag tttcttaaag aagacggtac aggagtcaga ggataagatt
     1441 atgaaatatg cattaatttt atcaatattg ctttatgtgt tcggatgttt ctatctggtt
     1501 ttcgggatat atgctttgtt cgctaatgtt aaaaatcgtg taaacagatt gttttattat
     1561 ctgacatcat gcttggcaat ttttgccatt tcatactcca tatccaactc cgctgctaca
     1621 gaggaagcaa gcacattttg gagagccttt gctgttttcg ggtggggagt ttattacagt
     1681 atccttattc atattattct gattatcacg gataataaaa agtttttgaa aaaccgtttg
     1741 gcgatgattt taatttatgt gccgtcatta gttaatatta ttcttttttc accattaagt
     1801 cctcttaaag aagcaagcta taatatgagc caaacccgtt acggatggat aaactatatg
     1861 ccttcagaca tattatcaat cgggattgcc ttttatttca ttttttattc aattttggcc
     1921 accgttttaa ttgccggctg gtggaaaaat aataagtccg aagtccttat gaaacgtcaa
     1981 cttatttatt ttttaatatc cgtatccgtt ccccttatta cgggaataac atcagttgta
     2041 ttacccaatg ttcttgcaaa acatctactt cataaattat tgattgtttc tatgattgtg
     2101 cctattatag tattgttttt ttcattaaag aagtacggac tgttcagtga aagaggaaag
     2161 acactatacg tttttcaaca gggacagttg gattcggaag gcgcccgtat acgtttattc
     2221 gaaaccacgg cacttgtatt tataatcggc ggtgaattat ccttcttgat cggatatttc
     2281 ggaatgggaa agaatttgga gtccgaactt tttctctcta cattggtttt tataatggga
     2341 ttgatttcaa gatttatacc tctcatgtct aagagacata ttgttcagaa cggtctctat
     2401 gcattagtat gcatagcagg tatgctgttt tatacattga caactgcaga tgtcggcggg
     2461 ttgacaacct ggacggttta tataattttc cttctatatt cggttatact tgatacaaaa
     2521 tcccatgcta ttgcttttac cgtatttgtc ataatgctgc aatttctttt ttggattatt
     2581 tcccctgaga ttactgttac agtaaacggc ggtgaatata gcagaagaat tgttattata
     2641 ttaatatctg ctttttccgt cagatatctg agttccgaat atattgaaaa agaaaaacaa
     2701 tataaaagat ttgccaaaga gcaacaggtt ctggagaaaa tttccaccgg ctttctttcc
     2761 cttaacaaag agaatgcgga agagaaaata tatgaaatgt ttaaaatgtc ttatgaatta
     2821 ctgaatttta atcatgcata tctggtgaac tttgactcgg attatgaaaa tgcaaccatt
     2881 attaatacat ttgtgaatgt tgtcgaggga aagaagtcat ttcctttcaa cccgggtatg
     2941 aaaattgcgg tttccgattt tcctcttgct aaagctctca tcgaacaaaa acaaccggta
     3001 atgtgcgatg atatcaatac aatacgtccg gataagtatg atgaattgcg agaatatttt
     3061 aagtcgagag atatcaattc ttatttcgca ttaccgatat ttgtagagaa agatttgagg
     3121 ggaatgctcg taattgaata ttatgagaaa agtgatgtgc atttaagaga aaaccgctta
     3181 tactttttaa tgattatagc taacattttg ggtgatacga gacaaatgaa cctgtatgaa
     3241 gaaaagttgt acgattacgc atattttgac gaaaccacaa aattggccaa cagaaatatg
     3301 cttcggaaaa atctggtgca actgttgcaa aatgtcggac aaaccgacag aatcgcggtt
     3361 ttcaatattg agatggataa tctgagaatg attaatgata ccttcggcca taatgtaggg
     3421 gtacagatag ttttaaaatg cgtggaaatt ttgaaaagtc tgatgaagga atgttgcatt
     3481 atctccagag tggcggaggg gaaatttgtt ctcgttatgc ctactacgga aagtaacgag
     3541 caattaaaag aacgtgcccg aaaaataata gatgctttta ctcatcctat tttactgccc
     3601 gatttggatt tagaggcttt atttgttatg gtcagtgtag gtatatctct ctatcctgag
     3661 gatggcggta atgccgatac ccttataaaa aatgccgatc tggcagggta cgaagcaaag
     3721 tctgcagatg aaagaattat tttctgctcc gaacaactaa aggaccgtgt tgaggaaaat
     3781 accttaatga cacataaact tttcaggtca ttggaaaatc aggagttttc actggaattc
     3841 cagcctcaga tcagttgtag tacaaataaa acagtaggga ttgaagcatt attgagatgg
     3901 acaacatcgg ataaaaagag aataccgccg gacaagttta tacccattct ggaacagacc
     3961 ggtttaattc atgatgtggg actgtgggta ctggatcagg ctcttatgga acataatcac
     4021 cttatagcaa acggttttcc gcctatacgc gtttctgtaa acttgtctac tgttcagttc
     4081 cgaagagatg atttcgtaca gaatgttgcc gatattatac aaaaacacgg ggttgatccc
     4141 aaatatgtgg agttggaaat taccgaaagt atgttggcta agaactttgc cgatacaatt
     4201 gataaactgc atcagttaaa agcactcgga gtcaagattg cggtggatga tttcggtaaa
     4261 ggttattctt cgttacacag gttgttattg gttcctcttg acagaattaa gattgataaa
     4321 agtattatag atgatgtaac atttgaagca aaaaaagcgg acatagtgaa aataatcatt
     4381 aatttagcca aaaatctcat gttgcatatt accgcggaag gtgtggagac agtggatcag
     4441 gccgatttta tgaaggatat atcctgtgat gagatacagg gatattatta ttccagacct
     4501 ttgaagaccg atgcattgga agaattcctc aagattagcg gagtagaggt attaaattag
     4561 tcagaatttc taaagaataa cttattgaaa atatgaataa gttttaacac ttattatttt
     4621 tttcatattg acaacattca tatagtgctg tatcatgtgt caagtaatat tactttacag
     4681 gagttttatg gataactaca gcagggcagg gatgcttacg gatatatacg gttcattact
     4741 taccgagaaa caattggaaa ttctcagcct gtatttttat gaagattgct cattgtttga
     4801 gatatcggaa ataatgggta ttacccgtca ggccgtacat gacgttataa agaggtcttt
     4861 gaacctgctg gaagattacg aaaacaaact tgcaatctac tcaaaactgt tggagaacag
     4921 ggataaagaa aaggaaatta agtccatgct ttcggaaaac agagtagatg aggctttgca
     4981 aatactgcat gagatagtgg agaattaatg gctatatttg aaggcttaag cgagaagctg
     5041 ggagctataa taaaaagttt aagaagcagc ggacgtataa cggagaacga tattaaaaac
     5101 atatcccgtg agattcggct tgctttgttg tctgctgacg ttaattacaa aatagtcaaa
     5161 gattttgttt cggtcatatc ggaaaaagct ttacataaag atgtattgga aagccttact
     5221 cccggacagc aggtcattaa aatagtccat gaggaattga tagctttatt gggaggtaag
     5281 aacgagaaac ttatagtctc atcggatatt ccgaacaaat atatgctgtg cggactgcag
     5341 ggctcgggta aaaccacagc ctgcggaaaa ttagggaatt tgttgaaaaa gcagaataaa
     5401 agagtgatgt ttgtcgcatg tgatgtctat cgtcctgctg caatagacca gcttgttatt
     5461 ttaggtaaac agttggatat tcccgtatat tatgagaatg acaataagga tgtagtaaaa
     5521 atagccaaaa acgctattga tcaggcaagg aagtccatga ccgatatttt gctctttgat
     5581 acggcgggaa gacttcatat tgatgaagaa ctgatgaatg aaattcaggc tttacagaag
     5641 gcaataaacc caaatgaaac actgcttgtt gtggatgcca tgaccggtca ggatgccgtc
     5701 aatatagccg agggattctc ctccaggctg gatcttaccg gtataatact taccaaactg
     5761 gacggagata caagaggcgg tgcggctctc tccattaaaa cgatagtgaa taaacccatt
     5821 aaattcgctt cggtcggaga gagaatgaac gatctggaac cgttttatcc ggacaggatg
     5881 gcatcacgta tattgggtat gggtgacgtt ctttcgttga ttgaaaaagc tcaacagagt
     5941 ttcgacgaga aaaaagccgc tgaactggag aaaaagatgg ctaccgcctc gtttacactt
     6001 caggattatc tggaacagct tgagcaactt aaggatatgg gaaatatgca ggatctgatt
     6061 aatatgattc ccggcggagc gtcgcttaaa aatgtaaaag tggatgaaaa acaaattgta
     6121 agaactaaag ccgtcattca gtcaatgacc gtcaaagaga ggaataatcc taaaatttta
     6181 gatgccaaaa gaagaaagag aattgcagct ggcagcggta caacggtggc ggatgtcaac
     6241 agagtactga aaggctttga agatatgaaa cttatgatga aaaaaatgac cgatatgaaa
     6301 ggaaagaaat tcaaaggaat gaatatgccg aaaggatttt ccttttaaga gcttattatt
     6361 acttttttga ataaataatt aacggaggtt ggaaaatggt aaaaatcaga ttgaaaagaa
     6421 tcggtacaaa aaagaatcct ttttacagag tagtggtagc ggattccaga tttcccagag
     6481 acggcagatt tatcgaagag ataggtatgt atgatcctca gtcaaatccg tccaggtttg
     6541 aaatagacat ggataaagcc aatgcctgga tagcaaaagg tgctcagtgt accgaaacag
     6601 tcagagcttt gattaaaaag catactgcaa cggaggaata agaaatgaaa gaattactgg
     6661 aaactattgt ttttgctctt gttgacgaaa aagatcaggt agttataacc gaagaagagg
     6721 ataaagacgg taataccgtt cttaaaatat ccgctgctcc caatgatgtc ggaagaatta
     6781 tcggtaagaa cggtaatacc ataacggcca taaggaaaat tctcagggca tatgcagtca
     6841 aacagagaaa aagagtaata gtggatgtac tttaattgaa gaaattattg gatataggaa
     6901 taataacggg tgctcacggc gtaaaaggtg agataaaagt cttacctata tccgccgatg
     6961 catccaattt tcattccctt aaaaaagtat taatcaaagg cactccatat attgtaacct
     7021 ctgtcaggtt tgttaagaat caggcattat taaagcttga cactataaat gacaggacaa
     7081 ctgccgatac ctgcaaaggt aattatgtat ccataacaag agagcaggcc gttcctttgg
     7141 aaaaagatac ttactatatg gaagatatta tcggttgcga tgtttttgag aaaaatgagt
     7201 ttacaggtgt aattaccgac attattgaaa caggcagcaa cgatgtttac gatatagacg
     7261 gatctgacgg caaataccgg cgacagatac ttctgcctgc tataaaatcc gtcgttctga
     7321 acattgatat tgtaaataag aggatagatg ttttgattcc gaaaggtctt ctcgatgatg
     7381 aatacattta acatattcac gctgtttccc gatatgttca aagtactgga ttacggaata
     7441 ctgggaatag cgaaggataa aggaataatt aaaataaata ctgtcaatat acgtgacttc
     7501 tccgcaaata aacacaaaaa ggtggatgac tatccttatg ggggaggttt cggtatgctt
     7561 atgacctgtc agccggtttt tgatgccgtt aacagcgttc cgaaagctca taccgtatac
     7621 atgtcaccga aaggcaaagt actgacacag aaacgtgcaa tggagttatc taagattaat
     7681 aatataaata ttctctgcgg tcattatgaa ggaattgaca gacgaattgt cgattcattg
     7741 gttgacgaag aactatcctg cggcgactat attctttcgg gaggggagat acctgcaatg
     7801 attctcattg actgtgtaag caggttgatt gacggtgttc ttcctcagcc ggaaagttat
     7861 ctggaagagt ctcatactga caatcttctg gaaagtcatc aatacaccag accggaagtg
     7921 tataacggaa tggccgtccc atcgatattg ctttccggac atcataagaa tatagaagaa
     7981 ttccggcttg ctcagagcat ttatatcact ctgcttaaca gaccggacat gctcaggaga
     8041 cgaaagatta caaaaaccga acttaaagtg tttttgatat attatcctca attaaaagat
     8101 gatataattg tttacgttga ataaatgaaa tggagaactt catgtacaaa ctggattcta
     8161 ttcgtaaggt agatagagaa ttagcagacg ccattcaatc ggaaatttgc agacaaagaa
     8221 acaatattga acttatcgct tcggaaaatt tcgtgtccca ggccgttatg gatgcaatgg
     8281 gtacgccttt aacaaacaaa tatgccgaag gatatccggg cagaagatac tacggcggat
     8341 gcgagcatgt tgataaggtg gaatcattgg ctataaaata tgcatgcgaa cttttcggtg
     8401 cagatcatgc caatgttcag cctcattcgg gagccaatgc caatttagcg gtgttttttg
     8461 cagcgcttaa acccggtgat accgttttag gtttggattt atcctgcgga ggacatctgt
     8521 cccacggtat gcccaaaaat atatcgggaa gctattttaa tgtcgtttcc tatggtgtgg
     8581 acaaagagac ttatatgctg gattatgatg agataagaag attagcccat gaacataaac
     8641 ccaaactcat tattgccgga gcttcggcat attccagagc aatagatttt gccaaattta
     8701 aagatattgc cgatgaagta ggagcttatc ttatggtgga tatggctcac atagccggtc
     8761 tggtggcggt gggcttacat cagaatccta ttccttatgc cgattttgtc actacgacaa
     8821 ctcacaagac actgcgcggt cccagaggcg gccttatact ttgcaaggag caacataaaa
     8881 aggcaataga cagtgcgata ttccccggaa ctcagggcgg tccgctgatg catataattg
     8941 cggcaaaagc tgtctgtttc aaagaagcct tatccgaaga attcaaatta tatcagcaga
     9001 atacattatc caattccaaa gctttatgca gtgcattgat gtcaaacggc atagatatcg
     9061 tcacaaaagg aaccgataat catcttgtac tggcagatct gagaagtatt aacgttaccg
     9121 gtaaagatgc tcagaacatg ctggataccg tacatataac atgcaataag aacggaattc
     9181 cctatgatac acagaaacct acggtaacca gcggtattcg cttaggaacc cccgcggtaa
     9241 ctaccagggg aatgaaagat acggaaatga aagagattgc tcatattatt tacaaaacat
     9301 tgaccgatta tgataattac agagaaacag ccattaagaa agtcaaggaa ctgattaaca
     9361 gatttccttt atacgatgaa aaatgaaccg ttagcttaca gaatgagtcc gagaactttg
     9421 gatgaattcg tgggacaact tcatatagtc ggagaaaaga agcttttgag aagaatgata
     9481 gaggcggata atctccactc cattatattc ttcggtcctc cgggaacggg gaagaccgct
     9541 ttggccagga taatcgccaa catgaccaaa tccgcttttg tatccttaaa cgccgttacc
     9601 tgcggcatag cggatattaa acagatagta gccgacacga aaaatctgtt tttaaatccc
     9661 tccggtcgaa ccgtactgtt tctggacgag atacacagat tcaataaagc tcaacaggat
     9721 tcattattgc ctcacatcga aaacggaacc attgtgttaa taggtgcaac gacggagaat
     9781 ccgtattttg aagtaaataa gtctttatta tcccgttcca ctgtatttat gttgaatcct
     9841 cttacaaaag aagatatata taccattctg aagaacgcat taaatgataa agaaagagga
     9901 tacggaaatt acgatataga gatttccgaa gagacactga aaagacttgc ttcttccagt
     9961 tccggtgacg caagaattgc tttgaatgct ttggaattgg ctgtacttac aaccaaatgc
    10021 gacaagtccg gcaaaactgt tatcacaaac caagtactaa aagaatgtat gcagaataat
    10081 gccattcgtt tcgataaaga cggagaagac cattatgata atataagtgc gtttatcaaa
    10141 tccatgaggg gctcctctcc ggacggagca cttttttatc tcggaaaagc acttattgcc
    10201 ggtgaggata tcaatttttt agcaagaaga atagtaatct gtgcggcaga agatgtggga
    10261 ttggccaatc ccaatgcttt ggttgtggca caatcggccg ccgaagctgt aagaatgata
    10321 ggaatgcccg aggcatcgtt gatactggct gaagctgcgg tgtacgtagc cgtatcggca
    10381 aaatccaatt cctgctatgc gggtatttcc gccgtattcg atgatttaaa gaatatagag
    10441 acaggtgaga ctcccatgtg gttgcgaaac gcaccggttg ccgaaatgaa acagcacggt
    10501 tattccgtag ggtataaata cgctcacgat tatgaaggaa attatgttga acagcagtat
    10561 ctgcctgata taataaaaga taagatttac tataaaccga ccgacaacgg tgaagaaggg
    10621 aaacttaccg aatggatgaa aaaaagaaag cagtcttaaa aagagtttta gcatggatat
    10681 atataatact ttttattttt gtaatcttaa accttatgat attggatttt tatgaggagt
    10741 attttcttgt tgcatatata gtattattaa ttgtatattt tgcggttttc agaaataaac
    10801 ccgatgaaaa aacaaaagac aacaacagta taaaagatga cggtaataat aaaggcgatg
    10861 atagcactaa aaacgatagt gttgccgata gagaatagac aggagttttt atgaaacata
    10921 tatatttaga caacgccgcg accacacaac ttgatcagga cgttttggaa aagatgaagc
    10981 cttttatcag ggattattac ggtaatcctt cgtcgatata ttctttcgga agagagagtt
    11041 taaaagcaat tgaacaggca agagaaacag ttgcatcctg cttcaattgc agaagcagtg
    11101 agatatattt tacttcctgc ggtaccgaaa gcgataattg ggctataaag gggatatgtt
    11161 cttctcacag agctaaaggt aaacatatca taaccacaaa catagagcat cccgcagttt
    11221 tggatacttt ccggtatatg aaaaagatcg gttatgacat tacgttggtc cccgtggaga
    11281 aaaacggtat tgtcaatgct tcggatattg aaaaagcaat acgcaaagat accatattaa
    11341 tctcggtaat gtttgcaaac aatgaaatag gaacaataca gcctatagcc gagataggag
    11401 caatagcaaa aaagcataat atagtgtttc atacagatgc cgttcaggct gcaggggcga
    11461 tacctattga tgtaaaagca ttgaatattg accttatgtc cgtttccgct cataagtttc
    11521 acggacctaa gggtgtcgga gccctgtata tcaaaagagg tattgttatt gataatcttt
    11581 tgcacggcgg aggacaggag agaaccagaa gagcggctac cgagaatgtc tgcgggatag
    11641 taggcctggc ggaagcactg agactgtcgg tggaaaatat ggagaaaaat aatgcgcata
    11701 tcaaggcaat aagagatgcg gtaaaagcag gtattgaggc taaggttccg tatattatct
    11761 acaacggtga tgaaacaaac agacttgtca ataacataaa tttctgtttt aagttcattg
    11821 aaggggaagc attgatactt cttctggata tgaaaggcat atgtgcttct tccggctcgg
    11881 catgcgcatc taaatcgctg gatccttcac atgtgctttt agcactggga ttaccgcacg
    11941 agattgcaca cggttctttg cgattgacat tcggcaaaga cagttccatg aacgatgtgg
    12001 actatatagt caatactgtt gctcaaacgg tggacagatt gagacaaatg tcgcctttgt
    12061 atgaatcttt cataaaagaa cactgatgat aactattata gctgccatat tatgctataa
    12121 tataaaaaat gaaaggagtg catgttaatg tacagcgcta aagtaatgga tcatttcgca
    12181 aatccccgca atgtgggagt gcttgccgat gccaatgcgg taggacaggt aggcaatgcc
    12241 aaatgcggag atattatgaa aatatacatg aagatagaag ataatattat taaagatgtc
    12301 agtttcatga ctttcggatg cggagcggca attgcaacaa gcagtatggc taccgagctg
    12361 gttaaaggca aaacagtaga agaagccttg acattaacca ataaggcagt tatggacgca
    12421 ctggacgggt taccgccggt taaggtgcat tgttcactgc ttgcggaaga ggcgataaag
    12481 gcggctatat atgactacaa aaagagtatc ggggaacagg tgacggaaga taactttgcc
    12541 tgtaatacct gttgccataa taaaaagtgc gaataggaga ataaaatggt agagaacaaa
    12601 gatataaaag atgtactttt ttatgaaaga aagaatgtct ggaaggaaat aaacgaagaa
    12661 caggttactt ccataaacca cttttccgaa ggatataaaa agtttttaga ccagtgtaaa
    12721 accgaaagag aatgtgtgaa atacatacat aatctttgtt taacaaaaga aaacagcttt
    12781 atatccctga aaagtgccta caaaaacaga cagcaactgc accccggaga taagctttat
    12841 tacatcgaac acaataaggc aattatcttg attacaatcg gtaaggaggg actttctcag
    12901 ggagttaaaa taataggttc acacatagat gctcccagac tggatttaaa acctctcccg
    12961 atatttgaag acaccgagct gggttttctt aaaactcact attacggtgg tataagaaaa
    13021 tatcagtggg tatcggttcc gctggctctt cacggaataa tttataaggc tgacggcacc
    13081 gaagtggaga taaacatagg tgagagtgaa accgacccta tcttcgtcat tcccgattta
    13141 cttccgcatc tttcggccga acagaataaa ttgcctttaa aggatgccat tccgggagaa
    13201 agtctcaatc ttcttacagg ttcggttccc aatgtcgaca cggaggtcac tgacaaggtc
    13261 aaagcgacca ttttgcagtt gcttaataac aagtacggta taaccgaaaa agacttcata
    13321 aacgccgaaa tagaggcggt tcccgccatg aaggcaagag atgtgggtat agataagtct
    13381 tttgtaggcg gatacggaca ggatgacaga gtatgtgcat atacatcatt gatggcattc
    13441 ttcgatgcaa gcaacattat taacgataag acctgctact gctacttctc ggataaagaa
    13501 gagataggtt cttacggaaa taccggagca cagtgtgata cattaactta tgtaaccgaa
    13561 ctgattgcag aattactggc tccttcggat aagaatgaca agcaaagtat taatgttaaa
    13621 aatgttttca gagaatcggt tatgctttca gccgatgtgg ctgccgcttt cgatcccaac
    13681 cataaaaccg tatttgaagc caataacgcg gcatatatca acaggggaat cgcactttcc
    13741 aaatataacg gtgcaggcgg taaaggcggc acaagcgatg ctaatgcgga gtttgtcaat
    13801 gccgtaacca aattattcga tcaaagtgac gatatagtct ggcagattgc ggaactgggt
    13861 aagattgatg cgggcggggg agggaccatc tccaaattcg gtgctcactt aggtatcaat
    13921 gtgttagatg ccggagtacc tgtattatcc atgcattcac cttatgagat taccagcaaa
    13981 gccgatatct atatgacata taaagcgttt aaagcatttt acgagaatct gtgataaagc
    14041 attgaaagaa agaaagtaaa agacctttcg gggtcttttt ttctttatat tctgtaattt
    14101 tttatttttt ttcttatgta tttctgaacc tgtcatcatt catctgcaat tattcaagtt
    14161 tggtataaaa agatttctgt ataattgtcg aagtagcagg aatatctatc tgataatatt
    14221 tggtaccgga tatttcttcc accgttccgg g
//
LOCUS       DAOSOM010000043        14177 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_4316_length_14177_cov_5.211797, whole genome shotgun sequence.
ACCESSION   DAOSOM010000043 DAOSOM010000000
VERSION     DAOSOM010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 14177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14177
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4316_length_14177_cov_5.211797"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(263..2635)
                     /gene="pheT"
                     /locus_tag="PLF14_08450"
     CDS             complement(263..2635)
                     /gene="pheT"
                     /locus_tag="PLF14_08450"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOT71350.1"
                     /translation="MKAPISWLRDFVDIDEDIKRFCNLMTVTGTKVEGYNQAGAEISG
                     VVTGKILTLRAHPNSDHLLIADVDVGDKVLQIVTGAPNVKIGQIIPIATDNSTLPKGV
                     TIKKGKLRGELSEGMMCSIQELNLTKHDYPDACDDGIWVLKEDTPLGMDIKKYAGLDD
                     CVVEFEITPNRQDCFSIKGIAREASAALDKPFKDKPIMLQEKGKGKADDYIKVGITAT
                     DLCSRYCAGIITDVIIEPSPDWMRSRLRNAGVRPVNNIVDITNYVLLECGQPMHAFDL
                     EFIEGNTINVRRAADNEKIITLDEKERILDRSVVVISDAKKAVALGGIMGAMNSEITD
                     NTKTVLFESAMFDSANIRISAKKLGMRSESSALFEKGLDANNALHAINRACELVELLN
                     AGKVVKGVVDVHKHLPVPVRIKLDAKRINQLLGTDIPVADMKSVLVKLGMRIDKDDII
                     VPTFRPDITGCADIAEEVARFYGYNNIVPSLTLGKSVTQGYRTHNQNVRKVIHDTLIA
                     CGLNETMTYSFISPKNLDKLGISEDSPMRDYLTIKNPLGEDFSVMRTTMISSMLKVLA
                     YNTNRSVKQGRFFEVSKTYHPAEILADEREVITVGLYGDCDFYDLKGIAENISESLGI
                     KDVEYVADRENPVFHTGRCANILLDNLVIGTLGQIDYSVSKEFDAPEDSYILTMDVSP
                     LYERATFTNTYMPIPRYPSSLRDLSIVVDRDVQSADIVKTVKKAVKGILENVTLFDIY
                     TGGQIEEGSKSLSYSFTFRAKDRTLEDQEVNKAMDKIMNTLSREFDAKVR"
     gene            complement(2645..3661)
                     /gene="pheS"
                     /locus_tag="PLF14_08455"
     CDS             complement(2645..3661)
                     /gene="pheS"
                     /locus_tag="PLF14_08455"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOT71351.1"
                     /translation="MLQLDVMLKNAEREILSATDMKKIEELKVQYLGKKGELTQVLRG
                     MSALAPEDRPAAGKAANDAREKLNQLFSDAEKIISDKAVKERLYEERLDVTVPGRKQD
                     IGHTHPLTQVIRKIENIFIGLGYQIAEGPEVESVHYNFDQLNAPDNHPSRDEQDTFYF
                     DAEKLLRTQTSPVQARVMENNAPPIRIICPGRVYRADEVDATHSPIFHQIEALVVDKD
                     ITMGNLVATLKLFAKEMFGEETEVRLRPHYFPFTEPSAEVDVSCWVCKGKGCPVCKYE
                     GFIEILGAGMVHPNVLRNCGIDPDIYSGFAFGLGVERTAMAVFGINDLRLFYENDARF
                     LSQF"
     gene            complement(4088..5437)
                     /gene="gdhA"
                     /locus_tag="PLF14_08460"
     CDS             complement(4088..5437)
                     /gene="gdhA"
                     /locus_tag="PLF14_08460"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018998675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOT71352.1"
                     /translation="MNITNPYIAKTYEQIISDNPNEPEFHQAVGEVFESIAPVIEAND
                     IYEKYAVLERIAEPERIISFKVTWVSDDGKINVNKGYRVQFNSAIGPYKGGLRFHPSV
                     NTSVLKFLAFEQVLKNSLTGLPLGGGKGGADFDPKGKSDMEIMRFCQSFMSELFRHIG
                     ADTDIPAGDIGVGSREVGYLFGQYKRLANQFNGVLTGKGLSYGGSLGRKEATGYGLCY
                     FTEEMLHAMRNDSFEYKDVAVSGSGNVAIHAIEKIYQLKGLPITASDSNGFIYDPNGI
                     NLDVLKQLKEVENRRIADYEKIVKGSIYYPSMKPWAIPCDIALPCATQNELNLEDAKN
                     LVNNNVMAVVEGANMPCTPDAIHYLRNNKVLFGPAKAANAGGVAVSGLEMSQNAARTN
                     WSFEEVDDKLRAIMKGIFNNINNTAKRYCVEGDFVAGANMAGFIKVADAMIAQGIIF"
     gene            complement(5606..6772)
                     /locus_tag="PLF14_08465"
     CDS             complement(5606..6772)
                     /locus_tag="PLF14_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOT71353.1"
                     /translation="MFVEILVDLYKESALSIITWQQGVLLVISLVLLYLGIVRQFEPL
                     LLVPIAFGMLATNLPGSNVYHPELWADMKNIDYNEVLKSGGLFDILYIGIKSGLFPPL
                     IFMGIGAMTDFGPLIANPKSFIIGAAAQLGIFLAFYLATLLGFTPAEGGAIGIIGGAD
                     GPTAIYTATILAPHLLGAIAVAAYSYMALVPVIQPPFMKLLTTKKERAIEMKQLRPVS
                     KKEKIIFPILVTIVVAFLVPSAAVLVASLMVGNLLKESGVTDRLSKTLQNEFINIVTI
                     CLGLAVGATATAENFLRAETLKILGLGVFAFIFGTIGGVLFAKLINLFSKNKINPLIG
                     SAGVSAVPMAARVSQKVAQEENPGIFLLMHAMGPNVAGVIGSAIAAGVLISLFG"
     gene            complement(6781..7122)
                     /locus_tag="PLF14_08470"
     CDS             complement(6781..7122)
                     /locus_tag="PLF14_08470"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HOT71354.1"
                     /translation="MSGSLLQTLIGFVVVNIGMILLTGIIMLFSKVMAKSGKKEIQAI
                     EEEKPVEHVLTVSDQSEEVIAAIMACVQAYIQQNGIKSACNIVIRPAVRTSVSAAWSN
                     AGRMENVSRRV"
     gene            complement(7178..7327)
                     /locus_tag="PLF14_08475"
     CDS             complement(7178..7327)
                     /locus_tag="PLF14_08475"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HOT71355.1"
                     /translation="MSEALLQTITGFSIVVLTLLLLTGIIAIFSKVMAKANAKNNQGV
                     EDENS"
     gene            complement(7342..8670)
                     /locus_tag="PLF14_08480"
     CDS             complement(7342..8670)
                     /locus_tag="PLF14_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HOT71356.1"
                     /translation="MLKITFMGAGSSVFVKNVIGDCMRTEALRDSEFALYDIDGQRLN
                     DSKMLIDSLNKNINDNRAKVTTYLGVKNRKDALRGADFVINAIQVGLYEPCTVIDFEV
                     PKKYGIRQTIADTLGIGGIMRALRTIPVMQDFADDIEAVCPDALFLNYTNPMSMLAGY
                     MQRYTDVRLVGLCHSVQVCSGGLLDKLGMQDKIEGRVDLIAGINHMAWLLEIKDKFGN
                     DLYPEIREKAAIKNATEKHNDMVRFEYIKHLGYYCTESSEHNAEYNPWFIKPAYPELI
                     ERFNIPLDEYPRRCIKQINKWAVQREELLHNTKTTHTRSNEYASYIMDAVVTNKPFKI
                     GGNVLNKGLITNLPEDACVEVPCMVDGNGINPCHVGKLPVQLAAMNMTNINVHLMTIE
                     AAVTRKREDIYRAAMLEPRCAAILSIDDIIKMVDELIETHQAAGFMKEYK"
     gene            complement(8778..10517)
                     /locus_tag="PLF14_08485"
     CDS             complement(8778..10517)
                     /locus_tag="PLF14_08485"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HOT71357.1"
                     /translation="MLEIYFNSRDTTYKSLFGAVQSGSTVKFRLDVRCDAPVEAYIVI
                     DKSKHRMHLERLIKDLSVFKINIQVSSKPGLMFYHFEISTPYHTVYYGNRPDTYQGQG
                     VKYDKNPLDYQITVYDNYNTPDWIKGRIMYQIFPDRFYKEKIHKSPYWDRIFHSDWYE
                     EPYIEEDFPGGKMECSDFFGGNLQGIIKKLDYIKSLGVGVIYLNPIYLAYSNHRYDAA
                     DYMSVDDTLGTKGDLKKLCEEAKKKDIFIILDMVFSHTGSDSIYFDKHKRFNKDGAFN
                     NPDSPYRSWYTFKQDGTYESWWGIETLPNTVETDPGFMSHVFDSIRQWLSLGVKGFRL
                     DVADELPDKFIRELRKVIKSCDKDAILIGEVWEDASNKISYGSRREYLYGYELDSVMN
                     YPYKNAIIDYTLNKINAESFVRRIEIIRENYPPQSYECLMNMLGTHDVSRILSIMGGK
                     GSLEKLPRKQQKGVFLSKEEYDIAFKRLSFCLQLLMCLPGVPCVYYADEAGSQGLKDP
                     FNRKTYPWGREDKKILDLYKEIMNNRYNDDKLKYGSFAIREDHGLVIITRKYQNESCE
                     YTFNNRHLSCSHS"
     gene            complement(10517..12946)
                     /locus_tag="PLF14_08490"
     CDS             complement(10517..12946)
                     /locus_tag="PLF14_08490"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOT71358.1"
                     /translation="MKNGNNKNTVIKQINEVLNRTFGKTIENATQNQLYKAFAITVKN
                     EIMDKWAQSAYTTDDKRVYYLSIEFLMGRALSNNILNIGKRKDYQEACKQLGLDFDSI
                     ASREPEAGLGNGGLGRLAACFMDSLATLDLPAYGCGIRYEYGLFRQKIIDGQQVEYPD
                     TWLEDGNVWEVERPEERVEVHFGGHIKESWEGDKLRFVHSGYSTVHAVPYDMPIVGYM
                     NDRVNSLRLWRAVSPKRIDMASFNRGEYLKAVEEKQMAEVLSHILYPEDKHYEGKSLR
                     LKQHYFFVSATMQMIVARFMKLNLPLSALPDKAVVHINDTHPALAIPELMRILMDEHG
                     LGWDEAWDITGRMFAYTNHTIMAEALEKWPEQLFKQILPRIYAIVKEINERFCGMVYR
                     DFPEKRNDIHKMAIIGYGQIAMAPLCIAGSYSVNGVSALHSRIIKEETFKDFADVFCG
                     KFTNVTNGVTHRRWLLLSNPGLTDLITKNIGKDWISDMTKIKALEAKADDPKFLDKFD
                     NIKRLNKRKLAEYIKQTTGIVVSENSIFDVQVKRLHEYKRQLLNILRIMYDYVQIINE
                     PEKYKDAIPVTCIFGAKAAPGYKRAKVIIKLINDAADLINNDPRVMDKIKVVFLENYS
                     VSLAQIIFPASDISQQISTASKEASGTGNMKFMMNGALTLGTMDGANVEIYESVGPDN
                     IYIFGYDAETVQQIYKTGEYDPTVFVEQDYTLKTVLNMLIDGTLNPEKPYLFKEIYES
                     LLSCKTGMSDPFLILGDFQSYKAAHDKIVKDYADRRKWNKMAVINVANSGRFSSDNSI
                     ANYNKLIWKLK"
     gene            complement(13095..>14177)
                     /locus_tag="PLF14_08495"
     CDS             complement(13095..>14177)
                     /locus_tag="PLF14_08495"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA];
                     GO_function: GO:0009011 - starch synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HOT71359.1"
                     /translation="LKLLDVQFDILHLNDWPTAMISLLLKEQYKNKHNGLYDNIKTIF
                     TIHNLKYQGIFDASVLSDLFGLSMDYMTTDKLEFFGRVNFMKAGLIYSDKITTVSNTY
                     KDEIRNPFFGENLDGVINTRSADLYGIVNGLDINEYNPKTDKNIYVNYGLSDIDKKKE
                     NKKNLQRELNLDLNENAPILAMITRLVPQKGLDLVKRVFDDMMSFNIQFVILGTGEEQ
                     YESFFENAHARYCGKVAALLKFDEQMARKIYAGADMFLMPSLYEPCGISQLIAMRYGT
                     IPIVRAVGGLCDTVTQYDSQNDTGNGYLFRNYNAHEMAHTVYLACQAYKGRTKWQKLM
                     KRAMKTDCSWDKAATQYINLYEIAMR"
BASE COUNT         3833 a         3397 c         2484 g         4463 t
ORIGIN      
        1 ctattccttc tttcatggtt tctccctgaa taaacttaat aacagcatta aaacaactaa
       61 gtaaatcaat tatataatca gtataatagt cgataatata attgtaaaca gtttgttctt
      121 taagtaaaat atcgtacttg gagctaaaaa caactgttta aaaaccaaag cgacacttgc
      181 agaataagaa aataccttct tcctgtaact gcgtcaggca actttatcaa tcggtcagac
      241 agtgcctgtt gaattaatgc tgctatctca ctttagcatc aaactccctg ctcaaagtgt
      301 tcattatctt atccatggct ttattaacct cttggtcctc aagggtacgg tcttttgccc
      361 tgaatgtaaa ggaatacgag aggctcttgc tgccctcttc tatctgtccg cccgtataga
      421 tgtcaaacag ggttacattt tccagtattc cttttacagc ttttttaaca gtcttaacta
      481 tatccgccga ttgcacatct ctgtccacta ctatggacaa atcacgtaat gatgacggat
      541 atctgggaat aggcatgtat gtatttgtaa aggttgctct ttcatacaga ggagacacat
      601 ccatagttaa aatgtaggag tcttccggag catcaaattc tttggaaacc gagtaatcaa
      661 tctgtcccaa tgttcctatt accagattat ccaaaagaat attcgcacat cttccggtat
      721 ggaatacggg attctctcta tccgctacat attcaacatc cttgattccc aggctttccg
      781 atatattctc ggcaatacct ttcaagtcat aaaaatcaca gtctccgtaa agacctacgg
      841 ttatcacttc cctttcatcg gccagaatct cggccggatg ataagtcttg gaaacttcaa
      901 agaatcttcc ctgctttaca cttctgttgg tattatatgc cagaactttc agcatgctcg
      961 atatcattgt tgtacgcatt acggaaaagt cttcgcccaa cggattttta atggtcagat
     1021 aatctctcat cgggctgtct tcacttatac ccaacttgtc aaggttttta gggcttatga
     1081 acgaataagt cattgtttca ttcaatccgc acgcaataag agtatcatgt attacttttc
     1141 tcacattctg attatgtgtt ctgtatccct gagttaccga tttacccaat gtcagtgacg
     1201 gtactatatt gttgtatccg taaaatcttg caacttcttc ggcaatatcc gcacaaccgg
     1261 taatatccgg tctgaatgtc ggaactataa tatcgtcttt atctatccgc atacccaact
     1321 tcaccagaac ggatttcata tctgcaacag ggatatcggt acccagaagc tgattaattc
     1381 ttttagcatc cagctttatg cgtacgggta cgggaagatg cttatgtaca tccacaactc
     1441 ccttcacgac tttacctgca ttaagcagtt ccaccagctc acatgctctg tttatggcat
     1501 gaagagcatt gttggcatct aagcccttct cgaaaagtgc cgatgattcg gaacgcatac
     1561 ccagtttctt ggcgcttatc cttatattcg ccgaatcaaa catagccgac tcaaacagta
     1621 cggtctttgt gttatcggta atctcactgt tcattgcccc catgattccg cccagcgcaa
     1681 ccgccttctt tgcatcggat atgactacaa ccgatcggtc caatattctt tctttttcgt
     1741 ccagcgttat tattttctcg ttatctgccg cacggcgcac attaatggta ttgccttcaa
     1801 tgaattccaa atcgaatgca tgcatgggtt gaccgcattc cagcaataca tagttggtaa
     1861 tatccacaat attatttacc ggtcttacac ctgcatttct cagcctggaa cgcatccagt
     1921 cgggagaagg ctctataatc acatcggtta ttattccggc acagtatctc gaacataaat
     1981 cggtagcggt tatgcctact ttgatatagt cgtcagcttt tccttttccc ttttcctgaa
     2041 gcattatcgg tttatcctta aaaggcttat ccagagccgc agaagcttct cttgctattc
     2101 ctttgatact gaaacaatcc tgtctgttgg gtgtaatttc aaattccaca acgcaatcat
     2161 ccaagcccgc atacttcttt atatccattc ctaaaggagt atcttctttc aatacccata
     2221 ttccgtcatc acacgcatcg gggtaatcat gctttgtcag attcagttcc tgaatggaac
     2281 acatcattcc ttcggaaagt tcccctctta atttgccttt ttttatcgta acacctttgg
     2341 gcaaggtgga attatcggtg gcgataggta taatctgccc tatttttaca ttgggcgcac
     2401 cggtaactat ctgcaatacc ttatctccca catccacatc ggcaattaac agatggtcgg
     2461 agttgggatg agctctcaat gtcagaattt taccggtaac aacaccggat atttccgctc
     2521 ccgcctgatt gtaaccttcc acttttgtac cggtcacggt catcagatta caaaacctct
     2581 ttatatcctc atctatatca acaaaatctc ttaaccaact tataggtgct ttcatatcta
     2641 cctcctaaaa ctgagacaag aatcttgcat cgttttcgta aaacaatcga agatcgttta
     2701 ttccgaatac cgccattgcg gtacgttcca cacccaatcc gaatgcaaat ccgctgtata
     2761 tatcgggatc tattccgcaa tttctcaaaa cattgggatg aaccataccc gctcccagga
     2821 tttcaataaa tccttcatat ttacataccg ggcaaccttt ccctttgcat acccagcagc
     2881 tgacatccac ttcggcactg ggttcggtaa acgggaaata gtggggacga agccgtactt
     2941 ccgtctcttc tccgaacatt tcctttgcaa atagtttcaa tgtggcaacc agattaccca
     3001 ttgtgatatc cttgtctaca accagtgctt ctatctgatg gaatatcggt gaatgcgttg
     3061 catccacctc gtccgccctg tatacccttc cgggacatat tattctaatg ggaggagcgt
     3121 tattctccat tacccttgcc tgtacaggcg aagtctgtgt cctcagaagc ttttccgcat
     3181 caaagtaaaa ggtgtcctgc tcatctctgg aaggatggtt gtcgggtgca ttgagctgat
     3241 cgaaattata gtggaccgat tccacttccg gaccttccgc tatctgataa cccaacccta
     3301 taaatatatt ctcaatcttt cttataacct gtgtcaacgg atgggtatgc cctatatcct
     3361 gttttcttcc cggcacggtt acatccagac gttcttcata aagcctctct ttaaccgctt
     3421 tatcgcttat aatcttttcc gcatcgctaa acaattgatt cagtttctcg cgtgcatcgt
     3481 tggctgcttt tcccgccgca ggacggtctt cgggagcaag agcactcata cctctcaaaa
     3541 cttgagtcag ctcgcctttt ttacccagat actgaacctt gagttcctct atctttttca
     3601 tatccgttgc cgaaagtatt tccctttcgg catttttcaa catgacatcc agctgtaaca
     3661 tttagctttc ctccgtaata taaaatgagc cttcatcccg gtagggcgaa agctgattca
     3721 aaccatgaga ctacacatcc gacaatgcaa gggacctata tcggtgtcct gccgtctcgg
     3781 cttactgcaa gttcggccga acaactccaa aaggaacttc cccgcaccgc ccgctacggt
     3841 gctctcagcc acgacaccgt ctctctgtaa gaaaacaagt aagggtactt tctttcttca
     3901 tagtatttat cgtattttat catattttgc gtgaaataca atattatttt tgttttattc
     3961 gttttaaacc caaactccag cccgataaaa tccgttaact taaaagaaat attgatataa
     4021 cgtaaaagac gcccttgggc atctttaacg gttcattcat tatatgtata aaaacagaga
     4081 gtttttatca gaaaattatc ccctgagcaa tcatcgcatc ggccactttt ataaatccgg
     4141 ccatattggc tccggcaacg aaatcgcctt ccacgcaata gcgttttgcg gtattgttaa
     4201 tgttgttgaa tatgcctttc atgatagctc tgagcttatc gtcaacctcc tcaaatgacc
     4261 agtttgtcct tgcggcattc tggctcatct ccagaccgga caccgcaact ccccccgcat
     4321 tagcggcttt tgcaggaccg aacaggacct tattgttcct aagatagtgt attgcatcgg
     4381 gtgtgcaagg catgttcgct ccttccacaa cagccataac attattgttt accagattct
     4441 tcgcatcctc cagattcaat tcgttctgag tagcacaagg aagagctata tcacacggta
     4501 ttgcccacgg tttcattgaa ggataatata tcgacccttt aactattttt tcgtaatcgg
     4561 caatccttct gttttccact tctttaagtt gcttaagaac atccagattg attccgttgg
     4621 gatcgtatat gaatccgtta ctgtcgctgg cggttatagg taaacctttc aactgatata
     4681 tcttttcaat tgcatgtata gcgacatttc ccgaaccgga tacggcaaca tccttatact
     4741 caaaagaatc atttctcata gcatgcagca tttcttcggt aaaatagcac agtccgtaac
     4801 ccgtagcttc ctttctgccc agcgaccctc cgtatgataa cccctttccg gtcagcacac
     4861 cgttaaactg attggcaagc cgtttgtatt gtccgaataa atagccgact tcacgcgacc
     4921 ccacccctat atcaccggcg ggtatatcgg tatccgctcc tatatgccgg aaaagttccg
     4981 acatgaaaga ctgacaaaac ctcattattt ccatatcgga tttgcctttg gggtcaaaat
     5041 ccgcaccgcc ctttccgcct cctaacggta atcccgtcag actgtttttt aacacctgct
     5101 caaaggctaa gaatttcagc accgaagtat tgaccgacgg atgaaacctt aaccctccct
     5161 tatacggtcc gattgccgag ttgaactgta ccctgtaacc tttattcaca tttatcttac
     5221 cgtcatcgga aacccatgta accttgaaag atataattct ttccggttct gcaattcttt
     5281 ccagcacggc atatttttca tatatatcgt ttgcttctat taccggagca atggactcga
     5341 atacttcccc taccgcctga tggaattccg gctcattagg attatcggaa ataatctgtt
     5401 catacgtttt tgcaatataa ggatttgtaa tattcattat ttcaatgccc ctattcaaaa
     5461 tgcctgcatt tttgcaattt ccccaattac aaaaatcatt ttagccatat taacatatgt
     5521 aaaagcatta ttcaatatca atattaaaaa aaacagggta tttaacgtac cctgtcagaa
     5581 tattttacaa taatataatt tacacttatc cgaataacga aataagcaca cctgccgcta
     5641 ttgcggaacc tataacgccc gcaacattgg ggcccatggc atgcattaaa aggaatatgc
     5701 cggggttctc ttcctgcgca accttctgtg aaacccttgc agccataggt acagccgaaa
     5761 cacccgcgga accgataagc ggatttatct tattcttact gaagagatta ataagctttg
     5821 caaacagtac tccgccgatt gttccgaata tgaatgcaaa tactcccagt cccaatatct
     5881 tcaatgtctc ggctcttaag aaattctccg cagtggctgt agctccgacc gccaaaccta
     5941 aacatatggt aacaatatta ataaactcat tttgcagtgt tttggataac ctgtcggtaa
     6001 caccgctttc cttaagcaga ttacccacca taagcgaagc aaccagcacc gcggcactgg
     6061 gaactaaaaa tgccacaaca attgtaacca gtatggggaa tattattttc tcctttttgc
     6121 tcaccggacg aagctgcttc atctctatag cccgctcttt tttggtggta agcaacttca
     6181 tgaacggagg ctgtataacc ggaaccaaag ccatatatga ataagccgct accgctattg
     6241 cacctaaaag atgaggggca agtatggttg cggtatatat tgcggtaggt ccgtccgctc
     6301 ctcctattat tcctatcgct ccgccctccg caggtgtgaa acctaacaat gtggctaaat
     6361 agaatgcaag gaatattccc aactgtgccg ccgcacctat tataaagctt ttgggattgg
     6421 cgataagcgg accgaaatcg gtcattgcac ctattcccat gaatatgaga ggcgggaaaa
     6481 gaccggattt aattcctatg tacaggatat caaaaagtcc tccgcttttc agaacttcat
     6541 tgtaatcaat attcttcata tccgcccata attccggatg atatacattg gaaccgggca
     6601 aattggttgc cagcattccg aatgctatgg gtaccaatag aagaggctca aactgtctga
     6661 caatacccag atataacagt acaagagata ttaccaacaa cactccctgc tgccatgtta
     6721 ttatggaaag tgccgattct ttataaagat ccaccaaaat ttctacaaac atattacccc
     6781 ctataccctt ctgcttacat tttccatcct gccggcatta ctccatgcag cggataccga
     6841 tgtccttacc gcaggcctta taactatatt acatgcactt ttaataccgt tctgctgtat
     6901 atatgcctgt acacaggcca ttatagctgc aattacttct tcggattgat cggaaacggt
     6961 taaaacatgt tcaacaggct tttcttcttc aatagcctga atctccttct taccgctttt
     7021 agccataacc ttggaaaaaa gcataataat tccggtcagt aaaatcatcc ctatattcac
     7081 aaccacaaag cctattaatg tctgtaacaa tgaacccgac atagcctaac ctccaaaacg
     7141 ccattctcgt gaaatatata ataaatcaag gttttagtca agaattctca tcctcaacac
     7201 cttgattatt cttagcatta gctttagcca taaccttgga aaaaattgct attattccgg
     7261 tcagtaacag taatgttaag actactatac taaaacccgt tatcgtctgt aataatgctt
     7321 ccgacacaat tcttcctccg tttatttgta ctctttcata aaacctgccg cctgatgtgt
     7381 ctctataagc tcatccacca tctttataat atcatctata gacaggatag ctgcacatct
     7441 cggttccagc atggctgctc tgtatatgtc ttccctcttt cttgtaacgg cggcttcaat
     7501 ggtcataagg tgtacattga tattggtcat attcattgcc gcaagctgta cgggaagttt
     7561 tccgacatga caaggattta taccgttacc gtccaccata cacggaactt ccacacatgc
     7621 atcttcgggc aggttggtaa tcagcccttt attcaacaca ttaccgccta ttttgaacgg
     7681 cttattggta acaacggcat ccattatgta ggaagcatat tcattgcttc ttgtgtgtgt
     7741 tgttttagta ttatgcagaa gttcctctct ctgaaccgcc catttattaa tctgttttat
     7801 gcatcttctg ggatactcat ccaaaggtat attgaatctc tctataagtt ccggataagc
     7861 gggtttaatg aaccaggggt tatattccgc attatgttcg ctggattccg tacagtagta
     7921 tcccaaatgc ttaatatatt caaatctgac catatcgtta tgcttttccg tagcattctt
     7981 aatggccgct ttttctcgaa tttccggata caaatcgttg ccgaatttat ctttaatctc
     8041 cagcaaccat gccatatggt ttattccggc aattaaatcc acccttcctt caatcttgtc
     8101 ctgcataccc aatttgtcga gtaatccgcc cgaacaaacc tgtaccgaat gacaaaggcc
     8161 taccagacgt acatccgtat atctttgcat atagccggca agcatggaca tggggtttgt
     8221 atagttaaga aacagggcat cgggacagac agcctctata tcatcggcaa aatcctgcat
     8281 aaccggaatg gtacgtaagg ctctcattat tccgcctatt cccaatgtgt cggcaatagt
     8341 ctgccttata ccgtattttt taggaacttc aaaatctatt accgtacagg gttcatataa
     8401 gcctacctga atggcattta tcacaaaatc cgcaccccgc aaagcatctt ttctgttctt
     8461 tacacctaaa tatgttgtaa cctttgctct gttatcatta atgtttttat ttaacgaatc
     8521 aatcagcatc tttgaatcgt tcagtctctg accgtcaata tcatacaatg caaattccga
     8581 atctctgagc gcttcggtac gcatacaatc gcctattaca ttcttaacga ataccgagct
     8641 tcccgcaccc ataaaagtta ttttcaacat atgaacctcc cgaaaatcca ctaaagttaa
     8701 tgttatcata caaacacaac tcaatcaaca ttgatagggt aaaaaagcaa ctgaaaaagc
     8761 cggaaaaaag gtgcacttca ggagtgcgaa caggacaaat gacgattgtt aaatgtatat
     8821 tcacaacttt cattttgata tttgcgggta attatgacaa gaccgtggtc ctctctgatg
     8881 gcaaaagaac cgtatttcag tttgtcatca ttatatctgt tgttcattat ttctttgtaa
     8941 aggtcaagta tttttttatc ttctcttccc caaggataag ttttgcggtt aaacgggtct
     9001 ttcagtccct gacttcccgc ttcatccgca tagtatacgc aaggcacacc gggcagacac
     9061 atcagcaatt gcaggcagaa agacaatctt ttaaaagcaa tatcatattc ttctttgctt
     9121 agaaatacgc ctttctgttg ttttctggga agtttttcca agctgccttt tccgcccatt
     9181 atcgacagta ttctggaaac atcgtgagtt cccaacatat tcattaaaca ttcatatgac
     9241 tgaggaggat aattctccct tattatctca attcttctga caaagctttc cgcatttatt
     9301 ttattaagag tgtaatcgat aattgcgttt ttatacggat aattcattac gctgtccagt
     9361 tcatatccgt aaagatattc ccgacggctg ccgtaactta ttttattact tgcatcttcc
     9421 cacacttcac ctattaagat tgcatcttta tcacaactct taatcacttt tcttaattcc
     9481 ctgatgaact tatcgggcag ttcgtccgcc acatccaacc tgaaaccttt gacacctaaa
     9541 gataaccatt gtcttattga gtcaaacaca tggctcatga atcccggatc ggtttccacc
     9601 gtattgggca atgtctcaat accccaccac gattcgtatg tgccgtcttg tttgaaagtg
     9661 taccagcttc tgtaaggtga atcgggattg ttaaatgctc cgtctttgtt gaatcttttg
     9721 tgtttgtcaa agtatatgct gtcacttccg gtatgggaaa ataccatatc caggataatg
     9781 aatatatcct tttttttggc ttcttcacac agttttttta aatcaccttt ggtacctaag
     9841 gtatcatcaa cactcatata gtctgcagcg tcatatctgt ggttggaata agctaaatat
     9901 atggggttca ggtaaatgac acccactcct aaagacttaa tatagtccag cttctttatt
     9961 atcccctgca gattacctcc gaaaaagtcc gaacactcca ttttaccgcc ggggaaatcc
    10021 tcttcgatgt aaggctcttc ataccagtcg gaatgaaata tcctgtccca gtaaggactt
    10081 ttatggattt tttctttgta aaacctgtcg ggaaatatct ggtacatgat acgtcctttt
    10141 atccaatcgg gcgtattgta gttatcgtat accgttatct gataatccag aggattctta
    10201 tcgtacttaa caccctggcc ctgataagta tccggcctgt tgccgtagta tacggtatga
    10261 taaggggtgg atatttcaaa atgatagaac attaatcccg gttttgaaga aacttggata
    10321 ttgattttaa atacagataa atccttgata agcctttcca gatgcatccg atgcttggac
    10381 ttgtcaatga caatgtatgc ttcaacggga gcatcgcatc tgacatccag acgaaatttc
    10441 acggttgaac cactttgtac ggctccgaac agtgatttat atgtggtatc tctgctgtta
    10501 aaatatattt ccagcattat ttaagcttcc aaatcagttt attatagttg gctatactgt
    10561 tgtccgagga aaaacgtccg ctgtttgcca cattgattac cgccatcttg ttccatttgc
    10621 gtctgtcggc ataatcttta actattttat catgtgccgc tttatatgat tggaaatcac
    10681 ccagtatcag gaagggatcg ctcatgcctg ttttgcagga cagcaacgat tcatatatct
    10741 ccttaaacag atagggtttt tccggattga gtgtgccgtc aataagcata ttcaaaacgg
    10801 tcttgagagt atagtcctgc tccacaaata ccgtaggatc atactctcct gttttatata
    10861 tctgctgtac cgtttcggca tcataaccga atatgtaaat attgtccggt ccgacactct
    10921 catatatttc cacattggct ccgtccatgg tacctaaagt taatgctccg ttcatcataa
    10981 acttcatatt gccggtgccg ctggcttctt tggaagcggt ggaaatctgc tgtgaaatat
    11041 cgcttgcggg gaaaataatc tgcgcaagag ataccgaata attctccaag aatacaacct
    11101 ttatcttatc cataacacgc gggtcattgt ttataaggtc cgcagcatca tttataagtt
    11161 tgattataac tttagccctt ttgtaaccgg gtgccgcttt agctccgaaa atacatgtca
    11221 cgggaattgc atccttatat ttctccggct cgtttataat ctgcacatag tcgtacatta
    11281 ttcttaagat attcagcagc tgtcttttgt actcatgaag tctctttacc tgaacatcaa
    11341 agattgagtt ctcggaaacg actattccgg tggtctgttt tatatactcg gccaatttac
    11401 gcttattaag tctcttaata ttgtcaaact tatccaaaaa cttgggatcg tcggctttgg
    11461 cttctaaagc tttaatcttt gtcatatcgc ttatccagtc tttaccgatg tttttggtta
    11521 taaggtcggt caatccggga ttggacagga gcagccatct gcgatgagta actccgtttg
    11581 ttacattggt aaacttcccg caaaatacat cggcaaaatc tttaaaggtc tcttccttta
    11641 taatcctgga atgtaacgcc gagactccgt ttacagaata ggagcctgca atgcacaaag
    11701 gcgccatggc aatctgtccg tagcctataa tagccatctt gtgtatgtca ttccttttct
    11761 cgggaaagtc tctgtaaacc ataccgcaga aacgttcatt tatttctttt actatggcat
    11821 aaatcctggg caatatctgc ttgaacagtt gctccggcca tttttccaac gcttccgcca
    11881 taatggtgtg gttggtatat gcgaacatac ggccggttat atcccacgcc tcatcccaac
    11941 ccaaaccgtg ttcatccata agtattctca tcagttcggg aatggccaga gcgggatggg
    12001 tatcatttat atgtactacc gctttgtcgg ggagtgcgct taacggaaga tttaacttca
    12061 tgaaacgtgc aactatcatc tgcatggtag cggaaacaaa gaagtaatgc tgtttcaaac
    12121 gcagcgattt gccctcgtaa tgcttgtctt cgggatacaa tatgtgagaa agcacttccg
    12181 ccatttgctt ttcttcaaca gctttgagat attcacctct gttaaaactt gccatatcga
    12241 ttcgcttggg agaaaccgct ctccataatc tcagggaatt gactctgtca ttcatataac
    12301 cgactatagg catatcatac ggtaccgcat gtactgtaga atatccgcta tggacaaaac
    12361 gaagtttatc gccctcccag gattctttta tatgaccgcc gaaatgcact tccactcgct
    12421 cttccggtcg ctccacttcc cagacatttc cgtcttccag ccaggtgtcg ggatattcca
    12481 cctgttgtcc gtcaattatc ttctgccgga aaagaccgta ttcatatcgt attccgcaac
    12541 cgtaagccgg aaggtccagt gttgccaatg aatccataaa gcatgcagca aggcgcccca
    12601 gtcctccgtt tcccaaaccc gcttccggtt ctcttgatgc aatactatca aagtccagcc
    12661 ccagctgttt gcatgcttcc tgataatctt tccgctttcc tatatttaag atattattgg
    12721 ataaggctct tcccattaaa aactcaatcg aaagataata cacccgtttg tcatccgttg
    12781 tataagccga ttgtgcccat ttatccatta tttcattttt aaccgttatg gcgaatgctt
    12841 tataaagttg attctgtgtg gcattctcta ttgtttttcc gaaagtcctg tttaaaactt
    12901 catttatctg ttttattacc gtatttttat tgtttccgtt tttcaaaaaa gtactccttt
    12961 accgagatga tattatacac aaaacacact acttgtacaa caacataaaa gtttattatt
    13021 atcaaaatat tgtgtttttc tttccggctg ttacaccaga caaagtgtcg gtccgtaaaa
    13081 tcctgtatac catattaacg catagcaatt tcatataaat taatgtactg agtggcggct
    13141 ttatcccatg aacaatcggt cttcattgct cttttcatca gcttctgcca tttagtgcgt
    13201 cctttatatg cctgacatgc caaatacacg gtatgagcca tttcatgggc attatagttc
    13261 ctgaaaagat agccgttccc cgtgtcattt tgcgaatcat actgcgtcac cgtatcacac
    13321 aatcccccta ctgcacgcac aatgggtatt gtaccgtatc tcattgcgat aagctggctt
    13381 ataccgcacg gttcataaag agagggcatc agaaacatat ccgctcccgc ataaatcttt
    13441 ctcgccatct gttcatcgaa ttttaaaaga gcagctactt taccgcaata tctggcatga
    13501 gcattttcaa aaaagctttc atattgctct tctccggtac cgagaattac aaactgaatg
    13561 ttgaaagaca tcatatcatc aaacactctt ttgaccagat ccagcccttt ctgcggaaca
    13621 agtctggtaa tcatagccaa tataggagca ttttcattta aatccaggtt gagttctctt
    13681 tgcagattct ttttgttctc tttctttttg tcaatatcac tgaggccgta gttgacataa
    13741 atgttcttgt cggttttggg gttatattca tttatgtcca gaccgtttac tatcccgtac
    13801 aaatctgcac ttctggtatt aatgacaccg tcaagatttt ctccgaagaa aggattccgt
    13861 atctcatctt tgtaagtgtt gcttaccgtt gttatcttat cggagtatat cagccccgct
    13921 ttcatgaaat tgactcttcc gaaaaactcc agcttgtcgg ttgtcatata gtccattgag
    13981 aggccgaata agtcggataa caccgatgca tcgaatatac cctgatattt cagattatgt
    14041 attgtaaata tagtctttat attgtcgtaa agcccgttat gcttgttttt gtactgctcc
    14101 tttaaaagta aagaaatcat ggcggtcggc cagtcattga gatgcagaat atcaaactgc
    14161 acatccagaa gtttcag
//
LOCUS       DAOSOM010000044        13770 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_4478_length_13770_cov_5.743420, whole genome shotgun sequence.
ACCESSION   DAOSOM010000044 DAOSOM010000000
VERSION     DAOSOM010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 13770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13770
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4478_length_13770_cov_5.743420"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            301..1356
                     /locus_tag="PLF14_08500"
     CDS             301..1356
                     /locus_tag="PLF14_08500"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOT71360.1"
                     /translation="MTYTIGIDLTFIKDHKKAVGNEISALSIVEGLHNNRGGQDFIIF
                     ANEDNAKDLKSMYKSFNVISTTKGFAFYNRFLPKFVKKYGVDVLYYPQYVRFMNFRLK
                     TKIVITTHGLIKGDDFLLDKLRPAMIRKAAKVVAASDFLKNELVKKRGVDPSKIVTIP
                     NPLANMTKSVDVVFKKKNILAVGSDKPQKNIMLAVKAFEKLVPIIEHDLILIGDISGK
                     GEIFRYIVDNNISNRIIVTGRVSRETLFGYYKNTDLFVNTSTYEGFGLAPLEAMAFKA
                     PVVSTPIPSISELGNVQFDAALNYEDDEREVANKMLAVLKSEKNTEYLENRAKRILDY
                     YSLDSISKKYLQVFENV"
     gene            1349..1756
                     /locus_tag="PLF14_08505"
     CDS             1349..1756
                     /locus_tag="PLF14_08505"
                     /inference="COORDINATES: protein motif:HMM:NF017416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOT71361.1"
                     /translation="MYRKQARQITIYSFVTPFGGMLNKDNRWVRYAEAIPWDEIEKIY
                     ASKFSNRGAPAKPLRKVLGAYILKEEYNFSEARIIKEINENPYLQYFIGLNEYTDKVP
                     VSASLIRSFSKRFTEQDKTEIERLLKEARKSLR"
     gene            1870..>2445
                     /locus_tag="PLF14_08510"
     CDS             1870..>2445
                     /locus_tag="PLF14_08510"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71362.1"
                     /translation="MRKSCVTIFLIIAIMLSMTACHKQSPTVTPKPSDSVEPTQTDSA
                     SPTPAEQPSESISPEPTETVSPEETARLEREQLELQRKEQDKEFYVPLKPLDKTGQKP
                     DGDDIRALYLVDSVLTKNHGSQEQPDWRFVRCEDANVQLYKTYVEALAANNTEKINEL
                     KYLQGSLTVLERVIGIILGTEMNAVVLNIKSG"
     assembly_gap    2446..2455
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2456..3729
                     /locus_tag="PLF14_08515"
     CDS             <2456..3729
                     /locus_tag="PLF14_08515"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HOT71363.1"
                     /translation="GDGVITMPMQAECIKQVQTEPARFGDIGYVLNLFKSYGIYTIGR
                     VTSFQDNTLAKYNEYQHAIKNPDGSIWLDDNRSAWVDPFNQWVQKYNIAAAKEAALAG
                     FDEINFDYVRFPEGRRWGAYYTQYMLLHEGKRSDIIIHDFLKDAYEALEPYNVNVSAD
                     IFGLVARQWDSEDCIKIGQNWYRITTVVDYICPMIYPSHYSTGWYGFEYPDKNPYGMV
                     LGAMKDSIEKNSAFENPAKIRLWIQDFTAKYLYPADAIYYYGYEQVYGEVRALRELGD
                     FTYMFWNNGVDYNPGKYIFPQDTDKYPLKDGDKDPIGRTPAMAAKEYLSVMSNIYDSN
                     QYMLYVLTPLDARDSDYDMWKDITLPGLKAYKISGYTVNSYIITDDAGKNADVSVTLR
                     YTKGESTEEIYETVIWKTVLENGIWKVYSGF"
     gene            3742..4497
                     /locus_tag="PLF14_08520"
     CDS             3742..4497
                     /locus_tag="PLF14_08520"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOT71364.1"
                     /translation="MKAVVLAAGKGRRIGSEKEGVPKAMRIVNGRPMIEYVVKELSFV
                     EEIILVVGYKKEQIYEHLGDKYSYAVQEEQLGTGHAVKCASEFFADYDGPVLITFGDM
                     PLLTTKTYKKVFKAYNDSIKKNNPAACVMLSVMLKDQSLPYGRVIRDRKRSFVEIVEE
                     PDCNEEQKKIKEVFFGVMLFNGKKLNWILNRMTNNNAQNEYYLTDLPALLKEEGERIK
                     ICSINDLEEVYGVNTVEDLKRVEDIMKARGAND"
     gene            4490..5896
                     /locus_tag="PLF14_08525"
     CDS             4490..5896
                     /locus_tag="PLF14_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HOT71365.1"
                     /translation="MIKFGTGGWRAVIGDGFTKANIQLLVQGLCDKIIASGVQHKPVI
                     IGYDRRFLSDIGAKWAAEVFCGNSIGVHFIYRNSPTPLIMFQVKQEKAYYGIAVTASH
                     NPAIYNGVKLFTQGGRDASLEVTDEIVRYIENVKKVHCMPFEKATEEGLLTVIDPFND
                     YIDEIMGFLDKSAIMKARLRVLLDPMYGVSKTSLQTILLTLRCDVDVINDRHDTLFGG
                     KLPSPSVATLRKLSTMVVEEGYDIGLGTDGDADRLGVIDDQGRFIHPNEILMLLYYYL
                     VKHKGWRGDVIRNIATTHVLDDMAADFGYKCHEVPVGFKHISAKMEETDAVIGGESSG
                     GLTVRGHIFGKDGIFAASLLIEMMAVTKKNLSLILKEIYDRYGYRVMSENSYAFKSED
                     KERLMKILYKDRLIPDFDVPVERLSYEDGLKVYFKNGGWLVARFSGTEPLLRVFAEMP
                     DQQQADDVCNRMKGFLGI"
     gene            5899..6813
                     /locus_tag="PLF14_08530"
     CDS             5899..6813
                     /locus_tag="PLF14_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT71366.1"
                     /translation="MILVTGGCGFIGSHITDALIEKKYKVLVMDNLSSGSMENLNSKA
                     QFVKGDITNTEDIEAVFKKYPIDAVCHHAAQISVAVSMENPVFDINTNIIGIINLLQA
                     CEKYKVKRFVFASSAAIYGNPVYLPIDENHPKNPLSFYGLSKLASEQCIRIFAQDTDM
                     TYAILRYANVYGPRQRAEGEGGVVAVFMNRMVANKECYIHGDGKQTRDFIYVKDIAAA
                     NVKALECHVSFTANVSTNVYTDIVTLFNVMKKETGYEKQAVYTQPRKGDIKDSYLKNT
                     GIHELLSWYPEYDLSKGLKETAEYFLHA"
     gene            6806..7192
                     /locus_tag="PLF14_08535"
     CDS             6806..7192
                     /locus_tag="PLF14_08535"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOT71367.1"
                     /translation="MLDFLKKNRIFFTYVLIGTIATLLDMAILYILSDLLSLNYILSN
                     CISVFCGISLSFFLNSRYNFKKTDRIAIKFIYFASVCIIGLLVGSSIFVFLYENLGIY
                     KFISKALSVIIAGVLQFLFNKHVTFR"
     gene            7212..7970
                     /locus_tag="PLF14_08540"
     CDS             7212..7970
                     /locus_tag="PLF14_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71368.1"
                     /translation="MKLKALKIILIILFLLSGTATFLFSPYYELKNITVEESAVNITD
                     TLGECGVVAGCNINLLLLSKGNILSGHLVKAEEQVMSDVNIKNLSIKRSGRNDITVSC
                     QSRKPVMCISHDDYVIYFDYDGTIIDTSQMKAEKAILIRGLDLDFFYPGRNILTCNYM
                     LEDISVLCSEIEKYDTANYTAIRTLINEIEMQENNRIIVKYDNRLDILMDMTDDVKYN
                     ANVLCAILSNMNQNSKGTVDFTVSDTPFFSPASE"
     gene            8098..9372
                     /gene="ftsZ"
                     /locus_tag="PLF14_08545"
     CDS             8098..9372
                     /gene="ftsZ"
                     /locus_tag="PLF14_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOT71369.1"
                     /translation="MLQFEMEMEQFAQIKVLGVGGAGNNAIDRMITSGLKGVEFISIN
                     TDKQALMNSKATTKIQIGEKLTKGLGAGSIPEIGERSAEESKDIIIDHIKGADMIFIT
                     AGMGGGTGTGAVPIIAKEARQLGILTVCIVTKPFTFEGRKRKENAEKGIDKLQSIVDT
                     LIIVPNDRLLQISDKKITILEAFLTADNVLFKGVQSISDLIAVPGLINLDFADVKTVM
                     TDAGIAHMGIGRGTGEDKCEQAAMEAIKSPLLETSIEGAKKLLVNITGGPDMGLFEVD
                     HAANKIYEAADESADIIWGATIDPNMTDQIMITIIATGFNDDVKEYKPSILFPKKEEK
                     TFTFKDVKEPEKEDIKPEEPEEEKTEEEADDLKETRLFDIHLPDEEDKQTDEESDTVS
                     KEVEDKKPEPVAQPEKKQRDDTITIPSFLRMK"
     gene            9543..11261
                     /locus_tag="PLF14_08550"
     CDS             9543..11261
                     /locus_tag="PLF14_08550"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT71370.1"
                     /translation="MAIGKEGVYKVREIRDIREAIFGAAEIYGDKDALLEKNKETDKY
                     EAHSFNDFVKDIKSLGTALCDMGLKDKRVIVMGENRYFWAVSYMAVVCGTGVVVPVDV
                     LLNQTDLKYIFESSKPEAVIFTDRKATEIRNLAEELSYIKYFINMDGDYTDNKRFFNV
                     TDLMAKGSKLVEEGDRTFIDAEIDTEETQIILFTSATTSVAKAVQLSHRNIASNMMAM
                     SSMIKVYPTDVFLSVLPLHHTYECTCGFLCAMSLGGAIGYAEGLRYIKKNLDELKPSI
                     ILSVPLLFEKTYKNIMKSAEKSGSLKKLETFIKIADTLRLGKKIRRKMFSSVIAGLGG
                     NMRLMISGAAGISPTVAKFYRSIGIDFLQGYGLTECSPIICLNSDVDFVDESAGKPLP
                     GVEVVILDKDADGIGEICAKGPNVMKGYMNMPEENAKAFEGGYFHTGDLGYMDENDFV
                     YITGRKKSVIVTKTGKNIFPEELEQKLDKSKYVEECLVFGVTDKDGDSIVTAAVYPAY
                     QIFEEEFNNTDKKFIHDKIMELVQEANSQVPTYKAIKKLVIRDNEFEKTTTRKIKRYG
                     ANYKVD"
     gene            11417..12712
                     /locus_tag="PLF14_08555"
     CDS             11417..12712
                     /locus_tag="PLF14_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT71371.1"
                     /translation="MKLKLNYKRTFLVGLAFMSICMFWQMYDFVIPLILTNKFQMRVD
                     LANAIMALDNVLGLFMLPLFGVLSDKVNTKIGRRMPFILVGTAVAAIFTVVLPIAANS
                     NNLVLFMIALCLVLIAMATYRSPAVALMPDVTAKPVRSQGNAVINLMGTLGGTIVLAL
                     KMIIKPGEGWDYSTFFIVVASMMVISVMILFVTVNENKYRKEMEDINYGDPIIEEPGP
                     EVTEEKPDKLPKDVRKSMILLLASIFLWVFGYNAVTTAFSQYVVNFFNLPESLAAGFL
                     MVATVTGFLTFLPVSYLTARIGRRKTILGALALGIILFTISFFIKEFTFFANFLFAGV
                     GVVAGSITVNTLPMVLEMSKGSTIGRYTGYYYTCTMSAQILTPVLMGRLFYLIGYQIL
                     FPYATLFLILSFITMLFVKHGDSKPQVKKNKLEQFDVED"
     gene            12715..13542
                     /locus_tag="PLF14_08560"
     CDS             12715..13542
                     /locus_tag="PLF14_08560"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOT71372.1"
                     /translation="MEWLIAAVMLIIIWMLMIWPSDYIKEEKNKLGKYLYAHRGLHND
                     EKGIPENSLAAFDAACVAGYGMELDIQFSKDMQIVVFHDDDLKRMCGVDKKVNELTYE
                     ELSQLSLKGTQERIPLFTEVLKLVDGRTPLVVELKTCRKYKELTEETYKILKSYKGDY
                     CVESFNPMIVRKYSKLDPTVLRGLLMTNYIKFGGVNKVLGFLLQEMLFNFIARPQFIA
                     CDHRALSIWGVNVVRFIFRPVMATWTIRNEEEFEKVKSFDIVIFEDFLPDPERCELN"
     gene            13587..>13770
                     /locus_tag="PLF14_08565"
     CDS             13587..>13770
                     /locus_tag="PLF14_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71373.1"
                     /translation="MNKKILIFLLCITVILLLVSCDKPRNNQENNNPIVAENIDEQGN
                     NRIVIKRIDGSSPLKMS"
BASE COUNT         4459 a         2381 c         3194 g         3726 t
ORIGIN      
        1 gcggagtata tccgtcggtt accaaggcat gcgagcttgt aattacaacc aaaaacaggc
       61 agaaatttaa caaaaccgat gcaattgagt ataataagta ctatgaaata tataaatcgc
      121 tgtatcccgt cttaaaagac gagtacagga atttacgaat gttgttgcag tgatttaact
      181 tttaataacc gtcggttaag gtgatttata ccgctacctt gtacttattt tgattgcata
      241 acgaatatat ttaatatata atctgattga ccctattttg tatgtttaac ggagactatt
      301 atgacctata ccataggtat cgacttgaca tttatcaagg atcacaaaaa ggctgtcggc
      361 aacgagatat ccgctttaag tattgttgag ggtttacaca acaatagagg cggacaggat
      421 ttcataatat ttgcaaacga agataatgcc aaagacttaa aaagcatgta caagtcattt
      481 aatgttatct ccacaacaaa aggtttcgcg ttttacaaca gatttttacc taagtttgta
      541 aaaaagtacg gtgtggatgt tttatattat ccgcagtatg tgcgttttat gaatttccgg
      601 cttaaaacca aaatagtcat tacaacacac ggtttaatca aaggtgatga ttttctgttg
      661 gataaactca gaccggcaat gataagaaaa gcggctaaag tggttgccgc ttcggatttt
      721 ctgaaaaacg agctggtaaa aaaaagaggt gtcgatccgt ctaagattgt caccataccc
      781 aatcctttgg ccaatatgac aaagagtgtg gatgtggttt ttaagaaaaa gaatatattg
      841 gcggtgggaa gcgataagcc ccagaagaat ataatgttgg cggtgaaggc atttgagaaa
      901 cttgtaccga ttatagaaca cgaccttata ctgataggcg acatatcggg caaaggagag
      961 atattccgat atatcgtgga taataatatt tccaacagga ttatagtaac cggaagagtc
     1021 agcagggaaa cattattcgg atactataaa aataccgatt tgtttgtcaa cacttcaaca
     1081 tatgaagggt tcggattggc tccgctggaa gccatggctt ttaaggctcc cgtagtcagt
     1141 acccccatac cttccatcag cgaattgggg aatgtgcagt ttgatgccgc cctgaactat
     1201 gaagatgatg aaagagaagt ggcaaacaaa atgctggcag tgctgaaaag cgagaaaaac
     1261 acggaatatc tggaaaacag agctaagaga atcctggatt attacagtct ggacagtata
     1321 agtaagaaat atttgcaggt atttgaaaat gtataggaaa caggcacgac aaataactat
     1381 ttattctttc gtaacaccgt tcggcggtat gctgaacaag gacaacagat gggtgcggta
     1441 tgccgaagca attccctggg atgagattga aaagatttat gcttccaagt tcagcaacag
     1501 aggtgccccg gcaaaacctt taaggaaggt tttaggagca tatatactga aagaggaata
     1561 taacttttcc gaagcaagga taatcaagga aataaacgag aatccttatc tgcagtattt
     1621 tatcggtctg aatgaatata cggataaggt tcccgtcagt gcttctttga tacgttcttt
     1681 cagcaaaaga tttaccgaac aggataaaac cgagatagaa cgccttttaa aggaagcaag
     1741 aaaaagtctc aggtagaaaa gctttttaag tgctttactt aacagcagta aaagtaatat
     1801 aataggcggt gataacacag ccttgtttgg tgtgtgtaca aaagaaaaaa cagtaaaacg
     1861 gagagatgaa tgagaaaatc ctgtgtaaca atctttttga taatagcaat tatgctttcc
     1921 atgaccgctt gtcataaaca aagcccgact gtaaccccta aaccttccga ttctgttgaa
     1981 cctacacaga cagattcggc aagtccgaca cctgctgaac aacctagcga gagtatttct
     2041 cccgagccta ccgagactgt aagtccggaa gaaacagcaa gactggagcg agaacagctg
     2101 gagttgcagc gaaaagagca ggataaggag ttttacgtgc cgttaaagcc gttggataaa
     2161 acagggcaaa agcccgacgg tgatgatatc cgggctttat acctggtgga ttcggttctt
     2221 acaaaaaatc acggttctca agaacagccc gactggaggt ttgttcgttg tgaagacgca
     2281 aatgtacagt tatataaaac atatgtggaa gcattggcgg ctaacaacac ggaaaagata
     2341 aatgaactga agtatcttca gggcagtctt accgttctgg aaagagttat aggaattatc
     2401 ttaggtaccg agatgaatgc cgtggtattg aatataaaga gcggcnnnnn nnnnngcggc
     2461 gacggagtta ttaccatgcc tatgcaggcg gaatgcataa agcaggttca gaccgaaccg
     2521 gccagattcg gcgacatcgg gtatgtactc aatttattca aaagctacgg tatttataca
     2581 ataggcaggg taacatcctt tcaggacaat acccttgcca aatacaatga ataccaacat
     2641 gccatcaaga atcccgacgg aagcatatgg ctggatgata accgttcggc atgggtggat
     2701 ccgtttaacc aatgggttca gaaatacaac attgcagcgg caaaagaggc cgcgcttgca
     2761 ggttttgacg agattaactt tgattatgtc cgatttcccg agggacgaag atggggcgcc
     2821 tactataccc aatacatgct gctgcatgaa ggtaagcggt cggacataat aatacatgat
     2881 tttctcaaag acgcatatga agcccttgag ccgtacaatg taaatgtcag tgccgacata
     2941 ttcggcctgg tagccagaca gtgggattcg gaggattgta taaaaatagg gcagaactgg
     3001 tacagaataa ccaccgttgt ggactatata tgtcccatga tttatcccag ccattattct
     3061 accggttggt acggttttga atatcccgac aagaatcctt acggaatggt tttaggtgca
     3121 atgaaagatt cgattgagaa aaattccgct tttgaaaacc ctgccaaaat ccgcttatgg
     3181 attcaggact ttaccgcaaa atatctttat cccgcagatg ccatctatta ttacggttat
     3241 gagcaggtat acggagaggt aagagcattg agagagctgg gagattttac ttatatgttc
     3301 tggaataacg gtgtggatta caatcccggc aaatatattt tcccgcagga taccgataaa
     3361 tatcccttga aagacggcga taaagacccg ataggcagaa ctccggcaat ggctgcaaaa
     3421 gagtatcttt cggtaatgag caatatatat gattccaatc aatatatgct ttatgtactc
     3481 actccgctgg atgcaaggga ttccgactat gatatgtgga aggatattac tttgcccggt
     3541 ttaaaggcgt ataaaatatc gggatacacg gttaattcct atatcattac cgatgatgcg
     3601 ggaaaaaatg ctgatgtttc ggttactttg cgatatacta aaggtgaaag taccgaggaa
     3661 atatatgaaa ccgttatctg gaaaacggta ttggagaacg gtatatggaa ggtgtattcg
     3721 gggttttagg agggttgttg tatgaaagcc gtggttttag ctgccggtaa aggcagaagg
     3781 ataggttcgg aaaaagaagg tgtgcccaaa gcaatgagaa ttgttaacgg cagacctatg
     3841 attgagtatg tggtaaaaga gctttccttt gtagaggaga ttattcttgt ggtaggatac
     3901 aaaaaagaac agatttatga gcatcttgga gataaatact cttatgcggt acaggaagag
     3961 cagctgggta ccggacatgc ggtaaaatgt gcaagtgagt tttttgccga ttacgacgga
     4021 ccggtcctca taaccttcgg agatatgcct cttctgacaa caaaaaccta caaaaaggta
     4081 ttcaaagcat acaatgacag tataaagaag aataatcccg cggcatgcgt catgctctcg
     4141 gtgatgctaa aagatcagtc attaccgtac ggaagggtaa tacgtgacag gaaaaggtcc
     4201 tttgtggaaa tagtggaaga gcccgactgc aacgaggagc agaagaagat aaaggaagta
     4261 tttttcggtg ttatgctgtt taacggaaag aaacttaact ggatactgaa ccgtatgacc
     4321 aacaataatg cacagaacga atattatctt accgaccttc ccgctttgtt aaaggaagag
     4381 ggagagagga ttaaaatatg cagtattaac gatctcgaag aagtatatgg cgtcaatact
     4441 gtagaggact taaaaagagt ggaagacatt atgaaagcaa gaggtgcaaa tgattaagtt
     4501 cggaaccggc ggatggcgag ccgttatagg agacgggttt accaaagcaa acatacagct
     4561 tctggtccag ggattatgtg acaaaattat tgcgtcggga gtacagcata aacctgtgat
     4621 aatagggtat gacagacggt ttttgtccga tataggagcc aaatgggctg cggaagtttt
     4681 ctgcggcaac tccatcggag tgcatttcat atacaggaat tctcccaccc cccttataat
     4741 gttccaggtc aaacaggaaa aagcatatta cggaatcgcc gtaaccgcaa gtcacaatcc
     4801 cgccatatac aacggggtaa aactcttcac ccaaggcggc agagatgcct ctttggaagt
     4861 aaccgacgag attgtgcggt atatagagaa tgtgaaaaaa gtacattgta tgccctttga
     4921 gaaggccacg gaagagggat tgttaacggt tatcgacccg tttaacgact atattgatga
     4981 aataatggga tttttggata aatccgccat tatgaaagcc cgactgagag tgcttttaga
     5041 tcccatgtac ggagtatcca aaacatcatt acagaccata ttgcttactt taagatgcga
     5101 tgtcgacgtt ataaacgaca gacatgacac gctgttcggc ggaaaactgc cttcacccag
     5161 tgttgcaact ttgagaaaac tttccaccat ggttgtggaa gaaggttacg atatagggtt
     5221 aggtaccgac ggtgatgccg acagattggg agtaatcgac gatcagggca gatttataca
     5281 tccgaacgag atattgatgc tgctttacta ctatctggta aaacacaaag gctggagggg
     5341 cgatgttatc cgcaatattg ccacaaccca cgtactggac gatatggcag ccgatttcgg
     5401 atataaatgt catgaagtgc ccgtaggttt caaacacata agtgcaaaga tggaagagac
     5461 cgatgccgtt atagggggag agtccagcgg aggattgact gtaagaggtc atatattcgg
     5521 taaagacgga atatttgcgg cttccttact tatcgagatg atggctgtaa caaaaaagaa
     5581 cctgtcactg atattaaaag agatttatga cagatacgga tacagggtga tgtcggaaaa
     5641 ctcctatgcg tttaaatcgg aagacaaaga gcgacttatg aagatacttt acaaagacag
     5701 gttgattccg gattttgacg ttcctgttga aagattaagc tatgaagacg gattgaaggt
     5761 atatttcaaa aacggcggtt ggctggttgc cagattctcc ggtaccgaac cgttattgag
     5821 agtttttgcc gagatgcccg atcaacagca ggcggatgac gtttgcaaca gaatgaaagg
     5881 atttttgggt atataactat gatactggta accggaggct gcggctttat aggttcgcat
     5941 ataaccgatg ctttgattga aaagaagtat aaggtgctgg ttatggataa tttgagttcg
     6001 ggcagcatgg aaaacttaaa cagtaaagcg cagtttgtaa aaggagatat aacaaatacc
     6061 gaagatattg aagcggtttt taagaaatat ccgatagatg cggtttgtca ccatgcggct
     6121 cagatttccg ttgccgtatc catggaaaac cccgtatttg acatcaatac caacataata
     6181 ggtatcatca atcttttgca ggcatgtgaa aaatataagg ttaaaagatt tgtttttgcc
     6241 agcagtgcag caatttacgg aaatcccgta tatcttccca tagatgaaaa tcaccccaag
     6301 aatccgttgt ccttttacgg attatccaaa cttgcttcgg agcagtgcat aaggatattt
     6361 gcccaagata ccgatatgac atatgcgata ttgcgatatg ctaacgttta cggtcccaga
     6421 cagcgggcgg aaggtgaagg cggtgttgtt gcggtcttta tgaacaggat ggtggcaaac
     6481 aaagaatgct atattcacgg tgacggaaaa cagacaaggg actttatata tgtaaaagac
     6541 atagcggcgg caaatgtcaa agctctggaa tgccatgtaa gctttacggc caatgtttcc
     6601 accaatgtat ataccgatat agttacatta ttcaatgtta tgaaaaagga aacgggatat
     6661 gaaaaacagg ctgtgtatac gcagcccaga aaaggagata taaaagacag ttatcttaag
     6721 aataccggga tacacgagct tttatcctgg tatcccgaat atgatttaag taaaggtttg
     6781 aaggagactg ccgagtattt tctgcatgct tgatttttta aagaaaaaca ggatattttt
     6841 tacttatgtt ctgataggta caatagcaac tttgctggat atggcaatat tgtatattct
     6901 gtcggattta ttaagcctga actatattct ttccaattgt atttcggtat tttgcggaat
     6961 atccttaagc ttttttctca attcaagata taattttaaa aaaaccgaca ggatagctat
     7021 taagttcatt tatttcgcat cggtatgcat tataggttta ttggtcggat cttccatttt
     7081 tgttttcctg tatgagaatc tcgggattta taagttcata tccaaagcac tgtccgtaat
     7141 aatagcggga gtattacagt tcctgttcaa caagcatgtg accttccgat aacctgtgat
     7201 ataattcaat catgaaacta aaagcactta aaatcatctt aataatactg tttttgctct
     7261 caggcacagc gacttttctg ttttcaccgt attatgagct gaagaatata actgttgaag
     7321 aaagcgccgt taatattacc gacacactgg gtgaatgcgg tgtggttgca ggctgcaaca
     7381 tcaatcttct tttgttatca aaggggaata ttttaagcgg gcaccttgtg aaagcggaag
     7441 agcaggtgat gagtgatgtc aacatcaaga atttaagtat aaaaaggtcg ggaaggaatg
     7501 atattaccgt aagttgtcag tccaggaaac ccgtaatgtg tatatcacac gatgactatg
     7561 taatatattt tgattatgac ggaacgataa tagatacgtc acaaatgaag gcggaaaaag
     7621 caattcttat cagaggattg gatttagatt tcttttatcc gggacgtaat atattgactt
     7681 gtaattacat gttggaggat atttccgtct tatgtagcga gatagaaaag tacgataccg
     7741 caaattatac ggctatcaga acactgataa atgagataga aatgcaggaa aataacagaa
     7801 taatagttaa atatgacaac agattggata ttttaatgga tatgaccgat gatgtgaaat
     7861 acaatgcaaa tgttttatgt gccatactgt caaacatgaa tcagaacagc aaaggcaccg
     7921 tagattttac cgtttccgac actccgttct tcagtcctgc ttcggaataa cggaaaaagt
     7981 tatataaaaa ccttgacaaa agttttgttt gtaatgtata tacaagatga tataatgttt
     8041 gatataatga tgttaattat tagttaagta taattttcta catgaaaggg gagtattatg
     8101 cttcagttcg aaatggagat ggagcaattc gctcagataa aggttttagg agtcggtggt
     8161 gccggaaaca atgcaataga cagaatgatc acttcgggat taaagggtgt ggagtttatt
     8221 tccataaaca ccgacaaaca ggctttgatg aattcaaaag caactacgaa aattcagata
     8281 ggggaaaagc tgacaaaagg attgggtgca ggttccattc ccgaaattgg ggaaagaagt
     8341 gcggaagagt ccaaggatat tatcatagac cacattaaag gtgccgatat gatattcata
     8401 acagccggaa tgggcggcgg taccggtacc ggtgccgtac cgataattgc caaagaagca
     8461 aggcagctgg gaattcttac cgtatgcatt gtgaccaagc cgtttacctt tgaaggaaga
     8521 aagagaaaag agaatgccga aaaaggtata gacaaattgc agtctatagt ggatacgctt
     8581 atcattgttc ccaatgacag actgttgcag atttccgaca agaaaataac catattggaa
     8641 gcattcctta ctgcggacaa tgttctcttc aaaggtgttc agagtatttc cgatctgatt
     8701 gcggtacccg gccttatcaa tctggacttc gccgatgtca aaacggtgat gaccgatgcg
     8761 ggaatagcac atatgggtat cggcagaggc acgggagaag ataaatgcga acaggcggca
     8821 atggaagcga taaagagtcc tttactggaa acctctatcg aaggagccaa gaaacttctg
     8881 gtcaacatta ccggcggtcc cgatatggga ttgtttgaag tggatcatgc cgcaaacaag
     8941 atttacgaag ccgccgatga gtcggccgat atcatctggg gtgccacaat tgatcccaat
     9001 atgaccgatc agataatgat aaccataata gcaacgggat tcaatgatga tgtaaaggaa
     9061 tataaacctt cgattctttt cccgaaaaaa gaagagaaga catttacctt taaggatgtc
     9121 aaggaaccgg aaaaagaaga tatcaaaccc gaagaaccgg aagaagagaa aactgaagaa
     9181 gaagcagatg acttaaaaga aaccaggtta ttcgatattc atctgcccga cgaggaagat
     9241 aagcaaacgg atgaggagtc tgataccgtt tccaaagagg ttgaggacaa gaaacccgaa
     9301 cccgtggcac agccggaaaa gaagcagagg gatgatacca taaccattcc cagtttcttg
     9361 cgtatgaagt aaaattatga gatacatata aaagaatgcg gaaaccccgc attttttttg
     9421 ctgctttttt aataataatt tacatatagt cttaatttgt aaacttttta tgacttggtg
     9481 ataaaaagta ttgcagtatt cggaatgttg atatataatg gtagaaattg gaggtaacaa
     9541 aaatggcaat aggtaaagaa ggagtatata aggtaagaga aataagggac atcagagaag
     9601 caattttcgg tgctgccgag atatacggag ataaagatgc gttattggaa aagaataagg
     9661 aaacggataa atatgaagct cattcattta acgatttcgt aaaggatata aagagtttag
     9721 gtaccgcatt atgtgatatg ggacttaaag acaaaagagt gattgttatg ggagaaaaca
     9781 gatatttctg ggctgtttct tacatggcgg ttgtatgcgg aacgggtgtt gttgtaccgg
     9841 tggatgttct gcttaatcag actgatttga aatatatatt tgaatcatcc aaaccggaag
     9901 cggtcatatt taccgataga aaagcaaccg aaatcagaaa tctggcggag gagctttcat
     9961 acatcaaata tttcataaat atggacggag attataccga taacaaacgt tttttcaacg
    10021 ttaccgacct tatggcaaaa ggaagtaaac tggttgaaga aggtgacagg acttttatcg
    10081 atgccgaaat agataccgaa gagacacaga ttatcttgtt tacatcggca acaacttcgg
    10141 ttgcaaaagc cgttcagttg tcacacagaa atatagcaag caatatgatg gcaatgtcca
    10201 gcatgataaa ggtatatcct accgatgtat ttttatcggt actgcctttg catcatactt
    10261 atgaatgcac atgcggattc ttatgtgcta tgtctctcgg gggagccata ggttatgccg
    10321 aagggttgag gtatattaaa aagaacctgg atgaattaaa acccagtatt attctttcgg
    10381 ttcctcttct gtttgaaaag acatacaaga atataatgaa gtcagcggag aaatcaggca
    10441 gccttaaaaa gcttgagaca tttataaaaa tagccgatac acttcgtctc ggcaagaaga
    10501 taagaagaaa aatgttttct tcggttattg ccggtttagg cggcaatatg aggctgatga
    10561 tatccggtgc agccggaata tctcctacgg tagccaaatt ttacaggtcg ataggtattg
    10621 attttctgca aggatacgga ctcaccgaat gttctccgat aatctgcctg aattccgatg
    10681 tggattttgt ggatgaatcg gcaggaaaac cgttaccggg agtggaagtt gttattctgg
    10741 ataaagatgc cgacggtatc ggcgagatat gtgctaaagg tcccaacgta atgaagggtt
    10801 acatgaatat gccggaagag aacgcaaaag catttgaagg cggatatttc cataccggag
    10861 acttaggtta catggatgaa aatgactttg tatacataac aggaaggaaa aaatcggtaa
    10921 tagtcacaaa aacgggcaaa aacatattcc cggaagaact ggagcaaaaa ctggataaaa
    10981 gcaaatatgt ggaagagtgt ctggtattcg gcgtaaccga taaggacgga gacagcattg
    11041 taacggctgc ggtttatcct gcatatcaga tatttgaaga ggaattcaac aataccgaca
    11101 agaagttcat tcatgataag ataatggaac ttgtgcagga ggccaattcg caggtgccca
    11161 catacaaggc aatcaagaaa ctggttataa gggacaacga gttcgaaaag actacaacaa
    11221 gaaagattaa acgttacggc gcaaactata aagtagatta gtattgtgcc gagataggac
    11281 attgatattt caaggattca tatgtcgtac tatagcggag gataagtgac tgtggaaaat
    11341 actttaccat taattgattt tttccacagt tatcttatgc tataatatca ttattattac
    11401 atcgggggaa aaataaatga aactcaaact gaattacaaa aggacgtttc ttgtgggact
    11461 tgctttcatg tcgatatgca tgttctggca gatgtatgat tttgtcatac cgcttattct
    11521 aaccaataaa tttcaaatga gggtggattt agcaaacgcc ataatggctt tggataacgt
    11581 tttgggatta ttcatgcttc cgcttttcgg ggttttaagt gacaaggtca atacaaaaat
    11641 aggcagaaga atgccgttta ttctggtcgg taccgcagta gcggcaatat ttaccgttgt
    11701 gctgcctata gcggcaaact ccaataatct tgtgcttttc atgattgctc tgtgtttggt
    11761 gctgatagct atggcaacat acagatcacc ggcggtagct cttatgccgg atgttaccgc
    11821 aaagcccgta cgatcgcagg gaaatgccgt cataaatctt atgggtacat tgggcggtac
    11881 tatagttttg gcactgaaga tgattatcaa gccgggtgaa ggatgggatt attccacttt
    11941 cttcatagtg gtagcttcca tgatggttat aagtgtaatg attctttttg ttaccgtaaa
    12001 cgagaataag tacagaaaag aaatggaaga cataaactac ggtgatccca taatagaaga
    12061 gcccggccct gaagtaactg aagaaaaacc ggacaaactt cccaaggatg tcaggaaaag
    12121 catgatactg cttcttgcat ctatcttcct ttgggtattc ggttacaatg ccgtcacaac
    12181 ggctttctcg cagtacgtag ttaacttctt taatttaccg gaaagtcttg ccgcaggttt
    12241 ccttatggtg gcaacggtaa ccggatttct tacctttcta ccggtttctt atctgacggc
    12301 aagaatagga cgaagaaaga ccatattggg cgctttggct ttaggaataa ttctgtttac
    12361 catatcgttt tttattaagg aatttacctt ctttgccaat ttcctttttg caggtgtagg
    12421 tgttgtggca ggctccatta ccgttaatac actgcctatg gttctggaaa tgagtaaagg
    12481 ttccacaata ggaagatata caggctatta ttacacatgt accatgagtg cacagatact
    12541 tactcctgta ttaatgggaa gactgttcta tcttatcggt tatcagattc tgttcccgta
    12601 tgccacactg ttccttattc tttccttcat aaccatgctt ttcgttaagc acggagacag
    12661 taagccccag gttaagaaaa ataaattgga acagtttgat gtggaggatt aacaatggaa
    12721 tggttaatcg cggcggtaat gctcattatc atctggatgc ttatgatatg gccttccgac
    12781 tatatcaaag aggaaaaaaa taagcttgga aaatacctct atgctcacag aggtctgcat
    12841 aacgacgaaa aaggtatacc ggaaaattcc ttagccgcat ttgatgcggc ttgtgttgcc
    12901 ggttacggaa tggaactgga tatacagttt tccaaagaca tgcagattgt ggtattccat
    12961 gatgacgatt taaaaagaat gtgcggagtg gataagaagg tcaatgaact gacttacgag
    13021 gagctttcgc agttatcgct taaaggtaca caggaaagaa ttcctctttt taccgaagtc
    13081 ttgaagcttg ttgacggcag aactccgctg gtggtggaat taaagacctg caggaagtat
    13141 aaagagctta ccgaagagac atataaaata ttgaaaagct acaagggcga ttattgtgta
    13201 gagtccttta atcccatgat tgtccgtaaa tactctaaat tggatcccac tgtcttaagg
    13261 ggcctgttaa tgaccaatta tattaaattc ggaggagtta acaaggtatt gggttttctt
    13321 cttcaggaga tgcttttcaa tttcattgcc cgtccgcagt ttattgcctg tgaccataga
    13381 gcattgagta tatggggtgt aaatgttgtc aggttcatat tccgaccggt tatggcaaca
    13441 tggaccattc gcaatgagga ggaattcgag aaggttaaga gtttcgatat agttatattc
    13501 gaagactttc ttcccgatcc tgaaagatgt gagttgaatt aagtataata ttgaagatta
    13561 gtgtggttgt agaaggagtg ccgcgaatga ataagaagat attgattttt ctgttatgta
    13621 taacagttat attgttactt gtatcttgtg ataaaccgag aaacaatcaa gaaaacaaca
    13681 accctattgt tgcagagaat atagatgagc aaggcaataa ccgtatagtc ataaaaagaa
    13741 tagacggaag cagtcctctg aaaatgtcca
//
LOCUS       DAOSOM010000045        13572 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_4560_length_13572_cov_5.486720, whole genome shotgun sequence.
ACCESSION   DAOSOM010000045 DAOSOM010000000
VERSION     DAOSOM010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 13572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13572
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4560_length_13572_cov_5.486720"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PLF14_08570"
     CDS             complement(<1..391)
                     /locus_tag="PLF14_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71374.1"
                     /translation="MKNLLRRIAHSLSYTFTILIRKAVKSIAVVLSIAAFTVLICLLE
                     FTIHKQEQLLDKLYKKFDIDIIIMDSAGAYPDNLELDRLYASVFMNENNILGKNTDNL
                     KIRSSGKETISVTNEYGEVITEISVVGL"
     gene            complement(388..1812)
                     /locus_tag="PLF14_08575"
     CDS             complement(388..1812)
                     /locus_tag="PLF14_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT71375.1"
                     /translation="MTADNKSPFKVGEKYIVYLMCNEYYPAHKKINGRVDKSGEDFYK
                     YEEMVEYSSATMKEEFGEFKEGKPFYKVTNPDLHMAQKIDTTAYDFIEKEQGIWAKRA
                     EKCAITQQSAELILTNSMRSMLMFNTNDAYIVEGRTIAEEEYANGANVCVVSASFARN
                     NKLKIGDKLPLKVYENDFLPYGIMLKKGTPGVSGFLNVEGKDRIDVWLSKGFDPDRGF
                     VGEVEYEIIGTFSTRDIVNRNEFTINNNTIFVPQKSIEGDFNTRPTVHTIKRYTDKLR
                     HTELLRTSIPGCFSVIIKNGYADAFEKEMEAAGYGGIFYYFDQNYSEVKKVLDEYNNT
                     SRLIALISLLAWVTVVTVFLTVYASKSRRDIAIMRSLGTSQAKTYISQLVIIVLIVLI
                     AAIICSIVASRIYDNITAAAFEEVSKSSNLVGGLLSVTEKSKGTVITTAVAQFFVIVI
                     AFALIMLIQIKRNIMVLMKKAKKQ"
     gene            complement(1936..2343)
                     /locus_tag="PLF14_08580"
     CDS             complement(1936..2343)
                     /locus_tag="PLF14_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71376.1"
                     /translation="MIYSFKSFYRTPIRSIIYILLLAIAATSLCTSLAMWKYSAESVE
                     YVQDTFTTIGVLSELDFMEQSDTRSLSPQYDYSMLAEVKKKAMESKYVSSYDIREYIM
                     GYNENICADVKQGFYENTYPYSLSIVTGVCESM"
     gene            complement(2353..3402)
                     /locus_tag="PLF14_08585"
     CDS             complement(2353..3402)
                     /locus_tag="PLF14_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71377.1"
                     /translation="MVKNKIRILLFILVFLILTGCNRGTELNPKEKISETPAITDNTD
                     IQDEPSIKYKTADIESLIDILNEQYEKILVQPFKCCTSSSEPIAPVSSMYDGSGNIYG
                     RILSKIRFIQGLVAARGNIISINIIEDMTFDNGGYVFGNSRSYNNFFERYTCKGYTMS
                     IYKYKNCYVVFEFFNFCLPLIDKVDFMEEFAAVLEENYADKDKPMFIDEEGFYIPKEH
                     KPEFLDKFKNEFNVLYKENITSKFQEYTQSAIPLFEIDKESSSTLAEVTYNQSIIQDN
                     SVIVTLVEDNTSDNGGFVYTTDLTDNASLVAVYRYDKLYVIFELHGESFSEIGEQTDE
                     TVVRSVAKILSDLEK"
     gene            3640..3855
                     /locus_tag="PLF14_08590"
     CDS             3640..3855
                     /locus_tag="PLF14_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007147611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HOT71378.1"
                     /translation="MPITATELKNNLDKYLQLAASEDIFITKNGKIVAKLSNPYQDRV
                     DIAKSLFGIIPANHTLEEAREEQLKRI"
     gene            complement(3937..5292)
                     /gene="radA"
                     /locus_tag="PLF14_08595"
     CDS             complement(3937..5292)
                     /gene="radA"
                     /locus_tag="PLF14_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOT71379.1"
                     /translation="MAKNNNYFVCTSCGYETVKWMGKCPSCQEWNTFEEVSEVKSSKT
                     DKVREIELDTVFDIDYNESSRISSGIEEMDRVLGGGIVSGSVILVGGDPGIGKSTILL
                     QLCQHVQTDKDILYISAEESISQLGLRAKRIGLKRSNVKLASETDTDVICKAIIDTKP
                     AVAVVDSIQTVYSDRIPSIAGSVNQIKNSALMLVNAAKRSGTSLFIVGHVTKEGNIAG
                     PKLLEHMVDTVLYFEGESGSSFRILRSVKNRFGSTNEIGVFEMSNTGLTQVANPSAFM
                     LEGRNMNAPGTVVLPLLEGSRTLMVEIQALVCPTNFSMPRRMASGIDQYRINMLMAVM
                     EKNTGMRLYNYDAYINVAGGIKVNETASDLAVIVAIASSYKGKKAYEQCVVIGEVGLT
                     GEIRSVNGMDKRINEAVKMGYTKAIIPKVNLKAVSQFKDKIEIIGVENVAKAIEASLS
                     K"
     gene            complement(5303..5761)
                     /locus_tag="PLF14_08600"
     CDS             complement(5303..5761)
                     /locus_tag="PLF14_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HOT71380.1"
                     /translation="MARLSDIIEDFIKDMIDETDGQVEIQRNELAGYFNCVPSQINYV
                     IATRFNSERGYFVESKRGGGGHITISRMSTDKPYDYIMHIILSIGDEISKHSADINID
                     NVADLELISEEEKNLLKAAINDNSLKSVPVFLRDKVRADILKNMLMKTII"
     gene            5894..6463
                     /locus_tag="PLF14_08605"
     CDS             5894..6463
                     /locus_tag="PLF14_08605"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015550325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOT71381.1"
                     /translation="MAKLYFRYGAMGSSKSANAIMVVYNYKERNQTAVLLKPVSDNRD
                     GEQINVSRCGLSMECKYAETFLNQSDEEISKYDCIVIDEAQFLTKAQVETLVHIADDL
                     NIPVICYGLRADFQGKLFEGSLYLLSWADTIEEIKTICWCGKKATCNARIGPDGKIVR
                     EGPQFLLGGNDTYIPLCRKHFRSGQTKEE"
     gene            complement(6891..8963)
                     /gene="fusA"
                     /locus_tag="PLF14_08610"
     CDS             complement(6891..8963)
                     /gene="fusA"
                     /locus_tag="PLF14_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015908219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOT71382.1"
                     /translation="MKNYTTTNIRNVVLLGHGSSGKTTLAEAMLFLSKGIDRFGNIND
                     GNTTCDYDPEEIRRKFSINTSIAPVLWKNHKLNILDTPGYFDFEGEVKCATRAAEIAL
                     ILLTAKNGIEVGTEKAWSYADEVDISRAFFVTKLEEEHADFFKVVEDLKEQFGNKVLP
                     FHFPIMEDGEFKGIIDIVYKKAYIVKDKVTKVTDIPAEYLAKTEELREPIMEAVAETE
                     EELMEKYFEGEHFTDEEIIKGLTHGIEEDSIFPVFCGSSMLLAGIDVLMDELISLFPS
                     PAAYKVHAVKAGTDEQVVLNPSSDEKFAGLVFKTMVDAFVGKISLIKVCSGELKSDMT
                     VINSRTMDTEKMSNLFFIKGKKQEQTEKAVAGDIVAVAKLTDTQTNDTLSLQSFPLEL
                     KKIDFPQPSISLAVEPLAKGDEDKIGSGLSKLSDEDPTFRVENNPETKQLLISGLGEQ
                     HIDVITSKLKAKYGVSVKLTDPKIPYRETIKKKLETEGKHKKQSGGHGQYGHVKVIFE
                     PGEEEDLVFEEKIFGGSVPKNYIPAVEKGLRECIVKGVLAGYPMVKLKATLIDGSYHE
                     VDSSEMAFKIASSLAYKKLVDASPVLLEPIMSCEVMIPDSYLGDIIGDINKRRGRILG
                     MHPADKGYQKVIAEVPHAEMFKYATDLRSMTQGRGSFTMKLERYEEMPGNLAEKVVAA
                     SKREGEEE"
     gene            complement(8964..9605)
                     /locus_tag="PLF14_08615"
     CDS             complement(8964..9605)
                     /locus_tag="PLF14_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOT71383.1"
                     /translation="MQAKKRLSEAVMRRLPRYLRYFTELKENGITKISSSELGEKMGM
                     TASQIRQDFSYFGGFGQQGYGYSVEVLYDSMKRILLKEQGFTVIIIGAGNLGTALAKY
                     NNFHKRGFDIIGIFDINPKLIGKTINGTEIMSIDRLEDFIAQHETDIAILSLPRSETK
                     AIAARVANAGVKGLFNFSPMDLKLPHEIPIENVHLSDSLMVLGYKIKNFQEEK"
     gene            complement(9688..10494)
                     /locus_tag="PLF14_08620"
     CDS             complement(9688..10494)
                     /locus_tag="PLF14_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71384.1"
                     /translation="MTDKLFIRYNKVMQKVFEKKMDEVTTKEQYFSVITQAREQFEKE
                     TIDGLSVERSLRNDIEKEGIDNVLDMALTVCDMYLPYSLITILHESIGVEGIKHKILD
                     ETRPEAERASLINALTGYETEDITTFLIGYITSVHSDLLKEEASDVLATFNKDTVYSR
                     ISDIMSKKQGNEDLLSVLASMFRQSDKSDSVYRMLREHFLSAEDKGLVANIMADLDNA
                     KAVVFLRGYLSRNINDIGKSEIADICSAISRMGGNTEDFMKYIPDTASLT"
     gene            complement(10491..11021)
                     /locus_tag="PLF14_08625"
     CDS             complement(10491..11021)
                     /locus_tag="PLF14_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71385.1"
                     /translation="MKTFIKNASRILFAYTIAAAVFTFFVIAPLNMANNTTLWLLAYS
                     FVMFLFMFFIVSRTALSVGKSERYDPDAHPFFAKGFIYGLIAMVPYLILGLVHYLIYD
                     NSLELGLRIFHYLFRCALGPMYFIINTLKYKWYAFAVAYSVVPIISGIWYFMGLKGID
                     KPELKRKLKEDEDFLK"
     gene            complement(11032..12744)
                     /locus_tag="PLF14_08630"
     CDS             complement(11032..12744)
                     /locus_tag="PLF14_08630"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOT71386.1"
                     /translation="MKNLKTIIIIVLALAVAVAAYFIIDDIISKTRVENPKITEVITP
                     ANIKSDDIKRYTFCISGEQTTVELMEVKVKDEEGEEKTEMQYRLVNEPERELNQNIST
                     ALVQAASLISVDVIEESPKNLAKYGIDYNSYFEVTLKDGTNYKVYFGDVIDVTYNVYA
                     MREGVDKVYTISDTSFGMLTIYREYLLSEVIFPGSANSLTSFSLYKKGELEFTLEPEE
                     HIKWKLVEPLSAKTYSQTAQEMVDKTYEMVIGEYVNVLPTEADYRNYDLLKPAYSIKV
                     TADNKTVTLHIGRQSIEDRSYYAKFDNSDEVFLVPTKYLGWIDTELLEILYPFPYEPL
                     IKNIKSMQYVFGTGEVYNLEIREREYLMDEKLVENYEYTINGDIVELQYGADLYNYTF
                     YTTVIVDYDLQWKGPSETQKPYFLIEIDYVSGNTEKLAYYIRDNKTMYYIRYNPQFDM
                     MSQYTGTIVDSGPIEDSMHKFLALYTDYLYVLCKDGKEHDFGDWIIVKEATEEEQGLK
                     EKVCKVCGYKIAAPISVIKHTDPGSYKGANTWIYVTLGVLAAAGVVVYLLVRRHKKKK
                     AEEQ"
     gene            complement(12745..>13572)
                     /locus_tag="PLF14_08635"
     CDS             complement(12745..>13572)
                     /locus_tag="PLF14_08635"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HOT71387.1"
                     /translation="ARDVMELNGYDVSSFDLRANMEIPADANILLIVNPTIDLLEEEV
                     KLLDDFMNKGGSMMICLGAQETNEKYPNIQKFLDFYNIQYNYDRIKEYDTNSYMASNQ
                     YYIFPEIVGSEATLNVYGNISYLLTPNARSIRLLNKSKSFLAVNPLLRTSSLSKQEAS
                     VIGYDSTSGAAYIGASARSLATECRLVVLGSTDFMRDYILVNNKAYEADSSKFFIGLL
                     NWLEGDYSRVNIEEKNYFVNILSITASQANFVAVLLYVLPALILLTGGIVYLKRRHL"
BASE COUNT         3745 a         3012 c         2353 g         4462 t
ORIGIN      
        1 tcagacctac cacgcttatt tccgtaatca cttcgccgta ttcattggtg acggatattg
       61 tttcttttcc gctgctgcgt atctttaaat tatcggtatt cttacccaat atattgttct
      121 cattcatgaa cacggaagca tataacctgt caagctcaag gttgtccggg tatgcaccgg
      181 ctgaatccat gattataatg tcaatatcaa acttcttata caacttgtca agtaattgtt
      241 cctgcttatg tatggtaaat tccagaagac atataaggac ggtaaaggcg gctattgaaa
      301 gaacgacagc tatactttta acagcctttc ttataagtat agtgaatgta taagataaac
      361 tgtgtgctat tcttctcaat aagtttttca ttgcttcttc gctttcttca taagtaccat
      421 aatattccgt ttaatctgta taagcattat caaggcaaaa gcgataacaa ttacaaagaa
      481 ctgagcaacc gccgttgtta taaccgtacc tttggatttt tccgttacac ttaacaaacc
      541 gccaaccaaa ttgctgcttt tactgacttc ctcaaaagcg gcagcggtaa tattatcata
      601 tatcctgctg gcgactattg agcatataat agcggcaatc aacacaatca acactattat
      661 aaccaactgc gatatatatg tttttgcttg ggatgtaccc aaagaacgca taatagcgat
      721 atcccgtctg cttttggaag catatacggt caggaaaacg gtaaccaccg ttacccatgc
      781 tagtagggat ataagtgcaa tcaatcttga agtattattg tactcatcca gcactttttt
      841 aacttccgaa taattttgat cgaaataata gaatatacct ccgtatcccg ctgcctccat
      901 ttctttttca aatgcgtcgg catatccgtt tttaataatg acggaaaaac aaccgggtat
      961 actggttcgt aataattccg tgtgtctgag cttatcggtg tatcttttta tggtatgcac
     1021 ggtaggtcgg gtattgaaat ctccctctat ggatttttgc ggaacaaaaa tcgtattatt
     1081 gttaatggta aactcatttc tgtttactat gtcccttgtt gaaaatgtcc ctattatctc
     1141 atattcgact tctcccacaa aacctctgtc cggatcaaac ccctttgata accatacatc
     1201 aattcggtct tttccttcaa cattcaaaaa tccgctaacc ccgggtgtcc ctttttttaa
     1261 cataataccg taaggaagga aatcgttttc atataccttg agcggtaact tatcccctat
     1321 tttcaactta ttgttccttg caaaagaagc actgacaacg catacatttg ctccgtttgc
     1381 atattcctct tcggcaattg ttcttccttc aacaatgtat gcatcgtttg tgttgaacat
     1441 caacatgctt ctcatgctgt ttgtcagaat caactccgca gattgctgag ttattgcaca
     1501 tttttccgct ctctttgccc atatgccttg ctctttttca ataaagtcat aggcggttgt
     1561 atcaatcttt tgtgccatat gcaaatccgg attagtaact ttataaaaag gtttaccttc
     1621 tttaaactct ccgaattctt ctttcattgt cgccgaactg tattccacca tctcttcgta
     1681 tttatagaag tcttctcccg atttgtctac acgaccattt atttttttat gagcaggata
     1741 atattcattg cacatcaaat acactatgta tttttcaccc actttgaacg gacttttatt
     1801 atccgctgtc atgtatgttc ctgctatttc cacatattgc ggattcccaa accttttaat
     1861 atatgcgggt aatatattaa ggtcactgta atctattttt ataattactt tataaccgac
     1921 cgtaacctta ttccattaca tgctttcaca tactccagtt acaatggaca aagaatacgg
     1981 atatgtgttt tcataaaaac cttgcttgac atccgcacaa atattctcgt tataccccat
     2041 tatatattca cggatatcat aagatgacac atacttagac tccatcgctt ttttcttaac
     2101 ttccgccaac atggaatagt catactgagg agacagcgag cgtgtatcac tctgctccat
     2161 aaaatccagc tcggacaata ctcctattgt ggtaaaagta tcttgcacat actctacact
     2221 ttccgccgaa tatttccaca tagctaaact tgtgcacaat gaggttgccg ctatggctaa
     2281 cagcagtatg tatatgatac ttcttatagg tgttctgtaa aaagatttaa atgaatatat
     2341 catgactctc ctttattttt ccaagtcgga taatatttta gcaacagagc gtactactgt
     2401 ttcgtcagtt tgttccccta tttccgagaa agactcacca tgtaactcaa aaataacata
     2461 tagtttgtca tatctgtata ctgctactaa agaggcatta tccgtcaaat cggtagtata
     2521 aacaaacccg ccattgtcag aggtattatc ctctacaaga gtaacaataa cactattgtc
     2581 ctggataatg ctttgattat atgttacttc cgctaaggta ctgctacttt ctttatctat
     2641 ttcaaataaa gggatagcgg attgtgtgta ttcttggaat ttgcttgtaa tgttctcttt
     2701 ataaagaaca ttaaactcat ttttaaattt atcaaggaat tccggcttat gctctttggg
     2761 aatgtagaaa ccctcttcat cgatgaacat aggtttatcc ttatctgcgt aattctcttc
     2821 cagtactgct gcaaattcct ccatgaaatc aactttgtct attaacggca aacaaaagtt
     2881 aaaaaattca aacactacat aacagttttt gtatttgtat attgacatgg tatatccttt
     2941 acaggtataa cgctcgaaaa aattattata gcttcgagaa ttaccgaaaa catatcctcc
     3001 gttatcaaaa gtcatatctt ctattatatt aatgctaata atgttacctc ttgccgcaac
     3061 taagccttga ataaacctga ttttggacag tattcttcca taaatatttc cactaccgtc
     3121 atacatactg cttaccggcg caatcggttc agaacttgat gtgcaacatt taaaaggttg
     3181 cacaagaatt ttttcatact gctcatttag tatatcaatt aggctttcta tatcagcagt
     3241 tttgtatttt atactcggtt catcctgtat atccgtatta tcggtaatag ccggggtctc
     3301 cgagattttt tctttgggat ttagttctgt tccccgatta catccggtta agattaaaaa
     3361 aactaaaata aatagtaata ttcttatttt gtttttaacc atgtatgtat ttccctcgca
     3421 aaactaatta tgagcgtcat cgcaattata caattactca actggaattt caacttccca
     3481 ctacaaagtg ttttgtctta gcaaagaaag gaaaaaagag agagttaaga gctaattatc
     3541 gggttgttaa aagaattcgt actaatttgt tgcatattta gtacgatttt tgtaagatac
     3601 gaatgaatat gattaattaa gaaaaaagga atttaaccca tgcctattac tgcaactgaa
     3661 ttgaaaaaca acctcgataa gtatctgcaa cttgcggctt cggaggatat tttcatcacc
     3721 aagaacggca agattgttgc aaagttatcc aacccttatc aggacagagt agatatagcc
     3781 aagtccctgt tcgggattat ccccgccaat catacactgg aggaagcgag agaggaacag
     3841 ttgaaaagaa tatgatggct gttattgata cttgatacgc tctctcaacg actatacatc
     3901 tgtagcccgt ttaaccatta caatatcctt tatattctac ttacttaacg atgcctctat
     3961 agctttagct acattctcaa cccctataat ctctatctta tccttgaatt gactgacggc
     4021 ttttagattg actttgggaa tgattgcttt tgtgtaaccc atctttaccg cttcgtttat
     4081 acgtttgtcc ataccgttta ccgagcgtat ttcgcccgta aggcccactt cccctatgac
     4141 cacacactgt tcatatgctt ttttaccttt gtaactggag gctattgcca caataacggc
     4201 taaatcgctt gcggtttcgt taacctttat accgcccgcc acattgatgt atgcatcata
     4261 gttgtaaagc ctcattccgg tgttcttttc cattaccgcc ataagcatat ttattctgta
     4321 ctgatctatt cccgatgcca ttcttctcgg catggaaaaa ttggtaggac atacaagtgc
     4381 ctgtatttcc accataagag ttctggaacc ttccagtaaa ggcaacacca cggtaccggg
     4441 ggcattcata ttccttccct ccaacatgaa ggcagaagga ttggccactt gtgttaaacc
     4501 ggtattgctc atttcaaata cacctatttc attggtggaa ccgaacctgt tttttacgct
     4561 tcgcaatatc ctgaaagaag aaccgctttc tccttcaaaa tacagtaccg tatccaccat
     4621 gtgttccaaa agtttgggac cggctatatt gccctctttg gtcacatgcc ctactataaa
     4681 cagtgatgta cccgagcgtt ttgccgcatt taccagcatt aaagcggaat tctttatctg
     4741 atttacactg cctgctatgg aaggtatccg gtcggaataa acggtctgta tggaatccac
     4801 aacggcaact gccggttttg tgtcaattat tgccttacat ataacatcgg tatcggtctc
     4861 ggaagccagt tttacattgc ttcttttcaa gcctattctt tttgctctta atcccagctg
     4921 gctgatactt tcctctgcgg aaatatataa aatatcttta tcggtctgaa catgctgaca
     4981 cagctgaaga agaattgtgg actttcctat tccggggtct ccgcctacca gaataaccga
     5041 gcccgataca attcctccgc cgagaactct gtccatttct tcaatgccgc tgcttattct
     5101 ggatgactcg ttgtagtcta tatcaaaaac ggtatccaat tctatttccc gtaccttatc
     5161 ggtttttgag ctttttactt cactaacttc ctcaaaagta ttccattcct gacatgacgg
     5221 acatttaccc atccacttca ccgtttcata accgcaggat gtgcatacga aataattgtt
     5281 attcttggcc atagagtctc cttcagataa ttgttttcat gagcatattc tttaaaatat
     5341 ccgctctgac cttgtccctt aggaaaacag ggacactctt taatgaattg tcgtttatcg
     5401 ccgcttttaa caggttcttt tcctcttcgg atattaattc cagatcagca acattatcta
     5461 tattaatatc cgccgaatgt ttacttatct cgtctccgat ggaaagaata atgtgcatga
     5521 tatagtcgta aggcttgtcg gtagacatac ggcttatggt aatgtggccc ccgcctcctc
     5581 ttttactttc cacgaaatat cctctttcgg agttgaatct tgttgcaatg acatagttta
     5641 tctgtgacgg aacacaatta aagtaaccgg caagttcatt cctctgaatc tccacttgcc
     5701 cgtctgtttc atctatcata tctttaatga agtcctctat tatatcactc aatcttgcca
     5761 ttcattatca cctttgactt tctctgactt tattatatca aatatgatat ttttttcaac
     5821 ctctatttaa aaaggcactg cctgatggta tactatatat gcaacctttt tatatattct
     5881 ttcggaggaa actatggcta aactatattt cagatacgga gcgatgggaa gttcaaaatc
     5941 cgcaaatgca attatggtgg tttataacta taaagaaaga aaccagaccg cagtgctgtt
     6001 aaaaccggta tcggacaatc gtgacggcga acagataaat gtctccagat gcggattaag
     6061 tatggaatgt aaatatgcgg aaactttttt aaaccagtcc gatgaagaaa taagcaaata
     6121 tgactgcatt gttatagacg aagcacagtt tcttacaaaa gcacaggtgg aaacgctcgt
     6181 acacattgca gatgacctta atataccagt catatgctac ggcttaagag cggactttca
     6241 gggcaaactt tttgaaggca gtctctatct tctctcgtgg gccgacacaa tagaggaaat
     6301 aaagactatc tgctggtgcg gcaaaaaagc tacctgtaat gcaagaatag gaccggacgg
     6361 caaaatagta cgggaaggtc cgcagttcct gttaggaggc aatgacacat atatcccgtt
     6421 atgcagaaag catttcagaa gcggacagac aaaggaagaa taatcggata gttatgctat
     6481 tctattgaaa ggtataaagt gaaagtaata tatgtcttat taaaacaatt gctcgaacta
     6541 gatgtcgaat ggtattgtag tttaattatc tttgcaataa ttagcctaat tgcttacaat
     6601 cttgcatata ctgtagtaag ttttctttat agaaagatag atattgatag caaaactgta
     6661 ggaagttgta ttcattggac tataagaata ttcctttttt taattatgtg gcttattaca
     6721 aaaaacataa taaatttaat caaagttaaa taactactgt tcgcaatgaa taatatattg
     6781 caatgtaagc taaaaggaca ggttatctgt ccttttaata tatttactcc tcatagttac
     6841 atcttcatat ccttcagttg tatacgaaca taactgaaag tatgaatacc ttattcttct
     6901 tcgccctctc tcttggaagc tgcaacaacc ttttcggcca agttgccggg catctcctca
     6961 tatcgctcca atttcatggt aaaggagcct ctgccctgag tcatacttct caaatcggtt
     7021 gcatacttga acatttccgc atgaggaact tcggctatta ccttctggta tcctttgtcg
     7081 gcaggatgca tacctaaaat tcttcctctc ctcttgttta tgtcacctat tatgtcacca
     7141 agatatgagt cgggaatcat tacttcacag gacatgatgg gttccaacag aacgggagaa
     7201 gcatcgacaa gtttcttata tgccagactg gaagcaatct tgaatgccat ttccgaagag
     7261 tcgacttcat gataggaacc gtcgataaga gtggctttca gttttaccat gggatatcct
     7321 gcaagcactc ctttgactat gcattctctc agtccttttt ctacggcagg tatatagttc
     7381 ttgggtaccg aaccgccgaa aatcttctct tcaaatacca aatcttcttc ttcaccgggc
     7441 tcaaatatta ctttaacgtg accgtactgt ccgtgtcctc ccgattgttt cttatgtttt
     7501 ccttcggttt ccagtttttt ctttattgtc tctctgtacg gtattttcgg atcggtaagc
     7561 ttaaccgaaa caccgtattt tgctttaagt ttgctggtta taacatcgat atgttgttct
     7621 ccgagaccgc ttataagcag ttgcttggtc tccgggttat tctccactct gaatgtagga
     7681 tcttcatcac tgagcttact caatcccgaa cctatcttat cctcatcacc tttggcaaga
     7741 ggctctaccg ccaaagatat tgaaggttgg ggaaaatcaa tcttcttaag ttccaacgga
     7801 aaagactgca acgacagtgt gtcatttgtc tgggtatcgg tcaattttgc tacagctact
     7861 atatcacctg caaccgcctt ttccgtctgt tcctgctttt taccttttat gaaaaagagg
     7921 ttggacattt tttcagtgtc cattgtcctg ctgtttatca ccgtcatatc cgacttcaat
     7981 tcacccgaac agacttttat cagtgagatt tttcctacga aagcatccac cattgtcttg
     8041 aataccagtc ctgcgaactt ctcgtcgctt gagggattca acaccacctg ttcatcggtt
     8101 cccgccttaa cggcatgtac cttatatgcg gccggtgacg ggaataacga tatcaattca
     8161 tccataagaa catcaatacc cgcaagcagc attgaagaac cgcagaatac cgggaaaatc
     8221 gaatcttctt ctattccgtg agtcagtcct tttattattt cctcgtcggt gaaatgttcg
     8281 ccttcaaagt atttttccat aagctcttct tcggtttccg ctaccgcttc catgataggt
     8341 tctctgagct cttcggtctt agccaggtac tctgccggaa tatccgttac tttggttact
     8401 ttatctttaa caatatatgc ctttttatag acaatatcta taataccttt aaactcgccg
     8461 tcttccataa tggggaaatg aaaaggtaat actttgtttc cgaattgctc ttttaaatct
     8521 tcaacaactt taaagaagtc cgcatgctct tcttccaact tggttacaaa gaatgctctg
     8581 cttatatcca cttcatcggc ataactccat gctttttccg ttcccacttc tatgccgttc
     8641 tttgcggtaa gcagtattaa tgctatctcc gcagctcttg tggcacattt tacttcacct
     8701 tcaaaatcga aatatcccgg agtatccagt atatttaatt tatgattttt ccatagtacc
     8761 ggtgctatgg aagtatttat ggaaaacttt ctgcgtatct cctccggatc ataatcacat
     8821 gttgtgtttc cgtcgttaat gtttccgaat ctgtcaattc cctttgacag aaacagcatg
     8881 gcttccgcta acgttgtttt acctgaactt ccgtgtccca gcagtactac atttctgata
     8941 ttggttgtgg tgtaattttt catttatttc tcctcttgaa aattctttat tttatatccc
     9001 agcaccatca gactgtcact gagatgaaca ttctctatcg gtatttcatg cggcaatttc
     9061 aaatccatgg gcgagaaatt gaataaaccc ttgacaccgg cattggctac tcttgcggca
     9121 atggcttttg tttcgctcct gggtaaagat aatatcgcta tgtcggtttc atgctgagcg
     9181 ataaaatctt ccagtctgtc aatactcatt atttcggtac cgtttatcgt cttgcctata
     9241 agtttgggat ttatgtcaaa tattcctatt atgtcaaatc cccttttgtg aaaattatta
     9301 tactttgcca gtgccgttcc caagtttccc gctcctatta ttatcacggt aaatccctgt
     9361 tcctttaaaa gaatccgctt catactgtcg tacagcacct ctacgctgta accgtatccc
     9421 tgctgcccga aacctccgaa ataactgaag tcctgacgta tctgagaagc ggtcataccc
     9481 atcttttctc ccaactcgga agaggaaatc ttggttatcc cgttttcctt cagttccgta
     9541 aaatatctga gatatctcgg tagtctgcgc attactgctt cagacagtcg tttttttgcc
     9601 tgcatttctc tctccggtac aaataatcac ccgacaaatt atattaaaat aattatacca
     9661 tgtcaatcgg cagttggtgt tgtatgttta cgttaaagac gccgtatcgg gaatatattt
     9721 catgaaatct tccgtattgc cgcccatacg gctgattgcg gaacagatat cggctatttc
     9781 cgattttccg atatcgttta tattcctgga aagataacct cttaagaaaa caaccgcttt
     9841 ggcattatcc agatccgcca taatgtttgc aaccaacccc ttatcttccg ccgaaagaaa
     9901 atgctcgcgc aacattctgt aaacggaatc gctcttatcc gattgcctga acatggaggc
     9961 caataccgac agtaaatctt cattaccttg ttttttgctc atgatatcgg aaatcctgct
    10021 atatacggta tctttattga aggttgccag cacatcggat gcttcttcct tcagtaaatc
    10081 ggaatggacg gaagttatat aaccgataag aaatgttgtg atatcttccg tctcatatcc
    10141 cgtcaaagca tttatcagtg aagccctttc cgcctccgga cgggtttcat ccagtatctt
    10201 atgttttatg ccctcgactc ctatgctctc atgcagtatg gttatgagtg aatacggaag
    10261 atacatatcg cataccgtca atgccatatc caacacattg tcaatgcctt ctttttctat
    10321 atcatttctc agtgaccttt ccacgctcaa gccgtcaatt gtttcttttt caaactgttc
    10381 ccgcgcttgg gttataacgg agaagtactg ttcttttgtg gttacctcat ccattttttt
    10441 ctcaaacacc ttctgcatta ccttgttgta tctgatgaaa agtttatcgg tcatttcaaa
    10501 aagtcctcat cttctttaag ttttctctta agctccggtt tatcaatacc tttcaagccc
    10561 atgaaatacc agataccgct tattatcggc acaaccgaat atgcaaccgc aaaggcatac
    10621 cacttatact tcagggtgtt aattataaaa tacataggac ccagagcaca tcggaaaaga
    10681 taatggaata ttcttaatcc caactccaga gagttatcat atattaaata atgcacaagc
    10741 cccaatatga ggtagggtac cattgcaatc aatccgtata taaaaccttt ggcaaagaaa
    10801 ggatgcgcat cggggtcata tctctccgac ttccctaccg acagcgcggt tctggaaaca
    10861 ataaagaaca taaacagaaa cattacgaaa gaatatgcca aaagccataa tgtcgtatta
    10921 tttgccatat tcaacggagc tatcacgaaa aacgtaaaca cggccgccgc tatcgtatat
    10981 gcaaacaata ttctggaggc gttttttata aatgtcttca tccttcaatc tctattgctc
    11041 ttccgctttc ttctttttat gtctcctgac aagaagatat acaacaacac ctgctgcggc
    11101 taaaacaccg agtgtgacat atatccatgt gttggcgcct ttatacgagc cgggatctgt
    11161 gtgttttata acggagatag gggccgctat cttgtaaccg cacactttgc atactttttc
    11221 ttttagtccc tgctcttctt cggtcgcttc tttaacaatt atccaatcgc cgaagtcatg
    11281 ctccttaccg tccttacaca gaacataaag gtaatcggta tacagtgcaa ggaatttatg
    11341 catactgtct tcaatgggtc cggaatccac gattgtgccc gtgtactggg acatcatatc
    11401 aaattggggg ttatacctta tgtaatacat ggtcttattg tcacgaatgt aatatgccag
    11461 tttttcggta tttccggaaa catagtctat ttcaataaga aaatacggtt tctgtgtttc
    11521 cgaaggacct ttccattgca ggtcgtaatc cacaattacc gttgtataga aagtgtaatt
    11581 gtataagtcg gctccgtact gaagctccac aatgtctccg tttatcgtat attcataatt
    11641 ctctacgagc ttttcatcca taagatattc ccgctctctg atttccaagt tatagacctc
    11701 acctgttccg aagacatatt gcatggattt tatgttcttg ataagaggct catacgggaa
    11761 cggatagagt atttccagaa gctcggtatc tatccatccc agatacttgg tcggaacaag
    11821 gaatacttca tcggaattgt caaacttggc gtaatagctt ctatcctcta tggattgtct
    11881 gcctatatgc agtgttacgg tcttattatc cgcagtcact tttatggagt atgcgggttt
    11941 taaaaggtca tagtttctgt aatcggcttc ggtcgggagc acattcacat attcccctat
    12001 aaccatctca tatgttttat ccaccatttc ctgagctgtc tgagagtaag tcttagcgga
    12061 caacggctcc actaacttcc acttgatgtg ttcctccggt tccaatgtga actccagttc
    12121 gcccttttta tacagtgaaa aggatgtcag actgttggca cttccgggga aaataacttc
    12181 ggaaagaaga tattctctgt aaatggtcag cataccgaaa gaggtgtcgg aaatggtata
    12241 gaccttgtcc actccttcac gcattgcata tacattgtat gttacatcga ttacatcacc
    12301 gaagtacacc ttgtagtttg tgccgtcttt aagagtgact tcaaaatacg agttgtagtc
    12361 tatcccgtat ttggcaagat tcttaggaga ttcctcaatt acatccacac tgataagcga
    12421 tgccgcctga acgagtgccg tacttatatt ctgattcagc tctctctccg gttcgttaac
    12481 cagtctgtac tgcatttcgg ttttttcctc accctcttca tcttttacct tcacttccat
    12541 gagttccacg gtggtctgct caccggaaat gcagaatgta taacgtttta tatcatcgga
    12601 tttaatattc gccggagtga taacctcggt aattttagga ttttccactc tggttttgga
    12661 aataatgtca tcgattataa agtaggccgc taccgcaaca gccaatgcaa gtactataat
    12721 gatgatggtc ttcagattct tcatttataa gtgcctcctc ttaaggtaaa caataccgcc
    12781 tgttaacagg attaaggcag gcagcacata aagaagcact gctacgaaat tagcctgcga
    12841 tgcggtaatg gataatatat ttacaaagta attcttttct tcaatgttaa cccggctgta
    12901 atcaccttcc agccagttta aaaggccaat gaagaatttg gatgaatcgg cttcatatgc
    12961 cttgttgttt acaagaatgt aatctctcat gaaatcggta gaacccaaaa caaccaatcg
    13021 acattcggtt gcaagacttc ttgccgaagc acctatatat gccgctcccg atgtggaatc
    13081 atatccgatt acggaagctt cctgcttgga caaagagctt gtcctcaata acggatttac
    13141 cgccaaaaac gatttagact tgtttaacag tctgatactt ctggcattgg gggtaagcag
    13201 ataggaaata ttgccgtaca catttaaagt ggcttccgag cctacaatct cggggaagat
    13261 atagtactgg ttgctggcca tataggagtt tgtgtcatac tccttaattc tgtcgtaatt
    13321 gtattgtatg ttgtaaaaat ccaggaactt ctgtatattg ggatatttct cgtttgtctc
    13381 ctgcgctccc agacaaatca tcatactgcc gcctttattc ataaaatcat ccagaagttt
    13441 aacttcctct tccaataaat caatggtcgg attgactata agaagtatgt ttgcgtcggc
    13501 cggtatttcc atatttgctc ttaagtcgaa tgaggacaca tcatatccgt taagctccat
    13561 tacgtctctt gc
//
LOCUS       DAOSOM010000046        13447 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_4609_length_13447_cov_6.138292, whole genome shotgun sequence.
ACCESSION   DAOSOM010000046 DAOSOM010000000
VERSION     DAOSOM010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 13447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13447
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4609_length_13447_cov_6.138292"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1664
                     /locus_tag="PLF14_08640"
     CDS             <1..1664
                     /locus_tag="PLF14_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71388.1"
                     /translation="TTFNSDIVIKRSDAQEPLSMPYRYSKVLMDNRLVYNEEIVGINI
                     SSVKYGVSDTGLSLYNYKDILKNGDSKEAKAVVDSIKYCKGVTTLSGIISDKEKSKIT
                     IYEGYTADLMMESWHHYVIIPSALSDYINEELPEDEKVLFIMNPGTNGLAYFTIIGEY
                     ETKNKSDTLYLSYCGLSTVVLGGQKDISDHIDCMEIDVNEQADLTDFSYFLSKYFADV
                     NVLSQYEKRINSFNEPYSYMFVNTVDIKAIDLTEEEGFEKNIIRISRIDGKDKLEMSH
                     VYADALVNDYYKYSQYITDLNISTGRKGVDLYNRSAFSDIPSYDFTIELGGDLGSEFV
                     YCMKEQGEKWPCFHQAVTSITEIERMKKGCEITFYTDYTNKDLKEIRRDDFKESGLHM
                     EGGIKGYAIIPATIHEATCNHRSIDYHVVYMVERGKAIDPLDALRVGFKVIGYYELSE
                     GTKEQVTDNKKVREYDKDGNEDEYDTIFITYTGHNEKYIKQSVKNEHIEAITIETRSD
                     VEITELIKYLKKYFAPSDETAGYIGSKNELGLEYEYCFTVMNSIQ"
     gene            1818..2654
                     /locus_tag="PLF14_08645"
     CDS             1818..2654
                     /locus_tag="PLF14_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71389.1"
                     /translation="MKKRKKKVWISVIGIIVILTATALLVSCQSKEDYGNRIVIKRID
                     GKGSLRMPHIYVNKILSDKSLFSEDIEKFLVSSVRIRLDERDFFYGYPFGVLAKTENT
                     EEEKKIIRAIEYCKGITDIDVITNEKKDCEVTFYEGFNAAMMQDKTRDCVIMPSSVIK
                     FIKPDLAPQDRRIIVTRVEGSSIGTSNFTIIGEYTAKDKYDTIYVSYSGLARIFTSAE
                     SDVSEFVDSLEIKLFEGRDGIQLMEYLDEYFADANALEKYKGKTDRYGNPYIFKYEHY
                     PK"
     misc_binding    2914..3106
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3132..3374
                     /locus_tag="PLF14_08650"
     CDS             3132..3374
                     /locus_tag="PLF14_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71390.1"
                     /translation="MNTFLTVISITVCVIGAAVSFGAKFLVKKSNLAKKQVIKGIDDE
                     KVVESLKEQKAVMIVKLIGAAIFLPGMIVLYILLKR"
     gene            3382..6027
                     /locus_tag="PLF14_08655"
     CDS             3382..6027
                     /locus_tag="PLF14_08655"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOT71391.1"
                     /translation="MKILETKYDPKTIEERLYTFWEQNGYFKAKIDKKKKPFTIMMPP
                     PNITGQLHMGHAIDNTIQDIIIRMKRMQGYCALWLPGTDHASIATEAKIVESMREEGI
                     DKHDIGREEFLKRAWAWKEKYGTRIVEQLKKMGSSCDWSRERFTMDEGLSKAVVKAFI
                     KLYEKGLIYRGEKIINWCPVCGTSISNAEVEHEEKEGALWYIKYPIENENGFVTVATT
                     RPETMLGDTAVAVNPDDERYSKIIGKYVILPLMNRRIPVIADEYVEKDFGTGVVKITP
                     AHDPNDFEVGLRHNLQIINVMNDDATMNENAGAYEGLDRYEARKRIVEDLEKSGLIDK
                     IQKHMHNVGSCYRCDSIVEPRVSTQWFVKMAPLAKPAVDVVNEGKTEFVPSRFSKIYL
                     NWMEDCHDWCISRQLWWGHRIPAYYCDDCENITVAESMPEKCTKCNGKNIHQDEDTLD
                     TWFSSALWPFSTLGWPDNTEDLKYFYPTDVLVTGYDIIFFWVARMIFSGLEYMGETPF
                     STVLIHGLVRDELGRKMSKSLGNGVDPLEIIEQYGTDALRFSLITGNSPGNDLRFSET
                     KVEAGRNFANKIWNATRFVLMNFDEQPDFSDIDVANFENPEKWILTRLNNVISEVTEN
                     MEKFELGIALQKIYEFMWDEFCDWYIELVKPKLYDKQNQKRKEALYVLNYTLANAMKL
                     LHPFMPFITEEIYQSLADSDKSIMISDWPKVNKKYDYSECATDMDIIMDSIKSIRNKR
                     AELNIDAKMKAHIFFVSDDGHIRNTIKQGEQYFIKLANAKAVAVFADESKIPSDAISV
                     ILPGFKIFMPFKELVNVEEEIARLEKEYEKAISEMQRAKGKLENEAFVSKAPKNLVDA
                     EKEKYQKFSDLSKDIKLSLENLKCM"
     gene            6018..6968
                     /locus_tag="PLF14_08660"
     CDS             6018..6968
                     /locus_tag="PLF14_08660"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOT71392.1"
                     /translation="MHVIDAHCDTLYAMCKEKTQLQFTYDLVKSLNMPYLQFFAMYSP
                     PSLLLQEGGFDQSVKIHLQMLKLYEEMTKKYSYTEVLCKKDIIAFEKTDTDSCNTLFS
                     LLSVEGVYLSKGELSYIDYLYDNGVRCLSFTWNHSNEFAEGVNGNKGEGLTVLGRKAV
                     KKCNSLGILIDVSHASDKTFYDIAELSKKPFVATHSNSRFICAHRRNLDDEMLKTIAK
                     SGGLAGINYFSDFLVEKEQNREAGVDDIIRHIEHMASLTGTKHIGLGSDFDGVDKAAI
                     SDVSAVEEIINTLLRLNYSEQAVKDICAGNMIRILREVLK"
     gene            6965..8008
                     /gene="mtnA"
                     /locus_tag="PLF14_08665"
     CDS             6965..8008
                     /gene="mtnA"
                     /locus_tag="PLF14_08665"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013725074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOT71393.1"
                     /translation="MNSITPIEFKDNLLILIDQRKLFETEEYFVCDTVESVHFAIKDM
                     VVRGAPAIGVAAGYGMYIKALSLKDTDIKSFKKSMKKTADYLDTARPTAVNLFYATKR
                     MMDVVDNLSGTVTKPDEIITALYKEAVAIHNEDIKANKEIGKNMLSLLKNAKSVLTVC
                     NAGILATTAYGTATSAMYLAKEQGRELSVYACETRPRLQGARLTSYELNKNGVDVTVI
                     TDGMVAHLMSKKMVDAFIAGCDRVAANGDTANKIGTKGISIIAKRYNIPVYIACPTST
                     IDMNVKDGSGIVIEERDPSEVTHIGGVRIVPEGVKVFNPSFDVTDHEDITAIVTEKGI
                     AYPPFSETLKLLK"
     gene            complement(8132..8893)
                     /locus_tag="PLF14_08670"
     CDS             complement(8132..8893)
                     /locus_tag="PLF14_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004224168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT71394.1"
                     /translation="MNIYRFGTDKEKVIMLIHPALVMWDYFEYVIPILENDYHIIVPA
                     LPGYDRENPDTDFTSVEQIAGNLSKWLKEQKIETIDVLYGCSMGGSVALKMLSDNLVR
                     INNVILDGAITPYQLPKIITRLIAVRDFLMIALGKIGGLKLLEKAFATDEYGPEELQY
                     IAEVLNFVSYRTIWRTFESCNNYSMPKDIPPHNECIQYWYGDKEAKARAWDIKYMKNN
                     FPDTLFIQFENMGHASMASIYPDKMAKQLKVLLEG"
     gene            9080..10405
                     /locus_tag="PLF14_08675"
     CDS             9080..10405
                     /locus_tag="PLF14_08675"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT71395.1"
                     /translation="MLKRKATDFLNNWKSGKRKSLIITGARQVGKTFTVRMFAKEKYE
                     TFYELNFLENPELKTIFSGSLEADLILQLVRTYYADKPYVEGSTLIFLDEVQECPEAI
                     TALKFLSKDSRFDVIASGAALGMAFNQVTSFPVGYVDYLDLTALDFEEFLWAKGIEPD
                     IIDSLRKHYVDRTKVHDALHIKFLSLLNEYLILGGMPEVLQVYADTFDYVQADAVQRR
                     LHRDYISDIARFADPNSKIKSVSCYKSIPQQLSKENRKFQYGIVEKGGTARKFESSVD
                     WLDSAHFTVTANNVSKIEYPPEHFVKDNNFKLYTTDIGMLIAMYDVRLKAAVLMERDI
                     EEQSGNLLLRTAKGGLYEALAADFLYKKGYDKLYFYRNDSGTVEMEFLLPSNDGLIPL
                     EIKAGRNATPSLNRILESDDVPYGIKFSSQNVGVSGKKITLPIYMMMFI"
     gene            10569..12437
                     /locus_tag="PLF14_08680"
     CDS             10569..12437
                     /locus_tag="PLF14_08680"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOT71396.1"
                     /translation="MSVIWHKEEYTMVGYDWENISRDFDINRDIYNSIKDKTDEFIIS
                     YNDNADWKAGWGHDFHCDYCSATLAFNIQRSESYICPVCGKENRGARKDWAWTYMYRG
                     TSCTYVKYAGILYNITKKRGYIEYIRTVMNFLTDNYERFEAFAPNLRFIGRYSGTGLS
                     DAIYTMELLYGIDAAQDYFTQEELDKWHKGFFTPQADMFDLFANKIYNIPLWQKVSEA
                     NIGVFFKDKAIIDRAFYSRFGILDQLRRGVTEDGMWFETSTGYHYYGLGGVTDLLLLC
                     QRNEVFMPEHEEMMAIAEKQFMFPLKIMFINGRIANPADSGLDMDIFRYYYMYVKACA
                     LFDNPYFKQVLGYIKYKRDVPKTLDEILYGVKYEKCKQLPDFGSNNFEDSFCVMLKND
                     VTELYLKTGIKNICHAHPDVMTMELAFYGDLVSKDIGSAGYSSQIFYEWQRKTIAHNT
                     VVVNRTDQTYTPVGEGIWPEGIIEYFDDNHVIAKSKNVYECVDYIRDMKIDDNKVYDK
                     FEIKSFEELESDYVFYCKGKCVIDYNYEEVQSIGDKEGYQHLFDIKKIDNEKPVTVKF
                     DMEDKVVNVTVEPEEGSVVYVVNSYVNNFNDKRHGIIVRRKGKSGTYKVTYECVLK"
     gene            12653..13012
                     /locus_tag="PLF14_08685"
     CDS             12653..13012
                     /locus_tag="PLF14_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HOT71397.1"
                     /translation="MKNYVPSQCPICEGELIITDMHCKSCKTHISGEFYTTKLAKLDP
                     DQLEFVETFIKCRGSIKEMEKELKISYPTVKSRLELIAETMGYKDTEQKDDTRMKVLD
                     SIEKGEMTVEEALKILK"
     gene            13025..>13447
                     /locus_tag="PLF14_08690"
     CDS             13025..>13447
                     /locus_tag="PLF14_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71398.1"
                     /translation="MSKSKETERILKLLEEKKITSEEAKKLLDTLSEEYDKESSDKDN
                     AKWDNYNAPDNIFEKYEGMGEDIKDAIAEKMRGKYSDLGEDIRKSVLAGISNIKNNIL
                     ESLSSDIDNGINSDCSSAMKREYELVNGANIKIKGVNSC"
BASE COUNT         4525 a         2127 c         3010 g         3785 t
ORIGIN      
        1 caactacttt taacagcgat atcgtaatta aaaggtcgga tgcacaagaa ccgttaagta
       61 tgccgtacag atattcaaaa gtgctgatgg ataataggct ggtatacaac gaagaaattg
      121 taggtatcaa tatatcttcc gttaagtatg gggtaagcga tacaggttta agtttatata
      181 actataaaga tattttaaaa aacggggata gcaaagaagc aaaggctgta gtggattcaa
      241 taaagtattg taaaggtgta actacattaa gtggtataat ctccgataaa gaaaagagta
      301 aaataacgat atatgaagga tatactgcag atctgatgat ggagagttgg catcactatg
      361 taattattcc ttctgcatta agtgactata ttaatgagga attacctgaa gatgaaaagg
      421 tattattcat aatgaatccc ggaacaaacg gactggcata ttttacaata ataggtgagt
      481 atgaaacaaa aaacaagagt gatactttgt atctgtccta ttgcggatta agtactgtgg
      541 tacttggcgg tcaaaaggat atatctgacc atattgattg tatggaaatt gatgtgaatg
      601 agcaggccga ccttacagat ttctcatatt tcttaagcaa atattttgca gatgtcaatg
      661 ttctttctca atatgaaaaa aggataaaca gttttaatga gccttattca tatatgtttg
      721 tgaatactgt agacatcaag gcaattgatt taactgaaga agagggtttt gaaaagaata
      781 taataagaat atcccgtatt gacgggaagg ataaactgga aatgtcgcat gtatatgccg
      841 acgcccttgt aaatgactac tataaatatt cacagtatat aaccgatttg aatataagta
      901 cgggcagaaa aggagtggac ctatataatc gctcggcatt ttcagatata ccttcctatg
      961 actttactat agaattaggc ggagacttag gaagcgaatt tgtttattgt atgaaagaac
     1021 aaggcgagaa atggccgtgt ttccatcagg ctgtaaccag tataactgaa atagagagaa
     1081 tgaaaaaagg ctgtgaaata acattttata ccgactatac gaataaggac cttaaagaga
     1141 taaggagaga cgatttcaag gaaagcgggt tgcacatgga gggcggtata aaaggatatg
     1201 cgataatacc tgcaacaatt catgaagcta cttgcaatca tcgcagcata gactaccacg
     1261 tggtttacat ggttgagaga ggcaaggcta tagatccttt ggatgcattg agagtaggat
     1321 ttaaggtaat aggatattat gagttgtctg aagggacaaa agaacaagtg acagataata
     1381 aaaaagtgcg tgaatatgac aaggacggta atgaagacga gtatgatacg atattcataa
     1441 catacacggg acataacgag aagtatataa agcaatcggt caaaaatgag cacattgaag
     1501 ccataaccat agagacaaga agtgatgttg aaattacgga gttgataaag tatttgaaaa
     1561 agtattttgc tccttctgat gaaacggcag gttatatcgg gtcaaaaaat gagttgggct
     1621 tggagtatga gtactgtttt actgttatga atagtataca atagttcttc agtatgttat
     1681 attttagtct gcgactttta tccgcaaaaa tttttatttg acaaaagttg cttaatggta
     1741 tcggacagga aaagatattt tatttacagt atcgacagaa aaaccgttaa tattataaaa
     1801 ggaaagaggt aatgataatg aaaaaaagaa aaaagaaagt atggatttct gtgattggca
     1861 tcattgttat tttaacagca acagctttat tggtgtcttg tcaaagtaaa gaagattatg
     1921 gcaaccgcat agtcattaag agaatagacg gaaaaggttc tctaagaatg ccacacatat
     1981 atgtaaacaa aattctctcg gacaaatcgc ttttttcaga agatattgaa aagtttcttg
     2041 tttcttccgt aagaatcaga ctggacgaaa gagacttttt ttacggatat ccgtttggag
     2101 ttctagcgaa aacggaaaac actgaggaag aaaaaaagat catacgggct attgagtact
     2161 gtaaaggcat aacggatatt gatgtaataa ccaatgaaaa gaaagactgt gaagttacat
     2221 tttatgaagg ttttaatgcc gctatgatgc aggataaaac gagagactgc gtgattatgc
     2281 cgtcctccgt aatcaaattt ataaagcccg accttgctcc tcaggacaga agaataattg
     2341 taacccgtgt tgaaggatca tccattggca caagtaattt tacaataata ggagagtata
     2401 ctgcaaagga taaatatgac actatctatg tatcatattc cggcttggca cgtattttta
     2461 catcagccga aagcgatgta tcggaatttg tggattcatt ggaaattaaa ttgtttgaag
     2521 ggcgagacgg cattcagctg atggagtatt tagacgaata ttttgccgat gcaaacgctc
     2581 ttgagaaata taaaggtaaa actgacaggt atggcaatcc gtatattttt aagtacgagc
     2641 attatccgaa atagtttata tatagcaccg ttagttagtg attaacacgg tttgtataaa
     2701 aatctatatt aaatatacga gcggaggtta aggtgtggaa aacacgaaaa aacaggcgtt
     2761 actataatca tcatagtatc ggttgtgatt gttgcggcag ctttactgtt acgaataata
     2821 tgcaatagtt cttcagtatg ttctatttta gtctgcgact tttatccgca aaaatctttt
     2881 atttgacaaa agttgcttaa tgaatataat caaattaatg ctgtgaactg aaggagtagt
     2941 ttgtaaaacc tcacagagag aaagcggtag ctgaaaagct tttaggcaat ttgcagatga
     3001 aggtcgtcgg ggagcaaaac gtgatgcaag cgttaattgc aatcgagagt cccgtaaaaa
     3061 cggggaaatc aggtggtacc gcggaaagta tcctttcgtc ctgaaaaaat ttaaggatga
     3121 gaggattttt tatgaatacc tttttaaccg tcatttcaat aacagtgtgt gtcataggag
     3181 cagcggtgtc tttcggtgcc aaattcctgg tgaaaaagag taatcttgca aagaagcagg
     3241 ttataaaagg gattgacgat gaaaaggttg tagagtcatt gaaagagcag aaagccgtaa
     3301 tgatagttaa gttaatagga gcggctatat tccttccggg aatgatagtg ctttatatat
     3361 tattaaagag gtagtaaaga gatgaaaata ttggaaacaa aatacgatcc gaaaacaata
     3421 gaagaaaggt tatacacctt ttgggagcaa aacgggtatt tcaaggctaa gatagataag
     3481 aaaaagaagc cttttaccat aatgatgccg cctcccaata taacggggca gctgcatatg
     3541 gggcatgcca tagacaatac catacaggac ataataatca ggatgaagag aatgcagggt
     3601 tattgtgcac tgtggttgcc cggaaccgat catgccagta tagcaaccga agctaaaata
     3661 gtggaaagca tgagagaaga aggaatagac aaacatgaca taggcaggga ggaattctta
     3721 aagagagcct gggcatggaa agagaaatac ggtaccagaa tagtggaaca gttgaagaaa
     3781 atgggttctt cctgtgactg gagccgtgaa agatttacca tggatgaagg tttgtcaaag
     3841 gctgttgtaa aagctttcat taaactctat gaaaaaggtc tcatataccg cggagagaag
     3901 attataaact ggtgtccggt atgcggaacc tccatttcca atgccgaagt ggagcatgag
     3961 gaaaaagaag gagcattgtg gtacattaag tatcctatag agaatgaaaa cggatttgtt
     4021 accgtagcca caacacgtcc cgaaacaatg ctgggagata ccgccgttgc ggttaatccc
     4081 gatgatgaaa gatatagcaa gattatagga aagtatgtaa tcttgccttt aatgaacaga
     4141 agaataccgg ttatagccga tgaatatgtt gaaaaggatt tcggtaccgg tgttgttaaa
     4201 ataacgcccg ctcatgatcc taacgacttt gaagtgggat taagacataa tctgcagata
     4261 attaatgtca tgaatgacga tgccaccatg aacgaaaatg cgggtgcata tgaaggactg
     4321 gacagatatg aggcaaggaa gagaatagta gaagatttgg aaaagtcggg actcatagat
     4381 aaaattcaga aacatatgca caacgtaggc tcctgttaca gatgcgactc cattgttgag
     4441 cccagagttt ccacgcagtg gtttgttaaa atggcaccgt tggcaaagcc ggcggtggat
     4501 gttgtaaatg aaggtaagac cgagtttgtg ccttccagat tctccaagat atatttaaac
     4561 tggatggaag attgccatga ctggtgtata tccagacagc tctggtgggg acacagaata
     4621 cccgcttatt actgcgacga ctgcgagaat ataacggttg cggaaagtat gccggaaaaa
     4681 tgcaccaaat gtaacggtaa gaacatacat caggatgaag atacgctgga tacatggttc
     4741 agttccgcac tctggccgtt ttccacttta ggttggccgg ataataccga agacttgaag
     4801 tatttctatc ctaccgatgt tttggtaacc ggttatgaca taatattttt ctgggttgcc
     4861 agaatgatat tctccggact ggaatatatg ggagaaacgc ctttttccac ggttctcatt
     4921 cacggtcttg ttcgtgatga actgggcaga aaaatgtcca aatccttggg taacggtgtg
     4981 gatcctctgg agataataga gcagtacggt accgatgctt tgcgattttc tctcataacc
     5041 ggaaactctc ccggtaatga tttacgcttt tccgagacca aggtggaagc gggaagaaac
     5101 tttgccaata agatatggaa tgccacccga ttcgtgctta tgaattttga tgaacagccg
     5161 gacttttcag atattgatgt tgccaacttt gaaaaccccg agaaatggat attgacaagg
     5221 ttgaacaatg ttatttccga agttaccgaa aacatggaaa agttcgagtt gggtatagca
     5281 ttacagaaaa tatatgagtt tatgtgggat gagttctgtg actggtatat cgagcttgta
     5341 aaacctaaac tgtatgacaa acaaaaccaa aagaggaagg aagccttgta tgtactcaac
     5401 tacactttgg caaatgcaat gaaacttctt catcctttca tgccttttat aacggaggaa
     5461 atatatcaga gccttgcaga cagcgacaag tcgattatga tatcagactg gcctaaggta
     5521 aataagaagt acgattattc cgaatgtgct accgatatgg atataataat ggattccatt
     5581 aagagtatca ggaataagcg ggcggaactg aatatcgatg ccaaaatgaa agcccacata
     5641 ttctttgtat ccgatgacgg gcatatacga aatacaataa agcaaggtga gcagtatttt
     5701 attaaacttg ccaatgccaa agcggtagcg gtatttgcag atgaatcgaa aataccttcc
     5761 gatgccatat ccgtaatact acccggattc aagatattca tgccttttaa agagctggta
     5821 aatgtcgaag aagaaatagc aaggctggaa aaagagtatg aaaaggcgat atccgagatg
     5881 caaagagcca aaggcaaact ggaaaatgag gcatttgtat ccaaagcccc gaagaatctt
     5941 gtggatgccg agaaggagaa gtatcagaag ttctcggatt tatcaaaaga tattaagtta
     6001 agtctggaga accttaaatg catgtaattg atgcccattg cgacacactg tatgcaatgt
     6061 gtaaagagaa gactcaacta caatttacct atgacctggt aaagagtctt aatatgcctt
     6121 atttgcagtt ttttgccatg tacagtcctc cttcattgtt gctgcaggag ggcggctttg
     6181 atcaatcggt aaaaattcat ttacagatgc tgaaactgta tgaagaaatg acaaagaaat
     6241 attcatatac cgaggttctt tgtaagaaag atattattgc ttttgaaaaa acagatactg
     6301 acagttgcaa tactttgttt tcactgctgt cggttgaagg tgtttatctt tccaaaggag
     6361 agttgtcata cattgattac ctgtacgata acggtgtcag gtgcctttct tttacctgga
     6421 atcattccaa tgagtttgcc gaaggggtta acggaaataa aggggaggga cttaccgttt
     6481 taggtaggaa agcggtaaaa aaatgtaaca gcctgggtat tcttatagat gtatcgcatg
     6541 caagcgataa gactttttac gatattgcgg aattatccaa aaagcctttt gttgctactc
     6601 attccaacag cagatttata tgtgctcaca gaagaaactt ggatgatgaa atgcttaaaa
     6661 caatagcaaa aagcggcggc ttggcgggaa taaactactt ttccgatttt ttggtggaaa
     6721 aagagcaaaa cagagaagca ggtgtagatg atataataag gcatatagag catatggctt
     6781 ccttaacggg aacaaagcat ataggtttag gttcggactt tgacggagtg gataaagccg
     6841 caataagcga tgtttccgca gttgaggaga ttataaacac ccttcttagg ttgaactaca
     6901 gcgaacaggc agtaaaggat atctgtgcgg gcaatatgat acgcatactc agggaggtat
     6961 taaaatgaac agtattacgc ctattgaatt taaggacaat ttacttattc tcatagatca
     7021 gagaaaactt tttgaaacgg aagaatactt tgtttgtgat acggtggaat cggtgcattt
     7081 tgccattaaa gatatggtgg taagaggagc acccgcaatc ggtgttgcag ccggttacgg
     7141 aatgtatata aaagcacttt ctcttaaaga taccgatatt aaatcattca aaaaaagcat
     7201 gaagaagact gccgactatt tagatacagc aagacctacc gccgtaaatc ttttttatgc
     7261 cacaaaaagg atgatggatg tggtggataa tctctcaggt acggttacta agccggatga
     7321 aataataacc gctttgtata aagaagcggt tgccatacat aatgaagata ttaaagccaa
     7381 taaagagata ggaaagaata tgctgtcgct gcttaaaaat gcaaagagtg tactgacggt
     7441 ttgtaatgcg ggtattttag ccacaacggc atacggaacc gcaacgtcgg ctatgtatct
     7501 ggctaaagag cagggaagag agctttcagt ctatgcatgt gaaacacgac cgagactgca
     7561 gggagcaagg cttacatcat atgaactgaa taaaaacgga gtggatgtca cggttataac
     7621 cgacggtatg gtggctcatc ttatgagcaa gaaaatggtg gatgcgttta ttgcgggatg
     7681 cgacagggtt gccgcaaacg gtgataccgc caataagata ggaacaaaag gcatttccat
     7741 aatagcaaag agatataaca taccggtata tatcgcctgt cccacttcca ctattgatat
     7801 gaatgttaaa gacggttccg ggatagtcat tgaagagagg gacccttcgg aagtgaccca
     7861 tatcggcggt gttcgcattg ttcctgaagg tgtaaaggtg ttcaatcctt cttttgatgt
     7921 tacggaccat gaagatatta cggctattgt cacggaaaag gggatagctt atccgccgtt
     7981 tagtgaaaca ttgaaacttc tgaaatgagc acaattcatt tcattatcaa agtagtttta
     8041 atatttaata ggaaaggcaa gtaagtaaaa ctttcagtaa ctttaaagga tacatactca
     8101 atagacagta ttgcaagtat ataccttatt gtcagccttc caataagact ttaagttgct
     8161 ttgccatttt atcggggtag atgcttgcca tacttgcgtg tcccatattt tcaaattgta
     8221 taaacaatgt atcggggaaa ttgtttttca tatactttat atcccatgct cgggctttag
     8281 cctctttatc gccataccaa tattggatac attcattatg tggcggtata tctttcggca
     8341 tggaatagtt attgcaggat tcaaaggtcc tccatattgt tctatagctc acaaaattaa
     8401 gaacctcagc tatatattgc aattcttcag gaccatattc atctgtggca aaggcttttt
     8461 caagaagttt taaccctcct atcttaccta aagcaatcat caaaaaatcc cttacggcaa
     8521 taagccttgt aataatcttg ggaagttggt acggggtaat tgcaccgtct aaaataacgt
     8581 tatttattct aaccaaatta tcggaaagca tcttaagtgc tacagagccg cccatggaac
     8641 aaccgtatag gacatctatt gtctctattt tttgttcctt aagccacttt gataagttgc
     8701 cggctatttg ttctacactt gtaaaatccg tatcgggatt ttcccggtca tatcccggta
     8761 atgcgggaac tataatatga taatcatttt ccagtatagg tatgacatat tcaaaatagt
     8821 cccacattac taatgcagga tgtataagca ttataacttt ctctttatca gttccgaacc
     8881 tgtatatatt cactaaaatc ctccaagaat aatacttttg atattatatc acgcttgttc
     8941 tatttgttcg gagtaaaaaa tatacttata attagaaata tgcatacatg cagaaaaata
     9001 catggaaaaa tatgcatcgg tgcatatttt tcttgaaatc cgcatgtttt tgcgaaataa
     9061 tgagggcagg aggcatgcta tgttaaaaag gaaagcgacg gatttcttaa ataactggaa
     9121 atccggaaag agaaaaagtt taattattac gggtgccaga caggtaggga agactttcac
     9181 cgtaagaatg tttgcaaagg aaaaatatga gactttttat gaactgaact ttctggagaa
     9241 tcctgagctg aaaactattt tctccggttc attggaagcg gacttaatat tacagctggt
     9301 aagaacatat tatgcggata aaccgtatgt tgagggttct accctcattt tcttagacga
     9361 ggttcaggaa tgccccgaag caataaccgc cttaaagttt ttgtcaaaag attcccgttt
     9421 tgatgttatt gcaagcggtg ccgctttagg tatggctttt aatcaggtta catcctttcc
     9481 ggtaggatat gtagattatc tggatttgac ggctttggac tttgaggaat ttctttgggc
     9541 aaagggaata gagcccgata tcatagattc tcttcgcaag cattatgttg acagaaccaa
     9601 agtacacgac gctttacata ttaagtttct ttccttgctg aacgaatacc ttatactggg
     9661 cggtatgccg gaagttctgc aggtttatgc agatacattt gattatgtgc aggcagatgc
     9721 ggtacaaaga cgcttgcatc gtgattacat atcggatata gcgcgatttg ccgatccgaa
     9781 ctcgaaaatt aaatcggttt cctgctataa atccattccg cagcagcttt ccaaagagaa
     9841 tcgtaaattt caatacggca tagtggaaaa agggggaaca gcgaggaaat ttgaaagcag
     9901 tgtcgattgg ttggatagtg ctcattttac ggtaaccgcc aataatgttt ccaagattga
     9961 gtatccgccg gaacattttg taaaggacaa caacttcaag ttgtatacaa ccgatatcgg
    10021 aatgttgatt gccatgtacg atgtcagatt gaaagcggct gttttaatgg aaagagatat
    10081 tgaagaacaa tcaggtaatt tactgctaag aacggcaaaa ggcggtctgt atgaagcttt
    10141 agcggcggat tttctttaca aaaaaggata tgataagctg tatttctacc gcaatgattc
    10201 cggtaccgtt gagatggaat ttttattgcc ttcaaatgac ggcttaatac ctttggagat
    10261 taaggccggg cgtaatgcta caccgtcatt aaacagaatt cttgagagtg atgatgttcc
    10321 ttacggtata aagttttctt cacagaatgt aggggtatcg ggaaagaaaa taacgcttcc
    10381 tatttacatg atgatgttta tataacacaa tatttgtatg attagattct tttattgtac
    10441 ttatctgata tatataaacg gatgtctgaa tattaattga gaactttttg tgaactttgc
    10501 attttgatgt tgaaaccgtg aatgacctct gttatagtag agccgttgcg actgttagag
    10561 gcaactttat gtcagtaata tggcataagg aggaatatac catggtaggg tatgactggg
    10621 aaaacattag cagagacttt gatatcaatc gcgatatcta taactctatt aaagataaaa
    10681 cagacgagtt tattatttca tacaatgaca atgcggactg gaaagccgga tggggacatg
    10741 acttccattg cgattattgt tccgccacat tagctttcaa tattcaaaga agcgaatcat
    10801 atatttgtcc ggtctgcggt aaggaaaaca gaggtgccag aaaagattgg gcatggactt
    10861 acatgtacag aggcacaagt tgcacgtatg tgaaatatgc aggtatactt tacaatatca
    10921 caaaaaaacg cggatatatt gaatatatca gaacggtaat gaattttctt acggacaatt
    10981 atgagagatt tgaagctttt gctcccaatt tacgatttat aggtagatat tccggaacgg
    11041 gattgtccga tgctatttat accatggaac tgctgtacgg cattgatgcc gctcaggact
    11101 actttacaca ggaagaattg gataaatggc ataaaggatt ttttacacct caagccgata
    11161 tgtttgattt gtttgccaat aagatataca acatacctct ttggcaaaaa gtaagtgaag
    11221 ccaatatagg ggtattcttc aaagataagg ccattataga cagggctttt tattcccgat
    11281 tcggaatact ggatcagtta agaagaggtg taaccgaaga cggcatgtgg tttgaaacaa
    11341 gtaccggcta tcactattac ggactgggcg gtgtgaccga tcttctgttg ctttgtcaaa
    11401 gaaacgaagt atttatgccg gaacatgagg aaatgatggc tatagcggag aagcaattta
    11461 tgtttcctct aaagattatg ttcataaacg gacgtattgc aaatcctgcc gacagcggat
    11521 tggatatgga tatattcaga tactactaca tgtacgttaa ggcatgtgct ctttttgata
    11581 atccctactt taagcaggtg ttggggtata taaagtataa gagggatgtt cccaagactt
    11641 tggatgagat tctttacgga gtcaaatatg agaaatgtaa gcaattaccc gatttcggct
    11701 ccaataattt tgaagattca ttctgtgtaa tgttgaaaaa tgatgttacc gaactgtatc
    11761 tcaagaccgg tattaagaat atctgtcatg ctcatcccga tgtcatgact atggaactgg
    11821 ctttttacgg cgatctggtg tccaaagata taggttccgc aggttacagt tcacagatat
    11881 tctatgaatg gcaacgtaag actattgcac ataatacagt tgttgtcaat cggaccgatc
    11941 agacctatac tcccgtagga gagggaatat ggccggaggg tattattgaa tattttgacg
    12001 ataatcatgt tatagccaaa tccaaaaatg tttatgagtg tgtggactac attcgtgata
    12061 tgaagattga tgataacaag gtatatgata aattcgagat aaaatccttt gaagaactgg
    12121 agtcagacta tgtgttctac tgcaaaggaa aatgtgttat agattataat tacgaagaag
    12181 tacagtcgat aggagataaa gaaggatacc agcatctgtt tgatattaaa aagattgaca
    12241 acgaaaaacc tgttactgtt aagtttgata tggaagataa agttgtcaat gttactgtgg
    12301 agccggaaga agggtcggtt gtgtatgtgg taaacagtta tgtcaacaat ttcaatgata
    12361 aaaggcatgg cataatagtc agaagaaaag gaaaatccgg gacatataaa gtgacgtatg
    12421 agtgtgtttt gaaatagttt taattgaatt acccggtgga accgggtaat atttttccgc
    12481 agcacaaaac atatgttcgc tttatcgcat agttaaagca aaaaattaag aaaattaata
    12541 tagaaagtta aaaaaatcaa tattatgtat tgacatatta aaaatacctt tgtatcattg
    12601 gtatataatc ttgaagaaat aagtgtctaa actatattat ggagtgaata acatgaaaaa
    12661 ctatgtaccg tcacaatgcc cgatatgcga gggcgaactg attataaccg atatgcattg
    12721 taaaagttgt aagacgcata taagcggcga gttctatacc actaagcttg caaaactgga
    12781 tcccgaccag ttggaatttg ttgaaacttt tataaaatgc agaggcagta taaaagaaat
    12841 ggaaaaggaa ttgaaaatat cttatcccac cgtcaaatca cgtctggagc ttatcgcaga
    12901 aacgatggga tataaggata cggagcagaa agacgacacc agaatgaagg tactggacag
    12961 tattgaaaaa ggtgagatga ctgttgaaga agcattgaaa atacttaaat aacgggggaa
    13021 tgttatgagt aaatccaaag agactgaaag gatattgaaa cttctggagg aaaagaagat
    13081 tacttcggaa gaagcaaaaa agctgttgga tactttatcg gaagagtatg acaaggagag
    13141 ttccgataaa gataatgcaa aatgggataa ttataatgca cctgataata tattcgaaaa
    13201 atatgaaggt atgggagaag acataaaaga cgcaatagcg gaaaagatgc gcggaaaata
    13261 ttccgatctg ggagaggata taaggaaatc ggtgttagcg gggatttcca acattaaaaa
    13321 taatattctg gaatcgctat cttctgatat tgacaacggc atcaattccg actgttcttc
    13381 cgcaatgaaa agggagtacg agctggttaa cggtgcaaat ataaaaatta agggtgtaaa
    13441 cagttgt
//
LOCUS       DAOSOM010000047        13265 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_4706_length_13265_cov_5.136412, whole genome shotgun sequence.
ACCESSION   DAOSOM010000047 DAOSOM010000000
VERSION     DAOSOM010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 13265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13265
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4706_length_13265_cov_5.136412"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="PLF14_08695"
     CDS             complement(<1..433)
                     /locus_tag="PLF14_08695"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71399.1"
                     /translation="MDKDCAYKREDNLVYLYLEIDNRRCTEKETKILKQYAGVKYGET
                     ISRYIVMPQDMHLEALHYAIQRLFGWIDRYRYAFKLPDDVFYSITKDNVCKWASLTGD
                     LFAYIWSDNEAGYFNPLFQDNKEVDIEEFFAQQYTGPYIAGY"
     gene            complement(458..1339)
                     /locus_tag="PLF14_08700"
     CDS             complement(458..1339)
                     /locus_tag="PLF14_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71400.1"
                     /translation="MSQIQKDALICYIDEALPLIDTEIDQSMKIKKDLQELCRGFSKN
                     YKFNTILVSIPILVLILLVITMMIISLNIVVGIDKLLGIPNDSISIVVDVLPIIATHV
                     YLFLLLLRRIVKTSFVIEAQNYINKIEYIIRLLNENTERLNALKNRLNSNCDHREREQ
                     GFINYLKLLGYREGSYDVSQSLYQISTRHEEMKKKMEKSYYDIPIIICYFFALLSLLP
                     ASAHRTYMYFFNRNIIYDYDTYYTVFFIIVSFLLILANLIITNKEIKLSIWTFLISVV
                     FLLVIIAVLILYVVFTQ"
     gene            complement(1452..2405)
                     /locus_tag="PLF14_08705"
     CDS             complement(1452..2405)
                     /locus_tag="PLF14_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71401.1"
                     /translation="MAGIKEFDDNILQEFEKDAKDIIYKHLVEFKDLSNKLREENGKY
                     ILKARVIISFIFYILLLSVVILFAFFIPNIVYNLNLAEESLIIFGIVALYGCFAYFSV
                     LLIERILKCLHYYKIFLLTNEIENLIEDIKKTIAGLPAIREDIIASIKKNQRVTVSSV
                     AQNKNVMYTNINDFKKRKERLIYSKPGFLDGAEKSLYWIACITLLSVLSAIGPSIGLM
                     FFPETEDYSIFILVQFLMNHGALVALHIILHKKGIRTSFKTFLGSVLCALAGAGGALI
                     LYVLVLMVIGLFKFIWKIIVYFLLGVFFIGGLIGLLCSKEK"
     gene            complement(2454..4625)
                     /locus_tag="PLF14_08710"
     CDS             complement(2454..4625)
                     /locus_tag="PLF14_08710"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOT71402.1"
                     /translation="MTDLEVSNCTKKTSSLINTLSYNGPSTPLMTDVEFKGRNGNTYK
                     VLSDGFIHQGGESVLYKCICNNEVYVAKILNFSVSLRNDELRGPVLDFLIRHSGKDSH
                     LLQLLDYGTIRYNDGRYDALRYIEIYPFCPKGDLGRLGKIDYDTLCEKVIPAINTALN
                     TLHNANLVHRDVKPNNVYELDGEIYLGDFGLLTSSIIEDGHSNAHAYTLTQRGTEGYM
                     APEIYKGSVYNISDYYSFGQTISTLYHGKHMYEHLMNNTDYIANRDINLSLHRCMMEG
                     ILYLSLETKHKELDKLLRGLLKCNHELRFGYEHVCRWLTGDKTLTVPEDRNIDQLNPP
                     FMINDVKCRNIESMVEQLLLNWDMAKEYLYEGIMKDFFSAQNTELSMKALEISRKQGN
                     ELVNNTGLSQFIHYLTEGEGIYWKGRKYNDHAEIFSDYTDNHSINPDVIELLSSGFLS
                     WRMEELNKKDNKYQNQIELLKSIEKECFCYSSIGYHYARHKLRGEEISYSDCQNVAEL
                     IDYFIESFGKTYFTGETWLRDDRFYGFLMALGHVKIATSLFEEVRWAQPANAIRALFG
                     TLEVLIERGNSTSLQTKKELLNIYLLYGPRSYLCWIKENLSCYDFMGMNAFILKEKIE
                     DINLSNKKSLADIDSAFNELAKNLNQFKLYFCDNPILCKLGISNKEGNDIIYSKSLDG
                     YFLSDFYGNKVPVGFYKYIDAYRSKKKIVKNLNNSEDLFSF"
     gene            complement(4618..5337)
                     /locus_tag="PLF14_08715"
     CDS             complement(4618..5337)
                     /locus_tag="PLF14_08715"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HOT71403.1"
                     /translation="MLHVVAITDKGPSNEKNDDCILVNNTVITEGSFYGEYRSYLLAS
                     VFDGVGGEHYGDEAAKIAATGFSTLDVKDRLSEHVIVDRIKAVNRDILDAQRTDVYHS
                     KMATTIAGVYISNDSFIAFNVGDSRVYRLRGEYMLQISKDHRYSEKVHTIYRYLGHPT
                     DYLPYTDMFDNLFFDNDILVICTDGISDFVYNSVIKRELMKVEDNYSLTECAEEIMNT
                     AIKEGCNDNLSIILIRKCEND"
     gene            complement(5342..7207)
                     /locus_tag="PLF14_08720"
     CDS             complement(5342..7207)
                     /locus_tag="PLF14_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008820356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT71404.1"
                     /translation="MDNQSVKPKWQKEIELFRGIKSAYIIEGNINDNYPICEEGGSVY
                     FANLDKTIIKLFDKDVYDFCFCNPAVGLYDPLNRSLQKETVEALIGKFNNNELCSRYD
                     DIFGHVYMIREIGLLADIIKKALVGTTNNNEGERKPVAVVVNFASKFFSDVSNINNDE
                     MKALLNILLASLNSRYYRNNVNTLILVVEKFNDLPTWFYYNNPKVKTVTIPNPDRLVR
                     TVYIEKEFTEFNSIEKAKDVIDKLVSATEGLKLIELRELRQLHMKNNDDISNINETVS
                     AYKYGIKDNLWQSFNKGTIERLERYLKERIIGQDYALNKAITIIKRAVMGMTGLQHSS
                     ADNKPRGVLFLAGPTGTGKTELAKAITVILFGDERNCIRFDMAEFSEPHSDQKLIGAP
                     PGYVGYEAGGQLTNEVRKRPYATVLFDEMDKACPTIMDKFLEILEDGRLTDGQGNTVY
                     FSGTLIIFTSNYGYTEMRIDENGNEKREWIIKPGELPYEEVRKKVVDSIKKSKAMKPE
                     IINRIGEHNIIVFDFISKEAAEKIIKKQIKQVNEDCKKQKGISMHVLDECYELLNKKS
                     RSDDVMIYGGRGIGTLVENEYINPLSNYLFDNDFTHEVKITAFVKGDKLDFREEV"
     gene            complement(7209..7790)
                     /locus_tag="PLF14_08725"
     CDS             complement(7209..7790)
                     /locus_tag="PLF14_08725"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S single cluster domain-containing protein"
                     /protein_id="HOT71405.1"
                     /translation="MNVDRIAFPIKSLGPGNRIAIWTAGCKKHCYNCITPELWEPDPM
                     KEISVQSLVHIITTVVSDNGITVDGITITGGDPFEQLEDLLNLLPYVKNMVKDILVYT
                     GYEYSQFKKTLSEWKIECIESNISVLIDGQYVDKLNDNKSALIGSTNQNIIFFDEKLK
                     SKYSEYMEKGRSIENIIHGKKVFSVGIHNREEK"
     gene            complement(8012..8377)
                     /locus_tag="PLF14_08730"
     CDS             complement(8012..8377)
                     /locus_tag="PLF14_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71406.1"
                     /translation="MKACDICGASNDDNSRICNVCGGRFAEKSVSYVYYVRCNTCNAD
                     ILVQNEKQDKVFCKTCDKELVIDGIVHSVSRRETIAEEELYVEPTLILEEVKSKHKFI
                     IPKEGGVFGRYGQFDPHFF"
     gene            complement(8378..9691)
                     /locus_tag="PLF14_08735"
     CDS             complement(8378..9691)
                     /locus_tag="PLF14_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71407.1"
                     /translation="MSGFTIGEYELARREKEWLRLIEEENRKRLKEATDRYRSCLANV
                     KSVKHRISAHMVEQEKTLEAYSYMKQPFDELKSIKKKYCEKIERLCTGNIPSEVVEIN
                     ELSDKLEEDIGVISAEFFKKTEKVEKAIREYMRIHADNIATKQVSVELDRFSKRIKEA
                     VRVDYADFIEPSSNKTEEDLVSKFAAVLQLAEECFNISHMDSVKSNVINIVTTLNSVS
                     DKTYDEKKVVLDQIYSKLVSIKKEAIAKSKRFDALYTQYLAECILAGSKPMVDYSFTS
                     LEALSDELKSVKKRAEEKAEHKYITEQINDVMRSMGYKSIDSQLLELPKVGTRQLFRI
                     KENVALNVYASENNEVNTQLIIIGDSKNITEEDYEQFEEEQRSFCKRNPELVRRLSER
                     GILIKNGKIKEQDKNDLVKEKIVTDAKSENRKSRKRKMEIRMERE"
     gene            complement(9730..11589)
                     /locus_tag="PLF14_08740"
     CDS             complement(9730..11589)
                     /locus_tag="PLF14_08740"
                     /inference="COORDINATES: protein motif:HMM:NF014581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HOT71408.1"
                     /translation="MMNRYSNEYEIERSTSRLDKESSTCGPVVYYNGKPYVYDGESHV
                     LYIGKSGKGKSRCGTISTVLSVQQAGESYIVSDPKGEIYNATYCFLKDKYIVHVIDFR
                     NILESETWNPLELAYKLYSTGDKHKMEIAYELIVELAFLLFADSVVADPFWAQSARSL
                     FIGIVYLLFSFAPADQINMKSVYMLAVLGEQRLGSTNYLRHLIDLLPKDTTASMNLNS
                     YMAAPNETRGSIKCVFLDGMAIFARSEGLIEMLSSDGLNIAEMNGEKPVAIYIILPDE
                     SIIYDRLCGVLISQLMKHYMRLAQDEYKGRLPIRMHLILEELASVGSSISGLPDLMAT
                     SRSRNIKIYIVLQSLSQLDDLYGKSNASTILANIGIIIAFSTNDWKTLEELSQKCGDK
                     EIDFGTRIVTTSLIKPEQLAGMDVKQALVMIDGSVKYITKLPFYDEIFDFSEWKEPAQ
                     LKHKKENNPKYFDITKYVQEGGETDLRVPMMLQNEDIFRTFNKPKINEEIKLQELITK
                     IDKAIERLEGTEEIEEIEELKEIEKLMELDMQEMIDEEDIPDSYDIILYRCNSCENVI
                     ALVVAKILDVSYNEAVNKLKEEPQIFKFETRIEAERAKADIRNVGGDVYNLFD"
     gene            complement(11608..12357)
                     /locus_tag="PLF14_08745"
     CDS             complement(11608..12357)
                     /locus_tag="PLF14_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71409.1"
                     /translation="MRETLLYGHEEIKKAINEICVNSKAYSIGRVRPSNYIIHLDRGN
                     GRTTLARYITEKFYKHSIIKFSSKDLFLEIDFDGTLKQLQESFGYISLAACYANSYEG
                     VLFFGIDSLGDYIHETQVELFLDNIAKVSETATLIFYVPENPSRSISQLIDKIRDVLK
                     DTVLIEKSVYTATELTLIVESMLEKRGIEIDQKAQFHEQLVNLIAHMELKTIDDVKEL
                     SKRIILLTDYSSLISRVGSSQLRKMRHDVLS"
     gene            complement(12374..12967)
                     /locus_tag="PLF14_08750"
     CDS             complement(12374..12967)
                     /locus_tag="PLF14_08750"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOT71410.1"
                     /translation="MNERFIPTIITNINGVEQITDSVNIDFMRRRIYLVGEIDSTSST
                     AMLSMLGYLSNKSSDDIELFINSPGGAIQDGLAIYDTIKYGVNCDVVTVAYGRAASMG
                     AFLLACGKSGKRYALPNAEIMIHQPLGRVQGQATDIILAADNIKKTKEHIASIFAGKC
                     NKPVDILLKDMERDYVMTSKEALSYGLIDNIGFPKSV"
BASE COUNT         3896 a         2814 c         1828 g         4727 t
ORIGIN      
        1 tgtaaccggc aatatacgga cctgtgtatt gctgtgcaaa aaattcttca atatccactt
       61 ctttattatc ctgaaataaa ggattaaagt accctgcctc attgtcactc catatatatg
      121 caaacaaatc tcccgttagc gaagcccact tgcatacatt atcctttgtt atggaataga
      181 acacgtcatc ggggagcttg aatgcatacc tatatcggtc tatccagccg aataatcttt
      241 gaattgcata gtgtaaagct tctaaatgca tgtcttgggg cataactata taccgtgaga
      301 tagtttctcc atattttaca ccggcatact gttttagtat ctttgtttcc ttttcggtgc
      361 atcttctgtt atctatctcc agatacagat aaacaagatt atcttccctt ttatatgcac
      421 agtctttatc catctatata cctccatata tcagttctta ttgagtaaaa actacataca
      481 atatcagtac tgctatgatt accaacaaaa aaactactga aatcaaaaaa gtccatatac
      541 tcaattttat ttccttgttt gtaattatca gatttgccaa aattaataaa aatgaaacaa
      601 tgataaagaa tactgtatag taagtatcgt agtcataaat aatatttcta ttaaaaaaat
      661 acatataagt acgatgagcc gatgcaggca gaagtgacaa tagtgcaaaa aaatagcaga
      721 taataatcgg tatatcgtag taactctttt ccattttctt cttcatttct tcatgcctgg
      781 tacttatctg atatagactt tggctaacat cataacttcc ttctctataa cccagtaact
      841 taagatagtt tataaaacct tgctctcttt cacgatgatc acaattgctg ttgagtctgt
      901 ttttaagagc atttaaccgt tcagtatttt cgttcagcag ccttatgata tattctattt
      961 tattaatata attttgcgct tcaatgacaa atgatgtttt tactattctt ctgagcagca
     1021 aaaggaagag gtaaacatga gttgcaatta tcggcagaac atctaccaca atagagatac
     1081 tgtcattcgg aataccaaga agtttatcaa tgcctacaac aatgttcaac gaaataatca
     1141 tcatagtaat taccagcaga atcagtacta aaatcggaat agataccagt atagtattaa
     1201 atttatagtt ttttgaaaac cctcggcata attcttgaag atccttctta attttcatag
     1261 actgatcgat ttcggtatca ataagcggta atgcctcatc tatgtaacat attaatgcat
     1321 ctttctgtat ttgggacata ttatattcct ccttcacgac tgtttaacaa actgttgctt
     1381 tcttcccttc tttaatacca atatataaaa caatgttatc tataaacagt ttaaatccgc
     1441 aatgaaagat atcacttttc cttggaacaa agaaggccga ttagtccgcc gataaaaaaa
     1501 actccaagaa gaaaatatac aataattttc catataaatt taaacagacc aatcaccatt
     1561 aaaacaagaa catacagtat tagggctccg cctgcacctg ccaaggcaca taaaacagaa
     1621 cctaaaaaag ttttaaaact tgttctgatt cccttcttat gtaaaataat atgcaatgcc
     1681 actaatgctc catgattcat taaaaactga actaaaatga atatagaata gtcttctgtc
     1741 tcagggaaaa acattaaacc tatgctcgga ccgattgcgg acaatacgga taaaagagta
     1801 atacaagcta tccagtaaag tgatttttcc gctccgtcca ggaatcccgg cttgctgtaa
     1861 atcaagcgtt cttttctttt tttaaaatca ttaatattcg tatacataac atttttgttt
     1921 tgtgcgactg aacttactgt taccctttga ttctttttaa ttgatgcaat aatatcttct
     1981 ctaatcgcag gcaatcctgc aatagtcttc tttatatctt caattagatt ttctatttca
     2041 ttagtaagaa gaaaaatttt gtaataatgc aagcatttca gtattcgttc aatcaataaa
     2101 accgaaaagt atgcaaagca accgtacagt gccacaatac cgaaaattat taaagactct
     2161 tctgcaaggt taaggttata tacaatattg ggaatgaaaa aggcgaatag aattacaacc
     2221 gataaaagaa gtatatagaa tataaagctt attataaccc ttgcttttag tatatatttc
     2281 ccgttttctt cccgtagctt atttgaaagg tctttaaact caacaagatg cttataaata
     2341 atgtctttag catctttttc aaattcctgt aatatattgt cgtcaaattc ttttattcct
     2401 gccatattta tatccttata catttcaata tttacccttc acatattgca agtttagaag
     2461 gaaaacaggt cttcggagtt atttaaattc ttaactattt tctttttact cctgtatgca
     2521 tcaatatact tataaaatcc gaccggtact ttattcccat agaagtcgga taaaaaataa
     2581 ccgtcaaggc tttttgaata aataatatca ttcccttcct tattactaat ccctaattta
     2641 catagaatag gattgtcaca gaagtatagc ttaaattgat taagattttt ggccaattca
     2701 ttaaatgctg aatcgatatc tgcaagactt tttttattgc tcaaattaat gtcctcaatt
     2761 ttctctttta aaataaaagc gttcataccc ataaaatcgt aacaactaag attctctttt
     2821 atccagcaga gataggaccg cggaccatat aacaagtaga tgtttaaaag ttctttttta
     2881 gtttgaagtg aagtgctgtt tcctctttct atcaaaactt ctaaagtacc gaataatgct
     2941 cttatggcat tcgcaggttg cgcccatctc acttcctcga ataaagaagt tgcaatttta
     3001 acatgaccta atgccattaa aaatccataa aacctatcat ctctcaacca tgtttctcca
     3061 gtaaaatatg tttttccgaa tgattcaata aaataatcta ttaattcagc aacattttgg
     3121 caatctgaat aacttatttc ttcccctctt agtttgtgtc ttgcataatg ataacctatt
     3181 gaactatagc aaaaacattc tttttcaata ctcttaagaa gctcaatttg gttttgatat
     3241 ttattatctt tcttattcag ttcttccatt ctccaggata aaaaaccgga ggaaagcaac
     3301 tctatgacat cgggattaat actatgattg tctgtataat cgctgaaaat ttccgcatgg
     3361 tcgttatatt ttcttccttt ccaatatatc ccttcccctt ccgttaaata atgaataaat
     3421 tgacttaatc cggtattatt aactaattca tttccctgtt ttcttgatat ttccagtgct
     3481 ttcatactta actctgtatt ctgagcactg aagaagtctt tcattatccc ctcgtataag
     3541 tattcctttg ccatatccca gtttaataat agctgttcaa ccatgctttc tatgtttctg
     3601 catttgacat cattaatcat aaatggcgga ttcagttgat ctatgtttct gtcttccggt
     3661 acggtcagag tcttgtctcc ggtaagccat ctgcaaacat gctcataacc gaaccttaac
     3721 tcatgattgc acttcaacaa gcctctgagt aatttatcta gttccttgtg ctttgtttcc
     3781 aaacttaaat ataaaattcc ctccatcata caccgatgaa gagaaagatt tatgtctctg
     3841 tttgctatat agtcggtatt attcataagg tgttcataca tatgttttcc atgatataaa
     3901 gtggaaatgg tctggccgaa cgaatagtaa tcggaaatat tataaacaga acctttatat
     3961 atttctggag ccatataacc ctcagttcct ctttgtgtta atgtgtatgc atgtgcatta
     4021 ctatgtccat cttctatgat gctgcttgtc aagagtccga aatcccccag atatatttcg
     4081 ccgtccagtt catacacatt attgggtttt acatctctat gaaccagatt tgcattgtgc
     4141 aaagtattaa gagcggtgtt gatagcaggt ataacttttt cacacaaagt atcataatca
     4201 attttcccta atcttcccaa atcaccttta gggcagaacg gatatatttc aatatatctc
     4261 agcgcatcat atctgccgtc attatatctg attgtcccgt aatcaagtag ttgcaagagg
     4321 tgtgagtctt taccggaatg tcttattaaa aaatctaaaa caggtcctct tagttcatca
     4381 tttctcaagc taacagaaaa atttagtatt tttgcaacat aaacctcatt attacaaata
     4441 catttgtata aaacagattc tccgccttga tgaataaacc cgtctgacaa gaccttatag
     4501 gtgtttccgt ttcttccctt gaattccaca tcggtcatta acggagtaga cggcccgtta
     4561 taacttaatg tatttattaa actacttgtt tttttagtac agttagacac ttctaaatca
     4621 gtcattttcg cacttcctta ttaatatgat acttaaatta tcgttacacc cttccttaat
     4681 tgcagtattc attatttcct ctgcacattc cgtcaaagaa taattgtctt caactttcat
     4741 aagttcccgc ttgattacac tgttgtaaac aaagtcgctg ataccatcag tacaaataac
     4801 aagaatgtca ttgtcaaaaa acaagttatc aaacatatca gtatacggta ggtaatcggt
     4861 agggtgtcct aagtatcggt atattgtgtg aactttttcc gaatatctat gatctttcga
     4921 tatctgcaac atatactcgc ctcttaagcg atacacccga ctgtcaccga cattaaaggc
     4981 tatgaagctg tcattactta tataaacccc tgctattgta gttgccattt tgctgtgata
     5041 aacatcggtc ctctgtgcat caagaatatc cctattcacc gcctttatcc tatcaactat
     5101 aacatgttca cttaatctgt ctttaacatc aagtgttgaa aaacctgtcg ctgcaatttt
     5161 agccgcctcg tctccgtaat gctcgcctcc gacaccgtca aaaaccgatg caagcaggta
     5221 ggaacggtat tctccataaa acgatccttc ggttataaca gtattattaa ctaatataca
     5281 gtcgtcattt ttctcattac tcggaccttt atcggtaata gcaacaacat gtaacacttg
     5341 actatacctc ctctctgaag tccagtttat ccccttttac aaatgcagtt atttttactt
     5401 catgggtaaa atcattatca aacaaataat tggaaagagg gttgatgtat tcattttcta
     5461 ccaatgttcc tatacccctg cctccataaa tcatcacatc atcggaccga gatttcttat
     5521 ttaataactc atagcattca tctaaaacat gcattgagat tcccttttgc ttcttgcagt
     5581 cttcgttcac ttgttttatc tgttttttaa ttatcttttc tgcggcctct ttgctgataa
     5641 aatcaaatac aattatgttg tgttccccta tcctgtttat tatttccggt ttcatcgctt
     5701 tggatttttt aatagaatca accacttttt tccttacctc ttcatagggt aactcgccgg
     5761 gtttaataat ccattctctt ttctcgttac cgttttcatc aattctcatt tccgtatatc
     5821 cgtagttact ggtaaaaatt atcagtgtcc ctgagaaata taccgtattg ccttgtccgt
     5881 ctgtcaacct gccatcttcc agtatttcta agaatttatc cataattgtc ggacatgctt
     5941 tatccatttc atcaaatagt accgttgcat atggtctttt tctcacttcg ttagtcagct
     6001 gccctcccgc ttcataacca acataaccag gaggtgcacc gattagtttc tgatccgaat
     6061 gtggttcact gaattcagcc atgtcaaatc ttatacagtt tctctcatca ccgaaaagga
     6121 ttacagttat agcttttgcc agctccgttt ttccggtacc ggtaggaccg gctaagaaca
     6181 gtacaccgcg aggtttatta tctgcagatg aatgttgcaa acctgtcatt cccatcaccg
     6241 cgcgctttat tatcgttatt gctttattca atgcatagtc ctgaccgatt attctttctt
     6301 taagatatct ttccagtctc tctattgttc ctttattgaa actttgccat aaattatcct
     6361 taataccata tttatatgca gacaccgttt cattaatatt ggatatgtca tcattattct
     6421 tcatgtgaag ttgtcttaat tctctaagct caatcagttt taatccttcc gtggcgctta
     6481 caagcttatc aattacatcc ttcgcctttt ctatactgtt aaactcggtg aactctttct
     6541 cgatatacac agttctgacc aacctgtcgg gattgggtat agtaacggtc tttaccttgg
     6601 gattattgta gtagaaccat gtgggaaggt cattgaattt ctccacaacc agtatcaatg
     6661 tattgacatt gttccggtaa tatctggaat tcaatgaagc taataatatg ttcaacaatg
     6721 ccttcatctc gtcattattt atattggata cgtccgaaaa aaactttgat gcgaaattta
     6781 ctacgactgc aacgggtttt ctttctcctt cgttattgtt tgttgttcct accaatgctt
     6841 ttttaataat atcggcaagt aaacctattt ctctaatcat ataaacatga ccgaatatat
     6901 catcataacg ggaacataat tcattattat taaacttgcc tatcaatgct tcaacggttt
     6961 ctttttgtaa acttctgtta agaggatcat ataaccctac cgcaggatta cagaaacaga
     7021 aatcatatac atccttatca aacaacttta ttattgtctt atcaaggtta gcaaaatata
     7081 cactacctcc ctcttcgcag ataggatagt tgtcatttat attgccttca attatatatg
     7141 cactttttat acctctgaaa agctctatct ccttttgcca tttgggcttt actgattgat
     7201 tatccaattt atttctcctc tctattatgt atacctacag aaaaaacctt tttcccgtgt
     7261 attatattct ctatgcttct gcctttttcc atatattccg aatatttaga tttaagtttc
     7321 tcgtcaaaaa atataatatt ctgattggtt gagcctatca atgcactttt gttatcgttc
     7381 aatttatcta catattgccc atcaattaaa actgaaatat tactttctat gcattctatt
     7441 ttccactcgc taagagtttt cttaaattgc gagtactcat aacctgtata aaccaaaatg
     7501 tcttttacca tattttttac ataaggaagt aagttcaaca ggtcctccag ctgttcaaaa
     7561 gggtctcctc ccgttatggt aataccgtca acagtaatac cattatcgga aacaacagtt
     7621 gtgataatat gcacaaggct ttgtacggat atttctttca tcggatcggg ctcccacaat
     7681 tctggcgtta tgcaattata gcaatgcttt ttacatcctg cagtccaaat tgctatccgg
     7741 ttacccggtc cgagtgattt gattggaaaa gcgattctgt caacattcat ctgtactacc
     7801 cgtttattat aactttaaaa tatatgtcgg caagccctat catctgacca tctctgattt
     7861 caacggcaat actgttcatt gacggtttaa atgtccttcc gtctatatat gtgtcattgg
     7921 tctcactgtt gggttttacc gaccatttat tactgtcact gtcataactt attacaatat
     7981 gacgccttga taccctggaa aaacgttcat cttaaaaaaa gtgtggatca aattgtccgt
     8041 atctgccaaa tactccgcct tctttgggga tgatgaattt atgcttggat tttacctctt
     8101 ccaaaatcaa tgtaggttct acatatagtt cttcttctgc aatcgtttct ctcctggata
     8161 ccgaatgtac tattccgtct atcacaagtt ctttatcaca ggttttgcag aaaaccttat
     8221 cctgtttttc attctgcact aaaatatcgg cattgcatgt gttacacctt acatagtaaa
     8281 cataagaaac agatttttct gcaaaccttc caccacaaac attacatatc cttgaattat
     8341 catcatttga cgcaccgcat atatcacatg ctttcattta ttccctctcc atccttattt
     8401 ccatttttct ctttctactt tttctatttt cactcttcgc atcggtcact attttttctt
     8461 taaccaaatc atttttgtct tgctctttta tcttcccgtt cttaatcaaa atacctcttt
     8521 ccgataatct tcttactaac tccggattgc gcttgcagaa gcttctttgt tcttcttcaa
     8581 actgttcgta atcttcttcg gttatattct tactgtcccc gataataata agctgtgtat
     8641 tcacttcgtt gttctcggaa gcatatacat tcagtgcaac attttccttt attctgaaca
     8701 actgtcttgt tccgaccttg ggtaactcaa gaagctgact gtctatggat ttgtagccca
     8761 tactacgcat aacatcattt atctgttcgg ttatatattt gtgttccgct ttttcttccg
     8821 ctctcttctt aaccgattta agctcgtcag acaaagcttc gagtgatgta aaactgtaat
     8881 caaccatcgg cttactgcca gctaaaatac attccgccag gtattgagtg tacagtgcat
     8941 caaacctttt actctttgct attgcctcct tcttgatcga aactaattta ctgtaaattt
     9001 gatccaacac aacctttttt tcatcataag tcttatcgga aacggagttc agagttgtta
     9061 cgatgttaat tacattggat ttgacggaat ccatatgtga aatattgaag cactcttccg
     9121 ctaattgcaa tacagcagca aacttgctta ccaaatcctc ctccgtctta tttgaagaag
     9181 gttcaataaa gtcggcataa tccactctga cagcttcttt aattctcttt gaaaacctgt
     9241 ccaattcaac tgatacttgc ttagtagcta tattgtccgc atgtattctc atatattctc
     9301 tgattgcttt ttccactttt tccgtttttt tgaaaaactc cgcacttatt acccctatat
     9361 cctcctccaa cttgtcagat aactcgttta tctcaacaac ctcggaaggg atattgccag
     9421 tacaaagtcg ttcaattttc tcgcaatatt tcttttttat acttttaagc tcgtcaaacg
     9481 gttgcttcat ataagaatat gcttccagtg ttttctcctg ttccaccata tgagcagata
     9541 ttctgtgttt aaccgattta acattagcca aacatgacct gtatctgtcg gtagcctctt
     9601 ttaatctttt gcggttttct tcctctatca gtcttaacca ctctttctcc cttctagcta
     9661 attcatattc tccaatagta aagccactca taaaccattc cctcctttat acaattatcc
     9721 ttattattat taatcgaata gattataaac atccccaccc acattgcgta tatcagcttt
     9781 tgctctttca gcttcaattc ttgtttcaaa tttaaatatc tgaggctctt cttttaattt
     9841 attgactgct tcattgtaac ttacatccag tattttggca acaaccaaag caataacatt
     9901 ttcacaacta ttgcaacgat agagtattat atcatagcta tcaggaatat cctcttcatc
     9961 tatcatctcc tgcatatcta attccattaa tttctctatt tcttttaatt cctctatttc
    10021 ctctatttct tctgttccct ctaatctctc aattgcttta tcaatcttcg ttattaactc
    10081 ttgaagttta atttcttcat taattttggg cttgttgaaa gttctgaata tatcttcatt
    10141 ctgcaacatc atcggtactc ttaaatcagt ttcaccaccc tcttgaacat atttagtaat
    10201 atcaaagtat ttcggattat tttctttttt atgttttaat tgcgccggtt ctttccactc
    10261 gctaaagtca aatatttcat catagaacgg taatttagtt atatacttga cacttccgtc
    10321 tatcattacc aatgcttgct ttacatccat accagcaagc tgctccggct taataaggct
    10381 tgttgtaact attcttgtac cgaaatctat ttccttatct ccgcattttt gtgataactc
    10441 ctctaaggtc ttccaatcgt tagtactaaa ggcaatgatg ataccgatat tggcaagtat
    10501 tgtagatgca ttactttttc catataaatc gtccaactga gataatgact gaagcacaat
    10561 atatatttta atatttctgc ttcgagatgt agccattaag tccggcaacc cggatatgga
    10621 acttcccaca cttgcaagct cctccagtat cagatgcata cgtatcggca atctgccttt
    10681 gtattcatct tgcgcaagac gcatatagtg tttcattaac tgactaatca acacaccgca
    10741 aagcctatca tagattattg attcatccgg caaaataata tatattgcaa ccggtttttc
    10801 accattcatt tctgcaatgt tcaagccatc agaactcaac atttcaataa gaccttcgct
    10861 tcttgcaaat attgccatac catctaagaa aacacatttt atacttcctc ttgtttcatt
    10921 aggcgcagcc atatacgaat tcaggttcat cgatgcagtt gtgtctttag gcaacaaatc
    10981 aataagatga cgcaaatagt ttgttgaccc taacctttgt tctcctaaaa cagcaagcat
    11041 atataccgac ttcatattta tttgatcagc aggagcaaaa ctgaaaagta aatagacaat
    11101 accgataaat aagctacgtg cactttgagc ccaaaacgga tcagcaacta cagaatccgc
    11161 aaataataaa aatgccaatt ctacaatcaa ttcataggct atttccatct tatgcttatc
    11221 accggtagaa tatagtttgt atgcaagttc taaaggattc caagtttcac tttctaaaat
    11281 atttctgaaa tcaattacat gaacaatata tttatctttc agaaaacaat aagttgcatt
    11341 gtatatctcc cctttaggat cggatacaat gtaggattca ccggcttgct gcactgatag
    11401 cactgtagag attgttccgc atctactttt acccttacca cttttaccga tatagagaac
    11461 atggctttct ccatcatata catatggctt accgttataa tatacaacag gaccacaggt
    11521 agaggattct ttatccaacc ggcttgtact cctttctatc tcgtactcat ttgaatagcg
    11581 gttcatcatc ttattttcct ccaaacatca agacaaaaca tcatgtctca tttttctcaa
    11641 ctgagaactg ccaactctcg atattaatga gctataatcc gttaagagta ttattctttt
    11701 tgataattct tttacatcat caattgtttt aagttccata tgtgcaatca gattaactaa
    11761 ttgttcatga aattgagctt tttgatcaat ttcaattccc ctcttttcta gcatcgactc
    11821 aactattaag gtcaattcag ttgcagtgta tacgcttttt tctattagaa cagtgtcttt
    11881 caatacatca cgaatcttat ctatcagttg actgatgctt ctagacggat tctcgggaac
    11941 ataaaaaatc aatgttgctg tttcactgac ttttgcaata ttatccaaaa ataattccac
    12001 ttgagtttca tgtatataat ctcctaatga atcaatacca aaaaacagaa ccccttcata
    12061 actatttgca taacaagctg ctaaactgat ataaccaaaa gactcttgaa gctgtttcaa
    12121 agtaccgtca aaatcaattt ccaaaaacaa gtctttgcta ctaaacttta taatactgtg
    12181 tttataaaac ttctcggtaa tatatcttgc aagagttgta cgaccgttac ccctatcaag
    12241 atggataata tagttgctcg gtcggactct tccgatacta tatgctttgc tgttcacgca
    12301 tatttcattt attgctttct ttatttcttc atggccgtag agcaatgtct ccctcatttt
    12361 tttcaccccc tctctatacc gatttaggaa accctatatt gtcaatcaat ccgtatgaca
    12421 atgcttcctt acttgtcata acataatctc tctccatatc tttgagcagt atatctaccg
    12481 gcttattaca ctttccggca aaaatagatg cgatatgttc tttggtcttt ttaatattat
    12541 cagcagccaa tattatatcc gttgcctgcc cttgaaccct acccaaaggt tgatgaatca
    12601 ttatttcggc attgggtaaa gcatagcgct tccctgattt cccgcaagcc aaaagaaaag
    12661 ctcccatact tgcggctctt ccgtacgcaa ctgttacaac atcacaatta accccgtatt
    12721 tgattgtgtc atatatggca agaccgtcct gtatagctcc tccggggctg tttatgaata
    12781 attctatatc atcacttgac ttgtttgata aatatccgag cattgacagc atcgctgtag
    12841 aggatgtact gtctatttca ccgaccaaat atattcttct tctcataaag tcaatattga
    12901 ctgaatcggt tatctgttca acaccgttaa tattcgttat tattgtgggt ataaatcttt
    12961 cattcatttt ttattccttt ccgtaaaata tgatgttatc cacccataat ttaccaagtg
    13021 attgtctaat tgaattttgc cacatttttt acacaaggtt cctaacaaaa tcctaacaaa
    13081 ataaggtatc ctgaatattg tgctactgtg ttcaatcatt gcaacaaatc tgttgttagc
    13141 tcagcttata tcgagtataa ttaaacacag ttgtatttcg tgaacattga tatgctgttc
    13201 caatgtatat acccacccag aataattagc tacttatgaa tttagcaact gctttcgaaa
    13261 atgtg
//
LOCUS       DAOSOM010000048        12160 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_5212_length_12160_cov_5.759603, whole genome shotgun sequence.
ACCESSION   DAOSOM010000048 DAOSOM010000000
VERSION     DAOSOM010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 12160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12160
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5212_length_12160_cov_5.759603"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1368)
                     /locus_tag="PLF14_08755"
     CDS             complement(<1..1368)
                     /locus_tag="PLF14_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOT71411.1"
                     /translation="MLEKNIIYDVLTAAVSKGADFAEIFLDDTVASNINYVDKQVEST
                     QSGRDFGIGIRVIKDLNCVYVYTNSFLKDDMIDTALKAAAAMESVKKDITFDLRKGQV
                     KNRNIIEIMPDTVTKQEKLEYLKRAYNAAITYDQCIKQASVTQMDKVQNILIANTEGL
                     LKRDRRVYTRMFISAVAEHNGEMQTGFTGPGSHRGFEFIKSLDIEQLAKEAAETAKKM
                     AYAGYCKAGTYPVVIDNGFGGVIFHEACGHSLEATSVAKGNSVFCGKSGQKIAADCVT
                     AIDDGTLPNEWGSLNIDDEGTVTKKNVLIKDGILKSYLIDNLNGKRMGMPSTGSGRRQ
                     NYRFAPTSRMTNTYIDAGKMKREDIISATDFGLYAKQMGGGSVNPGTGEFNFAVVEGY
                     MIRNGKIAEPVRGATLIGKGSEVLMNIDMVADNLEMAQGMCGSISGNIPTNVGQPAIR
                     VSKITV"
     gene            1588..2592
                     /locus_tag="PLF14_08760"
     CDS             1588..2592
                     /locus_tag="PLF14_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71412.1"
                     /translation="MRKILSILLVLIILSALPLTVSSAPAGVIHEQGVVYDGGRIYFI
                     YVNLKDGNTVKDSYYLALFKGDYFTVLPLSQLKADLNNQWMNHADRNYISPNTLSYAR
                     HGNNPQYRAVDGSFSVSQVVTYAANNLLNLPNAQILSTSYLDNRTVLSQNISNEDNHA
                     LYDSGTNTLTLNYTFNITEETVYITENGVTNEYLKKTYNSVQYKAICMLIELNAATVT
                     TPTATVRPTSTPTPTPTPTPTAKPTPTPTPTPTSTPTPTPVQTPSPSAQPSPAVSPAF
                     SASPEPTKTEKPTPTKVPDEIPERKNNKYEDIWTYVILQSLIILIIVIIVLSSRRKKR
                     "
     gene            complement(2589..4454)
                     /locus_tag="PLF14_08765"
     CDS             complement(2589..4454)
                     /locus_tag="PLF14_08765"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOT71413.1"
                     /translation="MPVISFNGVNKSYGGYDVLKDVSFELNADEKVALIGRNGTGKTT
                     IFNIIASGLEYDGGQVYVEKGLRIGYLRQLYTDLADKTAEAVIEEAFSHIKDMESELK
                     LLSKRLSADPHNADLLRSYGELSEKFEFHEGYSLNEKFNKVTKGLGIPDDVLNTRASK
                     LSGGELTMIMLAKVLLMQPDILLLDEPTNHLDCDASDWLEKYINEFKGTVLYISHDRY
                     FIDNTAMKVLYLQNGGIKEYRGNYSAYLVQKTEEEERHLKLYERQEREMARLRATALK
                     MRNYGTEIAIKRAKNLEKRISRMDIYDKPVTEKELKLTFLEEDKVAKEIFSVKNIEKS
                     FGSKTLFKDISFVMRSGDRIALIGPNGAGKTTLIKIITGQAEADSGIVKRPKSVKYAY
                     LEQKVSFPDETQTVLELVCNQLDLTLQSARNLLGKFLFYDDDVFKKVSSLSGGEKSRL
                     RLLLEMQQGVNLLVLDEPTNHLDIAAREEIEDALSMFGGSMIFISHDRHFINKFAERI
                     FEIENGSFNIYEGDYDYYLSKKQALMQQNKTVVPAVKKPFEGKKGKRKSDALKRTLEQ
                     DIKLTEYELDTVNAGIESNVTDYEILIELIKKRELLSSHLEELYQQWLDIEEDID"
     gene            complement(4479..5741)
                     /locus_tag="PLF14_08770"
     CDS             complement(4479..5741)
                     /locus_tag="PLF14_08770"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOT71414.1"
                     /translation="MDIKGKRIYDTMKKMNFIRLSTTEGEKSGAKVITDEIKAMGLEP
                     VFEDFKVPCYEIVNVKLEITEPKYMLVEAKGYGYSGNAAKDGITADFAYVEAAEDIDL
                     IDAEGKIVLVSNMGYEIYERLAKAKVAGFIAPSGGYFDDPKKTDLDERMLRKGHITYG
                     QIPGVSIRMKDAVKILKTNPKKAKLTLEQKEEEGDSGNIIVEVKGTKHPDEVIVYTAH
                     YDSVVFSKGMYDNASGSAILLELLRYFLKNPPLRTVRFIWCGSEERGLFGSKDYVKRH
                     EEQLKDIKLCINVDLAGPIIGRDTAIVLAEDKLCHMIEYMYKEVGHPMGLRQDIYSSD
                     CIPFADKGIPAVNFVRYGAPGAVSCHTRNDVLDVISPKTLENTARFVARFSERTVNAY
                     MFPVERKIPDDLVEKIDKYLRKKQLKED"
     gene            complement(5775..6791)
                     /locus_tag="PLF14_08775"
     CDS             complement(5775..6791)
                     /locus_tag="PLF14_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT71415.1"
                     /translation="MNMKKWLEDIKKSQVKKALPILSFPSIQLLNISVKELISSSDYQ
                     AKGMKAVADATDALAAVSLMDLSVEAECFGSHVVASDDEVPTVTGHVVETMQQAEDLQ
                     VPEVGSKRTGLYIEAIKKAKQLITDRPVLAGVIGPFSLAGRLLDVTEAMVLCYEDPDL
                     VHVVLEKCTEFIINYMEAYKKAGADGIMMAEPLGGLLSPSLADEFSSAYVKKIVDSVQ
                     TDDFAIIYHNCGDNVIQMIEGILDTGAAAFHFGNSIDMSVMMTKIPKDIIAMGNVDPA
                     AQIRNGTPRSVREATLAIMDKCSSYDNFIISSGCDIPPMAPWENINAFFDAVKEYNKI
                     HGRI"
     gene            7063..8169
                     /locus_tag="PLF14_08780"
     CDS             7063..8169
                     /locus_tag="PLF14_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HOT71416.1"
                     /translation="MTKLMENEKYLTEQIITYIGNKRSLLDFIGEAVEIVRTELNKEK
                     LDIADLFSGSGIVARYLKKYSNKIITNDLENYSYTLNKCYLSNEKDINIDKIVSYYNR
                     LKDRLEEKHISGFITKLYAPEDDGNIRKGERVFYTNKNARYIDTCRQTIATFPVDIQH
                     FFIAPLLTEASVKNNTGGVFKGFYKNRETGIGQFGGTAENALSRIKADIDIPFPVFSN
                     FNAEVINYQCDANALIDTLEEVDLLYLDPPYNQHPYGSNYFMLNLINDYIEPENISEV
                     SGIPEDWNRSGYNKKNKSLSILYDLCKKAKAKYILISFNSEGFITKEQMENMLKKTGT
                     VRTLKKKYNTYRASRNLNNRNTYVHEYLFLVRKS"
     gene            complement(8512..9693)
                     /locus_tag="PLF14_08785"
     CDS             complement(8512..9693)
                     /locus_tag="PLF14_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HOT71417.1"
                     /translation="MSEKIIHLNEELIKTELKDLVRSSVEETLNSLLDKEAEDLTNAQ
                     KYERTESRKGYRSGHYSRNLTTTSGEVTLKMPKLKGIPFETAIIERYRRRESSVEEAL
                     IEMYLAGVSVRRVEDITEALWGSKVSPSTISELNKKAYVNIEAWRNRPLTGGNYAYVY
                     ADGIYLKRNWGGEFENVSILVAIGVNEDGYREVIGAKEGMKEDKSSWLEFFTWLRERG
                     LKGVKLVIGDKSQGMIEAVVEVMPEAKYQRCLVHFYRNVFSCVPRSKMQYVVKMLKAI
                     HSQESKAAARKKALAVAEELKLMKLKEAADKVEKGIDETLTYMAFPYQHWTRIRTNNV
                     IERLNREIRRRTRVVGTFPDGNSALMLVCARLRHVAGTQWGAKKYLNMNHLDALNLMA
                     S"
     gene            complement(9965..10681)
                     /locus_tag="PLF14_08790"
     CDS             complement(9965..10681)
                     /locus_tag="PLF14_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HOT71418.1"
                     /translation="MHDPIANLLGSWSLDLNIWSILFRLILSIVFSAVVGMERSSKRH
                     AAGLRTFILLSTGATLAMIIDLVLIEEFGSTFALISTATIIGIAIISANTLLYSSKNQ
                     IKGLTTSVSMWTIGVIGLLFGAGYYTVAIIVGVVFVIVLSMLPAVERYLKNRSNHFEF
                     HLELVKASNLKDFITIIRKLGLRIDGIENNPAYVNSGLAVYSVSVSITKDELKQYKTH
                     KDIIEALNTLEYVYHIEEMV"
     gene            complement(10694..12130)
                     /locus_tag="PLF14_08795"
     CDS             complement(10694..12130)
                     /locus_tag="PLF14_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007157472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOT71419.1"
                     /translation="MNIYERLNITSTAAIVIISIGIMLFLGFLMTRITKKLKLPNVTA
                     YIVTGILIGPYVLNLIPREVVTGMDFLADIALAFIAFSTGEFFKLSVLKKNGPKVVVI
                     TIFESLLASILIFIVTYFVLGLTLAFSIVLAALAAATAPASTMMTIRQTGAKGDFVDT
                     LIQVVALDDIVGLVAYAIAISVAVASTTGEGVGFWAVVGPILVNIGVLLLGGLFGYFI
                     KFMLNKRSNDNRLIIALATLFVFCGICAMLDISPLLGCMSMGMVYINVTDDDNLFKQL
                     NYFSPPILLLFFVRSGVNFNLSALVNTSVFAGTLPLIVIGVVYFFVRIIGKYLGAFAG
                     SAVVKKDKKVRDYLGLALIPQAGVAIGLAALGARVLGGESGQALETIILASSVLYELI
                     GPACAKLSLYLSKSYSTKLEEISTVEEVTPTGKKKSPLEVLIERINEIQLEMPKIQEQ
                     RMKADEIAFTEAAEEYVAERKSGFINRK"
BASE COUNT         3479 a         2780 c         2043 g         3858 t
ORIGIN      
        1 tacggtgatt tttgaaactc ttattgcagg ctgccccaca ttggtgggta tattaccgct
       61 tatagacccg cacatgccct gagccatttc aagattatcc gctaccatat cgatattcat
      121 aagcacctcc gaccccttgc ctatcaatgt agctcctctt accggctcgg caatttttcc
      181 gtttcttatc atgtatcctt ccactacggc aaagttgaat tcgcccgttc cgggattgac
      241 cgatcctccg cccatctgtt ttgcataaag accgaagtcg gtggcactga ttatgtcttc
      301 tcttttcatt ttacccgcat caatatatgt gttggtcatt ctggatgtgg gtgcaaatcg
      361 gtaattctgt cttcgccccg aaccggttga aggcataccc attcttttac cgttcagatt
      421 atctatgaga taactcttta atattccgtc ttttatgagt acattcttct ttgtgaccgt
      481 accttcatca tcgatattga gactgcccca ttcattgggc aatgtgccgt catctattgc
      541 ggtaacgcag tcggcggcaa ttttctgccc cgacttcccg caaaataccg aattaccctt
      601 tgcaacagag gtggcttcca ggctgtgtcc gcatgcttca tggaatatta cacctccgaa
      661 accgttatct ataactacgg gataggttcc cgctttgcaa tagcccgcat atgccatctt
      721 ctttgccgtt tcagccgctt cctttgcaag ttgctctata tccagagatt ttataaactc
      781 aaaccctctg tgacttccgg gaccggtaaa tccggtctgc atttcgccgt tgtgttccgc
      841 aaccgcggag ataaacattc ttgtataaac tcttctgtcc cgtttaagca aaccttcggt
      901 attggcaata aggatgttct gcactttatc catctgcgtt accgaagcct gttttatgca
      961 ttgatcgtaa gtaatagcgg cattatatgc tcgttttaag tactccagct tttcctgctt
     1021 agttacggta tcgggcataa tctctatgat attacgattt ttgacctgtc cttttcgtaa
     1081 atcaaaggta atatcctttt taaccgattc catggccgct gcggctttaa gagccgtatc
     1141 aatcatatcg tcctttaaaa aactgtttgt ataaacatat acgcaattta agtctttaat
     1201 aacccttatg cctattccga aatctcttcc cgactgggtg gattccacct gtttgtccac
     1261 atagttaatg ttgcttgcca cagtgtcatc caaaaagatt tccgcaaaat ccgcaccttt
     1321 actcactgcg gcggtaagca catcataaat aatattcttt tccagcatat ccgtaccttc
     1381 ctttaatagt aacttagtta taattataca ccgtattact ctctttttat tattcacttt
     1441 aacatttgac ggttatatgg atgcataatt gccatatccg aagcagtagt tgccgacata
     1501 tacactgcca tatccgcagt cacagtgttc aactaccgat tgaatatatc ggctttatct
     1561 gctatcatat tgcaagggag aactttgttg agaaagatat tatcaatatt attagtactt
     1621 atcatactgt ccgcattacc gctcaccgtc agcagtgccc ctgcaggagt aatacatgag
     1681 cagggtgtcg tatatgacgg cggtcggata tattttatat atgtcaactt aaaagacggc
     1741 aataccgtta aggactcgta ttatcttgcc ctttttaaag gagattattt caccgtactt
     1801 ccgctgtctc aactgaaagc cgatctcaat aaccaatgga tgaatcatgc ggatagaaat
     1861 tacatttctc caaataccct ttcctatgcc cggcacggca acaatcccca atacagagcg
     1921 gttgacggaa gtttttccgt aagtcaggta gtcacatatg cggcaaataa tcttcttaat
     1981 ctgcctaacg cacagatatt gtccacatcc tatttggata accgtaccgt tctttcacaa
     2041 aacatttcca acgaagacaa ccatgccctg tatgacagcg gtaccaatac ccttacattg
     2101 aactacactt tcaatattac cgaagagacc gtttacatta ccgagaacgg cgttacaaac
     2161 gaatatctta aaaagacata taattcggta cagtacaagg caatatgcat gcttattgaa
     2221 ttgaatgcgg cgactgttac cacaccgaca gctacagtca gacctacttc tactccaact
     2281 ccaacgccaa cacctactcc tactgcaaag cctacaccta cacctactcc cacgcctact
     2341 tcaacaccta cacctacacc cgtacaaaca ccttcgccat cggcacaacc gtcacccgca
     2401 gtttcccctg cattttccgc ttctcccgaa ccgacaaaaa ccgagaagcc tacacccacg
     2461 aaagtccccg acgaaatacc cgagcgcaag aataacaaat atgaggacat atggacttat
     2521 gtaatactcc agtccctcat catcttaata attgtaatta ttgttctttc ctccaggagg
     2581 aaaaaacgtt aatcaatatc ctcttcgata tccaaccatt gttgatataa ttcttccaga
     2641 tgactgctta acagttcacg tttttttata agctcgataa gtatctcata gtcggttaca
     2701 ttgctctcaa tcccggcatt aacggtgtcc agctcatatt ctgtaagctt aatatcctgc
     2761 tccagcgttc ttttcagcgc atcgcttttt ctttttccct ttttgccttc gaaaggcttt
     2821 ttgactgcag gtacaaccgt tttattctgc tgcataagcg cctgtttttt ggacagatag
     2881 tagtcgtaat cgccttcata tatattaaaa gagccgttct ctatttcaaa tattctttcg
     2941 gcgaatttat tgatgaaatg cctgtcatgg gaaatgaata tcatgctgcc tccgaacatg
     3001 gataatgcat cttctatttc ctctcttgct gcaatatcca gatgattggt aggctcatcc
     3061 aataccagaa gattgactcc ctgctgcatt tccaaaagca gcctcagacg gctcttctcc
     3121 cctcccgaaa gactgcttac cttcttgaaa acatcgtcat cgtaaaaaag gaattttccc
     3181 agaagattgc gtgccgactg caaggtaagg tccagctgat tgcataccag ttccagtacc
     3241 gtttgcgtct catcgggaaa agatactttc tgctcaagat aagcgtattt aacgctttta
     3301 ggtcttttta caattccgct atccgcttcc gcctgcccgg taattatttt aatcagcgtg
     3361 gtttttcccg ctccgttggg accgataaga gcgattctgt ctccgcttct catgacaaac
     3421 gatatatctt tgaacagcgt tttgctgccg aaggattttt caatgttttt taccgagaaa
     3481 atctcttttg ccactttatc ctcttccaga aatgtaagct taagttcctt ttcggtaacc
     3541 ggcttgtcat atatatccat tcggcttatt cttttttcca gattcttggc tctttttatg
     3601 gcaatttccg ttccgtagtt tctcatctta agagcggtcg ctctcagtct tgccatttcc
     3661 ctttcctgtc tttcatacag tttgagatgt ctctcctctt cctccgtttt ctgaacaaga
     3721 tatgccgaat agttaccgcg gtattctttt ataccgccgt tctgaagata caataccttc
     3781 atggcggtat tgtctataaa atacctgtcg tgcgagatgt ataaaacggt gcctttaaat
     3841 tcatttatgt atttttccag ccagtcggaa gcatcacaat ccagatgatt ggtaggttcg
     3901 tccagaagca gtatatccgg ttgcatcagc aatacttttg ccagcattat cattgtcagc
     3961 tcgccgccgc tcaactttga agctcgggtg tttaaaacat catccggtat gcccagtccc
     4021 ttggttacct tgttgaattt ctcattcaga ctgtatccct catgaaactc gaacttttcg
     4081 ctaagctcgc cgtaagagcg caaaaggtcg gcattgtgag gatcggctga taaccgcttg
     4141 cttaatagtt taagttcact ttccatatcc tttatatgag aaaacgcctc ttcaataacc
     4201 gcttcggcag tcttatcggc aagatccgta tacagttgcc taagatagcc tatacgtaag
     4261 cctttttcca catacacctg tccgccgtca tattccagac cgcttgcgat tatattgaaa
     4321 atagtggttt ttccggtacc gttgcgacct ataagagcga ctttttcatc ggcattcaat
     4381 tcaaaactga catcttttaa gacgtcatat ccgccgtaac tcttattcac accgttaaaa
     4441 gatataacag gcattgttac ctctacggta agtatatatt aatcttcctt caactgcttc
     4501 ttacgaagat atttatctat cttttccacc aaatcatccg gtatttttct ttccacagga
     4561 aacatgtagg cattgacggt tctttcggag aaccttgcta caaaacgggc ggtattctcc
     4621 agagttttcg gtgaaataac atccagtaca tcgtttcttg tatgacagga aacggcaccg
     4681 ggtgctccat atcgcacaaa attgacagcg ggtatacctt tatctgcaaa aggaatgcaa
     4741 tcggaagaat atatgtcctg tcttaagccc atgggatgtc ccacttcctt atacatgtac
     4801 tctatcatgt gacacaattt gtcctccgcc agcacaatgg ccgtatccct ccctattatc
     4861 gggccggcaa gatccacatt tatgcacaac tttatatcct tcaattgttc ctcatgtcgt
     4921 tttacataat ccttacttcc gaaaagacct ctctcttccg aaccgcacca gataaatctg
     4981 accgttctca gaggaggatt tttaaggaaa tatcttaaca gttctaaaag tattgcagaa
     5041 cccgatgcgt tatcgtacat acctttactg aagacaaccg aatcgtaatg agccgtatag
     5101 actattactt catcgggatg ttttgtgcct tttacctcca caattatatt acccgaatcg
     5161 ccttcttctt ccttctgctc taaagtaagt tttgcttttt tcggattggt ctttaatatc
     5221 tttacggcat ctttcattct aatggatact cccggtatct gaccgtaagt aatatggcct
     5281 tttcttaaca tccgttcatc cagatcggtc ttcttgggat catcgaaata gcctccgctc
     5341 ggtgcaataa atcccgctac ttttgccttg gcaaggcgtt catatatctc atagcccata
     5401 ttggagacca atacaatctt accttcggca tctattaagt ctatgtcttc ggccgcttcc
     5461 acataggcaa aatccgccgt gataccgtct tttgcggcgt taccgctgta tccgtaacct
     5521 tttgcttcca caagcatata tttaggttcg gtaatctcca gtttcacatt tactatctca
     5581 tagcaaggaa ctttaaaatc ctcaaaaacc ggctccaaac ccattgcctt tatctcatcg
     5641 gttatgacct ttgctccgct tttctcaccc tcggtggtgg aaagccggat aaagttcatt
     5701 ttcttcatcg tgtcatatat tcttttacct tttatatcca tacctttttc cttaactgct
     5761 ttaccttttc ttctttaaat tcttccgtgt attttattgt attcctttac cgcatcaaag
     5821 aacgcattaa tattctccca cggagccatc ggaggtatat cacatccgct ggatattatg
     5881 aagttgtcat atgatgaaca tttatccatg atagcgagtg tcgcctctct gaccgatcgg
     5941 ggtgtgccgt ttcttatctg agctgccggg tcgacattac ccattgcaat tatatctttt
     6001 ggtattttgg tcatcataac cgacatatca atggagtttc cgaaatggaa tgccgctgca
     6061 ccggtatcca gtataccttc tatcatctga attacattat ctccgcaatt gtggtagatg
     6121 attgcaaaat catcggtttg aacactatcg acaatcttct ttacataagc cgatgagaac
     6181 tcatccgcca atgacggtga cagaagacct cctaaaggtt ccgccatcat tattccgtcc
     6241 gctcccgctt ttttgtatgc ctccatgtag ttgataataa actcggtaca tttttccagc
     6301 acaacatgca ccaggtccgg atcctcataa caaagtacca tcgcttcggt tacatccaaa
     6361 agccttcccg ccaaggagaa aggtcctatt acaccggcaa gtaccggtct gtcggttatc
     6421 aactgttttg cttttttaat tgcttctatg tataaaccgg tccttttact tccgacttcg
     6481 ggaacctgta aatcttcagc ctgctgcatg gtttccacta catgaccggt tacggtaggc
     6541 acttcgtcat ccgatgcaac cacatgtgag ccgaagcatt cggcttccac cgaaaggtcc
     6601 ataaggctta cagccgcaag cgcatcggtc gcatccgcta cggccttcat gccttttgcc
     6661 tgataatcgg aactggatat cagttccttt acacttatgt tcagtagctg tatcgagggg
     6721 aaagataata taggcaaagc ctttttcacc tgacttttct taatatcttc cagccatttc
     6781 ttcatattca tttaaaacac ctccacatga catatgataa attgtatttc gcaccgtttc
     6841 ttattctacc ataaaaaaag acctgtgccg cattatatgt gcaaatatct tatcttttct
     6901 tttttttctt ttttatcacg aaaccaaact ttgtactctg atattttctt atttaactct
     6961 gcttgtaaaa ttaacggaat attgtgtaaa aaaatgatat gatttaatag tgttaaataa
     7021 caaagcgaca attcttccga tatggtgaca aagaaaaaat atatgacaaa actgatggaa
     7081 aacgaaaaat acttaaccga acagattata acgtatatag gtaacaaacg ttccctgctg
     7141 gatttcatcg gagaagcggt cgagatagta agaacggaac tgaacaaaga aaaactggat
     7201 atagcggacc tgttctccgg ttcgggaatt gttgcgcgat acttaaaaaa atattctaat
     7261 aaaattatta ccaatgattt ggagaattat tcatatacgt tgaataagtg ttatttaagc
     7321 aatgaaaaag atattaatat tgataaaata gtaagttatt acaataggct aaaagataga
     7381 cttgaagaaa agcatataag cgggtttatt acaaaactgt atgctcccga agatgacggc
     7441 aatataagaa agggagaaag agttttctat accaataaaa acgcaagata tatcgatacc
     7501 tgcagacaga cgatagcaac ttttcctgtc gatattcaac acttttttat agcaccgtta
     7561 ctgaccgagg cctcggtaaa aaacaatacc ggaggagttt tcaaaggttt ctataaaaac
     7621 agggaaacag gcatcggaca gttcggaggt actgcggaaa atgcattgtc aagaataaaa
     7681 gcggatatag acattccctt ccctgttttc agcaatttta atgcggaagt tataaactac
     7741 cagtgtgatg ccaatgctct gattgataca ttggaagaag tggacttatt gtatttggac
     7801 ccgccgtata atcagcatcc ttacggttcc aattacttca tgcttaactt gattaatgac
     7861 tatatagagc ctgagaatat cagtgaggtt tccggtattc ccgaagactg gaacaggtcg
     7921 gggtataaca aaaagaataa atctttaagt atactgtatg acctttgcaa aaaagcaaaa
     7981 gcaaaataca tactcatttc ctttaactcc gaaggcttta taaccaaaga acaaatggaa
     8041 aatatgttga aaaaaacagg aaccgtccga acactgaaaa aaaagtacaa tacctacaga
     8101 gcttccagaa acttgaataa ccgtaacacc tatgtgcatg aatacctgtt tttggtaagg
     8161 aagtcataag aacaccgttt atatattttc tgatcgctcc ttagtatcgg ataacctgtt
     8221 tcttatcgct tgattgcaga tttatatgga catttgccct tataaacatt aaaaagaaga
     8281 taaatggctt tgattaccta ttttgataca attacgcacc cctagcagaa atcacgcacc
     8341 cttgaaggta aatcgtgcac ccctgactag aactacgcac ccctaactta ttgaccatta
     8401 cttgttaact tcgataaaag ttctgtatag ttaatcgctt tctattcctt tgtcttattg
     8461 gtactgtcaa gaaaatttcg caaattagtt tgcagcctgt ttgaaattgc tttaacttgc
     8521 cattaaatta agggcgtcca gatgattcat gttaagatat ttcttagcac cccattgtgt
     8581 gcctgcgaca tgtcgcaggc gcgcgcacac gagcatcagc gctgagttgc cgtcggggaa
     8641 agttcctacc acgcgggttc ttcttcgtat ctcacggttt agcctctcaa tgacgttgtt
     8701 ggttctgata cgcgtccaat gctgatacgg aaaagccata taggtaagag tttcatctat
     8761 tcctttttca accttgtcgg ccgcttcttt aagcttcatt aatttcagtt cttcggcaac
     8821 ggccaaagct ttctttcggg cggctgcttt gctctcttga gaatgaatcg cttttagcat
     8881 tttcactaca tactgcattt tagaccgcgg tacacacgaa aatacattgc ggtaaaaatg
     8941 taccaggcaa cgctggtatt tagcctccgg catgacctct acaaccgctt ctatcatacc
     9001 ttggctctta tccccaataa caagttttac gccttttaag cctcgttctc taagccatgt
     9061 gaaaaattca agccaactgc tcttatcctc tttcatgcct tcttttgcgc cgattacctc
     9121 gcgataaccg tcctcattta cacctatcgc cactaatata ctgacatttt caaattcccc
     9181 gccccaattt cgctttaaat agattccgtc ggcgtacaca taggcataat tgccgcctgt
     9241 aagcggcctg ttccgccagg cttctatgtt gacataggct ttcttgttta attcgcttat
     9301 ggtggaaggg gagaccttgc ttccccataa tgcctcggtg atatcttcca cccgacgaac
     9361 cgatactccg gcaaggtaca tctctattaa agcttcctca accgagcttt cacgccggcg
     9421 gtacctttct atgattgccg tttcaaaagg tatgcctttg agcttaggca tttttaatgt
     9481 tacctcacct gatgttgtgg ttaaatttct gctatagtgt cctgaacggt aacccttcct
     9541 tgattctgtg cgctcatact tttgtgcgtt agttaaatcc tccgcttcct tgtctaaaag
     9601 ggaattaagg gtttcctcta cgctgctgcg aaccaaatcc tttaattctg tcttgattaa
     9661 ttcctcgttt aagtgtataa tcttttcgga cattcttgct tcctccttag gtaaaatttg
     9721 ttgtgataaa tttattctac caaacaggtt gcaagtctgt ctattttttt aatttgcgaa
     9781 atttattgta ccttatctga acatcaaacg atattgatat tttagcactt tattttataa
     9841 tgtcgattat tttgatgttc aaagcattag taaacgctta cacaatttta atataaaaaa
     9901 agatgtccct tactatttat agtaaagaac atcttgtaat aattatctaa tttagttata
     9961 tatattaaac catttcttca atatgatata catattctaa ggtatttaaa gcttcaataa
    10021 tatctttatg agtcttgtat tgttttaact cgtcttttgt aattgaaacc gaaactgaat
    10081 aaacagctaa tccagaattt acatatgctg ggttattttc aataccatca attcttaaac
    10141 caagttttct aataatagta ataaaatctt ttagatttga tgctttgact aattccaaat
    10201 ggaactcaaa atgatttgaa cgattcttta aataacgttc aacagcaggt aacatggata
    10261 gcacaatgac aaacaccaca ccgacaataa tagcaacagt gtaatatcct gcaccaaaca
    10321 gtaatccaat cactccaatt gtccacattg aaactgaagt tgttaaccct ttgatttggt
    10381 tcttagaact ataaagtaga gtattggcac taataattgc aataccaata atggttgcag
    10441 ttgaaattag agcgaaagtt gaaccaaatt cttcaatcaa tactaaatcg ataatcattg
    10501 cgagtgttgc gccagtagaa agtaagataa atgtacgaag acctgcagca tgtctcttac
    10561 ttgaacgttc catacctaca accgcagaga atactattga taaaattaat ctaaataaaa
    10621 tactccaaat atttaaatct aaagaccaac tacctaataa atttgctatt ggatcatgca
    10681 tcctcattca cctctatttt ctattaatga atcctgattt acgttctgca acatattctt
    10741 cagcagcttc tgtaaatgca atttcatctg ccttcattct ttgttcttgt atctttggca
    10801 tttctaactg aatctcattg attctttcaa ttaaaacttc aagtggagat tttttctttc
    10861 ctgttggagt aacttcttcg acagttgaaa tttcttctaa cttagttgaa taagatttag
    10921 ataggtataa tgatagttta gcacaagctg gtcctataag ttcataaagg actgatgagg
    10981 caagaataat cgtctctaat gcttggcctg attcaccacc taagactctt gcaccaagtg
    11041 ctgctaaccc aatcgcaacc cccgcttgag ggattaaagc taatcctaga tagtccctaa
    11101 cttttttatc ctttttaaca acagcactac cagcaaatgc acctaagtac ttacctataa
    11161 ttcttacaaa gaagtaaacg acaccaataa caattaatgg taatgttcct gcaaatactg
    11221 aagtgtttac taaggcactt aaattaaaat tcacacctga tctcacaaag aataacaata
    11281 aaattggagg gctaaagtag ttaagttgtt tgaatagatt atcatcatct gtcacattaa
    11341 tataaaccat acccattgac atacaaccaa gtaatggtga aatatctaac atagcacaga
    11401 tgccacagaa tacaaataat gttgctaagg caataattaa acggttatca tttgagcgtt
    11461 tattaagcat aaactttatg aagtatccaa ataaaccacc taataacaag actcctatat
    11521 taaccaatat aggcccaaca acagcccaga atcctacacc ttcacctgta gtagatgcaa
    11581 cagcaacaga aatagctatc gcgtatgcaa caagtcctac aatgtcatct aatgcaacca
    11641 cttgaattaa agtatcaacg aaatctcctt tagcaccagt ctgacggata gtcatcatgg
    11701 tagatgctgg agctgttgct gcagctaaag ctgctaacac aatagaaaac gctaaagtta
    11761 atcctaaaac aaagtaagtt acaataaaga taagaataga tgctaataat gattcaaaaa
    11821 ttgtaataac tacgacttta ggaccgttct tttttaatac tgataattta aagaattcac
    11881 ctgttgagaa tgcaataaat gctaacgcaa tatcagctaa gaagtccatt ccagttacaa
    11941 cttctctagg tattaaattt aacacgtatg gtcctattaa aataccggta acaatatagg
    12001 ctgtaacatt tggtaatttt aattttttag taatacgagt cattaagaaa cctaagaaca
    12061 acataatacc aattgaaata ataacaattg ctgcagttga tgtgatattc aatcgttcat
    12121 aaatgttcat aaaaatcctt cttttctaag atttaatatt
//
LOCUS       DAOSOM010000049        11944 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_5310_length_11944_cov_6.475482, whole genome shotgun sequence.
ACCESSION   DAOSOM010000049 DAOSOM010000000
VERSION     DAOSOM010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 11944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11944
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5310_length_11944_cov_6.475482"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..1375)
                     /locus_tag="PLF14_08800"
     CDS             complement(89..1375)
                     /locus_tag="PLF14_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002817934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOT71420.1"
                     /translation="MPVNEVINISHKILLIPNNKAITHFRKAFGCSRLAYNWGLNKWQ
                     EYYKQGIKKTHLELKKEFNSIKKEEFPFVYEVSKYATQQPFLHLNAAFKKFFRDLEKG
                     KISYPKFKKKSKNSGSYYIGGDHVVIKDEKYLKIPNLGLVKMREKLRFDGKINSVTIS
                     QNGDKFYASFSMQITKQTYADTHKKPKRNNIALGIDVGLNSFLSLSNGLEVKAPKPLK
                     KLERTLIRRSRQLNRKQHAKTKQEALDGVESSKNFIKASRKLNKLYSRITNIRKDYLH
                     KLTSSLVANADYFCLEDLNVQGMMQNHRLAKSLSDVSFYEFKRQLEYKAAYNDKQIIQ
                     VDRFYPSSKMCSKCGNIKTDLSLKDRVYYCQTCGTIIDRDYNASLNMLSHLQKAIGQV
                     RPEFTLADLTALQLDLAINQIVTSKAEAGIQQKYCA"
     gene            complement(1761..2141)
                     /gene="rplL"
                     /locus_tag="PLF14_08805"
     CDS             complement(1761..2141)
                     /gene="rplL"
                     /locus_tag="PLF14_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOT71421.1"
                     /translation="MSEKITKLIEEIKTLTVLELADMVKALEEEFGVVAAAPVAAAAA
                     AGPAAAVEEVEEQTEFNVLLKEIGPEKIKVIKVCREATGLGLKEAKDLVDNAPSTIKE
                     AISKQAAEELVAKFKEVGATAEIK"
     gene            complement(2172..2681)
                     /gene="rplJ"
                     /locus_tag="PLF14_08810"
     CDS             complement(2172..2681)
                     /gene="rplJ"
                     /locus_tag="PLF14_08810"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOT71422.1"
                     /translation="MASEKILEVKKQQQAELREKLLSMGSVILVDYRGLTVAQDTAMR
                     SDMKKADIEYKVIKNRLLIKAMEGTKYEGLIPHLEGPTAVAMSEDEIMAAKMAAKYAN
                     MKDYSKFQLKAGAIGDQIVDVDGIQAYAKIPAKNDLLAMLLGGMKAPITSLALVLKAI
                     MEKKEETAA"
     misc_feature    complement(2688..2823)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(2875..3573)
                     /gene="rplA"
                     /locus_tag="PLF14_08815"
     CDS             complement(2875..3573)
                     /gene="rplA"
                     /locus_tag="PLF14_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOT71423.1"
                     /translation="MKRSKNYRASLELYDKTASYEPSEAINLVKSTAKAKFDETIELH
                     IKLGVDSRHADQQVRGAVVLPHGTGKNVRVLVFAKGPNADAAEKAGADYVGAEELADK
                     IKNENWFDFDVAIASPDMMGVVGKIGRILGPKGLMPNPKAGTVTTDVAKAVTEAKAGK
                     IEYRLDKTNIIHCPIGKASFEPEKLMDNFRTLMNAVIKAKPAAAKGQYLRSITLASTM
                     GPGIKVNVQKVLDL"
     gene            complement(3586..4011)
                     /gene="rplK"
                     /locus_tag="PLF14_08820"
     CDS             complement(3586..4011)
                     /gene="rplK"
                     /locus_tag="PLF14_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOT71424.1"
                     /translation="MAKKIIGYVKLQLPAGKATPAPPVGPALGQHGVNIMGFCKEFNE
                     RTKNDIGMIIPIVMTVYADRTFSFVTKTPPAAVLLKKACNVESGSAVPNKTKVATVSK
                     ADLRKIAEQKMTDLNAGSVEAAMSMVAGTARSMGIKVEE"
     gene            complement(4044..4607)
                     /gene="nusG"
                     /locus_tag="PLF14_08825"
     CDS             complement(4044..4607)
                     /gene="nusG"
                     /locus_tag="PLF14_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOT71425.1"
                     /translation="MEENLNTEITEKREEQDLARWYVVHTYSGYENKVKADLEKIVEN
                     REMQEYIQEIVVPLEEVIEEKNGKRKITFKKIFPGYVIVKMIMNDQSWYVVRNTRGVT
                     GFVGPSARSVPLTAEEVIQMRLEGMETVIDFNVGDNIRVIEGPFENFVGIIKEVDKDK
                     RKVKVHISIFGRETPVELDYDQIQIIL"
     gene            complement(4625..4837)
                     /gene="secE"
                     /locus_tag="PLF14_08830"
     CDS             complement(4625..4837)
                     /gene="secE"
                     /locus_tag="PLF14_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002842917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOT71426.1"
                     /translation="MAGKEKKSFGSKFTGFFKGIAQELKKVTWPTGKQLFRSTVTVLV
                     VCAIVAIIISLFDWGLSSLIKLLVKG"
     gene            complement(4851..5000)
                     /gene="rpmG"
                     /locus_tag="PLF14_08835"
     CDS             complement(4851..5000)
                     /gene="rpmG"
                     /locus_tag="PLF14_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOT71427.1"
                     /translation="MRDKIILACTECKQRNYSTKKNKKNTPDRLEFSKYCKFCRKHTP
                     HKETK"
     gene            complement(5121..5942)
                     /locus_tag="PLF14_08840"
     CDS             complement(5121..5942)
                     /locus_tag="PLF14_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71428.1"
                     /translation="MDDLRKKYLELLELNEDCTETEINKKYDNLSLRAKTDKSIDIDE
                     VIKAYDYLMNRNLEEPEPVKPIVKKFRKAWFKYIGLAAILLIILGTVLSIVIPYITNK
                     DPDLVISYIGPYTPKNNKDLENYLYDRMPKVKKILMETIYATAAGNYVRFKEDSYDAS
                     SVEKLSLLLISGGLELIVCDDAVYRYILSYNYLLELDEIIEEYGIKIDKKNIVYGIKP
                     STGERVIYGINIQDYPILSEHVYINEEFPMILCVSNEAVNIENVKQAVRILLTEE"
     gene            complement(6118..7371)
                     /locus_tag="PLF14_08845"
     CDS             complement(6118..7371)
                     /locus_tag="PLF14_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009279554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT71429.1"
                     /translation="MNQIFDELLYFREKYLSKIRGFYHEGEIIKVLTGVRRCGKSSIL
                     KMIVRELLSQGVKEENILFFNLDRRPYTTTNTAEQLDCLIEKNNKASGMKYLFIDEIQ
                     NVKGFETVINSWREEGDYSIFITGSNSYLLSGELVTKLTGRYLEFNILPLTLEEFVGM
                     KMFFGKEVPADKQDILSEYIYEGGFPYAVRLDSLKDKRAYVTGLIEEIYQKDIRSRIQ
                     IRNRTVFDAVLKYIVNNYGTTTSIENIVKDFRKNNVSVKRETIARYIKALENAKIISE
                     CRRFDMKSRKSLRGEKKYYLSDLSFFFAMNTDNRINFGPVLENIVYNYAFSMGYSISI
                     GKIGKLECDFILRDTELNYAYVQVAYTILESKATEDREYRSLEAITGDNYPRYVMTTD
                     RLIQYRNGINHVNLIDFILENKSFE"
     gene            complement(7518..9008)
                     /gene="lysS"
                     /locus_tag="PLF14_08850"
     CDS             complement(7518..9008)
                     /gene="lysS"
                     /locus_tag="PLF14_08850"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOT71430.1"
                     /translation="MDKIVENTETDLNEILRIRREKLEALKQEGRNPFDITRFDREYT
                     AKEILDNFDSLQNKEVSLAGRIMAKRIMGKASFCHIQDGLDRIQLYVRIDELGEDTYS
                     EFRKYDIGDIVGVTGFPFITRMGEKSIRVKSIVLLTKSLQVLPEKFHGLKDMDLRYRQ
                     RYVDLIVNPDVKSVFITRANIMKAIREFMHSLGYLEVETPVLHNQATNANARPFATYH
                     NTLNIDMYLRIELELHLKRLIVGGLDKIFEIGKVFRNEGMDTRHNPEFTLMELYEAYA
                     DMYTMMERAEQIFCYIADNVSGTRKIDYQGQIINVEAPWRRLTMIEAVKEYSGVDFDS
                     FTLDDAAARAAAKKHNVRIEKHWNWGMILNAFFEEYAEKNLIQPTFIYQYPIEISPLA
                     KRNKEKPHLTERFEFFVLGNEMGNAFSELNDPVDQRERLIAQAKSKFGEYDFTIDDDF
                     INALEVGMPPTGGMGLGLERMIMLFTNSTSIRDVMFFPTMKPESNG"
     gene            complement(9100..9576)
                     /gene="greA"
                     /locus_tag="PLF14_08855"
     CDS             complement(9100..9576)
                     /gene="greA"
                     /locus_tag="PLF14_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOT71431.1"
                     /translation="MAEKKVLMTYEGIKELEDELATLKGKTRMEVSDRLKSAISFGDI
                     AENSQYDEAKNEQAQLEARIMYLENLLMNAEVIDSENIDTTNVTIGCRVKILDLEFKE
                     EEIYQIVGPTEADPFNMRISYESPIGKAILGKEAGETVEFESPAGPVKIKILEITE"
     gene            9813..11399
                     /locus_tag="PLF14_08860"
     CDS             9813..11399
                     /locus_tag="PLF14_08860"
                     /inference="COORDINATES: protein motif:HMM:NF024602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HOT71432.1"
                     /translation="MKQLFKELVFKSEAVCTDFFNDVTFDVKFTRPDGSNLLIPGFWA
                     GGNTFKARYDISVPGKHGYVTVSNDKSLNGISGQLVIEKEKTDNPYYLHGPVCRKGQN
                     MYLSHSDGTPFYWFGDTWLMGLTKRLPFPDDFCTLLNDRKEKGFNVIQIIAGLYPDMT
                     MFDPRGENEAGFPWKEDLSAINPEYFDRADERIEKLVENGIMPCIVGTWGFYMFFAGK
                     EILKKHWRYIIARWGAMPVCWCVAGEADMPFYNHNVPYEEHIKKAKKDWNDMTVFIKQ
                     TDPYARLITIHPFPASDGYDQIEDGSLLDLNMLQSGHGGFSALPEQMKKVRKAVERKV
                     LPAINAEVCFEGICGASYADTQRYVYLSDFLLGAAGHTYGANGIWQVNSEEMPYGASP
                     HGAHWGNISWREAMNFEGAKQISLCMKYLTSFEWWKFRYHPEWLETPCSLNACDGHFA
                     AGIPKKVRVIFKPFFGGSFFGDINILKIEKGINYKAYRFNPITGKETYLADVTPDNEG
                     KWKFPRADAFQDWLYVLKAE"
     gene            complement(11434..11934)
                     /locus_tag="PLF14_08865"
     CDS             complement(11434..11934)
                     /locus_tag="PLF14_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005962187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOT71433.1"
                     /translation="MTVYTGTIEMQSKDHMPDFHNITDEVKKIVEQSGVKNGICVVYS
                     HHTTCSVMTQECSHDTTYFGREHLQQDLINIMEKIVPTCRHEGQYLHPGPKHIEFALT
                     WPGEEPKGSLNTDAHLRSCFFGRSETIVITDGKLSLGDFGFIYFIDWDQVRERKRVAE
                     VQIIGE"
BASE COUNT         3171 a         2666 c         2094 g         4013 t
ORIGIN      
        1 cagaccttcg gtataaataa tcgagtagag cagaggcaaa atgaaaccgt tcctacaaac
       61 ctataaaata ccgcatatag gaaaaaacct atgcacaata tttctgttgt attcctgctt
      121 ctgccttgct ggttacaatc tgatttatcg ctaaatccaa ttgcagagcc gtcaagtccg
      181 caagcgtaaa ttcgggtcga acttgcccta ttgctttttg taaatgtgaa agcatattca
      241 aacttgcatt gtaatctcta tcaatgatgg taccgcaagt ttgacagtaa tacaccctgt
      301 cttttaaaga taggtctgtt tttatgtttc cgcactttga gcacattttt gaactcggat
      361 aaaacctgtc tacttgaata atctgtttat cattgtaggc agctttgtat tcaagttgtc
      421 tcttaaactc ataaaagctc acatcactta aagattttgc caaacgatga ttttgcatca
      481 tgccttgaac attcaaatct tctaaacaga agtaatccgc atttgcaaca agtgaagaag
      541 taagcttatg cagataatcc tttctgatgt ttgtaatcct gctatacaac ttattcagtt
      601 tacggcttgc ttttatgaaa ttcttagagc tttcaacacc gtccagtgct tcctgcttgg
      661 ttttcgcatg ttgctttctg ttcagttgtc tgctgcgtct gataagagtg cgttccagtt
      721 tcttaagggg tttcggtgct ttaacttcca atccgtttga taagcttaaa aagctgttta
      781 aacctacatc aattccaagt gctatattat ttcgtttggg tttcttatgt gtgtctgcat
      841 aagtctgctt tgttatctgc atactgaaac ttgcataaaa tttatctccg ttttggctta
      901 tggtaacact gtttattttc ccatcaaacc tcagtttttc ccgcatcttt acaagaccca
      961 aattgggtat ctttaaatac ttttcatcct ttattacaac atgatcgccg cctatgtagt
     1021 atgagccgga attcttgctt ttctttttga acttgggata tgagatttta cctttttcta
     1081 aatctctaaa gaacttctta aatgcggcgt ttaagtgaag aaagggctgc tgagtagcgt
     1141 atttgctcac ttcatagaca aagggaaatt cttctttttt gatagagtta aactctttct
     1201 ttaattcaag atgtgtcttc ttaatgcctt gtttgtaata ttcctgccat ttgttaagtc
     1261 cccaattgta tgctaagcgg gagcaaccga aagcctttct gaaatgtgtg attgccttgt
     1321 tattaggtat caataatatt ttgtggctta tgttaataac ttcatttacc ggcattgtct
     1381 tctaccgcct tttgcactaa gaatctttgt taattaacct atttgcttat attaaaattt
     1441 tagtagatta cacacagaaa gtccatgata acatatcgca aattatgtaa tatagtatat
     1501 gcggttgata tttttgagcg attcaaactt gtatccgtgt tcgccaccac gaatatactc
     1561 atccggtttg agcagacttt ttattctgat tttttagcct ttgcggccta tatataatag
     1621 tcatgctctt attttaacag ctactttgat tgtcaacata tagaatacat tatatatatt
     1681 aatatgttct tatatattta tactacaatt gaaaacccct tttaaagggg tttccatgct
     1741 tggatttttt gtttttcaaa ttacttaatt tccgctgtag cgccgacttc tttgaatttg
     1801 gcaaccagtt cttcggcagc ctgtttggat atagcttctt tgattgtact gggagcatta
     1861 tccacgaggt cttttgcttc tttaagtccc aaacctgtag cttctctgca caccttgata
     1921 accttaatct tttccggccc tatctcctta agcaatacgt taaactcggt ctgctcttcc
     1981 acttcttcaa cagcggctgc gggacctgct gctgcggctg ctgctacggg agctgcagcg
     2041 acaacgccga actcttcttc cagagcttta accatatcgg caagctccag taccgttaat
     2101 gttttgattt cttctattaa ttttgttatc ttttcactca ttgtaattat cctccgatta
     2161 tttaatttcg cttatgcagc ggtttcttct ttcttttcca tgattgcctt aagaaccagt
     2221 gccaatgaag taataggcgc tttcataccg cccaacaaca tagcaagcaa atcattcttg
     2281 gcaggaatct tggcgtatgc ctgaattccg tcaacatcta ctatctgatc accaatagca
     2341 ccggctttga gctgaaattt ggaataatct ttcatgttgg catacttagc cgccattttt
     2401 gcagccataa tctcatcttc gctcatagca actgcagtgg gcccttccaa atgaggtatc
     2461 aatccctcat atttggtgcc ttccatagct ttaataagaa gcctgttttt gatgacctta
     2521 tactcgatat cagctttttt catgtcagaa cgcattgcgg tatcctgagc aaccgtaagt
     2581 cctctgtaat caaccagtat gactgatccc atggacaaca gcttttctct caattccgcc
     2641 tgttgctgtt tcttgacttc caatatcttt tcactagcca ttatttacct ccttataaaa
     2701 aatcctcgca tactgcacgc atacgaggat tggataataa atcaatcttg tatcttcctc
     2761 ggcgggattt acgcttttct tacgaattgc acctgctgtc tgcggcagat ttactttatt
     2821 tacttttcat tacggatata atccgcaact tttccaataa taagccaaaa gctcttacaa
     2881 gtcaagtact ttctgtacat ttaccttgat tccggggccc atggtcgaag ccaatgtaat
     2941 actcctcaga tattgtcctt ttgctgcggc cggttttgcc ttaataaccg cattcataag
     3001 tgttctgaag ttgtccatca acttttccgg ttcaaacgat gcttttccga tcggacagtg
     3061 gataatgttt gttttgtcca gtctgtactc aatcttacct gctttggctt cggttacggc
     3121 ttttgccacg tcggttgtta cggtacctgc tttggggtta ggcattaatc ctttgggacc
     3181 caaaatacgt cctatctttc ctaccacacc catcatatcg ggactggcta tagcaacatc
     3241 aaaatcaaac cagttctcgt tcttaatctt atccgcaagt tcttccgctc ccacatagtc
     3301 ggctccggct ttctcagctg cgtctgcatt gggtcctttt gcaaatacca atactctgac
     3361 gtttttaccg gttccgtgcg gtaatacaac agcacctctg acctgctgat cggcatgtct
     3421 cgaatccaca cccagtttga tatgcaattc aattgtttca tcaaacttag ctttagctgt
     3481 ggatttaaca aggtttatgg cttccgaagg ctcatatgag gcggttttat cgtataattc
     3541 caaacttgct ctgtagttct tgcttctttt catttacact tcctcctatt cctctacctt
     3601 gatgcccata ctgcgtgcag taccggcaac catgctcata gcagcttcaa cgcttcccgc
     3661 attgaggtct gtcatcttct gttccgcaat cttccttaag tcagctttgg aaactgtcgc
     3721 aactttggtc ttgttaggga ccgcagagcc gctttccaca ttgcatgcct tcttaagcag
     3781 taccgctgcg ggcggagtct tcgtaacgaa agaaaaggtt ctgtctgcat agaccgtcat
     3841 cactattgga atgatcattc ctatgtcatt ttttgttctt tcattgaatt ctttacagaa
     3901 tcccataatg tttacaccgt gctgtcctag tgctggtcca accggcggtg ctggcgttgc
     3961 tttacctgca ggcaattgca attttacata tccgataatc ttttttgcca ttcaatccac
     4021 ctcccaaatg tattttttca agcttaaaga ataatctgta tctgatcata gtccaattca
     4081 acaggtgttt ccctgccgaa tattgaaata tgaactttaa cttttctctt atctttatcg
     4141 acttctttaa tgatacctac aaagttttca aaaggtcctt cgataaccct gatattgtct
     4201 cctacattga agtcaataac tgtttccatt ccttccaatc tcatctgaat gacttcttcg
     4261 gcagtcagcg gaacggaacg cgcggaggga cctacaaaac cggtaactcc tctggtattt
     4321 cttacgacat accatgactg gtcattcata atcattttta caataacata gccggggaag
     4381 atttttttga aggttatttt tctctttccg ttcttttcct caataacttc ctccaagggt
     4441 acaacaatct cctgtatata ttcctgcatt tccctgtttt ccactatttt ttccaggtcg
     4501 gccttaacct tattctcgta accggaataa gtatgcacta cataccatct tgctaaatcc
     4561 tgttcttccc ttttttcggt tatttcagta ttcaaattct cttccattaa aacaatctcc
     4621 cgttttatcc tttaacaagc agtttgataa gggaggataa gccccagtca aacaagctta
     4681 taataatggc aacaatggca catacaacca aaaccgtcac cgtgcttctg aaaagctgtt
     4741 tgcctgtcgg ccatgtaact tttttaagct cctgggcgat tcccttaaag aatcccgtaa
     4801 attttgatcc aaaacttttt ttctcttttc ccgccattgc cgtcacctta ctatttcgtc
     4861 tctttatgag gggtatgctt cctgcagaat ttacagtact tgctgaattc cagtctgtca
     4921 ggagtattct tcttgttttt ctttgtcgag tagtttcttt gtttacactc tgtacatgca
     4981 agtataatct tatctctcat tttctcttcc tgccttttct tattgaagac ttttccacaa
     5041 aaaaaaagag ccttcttata gcaagggata tcttaccatg tctttatata taatgtcaac
     5101 atatttttga caaatacggt ctattcttca gtcaacaata ttcttacggc ctgctttacg
     5161 ttttcaatat taaccgcttc attggatacg cacagtatca tgggaaactc ctcatttata
     5221 tacacatgct ccgaaagaat cggataatcc tgtatattga taccgtatat gactctctct
     5281 cccgtggaag gctttatacc gtaaactata tttttcttat ctatcttaat accatattcc
     5341 tcaatgattt catccaattc cagaagatag ttatatgaca gtatatatct gtacacggca
     5401 tcatcgcata ctattaactc caaaccaccc gatataagca gcaggctcag cttttccaca
     5461 ctggatgcat catatgaatc ttctttgaat cgcacataat tgcccgctgc cgtagcatat
     5521 attgtctcca taagtatctt ctttactttc ggcattctgt catacagata attctccaag
     5581 tctttattgt tcttcggtgt atacggacct atgtaggaaa ttacgaggtc gggatctttg
     5641 ttggtaatat acggaatgac aattgacaat acggtaccta atattatcag cagaattgcc
     5701 gccagaccta tatatttgaa ccatgcttta cggaacttct taacaatggg ttttaccggt
     5761 tcgggttctt caagattccg attcataaga taatcgtacg ccttgataac ttcatcaata
     5821 tctattgact tgtcggtttt cgcacgtaat gacaggttgt cgtacttttt gtttatttca
     5881 gtctcggtgc aatcttcatt aagctccagt aattctaaat atttttttct cagatcatcc
     5941 ataatcgtaa gttaagatta ctattctgtc ttcccttctg tcaaactgct gctttataac
     6001 atcacatcct tagaagaata cccctaagaa catgctacga gcattataac atgttaaagc
     6061 attgtttgtt caaaacaatc aagagacaac taaatacgga ttttactctt ctctatatta
     6121 ttcaaaactt ttgttctcca atataaaatc aatcagatta acatgattaa ttccatttcg
     6181 gtattgtatc aatctgtcgg ttgtcatcac ataacgggga taattgtctc cggtaattgc
     6241 ttcaagactc ctatattccc tatcctctgt cgctttgctc tctaagattg tatatgctac
     6301 ctgaacatat gcatagttca attccgtatc acgcagaata aagtcacact ccagtttgcc
     6361 gatttttcct atacttatgc tatatcccat gctgaaagca tagttatata cgatattttc
     6421 cagtacagga ccgaagttga ttctgttgtc ggtattcatc gcaaagaaga aagataaatc
     6481 cgacagatag tatttttttt cgccacggag agacttcctg gatttcatat caaagcgcct
     6541 gcattccgaa atgattttag cgttttccaa agccttgata tatcgggcaa ttgtctctct
     6601 tttgacagat acattgtttt tccgaaaatc cttaacgata ttttcaatac tggtagtagt
     6661 tccgtaattg ttaacaatat actttaatac cgcatcaaaa acggttctgt ttctaatctg
     6721 aattctgctt cggatatcct tctgatagat ttcttcaatc agtcccgtca cataggctct
     6781 tttgtcctta agagaatcca atcttactgc atatggaaat cctccttcat aaatgtattc
     6841 agaaaggata tcctgtttat ccgccggaac ctctttcccg aaaaacattt tcattcccac
     6901 aaactcttca agagttagcg gcaaaatatt aaattccaaa tatctgcctg taagttttgt
     6961 tacaagctct ccgcttagca gatatgaatt tgaaccggtt ataaaaattg aataatctcc
     7021 ttcttctctc catgagttaa tgactgtttc aaagccctta acattttgaa tctcatcaat
     7081 aaaaaggtat ttcatcccgc ttgccttatt gttcttttca attaagcaat ccaactgttc
     7141 tgccgtattt gttgttgtat acggtcttct gtccaaatta aaaaacagaa tattctcttc
     7201 ttttactcct tgactcaaca gctcacgaac aatcatcttc aagattgatg acttaccgca
     7261 tcgtctgaca ccggtcagaa ccttaataat ttccccctca tgatagaaac cgcgaatttt
     7321 acttaggtat ttttcacgaa aatatagcaa ctcgtcaaaa atttgattca aattctcacc
     7381 tccaatctga aaaaaaaata acacaaaacc ttgcaatatg tcaagttagg gggtatacaa
     7441 ttgattttcg tcaagttata ccccctaatt cattgttttt aataaatata ctgattcata
     7501 ataaaacaag ctaaagatta tccattggac tccggtttca ttgtcgggaa gaacatgaca
     7561 tcccttatcg atgtggaatt ggtaaacagc attatcatac gctctaaccc cagtcccatt
     7621 ccgcctgtcg gaggcattcc cacttccagt gcatttatga agtcatcatc aatggtgaag
     7681 tcgtactccc cgaatttgct cttggcctgc gctataaggc gttctctctg atctacggga
     7741 tcgttaagct cggaaaaggc attgcccatc tcgtttccaa gcacaaagaa ctcaaaacgt
     7801 tcggtaagat gcggtttttc cttgtttctc tttgccagcg gcgaaatttc tatcggatac
     7861 tggtatataa atgtaggctg aataagattc ttttccgcat actcttcaaa gaatgcatta
     7921 agtatcattc cccagttcca atgcttttca atacgcacat tatgcttctt tgcggctgcc
     7981 cttgctgcgg catcatccag tgtgaaagaa tcgaaatcca cacccgaata ttcttttacc
     8041 gcttcaatca tggtaagtct tctccacgga gcttccacat tgataatctg cccctggtag
     8101 tcaatcttac gggtacctga gacattgtcc gctatataac agaatatctg ctccgctctt
     8161 tccatcattg tgtacatatc cgcataggcc tcatataact ccataagagt gaattcggga
     8221 ttatgtcttg tatccatacc ttcatttctg aagactttgc ctatttcaaa tatcttatcc
     8281 aaacctccga caataagtct tttgaggtgc agttccaact caattcttaa atacatatca
     8341 atgttaaggg tattgtgata tgtggcaaaa ggtctcgcat ttgcatttgt agcctgatta
     8401 tgcagtacgg gtgtttccac ttcaagataa cccaagctgt gcatgaattc cctgatggct
     8461 ttcatgatat tagcccttgt tataaatacc gatttaacgt cgggattgac aataagatcg
     8521 acatatcgtt gcctgtaccg caagtccata tctttaagac cgtggaattt ttccggtaaa
     8581 acctgcagtg atttggttaa aagcacaatg cttttcactc ttatggattt ttcacccata
     8641 cgagttataa aagggaatcc ggttacgcct acaatatctc cgatatcgta ttttctgaat
     8701 tccgaatatg tatcttcacc cagttcatca attctgacat agagctgtat tctgtcaaga
     8761 ccgtcctgaa tgtggcagaa acttgctttt cccataattc ttttagccat tattctgccg
     8821 gctaaagaga cttccttatt ctgcagacta tcaaaattgt ccagtatctc ttttgccgta
     8881 tattccctgt caaaacgggt aatgtcgaaa ggatttcttc cttcctgctt aagagcttcc
     8941 agcttttctc tcctgattct taatatttca ttcagatcgg tttcggtgtt ttccactatt
     9001 ttatccattt gtctctcctt caaaacagca gcccttagta taggaactgc tgttaaatta
     9061 taacttattt attgtattta cacaaccgga gtacgggtat tattccgtta tttccagtat
     9121 cttaatctta acggggcccg ccggactttc aaattccact gtttctccgg cttccttacc
     9181 gagtatggct ttacctatcg gagattcata ggaaattctc atattgaacg gatcggcttc
     9241 ggtcggaccg actatctgat atatttcctc ttctttaaat tccaaatcca atatttttac
     9301 tctgcatccg atagttacat tggtggtatc aatattctcg ctgtctatta cttcggcatt
     9361 cataagcaga ttctcaagat acataatccg ggcctccagt tgtgcctgtt cgtttttggc
     9421 ttcatcatac tggctgttct cagcgatatc tccgaatgat attgcactct taagcctgtc
     9481 ggacacttcc atccgtgttt tacctttaag agtagcaagc tcatcttcca attccttgat
     9541 accctcataa gtcatcaaaa cctttttttc tgccatacaa cggtctcctc tcatgcttag
     9601 gattgtgaga ttatattgct ttactacgct tttgtcaacc acctatttcc ctgtgtggga
     9661 aatatgctga atatttaact ttttttatca aaataattac attcaccgcg ggagttttcg
     9721 gataatttcg cgggatatta tgcaattcaa ttgacttgat atgtcttcca taataatatc
     9781 taactgtaag ttttcaaaaa ggggataata ctatgaaaca actgttcaaa gaattggtgt
     9841 tcaaatcgga agccgtttgc acggatttct tcaatgatgt tacttttgat gtaaagttta
     9901 ctcgtcccga cggcagcaat ttattaatac ccggcttctg ggctggagga aatactttta
     9961 aagcaagata tgatatatcc gttcccggaa aacacggcta tgtcaccgtt agcaatgata
    10021 agtctttaaa cggcatatcc ggtcaactgg taatagagaa agaaaaaacc gataatccgt
    10081 actatctgca cggtccggtc tgcagaaaag ggcaaaacat gtatctttcg catagtgacg
    10141 gcactccgtt ctactggttc ggcgatacct ggctgatggg acttacaaaa agacttccgt
    10201 ttcccgatga tttttgcact cttcttaacg acagaaaaga aaaaggcttt aatgttattc
    10261 agataattgc gggactgtat cccgatatga ccatgttcga tccgagagga gaaaacgaag
    10321 cgggttttcc ctggaaagaa gatttatcgg caataaatcc cgagtatttt gacagagccg
    10381 atgaacgtat agaaaaactg gtggagaacg gcattatgcc ttgtatcgta ggcacctggg
    10441 gtttttacat gttttttgcg ggtaaggaaa tacttaaaaa acactggagg tatatcattg
    10501 ccagatgggg agccatgcct gtgtgctggt gtgttgcagg agaagccgac atgcctttct
    10561 ataaccataa tgtaccttat gaagagcata tcaagaaggc aaaaaaagac tggaacgata
    10621 tgacggtatt tataaagcag accgatcctt atgctaggtt aatcaccatt cacccgttcc
    10681 ccgcaagcga cggttatgac cagattgagg acggaagcct tctggatttg aatatgttgc
    10741 agtcgggaca cggcggtttc tcggcgttac cggagcagat gaaaaaagtg cgaaaagcgg
    10801 tggaaagaaa agtattgccc gcaataaatg ccgaagtatg ttttgaaggt atatgcggag
    10861 catcttatgc cgatacccaa aggtatgtgt atctgtcgga ttttctgttg ggagcggccg
    10921 gtcatacata cggagcaaac ggtatatggc aggttaattc cgaagagatg ccgtacggag
    10981 cttctcctca cggcgctcac tgggggaata taagctggag ggaggcgatg aattttgaag
    11041 gagcaaagca gatatcgctc tgcatgaaat atctcacttc ctttgaatgg tggaaatttc
    11101 ggtatcatcc cgaatggctg gaaaccccct gctcattgaa tgcatgtgac ggacattttg
    11161 cagccggtat acccaaaaag gtgcgtgtca tattcaagcc tttcttcgga ggcagcttct
    11221 tcggtgatat aaatatattg aaaatagaaa aaggcatcaa ttacaaagca taccgcttca
    11281 atcccataac ggggaaagaa acatatctcg cagatgtaac acccgacaat gagggaaaat
    11341 ggaaatttcc cagagcggat gctttccagg actggctata tgtcctgaaa gccgaataaa
    11401 atcctttata tacagagata aacggatacg gcactactcc cctattatct gtacctcggc
    11461 tactcttttt ctttcacgaa cctgatccca gtctatgaaa tatatgaatc cgaaatcacc
    11521 gagcgacagt ttgccgtcgg ttattactat tgtttccgaa cgtccgaaga aacaggaccg
    11581 caaatgagca tcggtattca ggcttccttt gggctcttcg ccgggccagg ttaaagcgaa
    11641 ttctatgtgt ttcggtcccg gatgcagata ctggccttca tgcctgcatg tgggcactat
    11701 tttttccatt atgtttataa gatcttgctg taagtgttct ctgccgaaat atgtcgtgtc
    11761 atgcgaacac tcctgagtca ttaccgagca tgtggtatgg tgcgaataaa ccacacagat
    11821 accgttcttt acccccgatt gctccactat cttcttaacc tcatcggtga tgttgtgaaa
    11881 atccggcata tggtcctttg attgcatttc aattgttccc gtgtaaacag tcatttttta
    11941 ttct
//
LOCUS       DAOSOM010000050        11845 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_5361_length_11845_cov_6.397201, whole genome shotgun sequence.
ACCESSION   DAOSOM010000050 DAOSOM010000000
VERSION     DAOSOM010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 11845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11845
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5361_length_11845_cov_6.397201"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="PLF14_08870"
     CDS             <1..692
                     /locus_tag="PLF14_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT71434.1"
                     /translation="MIKKIVQNGELGDVYYIQTGGGRRRGIPTPFGTTFIQKDTGGIG
                     ALGDIGCYSLDMVLNAVGYPKPLTVSGYKSDFFGKRPSYYPKHPEYAKVFGVDDFAAA
                     FIRLEGGIVLDFRISWAMNMDTSGDTLILGTKAGLRIPSTECWNGTVGGPMTLYHEIS
                     GKQVETVIPVIKTDFNLFDMKIRTFLDAIKEGTPSPIPSSQILYNQAIIDGINKSAEL
                     GREIEIKIPKI"
     gene            819..2075
                     /locus_tag="PLF14_08875"
     CDS             819..2075
                     /locus_tag="PLF14_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71435.1"
                     /translation="MRYKIEKREREYLRDYAKKQLEISKLPVMNERVKRWYNHNENRK
                     GKPMVVIELNSFKNDVRPPLKTQSDFARRIEAAIQSNLLVHELLDDDQVCPDYISFNW
                     DITYKEFGVDIPVIKSKDASGREIGFEYIHPITDLERDFPILKPFAFSVDRENSLRRL
                     QAAEDILGDILKVELENTFHQWTNMITMKVIKLMGMENWMIAMFETPDTVHKLMDYIT
                     SNLLAYQQWMESEGLLTMNNGNHYTGAGSRGFTHELKKPADGRVRLKDLWVNVNSQES
                     SGISPDMYKEFVYPYIDRIAREYGMVYYGCCEAVHSLWDVCLKDLSNLRKVSISPWCD
                     EEFMGERLRGTNIIYSRKPSPHFLGVGYDFDDDEYKKYMQNTLKAAKGCNLEIIFRDV
                     YTLGGNPSKAKRAVRILRELIEEYWE"
     gene            complement(2078..3667)
                     /locus_tag="PLF14_08880"
     CDS             complement(2078..3667)
                     /locus_tag="PLF14_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71436.1"
                     /translation="MKELIKERIRRSDDIIENQLNAQCTDPDSVYYGNVPDRTAGYNE
                     PTRSILVAATYITRYCHPLSRFYENQLLLQRALIGLKHGLKHMHWDNTMDLTATNPHD
                     PTSVAFSVRTIVPAFRMLKNYMQEKSSLSALEVKVYNAIINFLTKASDGMVGNGFHTP
                     NHRWVVASGLAMVMNILDMPELMSEINKYLDEGIDCDEYGEYAERSISIYNLTNNESF
                     IILARELNRPEFLEHVRRNLYMSMKYIEPDGTLYTLNSSRQDVGTRIYPFTLFEGYMV
                     MAHIDRNPQFAYMAKVIFDMCSNNPIDTGELGYYCFTAHIIRNMEYAVLFDGEVAIEP
                     FDYTHYDAFFPYSDIARIRDGDTSMTVLAKKAIPVKYMYRFLGIYMMFLSDKYMEFDR
                     IEKTHAGYRLYGNDDRTEVILDCVFQPDKATISVEAKADQPYPCAAVLMFDKEGFLQG
                     DGYSLRARKGKNLHVKNGKFTYLPSVRYIPNSLTVESDFTGDEYSKICTNKVLPTDKN
                     SFTVFLTAEAPFRKTITIKKG"
     gene            complement(3664..5271)
                     /locus_tag="PLF14_08885"
     CDS             complement(3664..5271)
                     /locus_tag="PLF14_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71437.1"
                     /translation="MDEYIDKITDLTNRYVSSITVFTTEKSDSYNKEYIAPSAISIRK
                     TLIITSVCFNPFSRNYRSASLLKTASEYLNNAISLVNKGMYEPVSFCMSVIHASYVYR
                     LAKKHTKALFTHDTHIGKDLQRAALDYIEGAKPLIQSLKEDTVFKYAALIHCARDMED
                     NNLKQCVKEMKERVRDEEYFSVQYLQALCDIHNETKDKQYLKKALNVMTSVLKLTEPD
                     NTVIDLKEEKNNLDKRGSTVYLWDYIDSLMYLSLADNNESLCNVLSYVFNLIDENGCP
                     KSSDNVLLLPVFTLLNKKLFSFILPETTKNKALSSFNGFVGDNVYRYGTGTYISQTVS
                     KEQDTFYTMQCGDLKVRLRFNATYFGPRGRFRSDVLEKTDTGLRLRYIRDWGYWLPLE
                     DRSVPAIIGQDVNKVDRTMTNLQHIDMQADIKFIDNGADITISIKGTDGLATKLDLIF
                     EKHGLFDCEYASTQAAGNDFIQLQQGSFTYTAGSDRITAGPAFNELSIGSEGLRGSNP
                     PIENSFTVYFTGMTPMVHTVRIRGDRT"
     gene            5492..6043
                     /locus_tag="PLF14_08890"
     CDS             5492..6043
                     /locus_tag="PLF14_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOT71438.1"
                     /translation="MIFGYVILGVILLVAVYFIATYNAFIRLRNKYEEAFAAIDVFCK
                     KRYDLIPNLIETIKGYAKHEEQTFVKVVEARAKAMQSKTIAESQANETALEGALKSLF
                     ALAENYPDLKANQGFLDLQAQLTTIEGELAGSRRYYNAIVQSFNTKTQTIPSSFVASI
                     CKFEKQPYYEVAEEARENVKVSF"
     gene            6049..8511
                     /locus_tag="PLF14_08895"
     CDS             6049..8511
                     /locus_tag="PLF14_08895"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOT71439.1"
                     /translation="MNKTGFYRSIVSIALSVLLICAFLQVFAYDVNGYHISDYDIAID
                     VGEDNVMYITENITADFTDSGKHGIIRTIPTVFGNQRVFIDDIYVDKTYVVSTEADYV
                     SIRIGDADKTVKGEVKYKITYRYDYGDDQQDAYDKIYHNILGTDWDCDIYKVTFTVNM
                     PKSFDKEKISITYGDRYSDNKYTGYTVSGNSIKGQLTDLSAYQGITLALELPEGYFVN
                     ERPVTVYDRHGYHISHYETVIDVGENNVMHITENITADFTAYGKHGIIRTLPTVFEGK
                     RVYVDNIYVDKTYVVEHAREYVSIRIGDADKVVEGEVKYKITYDYDYGDDLEDAYDKI
                     YHNILGRDWDCDVYLVTFTVNMPKPFDKEKISITYGDWYSDTRYTGYTVTGNTIKGQL
                     SGLSSYQSVTLALELSEGYFVNERPAAGVRIPVIAIIAPVAVLILVLIITGWNKKGRD
                     EQLVPVPQFYPIEGQSPAEVGYIIDGVINNKDITSLLFYWADKGYLEITEKRWSYEFT
                     RLTVPVFENDYERYMFNKLFEYGDGDTVTTDDLKDNFYSAVPNIKSKLKHVFKGRKEL
                     VSKDSLKARMEYFMLALVPFAVFLIVLFLEFFDMGLLVAFILIYGGFYATLGFLYVYA
                     ENKWNIIGKFKKFLYAVAVVIMLVLTTVIGHAIITDGYYYFNIRTYSIAYFIVAFVTA
                     VSLAYCAAMKKRSEYGQHVLEYYLGFKDFIANAEMDKLKTLIDEDPKYFYNILPYAIV
                     LGLERKWGKKFESIVTQPPDWYHGYSFRTFSTMHMLSGLNSCLARTSSSMVQAPSTSS
                     GGGFSSGGFSGGGGGGGGGSSW"
     gene            8550..10343
                     /locus_tag="PLF14_08900"
     CDS             8550..10343
                     /locus_tag="PLF14_08900"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006211024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HOT71440.1"
                     /translation="MTKKDNIERLKGTLPKVGIRPIIDGRRRGVREELEPVVMGMAYS
                     AAEFISQSLKYPNGLPVECVVADTCIGNVAEAAACAEKFAREGVGLTLSVTRCWCYGS
                     ETMDMDPHMPKAVWGFNGTERPGAVYLAAVLAAHSQKGLPAFGIYGKDVQDANDTEIP
                     EDVKEKLLNFVRAGLAVAYMRGKSYVSMGNVAMGIAGSIANPEFYQKYLGIRNEYVDM
                     SEFIRRMNEGIYNKKEFKTALEWTRKNCKEGKDYNKVPNSRQKKDKEWETCVKMAMIA
                     KDIMTGSDYLTKKGFEEEGCGRNAILAGFQGQRQWTDFMPNGDFMEAILNSSFDWTGI
                     RQPYLIATENDGLNGTAMLFGHLLTAGTAQIFADVRTYWSPDAMKRVTGYTFKGHAKN
                     GIIHLINSGAAALDGTGRCEKNGEPAMKPFYEITEEEAQACLDYTTWGPASLGYFRGG
                     GFSSTFLTRGGMPVTMTRMNLVDGLGPVFQIAEGWTVDIDPKAHNIINLRTDPTWPTT
                     YFAPRLTGEGAFTSVYDVMAKWGANHGVLTYGHVGDRFITLCSMLRIPVNMHNVEEYR
                     IYRPSAWSSFGTTDLEGADYRACKNFGPLYK"
     gene            10439..11674
                     /locus_tag="PLF14_08905"
     CDS             10439..11674
                     /locus_tag="PLF14_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009004751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71441.1"
                     /translation="MALTEKEISVLQETAKKYMEYANLPVQKEKIELWKALNRGKMQR
                     PMVVMDQFPWNELNNEGELDLFVEDPVFRRVERYLKREIYKYKHFPVDMVLDPFIRVP
                     KAIDNTGFGIKVEEETLYASGNVSSHVFKNQLETKEDAEKIKDMIITHDEKETERRFE
                     TASEIFKGIAPVMMEGRTCHLGVWDNLTQFMGVENIYFDLVDEPDKLHLFMERMTTSV
                     IKGIEQTNRLKIYDTNANTCHCSYVYTDELLPDSGMGKESITQNGWAFGLAQLFSSVS
                     PAVTEEFEIPYVTRMAKYFGMIYYGCCDRLDDRLEMVLTIPNLMKVSCSPWSDRDAFA
                     EKIGKKVVMSNKPTPAFVATTTMDEEEIRKDLQWTVDAARKNNVNLEMILKDISTVKC
                     QPERLTRWADIAMDVVNNY"
     gene            11680..11754
                     /locus_tag="PLF14_08910"
     tRNA            11680..11754
                     /locus_tag="PLF14_08910"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11713..11715,aa:Arg,seq:ccg)
BASE COUNT         3641 a         2225 c         2728 g         3251 t
ORIGIN      
        1 agatgattaa aaagatagtg cagaacggag aactgggcga tgtctactac attcagacag
       61 gcggcggcag aagaagagga atacccactc ctttcggtac caccttcata cagaaagata
      121 ccggcggaat aggagctttg ggagatatag ggtgctattc tttggatatg gtattgaatg
      181 ccgtaggtta tccgaaaccg cttaccgttt cgggatataa gtcggacttc ttcggaaaac
      241 gaccgagcta ttatcctaaa catccggaat atgcaaaggt tttcggagtg gacgattttg
      301 cagctgcatt tatccgattg gaaggcggta ttgtattgga cttccgtatt tcctgggcaa
      361 tgaacatgga cacatcggga gacacattga ttttaggtac aaaagccgga ttaagaattc
      421 cttcaaccga atgctggaac ggtacggtcg gcggtcccat gacactgtat catgaaataa
      481 gcggcaagca ggtggagacc gtaataccgg taataaagac ggatttcaat ctttttgata
      541 tgaagataag gaccttcttg gatgcaataa aagaaggaac cccgtctccg ataccttcca
      601 gccagatact gtataaccag gctataatag acggaatcaa taaatccgcc gaactgggaa
      661 gggaaattga aattaagata cctaagattt aataacaaaa cgtttaagtt cagtccaaag
      721 gcgccaaaag gcgcctttgt gtcgtatgac gttttatgaa ttttattgat taacagtatt
      781 tgattggata ttatatttaa cagcggagta tggaaattat gagatacaag atagagaagc
      841 gggaaagaga atatttaaga gattatgcca agaaacagtt ggagatttcc aagttgccgg
      901 taatgaacga gcgggttaaa cgctggtaca atcataacga gaacagaaaa ggtaagccta
      961 tggtagtgat agagcttaac tccttcaaaa atgatgtccg gcctcctttg aaaacacaat
     1021 cggattttgc cagaagaata gaagccgcta ttcagagcaa cctgttggtt catgaacttc
     1081 tggatgatga ccaagtttgt ccggattata tttccttcaa ttgggatata acatacaaag
     1141 agttcggagt ggatataccg gttataaaat ccaaagatgc ttcgggcaga gagataggtt
     1201 ttgaatatat acacccgata accgatctgg agcgggattt tcccatactc aaaccttttg
     1261 ctttttcggt agacagagaa aattccttaa gaagactgca agcagccgag gatatattgg
     1321 gagatatact caaggtagaa ctggaaaata cttttcacca atggaccaat atgattacca
     1381 tgaaggtaat aaagctgatg gggatggaaa actggatgat agccatgttc gaaacaccgg
     1441 atacggtaca taagcttatg gattatataa cttccaatct gcttgcatac caacaatgga
     1501 tggagagcga aggattgctt accatgaata acggtaatca ctataccgga gcgggaagca
     1561 ggggatttac ccatgagtta aagaaacccg ctgacggtcg tgtacgatta aaagacttat
     1621 gggtaaatgt caattcacag gaatcttccg gaatatcccc cgatatgtac aaagagtttg
     1681 tatatccgta tatagacaga atagcaagag aatacggaat ggtatattac ggatgctgcg
     1741 aggctgtaca ttctctttgg gatgtttgtc tgaaggattt atccaattta cgcaaagtgt
     1801 ccatctctcc ttggtgtgat gaagagttta tgggtgaaag attaagaggg acaaatatca
     1861 tttactccag aaaaccttca cctcattttc tcggagtggg atatgatttt gatgacgatg
     1921 agtataaaaa gtatatgcaa aataccttaa aggcggcaaa aggatgtaat ttagagataa
     1981 tattcaggga tgtatatact ttaggcggta acccttccaa agccaaaaga gccgtaagaa
     2041 tcttacggga attgatagaa gaatactggg aataacacta tcctttttta attgttatag
     2101 tctttctgaa aggggcttcc gctgtcaaaa aaacggtgaa agagtttttg tcggtcggca
     2161 gaaccttatt tgtgcatatt tttgaatatt catcaccggt aaaatcgctt tccaccgtaa
     2221 gagaattggg aatatatctg accgaaggca aataagtgaa tttgccgttc tttacatgaa
     2281 gattctttcc ctttcttgcc cggagtgaat atccgtcacc ctgtaaaaat ccttctttgt
     2341 caaacataag aaccgccgca cagggatacg gttgatcggc ttttgcctct acggatattg
     2401 ttgctttgtc gggttggaat acgcagtcca atataacttc ggttctgtca tcgtttccgt
     2461 acagtctgta tcctgcgtgc gtcttttcaa ttctgtcaaa ttccatatac ttatccgaca
     2521 aaaacatcat atatatacct aagaatctgt acatatattt tacgggtata gctttctttg
     2581 caagaaccgt catggaggtg tcgccgtctc ttatgcgggc tatgtcggaa tagggaaaga
     2641 aggcgtcata atgagtataa tcaaagggct cgatggcaac ctctccgtca aagagtaccg
     2701 catattccat attccgtatg atatgtgccg tgaagcaata ataacccagc tcgcctgtgt
     2761 caataggatt gttgctgcac atatcaaata ttactttggc catatatgca aattgaggat
     2821 ttctgtctat gtgggccatt accatatagc cttcaaaaag agtgaaagga tagattctgg
     2881 ttccgacatc ctgtctggaa gagtttaagg tatacaaggt gccgtccggc tcgatgtatt
     2941 tcatggacat atacagatta cgtcttacat gctccaaaaa ttccggcctg ttcaactcac
     3001 gggccaatat gataaacgac tcgttattgg tcagattata tatcgatatg gaccgctcgg
     3061 catattcgcc gtattcatca cagtcaattc cttcatcgag atacttgttt atttcactca
     3121 tcagctccgg catatccaat atattcatta ccattgcaag accgctggca acaacccatc
     3181 tgtgattggg agtatggaat ccgtttccta ccattccgtc ggaggcttta gtcaggaaat
     3241 ttatgatagc attgtagact ttcacttcca gagcggaaag tgacgatttt tcttgcatgt
     3301 agtttttaag catacgaaaa gcaggtacga ttgtccttac ggaaaatgca acggatgtag
     3361 ggtcatgcgg attggtggct gtaagatcca tggtgttatc ccaatgcata tgtttcagcc
     3421 cgtgcttcag acctattaaa gcacgctgca aaagcagttg gttttcatag aatcgggata
     3481 gcggatggca gtatctggta atataggttg cggctaccaa tatggaacgt gtaggctcgt
     3541 tgtatcctgc agtacgatcc ggtacatttc cgtaatatac ggaatcgggg tcggtacatt
     3601 gtgcattcag ctggttttct ataatatcat cggaacgtct tattctttct ttgataagtt
     3661 ctttcatgtt ctgtcacctc ttatcctgac ggtatgtacc atcggcgtca tacccgtaaa
     3721 gtaaacggta aaggagtttt ctatcggcgg attgctgcct ctcagacctt cacttccgat
     3781 ggataattca ttaaatgccg gacctgccgt tatacggtcg cttcctgctg tataagtaaa
     3841 actgccttgc tgaagctgaa taaaatcatt gcctgcagcc tgtgtggaag catactcgca
     3901 atcaaacaag ccgtgttttt cgaatataag gtccagcttg gttgctaaac cgtccgttcc
     3961 cttaattgat atggttatat ccgctccgtt atctataaac tttatatcgg cctgcatatc
     4021 tatatgttga agattggtca ttgttctgtc gactttattg acatcctgac ctataatcgc
     4081 aggaaccgat cggtcttcca gcggtagcca atacccccaa tcccttatat acctcaacct
     4141 caatccggta tcggtttttt ccagcacatc gcttctgaag cgtcctctcg gcccgaagta
     4201 tgtggcattg aaccgaaggc gtacctttaa atctccgcac tgcatggtgt agaaagtatc
     4261 ctgctctttg gatactgtct gagatatata cgtaccggtt ccgtaacgat aaacattatc
     4321 gccgacaaac ccgttaaatg aggataaagc cttgtttttt gttgtttcag gtaaaatgaa
     4381 gctgaaaagc tttttgttta acaaagtaaa aacaggcagt aaaagcacat tgtcactgct
     4441 tttcggacaa ccgttttcat caattaaatt aaatacatag gaaagaacat tgcagagact
     4501 ctcattgttg tctgcaagag acagatacat caaagaatcg atatagtccc aaagataaac
     4561 agttgaacct cttttgtcga gattattttt ttcttctttc aagtctatta cggtattgtc
     4621 cggctccgta agtttaagca ccgatgtcat aacattcaat gcctttttta agtattgttt
     4681 gtctttggtt tcgttgtgta tgtcgcaaag tgcctgtaaa tattgaacgg aaaaatattc
     4741 ttcatcccgc accctttctt tcatttcttt gacgcattgc ttcaggttgt tatcttccat
     4801 atctcttgcg cagtgaatca gtgccgcata tttgaaaacg gtatcttctt tcaaactttg
     4861 tatcagcggt ttagcaccct ctatataatc taaagctgct ctctgtaagt ctttgcctat
     4921 atgtgtatcg tgggtaaaca atgctttcgt atgtttcttt gccaatctgt atacatacga
     4981 tgcatgtatg actgacatac aaaaagatac aggttcgtac atgcctttat taacaagtga
     5041 aattgcattg ttaagatatt ccgaagctgt ttttaacagt gatgccgacc ggtaatttct
     5101 tgaaaaagga ttaaaacaaa cacttgttat aatcaatgtt tttctgatgg atattgccga
     5161 aggagcaatg tattctttat tgtaagaatc ggatttttcc gttgtaaaaa cagttattga
     5221 cgagacatat ctgttggtaa gatcggttat tttatcgata tattcatcca taggttactc
     5281 actttcaagg tgattatagc acaagcgatt tattcgtcaa catcaaaggg atgtgccaac
     5341 tctaaaggaa aattttgcat atatttcggc atgtccgcat cttaataact tgtaatagta
     5401 aaagagcata taactttatc aaaaattcat ttgtatgatt atagttgtta atatataata
     5461 aaaacaaacg gaggtaataa taatgggatg gatgatattc ggctatgtaa tattaggagt
     5521 tatactgctg gtagcagtgt attttatcgc tacgtataac gcatttatac gcttaaggaa
     5581 caaatatgag gaagcatttg ccgcaatcga tgtattctgt aaaaagcgat atgatttgat
     5641 acccaatctt attgagacga taaaaggata tgcaaaacat gaggagcaaa cttttgtcaa
     5701 agtagtggaa gccagagcaa aagcaatgca gagtaaaacc attgccgaat ctcaggcaaa
     5761 tgaaacggca ctggaaggtg ctcttaagag tcttttcgca ctggcggaga actatccgga
     5821 tttaaaagcc aatcagggat ttttggattt gcaggcacag ttaaccacta ttgagggaga
     5881 gcttgcaggt tccagaaggt actacaatgc aattgtacag tctttcaata ccaagacaca
     5941 gacaatcccc agttcctttg tggcatctat ttgcaaattt gaaaaacagc cttactatga
     6001 agttgcagag gaagcacgcg aaaacgtaaa agtgagcttt taagaggcat gaataaaaca
     6061 ggtttttata ggagtatagt atcaatagcg ctgtcagttt tactgatatg cgctttttta
     6121 caggtttttg cgtatgatgt gaacggatac catatttccg attatgacat tgcaatagat
     6181 gtaggtgaag acaatgtaat gtacataacg gaaaatatta cggctgattt taccgacagc
     6241 ggcaaacacg gtattatacg tacaattccc accgttttcg ggaatcagag agttttcatc
     6301 gacgacatat atgttgataa aacctatgta gtgagtactg aagcggatta tgtttccata
     6361 cgtataggtg atgccgacaa aaccgttaaa ggtgaggtta aatacaaaat aacttatcgt
     6421 tacgattacg gtgatgacca acaagatgct tacgataaga tataccataa catattggga
     6481 accgactggg actgtgatat ttataaagtc acatttactg taaatatgcc caaatcgttt
     6541 gataaagaaa aaataagcat tacatacggt gacaggtaca gtgataataa atatacggga
     6601 tataccgttt cgggaaacag cataaaagga caactgaccg acctgtccgc ttaccaggga
     6661 attactctgg ctttggagct tcccgaagga tattttgtaa acgaacgtcc cgtaacggtg
     6721 tatgacaggc acggatatca tatttcgcat tatgaaaccg ttatagatgt aggtgaaaac
     6781 aatgtcatgc atataacgga gaatattacg gctgatttta ccgcctacgg caagcacggt
     6841 ataatacgta ctcttcccac cgtttttgaa ggcaagaggg tttatgtaga caacatatat
     6901 gtggacaaga catatgtagt ggagcatgca agagaatatg tttccattcg cataggcgat
     6961 gccgataaag ttgtcgaggg cgaggttaaa tacaaaataa cctatgatta cgactacggc
     7021 gacgacttgg aagatgcata cgataagatt taccataata tactgggaag agactgggac
     7081 tgcgatgttt atctggttac tttcacggtt aatatgccta aaccgttcga caaagagaaa
     7141 ataagtatta catacgggga ttggtatagt gacacaaggt ataccggata taccgtaacg
     7201 ggtaatacta taaaaggaca actgtccggt ctgtcttcct atcaatcggt tacacttgcg
     7261 ctggaacttt ctgaaggata ttttgtaaac gaacgtcccg cagcgggtgt aaggattccg
     7321 gtcatagcga taattgctcc ggtagcagta ctgattctgg tgctcattat aacgggctgg
     7381 aataaaaaag gaagggacga acaacttgtt cccgtaccgc agttttatcc catcgaagga
     7441 cagtctcctg cggaagtggg ctatataatc gacggagtta tcaataacaa agatattact
     7501 tctttactgt tctactgggc ggacaagggg tatctggaga taacggaaaa acgttggagc
     7561 tatgagttta ccaggctgac ggtaccggta tttgaaaatg actatgagcg gtatatgttc
     7621 aacaaactgt ttgaatacgg agacggtgat acggttacca ctgacgattt gaaggataat
     7681 ttctattccg ccgttcccaa tataaagagc aaattgaaac atgttttcaa aggaaggaaa
     7741 gaactggttt ccaaagactc attaaaagcc cgcatggaat attttatgct ggctttggtg
     7801 ccttttgcgg tatttctgat agttctgttt ctggagttct ttgatatggg gcttttagta
     7861 gcatttatat taatttacgg cggtttttat gcaaccctcg gtttcttata cgtttacgcg
     7921 gaaaacaaat ggaatataat cggcaaattc aaaaaattcc tgtatgcggt agcggtggta
     7981 atcatgttgg ttttaacaac cgtaatcggt catgcgataa ttaccgacgg gtactactac
     8041 tttaacatca ggacctactc aatagcatat ttcatagtcg cttttgtaac tgccgtatcg
     8101 ctggcatatt gtgctgcaat gaagaagcgt tcggagtacg gacagcacgt tctggagtat
     8161 tatctgggat tcaaggattt tatagccaat gccgaaatgg acaagttaaa aacacttatt
     8221 gacgaagatc cgaaatattt ttacaacata ctgccttatg caattgtgtt gggtctggaa
     8281 agaaaatggg gtaaaaagtt tgaaagcata gttacacaac cgcccgattg gtatcacgga
     8341 tacagtttta gaacattctc cacgatgcat atgctgtcag ggttgaacag ctgtctcgcc
     8401 cggacgtcat catccatggt tcaggcgccc agcaccagca gcggcggcgg cttctcttcg
     8461 ggaggattct ccggcggcgg cggcggtggc ggcggcggtt ccagctggta ataaataatt
     8521 gatgaatata tatggaagga gtaaaggaaa tgacaaagaa agacaacatt gagagattga
     8581 aaggtactct gccaaaagtc ggtataagac ctattattga cggaagaaga agaggcgtca
     8641 gagaggaact ggagcctgtt gtaatgggaa tggcctattc ggcagcggaa ttcatttcac
     8701 agagtttaaa atatcccaac ggactgcctg tcgaatgtgt tgtagcagat acctgcatag
     8761 gaaatgtggc ggaagctgcg gcttgtgcgg agaaattcgc aagagaagga gtaggactta
     8821 cactttcggt tacgcgttgc tggtgttacg gaagcgagac aatggatatg gatccccata
     8881 tgcccaaagc tgtctgggga tttaacggta ccgaaagacc gggagccgta taccttgcag
     8941 cggttttagc agcacacagt cagaaaggat tgcccgcatt cggaatatac ggaaaagacg
     9001 tacaggatgc caatgatacc gagataccgg aagatgtaaa ggaaaaattg cttaatttcg
     9061 taagagcggg acttgccgta gcctatatga gaggtaaatc ctatgtcagc atgggcaatg
     9121 ttgcaatggg tatagccggc tctattgcca atcccgagtt ctatcagaaa tatttaggta
     9181 taagaaacga gtacgtggat atgtcggagt ttatccgcag aatgaacgaa gggatataca
     9241 acaagaaaga atttaagacc gctttagagt ggacaagaaa gaactgtaag gaaggcaaag
     9301 actacaacaa ggtacccaat tcccgtcaga agaaagacaa agagtgggag acctgcgtaa
     9361 aaatggccat gattgcaaaa gacattatga ccggaagcga ctatctcaca aagaagggat
     9421 ttgaagaaga aggttgcgga aggaatgcaa ttttagcggg attccaggga cagaggcaat
     9481 ggaccgactt tatgcccaac ggagacttta tggaggccat actgaactcc tcatttgact
     9541 ggaccggaat acgtcagccc tatttgattg caaccgagaa tgacggactt aacggaactg
     9601 caatgctttt cggacatctc ctgacagcag gtaccgcaca gatatttgcc gatgtcagaa
     9661 cctactggag ccccgatgct atgaagaggg ttaccggtta tacatttaaa ggacatgcca
     9721 agaacggtat tatccacctc ataaactccg gagcggcagc tttggacggc accggcagat
     9781 gcgaaaagaa cggcgagccc gccatgaaac ctttctatga aataaccgaa gaagaagcac
     9841 aagcatgcct ggattatacc acatggggac cggcaagctt aggatatttc agaggaggcg
     9901 gtttctcttc cacattctta acaagaggcg gaatgcccgt taccatgaca agaatgaatc
     9961 tggtagacgg tctcggaccg gtattccaga ttgcggaagg ctggacggtg gatatcgatc
    10021 ccaaagcaca taacattatt aacttaagaa cagacccgac atggcctaca acatattttg
    10081 caccgcgcct cacgggcgaa ggagctttca ccagtgtata cgatgtaatg gctaaatggg
    10141 gagcaaacca cggagtgctg acatacggac atgtgggaga ccgttttatc acattgtgtt
    10201 ccatgttaag aataccggtg aatatgcata atgttgaaga gtatagaata tacaggccca
    10261 gtgcatggtc atccttcgga acaacagatt tagaaggtgc cgactacaga gcatgtaaga
    10321 acttcggacc tctgtataag taatatattt tcaatatccg agttaacagg caatttttac
    10381 atatacttta ataaatatta aggtataatt tagtatgcat atatggaggg agagtgctat
    10441 ggctttgact gaaaaagaaa tatccgtttt gcaggaaacg gcaaagaaat acatggaata
    10501 tgcaaatctt ccggtccaaa aagaaaagat agaactttgg aaggcattga acagagggaa
    10561 aatgcaacga cccatggttg taatggacca atttccctgg aatgagctta acaatgaagg
    10621 cgaactggat ttgtttgtgg aggatcccgt tttccgaaga gtagagcgct atttgaaaag
    10681 ggagatatat aagtataagc actttcccgt ggatatggtt ttagacccgt ttatacgggt
    10741 tcccaaagcc atagacaata ccggtttcgg tataaaagtg gaagaagaaa cgctgtatgc
    10801 atcgggcaat gtcagttccc atgtttttaa aaaccaactg gaaacaaagg aagatgctga
    10861 aaagattaaa gacatgataa taacccatga cgagaaagag accgaaagaa gatttgaaac
    10921 ggcttcagaa atattcaaag gtattgctcc ggtaatgatg gaaggcagaa cctgtcatct
    10981 gggtgtatgg gataatctca cgcagttcat gggagtggag aatatatatt tcgatttagt
    11041 agacgagccg gataagcttc atctgtttat ggagagaatg accacttcgg ttataaaggg
    11101 aattgagcag acaaacaggc ttaagatata tgatacaaac gcaaatacct gccattgctc
    11161 ctatgtgtat acggatgaat tgctgcctga cagcggaatg ggtaaagaaa gcattacgca
    11221 aaacggttgg gcattcggcc ttgcacagct gttctcctcg gtatcaccgg cagtgaccga
    11281 agagttcgag attccctatg taacaaggat ggcaaaatac ttcggtatga tatactacgg
    11341 atgctgtgac cgattggacg acagactgga gatggtactg actataccca acttaatgaa
    11401 agtatcctgc agtccctggt cggacagaga tgcatttgca gaaaagatag gcaaaaaagt
    11461 ggtaatgagc aacaagccca ctcctgcatt tgtcgccaca accacgatgg atgaagagga
    11521 gataaggaag gatcttcagt ggacggtaga tgcggcaaga aagaacaatg taaatctgga
    11581 aatgatactg aaggatatat ccacggtgaa atgtcaaccg gaaagactga caaggtgggc
    11641 tgacatagca atggatgtgg taaataacta ctaaagattg tcccggtagt ataatggata
    11701 aaacggggga ttccggttcc tcagatgcgg gttcgattcc tgttcgggac gccataaaag
    11761 aaacgtctat tgcctaaaag acgtttcttc tcgtgtatga cgggcatgtt gtcagtctgt
    11821 ggtgaaagtc cacaaggggc gtagt
//
LOCUS       DAOSOM010000051        11526 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_5583_length_11526_cov_5.687124, whole genome shotgun sequence.
ACCESSION   DAOSOM010000051 DAOSOM010000000
VERSION     DAOSOM010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 11526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11526
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5583_length_11526_cov_5.687124"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2232)
                     /locus_tag="PLF14_08915"
     CDS             complement(<1..2232)
                     /locus_tag="PLF14_08915"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HOT71442.1"
                     /translation="MYKDQDHIRKFLNDMDIMRFTDVTDVSDIYIKEVPGKINLMALK
                     ETDISAMQPYKSGTIYETEGNKYHIFKFKLRVSPVYDRKPFVLKAITGFGTDWNVFRN
                     PNFLLFINGQPEQAFDFGHRECIITDCAKAHDEYECFLFEFSGMKKGPVQLTLQTCSL
                     DYPTDELYYNILCPGESIPFIESEAIRKDTAKYLINAINMVDLREPYSDKYYETVSKA
                     NGYLLDEYYGKYCDDDTSEVTVAAVGHSHIDVAWRWPIKETRQKTVRTFTSQLKNIRD
                     YKDFRFIVSQPQLYKYLEEDYPELIDKIKPAVESRQWEPEGAMWVEADCNLTSGESLV
                     RQIMHGKKYFKDMFDRDCRILWLPDVFGYSAALPQILKKSGIDYFSTSKISWNEYNVI
                     PYNTFMWEGIDGTEIFTNFIVGGKGPNEEGRPRVTYNSWVNAEQIMGTWKNYRDKSLA
                     KYPFMSYGLGDGGGGPGRDHLEQAIRFSRGIKGMPRFESVTVTEYFDKLYDLVKDDSR
                     LPKWVGELYLEFHRGTYTSVAKNKKNNRKSEILMRDTEAFCSLADRFGLDTYDKKKIY
                     DTWETILLNQFHDIIPGSSIKEVYDDSDADYARVMKDGKELLSGSLNAFAKTVKGQDL
                     CVVFNQLAFKRVDTVKLRTDKDFTCVYDISGERIPGQTVIEDNKKYFLFEARLCSMGY
                     TIFRLSDEKCIVDDNSFVATCNMMENKHLKVVFDDFMNISSIYDKDAKREVVQKGQTA
                     NKIT"
     gene            complement(2242..3426)
                     /locus_tag="PLF14_08920"
     CDS             complement(2242..3426)
                     /locus_tag="PLF14_08920"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT71443.1"
                     /translation="MKIVVVGIGGYGEVVLDGLFENPCNLDYSIAGYVAPRPKDNDYS
                     KRLHEMGAQRYATLEEFFEHKHADLAVISSPIEFHMPQTILCLQHNTAVMCEKPVAGT
                     IQDAYAMKKAEEASQAFVGIGYQWSYSDAIVNLKKDILAGMYGKPVRLKNIVLWPRTQ
                     RYFKRNSWAGKIKNDSGYWILDSVANNATAHYIHNMFFVLGDGLKSSAWPETIQANIY
                     RANDIENYDTCVCRVITQKGVEILYYASHSTDIKCEPLLHFQFEKGEIYADFNKEKCI
                     YGKVGGKVISYGNPFELEMKKVFDCIRTLKGADVNYCPIEAAIPQLKTINAISQFYDI
                     EQVEESDLGYLDAEENTTKLVYVKGLYQDMMKAYSDNLILDESAKVIDITGYKYFENM
                     KQ"
     gene            complement(3426..4397)
                     /locus_tag="PLF14_08925"
     CDS             complement(3426..4397)
                     /locus_tag="PLF14_08925"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT71444.1"
                     /translation="MKKVRVGLYGINGHQIHNQMVNHEKAQVTAISMIDREKLPKELL
                     TGDVVFYEDFDSMLKAKNVDVISICAPRRIDQKDLAVKALLAGKHVYSEKPCAYTEKD
                     LDEILKVSKKTGMLFHEMAGSAFEEPFISVTELIKSGRIGEVVQVFAQKSYPFKTSAG
                     RPQDDITDGGLIRWIAVHAARFVEHTTGLKIKDIYAMETRHGNPVKDGNLKMAASIMF
                     SLDNNAVGCIIANYLNMNNFPTWGNEHLRVWGTDGFVEITDGLTATRLVTPDYDGPVE
                     IKYATRDYFDFFIDEILYNKPMPLTLEEELHPLRAVIRAQEKAVEVQ"
     gene            complement(4475..6493)
                     /gene="uvrB"
                     /locus_tag="PLF14_08930"
     CDS             complement(4475..6493)
                     /gene="uvrB"
                     /locus_tag="PLF14_08930"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOT71445.1"
                     /translation="MSRRDKEFRSDKFVLQSDFRPTGDQPAAIEALANGVLSGYKHQT
                     LLGVTGSGKTFTMANIIEKVQKPTLVIAHNKTLAAQLCSEFKEFFPDNSVEYFVSYYD
                     YYQPEAYIPSTDTYIEKDMSINDEIDQLRHAATSALLQRRDVIIVASVSCIYGLGSPA
                     DYEDLMLYIGVGMTLDRDDVIKKLVEIQYSRNEMDFKRGTFRAKGDSLEIAPSNSANV
                     GYRISFFGDEVEKITQFDSLTGEITGYVNHLAVFPASHFVSKDTKLKRAIEDIQAELQ
                     ERVAYFENQGKIVEAYRLNQRTSYDLEMIRNIGFCSGIENYSRHIDGRKEGQPPATLL
                     SFFPEDFLMFIDESHVTIPQIGGMNKGDRSRKKNLIDFGFRLPSAYDNRPLTFEEFKE
                     KQNQCIYVSATPGDYELGISEQVAEQIIRPTGLTDPKIEVRPVKGQVDDLLFEIRDTV
                     SRKNRVLVTTLTKRMAEKLTEYYSSLGVSVQYLHSDVETLERIEILKQLRDGTFDVLI
                     GINLLREGIDLPEVELVAVLDADKEGFLRSARSLIQTAGRAARNVAGRVIMYADTITD
                     SMKVAINETNRRRAIQTEYNEKHNITPVGIVKEIRDNIRFRIAEKTDTKYSHVFEIPK
                     TEVEIIRLIDLYERQMKRYAKLQEYENAARYRDMLKELKEALQQGDVY"
     gene            6608..6928
                     /locus_tag="PLF14_08935"
     CDS             6608..6928
                     /locus_tag="PLF14_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009446011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT71446.1"
                     /translation="MRGINERIKKLRTQMNLSQDYVAKYLGVSRSTFTQMENGNRKIL
                     ADEISKLSTLFGVSVNAILDESELSQPATVFARSFEKLDESDQAEIMNLIRFKEQMKA
                     QRTK"
     gene            7030..7848
                     /locus_tag="PLF14_08940"
     CDS             7030..7848
                     /locus_tag="PLF14_08940"
                     /inference="COORDINATES: protein motif:HMM:NF018406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HOT71447.1"
                     /translation="MENKRKPVFWIFIKSFTDMHAYKHFVYKNFSKAFLYILLFTFLL
                     SVIFSAIFVASIKDYTTQLITQLEQNTPEFTIKNGELVIESDQPVILAETDDKGTLII
                     IDKEYTGSVTKYEEYDTVIVLGQKEIYLSNSAIRYSIGYQSVLGQKQEVTSEMLFGRV
                     SSSKPYLYIFFFVSMFVFFIGNYLFYDLFIALIVNTIRRIRKRPANLGISLQLTAYAL
                     TLPAIIYLIWIFTSLITIPIEPLIFILAVIIAYKGVDAYYKSIDEELTKPKEEA"
     gene            7929..8105
                     /gene="rpsU"
                     /locus_tag="PLF14_08945"
     CDS             7929..8105
                     /gene="rpsU"
                     /locus_tag="PLF14_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOT71448.1"
                     /translation="MAEVRLKENETLDSALKRFKRQCAKAGILAEVRKREHYEKPSVK
                     RKKKSEAARKRKKR"
     gene            complement(8245..8592)
                     /locus_tag="PLF14_08950"
     CDS             complement(8245..8592)
                     /locus_tag="PLF14_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HOT71449.1"
                     /translation="MENCIFCKIVRKEIPSTKVYEDDYVYAFKDINPQAKHHVLVIPK
                     QHILSDINDITEDNCFIIGRMFYAAKKISEILGIEKSGVRIINNTGKDAHQTVKHIHI
                     HVLGGQDMGEGLV"
     gene            complement(8592..11186)
                     /gene="alaS"
                     /locus_tag="PLF14_08955"
     CDS             complement(8592..11186)
                     /gene="alaS"
                     /locus_tag="PLF14_08955"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOT71450.1"
                     /translation="MEKLSLNTLREEFLKFFESKGHLRLKSFSLIPEDDPSILLINAG
                     MTPLKPYFTGALKPPSKRITTCQKCIRTLDIDRVGITTRHGTFFEMLGNFSFGDYFKK
                     EAIQWSWEFFTEVLKMDKNLLYASVYEEDDEAYDIWKNIVKLDESHIVRMGKEDNFWE
                     HGTGPCGPCSEIYYDRGEDKGCGSKDCKVGCDCDRFVEVWNNVFTQFDKQEDGTYKPL
                     KNKNIDTGMGLERLACVVQGVDSIFEVDTVKDILDFVCNATSTEYGKDQTKDISIRVI
                     TDHIRSSVMMISDGIIPSNEGRGYVLRRLLRRASRHGRLLGKKDAFLSELIDVVINNS
                     KSAYPELEEKRDYILKVVQSEEAKFINTIENGMNILNQYLEEAENNKKDTLAGEHIFK
                     LHDTYGFPYDLTREIASERGFKLDKEGFDEYMQNQKKTARDAFKADEGSAWHSDENIY
                     KNIPKTEFSGYEKLEIKAEVLSFVKKDETAELVTDKTVFYAEGGGQQADIGVITGKKA
                     KMTVTDVINREGIFIHRGVMEQGSFSDDETVTLKVDKSRRKATERNHTATHMLHYALR
                     EVLGNHVHQAGSYVDHQRLRFDFTHFSAMDDRQLMKVVSIVNNIIMEDINVVKEIMDM
                     DTAKKKGATALFGEKYSDKVRVVSIHPYSMELCGGTHLSKTGETGLFTIVSEGAVASG
                     VRRIEALTGTNAIEYMQKLEDTVNQLNRMLKGSDVIGRVENLSSTVRELRKENEELKN
                     KLSAHKLDNVLNTFEQYKNIKVVSAVIENADMQVLRNLSDKARERYDDCVCVFVSTGE
                     KVSMVIAATKKAVTSGVNAGTVIKEAAALIGGSGGGRPDMAQAGGKDSDKAHDAVQKA
                     VQLIKEAI"
     misc_binding    complement(11228..11427)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         3076 a         2693 c         1991 g         3766 t
ORIGIN      
        1 cgttattttg tttgcggtct gacctttctg taccacttcc ctctttgcat ctttgtcata
       61 aattgaagat atgttcataa agtcgtcaaa cactacctta agatgcttat tctccatcat
      121 attacatgtt gcgacaaatg aattgtcatc aactatgcac ttttcatcgg aaagacggaa
      181 tatagtatac cccatggagc acaatcgcgc ttcaaacagg aaatattttt tattgtcttc
      241 aataacggtt tgtccgggaa ttctttcgcc gcttatgtcg tatacgcagg taaaatcctt
      301 gtcggttctt aactttacgg tatccacacg cttgaaagca agttgattaa atacaacgca
      361 caggtcctgc cctttaacgg tctttgcaaa agcattgagc gagcctgaca gaagctcttt
      421 accgtctttc atgactcttg cataatccgc atcggaatcg tcatatactt ctttaatgga
      481 ggaccccggt attatgtcat ggaactggtt taacagtatt gtctcccagg tatcataaat
      541 cttcttcttg tcgtatgtat ccagcccgaa tctgtcggct aaagagcaga aagcctcggt
      601 atcacgcata agtatctcgg actttctgtt attcttctta tttttggcta ccgatgtata
      661 tgtaccgcga tgaaattcca gatacagctc tcctacccat ttaggcagtc ttgaatcgtc
      721 ttttaccaag tcatacagtt tatcgaaata ttcggtcacc gttacactct caaatctcgg
      781 catacctttt atacccctgg aaaatcggat agcctgctca agatgatctc tgcccggtcc
      841 cccgccgccg tcgcccaatc cgtatgacat aaacggatat ttagctaagc tcttatcccg
      901 gtagtttttc catgttccca ttatctgctc ggcatttacc caggagttat atgttactct
      961 cggtcttcct tcctcattgg gaccttttcc gcccactata aaattggtga atatttccgt
     1021 tccgtctatt ccttcccaca tgaatgtgtt atagggaatg acattatact cattccagga
     1081 tattttggat gtggaaaaat agtctattcc cgatttcttt aaaatctgag gcagtgcggc
     1141 actgtacccg aaaacatcgg gaagccataa aatcctgcag tccctgtcaa acatatcttt
     1201 gaaatatttt tttccgtgca ttatctgccg cacaagagat tctccgctgg taagattaca
     1261 gtcggcttcc acccacatcg caccttccgg ctcccactgt ctgctctcca ccgccggctt
     1321 tatcttatct atcaattcgg gataatcctc ttccagatat ttatacagct gaggttgact
     1381 tactatgaac ctgaaatctt tgtaatcacg tatattcttc aattgtgatg taaaggttct
     1441 cacggtcttt tgtctggttt cttttatagg ccatctccag gccacatcta tgtgcgaatg
     1501 tcctacagcg gcaacggtaa cttccgatgt gtcatcatca caatatttac cgtaatactc
     1561 atccaaaaga tacccgtttg ctttacttac ggtttcataa tatttatccg agtaaggttc
     1621 tctcaaatcc accatattta tggcgttaat caggtatttt gcagtgtctt ttcttattgc
     1681 ttcgctttca atgaacggaa tggactctcc cggacagagg atgttgtaat aaagctcgtc
     1741 ggtgggataa tccagcgagc aggtctgtaa tgtaagctgt accggtcctt tcttcattcc
     1801 ggagaactca aataaaaaac attcatactc gtcatgtgcc ttggcacagt cggttattat
     1861 gcattcacga tgtccgaagt caaaagcctg ctccggctgt ccgtttataa aaagcaggaa
     1921 gttgggattc cgaaaaacat tccaatcggt cccgaatccc gttatagctt taagaacaaa
     1981 cggttttcgg tcatatacgg gacttactct taatttaaac ttgaatatgt gatatttatt
     2041 accctctgtc tcatatatgg taccgctttt atacggttgc attgcagaaa tatcggtctc
     2101 tttcaatgcc atcaggttta tcttgcccgg cacttccttt atataaatgt cggaaacatc
     2161 ggtaacatcg gtaaacctca taatatccat atcattaaga aacttcctta tatggtcctg
     2221 atctttgtac ataataccct cctattgctt catattctca aaatatttat atcctgtaat
     2281 atcaataact tttgccgact catcaagaat caaattgtcg ctgtatgcct tcatcatgtc
     2341 ttgatacaac cctttgacat ataccagttt ggtggtattc tcctcggcat ccagatatcc
     2401 taaatcactt tcctccacct gctctatgtc ataaaactgg ctaattgcat ttattgtttt
     2461 aagctgcggt atagccgcct ctatggggca ataatttaca tcggcccctt ttagtgtcct
     2521 gatacagtca aataccttct tcatctccaa ctcaaacgga tttccgtagg atattacctt
     2581 accgcctact tttccgtata tgcatttttc cttattgaaa tcggcatata tctcgccttt
     2641 ttcaaactgg aaatgcaaca gaggttcgca ctttatatcg gtggaatggc ttgcataata
     2701 caatatctcc actccttttt gtgtgattac cctacaaacg catgtatcgt aattctcaat
     2761 atcatttgct ctgtatatgt tggcctgtat ggtttcaggc catgcactgc tttttaaacc
     2821 gtcacctaaa acaaagaaca tgttgtggat atagtgtgcc gtggcattgt tggccaccga
     2881 atccaatatc caatatcccg aatcattctt tatctttccg gcccagctgt ttcgcttaaa
     2941 atatctttga gttctgggcc aaagcactat attcttcaaa cggacaggtt ttccgtacat
     3001 gcctgcaaga atatctttct ttagatttac aatggcatcg gaataggacc actgatatcc
     3061 tattcccaca aatgcctgcg atgcttcttc cgccttcttc atggcatatg catcctgtat
     3121 tgttcccgct accggctttt cgcacataac agcggtgtta tgctgcaggc ataagattgt
     3181 ctgaggcatg tgaaactcta tgggactgga tataaccgca aggtccgcat gtttatgctc
     3241 aaagaactct tccagcgttg catacctttg tgctcccatt tcatgcaacc ttttggaata
     3301 atcattgtct ttgggtcttg gagcgacata tcccgctata gagtaatcca gattacaggg
     3361 attttcgaac aacccgtcaa gcaccacttc accgtaaccg cctataccta ccactacaat
     3421 tttcattact gcacctccac cgctttttcc tgtgctctta tcaccgctct taacggatga
     3481 agttcctctt ccagcgtcaa aggcataggt ttgttatata aaatctcgtc tatgaaaaag
     3541 tcaaaatagt cccgagttgc atacttaatc tctaccggcc cgtcataatc gggagtaacc
     3601 agcctggtgg cggtcaatcc gtcggttatc tccacaaaac cgtcagttcc ccatactctt
     3661 aaatgctcgt tgccccatgt agggaaattg ttcatattaa gataattggc tataatgcat
     3721 cctaccgcat tgttatctaa agaaaacata atacttgccg ccatcttgag attgccgtct
     3781 tttacgggat ttccgtgccg cgtttccatt gcatatatgt ctttaatctt caaacctgtt
     3841 gtatgttcca cgaatcttgc ggcatgaacg gctatccagc gtataaggcc gccgtcggta
     3901 atgtcatcct ggggtcgccc cgccgatgtt ttaaaaggat aggatttctg tgcaaacacc
     3961 tgaacaacct cacctattcg tcccgattta attaactccg taaccgaaat gaaaggctcc
     4021 tcaaatgccg atccggccat ttcatggaaa agcataccgg tcttttttga taccttaagt
     4081 atttcatcca aatccttttc ggtataagca caaggttttt cactgtaaac atgcttaccc
     4141 gcaagcaggg cttttacagc taaatccttc tggtctattc tcctgggagc acatattgaa
     4201 ataacatcaa cattctttgc tttaagcatg ctgtcaaaat cctcatagaa cacaacatcc
     4261 ccggttagaa gttccttagg taacttctct ctgtctatca tggaaattgc ggtcacttgc
     4321 gctttttcat ggtttaccat ctggttgtgt atctgatgtc cgttaatgcc gtaaaggccg
     4381 actcgtactt ttttcatatt aattccgggg tactttctcc ccttctcctt tcaggttccg
     4441 ccacatttaa tcttatccca tttccggctt cttttcaata tacatcgcct tgctgcaatg
     4501 cctctttaag ctctttcaac atatctctgt atcgtgcggc attttcatat tcctgaagtt
     4561 ttgcataccg cttcatctga cgctcatata aatcaattaa acgtattatc tccacttcgg
     4621 tttttggtat ttcaaaaaca tgcgaatatt ttgtatcggt tttttccgct atcctgaaac
     4681 gtatattatc ccttatctct ttaacaatac ctaccggagt aatattatgc ttctcgttat
     4741 actcggtctg tatcgctctt cttctgtttg tttcattaat ggcaaccttc atgctgtcgg
     4801 taattgtatc tgcatacatt attaccctgc ctgcaacatt tcttgcagct cttccggctg
     4861 tctgaataag agaacgtgca cttcttaaaa atccttcttt atcggcatcc aacaccgcca
     4921 caagttccac ttccggaagg tcgatacctt cacgaagaag atttatcccg ataagcacat
     4981 caaaagtgcc gtctctcaac tgtttcaata tctctattct ttccagtgtt tccacatcgg
     5041 aatgcaaata ctgcaccgat acacccaaag aagaatagta ctcggtcagt ttttccgcca
     5101 ttctttttgt taaggttgta accaatacac gatttttcct tgacacggta tcgcgtatct
     5161 cgaaaagcaa atcatccacc tgtcctttta cggggcgtac ctctatcttc ggatcggtaa
     5221 gtcccgtagg tcgtataatc tgctcggcta cctgctccga tatccccagc tcgtaatcgc
     5281 cgggagttgc cgacacataa atacattggt tctgtttttc cttaaactcc tcaaaagtca
     5341 gcggtctgtt gtcatatgca gaaggcagcc ggaatccgaa gtcaatcagg ttcttcttcc
     5401 tggaccgatc gcctttgttc atacctccta tctgaggaat tgttacatgc gactcatcta
     5461 tgaacattaa aaaatcctcg gggaagaagg aaagcaaagt ggccggcggc tgtccttcct
     5521 tcctgccgtc aatatgcctt gaataattct ctattcccga acagaaccct atattcctaa
     5581 tcatctccaa atcataactg gttctctgat tgagcctgta agcctccact atcttgcctt
     5641 gattttcaaa atatgccacc ctctcttgca gttccgcctg tatatcttct atggctcttt
     5701 taagttttgt atctttagaa acaaaatgac tggcgggaaa aaccgccaga tgattgacat
     5761 aacctgttat ttctccggtc aatgaatcaa actgcgtaat tttctccact tcatctccga
     5821 aaaagcttat tctgtacccg acatttgcac tgttggaagg agcaatttct aaagaatctc
     5881 cttttgccct gaaagttcct cttttaaaat ccatttcatt tcgtgagtac tgtatttcca
     5941 ccagtttctt tatcacatca tccctgtcaa gcgtcatgcc caccccgata tacagcatta
     6001 agtcctcata atcggcagga ctgcccagac cgtaaatgca ggaaaccgat gctactatta
     6061 ttacatccct tctttgcaga agagccgatg tggcggcatg tcgcagctga tctatttcgt
     6121 catttatgga catatccttt tcaatatatg tatcggtaga gggaatatat gcttcgggct
     6181 gataatagtc gtaataggat acaaagtatt ccacactgtt atcgggaaag aactctttaa
     6241 actccgaaca cagctgggca gccaatgtct tgttatgtgc aatgacaaga gtgggcttct
     6301 gaactttttc aataatattt gccatggtaa aagtcttacc cgaaccggta actcccagaa
     6361 gagtctgatg tttataccct gaaagcactc cgtttgccaa tgcctctatt gctgcaggct
     6421 ggtcacctgt aggtcggaaa tccgattgca aaacaaattt atctgatctg aactctttgt
     6481 ctctcctgct catgtaatta ttttagcaca caacacataa aaaattgggt taggttttct
     6541 aacatttagt gttgtatttt caaacagcgc gtattataat aatactgttg gtaaaggagg
     6601 agcaaaaatg aggggaatta atgagagaat taaaaaactg agaacacaga tgaatctgtc
     6661 ccaggactat gtagcaaaat atcttggtgt gagcaggtct actttcaccc agatggaaaa
     6721 tggcaacaga aaaatacttg ctgacgaaat ctcaaaactg agcacattat ttggcgtgtc
     6781 agtcaacgca atactggacg aaagtgagct gagccaaccg gccacagttt ttgccagaag
     6841 ttttgagaaa ctcgatgaat ccgatcaagc tgaaatcatg aaccttatcc gctttaagga
     6901 gcaaatgaag gcacagagga ctaaatgata cgatttgaaa tggtgaaaat agtaggctgc
     6961 atttttttat agctttttgt cattatgctg tataatggta taagtaaaat tgtgaacgca
     7021 aggagaacta tggaaaacaa aagaaaaccg gtattctgga tatttataaa gagctttacg
     7081 gacatgcatg catataaaca cttcgtgtat aagaattttt caaaagcatt cttatacatt
     7141 ctgctgttta cttttctgct ttccgttatt ttctcggcta tatttgtagc atcaattaaa
     7201 gattacacta cccaacttat aacgcaactg gaacaaaata ctccggaatt taccatcaaa
     7261 aacggagagc ttgtaataga gagtgatcaa ccggtgattc ttgcggaaac cgatgataaa
     7321 ggaacgctta taataatcga taaagaatat acgggatcgg ttaccaagta cgaagaatat
     7381 gatactgtta tagttttagg acaaaaggaa atatatttat ccaattcggc tataaggtac
     7441 agtataggtt accaatcggt tttaggtcaa aaacaggaag ttacttcgga aatgttgttc
     7501 ggcagagtat cttcatcaaa accctacctt tatattttct tctttgtatc tatgtttgtt
     7561 ttctttatcg gtaactacct cttctatgat ttgttcatag cattgatagt taatacaata
     7621 agacgtatca gaaaaagacc tgcaaatctg ggtatttcct tgcagcttac cgcttatgct
     7681 ctgacattac ctgctattat ctatcttatc tggattttca cttcactcat aactatcccc
     7741 atagaacctc ttatattcat tcttgcggtg attatagctt ataagggtgt agatgcctat
     7801 tacaagagta tagatgaaga gctgaccaag cccaaagaag aagcataatt tttattgaca
     7861 aacacttata cttactatac aatacaaaag cttgatttat cttatgacag aagggtggtg
     7921 aaaaacggat ggcagaagta agacttaaag agaacgaaac actggacagc gcattaaagc
     7981 gttttaagag acagtgcgcc aaagccggca tcttagctga agtaagaaaa agagaacact
     8041 acgaaaaacc cagcgtcaag aggaagaaga aatccgaagc cgcaagaaag agaaagaaaa
     8101 gatagtaatc aaacgggagc aaaagctccc gttgtatttt aattttttta taattatttc
     8161 ttaactattt gatttaaata ttttttatta atttaaacta ttacatccgt tatctaagga
     8221 tacaaatgct tggtaaaata ctctttacac caacccttca cccatatcct gaccgcccag
     8281 tacatgtatg tgtatgtgtt ttaccgtctg atgagcgtct tttcccgtgt tgttgataat
     8341 tcttactccg cttttttcta ttcccagtat ttcacttatc ttctttgccg cataaaacat
     8401 tctgccgatt atgaagcagt tgtcttcggt tatatcatta atatccgaca gtatatgctg
     8461 cttgggaata accaaaacat ggtgctttgc ctgaggattt atgtctttaa atgcatagac
     8521 ataatcatct tcgtatacct tggtacttgg tatctctttt cttacaattt tgcaaaaaat
     8581 acagttttcc attatattgc ctccttaata agttgtactg ctttttgaac ggcatcgtga
     8641 gccttgtcgg aatcttttcc gcctgcctga gccatatccg gtctgcctcc gcccgaaccg
     8701 ccgatcagtg ctgccgcttc tttaataacg gtaccggcat ttaccccgct tgtcaccgcc
     8761 tttttggttg cggctattac catcgatact ttctcgcctg ttgatacaaa tacgcaaaca
     8821 cagtcatcat atctttcacg tgctttgtcc gacaggtttc ttaaaacctg catatcggca
     8881 ttttcaataa cggcggaaac tacctttata ttcttatact gctcgaatgt gttaagaaca
     8941 ttgtccagtt tgtgagctga tagcttattc ttcaactcct cgttttcttt tcttaactct
     9001 ctgacggtgg aagaaagatt ttccactctg cctataacat cgcttccttt aagcatcctg
     9061 ttaagctgat taaccgtatc ttccagcttt tgcatatact caattgcatt tgtacctgtc
     9121 aatgcttcta tccttcgtac accggaagct accgcacctt ccgatactat tgtaaacaga
     9181 cctgtttctc ccgtttttga aagatgcgta cctccgcaca gttccatact ataaggatga
     9241 atggatacca cacgtacttt atcggagtat ttttctccga ataatgccgt ggcacctttc
     9301 ttcttagccg tatccatatc cattatttct ttcaccacat taatatcttc catgataatg
     9361 ttgttcacta tactcacaac tttcataagc tgccggtcat ccatagcgga aaaatgagta
     9421 aagtcaaatc tcagtctctg atgatctaca taggaaccgg cctgatgaac atgattaccc
     9481 aatacttctc ttaatgcgta gtgaagcata tgagtagccg tatggtttcg ttctgtagct
     9541 tttcttcttg atttatcaac tttaagagtc actgtttcat catcactgaa actgccttgc
     9601 tccatcacac ctctgtgaat gaagatgcct tctctgttta tcacatcggt aaccgtcatc
     9661 tttgcttttt ttccggttat aaccccgata tctgcctgct gaccgccgcc ttcggcatag
     9721 aataccgttt tgtcggttac cagttccgcc gtttcatctt tctttacgaa tgataaaact
     9781 tcagctttta tttcaagctt ttcataacct gaaaattccg ttttaggtat attcttgtat
     9841 atattctcat cactgtgcca tgcagaccct tcatctgctt taaaagcatc cctggcagtt
     9901 tttttctgat tttgcatata ctcgtcaaaa ccttccttat ccagtttgaa tcctctctcc
     9961 gaagcgattt ctctggtaag atcataggga aaaccgtatg tatcgtgaag tttgaaaatg
    10021 tgctcacctg caagagtatc tttcttgtta ttttcagcct cttcaaggta ctggtttaga
    10081 atgttcattc cgttttcaat ggtattaatg aatttggctt cttcactttg taccactttt
    10141 aatatatagt ctcttttttc ttccagttcc ggatatgcac tcttggaatt attaataaca
    10201 acatcaatta gttccgaaag aaaagcatct tttttaccta acagtcttcc gtgtctggat
    10261 gctcttctca acagtcttct taagacatag cctctgcctt cgtttgaagg tattataccg
    10321 tcggatatca tcataacgga acttcttata tggtcggtta tgacgcggat ggaaatatcc
    10381 tttgtctggt cttttccgta ttcagtgctg gtagcattgc agacaaaatc caatatgtct
    10441 ttaacggtat ccacttcaaa tatcgaatcc actccctgta ccacacaggc caatcgctcc
    10501 agtcccatac cggtatcaat gtttttattc tttaaaggtt tgtaagtgcc gtcttcctgc
    10561 ttgtcgaatt gtgtaaatac gttattccat acctctacaa atctgtcaca gtcacagcct
    10621 accttacagt cttttgaacc gcacccttta tcttctcctc tgtcatagta aatttccgag
    10681 cacggtccgc aaggtcctgt tccgtgctcc cagaaattat cttctttacc catccttaca
    10741 atatggctct catccagttt cacaatattt ttccagatat catatgcttc gtcgtcttct
    10801 tcgtagaccg atgcatagag aagattctta tccattttca gaacttcggt aaaaaactcc
    10861 cacgaccatt gaattgcctc tttcttaaag taatcgccga aagagaaatt acccagcatc
    10921 tcaaaaaatg ttccgtgtct tgtggtaatt cccacacggt cgatatccaa agttcttatg
    10981 cacttctggc aggtagttat ccttttagac ggcggtttta aagccccggt aaagtacggt
    11041 tttagcggtg tcattcccgc attaataagc agtatggaag gatcatcctc cggtatcaga
    11101 gaaaaactct tcaacctgag atgcccttta ctttcaaaaa atttcaggaa ctcttctctc
    11161 aatgtgttta atgataattt ctccatttcc atcaccatcc ttatataaat aaataactcc
    11221 catcctgcaa agggacgaga gttaacacgc ggtaccaccc aaattctttc ctaagaaagc
    11281 tcttaaacct ttaacgcagg tatacgttcc gttttgcgga aggcatcaaa gggcagcttt
    11341 cacatatatt cttgcaaggg ttctcaccgt tcccctctct ctgtgccaca gaccatatgt
    11401 tactcatcct tttagtaacg ccgtattaag gaaattatat cattttttca atgtatgtca
    11461 atatagctaa tccttgagtt tccgtacttt ttcacctacc gatttaaaca tctgtgtttt
    11521 ccattg
//
LOCUS       DAOSOM010000052        11394 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_5673_length_11394_cov_5.939589, whole genome shotgun sequence.
ACCESSION   DAOSOM010000052 DAOSOM010000000
VERSION     DAOSOM010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 11394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11394
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5673_length_11394_cov_5.939589"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="PLF14_08960"
     CDS             <1..772
                     /locus_tag="PLF14_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71451.1"
                     /translation="YMGNKFDKSVLMSGSRVCIVPGRMKGLGDCIYIKTEKVEEPIEY
                     LVIGTYDETEDIFFCPYLNYSELLTSSVNNIYSLKCEITDTKALEELETFLMNYFTKP
                     TIAGITPRGLNALDLKYTHSFVMPKGNFEEATDPIKADILKLKILEPFVFVFSVAIGF
                     VISMLSIRNRKAEFAVMRSMGASGGYVFYISFIEQAVLCLIGALIGVLVSLIGFKQLS
                     ATQINKVLIFIGCYLAGTAIEALRIASVKVMKIMKAND"
     gene            786..1460
                     /locus_tag="PLF14_08965"
     CDS             786..1460
                     /locus_tag="PLF14_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT71452.1"
                     /translation="MTNTPILQLEKVSYEYRNKYQTVRAVKDFSYEFFKGKFYAITGK
                     SGSGKTTLLSLMVGLDVPAKGVIKYDGINLKEMNMDKYRRDCISLIYQDFNLFPLLTA
                     MENVMYPLKLKGLKTSECETLAKEKLNMVGMDETFYKRYPSMLSGGEQQRVSIARALA
                     TGSSILLADEPTGNLDTENGDIIVSILKNLAHNNDFCVIVVTHDLEIASVTDVQLNLK
                     DGMLVT"
     gene            complement(1689..2660)
                     /locus_tag="PLF14_08970"
     CDS             complement(1689..2660)
                     /locus_tag="PLF14_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017690585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 family protein"
                     /protein_id="HOT71453.1"
                     /translation="MNNTWENKLELFAKNVQIMKSEFVWQNVSIKRMAALLYALEGKT
                     VDLEAIRQCHNLIKQNTGPFSTFRGSMALGLAAMLSLSGNPENSLSATLKVYKLLKDV
                     KFRSSYLLVVAAYRIAIQADASDYENAVTRTRAFFDGMKAKNLFYTGQDDYIFAAMSG
                     LSGLDLAEGTERIKQIYNQLKREFRNKNSVQILAQVLVLGEADDSIVNRVLALRDALR
                     TQKIKLDKAYTLPFLGIFALLPVEIEIIVNKIGEAQDALRNKKGFRTLSVSTGELLLL
                     AATIVASDYAENIKDGVLTATLSTCIANIIIAQEAAMVAAASSSSSV"
     gene            3015..3512
                     /locus_tag="PLF14_08975"
     CDS             3015..3512
                     /locus_tag="PLF14_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOT71454.1"
                     /translation="MSYSIGDKIVYPMHGAGVIVDKVNRKIMDEIRCYYVVKSPISEM
                     VAMLPVDNMDGIGIRDVITDSEADELLDEIKTMDTEETSNWNKRYRENMEKLKDGSIY
                     EIAQIAKSLMTRDKSLSTGERKLMINAKNAVLSEIMLAKNISLIQAEDALKKSIYCDS
                     DVEEE"
     gene            3516..4223
                     /gene="ispD"
                     /locus_tag="PLF14_08980"
     CDS             3516..4223
                     /gene="ispD"
                     /locus_tag="PLF14_08980"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:TIGR00453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOT71455.1"
                     /translation="MESVSVVIAAGGKSGRMDGINKLLAPLGGVPVIIRTVKAFHDIR
                     YIKQIILVIPEKDESEYLSLLSEYRVNRKVKIAYAGSTRRHSVYNGLLSLSDKEGIVL
                     IHDGARPLVTRKIIDECVDGAKRYGVCVAASKSTDTVKIADSIMFIEKTLDRDKVYNA
                     QTPQAFKTEFALDFHKRAEADDLEVTDDCMIAEHYGHNVKIIESSPLNLKITTKLDLV
                     IASAILADGNNTETHDS"
     gene            complement(4185..4646)
                     /gene="ispF"
                     /locus_tag="PLF14_08985"
     CDS             complement(4185..4646)
                     /gene="ispF"
                     /locus_tag="PLF14_08985"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOT71456.1"
                     /translation="MKCAIGQDSHKFSENADKKLILAGVHIKNHPGLIANSDGDVIYH
                     ALTNAISGITSVNILGKTADILCKGGITDSSLYVKEALKSLEYKIEHVSFTVECMTPV
                     LSPYIEEMKGNIAMLLGIDKKDVGLTATSGEGLSSFGKGSGIMCFCIITVS"
     gene            4773..5270
                     /locus_tag="PLF14_08990"
     CDS             4773..5270
                     /locus_tag="PLF14_08990"
                     /inference="COORDINATES: protein motif:HMM:NF040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6106 family protein"
                     /protein_id="HOT71457.1"
                     /translation="MESWYYEKIVYRKKTKKDDLISFAVVLVTLAICIVSYYFLRNLS
                     FIIWLGVIYACYRIITSRKFDYEYIFVEDTMTIDKIINKSRRKNIFTHSLKDFEILAY
                     KDSDHIKEYSDNVQQVIDASAPGSENTLFGVAYFKSMRTLVYFEADERIEEFIQKYAK
                     HKFKE"
     gene            5274..7004
                     /locus_tag="PLF14_08995"
     CDS             5274..7004
                     /locus_tag="PLF14_08995"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HOT71458.1"
                     /translation="MKKLWKYVILIIILAALAAAVFFAYDAVLNNVREKYSPNTNLEK
                     YFSDEYTDLVVNFHKVEYEKPVRIINGEILVRYEVIKQFIDPYIYYDETRYKVIITTK
                     DRMVNMTEDSLVAKLNKKDLHLDTASQRHDGYLYVPVSPFLYMWNISVKYLEQNDIVI
                     IEFLRNFDHTATVNIADAQLRKGPSIKEPVYVSNVPVGQVLYVNRVLGDWTYLMTSEG
                     VAGYIETKYLDIKVTSKQPVVVRYEQKKLYLPEYVVLSWHYVHSSSVTYVPYYADVNV
                     LSPTFFTVIDEKGNIASSASVKYMENARKRGYTVWPLINNVFAKKGQISEVLADSEAR
                     RHVIDQILTYAYIYKFDGINIDFENLYLSDKDNLTQFMRELVPLAHQMNLAVSIDVGV
                     PGGSEAYSRCYDHEELGKVADYVMVMTYDQYWSAHETGGSQAQLSWVEESVGKTLKLI
                     EPDKLVLGIPAYTRLWKTDSEGKVTPDKTLTADQVVKLVEEKQLTPVWEEERDGYISG
                     QYYIEYIEDGELYRAWIEDDASAREKAALAAKYNLRGVCIWMMSQTNKTVWEAILEGF
                     NADVNQVISD"
     gene            7081..7572
                     /locus_tag="PLF14_09000"
     CDS             7081..7572
                     /locus_tag="PLF14_09000"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOT71459.1"
                     /translation="MQIKVNYEDIFERLNGQLPKGVFLNTDFEGKYNTMTIGWALMGI
                     CWGQPVVCVAVRFSRYTYELLEQSGKFTLSIPKSGAMTKELSLCGSRSGRDVDKFELC
                     GFKKSNIIDKCDLHIECEVVQKTAMNKDFIEKSIKSRYYRTDDYHMFYFGKITDCYYT
                     KEG"
     gene            7576..8649
                     /locus_tag="PLF14_09005"
     CDS             7576..8649
                     /locus_tag="PLF14_09005"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HOT71460.1"
                     /translation="MKNVCVIFGGASTEHEVSRVSASFVMKNLDKKKYSVTVMEMTKN
                     REYLLYEYREGETFFEEIVKVDNNLPRKRIIPDISMFERMGVDVVFPVIHGTMGEDGK
                     LQGFLELCDIPYVGCDVLSSAVCMDKVLCKDILLKNGIAVADYLWFTCDEIGKDIGNV
                     VKAVESKFVYPIFVKPSECGSSVGITKAKDTESLKKALVFAARFSRKVLVEEFVEGLE
                     LEVAVLGSYDKVSSSGVGQIISSNEFYDYNAKYIDGKSLTVLNPDIPEWSEREIRKIA
                     EKAFCVIGCYGLARVDFFLTKGKKAVLNEINTIPGFTSISMYPMLWGQADISYDKLLT
                     KLITLAQDRKKRYKFQTDYKGGE"
     gene            8649..9458
                     /gene="murI"
                     /locus_tag="PLF14_09010"
     CDS             8649..9458
                     /gene="murI"
                     /locus_tag="PLF14_09010"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOT71461.1"
                     /translation="MTEGKIGVFDSGIGGLTVLKEILRCLPYEDIIYFGDGKRTPYGG
                     KSKETIELFALQSMKFLTDKGAKAVVIACNTVSSNAMDAIRAHYSLPVVEVISTTAKL
                     AFSQSKNKKIGVIGTKATIESGSYEKALKSMDASVSVYQKSCPLFVPLAEEGAEWWDD
                     EITEAVARRYFGYFDNTGIDTLILGCTHYPLLKKVIARTIGEHVNLVDSALITAKQTE
                     KLLEESNLLKEGKKKGALEVYTSDSILRFMPMCSAILGINNLNIQKCYLGE"
     gene            9463..>11394
                     /locus_tag="PLF14_09015"
     CDS             9463..>11394
                     /locus_tag="PLF14_09015"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOT71462.1"
                     /translation="MKDRLRFRQVHLDFHTGEQVTGIGAKFNPEEFAKTLKDAHVNSV
                     TCFARCHHGMIYYDSKINKERIHPHLVEKDLLKKQIEACHKYDIKVPVYTTVQWDLYT
                     AKKHPEWLVRTPEGSVDGNYFNKDGFYQRICVNTPYMDFLKKHVTELLTELDCDGVFL
                     DIVGDLDCSCVYCMESMEKGGFNPLDENDRRAHYRQVMTNFKKEMSRLIWSLKPGILI
                     FYNGGHVSYKDKLPSKDTYSHYEIESLPSGGWGYMHFPVTCRYARNLGKDYLAHTGKF
                     HTSWGDFHSFKNIEALEYEVFRMLALNSKCLIGDQLEPDGALSKPVYDLIGKVYSRVE
                     EKEPWCKNADHVAEIGVFVPTDEKTRVSASQAAVESLLDQLAYQFDFIDPYMDFSRYR
                     MLIIPERITIDEQTDKKLCGYLKNGGKVLSVFDAIFSGSGVKVIKETLDDYGQPVKGR
                     FVARNGAADYVIPTDLIGKELYKTEYVMYAKGMETVATDGKALVKAYEPIFNRSFRHF
                     SSHAQAPSSHIEAYDAVVINRSKNAIYFSHPIFEIYNKKGPKWIKSMLHDAIKLLLKD
                     NLISHDGPSTLFTGMNVQKDKKRYILHLLHYIPERRCTDFDLIEDVIPLYRIKINLNL
                     PKTIKSITVVPENKPVKFTG"
BASE COUNT         3613 a         1994 c         2570 g         3217 t
ORIGIN      
        1 atatatgggg aataagtttg ataaaagtgt actaatgagc ggcagccgcg tatgtattgt
       61 gcccggcaga atgaaaggtc tgggcgactg tatatacatc aaaacggaaa aagttgaaga
      121 gcctatagaa tatctggtga taggaacata tgatgagact gaagacatat tcttttgccc
      181 ttatcttaat tacagcgaat tgttaacttc ctccgtcaat aacatataca gtcttaaatg
      241 tgaaattacc gacacaaaag cattagagga actggaaaca tttttaatga actacttcac
      301 caagcctaca atagcaggta ttacgccaag aggtttaaat gcattagacc tgaaatatac
      361 tcattctttt gtaatgccca aaggcaattt tgaagaggcg acagatccca taaaagcgga
      421 cattttgaaa cttaagatat tggagccgtt tgtattcgtg ttttccgttg ccataggttt
      481 tgtcataagt atgctgtcta taagaaacag aaaagcggaa tttgccgtta tgagaagtat
      541 gggagcttcg ggaggctatg ttttctatat cagtttcatt gaacaggccg tattatgttt
      601 aataggtgca ttgataggag ttttagtatc gcttatagga tttaagcaat taagtgcgac
      661 acagattaat aaggtattga tttttatcgg ctgttatctt gcgggtacgg cgatagaagc
      721 attaagaata gcaagtgtta aagtaatgaa aataatgaaa gcgaacgatt aaggagaccg
      781 acaaaatgac aaatacaccg atattgcagt tggaaaaagt aagctatgaa tacagaaaca
      841 aatatcagac ggtaagagcg gtaaaggatt tttcttatga attctttaag gggaaattct
      901 acgcaataac aggaaaatcc ggttcgggga aaaccacttt attatcatta atggtagggt
      961 tggatgttcc cgcaaaaggc gttattaaat acgacgggat caatcttaaa gagatgaata
     1021 tggataaata cagaagagat tgtatttcac tgatatatca ggatttcaat ctgtttccgc
     1081 tgcttaccgc gatggagaat gtaatgtatc ctttaaaact aaaaggattg aaaacaagcg
     1141 agtgcgagac acttgcaaag gaaaagctga atatggtagg gatggatgaa actttttata
     1201 aacgctatcc ttccatgttg tcgggaggcg aacagcagag agtatcgatt gcacgtgctt
     1261 tggcaaccgg ctcctcaata cttttagcgg atgagccgac gggtaacctt gatacggaga
     1321 atggcgatat aattgtttcc atattaaaga atcttgcaca caacaacgat ttctgtgtta
     1381 tagttgtaac acatgatttg gaaattgctt cggttaccga tgtgcagctg aacttaaagg
     1441 acggtatgct tgttacttag atactgttga tttttccact gatatttact tcaatttact
     1501 attcacggtt ttccgaactc ggtcgaaaag ctttttgcaa ccgttgcata atcttgtgtt
     1561 tcctttgagt cttcgaatac atattgttcc aagaaaggat tgtcataatt cttttattat
     1621 tgttatatcc tgtttcatgc gtaatctgat tgctatttaa tcacgaacgc ttcttattca
     1681 gcatattgtt aaactgatga agatgacgat gcagctgcta ccatagcagc ttcctgcgct
     1741 ataataatat tggctataca tgttgaaagc gttgccgtca gcactccgtc ttttatgttt
     1801 tcggcatagt cgcttgctac aattgttgcc gctaacagaa gcaactcccc cgtacttacg
     1861 gacagagttc tgaatccctt tttgttccta agagcatcct gtgcttctcc tattttatta
     1921 acgattattt caatttctac cggcagcagt gcaaagattc ccaaaaacgg taatgtataa
     1981 gctttgtcca gtttgatttt ttgcgtcctg agcgcatcac gcagagcaag aacacgatta
     2041 acaatactgt cgtcagcctc tccgagcacc agcacctgcg ccagaatctg aacgctgttt
     2101 ttattcctga actctctttt aagctgatta taaatttgct tgatgcgctc cgttccttcg
     2161 gcaagatcca aaccggaaag ccccgacata gccgcaaaaa tatagtcatc ctgcccggtg
     2221 taaaataaat ttttagcttt cattccgtca aaaaatgccc tcgtgcgtgt taccgcgttt
     2281 tcataatcgg aagcatcggc ttgtattgca atccggtatg cggcaaccac caaaagatag
     2341 gaagatctga actttacgtc tttcagcaac ttgtatactt taagggttgc acttagcgaa
     2401 ttctcaggat taccggatag agagagcatt gccgccaaac ccagcgccat gcttccgcgg
     2461 aatgtggaaa acgggcccgt gttctgttta atcaaattgt gacactgcct gattgcctct
     2521 aagtcaaccg ttttgccctc cagtgcatac agaagcgccg ccatacgctt tatcgagaca
     2581 ttttgccaga caaactccga tttcataatt tgcacgtttt ttgcaaaaag ttccaacttg
     2641 ttttcccatg tgttattcat ctgaaaagac ctccgtaaac tgaatttgct attatgtaaa
     2701 tattaaataa ggttgtgtct aatcatcttt cctgtatttg tgtcatattt ataatcaact
     2761 taattataca ctcagctttc tcttagctca atattccgca ccgcttaaat atttttgaca
     2821 ctgctgctat tcacggcgca cgtgctttaa tgcggaaaat ggcttaaaag aaaagtttta
     2881 aagaggcaac attcgttcat tttttatgtt ttgtcaaaaa aaatcatgtt caattttcct
     2941 gctcattttc gcctgtgttg cttttgacag caggcactaa aaagagtata atatcgggta
     3001 ggaggtatct ttaattgagt tacagtatcg gggataagat agtatatcct atgcatggcg
     3061 caggcgtcat tgttgataaa gtcaacagaa agattatgga tgaaatccga tgctattatg
     3121 tagtaaaaag tcccataagc gaaatggtcg ctatgcttcc cgtggataat atggacggta
     3181 taggtattag ggatgttatc accgattccg aagcggatga acttctggat gagataaaga
     3241 caatggatac cgaagaaaca tccaactgga ataagagata ccgtgaaaac atggagaaac
     3301 tcaaagacgg atcaatatat gaaatagctc agattgctaa atctttaatg acccgtgaca
     3361 aaagcctctc gacaggtgag agaaaattga tgattaatgc gaaaaatgcc gtattgagtg
     3421 aaataatgtt ggctaagaat atttctctga ttcaggcgga agacgcttta aaaaaaagta
     3481 tatactgcga ttcggatgtt gaggaagaat aatatatgga atcggtaagt gtggttattg
     3541 ctgcgggcgg taaatccggc aggatggacg gaataaacaa actgttggca ccccttggcg
     3601 gtgttcccgt tattatccgg acggttaaag cgtttcatga tataagatac attaaacaga
     3661 taatacttgt tatacctgaa aaagatgagt cggagtactt atctttattg tctgaatata
     3721 gggtgaatag aaaagtaaag attgcgtatg ccggaagcac ccgcagacat tctgtgtata
     3781 acgggttgct gtcattatct gacaaagaag gtattgtact tatacacgac ggtgccagac
     3841 cgctggttac ccgtaagatt atcgatgagt gtgtagacgg agcaaagcga tacggagtat
     3901 gtgtggccgc ttcaaagagc accgatacag taaagatagc ggacagcata atgtttatag
     3961 aaaaaaccct tgacagggac aaggtataca atgcccagac accgcaggct ttcaagacgg
     4021 aatttgcttt ggattttcac aaaagagcgg aagcggatga tctggaagtc accgatgact
     4081 gcatgatagc ggagcattac ggacataatg taaagattat agagtcgagt cctttgaatt
     4141 taaaaataac taccaaattg gatttggtaa tagcttctgc tattttagct gacggtaata
     4201 atacagaaac acatgattcc tgaacctttt ccgaatgatg acaaaccttc acccgatgtg
     4261 gccgtcaaac ccacatcttt tttatctatt ccgagaagca ttgctatatt gcctttcatt
     4321 tcctctatgt aaggagagag aaccggagtc atacactcca ccgtaaagct gacatgctct
     4381 attttatatt ccaggctttt caaagcttct tttacatata gagaactgtc ggttattcct
     4441 cctttgcaca ggatatctgc ggtttttcct aaaatgttta ccgaggtaat accgcttatc
     4501 gcattggtca gagcatgata gataacatct ccgtcgctgt ttgcgataag tcccggatga
     4561 ttttttatgt gaactcccgc aagaataagc ttcttgtctg cattttcgga aaacttatga
     4621 ctgtcctgcc cgattgcgca cttcatgatg tctgtcacct cgttgtttaa tacctgaata
     4681 acataatacc ataatatcgg aaaagttaac aaaaagtaca cacataaaaa ccctttatat
     4741 gatatattta gcatattgaa aggaagatgc atatggaaag ttggtattat gagaagattg
     4801 tatacagaaa gaaaacaaaa aaagatgatt tgatttcatt tgcagttgtt ttagttacgc
     4861 ttgcgatatg tatagtttcc tattactttc tgcgaaatct ctcatttatt atctggttgg
     4921 gtgtcatata tgcatgctac aggattataa cttccaggaa attcgactat gaatatattt
     4981 tcgtggaaga taccatgacc atcgacaaga ttataaataa aagcagaagg aagaatatat
     5041 ttactcattc gttgaaagat ttcgagatac tggcatataa ggattccgat catataaagg
     5101 agtattcgga taatgtacag caggtaatag atgcctccgc accgggttcg gaaaataccc
     5161 ttttcggagt ggcatatttc aaatccatga ggacacttgt gtacttcgag gcagatgaaa
     5221 gaatagaaga gtttattcaa aaatatgcca agcataaatt caaggagtaa ttattgaaga
     5281 aactgtggaa atatgtcatc ttaataatta ttttagcggc attggcggca gcggtctttt
     5341 ttgcgtatga cgccgtactg aataatgtgc gggaaaagta ttctccgaat actaatcttg
     5401 aaaagtattt ttccgatgag tataccgatc tggttgtcaa tttccataaa gttgaatatg
     5461 aaaagcccgt cagaattata aacggcgaga tactggtgcg atacgaggtt ataaagcagt
     5521 tcatcgatcc gtacatatat tacgatgaaa ccagatataa agtgattatt accacaaaag
     5581 acagaatggt gaatatgaca gaggacagtc ttgttgcaaa attgaataaa aaggatcttc
     5641 atctggatac cgcaagtcaa agacatgacg gatatcttta tgtaccggtt agcccttttt
     5701 tgtatatgtg gaacatcagt gtcaagtatc tggagcaaaa cgacatagtg ataatagagt
     5761 tcttaaggaa tttcgaccac acggcaacgg tcaatattgc cgatgctcag ctgagaaaag
     5821 gaccttccat aaaagaacct gtttatgtat caaatgtacc tgtgggtcag gttttgtatg
     5881 tcaacagggt tttaggcgac tggacttatc ttatgacaag cgaaggtgtt gcgggataca
     5941 tagaaacaaa atacctggat attaaagtta cctctaaaca gccggttgtt gtcaggtatg
     6001 agcaaaaaaa gctgtatctg ccggagtatg tggtgctttc ctggcattat gtacattcca
     6061 gttcggtaac gtatgtgccg tattatgccg atgtcaatgt cctgtctccc acatttttta
     6121 cggttataga tgaaaaaggc aatattgcca gcagtgcatc cgtgaagtat atggaaaatg
     6181 cgagaaaaag agggtatacc gtctggccgt tgattaataa tgtttttgca aagaaagggc
     6241 agataagtga agtattggct gacagtgagg caaggcgtca tgtgatagac cagattctga
     6301 cttatgcata tatatataaa ttcgacggga taaatataga ttttgaaaat ctgtatctgt
     6361 ccgataagga taatctcaca cagtttatgc gggagctggt tcctttggca catcagatga
     6421 atctggccgt ttccatagat gtgggagttc cgggaggatc ggaggcttat tcacggtgtt
     6481 atgatcacga agaactggga aaagtagccg attatgtaat ggtaatgaca tatgaccaat
     6541 actggagtgc acatgagacc ggcggttcgc aggctcagct ttcctgggtg gaagaaagtg
     6601 tcggcaagac tttaaaactg attgagccgg ataaactggt tttaggtata cccgcatata
     6661 ccagattgtg gaaaaccgac agtgaaggaa aagtgactcc ggataagaca ctgacagccg
     6721 atcaggtggt aaaacttgtt gaggaaaagc agttaactcc ggtgtgggaa gaagagagag
     6781 acggatatat aagcggacag tactatattg aatatataga agacggggaa ctgtacaggg
     6841 catggataga agatgatgcc agtgcaagag aaaaagccgc acttgccgcc aaatacaatt
     6901 taagaggtgt atgtatctgg atgatgagtc agaccaacaa aacggtatgg gaagccattc
     6961 ttgaaggttt caatgcggat gttaatcagg taatatctga ttaaacttat catgttttct
     7021 tatgtgcttt aatgtggata tggtatataa ttaattaaaa cgtcggaaag gaaaaactga
     7081 atgcaaatta aagtgaacta tgaagatatc tttgaaagat taaacggaca actgccgaaa
     7141 ggtgtttttc tcaataccga ttttgaaggc aaatacaata caatgaccat aggctgggca
     7201 ttgatgggaa tatgctgggg acaacctgtt gtatgtgttg cggtcagatt ttccagatac
     7261 acatatgagc ttttggagca gtcgggtaaa ttcactttaa gcattcccaa aagcggagca
     7321 atgacaaagg aactttcact ctgcggctca agatcgggac gcgatgtgga caagtttgaa
     7381 ttatgcggat ttaagaaaag caatattata gataagtgcg atcttcacat tgaatgtgaa
     7441 gtggtacaga aaaccgcaat gaacaaagac tttattgaga aatctattaa aagcagatat
     7501 tacagaacag atgattatca tatgttctat ttcggaaaaa taaccgactg ctattatact
     7561 aaagagggtt gaaatatgaa aaatgtatgt gtaattttcg gaggtgcttc cacggaacat
     7621 gaggtttccc gcgtatccgc ctcatttgtt atgaaaaacc tggataagaa aaagtattcg
     7681 gttaccgtaa tggaaatgac taaaaacagg gaatatttgc tgtatgagta tagggaaggt
     7741 gaaactttct ttgaagagat agtcaaggtg gataacaatc ttcccaggaa acgtattatt
     7801 ccggatattt ccatgtttga gaggatgggt gtggatgttg tttttcccgt tatccacgga
     7861 acaatggggg aagacgggaa attgcagggt ttcttagagt tatgcgatat tccctatgta
     7921 ggctgcgatg ttctttcgtc ggctgtgtgc atggataaag tgctttgcaa agatatattg
     7981 ttgaaaaacg gtatagccgt tgcggactat ttatggttta cctgtgatga aataggtaaa
     8041 gatataggta atgttgtaaa agccgttgaa agtaaatttg tataccctat ctttgtaaaa
     8101 ccgtcggaat gcggatcttc ggtgggtata accaaggcga aagatacgga aagtctgaaa
     8161 aaagcccttg tatttgctgc acgcttttcc agaaaggttc ttgtggaaga atttgtggaa
     8221 ggtctggaac tggaagttgc ggtattgggc tcatatgaca aagtatcttc gtcgggcgtg
     8281 ggacagatta tttcctccaa tgagttttat gattacaatg caaaatatat agacggcaag
     8341 agccttacgg tattaaatcc cgatataccg gaatggtcgg agcgggagat aagaaaaatt
     8401 gccgaaaagg cattttgcgt tatagggtgt tacggattgg cgcgggtgga ctttttcctc
     8461 acaaaaggca aaaaagccgt tctcaatgaa ataaatacca ttcccggttt cacaagtata
     8521 agtatgtatc cgatgttgtg gggacaagcg gatatatcct acgacaagct tcttacgaaa
     8581 cttatcactc ttgcacaaga caggaaaaaa cgatacaaat ttcaaacgga ctataaaggc
     8641 ggggaataat gacggaaggt aaaatagggg tatttgattc gggaataggg gggcttacgg
     8701 tacttaaaga gattttgcgc tgccttcctt atgaagacat aatatatttc ggcgacggta
     8761 agagaactcc ttacggagga aaatccaaag agacaataga actgtttgct ttacagagca
     8821 tgaaattcct gaccgataaa ggtgccaaag cggtggtaat agcgtgtaat accgtaagtt
     8881 ccaacgctat ggatgcaata agagcacact acagtctgcc ggttgtggaa gttatatcca
     8941 ccacggcgaa actggctttt tcccaatcga agaataaaaa gataggggta ataggaacaa
     9001 aggccacaat agaaagcggt tcatatgaaa aagcattgaa atcaatggat gcatcggtat
     9061 cggtttatca gaaaagttgc ccgctgttcg tgccgcttgc ggaagaaggt gccgagtggt
     9121 gggacgatga aataaccgag gcggttgcaa gaagatattt cggttatttt gataacaccg
     9181 gtatagatac ccttatattg ggatgcacac attatccgct tctgaaaaaa gtaatagccc
     9241 gcacaatagg ggagcatgtt aatctggtgg attcagcatt gattacagcc aagcagactg
     9301 aaaaactgtt ggaagaaagc aacttgctga aagagggaaa gaaaaaaggt gctctggaag
     9361 tatacacttc cgacagtatt ttgaggttta tgcccatgtg ttcggcaatt ttaggtatta
     9421 acaacttaaa tatacaaaaa tgctatttag gagagtaaaa ctatgaaaga cagattacgt
     9481 ttcagacagg tacatttgga tttccatacg ggcgaacagg ttaccggtat aggtgcaaaa
     9541 tttaatccgg aagagttcgc aaaaacatta aaagacgctc atgtgaattc cgttacatgt
     9601 tttgcaagat gccatcacgg aatgatatat tacgattcca aaataaataa agaaagaata
     9661 catcctcatc tggtagagaa ggatttatta aaaaaacaga ttgaagcctg tcataagtat
     9721 gatataaagg ttcccgtgta cactaccgta caatgggatt tatacaccgc caaaaagcat
     9781 cccgaatggc tggtaagaac gcctgaagga tctgtcgacg gcaattactt caacaaggac
     9841 ggtttctatc agaggatttg cgtcaacaca ccgtatatgg attttttaaa gaagcatgtg
     9901 acggaactgt tgacggaact ggactgtgac ggagtgtttt tggatatagt gggagatctg
     9961 gactgctcct gcgtctactg tatggagagt atggaaaaag gcggttttaa ccctcttgac
    10021 gaaaacgaca gaagagcaca ttacagacag gttatgacaa atttcaaaaa ggaaatgtcc
    10081 cgacttattt ggtcattgaa acccggtata ttgatattct ataacggcgg ccatgtctcc
    10141 tataaagata agttgccgtc aaaagataca tactcacatt atgagataga gagtcttccg
    10201 agcgggggat ggggctatat gcactttccg gtaacctgca gatatgcgcg aaatttaggt
    10261 aaagactatc tggcacacac cggcaaattc catacaagct ggggcgactt tcactcattc
    10321 aagaatattg aggctttaga atatgaagtg ttcagaatgc tggctcttaa ttccaagtgt
    10381 ctgataggag atcagttgga acccgacgga gctttgtcaa aacctgtata tgatttaata
    10441 ggcaaggttt attcacgggt tgaagagaag gaaccgtggt gcaaaaatgc cgaccatgtg
    10501 gcggaaatag gggtgtttgt acccacggat gaaaaaacca gagtttccgc ctctcaggcc
    10561 gctgtagaga gtcttcttga ccagttggct tatcagtttg attttataga tccgtatatg
    10621 gattttagca gatatagaat gcttatcatt cccgagagaa taacaataga tgaacaaacg
    10681 gataagaagc tttgcgggta tctgaaaaac gggggcaaag tcttatcggt atttgatgcc
    10741 atattctccg gatcgggagt taaagtcatt aaagaaacat tagacgatta cgggcaaccg
    10801 gttaaaggaa gatttgttgc aagaaacgga gccgccgatt atgttatccc taccgatctg
    10861 attggtaagg agttgtataa aacggagtat gtaatgtatg caaaaggcat ggaaaccgtt
    10921 gcaactgacg gaaaggctct tgtcaaagct tacgagccca tattcaacag atcattcagg
    10981 catttctctt cacatgcaca ggccccttct tctcacattg aagcatacga tgcggtggtg
    11041 ataaacagga gtaaaaatgc gatatatttc agccatccca tatttgagat ttataacaaa
    11101 aaggggccca aatggataaa gagcatgctt catgatgcaa taaagctttt attaaaagac
    11161 aaccttatat ctcatgacgg accgtcaacg ctctttacgg gaatgaatgt acagaaagat
    11221 aagaaaaggt atattctgca cctgctccat tacattcccg aaagacgatg taccgacttt
    11281 gatctcatcg aagatgtgat acctttatac aggataaaga taaacttaaa ccttccgaag
    11341 acaattaaga gtattaccgt tgtgccggaa aacaagcctg ttaagtttac gggg
//
LOCUS       DAOSOM010000053        11226 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_5771_length_11226_cov_4.770298, whole genome shotgun sequence.
ACCESSION   DAOSOM010000053 DAOSOM010000000
VERSION     DAOSOM010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 11226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11226
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5771_length_11226_cov_4.770298"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1347)
                     /locus_tag="PLF14_09020"
     CDS             complement(<1..1347)
                     /locus_tag="PLF14_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOT71463.1"
                     /translation="MKLKFDDNLEYQLQAIASVVDLFSGQTPMHTNFTVSAYSGQIGL
                     FDTANGVGNRLELDKEEILSNLQNIQLRNRLPQTATLKAGMYDFDIEMETGTGKTYVY
                     IRTILELHKNYGFSKFIIVVPSVAIKEGVYKSLQVTKEHFQELYDNTIYNFFVYDSSN
                     LEQVRSFAVSSNIEIMVINIDAFRKSFKDPTKENKANIIHRAHDRLSGMRPIELIQET
                     RPFVIIDEPQSVDTTPKAKEAIRSLNPLCTLRYSATHVEKHNLVYKLDAVDSYNLELV
                     KQIEVAGFETKDYHNKAYIKLISVNNKKSPITAKMELDIKNRRGEVKRDTVTVKQGDD
                     LYEKSNGRDVYDGYIVNDIYCEKGNEYVDFTSKPDILFIGKPIGDVDDLVVKEQQIRK
                     TIEEHLNKELVLNVKQDRSKDEAWKIIVATVLGKFHVLEMNDEKSGYSEDKKIIIRI"
     gene            complement(1362..3221)
                     /locus_tag="PLF14_09025"
     CDS             complement(1362..3221)
                     /locus_tag="PLF14_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOT71464.1"
                     /translation="MKISTLDGKTPDIAEKNIQMLKQIFPEVFSEGKVDFDKLKQVLG
                     DYVEDSTERYNFTWNGKGQALRLSQSPSLGTLRPCKEESKDWDTTQNLYIEGDNLEVL
                     KLLQKSYHGKIKMIYIDPPYNTGEDFVYPDNFHDSIENYKKITGQVDDEGKKIGTNTE
                     TSGRYHTNWLNMMYPRLRLARNLLADDGVIFVSVDYNENFNCRTILNEIFGLSNFIGE
                     IYWESKTKSQNTETAYNKLQPKAEMILVYTKQLKRRFNLIVKELKDYPLSDEQGNYRE
                     YPLEVMNATGIRGRETMIYDVSDGISTVSPPPGKQWQLGQDQVAAYKDSGNLFIREGK
                     VIIKMRPEFERNETTEPFWGFFSKELGTAENAKSDLTELIGEHGFETVKPIDVIKRLI
                     FHATEKNDIILDFFSGSATTAHAVMQLNADDGDSGKRKFIMVQLPELTDKKSTTYKAG
                     YVNVSEIGKERIRRAGEKIKSEAGLFAQNLDVGFKVFKLDTSNIRKWQPDYDNLEVSL
                     LDYVDNFVEGRTELDVVYEIMLKYGFDLTYSVDEFIVSEKKVYSVGYGMLMICLSNGI
                     KPDVAEGILKRVKELSPESSRVVFKDNGFEKDSDKTNIKEILKVGGIKEFITI"
     gene            3498..4844
                     /locus_tag="PLF14_09030"
     CDS             3498..4844
                     /locus_tag="PLF14_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HOT71465.1"
                     /translation="MNRYYESESVELKAGYTDTVVREIVSFLNTEGGVIYIGIEDDGT
                     VIGIENVEGVLRQVSDCITDQIEPKPIGEVISAVILEDNLPIVVVNVKKGIKPIYCIK
                     KYGFSSKGCPVRIGNTCKEMSPEEIQYRYKSLFVDQDYMLLASAKYAPLSFEMMKILL
                     TGRGYHINESSFESSFKLRRNDGTYNLLAEVLADKNMVPLIFVKFSGPDKTSISHRSD
                     YGGQSILLGYQKMKDRLIAENICKTDTSVRPRIDEYLYDIDCVNEALVNMLVHNDWTV
                     TEPLVSFYSDRVVFTSHGGIPQGITEEEFYNGVSQPRNALLMRIFLNMGIVEHTGHGV
                     PMIVKRYGREVFDIHKGYIDVTIPFNKNVLASVPNSVINESAVGYGVKVYKDLTDTEK
                     EAILVLVEKPDITYDKLALEINVSRRTVARTIASLTEKKYIERVGNNKVGFWKVIR"
     gene            complement(4855..5349)
                     /locus_tag="PLF14_09035"
     CDS             complement(4855..5349)
                     /locus_tag="PLF14_09035"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT71466.1"
                     /translation="MEFIIRESVTDQDKHGKAYVHYTAWNETYTGLMPQEFLDGRSLE
                     KCIKNAYEYPKNTFVAVAKEKVVGFALYIPESRELVSIKPSSEVVAIYVLQKYQRNGI
                     GEALLKQCIKVLPRNPIIIFVLKGNEKAINFYKKQGFKFTGKTVEQNVKGGTLVELEM
                     VLER"
     gene            5505..5732
                     /locus_tag="PLF14_09040"
     CDS             5505..5732
                     /locus_tag="PLF14_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012704205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PC4/YdbC family ssDNA-binding protein"
                     /protein_id="HOT71467.1"
                     /translation="MAEISYKIEKEIGVLSEAPSGWTRQINLVSWNGREAKLEIRDWA
                     PNRERAGRGMTFTKDEVRKLVKFLIKAELDK"
     gene            5866..7254
                     /gene="asnS"
                     /locus_tag="PLF14_09045"
     CDS             5866..7254
                     /gene="asnS"
                     /locus_tag="PLF14_09045"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOT71468.1"
                     /translation="MKISVQRLYDSPNDYKDMIVTVEGWVRTIRSSKDFGFIELNDGT
                     CFKNIQIVFENNVVNNYAEVEKLPVASSISVTGKIILTPDAKQPFEIKAEDIEICGVS
                     ASDYPLQKKRHSFEYLRTVAHLRPRTNTFFAVFKVRSVLAQAIHTFFQDRGFVYVHTP
                     IITASDCEGAGEMFQVTTLDLDRIAGSKEKVDYSKDFFGKQTNLTVSGQLSVETYCMA
                     FRNVYTFGPTFRAENSNTPRHASEFWMIEPEMAFADLDDDMSMAEQMIKYIINYVMER
                     CPDEMEFFNKFIEPGLFDKLKVVTDSKFDHVTYTDAIKILKESKVLFENPVEWGCDLQ
                     TEHERYLTEKHFKKPVFVTDYPKAIKAFYMKVNDDNKTVRAMDLLVPQVGEIIGGSQR
                     EERHDVLVARMIEMGLAPEDYWWYLDTRKYGTVKHAGFGLGFERMIMYMTGMSNIRDV
                     IPFPRVPKHAEF"
     gene            7257..7562
                     /locus_tag="PLF14_09050"
     CDS             7257..7562
                     /locus_tag="PLF14_09050"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOT71469.1"
                     /translation="MINNSGTDIAESVIIVKIAQTVALFDTVRLTKKKKAVKLSDSGK
                     LKTVEIFLDINYNIVIPEVASKIQKAVAKVLEKELDIVMDSIDVTVVGVNIEKLGTE"
     gene            7559..8599
                     /locus_tag="PLF14_09055"
     CDS             7559..8599
                     /locus_tag="PLF14_09055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOT71470.1"
                     /translation="MKKLLLNLLIVLVVSGILIAVMFTNQSADETVRILKSLDYVYIS
                     IAFACVVINWVFGALIIKAIKKGMIGKQRDPGGNFTAFMASEFAAAVTPLASGSQPAL
                     VYTLSKKGLDTGVATSIAVIRSFIYLVTELALAIIGFLFMRKYLVQHIPHFYAIFIPT
                     IVICLLIVILFLAFLEKGKLANKIVGLFLLLVKRFFPKRYDKIEKAAAESLISFKEGV
                     SVFKTKPWFWFFAFLFQTTEFLFLYLVPVFMLRAIEGTFDNALPLMVCTCICMIVMAY
                     VPTPGTIGGAEAFARLLITPFFVSSPALPVIFIWRVLTYYAKIPFGGICFMVALRGKG
                     AKGKKIKARRTV"
     gene            8656..9219
                     /locus_tag="PLF14_09060"
     CDS             8656..9219
                     /locus_tag="PLF14_09060"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOT71471.1"
                     /translation="MTQLILASTSSRRQEILKKANIPFAILPPDMDEEAFDKSDPKSM
                     VCELAKSKALSVLNKVGNDDIVLGVDTIVYIDNVVLGKPADRADARNMMKMLSGRKHS
                     VISGVCLVGKNGFMKNFHVETEVVFNKLDENEIEEYINTDEPYDKAGGYAIQGLASKF
                     ISGINGCYYNVMGLPLSEIYSVIKNII"
     gene            9231..9905
                     /gene="radC"
                     /locus_tag="PLF14_09065"
     CDS             9231..9905
                     /gene="radC"
                     /locus_tag="PLF14_09065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOT71472.1"
                     /translation="MKELPYDERPYEKLMSKGAQALSDTELLAIIIRTGFNGKNAVDL
                     ARNLLKDKDLGAGLKGLSTAGIQQIMKHKGIGRVKAIQIKASLELANRLNSIKESKIK
                     LTNPDEIARFMLPKCKGLNKEICWILCFDVRMNLISQSVVSVGTLDSSIVHPRDIFTE
                     VIKACAHSFVVVHNHPSGDVSPSENDILLTKRLKKGGALLGMELRDHIIISELNYFSF
                     LSKSML"
     gene            9933..10943
                     /locus_tag="PLF14_09070"
     CDS             9933..10943
                     /locus_tag="PLF14_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015084796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOT71473.1"
                     /translation="MSLFGRDIGIDLGTANTLIYERNKGIILREPSIVAMESKTRKVV
                     AVGNDARAMLGRTPQNIITVKPLKDGVIADYQGTLGMLREFIRKAIGRSLLSKPSMII
                     CVPAEVTKVERKAVIEAGLEAGAKEIFDPEESLMAAVGAGLPVFEPTGNMIIDIGGGT
                     TEVAVISMGNIVNSRTLRSAGDTFDELITQYIKRKYRVIIGARTAEDLKVGIGNATPF
                     DEPKKMVIRGRNADQGLPVNLEVSSEEVAEAMHPVILEILEAIRATLELTPPELAADV
                     MASGIVLSGGGALISGLDKYLSEKTGHIPVKVADKPLECVALGTGKMLDKLDYFKGIT
                     KV"
     gene            10953..>11226
                     /locus_tag="PLF14_09075"
     CDS             10953..>11226
                     /locus_tag="PLF14_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71474.1"
                     /translation="MKLLKNKKFRIILIVAVFVIVAILSINRFSGVNIIRNIVTIPVS
                     WIQKGIYNIDRYFTEKRFTRKQYQELLEENKNLRAENEQLREDNAAM"
BASE COUNT         3405 a         2066 c         2335 g         3420 t
ORIGIN      
        1 aatcctgata ataatttttt tatcctctga ataacccgat ttttcatcgt tcatttcaag
       61 tacatgaaac ttacccagaa cagtggctac aattattttc caggcttcat ccttcgacct
      121 gtcctgtttt acattcaaca caagttcttt atttaggtgt tcttctatgg tctttcgaat
      181 ctgttgttct tttactacca aatcatccac atcaccaata ggttttccta taaacaaaat
      241 atccggttta cttgtgaagt cgacatattc atttcctttt tcacaataaa tatcgtttac
      301 gatatatccg tcatatacat cacgcccgtt ggatttctca tagaggtcgt cgccctgttt
      361 aactgttacc gtatcacgct tgacttcacc tcttctgttt tttatatcca gttccatttt
      421 tgctgtaata ggtgattttt tattatttac cgatataagc ttaatatatg ctttgttatg
      481 ataatctttg gtttcaaagc ccgcaacttc aatctgcttt acaagttcca aattatagct
      541 atccactgca tccagtttat ataccaagtt atgcttttct acatgggtag cagagtaacg
      601 cagagtacat aaaggattta gagacctaat agcttcctta gccttagggg tagtatctac
      661 ggattgcggc tcatctataa tgacaaaagg tcttgtttcc tgtatcagct caataggtct
      721 catcccgctt aacctatcat gggctctatg gatgatatta gctttgtttt ccttagtcgg
      781 gtctttgaaa ctttttctga atgcatcaat gttaataacc atgatttcta tatttgagct
      841 tacggcaaaa cttcttactt gttcaagatt actactgtca taaacaaaga agttgtagat
      901 ggtgttgtca tataactcct gaaaatgttc tttagtaacc tgcaaagact tatatacacc
      961 ttcttttata gcaacacttg ggacaacaat aataaacttg gaaaaaccgt aatttttatg
     1021 caattccaat attgttctta tataaacata tgtcttacct gtgcctgttt ccatctctat
     1081 atcaaaatca tacattccag cttttaaagt cgcagtctgt ggtaatctgt ttctaagttg
     1141 aatattctgt agatttgaca aaatctcttc tttatcaagc tcaagcctgt tacctacacc
     1201 atttgcagta tcaaataaac ctatctgtcc actgtatgct gacacagtaa aattagtatg
     1261 cattggtgtt tgaccactga ataaatcaac aacggaagct atggcttgta gttgatactc
     1321 taaattatca tcaaacttca gtttcattta accatacctc cctaaatagt aatgaattct
     1381 ttgattccac caacttttaa tatttctttg atattggttt tatcgctatc cttttcaaaa
     1441 ccattgtctt tgaatactac tctactgctc tcaggagaca actcttttac tcttttcaaa
     1501 attccttctg caacatcagg cttaattcca ttactaagac aaatcataag cattccataa
     1561 ccgacagaat ataccttttt ctcggaaaca ataaactcat caacagaata agtgaggtca
     1621 aaaccgtatt taagcattat ctcatataca acatcaagct ctgttctgcc ttcaacaaaa
     1681 ttgtccacat agtccaataa ggatacttcc aaattatcat aatcgggttg ccatttgcga
     1741 atgttagacg tatcaagctt gaacacttta aagccaacat caaggttttg tgcaaataaa
     1801 cctgcttcag actttatctt ttcacctgca cgacgaatac gttctttacc tatttcacta
     1861 acatttacat agccagcttt ataagtggta ctttttttgt cggttagttc aggaagttgc
     1921 accataataa atttacgctt tccactatca ccatcatctg cattaagttg cataacagcg
     1981 tgtgcagtag tagctgagcc agagaaaaaa tcgaggataa tgtcattttt ttctgttgca
     2041 tgaaaaatca atctctttat tacatcaata ggctttactg tttcaaatcc atgttcccca
     2101 ataagttcag taaggtcact ctttgcattt tctgccgttc ccaattcttt agagaaaaag
     2161 ccccaaaacg gttctgttgt ctcatttctt tcaaactcag ggcgcatctt aataataact
     2221 ttaccttccc taataaacag attaccacta tctttatatg cggcaacttg gtcttgacca
     2281 agttgccact gtttccctgg cggaggagaa acagtggaaa tgccatcact tacatcataa
     2341 atcattgttt ctctcccgcg aatacctgtt gcattcatca cttcaagtgg atattcgcga
     2401 tagtttcctt gctcatctga caaaggataa tcctttaatt cttttactat aaggttaaat
     2461 cgtcttttta gttgttttgt ataaactaaa atcatttctg cttttggttg aagcttatta
     2521 tatgctgttt cggtattctg tgattttgtt tttgattccc aataaatttc ccctatgaaa
     2581 tttgataaac caaaaatttc atttaatatt gttctgcagt taaaattttc attataatca
     2641 acgctaacaa aaataacgcc atcatcagca agcaggtttc ttgcaagtct aagtctcgga
     2701 tacatcatat ttaaccagtt tgtatgatag cgtccactgg tttccgtgtt tgtgcctatt
     2761 ttttttcctt catcatctac ttgtccggtt attttcttgt agttctcaat gctatcgtgg
     2821 aagttatctg gatacacaaa gtcttcccct gtgttataag gtggatctat atatatcatt
     2881 ttaatcttac catgatagct tttttgtaaa agttttaata cttcaagatt gtcgccttca
     2941 atgtaaagat tttgggttgt atcccagtct ttgctttctt ctttgcaggg tcttaatgtg
     3001 ccaagtgaag gggattgaga taaacgcaaa gcttgtcctt ttccgttcca agtaaaatta
     3061 taccgttcag tactatcttc tacataatcg cctaatactt gtttaagttt atcgaaatct
     3121 actttgcctt cagaaaatac ttccgggaag atttgtttta gcatttgaat atttttttca
     3181 gctatgtcag gcgtctttcc gtccaaggtt gatattttca tggtttgttt tcctccaagt
     3241 agttactttt attatactct aaattcttta tatttttatc caaattctgt tttatgatta
     3301 gatatgtcct ctttattata catattagaa aggcctataa tatacattta atgcttacaa
     3361 caagtaaata tctgccatat accttgtctt ttggtcaaat atggcactga caacatagca
     3421 ttagtgtcaa tttggcacta atgcttgata ttatggcact gttggtttat tatctatacg
     3481 aaaggcggtg ataaaaaatg aacaggtatt atgaatcgga atcagtggaa ttgaaagccg
     3541 gatatacgga taccgttgtg cgggaaatag tttcttttct taacaccgaa ggcggagtga
     3601 tatacattgg tatcgaagat gacggaaccg taataggaat agagaatgta gaaggtgtat
     3661 taagacaggt ttccgactgc ataaccgatc agattgaacc taagccgata ggagaggtta
     3721 taagtgcagt tattcttgaa gataatctgc cgatagttgt tgtcaatgta aaaaaaggaa
     3781 taaaacctat ttactgtatt aaaaagtacg gcttctcctc caaaggctgt ccggttcgta
     3841 taggaaacac ctgcaaagaa atgtctcctg aagaaattca gtaccgctat aagtcgctgt
     3901 ttgtcgatca agactatatg ctgttggcat cggcaaaata tgctcctttg tcttttgaaa
     3961 tgatgaaaat attactcaca ggcagaggtt atcatatcaa cgagagttcc tttgaatctt
     4021 cctttaagct cagaagaaat gacggaacat ataatttgct tgcggaagtt ttggcggata
     4081 agaatatggt acctttaatt tttgtaaagt ttagcggacc ggataagacc tctatttctc
     4141 acagaagcga ttacggcgga cagagtatac ttctcggata tcagaaaatg aaagacaggc
     4201 ttattgcgga gaacatatgt aaaaccgata cttccgttcg accgagaata gatgaatatc
     4261 tttatgatat tgattgtgtc aatgaagcac ttgtcaatat gttggtgcat aacgactgga
     4321 ctgttaccga accgttggtg tccttttatt ccgaccgcgt agtttttaca tcacacggcg
     4381 gtattccgca ggggattacc gaagaagaat tttataacgg agtaagtcaa ccgagaaatg
     4441 cattgttaat gagaatattt cttaatatgg gcatagtgga gcacaccggt cacggagtac
     4501 caatgattgt taaaagatac ggaagagaag tctttgatat tcataaaggt tacatcgatg
     4561 ttacaatacc gtttaacaaa aatgttttgg catcggtgcc aaacagtgtc atcaatgaaa
     4621 gcgctgtcgg ttacggtgta aaagtgtata aggatttgac cgacaccgag aaagaagcaa
     4681 ttttggtatt agtagaaaaa cccgacatta catacgataa gcttgctttg gagattaatg
     4741 tttccagaag aaccgttgca cgcacgatcg cttcattgac agagaaaaaa tatattgaga
     4801 gagtggggaa taataaggtt ggattctgga aagttatacg gtaatcggta ccgtctatct
     4861 ttccagcacc atttccagct ctaccaatgt gccgcctttg acattctgtt ccactgtttt
     4921 tccggtgaat ttaaagccct gttttttata aaagttaata gccttttcat tgcctttaag
     4981 gacgaatata attatgggat ttcggggtaa aacttttata cattgcttca gcagtgcttc
     5041 tcctatgccg tttctctgat atttctgcag aacatatata gccacaactt ccgaagaagg
     5101 tttaatactg acaagctctc tggattcggg tatatacaat gcgaacccga caactttttc
     5161 cttcgcaacg gcaacaaaag tgtttttcgg atattcatat gcatttttta tgcacttctc
     5221 aagacttctg ccgtctaaaa attcctgcgg catcaatccg gtatatgttt cattccaagc
     5281 agtgtaatga acatatgctt ttccgtgttt atcctgatcg gttaccgatt ctcttattat
     5341 aaattccatc acaacaccca taaagcctta atccgtctta ttaccgcaca aatttgtttt
     5401 gtgaaaccta ttatacattg tttttggcgg attataacat tttattgata acatgccgct
     5461 atatatgtat aataaatata aataatatat ataaggagaa taacatggcc gaaatcagtt
     5521 ataaaataga aaaagagata ggtgttcttt ccgaagctcc ttcaggatgg acaagacaga
     5581 taaacctggt aagctggaac ggcagagaag ccaaactgga gattcgtgac tgggctccca
     5641 acagagaaag agccggaaga ggcatgactt ttaccaagga tgaggtcaga aaactggtta
     5701 agtttcttat aaaagcggaa ttggataaat aacatatatt tgttaaagta ttgttccggg
     5761 tctgctgtaa cagttagtta agtcagttat aatatattta tgtttccaac gcattatgct
     5821 tttggtataa tgttttttga ataatttacg ggaaggactt ataaaatgaa gataagtgta
     5881 caaagattat atgacagtcc gaatgactac aaagatatga ttgtgactgt cgaaggatgg
     5941 gtaagaacaa taaggagttc caaagacttc ggtttcatag agttgaatga cggaacctgc
     6001 tttaaaaata tacagatagt gtttgaaaat aatgttgtca ataattatgc cgaggtggag
     6061 aaactgccgg tagcctccag tatttccgtg accggaaaga ttatactgac tcccgatgcc
     6121 aagcagccgt ttgaaataaa ggcggaagat attgagatat gcggtgtcag tgcatcggat
     6181 tatcctttgc agaaaaagag acattcgttt gagtatctgc gtaccgttgc tcacttacgc
     6241 cccagaacca ataccttctt tgcggtattc aaagtgcgtt cggtacttgc tcaggcaata
     6301 cataccttct ttcaggacag aggattcgta tatgtgcaca ccccgataat aaccgcatcc
     6361 gactgtgaag gtgccggaga aatgtttcag gtaaccactc tggatttgga ccgcattgcc
     6421 gggagtaagg aaaaagtgga ttattccaag gactttttcg gaaaacagac caatcttacg
     6481 gtaagcggac agctttcggt ggaaacttac tgtatggcat ttaggaatgt atatacattc
     6541 ggtcccacat tccgtgcgga gaattccaat actcccagac atgcatcgga attctggatg
     6601 attgagccgg agatggcttt tgccgacttg gatgatgata tgagcatggc ggagcagatg
     6661 ataaaataca ttatcaatta tgtaatggaa agatgtccgg atgaaatgga gtttttcaac
     6721 aagttcatag aaccgggact ttttgacaag ctgaaggtag tgaccgattc caaattcgat
     6781 catgtcacat ataccgacgc tataaagatt ttgaaggaaa gtaaagtttt gtttgaaaat
     6841 cccgtggaat ggggatgcga ccttcagacc gagcatgaga gatacctgac agaaaagcat
     6901 tttaaaaagc cggttttcgt taccgactat cccaaagcca taaaggcttt ctacatgaaa
     6961 gtaaatgacg ataacaaaac cgtaagggca atggatcttc tggttccgca agtgggagag
     7021 attatcggag gaagccagag agaagagcgg cacgatgttc ttgtcgccag aatgatagaa
     7081 atgggtttgg ctccggaaga ctactggtgg tatctggata ccagaaaata cggaacggta
     7141 aaacatgcgg gcttcggcct ggggtttgag cggatgatta tgtacatgac cggtatgagc
     7201 aatatccgtg atgtcattcc tttcccgaga gttccgaaac atgcggagtt ttaacaatga
     7261 taaataattc cggtaccgat atagcggaaa gtgtaataat agtaaaaata gcgcagacag
     7321 ttgctctgtt cgataccgta agacttacaa agaagaaaaa agccgtaaaa ctgtccgaca
     7381 gcggaaagct taaaacagtg gaaatttttc tggatattaa ctacaacatt gtaatccccg
     7441 aggttgcatc taaaatacag aaagccgttg caaaagtact ggaaaaggaa ttggatatcg
     7501 tgatggattc cattgatgtg acggttgtgg gtgtcaatat agaaaagttg gggaccgaat
     7561 gaagaaactt ttgctcaatc tgttaatagt attggttgta agcggtatcc tgattgccgt
     7621 aatgtttaca aaccaatcgg cggatgaaac tgtaaggata ttaaagtctt tagattatgt
     7681 atatatatca atagcgtttg cctgtgtggt tattaattgg gttttcggtg cccttataat
     7741 aaaagccatt aaaaaaggta tgataggtaa acaaagagat ccgggcggga attttacggc
     7801 tttcatggca agtgagtttg ccgctgcggt aactccgctt gcgtcgggca gtcagcctgc
     7861 actggtttat acactttcaa aaaagggact ggatacgggt gttgccacat cgatagcggt
     7921 tatacgttcc tttatatatc tggttaccga actggctctt gcaattatcg ggtttctgtt
     7981 tatgcgaaag tatctggtac agcatatccc tcatttctat gcaatattca tacccaccat
     8041 tgttatatgt ctgcttattg tcatactgtt tctggcattt ctggaaaaag gtaaattggc
     8101 aaacaaaata gtgggattat ttttattatt ggtaaagagg ttttttccca aaaggtatga
     8161 caagattgaa aaggcggcag ccgaatcgct aataagtttt aaagagggtg tgagtgtttt
     8221 taagaccaag ccttggtttt ggtttttcgc tttcctgttt cagacaacgg aatttctgtt
     8281 tttgtatctg gttcccgttt ttatgttgcg ggccatagaa ggtactttcg ataatgcact
     8341 gcccttgatg gtgtgcacat gcatctgcat gattgtaatg gcctatgtgc cgacacccgg
     8401 tacgatagga ggagcggagg cttttgcaag gctgcttatc acaccgtttt ttgtttcttc
     8461 tccggcattg ccggtcattt tcatatggag agtgctgact tactatgcca aaataccctt
     8521 cggaggcata tgtttcatgg ttgccttaag aggaaaaggt gcaaaaggga agaaaataaa
     8581 ggcaaggcgg acagtgtgat atagtgtgtg gcactatact gaaatcggca tataatgtca
     8641 attaggaggt tatatatgac gcaacttata ctggcttcta catcatccag aagacaggag
     8701 atattgaaaa aggcaaacat tccgtttgca atactgccgc ccgatatgga cgaagaggct
     8761 tttgataaaa gtgatccgaa gtcgatggtt tgcgaactgg caaagagtaa agctttaagt
     8821 gtattgaaca aagtcggtaa tgacgacata gtcttaggtg tggatacaat agtttacatc
     8881 gataatgttg ttttgggaaa acctgcggac agagccgatg cgaggaatat gatgaaaatg
     8941 ctgagcggca ggaagcatag tgtaatatcg ggagtatgtc ttgtcggcaa aaacggtttt
     9001 atgaagaact ttcatgtgga aaccgaagtg gtgtttaata aactggatga aaacgagatt
     9061 gaagaataca ttaacaccga tgaaccgtat gacaaggcgg gaggttatgc cattcaggga
     9121 cttgcttcca agtttatatc cggtataaac ggatgttact ataacgtaat gggtttaccg
     9181 ttatcggaaa tttacagcgt cataaaaaat attatatgag caattttata atgaaagagc
     9241 tgccttatga tgagcggcct tacgagaagc ttatgtccaa aggagcacag gctctttccg
     9301 acacggaact tttagccatt attataagga cgggattcaa cggtaaaaat gcggtggatc
     9361 tggcaagaaa tctgcttaaa gacaaggatt tgggagcggg acttaaaggt ctttccacgg
     9421 cagggattca gcagataatg aaacataaag gtataggcag ggttaaagcc atacagatta
     9481 aagccagtct ggagctggcc aacaggttaa actcaataaa agagagtaaa ataaagttaa
     9541 ccaaccccga tgaaattgca aggtttatgt tgcccaaatg taaaggactc aataaggaaa
     9601 tctgctggat tttgtgcttt gatgttcgca tgaaccttat cagtcaatcg gtggtatcgg
     9661 tggggacact ggacagttcc attgtacatc cgagagatat tttcaccgaa gtaatcaagg
     9721 cctgtgcaca cagttttgtt gttgtgcaca atcatccttc cggcgatgtc agtcccagtg
     9781 aaaacgatat tttgctgaca aaaagactga aaaaaggcgg agcgctgctg gggatggagc
     9841 ttagggatca tattatcata tccgaactaa attattttag ttttctctct aaaagcatgc
     9901 tataatgtac taaacggaaa ggaagagata atatgtcatt attcggcaga gatatcggta
     9961 ttgacttagg taccgcaaat accttgatat atgagcgaaa caagggaatt atattaagag
    10021 aaccttccat agtcgctatg gaatccaaga ccagaaaggt tgtagcggta ggaaatgatg
    10081 cgagggccat gctgggcagg actcctcaga atatcatcac cgtaaaaccg ttgaaagacg
    10141 gagttatagc ggactatcag ggcactttag ggatgctcag ggagtttatc cgcaaagcca
    10201 taggcaggtc tcttctttca aaaccttcca tgattatatg cgttcccgcc gaagttacca
    10261 aggtggagag aaaagccgta atagaagccg gcctggaagc gggagccaaa gagatattcg
    10321 atcccgaaga aagccttatg gccgcagtag gtgccggatt accggtattt gaacctacgg
    10381 gcaatatgat aatagatatc ggcggcggta ccaccgaagt ggcggttatt tccatgggaa
    10441 atatagttaa cagcaggacc ttgcgttctg ccggtgatac ttttgatgaa cttataaccc
    10501 aatacataaa gagaaagtac agagtaataa tcggagccag aacagccgaa gatttgaagg
    10561 tgggcatagg taatgcaaca ccctttgatg agcccaaaaa aatggtcatc agaggcagaa
    10621 atgccgacca gggactgccg gtaaatctgg aggtaagctc ggaggaagtg gccgaagcca
    10681 tgcatccggt tattctggaa atattggaag cgataagggc tacactggaa cttacacctc
    10741 ccgaactggc tgccgacgtt atggcaagcg gtatagtact gagcggaggc ggtgctctga
    10801 tatcgggact ggacaaatat ttaagcgaga agacaggaca tatccccgta aaagtagcag
    10861 acaagccgct ggagtgtgtg gctctgggaa caggcaagat gctggataaa ctggactatt
    10921 ttaaaggtat tacaaaagta taaggagcag gcttgaaact tctgaaaaat aaaaaattcc
    10981 ggataatact gatagtcgcg gtatttgtta ttgtggcgat attgtcaata aacagattca
    11041 gcggtgtcaa tattataagg aatattgtaa ccatacccgt atcatggatt caaaaaggta
    11101 tctacaatat agaccggtat tttaccgaga agagatttac aaggaagcaa tatcaggaac
    11161 tgctggaaga gaacaaaaat ttgcgggcgg aaaacgaaca gctgcgcgaa gacaatgccg
    11221 ccatgt
//
LOCUS       DAOSOM010000054         9891 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_6824_length_9891_cov_4.117121, whole genome shotgun sequence.
ACCESSION   DAOSOM010000054 DAOSOM010000000
VERSION     DAOSOM010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9891
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6824_length_9891_cov_4.117121"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..1394)
                     /locus_tag="PLF14_09080"
     CDS             complement(141..1394)
                     /locus_tag="PLF14_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT71475.1"
                     /translation="MAFIAENHDFKPFEKKVWLSSPTMHGPELTYMKEAFDTNWMSTV
                     GENINECEKLVAQKVGCTHAVALSSGTAALHLAAKLAGVKPGDVVICSDMTFCATLSC
                     VKYEGATPVFVDTEYDTWNMDPVALEKAFEMYPQAKLVIVANLYGTPGKMDELRAVAD
                     KYGAKIIEDAAESFGAEYKGKQTGTFGDIGVISGNGNKIITGTSGGFLLTNDYAAAAK
                     VRKWSTQSRDNAPWYQHEELGYNYRMSNVIAGVFRGQLPYLEEHIAQKKAIYERYKEG
                     FKGFPIQMNPYDAATSEPNYWLSCMIIDKDAMCPQARDDHKAVYRSERGKTCPTEILE
                     TLEMHNAEGRPIWKPMHMQPIYRTNPFVTVEGNGRGRTNAYIKGSGIDVGADIFRRGL
                     CLPSDNKMTEEQQDRIIEIIHRCFL"
     gene            complement(1545..1693)
                     /locus_tag="PLF14_09085"
                     /pseudo
     CDS             complement(1545..1693)
                     /locus_tag="PLF14_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606175.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
     gene            complement(2014..2595)
                     /locus_tag="PLF14_09090"
                     /pseudo
     CDS             complement(2014..2595)
                     /locus_tag="PLF14_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049093.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            3119..5149
                     /locus_tag="PLF14_09095"
     CDS             3119..5149
                     /locus_tag="PLF14_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71476.1"
                     /translation="MFVRKRPWSSRYGWIWYNSKEILRDTQEDIDALVKDFADKGINI
                     LIGFSCTHFRWNFYRHWNKINECIGKLVKACHKYGIIYVEHHSCHLTFNPLNEDDLVY
                     MKSVLGKRDSYISDWPGLLEDATGDFSVDGKMISSFRQISGKTGKWGISTYHGYGMCF
                     NNPDYVAAYLKYLESLYAVGIDGIMTDDVQYFGNDGMGWREFNACTCAHCREKFKKQY
                     GYDLPSPSEWKDFYWNFNDPVFIAWKRFKDESTLEFVYKVKKHYEDLGLNQFRPNYIS
                     SILTTNKTAYPFEKCADVWDCVFQENCASSVIKESYLSFALESLHRFNLGRLNKIPSM
                     SMFYPVTKDALYFSWALAKSWGQLYTGCGGEGEGGTLDEKWLRDFEIKNMFCYTNPAK
                     ISDLAFMLSFDTRDYSNGCETHQKNFVRWLQASYLSGIATDMIFENAKYKEFAGYKRI
                     VCAHTVMLCDSVLKKLRRYVREGGHLITIGEFGKYRPDGSLRNRIGFGRKSCILGKGK
                     VTVLPDDACCQEYHWVFNIYRMETDHKVATAEAPPYAVDALRATGGKALLDAISYDTV
                     MKVTSKQDVYASLFKVRRGYALNIVNVQDTVTKSGPVSHVAPIPAFVKGAKPIKHDIF
                     VKINMDIPVKKITLHSPERSRCVNIPFVNENGVLTFTIPKNKFAGYALVKIK"
     gene            complement(5174..7072)
                     /locus_tag="PLF14_09100"
     CDS             complement(5174..7072)
                     /locus_tag="PLF14_09100"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HOT71477.1"
                     /translation="MKRLTVILIIILVIGLTACNNEQKPSSEGYENNLLMVYFCGSDL
                     ESKNGRASANIEELLNAYIPDNTKIIIQTGGARKWKNDDINGNFIERFEIADNGLCNV
                     YHQPLNNMGEEETLTEFLNYCAKNYPDTNKSLILWNHGGGALKGCCFDENYNFDSLLI
                     TEIAKALKDADIPKLDFIGFDACLMGTVEVAYILKDCAKYMVASEELEPGCGWDYKAV
                     AESYGKNTLKETLIGIVDSYYEKCEKLGKALNATLSVIDLSEIENLANTFSKLFNTIQ
                     DNIDSVGLYSVTRPVSETSSFGGRTAYESNSNLIDLYGFADNLKLFPDKTDLLKTAIN
                     DCVIYKKNGALKQTCGGLSFYYPYNLNSSELKEMAMLVVSEEYKTFLKDNYADVGKEF
                     IKFEDYGSINESGAFEITLSKQSLKFVREVKYSLLEFAGTEFEPIIASLGTDVDIYNE
                     TPKVYTSNFKGRWVTLNGETLQCSYLGEENGYDIYSSPVKVNGEPKYLRFTFSYATRN
                     FKLLGVWAGISDSGMADKSFDDLKKGDIVTPVYYTFDANYERSTIDGNNIIISENIDF
                     QTSPLKEKYYQYVFVVEDIFGHLYYSRSAVLEMKYTYDELLANPLTGKYDFAADIIAV
                     SDNVALVP"
     gene            complement(7072..9759)
                     /locus_tag="PLF14_09105"
     CDS             complement(7072..9759)
                     /locus_tag="PLF14_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71478.1"
                     /translation="MRIFKKPFLRTLCVFIVLCLAVSLVSCKEKDVKNEYATHLMTGA
                     TAMNDKTYSVTVVTEEESLTFTDVDNANYGMEVVDPYSASKVRDIAQFSIAKNSDTEL
                     NITFTDTDYLAEGQSFRFYSTSPVLSNKSKLDCLIYLQSVSYGLSSDISEFNRYDSNI
                     VLTISLTDGALTDTLSKENINVSGALQDKEYTLKRVDDTTFSLTYVDAFKDDVSDIST
                     ATVTLLAKAIKGNTPFDVSIDFSVITPYAYIDNSAMQFISVEDGTKIIVPVIFDNNFV
                     ITTNPSDMSVTGVSGFTVEKIDLAGDNFMLVTLKTALNREESIIRLDVASLLIKERAV
                     NLGGQKGSIPFGNFNSALSVTALCDYTEQGFNGYVLDIDVINGTFKNYNTITASDIKI
                     KGADGKSYSFTLTSKSEKTLVATVKTTDTTFTGTVSMDSSRINSTFGLMDTEVKGEFI
                     SAYVQDDKGFVSLVLPYLTKAATAAASTLGSTVGSKVATAITPHILNFLGLQQEVTLA
                     AINENITQLGKQLSDLSNQLSSSTATLLNEMSNNAFDDSLTNYNGEYNKLILFALWAY
                     GDGGGLGKLLKMEKDVAGSDGKISDEELTKLLEDSEYIAAQEAFIASFEKNYNIPFLS
                     DIKTFGDNLTSKAVGVGTGYCKAFWGLVQGSYLWDVQTIELKEQFLNTSATAYCLAMS
                     AAIRYLNLKNDTSMLSTLVGSFDFNSKIIENYMKNLDDSMSRKRNNKTLEYSTGKTVS
                     LTLNTYKADKDNNKTTDLSGECNIKFPYENYLAKADFDKIIDAARTKKIDFLRSLRDA
                     GFYGAPKSTEKEVRFGMEYSQSYQEFSTPISARGFSRPAQFYSGSTSSYHISYITCTG
                     NSVSDTNTTTYAKLSRYRERNIKSLIYYDTDNTYYYFMQ"
BASE COUNT         2902 a         2013 c         1833 g         3143 t
ORIGIN      
        1 gcctgcagct cctgaccgtg tccctgtggc tacccaaagc ataaggagca gattcagatt
       61 tacccctgtg gccacctgat gcatatgctg ccgatacagg cactactcga tttgaacccc
      121 tgattcacat tcagccgatg ttacagaaaa cacctgtgga tgatctcaat aatccggtct
      181 tgctgctctt ccgtcatctt gttatcgctc ggtaaacaga gtccacgtct gaaaatatca
      241 gctcctacgt cgattccaga gccttttata taagcgtttg tgcgtcctct gccgtttccc
      301 tcaaccgtga cgaaggggtt ggtgcggtaa atcggctgca tatgcatggg cttccagatt
      361 ggcctacctt cggcattatg catttccagt gtttccagta tttcggttgg acaggtcttg
      421 cctcgttccg atcgataaac agctttatgg tcatctctag cctgcggaca catggcatcc
      481 ttgtcgatga tcatgcagga aagccagtag ttcggctcac ttgttgctgc atcgtaggga
      541 ttcatctgta tggggaaccc tttgaatcct tctttgtatc gctcataaat cgccttcttc
      601 tgtgcgatat gctcctcaag gtaggggagc tgcccacgaa acacacctgc gataacatta
      661 ctcatgcggt agttgtagcc aagttcctca tgctgatacc acggggcgtt gtcgcggctc
      721 tgagtactcc acttgcggac tttagcggca gctgcgtaat cattagttag caggaaaccg
      781 ccggaagtgc cggtaatgat cttattgcca tttccgctga taacgcctat atcaccaaaa
      841 gttccggttt gctttccttt atattctgcg ccaaagctct cagcagcatc ttctattatc
      901 ttggctccat acttatccgc tacggcccgt aactcatcca ttttgcctgg ggtgccgtag
      961 agattggcaa cgatgaccag cttcgcttga gggtacattt caaaagcctt ttccagagcc
     1021 accggatcca tgttccacgt atcatactcc gtgtccacaa aaaccggagt agctccctca
     1081 tactttacac atgagagggt tgcacaaaaa gtcatatctg agcaaatgac cacatcgccc
     1141 ggcttgactc cggcgagctt tgcagcaaga tgcagcgccg ccgttccact ggaaagggca
     1201 acagcgtgag tgcagccgac tttctgggct acaagttttt cgcactcgtt gatattttca
     1261 ccaacagtag acatccagtt ggtatcaaag gcctctttca tatatgtaag ctctggcccg
     1321 tgcatcgtcg gactgctgag ccatactttt ttctcaaatg gtttgaagtc gtggttttct
     1381 gcaataaaag ccatcgtacg ttatcccttt ctctattctg taccccgaca gtaataaatt
     1441 cgttaagacc cctcgggtga attcctttat gataattgca agtttatgcg taaaacactc
     1501 ggtttagcgc caaatatgcg ccgtaatgcg actatttgcc tagttgccgc agttccacat
     1561 aaaagtgcaa cagcgtattt acacccgaat tttacgccgc aagacgctcg atcccgttaa
     1621 tgtttttccc gaccgttgac ctccagtttg tctgaaaagc cttggtcata tattgaagtt
     1681 cctcatcgtg catcgtcggc gaggacaacc agacttaatg atggaaagaa ttcaaataca
     1741 tataatctcc tcttatacca aaaagcagcg tttaaatcca cttttttaac agcaattact
     1801 tcaacgtttt cataaatctg tctctataaa caccttgatg ttatgaagat aattacatta
     1861 cgttatttaa ttcgtatcag aaacttatct ttgataatat ccgaacggta tgtaatcttg
     1921 atttatgtag caatcgatcc acataatcaa gccgataagc ccttatttca gcccttttta
     1981 agccgttttt gcttcataat gccctgtata aaacttgtcc tcatgtaaac tatatatgcc
     2041 atatttccca atagttccag tatgcttatc tttgtaatat gcacccaaag actcggcagc
     2101 atcttcgatt acaggcacat tgtatttatt acatatatcc attatttcat ccatctttgc
     2161 tgatagacca tataaatgaa caaccaatac agcttttacc ctacctttat atttactcaa
     2221 agctttcttc aataaaaatg gatccatatt ccatgattca taatcactat caataaatac
     2281 aggagttgca ttctggtaga taataggatt tgctgtcgca gaaaaggtta gagaaggaca
     2341 taatacaata tcaccttctc ccacaccaat tgccttcaaa gccatatgaa tagctgcagt
     2401 tccggaaacc aaagctgcag catattttgc tccaacctta ttggctattt cattctcgaa
     2461 ttccgtaaca tttggaccta aaggagctat ccaattagta tcgaatgcct ctttaatata
     2521 gaacttttca tatccctcct cactcatgtg tggagaagaa agccaaatct tttctttact
     2581 actatctttt atcattatct ctttaaccgt caatataact ataacagcat cagtaaaatt
     2641 agattatctc taaatagaac aagaattgat ataaccaatt tatacatact tctgttcaaa
     2701 aaaagaacaa agtacattac taatatatat tttttaattg ttgttaattc tctgcatccc
     2761 actctatcga tgctgtagca gcgataaaca gttgtgcata tatttcaccc atattcaact
     2821 acgtgctccg ggcttcgtta gttgtttaca gggtcacggg caaacaaaat gttcatatgt
     2881 gattatacca ttatattaat atacataaca agtacattat ctgtttaata tattcatatt
     2941 gttatgttta cctttcattc cttgtatttt ctgtcatctt tagtggtcaa tatgctttaa
     3001 taaaacttag tattatttaa tattgtttta atattgtttt agaaaactgc ctcattgtgt
     3061 atgtttttta tgcatgttat acttattctg tcaatatatt ttacgggggg aaaagaatat
     3121 gtttgtaagg aaaagaccat ggagtagcag atacggttgg atatggtata actctaagga
     3181 gatattaaga gatacacagg aagatataga tgctttggta aaagactttg ccgataaagg
     3241 tataaatata ctgatagggt tttcctgtac acattttagg tggaactttt atcgtcattg
     3301 gaacaagata aatgaatgta taggtaagct tgtaaaagca tgccataaat acggaataat
     3361 ttatgtagag catcacagct gtcatctgac atttaatcct cttaatgaag atgatttagt
     3421 gtatatgaag agtgtattgg gaaaaagaga ctcatacata tccgactggc cgggactatt
     3481 ggaagatgca actggagact tttccgtaga cggtaaaatg atatcatcat ttcgccagat
     3541 ttccggtaaa accggaaaat ggggaatatc aacatatcac ggatacggaa tgtgttttaa
     3601 taatcctgat tatgttgcag catatcttaa gtacttagaa tcgttgtacg ctgtaggaat
     3661 agacggcata atgaccgatg atgtgcagta cttcggaaac gacggtatgg gttggagaga
     3721 attcaatgcc tgcacttgtg ctcattgcag agaaaaattt aagaaacaat atggatatga
     3781 tttaccctct ccttccgaat ggaaagactt ttactggaac ttcaatgatc ccgtatttat
     3841 agcatggaag agattcaaag atgaatccac actggagttt gtgtataaag taaagaaaca
     3901 ttatgaagat ttgggtttaa atcaattccg tcccaactat atatcttcca tacttacaac
     3961 aaacaagacg gcatatccgt ttgaaaagtg tgccgatgta tgggattgcg tattccagga
     4021 aaactgtgca agttccgtta ttaaagaatc ctatttaagt tttgcactgg aatccttaca
     4081 cagattcaat ctgggaagac tgaataaaat accttccatg tccatgttct atcccgtaac
     4141 aaaagatgct ttgtatttct cctgggcact ggcaaaatcc tggggacaac tgtataccgg
     4201 ttgcggagga gaaggagaag gcggaacact tgatgagaaa tggcttcgtg attttgaaat
     4261 aaagaatatg ttctgttata ccaatcctgc gaaaatatcc gatctggcat tcatgctatc
     4321 cttcgataca agagattatt caaacggatg cgaaacccat cagaagaatt tcgtgcgatg
     4381 gttgcaagct tcttatcttt cgggtatagc tacagatatg atttttgaga atgccaagta
     4441 taaagaattt gcggggtata aaaggattgt atgtgcacat acagtaatgc tttgtgattc
     4501 ggtattaaag aaactgagaa gatatgtcag agaaggagga cacctcataa ccataggaga
     4561 attcggaaag tacagacctg acggatcttt aagaaacaga atcggtttcg gaagaaagtc
     4621 atgtatttta ggtaaaggta aagtaacggt acttcccgat gacgcatgct gtcaagagta
     4681 tcactgggta ttcaatattt acagaatgga aaccgatcac aaggtagcaa cagccgaagc
     4741 acctccgtat gccgtagatg cattaagagc tacgggcggt aaagcactgc tggatgcaat
     4801 atcttatgat accgtaatga aggtaacaag caaacaggat gtttatgcaa gtctgtttaa
     4861 ggtaagaaga gggtatgctt taaatattgt aaatgtacag gataccgtta caaaatcggg
     4921 acctgtaagc catgtggctc ccatacccgc atttgtaaaa ggtgccaaac ctataaaaca
     4981 cgatatcttt gtaaagatta atatggatat ccccgttaag aagataacgt tacattctcc
     5041 tgagagaagc agatgcgtaa atataccctt tgttaatgag aacggggttc ttacctttac
     5101 aatacccaag aacaagtttg cgggatatgc tcttgttaag ataaagtagc attaagagtt
     5161 tcagatattt ttattaaggc acaagtgcaa cattatctga aactgctatg atatctgctg
     5221 caaaatcata tttacctgtt aacggatttg ctaacagttc atcatatgta tacttcattt
     5281 ccagaaccgc actacgggaa taatagaggt ggccgaatat atcctctact acaaagacat
     5341 attggtaata tttttcttta agcggtgagg tttgaaaatc tatattttcc gaaattataa
     5401 tattattgcc gtctattgtt gaacgttcat agtttgcatc aaaagtgtaa tatacgggag
     5461 ttactatgtc tcccttttta agatcgtcaa aggatttgtc cgccataccc gaatcactta
     5521 tacctgccca tactcctaat aatttaaagt ttcttgttgc gtaggaaaat gtaaaacgaa
     5581 gatatttagg ttcaccgttt actttaacag gcgatgaata aatatcataa ccgttttctt
     5641 cacctaaata ggagcattgc aaagtttcac cgttaagtgt tacccatctt cccttaaagt
     5701 tacttgtata aactttcggt gtttcgttgt atatatccac atcggtgcct aaagatgcaa
     5761 ttatcggttc aaattcggtt ccggcaaatt ccaacagtga atatttgacc tcacgtacaa
     5821 atttcagaga ttgtttgctt agagttattt caaaggcacc tgattcatta attgaaccgt
     5881 aatcttcaaa ttttataaat tccttaccga catccgcata attatctttc aaaaaggttt
     5941 tatactcttc cgacacaaca agcattgcca tctcttttaa ttcactactg ttaaggttgt
     6001 agggataata gaaagaaagc cctccgcaag tttgttttag cgccccgttc tttttataga
     6061 taacacagtc gttaatagcg gttttgagca aatccgtttt gtcgggaaaa agctttagat
     6121 tgtccgcaaa accgtatagg tcgataaggt tactgttgga ttcatatgca gtcctgcccc
     6181 cgaatgaaga agtctcggac acaggacggg taacggaata cagaccgaca ctgtcaatat
     6241 tatcttgtat agtattaaac agtttgctga atgtatttgc cagattttca atttcagata
     6301 aatcaattac cgaaagagta gcattaagcg cttttccgag tttttcacat ttttcataat
     6361 acgagtctac aatgccgatt agagtttctt ttagagtgtt ttttccgtaa ctttccgcaa
     6421 cagctttgta atcccagccg caaccgggct ccaactcttc gcttgctacc atatatttcg
     6481 cgcaatcttt taagatatat gcgacctcaa cggttcccat aaggcaggcg tcaaaaccta
     6541 taaagtccaa cttaggtata tcggcatcct taagtgcttt agcaatttcc gttatcaaaa
     6601 ggctgtcaaa attatagttt tcatcgaaac agcaaccttt cagtgccccg ccgccatggt
     6661 tccagagaat taaggattta ttggtatcag gataattttt tgcacaatag ttaagaaatt
     6721 ccgttaaagt ctcctcttct cccatattgt tcagcggctg atgatataca ttgcataatc
     6781 cgttatccgc tatttcaaaa cgctcaataa agttaccgtt tatgtcgtca tttttccact
     6841 ttctcgctcc ccctgtctga attattatct tagtattatc ggggatatag gcgtttaaaa
     6901 gctcctcaat atttgcactt gctctgccgt ttttgctttc caggtcgctt ccgcaaaaat
     6961 ataccatcag aaggttattc tcataacctt ccgatgacgg tttttgttcg ttattacagg
     7021 ccgtaagacc gataacaaga attatgatta agataacggt taagcgtttc atcactgcat
     7081 aaaatagtaa tatgtgttgt cggtatcgta ataaataagt gatttaatgt ttctctctct
     7141 atatctggac agctttgcat aagtggtcgt attcgtatca cttacactat tacctgtaca
     7201 ggtaatataa ctaatatggt aactgcttgt tgatcccgaa taaaactgtg caggtctgct
     7261 aaatcccctc gcagagatag gagtactgaa ttcctggtac gattgagagt attccatacc
     7321 gaatcttacc tctttttccg tgctcttagg tgccccgtaa aaacctgcat ctcttaaaga
     7381 ccgcaaaaaa tcaatttttt tagttcgagc tgcatctata atcttatcaa aatcagcttt
     7441 ggcaagatag ttctcatagg gaaactttat attacattcg ccgcttaagt cagtagtttt
     7501 attgttgtct ttatccgctt tataggtatt gagagttaaa cttacggttt tgcctgttga
     7561 gtattccagc gtcttgttgt ttctttttct tgacatggaa tcatccaggt ttttcatgta
     7621 gttttctatt atcttggagt taaaatcaaa actacctaca agagtggaaa gcattgaagt
     7681 atcatttttc aaattcagat aacgaatagc ggcagacatc gctaagcaat atgcggtggc
     7741 ggaagtattg agaaactgct ctttaagttc aatagtctga acatcccaga gataagaacc
     7801 ttgtaccaac ccccaaaatg ctttacagta ccctgttccg acaccgacgg cttttgaagt
     7861 aagattatct ccgaatgttt taatatcgga aagaaagggt atattatagt ttttttcaaa
     7921 actggctata aaagcctctt gtgccgctat atactcggaa tcctctaaaa gtttagtaag
     7981 ttcctcatcg gaaatttttc cgtcggaacc ggcaacatcc ttttccattt tcaataactt
     8041 acccagtccc cctccgtcac cgtaagccca taaagcgaag aggataagct tattatactc
     8101 accgttatag tttgtgagtg aatcatcaaa tgcattattg ctcatttcgt tcagtaaagt
     8161 tgcagtggaa gaagaaagct gattacttaa atcgctcagt tgttttccaa gttgtgtgat
     8221 attttcatta atagcagcta atgttacctc ctgctgaagc cctaagaaat tgagaatatg
     8281 cggtgtaatc gcagtggcga ctttgcttcc tacagtgctt cccaaagtac tggcagctgc
     8341 agttgcagcc ttggtcaaat agggtaaaac aagagataca aaacctttat cgtcctggac
     8401 atatgcggaa ataaactccc cttttacttc cgtgtccata agaccgaagg tgctgtttat
     8461 cctggaactg tccattgata ccgtaccggt aaaagtagtg tcggttgttt ttactgttgc
     8521 aacaagtgtt ttttccgatt ttgatgttaa tgtaaagctg taagacttac cgtccgcacc
     8581 ttttattttt atatcgctcg cagttattgt gttatagttt ttgaaagtac cgttaattac
     8641 atctatatca agaacatacc cgttaaatcc ctgctccgta taatcacaaa gagcggtaac
     8701 ggacagagca ctattgaaat tgccaaacgg aatgctgcct ttttgcccgc cgagattgac
     8761 ggctctttct ttaatcaaaa gacttgcgac atccagtctt attatacttt cttccctatt
     8821 aagagcggtt ttcagtgtaa caagcataaa gttatcaccg gcaagatcaa tcttttctac
     8881 tgtaaagccc gatactcccg ttaccgacat atcggaagga ttggttgtta taacaaaatt
     8941 gttgtcaaaa ataacaggca caataatctt ggtgccatcc tctacgctaa taaattgcat
     9001 agcagagttg tcaatatatg cataaggtgt aattaccgaa aaatctatag atacatcaaa
     9061 cggagtattt ccctttatag cttttgcaag taatgtaacc gtagctgttg aaatatccga
     9121 tacatcatct ttgaaagcat caacataagt taaagaaaat gttgtatcgt caactctttt
     9181 aagtgtatat tctttatctt gcaatgctcc gcttacatta atattttctt ttgacagagt
     9241 atcggttaaa gcaccgtcgg taagagatat tgtcagcaca atattgctgt catagcggtt
     9301 aaattcactt atgtcgcttg aaagtccgta ggaaacggat tgtaaatata ttaagcaatc
     9361 caatttgctt ttgttactta atacgggtga agtactgtaa aatctgaagc tttgaccttc
     9421 cgccagatag tcggtgtccg taaaagtaat gttaagttcc gtatcagaat tttttgcaat
     9481 actgaactgt gctatatcac gaactttaga tgcgctataa gggtcgacaa cttccatacc
     9541 gtaatttgca ttatcgacat cggtaaaagt caaactttcc tcttcggtaa ctaccgtaac
     9601 cgaatatgtc ttatcattca ttgcggttgc tcctgtcatc aggtgtgtag catattcatt
     9661 tttaacatcc ttttctttgc atgataccaa agaaacagcc agacaaagca ctataaatac
     9721 acataatgtt cttaaaaaag gtttcttgaa aattctcatt atcatcatcc tccatatttt
     9781 atataagttg tgtgacacat agtttattaa taccgtttta ccgctttacc gtttttgtgc
     9841 tgcggcttca accggatttt attatatatc atcgttactt tcttttaaag a
//
LOCUS       DAOSOM010000055         9857 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_6856_length_9857_cov_5.236176, whole genome shotgun sequence.
ACCESSION   DAOSOM010000055 DAOSOM010000000
VERSION     DAOSOM010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9857
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6856_length_9857_cov_5.236176"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..1921
                     /locus_tag="PLF14_09110"
     CDS             14..1921
                     /locus_tag="PLF14_09110"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOT71479.1"
                     /translation="MKKPELFLLGVDGGMTSYITEQIEKGKLPNLKKAFSEGCVLTDC
                     RPAYPSISATCWATIQTGASPHMHGITDENIVPEGAYPNEFVSAYHGDYLMAERFWEA
                     AARIGKKSLIADFCVSGPARTHLARTIGGIGSVADSRVLATEQTLRTVKEISEQLYVF
                     DADVSSIFPSLPAFAMTASGKWQPQIKLQQSKYDKTDKGYFRLHVEKKNDLENPFNIE
                     EFYWYMDVTKKGINIAVSEKDMQAGKYYEVPMNGWSEVIKRTLKDDNGPGNYRFRIKL
                     IPAGENGGFMVFITSAVNMYNALSPESFAKRADNIEEICSSNSYAHFLTREGNIDTAF
                     ELIEFKNEWWKKIILDAMENDPVDIMAFVMGHTDSVNHMYRSIFEGLQNPTEEQSAVA
                     KYAYKRAYEILDDFIGWLYEHVLGEDTVFMIIGDHGSIGFNRTVLVHDYLEKEGLLNY
                     LPEPDGKRLYWRNRRIDWKNTKAFPVGCVNVYVNLKGRQPEGIVEPEDYDKTVNEIIR
                     ALYKHHWNEKDQCYIAFAVEKQQAGFVGLGTERVGDVLYGLSGNTTGGYIGGVHACQI
                     PSAKTATGDIRCLFALSGKNVKKRQIIDRPANIEDVAPTLCYLLKYPQPAQATGGVLF
                     HLFEDDMYQKS"
     gene            complement(1909..2625)
                     /locus_tag="PLF14_09115"
     CDS             complement(1909..2625)
                     /locus_tag="PLF14_09115"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HOT71480.1"
                     /translation="MLVTVIGRGHSGTRAMSQTLSESGVYMGEPLNTSWDLLPPQDMY
                     EACRVFGKYVRYTGNMKWDFSRVMAMDPDPAFVKLIESYLKSVLTSDRDKKGWKIPET
                     TLVYPWIRKMFPKAYYIHWVRDPRDCILKKHMTDDLNDFGIEYDKTEDVRRNRAISWY
                     YQRELVRSTPVAQREIHIRFEDMIFKQDETLKRLEQFLHIPMAKIPMRTDSVGRYKTD
                     EEVHMFDFFKKDMEECGYDF"
     gene            2954..3532
                     /gene="cysC"
                     /locus_tag="PLF14_09120"
     CDS             2954..3532
                     /gene="cysC"
                     /locus_tag="PLF14_09120"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HOT71481.1"
                     /translation="MMTNVVWHRNLDSKYRQEKTGNKGMVIWFTGLSASGKSTIASLV
                     EKNLIDSGKTAYLLDGDNVRHGLCSDLAFSERDRVENIRRLAEVAALFEDSGVIAIVS
                     AITPMEKMRKLARSKAKHFVLVYVKADLDTCIERDPKGLYEKALKGEIKGYTGIDSPF
                     EQPEQYEILLDTDKYDVLTCANKVTEYIQGVL"
     gene            3532..4413
                     /gene="cysD"
                     /locus_tag="PLF14_09125"
     CDS             3532..4413
                     /gene="cysD"
                     /locus_tag="PLF14_09125"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009336 - sulfate adenylyltransferase
                     complex (ATP) [Evidence IEA];
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA];
                     GO_process: GO:0070814 - hydrogen sulfide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HOT71482.1"
                     /translation="MDHLDRLEAKSIHIMREAYSCFKNLCMLWSIGKDSTVLLCLARK
                     AFYGHVPFPLVHIDTRYKIPSMVEYRDKLALEWNLDMIYGENTLALQEKRTFPDGNCT
                     RLECCKNLKTEALIHTLSGEWTRYRLDHKLKKYVPDPNTEPFTGVIVGVRADEEGSRS
                     KERYFSPRDTHSDWDISDQPPEFWGQFKTDFAPGTHLRIHPLLDWTELDIWEYIKRER
                     VPIVPLYFDQGNNTRYRSLGCYPCTKPVASSAKNIDDIIEELKTGKFANIAERSGRDQ
                     DKEDGGGLEELRKGGYM"
     gene            4414..6204
                     /locus_tag="PLF14_09130"
     CDS             4414..6204
                     /locus_tag="PLF14_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOT71483.1"
                     /translation="MTENNNEHMNIVIVGHVDHGKSTVIGRLLADTGSLPEGKLEQIK
                     ELCRRTSKPFEYAFLLDALKDERSQGITIDTARSFFKTDKRKYIIIDAPGHIEFLKNM
                     ITGASRADAALLVIDAAEGVKENSRRHGYMLSMLGIKQVCVLVNKMDNVSYSEKAYLK
                     IVSEYDAFLKQIGIVAEGFIPVSAMQGDNIAKTSINMPWYKGDSVLTMLDKFRATKPL
                     DYLPFRMPVQDVYKFTADNDDRRIIAGTVESGILKQGDQVVFYPSGKKTHVKRIEPMS
                     EQEEVGKASAGYATGFTMTEQIYVRRGELCVKADELPPLMGKHMKVSLFWLGKNPMVK
                     DKKYYLKTGTMKAECYLEEILSVMDASTLVKTEKQYIDRHDVADCIISTSRLCAFDNV
                     AALASTGRFVIVDDYEISGGGIFTDTVENLHRYERAKVMLREGKWEHGYVTVEERSDK
                     YDQNPALIILTGAKDTNKKLFAKALERTLFDEGRNVYYLGIGSVIYGLSADINPPGEK
                     ESRSGENIRRLAEVANILTDTGLILIVTASDLTKYDIETINLVTKNDNMLVIWLGEEC
                     TTDIECDMVLRADEDALEIIRKRVEQITAR"
     gene            6209..6994
                     /locus_tag="PLF14_09135"
     CDS             6209..6994
                     /locus_tag="PLF14_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOT71484.1"
                     /translation="MGIIENIRQFILQTPSNFWVFILVGFIAQVIDGTLGMAYGVSCN
                     SFLLTFGVSPAIASASVHIAEVFTTSVSGFSHWRLKNVDKSMFLKLLIPGVIGGAVGA
                     YVLSNFEGGTIKPFINAYLIVMGVVIIVKIFKKQKEKHLGGWIYPLGLAGGVLDAIGG
                     GGWGPVVTSTLIATGVEPRYTIGTVNAAEFFVTLAESIMFISFLGLQDSVYAILGLVI
                     GGIIAAPFGAFLCKRVPMKPLMALVGALVIFLNVKSLIEFFIK"
     gene            7002..7676
                     /gene="pnuC"
                     /locus_tag="PLF14_09140"
     CDS             7002..7676
                     /gene="pnuC"
                     /locus_tag="PLF14_09140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HOT71485.1"
                     /translation="MFKDWKIGDFIFYFASIILVVVSGIIFKAYWVSFLVSTVGITAG
                     VIHMKANKYSYILYIIQISLYAYVSWRNRFIGEAVLAAFYLLPLYIYSAIKWIKLGNK
                     EKGDAQIFKITKKQIIILAVACVVVTGGYGYVLSLLKSNLPYANALGTFLSVAAGYLS
                     AKRVKEQWLFWISYSLIFSGIWISTLGSDTSNVTLIVQNIPYLYINISGLIKWNKLYK
                     EAHAEV"
     gene            complement(7703..8470)
                     /locus_tag="PLF14_09145"
     CDS             complement(7703..8470)
                     /locus_tag="PLF14_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HOT71486.1"
                     /translation="MSYDIVHAFNKDNLDTYLKALAKLFRKQNGKRATAEIILVGGAA
                     VLVNYGFRDMTADIDAIIHASASIKDAINQIGDEYNLPNGWLNTDFMHTASYSEKLNE
                     FSVYYKTFYGILQVRSISAEYLIAMKCKSGRKYKNDMSDIIGILAEHKKREEPLTWEK
                     VNQAVINLYQDWNGIAKEIKDFIRNAVMTDNYEEVLKQIKEQEQLTKNILIQFQKEHP
                     HTVTESNVDSILRMLESKKIADNIEGKNRICTEDEML"
     gene            complement(8460..9029)
                     /locus_tag="PLF14_09150"
     CDS             complement(8460..9029)
                     /locus_tag="PLF14_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT71487.1"
                     /translation="MTVRELIENKNITLYRLSKNSGIPYTTVNDIYHGRTGLDKCTAE
                     TVYKLSKELGVSMEELLIPYLSEPTDFELYKSSVCHRLKELGDIDFIAETLQHDNISK
                     LYHRKKYPESLYLLAMLDYVSRVNNIPLCTKYKALRNVKLEETLYPAGILAMAVISGQ
                     SKIKEDALSQSVPEFLRHNIIESEVRNVI"
     gene            9215..9288
                     /locus_tag="PLF14_09155"
     tRNA            9215..9288
                     /locus_tag="PLF14_09155"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9247..9249,aa:Gly,seq:ccc)
     gene            complement(9657..9752)
                     /locus_tag="PLF14_09160"
     CDS             complement(9657..9752)
                     /locus_tag="PLF14_09160"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOT71488.1"
                     /translation="MLDQKLFTLIAVAEEKNFTKAAEKLSLTQPV"
BASE COUNT         2995 a         1805 c         2238 g         2819 t
ORIGIN      
        1 gaggcgggat atcatgaaaa aacctgaatt atttttactg ggagtggacg gaggaatgac
       61 ttcgtatata acggagcaga tagagaaagg taaactgccc aatcttaaaa aagctttttc
      121 cgaaggctgc gttctgaccg actgcagacc ggcgtatcct tcgatatccg ctacctgctg
      181 ggctaccata cagaccggag caagccctca tatgcacggt ataaccgacg aaaatatcgt
      241 accggaaggt gcatatccca atgaatttgt ttccgcttat cacggtgatt accttatggc
      301 agagcgattc tgggaagccg ccgcaagaat aggcaaaaaa tccctgatag cggatttctg
      361 cgtatcgggg ccggcaagaa cccatctggc ccgtactatc gggggtatag gcagcgtagc
      421 cgattccaga gtgcttgcaa cggaacagac cttacgtacg gtaaaggaga tatccgaaca
      481 gttgtatgta ttcgatgccg atgtctcatc aatatttccc tctttgcccg cctttgcaat
      541 gaccgcttcg gggaaatggc aacctcagat aaaacttcaa cagagtaagt atgataaaac
      601 cgataaagga tacttcagac tgcatgtgga aaagaagaat gatttggaga atccgtttaa
      661 tatagaggag ttttactggt acatggatgt aacgaaaaaa ggaatcaata ttgccgtcag
      721 tgaaaaagat atgcaggcag gtaaatatta tgaagtacct atgaacggct ggtcggaagt
      781 tataaaaagg acattgaaag acgataacgg accgggcaat taccgattca ggataaaact
      841 tatcccggca ggggagaacg gcggttttat ggtatttatc acttccgcag tcaatatgta
      901 taatgcatta tctcctgaaa gctttgcaaa aagagcggac aatattgagg agatatgctc
      961 ttccaacagt tatgcccatt ttcttacccg ggaggggaat atagatacgg cgtttgaact
     1021 tatcgagttc aagaacgagt ggtggaagaa aataatattg gatgcaatgg aaaatgatcc
     1081 tgtggatata atggcgtttg taatgggaca taccgacagc gtaaaccata tgtacagaag
     1141 tatctttgaa ggattgcaaa atcccaccga agagcaatcg gctgttgcaa agtatgcata
     1201 caaaagagca tatgaaattt tagacgattt tataggatgg ctgtatgagc atgttttagg
     1261 tgaagatacc gtatttatga taataggcga tcacggctcc ataggattta acagaaccgt
     1321 acttgtacat gactatctgg aaaaagaagg tcttttgaat tatctgcccg aaccggacgg
     1381 aaagcgtctt tactggcgca acaggcgtat agactggaag aatacgaaag cttttcccgt
     1441 gggttgtgtc aatgtgtatg ttaatttaaa aggtcgccag ccggaaggta tagtggagcc
     1501 ggaagattac gataagacgg taaatgagat tataagagcg ctgtataagc accactggaa
     1561 tgaaaaagac caatgctata ttgcttttgc tgtagaaaaa cagcaggcgg gatttgtagg
     1621 cttgggaacc gaaagagtgg gagatgtgtt atacggtctt tcgggaaaca ctaccggcgg
     1681 atacatcgga ggagtgcatg cctgtcagat accctcggca aaaaccgcta cgggagatat
     1741 ccgttgctta ttcgcactat caggaaagaa tgtgaaaaaa aggcagatta tagacagacc
     1801 tgccaatatt gaggacgttg cgcccacttt atgctattta ctgaaatacc ctcagcccgc
     1861 tcaggcaacg ggcggtgtgt tattccacct ttttgaagac gacatgtatc agaagtcgta
     1921 accgcattct tccatgtctt ttttaaaaaa gtcaaacata tgaacttcct catcggtttt
     1981 gtatcgccct acggaatcgg tccgcatggg gatttttgcc atgggaatat gcaaaaactg
     2041 ctccagtctt ttcagagttt catcctgctt aaaaatcata tcttcaaatc ggatatgtat
     2101 ctctctttgt gcaacggggg tgctccttac cagttctctt tgataatacc agcttattgc
     2161 acggtttctt cttacatcct ctgttttgtc atattctatt ccgaaatcgt taaggtcatc
     2221 ggtcatgtgt tttttcaata tgcagtctct gggatcgcgt acccagtgta tgtaataagc
     2281 ttttggaaac atcttccgta tccagggata aaccaatgtt gtttcgggta ttttccagcc
     2341 ttttttatcg cggtccgagg taagaaccga cttaagataa ctttctataa gctttacgaa
     2401 agccgggtcg gggtccattg ccataacgcg tgaaaagtcc catttcatgt tacctgtgta
     2461 tctgacatat ttgccgaaaa ccctgcatgc ttcatacata tcctgcgggg gaagaagatc
     2521 ccatgaagtg tttagcggtt ctcccatata tacaccgctt tcgcttaaag tctgcgacat
     2581 tgctcttgtg cccgaatggc ctcttccgat aactgttact aacataaaaa atacctcgcc
     2641 ttaaagtgta ctttacaaca aagataagtt atatgcaaca tcaagagagc atgtttaagc
     2701 taaattagat tgcataacta agttttgttt tacagggcat gcaaaaatca gttttgcaat
     2761 ttaacagttt cgagtataat cgaataaaca tattgttgca gcaataaatt cgactataat
     2821 cgaatattgc gcaaaatcga ttattacgat tagaaatgtt atagatacta tagatgtgtt
     2881 tttacaggtt tcttttgttt gacaaacata ataggtgcat atacaatcac aattggcaaa
     2941 tgtggaggac gcattgatga ctaatgtcgt atggcatcgc aacttagaca gcaaatacag
     3001 acaggaaaag acaggcaaca aagggatggt tatctggttt accggtcttt cggcaagcgg
     3061 aaaatccaca atcgcttcgt tggtggaaaa gaaccttata gattcgggta aaacggcata
     3121 tttgcttgac ggagataatg tccgtcacgg tttgtgttcc gatttggctt tttccgagcg
     3181 tgacagggtg gaaaatatcc gaagattggc agaggtggct gctttgtttg aagacagcgg
     3241 agttatagcc atagtcagcg ccattactcc catggaaaag atgagaaagc ttgcaagaag
     3301 caaggcaaaa cactttgttt tggtatatgt caaagccgat ctggatacct gtatagaacg
     3361 cgatcccaaa ggactttacg aaaaagcttt gaaaggcgag ataaaaggat ataccggcat
     3421 cgattcaccg tttgagcagc cggagcaata tgagatttta ttggatacag ataaatatga
     3481 tgttttgaca tgtgcaaata aagtaacgga atatatacaa ggagtactgt aatggatcat
     3541 ctggacaggc tggaagcgaa gagtatacat ataatgcggg aggcatacag ttgctttaaa
     3601 aacttatgta tgctgtggtc gataggtaaa gacagcaccg tgcttttatg ccttgcaaga
     3661 aaggcttttt acggccatgt gccctttccg ctggttcata tagatacccg ttataaaata
     3721 ccgtccatgg tggaataccg cgataaattg gcgctggaat ggaacttaga catgatatac
     3781 ggtgagaata cgttagcttt gcaggaaaaa agaacttttc ccgacggaaa ctgcaccaga
     3841 ctggaatgtt gtaaaaactt aaaaaccgaa gcgttgatac atactttatc cggagagtgg
     3901 acacgttaca gattggatca taaactgaaa aaatatgtac ccgaccccaa taccgaaccg
     3961 tttacggggg taatagtggg agtgcgggcg gacgaagagg gcagccgttc caaagaacga
     4021 tatttttctc ccagagatac tcacagtgac tgggatatat ccgatcagcc tccggagttc
     4081 tgggggcagt tcaagacgga tttcgctccc ggtacccatc tgagaataca tcctttgctg
     4141 gactggacgg aactggatat atgggagtat ataaaacggg agagggtgcc tattgtgcct
     4201 ttgtactttg atcagggaaa taataccaga taccgttctt tgggctgtta tccctgtaca
     4261 aaaccggtag catcatctgc taaaaacatc gatgatatca ttgaggagct taaaaccggt
     4321 aagtttgcca atatagccga acgttcggga agagaccagg ataaagaaga cggcggcggt
     4381 ctggaagagt tgagaaaagg cgggtacatg taaatgactg aaaacaacaa tgaacatatg
     4441 aatatagtca ttgtagggca tgtagaccac ggcaaaagca ccgttatagg gcgattattg
     4501 gccgatacag gctctctgcc cgaaggaaaa ctggagcaga ttaaagaact gtgcagaaga
     4561 acaagcaaac cttttgaata tgcattttta ctggacgcat taaaagatga gcggtcacag
     4621 ggcattacca tagataccgc cagaagtttt tttaaaacgg ataagagaaa gtacataatt
     4681 attgatgcac ccggacatat agagtttctt aaaaacatga ttaccggagc ttccagagcc
     4741 gatgctgcac ttttagttat agatgctgcc gagggtgtca aggaaaactc cagaagacac
     4801 ggatatatgc tttccatgtt gggtataaag caggtgtgcg tactggttaa taagatggat
     4861 aatgtgtcat acagcgagaa agcatacttg aagattgttt cggaatatga tgcttttctt
     4921 aaacagatag gcattgtagc ggaaggattt ataccggtaa gcgcaatgca gggcgataat
     4981 atagccaaaa caagtatcaa tatgccttgg tataaaggcg attcggtact taccatgctg
     5041 gataagttcc gggctacgaa acctttggat tacttacctt ttcgaatgcc ggtacaggat
     5101 gtatacaagt ttaccgccga taacgatgac agaagaatta ttgcgggaac ggtggaaagc
     5161 gggatactaa aacagggaga tcaagtggta ttctatccgt cgggaaagaa aacacatgtt
     5221 aaaagaatag agcctatgag cgagcaggaa gaagtcggaa aggcaagtgc cggatatgcc
     5281 acaggtttta ccatgaccga acagatatat gtaagaagag gggagttgtg cgtaaaagcg
     5341 gatgagttgc cgcctctcat gggaaagcac atgaaagtca gcctgttctg gctgggtaaa
     5401 aatcccatgg taaaagataa aaaatactac ctcaagaccg gtaccatgaa agcggaatgc
     5461 tatctggaag agatattgag cgttatggac gcatccactc ttgtgaaaac ggaaaaacaa
     5521 tatattgaca gacatgatgt agccgactgc attatctcta cctccagact gtgtgcattt
     5581 gacaatgttg cggctttggc atcgaccgga cggtttgtca tagtggatga ttatgagatt
     5641 tccggcggcg gtatatttac cgatacggtg gagaaccttc acaggtatga aagagccaaa
     5701 gtaatgttga gagaaggcaa atgggagcac ggttatgtga ccgttgagga aagaagcgac
     5761 aaatatgatc agaatcccgc cctcataata cttaccggag caaaagacac aaacaaaaag
     5821 ctgtttgcca aagcactgga aagaacgctt ttcgatgaag gcaggaatgt atattattta
     5881 ggtatcggaa gtgtgatata cgggctcagt gcggatataa atcctccggg agaaaaggaa
     5941 agcagatcgg gcgaaaatat ccgaagactg gccgaggttg ccaatatact taccgatacc
     6001 ggacttattc tcatagtaac cgcttccgat ctgaccaaat atgacataga aacgataaat
     6061 cttgtaacaa agaatgataa tatgctggta atctggctgg gcgaggaatg tactaccgat
     6121 atagagtgtg atatggtttt acgggccgat gaagatgcgt tggagattat aaggaagagg
     6181 gttgagcaga taactgcacg ttgattttat ggggataata gaaaacatta ggcaattcat
     6241 tttacagaca ccctccaatt tctgggtatt catactggta ggttttatcg cccaggttat
     6301 tgacggaaca ttgggaatgg catacggagt atcgtgtaat tcattcttac tcactttcgg
     6361 ggtgtctcct gcaatagctt cggcaagcgt gcatattgcc gaagtgttta ccacatcggt
     6421 ttcaggattt tctcattgga gactcaagaa tgtggataaa agtatgtttt tgaaactgct
     6481 tattcccggt gtgataggag gagccgtcgg tgcatatgtg ttatccaact ttgaaggcgg
     6541 aacaataaaa cccttcatca atgcctattt aatagtaatg ggtgttgtaa taatagttaa
     6601 gatattcaaa aagcagaaag aaaaacattt aggaggatgg atatatcctc ttggtcttgc
     6661 aggaggagta ctggatgcca taggcggagg gggctggggg ccggtagtaa cttccactct
     6721 catagcgacg ggtgtggaac cgcgctatac cattggaacg gttaatgccg cggagttttt
     6781 cgtgacactg gccgaatcaa tcatgtttat ttcttttttg ggcttgcaag acagcgtata
     6841 tgctatcttg ggattggtaa taggaggaat aatagcggca ccgttcggag catttttatg
     6901 taaaagagtg cctatgaagc ctttgatggc gcttgtaggt gccttggtaa tattcctcaa
     6961 cgtcaagtct ttgattgagt tttttattaa atgaggcgga tatgttcaag gattggaaaa
     7021 taggcgattt catattttat tttgcttcga taatactggt tgtagtatcg ggcattatct
     7081 ttaaagcata ctgggtatct tttctggtat cgaccgtggg aataaccgca ggcgtcatac
     7141 atatgaaagc caacaaatac agttatatac tgtacataat tcagataagt ctgtatgcat
     7201 atgtttcctg gcgcaacaga tttataggtg aagcggttct ggcggcattt tacctgttac
     7261 cgttatatat atattccgca atcaaatgga ttaagttagg caataaggaa aaaggcgatg
     7321 cccagatatt taagataacc aagaaacaga ttataatact tgccgttgca tgtgtagtgg
     7381 tgaccggagg ttacggttat gtgttgagtc ttctgaaatc caatcttccg tatgcaaacg
     7441 cattgggaac tttcttatcg gtggcagcgg ggtatctttc cgcaaagagg gtgaaagaac
     7501 aatggctttt ctggatatca tacagtttaa tcttttccgg aatatggata agcacattgg
     7561 gctccgatac atccaatgtt acattgatag tgcagaatat accctacctt tatattaata
     7621 tctccggcct tataaaatgg aacaaactgt acaaagaagc acatgcggaa gtttaacata
     7681 gtgcagattg attttcataa ttctataaca tttcatcttc tgtacaaatg cgatttttac
     7741 cttcaatgtt atcggctatc tttttggatt caagcattct caaaatactg tcaacatttg
     7801 attccgttac agtatgcgga tgctcttttt gaaattgaat aagaatgttt ttggtgagct
     7861 gttcctgctc ttttatctgc tttagtactt cttcatagtt atcagtcata acggcattcc
     7921 gaataaagtc ttttatttcc tttgcaatac cgttccagtc ttgatatagg ttaattactg
     7981 cctgattaac tttttcccaa gttaaaggtt cttctcgctt tttatgttct gccaagatac
     8041 ctatgatgtc ggacatatca ttcttatact ttcgtccgga cttacatttc attgcaatca
     8101 gatattctgc ggatatgcta cgcacttgaa gaatgccgta gaaggttttg taatacacgg
     8161 aaaattcatt gagtttctcg gaataggaag cggtatgcat aaagtcggta ttcaaccagc
     8221 cattgggcaa attatactca tcaccgattt ggttgattgc atcttttatg gaagctgaag
     8281 cgtgaatgat ggcatctata tctgcagtca tgtcccgaaa gccatagtta accaaaacag
     8341 ctgctccgcc cacaagaata atctctgcag tagctctttt accgttttgt ttccgaaaga
     8401 gtttagctaa cgcttttaag taagtatcca aattatcttt attgaatgca tgaactatat
     8461 catatgacat tgcgtacctc actttctata atattatgac gcaagaattc cggaacggat
     8521 tgagataatg catcttcctt aatcttagac tgtccgctta ttacagccat ggccagtatg
     8581 ccggcgggat aaagtgtttc ctccagtttt acattgcgta atgccttgta ttttgtgcac
     8641 agaggtatat tgtttacccg gcttacataa tcaagcatag caagcaggta gagactctcg
     8701 ggatactttt tacgatggta tagtttactg atattgtcat gctgaagggt ttctgcaatg
     8761 aaatctatat cgcccaattc ttttaatctg tgacaaacac tgcttttgta tagttcaaag
     8821 tcggttggtt cgcttaagta gggtattaga agttcctcca tactcacacc aagctcttta
     8881 gacagtttat acaccgtttc cgccgtacac ttgtccaggc cggttcttcc atgataaatg
     8941 tcgttaacgg tggtataagg aataccgctg ttttttgaca acctatacaa tgttatattc
     9001 ttgttttcaa ttagttccct aacagtcata tcagatgtct cctgctatgc attataacgg
     9061 cacaccgata taatgtcaat atactcggag taattgtttt tatgcaagta tgatttataa
     9121 aaataatatt ttgaattaaa gtcagtcaga aaattaatca aaaggttagg ttaaatattg
     9181 acaataaaac ataaaaagat aaaattccct ttaagcaggt atagctcaat ggcagagtcc
     9241 cggcttccca agccggttat gcgggttcga tccccgttac ctgctccata ttgaatgcat
     9301 aggtttgata catagttatc aggccttttt tctttggcag aaaggtaaga aaatcctatc
     9361 cgccagtttt gataagatca gtagtagtca gattttaggc tgtttactgg tcttcttttc
     9421 ttttagtaaa ctacttttga acgattactc tacttttgaa cgattagggc tagttttgaa
     9481 cgattactca acttttgaac gatttgagag aaacagggat aaaaaagtaa catatctttc
     9541 atttaatgga caaaatgata aaaaagtgat ataataacaa cataaagtac gttggaggta
     9601 aactctatga aatattctga agcaattaaa ttgttaagac ttaaaatgtg cctcactcaa
     9661 actggttggg ttagtgataa tttctcagct gctttagtaa agtttttctc ttctgcaaca
     9721 gcgattaatg tgaacaattt ttgatctaac atgatattca cctactatta gtaaaattta
     9781 tcgataccaa aataaatata attttacttt atgattggtt tgtgacgtaa ttatatcatg
     9841 ttatataaat gaatgaa
//
LOCUS       DAOSOM010000056         9820 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_6891_length_9820_cov_5.867691, whole genome shotgun sequence.
ACCESSION   DAOSOM010000056 DAOSOM010000000
VERSION     DAOSOM010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9820
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6891_length_9820_cov_5.867691"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            334..1734
                     /locus_tag="PLF14_09165"
     CDS             334..1734
                     /locus_tag="PLF14_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005427666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71489.1"
                     /translation="MKKMTFALCFSNRGFMPGELIYNAREDMVKAVTAAGYDYIMMDA
                     DLTRYGGVETRDEGRLYAKWLAEHNGRYDGVIFSMPIFADENGAIIALQDAGVPILMQ
                     AYPDEIGKMDFQHRRDAYCGKFSVTDVFCQYNVPFTVLKPHVVHPLSDKFRENLRDFA
                     AICRVVNGMKRFNLGCIGARTTAFKTVRFDEIALQKYGINVESFDLSELFAKVKDKKN
                     DDPAVSAKIQRLKNYSDFSLVPKENMYTLAKVSVVIDQYIKEYRLDALALRCWNEMET
                     YLRICPCVLLSELNDRGIVASCEIDVCSAVTMRAMSLASQGATAVLDWNNNYGEDENK
                     VILFHCGPVAQSLMTGRGTVTEHKMFSKSDPGSGWGSNEGRIKPMPITISNCQTKDGK
                     IIVYVSEGKITDDVIEREFFGCAGVAKIDDLQNKLIKLARGGFKHHTSICEGHYKEVL
                     KEAFTTYLHYDWVEID"
     gene            1747..3234
                     /locus_tag="PLF14_09170"
     CDS             1747..3234
                     /locus_tag="PLF14_09170"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOT71490.1"
                     /translation="MISLGIDIGGTGCKCVAFRDDGKQLAVAYTEYPLAAGQVNLPSD
                     ILTFSVFQVIKKCTAEIPDKQQIAAITVCSFGESFVPVDKDGNALTDILLYFSNTDSE
                     EFNNLVSSIGEEKFMRIARILPDASYSLSKMLYTRKVAKRPVWKYLFIANFITYRLTG
                     KAYTDVSLACRSLLYDVENRCWSKQLLELCSIEESTLPEVLPAGVKAGTILPKAAAQL
                     GLPCNVQVVTGCHDQIVNALGAGVRNAGDAVDSSGTSECITPLFSSIPESLDFQRNNF
                     ACVPYLEDRGYVTYAYNISAGSVVRWYRDALASHLNREAAMENKSIYDLLNEQCPSQP
                     SDLMMLPFLQGMGGTPDVDATATGLIAGLTTQTRLTDIYRAVLEGITFEMRYNREKLS
                     ESGVTFKKLFACGGGARSFVWLQIKADILGCEIIPVKSEETGAMGCAILGISAITGEK
                     PFDVAERFWQYGTPIRPNPEHMEIYNKKYKTYKTLRKLYMEQRSK"
     gene            3236..4213
                     /locus_tag="PLF14_09175"
     CDS             3236..4213
                     /locus_tag="PLF14_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005427664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HOT71491.1"
                     /translation="MKNTVNNYIGHASQLCGVEEVRLIGGKGDGIRLLQLRNAAGLQM
                     TVCADRCADIYRLIFKGDNMGYFSPAGYVAPAYYNQTGTGFLNSFTAGFLTTCGLTAA
                     GSPCTDEGEVLPLHGTIGNTPCEQIRWDEDDENIYIHAVINDSGIFSRKLMLKRTVTC
                     KKHINTFDIRDTIENLGDRQSPLMLLYHMNMGYPLLSENAVLDIPSVSVKPRNQHAAK
                     DLNTWHKILPPTPGFEEQCYFHSFRKQGKASIYNPDIGKGLTISFDANKLDYFTQWKM
                     MGCRDYVLGLEPGNCHPDGRDVMRREGTLKFIAPGESITYQVTVNLYEI"
     gene            4225..5220
                     /locus_tag="PLF14_09180"
     CDS             4225..5220
                     /locus_tag="PLF14_09180"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOT71492.1"
                     /translation="MPIITGRENVLAIYEKAAKKGWVIPCFCSENLTTTEAILTAASD
                     FAKEKGYDGIPVILAITNRYDRRTQSAYYTHTRRWDIGLELFRSDLEILSRVFPDLDV
                     MIHLDHAQHDTDEELLESDLSMYSSVMYDASVLPMEENMEKTRAYVRRKGQELLIEGA
                     CDEITDADGEIRCEITDADKCERYMRETGVDIVVANLGTEHRASGHDLHYRCDAARAI
                     KARIGSRICLHGTSSVSNDQIKKLFDDGICKVNIWTALERDSSPALTEWTVKNAAKCG
                     GPALEHKLIEQGYLTECSGTGNKSSLNYYTTTARQEIIFREMKKIVRGYLDLWYC"
     gene            5276..5791
                     /locus_tag="PLF14_09185"
     CDS             5276..5791
                     /locus_tag="PLF14_09185"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOT71493.1"
                     /translation="MKKICNRFKNNRWALISWILLIMWMVLIFILSSHTADRSSSVSG
                     NILSAILKVVYPEFRQMSADEQFIVLESYQYIIRKSAHFLLFGVLGSLSVNAYKSIRI
                     KTDRLVIILSLATCVINAILDEIHQIFVPGRSCEITDILIDSIGSLLFIFLTVYIFRC
                     IEKSKKASADI"
     gene            complement(5853..6917)
                     /gene="rpoD"
                     /locus_tag="PLF14_09190"
     CDS             complement(5853..6917)
                     /gene="rpoD"
                     /locus_tag="PLF14_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOT71494.1"
                     /translation="MVRFNKIMADLMEKGKTKGTLVSQDITDSFDDMEVNSDQMDKLI
                     EMFEEAGIDIVDDENDTSDADDETEDIEVSVPDGVSVDDPVRMYLKEIGKVPLLTVEE
                     EIELAKGIEAGDEECKRKLAEANLRLVVSIAKRYVGRGLQFLDLIQEGNLGLIKAVDK
                     FDYTKGFKFSTYATWWIRQAITRSIADQARTIRIPVHMVETINKYTRVSRQLVHELGR
                     DPTPEEIAKEMNMTEEKVREIMKINQDPVSLETPIGEEEDSHLGDFIPDEDALAPAEA
                     AAFTLLKEQLMSVLDTLTPREKKVLKLRFGLDDGRARTLEEVGKEFNVTRERIRQIEA
                     KALRKLRHPSRSKKLRDYLE"
     gene            complement(6997..8826)
                     /gene="dnaG"
                     /locus_tag="PLF14_09195"
     CDS             complement(6997..8826)
                     /gene="dnaG"
                     /locus_tag="PLF14_09195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOT71495.1"
                     /translation="MTERYIYPEPLIEEIISKNEIVDTVSRYVKIDRKGKDYFGLCPF
                     HQEKTPSFSVVPAKQIYYCFGCNKGGNVINFIKDIENLSYSEAVKLLAERANIVIPEK
                     NSYVDEAVLRKEKRTYEMNSLSARFFYENMRSEISNQAKEYLGKRGIKGETITLFGIG
                     YAPDEWSALTEYLKSKGFNDDEIVEAGLASKSKNKVFDKFRNRIMFPIIDIRNRVIGF
                     GGRALEEKDVKYMNSPETVVYKKNKSLYAMNLAKSAREDFVIIVEGYMDCISLHQAGF
                     TNVIASLGTSLTDNQAKMIKRVFGQAIIAYDNDAAGQNATLRGLDILAGLGCVVKVLM
                     LPSKDKFGNPIKDPDDFIKLMGREEFKEALKNALMLVEYKAHLLKQTCDLKTSGGKIA
                     FLKGVAAAIGQVQSPVERDVYIKNISARYNTSYDALLKEVQNRTGDTGRSDTSQTEIP
                     RTKKIASALTDRFTESKLDKLFYTILVLLCNNPSFKGEIEKQLLADNMIHNDFSRDLL
                     QEIINRYDSMESPDEAKIAAMLDDEQRAAFIKEAAEYHTISDPWKALNDYIAKYRHDM
                     IVNNNMVLSRALASDGLSESEKDNIKEELRKNTALLAKYKINT"
     gene            8934..9215
                     /locus_tag="PLF14_09200"
     CDS             8934..9215
                     /locus_tag="PLF14_09200"
                     /inference="COORDINATES: protein motif:HMM:NF039477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6514 family protein"
                     /protein_id="HOT71496.1"
                     /translation="MSTYTLRRSCRLIDERKNKLDVEYFLIEEFSLKEQCKTYGIKVE
                     TRENGKLTDTCSVSDVSVKQDEAIYILEKMADYEVTAVTARDVIEDMAL"
     gene            complement(9287..>9820)
                     /locus_tag="PLF14_09205"
     CDS             complement(9287..>9820)
                     /locus_tag="PLF14_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOT71497.1"
                     /translation="LNADTVRSYIDALKKMFVVEDAAAWNPNLRSSTAIRTSDTRYFV
                     DPSIATAALGLGPDDLLQDMSTLGFMFETLAIRDLRVYADASDGSVYHYRDKNGLECD
                     AVIHLRNGRYGLVEIKIGGDTLINQGAQSLLKLASLIDTEKMNKPSFLMVLTANSDMA
                     YKRPDGVYVVPVGNLGV"
BASE COUNT         2609 a         2320 c         2115 g         2776 t
ORIGIN      
        1 acccggcttc gccttccgga gcctgccgca acatgtggca gaagcgctcc cgctccctcg
       61 gataagccag aagttctctg aactccgcat caaaaataac gatacgaata tcggtcagcg
      121 tatagaaatc ctgcagcacc ttatacagat ttgcggtatt aagttgtagc atctctatac
      181 cacccttctt tttgcataat agcacaaaca ggataaacat acaatagcgg acaaatctac
      241 aatgttgcgg acaaataaaa ctattattga aatcactgat tatgtattct gaaatcagcc
      301 ataaaatgta ataatataag ggggatattt accatgaaaa aaatgacatt tgctctttgc
      361 ttcagcaacc gcgggtttat gcccggcgaa cttatttaca atgcccgtga ggatatggtt
      421 aaggctgtta ccgcagccgg ctacgactat attatgatgg atgcggatct gacgcgctat
      481 ggcggtgtgg agacccggga cgaaggccgc ctgtatgcaa aatggctggc ggagcataac
      541 ggccggtacg atggtgtcat attctccatg cccatttttg ctgacgaaaa cggcgctata
      601 atcgctctgc aggacgcggg agtacccatc cttatgcagg cttaccctga tgaaataggc
      661 aaaatggact tccagcaccg tcgtgacgct tattgcggta agttctcggt taccgatgtg
      721 ttctgccaat ataatgtgcc tttcacagtg ttaaaacctc atgttgttca ccctctttcc
      781 gacaaattcc gagagaacct ccgcgacttt gccgcaatct gccgtgtggt aaacggtatg
      841 aagcgattta acttgggttg cataggtgca cgtactaccg ctttcaagac cgtccgcttt
      901 gacgagattg ctcttcagaa gtacggtata aatgtggaat ccttcgacct ttccgaactg
      961 ttcgccaagg taaaggacaa gaagaacgac gaccctgccg tatcggccaa aatccaacgg
     1021 ctgaagaact actcggattt ttcccttgtt ccgaaggaaa atatgtacac actggccaag
     1081 gtgagtgttg ttatagacca atatattaaa gagtaccgtc tggatgctct ggctttacgt
     1141 tgctggaacg aaatggagac ctatctcagg atttgtccct gtgtcctgct cagcgaactg
     1201 aacgataggg gcatagtggc ttcctgtgag attgatgtgt gttccgcagt caccatgcgg
     1261 gcaatgagcc tggcttcaca aggtgctacc gctgttctgg actggaataa caattacgga
     1321 gaggacgaaa acaaggtcat actgttccac tgcggtcccg tagcccaaag cctgatgacc
     1381 ggtcgcggca ccgttaccga acataagatg ttttcaaaga gcgatcccgg aagcggctgg
     1441 ggcagcaacg aaggccgtat caagcccatg cccataacca tctccaactg tcagaccaaa
     1501 gacggtaaga ttattgttta tgtatccgaa ggcaagatta ccgatgatgt gattgaacgt
     1561 gagttcttcg gttgcgccgg ggttgcaaag attgacgatt tgcagaataa actgataaaa
     1621 ctggcccgcg gcggctttaa gcatcataca tccatttgcg aaggtcatta caaggaggtt
     1681 ctgaaggaag ccttcaccac ctatcttcac tacgattggg tggaaattga ctagaaggag
     1741 aataccatga taagtctcgg tatcgacata ggcggcaccg gctgtaaatg tgtagccttt
     1801 cgggatgacg gcaagcaact tgccgtcgcc tatacggaat atcccctggc ggcgggacag
     1861 gtcaacctgc cttcggatat cctgaccttt tcggtatttc aggtaatcaa aaaatgtacc
     1921 gctgaaattc ccgacaagca gcagattgcg gctatcaccg tctgctcctt cggtgaatcc
     1981 tttgtgcccg tagataagga cggcaatgca ttgaccgata tactgctgta tttcagcaat
     2041 acggatagcg aagaatttaa taatctggtc agcagtatcg gagaagagaa gtttatgcgt
     2101 atcgcacgta ttttacccga tgcatcctac tccctttcca aaatgctcta tacccggaag
     2161 gtagccaagc gacctgtctg gaaatacctg tttatagcaa acttcattac ataccggctg
     2221 acgggaaagg catacaccga tgtttccctg gcctgccgca gtttactcta tgatgtggaa
     2281 aaccgttgct ggtccaagca actcttggaa ctgtgcagca tcgaagaaag cacacttccc
     2341 gaagtgttgc ctgccggtgt aaaggcgggt acaatcctgc caaaagcagc cgcacaactg
     2401 gggcttcctt gcaatgttca ggtggtaacg ggttgccacg accagattgt caatgccttg
     2461 ggcgccggtg tacgcaatgc gggtgacgcg gtggattcct ccggcacctc cgagtgtatt
     2521 accccgcttt tttcctccat tccggaaagt ttggattttc agaggaataa ttttgcctgc
     2581 gttccttatt tagaggaccg cggatatgtc acttatgcat acaatatctc ggcaggctcg
     2641 gtagtacgct ggtatcggga tgcactggca agccacctga acagggaggc tgcgatggaa
     2701 aataaaagca tatatgactt actaaacgag caatgcccgt cacaaccttc agacctgatg
     2761 atgcttccct tcttacaggg aatgggcggc acacccgatg tggatgcgac tgccaccgga
     2821 cttattgccg gtcttaccac acagacccgt cttacggata tctaccgtgc agttttggaa
     2881 ggtataacct ttgagatgcg ctacaaccgg gaaaaactca gcgagagcgg agttacattc
     2941 aaaaaactct ttgcctgcgg cggcggtgca cggtcttttg tctggttgca aatcaaggcg
     3001 gatatcttag gctgtgagat tattccggtg aaatccgagg aaacgggcgc tatgggttgc
     3061 gctattctgg gcatttccgc cataacaggc gaaaagccgt ttgatgttgc cgaacgattt
     3121 tggcagtacg gtacaccaat ccgtcctaac cctgaacaca tggaaatcta caataaaaag
     3181 tacaaaacat acaaaacctt aagaaaattg tatatggaac aaaggagcaa atagtatgaa
     3241 aaataccgta aataattata taggacatgc aagccagctt tgcggtgtgg aggaagtacg
     3301 gcttatagga ggtaagggcg acggtataag gctgttgcag cttagaaatg ctgcaggctt
     3361 gcagatgacc gtatgtgccg accgctgtgc ggacatttac cggctgattt tcaaaggcga
     3421 taatatgggc tatttttctc ctgccggtta tgttgctccc gcttactaca accaaaccgg
     3481 cacgggcttt ttaaatagtt ttactgcagg atttcttacc acctgcggct tgacagcggc
     3541 gggaagtccc tgcaccgacg aaggcgaagt attacctctg cacggcacca taggtaacac
     3601 cccgtgtgag caaatccggt gggatgagga tgatgaaaac atatatatcc atgccgttat
     3661 aaacgacagc ggaatattct caaggaaact gatgctcaag cgtaccgtta cctgcaaaaa
     3721 gcatatcaac acattcgaca taagggacac aatagaaaac ttaggtgacc ggcaatcccc
     3781 ccttatgctg ctgtatcaca tgaatatggg atatcccctt ctgtcggaaa atgcggtact
     3841 ggatatccct tcggtgtccg taaagcccag aaaccaacat gcggcaaagg atttgaatac
     3901 ctggcataag atactgcctc ccactcccgg atttgaggag cagtgctatt tccacagttt
     3961 ccgaaagcaa ggcaaggcat ccatatacaa tcccgatatc ggcaaaggtc tgaccatatc
     4021 cttcgatgca aataaactgg actatttcac ccaatggaag atgatgggtt gccgtgatta
     4081 tgttctgggt ctggaaccgg gaaattgcca ccccgacggc cgcgatgtca tgagacgtga
     4141 gggtacattg aagtttattg ctcccggaga gagtattact taccaagtga ccgtaaacct
     4201 ttacgaaata taaggagaac agatatgcct attattaccg gccgggagaa tgtcttggca
     4261 atatatgaaa aagccgcgaa aaaaggatgg gtaattccct gtttttgcag cgaaaacctg
     4321 accaccaccg aagcaatact gaccgccgcc tccgacttcg caaaagaaaa aggatacgac
     4381 ggtattcccg ttattctggc cattaccaac cggtatgacc gacgtaccca gtccgcatac
     4441 tatacccaca ccagacgctg ggatataggg ctggagctgt tccgcagcga tctggaaata
     4501 ctatcccgtg tatttcccga cctggatgtg atgattcatc tggatcatgc acagcacgat
     4561 accgatgaag aattgctgga aagcgatctt agtatgtatt cctcggtcat gtatgacgca
     4621 tccgtactgc ctatggaaga gaatatggaa aagacacgtg cttatgtgcg acgcaagggg
     4681 caggagttat taatcgaggg tgcctgtgac gagataacgg atgccgacgg tgagatacga
     4741 tgtgaaatca ccgatgcgga taagtgcgag cggtatatgc gggaaacagg tgtggatata
     4801 gtggttgcaa acttaggcac cgaacaccgt gcctccggcc atgatctgca ctaccgctgc
     4861 gatgccgcaa gggccatcaa agcgagaata ggctccagaa tctgtctgca cggtacttct
     4921 tcggtttcca acgatcagat taagaaactg ttcgatgacg gtatatgtaa ggttaatatc
     4981 tggaccgctc tggagcggga ttcctcacct gccctcaccg agtggacagt caaaaatgca
     5041 gccaagtgcg gcggccctgc tttagaacat aagttaatag agcaaggcta tctgaccgag
     5101 tgctccggaa ccggtaacaa atcctctctg aattactaca caaccaccgc ccgtcaggaa
     5161 ataattttcc gggaaatgaa gaagattgtc cgcggttacc tggatttgtg gtactgctga
     5221 ttgattaata agcacagtta tagtattatg ctttggggtt gggaaaggat attacttgaa
     5281 aaagatatgt aaccgtttta agaataaccg atgggcatta atctcctgga tacttctgat
     5341 tatgtggatg gtattgattt tcatattgtc ttcccatact gccgaccgct cgtcttcggt
     5401 aagcggtaac attctttccg ctattcttaa ggtagtttat ccggaattcc gacaaatgag
     5461 cgccgatgaa cagtttatag tactggaaag ctatcagtat ataatacgca aatcggcaca
     5521 ctttctgtta ttcggagtct taggctcctt atcggtaaat gcatacaagt cgatacgtat
     5581 caaaaccgac cgccttgtga taatattgtc tttagcaaca tgtgtcatta atgcgatact
     5641 tgacgaaata catcagatat tcgttcccgg aaggtcctgc gaaatcaccg acattctaat
     5701 agacagcata ggctctttgc tgtttatctt tctgaccgtt tatatcttcc gctgtattga
     5761 aaaaagtaaa aaggcatcag ctgatatata aaaagcaatg aaaaagcggt aaaaacattt
     5821 taccgcaatc agtatttcat cgtaaaagta ttttattcca gataatctct cagtttctta
     5881 cttctgctcg ggtgtcgaag ttttctcaag gctttggcct caatctgcct tatacgctct
     5941 ctcgtgacat tgaactcctt gcccacttct tccagtgttc tggctctgcc gtcatccagt
     6001 ccgaaccgca gtttgagaac cttcttctct ctcggagtaa gtgtgtccaa taccgacata
     6061 agctgctctt ttaacagggt gaatgccgcc gcttccgcag gagcaagcgc atcttcatcg
     6121 ggaataaagt cgcccagatg actgtcttct tcttcaccta tcggggtttc caaagatacg
     6181 ggatcctgat taatcttcat tatttcacgg accttctctt cggtcatatt catttccttt
     6241 gctatttctt cgggggtggg atctcttccc agttcgtgca caagctgacg cgataccctt
     6301 gtgtatttgt ttatggtttc caccatatga acgggtattc ttatggttct tgcctggtct
     6361 gctatggatc gcgttattgc ctgacgaatc caccaggtgg catatgtact gaacttgaat
     6421 cctttggtat agtcaaactt atccaccgct tttataagtc ccaggttgcc ttcctgaatc
     6481 aggtccagaa actgcaagcc tctgcctaca tatctctttg ctatactgac caccaacctc
     6541 agattggctt ccgccagttt tctcttacac tcctcatcgc ccgcttctat acctttagca
     6601 agctctatct cttcttctac ggtcagcaac ggaactttac ctatctcctt caaatacatc
     6661 cttacggggt catcaaccga cactccgtcc ggaacactga cttcaatatc ttccgtttca
     6721 tcatccgcat ccgatgtgtc attttcgtca tctacgatat ctatacccgc ctcttcaaac
     6781 atctcaatca atttgtccat ctgatcggaa ttaacttcca tatcatcaaa actgtcggtt
     6841 atatcctggg atacaagcgt acctttggtt tttccttttt ccatcagatc ggccattatt
     6901 ttattgaatc tgaccattcc gctttcttct tccacaggat gctgttgcgc ctttttattg
     6961 gaattttctt tttcactcat ctgtactacc tccggattat gtatttatct tgtattttgc
     7021 caacagagcc gtgttctttc tcagctcttc tttaatattg tctttttcgc tctcacttaa
     7081 accgtccgat gccagtgcac ggcttaatac catattgttg ttcactatca tgtcatgacg
     7141 atatttggca atatagtcgt tcaatgcttt ccaaggatcg gaaatggtat gatattccgc
     7201 agcctctttt ataaacgcag ccctctgctc atcgtccaac atggccgcta tcttggcctc
     7261 atctgggctt tccatggaat catatctgtt aataatctcc tgaagaagat ccctgctgaa
     7321 gtcattgtgt atcatgttat cggcaagcaa ctgtttttca atctcccctt taaatgacgg
     7381 attgttgcaa agaagcacca gtatggtata gaagagttta tccagtttgc tttcggtaaa
     7441 cctgtcggtc aatgcggaag ctattttttt tgtcctcggt atctccgtct gcgaggtatc
     7501 ggacctgccg gtgtctcccg ttctgttctg tacttctttt aacagtgcat cataactggt
     7561 attgtatctg gcggatatgt ttttaatgta cacatcccgt tccacgggac tttgcacctg
     7621 tcctattgcc gccgcaacac cttttaaaaa tgcaatcttt cccccggaag tttttaaatc
     7681 acatgtctgt ttcagtaaat gtgctttgta ttccacaagc ataagagcat tcttcagcgc
     7741 ttctttaaac tcttctctac ccatcagttt aatgaaatca tcgggatctt ttatcggatt
     7801 accgaactta tcttttgagg gaagcataag tacttttacc acacaaccca gacctgccaa
     7861 tatatccagt cctcttaaag tggcgttctg cccggcagca tcgttgtcat atgctattat
     7921 tgcctgcccg aacactcttt ttatcatttt tgcctgatta tcggtaaggg aagttcccaa
     7981 tgaagctatg acattggtaa aacccgcctg atgaagactt atacaatcca tgtatccttc
     8041 cacaataata acaaaatcct ctctggcgct ctttgccaaa ttcatggcat acaggctttt
     8101 attcttcttg taaacgacgg tttccggtga gttcatgtac ttgacatcct tctcctccaa
     8161 tgctctgccg ccgaaaccta taaccctgtt tctgatatcg atgataggga acattatacg
     8221 gtttctgaat ttatcaaaca ccttgttttt tgactttgaa gccagaccgg cttccactat
     8281 ttcatcatcg ttaaatcctt ttgacttgag atattccgtc aaagccgacc attcgtcggg
     8341 agcatatcct atcccgaata atgtaattgt ttcgcccttt atgcctcttt tgcccagata
     8401 ttcctttgcc tgattgctta tttcagatct catgttttca taaaagaatc gggctgaaag
     8461 actgttcatc tcatatgttc ttttttcttt tctaagaacc gcttcatcca cataactgtt
     8521 tttttccggt atcactatgt tggctctttc agccaacagt tttaccgctt cggaataact
     8581 taagttttcg atgtctttta taaaattaat aacgtttccg cccttattgc agccgaaaca
     8641 gtagtatatc tgttttgcgg gaacaaccga gaaagaagga gtcttttcct ggtgaaacgg
     8701 acacagaccg aaatagtcct tgccttttct gtctattttc acatatctgg aaacggtatc
     8761 tacaatttcg tttttggaaa ttatctcttc tattaacggt tcgggataaa tgtatcgttc
     8821 ggtcattgcc cctcctgctt acattattcg acacatattg cttatttcct ttatttgtct
     8881 gttcaaaaat acatatttta ataatatcct aacgatatac ggaggtaaat aatatgtcaa
     8941 cctacacgct tagacgcagt tgcaggctta ttgacgaaag aaaaaacaaa ctggatgtcg
     9001 agtatttcct cattgaagag ttttctctga aggaacaatg caaaacttac ggcataaagg
     9061 tggaaacaag ggaaaacggt aaactcaccg atacctgttc ggtcagtgat gtatcggtta
     9121 aacaggatga agctatatac atactggaaa aaatggccga ttacgaagtt accgccgtta
     9181 cggcaagaga tgttattgag gatatggcac tatagcggaa aattaaccga atttacactg
     9241 cttttttgca ggtaaatatc caaagaaaaa agcatcatac aaaaaatcag actcccagat
     9301 ttcctacagg tacaacatat acgccgtccg gtcttttgta tgccatatcg gagtttgccg
     9361 tcaggaccat caggaaagaa ggtttattca ttttttccgt atcaatgaga cttgccagct
     9421 tcaataatga ttgagcacct tggtttataa gggtatctcc gcctatcttt atctcgacaa
     9481 gtccgtatct accgtttctt aaatgaataa cggcatcaca ttccagtccg tttttatcgc
     9541 gatagtgata taccgaaccg tccgatgcat ccgcatagac tcgtaaatcc ctgattgcca
     9601 aagtttcaaa cataaagccc aaagttgaca tatcttgcaa caaatcatcg ggacccaatc
     9661 ccaatgcggc tgtagcaatg gacggatcta caaagtatct ggtatccgat gttcttattg
     9721 cagttgagga acgtaaattg ggattccaag ccgcagcatc ttcaacaaca aacattttct
     9781 ttaatgcatc tatatatgag cgtaccgtat ccgcattcag
//
LOCUS       DAOSOM010000057         9757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_6953_length_9757_cov_6.732220, whole genome shotgun sequence.
ACCESSION   DAOSOM010000057 DAOSOM010000000
VERSION     DAOSOM010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9757
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6953_length_9757_cov_6.732220"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            589..1131
                     /gene="pyrR"
                     /locus_tag="PLF14_09210"
     CDS             589..1131
                     /gene="pyrR"
                     /locus_tag="PLF14_09210"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013158312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOT71498.1"
                     /translation="MEFKATLMDEDAVRRALIRISHEILEDNKGPEGICLVGIKTRGI
                     PLSERIAKNIENISSVKVPTGILDITLYRDDLSKYSEDPILNATDIQFEIKGKNVILV
                     DDVIFTGRTARAAMDAVFAIGRAAKIKLAVLIDRGHRELPIRPDFVGKNVPTAKDEMI
                     AVKLSETDGITGVCLYHIQM"
     gene            1161..2501
                     /locus_tag="PLF14_09215"
     CDS             1161..2501
                     /locus_tag="PLF14_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HOT71499.1"
                     /translation="MGKTKKEGFTLKTAILSFQHMFAMFGATILVPILTGMSISVALL
                     SAGLATIAFYFIAQKKVPVFLGSSFAFLPALISGMQGAGEIGSDTWNRAMGKVSVAVM
                     LAGLVYVIIALLLKRIGVDRIRKLFPPIVVGPVIVVIGMILAPKMFYNNIIGQAIWTG
                     IPAWKHWTAAGVTALTILLVSAVAKPKSFLKVIPILMGFVVGYIYSLIIGLVDYSNVD
                     WSQIIIFQDMGKTFGFYKYLTFDWGVILSLVPIAIVTFMEHLGDIAASSTVCGKDFMK
                     DPGVHRTLMGDGVGTMIAGAIGGPPNTTYGENTAVLAITKNYDPKNLFYAGIMAVIFG
                     SITIFGTAVGTIPSAVIGGASIVLFGMISAAGLRALVDGKVDFSDTKNILVVSVILSI
                     GLGLGAMSLAGDITGDSVYKIMIGTVEISPLAIATVAGIILNLIIPSDNREEKK"
     gene            2556..3740
                     /locus_tag="PLF14_09220"
     CDS             2556..3740
                     /locus_tag="PLF14_09220"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOT71500.1"
                     /translation="MMLLKNAYIFKDGSFHSSDLFIIDDKRWIAGKNLSAVKTDKVID
                     LQGKYIIPGLVDVHVHLRQPGFEYKETILTGSKSGAAGGFTSICAMPNVVPTPDSVDN
                     VRRIRKIIDSDACINVYQYGCITKKREGMELADIGKLSGYVIGFSDDGNPVSCDSIMA
                     AMLEQASLHDCLIATHCEDLSYEDPAKREYMHIERDIGLLRKTGISCRYHVCHVSRKE
                     SVELIRQAKKEGINITCETAPHYLIFTKDDIKSENYIMNPPLGNKEDREALLQAVCDG
                     TIDIIATDHAPHTKEDKAAGAMGVIGMETAFSSMYTYLVAAGCVSLERLVELMSVNPA
                     RIFGIPQYSDFTVLDLDKEYIVNTDEFFSMGRNTPFEGMRMKGRVIKTICKGKIVWEY
                     TQ"
     gene            3722..4447
                     /locus_tag="PLF14_09225"
     CDS             3722..4447
                     /locus_tag="PLF14_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOT71501.1"
                     /translation="MGIYTIESIRQLTYDTYEMTLSGDMSWIKAPGQFVNIKIDGFFL
                     RRPLSVCDIDDRYMTLIFKVAGEGTKALSMMKVSSQLDILCGLGNGYDINVSISEPLL
                     IGGGCGVPPLYYLAKTLKNKGITPSVVLGFNKKEDVFYEDKFKALGCNVCVCTVDGSY
                     GYIGLVTDIMDMFDYDYTFSCGPEGMLKAIYDKSIVSGQFSFEERMGCGFGACMGCTC
                     KTKYGYKRICKDGPVLFKEEILW"
     gene            4441..5355
                     /locus_tag="PLF14_09230"
     CDS             4441..5355
                     /locus_tag="PLF14_09230"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOT71502.1"
                     /translation="MVNLSVNLSGLTLSNPIIPASGCFGYGKEFSEYYDINILGSFCM
                     KGTTKEPRFGNETPRIAESSSGMLNSVGLQNPGIEHVVQQELKEIRAYYSKPVIANIS
                     GFSVEEYEYCVRRLRGIDNVGLIEVNISCPNVEQGGMSFGTDPEMAAKVTKAVVKVAD
                     KPVYVKLTPNVTDITEIAKACESEGASGICAINTLSAMRIDIKKQKPVLANITGGLSG
                     PAVFPVALRMVYQLYKEIKIPIIGIGGISKVEDVIEMMMAGATAVQIGSLNLVDPYIC
                     KTIINQLPGMLEQLGIDDINKIVGAAHR"
     gene            5352..6065
                     /gene="pyrF"
                     /locus_tag="PLF14_09235"
     CDS             5352..6065
                     /gene="pyrF"
                     /locus_tag="PLF14_09235"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOT71503.1"
                     /translation="MKKDVIVACDFPSRSEVMEFLQLFGKRKPFVKIGMELFYSEGPE
                     IVREIKKRGYKIFLDLKLHDIPNTVERAMRSLSMLDIDMVNLHTAGTIKMMQAALRGL
                     VRPDGTRPILIGVTQLTSTDEKELKEQLLVNGAMDDVVIAYAKLGVAAGIDGVVCSPL
                     EAGKVKEECGETFLTVTPGIRFKDSKKDDQARITTPYEASRMGCDYIVVGRPVTQATD
                     PVGAYERCIWEFVYGKESE"
     gene            6062..6691
                     /gene="pyrE"
                     /locus_tag="PLF14_09240"
     CDS             6062..6691
                     /gene="pyrE"
                     /locus_tag="PLF14_09240"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOT71504.1"
                     /translation="MKRAIAEHLLNIKAVFLSPDKPFVWASGIKSPIYCDNRITLSDH
                     FVREDIERGLAEIIKSNYPRCEAVMGTSTAGIAHAAITAHILKLPMGYVRSFAKDHGR
                     GNRIEGRLLPGQKVVVIEDLISTAGSVIDTVEALREEGAEVLGIASIFTYGMEKGLQR
                     LKEANITNVSLTDFDTIIDVACEKGFIKSEDKERLSAFRNNPADEGWIK"
     gene            6705..7625
                     /gene="pyrB"
                     /locus_tag="PLF14_09245"
     CDS             6705..7625
                     /gene="pyrB"
                     /locus_tag="PLF14_09245"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HOT71505.1"
                     /translation="MKHLMDINDLTLSQIDQLIATANDIIADKDKYKDVCKGKKLATL
                     FFEPSTRTRLSFEAAMIELGGSILGFSEAQSSSAAKGESVTDTVQTVGCYADIIAMRH
                     PKEGAPLAASKRTTVPIINAGDGGHSHPTQTLADILTIHREKGRLSNMTVGVCGDLKF
                     GRTVHSLINALSRYEGNEYICISPKELVIPDYVKANILEKNKIEYIETTDMEEYMPSL
                     DILYMTRVQRERFFNEADYVRLKDSYILTPDKLKSAKPDLSIMHPLPRVNEISVKVDD
                     DPRACYFKQVVNGKYMRMALILMLLGIEVK"
     gene            7634..8065
                     /locus_tag="PLF14_09250"
     CDS             7634..8065
                     /locus_tag="PLF14_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009215611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HOT71506.1"
                     /translation="MKIDSIVNGIVLDHIAAGNSLRIYKFLNLDDLECSVAVIRNCRS
                     KKYGRKDVIKIDSIIDMNLDVLGYIDPNITVNIIRDGKIAEKKHLELPERLVNVIKCH
                     NPRCITSIEQEVDHIFKLTDRENIIYRCAYCETKSDKEEIW"
     gene            8096..9028
                     /gene="dusB"
                     /locus_tag="PLF14_09255"
     CDS             8096..9028
                     /gene="dusB"
                     /locus_tag="PLF14_09255"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOT71507.1"
                     /translation="MIIQGKEITGLFLAPMAGITDAPFRTICIEHGAALTYTEMISAK
                     GLMYNNEKTKKLLSKMHPSEPLAVQIFGSETEPMKFATEYICSFNPVLIDINMGCPVS
                     KVVKNGEGSALMKDINKAYEVAKAVVNASSVPVSVKFRSGYDKQNLNYIEFGKAMQDA
                     GVACITLHPRTRDMFYSGKADWSHIVQLKQSVDIPVIANGDVFNEQDIEDIREYTGCH
                     DVMIARGSLGNPWIFSNSTPSLEQRVQVFRHHMALQIDYNGERKGIIEIRKHGAWYFK
                     GLKNSAKLRDLLFKAETKNDIINYIETILEIENA"
     gene            9199..9612
                     /locus_tag="PLF14_09260"
     CDS             9199..9612
                     /locus_tag="PLF14_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOT71508.1"
                     /translation="MLDLIRNKRNDYRVWDLMNDFFSTDVMTSVQGFRVDISEKDKEY
                     ILEAELPGFDKKDISVELKNNYLTISAKRDDDKEEKGKDFIRRERSYGEFSRSFYAKG
                     IKQEDIKAKYKDGVLVVSLPKKEKEEEVSTSIEIK"
BASE COUNT         3171 a         1600 c         2431 g         2555 t
ORIGIN      
        1 tgccgaacgg cacgtacggt ggtgtgagag ggcagttaat tctgccctac tcgatatttt
       61 aggcaatcat cggacttttt cggagcccga tttttgtttt tagagtgaaa aatgccgatg
      121 tgcactctgc ggatgaggtt gacgaaatac tgaataagga gttcggtatc tgattaagaa
      181 gtattctgta aactatttcg atgtaaccaa ctctgcttat tattgagtat ttaaaagctt
      241 tggtacagaa ttaatattaa aaagtctgta aagttaaaaa cactgtagtc ttatatgcat
      301 tcatgaaaat caaggattca atccctgaaa aacagggatg caatccttga aaatcataaa
      361 ttcctgttaa agacaggaac tttatccaaa cataagtgag gtattttttg cgtaaatttg
      421 tgcggcaaat tgctaaaacc ctcttgatat atttgagcat taatggtact atactccaag
      481 aactttaagt gcggtgcaga gatacccaca aggtgtttat aacgaacagg tttgtctatc
      541 cttgtatgca ttgcgcaaca gggatttttt ttagataagg agggggacgt ggagtttaaa
      601 gccactttga tggatgaaga tgcggtaaga agagcactta ttcgcatttc gcatgagata
      661 ctggaagata acaaaggtcc ggaaggtatc tgtcttgtag gcattaaaac aaggggaatc
      721 cctctgtcgg aaagaattgc aaagaatatt gagaatattt cctcggttaa agttccgaca
      781 ggtatattgg atattaccct ttatcgtgat gatttatcca aatattccga agatcctata
      841 ctcaatgcga cagatataca atttgaaata aaagggaaaa acgtcatcct cgtcgatgac
      901 gttattttta ccggtcgtac agcacgtgcc gcaatggatg ctgtttttgc tataggcaga
      961 gcggctaaaa ttaaattggc tgtgcttatt gacagggggc acagagagtt gcctataagg
     1021 ccggattttg tgggaaagaa tgttcctacg gccaaggacg agatgatagc agtaaaattg
     1081 tcggaaaccg acgggataac aggtgtatgc ctttatcata tacagatgta gcggaaataa
     1141 atcaggagga gaaaaaaact atgggaaaaa caaaaaaaga aggcttcaca ttaaagacgg
     1201 ctatactgag ttttcagcat atgttcgcaa tgttcggagc gacaatactc gtacctatct
     1261 tgacgggaat gagcatatcg gtagcactgc tttcggcggg attggcaaca atagcattct
     1321 actttatagc tcagaagaaa gttccggtat tcttaggaag cagctttgca ttccttccgg
     1381 ctctaatatc cggaatgcag ggtgccggtg agataggatc ggatacatgg aacagagcaa
     1441 tgggaaaagt aagcgttgcc gtaatgctgg caggtctggt ttatgtcata attgctcttt
     1501 tattgaagag aataggcgtt gacaggataa ggaaattatt cccgccgata gtggtaggtc
     1561 ccgttatagt ggttataggt atgattcttg cacccaagat gttctataac aacattatag
     1621 ggcaggctat ttggaccggg attcccgcct ggaaacactg gacggcagca ggagtcaccg
     1681 ctttgacaat tcttcttgta agtgcggttg caaaacccaa gagctttttg aaggtaatac
     1741 ctatactcat gggctttgtt gtcggatata tctattcgct cataatcggt cttgtggatt
     1801 attccaatgt cgactggagc cagattatca tattccagga tatgggtaaa acattcggct
     1861 tctacaagta cctgactttt gactgggggg taatactttc tctcgtacct attgcaatag
     1921 taacattcat ggagcatctg ggagatatag cggccagcag tacggtatgc ggcaaagact
     1981 tcatgaaaga tccgggagtg cacagaacat tgatggggga cggagtagga acaatgatag
     2041 ccggagccat cggaggacct ccgaacacta catacggtga aaatacagcg gtacttgcaa
     2101 ttacaaagaa ttatgatccc aagaatctat tctatgcagg cataatggct gtaatattcg
     2161 gctctataac tatattcgga acggcggtag gtactattcc ttccgcggta ataggaggtg
     2221 catccatagt attattcgga atgatttccg cagcaggctt gagagctttg gtagacggca
     2281 aagtggattt ttccgatacc aagaacattc tggtagtatc ggtaatactt tcaataggtc
     2341 tcggacttgg agcaatgagt cttgcaggtg atataacagg tgatagcgta tacaagataa
     2401 tgataggaac agtggaaatt tctcctctgg caatagcaac tgtagcaggt attatactta
     2461 accttataat tccttccgac aacagggaag agaagaaata agtgtaaatt acggctgccc
     2521 tctgaagaag ggggcagcct gcttaaaagg aggacatgat gcttttaaag aatgcttata
     2581 tatttaaaga cggaagtttt cattcctccg atttattcat tattgacgat aagcggtgga
     2641 tagcaggtaa aaatctttcc gctgttaaaa cggataaggt tattgattta caaggaaaat
     2701 atattattcc ggggttggtg gatgtgcatg tacatctgag acagccggga tttgagtata
     2761 aagaaacaat actgacagga tcaaaaagcg gggcagcggg aggatttacg tcgatatgtg
     2821 ctatgcccaa tgtggtgccg acaccggaca gtgttgataa tgtccgaaga attcggaaga
     2881 ttatagatag cgatgcatgt atcaatgtat atcagtacgg ttgtataact aaaaaaagag
     2941 aaggtatgga attggcggat atcggtaagt tgtccgggta tgtgataggt ttttccgatg
     3001 acggaaaccc cgtttcatgc gattcgatta tggcggcaat gctggaacag gcatcattgc
     3061 atgactgcct gatagctacc cattgcgagg acttgtctta tgaagatcct gcaaagagag
     3121 agtatatgca tatagagagg gatatagggt tgttgaggaa gacgggtata tcctgcaggt
     3181 atcatgtatg ccatgtgtca agaaaagaaa gtgttgaact tatccggcag gcaaaaaaag
     3241 aaggaataaa tattacatgt gagacggctc ctcattacct tatcttcact aaagacgata
     3301 ttaagtcgga aaactatata atgaatccgc ctttgggaaa taaagaagac agggaagctc
     3361 tgttgcaggc tgtctgtgac ggaacgatag atattatcgc caccgaccat gctcctcata
     3421 caaaggaaga caaagcagcg ggtgccatgg gagttatagg tatggaaacc gcttttagca
     3481 gtatgtatac gtatttggtg gctgcaggtt gtgtttcatt ggaacgactg gtggagctta
     3541 tgtccgttaa tcccgcccgt atattcggga taccgcaata ctccgatttt acggtactgg
     3601 atttggataa agaatatata gttaataccg atgagttttt ttcaatggga agaaatactc
     3661 catttgaagg catgagaatg aagggcagag ttattaaaac aatatgtaaa gggaagatag
     3721 tatgggaata tacacaatag aaagcataag gcagcttaca tatgatactt acgaaatgac
     3781 attaagcggt gatatgtctt ggataaaagc acccggacag ttcgtaaata taaagattga
     3841 cggattcttt ttaagaagac cgctgtcggt atgcgatatt gatgacagat atatgaccct
     3901 catattcaag gttgcaggag aaggcaccaa ggcactttcc atgatgaaag tttcttcgca
     3961 gctggatata ttatgcggtt tgggtaacgg gtatgatatt aatgtaagta tatccgaacc
     4021 tttattgata gggggaggtt gcggagtgcc gccgctatat tatcttgcaa agacccttaa
     4081 aaacaaaggc attacaccga gcgttgtttt aggtttcaat aaaaaggaag atgtttttta
     4141 tgaggataag tttaaggctt taggatgtaa tgtatgtgtg tgcaccgtgg acggaagtta
     4201 cggatatatc ggacttgtaa cagatattat ggatatgttt gattacgact atactttctc
     4261 ctgcggaccg gaaggtatgt taaaagcaat atatgacaaa agtattgtat cgggacagtt
     4321 ttcttttgaa gaaagaatgg gttgcggttt cggagcatgc atgggatgca cttgtaagac
     4381 aaaatacggg tataagagaa tatgcaagga cggaccggtt ttattcaaag aggagatatt
     4441 atggtgaact tatctgttaa tttaagcgga cttacattat ccaatcctat cataccggca
     4501 agcggatgtt tcggatacgg gaaggaattt agtgagtatt acgatattaa catattaggt
     4561 tctttttgta tgaagggaac gacaaaagag ccgagattcg gaaacgagac ccccagaatt
     4621 gcagaatcaa gtagcggcat gcttaacagt gtggggctgc agaatccggg aatagagcat
     4681 gttgtgcagc aggaacttaa agaaataagg gcgtattact ccaaaccggt gattgccaac
     4741 atttcggggt tttccgtaga ggaatatgag tactgcgtaa gacggttaag aggaatagat
     4801 aatgtcggac ttatagaggt gaatataagt tgtcccaatg tcgagcaggg agggatgagt
     4861 ttcggaaccg atccggaaat ggctgcaaaa gtgacaaagg ccgtggtgaa ggttgccgat
     4921 aaaccggtgt atgttaagtt aacacccaat gttaccgata ttacggaaat agccaaggca
     4981 tgcgaatcgg aaggagcgtc gggaatatgt gcaatcaata ccctttcggc aatgaggata
     5041 gacattaaaa aacaaaagcc cgtgcttgct aatataacgg gagggttgtc gggacctgcg
     5101 gtgtttccgg tggcactgcg tatggtttat cagttatata aagagattaa aataccgata
     5161 ataggcattg gcggaataag caaggttgag gatgttatag agatgatgat ggcgggagcg
     5221 actgcggtgc agataggttc tttgaatctt gtcgacccgt atatatgtaa gacaataatc
     5281 aatcagttgc ccggtatgct ggagcaactg ggtattgatg atataaataa gatagtagga
     5341 gcagctcaca gatgaaaaag gatgtaattg ttgcatgtga ttttccgtca agaagcgaag
     5401 taatggaatt tttgcaactg ttcggaaaga gaaaaccttt cgtaaagata gggatggaac
     5461 tgttctattc tgaagggccg gaaatagtga gggaaataaa gaaaagaggg tataaaatat
     5521 ttttagacct caaacttcat gatattccca ataccgtgga gagagccatg agaagcttat
     5581 cgatgctgga tatagacatg gtcaatctgc atacagccgg tacgataaag atgatgcagg
     5641 cggcgttgag aggtcttgta agaccggacg gaacccgacc gatattgata ggagtaaccc
     5701 agcttacttc caccgatgaa aaagagctta aagaacagtt gcttgtcaac ggagcaatgg
     5761 acgatgtggt aatagcttac gccaagctgg gagttgctgc cggaatagac ggagtggtct
     5821 gttcaccttt ggaagcaggt aaagtaaagg aagaatgcgg tgaaacattt ttaacggtga
     5881 caccgggaat ccggttcaaa gacagcaaga aagatgatca ggcacgcata accacacctt
     5941 atgaagcttc caggatggga tgcgactata ttgtagtcgg tagacccgta acgcaggcaa
     6001 ccgatccggt gggagcatat gaaagatgca tatgggaatt tgtttacgga aaggaaagcg
     6061 aatgaaaaga gcaatagcgg aacatttact taatatcaaa gcggtgtttt taagcccgga
     6121 taaacctttt gtctgggcaa gcggaattaa aagccccata tactgtgata acagaattac
     6181 gttatccgat cattttgtca gggaagatat tgaaagaggt cttgccgaga taataaaaag
     6241 caattaccca cgatgtgaag cagttatggg aaccagtacg gcgggtattg ctcatgcagc
     6301 cattaccgct catatattga aacttcccat gggttatgtg cgttcttttg caaaagacca
     6361 cggaagagga aaccgaattg aaggaaggct gcttcccgga cagaaagttg tggttatcga
     6421 agacctcata tccactgccg gaagtgttat cgatacggta gaggctttaa gagaagaggg
     6481 agcggaggtc ctgggtatag caagcatatt tacatacggt atggaaaaag ggcttcaaag
     6541 acttaaggaa gcgaatatca ccaatgtatc gttgacggat tttgatacga taattgatgt
     6601 cgcatgtgaa aagggattta taaagtcgga agacaaggaa aggttatcgg cattcaggaa
     6661 caatcctgcc gatgagggat ggataaagta aaggagactt tgatatgaag catttgatgg
     6721 atataaacga tcttacatta tcacagatag accaacttat tgctacggca aacgacatta
     6781 tagccgataa ggataagtat aaggatgtct gtaagggtaa aaaactggcg acattgtttt
     6841 ttgaacccag cacaaggacc aggctgagtt ttgaagcggc tatgattgaa ctgggaggta
     6901 gcatattggg attttccgag gcacaaagct cttcggcggc aaaaggagaa agtgttaccg
     6961 ataccgtaca gaccgtaggt tgttatgccg acataattgc catgaggcac cctaaggagg
     7021 gagctccttt ggcggcttca aaaaggacca cggttcccat aataaatgcg ggagacggcg
     7081 gtcacagcca tcccactcag acactggcgg atattttgac aatacatcgg gaaaagggaa
     7141 gactgtcaaa tatgacggtg ggagtttgcg gagatctgaa gttcggcaga accgtacatt
     7201 ctcttatcaa tgctctttcc agatatgaag ggaatgagta tatctgcata tcgccaaaag
     7261 aattggttat accggattat gtaaaggcaa atatattgga aaagaataaa attgaatata
     7321 tagagacgac ggatatggaa gaatatatgc cgtcgttgga tatattgtat atgacaagag
     7381 tgcagaggga gagattcttt aacgaagccg attatgtacg cttaaaagac agctatattc
     7441 tgactccgga caaattaaag agtgcaaaac ccgatttaag cataatgcat ccactaccga
     7501 gagtgaacga gatttcggtt aaagtggatg atgacccgag agcatgctac tttaaacagg
     7561 tggtgaacgg caagtatatg cgtatggcac ttatacttat gcttttggga atagaggtaa
     7621 agtgagggat gatatgaaaa tagacagcat tgtaaacgga atagttttag accacatagc
     7681 ggcgggcaac agtttgagga tatataagtt cctgaattta gacgacctgg aatgctccgt
     7741 tgcggttata cgaaactgca gaagcaaaaa gtacggtaga aaggatgtta tcaagataga
     7801 ctccattatc gacatgaatc tggatgtctt aggatatata gatcccaata ttaccgtcaa
     7861 cataataagg gacgggaaaa ttgccgagaa gaaacatctg gaacttcctg aaagactggt
     7921 caatgttatc aagtgccata atcctcgatg tattacctca atagaacagg aagtagacca
     7981 tatttttaaa cttaccgaca gagagaacat aatatacaga tgtgcatatt gtgaaaccaa
     8041 gtcggataag gaagagattt ggtaactgta gcagtaaatg aaaggataaa accttttgat
     8101 tatacaaggt aaagaaataa caggactgtt tttggcccct atggcgggaa taaccgatgc
     8161 gccgtttcgg acaatatgca tcgaacatgg agcagcgctt acatataccg aaatgataag
     8221 tgccaaaggt cttatgtata ataatgaaaa aacaaagaaa ctgttgagta agatgcatcc
     8281 gtccgagccg cttgcggtgc agatattcgg gtcggaaacc gaacctatga agtttgcgac
     8341 cgaatatatc tgttcattca atcccgtatt gatagatata aacatgggct gtcccgtatc
     8401 aaaggtggta aagaatggag agggcagcgc tttgatgaaa gatataaaca aggcatatga
     8461 agtggcaaaa gccgtcgtga atgcatcaag tgttccggtc agtgtcaagt ttcgttcggg
     8521 atatgataaa caaaatctta attacataga attcggtaag gccatgcagg atgcaggtgt
     8581 tgcatgtatt actttacatc cgagaactcg ggatatgttc tattccggta aagccgattg
     8641 gagccatatt gtacaattaa aacaatctgt ggatattccc gtaatagcca acggggatgt
     8701 tttcaatgag caggatatag aggatataag ggaatatacc ggttgccatg atgtgatgat
     8761 tgcaagagga agcctgggta atccgtggat attttcaaac agcacgcctt cattggagca
     8821 aagagtgcag gtattcagac accatatggc attacagata gactataacg gtgaaagaaa
     8881 gggaataata gaaatacgaa agcacggcgc ctggtatttc aaaggtctta aaaacagtgc
     8941 aaaactgaga gatctgctct ttaaagcaga gacaaaaaac gacattatta actatataga
     9001 aacaatttta gagatagaaa atgcttaatt attgagaata ataaagattc agcgagcaat
     9061 attgagatat cggaagatat ttcactttat ttccccctaa aggtcagata aagtcaaaaa
     9121 gaatacttga tacatgacga aagtatgata taatatatat aggtcaaaga aagtcaaagt
     9181 ttaatggagg tgacaagaat gttagactta attagaaata aaagaaacga ttacagagta
     9241 tgggatttga tgaatgactt tttcagcacc gatgtgatga caagtgttca gggattcaga
     9301 gtggatattt cagagaagga caaggaatat attcttgaag ctgaacttcc cggattcgat
     9361 aagaaagaca tatcggtaga gttgaagaat aactacctta caatttccgc taagagggat
     9421 gacgataaag aagaaaaggg aaaggatttc atcagaagag agagaagtta cggtgaattt
     9481 tccagaagct tctatgcaaa aggaattaag caggaagaca tcaaggcaaa atacaaggac
     9541 ggtgttcttg ttgtaagtct tcccaagaaa gagaaggaag aagaggtttc tacttctatt
     9601 gaaatcaagt agcttaatgg cttgataaat aggtgaaatt atccggcctt gccggatttt
     9661 ttcacgttaa gattacctca aaatgacgct gaagaataaa acaaaaaacc tgttgacagt
     9721 gcaatattgc tatgctaata tttaggagct gtccaaa
//
LOCUS       DAOSOM010000058         9635 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_7053_length_9635_cov_6.810230, whole genome shotgun sequence.
ACCESSION   DAOSOM010000058 DAOSOM010000000
VERSION     DAOSOM010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9635
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7053_length_9635_cov_6.810230"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="PLF14_09265"
     CDS             complement(<1..239)
                     /locus_tag="PLF14_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71509.1"
                     /translation="MKSNGDFGFFPGNNARANTTALQKAVDKTGTIRIEQPGIYDIDD
                     TVYIPSDTALVFGKGVYIRRTTDKGYVFINKGAYT"
     gene            complement(268..1164)
                     /locus_tag="PLF14_09270"
     CDS             complement(268..1164)
                     /locus_tag="PLF14_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOT71510.1"
                     /translation="MDNRKKTEKSEIFKPENVKRTVKDYALVTFGILLVAIGVHFFKF
                     PNNFAIGGVTGLAMILSKAFGGAISSATIVLVMNVILLIIAYLILGKDFGNKTLYGSL
                     LFSLVLSLLERIVPMQAPITDQPLLELAFAVIFPAFGQAILFNIGASSGGTDIVAMLL
                     KKFTHWDIGRSLFISDCILTLAGFAVFSVETVLLSILGLLIKSTLVDSVIENLNLNKY
                     FTIITEHPEEICDYILNHIKRSATVFDGKGAFTNEDKKIIITVMNRAQAVQLRQYIKQ
                     VDPGAFILITNTSEIIGKGFRA"
     gene            1262..2359
                     /locus_tag="PLF14_09275"
     CDS             1262..2359
                     /locus_tag="PLF14_09275"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT71511.1"
                     /translation="MDYRTRFEKTVKHIKTDRPPLDLNGTPLTSYHEEFLKAFIKYHN
                     MDALDVADAEEKIMRHYDIDFRRVGYIFKPPTVLTDNSKMSQGEFTNCWGIKWQLFGL
                     YWEIVHNPLKDATFEEIKAFPWPDAKDIDRNLIREETEKAKRLYYDTDYVVVAGHPVY
                     GYFELGCWMFGFDDFLYRIAAEPETAEWFFENYHKYVTDVNELYYGSLGKYIHVTTSG
                     DDFGMQNGPFISPDMFEEMIAPWYKKRIGQVKSMSNALFFHHSCGSVYRLLPAIIDMG
                     VDILNPIQPGTADMEPEKLKGEFGDKLVFWGGIDEQNLLSKATVEEVKKEVRRVSDIL
                     SENGGYILASSHNVQPDVPVENIDAMLTAFR"
     gene            2481..3023
                     /locus_tag="PLF14_09280"
     CDS             2481..3023
                     /locus_tag="PLF14_09280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT71512.1"
                     /translation="MFIFLSLIDNEQDKRKFEILYSLYYKRMFYVAKNILHDVHEAED
                     ALQNAFISLAANIDKVDDISSKSTLSFVLTVARNAAIDLLRKRDAIIYIDDDFFSHER
                     DIDISDIIVSEEEFNNVVAAIQKLPDKYKDVLNLHYINNFTVKETASLLSRKASTIKK
                     QITRGKKLLVKHLQEEGIME"
     gene            3020..3703
                     /locus_tag="PLF14_09285"
     CDS             3020..3703
                     /locus_tag="PLF14_09285"
                     /inference="COORDINATES: protein motif:HMM:NF025643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4367 domain-containing protein"
                     /protein_id="HOT71513.1"
                     /translation="MNKTKYTLRNAFIEAERIRLSEIPEDSDINWVPSSRFKKRIKRI
                     IKYSNNDSLFLLSNSFKKAVAIVAMIILLLGFSLNTAAAKQSLVRFVVTVYENYTNFF
                     FDVEEKEPDIPKTIEEKYTITAVPEEYMLSSTNEMEVLHQLIWTNEKGESILFSQYIL
                     QKVSLTLMTGDSPYKYIKIGTHTAFYSTKRNMQTVIWDNGKYLFTIKCTTSIPYKDLI
                     HIAESVAEK"
     gene            3810..5759
                     /locus_tag="PLF14_09290"
     CDS             3810..5759
                     /locus_tag="PLF14_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71514.1"
                     /translation="MKERKKSKWLLTIPAMLLVLLLSLCSALSCGIWCSAKNCITETL
                     EEYKSLAWLKESEFLPNSKYYTQLYPYCLLQKVHTLADSSSYADSVDIRFQCMAHAGL
                     NTVVSDECGVSVHSSVDKPYKDSVIVGTCEAIEFKSISQTEKSSGNISHFGYIATIKI
                     DKNLSPVLDPDYPYSYVRVMSSQIMADFSHFFVVGSKYIINGAVSYLHTPFKEDQAPY
                     FTMAETDFLPPVTEIKFEEYMYEKIGVPKGTKIITLHGRPRDLTYCYLAAYTDLSYLS
                     YAKIDNSLEEFLSSDENSTWNTIIADKKTAKESVKIVTANNIRNIPHFQSGRAYITQG
                     VPFTEEDYMSGKKICLISEQLAKSNDIKIGDVLELRFWNIGYELQTPPSGASWSPKTE
                     GCKSALSDSENYTVTGIYTDNNGWDTQCYDYTPNVVFVPELSVNDLVKAQPSLTVFDN
                     DKYTGIPDSRTYILKNGKASAFEAEMEALGYGGRFYYYEKDVSFLPYLYSLSDKSQSF
                     SYTFTVAFAVVVIVMAIYAFIRNILSTDKKPHMRAILTVIIIVAASILISAVICISFY
                     GSVIIATNEYITLETVNEQLLKADPFLSVPSAATMQVKLNVAPVSLLVIYAIQVGILL
                     LILGISSALCAISEKRMKRVRHLTD"
     gene            5960..>9635
                     /locus_tag="PLF14_09295"
     CDS             5960..>9635
                     /locus_tag="PLF14_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71515.1"
                     /translation="MKEKKKSKWLLRTSVMLLVFLLSLCSALSYGIWSSAKTCINKAM
                     DGKISLAWLKESEFLPNSKSATQAYPYSMLQEAYTAADSSLNVDSVDIRFQCMAHAGV
                     NTVVSDEYGVSVLASVDKPYKDAVIVGTCEAIEFKSTSKVTDSDGVSVPYFGYVITLK
                     IDKNLSPVLDSDYPYSYMRITSSQVMEDFSDFFIVGSKYIVIGTVSYLNTPFEEDQAP
                     LFNMASSDILLPTTDIKFEEHMYEKIGVPKGTKISYRRSYYDLTSCYLSAYTDSSFLS
                     YAKIENSLEEFLSSDENSRWNNINTSKKTAKESVKLVTVNNIESVPLFRSGRAYITQG
                     NSFTDKDYSSGNKVCLISEQLAKSNNIKVGDVLDMTLWNAGYDLQTGPLGASWSTKPY
                     SNKHALSEKEGYTVTGIYTDDDAWNTQCCAFTPNTVFVPDSCIGDSVKTYSSLTDLGS
                     DRYTGMPDSRTYILKTGKASDFEAEMEKAGYGGMFYYCENSAPSLPYLNSLSDTFRTV
                     TYISTSAFTFSILIIAIYAAVKNIEKTDKKKRLRTVLTVIIISVISIAVSAVISLSFY
                     GIVINAANKYISSETVTEQILKTEPTLPTSDIKTEQSIIDTAPTPLLMAFAVQTGILL
                     LVLCISSALCFVVKGEQKKKRIPYRLKKGDGIKGGMPAVLWYSIAQSARSTVMVLLMT
                     VLVTLSGIFMFLSVSTWITAKRSISEAENAYTTIAVAAENEFVKGISPFKSAIYDYSS
                     LAGIYETAEKSEHVKYVDIRDRCMAHSNAIKTISSGMVDEYTPIGAVDYPYDLSVIVG
                     TCVNVEYSKSGTATVSDDVLDGTVIMCMVTLELDRDQSFVLSEDFTFSRYMKVFAYVF
                     DNDLSDGFKVGDRYIVYGSKGYTQHPRNEMYRDSPVGIYPENLLRDSRYNRVADKYEY
                     VKFEPYMHKLLGTDSSTTIHTRDSKGQSIRMKIEDCYFRTYKDDAYMTYARLDGTLEQ
                     FLSSDKGQKWKKHIEECKTTVTSLKMNFTGNLNSVLLFNQNLAYITEGRTFTKEEYSE
                     GAKVCIISAETAKHSGIKVGDKIDFKFWSNGCNITKDYAQPIWVHNSFVDGCDFIDEG
                     SFEVIGIYKAENVWSTNEFFLTPNNAFAPKGSIKGQIEYNLPKYVAYSFDEKVGRPLI
                     SARGTYQSLPNTRSFVIKPGHIEAFEKEMESLGYGGMFYYYDQGFSAIASVLNSLKES
                     TALLMYISVAVWIVVMSAFFI"
BASE COUNT         3101 a         1856 c         1917 g         2761 t
ORIGIN      
        1 cttgtgtaag ctcctttatt gataaataca tatcctttat cggtagtcct tcttatatac
       61 acacccttac cgaataccag tgcggtatcg gaaggtatat agacggtatc atcgatatcg
      121 tatatgcccg gttgttctat tcttatagtt cctgttttgt cgaccgcttt ttgtaaagct
      181 gttgtgttgg ctcttgcatt attacccgga aagaacccga aatctccgtt gcttttcatt
      241 atatcctccg cactttatgt atggtaatca tgcccgaaaa cccttcccta taatctccga
      301 ggtattggtg ataagtataa aggctccggg atctacctgc ttaatatatt gtcttaactg
      361 caccgcctgt gctctattca taacagtgat gattattttc ttgtcctcat tggtaaatgc
      421 acctttcccg tcaaaaacgg tggcactcct ttttatgtga ttaagtatat agtcgcatat
      481 ctcttccgga tgctcggtta tgattgtaaa gtatttattc agattaagat tctctattac
      541 actgtcaaca agagtggatt ttatcagcag ccccaatatg gacaacagta ccgtctccac
      601 actgaaaacg gcaaatcccg ctaatgtaag aatacaatcg gaaataaaca gcgatctccc
      661 tatatcccag tgcgtaaatt tcttcagcaa cattgccaca atatcggtac cgccggaaga
      721 tgcccctata ttaaacaaaa ttgcctgacc gaatgcggga aatattacgg cgaaagccaa
      781 ctccaataaa ggctgatcgg ttatgggagc ttgcatgggt actattcttt ccaaaaggct
      841 taagaccaat gaaaaaagca ggcttccgta taaagtttta ttaccgaaat ctttccccaa
      901 tatcagatag gcaatgataa gcagaataac attcataacc agcactatag tcgccgaact
      961 tattgcaccg ccaaaagctt tactcagaat catggcaagt ccggtaactc cgcctatagc
     1021 aaagttattg ggaaatttga agaagtgaac acctattgcg acaagtaaaa tcccgaacgt
     1081 taccagggca taatctttta ctgttctttt cacattctca ggtttaaata tctcgctttt
     1141 ctctgttttt ttcctattat ccatcacaag taaattatca gcctaccggt gcaaatagtc
     1201 aatacataca tcggttaccg attacttgta aacaaagagg caatacaata taatcaaata
     1261 catggattac agaacgcgat ttgaaaagac agtaaaacac attaaaaccg acaggccgcc
     1321 gttggatctg aacggcactc ctttgacatc ttatcatgag gaatttctga aggcttttat
     1381 aaaataccac aacatggacg ctttggatgt tgccgatgcg gaagagaaga taatgcgtca
     1441 ttacgatatt gattttcgaa gagtgggata tattttcaag cctccgacgg tacttaccga
     1501 caattcaaaa atgtcacagg gagagtttac caactgctgg ggtataaaat ggcagttatt
     1561 cgggttatat tgggaaattg tgcataatcc cctgaaagat gcgacatttg aggaaataaa
     1621 ggcttttccc tggcctgatg ccaaagatat tgacagaaac cttatcaggg aagaaacgga
     1681 aaaagcaaaa agactgtatt acgataccga ctatgtggta gtagcgggac atcccgttta
     1741 cggctatttt gagctgggct gctggatgtt cggttttgat gattttctct acaggattgc
     1801 ggcggaaccg gaaacggccg agtggttttt tgaaaattat cacaaatacg ttactgatgt
     1861 gaatgaattg tattacggaa gcttgggaaa atatattcat gtaacaacca gcggagatga
     1921 tttcggaatg cagaacggtc cgtttatttc accggatatg tttgaagaaa tgatagcgcc
     1981 ttggtacaaa aagcgaatcg gtcaagtcaa atcaatgagc aatgccctct tcttccatca
     2041 ctcctgcggc tcggtataca ggctgttacc cgccataata gacatgggag tggatatact
     2101 gaatcccatt caaccgggaa ctgcagatat ggaaccggaa aagttaaaag gcgaattcgg
     2161 tgataaactg gttttctggg gaggcattga tgagcagaat cttttatcca aagccaccgt
     2221 tgaagaggtt aaaaaagaag tgcggagagt atcggatata ctaagtgaaa acggaggcta
     2281 tattttggct tcctctcata acgtacaacc ggatgtgcct gtagagaata tagatgcaat
     2341 gcttacggca ttcagataac acatgcctta ttttgccaat attgtcacta aaacggatat
     2401 atgttaatat gcatgtgtac ttttaagcga taatggagat aacttttcac gaaacaagag
     2461 tcttggaaca cggagaagta atgtttatat ttctaagcct gatagacaat gagcaggaca
     2521 aaagaaaatt cgaaattctg tatagcctat attataaaag aatgttttat gttgctaaga
     2581 acatcctaca tgatgtgcat gaagcggaag atgctctcca gaatgccttt atatccttag
     2641 ccgccaacat agataaggtt gatgatatta gttctaaaag tacactttct tttgttttga
     2701 ctgttgcaag aaatgcggct attgacctgc taaggaagag agatgctata atttatattg
     2761 atgatgactt tttttcacat gaacgcgaca ttgatatctc cgatataatt gtatctgaag
     2821 aagaattcaa caatgttgtc gctgcaatac aaaaactgcc tgacaaatac aaagatgtac
     2881 ttaatttgca ttatataaac aactttaccg tcaaggaaac agcatcattg ttgtcgagaa
     2941 aggcgtcaac aataaaaaaa cagattacaa gaggtaagaa actgctcgtg aagcatttac
     3001 aggaagaagg tatcatggaa tgaacaagac aaaatataca ttacgcaatg cctttataga
     3061 agcggaaaga attcgattat ccgaaatacc cgaagattcg gacataaatt gggttccgtc
     3121 ttcgaggttt aaaaaaagga taaaaagaat tataaaatac agcaataatg attctttgtt
     3181 tttgctcagc aactcgttca aaaaagctgt cgctattgtt gcgatgataa tactgttatt
     3241 aggtttctca ttaaacacgg cagcggcaaa acaatcttta gtccggtttg ttgttacggt
     3301 atatgagaat tatacaaatt ttttctttga tgttgaagaa aaggaacctg atattccgaa
     3361 aacaattgaa gaaaagtata cgataactgc cgtaccggaa gaatatatgc tttcttctac
     3421 aaatgaaatg gaagttctgc accaattgat ttggacaaac gaaaaaggcg aaagcatact
     3481 cttcagccaa tatattctgc aaaaagtcag tttaactttg atgaccggag actctccata
     3541 taaatatata aagattggga cgcatacagc tttttactca acaaagagaa atatgcagac
     3601 tgttatatgg gataacggta aatacctttt cactataaag tgcacaactt ctattccata
     3661 taaagactta atacatattg cagaaagtgt agctgaaaaa tgataactct tttaaaaatg
     3721 tttgcaaaat ccgtacttgt ccttcctctt ctttttacta tatataatgt agggcaaaac
     3781 tgcagacaac aaggtaacgg aggtaataaa tgaaagaaag aaagaaaagc aagtggttat
     3841 taacaatacc tgccatgctg cttgttcttt tactaagctt atgttcggct ctttcctgcg
     3901 gaatatggtg ttctgcaaaa aattgtataa ccgagactct ggaggaatat aaatcgttag
     3961 cttggttaaa ggaaagcgaa tttcttccta acagtaaata ttacacccaa ctctacccgt
     4021 actgcctgct acaaaaagta catactctcg ctgactcatc ctcatatgca gattcagttg
     4081 atattcggtt tcaatgcatg gctcatgcag gactgaatac cgtagtgtcc gatgaatgcg
     4141 gcgtttccgt tcattcctct gttgataaac catacaagga ctctgtaata gtaggaacat
     4201 gtgaagcgat agaattcaag agtatatcgc agacagaaaa aagctccggc aatatatcgc
     4261 atttcggata tatcgctacc attaaaatag ataaaaactt atctcctgta cttgatcccg
     4321 actatcctta ctcgtatgta agggtaatgt cttcgcagat aatggcggac ttcagccact
     4381 tctttgttgt cgggtctaaa tacataatta acggggctgt ttcctatctc catacccctt
     4441 tcaaggaaga tcaggctccg tactttacca tggcggaaac ggatttttta cctccggtga
     4501 ccgaaataaa gtttgaggaa tatatgtatg aaaagattgg cgtgccgaaa ggaacgaaga
     4561 taatcacatt gcatggcaga cctcgtgatc ttacatattg ttatttagcc gcatatactg
     4621 atttgtcata tctttcttat gcaaaaatag ataattcttt ggaagagttc ctttcttccg
     4681 atgagaatag cacatggaac acaataatag cagacaaaaa aacggcaaag gaatccgtaa
     4741 agatagtaac cgccaacaat attagaaaca ttcctcattt tcaaagcggc agagcatata
     4801 ttacacaagg agtccctttt accgaagaag attatatgtc cggtaaaaag atttgcctta
     4861 ttagtgaaca acttgcaaag agcaacgata ttaagatagg ggacgtcttg gaactgagat
     4921 tctggaatat aggatatgaa cttcagacac ctccctcggg agcatcctgg tccccgaaaa
     4981 ctgaaggatg taagagtgcc ttgtcggaca gtgaaaatta taccgttacg ggcatctata
     5041 cagataataa cggatgggac acgcaatgct acgactatac ccctaatgtc gtttttgtac
     5101 ctgaactatc agtaaacgat ttagtaaagg cacaaccttc actgaccgtt ttcgataacg
     5161 ataaatatac cggtatacct gattcaagga catatattct taaaaacggg aaagcatcag
     5221 cctttgaggc cgagatggaa gcgttgggat atggaggaag gttctactat tacgaaaagg
     5281 atgtttcttt tcttccttat ctatattctt tatcagacaa atcccaaagc ttctcctata
     5341 cctttaccgt tgcttttgcg gtagttgtca ttgtcatggc tatatatgct ttcataagaa
     5401 acatcctaag taccgataaa aagccgcata tgcgtgcaat tcttactgtc atcataatag
     5461 tagccgcttc catccttata agtgcagtta tatgcatttc tttctacggc agtgttatca
     5521 tcgccacaaa tgaatatata acattagaaa cagtaaacga acaattattg aaggccgatc
     5581 cttttctttc ggttccgtca gctgcgacta tgcaggtaaa acttaatgtt gctcctgtct
     5641 cacttcttgt aatatacgcc atccaggtcg gaatacttct gctcattctc ggcatatcat
     5701 ctgcattatg tgccataagt gaaaaaagga tgaagagagt aagacacctt acagattaag
     5761 tcaagataca ataataaaag ataaaagaaa aggtaaatga gtagagaact caaatacttt
     5821 cacacaaaat atattcacta aaaacaactg aaattataac tttctcaaaa agtttctgat
     5881 ttttgtccac ccccggtcct tttttttcgt tatatatagt gtaaagccaa ctaaaaaaca
     5941 aatcatcggg aggcattaag tgaaagaaaa aaagaaaagc aaatggctat taaggacatc
     6001 cgttatgttg cttgtttttt tgttaagctt atgttccgct ctttcttacg ggatatggag
     6061 ctcagcgaaa acctgcataa acaaagctat ggacggaaaa atatctttag cctggctgaa
     6121 agaaagcgag ttcctgccta acagtaaatc ggctacccaa gcttatccgt acagcatgct
     6181 gcaggaagca tatactgctg ccgacagttc attgaatgta gattcggtcg atatcaggtt
     6241 tcaatgcatg gctcatgccg gagtgaatac cgtggtatcc gatgagtacg gtgtttccgt
     6301 tcttgcatct gtagacaagc cgtacaagga tgccgtaatt gtgggaactt gcgaagctat
     6361 agaattcaaa agcacatcaa aagtaaccga cagtgacggc gtgtcggtac cgtatttcgg
     6421 ttatgttatt acacttaaga tagataaaaa cttatctcct gttcttgatt ccgactaccc
     6481 ttattcatac atgaggataa catcttctca ggttatggag gatttcagcg atttctttat
     6541 tgtcggctct aagtatatag tcataggaac ggtatcctat ctcaacacgc cttttgaaga
     6601 agatcaggct cctcttttta atatggcaag ttcggatata ctgttaccta caaccgacat
     6661 caagtttgaa gaacatatgt atgaaaagat aggtgtacct aagggaacaa agatatctta
     6721 cagacgctcc tattatgacc ttacatcatg ctatctttcc gcatataccg attcttcatt
     6781 tctttcttat gcaaaaatag aaaattcttt ggaagagttc ctttcttccg atgaaaacag
     6841 cagatggaat aatatcaata caagtaagaa gacggcaaag gaatcggtaa aattagtcac
     6901 agtaaataac attgaaagtg ttcctctttt cagaagcggc agagcatata ttacacaggg
     6961 aaactctttt accgacaaag actattcttc cggtaacaag gtttgtctca taagcgaaca
     7021 gcttgcaaag agtaataata tcaaagtcgg ggatgttttg gatatgacat tatggaatgc
     7081 cggatatgac cttcagacag gtcctttagg agcatcgtgg tcaaccaaac cgtatagcaa
     7141 caaacatgca ttatcggaaa aagagggtta tacggttacg ggcatctata ccgatgatga
     7201 cgcatggaat acacaatgct gcgcctttac ccccaatacc gtatttgtac cggattcttg
     7261 tattggtgat tctgtaaaga catattcttc actgaccgat ttgggcagtg acagatatac
     7321 cggcatgccc gattcaagga catatattct caaaacaggt aaggcatccg attttgaggc
     7381 tgagatggaa aaagccggat acggaggaat gttctattac tgtgaaaaca gcgctccttc
     7441 cttaccttat ctcaattccc tgtcggatac attccgaaca gtaacatata tttccacatc
     7501 tgcttttaca ttttcaattt tgattatagc catatatgcc gctgttaaaa acatagaaaa
     7561 aaccgataag aaaaaacgac tgcgtacggt tcttacggtt atcataatct ctgttatttc
     7621 tattgcagta agtgcggtta tatctctttc tttttacggt attgtgatta atgctgcgaa
     7681 taaatatatt tcatcggaaa cagttaccga acaaatctta aagaccgaac ctacactgcc
     7741 tacttccgac ataaaaacgg aacaatcgat aattgacacc gctcctactc ctttacttat
     7801 ggcatttgcc gttcagacgg gaatacttct tcttgttctt tgcatatcgt cagcattatg
     7861 ctttgttgta aaaggtgaac aaaagaagaa aagaataccg tacagattaa aaaaaggtga
     7921 tggaataaaa ggcggtatgc ctgccgtact gtggtacagt attgcacagt ccgcaaggtc
     7981 cactgtaatg gtacttctta tgacagtgct tgtaacactc tccggcatat ttatgttctt
     8041 aagtgtaagc acatggataa cggcaaaaag aagcatatcc gaagcggaaa atgcatacac
     8101 gactattgcc gtagctgcgg aaaacgaatt tgtaaaagga ataagtcctt tcaaaagtgc
     8161 gatatatgat tacagttcac ttgcaggtat atatgaaact gcggagaaat ccgagcatgt
     8221 aaaatatgtc gatattcgcg atagatgcat ggcgcatagt aatgctataa aaacgatatc
     8281 gtcgggaatg gtagacgaat atactcccat aggcgctgta gattacccct atgatctttc
     8341 cgttatagta ggcacatgtg taaatgtaga gtatagcaaa tccggtacgg caaccgtttc
     8401 cgatgatgtt ttagacggta ctgtaattat gtgtatggta acattggagc tggaccgtga
     8461 ccaatctttc gtactcagcg aggactttac attcagcaga tatatgaaag tttttgcata
     8521 tgtctttgac aacgatctca gtgacggatt caaggtcggc gatagataca ttgtctacgg
     8581 ctcaaaaggc tacacacagc accccagaaa cgaaatgtat agggatagcc ctgtaggcat
     8641 ttatcctgaa aacctgttgc gtgacagcag gtataatcgt gttgcggata aatatgaata
     8701 tgttaagttt gagccctaca tgcataaatt attaggaacc gacagcagca ctaccataca
     8761 cacacgtgat tctaaaggac aaagtatcag aatgaaaata gaggactgct attttcgcac
     8821 atacaaagat gacgcataca tgacatatgc aaggcttgac ggtacactcg aacagttttt
     8881 gtcttccgat aaagggcaaa aatggaagaa gcatatagag gaatgtaaaa caacagtaac
     8941 ttcactcaag atgaatttta cagggaatct taacagtgta ctgcttttca accagaatct
     9001 tgcatacatc accgagggta ggacctttac aaaagaagaa tactcggagg gagcaaaggt
     9061 atgcatcata agtgcagaga ccgcaaagca cagcggtata aaagtgggag ataaaataga
     9121 tttcaaattc tggtcaaacg gctgtaatat tactaaggat tacgctcaac ctatatgggt
     9181 acataattcc ttcgttgacg gatgtgactt cattgatgaa ggatcttttg aagtcatagg
     9241 catatacaag gccgagaatg tatggagtac aaacgagttc tttttaaccc ccaataacgc
     9301 ttttgcacct aaagggtcaa taaagggaca aattgagtat aaccttccca aatatgtagc
     9361 ttatagtttt gatgagaaag tgggcagacc tcttatttcc gcacgcggta cctatcaaag
     9421 ccttcccaat acccgctcgt ttgtcattaa gcccggtcat attgaggctt ttgaaaaaga
     9481 gatggaatcg ttgggatacg gaggaatgtt ctactactat gaccaggggt tttcagcgat
     9541 agcatcggtt cttaactctc ttaaggaaag cacagcactt cttatgtata tatccgttgc
     9601 tgtatggata gtggtaatgt cggcattctt tatca
//
LOCUS       DAOSOM010000059         8871 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_7853_length_8871_cov_6.558643, whole genome shotgun sequence.
ACCESSION   DAOSOM010000059 DAOSOM010000000
VERSION     DAOSOM010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8871
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7853_length_8871_cov_6.558643"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..1411
                     /locus_tag="PLF14_09300"
     CDS             125..1411
                     /locus_tag="PLF14_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT71516.1"
                     /translation="MTAIYRNSNKNKHLTLEERIIIERSIEKGSTKTAIAKVLGKDNS
                     TVGKEIAQHRTLTYPCALPLECAVYKKCKHGRLCHKDCPDYRPFYCPRRDRSPGACNG
                     CPTYANCRFSKYRYEAYKAQANYKEVLVGTRKGLDLDKDDVDRLASIVVPAIKKGLSP
                     YHIVHMYPDIGISEKTLYNYIAQGVFRPWNVIDLDLRQKVKRKQPKKETVTYKKREDK
                     KHLKGRLYTDYLQYISEEPDLSTVQMDTIYNRIEGPFIQTFHFLEYDFSFGFFHEKRT
                     AENMVKGINLLEDIIGKDLFIQHIRILLTDRGGEFSNPEAMEKDSDGLTRTRIFYCDP
                     QAAWQKGALEKRHTEWRYILPKEIGLRELGLLGQDDLNLVLSHLNSVPREKTRGKSPF
                     ELLQFFCPELLGAFTDFGIHEIPKDEVILMPYLLKK"
     gene            complement(1784..1858)
                     /locus_tag="PLF14_09305"
     tRNA            complement(1784..1858)
                     /locus_tag="PLF14_09305"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1823..1825),aa:Glu,seq:ctc)
     gene            complement(1997..4450)
                     /locus_tag="PLF14_09310"
     CDS             complement(1997..4450)
                     /locus_tag="PLF14_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HOT71517.1"
                     /translation="MSKTVIIGGVAGGASCAARLRRLDENAKIVILERGEYISYANCG
                     LPYHIGDVIKSRKALFLQTPENMRQRFRLDVRVKNEVIAIDRKKKSVSVKEIDTGRIY
                     QESYDTLVISTGSSPLRPPIPGIDSPRIKTLWTVPDMDDIKAMIQQQNVKTAAVIGGG
                     FIGLEMAENLQHAGLEVSVMEMQDQVMAPLDYEMAQLLHENITGNGVNLYLADGVTSF
                     ADKDDKVIITLSSGKTASADLVILSIGVRPNSKLAKEAGLKLNARGGIVTDEYLRTSD
                     PDIYAVGDVIEVEDYISKERTMVPLAGPANKQGRIAADNIAGGSKTYKGTQGTSVAKI
                     FDLTAASTGANEKTLIKQGLKKGKDYETVLIMQNSHASYYPGAVPMTLKLLFSADGKK
                     LFGAQIVGADGVDKRIDVIATAIRLGGGVSQLKELELAYAPPYSSAKDPVNMAGFVAE
                     NIMKGIVRFSEWNAVENNPDAVLLDVREEAEIMAFALPGAKHIPLGQLRDRINELDKN
                     KRTIVFCAIGVRSYNATRILMQNGFTDVLLYPAGTRFYRSTHYDKYDNVKRNALPVSD
                     SGDIKKYISSTASISIDCTGLQCPGPIMKVFETLQDMKDGEILEVTASDPGFMKDIGA
                     WCRRTGNTLLTNEKKGNTYVATVKKGSNSDKTVVTKEADGKTIIVFSGDLDKVLASFI
                     IANGAAAMGRPVTMFFTFWGLTALRKAKKQPVKKTFMEAMFGRMLPRGSKKLSLSKMN
                     MGGMGTAMMKKIMKDKNVDSLETLIEKAMKQGVKIVACTMSMDVMGIKPEELIDGVEL
                     AGVGSYLGDAEESDVNLFI"
     gene            complement(4443..4685)
                     /locus_tag="PLF14_09315"
     CDS             complement(4443..4685)
                     /locus_tag="PLF14_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HOT71518.1"
                     /translation="MKYKFRDILKRYLPVIIAAIIGAGAGYLYYLFVGCASGTCVITS
                     NPVISTLYFALLGGLLGRIISDRSCKRKNRKEEEHE"
     gene            complement(4833..4909)
                     /locus_tag="PLF14_09320"
     tRNA            complement(4833..4909)
                     /locus_tag="PLF14_09320"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4873..4875),aa:Met,seq:cat)
     gene            4995..5939
                     /locus_tag="PLF14_09325"
     CDS             4995..5939
                     /locus_tag="PLF14_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007956079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HOT71519.1"
                     /translation="MLYPLKFEPVYKDYLWGGNNLTKLNKKLIYPITAESWEISANKD
                     GMSIVSNGNLAGSTLLDIFQRYPKKLCGKREVKQFPLLIKLLDACSALSVQVHPDDAY
                     AYANENGALGKNEMWYVVDAKPDARLVYGLKKGVTKEQFAKAVRQNNIGSTLNYVSVK
                     KGDWINIPAGLVHAIGAGIIIIEIQQNSNTTYRVYDFDRVGADGKKRPLHIEKALDVI
                     NFDATEMQINEIVSVSGVDYLAYNKYFAVEYIASEGQYKSDTLNLSFHTYTVIEGGFE
                     VEGVSVGLGESVLIPACMKKYTLKGGFKAIKSFVPDIK"
     gene            6114..6395
                     /gene="rpsF"
                     /locus_tag="PLF14_09330"
     CDS             6114..6395
                     /gene="rpsF"
                     /locus_tag="PLF14_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOT71520.1"
                     /translation="MNQYETIFIVDCTMEEEVQKSIVEKMVQQMTDAGCVVEKVDEWG
                     RRKLAYPINYKTDGYYVLVTFKAAPNFISELERLYRITEGILKGQTIRL"
     gene            6405..6815
                     /locus_tag="PLF14_09335"
     CDS             6405..6815
                     /locus_tag="PLF14_09335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOT71521.1"
                     /translation="MNKVILMGRLTRDPELRTTATGTPVASFSLAVNRRFAAKDAQVQ
                     ADFFNVTAWSKQGEFVNNYYRKGMQVVVIGRLQNRTWEDQEGKKRYATDVILEETYFA
                     EGKRDSGVSSTGSNAGEDMDFTHIDISEDDALPF"
     gene            6846..7100
                     /gene="rpsR"
                     /locus_tag="PLF14_09340"
     CDS             6846..7100
                     /gene="rpsR"
                     /locus_tag="PLF14_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOT71522.1"
                     /translation="MDRMDKEDRKDFKFRRPKKKICQFCADKNTIDYKDLNTVKRYIS
                     ERGKILPRRVTGTCAKHQRDVTIAVKRARQIALLPYVTDN"
     gene            7228..8013
                     /locus_tag="PLF14_09345"
     CDS             7228..8013
                     /locus_tag="PLF14_09345"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HOT71523.1"
                     /translation="MNILIMTDLEGISGIYASDQVLPGERRFNEGREFMTADINACAR
                     GLKRAGAKKVYVRDCHGGSYTLIQERISEDVDYLILGDVGQDRFLGLDDCQAVILLGY
                     HAMAGTSSAILEHTMSSSYVQNYWINGKKAGEFAIDAGIAGDMGKPVIMVSGDDKLCS
                     EAKSLVPNIYCAEVKKGVTAFGGVLLPLEKARKLIEDTAYEAYKNMDKIKPLVFKKPI
                     RLRVERVERGRLPLLASKPYMKIIDARTYEVKADTMNEALFRI"
     gene            8152..8415
                     /gene="rpsO"
                     /locus_tag="PLF14_09350"
     CDS             8152..8415
                     /gene="rpsO"
                     /locus_tag="PLF14_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOT71524.1"
                     /translation="MDKETKQGIIQKFQLHEGDTGSPEVQIAILTERINHLNEHFKSH
                     KNDHHSRRGLLKMVGQRRGLLNYLMKEDIERYRAVVKELGLRK"
     gene            8561..>8871
                     /locus_tag="PLF14_09355"
     CDS             8561..>8871
                     /locus_tag="PLF14_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005356762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOT71525.1"
                     /translation="MHENHKKHVINYAGKEVIIETGKHAQLANGSCLVRYGDTVVLAT
                     ITASKEPRPGIDFFPLSIDYEEKMYSVGKIPGSRNRREGRPSDAAILVSRVIDRPMRP
                     L"
BASE COUNT         2659 a         1751 c         1968 g         2493 t
ORIGIN      
        1 ccttgagtgc aaagttaaat tccagtataa aggagcggca ctggatttta atcttacggg
       61 aagatttatg gacttttctg ttatacaatt atcaaaccgg cagtcacaag agaggaggtc
      121 aaacatgacc gctatttata gaaattctaa taagaataaa cacttaacat tagaggaaag
      181 aataattatt gaaagaagta ttgaaaaagg ttctactaaa acagctatag caaaagtact
      241 gggtaaggat aactcgactg ttggcaaaga aattgctcag catcgtacac taacctatcc
      301 ttgtgcactt ccattagaat gtgcagtgta taaaaaatgt aagcatggta gactgtgtca
      361 caaagattgt ccggactatc gaccattcta ttgcccaaga cgagacagat ctccaggtgc
      421 ttgtaacgga tgtcccacat atgctaattg tcgttttagc aaatacagat atgaagcgta
      481 taaagcacaa gctaattaca aggaagttct tgtaggcaca agaaaagggt tagatttgga
      541 caaagatgat gtcgacagat tagcaagtat cgttgtacca gctattaaaa aaggtttgtc
      601 tccataccat attgttcaca tgtatccgga tatagggatt tctgaaaaaa cgctttacaa
      661 ctacattgct caaggtgttt ttagaccttg gaatgttatt gatttagacc ttagacaaaa
      721 ggtaaaacgc aaacaaccaa aaaaggaaac cgttacatac aagaaaagag aagataaaaa
      781 gcacttaaaa ggacggttat acactgatta tctacaatac atcagtgaag aacctgattt
      841 aagtaccgtc caaatggata ccatctacaa tcgaattgaa ggtccattta tacagacttt
      901 tcatttcttg gaatacgatt tctctttcgg tttttttcat gagaaaagaa ccgcagagaa
      961 tatggtaaaa ggcataaacc ttttggaaga cattatcggc aaagatttgt ttatccaaca
     1021 tatcaggatt ttactaaccg atcgcggagg tgagttctcc aatccggaag ctatggagaa
     1081 agattctgac ggattaactc gaacacgcat cttctattgt gatcctcagg ctgcatggca
     1141 aaaaggagct ttggaaaaac gccacactga gtggcgttac atcctgccta aagaaatagg
     1201 acttagagag ctcgggctct tgggacaaga tgatctcaat ctcgtgctct ctcatctgaa
     1261 ttccgttcca agggagaaga ctcgagggaa gtcacctttt gagcttctcc agttcttctg
     1321 cccggaacta ctgggcgcat ttactgattt tggtatccac gaaataccaa aagacgaagt
     1381 aattttgatg ccgtacttac ttaaaaagta aggcttcata gtgactgccg gcaaacaagt
     1441 tcatagtact ccaacaagtg tcatttagtc ttcataaaaa attgtctgct aaaggtcact
     1501 cgcatttgtg cgctcttttt gcacccttct atgattgagt atatagcctt gtagacaaca
     1561 tttcaagtaa aagacagaaa atacttgcta tcatcatgtg gtaaatctcc atgaatatcg
     1621 gtattttaat taactgtatt actaaaatcc atttttttga aaatctctct tatactggaa
     1681 tttaactttg cactcaagca ggatgaataa ccgctgcatt gttgtaatat ttatgtgtcg
     1741 tttccattaa aaaagcaccc gcatagcaag tgccttttct gattggtgac ccatagggga
     1801 ctcgaacccc tgttaccgcc gtgagagggc gatgtcttaa ccgcttgacc aatgggccga
     1861 gcataaatat gatatgtatt tcctccgtgc ctgtcaagag ttataaaaga aatacggatg
     1921 ataatacttg tttaagttga catccgtaat ctcgtgattc aattatatca ttgtataatt
     1981 atctaatttg cttttactat ataaacaggt ttacatcaga ttcttcggca tcacccaggt
     2041 aggagcctac accggcaagt tccacaccgt caatcagttc ctcgggttta atacccatta
     2101 catccatact catggtacag gctactatct ttacaccctg tttcattgcc ttttcaatga
     2161 gggtttccag cgaatccacg tttttgtctt tcattatctt tttcatcatg gctgttccca
     2221 taccgcccat attcattttg gaaagggaaa gttttttgct tccgcgaggg agcatccgac
     2281 cgaacatagc ttccatgaat gttttcttca ccggttgttt tttggcttta cgaagtgccg
     2341 tcagtcccca gaaagtgaag aacatggtaa caggtctacc cattgctgcc gcaccgttag
     2401 cgatgatgaa gcttgcaagc actttatcca aatcgcccga gaaaacaatt atggttttac
     2461 cgtcggcttc tttagtaaca acggttttgt cgctgtttga acctttcttt accgttgcaa
     2521 cataagtatt tccttttttc tcgtttgtga gcagggtatt gccggtacgt ctgcaccagg
     2581 caccgatatc tttcataaat ccgggatcgg aagcggttac ctccagaatt tcgccgtctt
     2641 tcatatcttg cagtgtttca aagaccttca taatgggacc gggacactgc aaacctgtac
     2701 aatctatact tattgatgcc gttgaagaaa tatatttttt gatatccccg ctgtcggaaa
     2761 caggaagagc atttcttttc acattatcgt atttatcgta atgagtggac ctgtaaaaac
     2821 gcgttcctgc agggtaaagc agcacatcgg taaaaccgtt ctgcattagt attctggttg
     2881 cattgtatga acgaacaccg atggcacaga agactatggt acgtttattc ttatccagtt
     2941 cgtttattct gtcgcgaagc tgacctaacg gaatgtgctt tgctccggga agagcaaatg
     3001 ccattatttc cgcttcttct cggacatcca gcagtaccgc atcgggattg ttttccacag
     3061 cattccactc tgaaaagcgt actataccct tcattatgtt ttccgcaaca aagcccgcca
     3121 tatttaccgg atcttttgcc gaagaatagg gaggtgcgta agcaagctcc agttccttca
     3181 attgcgaaac accgccgccc agccggatag ccgtggcgat tacatcaata cgtttatcca
     3241 ctccgtctgc acctacaatc tgtgcaccga acagtttttt accgtctgcc gagaacagca
     3301 gtttaagtgt cataggtacc gcaccggggt agtaacttgc gtgggaattc tgcattataa
     3361 gtaccgtttc gtagtctttg ccttttttaa gtccctgctt tataagggtt ttctcattgg
     3421 caccggttga agctgcggtt aaatcaaaaa tctttgccac ggaagtgcct tgtgtccctt
     3481 tgtaagtctt acttccgccg gcaatgttgt ccgctgctat acgtccctgt ttatttgccg
     3541 gtccggcaag cggtaccatg gtgcgctctt tggaaatata gtcttccacc tctataacat
     3601 cgcctacagc atagatatca gggtcggatg tgcgcaaata ctcatctgtt acaataccgc
     3661 cgcgtgcatt taatttaaga ccggcttctt ttgccaattt gctgttgggt cgcaccccta
     3721 tggagagtat taccagatcg gcgcttgccg ttttgccgct gcttaatgta attattactt
     3781 tatcgtcttt atctgcaaaa gatgtgacac cgtctgcaag atacagattt acaccgttac
     3841 ccgttatatt ctcatgcaac aactgtgcca tctcatagtc taaaggtgcc ataacctggt
     3901 cctgcatttc cattaccgac acttccagac cggcatgttg caaattctcc gccatttcca
     3961 ggcctatgaa gccgccgcct attaccgccg ctgtttttac gttttgttgc tgtatcattg
     4021 cctttatatc gtccatgtcc ggtaccgtcc acagcgtctt tatgcgtgga gagtcaatgc
     4081 ccggtatggg cggacgtaag ggtgaggagc ctgtggatat taccagagtg tcatagcttt
     4141 cttgatatat gcgtcccgta tcaatctctt tgaccgacac actctttttt ttgcggtcta
     4201 tagcaattac ttcgttctta acccgtacat ccaagcgaaa tctctgtcgc atgttttccg
     4261 gggtctgcag gaaaagcgct tttcttgatt ttatgacatc gcctatatga tagggcagcc
     4321 cgcagtttgc gtaagatata tattcgccgc gctccagaat aacgattttt gcattttcat
     4381 ccaatcttct taaacgggcc gcgcaacttg cacctcccgc aactccgccg ataataacag
     4441 ttttactcat gttcctcctc cttacggttt tttctcttac aggaacgatc ggatattatg
     4501 cgtccgagca accctcctaa cagagcgaag tacagtgtag agataacggg attggatgtt
     4561 ataacgcatg ttccgcttgc acatcctaca aagagatagt acagatatcc cgctccggcg
     4621 cctataattg cagctattat gaccggtaag tatcttttta atatgtctct gaatttgtac
     4681 ttcatgtagt taaacctctt tcttacttaa gtatagagaa aagagagaaa aaactctgtg
     4741 agtatgtcac agaagaaagt tttttttaac atattttcta aagagttttt tatgaaaaca
     4801 cctgtaactt tcgtgttaca ggtgtcatgc gatggtagcg aaggtcagat tcgaaccaac
     4861 gacctaccgg gtatgaaccg gttgctctag ccaactgagc tacttcgccg ttaatattgc
     4921 acgggtaatt ctaacaaaag gcatattgtt tgtcaacagg aattaatata tactcattat
     4981 atatggaggt ttgtatgctt tatcctttaa agtttgagcc cgtatacaaa gactatttat
     5041 ggggcggcaa taatttaact aaattaaaca aaaaacttat ataccctatc acagcggaga
     5101 gttgggaaat atccgccaat aaagacggaa tgagtattgt ttctaacggt aatctggcgg
     5161 gcagcactct tttggacatt tttcaacgct atcccaagaa actgtgcggt aaaagagaag
     5221 taaaacagtt tcctttgctc attaaacttt tagatgcctg ctcggcatta tcggtgcagg
     5281 tacatcccga tgatgcttat gcttatgcca atgaaaacgg tgctctgggg aaaaatgaga
     5341 tgtggtatgt ggtggatgca aaacccgatg caagacttgt atacgggctg aaaaaaggtg
     5401 ttactaaaga gcaattcgca aaagccgttc gtcagaataa tatagggagt acattgaatt
     5461 atgtttccgt aaagaagggc gactggatta atattcctgc cgggttggtt catgcaatag
     5521 gagcgggaat tataattata gaaatacagc agaactccaa tacaacatac agggtatatg
     5581 attttgacag agtgggagcc gacggcaaaa agcggcctct gcatatagag aaagcactgg
     5641 atgtcattaa ttttgacgct accgagatgc agattaatga aatagtgagt gtttcgggtg
     5701 tcgattatct ggcatataat aaatattttg cagtggagta catagcttcg gaagggcaat
     5761 acaagagtga tacattgaat ctgtctttcc acacatatac cgtaattgag ggaggttttg
     5821 aggtggaagg cgtgtcggtc ggattgggag aatcggtact gattcccgca tgcatgaaga
     5881 aatatacctt aaaaggcggc tttaaagcaa taaaaagttt tgttccggac ataaaataga
     5941 tttgtccgtt tattgataca gaagcatatg atattgtctt ttttcctatc gggaaggagg
     6001 catttatgta aaataactat tgaattggat gtttcggtat agtacaataa ctaccgcatt
     6061 gattccttgc cccgataaaa aggggccgat tagaccggaa ggaggtaaac catatgaatc
     6121 agtatgaaac aatttttatt gttgactgta caatggaaga agaagttcaa aaaagcatag
     6181 tggaaaaaat ggttcagcag atgactgatg ccggctgtgt agtggaaaag gttgatgagt
     6241 ggggaagaag gaaacttgct tatccgataa attacaagac ggacggctat tatgtgctgg
     6301 tgacatttaa ggctgcaccg aacttcattt ccgaactgga gcgattgtac aggataacgg
     6361 aaggcatttt aaaaggccag acaatcaggt tataggaggg aaatatgaat aaggtcatat
     6421 taatgggacg acttacaaga gaccctgaat taagaacaac ggcaaccggt acgccggtag
     6481 catcgttttc tttagcagtt aacagaagat ttgccgctaa agatgctcag gtacaggcgg
     6541 atttctttaa cgtaaccgcg tggtccaagc agggcgaatt tgtcaataac tattatcgta
     6601 agggtatgca ggttgtcgta ataggaagac tgcaaaatcg tacttgggaa gatcaggaag
     6661 gtaagaagag atatgctacc gatgtaattc tcgaagaaac gtattttgcg gaaggtaaga
     6721 gagactcggg cgtaagcagc accggaagca atgcgggaga ggatatggat ttcacacata
     6781 tcgatatctc cgaagatgat gctttgccgt tttaatgaga caagaatgaa aggagcggac
     6841 atataatgga tcgcatggat aaagaagata ggaaagattt taaattcaga aggcccaaga
     6901 agaagatttg ccagttctgt gccgataaaa atactataga ttataaagac ttgaatacgg
     6961 tgaaaagata tatttccgaa agaggaaaga ttcttcccag aagagttacg ggcacatgtg
     7021 caaagcatca aagggatgtg actattgcgg ttaagagagc aagacagatt gccttactgc
     7081 cgtatgttac cgataactga gattaaagag agcgcaagct cttttttttc gcccttatgt
     7141 gcaatttcat cacatcggcg gataggacat tacactttgt ctttatggat gatataatgg
     7201 ataaaagcaa taaaaaggag ccataacatg aacattctaa taatgaccga tcttgaaggt
     7261 atcagcggta tctatgcatc cgatcaggta ttaccgggag agagaagatt taacgaagga
     7321 cgtgaattta tgacggcgga tatcaatgcc tgtgcacgcg gacttaaacg cgcaggtgca
     7381 aagaaagtat atgtacgcga ctgtcacggc ggcagctata cattgattca ggagcggata
     7441 tcagaggatg tagactatct gatactgggc gatgtcggac aagatcgctt tttgggactg
     7501 gacgactgtc aggcggtgat attactggga tatcacgcaa tggcgggaac atcgtcggct
     7561 attttagaac acaccatgtc cagcagctat gttcagaact actggataaa cggtaaaaag
     7621 gcgggagagt ttgcgataga tgccggtata gccggggata tgggaaaacc cgtaataatg
     7681 gtgtcgggag atgacaaatt gtgctccgaa gccaagtcgc tggttcctaa tatatactgt
     7741 gccgaagtaa aaaaaggtgt caccgctttc ggcggagtgt tgctgccgct tgaaaaggct
     7801 cgtaagctca tagaagatac ggcatatgag gcatataaaa acatggataa gattaaaccg
     7861 ctggtattta aaaagcccat cagattgcgt gtggagcgtg tggagcgggg cagattgccg
     7921 cttttggcat ccaagccgta tatgaagata atcgatgccc gtacctatga ggtcaaagcg
     7981 gatactatga acgaagcatt gttccgcatc tgaccaaaaa gatattgacc aaaaagatat
     8041 ttgacatgat tgtgttataa tgttaatata tgcgatgaac cttaggctat gtgtatggaa
     8101 actccgacct tacacgttgc ttatggggat aatataaaag gagaaaaata aatggacaag
     8161 gaaacaaaac agggaattat tcagaaattt caattgcacg agggagatac cggatcaccc
     8221 gaagtgcaga tagctattct gacagagaga attaatcatc tgaacgaaca ctttaaatct
     8281 cacaagaacg accaccattc cagaagagga ttgttgaaga tggtcggaca aagaagggga
     8341 ttgctcaact atctgatgaa agaagatatt gagagatatc gtgcggtggt taaagaattg
     8401 ggactcagaa agtaagacaa atacggcgaa ttctcgccgt tttgcatata tccgggggta
     8461 tttttcttaa aaaccgagat taaagtccga aattgcggcg ggttttaata tctgtttttc
     8521 aaggcgaata tctccggaca gaattgtaaa ggagataatt atgcacgaaa atcacaaaaa
     8581 acatgtaatt aactatgcgg gcaaagaagt aataatagag acaggtaagc atgcccagct
     8641 tgccaacgga tcctgccttg taaggtacgg cgatacggtt gtgcttgcca caataaccgc
     8701 atccaaagaa cccagaccgg gtattgattt ctttccgtta agtattgact acgaagagaa
     8761 aatgtattca gtcggaaaaa ttcccggaag cagaaacaga agagaaggca gaccttccga
     8821 tgcggcaatt ttagtttcca gagtaataga cagacctatg agaccgcttt t
//
LOCUS       DAOSOM010000060         8858 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_7867_length_8858_cov_5.026468, whole genome shotgun sequence.
ACCESSION   DAOSOM010000060 DAOSOM010000000
VERSION     DAOSOM010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8858
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7867_length_8858_cov_5.026468"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="PLF14_09360"
     CDS             complement(<1..1088)
                     /locus_tag="PLF14_09360"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOT71526.1"
                     /translation="MKKYYPRIADKILKEELQAFGAVLINGPKWCGKTTTAKQICKSV
                     INMQDPANKANYLKTAKINPLILLQGDKPRLIDEWQEAPELWDAIRYDIDEKQQEGLY
                     ILTGSTKIDDIKTSHSGVGRISTITMKTLSLYESEDSDGSVSLQELFDGKSFEMTKSE
                     LSIRDISEVIVRGGWPNSIGKDLKIASRQIAGYIDSIVKSEINTIDGVERNQAKTRAV
                     LRSLARHTASLATDSKIVADVEANHFTIHRNTLSDYLKVLRDLYVVEDLPAWSPKLRS
                     KTTIRTSNKRHFSDPAFAASLLDAAPEDLLRDIETFGLLFESLVIRDLRIYTDYIGGH
                     VYHYRDKNGLEADAIIHLNDGRWAAVEV"
     gene            1879..3156
                     /locus_tag="PLF14_09365"
     CDS             1879..3156
                     /locus_tag="PLF14_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009142534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOT71527.1"
                     /translation="MTYLKRIADSLLSEKLQAFGAVLIVGPKGCGKTTTAKQQAKSVI
                     EFQDEDNRDKYLSVANEFPSKLLIGEKPRLFDEWQDAPKIWGAIRKSIDDEQLVGAYI
                     LTGSSSRFVETPHTGTGRISTLKMYPMSLYESGESGGEVSLTQLFEDKSMDFVESKLT
                     IDQLIFAICRGGWPRSVNSQNYNSKLAIAEDLVYQTAYVDISNIDKTKRNPLWSQNIL
                     KAYSRNICSLAETKTIFGDVAATTEMTKSTYYSYIEALEKLYIIEDIPAWCPAIRSRT
                     AIRSSKKRNLIDPSIAVAALGLSPEYFNTDFKTLGFLFESLCIRDLKIYSSKMRGEMA
                     HYRDKYGLEADGVLHLQDGRYALLEFKLGAKAIDEGAKHLCKIEKLIGIYNEKEPQCP
                     LRLPDLKIVITATKYGYKRDDGVFVIPIGCLKP"
     gene            complement(3159..4205)
                     /locus_tag="PLF14_09370"
     CDS             complement(3159..4205)
                     /locus_tag="PLF14_09370"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71528.1"
                     /translation="MENRERMVKFVGQHEPICLHLELSLKYLDDDDLEIMKDYGGLKY
                     GNAISRDILIPYEMPLNALHYTIQKLFGWRNSHLRHFEIRKEDFDRLTENNVINWLSL
                     IGTLFKKPAYDDDDIELDEFEFKEGDDEDEWVRDKYTGPYRYRGITDNYDDYKSFADG
                     YREKLSKDRNISHSLTDTLEANIEFMHTWNNLMEGLEVVSVLAPKDQPLHSLSDLGKI
                     MVRRMYQPITGNLWPQMLPVTHKLSYEYDYGDGWTVDITRVRDVYCLSELGLISEDEL
                     QSAMDIVPIIHRPVCLVTNGGPVLDDVGGMYGYCRMLETIFIKRDKRERNFMLDWARS
                     QGWETNNIKEPMVL"
     gene            complement(4816..5301)
                     /locus_tag="PLF14_09375"
     CDS             complement(4816..5301)
                     /locus_tag="PLF14_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71529.1"
                     /translation="MKRKAFMSVLVLALVSMIFILSGCGTITSPSDTGYWFSKSENKY
                     IPFDADKYNLDEAGSYWYFTAAKDVDTTMKVKINVDDHHSALYLYVNNEQVKSETDLG
                     IFSRVYNLSLKKGDELKLHAFWVNGIAAENGSAFEIYMITMSEDGKDYVLTEFDKSTT
                     R"
     gene            complement(5561..6091)
                     /locus_tag="PLF14_09380"
     CDS             complement(5561..6091)
                     /locus_tag="PLF14_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71530.1"
                     /translation="MFVGFESTLKEKSLDDTNNEYLVQNEVVCIDFDKCKNDYCEHSP
                     GENKCGYLKSVDAIIERGSKVYFIEFKNTKIDEKLVYEIVEKCYDSLLLYFEKTGKNI
                     SDSRQNFSFTLVTNYGNKADAVNSKEMIVQTVVGKADEKWKKYKKKFESFFFIGMNIF
                     EPVVFNNYLIKERLIP"
     gene            complement(6093..7412)
                     /locus_tag="PLF14_09385"
     CDS             complement(6093..7412)
                     /locus_tag="PLF14_09385"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT71531.1"
                     /translation="MKIRLKNIGLIDNSEVEINGITIIAGINATGKSTIGKALYSVFN
                     SLQNIYDKVNEIRHKNIMSIFLKDTKDSRLVGHYRNDIFNQYDDQVHYLVENASKFVN
                     DKSALFDNIDSFLDSVSVYPTEVKEIGFNDTMPFTVKESTQNIVDNIFDSLNIPLDRI
                     VKSILQTNLDAEFSGEISNRYISEKVSEITLTIKGEETTVSINDDKVSGIRNIKSMYK
                     DIVYIDDPYIIDDLKYNTDIDILNHFFRRRIYKGRMHHRASLSMKLLANNPDLLESIK
                     TDDKLKDIYAKMEKIKLGNLVYKERAYKYEADNIALDVGNIAAGLKTFIIIKTLLENG
                     SLESNGIMILDEPEIHLHPKWQLVLAELIVLIQKEFNMHILLNTHSPYFLSAIETYSK
                     QHGVSDKCKYYLMNSNEENTVYAEDVTDNTEQIYKLLADPFRTLELL"
     gene            complement(7941..>8858)
                     /locus_tag="PLF14_09390"
     CDS             complement(7941..>8858)
                     /locus_tag="PLF14_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71532.1"
                     /translation="EEGKEFGAKGISRSTNLFDLGLLAESLGIEYDFSDFIINVNGNA
                     HSTATGISIYFPTEQKELLDTYSDICTNKRYIDFLKEYFAYQPETAIAFTNRGYSADG
                     SHLSFTLSKQSISYVQSVGYELHSYAGSEQTGKLYSVGTDNDISLSDGIYTVDFDGRW
                     VYLNDMLLHCDVYQEEDTYTVFSSRVLVNGESASLLFTYSGSTKQIAVDGYILSSDTS
                     SRIYELKPGTKISILYKDALSDDDAPYYEEGAVIWGEDTILKVRNLDIGRYQYIPYVV
                     DIYGNVFYANTATVYFDGTKSVIEDISAG"
BASE COUNT         2776 a         1778 c         1404 g         2900 t
ORIGIN      
        1 ttcacttcaa ccgctgccca tctgccgtcg tttaagtgaa tgattgcatc tgcctccagt
       61 ccgtttttat cgcgataatg ataaacatgc ccacctatat agtcggtata tattctcaag
      121 tctctgataa ctaaagactc aaataacagt ccaaatgttt caatatccct taacaggtct
      181 tcaggagcag catctaaaag tgacgctgcg aatgcgggat cggaaaaatg tcttttgttt
      241 gatgtacgga ttgttgtctt gcttctcagt ttgggactcc atgccggcaa atcctctaca
      301 acatataagt ctctaagtac cttcaagtaa tcgcttaatg tgtttctgtg aatggtaaaa
      361 tgatttgctt caacatcagc aactatttta ctgtcggtag ctaatgatgc ggtatgtctt
      421 gcaagcgatc ttaacactgc ccttgttttt gcttgatttc tttccactcc gtcaattgtg
      481 ttaatttcac tttttacgat actatcaata tatccggcta tctggcggga agctatcttt
      541 aaatctttcc cgatactgtt aggccaaccg cctcttacaa taacttctga aatatccctt
      601 attgataatt cggattttgt catttcaaac gattttccgt caaacaactc ctgcaaactg
      661 acacttccgt cggaatcttc cgactcatac aaagacaaag tcttcatggt aattgttgaa
      721 attcttccca caccggaatg gcttgtttta atgtcatcaa ttttcgtaga cccggtcaat
      781 atgtacaacc cttcttgctg cttctcgtca atatcatatc tgattgcatc ccaaagttcc
      841 ggcgcttcct gccattcatc tatcagcctc ggcttatctc cttgtaacaa tatgagggga
      901 tttattttgg cagttttcaa atagtttgct ttattggcag gatcctgcat gtttattacg
      961 ctcttacaaa tttgttttgc agttgtcgtt ttgccgcacc atttaggtcc gttaataagt
     1021 actgccccaa aagcttgtaa ctcttcttta agtattttat ctgctattct cggataatat
     1081 tttttcattt cattcacctg ttaacatgat accatcactc accggaatta tcaacacaaa
     1141 tgtgcaagtc ggcgcacttt tgatgtgcaa gtcggagcgc ttttgatgtg caagtcggct
     1201 tgtcatagtg tatgttatta gccattgaat atcatatatc tttttcgtac aatagacatc
     1261 ggaggctatt atatggcaca cagtgagcag caatggtaaa aagcaaaaaa actttgccga
     1321 ctgaatgatg aagatattga aagagccaag cgtctgggtc tcaatcccaa aagccttatc
     1381 aaaaacattc cgaacaaatc gcaacaatgg aaagcaccgg tggatgtatg gcttagagac
     1441 attgaggaga aacgaaaaga gaaaaaattg aggaagcaga aatccgcgtt taccgaaaat
     1501 gacgagtagg ccgcatttga ttgcaaacgg agatatttac agttctgtta aataacagga
     1561 atatatattt ctatttttgc acgaaaccgt tacattttcc cctgttatgc gtcataatag
     1621 aaacggtgcg agaatattca atggaattat acaaagaact tgcctcttta gaattatacg
     1681 tttagggagt tatatcaaaa aaccggctat atcctttagg cattgcagga aatgcagaac
     1741 aggcaggcat aaacaaaata ccgcaaatgt gtatgataaa acaccgcaaa tgtgtataat
     1801 aaaatgcctc aaatgtgtat agcaaaactt gacaaatgtg tttaataacg catatgctat
     1861 tcttgaggtg acaattacat gacataccta aaacgaatag ccgattcatt attatcggaa
     1921 aaattgcaag cattcggagc agttttaatc gtaggcccga aaggttgcgg taaaacaaca
     1981 accgctaagc aacaagcaaa aagtgttatt gagtttcagg atgaggataa tagagataaa
     2041 tatctatcag tagcaaatga atttccgtca aagttgttga ttggcgaaaa acccagattg
     2101 tttgacgaat ggcaagatgc tccgaaaatt tggggtgcta tcagaaaatc tattgatgat
     2161 gagcaacttg taggagcata tatattgacc ggctcttctt cccgattcgt tgaaactccc
     2221 cataccggaa caggaagaat ttcaacttta aaaatgtatc ctatgagcct ttacgagagt
     2281 ggtgaatccg gtggagaagt ttctttaact caactgtttg aagataaatc aatggatttt
     2341 gtggaatcaa aattgactat tgaccaattg atttttgcca tatgtcgtgg aggatggcca
     2401 agaagtgtta atagccaaaa ctacaactca aaacttgcta ttgcagaaga tttagtgtat
     2461 caaaccgctt atgttgatat ctccaacata gacaaaacta aacgaaatcc tctttggagt
     2521 cagaatatct tgaaagcata ttccagaaat atttgctctc ttgccgagac taaaacaata
     2581 ttcggcgatg ttgccgcaac aacggaaatg acaaaatcga cttattatag ctacattgaa
     2641 gcattggaaa aattatacat catagaagat attccggcct ggtgcccggc aattcgttcg
     2701 cgtacagcca tacgttcatc taaaaaaagg aatcttatcg acccgtcaat tgcagtagcg
     2761 gccctaggac tgtcaccgga atattttaat accgatttta agactttagg atttttattt
     2821 gaatcgctat gtataagaga tttaaaaatc tattcttcta aaatgagagg cgaaatggca
     2881 cattaccgtg ataaatacgg attggaagct gacggcgttt tacatttgca agatggaaga
     2941 tacgccctgc ttgagttcaa gctcggcgca aaagcaattg atgaaggagc taaacacctt
     3001 tgcaagattg aaaaactcat aggtatttat aacgagaaag aacctcagtg tcctctaaga
     3061 ttaccggatt taaaaatagt aatcaccgca accaaatacg gttacaaacg tgatgacgga
     3121 gtgtttgtta ttcccatagg atgccttaag ccgtaacttt aaagcaccat aggttctttt
     3181 atgttatttg tttcccatcc ttgagaacgt gcccagtcca acatgaagtt tctttctctt
     3241 ttgtctcttt ttataaagat agtttccaac attctgcaat agccgtacat gccgccgaca
     3301 tcatctaaaa ccggtcctcc gttggtcaca agacaaacag gtctgtgaat tatagggact
     3361 atatccattg cactttgtaa ttcatcctct gatatcaatc ccaattccga taaacagtac
     3421 acatctctca cccttgttat atccacagtc caaccgtcac cgtaatcgta ctcatatgaa
     3481 agtttatgtg ttaccggcaa catctgaggc cacaggtttc ccgttatcgg ctgatacatt
     3541 cttcttacca ttatcttgcc taaatcggac agtgaatgaa gcggctgatc tttaggtgca
     3601 agaaccgata ccacttccag accttccata aggttattcc atgtgtgcat aaactcgata
     3661 ttggcttcaa gcgtatctgt cagactgtgg cttatatttc tatctttact taatttttcc
     3721 ctataaccgt cagcaaagct tttataatcg tcataattat ccgtaatacc tctgtatctg
     3781 tacggaccgg tatacttatc cctaacccat tcatcctcat catcgccttc tttaaattcg
     3841 aactcatcca gttcaatatc atcgtcatca taagcgggct ttttaaataa agttcctatc
     3901 agtgacaacc aattaataac attattctct gttaagcgat caaaatcttc tttccgtatt
     3961 tcaaaatgtc tgagatgact gtttctccaa ccgaaaagtt tttgtatcgt ataatgtaaa
     4021 gcattcaaag gcatctcata cggtattaga atatctctgg atatggcatt accgtattta
     4081 agaccgccgt aatctttcat aatctccagg tcatcatcat caagatattt caacgacaac
     4141 tctaaatgca gacaaatagg ttcatgctga ccgacaaatt tgaccattct ttctctgttc
     4201 tccatgacat acctctctct ttttttattt atatgctaca aacagataat accatatagc
     4261 ggttaaaaaa aacacctact ttacacattt gcggtatttt gccgaacaca tttgcgccat
     4321 tttgccgaaa acatttgagg caacacagga tgtttgttgt tgcagtcccg tgttagcata
     4381 taaatagtac aactggaaca aacggatttc tcaagtataa tatatctaag tgagaaagca
     4441 gttatgtcaa aacatcgtat gaccgaagat gttatctcat tgtttaattc gacttcaaat
     4501 tcgttaaaca gtcacgcttt ttgcaagaat gatagatata tcattttaga caaaaatgat
     4561 tttattgaag atattcagtt ggaatctttt gttaagtctt ctgttaaagt aaaaataaaa
     4621 atcaataaag tagaaaaaat ccctatcact ctatgagaaa gtttaaaaag gggacattat
     4681 tgaaaactgt ccggtagtta taataccgcc tataggcgat attgatgatg gagatgaacc
     4741 ggaagcagaa ataaaaaagg aagcccgttt ccgaacctcc tttataccgg ttattaatat
     4801 ttcttaaaat ataaattatc ttgttgtact cttatcaaat tcagtcaata cgtaatcttt
     4861 tccgtcttcc gacatggtaa tcatgtatat ctcaaaagcg cttccgtttt cagcagctat
     4921 accgtttacc cagaatgcat gcaatttgag ttcatctcct tttttcaatg acaaattata
     4981 tactcttgaa aaaataccta aatcggtttc gcttttaact tgctcattgt tcacatagag
     5041 atatagagcg gaatggtggt catcaacatt tattttcact ttcatagtgg tatctacatc
     5101 ttttgcagct gtaaaatacc agtaactgcc cgcttcatcg agattgtact tatccgcatc
     5161 aaacggtata tacttgtttt ccgacttgga aaaccagtac ccggtatccg acggagatgt
     5221 aattgtaccg caaccggata atataaatat cattgacact aaagccaaca ccagaaccga
     5281 cataaaagct tttcttttca taattcagac tccttttaaa tatattcagc attactacag
     5341 gattaaggct tatgtttttc aacttttatg tattactata ttaactaaac cggttaatat
     5401 acaatacaaa aaaacaataa acctttcctg ttgtcttgca agataattct aactcttgac
     5461 gggattgttt gcaacagtgc aaatattaaa aaagttttta atactcagtt atttatttat
     5521 gagcggaaca aacttggcgg tactgcttaa tattaaatac ttacggaatc aatctctcct
     5581 taatcagata attattaaaa actacaggtt caaatatgtt cattcctata aagaaaaacg
     5641 attcaaactt ttttttatac tttttccatt tttcatcagc tttccctacc actgtctgta
     5701 caatcatttc tttgctgtta acggcatcgg ctttatttcc ataattggta accaatgtaa
     5761 agctaaagtt ttgccttgag tctgaaatat tttttcctgt tttctcgaaa tatagcagca
     5821 atgaatcata acacttttcc actatttcat aaaccagttt ttcatcaatt tttgtgttct
     5881 tgaattctat aaagtatact ttacttcccc tttctataat tgcatcaaca gattttaaat
     5941 aaccgcattt attttcaccc ggagaatgct cacaataatc atttttgcat ttatcaaaat
     6001 ctatacatac aacctcattt tgcacaagat actcattatt tgtgtcatca agagactttt
     6061 cttttaaagt tgattcaaat cctacaaaca ttctataaca actccagcgt tctaaaagga
     6121 tctgctaaca atttatatat ttgctcagta ttatcagtaa catcctcagc atatacggta
     6181 ttttcttcat ttgaattcat caagtaatat ttacacttat cggacactcc atgttgtttg
     6241 gaataagtct ctattgcact gagaaaatag ggactgtgag tattaagaag aatatgcata
     6301 ttaaactctt tttgaattaa aacaattaat tccgctaaaa ccaactgcca tttaggatgc
     6361 aaatgaattt caggttcatc taaaatcatt ataccgttgg actccagact gccgttctca
     6421 agtagtgttt ttataataat aaaagttttt aaccccgctg caatatttcc tacatccaaa
     6481 gcaatattgt cagcctcata cttgtaagca cgttctttgt ataccaaatt cccaagcttg
     6541 attttttcca ttttagcata tatatcttta agtttgtcat cagtttttat actttccaac
     6601 aagtcgggat tatttgccaa caacttcatg ctcaatgaag cacggtgatg catccttcct
     6661 ttatatatcc gcctacggaa aaaatgattt aaaatatcta tatcagtatt gtacttcaga
     6721 tcatcaatta tgtacggatc atcaatatac acaatatctt tgtacataga ttttatattt
     6781 ctaatgcctg aaaccttatc atcattaata ctgactgtcg tttcttcacc cttaatggtc
     6841 agcgtaattt cagacacctt ttcagatata tatctgttac ttatttcccc gctgaattcc
     6901 gcatccaaat ttgtttgcaa aatacttttt acaatcctgt caagcggaat atttaaggaa
     6961 tcgaaaatat tatctacaat attttgagta ctttctttta ctgtaaaagg catagtatcg
     7021 ttgaacccta tttctttcac ttcagtagga taaacgctga ccgaatcgag aaaactgtca
     7081 atattatcaa aaagagcgct tttatcatta acaaacttac ttgcattttc cacaagataa
     7141 tgcacttgat catcatattg attaaatata tcgttacgat aatggcctac aagtcgagaa
     7201 tcttttgtgt ctttcagaaa gatactcata atgttcttgt gtcttatctc gtttaccttg
     7261 tcataaatat tctgaagact attaaagaca ctatataaag ctttgcctat cgtacttttt
     7321 ccggtagcat ttatacctgc aataattgta attccattaa tttcgacttc actattatca
     7381 atcaagccta tatttttcaa tcttattttc acaacttatg cctccgatat ggtgttgcca
     7441 tagattaaac cgacaattaa ctacagtgtc aaaagtaacc tatttataac ttttagttgt
     7501 acatttcaca aagtcatcag tactttacaa cttaacaatg tcttaagttc tttaaccggg
     7561 attaagatta taaaattgtt atgaatataa tctgaatcca tgtaatcata ttgctatcat
     7621 acaattttct ttatatttcc gtcaatagaa atagtgaaag gtattctgct tttcttatca
     7681 ttttgtcata tgttaaatat tatgaaatat atttaaaatg acatagccga aactgtactt
     7741 tcctaaaata ccatattaat tatcttatct tctgaactga ggtatgggaa aatcctcact
     7801 caagtaaccc gcaatctcta atgcttttta cactcccaaa cgataatcgg aaccggctga
     7861 ttaataaatg aacataaacc gctatgcatc tttgcctgca ctgtctcaac tgtacagata
     7921 gtgtaagcat atttaatccg ttatcccgcg gaaatatcct caataacact ttttgttccg
     7981 tcaaagtaca ccgttgcggt atttgcataa aatacgttac cgtatatatc tacaacataa
     8041 ggaatatact gataccggcc tatatcaaga tttcttacct tcagtattgt atcctcaccc
     8101 cagataaccg ccccttcctc ataatacgga gcatcatcat cgctaagcgc atctttatac
     8161 aaaatgctta tcttggttcc cggtttcaat tcgtaaatac gcgaggatgt atcggaggaa
     8221 agtatatacc cgtcaaccgc tatttgctta gttgaaccgg agtaggtaaa taacaatgaa
     8281 gctgattcac cgtttaccaa aacacgggaa gaaaaaacag tatatgtgtc ttcttcctgg
     8341 tacacatcac aatgaagcag catatcgttt aaatagaccc atctgccgtc gaaatctacg
     8401 gtatatatac cgtcggataa ggaaatatca ttatcggtac cgacggaata taatttcccc
     8461 gtttgttcgc ttcccgcata actgtgtaat tcataaccta cagactgaac atagcttatc
     8521 gattgtttag acagagtaaa agaaagatgt gatccgtccg cactgtaacc tctgtttgta
     8581 aaggcaattg ctgtttcagg ttgataagca aaatactctt taagaaagtc tatgtacctt
     8641 ttattggtgc aaatatcgga atatgtatcc agcaactctt tctgctctgt tggaaagtat
     8701 atacttatac ctgttgcagt actatgtgca ttaccgttta cattgataat aaaatcggaa
     8761 aagtcatact ctatacctaa agattcggca agaagaccga gatcaaacaa attggtactg
     8821 cgacttattc cctttgcacc gaattctttt ccctcttc
//
LOCUS       DAOSOM010000061         7915 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_9140_length_7915_cov_6.649618, whole genome shotgun sequence.
ACCESSION   DAOSOM010000061 DAOSOM010000000
VERSION     DAOSOM010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7915
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9140_length_7915_cov_6.649618"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            286..702
                     /locus_tag="PLF14_09395"
     CDS             286..702
                     /locus_tag="PLF14_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009644963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOT71533.1"
                     /translation="MKLIYPAIFTACEEKEGYTVEVPDLPGCVTEGDSLSDAIEMGTD
                     AAGGWILTELEEGRSYPKASEQTDIIIPEGSFAGLLALDIDAYAEKYGNKAVRKNITI
                     PAWLNTYGEKNNINFSKLLTDALMERALSEQKRTVK"
     gene            complement(884..1471)
                     /locus_tag="PLF14_09400"
     CDS             complement(884..1471)
                     /locus_tag="PLF14_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009537578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71534.1"
                     /translation="MVVTTSMLIEQYGNYRNPKSKIRRLVDNGELFPLTRGIYETNKD
                     AKGHYLAGVIYGPSYLSFDYALSVYDLIPEAVYEYTSATFEKKKRKLYENLFGRFSYR
                     DVPADVFPFGILIREENGYAYRIATAEKALCDKLYIMPPVTNQRDIEEMLFEDLRIDE
                     YEFAKLDRNFILKTGEKYHCNNLKYLMKYMRRRTF"
     gene            complement(1633..1866)
                     /locus_tag="PLF14_09405"
     CDS             complement(1633..1866)
                     /locus_tag="PLF14_09405"
                     /inference="COORDINATES: protein motif:HMM:NF036995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
                     /protein_id="HOT71535.1"
                     /translation="MHEEKLQQLVGEISFPISFSYIPWGHHILIITKCKTIEEALFYV
                     RRTIDEDLSRKALDDYIRADLYHVSGGALTALD"
     gene            2018..3700
                     /locus_tag="PLF14_09410"
     CDS             2018..3700
                     /locus_tag="PLF14_09410"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013433216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOT71536.1"
                     /translation="MYYSRMLIPTIRETPQEAEAVSHKLMLKAGLIRRLASGVYIFLP
                     LGYKALRNIERIIREEMDKKGALELLMSALIPKELLEQSGRWEVFGPEMFRLKDRSER
                     ELCLGPAHEESFTITARNEIRSVKNLPLTLYQIQTKFRDELRPGHGIIRSREFLMKDA
                     YSFDKDESGLDKSYNDMHDAYCNIFARCGLDVVIAYGAGSEEFMVLSDIGEDKVVTCT
                     VCDYAANQEIAEAVSHNTASSDTLLNMELLHTPNIKTIDDLCSFMGCSSVNFAKTLIL
                     VADGEPVMAVIRGDKELSLKKLAGLLKCDKLSLAENHLVEKLTGAAVGFASPVGMKCK
                     VVLDNQLRNMTNFVTGANKTDYHYINVNLKDFTSYSFGDIRAVCEKDVCPKCGSGIKI
                     SSAVEVGHIFKSGDKYSKALDCVYTDEFGEKKPMIMGCYGIGLDRILAAVIEQHNDEK
                     GIVWPMEVAPHKVYILPTLVSDPVIFDMAVKMHDELVAMGIDVILDDRNERAGVKFND
                     ADLLGIPLRVTVGRNAKDGMVELKKRNEEQAALVTYSRALQSIIENVREYTA"
     gene            complement(3642..4289)
                     /locus_tag="PLF14_09415"
     CDS             complement(3642..4289)
                     /locus_tag="PLF14_09415"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOT71537.1"
                     /translation="MERFEERMEKTADRLKKLYEEYIEKVKQSNKDYDFQFVFSDGLM
                     RKGIMLIGEAPGKDEVAQGKPFVGMAGAKLKEFMESLKLERDDILVTNAVKYRPYKIN
                     PKSGKKSNRTPTKKEIQLSLYCLEKEIEIIKPKLILTLGNVPLRALKKDFTLNIGQWH
                     GKQTEYKGITHFPLYHPASVIYNKSLQSVFEQDLEAVKQYILLHFLLYFVMPGYM"
     gene            complement(4244..4795)
                     /locus_tag="PLF14_09420"
     CDS             complement(4244..4795)
                     /locus_tag="PLF14_09420"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOT71538.1"
                     /translation="MSEWLQLEKQCNECKKCSLWENRKNVVIGRGNKTAAFMLIGEAP
                     GEEEDIQGRAFVGRAGQLLSMALSAMGIEEDNYYICNIIKCRPKNNRTPYEQEIQECL
                     PYLDRQIELVQPEVIVLLGNTAAASLIDKNIRITQQRGTWHEYKGIPVMLTYHPAALL
                     RDESKKIDMWRDLKNAWKRLQTA"
     gene            complement(4802..5749)
                     /gene="whiA"
                     /locus_tag="PLF14_09425"
     CDS             complement(4802..5749)
                     /gene="whiA"
                     /locus_tag="PLF14_09425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HOT71539.1"
                     /translation="MSYTALVKKELSYKNLSKSCCEKALLSAMIRLEGKFTKERNGYF
                     SLRINTEDNSEARWIIFSINRIYSLHSDIRRKKNRFNDKTIFTVKTEASYASENAFSD
                     LGFMDADAKGNGIFTDITEYLLSDCDRYSYYRGAFLACGLIREPKKGYFLEIASKNAT
                     ETDRLYNMLLKESLLNVKRSKRRNTEYIYFCNSEDISTFLNMIGAHNALMDYENARIE
                     KSIMNDTNRMINCDEYNTDKSVETKIRQINAIRYIQGTKGLDFLEEKLKETALLRLNE
                     ELTEYNEIAERLKVKVSKSTVQKRLAKLEGIAKDLGYKG"
     gene            complement(5749..6633)
                     /gene="rapZ"
                     /locus_tag="PLF14_09430"
     CDS             complement(5749..6633)
                     /gene="rapZ"
                     /locus_tag="PLF14_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOT71540.1"
                     /translation="MQLFIITGMSGAGKSYALKTMEDLGFYCMDNIPPSFIPKFVTMV
                     RNNKKIRKVALVTDIRGAEPLENILITLDKLDEQGCKYKLIYLVASDVMLLKRYRESR
                     RHHPMEDETTSITEAIEKEREQFHDISRRADHIIDTSQLLAKEFKRELAEIVLGGPLQ
                     ESLIITLASFGVKYGNPVDCDLIFDLRFTPNPYYIEDMREMTGKDQKVIDYVLGQKAT
                     QDFLKMLTEMVEYLIPLYIKEGKTSLVIGLGCTGGRHRSVVIGDEFGRILEKNNHRVT
                     VQHRDIEKDAIVKEYGKR"
     gene            complement(6633..7520)
                     /gene="murB"
                     /locus_tag="PLF14_09435"
     CDS             complement(6633..7520)
                     /gene="murB"
                     /locus_tag="PLF14_09435"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005212180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOT71541.1"
                     /translation="MKTILDKYDFVKFNEPMSNYTSYKTGGPADIVALPENAESLKAI
                     LTILREYNMPVYLFGNCTNILVRDKGIRGAVILTGRLNDIEVEGNFITASAGVSMAKL
                     AAAAYEASLTGAEAVSGIPGSVGGAVSMNAGSYGTSIEDILVSSQYLDENLNICTLQK
                     EQHQFGYRTSFFTDKNYVILNSTFAFTHGDKSSIKRDMLEYARKRKSSQPLEYPSCGS
                     VFKRPAGHFAGKLIQDAGLKGYTVNGAQVSEKHAGFIINKGGATSKDILTIIDYIKSV
                     VYNKYKVNLELEVKIIGEE"
     gene            complement(7504..>7915)
                     /locus_tag="PLF14_09440"
     CDS             complement(7504..>7915)
                     /locus_tag="PLF14_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HOT71542.1"
                     /translation="RLIADDVVEIRKVSQTTLLGTSPEIIRHFMELRGVGIIDVKGLY
                     GAGSVKDIDSIDLVINIERWDENKKYDRIGMEEKYTEYLGIQIPSLTLPIMPGRNMPI
                     LMEVAAMDFRQKKMGYNAAKEMYERVNRKFENNT"
BASE COUNT         2107 a         1743 c         1589 g         2476 t
ORIGIN      
        1 tttggacagc tcctaaatat tagcatagca atattgcact gtcaacaggt ttttttaact
       61 acctacttgc gcatcggtac cagtaggtta atcagccgta ttacttaagt attcctgccg
      121 atacacaaat gccgtttgac ttatttcaat acatacgcat aatacgcatt ttctgttgac
      181 atatatgcgc ataatgcgta tggtaagatt acggacggat gaaagatgac tgctgcacag
      241 gaaagactca gataatcaat attgaaacaa gcgaggtacc gcaaaatgaa acttatatat
      301 cccgcaatct ttaccgcatg tgaagaaaaa gaaggctata cagtggaagt tcccgatctt
      361 ccgggatgtg tcactgaagg agacagcctc agcgatgcaa tcgagatggg cacagatgcg
      421 gccggcggtt ggattcttac cgaacttgaa gaaggtcgca gctatccgaa ggcaagcgaa
      481 caaaccgata taattatacc cgaaggctca tttgccggtc ttctggcact ggatattgat
      541 gcttacgctg aaaaatacgg aaataaggcg gtgaggaaga atataacaat acctgcatgg
      601 ctcaatacat acggcgagaa aaacaacatt aatttttcaa aactgcttac cgatgcactt
      661 atggaaagag cattgagtga acaaaaaaga acggttaaat agctgaagaa agttctctca
      721 cagtaattct tatatacttt tccggaatca acaggaaaca taaccgaaaa aaaagagaga
      781 cctcgcaagt tctctcccgg ttccaggccc cgcaagcatc tgcaacctaa tcactggaca
      841 taatatatcg taatcgctca tgcttgtcaa catatccttt cgttcaaaaa gttcttctcc
      901 gcatatactt cattaagtac ttcagattat tgcagtgata cttttctcct gtttttaata
      961 tgaagtttct gtccaactta gcaaattcgt actcatcaat gcgtaaatcc tcaaaaagca
     1021 tttcctctat atctctttga ttggtcacgg gcggcattat gtaaagctta tcgcacagtg
     1081 ccttttccgc tgttgcaatc ctgtacgcat atccgttttc ctctcggata agaataccga
     1141 acggaaaaac atccgcagga acatcccgat aagaaaacct tccgaacaga ttctcatata
     1201 gttttctctt cttcttttca aaggttgcag atgtatattc ataaacagct tccgggatta
     1261 aatcgtatac cgaaagagca taatcaaatg acagataaga aggtccgtat ataactcctg
     1321 caagataatg ccccttagca tccttatttg tttcatatat tccccgtgtt agcgggaata
     1381 attcaccatt atctacaaga cgtcgaatct tactcttcgg atttctgtaa ttaccgtatt
     1441 gctcaataag cattgaagtt gttactacca ctatttgctc ctccaagtgc aattatactg
     1501 cacttagcgg agcaaatcaa tagcttcttc caatactatt tcatcatata ctgtaggaag
     1561 gttgataaat ccaaggtcat aattttcctt caaaagctct tgggcaagtt taccctgtgg
     1621 cattggcagg tactaatcaa gagcagtgag cgctccgccg gatacatgat acaaatcggc
     1681 acgaatataa tcgtccaaag ctttacgact caaatcttca tcaatcgtac gtctgacgta
     1741 gaaaagtgcc tcctcaattg ttttgcactt tgtgattatt aatatatgat gtccccatgg
     1801 tatatagctg aaagaaatcg gaaaggatat ttctccgaca agctgttgga gtttttcttc
     1861 atgcatctga gttccaatct gtttactctt gaatttccga accaacctga ttcagtttta
     1921 gacttcccat atcaagacac ttctgcatat ctgaaataag cggaatagtt tccgcttatc
     1981 tccgctatgg tataatatac ggtacacgga gaatgctatg tactattcaa gaatgcttat
     2041 acctacaata agagagactc ctcaggaagc cgaagcggtt tcccataaac tcatgcttaa
     2101 agcagggttg atcaggcgct tggcttcagg tgtatatata ttcttgcctt tagggtacaa
     2161 ggctttgagg aacatagaga ggataatccg ggaggaaatg gataaaaagg gtgcactgga
     2221 acttcttatg tccgccctta tacccaagga acttttggaa cagtcgggaa gatgggaagt
     2281 cttcgggccg gaaatgttcc ggttgaaaga ccgttcggaa agagagctct gtttaggccc
     2341 cgctcatgaa gaatccttta caataaccgc aaggaacgaa atccggtcgg taaagaatct
     2401 gcctttaacc ttgtatcaga tacagaccaa attccgtgac gagttgcgtc ccgggcacgg
     2461 aataatacgt tcaagagagt ttctgatgaa ggatgcatac agtttcgata aggatgagtc
     2521 cgggctggat aagtcatata acgatatgca cgacgcatat tgtaatatat ttgcccgttg
     2581 cggtctggat gtggttattg cctatggggc gggctcggag gaatttatgg tattgagtga
     2641 tataggagaa gataaggtgg taacctgtac ggtatgtgac tacgccgcta atcaggaaat
     2701 agcagaagcg gtaagccata atacagcttc ttccgataca ttgctgaaca tggaactttt
     2761 acacactccg aatataaaga caattgacga cctttgctcc ttcatgggtt gttcttccgt
     2821 taactttgca aaaactttga tactggtagc cgacggcgaa ccggttatgg ctgttataag
     2881 gggggataaa gaactgtcgt tgaaaaagct tgcaggtttg cttaaatgtg acaagctttc
     2941 gcttgcggaa aatcatttgg tggagaaact taccggtgct gcggtgggat ttgcctctcc
     3001 cgtaggcatg aagtgtaaag ttgttttgga taaccagctt aggaatatga ccaacttcgt
     3061 taccggagcc aacaagaccg attaccatta tataaatgta aatcttaagg atttcacaag
     3121 ttattctttc ggggatataa gggcggtatg cgagaaagat gtatgcccca aatgcggtag
     3181 cggtataaag ataagcagtg ccgtggaagt gggacacata ttcaaatcgg gtgacaaata
     3241 ctccaaggct ttggattgtg tgtataccga tgaattcgga gagaaaaagc ccatgattat
     3301 gggctgttac gggataggat tagacagaat actggctgcg gttatagagc agcataacga
     3361 tgaaaaaggc atagtgtggc ccatggaagt tgcaccgcat aaagtatata tattacccac
     3421 attggtaagc gatcccgtca tatttgatat ggcggtcaag atgcatgacg agctggtcgc
     3481 tatgggtatc gatgttatat tagatgaccg caacgagcgg gcgggcgtta agttcaatga
     3541 tgccgatttg ttgggcatac cgttaagagt aactgtgggc agaaatgcca aagacggtat
     3601 ggtggaactt aaaaaacgta atgaagagca ggctgcactt gttacatata gccgggcatt
     3661 acaaagtata atagaaaatg taagagaata tactgcttaa cggcttcaag gtcctgttca
     3721 aatacgcttt gcagactttt gttgtatata acgcttgcgg gatgatacag aggaaaatgt
     3781 gttatgcctt tatattccgt ctgtttaccg tgccactgcc ctatattcaa ggtaaaatct
     3841 tttttcagtg cacgtaaagg aacattaccc aaggtaagta taagtttcgg tttgattatc
     3901 tctatttctt tttccaaaca atacagggaa agttggattt ccttttttgt cggggtacgg
     3961 ttgctctttt tacctgactt gggattaatt ttatacggtc ggtattttac cgcattggtt
     4021 accaatatgt cgtctctttc cagtttcagg gattccatga actctttaag ttttgctccc
     4081 gccattccga caaaaggttt accttgtgca acttcatctt ttcccggagc ttcccctata
     4141 agcataatgc ctttacgcat caagccgtcg gaaaagacaa actggaaatc atagtctttg
     4201 ttgctttgct ttaccttttc tatatattcc tcataaagct tttttaagcg gtctgcagtc
     4261 ttttccatgc gttcttcaaa tctctccaca tatctatctt cttggactca tcccgcaaaa
     4321 gtgccgccgg atgataggta agcataaccg gtatcccttt atactcatgc catgtgcctc
     4381 tttgctgcgt tatccttata tttttatcta taaggcttgc tgctgcggta ttgcccaaaa
     4441 gcactattac ttccggttgc accagttcaa tctgtcggtc cagataaggt aaacactcct
     4501 gtatctcctg ttcatagggg gtgcggttgt ttttaggtct gcattttata atattacaga
     4561 tatagtagtt gtcttcttct atacccattg ccgacaaagc catggataac aactgtcccg
     4621 ctcttcccac gaaagccctg ccctgtatat cctcttcttc cccgggagct tctcctataa
     4681 gcataaaggc ggcagtttta ttgcctctgc ctatgacaac attcttcctg ttttcccata
     4741 aagaacattt cttacattca ttgcactgtt tttccagctg taaccattca ctcatacttc
     4801 gttatccttt atatcccaag tcttttgcaa taccctctaa tttcgccaac cgcttctgca
     4861 cagtggattt tgacaccttt accttaagtc gctcggcaat ctcgttatac tccgtcaact
     4921 cttcgttaag tctcaacagt gccgtttctt taagtttttc ttccagaaaa tccagccctt
     4981 tggtgccttg tatatatctt atggcgttaa tctgtcttat cttggtttcc accgatttgt
     5041 cggtattgta ttcgtcacag tttatcattc tgttggtatc gttcattata cttttttcta
     5101 ttctggcatt ctcataatcc attagggcgt tatgcgctcc tatcatgtta aggaaggtgg
     5161 aaatgtcttc ggaattgcaa aagtaaatat attcggtatt gcgccgctta gaccgcttta
     5221 cgttgagcaa cgattctttc agaagcatgt tatacagcct gtcggtctcg gtcgcattct
     5281 ttgatgctat ttccagaaag tatcctttct tgggttcccg tataagtccg caggcaagaa
     5341 aagcgcctct gtaataggag tatctgtcac aatcggacag cagatactcg gttatatcgg
     5401 taaatatacc gttacctttg gcatccgcat ccatgaaacc cagatcggaa aaagcatttt
     5461 ccgaagcata actggcttcg gtctttacgg taaatatcgt tttatcgtta aatctgttct
     5521 tttttctgcg gatatccgaa tgtaaagaat atattctgtt gatggaaaat ataatccagc
     5581 gggcctcgga attgtcctcg gtgtttatgc gtaaggaaaa gtagccgttt cgttctttgg
     5641 taaactttcc ttccaagcga atcatggcac ttaacagtgc cttctcgcaa cagcttttgg
     5701 ataggttttt atatgacagt tcttttttta ctaaagcggt gtatgacatt atctcttgcc
     5761 atactcttta actatggcat ccttttctat atctctgtgt tgaacggtaa ccctgtgatt
     5821 gtttttttcc aatattctgc cgaactcgtc acctattacc accgaacggt gtcgccctcc
     5881 ggtgcatccc aagcctatga ccagtgaggt ctttccttct ttgatgtaca gaggaatgag
     5941 atactccacc atctcggtca gcatcttaag aaaatcctgt gtggctttct gacccagaac
     6001 atagtctatt accttttggt ctttccccgt catctctctc atgtcttcta tgtaataagg
     6061 gttgggtgta aaacgcaagt cgaatatgag gtcgcagtcc accggattgc cgtattttac
     6121 tccgaaggat gcaagggtga ttatgaggga ttcctgcagc ggtcctccca agacaatctc
     6181 cgccaactcc cgtttaaact cttttgctaa aagctggctg gtatctatta tgtggtcggc
     6241 tcttctgctt atatcatgaa actgttcccg ctctttttct atggcctcgg ttatgcttgt
     6301 ggtctcgtct tccatgggat gatgccttct ggactcgcga tatctcttca aaagcattac
     6361 atcggaagcc acaagatata tcagtttata cttacaaccc tgctcatcca acttatccag
     6421 tgtaatcaat atgttttcca gcggctccgc tccccttata tcggtaacca gcgctacttt
     6481 tcttatcttt ttgttgttgc gtaccattgt aacgaactta ggaataaagg aaggcggtat
     6541 attgtccatg caatagaaac ccaagtcttc catggtctta agtgcgtacg atttgcccgc
     6601 tccgctcatt ccggttatga taaacagttg cattattctt ctccgataat cttaacttcc
     6661 aattcaagat ttactttata tttattatac acaacacttt ttatatagtc tataattgta
     6721 agaatatctt ttgaggttgc acctccttta ttgatgataa aaccggcatg tttttccgac
     6781 acttgcgccc cgtttacggt atagcctttc aatccggcat cctgtataag tttgccggcg
     6841 aaatgtccgg ccggtcgctt gaatacgctt ccgcaggaag gatattccaa aggctgagag
     6901 gattttcttt ttcttgcata ctccagcata tcccgcttta tgctgctttt atcaccatgt
     6961 gtaaacgcaa aagttgaatt gagtattaca taattcttat cggtaaagaa gcttgttctg
     7021 tatccgaatt gatgctgttc tttttgtaaa gtacatatat ttaagttttc atccagatat
     7081 tgcgaactta ccagtatatc ctctattgat gtgccgtacg atccggcatt catggatacc
     7141 gctccgccta ccgagccggg tattcccgac accgcttcgg ctccggttaa ggatgcttca
     7201 tatgccgctg cggccagttt tgccatggaa acacctgcag aggcggttat gaaattgccc
     7261 tcaacttcta tgtcattcaa ccttcccgtt aatattacag ccccccttat gcctttatca
     7321 cgtaccagta tgttggtgca gttgccgaac aggtaaacag gcatattata ttctctcaat
     7381 attgtaagta tcgctttgag agattctgca ttttccggca acgccacaat atctgcaggc
     7441 cctcctgttt tatatgaggt atagttgctc atgggctcat tgaattttac aaaatcatac
     7501 ttgtcaagta ttgttttcaa atttcctgtt caccctttca tacatttctt tggctgcatt
     7561 gtatcccatc ttcttttgcc taaaatccat tgccgccact tccataagta tgggcatgtt
     7621 tcttcccggc attatgggca atgtcaatga aggtatctgt attcccagat actcggtata
     7681 cttttcctcc atacctatgc ggtcgtattt tttattctca tcccatctct ctatgtttat
     7741 aaccagatct atactgtcta tatcttttac cgaacccgct ccgtaaagtc ctttgacatc
     7801 tataataccc actccgcgca attccatgaa atggcgtatt atctcgggcg atgtacccaa
     7861 aagggtggtc tggcttacct tacgtatttc cactacatcg tcggctatga ggcgg
//
LOCUS       DAOSOM010000062         7892 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_9173_length_7892_cov_5.409085, whole genome shotgun sequence.
ACCESSION   DAOSOM010000062 DAOSOM010000000
VERSION     DAOSOM010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7892
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9173_length_7892_cov_5.409085"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..1140
                     /locus_tag="PLF14_09445"
     CDS             85..1140
                     /locus_tag="PLF14_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71543.1"
                     /translation="MLSNEAKKRYRAFWDRTAYERCVLFISERIGKGVVMQGASPEDK
                     WTDLERRTRDAQESYVNFKYYAEGFSTVFTNFGPGSMAACIGGDFELAPSTIWFDRNP
                     IIKDWSEVPELKLYEDSLMWQLMTGLTEMLCKASKCTYFTSMADIGGSMDILSSLRGA
                     EQLLCDLIDYPEEVKKVNERIEELWKIVYSKLYAITSRYQNGMTSWMPIYCEKRYYPL
                     QCDFCAMISPDMFLEFVKPNLSAQAEWLDHSIYHLDGMDAVRHLDHLLDIPAIDAIQW
                     SAGAGKPPLYDECFFDMYNKISRSGKGLVLFSVPLTELENVLKNISTKGLFITMQVSD
                     EKEALEAIKIAKSYGVK"
     gene            1187..2005
                     /locus_tag="PLF14_09450"
     CDS             1187..2005
                     /locus_tag="PLF14_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019004101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOT71544.1"
                     /translation="MSFKIGVITDSFRCPLEQAIDKAAALGADGVQIYATGRELAHDT
                     PDDRVKKILRLLKDAGLELSALCGDIGAFDDEIVNIERVKHSKKIVDLAVKMGTKVVT
                     THIGVVPEDKNHKQYRTIHKACSELGRYADSQGVKFAIETGPEKAYILRMLLDDLDCT
                     GIAVNFDPANLTMCVDDDAREAVRILAPFIVHTHAKDGIMLSKYANDPRYGELAKKRA
                     WLELPLGEGDVGFPTYLEALRKIGYKGFLTIEREVGEDPAKDIANAVTFLRKLI"
     gene            2015..2884
                     /locus_tag="PLF14_09455"
     CDS             2015..2884
                     /locus_tag="PLF14_09455"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOT71545.1"
                     /translation="MKIGISSYSYYKLTRKGLIDCFGIIEKTKEFGFDAIEFSGMPGD
                     TYEERYKMAKDIKKACDDCNLLIANYCTGADLVKGSNWNLQTEIDRIKKEIDIAAVLG
                     VSKMRHDASFGFGATDPKARPLDMYLYRLAEGCKKLTEYAKGANIRTMIENHGRYCQH
                     SCDVLSIYRAVDDDNFGILIDIGNFMCVDQDPVEAVAQLAPYAFHVHAKDFHFKSGIN
                     MNPGWGWFLTKHGNYLRGSIIGHGDVPLKECINILKIYGYDDVLSIEFEGIEDVLSAI
                     ENGQANLKKLVDM"
     gene            complement(2917..3126)
                     /locus_tag="PLF14_09460"
     CDS             complement(2917..3126)
                     /locus_tag="PLF14_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004762627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HOT71546.1"
                     /translation="MKVEYDFSNARPNPYAKSLKKQITININEDTVAYFKNQAKTTGI
                     PYQTLINLYLADCVNKKKKLVTTWE"
     gene            3336..5204
                     /gene="glgB"
                     /locus_tag="PLF14_09465"
     CDS             3336..5204
                     /gene="glgB"
                     /locus_tag="PLF14_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020311394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HOT71547.1"
                     /translation="MKIISDFDMHLFREGTQCQAYRMFGAHLRDNGCQFTVYAPNAKF
                     VSVVGDFNNWNLFAHNMDKSPEGVFSIFVPKAKEWDRYKYRIVTKNNKEIYKADPYAF
                     YAEHMPDTASKIAKIDDYIWNDREWMEKRNETPLYDKPVNIYEMHLGSWKRHPDNSYY
                     TYEEYAKELVSYVKNMGYTHIEFMPVMEYPFDMSWGYQCLGYFAANSRFGSPKQLKYL
                     IDCCHQNGIGVILDWVPAHFPKDSHGLIKFDGTALYEYASAKKAQHKEWGTLVFDYGK
                     KEVCSFLLSSAFFWIKEYHADGLRVDAVSSMLYLDYGRNDGEWEPNMFGGRENLEAID
                     FIKSLSTNLFREFPNIILAAEESTAWPNVTGRVSDGGLGFNFKWNMGWMNDTLSYMSK
                     DPLFRKYEHNKLTFSMMYAFSENYILPLSHDEVVHGKKSLVDKMPGYYEDKFSNLKIY
                     YAYYMSHPGKKLMFMGGEFAQFVEWRYQSGLDWMLLDYESHSKFSAYVKDLNRFYKRH
                     KAFYEAERDWVGFKWINCDAAQDSVLSYIRRDKAGRNDIFVIINFTPVDRNGYRFGVP
                     YKGKYKVLMNSSAEKYSVYGKEINMEYVSEDIPWDGMKASICVDLKGLTALYIKKI"
     gene            5215..6417
                     /locus_tag="PLF14_09470"
     CDS             5215..6417
                     /locus_tag="PLF14_09470"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOT71548.1"
                     /translation="MRKKECIALILAGGQGSRLGVLTKKVAKPAVNFGGKYRIIDFPL
                     SNCTNSNIDTVGVLTQYQPLELNSYIGSGQPWDLDRSFGGVYLLPPYMKAEAGTWYKG
                     TANAIYQNMNFIQMFNPDYVLILSGDHIYKMNYAKMVDFHIENNADATISCIKVPWDQ
                     ASRFGIMKTDKTLRITEFFEKPKNPVSNMASMGVYVFKWEVLKEYLIKDEEDPRSSND
                     FGKNVIPTMLHDGKRMFAYNFKGYWKDVGTVYSLWEANMELLHKKPGMNLFDRSWRIY
                     SKNAIQPPHYTADGAVIKDSMITEGSVIYGKVKNSVISSGVKIGKNTVIEDSVLFPSV
                     VVEDNCVIKKAIIGERSVVRQNSRIGVEDMIEGQIPSKMCSDGIVLIGSDIVVKSGSD
                     IAKNVMVE"
     gene            6426..7550
                     /gene="glgD"
                     /locus_tag="PLF14_09475"
     CDS             6426..7550
                     /gene="glgD"
                     /locus_tag="PLF14_09475"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR02092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HOT71549.1"
                     /translation="MKDALGIIFAEGTDTGFGSLTSKRSLAAVPIASRYRIIDFILSS
                     MVNSGITNVGIITQRNYKSLMDHVGTGKEWDLNRKIGGLLLIPPTPSRDIIGSDKVDL
                     LDLAGALGFLERSPQKYVVLSKSHLICNLNFTDAFEKHVNSNAIVTILYQELEPEGEI
                     SGLKNRYFLTTDENDKITQIKAYSSTNESNKISLDIYIINKDFLIYLIRDGLASGERG
                     SLSEVFARRVEHMKMIGYVARDFVARIDSIKTFYQANMVILQKEIREKLFFEPGRIYT
                     KVKDEAPSKYMKSAVVKNSLIADGCVIDGYVENSIIFRGVKVVKGARVTNSIIMQGSE
                     IQENATLEHVILDKEVIIRQDIRLTGQKSYLVLIDKGAIV"
     gene            7550..>7892
                     /locus_tag="PLF14_09480"
     CDS             7550..>7892
                     /locus_tag="PLF14_09480"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOT71550.1"
                     /translation="MRILYVSTEATPFVKTGGLADVIGALPYTVAKKGHETAIMMPYY
                     KSIRDKWRYEVKYLTSTIVTVGWRKQYAGILYLKHKGVDVYFIDNEFYFGSDNVYGYG
                     EYEAERTAFFNR"
BASE COUNT         2480 a         1350 c         1924 g         2138 t
ORIGIN      
        1 gcaggcaaca gacaaagctg ttttttggaa gaagactgca aagatagaaa gatttttcaa
       61 acttgactaa gagagggata ttttatgtta agtaatgaag caaagaagag ataccgagcc
      121 ttttgggaca ggaccgctta tgaaagatgc gtgttgttca tatccgagag gataggcaaa
      181 ggcgttgtaa tgcaaggtgc ttcaccggaa gataaatgga ccgatttgga aagacgcaca
      241 cgggatgcgc aggaatcgta tgtcaacttt aaatattatg ccgaaggttt ttccacagtg
      301 ttcaccaatt tcggaccggg cagcatggct gcatgcatag gaggagattt cgagctggct
      361 ccgtcaacaa tatggtttga ccgcaatccc atcattaaag actggtcgga agttccggaa
      421 ttgaaactgt atgaagacag cttgatgtgg cagctcatga ccgggcttac ggaaatgttg
      481 tgcaaagctt caaaatgcac atactttact tccatggcgg atatcggcgg ttcaatggat
      541 attttatcct ctttaagagg tgccgagcaa cttttatgcg acttaattga ttatcctgaa
      601 gaagttaaga aggttaacga aagaatcgaa gagttatgga agattgttta cagcaaactg
      661 tatgctatta catcccgtta tcagaacgga atgacaagct ggatgccgat atactgtgaa
      721 aagagatatt atcctttgca gtgtgatttt tgtgccatga tttctcccga catgttttta
      781 gaatttgtaa aacccaattt atcggctcag gcagaatggc tggaccattc catatatcat
      841 cttgacggaa tggatgccgt tcgtcatctg gatcaccttc tggatattcc ggctattgat
      901 gctatacaat ggagtgcagg agccggtaag ccccctttat atgacgaatg tttcttcgat
      961 atgtacaaca agatttcaag gagcggaaaa gggctggtgc ttttttccgt gccgttgacc
     1021 gaattggaaa atgttctgaa gaacatatca actaaaggtt tgtttataac catgcaggta
     1081 tcggatgaga aagaagcttt agaagctatt aaaatagcaa aaagctacgg agtaaagtaa
     1141 atatattagt gtaatatagt gtaaaataag tgttggaggg tatattatga gttttaagat
     1201 aggagttatt acggattcgt tcagatgtcc gttggagcaa gcgatagata aagctgcggc
     1261 cttgggggcg gacggcgtgc agatatatgc caccggcaga gagttggcac atgatacgcc
     1321 tgatgacagg gtaaagaaga tattgcgtct gttaaaagat gcaggtctgg aattgtccgc
     1381 tctgtgcggg gatatcggcg cattcgatga tgaaattgtc aatatcgaga gagttaagca
     1441 ttcaaaaaaa atagtagacc ttgccgtaaa gatgggaacc aaggtggtaa ccacccacat
     1501 aggagtggtg cctgaagata agaatcataa gcaatacagg acaattcata aagcatgttc
     1561 ggaactggga agatatgccg attctcaggg agtgaaattc gcaatagaga ccggtccgga
     1621 aaaagcatac atattaagaa tgcttctgga tgatttggat tgcaccggaa tagcggtaaa
     1681 cttcgacccg gccaatctga ccatgtgtgt tgacgatgac gcaagggaag cggtaaggat
     1741 actggctccg tttattgtgc atacccatgc taaggacggt attatgttaa gtaaatatgc
     1801 aaatgaccca agatacggcg aactggcaaa gaagagagca tggctggagt tgcctttagg
     1861 tgaaggagat gtcggttttc cgacctatct tgaggcttta aggaaaatag gctataaagg
     1921 gtttttgaca atagaacgtg aagtcgggga agatcccgcg aaagatattg ccaatgcggt
     1981 aactttctta cgtaaactta tataggaggc aataatgaaa atagggataa gctcatattc
     2041 atactataaa cttacaagaa aaggattgat agattgcttc ggtattattg agaaaaccaa
     2101 ggagttcgga tttgatgcca tagagttttc cggaatgccc ggcgatacat atgaagaaag
     2161 atataagatg gcaaaggata taaagaaagc ctgtgatgat tgcaatcttc tgatagctaa
     2221 ttattgcacg ggagccgacc ttgtaaaggg gtcaaactgg aatttgcaga ccgaaataga
     2281 ccgtataaaa aaagagatag atattgcagc ggtgctcggg gtaagtaaaa tgcggcatga
     2341 tgcatctttc ggtttcggag caaccgaccc caaggcacgt cctttggata tgtatcttta
     2401 caggcttgct gagggttgta aaaagttaac cgagtatgcc aaaggagcaa atatccgtac
     2461 aatgatagaa aaccacggca gatactgtca gcacagttgt gatgtgctga gcatatacag
     2521 ggcggtggat gacgataatt tcggtatatt gatagacata ggtaatttta tgtgtgtgga
     2581 tcaggacccc gttgaagccg tagcccaact ggctccttat gcattccatg tgcatgcaaa
     2641 ggattttcat ttcaagtccg gcattaacat gaatcccggc tggggatggt ttttaaccaa
     2701 gcacggcaac tatttaagag gctccattat aggacacggc gatgtcccgc tcaaagaatg
     2761 tatcaatatc cttaagatat acggatacga tgatgtgctg tcaattgaat ttgaaggtat
     2821 tgaggatgtg ctctcggcta tagagaacgg tcaggccaac ctgaaaaaat tagttgatat
     2881 gtaggatatc agtgactgtc agagcaaaag aggatattat tcccaagtcg ttacaagttt
     2941 ctttttcttg tttacacagt cagctaaata caaattgata agcgtctgat acggaatgcc
     3001 ggtagttttg gcttggtttt taaagtacgc cacagtatcc tcattaatat ttattgtaat
     3061 ctgctttttt aagctttttg cgtaggggtt aggccttgca tttgaaaaat catattcaac
     3121 cttcatagtt tgttacctca taattatttt ataattatta tttaattatt tgcaagcatt
     3181 atttttaatc ggctgccgtg tttgaaataa cggttttttt acctgtaaca ttcttttacg
     3241 tatatctgat gcagttataa ccgattattg acattacgga agtaatcata tttattaaaa
     3301 cttgtttcta tgataaaata acgaaagagg gtagcatgaa gattatttcg gattttgaca
     3361 tgcatctgtt ccgcgaagga acacaatgcc aggcatacag gatgttcgga gcacacctta
     3421 gagataacgg gtgtcagttc actgtctacg ctcccaatgc caaattcgtt tccgtagtgg
     3481 gagacttcaa caactggaat ctttttgcac acaatatgga caaatcgccg gagggtgtgt
     3541 tcagtatttt tgttccaaaa gcaaaagaat gggacagata taaatacagg attgtaacaa
     3601 agaataacaa agaaatatat aaagccgatc cgtatgcgtt ctatgccgag catatgcccg
     3661 ataccgcttc aaaaatagca aagattgacg actatatatg gaatgaccgg gaatggatgg
     3721 aaaagcgaaa tgaaacaccg ctgtatgaca aacccgttaa tatctacgag atgcatctgg
     3781 gttcctggaa gagacatccg gataattcgt attatacata tgaagaatat gcgaaggaac
     3841 ttgtaagtta tgtaaagaat atgggctata cccatataga gttcatgccg gttatggaat
     3901 atcctttcga catgtcctgg ggttatcaat gtctgggata ctttgcggct aacagcagat
     3961 tcggttcgcc taagcagctt aagtacctta ttgattgctg tcaccaaaac ggtataggag
     4021 tcattctgga ttgggtacct gctcattttc ccaaggattc gcacggcctt ataaaattcg
     4081 acggcacagc gctgtatgag tatgcgtccg ctaaaaaagc tcagcataag gagtggggca
     4141 cattggtctt tgattacggg aaaaaggaag tgtgcagttt cttgctttct tcggcattct
     4201 tctggattaa ggaatatcat gccgacggct tgagagtgga tgcggtatct tccatgcttt
     4261 atcttgacta cggcagaaat gatggagaat gggagcctaa tatgttcgga ggaagggaaa
     4321 atctggaggc catagatttc ataaaatcac tgtccaccaa tcttttcagg gagtttccca
     4381 atataatact ggctgcggag gaatctacgg catggcccaa tgtgacggga agagtaagcg
     4441 acggcggact gggattcaat tttaagtgga atatgggttg gatgaatgat accttatctt
     4501 atatgtccaa agacccgttg ttcagaaaat acgagcacaa taagttgaca ttttccatga
     4561 tgtatgcttt ttccgagaac tatattctgc ctctttcgca tgatgaggtg gtacacggaa
     4621 agaaatcgct tgtggataaa atgccgggct attatgaaga caagttttcc aatctgaaaa
     4681 tatactatgc atactatatg tcgcacccgg gaaaaaagct tatgttcatg ggaggggaat
     4741 tcgcccagtt tgtcgaatgg agatatcagt cgggactgga ttggatgctg ctggactatg
     4801 agtcacacag taagttttct gcatatgtta aagacttaaa cagattttac aaaaggcata
     4861 aggcctttta tgaagcggaa agagactggg taggttttaa atggataaat tgtgatgccg
     4921 ctcaggacag tgtactttcg tatattcgca gagataaggc gggaagaaac gatatttttg
     4981 ttattattaa tttcacacct gtagaccgta acggataccg tttcggtgta ccgtataagg
     5041 gtaagtataa agtgttgatg aactcttcgg ctgagaagta ttccgtatac ggtaaagaga
     5101 ttaacatgga atatgtttcc gaagatattc cctgggacgg tatgaaagcc agtatatgcg
     5161 ttgatctgaa aggattgact gcattgtata taaaaaagat ataggaggct acgtatgcga
     5221 aaaaaagaat gtatagcatt gatacttgca ggcggacagg gcagccgctt aggggtgctt
     5281 accaagaagg ttgccaaacc tgcggtaaat ttcggcggga aatacagaat tattgatttt
     5341 ccgctcagta actgcacaaa ctccaatata gatacggtgg gtgttctgac tcagtatcag
     5401 ccgttggaac tcaattccta tatcggaagc gggcagccgt gggatttgga cagaagtttc
     5461 ggaggggtat atctgctgcc cccgtacatg aaagcggagg cgggaacatg gtataaagga
     5521 acggcaaatg ccatatacca gaacatgaat ttcattcaga tgtttaatcc cgattacgta
     5581 ctgatactgt caggggatca tatatacaaa atgaattatg ccaaaatggt ggattttcat
     5641 attgaaaaca atgccgatgc tacaatttcc tgtataaaag ttccgtggga tcaggcaagc
     5701 cgtttcggta ttatgaaaac cgataaaacg ctgcgtataa cggaattctt cgagaaaccc
     5761 aagaatccgg taagcaatat ggcatccatg ggtgtgtatg tattcaagtg ggaagttctt
     5821 aaagaatatc ttatcaaaga cgaagaagat ccccggtctt caaatgattt cggtaaaaac
     5881 gtcataccta caatgcttca tgacggaaaa aggatgtttg catataactt caaaggctac
     5941 tggaaggatg taggtaccgt ttacagcctg tgggaagcca atatggaact tttacacaag
     6001 aaacccggca tgaatctgtt tgacagaagt tggagaatct actcaaagaa cgccattcag
     6061 cctcctcact atactgcgga cggagcggtt ataaaagaca gtatgataac agaaggaagc
     6121 gtcatatacg gtaaagtcaa aaacagtgtt atttccagcg gtgtaaaaat aggaaagaat
     6181 accgtaatag aagactcggt actgtttccg tctgttgtgg tagaagataa ttgtgtaatt
     6241 aaaaaggcta ttatagggga gcggtcggtg gtcagacaaa acagtagaat aggtgtggaa
     6301 gacatgattg aaggacagat tccttcaaaa atgtgttcag acggaattgt gcttatcgga
     6361 tcggatatcg ttgttaagtc cggatccgat atagcaaaga atgtaatggt ggaataggtg
     6421 atgatatgaa agatgcatta ggaattattt ttgcggaagg tacggacacg ggattcggtt
     6481 ccctgacatc taagcgatct ttggcggcag tacccattgc gtcgcgatac aggataatag
     6541 atttcatatt gtcttccatg gtcaactcgg gaataaccaa tgtcggtatt ataactcaaa
     6601 gaaactataa atcgcttatg gatcatgtgg gaaccggtaa ggaatgggat cttaacagaa
     6661 agataggagg tctgcttctg attccgccta ctccctcaag agatattata ggcagcgata
     6721 aagtggattt gctggatctg gcaggcgcat tggggtttct ggaacggtca cctcaaaagt
     6781 atgtggtgct atccaaaagc catctgatat gcaatctcaa ttttaccgat gcttttgaga
     6841 agcatgtcaa ttcaaatgcc atagtaacca tcctgtatca ggaactggaa ccggaaggtg
     6901 agataagcgg tttgaaaaac aggtatttcc tgactaccga tgagaatgac aaaataacac
     6961 agataaaagc ttattcatcc acaaatgaaa gcaataaaat ttctttggac atatatatta
     7021 taaacaagga ttttcttatt tatctcataa gagacggtct tgcgtcgggt gaaagaggaa
     7081 gtttaagtga agtatttgcc agaagagtgg aacacatgaa gatgataggg tatgtcgcca
     7141 gggattttgt ggcaagaata gactccataa agaccttcta tcaggccaac atggtcattc
     7201 tgcaaaagga aataagggag aaactgttct tcgaaccggg acgtatatat accaaggtaa
     7261 aagacgaagc gccgtccaaa tatatgaaat ccgcggttgt caagaactcg ctgattgccg
     7321 acggatgtgt catagacggt tatgtggaaa acagtataat attcagagga gttaaagttg
     7381 taaagggagc cagagtaacc aattcaatta tcatgcaggg ttccgagata caggaaaacg
     7441 caacgctgga gcatgttatt ctggataagg aagtaataat aaggcaggat atacgtctga
     7501 ccggacagaa atcctacctg gtactgattg ataaaggagc tattgtataa tgagaatttt
     7561 atatgtatca accgaagcaa caccttttgt aaagaccgga gggcttgcgg atgtaatcgg
     7621 agcattaccg tataccgttg caaaaaaagg tcacgaaact gcaattatga tgccgtacta
     7681 caaaagtata agggataaat ggagatatga ggttaaatat ctcacaagca ccattgtgac
     7741 tgtaggatgg cggaaacagt atgcgggaat actgtatctt aaacacaaag gagtggatgt
     7801 atatttcatt gacaacgagt tttatttcgg ctcggataat gtctacggtt acggagaata
     7861 tgaggcggag aggacggcat tctttaacag ag
//
LOCUS       DAOSOM010000063         7862 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_9212_length_7862_cov_5.756629, whole genome shotgun sequence.
ACCESSION   DAOSOM010000063 DAOSOM010000000
VERSION     DAOSOM010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7862
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9212_length_7862_cov_5.756629"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(352..558)
                     /locus_tag="PLF14_09485"
     CDS             complement(352..558)
                     /locus_tag="PLF14_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71551.1"
                     /translation="MLDRLAVLFGIPTENLLHEIISTKHISFPFRASKMNEKNLEAIS
                     AINRIVFNLRFMRKCQEFGKKAAE"
     gene            complement(885..2174)
                     /locus_tag="PLF14_09490"
     CDS             complement(885..2174)
                     /locus_tag="PLF14_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017698179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOT71552.1"
                     /translation="MSKVFDKTKYKSRIIDSLVEKNLRIFSAVCIEGPKWCGKTWTSS
                     YHANSDFFVGDPSGNFSNRMLAETEPYTVLQGDKPRLIDEWQEVPTLWDAVRSFVDES
                     GEKGQIILTGSSTPVSKGILHSGTGRIRNIRMNTMSLYESGDSDGLISLKDLCENKFE
                     PKLLEEAKLERLAYLIVRGGWPGNIDASFDDCAELAIGYMENIAKTDLKKLDRDIDYN
                     EHKAKLILKSLARNESTTVSNQSILRDIVDNDADSISKNTLAKYLNAFDRMFLFNNQE
                     PFSPNIRSSLRVKQMEKRHFSDPAMACAMLKLTPKKMMNDLNTFGFMFEALVERDLSI
                     YAQAMNAKLFHYQDYKGNEIDAVMELDDGNWCAFEIKLGLNKAEEGAENLIKVCNAIV
                     DNGGKEPLLKCVIYGAGNMAYRNANGVYILPLTSLKQ"
     gene            complement(2391..2466)
                     /locus_tag="PLF14_09495"
     tRNA            complement(2391..2466)
                     /locus_tag="PLF14_09495"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2431..2433),aa:Trp,seq:cca)
     gene            complement(2539..3213)
                     /locus_tag="PLF14_09500"
     CDS             complement(2539..3213)
                     /locus_tag="PLF14_09500"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOT71553.1"
                     /translation="MGLNEKIIKAIVFDMDGVLIDSEQVMSKAAILALKEFGVNAQPY
                     DFIPFMGQGENSFIGNVARKYNVEYQYAMKYRAYEIYDELVSDEAKLFPNIKETITTL
                     KKRGYRISLASGADMMKVSSNIKAIGMEESDFEAFVTGNDVTRNKPEPDIFLLAAEKM
                     KINPAYCLVCEDTISGIKAAKAAGMQCLGITSSLQSEVLKNSGADETSDSIHILLDIL
                     MPLSKR"
     gene            complement(3226..3576)
                     /locus_tag="PLF14_09505"
     CDS             complement(3226..3576)
                     /locus_tag="PLF14_09505"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOT71554.1"
                     /translation="MLFRKQDQKVSYRENMRDGQGTIMFNELLPKQFLNGKGRLFSLI
                     TVEPGCSIGFHVHENESETYYVIEGSLVYNDNNEKEYVIQEGDCVYNGNMRGHSVRNE
                     GKSKAVVLALILFM"
     gene            complement(3687..5519)
                     /gene="dxs"
                     /locus_tag="PLF14_09510"
     CDS             complement(3687..5519)
                     /gene="dxs"
                     /locus_tag="PLF14_09510"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOT71555.1"
                     /translation="MELLDRIKVSSDVKDIKKEDIDELCRQIRTFLVEVVTGNGGHLA
                     SNLGVVELTVALHRCLDMEKDHIIFDVGHQSYVHKILTGRKDSLATMGSIDGLGCFPD
                     EEESRYDSFNTGHASTSVSAALGMEKALRMSGSDGKVVAVLGDGALGGGMVYEALNNA
                     KDLDSDILIILNDNEMSISRNVGAMSRHLSKLKLRPSYIDTNLRIKHIVEKTPGGKGF
                     IRLIQKTKRALKSFVGQKMFFEDLDIRYLGPVDGHDEHQLESIIKEALNVKGPKLLHV
                     VTTKGKGYEKAERDPVKFHGLKNSYSKSRRLPENIMYNQLFGKLLTEMAEKDKNIVCV
                     CPAMTEGSGLSEFCKVHKDRYFDVGICEQHAVTFSAGLARDKIKPVCVVYSSFLQRAY
                     DQILHDIGLTNKNVVLCVDRAGVVDAYGKTHQGIYDIAYLSTIPNMRIFAPESEEDME
                     KLLYKAIYDIEGCVAVRYPYDQIHNDIKNLFVDVSEKREISLYSKNGDKAILCYGRMV
                     AVAVEAADIAGADVVKLTDIYPLDYESLYEVLGKYKYVVSAEDVIYNGSVGQRLQSWL
                     YENNKNDFKLICLNIDEDCEMKGCVEDILEENGMSKDAIARIIS"
     gene            complement(5523..6392)
                     /locus_tag="PLF14_09515"
     CDS             complement(5523..6392)
                     /locus_tag="PLF14_09515"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOT71556.1"
                     /translation="MTNLNTVMKKYGEQVTLFINQRINKSNAPDIIKEACLYSVNAGG
                     KRIRPVLFLMTLDALNAEITEKHIAYASAIEMIHTYSLIHDDLPAMDNDEYRRGQLTS
                     HIKFGEAYAILAGDALLNLAYETMLDYAVNAKDIAAARNIAEAAGYQGMVGGQVLDMK
                     KPDTVNDILAMYSLKTGALIKTSVVSAGIIAGCNKRTLKRLADYAEGIGVAFQLRDDY
                     LDKTDINEKTGKPKDSDRKNEKATYISKRGIAANQNKLQKYTKKALTALQPYGDSYNN
                     LRSLAEFLGDRMK"
     gene            complement(6389..6595)
                     /gene="xseB"
                     /locus_tag="PLF14_09520"
     CDS             complement(6389..6595)
                     /gene="xseB"
                     /locus_tag="PLF14_09520"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010530943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOT71557.1"
                     /translation="MSNKDFNTLYRELEKKVELLEKGELPLEEAVKTYTEGQELIKLL
                     NEKLNKAREQMVVTDKSEQKEPEK"
     gene            complement(6588..7793)
                     /gene="xseA"
                     /locus_tag="PLF14_09525"
     CDS             complement(6588..7793)
                     /gene="xseA"
                     /locus_tag="PLF14_09525"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOT71558.1"
                     /translation="MEKALTVSQLNEYIKQLVSSDEVLESLHVEGEISNCTIHYSGHI
                     YFSIKDDKCAIRCVMFSGYTKSLKFEPADGVKVTVYGYLSFYEKTGQCQIVVTNMLES
                     GTGDLFIKFENLKKKLEKEGLFDTGKKKPLPFLPETIGVITSSTGAVLQDIRNILNGR
                     FPNYGFILYPATVQGVDAHKTVIKGLEYFNKNKNADVIIIARGGGSVEDLWPFNEEEL
                     AYAIHNSNIPVISAIGHETDFTIADFVADARAATPTAAAQMVIPEKKRLERDVLSQNE
                     SLEFSYAKLINNLTERVSDLAKRPVMKRPEEYTNILAMNLDNLTGRFFTFSKQYLDNV
                     AYKLSTLIHKADAMNPLAVLSRGYSITTDKKGHVIKSADNLNKNDVILTTLHAGKIRS
                     VVEKAVKDE"
BASE COUNT         2118 a         1858 c         1211 g         2675 t
ORIGIN      
        1 atgttaaata cctccaaaat tattctattt tgttttggat gcaatataat cttcgggtaa
       61 ataccctcac aatttgctaa ccttatcacc cttcatcagc gtaagtgata tactgcgtta
      121 gtattattat gccggatttt ctgtcttaaa aatgcaaatt gttttttttt gctctatttt
      181 gcaaatgaat atacgtattt tacacataga catcggaata tatgttatgg gtactatatt
      241 atctattcat ttctatatac ttaaaattat aaagtcataa tctttactgt tttaccggtt
      301 tagaggatag agttgaatct tacacctact gaaaatattc aaaaaaaaca atcattcagc
      361 agcttttttt ccaaactcct gacattttct cataaatctt aaattaaaaa caatccgatt
      421 tatggcgctg attgcttcta aatttttctc gttcatcttg cttgctctga aaggaaatga
      481 aatatgcttt gttgaaatta tttcatgcaa gagattttct gtcggtattc cgaataatac
      541 agccaacctg tccagcatat cagatgttat agactgctcc cctttctcaa tctttgacat
      601 taaactttaa tcaacattca aatacttcgc aatactgctt tgcttaaaac cgctttgttt
      661 tcttaatttc cttatgttct taccgataat caacattgcg ttttccataa caatctctcc
      721 tgtctaccat tatatactta actaatgtta tattttcaat agacaaacac caataattat
      781 aaaaaacttt gctgactcca ttaaacttca cctttaagaa caataatctt catgtattgc
      841 taaaagaaaa cggtattttc aatacccggt tttacaactc gggattattg cttcaaagaa
      901 gttagaggaa gtatgtaaac tccgttagca tttctatatg ccatattacc ggctccgtat
      961 attacgcatt ttaataacgg ttcttttccg ccgttatcaa ctattgcatt acatacttta
     1021 atcagatttt cagcgccttc ttccgcctta ttaagaccta atttaatttc aaaagcacac
     1081 caattgccgt catccaattc cataacggca tcaatttcat ttcctttata gtcttgataa
     1141 tgaaacaact ttgcattcat agcttgtgca tatattgata aatctctttc taccaaagct
     1201 tcaaacataa aaccgaaagt gtttaaatca ttcatcattt tcttaggtgt caacttcagc
     1261 attgcacatg ccattgcagg atcggaaaaa tgtctttttt ccatttgttt gactcttaat
     1321 gatgacctta tattaggtga aaacggctct tgattgttaa acagaaacat tctgtcaaac
     1381 gcattcaaat acttagccaa tgtattttta ctgatagaat cggcgtcatt atcaacaata
     1441 tctctcaaaa tgctttgatt ggatactgtt gttgattcat ttctcgctaa cgatttaaga
     1501 attaattttg ctttatgttc attataatca atatcccggt ccaacttttt caggtctgtt
     1561 ttcgctatat tttccatata acctatagca agttcagcac agtcatcaaa agaagcatcg
     1621 atatttcccg gccaaccgcc ccgaactatt aagtatgcca gcctttcaag ctttgcttct
     1681 tctaatagct tcggctcaaa tttgttttcg cataaatctt ttaatgatat taaaccgtcg
     1741 gaatcacctg actcatacag agacattgta ttcatcctga tgtttctaat cctacccgta
     1801 ccgctatgta gaataccttt gcttacggga gttgatgacc cggtcagaat aatctgtcct
     1861 ttttcaccgc tttcatctac aaaagatctt accgcatccc atagtgtcgg tacttcctgc
     1921 cattcatcaa tcaatcgcgg cttatcgcct tgcagtactg tataaggttc tgtttccgct
     1981 aacattctat tagagaaatt acctgacgga tcgccaacaa aaaaatcact gttagcatga
     2041 tatgaggatg tccatgtttt tccgcaccat ttaggaccct caatacacac tgcactgaat
     2101 attcttaaat ttttctcgac caatgaatca attatcctgg atttgtattt tgttttgtca
     2161 aatactttac tcattaattg tcacacctcg cagtttctga caacagtcta tcacaaagat
     2221 aagcactaat caataagaac tgaccaacat ttccggctaa aactgaccaa catttccaac
     2281 taaaactgac cggtatttcg agcaactcat gcaaagtgtg cttgtcaagt ataacttgta
     2341 ataataaaat acgacacatc tcataacgaa atatgtcgta ttcactgttc tggcagggaa
     2401 gacaggattt gaacccgcaa cttacggttt tggagaccgc cgctctaccg attgaactac
     2461 ttccctacgc agcagaagtg attatataaa tttaatatag ttatgtcaag agcaagattg
     2521 cttgagggct gtttactcct accgcttgct taacggcatc aatatatcca aaagtatgtg
     2581 aatactgtcc gatgtttcgt ccgcaccgct gtttttaagc acttcgctct gtaacgaact
     2641 tgtaataccc agacattgca tgcctgctgc ttttgccgct ttaattccgc ttatggtatc
     2701 ttcacaaacc agacagtatg cgggattaat cttcattttt tcagcggcca acaggaaaat
     2761 atccggttca ggcttatttc ttgtcacatc attaccggta acaaatgctt caaaatccga
     2821 ctcttccatc ccgatagcct taatgtttga actgaccttc atcatatcgg cacccgatgc
     2881 caatgatatt ctgtatcctc ttttcttaag tgttgtaatt gtttccttga tattcggaaa
     2941 cagctttgct tcatcgctga caagttcatc atatatctca tatgctctgt atttcattgc
     3001 atactgatat tccacattgt actttcttgc aacatttcct ataaaactgt tctccccctg
     3061 ccccataaac ggtataaaat cgtaaggttg cgcattcact ccgaattcct tgagagccag
     3121 aattgccgcc ttgctcatta cctgctcgga atctatcaac actccgtcca tatcaaatac
     3181 tatcgcttta ataatttttt cgtttaaacc catactaccc ctttactaca taaaaagaat
     3241 aagagccagc accacggctt tgctcttacc ctcgtttctc acactgtgac cgcgcatatt
     3301 cccgttatac acgcaatcgc cttcctgtat tacatattcc ttctcattat tgtcgttgta
     3361 tactaaagaa ccttcaatta cataatatgt ttccgattcg ttttcatgaa catgaaagcc
     3421 tatcgaacaa ccgggttcaa cggtaatcaa agaaaaaaga cggccttttc cgtttaagaa
     3481 ctgtttaggc agtaattcat taaacatgat agtaccctgt ccgtctctca tgttctccct
     3541 gtatgacact ttttgatctt gctttctaaa cagcatataa agattatcct cttacataca
     3601 tcaatttctt tcctattata cattcaaata tatactgatt gcaatgcctt ttgcctgcat
     3661 gacaatctgt acatccatta tttttgctaa ctgataatcc ttgcaattgc atctttagac
     3721 atcccgtttt cttccagtat atcttcaaca caccctttca tctcacaatc ctcatcgata
     3781 tttaagcata tcagtttaaa gtcattcttg ttgttctcat atagccatga ttgcagcctt
     3841 tgacctacag acccgttgta tatcacatct tcggcagata caacatactt atatttcccc
     3901 aaaacctcat acagactttc ataatccaaa ggatatatat cggttagttt aaccacatct
     3961 gcacctgcaa tgtccgccgc ttccacggca acagcaacca tcctgccgta acacagtatt
     4021 gctttgtcac cgtttttact atataaagaa atctctcttt tttcggacac atcaacaaag
     4081 agattcttaa tatcattatg aatctgatca tacggatatc ttacggcaac acatccttca
     4141 atgtcatata tggctttata taacagtttt tccatatcct cttccgattc cggtgcaaat
     4201 attctcatat tgggaattgt ggacagatat gcaatatcat aaatcccctg atgggtctta
     4261 ccgtatgcat ccacaacccc cgccctgtcc acacacaaca ccacattttt gtttgtcagc
     4321 cctatatcat gaagtatctg atcgtatgcc ctttgtaaaa aagatgagta tacaacacat
     4381 accggcttta ttttgtctct ggcgagaccg gcactgaaag taaccgcatg ctgttcgcaa
     4441 atacctacat caaaatatct gtccttatgt actttacaaa actcgcttaa acccgatcct
     4501 tcggtcattg cgggacatac acaaactata ttcttatctt tttcggccat ttcggtcaga
     4561 agctttccga aaagttgatt atacataata ttttcaggca gtcttctgct ttttgaataa
     4621 ctgtttttaa gaccgtgaaa cttaacggga tctctttccg ccttttcata ccccttacct
     4681 ttggttgtta ccacatgcag cagtttaggc ccctttacat tcaatgcctc ctttattata
     4741 gactccagct gatgctcgtc atgtccgtca acgggaccca gatatcggat gtccaaatcc
     4801 tcgaagaaca tcttctgccc tacaaaagac tttaatgctc tttttgtttt ttgtatcaac
     4861 cgtataaatc ccttgccgcc cggtgttttt tccacaatat gcttgattct aaggttagtg
     4921 tcaatatatg aaggacgtaa cttgagtttt gaaagatgtc tcgacattgc ccccacattc
     4981 ctgcttatgg acatttcatt gtcgttaaga attatgagaa tatcggaatc caaatcttta
     5041 gcgttgttca aggcctcgta taccattccg ccgcccaaag ctccgtcacc taaaaccgcc
     5101 actactttgc cgtccgaacc gctcattctt aaggcttttt ccatacctaa agcggcggag
     5161 accgatgtgc ttgcatgtcc ggtattgaaa gaatcgtacc tgctttcctc ctcatcggga
     5221 aagcaaccca atccgtcgat gcttcccatt gttgccagac tatcttttct tccggtcaga
     5281 attttatgaa cataactttg atgccccaca tcgaaaatta tgtggtcttt ttccatatcc
     5341 agacatctgt gcagcgccac cgtcagttcc accactccca gattacttgc aaggtgtcct
     5401 ccgttaccgg tcacaacctc cacaagaaaa gtccttatct gtctgcataa ctcatctata
     5461 tcctcttttt tgatatcctt cacatcggaa gataccttta ttctgtccag aagttccata
     5521 cactatttca tcctgtcacc taaaaactcc gctaatgagc gtaagttatt atagctgtct
     5581 ccgtaaggct gtaaagccgt taatgctttt ttcgtatact tttgcagttt attttggttt
     5641 gccgcaatcc ctcttttgga gatgtatgtg gctttttcat tttttctgtc actgtctttg
     5701 ggttttccgg ttttttcgtt aatatcggtt ttatccagat aatcatctct taactggaag
     5761 gctacaccga taccttcggc ataatcagcc agtcttttca atgttctttt gttgcatccg
     5821 gctattattc cggctgatac caccgatgtt tttataaggg cacccgtttt caacgaatac
     5881 atagcaagaa tgtcattgac ggtatcgggc tttttcatat ccagaacctg cccgcctacc
     5941 attccctgat atccggccgc ttcggctatg tttcttgcgg ctgcaatatc ctttgcattg
     6001 actgcataat ccagcatcgt ttcatatgcc aggttcaata aagcgtctcc ggctaaaata
     6061 gcgtatgcct caccgaattt gatatgactg gtcaactgcc ctcttctgta ctcatcatta
     6121 tccattgccg gcaaatcgtc gtgtatcaac gaatatgtgt gtatcatctc aatagccgat
     6181 gcatatgcta tatgcttttc ggtaatttcc gcattgagag catccaaggt cataagaaac
     6241 agaaccggtc ggattctttt accgccggca tttacactgt acaggcaggc ttcctttatt
     6301 atgtcgggcg cattgctttt gtttatcctc tgattgataa acagagtaac ctgttccccg
     6361 tattttttca ttacggtatt caagtttgtc atttttccgg ctccttttgt tcggatttat
     6421 cggtcactac catttgttca cgggctttgt taagtttttc gttgagcagt tttatcaact
     6481 cctgcccttc ggtataggtc tttaccgcct cttccaacgg cagttcccct ttttccagta
     6541 actccacctt cttttccagt tccctgtaga gcgtattgaa atccttatta ctcatctttt
     6601 accgcctttt ccaccacact ccttatcttt cccgcatgaa gagtggttaa tataacatca
     6661 tttttattca gattgtccgc actttttata acatgtcctt ttttatcggt cgttatactg
     6721 tatcccctcg ataacaccgc taaaggattc atggcatccg ctttatgtat taatgtactt
     6781 aatttatatg caacattgtc cagatattgc tttgagaagg tgaaaaacct gcctgtaagg
     6841 ttatccagat tcatagccaa tatattggta tattcctccg gtcgcttcat aaccggtctt
     6901 tttgccaaat cggaaactct ttcagtaaga ttatttatga gtttcgcata ggaaaattcc
     6961 agactttcat tttgcgaaag aacatctctt tccagacgtt ttttctccgg gataaccatc
     7021 tgagcggctg ccgtaggtgt tgcggctctg gcatccgcca caaagtccgc aatagtgaaa
     7081 tccgtttcat gacctattgc ggatatgacg ggaatgttgc tgttatgtat tgcatatgcc
     7141 aactcttcct cattgaacgg ccaaaggtct tcaaccgaac cgccgcctct tgctattatt
     7201 atgacatcgg catttttatt cttattaaaa tattccagtc ctttaattac ggttttatgt
     7261 gcatcaactc cctgaacggt cgcgggatac aaaatgaatc cgtaattagg aaaccttccg
     7321 ttcagtatgt tccttatatc ctgcaaaacc gctccggtac ttgaggtaat tacacctatg
     7381 gtttccggca aaaacggcaa cggtttcttt ttccctgtat caaaaagacc ttctttctcc
     7441 agtttcttct tgagattctc gaatttgatg aataaatcac ctgtcccgct ttctaacata
     7501 ttggtaacaa caatctgaca ctgcccggtt ttttcataaa aagaaaggta tccgtaaacc
     7561 gtaaccttca caccgtccgc cggttcaaac ttgaggcttt tggtatatcc ggaaaacatt
     7621 acacatcgta tagcacattt atcatccttt atggaaaagt atatatgacc ggaatagtga
     7681 atggtgcagt tggatatttc accttccaca tgcagacttt ccaatacttc atcggaagaa
     7741 acaagttgtt tgatatactc attgagctga gatacggtca gggctttttc catcactgct
     7801 ctccgttttg aatatttgcc agcaccgcat tgatatattt tccggcatca tcactgccgt
     7861 ag
//
LOCUS       DAOSOM010000064         7442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_9953_length_7442_cov_4.966021, whole genome shotgun sequence.
ACCESSION   DAOSOM010000064 DAOSOM010000000
VERSION     DAOSOM010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7442
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9953_length_7442_cov_4.966021"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="PLF14_09530"
     CDS             complement(<1..783)
                     /locus_tag="PLF14_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71559.1"
                     /translation="MKKVICFLLIVMLVFLPSCKTVSDTDSGFDNNTKTGKVFTGDIN
                     DKKFTRFRLSDHCSYSQFIETYKGIYYLVGHTVYFSSDGIAKYVKLCSKPDCMHMDDY
                     NCNAYIKTNLIAYYDGYIYCVALWEGNYWLFRMNMDGTDHVPIKLISTMQERYAGFFE
                     HGYLYYYKGCIGRIGNTNTVIYRTPVFDNSEGTVVVDAKDKGIGVDEIMLFYPQDEHL
                     YIYTYSPDKKYSVCRYSMENGQWSDFVNTEWCYENVLTGTDKV"
     gene            complement(879..2039)
                     /locus_tag="PLF14_09535"
     CDS             complement(879..2039)
                     /locus_tag="PLF14_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71560.1"
                     /translation="MKKVICFLLIVMLVFLPSCKTVSDTDSGFDNNTKTGKVFTGDIN
                     DKKFTRFRQSDYCSHCQFVQTYKGCYYCKNRFIYFSSDGISKYMKLCSKPDCLHNDRD
                     ENCNAYANTHLIAYYDRYIYYISVCQNSYWLCRMSMDGTDHKNIKPIASAKEGCTAFF
                     EHGYLYYYKGFLGLLGNVNTAIYKTAVFDNSESVVVLDVKDKGIAVDDFAWFYPQDGY
                     IYIYSYSPETEYSLCRYSMETGKWSDFVNTEMCCQSVFTEENKVYWYLFDDGFYEYTY
                     ETGITKRVADAVAEGFYAISYNDEYIYAFQYSDKRDSGVAPMFYIYDRQYKLIDSVLI
                     DIEMEDYILSVYLTELEDYVLLTSSLINKPPDYYLLKSEFGSGDIKVRKIGE"
     gene            2192..2824
                     /locus_tag="PLF14_09540"
     CDS             2192..2824
                     /locus_tag="PLF14_09540"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOT71561.1"
                     /translation="MLIKKNQKVLMIGDSVTDCNRRDQNSAYLGHAYPHIVASKFYLE
                     HPDMDVEVINKGISGDTSKMLVDRWQVDVVDMKPDILTILIGINDVWRHYDTPDVTRR
                     VSKEDYIKNMRFMIESMIDKTKKIIILSPFYLEENMSKPMKAQAHDYIVALRALVKEY
                     DVLFIDLQKEFEKLMKKYTYDQLTFKTDKIHPTMFGQYVIATLIYDTLTK"
     gene            complement(2828..3757)
                     /gene="cysK"
                     /locus_tag="PLF14_09545"
     CDS             complement(2828..3757)
                     /gene="cysK"
                     /locus_tag="PLF14_09545"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015616290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOT71562.1"
                     /translation="MTVFKEILDTIGNTPLVELCAIKEKYKLNANIFAKMESFNPGGS
                     VKDRTAYSLIRGLQKDREYIDAVKNNRKISIVEATSGNTGIALAMICAKMGYNLIICM
                     PDNASKERVLLLKAYGAEVVLTDAAYGMKGAIDKSLEYTDAFKLEQFKNPYNPLIHEQ
                     TTAKEIFDALKGKLDIFVAAVGTGGTLTGCARYFKRQKNVHIVAVEPFESQAMAGKQP
                     GAHGISGIGAGFIPETLDMKLVDEIFPVKTESAYEWTGNLARTEGILCGVSSGAALAA
                     AVEIAKRGENKGKNIALILPDTGERYLSTPLFE"
     gene            complement(3744..4802)
                     /gene="mnmA"
                     /locus_tag="PLF14_09550"
     CDS             complement(3744..4802)
                     /gene="mnmA"
                     /locus_tag="PLF14_09550"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOT71563.1"
                     /translation="MAIDRYKGSVLIGLSGGVDSAVSAYLLKEKGYKVYGITYILSDN
                     QLKSAEDAKAIAKTLDIPLYIEDKKKEFNDTVVSYFVDSYICGETPNPCVLCNSSFKL
                     KNLTDKADSLSIDYVATGHYANIQYDKVSDRYILRTSENKEKDQSYFLYRLSRQQLAR
                     LLLPISDMNKEQVRDIAAKIRLTVKDKKDSQDICFIKDIDYTDLIKEKRPGKYKECTF
                     IDKEGNVLGKGANHIHFTTGQRRGLNKGFNQRMYVISKNAKSNTVTLGPVEDLYTKVL
                     TVKDIHLIQPDKAEDPLRVKVKIRNTMAAVDAVIEKTDYGIKVVFDQPVRAAAKGQSA
                     VFYLYDQVIGGGIIDDCI"
     gene            complement(4792..5508)
                     /locus_tag="PLF14_09555"
     CDS             complement(4792..5508)
                     /locus_tag="PLF14_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71564.1"
                     /translation="MKNKVWIIIIAVLIILSGGVSGYFTARAVNHFMDEMEQPVEKPD
                     VQKGTFSMYEKMGISSDELGAYSTPEEAIMNINKPIKMENVGFTSEEITAMFNISKND
                     LKAFYAESALFNIQPDNSLHIDVYLDGDALKGVTYLPDMALSVITKTKFTLHTTVEGV
                     QDGKLMVKINDIYTGEVHVMNFVNLFNLGSEVRGVFSEYIGKTVSLDISVLKDLKSFA
                     VSDGLVFIDATFQSGGAGGN"
     gene            complement(5537..6901)
                     /gene="uxaC"
                     /locus_tag="PLF14_09560"
     CDS             complement(5537..6901)
                     /gene="uxaC"
                     /locus_tag="PLF14_09560"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HOT71565.1"
                     /translation="MNKTKYIYSKAGQLLYDEVARDLPIADYHCHLSPKEIYEDKPFD
                     NIGVMWLEKDHYKWRLMRAFGIEEQYITGKAGWKDKFFAYCKAIEHSPGNPLYHWSAM
                     ELKQYFDTDTSISEENAQTIWDKANAVIKQKKLSPRKLIASSNVIYIATTDDIADDLQ
                     YHKLIKADKSFKTVVAPTFRTDNVLLINKPDYAEYIKRLEKVSGTNITDLKSLCEAVE
                     KRLDYFAKQGCSLSDVGIQYEADADFDYATADRAFKHALKGKEVEMGDYKKFLGYMFV
                     YLSGQYKKRDITQQIHTGVYRNTNTRLFESIGPDCGCDISSNSLDAVSFARLLDEMDK
                     RNQLAKTVVYILNPASYYQALTACGSFRDVLFGAAWWHMDHYEGIAENIKNVASLSCL
                     GAGLGMLTDSRSFLSYARHDYYRRILCSVIGNWVDFDEYPIECAKKLIYNLCVGNAKE
                     RFMK"
     gene            complement(6894..>7442)
                     /gene="udk"
                     /locus_tag="PLF14_09565"
     CDS             complement(6894..>7442)
                     /gene="udk"
                     /locus_tag="PLF14_09565"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOT71566.1"
                     /translation="RFGKDISVIRHDDYYRSQDDISFEERVKTNYDHPDSFETDLLIE
                     HLKQLKNGHSIECPVYSFSEHTRTKETRKIDPTKVIIVEGILIFQNPELLNMLDIRIF
                     VDTDPDVRILRRILRDVKERGRSLDSVVTQYLSTVKPMHEQFVEPSKRNADIIVLEGG
                     YNLVALDMIIQRIQGHVEATDE"
BASE COUNT         2164 a         1605 c         1252 g         2421 t
ORIGIN      
        1 cactttatcg gtaccggtta atacattttc ataacaccat tcggtattta caaaatcact
       61 ccattgtccg ttttccattg aatatctgca tacggaatat ttcttatccg gagaataagt
      121 atatatgtat aaatgctcgt cctgcggata aaacaacatt atttcatcca cacctatacc
      181 tttgtctttg gcatctacca ccaccgtacc ttcactgttg tcaaaaaccg gtgtcctata
      241 tatcacggta tttgtgttcc ctatacgacc gatacatcct ttgtagtagt ataagtaacc
      301 gtgttcaaaa aaacctgcat atctttcctg catagtggaa ataagtttta taggaacatg
      361 gtccgttcca tccatattca tcctgaataa ccagtaatta ccttcccaaa gagcaacaca
      421 gtaaatgtag ccgtcgtaat atgcaatcaa atttgtcttt atgtaagcat tacaattata
      481 atcatccatg tgcatgcaat cgggttttga acacaacttt acatattttg caataccgtc
      541 ggaactgaaa tagacagtgt gtcccacaag ataataaata cctttgtatg tttcaataaa
      601 ctgtgaatag gaacaatgat ccgataaccg gaaacgtgta aattttttgt cgttaatatc
      661 gccggtaaaa accttacctg tttttgtgtt gttatcaaat ccgctatccg tatcggaaac
      721 ggtcttacag gaaggtaaaa ataccagcat tactattaat aaaaaacata taactttttt
      781 cattgttaat aatatatttc cgcataactg ttattatcaa gtaaattata ctacagtatt
      841 tttgcgataa taattaactt ttactgaata cccgtctgtc attcccctat ttttctcact
      901 tttatatccc cgctgccgaa ctcggatttt aacaggtaat agtcgggcgg tttatttatc
      961 aatgaggatg tcaagagaac atagtcctcc aattccgtca aatataccga tagaatataa
     1021 tcctccattt caatatcaat caatacactg tctatcagtt tgtactgcct gtcatagatg
     1081 taaaacatag gagcaacacc tgaatctctt ttgtcggaat attgaaatgc atatatatat
     1141 tcatcattat atgaaattgc ataaaaacct tcagcaacgg catcagccac acgcttggtt
     1201 atacccgttt cataggtgta ttcataaaaa ccgtcatcaa aaagatacca atatacctta
     1261 ttttcctccg taaatacact ctggcaacac atttcagtat ttacaaaatc actccatttt
     1321 cccgtttcca ttgaatacct gcacaatgaa tattccgttt caggcgagta agagtatata
     1381 tatatgtatc cgtcttgagg ataaaaccag gcaaaatcat ccacagcaat gcctttatcc
     1441 ttaacatcaa gcactactac actttcactg ttatcaaaaa ccgctgtctt gtatatggcg
     1501 gtgttgacat tacccaaaag accgagaaat cctttgtagt aatataaata accgtgttca
     1561 aagaaagcgg tacatccctc ttttgcagaa gcaatcggtt taatattttt atgatcagtt
     1621 ccgtccatac tcatcctgca caaccaataa ctgttttgac aaacggaaat atagtagata
     1681 tatcggtcat agtatgcaat taagtgtgtg ttagcatacg cattacagtt ttcatcacgg
     1741 tcattgtgca gacaatcagg cttggaacac aatttcatat attttgaaat accgtcggaa
     1801 ctaaaatata taaacctgtt tttacaataa tagcaacctt tgtatgtttg aacaaattga
     1861 caatgagagc aataatccga ttggcggaaa cgtgtaaatt ttttgtcgtt aatatcgccg
     1921 gtaaaaacct tacctgtttt tgtgttgtta tcaaatccgc tatccgtatc ggaaacggtc
     1981 ttacaggaag gtaaaaatac cagcattact attaataaaa aacatataac ttttttcatt
     2041 tttaataact tattcttgca taattgtttt tatcaagtaa attatactac agtatattgg
     2101 caataatgta ataaataagt gatataatct atctaatatg tttgagagga gataaaccgt
     2161 tttatccatg atgaaacagt atgaataaat tatgcttata aagaaaaatc aaaaagtact
     2221 tatgattggt gattcggtga ccgattgcaa cagaagagat cagaattccg cttatctggg
     2281 acatgcttat ccgcatatag tggcatccaa gttttatctg gaacatccgg atatggatgt
     2341 tgaagttata aacaaaggca tatcgggaga tacatcaaaa atgctggtgg acagatggca
     2401 ggttgatgtt gtggatatga aacccgatat attgaccata cttataggga taaatgatgt
     2461 atggagacat tatgacacac ccgatgtgac aaggagagtg tccaaagaag actatataaa
     2521 gaacatgcgc tttatgatag aaagcatgat agacaagaca aaaaagatta ttattctttc
     2581 acctttctat cttgaagaga atatgtccaa gcccatgaaa gctcaggcac acgattatat
     2641 agttgcttta agagcacttg ttaaagaata tgatgtcctg ttcatcgatt tgcagaagga
     2701 atttgagaaa cttatgaaaa agtacacata tgaccaactt acttttaaaa ccgataagat
     2761 acatcccaca atgttcggtc aatatgtaat agctacactg atttatgata cgctgacaaa
     2821 atagcggtta ttcaaaaagc ggtgtggaaa gatatcgctc acctgtatcg ggaagaataa
     2881 gtgcaatgtt cttcccttta ttttcccctc ttttagcaat ttccacagct gcggccagtg
     2941 ccgctcccga tgatacgccg caaagaatgc cttctgtacg tgcaaggttt cctgtccact
     3001 cgtaagcact ttccgtctta acgggaaaaa tctcgtctac aagcttcata tctaaagttt
     3061 caggtataaa tcccgcacct ataccggaaa taccgtgtgc accgggctgc ttacccgcca
     3121 tggcttgcga ttcaaacggc tccaccgcta caatatgcac attcttttgt cttttgaaat
     3181 atcttgcaca gcctgttaag gtaccgcccg tacctaccgc ggctacaaat atgtccagtt
     3241 tgcctttcag ggcatcaaat atctctttag cggtggtctg ctcgtgaatc agaggattat
     3301 agggattctt aaactgttcc agcttaaaag catcggtata ttctaaagac ttatctatcg
     3361 cacctttcat gccataagcg gcatcggtaa gcaccacctc tgctccgtaa gctttcagca
     3421 gcaacaccct ctccttactg gcattgtccg gcatacagat aatgaggttg taacccattt
     3481 ttgcacaaat catggccagt gcaatgcccg tattgccgct ggtagcctct acaatgctta
     3541 tttttcgatt attcttaact gcatcgatat attctctatc tttttgcaaa ccccgtatta
     3601 acgaatatgc cgtcctgtcc ttgacacttc ctccgggatt aaagctctcc attttagcga
     3661 atatatttgc attaagttta tacttctcct ttattgcgca caactccacc aaaggcgtat
     3721 ttcctatggt atcaagaatc tccttaaata cagtcatcta taatccctcc tccgattact
     3781 tggtcgtata aataaaaaac ggcagattgc ccctttgcgg ccgctcttac aggttgatca
     3841 aatactacct ttattccgta atctgttttt tcaataacgg catcaacggc agccatggta
     3901 tttcttatct taactttaac tctcaaagga tcttctgctt tgtcgggttg tataaggtga
     3961 atatctttta ccgttaacac ttttgtgtaa agatcttcta caggacctaa agtgacggta
     4021 ttgctttttg cattcttact tatcacatac attctttgat tgaagccttt attaagacct
     4081 cttctctgac ctgtagtgaa atggatatga ttggctccct tacccaacac attaccttct
     4141 ttatctatga aagtacattc tttatattta cccggtcttt tttctttgat taaatcggta
     4201 tagtcaatat ctttgataaa acatatatcc tgactgtcct ttttatcctt aactgttaaa
     4261 cggattttag ccgcaatatc tcttacctgt tccttattca tatccgatat gggtaaaagc
     4321 agtcttgcca actgttgtct gctcagtctg taaagaaaat atgactgatc cttttctttg
     4381 ttttccgatg ttcttaaaat atagcggtcg gacactttat catattgtat gtttgcataa
     4441 tgaccggtag ccacatagtc aatgctcaga ctgtccgctt tgtctgtaag attcttaagc
     4501 ttaaaagaac tgttgcataa cacgcaggga ttgggtgttt ctccgcatat gtatgagtca
     4561 acaaaatatg aaactaccgt atcattgaat tctttctttt tatcttctat atataaagga
     4621 atatccagtg tttttgcaat agcctttgca tcttctgccg attttaactg attatccgac
     4681 aaaatatatg ttatgccata tactttatag cccttctctt taagcagata agcggaaacg
     4741 gcactgtcca caccgcctga aagaccgatc agtaccgaac ctttgtatcg gtcaattgcc
     4801 accggcacca cccgattgga atgtcgcatc gataaatacc aatccgtcgg atacggcaaa
     4861 ggatttcaga tctttcagta ccgatatatc cagagaaacg gtttttccga tgtattcgga
     4921 gaaaacacct cttacttcac tgcccaaatt gaaaagattg acaaagttca taacatgaac
     4981 ctctccggta tatatatcat ttatctttac cataagtttc ccgtcctgca ctccttcaac
     5041 ggttgtatga agcgtaaact ttgtctttgt tattaccgat aaggccatat cgggcagata
     5101 tgtcacccct tttaatgcat caccgtccag atatacatca atatgcaacg agttgtcggg
     5161 ttgtatgtta aatagcgcac tttccgcata aaaagcctta aggtcattct tggaaatatt
     5221 gaacatagcg gtaatttctt ccgaggtgaa acctacattc tccattttta tgggcttgtt
     5281 tatattcatt atcgcttctt cgggtgtgga gtaagcacct aactcgtcgc tgcttattcc
     5341 catcttttca tacatgctga aagtaccctt ttggacatcg ggcttttcta cgggttgttc
     5401 catttcatcc ataaaatgat ttaccgctct agcggtaaaa tagccgctga ctcccccgct
     5461 taaaataatg agtactgcta tgattataat ccaaactttg tttttcatcc gccacctcct
     5521 tgaaaatcaa actactctat ttcataaatc gctcttttgc atttccgaca cacagattat
     5581 atatcagttt ttttgcacat tcaatcgggt attcgtcaaa atctacccag ttacctatca
     5641 ctgagcataa tattcttctg taataatcat gacgtgcata agacagaaaa ctgcggctgt
     5701 cggtaagcat acccagaccc gcacctaagc aggacagtga agccacattc tttatattct
     5761 ccgctattcc ttcataatgg tccatatgcc accaggcagc tccgaataaa acatccctaa
     5821 aactgccgca cgcagtaaga gcctgatagt atgatgcggg atttaaaata tatacgacgg
     5881 tttttgccag ttgattacgc ttatccatct cgtccaataa ccgcgcaaaa gacaccgcat
     5941 ccaatgagtt ggaggaaatg tcacacccgc aatccggtcc gattgattca aaaagcctgg
     6001 tattggtatt tctgtatact cctgtatgta tctgctgagt tatatctctt tttttgtatt
     6061 ggcccgaaag gtatacgaac atatagccca agaatttttt ataatcaccc atttcaactt
     6121 ctttgccttt cagggcatgc ttaaatgccc tgtcggctgt tgcatagtca aaatccgcat
     6181 ccgcttcata ctgtattccc acatcggaaa gagagcatcc ctgttttgca aaatagtcca
     6241 gtcgcttttc tacggcttca cacagcgatt tgaggtcggt tatatttgtg cctgatactt
     6301 tttccagtct ctttatatac tccgcataat ccggcttgtt tataagaagc acattatcgg
     6361 ttctgaaagt cggcgcaact actgttttga aagacttgtc tgcttttata agtttatgat
     6421 attgcaaatc atctgcaata tcatcggtgg tcgctatgta tatgacattt gaagaagcta
     6481 tgagttttct cggacttaac ttcttttgct ttataacggc attggctttg tcccagatag
     6541 tttgtgcatt ctcttccgaa atagaggtat cggtatcaaa atattgcttt aattccattg
     6601 ctgaccaatg atataaaggg ttgccgggtg agtgctctat agctttacaa tatgcgaaaa
     6661 acttatcttt ccaaccggct tttcccgtta tatattgctc ttctataccg aaagctctca
     6721 tcagtctcca cttgtaatga tctttttcca gccacataac accgatattg tcaaaaggct
     6781 tatcctcata tatttccttg ggacttaaat ggcagtgata atccgctatg ggaaggtctc
     6841 ttgccacctc gtcgtataaa agttgtcccg cttttgagta tatatatttt gttttattca
     6901 tccgttgcct ccacatgtcc ctgaatcctc tgtataatca tatccagagc caccaaatta
     6961 tatccgcctt ccaaaacaat tatatcggca tttctctttg acggttccac aaactgctcg
     7021 tgcatgggtt ttacggtgga caaatattgg gtaaccaccg aatctaaact tctgcctctc
     7081 tcttttacat cgcgtaaaat ccttcttaat atcctgacat cgggatcggt atccacaaat
     7141 atccttatat ccagcatatt gagcaattcg gggttctgga atatgagtat accttccact
     7201 attataactt tggtgggatc tattttacgc gtctcctttg ttcttgtatg ctcggaaaag
     7261 gaatagacgg gacattcgat actgtgaccg ttttttaact gttttaagtg ttcaatgaga
     7321 aggtcggttt caaaggaatc ggggtgatcg taattggtct ttactctttc ctcaaaagat
     7381 atatcgtcct gagagcggta ataatcatca tgccttatca cgcttatatc cttaccgaat
     7441 cg
//
LOCUS       DAOSOM010000065         6825 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_11200_length_6825_cov_7.542688, whole genome shotgun sequence.
ACCESSION   DAOSOM010000065 DAOSOM010000000
VERSION     DAOSOM010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6825
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11200_length_6825_cov_7.542688"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..6631)
                     /locus_tag="PLF14_09570"
     CDS             complement(<1..6631)
                     /locus_tag="PLF14_09570"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOT71567.1"
                     /translation="MKHMARRFFALLMTLVMLLTMIPATVTANPGEPEVFVVAGRITD
                     SVTKKGLSGATVRLSNAEKEYTDTTDAFGYYQIDAREAGTYEISASLRGYTTEMKNDF
                     VLDGTKDWFAELIPDGNPCIDIYANIKCVYSGIALSDVTVKAIHSSEGPFIGQTDEHG
                     ALVFENLPEGTYTFTVNETGQPGWESYISGEYTVMQDQLLNCSLKPNYESLTVKTNGF
                     DAFSETANSPIRNVTVRLYGVDPKDNDVVLIKLPPQTTGENGTVTFENLVPIDWRIEA
                     YSPGYDSIPDIYSDDIIVKATEDGTLELDSVTLDMVFHPATLIANLKSVYDDDEIYTG
                     LEVNLCGINGTATEGLTRTVILFEGETFAEFTNLLPGQYDISTYGNAERKVDILVQEQ
                     NLYQYRVPNLEYGLYFPERVFSVTFQGQTPAVAREDQTVEIDLSLEVEKANYSGFLYK
                     IDMDKLGVLKTKPASNTVMTLVPSEYYPQNPEYGKTYTVTTDEFGHYSFNVYPGLYGV
                     TVTGMDYYWGAYADYRQRYIPIGEQEYYEYYGCYGWPYADTWQGDFASAIAYMGVGES
                     IDRTGYADIGGMPLSSGITEGDLFMNEKVCSIELDVDYQASRNPWRTTFTGKIDNTKN
                     PPDYKFCASWFNAANYGTEFSCNNIKVNAEQNSLPYKFVRVKPGTHDINITAREGETN
                     VPRYILYYGVGESEWVPWPGSTFEFYDFPAPGILPVNFPADYCDFERRDTLWPAGYNG
                     VDLEIRGVKEFKVSVLNQYIFHWVPPFKDSNGVITPGYYKSLAESFEESGGLYSKDDN
                     LYSINSGRNRIYGWSSEIFPNQLFGSPVFNTWGLTEDYKMMLPDVPVTLYLTVDPMND
                     DDVRGWAEIDFNPNTGRVTRVLYEDGPDNNTRKGLDKAPAILNPFTIKVRARQIYTGE
                     EINGISFKDTYTDIQYVSGQDYPNRVERPETYLHKIDVGNYNLLAIDMTSEYDTASAT
                     LTVMIDLIPNTEIELTVKDSKGALIPGAAVQLKSAKGFDEFLKSDTDGSVRKWDLKDE
                     NYTLVIDAPGYCTYTHELKAETLVASGKWKTFIHEAILVPLERPEISDESFDRFGGFL
                     PHAGRVTSGDNYELVCILSREENAAKDALTLTVEAALQQQKYDEVTDKVIGMYIIDGK
                     KFEVDDKSREGDYTEIDAATLPDPTSPQAVKEWLDKLSNRSAGLENVFFRYFDAECIT
                     QRGDNSDMADFSGEIMIYDLPAGPFKPIFIAVTEKGAYNIYTPVIAEDKQLVGLKKDG
                     FLTFFHNVTEMYLEKRPFVLPVFIVPFFEFCPALDEATNLPEFTAEISEDEDNPGYLV
                     YEYSLAVTADAGMANPGGSAGSPTSYLPDQLGIEFGGTFSASVSGKDKSINNSVALSV
                     GAEFETEKYLPAAISDVATMEGTATGTASVESGIEYESDAKPSATSSNIGLTGELATT
                     TNISGVGVAGKLFGGGALGTVFRTLKKWGIDLGVTIDANVGLNVGVGMTDNLKTPTAP
                     DKLDLDVGLGLGAKAGLYGSAFNGSAGARGTIGFGGEHEKFESSMMTFDVKFVYDVEK
                     GEFRADPATLKGVCEAEFEAFIDTAFFAARKSWNFEAFSVDMQFGTETKFTLTPVGVK
                     NHEINRDTFGTSEFLADETSGIMVKDYLALATYATDENSGNILYTDLVSQGGDMRLQL
                     SKFNGTTWQQPVTVISTDGAIGSCDLITLSDNRILITYSLIAKEDKYSLFPNSEIRYT
                     VGTVGANGWEESASGSVAQLSKSYAFKSYLTGSTNPRLVCALTGGTGATGFDIAGYKW
                     NGSGFDSAVMLKKNVKAFGINVAGNDNEAVVAYVEATGKLLALSWSGVNTVNDNVVAA
                     ETGSTSAVAVDANGFYIGCTSKGSIDFYTRGISPGSAWTKKGTPVSNVSAIEMDLAVN
                     GDKLVLSFIKPSEAYFDADNKLVVGNEGIASVTLAKDGTLYDEQRTYLENSQFYRNLS
                     IVCVNGLAYAFAEDLYEDTADLVFVKIYDADAPVNFFGTVTITGNERFNEQLTATVSE
                     DNSVSKNFTYTWKRGGAEIATGATYTTVAADIGKTLVCEVKTDDHQGVIRKATGVIAK
                     AAGPAAPAGLAGVAPTVAGGNDGKITGTDRTMEFSQTAAGVYTACRGVEITGLTAGTY
                     YVRIAGNNTTEAGAYRAVTVGAGAQQPDDPPIPDDPVIPDDPVIPDAGTGQDTSKTIP
                     TTDGKAQVDYAQSEEVVSLD"
BASE COUNT         1747 a         1663 c         1357 g         2058 t
ORIGIN      
        1 tatccagtga tacaacctct tcactctgag cataatccac ttgagcttta ccgtcggttg
       61 tgggaatggt tttcgatgta tcctgtcccg ttccggcatc cggaatgaca gggtcgtcag
      121 gaatgaccgg gtcatcggga atcggaggat catccggttg ctgtgctccg gctcctaccg
      181 taacagcacg atatgctccc gcttctgtgg tattgtttcc tgcaattctt acatagtatg
      241 ttcccgcagt aagtcccgtt atctcaactc ctctacaagc ggtatatact ccggctgcag
      301 tctgagaaaa ctccattgtt ctgtctgtac cggtaatctt tccgtcatta cctccggcaa
      361 cggtaggtgc tacgcctgca agtcctgcgg gtgccgcagg tcctgccgct ttggctatta
      421 cacctgttgc ctttcttatc acaccctgat ggtcatccgt tttaacttcg cagacaagtg
      481 tcttccctat gtcggcagct accgttgtat atgtggctcc tgtcgcaatt tctgctccgc
      541 cacgtttcca tgtgtaagtg aagttcttgc taacactgtt atcttcacta acagttgccg
      601 taagctgctc attaaatctt tcgtttcccg ttatggtaac tgtaccgaag aagtttacgg
      661 gtgcatcggc atcgtaaatc ttaacaaata ccaagtccgc cgtatcttca tacaaatctt
      721 cggcaaatgc ataagccaat ccgttgacac atactatact cagatttcta tagaactgac
      781 tgttttccaa atatgtcctc tgttcatcat acagagtccc atccttagcg agtgtaacac
      841 ttgcaatacc ttcgtttcct actaccagtt tgttatcggc atcaaagtat gcttcggaag
      901 gctttatgaa acttaaaaca agtttgtcac cgttaaccgc aaggtccatt tcaattgcac
      961 ttacattgga aacgggggta cctttctttg tccatgcaga gccgggacta atacctcttg
     1021 tgtagaagtc aatgctgccc tttgatgtac agcctatgta aaaaccgttt gcatcaactg
     1081 caacagccga tgttgaacct gtttcggcag caacgacatt atcgtttacc gtatttacac
     1141 ctgaccagct aagagcgagc agtttaccgg ttgcttcaac ataagcaact acggcttcgt
     1201 tgtcattgcc tgcaacattt ataccgaaag ctttaacatt tttctttagc atcactgcag
     1261 aatcaaaacc tgagccgttc catttgtacc ctgcaatatc aaagcctgtc gctcccgttc
     1321 ctcctgtaag agcacatacc aagcgaggat ttgtgcttcc tgtcaggtaa gatttaaaag
     1381 cataagattt ggacaattgt gctacactgc cggaagctga ctcttcccaa ccgtttgctc
     1441 ctacagttcc gactgtatag cgaatttccg agttggggaa caaactatac ttatcttctt
     1501 ttgcaattaa actatatgtt atcaatattc tgttatcgga cagagttatt aaatcgcaac
     1561 tgcctattgc accgtcggtt gaaattaccg ttacgggctg ttgccatgtg gtgccgttaa
     1621 atttactgag ctgtaacctc atatctccgc cttggcttac aaggtcggta tagaggatgt
     1681 taccgctgtt ttcgtctgtg gcataagttg caagggccag ataatccttt accattatgc
     1741 cggaagtttc atctgccaag aattcggacg ttccgaaggt atcacgattt atctcgtggt
     1801 tcttcacgcc tacaggggta agcgtaaact ttgtttccgt tccgaactgc atatctactg
     1861 aaaacgcctc gaaattccag ctttttcttg cggcaaaaaa ggctgtatca ataaatgctt
     1921 cgaattcggc ttcacatacg cctttcagcg ttgccgggtc ggcccggaat tctccctttt
     1981 caacatcata gacaaacttt acatcaaagg tcatcatgga gctttcaaat ttctcgtgtt
     2041 caccgccgaa acctatggtt cctctggcac cggcacttcc gttgaaagca ctgccgtaaa
     2101 gcccggcctt ggcaccgaga cctaaaccta catctaaatc taatttatcc ggagctgttg
     2161 gggttttaag gttatccgtc attccgacac caacgttcag acctacattt gcatctatgg
     2221 tgacacctaa atcaattccc cactttttaa gtgttctgaa cacggtaccc aaagcacctc
     2281 caccgaacaa tttacctgca acacccaccc cgctaatatt cgtagtggtc gctaattcgc
     2341 cggtaagccc gatattcgat gaagtagcac tcggtttggc atcactttcg tactcgattc
     2401 cggattcaac ggaagccgtt cccgttgcag ttccctccat ggtggcaaca tcgcttattg
     2461 ccgcaggaag atatttctct gtttcaaatt ccgcacccac ggagagtgct accgaattat
     2521 ttatactttt atccttaccc gaaacactcg cactgaatgt gccgccgaat tctataccca
     2581 attggtcggg cagataggat gtgggagaac ccgccgaacc gcccggattt gccatacccg
     2641 catctgcggt tacagccaga ctgtattcat atacaagata gccggggttg tcttcatcct
     2701 cgcttatctc ggcggtaaat tccggtaaat tggtcgcttc gtcaagtgca ggacagaact
     2761 caaagaacgg aactataaat accggaagta caaacggcct tttttccaga tacatctctg
     2821 taacattatg gaagaaagta aggaaaccgt cttttttcag acctacaagc tgcttatctt
     2881 cagcaatgac aggcgtataa atattgtagg cgcctttttc ggttaccgct ataaatatcg
     2941 gtttaaacgg acctgcgggc aggtcatata tcattatctc gcccgagaag tctgccatgt
     3001 ctgagttgtc gcctctttgg gttatgcact cagcatcgaa atacctgaaa aatacatttt
     3061 ccagccctgc ggagcgattg ctaagcttgt ccagccattc ttttaccgct tgcggagatg
     3121 taggatcggg aagtgttgca gcatctattt ctgtatagtc accctctctg gacttgtcat
     3181 ctacttcaaa cttcttgccg tcgattatat acatgccgat gactttgtct gtaacctcgt
     3241 catatttctg ctgttgcaaa gccgcctcaa cagtcagtgt cagagcatcc tttgcggcat
     3301 tttcttcacg acttaatatg cacaccagtt cataattatc accgcttgtt acacggcctg
     3361 catggggcag gaagcctccg aaacggtcaa agctttcgtc gctgatttca ggtcgctcca
     3421 aaggaactaa gattgcttca tgtatgaaag ttttccattt tccggatgct accaatgttt
     3481 cggcctttaa ctcatgagtg tatgtgcaat agccgggagc atctataacc aaagtatagt
     3541 tttcgtcttt aaggtcccat tttctgacac tgccatctgt atctgatttc agaaactcat
     3601 caaaaccttt agcgctcttc aactgaaccg cagcacccgg aatcagagca cccttgctat
     3661 ctttaacagt cagctctatt tcggtattgg gaatcaaatc tatcatgact gtaagagttg
     3721 cgcttgcagt atcatactct gaggtcatat ctatcgcaag cagattatag tttccgacat
     3781 ctattttgtg aagataggtt tccggccttt ctaccctgtt gggataatcc tgtcctgaaa
     3841 catattgtat atctgtgtaa gtgtctttga aagatatacc gtttatttcc tcgccggtat
     3901 agatttgtct tgcccttact tttattgtaa atggatttaa tattgccgga gccttatcta
     3961 atcctttgcg tgtattgttg tccggtccgt cttcatataa aactcttgtt acccgacccg
     4021 tattcggatt aaaatctatc tctgcccatc ccctaacgtc atcatcattc ataggatcca
     4081 cagtaagata tagggtgaca ggcacatccg gcaacatcat cttataatct tcagtaagac
     4141 cccaagtatt aaatacggga gaaccgaaca gttgattggg aaatatttcg gaactccatc
     4201 cgtatattct gttacgacca ctattaatac tgtataaatt atcatctttc gaatataatc
     4261 cgccggattc ttcaaatgac tccgctaagc tcttatagta accgggtgta attacaccat
     4321 tactgtcttt gaaaggcggt acccaatgaa aaatatactg attgagaaca cttactttga
     4381 attccttaac tcccctgatt tcgagatcta ctccgttata tccggcaggc caaagagtat
     4441 cgcgtctttc gaagtcacag taatcggccg ggaagttgac aggaagtatg cccggtgcgg
     4501 gaaaatcata gaactcgaaa gttgaaccgg gccagggtac ccattcactt tcccctacac
     4561 cgtaatataa tatgtatctc ggtacatttg tttctccttc ccttgcagtg atattgatat
     4621 cgtgagttcc cggtttcact cttacaaatt tgtatgggag agaattctgt tcagcattga
     4681 ctttaatatt gttgcaggaa aactcggttc cgtaatttgc agcattgaac catgaggcac
     4741 aaaatttata atcgggcgga ttttttgtat tatctatctt gccggtaaaa gttgtgcgcc
     4801 agggatttct gctggcctga taatcaacat ctaattctat cgagcagact ttttcgttca
     4861 taaacaggtc tccctcggtt attcccgaag agagcggcat gccgcctatg tcggcatatc
     4921 ctgttctatc aatagactca cctacaccca tataggctat agcggaagca aagtcacctt
     4981 gccatgtatc tgcataaggc caaccgtaac atccgtaata ttcgtagtac tcttgttctc
     5041 caatcggtat gtaccgctgc cggtaatccg cataagcgcc ccagtaataa tccataccgg
     5101 ttacagttac accgtagaga ccggggtata cattaaaaga ataatgacca aattcatcgg
     5161 tagttactgt ataagtctta ccatattcgg gattttgggg ataatattcc gaaggaacca
     5221 atgtcattac cgtattggag gcaggctttg ttttgagaac acccaacttg tccatatcga
     5281 ttttatagag aaaacccgaa tagtttgctt tttcgacttc taaagacaaa tcaatttcaa
     5341 cagtttgatc ctctctcgca acagcgggag tctgaccttg gaaagttacc gaaaataccc
     5401 tttcaggaaa atacaagcca tactccagat tgggaaccct atactgatag agattttgct
     5461 cttgcactaa tatatctact ttacgctcgg cattaccgta tgtggaaata tcgtactgcc
     5521 ccggcaaaag atttgtaaat tccgcaaacg tctcgccctc gaataagata acggtgcgtg
     5581 ttaagccttc ggttgcggta ccgtttatac cgcaaagatt gacctcaagg cctgtatata
     5641 tttcgtcgtc atcatagaca gatttgagat tggctatcaa ggttgcagga tgaaaaacca
     5701 tatccaaagt aacgctatcc aactcaagag ttccgtcttc tgtagcttta actataatat
     5761 catcggaata aatatccggt atggagtcat aaccgggaga atatgcttca attctccagt
     5821 ctatcggcac aaggttttca aatgtcaccg ttccgttttc gcctgtagtc tgcggtggaa
     5881 gtttaatcag taccacatcg ttatctttcg ggtccactcc gtaaagtcta accgtaacat
     5941 tcctgatagg actgttggct gtttcggaga aagcgtcaaa accgttggtc ttaacggtca
     6001 ggctttcgta attaggcttt aaagaacagt ttagcaactg gtcctgcatt actgtatact
     6061 cacctgatat atagctttcc catcccggtt gaccggtttc atttaccgta aaggtgtatg
     6121 ttccttcagg taggttctca aaaaccaagg ctccatgctc atcggtctgt ccgataaagg
     6181 ggccttcaga gctgtgtatg gccttaaccg tcacatcgga cagggctatg cctgaatata
     6241 cgcacttgat attggcataa atatctatgc agggatttcc gtcaggtatc agttcggcaa
     6301 accagtcttt tgtgccgtcc agcacaaaat cgtttttcat ttccgtagta tagcctctaa
     6361 gcgaagcgga tatttcataa gttcccgctt cacgggcatc tatctgataa tacccgaaag
     6421 catcggtagt atcggtatat tccttctccg catttgataa tcttaccgtc gctccgctta
     6481 agcctttctt tgtaacacta tcggttattc ttcccgctac aacaaaaact tccggctcac
     6541 ccggattagc agttaccgtt gcgggtatca tggtaagtaa cattaccaat gtcatcagta
     6601 gtgcaaaaaa tcttcttgcc atgtgtttca ttttcttcct cctcgtaccg acaaatatta
     6661 aatcaattaa aaaatcgttg agatctgact cgagtatatc ggttgttttt tctacattca
     6721 acagtttggt cgcattcggt tatttcccgt cataaacggt caaaaattgt acaaagcaaa
     6781 ggtaccacac ctttggcggc ggcatggtac ccttgttgtt gagga
//
LOCUS       DAOSOM010000066         6728 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_11422_length_6728_cov_4.850592, whole genome shotgun sequence.
ACCESSION   DAOSOM010000066 DAOSOM010000000
VERSION     DAOSOM010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6728
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11422_length_6728_cov_4.850592"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2601
                     /locus_tag="PLF14_09575"
     CDS             <1..2601
                     /locus_tag="PLF14_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT71568.1"
                     /translation="KESRGKHDINENCYIDFDGKCVVENGEQKKFSRRHMDTLSLLVQ
                     NLNHTVSINDIHMKLFGYEYDPRRCDNNLVQIQRLISQLRNNSILSRCIETDDKGYRL
                     TDSFPLPSLKPSYSHKNDNTITPFYNCPFYENRKNMIQKLHKEAFVRCPVHAISGERG
                     MGKTELAKEYANSCITDGYKNIIFTTYSDSGLKDTIISLPCNALNKRDNRYKVIISLL
                     KGLEKPSLLIIDNIDYSNNQDLSELSDKSECYMDLINTGCHILMTSKTNLSNCYAIEQ
                     TEIKPLEMDILVQLFFKIMFDIKEKDSDIAQDIIAKKIAEYLMQKHDREYHDFIESIT
                     DLIDNRLMRNTYLVTLVAKLSQIKDPYDIIVALASDNTKSLDDTVSVFKDGEHKKDNI
                     INLYYSLFKISGITDTDIDLQLLYNLALLPISGFNYDTFFDNSFDMENREQLKAAFDK
                     LLNGYYAFNHYGTISIHPFIREIILTKIDRFDFNYIEKYVSYITDIIDNATYDSDLTN
                     NLTLATSLYSGLKKIGQIPLSYAKLTACIANVYRIITNNQLGYKYGKHAIELLDSLDM
                     DALSEDDKLEKASCLNQAGYAVLQYTSAKDRLSIAYKAISCAEQITESIKNKLNQPES
                     ISNKCDRLLTVINGNLGAYHYASRDYIKALDYHKKTKVYREALVQSEPNSVNLNLLAT
                     AYRNIGSDYFHISRSCMDEEKYNYLKLSYENQLISASIFEKVNTAQHIETIISNNRLI
                     GAGIMLTNLYFDLCEQKKDVTPPFESIKDSLAFFSDKIKAGIIYMHDVGVFTKEVEDS
                     IGKTRDLLSILENRNIVDKSILTNITETIDVAASIPGIDMSFISICYDMIDKVTEMSM
                     IKIRETSSNE"
     gene            2824..3597
                     /locus_tag="PLF14_09580"
     CDS             2824..3597
                     /locus_tag="PLF14_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71569.1"
                     /translation="MTNEGEAARWLLKNDEIVEKYSKLVLEASVKLGLIGITDTVLNS
                     PDYILPLGGALISNFKRCELAKTLIDTHNFESKVIALSTMRPLSEQEITFYSNTPAKD
                     AVFEFEAIASGLSTTFDIRSYTEETERKDNYNLNWTIRKYSEKYGNNDIYAISAPSSE
                     PDIRRANSVDCFDFFFKRLNVLPHSNIINCTSQIYCPYQQVRALSFAIKHTVNFDTVG
                     YIPNQDSASTPKLLNQPVNYLQEIKATIDAIYDFVNRFS"
     gene            3806..5020
                     /locus_tag="PLF14_09585"
     CDS             3806..5020
                     /locus_tag="PLF14_09585"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOT71570.1"
                     /translation="MKTIIKQTICTLIVLVLCLSYLNVDAAIMPRWNSNKVYSKVTAQ
                     ISPAVKCSTDKQRYQPSDKVVVKWQADRKAAYYTIELLNLTKKICVNMNKKIYAPKCT
                     YEITDLTPGCNYSISVTAHNSDGTRRQKGNTVKFTVAPISIKSIEIKGPSTIIVGQKT
                     TLTAVVKSNSPYKAPVKWRIAKSNGNNVTIDTSTGVIIVKPSAKMGDSIVVEACAEGY
                     KATRVIKVQKAYILPNHTYFSQINYPQLKRTGCYITSSCMILSNLGFETNPNETYKAN
                     GNTTYLYRGRVFDYYNNKYSKQLGFKVVVKKNNSEKVEGKSDDDKILRIIQYLNEKDR
                     DHGGVFVQFKNGTRTHAMVAIGYRINSKGQYEILYDDPGRVKNSCVPIEKTSFRTKDI
                     IYLTVYSKAPVR"
     gene            5390..6619
                     /locus_tag="PLF14_09590"
     CDS             5390..6619
                     /locus_tag="PLF14_09590"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990814 - DNA/DNA annealing activity
                     [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOT71571.1"
                     /translation="MAEKLLFAKEAAEYLNITTQRLNILVKEGKVKPLKKNLSGTVFH
                     IDELDRRKEELKIFDKISPGGKKGMFQFDTTEKREALNFSTLINALEITEQRLDPIFT
                     DFSKETDVSVFMTHDESLLGKYSGYFKVGVERLKKEFDIACRAFSNLKPTDEIIKRGA
                     HDYSPLLAETQQAPRFLYLRGNKSLLFEERTVALVGSRRATESAKENTRLLTIALGNN
                     GITIISGLARGIDVTAHKTALEYGFNTIAVIGTNLNQYYPIDNKEVQMEIEKKGLVIS
                     QFSPAIKTQKWFFPLRNGVMSGLSLATVIMEAGETSGALKQADFALKQNRKVLIPKTA
                     LELKTITWPARYVERGAEVVENPKNVLEKLAESKIFKLENEETCKQLSFDVLSDENAK
                     SKTVKEKWTTQVVNLVL"
BASE COUNT         2496 a         1110 c         1201 g         1921 t
ORIGIN      
        1 aaagaatcta gaggaaaaca tgatataaat gagaattgtt atattgattt tgacggcaaa
       61 tgtgttgtcg agaacggaga acaaaaaaag ttcagtcgta gacatatgga tacattgtcc
      121 cttcttgttc aaaatctaaa tcatactgta agtataaatg atatacatat gaaattattc
      181 ggatatgaat atgatcctcg tagatgtgat aacaatttag tacaaataca aagattgatt
      241 tctcaactta ggaacaatag tatcctttca agatgtattg aaacagatga caaaggatac
      301 cgactcaccg attcttttcc tcttccttcg cttaagcctt catacagtca taaaaacgat
      361 aatactataa ctcctttcta taactgccct ttctatgaaa acagaaaaaa catgatacag
      421 aagttgcata aagaagcttt tgtacgttgt cctgtccatg ccatatccgg cgagagaggt
      481 atgggaaaaa cggaattggc aaaagaatat gcaaactcat gtataacaga cggttataaa
      541 aacataatat tcacaactta ttcagatagc ggtttaaagg atacaataat ttctctaccg
      601 tgtaatgcac tgaacaaaag agataaccgt tataaggtta tcatatcctt gcttaaagga
      661 ttagaaaaac cttccctgtt aattatagac aacattgatt attcaaataa tcaggattta
      721 tccgagttgt ccgacaaatc cgagtgttat atggatttaa tcaatacagg atgtcacatt
      781 ttaatgactt ctaaaactaa tttgtcgaat tgttatgcaa tagagcaaac ggaaataaag
      841 ccgcttgaga tggatattct agtacagtta ttctttaaaa tcatgtttga tataaaagaa
      901 aaggattccg atatagcaca ggatatcatt gcaaaaaaaa ttgcagagta tcttatgcaa
      961 aagcacgata gagagtacca tgattttatt gaatcaatca ccgacttaat agataaccga
     1021 ttgatgagaa acacatattt agttactctt gttgcaaagt tatctcagat aaaagatccg
     1081 tatgatatca ttgtagccct tgcatcagat aatacaaaaa gtctcgacga cacagtttcc
     1141 gtatttaaag atggtgagca caaaaaagat aatattatta acctttatta cagcttattc
     1201 aaaatttccg gaatcaccga tacggatatt gatctgcagc tattgtataa tttagcctta
     1261 ttaccgataa gcggatttaa ctatgatact ttctttgata attcttttga tatggaaaac
     1321 agagagcaac taaaagcggc atttgataaa ctgcttaacg gttattatgc atttaatcat
     1381 tacggaacta tatccattca tccgtttatc agagagataa tattaaccaa aatagataga
     1441 tttgacttca actatataga aaaatatgtc tcttacatta ccgacattat tgataatgcc
     1501 acctatgact ccgatttaac gaataattta acccttgcca cctcattgta ttcgggatta
     1561 aaaaaaatcg gacaaatacc attatcttat gcaaaactga cagcatgtat agccaatgtg
     1621 tatcgtatta ttacaaataa tcagctaggg tataaatacg gaaaacatgc tatcgagttg
     1681 cttgattcgc ttgatatgga tgctctaagt gaagatgata agttggaaaa ggcaagttgc
     1741 ttgaatcaag ccggttatgc ggtcctacag tatacaagtg caaaagacag actttccatt
     1801 gcatacaaag caataagctg tgcggaacaa ataacagaaa gtataaaaaa caaattaaac
     1861 cagccggaga gtataagtaa taaatgtgat agattactta cagtgataaa tggcaattta
     1921 ggagcatacc attatgcaag cagggattat attaaagctt tagactatca taaaaaaacc
     1981 aaagtataca gggaagcatt ggtacaatct gaacctaaca gtgtaaattt gaatctgctg
     2041 gcaactgcct atagaaacat cggatccgac tattttcata tatccaggtc ctgtatggat
     2101 gaggaaaaat acaactatct taagctgtca tatgaaaacc agctgatatc cgcatcaatc
     2161 tttgaaaaag taaataccgc tcaacatatt gaaacaataa tatccaataa cagacttata
     2221 ggtgcaggta ttatgttaac aaatttatac tttgaccttt gcgagcaaaa aaaggatgtc
     2281 actcctcctt ttgaaagcat aaaggattct cttgcgtttt tttccgataa aattaaagca
     2341 ggtattattt acatgcatga tgtaggagtt tttacaaaag aagttgaaga cagtatagga
     2401 aaaacaagag atttactttc cattttagaa aacagaaata ttgtagataa gagtatattg
     2461 accaatatta ctgaaactat tgatgttgca gcatccattc ccggtattga tatgtcgttt
     2521 atttccattt gttatgatat gattgataaa gtgactgaaa tgagtatgat taaaataagg
     2581 gaaacaagta gtaatgaata atcgtatcct ttgcccacct tcggatatat ctgaaactga
     2641 acgaataaaa actattattc accacgaatt ttataactgg ttaatgacac ctgagttagt
     2701 tgagttgatt aagatttata ataacaacga gaatccgccg cttctttacc gtattatgat
     2761 tacttaaaat ggctgaaaag ttttgtagct gtatgggatt ttcgcaaaaa acaatccgaa
     2821 gcgatgacca atgagggtga agccgcaagg tggttgttga agaatgacga gatagtggaa
     2881 aaatacagta aattagtact tgaagcatcg gtaaaattag gacttatcgg aattactgac
     2941 acagtactaa attctcccga ctacattctt cctctcggtg gagctctaat atccaacttt
     3001 aaaagatgcg aattagcaaa aacccttata gacactcata actttgaatc aaaagttatt
     3061 gcattatcca caatgagacc tttatcggaa caggagatta ctttttatag caacacacct
     3121 gccaaagatg ccgtttttga atttgaagca atagcatcgg gtttatcaac tacatttgac
     3181 attcgttcat atacggaaga aacggaaagg aaagataact ataacttaaa ttggacaata
     3241 agaaagtact cagaaaaata tggaaacaat gatatttatg caatttccgc tccttcttcg
     3301 gaaccggata taagaagagc caacagtgta gattgctttg actttttttt caaacgatta
     3361 aatgtcctac cgcattcaaa tatcattaat tgcacatcac agatttattg tccatatcag
     3421 caagttcgag ctttgtcatt tgcaataaaa cacacagtca attttgacac agtcggatat
     3481 attccaaatc aggattctgc aagtaccccg aagttactga accaaccggt taattattta
     3541 caggaaatta aggctactat tgatgcaata tatgactttg ttaaccgatt ttcgtagatt
     3601 ttataataaa aaacgctatt tcacatccat ataatcaaac tctcttttag atgtcgtttc
     3661 tttgatctga ccaataattg atgagttgat tttgttagga ttttgttagg aagctgacaa
     3721 actatttatg ctatcctaaa tgtgaagata cggagcaaca gttcttcaag acaaaatatt
     3781 tttaaaatta caaggaggaa agaaaatgaa aacgattatt aaacaaacaa tctgcacact
     3841 gatagttctt gtattatgct tatcctacct gaatgtagat gcagcaatta tgccccgttg
     3901 gaactctaat aaggtctatt ccaaagtaac ggcacaaata tcccctgcag taaaatgttc
     3961 taccgataag caaagatatc agccttcgga taaagtagtg gtaaaatggc aagcagacag
     4021 gaaagctgct tactacacaa ttgaactgtt aaatcttaca aaaaaaatat gtgttaatat
     4081 gaataaaaaa atatatgcac cgaaatgcac atatgagatt acggatttga ctcccggatg
     4141 caactattca atcagtgtaa cagcacataa ttccgacggt actcgcagac agaaaggaaa
     4201 caccgttaag tttacggtcg ctcccatttc aatcaaaagt attgaaataa aaggaccaag
     4261 cacaattatt gtaggtcaaa aaactacatt aaccgcagtt gttaaatcca actcgcctta
     4321 caaagcacct gtcaaatgga gaattgcaaa aagtaacggt aataatgtca ctatagacac
     4381 aagcacagga gtgattatag taaaaccctc tgcaaaaatg ggagactcca ttgttgtgga
     4441 ggcttgtgcg gaaggctaca aggcaacacg tgtgataaaa gttcaaaaag catatatcct
     4501 acccaatcac acatatttca gccaaatcaa ttatccccaa ttaaaacgta ccggatgcta
     4561 cataacaagt agttgtatga ttctgtccaa cttaggtttt gagactaatc cgaatgaaac
     4621 ctataaagca aatggaaata ccacatatct atacagagga cgtgtttttg attactataa
     4681 taataagtac tcaaagcagt tgggatttaa agtagtagtt aagaaaaaca actcggaaaa
     4741 agtagaagga aagtccgatg atgacaaaat cctaagaatc atccaatatt tgaatgaaaa
     4801 agacagagat catggcggtg tttttgttca gtttaagaat ggaacacgaa cgcacgcaat
     4861 ggtagcaata ggataccgaa ttaattcaaa aggacaatat gaaatattgt atgatgatcc
     4921 gggtcgggtt aaaaacagct gtgtaccgat tgagaaaaca agtttcagaa caaaagacat
     4981 tatctattta actgtttatt ccaaagcacc ggtaagataa gcgaataaac ctataacgat
     5041 agtatcagta aaactgtaat actataccaa tttcatatat cccgacaaaa cttttcatac
     5101 tcgggcaagt agtaaaaaag catagcggcc gctatgcttt ttacaataca aaatatatca
     5161 gccatttcat cttctgaacc tgagggagga aaaatcctca ctcttaaaac ctaccgctcg
     5221 caaggccttc tccacttcca atcgatgatc tacatacaaa gtatatttta aatattatca
     5281 catattctat caaacggtat attatagcat tgattgattc ttaagggttg acaaacttaa
     5341 acctcataat gtaatatatg gctaaagggg tgaatgaggt atagaaaaat tggcggaaaa
     5401 gttgctcttt gcaaaagaag cagctgaata tcttaatata actacacaaa ggttaaatat
     5461 attggtaaaa gaaggaaagg tcaaaccgct gaaaaaaaat ctttccggaa cagtttttca
     5521 tattgatgaa ttagatagaa gaaaagaaga attaaaaata tttgataaaa tcagtccggg
     5581 aggtaaaaaa ggaatgtttc aatttgacac gacagagaaa agggaggcat taaacttttc
     5641 aaccttaata aatgctttag aaataacgga gcagagattg gaccctattt tcacagactt
     5701 cagtaaagag acagatgtat ccgtatttat gacacatgat gaatctcttt taggaaaata
     5761 ttccggatat tttaaagtag gggtggagcg tttaaaaaag gaatttgata tagcatgtag
     5821 agctttttcc aacttaaaac cgacagatga gattataaaa agaggtgctc atgattattc
     5881 accgctttta gctgaaactc aacaagctcc caggtttttg tatctaagag gaaacaagtc
     5941 tctgctattt gaagagagaa cagtagcact tgtgggctca aggcgcgcaa cagaaagtgc
     6001 aaaagagaat acacgccttt taacaatagc attaggtaat aacggaatta ctattatatc
     6061 aggcttagca agagggattg atgtaacagc ccataaaact gctttggaat acggatttaa
     6121 tacaattgcc gtaataggta caaacttaaa tcagtattat ccgattgata ataaggaagt
     6181 acaaatggag atagaaaaaa aaggtcttgt aatctctcaa ttctctcctg caattaagac
     6241 tcaaaaatgg ttcttcccgt tgagaaacgg agttatgagc ggtttatctc ttgcgacagt
     6301 tataatggaa gccggcgaaa cctcaggagc tttaaaacaa gcagattttg ctcttaaaca
     6361 aaacaggaag gttctcattc ccaagaccgc tttggaattg aagactataa cttggcccgc
     6421 taggtatgta gaaagaggtg ctgaggtagt tgaaaacccc aaaaatgtac tggaaaaact
     6481 tgcagaaagt aaaattttca agttggagaa tgaagaaact tgtaagcaac tgagttttga
     6541 tgtcttatcg gacgaaaatg cgaaaagtaa aactgtaaag gaaaagtgga ctacccaagt
     6601 agtcaatttg gtgttgtaat gtttagatgc actaatgaat taaaaggttt ggctatagac
     6661 acagacagtt tttcagatat tccaatcgga gagtggaata gaagtagcac aattatccat
     6721 ttataaaa
//
LOCUS       DAOSOM010000067         6715 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_11441_length_6715_cov_4.834535, whole genome shotgun sequence.
ACCESSION   DAOSOM010000067 DAOSOM010000000
VERSION     DAOSOM010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6715
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11441_length_6715_cov_4.834535"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="PLF14_09595"
     CDS             complement(<1..1010)
                     /locus_tag="PLF14_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71572.1"
                     /translation="MLSNEAKKRYQAFWDRTSYERCTMYIFRKIKEDVVMQGASLEDK
                     WINLEKRTQEAQELYVNYKYYAEGFPTYNTNLGPGSMAACIGGDFELAPRTIWFDRNP
                     IIKDWSEVPELKIYEDSEMWQIITGLTEMLCKASQGRYYTSMTDIGGPLDIIASLRGT
                     EQLLYDLIDYPDDVKKVNQRIEELWKIMYNKLYAITSRYQNGMTSWMPIYCEKRYYPL
                     QCDFCAMISPDMFLEFVRPNLAAQTEWLDHSIYHLDGMDALRHLDYILDIPTLDAIQW
                     SAGAGKPALYDEYFFDLYNKISRSGKGLMLYYIPITELENVLKNISTKGLFITMLVEE
                     EK"
     gene            complement(1025..2317)
                     /locus_tag="PLF14_09600"
     CDS             complement(1025..2317)
                     /locus_tag="PLF14_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOT71573.1"
                     /translation="MKYNLLHPADQIVMLMNRIYRYGMTTTSGGNLSVLDENGDIWIT
                     PSGIDKGSLKRIDIMQVKPDGTIIGPHRPSVELPFHKHVYRTRPEAKAVIHAHPPASV
                     AFSIVRQIPNVHLVPNINRICGEVGMAEYDVPGSEALGENIAAAFAKGYDTVMMENHG
                     VIVIGKDLFDAFMKFETIDMCAKLEIRAKCIGNIKPLSQKHIDISLQKKGLPMQEFVP
                     SHYSSEERAVRREMCELIHRSYDQQLFTSTQGTFSVKLSDDAFVITPYGVDRKYITVD
                     DLVRIENGKYREAGKNPSRSVALHLKIYAMQPHVKSIIISHPPNMMAFAVTDCVFDAH
                     TIPESYIALRNVPKMPYGSSFLQPEMFARIFDKRTPVVLVKNDCVICTGSTLLQAFDR
                     LEVAEFSAKALIDAGVLGEMVRINEEQTAEIERFFKLD"
     gene            complement(2322..3863)
                     /locus_tag="PLF14_09605"
     CDS             complement(2322..3863)
                     /locus_tag="PLF14_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71574.1"
                     /translation="MSGIMLNWDDSAFFFGIHKGYGKSVKDKQEAIAYTEAVMGQYQG
                     TNVTDIVMCVNARLASYPSVTIENYGDSYRRKVQNGEQVDFKKTWVSAYDKIFNQWGI
                     DHFQIWIDKLKKMNIKPWISIRMNDAHETMKKTSCIAPDFYYDNPQYRRVTYSPVQLY
                     YDGLYDYGQAAIRKRFLDFIDETLGRYDVPGLELDWMREAYLFRYGMEQEGITILTGF
                     MKDVKKIVKKYEQKRGHGISVSVRVPSGVETCYYLGLDVAGWAEHGLINNIVITPRWS
                     TTETDMPVEVWKKLLKPYNVTVAAGIEVLMRQNREVPYTYNCKQTVYANSAAYFSAGA
                     DKFYLFNYMTMPDCFGTDPKDTALYNLHKDIKDILKACNSLENSISLDRRHLVTFKDF
                     TAPWEKSAYYVPALCNPDSNEPVIFRIRTGKTDKNSAAYIQMGIDCDDVLNDDDLLIY
                     LNSRQVKGLKKTEQPDYYIKDGRYICKIPDIDMINDINILQIWSRTKTFTITHIEIMI
                     KGKDI"
     gene            4115..4858
                     /locus_tag="PLF14_09610"
     CDS             4115..4858
                     /locus_tag="PLF14_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004602065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HOT71575.1"
                     /translation="MEKKGLNANHLKLIAIIAMTVDHIADLFYPGFPVKPLPIILHLI
                     GRLTAPIMWFFVCEGFYYTRNVKKYMLRMFIFAVISHFAYCFSFGIDPVPFRSGIFNQ
                     TSVMYPLFISVVVLWLEHEEKRMNKYLKHIIIFLLICSAFPADWSCIAVLAIVSMYKK
                     RGDLKGQMKMMLLWVFFYGLISFFFVSKVYALELIGVLMVYPVLKMYNGQKGKAGWMK
                     MFFYIYYPLHLVVIGIIRILMYGDVPLLF"
     gene            5000..5803
                     /locus_tag="PLF14_09615"
     CDS             5000..5803
                     /locus_tag="PLF14_09615"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HOT71576.1"
                     /translation="MKYRLIATDIDGTLLDDFGMLPENTYAAAAYLEEKGIKLVLCTG
                     RPLLGVLKIYDALKLTSPVVTNNGAMVYLGKDGPLIYSETMSIEDGLNVIKLNDILQC
                     TVCMWHNGVLYVSELNKYAYDYAAITGITPLLLNTIDLRKCKNGPTKFVFIDTLEKIT
                     AIKEMAARLAPSTVTAVTTRPYFLEFFSSSISKATALSKLSEALNIGREQMIAAGDGF
                     NDIDMLDYAGFAVVAGNADEGVKEHADMIACSNNDGVIAYIVDNLIKKS"
     gene            complement(5790..>6715)
                     /locus_tag="PLF14_09620"
     CDS             complement(5790..>6715)
                     /locus_tag="PLF14_09620"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOT71577.1"
                     /translation="ANKITAFEDKPLTYQAWDINIYYNEKPYEINDVTYSALVEDGPV
                     RKCIRVDRKILETTITQYISLECNSRKIDFKTVIDCNHDDLLLKAAFPVDVHTDYATY
                     DIQFGNVRRPTHWNTSWDYARFEVCAHKWADLSEDDYGVSILNDCKYGHDIKDSVIRL
                     TLLKSATEPDPDADRGLHEFTYSLYPHMGDFKKAHTVDMAYKLNFRPYAKYVEKGTGD
                     IENYRSFMTCNHENVIVDTLKKAEEGDGLILRVYECFNRRTECTIDLNFEVSKITECD
                     LMENDIADAVIEDNVLKFTIRPYEIKTFKIS"
BASE COUNT         1841 a         1465 c         1256 g         2153 t
ORIGIN      
        1 tctttttctt cctccaccag catggttata aacaaacctt tggtcgatat gttctttagg
       61 acattttcca attccgttat cggaatgtaa taaagcataa gccctttccc gctccttgat
      121 atcttgttat acaagtcaaa gaagtattca tcatacaagg cgggcttgcc ggctcccgca
      181 ctccattgaa tagcatcaag agtaggtata tccagaatat aatccagatg acgaagggca
      241 tccatgccgt caagatgata tatggaatga tccagccatt cggtctgagc agctaaattg
      301 ggtcttacaa attctaaaaa catgtccggc gaaatcatag cacaaaaatc acactgtaaa
      361 ggataatatc tcttttcaca atatatcggc atccaacttg tcattccgtt ttgataacgg
      421 gatgtaatgg catacagttt attgtacatt atcttccata actcttcgat tctttggtta
      481 actttcttaa cgtcgtcggg atagtctatt aaatcgtata aaagctgctc ggtacctctt
      541 aaagaggcta taatgtccaa cggacccccg atatccgtca ttgatgtata gtatctgcct
      601 tgcgaggctt tgcataacat ttcagtaagc ccggttatga tctgccacat ttcactgtct
      661 tcgtatattt tcaattcggg aacttccgac cagtctttaa tgataggatt gcggtcaaac
      721 catatagttc tcggagccag ttcgaagtct ccgcctatac atgccgccat actgcccggt
      781 cctaggttgg tattataggt gggaaaacct tctgcataat acttatagtt tacatataat
      841 tcctgcgctt cctgtgtgcg cttttctaaa ttgatccatt tatcttctaa tgaagcgcct
      901 tgcattacaa cgtcttcctt tatttttcgg aagatataca ttgtacacct ttcgtatgaa
      961 gtcctgtccc aaaaggcttg gtatcttttc tttgcttcat tacttaacat aaaacattcc
     1021 tcttttagtc aagtttgaaa aatctttcta tctcagcagt ctgctcttca tttatcctga
     1081 ccatttctcc caaaacaccg gcatcaataa gcgcttttgc actgaactcg gcaacctcca
     1141 gtcggtcaaa tgcctgtaag agagttgaac cggtacagat aacgcagtca tttttcacta
     1201 atacaacagg cgtcctctta tcaaaaatcc tggcgaacat ttccggttgc aggaaactgg
     1261 atccgtaggg cattttaggc acatttctta aagcaatata gctttccggt attgtatgag
     1321 catcaaacac acaatcggta accgcaaaag ccatcatatt gggcgggtga gatattataa
     1381 tactcttaac atgaggctgc attgcatata tcttaagatg cagtgccacc gaacgtgacg
     1441 gattctttcc cgcttctctg tatttgccgt tttcaattct tacaagatca tctactgtaa
     1501 tgtattttct gtctactccg tagggagtta ttacaaaggc atcatcgctt aatttgaccg
     1561 agaatgtccc ctgagtactg gtaaataact gttggtcgta agaacggtgt atcagttcgc
     1621 acatctctct tcttaccgcc ctctcttcgg aagaatagtg cgaaggaaca aactcctgca
     1681 tgggcagacc ttttttctgt aacgatatat ctatatgttt ttgcgacaac ggttttatat
     1741 tccctataca ttttgccctt atttccagtt tcgcacacat atcaatggtt tcaaacttca
     1801 taaaagcgtc aaaaagatcc ttaccgatta cgataacacc gtgattttcc atcattacgg
     1861 tatcatatcc ctttgcaaaa gccgcggcta tgttttcacc caaagcttca ctgccgggta
     1921 catcatattc cgccattccc acctctccgc atattctgtt tatattaggt acaaggtgga
     1981 cattgggtat ctgacggaca atactgaaag ctaccgaagc cggcggatgg gcatgtatta
     2041 cggcctttgc ttcggggcgt gtcctgtata catgcttatg gaacggaagc tctaccgacg
     2101 gcctgtgagg acctattatt gttccgtcgg gctttacctg cattatatct atccttttaa
     2161 gacttccttt atctattccg ctgggagtaa tccaaatatc gccgttttca tccagtaccg
     2221 aaagattacc gccgctggta gtggtcattc cgtacctgta tattctgttc atcagcataa
     2281 caatctgatc ggcaggatgc aataaattgt atttaatcat atcaaatatc ctttcctttt
     2341 atcattatct caatatgtgt aattgtaaag gtcttcgtgc gggaccatat ctgcagaata
     2401 ttgatatcgt ttatcatatc tatatcgggt attttgcata tatatcttcc gtcctttatg
     2461 taatagtccg gctgttccgt cttcttcaac cctttgacct gccggctgtt gaggtatatg
     2521 agaaggtcat catcgttcag cacatcatca caatctatac ccatctgaat atatgccgcg
     2581 gaattcttat cggtcttccc cgtccttatc ctgaatatta ccggttcgtt gctatcggga
     2641 ttgcaaagtg ccggtacata atacgcgctt ttttcccagg gagcggtaaa atccttgaaa
     2701 gtgacaagat gccttctgtc aagcgaaatg gaattttcca gactgttgca agctttaaga
     2761 atatccttaa tatctttgtg cagattatat aaagcggtat ctttaggatc ggttccgaaa
     2821 caatcgggca ttgtcatata gttaaaaaga taaaacttat ccgccccggc actgaaatat
     2881 gcagcactgt tggcatacac cgtttgctta caattgtatg tatagggaac ttccctgttc
     2941 tgtctcatca gcacttcaat tcccgcagca acggtcacat tatacggctt taacaacttc
     3001 ttccatactt caacgggcat gtcggtttcg gttgtcgacc atctgggagt tatgactatg
     3061 ttgtttataa ggccatgttc cgcccagccg gcaacatcca gtccgagata atagcaggtt
     3121 tccaccccgc tgggtactct aacggatacg gatataccgt gaccccgttt ttgctcatat
     3181 tttttgacaa tcttttttac atccttcata aaaccggtca gtatggtaat accttcctgt
     3241 tccataccgt atctgaacag ataagcttcc ctcatccagt ccagttccag tccgggcaca
     3301 tcataacgac ccagcgtttc gtcaataaaa tccagaaacc gctttcttat cgccgcctgt
     3361 ccgtaatcat aaagaccgtc atagtacaac tgaaccggac tgtatgtcac tcttctgtat
     3421 tgcggattgt cataatagaa atccggtgca atacaggatg ttttcttcat ggtctcatga
     3481 gcatcattca tccttattga tatccagggt ttgatattca tctttttaag tttatctatc
     3541 catatctgaa aatgatctat tccccattgg ttaaatattt tatcgtaagc cgatacccat
     3601 gtctttttga aatccacctg ctcaccgttc tgaacttttc ttctatatga atcgccgtaa
     3661 ttttcaatag tcactgacgg gtaagatgcc agtctcgcat taacgcacat tactatatcc
     3721 gttacattgg tcccctgata ctgacccatt acagcttccg tatatgcgat tgcttcctgt
     3781 ttatctttaa cacttttccc gtatcctttg tgaattccga agaagaatgc actgtcatcc
     3841 caattcaaca ttataccgct catatcatcc ctcctataca gtcgattata ccatgaatac
     3901 attacggaat aattgaaaac aacttattta ttcgtaaaaa atatacctta cgcttatatg
     3961 tattgataat atgctttatg aataagaact gagagatata tccgttaccg ggccaagtaa
     4021 aatatgttta tgtcaatttt atatttattt gacataaata ttacctgaat ccactataat
     4081 taatatacta atcccacgtg aaggcgagga aaaaatggag aaaaagggac ttaatgcaaa
     4141 tcatctgaag ttaatagcaa ttatcgcgat gacagtagac catatcgcag atctttttta
     4201 tccgggcttt cctgtgaagc ccttgcctat catacttcat ctgatcggca ggctgacagc
     4261 tcctatcatg tggttttttg tctgtgaggg tttttattac acaaggaatg tgaagaaata
     4321 tatgctcaga atgttcatat ttgcggtcat atcgcatttt gcatattgct tttcattcgg
     4381 cattgatccg gtgcctttcc gttcgggaat ttttaatcag acaagtgtta tgtacccgct
     4441 gtttatttcc gttgtagtgc tgtggttgga gcatgaagaa aagcgtatga acaaatatct
     4501 gaaacacata ataatatttc tgctcatatg cagtgcattc cccgctgact ggagctgtat
     4561 agcggtgctg gcaatcgtca gtatgtataa aaagcgcgga gatttaaaag gacagatgaa
     4621 aatgatgctt ctgtgggtgt ttttctacgg attgatatcg ttcttttttg tcagcaaagt
     4681 gtatgcactt gagttaatcg gtgttctcat ggtttatccg gtgttaaaaa tgtataacgg
     4741 tcaaaaagga aaagccggct ggatgaaaat gtttttctac atttattatc ctctgcattt
     4801 agttgtgata ggaataatac gcatattgat gtacggagat gtaccgttac tgttttagtt
     4861 tgataccctt gctttttaat ttaccaacat attactgcat ttacacttgt aaatattaaa
     4921 gaattcacta aacttatatt atgctctccg gtttttagag caaaaacaaa aaaatgtcct
     4981 cacggactga agaaagaata tgaaatacag acttatcgca acggatatag acggtacact
     5041 tttggatgat ttcggcatgc tgccggaaaa cacttatgca gcagccgctt atctggaaga
     5101 gaaaggtata aaacttgtac tgtgtaccgg aagacctctt ttaggtgtat taaagatata
     5161 tgatgccctt aagcttacat ctcccgttgt tacaaacaac ggagctatgg tttatctcgg
     5221 taaagacgga cctttaatat acagtgagac catgagcatt gaagacggct tgaatgtgat
     5281 taaactcaat gatattttac aatgcaccgt gtgcatgtgg cataacgggg tgttatacgt
     5341 atcggaactt aacaaatatg catacgatta tgcagccatt accggtatca ctcctcttct
     5401 tctgaacaca atagatttga gaaaatgtaa aaacggacct acaaagttcg tattcataga
     5461 tactttggaa aaaataacgg caattaagga gatggcagca aggcttgccc cttccaccgt
     5521 taccgcagtt acaacacgcc cctattttct ggagtttttc agcagcagta tttccaaagc
     5581 caccgctctt tccaaactgt ccgaagcatt gaatataggg cgcgagcaga tgattgctgc
     5641 gggagacggc tttaacgaca tcgatatgct ggattatgcc ggatttgcgg tggtagcagg
     5701 taatgccgac gaaggagtta aagaacatgc cgacatgatt gcgtgctcca acaatgacgg
     5761 tgttatagcc tatatagtag acaatcttat taagaaatct taaatgtctt tatctcatac
     5821 ggtcttatgg taaacttgag aacattgtcc tctattaccg catcggctat atcgttttcc
     5881 atgaggtcgc attccgttat tttggaaact tcaaaattga gatcgattgt acactcggta
     5941 cgcctgttaa agcactcata caccctcaaa atcagaccgt cgccctcttc ggctttcttt
     6001 aatgtatcga caatgacatt ctcatggtta catgtcataa aggatctgta attttctata
     6061 tctcccgtcc ctttttccac atactttgca taaggacgga agttgagttt gtatgccata
     6121 tccactgtat gggctttttt gaaatctccc atgtgaggat agagtgaata ggtgaactca
     6181 tgtaatcctc tgtcggcatc gggatcgggc tctgttgccg atttgagcag tgtcagcctt
     6241 ataacggaat cttttatgtc atgaccgtac ttgcagtcat tcagaatact tacaccgtag
     6301 tcatcttccg ataaatccgc ccatttgtga gcacatactt caaaacgagc ataatcccag
     6361 cttgtgttcc agtgggtagg tctcctgaca ttaccgaact gtatatcata agtggcataa
     6421 tcggtatgta catcaacggg gaaagcggct ttaagcagta aatcatcatg attacagtca
     6481 ataaccgtct taaaatcaat ctttcgagaa ttgcactcca gtgaaatata ctgtgtaata
     6541 gttgtttcca atatttttct gtccactctt atgcattttc ttaccggacc gtcttccact
     6601 aaagcagagt atgtaacatc attaatctca taaggtttct cattgtaata tatgtttata
     6661 tcccatgcct gatatgtcaa aggtttgtcc tcgaatgccg ttattttgtt tgcgg
//
LOCUS       DAOSOM010000068         6706 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_11463_length_6706_cov_5.995640, whole genome shotgun sequence.
ACCESSION   DAOSOM010000068 DAOSOM010000000
VERSION     DAOSOM010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6706
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11463_length_6706_cov_5.995640"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..1133
                     /locus_tag="PLF14_09625"
     CDS             63..1133
                     /locus_tag="PLF14_09625"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT71578.1"
                     /translation="MKEMTSKERFGRMFQHREADRIPIIDSPWEGTIRRWQTEGMPKD
                     TDWRDYFGVDKVSTINVDISPQYEVRVLEENEKQITYTTNYGVTLRKFKEADSTPEFL
                     DYKVDNSKIWFEETKKRMEEGTDRINWERYGRVYPEWVADGHWIQAGFWFGFDVTHSW
                     MCGTETILIAMLEQPEWVTDMFDTYLNSCIKHFDILWDKGYRFDSIFWYDDMGYKNKT
                     FFSLDTYRELVKPFHKKAVDWAHNKGIYAHLHSCGCVMNFVEDLVEIGVDALNPLEIK
                     AGMDPILLKKKYGDKLVLHGGINAVLWDKVDEVTEAIIQNVPVLKENGGYIFSSDHSI
                     PNTVSLENFTNIIKTVKEAGKY"
     gene            complement(1240..1776)
                     /locus_tag="PLF14_09630"
     CDS             complement(1240..1776)
                     /locus_tag="PLF14_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006283330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOT71579.1"
                     /translation="MKNLKGTKTEANLLEAFAGESMARNKYSYFASKAKKDGYVQIAN
                     IFEETANNEKEHAKIWFKLLEGIGDTPVNLEHAAAGENYEWTDMYPRFAKDAKAEGFK
                     EIAVLFEQVAKIEKDHEERYRKLLANINDGIVFSRDSETIWECTNCGHVVIGKKAPQM
                     CPVCDHPRDYFEIKVVNY"
     gene            complement(1773..2174)
                     /locus_tag="PLF14_09635"
     CDS             complement(1773..2174)
                     /locus_tag="PLF14_09635"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOT71580.1"
                     /translation="MKITRNTVQREIILQTVLGMDNHPSADEVYTKLRPENPSISRAT
                     VYRNLELLARQGKILHIQTPRGADCFDFNTDEHHHLRCEVCRRVYDINISDIPKIDYD
                     KIDSEGCIINGYNISFYGICPECQKNERSNT"
     gene            complement(2261..3163)
                     /locus_tag="PLF14_09640"
     CDS             complement(2261..3163)
                     /locus_tag="PLF14_09640"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT71581.1"
                     /translation="MKRIDMHIHSVMYIDDWEKQNDIPRVYRNETFATPDEIKPYYDK
                     LGIEKGVLLPVVSMEGQHRLINNEETYRIVRAYPAMFDWYCNIDPRWHYNSEDTDLGF
                     FIEHYKKLGAKGIGEVTSNLMFDDCRMRNLFYHAEKLSMPILFHLATEKEAYGIIDKP
                     GLPLLEKTLNDFPDLKFIGHSASFWSHISGDVTEETMHLYNKGPVVPGGSIPRLLRKY
                     GNLMCDISANSGYTAITRDPDFGYAFLEEFQDRIFYGTDICAPREYGFFDTVNFLDEG
                     YNNKRLSKSAYEKINRLNAEDILK"
     gene            complement(3174..4082)
                     /locus_tag="PLF14_09645"
     CDS             complement(3174..4082)
                     /locus_tag="PLF14_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71582.1"
                     /translation="MKKIDIHVHSRIYLSEEEEKNAIYRPIQDKLTYPAPEEIAILYE
                     KLGIEKGVLMVTIAPEGAHYTFNNLEAMRIVRKYPDRFYWFCGIDPRCWTNSADTDLS
                     HFINHYKKHGAKGIGEVTAKLPFSDERMQNLFRHAEMYDMPVLIHIGTDDSPYGIYDV
                     KGLKQLEETLTRFPKLTIIGHSQPFWAEIGADSEECDRFGYPSGPVKKAGRLVELMRK
                     YDNLHADLSANSGYNAITRDPDFGYEFLEEFQDRIYYGTGICSKREEKSFKLAQFLEQ
                     GAEKNKLSDRAYGKICRTNALRLLER"
     gene            4303..5145
                     /locus_tag="PLF14_09650"
     CDS             4303..5145
                     /locus_tag="PLF14_09650"
                     /inference="COORDINATES: protein motif:HMM:NF015951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HOT71583.1"
                     /translation="MKEKKPVPIAEWILLVIKGIVVGAGAIIPGVSGGVLCVVFGIYQ
                     PMMALLAHPIKSFKTYYKIFIPFIIGWLAGFLLIAKLVEMLFAASASIAIALFVGLIA
                     GTLPSLFKEAGKFGTDNKSRIGFTLGLFLMFTLLLLLDNQSSSSITPNIWWFAFSGLI
                     WGFSLIVPGLSSSSILIFMGLYQPMTSGIAALDMAVILPLIAGLAVTVLLFARLVNHL
                     FEKHHSIMHHTIIGIVIASTLLIIPTEYPNWWSLVISVVCFAAGFAASVFMNKLDKNS
                     EKTA"
     gene            complement(5173..6186)
                     /locus_tag="PLF14_09655"
     CDS             complement(5173..6186)
                     /locus_tag="PLF14_09655"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT71584.1"
                     /translation="MRQPDFDNIVKVLKRQTPDRPTLFEFYINNKTSEILSGRKSQSG
                     WDASWNYEMNIEAMSAAGYDYITLHGSNFSFPHFPKGQGYPIYDAASFSAYKFMDPDK
                     ANYKRLEECEKILPKKMKVIVFGPGGILENIVELVGYENMCIMIYEEPALFEKILDEV
                     GSRLVRYYENCLKYDCVGAVMVNDDWGFKTATMLSPEDLRKYIFPYYKKIVEMVHKSG
                     RLAMLHSCGNLTEVMDDIIYDMKFDGKHSFEDNILPVEEGYRKYADKIAILGGIDLDF
                     IARESVENIRARCAALIQLTGYKGYALGTGNSIPDYIPFKNFAAMIGCVYPEFEEHFS
                     HLL"
     gene            complement(6248..>6706)
                     /locus_tag="PLF14_09660"
     CDS             complement(6248..>6706)
                     /locus_tag="PLF14_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HOT71585.1"
                     /translation="PGMSMGHSVVARSVEGVKNALSMTIPLGTGVHRRMVYVELKDGA
                     DFAKVSKLIKEDDYFIHDETHVIQVPDVSALIDKGHGVNITRKGVSGITQNQNIGFRM
                     SINNPALTAQILVSCARAVVKQAPGCYTMLEVPLIDMLSGDKESLIHRLV"
BASE COUNT         1874 a         1441 c         1224 g         2167 t
ORIGIN      
        1 agcgaaatat acaagaagga ggagtatgaa cagtcactga ctgtatcata caagtaatcg
       61 gtatgaaaga aatgacatcg aaagaacgat tcggaagaat gtttcagcat cgggaagccg
      121 acagaatccc tataatagac tctccgtggg aaggtaccat aagaagatgg caaacagaag
      181 gaatgcctaa agataccgac tggagagatt atttcggtgt agacaaagtc tcgacaatca
      241 atgtagatat atccccgcaa tatgaagtca gggtactgga agaaaacgaa aagcagatta
      301 cctataccac caattacggc gttactttaa ggaaattcaa agaagccgac tccactccgg
      361 aatttctgga ttataaagtg gataattcaa aaatctggtt tgaagaaaca aaaaaacgaa
      421 tggaagaagg cacagacaga ataaattggg agcgctacgg gagagtatac cccgaatggg
      481 tcgcagacgg tcattggata caggcgggat tctggttcgg atttgatgtt acccattcct
      541 ggatgtgcgg tacagaaaca atactgatag caatgctcga gcaacccgag tgggttacgg
      601 acatgtttga tacttactta aattcctgca ttaaacattt tgatatactg tgggataagg
      661 gatacaggtt cgattccata ttctggtatg acgatatggg atataaaaac aagacatttt
      721 tctctttgga cacctacaga gaattagtga agccttttca taaaaaagct gtggattggg
      781 ctcacaacaa aggaatatat gcacatttac attcctgcgg ttgtgtaatg aacttcgtgg
      841 aagatctggt ggagatagga gtggatgctc ttaatcccct ggaaataaaa gccggaatgg
      901 atcccatctt attaaaaaag aaatacggag acaaattggt actccacggc gggataaatg
      961 cagtactctg ggataaggtt gatgaagtga ccgaggcaat tatacaaaat gttcccgttt
     1021 taaaggaaaa cggcggctac atattttcat cggaccattc catacccaac acggtcagtc
     1081 tggaaaactt tacgaacata ataaaaaccg ttaaagaggc gggaaaatat taaggatata
     1141 ctcctttgta gcataagtaa aataattgat taaactggaa aaaaagacag caacggctgt
     1201 cctttttgtt gtttcaatat tatactcgga aattaacggt taataattaa caaccttaat
     1261 ctcaaagtaa tctctcggat gatcgcatac cggacacatc tgaggtgctt ttttcccgat
     1321 aacaacatgc ccgcagttgg tgcattccca gattgtttcg gaatctcttg agaaaacaat
     1381 accgtcattg atattggcaa gcagttttct gtatctctct tcatgatctt tttctatctt
     1441 tgctacctgc tcaaaaagaa ctgcaatctc cttgaatcct tcggcttttg catcttttgc
     1501 aaatctggga tacatatcgg tccattcgta attctcgcct gctgccgcat gctcaagatt
     1561 aaccggtgtg tcgccaatac cttcgagcag tttaaaccat atttttgcgt gttctttctc
     1621 attattagct gtttcttcaa aaatattggc tatctgcaca tatccgtctt tttttgcttt
     1681 ggaagcaaaa taagagtact tgtttcttgc catggattcc ccagcgaaag cttccaataa
     1741 attagcttcc gtttttgtac cttttaaatt tttcatgtat tactcctttc atttttttga
     1801 cattcaggac atattccgta aaacgaaata ttatatccgt taataatgca tccctccgaa
     1861 tcaattttat cataatcaat cttcggaata tcggaaatat ttatgtcata tactctccga
     1921 catacttcac aacgcagatg atgatgctca tcagtgttga aatcgaaaca atccgcacct
     1981 ctgggtgtct gaatatgcag tattttcccc tgccttgcca gcaattccaa attcctgtaa
     2041 accgttgctc tgctgattga cgggttttcc ggtctcaact ttgtataaac ttcatcggcg
     2101 gacgggtgat tatccatacc caaaacggtt tgtaaaataa tttctctttg tactgtattt
     2161 cttgttattt tcataagtta caatatccta tctcattaag attatacaca tttatcgttt
     2221 tttggcaata tgtaaacccg aacaatttac ctgaataccg ttattttaat atatcttcgg
     2281 cattcagtct gtttattttt tcatatgccg atttgctcag gcgtttgtta ttgtatccct
     2341 catccaggaa gttaaccgtg tcaaagaaac cgtattccct cggagcacat atgtcggtcc
     2401 cgtagaaaat cctgtcctga aactcttcca agaatgcgta tccgaaatcg gggtctcttg
     2461 ttatagcggt ataaccgctg tttgccgaaa tatcacacat cagattccca tactttctca
     2521 aaagccgagg tatacttccc cccggaacaa ccggaccctt gttgtataag tgcatggttt
     2581 cttcggttac atccccgctt atatgcgacc agaaggaagc ggaatgccca ataaatttaa
     2641 ggtcgggaaa atcattaaga gtcttttcca gtaaaggaag acccggttta tcaataatac
     2701 cgtatgcttc tttttccgta gcgagatgaa aaagaatagg catgctcagc ttctctgcat
     2761 gataaaacag atttctcatt ctacagtcat cgaacataag attgcttgta acttctccta
     2821 ttcctttggc tcccagcttc ttatagtgct caataaagaa ccccaaatcg gtatcttccg
     2881 agttgtaatg ccatctggga tctatattac agtaccagtc aaacatagcc ggatatgcgc
     2941 gcactatcct gtaagtttcc tcattattta tcaatctgtg ctgtccttcc attgacacta
     3001 ccggtaaaag aacccctttt tcaataccca acttatcgta ataaggcttt atctcgtcgg
     3061 gtgttgcaaa tgtttcattc ctgtatactc tcggtatgtc attttgtttt tcccaatcat
     3121 ctatatacat gacggaatga atatgcatgt caattctttt catgttttcc tctttacctt
     3181 tccaaaagcc ttaaagcatt tgttctgcat attttcccgt atgccctatc gctcaatttg
     3241 ttcttttccg ctccctgttc caaaaactgt gccaatttga atgatttctc ctctcttttt
     3301 gaacagatac cggtaccgta gtatatcctg tcctgaaatt cttctaagaa ttcgtatccg
     3361 aaatcggggt cccttgtgat tgcattatat ccgctgtttg ctgacaggtc ggcatgaaga
     3421 ttatcgtatt ttctcataag ttccaccagt cttcccgctt ttttaaccgg tcccgaagga
     3481 tatccgaatc tgtcacattc ctccgaatcg gcacctattt ccgcccagaa cggttgagag
     3541 tgcccgatta tagtaagttt gggaaatctt gtcagagtct cttccagctg ctttaatcct
     3601 tttacatcat aaataccgta agggctgtca tccgtcccta tatgaattaa aacgggcata
     3661 tcgtacatct cggcatgtct gaacagattt tgcatacgct catctgaaaa aggcaacttt
     3721 gcagtcactt caccgatacc ttttgcaccg tgctttttat aatgatttat aaaatgtgaa
     3781 agatcggtat cggcactgtt tgtccagcat ctgggatcta ttccgcaaaa ccagtaaaag
     3841 cggtcgggat attttcttac tattctcatt gcttccaagt tgttgaaagt ataatgtgca
     3901 ccttcgggag ctatagttac cataagtact cccttttcaa tgccgagctt ttcatataat
     3961 atcgctattt cttcgggagc aggatatgta agtttatcct gtatcggtcg atatatagcg
     4021 tttttttcct cttcttccga cagatatatc ctgctgtgaa catggatatc aatttttttc
     4081 atatttttcc tttctaaaaa catatgcagc ggttctattc ttaataaacc gttataatga
     4141 atgccaactt catatattct aacacattaa tctcatcaat agccttaaaa ttattaatta
     4201 tttaaaactc tgtttattta ttcatttttt aataatatat cttcattgag attttaattg
     4261 cagatggtat aattcatgta ttaatatcga aaggactggg ttatgaaaga gaagaaacct
     4321 gttccgatag cggaatggat attgcttgtg ataaaaggaa tagtagtggg agccggtgct
     4381 ataattccgg gtgtaagcgg aggcgttcta tgtgtggttt tcgggatata tcagcccatg
     4441 atggctcttt tagcacatcc tataaagtcc tttaagactt attataaaat atttattcct
     4501 tttataatag gatggcttgc cggattttta ctcattgcaa aacttgtgga aatgctgttt
     4561 gcggcatccg cttcgatagc tatcgcactg ttcgtgggat taatagcggg aacattgccc
     4621 tctctgttta aggaagctgg taaattcggc accgacaata aatcccgtat aggctttaca
     4681 ctgggcttgt ttttaatgtt caccttatta ttgctgctgg ataatcaatc gtcctcgtcc
     4741 ataaccccga acatctggtg gtttgctttc tcgggtctta tatggggatt cagccttatt
     4801 gtacccggat tgagttcctc atccatctta atattcatgg ggctgtatca gccgatgact
     4861 tccggtatag ccgctttgga tatggcggta atactgccat tgatagccgg tcttgcggta
     4921 accgttcttt tatttgccag actggttaat catctgttcg agaagcacca cagtataatg
     4981 catcatacaa taataggaat agttattgca tccaccctcc ttataatccc taccgagtac
     5041 ccgaattggt ggagtcttgt tataagtgta gtctgctttg cagcaggctt tgcggcttcc
     5101 gtttttatga ataaattgga taaaaacagt gaaaagaccg cttaatcaaa tatattaaaa
     5161 tcagcattca ctttataaca aatgcgaaaa gtgttcttca aattccggat aaacacatcc
     5221 gatcattgct gcaaaatttt taaacggtat ataatcgggg atactgtttc ccgttcccag
     5281 tgcataccct ttatatcctg taagttgtat caatgctgcg catctggctc taatgttttc
     5341 caccgattcc cttgcaataa aatccaggtc aattccgccc agtatggcta ttttatccgc
     5401 atatttcctg tatccttctt caacaggcag aatgttgtct tcaaatgaat gtttcccatc
     5461 aaatttcata tcataaatta tgtcatccat cacttcagta agattaccgc atgaatgcag
     5521 catggctaaa cgaccgcttt tatggaccat ttccactatc tttttgtagt acgggaatat
     5581 gtattttctc aaatcttccg gtgacaacat ggtagcggtt ttaaagcccc agtcgtcatt
     5641 taccattacc gcacctacac agtcatattt taagcagttt tcataatatc ggaccaatct
     5701 gcttccaact tcatcaagta tcttctcaaa gagtgcaggt tcctcatata tcattatgca
     5761 catgttttca tagccgacaa gttctacaat attttctaaa atcccgccgg gaccgaacac
     5821 tataactttc atttttttag gcagtatctt ctcgcactct tccagccttt tatagtttgc
     5881 tttatcgggg tccatgaatt tatatgctga aaaagatgcc gcgtcatata tgggataccc
     5941 ctgccctttg ggaaaatgag gaaaggagaa attgctgccg tgtaaagtta tatagtcata
     6001 tccggcagcg gacatggctt ctatattcat ctcataattc catgaagcat cccatccgct
     6061 ttgacttttc ctgcctgata atatttccga agtcttgttg tttatataaa actcaaacaa
     6121 ggtaggcctg tcgggagtct gcctttttaa tacttttacg atattgtcaa aatccggttg
     6181 tctcatatat gagtttcctc tactatatgc tacatagtat atttattgcc acatgcttat
     6241 cgttaatcta taccaacctg tgtataaggc tttccttatc tccgctcagc atatcaatca
     6301 gcggtacttc cagcattgtg taacatcccg gagcctgttt cactacggcc cttgcacagg
     6361 ataccaatat ctgcgccgtc aatgcgggat tgttaatgga cattctaaac ccgatattct
     6421 gattctgagt aataccggaa acaccttttc tggttatatt cactccgtgt cctttatcta
     6481 tcagtgcact tacatcgggt acctgaataa catgcgtttc atcatgtata aaatagtcat
     6541 cttccttaat taactttgat acctttgcaa aatctgcacc gtctttaagc tcgacatata
     6601 ccatccttct gtgtactccg gtacctaacg gtatggtcat ggacagcgca tttttaactc
     6661 cttcaacact ccttgcaaca accgaatgcc ccatgctcat cccggg
//
LOCUS       DAOSOM010000069         6482 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_12001_length_6482_cov_5.796172, whole genome shotgun sequence.
ACCESSION   DAOSOM010000069 DAOSOM010000000
VERSION     DAOSOM010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6482
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12001_length_6482_cov_5.796172"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1150
                     /locus_tag="PLF14_09665"
     CDS             <1..1150
                     /locus_tag="PLF14_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71586.1"
                     /translation="NDKGNVIKINGKNIDYAKTQGVRCTVDNNLVKLKTGQDIDLLKK
                     SEDSKTRIPSYKLPVTVLSIDNLREPVIADSFISSIRVNESLEYQFEFPYIIKDGKKQ
                     IVLYEADFSLIKIPNSDKYIVEKGGLLFLLDPEAESLQFYCLTETKEYSQYNTKSFNG
                     IEYAVVWAVRPSFNEDGTKMIYYSERATEKVGYVWVKDTVTGKENPVPNTKGYSEVLQ
                     WRDNRYAYLLCQGRIIEIDTAELSSKLIYDTGNPGNMTLGFVYPHVFIPGGLNCKSQV
                     VNILNNEVTLFDDTKYSGCMSIHAMDNINKLFICYSQPKDVNLFHNEIVVLDMETMQK
                     ATISIEDEYSFLMIQPYKSDRFLLNYSKGSDIYNQETACISYDKLFAR"
     gene            1392..3038
                     /locus_tag="PLF14_09670"
     CDS             1392..3038
                     /locus_tag="PLF14_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71587.1"
                     /translation="MKKRFIVQLVAIFVLLSVLVAPVFTGYANTDVKNTKAQTQNVKR
                     PETDFILDFSKDKFDKVEAFVDGNYVDITDCWNSFQRFISASWFMYYTPEKRVLATEP
                     RIDENSKCIKFTKDNEVYYGTAYPLTHNAFGGYDGFHVWHDHVMYRYAVSNITLFHRA
                     FDYIGYWEINNWTNQKNTIIKTSVYDMMCDSPLLIRKMNRSGDLYYWGQEKYAVVSAI
                     ANHYTKTLEQKPDNLIKYGIRRHYTYVYSSITEIDVTLYAQSLWQKGLYAHIRMNDEE
                     VWLVLDTCVKDIQAELNNCDYYKVPALTKDMAALKLNGYDKVTLINNDKEYDITNMWF
                     VNFDNKGLCDWTEYTKAAPLKTDCKIILEKEGEKLVMNVARTYEYAVIEGKGLYVLPH
                     IESIIDAVLQSDLHRAVDTLVKKSVLYKTYRSSFVFRRFFSEESKGYFYEETFQPANS
                     LIIRYAETLPGKPDIETYKWVEKDFLYKADSIKMTVWRIDDYGDVGYVNLSGEGADIW
                     YKIQDAYMMFSYNGCDAVGDPWVRLNFLPEYTMVPDSISK"
     gene            3083..5713
                     /locus_tag="PLF14_09675"
     CDS             3083..5713
                     /locus_tag="PLF14_09675"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOT71588.1"
                     /translation="MKRIISILLILALLLALSSCDTVPTDTPIPSETILATETAVVPS
                     DTPTVEPSQTTQPTPTPTPTPTPTPTPTPTPTPTPTPVPTPTPTPIPTPTPTPTPVPE
                     KPSAYTDIKYFTFDARHRTILDYNPEGGAYVNIPDKIRGIPVEKIGKHAFAYKDLYGA
                     VIPDTVKVIGESAFANNRYMVYFTELTLGKNVEVIEDYAFDYCQIKSLVLPDKVKYIG
                     KCAFSGCTSDVVIPDSTEYIGVGAFNSLNQSNITLGNGVTSYYVVEGDTFAGYICKPE
                     NNCVIPSIPGVDTIKDESFLWHSLWSITVPEGIKRIGNRAFNRAAKNITLPNSVISMG
                     ECVFSGDTVITCEPGSFAHYYAKKMRVALSDSVVPTSFPISREAIISTDISEISKKDY
                     SYPFENWIRLYVNSKYGSYGIKHLLIKTCEYSDIDARLYDLTNSLFSDEYNVAAKYYY
                     VTFKTDKDIPDAEKGNAGYYRFDIQVVALYNADGKVILSGFVECRENLIDNNEKLYDF
                     IKQDTRFVFKEYSAPASILNSQAVEIINVSDYSVVNDIVKNINFAITGDKEGYVPISR
                     DIVAFFLRKFVPTPTVYMANLKTGEILFSKEYPYYTFLSSKDGVCVIQYSTDGIYSYD
                     YVNDKGQIINQITGGIDYYLDMPKETKGSSYRLIQENRSIRIKNMTIGASNMLLMFKQ
                     IDFHKWLDYRLGEVIDSNRFIYTCYEMQGLAPGWNRTYIYDIRDGSSLEIKNSLKGNR
                     YHEYAGYYGGKYFVKFCNTFDAANQIYILETEGENAGNLECITENVDKCLAIKDYAIS
                     SDGKYLALLTSTKPSVEGSLNDFSEHIVIVDIPTKKVIYEDEYEQRSGSVTYTQDGYF
                     CINDGKKLIYIDTGKLSSGK"
     gene            6026..>6482
                     /locus_tag="PLF14_09680"
     CDS             6026..>6482
                     /locus_tag="PLF14_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71589.1"
                     /translation="MSKRETAKRTDTVRYLKWNNDIIGIIDETNAVTFTEPRYNKVVT
                     LYTHGASLWTPEQFTEFLTERTVSRDRRDIERILFRCGLSHYDVLRICEITRGIHPKD
                     LLWIANDEKETLDVVMTDVFSSVFLQRIDLTGDSIDTPEGYNIKRYGVFD"
BASE COUNT         2217 a         1030 c         1355 g         1880 t
ORIGIN      
        1 aaacgataaa ggaaatgtta taaaaataaa tggcaaaaat attgactatg caaaaactca
       61 aggggtcaga tgtactgttg ataataactt ggttaagtta aaaacagggc aggacattga
      121 tttgctgaaa aaatctgaag actcaaaaac tcgtatccct tcatataaat tacctgtaac
      181 ggttctgtct attgacaatc ttagagaacc tgtaattgca gattcgttca tttcttcaat
      241 tcgagtcaac gagagtttgg aatatcagtt tgagtttccg tacatcatta aggacggcaa
      301 aaaacagata gtactttacg aggcggattt cagtttaata aagattccta attcagacaa
      361 gtacattgtt gaaaaagggg gcttgctatt cttgcttgat cctgaagcag aatcactgca
      421 attttattgt cttaccgaaa caaaggagta ttctcagtac aacaccaaaa gttttaacgg
      481 tattgaatat gctgtggtat gggcagtaag accgtcattc aatgaagatg gaacaaaaat
      541 gatatactac tccgaaagag caactgaaaa agtcgggtat gtttgggtaa aagacactgt
      601 taccggaaaa gagaatcctg ttccgaacac aaaagggtat tcggaagtac tgcagtggag
      661 agacaacaga tatgcgtatt tgctgtgtca gggaagaata attgaaattg acacggcaga
      721 attgtcttcc aaactcattt atgacacagg taatcccggg aatatgacac ttggatttgt
      781 ttatccgcat gtttttatac ccggcggttt aaattgtaaa tcacaagttg ttaacatact
      841 caataatgaa gttactttgt ttgatgatac aaaatattcc ggatgtatga gcatacatgc
      901 tatggataac atcaacaaac tttttatctg ttattctcaa ccaaaagatg ttaatctgtt
      961 ccacaatgag atagtagtac ttgatatgga aacaatgcaa aaagcaacta tatccataga
     1021 ggatgaatat tcatttttga tgattcaacc atataaaagt gatagatttc ttttgaatta
     1081 tagcaaaggc agtgacattt ataatcaaga aacagcttgt atcagctatg acaagttatt
     1141 tgcgagataa acggggttag aaagaatatt attaaaacct tactattatc tatactgata
     1201 tgtattgaga aatagaggaa aaaaagagta attttcttga atttatgaac tttttgtaaa
     1261 caaagaacgg gaatttgtaa acaatttatg aacaaagcaa tttgctaatc ttaaaggtat
     1321 ataatagagc acagaagtta cttttagtta acatataata tttataatta ataaagaatg
     1381 tgaggtttga tatgaaaaag aggttcattg ttcagcttgt agcaatattt gtattactgt
     1441 ctgttttagt tgctccggtg tttacggggt atgctaatac cgatgtaaag aatacaaaag
     1501 ctcagacaca gaatgtaaaa agaccggaga cagactttat tttagatttt tccaaagata
     1561 agtttgataa ggtagaggct tttgtggacg gtaattatgt tgatattacc gactgctgga
     1621 attcctttca aaggttcata agtgcttcat ggttcatgta ttatacacct gaaaagcgtg
     1681 ttttagccac agagccgagg atagatgaaa acagtaaatg tataaaattt acaaaagata
     1741 atgaggtata ttacggtacg gcgtatccgt taacccataa tgcattcggc ggatatgatg
     1801 gttttcatgt ttggcatgac catgttatgt atagatatgc agtatctaat attacgctat
     1861 ttcacagagc ttttgattat atcggttatt gggaaataaa caattggacc aaccagaaga
     1921 acactattat taaaacaagt gtatatgata tgatgtgcga cagtccgttg cttataagaa
     1981 aaatgaacag atcaggtgac ttatactact gggggcagga aaaatatgcg gttgtgtccg
     2041 ctatagccaa tcactataca aaaacattgg agcagaaacc cgataattta attaagtacg
     2101 gaataaggcg tcattatacc tatgtgtatt catcaataac cgaaattgat gtaacgctat
     2161 acgcacaatc tctttggcag aaaggtcttt atgcccatat tagaatgaac gatgaggaag
     2221 tgtggcttgt tttagacact tgcgtaaagg atatacaggc tgaattgaat aattgcgatt
     2281 actataaagt gcctgctctt acaaaagata tggctgcact taaattaaac ggatacgata
     2341 aggttacatt aattaacaac gacaaagaat atgatattac aaatatgtgg ttcgtgaatt
     2401 tcgataataa gggtctttgt gattggacgg aatatactaa agcggccccc ttaaaaacgg
     2461 actgtaagat tattcttgaa aaagaaggtg aaaagcttgt aatgaatgtt gcaagaacat
     2521 atgaatatgc agtaatagaa ggcaaaggac tgtatgtttt accgcatatt gaatcaataa
     2581 ttgatgctgt tttgcagtcg gatttacaca gagcagttga taccttggtt aaaaagagcg
     2641 ttttgtataa gacatataga tcttcctttg tatttcgcag atttttttca gaggagagca
     2701 agggctattt ctacgaggaa acctttcaac ctgctaactc tttgataata agatatgcgg
     2761 aaacactacc tggaaaacct gacatagaaa catataaatg ggtagaaaaa gattttcttt
     2821 acaaagctga cagcatcaaa atgactgttt ggagaataga tgattacggt gatgtagggt
     2881 atgtaaattt gtccggagaa ggtgcggata tatggtataa gatacaggac gcatatatga
     2941 tgttttcata taacggttgt gatgccgtgg gtgatccgtg ggtgcgtctt aactttcttc
     3001 cggaatatac gatggttccg gacagtatta gtaaatagaa acggtcggat atatgaaatc
     3061 atgtggaaga ttggagaagg aaatgaagag aataataagc atattactga tattagcact
     3121 attgttggca ttatcatcct gtgatactgt gcctaccgat acaccgatac cttcggaaac
     3181 tattcttgcg acggaaacgg ctgttgtacc cagtgacacc ccgacggtag agccgagtca
     3241 gacaactcaa cctacgccta caccgactcc aactccaact cctacaccta caccaacacc
     3301 aacacctacg ccaacaccta caccggtacc gacacctaca ccaacaccga taccgactcc
     3361 gacacctacg cctacacctg taccggagaa gccgtctgca tataccgata taaagtattt
     3421 cacttttgac gccagacata gaaccatact ggactataat cccgaaggcg gagcttatgt
     3481 gaatattccc gataaaatac gcggcatacc tgttgaaaag ataggtaaac atgcatttgc
     3541 atataaagat ctgtatggag ctgttatacc cgatacggta aaagtaatag gggaatcggc
     3601 atttgctaat aacagatata tggtttattt tactgaatta acccttggaa agaatgttga
     3661 agttattgaa gactatgctt ttgattactg tcagataaaa tctcttgttc tgccggataa
     3721 agttaagtat ataggaaaat gtgcattttc cggctgtaca tccgatgtgg ttatacccga
     3781 cagcaccgaa tatatcggag taggggcctt taattcattg aatcagagta atattacatt
     3841 aggcaacgga gttacatcat actatgtggt agagggagat acttttgcag gatatatatg
     3901 taaaccggag aataattgcg taattccttc cataccggga gtggatacaa tcaaagacga
     3961 atcatttctg tggcattctc tttggagtat cacagtaccc gaaggcatca aaagaatagg
     4021 gaatagggca tttaacaggg ctgcaaaaaa cattacacta ccaaacagcg ttatatccat
     4081 gggagaatgt gtgttttcag gagacaccgt gattacttgt gaaccggggt catttgcaca
     4141 ttattatgca aagaagatga gggttgcttt atcagacagt gtcgtgccta catcttttcc
     4201 tataagcaga gaagcaataa tcagtaccga tatttccgag ataagtaaga aagattattc
     4261 ctatccgttt gaaaactgga taagactgta tgtaaacagc aagtatggtt cttatggaat
     4321 aaaacatctt ttaattaaaa cctgcgaata tagtgatatt gatgccaggt tgtatgattt
     4381 aacaaattca ttgttttccg atgaatacaa tgttgctgcg aaatactact atgtaacttt
     4441 taaaacagac aaagatatac ctgatgcgga gaaaggtaac gcaggttatt atagattcga
     4501 cattcaggtt gtggctctgt ataatgccga cggcaaggtt atactttccg gctttgttga
     4561 atgtagagaa aatctcattg ataataacga aaaattatat gattttataa aacaggatac
     4621 cagatttgta tttaaggaat attccgctcc tgcaagcatt ctgaattcgc aagctgtgga
     4681 aataatcaat gtttccgatt acagtgtggt caatgatatt gtgaaaaata taaattttgc
     4741 cataaccggt gacaaagaag gatatgttcc gattagtaga gatattgtag catttttcct
     4801 tagaaaattt gttccgactc cgaccgtgta catggcaaat ctgaaaacag gcgaaatact
     4861 gttttcaaag gaatatccgt attacacatt tttgtcgtca aaagacggtg tatgcgttat
     4921 tcaatatagt acggacggga tatatagcta tgattatgtg aatgacaaag gacagataat
     4981 caatcagatt acaggcggca tagattatta tttagacatg cctaaagaga caaagggatc
     5041 ctcatacagg ttgattcagg aaaaccggtc aatcagaatt aagaatatga caatcggtgc
     5101 tagcaatatg cttttaatgt ttaagcagat tgactttcat aagtggctgg attaccggtt
     5161 aggagaggta attgacagca acagatttat ttacacctgt tatgagatgc aaggacttgc
     5221 tcccggttgg aatcgtacat acatatatga catacgtgac ggaagcagtt tggagataaa
     5281 aaacagttta aagggaaaca gatatcatga gtatgcaggt tattacggag gcaagtattt
     5341 tgtaaagttt tgcaacacat tcgatgctgc caatcagata tatattttag aaaccgaagg
     5401 agaaaatgcg ggaaatcttg agtgcataac cgaaaatgtg gataaatgct tggcaataaa
     5461 agattatgct ataagtagtg atggcaagta tttagcatta cttacatcaa caaagccctc
     5521 cgtagaggga agtctgaatg atttcagcga acatatagtg atagtggata taccgacaaa
     5581 gaaagttatc tatgaggatg aatatgagca aagaagcgga agtgtaacat atacacaaga
     5641 cggttacttc tgtataaatg acgggaaaaa acttatatat atagacacag gtaagttgag
     5701 cagcggaaaa tgattgttta aaaggcttta gagggtttaa tgcttgcctg ttttataaat
     5761 gtaacaccta cacatcaatt gccggtgata atataactgt aaaaagagtt aagaaactgg
     5821 acttcaatat aataatagat atacaagtat gtataagtta ttggcagtat gttaaacagc
     5881 ttatccatga cttggagata tccgccgatg cgaacgaagt tttaagtatg agagacggaa
     5941 aactgattga taaatagctt ctttacgccc ccggatataa tcatgctata atatcattat
     6001 catatggtga aagggaggcg ttgttatgtc aaagcgggaa actgcaaaaa gaacagatac
     6061 agttcgctat ttaaagtgga ataatgatat tatcggcata attgatgaaa caaatgctgt
     6121 tactttcact gagccgagat ataacaaggt ggtgactctg tatacacacg gtgcctcatt
     6181 atggacacct gaacagttta cggaatttct tacagaacgc accgtaagcc gtgaccgccg
     6241 cgatattgaa cgcatactgt ttcgttgcgg tctttcacat tatgatgttt tgcgtatatg
     6301 tgagattaca aggggtatcc atccgaaaga tttactgtgg attgcaaacg acgaaaaaga
     6361 gaccttggat gttgtgatga cggatgtgtt ctcgtccgtc tttctgcaaa ggattgattt
     6421 aacgggagac agtatagata cacctgaggg ctataacatt aaacgatacg gcgtttttga
     6481 cg
//
LOCUS       DAOSOM010000070         5986 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_13392_length_5986_cov_8.521160, whole genome shotgun sequence.
ACCESSION   DAOSOM010000070 DAOSOM010000000
VERSION     DAOSOM010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5986
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13392_length_5986_cov_8.521160"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /locus_tag="PLF14_09685"
     CDS             complement(<1..1048)
                     /locus_tag="PLF14_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71590.1"
                     /translation="MRKRTELYLMRYGITNILRSLPKSIALVCAAMVLTLSICIISQS
                     IVTQTQSLEDVYSEYIIETVITDQFCIEETNLAMNSKYYNIISQNLHGIRDYITDIKI
                     RSSFSSNYTYMFDDKVERELGGSVVGLTYLPDEEEFKHEFNLMPLYDESILLDSRPSC
                     IVPKRMMEDMGWEYYTVRLRYDKQEENEFVEFNIVGTHEGPDNIYCSYESFNDELTKG
                     TVSIYSFRFNLINSKYTGEVAKILKFYFIEPSIVGAIRRELNELKILFRYTFVMPKGN
                     LDDVVKPIESDIRKLKIAEPFLFVLSVATGLVVSFMSTKNRKAEFAVMRSLGTGKAMV
                     FFEAFFEQLLLCLTG"
     gene            complement(1032..3008)
                     /locus_tag="PLF14_09690"
     CDS             complement(1032..3008)
                     /locus_tag="PLF14_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71591.1"
                     /translation="MKYSIKKLYRAPIKTAIFILLIALSASALSISMGMWKYSYDSIK
                     YSKDAFTTIGIVRELEFMEQIYTSSGEPKYDYELLGKVKQAAEDSQYTKHTDRRKYLM
                     GYSPDITSFASDGTRERFYPYPYGIITGVCESIGFTRGSNYAAIFKIDKEDLNILPYF
                     VERKDPNISNEYIYVRGLHLTNDLRFPFEVGEKYIVHVYFSGRDNDLKMYSGRLDYSG
                     RYSEIVKYGEFYNLSEEEKKERPEIDRDRVSRAYEDTVHPFQKLTSSAQALLDSGDKR
                     WNELVNNCRITKHSTEILLTDDMYSMYSFNTGDLYIVNGRAISKDEYEQGAKVCVISW
                     NVAVTNDLRVGDKIKLSVYESDFSVFNRHIPIGDRGSSADYITEDIFAPLGYRGQDFI
                     TEAEYEIIGEYRGKGALDRGEFLISHNMIIVPSKSLEGDFNTKPIIAETVRSVDGKSQ
                     FVKKERTSIPGSFSVVIENGKTEEFEKEMEALGYGGMFYYFDQNYSEIAGKLDGYMKT
                     ASFILFISVLAWVCIAAVFLSIYMINSRKNLATMLSLGAGRVRAFFSEMSGIVLIVII
                     ASVIGLIAGMELYGDVLDKTIAASFEGMSAPIELFKAMNISKMQSTKAVWLSAIIQAV
                     VLILIAAIIECTQSGQDVFRLMKGLKKSKNAKKN"
     gene            complement(3033..4097)
                     /locus_tag="PLF14_09695"
     CDS             complement(3033..4097)
                     /locus_tag="PLF14_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71592.1"
                     /translation="MMKNKLKFLLFILLLVILVGCNNMQPGPTDGISETEVIDDSFEG
                     ADAVTKDEGNIKYWMADINILIDILNEYYENELVMDFRYRSFSGTEIKPIESRYDNDT
                     VHGADGSIMSMTGLIQGIVSEKGNSICINIIEDKTFDNGGYIFSNISSFSYDYFNNYG
                     SEKYTVSVYKYKNCYIVFEFFNSDLSPEDKTDIIENCVAVVEENYTEKKKVLFNENDD
                     FYIPKEHTQDYINNLKNALNTLYKDNLTSKFQKYTKSDVPLFEIRKKGESVLAETTYY
                     QGTILKDRKAIVSIAEDNVVENNGFIYTTATTDDTPGIQIYRYNKLYLIFEFYDDPAT
                     AFWNNDVVRSVAKVLSDLEK"
     gene            complement(4142..>5986)
                     /locus_tag="PLF14_09700"
     CDS             complement(4142..>5986)
                     /locus_tag="PLF14_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT71593.1"
                     /translation="KYSKDAFTTIGIVRELEFTEQIYVSSEEPKYDYELLGKVKQAAE
                     NSQYTKYTDRRKYLMGYSPDITSFIISGTRTRFYPYSYAIITGVCERIGFWRDSNYRA
                     VFKIDKNDLNIFPLFVEKPGPRYNSDYILIGGTLLTNDLRFPFKVGEKYIIHAYMFGW
                     DYEEGMFSGRLDYNGTLGPDVGVYGDLYTLSEEEKKERPEIDRDKVWRVFDDSTHPFQ
                     ALSTDAQSLLDSEDQKWNRLVDNCEITKHSMEILLTDDMYSMYPFNAGDSYIDRGRAI
                     SKDEYEQGAKVCVISAPVSVTNDLRVGDRIKLSVYESDFTVDSKMIIRKDTGQPESNE
                     DYFAPLGYRGQDFITEVEYEIIGAYTNKGSGDRGELTLSNNVLIAPSKSIEGDFNTKP
                     IVAELRRAGDGKVRSVKKERTSIPGSFSVVIENGKTEEFEKEMEALGYGGMFYYFDQN
                     YSEIAGKLDGYMKTASFILFISVLAWVCIAAVFLSIYMINSRKNLATMLSLGAGRVRA
                     FFSEMSGIVLIVIIASVIGLIAGMELYGDVLDKTIAASFEGMSAPIELFKAMNISKMQ
                     STKAVWLSAIIQAVVLILIAAIIECTQSGQDVFRLMKGLKKSKNAKKN"
BASE COUNT         1685 a         1389 c          912 g         2000 t
ORIGIN      
        1 tacctgtcaa acaaagcagc agttgttcaa aaaatgcttc aaagaaaacc atcgccttgc
       61 cggtacccaa acttctcata acggcaaatt ccgcttttct gttcttggtg gacataaaac
      121 ttacaactaa acccgtagca accgatagca caaacaaaaa aggctccgct atcttgagtt
      181 tcctgatatc cgattcgata ggtttaacca catcatcaag atttcctttg ggcattacaa
      241 aagtatatct gaacaatatt ttcaattcat tcaattctct tcttatcgca ccgacgatag
      301 aaggttcaat aaaataaaac ttcaatatct tcgcaacttc tccggtatat tttgaattaa
      361 taaggttaaa cctgaaggaa tatatgctga cggtgccttt tgtaagttca tcattaaaac
      421 tctcatacga gcagtagata ttatccggcc cttcatgggt tcccacaatg ttaaactcca
      481 caaactcatt ctcttcctgt ttatcatatc tcaagcgaac tgtatagtac tcccatccca
      541 tatcttccat cattctctta ggaactatac atgacggccg gctgtccaag agaatcgact
      601 cgtcatatag gggcatcagg ttaaactcat gtttgaactc ttcttcatcc ggaagatatg
      661 tcaaaccgac tactgatccg ccaagttccc gctccacttt gtcatcaaac atatatgtgt
      721 aattgctact aaacgaactt cttatcttta tatcggtaat atagtctctg atgccatgta
      781 ggttctgtga aattatatta tagtatttag agttcatagc caggtttgtt tcctctatgc
      841 agaattgatc ggttataacc gtttctatta tatattccga atatacatct tccaacgact
      901 gtgtctgtgt aactattgac tgacttatga tgcaaatgga taaggtaagt accattgccg
      961 cacaaaccaa agcaatactc ttaggcaaac tccttaaaat gttggttatc ccgtacctca
     1021 tcaggtataa ttcagttctt tttcgcattt ttcgatttct tcaatccttt cataagccta
     1081 aatacatcct gacccgactg ggtgcactct attattgcag caatgagtat caatacaact
     1141 gcctgaatga ttgcgcttag ccacactgct tttgtagact gcatcttgct gatattcatc
     1201 gctttgaata attcaatcgg tgctgacatt ccttcaaaac ttgctgctat agtcttgtcc
     1261 aaaacatccc cgtataattc catgcctgca ataaggccta ttaccgaagc tataataaca
     1321 ataagcacta taccgctcat ttcactgaag aatgctctaa ctctgcccgc acctaaggaa
     1381 agcatggttg caagattctt tctgctgttt atcatatata tacttaaaaa tacagcggct
     1441 atacataccc acgccagtac agatatgaat aatataaatg aggcggtctt catatacccg
     1501 tcaagcttac ctgctatctc gctgtaattc tggtcaaagt aatagaacat gcctccgtag
     1561 cctaaagctt ccatctcttt ttcaaactcc tctgttttcc cgttttcaat taccaccgag
     1621 aaagaaccgg gtatgctcgt tctctccttt ttaacaaact gtgactttcc gtctacggat
     1681 cttaccgttt cggctataat gggtttagta ttgaaatcac cttcaagtga ttttgaaggt
     1741 acaataatca tattgtgaga aatcagaaac tcacccctgt ccaatgctcc ctttcctctg
     1801 tattctccga taatttcata ctctgcttcg gttatgaaat cctgtcctct gtatcccaaa
     1861 ggagcaaaga tatcttcggt gatatagtcc gcagaactgc ctctatcgcc tatcggaata
     1921 tgtctgttaa atacagaaaa gtcactttca taaacagaaa gctttatctt atcgcctact
     1981 cttaaatcat ttgtaactgc tacattccaa cttattacac aaactttagc accttgctca
     2041 tattcatctt tgcttattgc cctgccgttg actatatata aatcaccggt attaaacgaa
     2101 tacatgctgt acatatcatc ggtaagcagt atttccgtag aatgtttggt tatcctgcag
     2161 ttatttacca actcgttcca ccttttatcc ccggaatcca ataaggcttg cgcactggaa
     2221 gtcagctttt gaaacggatg cacagtgtct tcgtaagcac ggctcaccct gtcacgatct
     2281 atttccggtc tctctttctt ctcttcttcc gagaggttat aaaactcacc gtacttaact
     2341 atctccgagt atcttccgct gtaatccagt cgtccggaat acattttcag atcattgtcc
     2401 cgccctgaaa aatatacatg aactatatac ttttctccca cttcgaacgg aaacctcaag
     2461 tcgtttgtaa gatgtaatcc ccttacgtat atatattcat tacttatatt cgggtccttt
     2521 ctctcaacaa aatagggcaa tatattcaaa tcttccttat ctatcttgaa aattgcggca
     2581 taatttgagc ctcttgtaaa acctatactc tcacacactc ctgtaattat tccatacgga
     2641 taaggataaa atctttctct ggtcccatca ctggcaaagc tcgttatatc cggactatat
     2701 cccatcagat attttcgcct gtcggtatgt tttgtatatt gtgagtcctc tgcggcttgc
     2761 tttactttac caagcaactc gtaatcatat ttaggctccc cggaagatgt atatatctgt
     2821 tccataaact ccaactctct tacaatacct atcgtggtga atgcatcttt tgaatatttt
     2881 atactgtcat aagaatattt ccacataccc atacttatac tcaatgcact cgctgaaaga
     2941 gcaataagca gtatgaatat agcggtcttt atgggtgctc tgtatagttt cttaatggag
     3001 tatttcatta ttacctcact tcttgtaatg ttctactttt ccaaatcgga taatacctta
     3061 gcaacagagc gaactacatc attattccaa aatgctgttg caggatcatc gtaaaactcg
     3121 aaaattaaat agagtttgtt atacctgtat atttgaattc ccggagtatc atcggttgtg
     3181 gcggtagtat aaataaagcc gttgttttca actacattat cttctgcaat agacactatt
     3241 gctttacggt ctttcagtat agttccttga taatatgtag tttccgccaa aacgctttcg
     3301 ccttttttcc ttatctcaaa cagagggaca tcggacttgg tatatttttg aaatttgctt
     3361 gtaagattat ctttataaag tgtatttaaa gcatttttaa ggttattgat ataatcttgt
     3421 gtatgttctt tgggaatgta aaaatcatca ttctcattga acaaaacctt tttcttttcg
     3481 gtataattct cttccactac tgctacacaa ttctcaatta tgtcggtttt gtcttccggt
     3541 gataaatccg agttaaagaa ttcaaacaca atatagcagt ttttgtattt gtataccgac
     3601 acggtatatt tctcagaacc gtaattatta aaataatcat agctgaagga cgaaatgttt
     3661 gagaatatat atccgccgtt atcaaaggtt ttatcctcta ttatatttat gcaaatcgaa
     3721 ttgccttttt ccgaaactat tccctgaatt aatccggtca tagacattat acttccgtca
     3781 gcaccatgta cagtgtcatt atcatacctg ctttctatcg gcttaatctc cgtaccgcta
     3841 aaggaacggt atctaaagtc cattaccaat tcattttcat aatactcatt cagtatgtca
     3901 attaagatgt ttatatctgc catccaatac tttatattcc cttcgtcttt tgtcactgca
     3961 tcggctcctt caaaactgtc gtcaataacc tctgtttcgg aaattccgtc agtgggaccc
     4021 ggttgcatat tattacagcc gaccaatatt acaagtaaca atataaataa caaaaacttt
     4081 aacttattct tcatcattta tcctttcaaa aaatcatgta tagtattaat agtgctgtaa
     4141 ttcagttctt tttcgcattt ttcgatttct tcaatccttt cataagccta aatacatcct
     4201 gacccgactg ggtgcactct attattgcag caatgagtat caatacaact gcctgaatga
     4261 ttgcgcttag ccacactgct tttgtagact gcatcttgct gatattcatc gctttgaata
     4321 attcaatcgg tgctgacatt ccttcaaaac ttgctgctat agtcttgtcc aaaacatccc
     4381 cgtataattc catgcctgca ataaggccta ttaccgaagc tataataaca ataagcacta
     4441 taccgctcat ttcactgaag aatgctctaa ctctgcccgc acctaaggaa agcatggttg
     4501 caagattctt tctgctgttt atcatatata tacttaaaaa tacagcggct atacataccc
     4561 acgccagtac agatatgaat aatataaatg aggcggtctt catatacccg tcaagcttac
     4621 ctgctatctc gctgtaattc tggtcaaagt aatagaacat gcctccgtag cctaaagctt
     4681 ccatctcttt ttcaaactcc tctgttttcc cgttttcaat aaccaccgag aaagaaccgg
     4741 gtatgctcgt tctctccttt ttaacagacc gtacctttcc gtctcccgct cttctcaatt
     4801 cggctacaat cggcttggta ttaaaatcac cctcaatgga ttttgaggga gctatcagca
     4861 cgttattaga aagtgtcagt tctcctctgt ctcccgatcc tttatttgtg tatgctccga
     4921 taatctcata ctctacttcg gttatgaaat cctgccctct atatcccaaa ggagcaaagt
     4981 aatcctcatt actttccggc tgcccggtat cctttctgat tatcatctta ctatctactg
     5041 taaagtcact ttcataaacg gaaagcttta tcctatcgcc tactcttaaa tcatttgtaa
     5101 ctgatacagg cgcacttatt acacagactt tagcaccttg ttcatactcg tctttactta
     5161 tcgccctgcc tctgtctata tatgaatcgc cggcattgaa cggatacata ctgtacatat
     5221 cgtcggtaag caatatctcc atagaatgct tggttatctc acaattgtct accaatctgt
     5281 tccatttctg atcctcggag tctaaaagag attgagcgtc ggtactcaga gcctgaaacg
     5341 ggtgtgtgga atcatcgaaa acacgccata ctttatcacg atctatttcc ggccgttctt
     5401 ttttctcttc ttccgaaaga gtatagagat ctccgtatac acccacatcc ggaccaagag
     5461 ttccgttgta atccagtcgt cctgaaaaca taccttcttc gtaatcccaa ccgaacatgt
     5521 aagcatgtat tatgtacttt tctcctactt tgaacggaaa ccgtaagtcg tttgttagta
     5581 gtgtaccccc tattaatatg taatcactat tatatctcgg acccggcttt tcaacaaaaa
     5641 ggggaaatat attcaaatca tttttgtcaa tcttgaaaac cgccctataa tttgagtctc
     5701 gccaaaaacc tatgcgctca catactcccg taattatggc atacgaatac ggataaaatc
     5761 ttgttcttgt accgctgatg ataaagctcg ttatatccgg actatatccc atcagatatt
     5821 ttcgcctgtc ggtatatttt gtatattgcg agttctctgc ggcttgtttt actttaccaa
     5881 gcaactcgta atcatattta ggctcctcgg aagatacata tatctgttcc gtaaactcca
     5941 actctcttac aatacctatc gtggtgaatg catcttttga atattt
//
LOCUS       DAOSOM010000071         5544 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_14794_length_5544_cov_4.626708, whole genome shotgun sequence.
ACCESSION   DAOSOM010000071 DAOSOM010000000
VERSION     DAOSOM010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5544
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14794_length_5544_cov_4.626708"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1118
                     /locus_tag="PLF14_09705"
     CDS             <1..1118
                     /locus_tag="PLF14_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III restriction endonuclease subunit R"
                     /protein_id="HOT71594.1"
                     /translation="SIDYLGQEASEVIYRQLVDNGYIDNTGRVQDKLKIDIRNNDIQI
                     SGEFESIKTDIITIVSKVAGSLNIKNNSDKKQVRLNKQVYLDPEFKELWDKIKYKTTY
                     SVEFDSKKLIEVCCEAIKNNISVSSAKLIYTKAELAISAAGVTSEESSRYAVALHNLQ
                     ENLPDIIAYLQNETYLTRKTIVEILIKSDTLHLFKKNPQRYMEQVVQIITAKMRLMIV
                     DGIKYTKIGDEEYYAQELFETEELTGYLSKNMIESKKSVYEYVIYDSANEEDFAINFE
                     NNNSIKLYAKLPRWFTIATPLGSYNPDWVVLIEVDGKDKLYFVVETKANIQAESIRPV
                     ERAKIKCGKKHFEALGNEVIFKDIDNYKDFIEEYVVV"
     gene            complement(1263..2432)
                     /gene="nagA"
                     /locus_tag="PLF14_09710"
     CDS             complement(1263..2432)
                     /gene="nagA"
                     /locus_tag="PLF14_09710"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HOT71595.1"
                     /translation="MRTIIYNGKLILKDTITEQGHVEITDGVITNVSQHNPNHDLLSH
                     ADNIIDAKGMHISPGFIDIHTHGGGGYDFGVNSTDSYIVPCEIHARHGTTSLYPTISS
                     ATFETMEEAVVLYKEAKYINYSGATMRGLHFEGPYFSPAQKGAQSEEHLRTPKPFEYL
                     KILNMTDDIKRWSSACELDGMQEFTYELKKRGILPAIGHCDATYEEVKQAQEWGFSLI
                     THLYSCCSTITRDKGYRILGVTEAAYLIDDMDVEIICDGHHLPDGLIQMVYRFIGPDR
                     TAIITDCLSYAGLDAPDGTLLGDRFVVEGGVAKLLDRSAFAGSIATFDMLIRTALKAG
                     LPLKDIIKMSSVTPARIMGIDKQTGSLEKGKEADVIVFDNDINMHAVFVKGKRVL"
     gene            2523..3281
                     /locus_tag="PLF14_09715"
     CDS             2523..3281
                     /locus_tag="PLF14_09715"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOT71596.1"
                     /translation="MKLAAQLYTLREYTQTETDYYNTCVKVKNMGYSSVQLSGAGPME
                     AKTVKSISDELNLPVTATHIPHTMFVNELDKVIQNHKIMGCKYVGLGYMPKEYWESVS
                     TLNEFITTYSKVAKALDKEGLRFVYHNHHFEFFKFKGKTMMDIILENTPSCFFFLPDT
                     YWIQAAGANPVYWIKKLKGRGDYIHFKDMGIGSDYKTTFVPVGQGNLDWQSIIEACRY
                     AEVKYIAVEQDTCEGSPFDALKASYDYLSSMGLK"
     gene            3292..4455
                     /locus_tag="PLF14_09720"
     CDS             3292..4455
                     /locus_tag="PLF14_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT71597.1"
                     /translation="MSEVRFGIIGCGNIAVTHLGFFDRNEIPNARVTAIFDIDKSRTD
                     AVREKFKDKIAVFDNEEEFFSSKLFDAILICVPHYHHPHYAIKGFEHNLHVLCEKPAG
                     VYTKQVREMNEAYEHHKHLVFAMMYNQRTNPLYRKVRNMVQSGEYGEIRRCVWIITDW
                     FRTQRYYDSGGWRATWAYEGGGVLLNQDPHQLDLWQWICGMPVRVRAFLGFGKFHDIE
                     VEDDVTAYVEYPNGATGVFITSTGEAPGTNRFEISLDRGKIVIENNKICAYKLKEDLS
                     RFIKQSDKPFTAPEYETIEYDIDEPNLQHKGILINFVEAILNNAPLIAPGPEGILGLT
                     ISNAMHLSQWTDSWVDIPFDEELFLKHLNKRIEQSTFTKKTVKATETDMSDSF"
     gene            4578..4907
                     /locus_tag="PLF14_09725"
     CDS             4578..4907
                     /locus_tag="PLF14_09725"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT71598.1"
                     /translation="MIDYKYIGQRIKEKRKSLNLTQHQLSQISQISAAYISRIEAGLQ
                     NMTLDTLEKVARSIDEDAIYLITGVRYEEDNCIKHICNNTSTEFVYLLEQALLELKRQ
                     GILNIQL"
     gene            complement(4890..>5544)
                     /locus_tag="PLF14_09730"
     CDS             complement(4890..>5544)
                     /locus_tag="PLF14_09730"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOT71599.1"
                     /translation="RKSFFTFAIAGYTNAGKSTLLNTLTDSDVMAEDMLFATLDPSAR
                     SMKLKSNKDAMLIDTVGFISKLPVKLVEAFKSTLDEIRYADCILHVIDGASKDADMQM
                     NEVYSILKSIGAGHIPVIEVVNKTDLVKEPLLQATSYPRAYISCVTGEGMETLLDLMD
                     QAAKDKHVYMKLNVDYADGKLLSFIHSNAKILSKRDTGEDIEMEIEISAELVSKLDI"
BASE COUNT         1770 a         1057 c         1146 g         1571 t
ORIGIN      
        1 gtagcataga ttatttggga caagaggctt cggaagttat ctataggcag ttagttgata
       61 acggatatat agataatacc ggtagagttc aagacaagct taaaattgat ataagaaaca
      121 acgatattca gatatcagga gagtttgagt ctataaaaac tgatattata actattgtca
      181 gcaaggtggc aggttcactt aatatcaaga ataacagcga taaaaagcag gtccgactta
      241 ataaacaggt ttatttagat ccggagttta aagagttgtg ggataaaata aaatataaaa
      301 caacatattc tgttgagttt gatagtaaaa aacttattga ggtatgttgc gaagcaatta
      361 aaaataatat ttccgttagt tctgccaaat taatctacac aaaggcggag cttgctatca
      421 gtgctgccgg agtcacatct gaagaatcga gcagatatgc tgttgccttg cacaacttac
      481 aggaaaatct ccctgatatc attgcctacc tccaaaacga aacttatctt acaaggaaga
      541 ccatagtaga aatactcata aaaagtgata ccctacatct gttcaagaag aatccgcaaa
      601 ggtatatgga gcaggtagtg cagataataa ctgcgaaaat gcgacttatg atagttgatg
      661 gcatcaagta taccaaaatt ggtgatgaag aatattatgc tcaggaacta tttgaaactg
      721 aagaacttac cggatattta tcaaagaata tgattgaaag caagaaatct gtttatgaat
      781 atgttattta tgacagtgca aatgaagaag actttgcaat taactttgag aataataata
      841 gtataaaatt gtatgcaaag cttccaagat ggtttaccat agccacacca ttgggctcat
      901 acaatcctga ctgggttgta ttgattgagg tagacggtaa agacaaactt tactttgttg
      961 tggagacaaa agcaaatata caagctgaga gtataagacc ggtagaaaga gccaaaataa
     1021 agtgtggaaa gaaacatttt gaagcattgg gtaatgaggt tatattcaaa gatattgata
     1081 actataagga ttttatagag gagtatgtgg tcgtataaca ggagttttac ttgttatgaa
     1141 gcaactgaat gccagttatt tcttctaaaa gattgtttta aaagtattat cacaaaagtg
     1201 gcactgttag tgccatttag tttttagtca attccgactt atgtagagtt ttaggtgcaa
     1261 tattacaaca cacgcttgcc ctttacaaac accgcatgca tattaatatc attatcaaaa
     1321 acaataacgt ccgcttcctt gcctttttca agtgaaccgg tctgtttatc aatgcccatt
     1381 attcttgcag gtgttacgga agacatcttt ataatatcct tcagaggcaa ccccgccttc
     1441 aatgccgtcc gtataagcat atcgaatgtg gcaatggagc ccgcaaaagc agacctgtcc
     1501 aaaagcttgg caactccgcc ttccacaaca aatctgtcac ctaataaagt gccgtccggg
     1561 gcatccagtc ccgcatagga aaggcagtcg gtaataatag ccgttctgtc cggtcctata
     1621 aatctgtata ccatctgaat aagaccgtcg ggaagatgat ggccgtcaca gataatctcc
     1681 acatccatgt cgtctatcag ataagcggct tcggttacgc ctaaaatacg gtatccttta
     1741 tctcttgtta ttgtggaaca acaggaatac agatgggtta tgagggaaaa tccccattct
     1801 tgtgcttgct taacctcttc atatgtggca tcgcagtgcc ctattgcagg aagtattcct
     1861 ctttttttca attcatacgt aaattcttgc ataccgtcca actcacatgc cgaagaccac
     1921 ctctttatat catcggtcat gttaagtatt ttcagatatt caaaaggctt cggtgttctt
     1981 aaatgctctt cgctctgcgc acctttctgt gcaggcgaaa agtacggacc ttcaaagtgc
     2041 agccctctca ttgttgctcc cgaataattt atgtactttg cttctttgta taacacaaca
     2101 gcttcttcca tggtttcaaa agttgccgat gaaattgtgg gatacagact tgtggtaccg
     2161 tggcgtgcat gaatttcgca ggggacgata taggaatccg tgctgtttac tccgaaatca
     2221 taacctcccc cgccatgggt gtgtatgtcg ataaatcccg gagaaatatg catgcctttt
     2281 gcatctatga tattgtccgc atgtgataat aaatcatgat tggggttatg ttgtgataca
     2341 tttgtaataa ctccgtcggt tatttccaca tgtccttgct cggttattgt gtctttaagt
     2401 atgagttttc cgttataaat aattgtacgc ataaattttc cttccttccc ccattatatt
     2461 atttaaccgt tgaataaaca accttgtaag gtataattaa agtatcagaa ggagaaacaa
     2521 aaatgaaact tgcggcacag ttatatacac ttagggaata cacacagacc gaaacggatt
     2581 attacaacac atgtgttaag gtgaagaata tgggttattc ctccgtccag ctctccggag
     2641 caggtcccat ggaagcaaaa acggttaaaa gtatctcaga tgagttgaat ctgccggtta
     2701 cggcaacaca tataccccac acaatgtttg taaatgaatt ggataaggta atacaaaacc
     2761 ataagataat gggatgcaaa tatgtgggat tgggttacat gcctaaagag tattgggaga
     2821 gcgtaagcac attgaatgag tttataacca cctattcgaa agttgcaaaa gcactggaca
     2881 aagaaggctt acggttcgta taccacaatc atcatttcga gttcttcaag ttcaagggta
     2941 aaacaatgat ggatataata ttggaaaata ctccctcctg ttttttcttt ttgccggata
     3001 catactggat acaggctgcg ggagcaaatc cggtatactg gataaagaag ttaaaaggca
     3061 gaggcgacta tattcacttt aaagacatgg gcataggcag tgattataaa acaactttcg
     3121 tgccggtagg acagggaaat ctggactggc aaagcatcat agaggcatgc agatatgcag
     3181 aagtaaaata tatagcagtg gaacaggata catgtgaagg ttcgccgttt gatgcgctga
     3241 aagcttccta tgattatctg tcatcaatgg gattgaagta aggagattag catgagtgaa
     3301 gtacgattcg gcataatagg ttgcggtaac attgcagtta cgcatttagg tttttttgac
     3361 agaaacgaaa taccgaatgc ccgtgtaacc gctattttcg atatagataa atcaagaact
     3421 gatgcggtaa gagaaaaatt caaggataaa atagcggtct ttgacaatga agaagagttc
     3481 ttctcatcaa agctctttga tgccatactc atatgtgttc cgcattatca tcatccgcac
     3541 tatgccataa aaggatttga gcacaacctt catgtactgt gcgaaaaacc ggcgggagta
     3601 tatacaaaac aggtgcgtga aatgaatgag gcttacgaac atcataagca tcttgttttt
     3661 gccatgatgt acaaccagag aaccaatccg ctgtacagaa aggtacgtaa tatggttcaa
     3721 agcggcgaat acggcgagat caggcgttgt gtctggataa taaccgactg gttccgtact
     3781 caaagatatt acgattcggg aggttggaga gctacatggg cttatgaagg aggcggagtg
     3841 ctgttaaatc aggaccccca ccagctggat ttgtggcaat ggatatgcgg tatgcctgta
     3901 agggtacgtg cattcttagg tttcggaaag tttcatgata ttgaagtgga agacgatgta
     3961 accgcttatg tggaatatcc caacggagca acgggagtat tcatcacttc cacaggtgaa
     4021 gcccccggca caaacaggtt tgaaatttct ttagacagag gcaagatagt aatagaaaac
     4081 aacaaaatat gcgcatacaa gctgaaagaa gacctgagca ggtttattaa gcagtcggat
     4141 aagcctttta ctgcaccgga atatgaaact attgaatatg atatagatga gcccaatctg
     4201 cagcataaag gaatactgat aaacttcgtg gaggcgatac tgaataatgc acctttaatc
     4261 gcacccggcc ccgaaggaat attgggattg acaatatcca acgccatgca cctgtcacaa
     4321 tggaccgatt cgtgggtgga tattcctttt gacgaagagc ttttcctgaa acacttgaac
     4381 aagagaatcg agcaatcgac ttttactaaa aagactgtaa aagcaactga aacggatatg
     4441 tccgattctt tctgagcggt cgttttttaa tatcaaatat aaattttaaa agtaatacct
     4501 ttttgtctat tcaatgatac aaaaacgata tgtatcatat taaatagtgt taactatatt
     4561 atgaatgggg caagtacatg attgattata aatacatcgg acagagaata aaggaaaaac
     4621 gtaaatcgct caatcttaca caacatcagt tatcgcagat ctcgcagata tccgccgcat
     4681 atataagcag aatagaagcg ggattacaga atatgacttt agacacactg gaaaaagtcg
     4741 cccggtccat cgacgaagat gccatctatc tcattaccgg tgttagatac gaagaagata
     4801 actgcataaa acacatatgc aataacactt ccaccgaatt cgtatatctt ttagaacagg
     4861 cattgttaga gttaaagcgc cagggcatac taaatatcca gctttgagac cagttcggcc
     4921 gatatttcaa tctccatctc aatatcctca cccgtatctc tctttgacag aatcttcgca
     4981 ttggaatgta taaatgaaag aagctttccg tcggcataat ccacattcag tttcatatat
     5041 acatgcttgt cttttgccgc ctgatccatt aaatccagca gtgtttccat tccctctccc
     5101 gtcacacagg aaatatacgc tctgggatag gaagtagctt gcagaagcgg ttcctttact
     5161 aaatcggtct tgtttacaac ttcaataacc ggtatatgac cggctcctat gcttttaagt
     5221 atggagtata cctcattcat ctgcatgtcg gcgtctttgc ttgctccgtc tataacatgc
     5281 agtatacagt ctgcatatcg tatttcatcc aacgtggact taaaggcttc caccagttta
     5341 acgggcagtt tggatatgaa tcctaccgta tctatcagca ttgcatcttt attggacttt
     5401 aacttcatgc ttctggccga aggatccaat gtggcaaaca gcatatcttc cgccattaca
     5461 tcgctgtctg taagtgtatt taacaaagtg gatttgccgg cattggtata ccccgcaata
     5521 gcaaacgtga aaaagctctt tctg
//
LOCUS       DAOSOM010000072         5509 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_14897_length_5509_cov_4.814081, whole genome shotgun sequence.
ACCESSION   DAOSOM010000072 DAOSOM010000000
VERSION     DAOSOM010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14897_length_5509_cov_4.814081"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1196)
                     /locus_tag="PLF14_09735"
     CDS             complement(<1..1196)
                     /locus_tag="PLF14_09735"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOT71600.1"
                     /translation="MLLKDLLKVTGCIRISGSKNADITDISFDSRKTKPGHLFIAIEG
                     VDDDGHKYIGNAVKNGAVAVVLTKDFEHYVNNYPDVSVIETDDARIFLAKASAYYFNY
                     PANALNLIGITGTKGKSTISYMVKNIFDKAGKECAVIGTVGAVYKDKETAISQTTPSA
                     YELNDIFSKLNEESVNNCVMEVSSIALKQNRVYGLRYKIGMYTNLTQAHITDREHPDF
                     SDYYNSKKRIFSASDVMIVNYDDLHVRKATEEYKSSEGYNKDRQRIYTIGIEETADIT
                     ADNILYRNDGSSFIYRGLGMELEIGLDMPGRFNIYNSLFAVTACLLNDIDVKYIKQGL
                     SDVVVKGRCEKVDTGKDFTVMIDYAHTPDSLEKLLIAVRETRTSGRIICLFGCGGDRD
                     KSMRPM"
     gene            complement(1217..3430)
                     /locus_tag="PLF14_09740"
     CDS             complement(1217..3430)
                     /locus_tag="PLF14_09740"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOT71601.1"
                     /translation="MDKKKEKEKKPENIENTSNNTLTRKRLLILMAVFILIAMIIAGY
                     TGYIQFVWANELRVKAYEQQNRDRTIKAERGTIYDSSGNVLAISVSVDTISVSPIEVR
                     KNKNITVEEVAQELSSVLELDYENVLDKLNMDNHYQLIASKVERSTSDLLKAFIKEHK
                     VSGIYIHQDSKRYYPFGSLAAHVIGFTGDDNQGLYGLELSLDNYLRGTDGKILTELDV
                     AGTELPFAFESRIEAVDGYDVITTLNTDIQKIAENVVKQAIEDNKAEGGAVCIVSDCK
                     TSEILAMVSEPGMDLNNPRALPEGYTIDGWTGLRKSDVDLLYSTVWRNKAIMDTYEPG
                     STFKSVTTAMALEEDIVQQDTMVDDFPVTVYGATIHCWAKQYPHGKETFLQAVYNSCN
                     PVFVKVSHLIGIKRFYEYIESFGFRDPSGIELMGEGQSIFHSEPTKLDMSVASFGQRF
                     QITPIQMVAAYNAIANGGYLIKPGLTDAIADKQGNIIYDFEPQVVRQVISEETSDEMR
                     KILQGVVSEGGGKNAYVDGYRIAGKTGTSETTVEGVYVSSFIGFAPADNPVITVLLIV
                     FNPQGDSYYGSQVAAPYAKLIFEQALEELSVEKQGTAGKEKTPVYVPNLNGYNVEKAA
                     KKIKSYGLNYEIEGSTAGEDVTIMYQFPESGTVIAKDGVIILYTYVPDNKMTVKMPYL
                     VGKDKSYVVKALKDLGLNINSDGDGECISVQYPAGTYVEKGTIVNITFRFTDYPD"
     gene            complement(3443..3865)
                     /locus_tag="PLF14_09745"
     CDS             complement(3443..3865)
                     /locus_tag="PLF14_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71602.1"
                     /translation="MEIERNDEKRIFNRFNSVVLLVLVFIALFIIIYRYGEMINLNYD
                     ITSLNNQLREKTAVNSAIYADLDRQYNIDDIKNIAETKLDMNKPRADQRILIHPDMSD
                     RYQSSEKEDFYSYGRAVWEKIKHTAENISDIFIGSKKD"
     gene            complement(4004..4948)
                     /gene="rsmH"
                     /locus_tag="PLF14_09750"
     CDS             complement(4004..4948)
                     /gene="rsmH"
                     /locus_tag="PLF14_09750"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOT71603.1"
                     /translation="MKEYSHESVMADECIEYMNIDKRGTYIDCTAGGGGHSVRIYERL
                     GDEGLLIACDQDIEACKVTKDKLAKYKRKTKFIVLQENFVNIDKVIEQYCPEGADGIL
                     IDLGVSSYQLDNRERGFSYHNDGPLDMRMDQTKEKTAEYVVNDYTKDELVRIFYEYGE
                     EKHSRRIAEAIVHKRKENRITTTGMLTDIIKQAYPPKERFAGKHPARKTFQAIRIEVN
                     DELNVLKEVLPKAAFALKKNGRLAVITFHSLEDRIVKDTFNELVNPCTCPKSFPVCVC
                     NKKAQHKLVLKKPMVPKTEETESNYRSRSSKLRVLERT"
     gene            complement(4948..5412)
                     /gene="mraZ"
                     /locus_tag="PLF14_09755"
     CDS             complement(4948..5412)
                     /gene="mraZ"
                     /locus_tag="PLF14_09755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOT71604.1"
                     /translation="MYGEFTHRVDDKGRVILPVSMREVLGDKFVIAKSVDSTLKILYL
                     YTRKDWENIIEAAAKIAARDRHKAVYVRLLSASGKDTELDSQNRVLIPIELRNYANLG
                     KEAVLLGYNNRVEIWNKESYHAAHPDDSEISEQVALENADNVAAILEEIPLA"
BASE COUNT         1389 a         1333 c          949 g         1838 t
ORIGIN      
        1 atcatgggtc tcatggactt atccctgtca ccgccgcatc cgaacaggca tattatccga
       61 ccggatgtcc tggtttcccg caccgcaatg agcagttttt ccagactgtc aggcgtgtga
      121 gcataatcaa tcattacggt gaaatcttta cccgtatcga ctttttcgca tcttcctttg
      181 acaactacat cggaaagccc ttgtttgata tattttacat caatatcgtt gagaagacat
      241 gcggtaacgg caaacaggct gttatatata ttaaatcttc ccggcatatc caagcctatt
      301 tccaactcca tacctaaacc tcggtaaata aaactgctgc cgtcattccg gtacagtata
      361 ttatccgcag ttatatccgc agtttcctcg atgcctatag tatatatcct ctgcctatcc
      421 ttgttataac cttcactgct tttgtactct tcggtagcct ttctgacatg caaatcgtcg
      481 taatttacaa tcattacgtc acttgccgaa aatatccgtt tcttggagtt atagtagtcg
      541 gaaaaatccg gatgttccct gtcggtaata tgtgcctgag taagattggt atacatgcct
      601 atcttatacc gtaatccgta taccctgttt tgtttcaacg ctatggagga aacttccatt
      661 acacaattgt taacactttc ttcatttaac ttactgaaaa tatcatttaa ttcatatgcc
      721 gaaggcgttg tctgcgatat ggctgtttcc ttgtctttat agaccgctcc cacggttcct
      781 ataaccgcac attctttacc ggctttgtca aaaatgttct taaccatgta tgatatggtg
      841 gatttgccct tggttccggt tattccgata agattgagag catttgcggg ataattgaag
      901 taatatgcag aagcttttgc taaaaatatt ctggcatcat cagtctcaat gaccgaaaca
      961 tcgggatagt tatttacata atgttcaaaa tctttagtca aaacaacagc aaccgcaccg
     1021 tttttaacgg cattacctat atatttatgc ccgtcatcat ctaccccttc tatggctata
     1081 aacagatgac ctggctttgt ttttctggaa tcaaaggata tatcggtaat atccgcattt
     1141 tttgagcctg aaatccttat acaacccgtt actttcaata aatctttcag aagcattcta
     1201 cctcaatcat cctttcttaa tccgggtaat cggtaaaccg gaaagttata ttcacaatag
     1261 tgcccttttc cacatatgtt ccggcaggat actgaacgct tatacactct ccgtctccgt
     1321 cggaattgat gttcaatccc aaatccttca atgctttaac aacatacgat ttatccttac
     1381 ctaccagata cggcatcttg accgtcatct tgttatcggg aacgtaggta tataatatta
     1441 taaccccgtc tttggctata acggtaccgc tttcgggaaa ttgatacata atagtgacat
     1501 cttcacctgc cgtactgcct tcaatctcat aattcaagcc gtaactcttt attttcttag
     1561 cggctttttc cacattatac ccgtttaaat tgggaacgta taccggagtt ttctccttac
     1621 ccgccgttcc ttgcttttct acggacaatt cttccaatgc ctgctcgaag ataagttttg
     1681 catagggagc cgcaacctga ctgccgtagt aggaatcacc ctgtgggttg aatacaataa
     1741 gcaacaccgt tataacagga ttatccgcag gagcaaatcc gataaaagaa gacacataca
     1801 cgccctcaac cgtcgtttca ctggttcccg tcttaccggc tatcctgtaa ccgtcaacat
     1861 aagcattctt tcctccgccc tccgatacca ctccttgcag aatctttctc atttcatcgg
     1921 atgtttcttc ggatatgacc tgtcgcacta cctgtggctc aaagtcgtaa attatattcc
     1981 cctgcttgtc cgctatagca tcggtaagac cgggtttaat aagataaccg ccgtttgcaa
     2041 tcgcgttata tgccgctacc atctgtatgg gtgttatctg aaatcgctga ccgaaggaag
     2101 caaccgacat atccagtttt gtgggctcgc tgtgaaatat gctctgccct tctcccataa
     2161 gctctattcc cgaaggatct ctgaatccga atgattctat atactcataa aacctcttta
     2221 tacctataag gtgagatact tttacaaaaa ccggattgca ggaattgtat acggcttgta
     2281 agaatgtttc tttgccgtga ggatattgct ttgcccaaca gtgaattgtg gctccgtaaa
     2341 ccgtaacggg aaaatcatcc accatcgtat cctgttgaac tatatcctct tccagtgcca
     2401 ttgcagtagt tacggacttg aatgtggaac cgggctcata tgtgtccatg atagccttat
     2461 tgcgccatac ggtgctgtac aacaggtcta catcactttt cctcaaaccg gtccatccgt
     2521 ctatcgtgta gccttcgggc agtgctctgg ggttattcag atccattccc ggttcggaga
     2581 ccatggccag tatttccgat gttttacagt cggaaactat acataccgca ccgccttcgg
     2641 ctttattgtc ttctatagcc tgtttaacca cattctcggc aattttctga atatcggtat
     2701 ttaaggtggt tattacatca taaccgtcta ccgcttctat tctggattca aatgcaaaag
     2761 gaagctcggt tccggcaaca tccagttcgg taagtatttt accgtcggtt cctctaagat
     2821 agttgtccaa agataactcc agaccgtata atccctgatt gtcatcaccg gtaaacccga
     2881 tgacatgagc ggcaagagaa ccgaacggat aatatctttt actgtcctga tgtatgtata
     2941 ttcccgaaac cttatgctct tttataaagg cttttaacaa atcggaagtg ctccgctcca
     3001 ccttggacgc tataagctga taatggttgt ccatattaag cttatccagc acattttcat
     3061 aatccaactc cagaaccgaa ctgagctcct gtgcaacttc ctccaccgtt atattcttat
     3121 tcttacgtac ttcaataggg cttacggaaa ttgtatccac gcttacggat attgccagca
     3181 cattacccga actgtcataa atagtgcctc tttcggcttt tatggttctg tcccggtttt
     3241 gctgctcgta tgccttgacc cgcaactcat tggcccacac gaattgtata tatcccgtat
     3301 agcccgcgat aatcattgct attaaaataa agaccgccat aagtatcaac agcctttttc
     3361 ttgtcaatgt attgtttgat gtattttcga tattctcagg tttcttttct ttttcttttt
     3421 ttttatccat tcatttaccg attcaatcct tcttcgaacc tatgaaaata tccgatatgt
     3481 tttctgcagt atgttttatc ttttcccaaa ccgcacgtcc ataggaatag aagtcttcct
     3541 tttccgaaga ctgatatcgg tccgacatat cgggatgaat aagtatccgc tgatcggctc
     3601 tcggcttatt catgtccagt ttggtttccg caatattctt tatatcatct atattgtatt
     3661 gtctgtcgag atccgcatag atagcggaat tgactgcagt cttttcacgt aattgattgt
     3721 ttaatgacgt aatatcatag ttcagattaa tcatttcacc gtatcggtaa atgattataa
     3781 acagagcaat aaataccagt acaagcagta ctaccgaatt aaatctgtta aatattctct
     3841 tttcatcatt tctctcaatt tccatgttca tccaataatt tctcgggtta aaacccgccg
     3901 gttgcaaaag ggggaatttt tacaaccggc ttccgtcttt agtgttttta aatttcttga
     3961 gtgcttttac gcttctttag cgttttatct ttagtgtgta tctttaagtt ctctctaaga
     4021 ctcttagctt ggaacttctg gatctgtagt tgctttccgt ctcttccgtt ttcggaacca
     4081 tcggtttctt taataccagc ttatgttgtg ctttcttatt gcagacacat accggaaagg
     4141 acttggggca tgtgcagggg ttaaccaatt cattgaaagt atcttttact atcctgtctt
     4201 ccaacgaatg aaatgtgata accgcaagcc tgccgttttt cttcagtgca aatgcagctt
     4261 tgggaagcac ttccttcaga acattcagtt catcgttaac ttcaattctg atggcctgaa
     4321 atgtttttct tgcaggatgc ttaccggcga atcgctcttt cggcggatat gcttgtttaa
     4381 tgatatccgt cagcattccg gtagtggtta tcctgttttc ttttcttttg tgtactatag
     4441 cttccgctat tcttcttgag tgcttttctt ctccgtattc ataaaaaata cggacaagtt
     4501 catctttagt gtaatcatta accacatact ctgcggtttt ttcttttgtc tgatccattc
     4561 tcatatccaa cggtccgtcg ttatgataag agaatcctct ttcccggttg tccagttgat
     4621 acgatgaaac tcccaaatca atcagtattc catcggctcc ttcaggacaa tattgctcta
     4681 ttactttgtc aatgttcacg aaattctcct gcagtactat gaatttcgtt tttcttttgt
     4741 actttgctaa tttatctttt gtaaccttac aggcttcgat gtcctgatca caagctatca
     4801 gtaatccttc atcgcctaat ctttcgtata tccttaccga atgaccgcca cctccggccg
     4861 tgcaatcaat gtaagttcct cttttgtcaa tattcatata ttcaatacat tcatcggcca
     4921 ttaccgactc atgtgaatat tctttcatta cgctaacggt atttcttcca ggatagccgc
     4981 aacattatcg gcattctcca gagcaacttg ttcggatatc tccgaatcat cgggatgtgc
     5041 ggcatggtag gattccttat tccatatctc caccctgttg ttatacccca acaataccgc
     5101 ctctttacct aaattggcat aatttctaag ctcaatggga atcaacactc tgttctggct
     5161 gtccagttca gtatccttac cgcttgccga caataaacgg acataaacgg ctttgtgcct
     5221 gtccctggca gcaatctttg ctgcagcctc gataatattt tcccagtctt ttctcgtgta
     5281 taaatatagg attttcaatg tgctgtctac gctttttgca ataacgaact tatctccgag
     5341 aacctcacgc atggaaacag gaagaataac cctcccctta tcatccactc tatgcgtaaa
     5401 ttctccatac attgccatag tttttcaaca ctttccgcca tttcggcacc acttccacac
     5461 cacttgcccc cattatatac atcaaaaaca gatgtgtcaa caccttttt
//
LOCUS       DAOSOM010000073         5350 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_15439_length_5350_cov_4.775071, whole genome shotgun sequence.
ACCESSION   DAOSOM010000073 DAOSOM010000000
VERSION     DAOSOM010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15439_length_5350_cov_4.775071"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="PLF14_09760"
     CDS             <1..619
                     /locus_tag="PLF14_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71605.1"
                     /translation="GLYWGTKPVLSEDGRFLLYLTNRRNNGKTNDIRLYDFESKEDVL
                     LAKDAYYNRAYISDTTVFYTCNDMLCRIEIPSKVKATVSVSVSPNGHFCYPYYIYTAD
                     YYQKYEILNILSLNVEKGSLGIEKSALKIVPMKTDTDYIAAVLLLNRNNVTLSFIDIK
                     TNKLLKTFVLSDSFRVVYTQWIDDNTFLVSGYEGGDNEKTYLLSY"
     gene            591..1592
                     /locus_tag="PLF14_09765"
     CDS             591..1592
                     /locus_tag="PLF14_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71606.1"
                     /translation="MRKHTYFRTKYITVVLLILLFPLLTVLSASDYDRAFVQKTADNL
                     SIQALSPYKNSQGIEYWPFCTSKNNQPRYVSGTNPHQGTDLTINVGEYIYPIYDGEIV
                     YIKKDTSAQLGHIVVKSDIGYEEPVYIEYLHVIPVQGLDENDYVYTSIPIATIDEYNR
                     YDSHLHIGRVNEDRTLHYQLYDLFSDVLRWNNGSDLDVFSYPLFNDETNIFSVTAYVS
                     SDTENLDYYGGYGRFSLKYITFHYSVNNSTWKSFNIAEHDNDFRYSFNIKDMTGSKSK
                     DIVRYYITATRDNQSNTDTTFKDAPYTVAYYPAYYAHPSPALTKEQADKIALSLTIK"
     gene            complement(1585..3327)
                     /locus_tag="PLF14_09770"
     CDS             complement(1585..3327)
                     /locus_tag="PLF14_09770"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT71607.1"
                     /translation="MLKDMKFIFRLMGKNKGRYSVLLAAWSIIGSLVDMTFSYALKLL
                     TDFFVYKEKENVLLLILVFSASIVSSILFCVFFHLHNKVKEDILRDIRMMTFKEVQKL
                     PSSYFKATHSGDLVSRFNADTQSVITVFVEFEHIFESAIVLLMRIPFALLLDLRFGAI
                     LLGFSVFTGVLNVLFIKPLREKHKNILKDKASLSKQASEVVTGFTVARMFSLSTFLRS
                     RFEKSVQDSFDSGWKAAKTYTLQRGLNTIIWNFSHYLSTILGALFIINGTLEAGNYMA
                     ISTSTGIGWSVSILITALPNLQKAFAGSNRLKELYDEPKEPELYDIEGTGTDMGIEMS
                     KVDFAYEIRDNEEKEENNFRISGLSLDIEQGKTLALVGDSGGGKSTIAKLILGLFKID
                     EGKMQINGKAYKDYTLEQLRESIGYVPQDAYIFNGTITENIKYGKPDATEEEIVNAAM
                     LANAHDFILAQPNGYETYVGERGIRLSGGQRQRIAIARAIIKNPSILLLDEATSSLDS
                     ESEKMIQDALDRLIKTKTSLVIAHRLSTIMNADRICYIKDGYVAEQGTHAELLAKKGL
                     YHELYYREFAKETI"
     gene            complement(3320..5092)
                     /locus_tag="PLF14_09775"
     CDS             complement(3320..5092)
                     /locus_tag="PLF14_09775"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT71608.1"
                     /translation="MAKKDKETPEKKEKTDVKYVIKTMVKLYKESAEHKKWAFGRWFG
                     GVVSGILAVVIFFVTGSVLDVALSGDYNRLYTLIYALSAIFVIRFILQFVNPLIASQY
                     ELRTHKTLAMNVYDKIDKLEMGYYENVHSADTISTLVDDVEKIKTFMGNTVAGFLSYN
                     PVSFILSMFFLVRINWKLTVFSLTVIPLIMYLIDKASKPLREVKNKIQEYTAVLNSYI
                     RDFVEGNDIYKSFNMHKSHTAKVQQSCQDIAEESYKDSFVMTKNRALTVLMMLIPQGV
                     CMFASMFFIARNELTIGQYVIFSNLLWPLIQTFRTTGMGWADIVGYTGTAERYFKFLE
                     TKEERSDGEDFGTQDSDIIVEFKNVGFSYHEDIPLLKDVSFKLHKNSRLALCGVSGSG
                     KSTIFKLICGYYENFTGEIYVFGHSIRSWNLKALRKYMTVVTQDVYLFDDDIINNIKM
                     GNLEATDEMVEDASKKAYIDKLVKDVGERGAKVSGGQKQRIAVARALLKDAPLIMLDE
                     PTSALDTKSEYYVEKSIENLKKGRSVLVIAHRLTTIIDSDCIILLEDGTVKEQGTHEE
                     LIRKKKRYAELYERQLVEEEAHNA"
     gene            complement(5101..>5350)
                     /locus_tag="PLF14_09780"
     CDS             complement(5101..>5350)
                     /locus_tag="PLF14_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71609.1"
                     /translation="GETTNGSTTVNINYNFGAVFECSAGKHGSIRLGSNLKAQSVERN
                     MQGRIIYAKGESRYAYKSDIIAAAEIKTDHGQIKMDFV"
BASE COUNT         1586 a         1168 c          906 g         1690 t
ORIGIN      
        1 cggtctttac tggggtacaa agccggtatt gtcggaagac ggacgctttc ttctttatct
       61 gaccaacaga agaaacaacg gaaaaacaaa tgatatccgg ttatatgact ttgaaagcaa
      121 agaagatgtc ctactggcaa aggacgctta ttacaaccgg gcatacatat ccgataccac
      181 tgttttttac acttgtaatg acatgttgtg cagaatagag ataccatcca aggtaaaagc
      241 gaccgtgtcg gtttcggtat ctcctaacgg tcatttttgc tatccctact atatttacac
      301 tgccgattac tatcaaaaat atgagatttt gaacatatta tcactgaatg tggaaaaagg
      361 atctctcggg atagaaaaat cggcattgaa gattgtgccg atgaaaaccg ataccgatta
      421 cattgcagcc gttcttttac tcaaccgcaa caatgtaact ttatccttta ttgatataaa
      481 gacaaacaaa ttacttaaaa cctttgtatt aagcgactca ttcagagtgg tgtatactca
      541 atggattgat gataatacgt ttcttgtaag cggatatgag ggaggagata atgagaaaac
      601 atacttactt tcgtactaaa tacataaccg tagtccttct tatactgctc tttcccctgc
      661 ttactgtttt atcggcttcc gactatgaca gagcgtttgt acagaaaacc gccgacaacc
      721 tgtcaataca ggcactctct ccgtacaaga actcccaggg catcgaatac tggccttttt
      781 gcaccagcaa aaataaccag ccgagatatg tatcgggtac aaatcctcat cagggtaccg
      841 atttaacaat taatgtggga gaatacatat accctatata tgacggtgaa atagtttaca
      901 ttaaaaaaga cacatcggca cagttgggtc atattgtggt taaatcggat ataggatatg
      961 aagagcctgt atatatagaa taccttcatg tgatacccgt ccaaggtttg gatgaaaacg
     1021 attatgtcta tacttccatt cccattgcca caattgatga atataacagg tatgattcac
     1081 atcttcacat aggaagagtg aatgaagaca gaacattaca ttatcaactc tatgatcttt
     1141 tttccgatgt tttaagatgg aacaacggct ccgatttgga tgtgttcagt tatcctcttt
     1201 tcaatgatga aaccaatatc ttttccgtca ccgcttatgt atcgtccgat acggaaaacc
     1261 ttgattatta cggcggttac ggacgtttct ccctgaaata tataactttt cattattcgg
     1321 taaataacag cacctggaaa agttttaaca tagcagagca tgataatgac ttcaggtatt
     1381 cattcaacat aaaagatatg acaggttcta aaagtaagga tattgtaagg tactatataa
     1441 ccgcaacaag ggataatcag agtaataccg ataccacatt caaagatgca ccgtatacgg
     1501 tagcatacta ccccgcatac tatgcacatc cgtcgcctgc ccttacaaag gagcaggcgg
     1561 ataaaattgc tttatctcta acaattaaat agtttctttg gcaaactctc tgtaatacag
     1621 ttcgtgatac agaccttttt tagccaacag ctccgcatga gtaccctgtt ccgctacata
     1681 accgtctttt atataacata tacggtcggc attcatgatg gtggaaagcc tatgcgctat
     1741 tacaagtgat gttttggttt tgataagtct gtccagcgca tcctgaatca ttttttccga
     1801 ttcgctgtcc aaagaggatg tagcttcgtc aagaagtaaa atggaaggat ttttaattat
     1861 agcccttgca atggcaatcc tttgcctttg tccgcccgat aaccttattc ccctctctcc
     1921 gacataagtt tcatatccgt tcggttgagc aagtataaag tcatgagcat ttgccagcat
     1981 agccgcatta acaatctctt cttcagtagc atcaggttta ccgtatttga tattctcggt
     2041 tatggtaccg ttgaagatat atgcgtcctg cggaacatac cctatgctct ctctcaactg
     2101 ctctaaagtg taatctttat aggctttacc gttaatctgc atcttacctt catcaatctt
     2161 aaaaagacct aagataagtt ttgcaatagt actcttgccg cctccgctgt cacctaccaa
     2221 tgccagtgtc ttgccctgct caatatccag acttaaaccg gatatgcgga agttattttc
     2281 ctctttctct tcattatctc taatctcata cgcaaaatcc acttttgaca tctctatacc
     2341 catatcggta cccgtacctt cgatgtcata taattcaggc tctttaggtt catcatacaa
     2401 ctctttcaat ctgttggagc ctgcaaaagc tttttgtaaa ttgggtaatg cagttataag
     2461 aatggatacc gaccagccaa ttccggtact ggtggatata gccatataat ttcccgcttc
     2521 cagagtaccg ttaatgatga ataaagcgcc taatatagtg cttaaatagt gcgaaaagtt
     2581 ccatatgatg gtattcagcc ctctttgcag agtataggtt tttgccgcct tccatccgct
     2641 gtcaaaactg tcctgaacac tcttttcaaa acgtgaacga agaaaagtac tcaaggaaaa
     2701 cattcttgct accgtaaagc cggtaaccac ttcggaagcc tgtttactta atgacgcttt
     2761 gtcttttaaa atatttttat gcttttctct taacggttta ataaacagaa cattcaaaac
     2821 accggtaaaa acggagaagc ctaagagaat tgctccgaac cgtaaatcca acagcagcgc
     2881 aaaaggtatt ctcattaata gaacaatggc agactcaaat atatgctcga attcgacaaa
     2941 taccgttatt acactctggg tatcggcatt aaatctggaa acaagatctc cgctgtgagt
     3001 agccttaaag taggaagaag gtagcttctg aacctctttg aatgtcatca tccttatatc
     3061 ccgcaatata tcttccttta ctttattgtg cagatggaag aatacacaaa agagtatgct
     3121 gctgacaata cttgcagaaa agaccagtat aagcaataat acattttctt tttctttata
     3181 cacgaagaaa tccgtaagca gcttaagcgc ataagagaag gtcatatcca caagactgcc
     3241 tattatactc catgccgcta acaaaacaga atatctgcct ttatttttcc ccatcaaacg
     3301 gaaaatgaac ttcatatcct taagcattgt gtgcctcctc ttctaccaac tgtctttcgt
     3361 ataattccgc atatcgcttt ttcttcctga taagttcctc atgggttccc tgttctttaa
     3421 cggtaccgtc ttcaagaaga ataatgcaat cggaatctat aatggtagtt aatctatgag
     3481 caataactaa aaccgaacgt ccttttttta agttttctat ggatttctcc acatagtact
     3541 cggatttggt atccaaggca cttgtaggtt cgtctaacat tatcaaaggt gcatctttaa
     3601 gaagagccct ggctaccgct attctctgct tctgaccgcc tgatactttt gctcctcgtt
     3661 ctccgacatc tttaaccagt ttgtctatat atgccttttt ggatgcatct tccaccattt
     3721 catcggtagc ttccaaatta cccatcttta tgttattgat aatgtcatca tcaaacaggt
     3781 atacatcctg agttacaacg gtcatatatt ttctcaacgc ttttaagttc caggacctga
     3841 tactatgacc gaaaacatat atttctcctg taaaattctc ataatatccg cagataagct
     3901 tgaatatggt acttttaccg cttccgctca ctccgcacaa tgctaatctg gagtttttat
     3961 gcaatttgaa actgacatcc ttaagtaaag gtatgtcttc atgataggag aaaccgacat
     4021 ttttgaactc cactatgata tccgagtctt gggtaccgaa atcctcacca tcacttcgtt
     4081 cttcttttgt ttcaagaaat ttaaaatatc tttccgcagt accggtataa cctactatat
     4141 ccgcccatcc cataccggta gttctgaagg tttgtatgag aggccaaagc agattgctga
     4201 atattacata ctgccctatc gtcaattcgt ttcttgcaat aaaaaacatt gatgcaaaca
     4261 tgcaaacacc ttgcggaata agcatcatta agacagtaag cgctctgttc ttggtcataa
     4321 caaaactgtc tttatacgat tcctccgcta tatcctgaca agactgctga acctttgccg
     4381 tatgagattt gtgcatgtta aatgatttat atatatcgtt accttcaaca aaatcccgga
     4441 tatatgagtt caatacggcc gtatattcct gtatcttatt ctttacttcc cttaaaggct
     4501 tggaggcttt atcaattaga tacattatta aagggattac ggttaaggaa aatacggtca
     4561 gtttccagtt aatcctgaca agaaaaaaca tggaaagtat aaaggatacg ggattgtagg
     4621 aaagaaatcc cgctaccgta ttacccatga atgtctttat cttttccaca tcgtctacca
     4681 atgtgcttat ggtatcggct gaatgtacat tctcataata tcccatctct aacttatcta
     4741 tcttgtcata tacattcatg gcaagagttt tgtgtgttct taactcgtac tgtgaagcta
     4801 tcaagggatt aacaaactga agaatgaatc ttattacaaa aattgcagat aaagcatata
     4861 taagagtata aagcctgtta taatcgcccg acaaagctac atccaatacc gatcccgtta
     4921 caaagaatat gacgactgcc aatattcccg atactacacc gccgaaccat cttccaaaag
     4981 cccatttctt atgttccgca gattctttgt ataactttac catcgttttg ataacatatt
     5041 ttacatcggt cttttctttc ttttcaggtg tttctttatc tttttttgcc atatttgtcc
     5101 tcacacaaaa tccattttta tctgtccatg gtcggtttta atctccgctg ctgctattat
     5161 atccgattta tatgcataac ggctttcacc ttttgcataa ataatccttc cctgcatgtt
     5221 tctttccacc gactgtgctt ttaaattact tcccagacgt atagaaccgt gttttccggc
     5281 tgagcattca aacaccgctc cgaaattgta gttaatatta actgttgttg aaccgtttgt
     5341 tgtttccccg
//
LOCUS       DAOSOM010000074         5177 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_16166_length_5177_cov_4.837954, whole genome shotgun sequence.
ACCESSION   DAOSOM010000074 DAOSOM010000000
VERSION     DAOSOM010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5177
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16166_length_5177_cov_4.837954"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3395)
                     /locus_tag="PLF14_09785"
     CDS             complement(<1..3395)
                     /locus_tag="PLF14_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71610.1"
                     /translation="MRKKRITVIISVILAALLLAIGVYAYYEYYGKYSALIQVNGVIR
                     LNAFAALDEEYREPQYGYGVTPENPFVIDNQDRMNNLIVLNNGGKLLSAKEAYGADKF
                     YFAFNFTEQPTPQVLSLSGITQEPVGNNDYPFIDNLTGLLYAYLVSDSEYVYLSAGGS
                     ADVVDEGGTTTINGQSVTIVSGTATTAEYIKVPDFYITLYPAIADNPYLIRTSDDDYY
                     LPVTSLLAAPQIVSDVTVKAPEDQIDVGFISCIGREYEMVDDGQGGQTVNLESIVSRS
                     SVSDIILYNITIDCTESQPNAIKTALDAIWESILSAVPGHIFADTEAQTDRRHIGLFA
                     GHIDGMAHNITVAGEGVIKIDGADINTYSRFTTVGYIDDRAIINNIPFSELTISGTGA
                     ALLSQPFFADSIYELAQKQPNVYEYPLKNINGQLGIWSGITTTSQGGQNINHFSQGIF
                     KFILSHENDTVRDIWQGRGNINLLNAEGFNVTQSVLYCANEYRYSESQQGGGSLVSTG
                     ASANETRYAGVSTLTASGNILDRGQYIIVAKIYDEAVSDYKYYALKIIAEIGQDGTIV
                     YNFDDSEKVDVTTYINGGAEDGIYSSCIWYNNTDTQDPTFNNTRFRSQYLSVSQDGSS
                     LDLTLTNDPEGAATFRASVADSTFSYSIIEAVGSGYVTTRYYLNFNTDTSSFYFSTEN
                     NTVIEIYQISNGFNLELVDDIADLTLNDDYIITAQYGGNHYLLGVGTSEGVGGAQVVD
                     GTFATDLSLQPMPTNWNIGNYHALKRYVWNASYASSGVVAFHEKLSSTYYLGYSGSSL
                     ALTQQQINWTYTQGAANGGTLRTAGSYLTYINGIGNCSFTLGVNEYTIYIYKLVPDDK
                     EPLYHTYRGARLLSAESSVVQKGQYMIAANTGSSYQALVMTNTNTLGVADVTVYANGT
                     ATKAQLDLLLDNGTNEGYKWEVGTTSPKPSLQNIKYASDGSYLSNSANTQPALSTIPV
                     QWMYDVVSGRLYYRSSDTLYYMAYNNSTTTFVITSDPEAAGLIYEIRLYKVIYEYEYA
                     GVAPVQDMSYNDATYSSYHTSNTGPVYQTYIGPNKYYFLLTAPLTSMATNPPYALGSY
                     SYDDQGTVESINIAPTSTYDAGNSILTTTCDLSYYRWVLDVRTT"
     gene            complement(3392..4267)
                     /locus_tag="PLF14_09790"
     CDS             complement(3392..4267)
                     /locus_tag="PLF14_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71611.1"
                     /translation="MKEFVKNSKGILLTALSIVLLIAILITASLAWFYFPTAESVTIN
                     SDIQHEIDVDLYQYSESLGYFVQAGLNDAEDTVVATCGTEATFVEWGGIFYPTNDITD
                     YYMLMLTYPDDDFLNGNLEAYVGMMLQSGMNGTEANYKIVYTTIEYAISGAADINPAS
                     TDAITTVQAAEYTRIFEPPVEAPTPPEGAPLDYIEQDHLSITLKNLTEEQYVYTYGGE
                     SKCRLVLFFRIKSDASRISQTMTWYHVPFYVLQIDTKNIYTFTCNFRSIPYKSADAGF
                     DRIYVLAHDRKERPL"
     gene            complement(4264..5013)
                     /locus_tag="PLF14_09795"
     CDS             complement(4264..5013)
                     /locus_tag="PLF14_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71612.1"
                     /translation="MPKDMIKKSIIAFSALLLLIIACLFRFGSISTAWFREDRKSGMT
                     ASNIYSITQCETSSVAPFKNNEVAFDANCTLDDIMGNYEKTIIPGDVLYYAFLILDVD
                     IVSYQTFMMEFAGLLVLEGEGGFNAESFAGNLCIEENTVIFAVLEGTPDGMGGETYEL
                     YPYDNGNAFYSITNTGVDHIYTEENGLQADLQFTLPEGVAYPAGTNGKADILVIVPVW
                     YMDTGANQNIEMNCSLTIEGCVLYPFTGDGQ"
BASE COUNT         1400 a         1179 c         1003 g         1595 t
ORIGIN      
        1 gtcgttctca catccaatac ccatctgtaa taggaaaggt cgcatgtagt ggtcaatatg
       61 ctgtttccgg catcataggt tgacgtgggt gcaatattta tcgattctac cgtaccctga
      121 tcatcataac tgtacgaccc gagtgcatat gggggattgg tagccatgct cgttaaagga
      181 gctgtaagta gaaagtaata cttattcggt cctatatatg tctgatatac cggacctgta
      241 tttgaagtgt gatatgacga gtatgtggca tcgttatatg acatatcctg aaccggagca
      301 acaccggcat attcatactc atatattacc ttgtaaagcc ttatttcata tatcagtccc
      361 gctgcttcgg gatcggatgt aatcacaaag gttgttgtgc tattgttata tgccatataa
      421 tataatgtgt ccgaagacct gtaatacaac ctacccgata cgacatcgta catccactga
      481 acgggtattg tagataaagc cggctgtgta ttggcggagt tggacagata tgaaccgtct
      541 gaggcatatt taatattctg caatgaaggt ttcggtgatg tagtgcctac ctcccatttg
      601 tacccttcat ttgtcccgtt gtccaaaaga agatcaagtt gagccttagt agctgttccg
      661 tttgcataaa ctgttacgtc tgcaacaccc aatgtgtttg tatttgtcat taccaaagcc
      721 tgatacgagc taccggtatt ggctgcaatc atatactggc ctttctgtac gacagaactc
      781 tctgcagaca gaagccttgc acctctgtat gtgtgataca gcggttcctt atcgtccggc
      841 accagtttgt atatatatat agtgtattca ttaacaccca atgtgaagct gcaatttcca
      901 ataccgttga tatatgtaag gtaactgccg gctgtcctga gcgtaccccc atttgccgcc
      961 ccctgtgtat atgtccaatt aatctgctgt tgcgtcagag ctaaagaaga accggaatag
     1021 cccaaatagt atgtggaact cagtttttca tgaaatgcca ctactcccga tgatgcgtag
     1081 gatgcattcc atacatacct tttcaaagca tgataattac ctatattcca gttggtaggc
     1141 ataggctgca aagaaagatc ggtggcgaaa gtaccgtcta ccacctgggc tcctcctaca
     1201 ccctccgatg tgcctacacc taaaaggtaa tgatttccgc catactgcgc cgtaattata
     1261 tagtcatcat taagcgttaa atccgctata tcatccacca attccagatt gaaaccgttg
     1321 gatatctggt atatttctat tactgtatta ttttccgtgg aaaaatagaa actggatgta
     1381 tcggtattga agttgagata gtatcttgtt gtaacatatc cgctccccac tgcctctatt
     1441 attgagtatg aaaatgtact gtccgctaca ctggctctga atgttgcggc accttccgga
     1501 tcgtttgtaa gtgtcaaatc cagtgatgaa ccatcctgtg aaaccgataa gtattgcgat
     1561 ctgaaacgtg tattattgaa agtaggatcc tgcgtatcgg tattgttgta ccatatacag
     1621 cttgaataaa taccgtcctc ggcaccgccg tttatatatg tggtgacatc cactttttcc
     1681 gagtcgtcaa agttgtatac aattgttccg tcctgaccta tttcggcaat aatcttcaac
     1741 gcataatact tgtaatccga caccgcttca tcatatatct tggcaacaat aatgtattgt
     1801 cctctgtcaa gtatgttgcc cgatgccgtt aaagtactta ctccggcata tcttgtctcg
     1861 ttggcagatg caccggtaga taccagactt ccgcctccct gctggctttc gctatatctg
     1921 tattcatttg cacaatacag aaccgattgt gttacattaa atccctctgc attcagaaga
     1981 tttatatttc ctcttccctg ccatatgtca cgtacagtgt cattctcatg agaaagtatg
     2041 aatttgaata tgccttgtga gaaatgattt atattctgtc cgccctgaga tgtagtggtt
     2101 ataccgctcc atatgcccaa ctgtccgttt atatttttca aaggatactc ataaacattc
     2161 ggttgttttt gtgcaagttc atatatcgag tcggcaaaga aaggttgtga caataatgca
     2221 gcacccgtac cgcttattgt cagttcggag aaaggtatat tgtttattat tgccctgtca
     2281 tcgatatagc ctacggtggt aaaacgcgaa taggtgttaa tatccgcacc gtctattttt
     2341 ataacgccct cacctgctac cgttatattg tgtgccatac cgtcaatatg gcctgcaaac
     2401 aatcctatat gccttctatc ggtctgcgcc tcggtatcgg caaatatatg gccgggaacg
     2461 gcactgagta ttgattccca tatagcatcc aaagccgttt ttatggcatt gggttgtgat
     2521 tccgtgcaat ctatcgttat attataaagt atgatatcac tgaccgaaga tcggcttact
     2581 atactctcca gatttaccgt ctggccgcct tgtccgtcat ccaccatttc gtattctctt
     2641 cctatacaac tgatgaatcc cacatctatc tgatcttcag gtgccttaac cgtaacatcg
     2701 gatactatct ggggagcggc aagtaatgaa gttaccggta aatagtaatc atcatccgat
     2761 gtccttatta aataaggatt atctgcaata gccggataaa gggtaatata gaaatccggt
     2821 acttttatat attccgcagt agtggcggtt ccgcttacaa tagtaaccga ttgtccgtta
     2881 atggttgtgg tgcctccctc atctaccaca tccgccgatc cgccggcact cagatataca
     2941 tactcggaat cacttaccag atatgcataa agaagccccg tcaggttgtc tataaaggga
     3001 taatcattgt tgcctaccgg ttcctgcgta ataccggaaa gactcaaaac ttgaggcgta
     3061 ggctgctcgg taaaattaaa tgcaaagtag aacttatcgg ctccgtatgc ctcttttgca
     3121 ctgagaagct ttccgccgtt attgagaaca ataaggttgt tcattctgtc ctggttgtcg
     3181 attacgaacg gattttccgg tgtaactccg tacccgtact gcggttctct gtactcttca
     3241 tccagcgcgg cgaatgcatt caacctaata actccgttaa cctgaattaa agcactgtac
     3301 ttaccgtaat attcatagta agcatatacg cctatcgcca aaagcagagc tgccagtata
     3361 acacttatta ttactgtaat gcgctttttt ctcataacgg tcgttccttt ctgtcatgtg
     3421 ccaaaacata tattctgtca aaacccgcat cggcggattt atacggtata ctcctgaaat
     3481 tacaggtaaa tgtatatata ttctttgtat caatctgaag aacataaaag ggaacatgat
     3541 accaggtcat agtctgactt atacgtgatg catcgctttt tatgcggaaa aacagcacaa
     3601 gacggcattt ggattccccg ccatacgtat atacatattg ctcttccgtc aaattcttca
     3661 atgttatact taaatggtcc tgttcaatat agtctaaagg agcaccttcc ggaggcgtgg
     3721 gcgcttctac gggaggctcg aatattcttg tatattccgc cgcctgtacc gttgttatag
     3781 catctgtaga agccgggtta atgtctgcgg caccggatat tgcatactct atagtggtat
     3841 atacaatctt gtaattggcc tcagtcccgt tcatgcccga ttggagcatc atgcctacat
     3901 atgcttcaag atttccgttc agaaaatcgt cgtcgggata tgtaagcata agcatatagt
     3961 agtccgtaat gtcgttagtg ggataaaata ttccgcccca ttcaacaaaa gttgcttccg
     4021 ttccgcaggt tgctacaacg gtatcttccg catcgtttaa tcctgcctgc acaaagtaac
     4081 ctaaactttc gctgtattga tataagtcca catctatttc gtgttgaata tccgaattaa
     4141 ttgtcacact ttcggcagtc gggaaataga accatgccaa actggcggtg ataagtatcg
     4201 ctataagtaa aactatactt aaagcggtta acagtatccc tttgctgttt ttcacaaatt
     4261 ctttcattgc ccgtcccccg taaacggata caatacgcat ccttctattg ttaaagaaca
     4321 gttcatctct atattctgat tagctccggt atccatatac cagaccggta caattaccaa
     4381 tatgtccgct ttaccgttag ttcctgcggg atatgccacc ccttcaggta atgtaaactg
     4441 aaggtctgcc tgcaaaccgt tttcttccgt ataaatatgg tcaacaccgg tattggttat
     4501 gctgtaaaag gcatttccgt tatcatacgg gtataactca taagtttccc ctcccatacc
     4561 gtccggtgtt ccctccagca cggcaaatat aacggtgttt tcctcaatac acaaattacc
     4621 ggcaaaagat tcggcattga aacctccttc tccttccaac accaacagcc ccgcaaactc
     4681 catcataaag gtttgatatg acacgatatc gacatctaaa ataagaaatg cataataaag
     4741 tacatcaccg ggaattattg ttttttcgta attgcccata atatcatcca gagtgcagtt
     4801 tgcatcaaat gcaacttcat tattcttgaa aggtgcaacg ctgcttgtct cacattgggt
     4861 aattgaatat atattggatg ccgtcatacc ggattttctg tcttctctga accatgcggt
     4921 ggaaatactg ccgaaccgga ataagcatgc aattataagt agcaataaag cggaaaatgc
     4981 gattatgctt tttttaatca tatccttcgg catttacaca tcctccttct ttacggtttc
     5041 ggcggttgta ttggcatatt gctcttttaa cttcttttcc tgtatgagta ataacatcct
     5101 tataacctga ataataatta caatgaccag cggaattata accaaaagga ggaaacctgt
     5161 ccgggtcctc attatga
//
LOCUS       DAOSOM010000075         4888 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_17418_length_4888_cov_8.477964, whole genome shotgun sequence.
ACCESSION   DAOSOM010000075 DAOSOM010000000
VERSION     DAOSOM010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17418_length_4888_cov_8.477964"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..2454
                     /locus_tag="PLF14_09800"
     CDS             163..2454
                     /locus_tag="PLF14_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009441017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HOT71613.1"
                     /translation="MASAMQELMDKKIWFPWRYVTDKDGKVKKLPFAASGGATGTDDT
                     YSSTWVIYDEAVSAKNKFHAAGVGFKLPEGYFFLDIDHRKLTDPLVQTLLVRFDSYAE
                     HSVSGGGIHIYGKCDLSKLPTYIDVKGKVRLDKQFYQKNSKIDLELYVGGITNRFAAY
                     TGNIIEDKPLNDCTLAVLTTLDKNMRKTKKINYSKKRDGDRAAFDIVCNLRKQKNGEK
                     FKKLYDDGDISEYGSQSEADAALCALIAFRTGPKFGEIDDIFRSSALYREKWERDDYR
                     EVTIAAGIEACHGIFHKSKMEHPYFIKFNEQTGEAYVSVPLLAKYVREHLCYVLVRDN
                     GKQGLMKYIYEKGCYRLYADNMLMGIIKQYIADYDEELVKMGKVSETLQHITTDLNYI
                     SQEELNADEDLINFSNGLLRITANNAILAPHSPDILSTIQIPCSWTGRETPTPVFDRY
                     MHTLTNGDKAIEQLLLEFIGVCISNIKGWRMKKALFLVGIGDTGKSQLKSLVERLLGK
                     GNFIGIDLKEIEARFGTGAVYGTRLAGSSDMSFLSVDELKTFKKITGGDSLYAEFKGQ
                     QAFEFTYNGLLWFCMNKLPKFGGDDGKWVYDRIMIVRCPNVIPKDKQDKTLLDKMYAE
                     RDGIVFKAVKALQTVIANGYRFSEPNSVSLAREKYMAENNTVVSFFEECICEWPDGKI
                     NKHCTTGRIYKVYQGWCRENNNGFAKTAKEFRETLATYLGTDYSTITTRHNGNTYYKN
                     YSLTNEAKEQFAKEYGYDGTEFL"
     gene            2727..2990
                     /locus_tag="PLF14_09805"
     CDS             2727..2990
                     /locus_tag="PLF14_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71614.1"
                     /translation="MNQIRVIGVMDAESEITKHSSPSDFGDDLYDGISLYRRKDMKPV
                     VLFVSKNAEPARWKAVDGASEFYFCSYAEAIRFCKIRGYIFMK"
     gene            3104..3415
                     /locus_tag="PLF14_09810"
     CDS             3104..3415
                     /locus_tag="PLF14_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71615.1"
                     /translation="MANYPLTKMNKEGTLLRPQHSYYSDEYAQSTCDLFLSDSIVEDE
                     HGKLHKYYRLHAKQDHNMEMAFAYDIHCPDCNVGMLKQISRPLSYNELGLYRCPVCDK
                     K"
     gene            3426..4379
                     /locus_tag="PLF14_09815"
     CDS             3426..4379
                     /locus_tag="PLF14_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009728910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HOT71616.1"
                     /translation="MMSTYTERLPYTGSPEYDKHFWNAMRGHKTSLDELSKGYNTGTG
                     LYAMPNSSDNKYMNALEKESLFRGIGTVIKAYGSGYRIFAKDSKELAEWVAENDAIPI
                     YESIEDFTINTVDSWKLAALVKMDEAFIHDASFDIENYLIKRFAKNFGRAEDNAFING
                     NGTEMPTGILNATGGAEVATTIDEITYDDVISLYFSVKPEYRKNAVWLMNDVTALALR
                     TLKDNAGNYLWRDSDDTILGKKVIMTEFMPDVESGSKPIAFGDFSYSWVIGRRPIGVR
                     TLTEKFAALDQLGYLAYEFLDGKLIRPEAVKVIQINTESIG"
     gene            4658..4816
                     /locus_tag="PLF14_09820"
     CDS             4658..4816
                     /locus_tag="PLF14_09820"
                     /inference="COORDINATES: protein motif:HMM:NF025565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-encoded TnpW family protein"
                     /protein_id="HOT71617.1"
                     /translation="MDDKTISVTEKVIGDTLYIVESAVSNAAKETAYDKLKRMMLNDT
                     KSLEIKAS"
BASE COUNT         1534 a         1026 c         1109 g         1219 t
ORIGIN      
        1 agcctaccaa aagcctacga acttgtaaaa tcgccgggat tccccaccat acgaattggt
       61 acgaggattt taattccggt tgatgcctat aaggagtggt tactcagaaa ctcggcacac
      121 agataatgga aaagcaaatt ttacggagag gaggtgaacg agatggcgag tgcaatgcag
      181 gaactgatgg ataagaaaat atggtttccg tggagatatg tgactgataa ggacggcaag
      241 gtgaaaaaac ttccctttgc tgcaagcgga ggtgcaacag gtacagatga cacttacagt
      301 agtacttggg ttatatatga tgaagctgtt tcagcgaaaa ataagttcca cgctgccggt
      361 gtcggtttca aactaccgga aggctacttt tttctggata ttgaccacag gaaactcaca
      421 gacccgctgg tgcagacatt gcttgtccgg tttgactcct acgcagagca ttctgtgagc
      481 ggaggcggta tccatattta cggcaagtgc gacttgtcaa agttgccaac atatatagat
      541 gttaaaggca aggtcagact ggataagcag ttctatcaga agaattccaa aatcgacctt
      601 gaactttatg tcggaggtat cacaaaccgc ttcgcggcct ataccggcaa tatcatcgaa
      661 gataagcctc ttaatgattg taccttagca gtccttacaa cgcttgataa aaatatgcga
      721 aagaccaaga agataaatta cagcaaaaaa cgtgacggtg accgcgctgc tttcgatatt
      781 gtctgtaatc tccgtaaaca gaaaaacgga gaaaaattca agaaactata tgatgacggc
      841 gatatctccg agtatggctc acagtcagaa gcggacgcag cattatgtgc cttaatagca
      901 tttcgtactg gacccaaatt tggggagatt gatgatatct tccgcagttc tgctctctat
      961 cgcgaaaaat gggagcggga tgattacaga gaagttacca ttgctgccgg gattgaggcc
     1021 tgtcatggta tctttcataa atccaagatg gaacatccgt atttcatcaa gtttaatgag
     1081 cagactggag aggcttatgt cagcgttcct cttcttgcaa aatatgtgcg agaacacctc
     1141 tgctatgtgc ttgtaaggga taacggtaaa cagggactta tgaaatacat ttatgagaaa
     1201 ggttgctacc ggctttatgc cgacaatatg ctgatgggca ttatcaagca gtacattgca
     1261 gattatgatg aggaacttgt caaaatggga aaagtcagtg aaaccttgca gcatatcacc
     1321 accgacctta actatatcag ccaagaggag ttaaacgccg atgaggatct aatcaacttc
     1381 agcaatggtc tgctccgtat tactgcaaac aatgccattc ttgctccaca cagtccggat
     1441 attctctcca ccatacaaat cccatgtagc tggacaggca gagaaacacc cacaccagtg
     1501 tttgacagat atatgcacac cctcaccaac ggtgataaag ccatagagca actgctcctt
     1561 gaatttatcg gagtatgtat ctcaaacatc aagggatgga gaatgaaaaa agcacttttc
     1621 cttgttggca ttggtgatac cggcaaatct cagctaaaga gccttgtgga gcgtctgctt
     1681 ggcaaaggga actttatcgg tattgacctt aaagaaatag aagccagatt cggcaccggt
     1741 gcagtttacg gcacacgcct tgccggtagc tccgatatga gttttctctc cgtggatgaa
     1801 ctgaaaacat tcaagaaaat aaccggtggc gatagtctgt atgccgagtt caaaggacag
     1861 caagccttcg agttcaccta taacggcctg ctgtggtttt gtatgaacaa acttccaaag
     1921 ttcggcggtg atgatggtaa atgggtctat gaccgcatta tgatagtgag atgcccaaat
     1981 gtcatcccca aagacaagca ggataaaaca ctcctcgata aaatgtatgc cgagcgtgac
     2041 ggcatcgtct tcaaagcggt aaaggccctg cagacagtca tcgccaatgg ctacagattc
     2101 tcggaaccga acagcgtaag cctggcaaga gaaaaatata tggcggagaa caatacagta
     2161 gtctcctttt ttgaggagtg tatttgtgaa tggcctgacg gaaaaatcaa caaacactgt
     2221 accacaggcc gcatttacaa agtttatcaa ggttggtgcc gggaaaataa taatggcttt
     2281 gctaagactg ctaaagagtt ccgggagacc ctcgctacat acctcggaac ggattattcg
     2341 acaatcacta cccggcataa tggcaatacc tactataaga actattcgct cacgaatgag
     2401 gcaaaagagc agtttgcaaa ggaatacggc tatgacggaa ctgagttctt ataaaaatgg
     2461 tagcagtagt agcagtgccg gtagcagttg tagcagttct tataacttct cagaaaaatt
     2521 tcaataaagt tagtaaaaaa atataagaat acaaagattg tatgaagtgt gagtataaac
     2581 acgcttagtg ctgctacttc tgctaccaac cgactgaaaa cccttgatat acatcgcttt
     2641 tctgaagtag cagtaccaaa aaactctgct accagttctg ctactactgc tacaaaaaat
     2701 ctcaattcga aaggaggaaa aaggatatga accaaatcag agttataggc gtgatggatg
     2761 ccgagagcga aataaccaaa catagcagtc catcggattt tggtgatgac ctctatgatg
     2821 gtatctcgct ctatcgccgc aaggatatga aaccggtagt gctattcgtg agcaaaaacg
     2881 ctgaacctgc ccgatggaag gccgtggatg gagcttccga gttctacttc tgttcatacg
     2941 cagaggctat aagattctgc aagatacgcg gatacatttt catgaaatga ggaaagcagc
     3001 gttaatgcag gaacctttta ccctgacagc tgaaatggca ttagctcaaa aatgagccaa
     3061 cgtttttcat tacatccaac aataatttta ggaggaattt aaaatggcaa actatccact
     3121 tacaaaaatg aacaaggaag gaacactcct tcgcccgcaa cactcatact actctgatga
     3181 atatgcacag agcacgtgtg atctattcct gtcagattcc atcgttgaag acgagcatgg
     3241 aaaactgcac aagtattatc gtctccacgc aaaacaggat cacaatatgg aaatggcttt
     3301 tgcctatgat atccattgcc cagattgcaa tgtcggtatg ctaaaacaga tcagcagacc
     3361 actcagctat aacgaactag gactatacag atgccctgtt tgtgacaaga aataaggagg
     3421 gaaaaatgat gagcacttac actgaaagat taccttatac cgggtcaccg gaatatgata
     3481 agcacttctg gaacgctatg cgcggccata agaccagttt agatgaactt tcaaaaggct
     3541 acaacaccgg aactggtctg tatgcaatgc caaattcatc ggataacaag tacatgaacg
     3601 ctcttgaaaa ggaaagcctg ttccgcggta ttggtacagt cattaaagca tacggttctg
     3661 gctaccgtat tttcgccaaa gacagtaaag aacttgctga gtgggtagca gaaaatgatg
     3721 ccatccccat ttatgaaagt atcgaagatt tcaccattaa caccgtagac agttggaaat
     3781 tggctgcttt ggtaaagatg gacgaagcct tcatacacga tgccagcttt gatattgaga
     3841 attatcttat caaacgcttt gccaagaact tcggtagagc cgaggataat gctttcatca
     3901 atggcaacgg aactgaaatg cccacaggta ttttgaatgc caccggtgga gcagaagttg
     3961 ctacgacaat tgatgaaata acatatgatg atgttatcag cttgtacttc tcagtaaaac
     4021 cggagtaccg caaaaatgca gtatggctga tgaacgatgt aactgcactt gcacttcgta
     4081 ctttgaagga caatgcaggc aactacctct ggcgagacag cgacgatacc attcttggca
     4141 agaaggtaat aatgactgag tttatgccgg atgtggagtc agggtccaag cccatcgctt
     4201 tcggtgactt cagttattcc tgggttatcg gcaggagacc catcggcgtt aggacactta
     4261 ccgagaagtt cgctgctctt gaccagcttg gatatttggc ctatgagttc cttgacggta
     4321 aacttatacg accagaggcc gtaaaggtaa ttcagataaa caccgaatcc atcggttaaa
     4381 taaattacga gaaaaggcac agtggaatga tctgctgtgc ctttttctca tagaaatatg
     4441 tattttccat gattgaatct acaacacatt tacccttatt gtgtgcagtt tatctcggtt
     4501 gtggctttaa taccccgttt gaatctgcgt ttttttacgc gttaccccac gccgctgtcc
     4561 gagatgaaaa gttgtagaga tcagaccacc cccaaccggt caatagacac ttaagggagt
     4621 aacgattcgt tacgccatat cgaaaggagg aatccttatg gatgataaaa ccatcagtgt
     4681 taccgaaaaa gttatcggtg acacgctgta cattgtagaa tcggcagtca gcaatgctgc
     4741 caaagagact gcttatgaca agctaaaacg catgatgctg aacgacacca aaagtcttga
     4801 aataaaggct tcttagccca tatcaacttg acttcttcac agtagtacgg gaatatagag
     4861 taccgcttga agactgtcgg aaaggagg
//
LOCUS       DAOSOM010000076         4633 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_18607_length_4633_cov_3.440149, whole genome shotgun sequence.
ACCESSION   DAOSOM010000076 DAOSOM010000000
VERSION     DAOSOM010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4633
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18607_length_4633_cov_3.440149"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1745
                     /locus_tag="PLF14_09825"
     CDS             <1..1745
                     /locus_tag="PLF14_09825"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT71618.1"
                     /translation="KVRHTVKRLYTYSKKAFWKWGIWRWIGGLFSASLNILIARMIGQ
                     MLDIAVGGKWSEAITFIVLVSVFVIGRSVIGYTNSLTSYSYEAHAGYIMRCAAMDKIN
                     ALPIGYFESKHTGTVISRIMNDIGKMQEFLPNSIASVWSHTPVNIFFGLTLLFMIDWR
                     LTLIVVSVVMITTFILSKISVPLVKYTDAEQQSKVKYNAYLRDFIEGNDIYKIYGMKK
                     THGKKFNSAVEEQFKCSKAINRQRSKIRSIAGLNYYLPWVLSYIIGSFFVAKGDITIG
                     ELYAFAAILPNVTKVAWQIGDTVASIAEVGGIAGHFFKLIDQPEERQDGDDYSNVSDV
                     SITFDSVSFSYSAEFDVLSGCSFIVPKASTVALVGGSGSGKTTIFKLICGYYEEYSGD
                     VKIGGYSLRKWNLHALRDNITYMSQESYLFDDTIMENIRNGRPEATDDEVIEVAKAAY
                     ADEFIMETVNGYNTVLGERGIMLSGGQRQRIAIARAMLRNTPIILLDEPTSALDTRSE
                     HYVQLALNNLAKSRTVFVIAHRLSTIENADNILVLDNGRIVEQGTHSKLIKQDSRYKE
                     LYFAQIAEVNADET"
     gene            1735..3483
                     /locus_tag="PLF14_09830"
     CDS             1735..3483
                     /locus_tag="PLF14_09830"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT71619.1"
                     /translation="MKRNFFKDFSRFAKLCGKESRMLFTIAFLEGIISGALGTIFQAQ
                     ALKYIIAYFIDKQSNALANAFIMIVLTVLVGFVFRPVLDYFGNVIVSKIIASLRIRLY
                     EHLTKLPASYFAGTHSGDIAARINGDVDAFAGACYTIRSFIESIIPAIVLMPYLLVLD
                     IRMALIIGLVGLAFLILTAKVIEPLRIRQKKIRSEIAKMSVSATDGIMGFQVIKSNNL
                     EEKFSNSFKEHAGTLLNTERDFARVNAIVGFFNIFTWCAGEAFIAIFGLVFVLKSTLA
                     VTNLAALVSAGRSVIGIFTDFSSLPVQLQQAFAGVDRVQELCDIEPEPERYPISGNFE
                     DCGISVKNLSFSYNKETKVLNNISINAKRGKTIALVGDSGSGKTTLIKILAGIYCADE
                     GEITVLGRPLGDYTLDEYRKKIAYVPQNAYIFDGSIEENISYGREGASLNEIKLAAQK
                     SNAELFILQKPAQYDTPVGERGIKLSGGERQRIAIARAILKNSEILLLDEATSSLDSE
                     SELAIQETLDSLIQQHTSVVVAHRLSTIINADCIYMMQKGKIIGFGKHEQLLDSCIKY
                     RELYYRLFASKEGQSK"
     gene            3585..>4633
                     /locus_tag="PLF14_09835"
     CDS             3585..>4633
                     /locus_tag="PLF14_09835"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOT71620.1"
                     /translation="MFNKINVKTFVKRFDYTLLGLVLALTSIGAVALPSVVDTMNSGP
                     SIIKTQMFSIALGLILCIGFSILDYSFLRYWGIAFYIIGILMLIYVIPFGYGRDDPNI
                     GSNSWIRLFGAFSFQPSELMKVAYMMFLPSQFELLKEEFSLKRLIFIAVFGLMPIGLI
                     MLQPDLGTATVFAFSFAVLIFVYGIKYRYLLISIGVLAASLPIAWLFLDDWQKDRIRV
                     FIDPNFDPSGAGYQTSKSIIALGSGGWTGAGLYNGIRTQSNGIPVKESDFIFSAIGEE
                     MGFLGCAAIIILAFAIIIRCIHIASKCSSYYGQYIVAGMAAMIAFHFIENIGMNIELM
                     PVTGIPLPFVSAGGT"
BASE COUNT         1338 a          801 c         1062 g         1432 t
ORIGIN      
        1 agaaggtccg gcacaccgtg aaacgcttat atacctattc aaagaaagca ttttggaaat
       61 ggggaatatg gcgttggata ggcggcttat tttctgcttc actgaatata ctgattgcac
      121 gtatgattgg gcagatgctg gatatagctg tgggaggtaa atggagtgaa gccattactt
      181 ttattgttct ggtctccgtg tttgtaattg gcagaagtgt tataggatat acaaattctc
      241 ttaccagtta tagttatgaa gcacatgcgg gatatattat gcgttgtgcg gcaatggata
      301 aaatcaatgc acttccgata ggatattttg agtccaagca tacagggacg gttatttcgc
      361 gtataatgaa tgatatcggt aagatgcagg aatttcttcc gaattccata gctagtgtat
      421 ggagtcacac tcctgttaac atatttttcg gtttaacact actgtttatg attgattggc
      481 gactgacatt gattgtagtc agtgttgtaa tgataacaac tttcatacta agcaagattt
      541 ccgtaccctt agtcaaatac acagatgcag aacagcagag taaagttaag tacaatgcat
      601 atctccgtga ttttattgaa ggaaatgata tttacaaaat ctacggaatg aaaaagacgc
      661 acggcaaaaa gtttaactcc gctgtggaag aacagtttaa atgcagtaaa gcaatcaacc
      721 gtcaaagaag taagataaga tctattgccg gattgaatta ttacttgcca tgggttctgt
      781 catacattat cggcagtttc tttgttgcta aaggagatat cactattggt gaactatatg
      841 cttttgctgc aatacttccc aatgtaacca aggttgcttg gcaaataggt gataccgtag
      901 cttcaatagc ggaagtaggc gggatagccg gacatttttt taaactaata gatcagcctg
      961 aagagcgaca agacggtgac gattacagca atgtaagtga cgtttctatt acatttgact
     1021 ctgtgagttt ttcttattct gcagaatttg atgtattgtc tggatgctct tttattgtac
     1081 ccaaagcctc tactgttgcg ttggtaggcg gaagcggaag cggtaaaacg acaatattca
     1141 agctgatatg tgggtactat gaggaatata gcggagatgt gaagattggc gggtattcct
     1201 tgagaaaatg gaaccttcac gctttacgcg ataacattac atacatgagc caagagagct
     1261 atctgtttga tgacacaata atggagaata taagaaatgg tcgtcctgaa gctacagatg
     1321 atgaagttat tgaagttgca aaggctgctt atgcggatga gtttattatg gagaccgtga
     1381 acggatataa tacagtatta ggcgaacgcg gaataatgct ttcgggcgga cagcgacagc
     1441 gtatagccat tgcgagagca atgttgcgaa atacaccgat tatactgctc gatgaaccta
     1501 cttctgcact ggatacccgt tctgaacatt atgttcagct tgcattaaac aatttagcaa
     1561 aaagcaggac tgttttcgta atagcgcatc gcctttcaac tatagaaaat gcggataata
     1621 ttcttgtttt ggataatgga agaattgttg aacagggaac tcactcaaag cttattaagc
     1681 aggatagtcg ctacaaagag ctgtactttg ctcagattgc agaggtaaat gctgatgaaa
     1741 cgtaatttct tcaaagactt cagccgattt gcaaagctgt gcggaaaaga aagcagaatg
     1801 ctttttacta tagctttctt ggaggggata ataagcggtg ctttgggcac aatttttcaa
     1861 gctcaagcac ttaaatatat aattgcatat tttattgata agcaatcgaa tgctttggct
     1921 aatgctttca tcatgatagt ccttacagtg cttgtgggtt ttgtatttcg tcccgtttta
     1981 gactactttg gaaatgttat tgtaagtaag attattgctt cattgcgtat acgtctgtac
     2041 gagcatctta ctaaactgcc tgcatcatat tttgcaggta ctcatagcgg tgatattgca
     2101 gcccgtataa acggagatgt tgatgctttt gcaggcgcat gctacacaat ccggagcttt
     2161 atagaatcaa tcattcctgc aattgtttta atgccatatt tgctggtgct ggatattcgt
     2221 atggcactga ttataggttt agtcggattg gcctttttaa ttcttacagc caaggttatt
     2281 gaaccgctca gaatacggca aaagaaaata cgttccgaga ttgctaaaat gtccgtatcc
     2341 gctacagacg gtattatggg atttcaggtc attaaaagca ataatcttga ggaaaagttt
     2401 tctaatagtt ttaaggaaca tgctggaaca ctgttgaata cggaaagaga ctttgcaaga
     2461 gttaatgcta ttgtaggttt tttcaatata ttcacatggt gtgcaggaga agcatttata
     2521 gcaatattcg gattagtttt tgtgctgaag agtactcttg ctgtaaccaa tcttgccgct
     2581 ttggtatcag cgggccgaag tgttataggt atttttactg atttttcctc tctgccggtt
     2641 caactgcaac aagcttttgc cggcgtggac agggtacaag aactctgtga tatagaaccg
     2701 gaaccggagc gataccctat cagtggcaac tttgaagatt gcggaatcag tgttaaaaac
     2761 ctttcttttt cttataataa ggaaactaaa gttttgaaca acataagtat taatgcaaaa
     2821 agaggtaaga ccattgcttt agtaggggac agcggtagcg gtaagacaac cctcattaaa
     2881 atacttgccg gtatatactg tgcggatgaa ggcgaaatta cagtgctggg acgtcctctt
     2941 ggcgattaca ctcttgatga gtatcgtaaa aaaatagcat atgttccgca gaatgcatat
     3001 atttttgatg ggagcataga ggaaaatata tcttacggac gggaaggggc atcgttaaat
     3061 gagataaagc ttgcagcaca aaaatctaat gctgaattgt tcattctgca aaaacctgct
     3121 caatatgata ctccagtagg agagcgcgga atcaagcttt caggtggaga acgccagcgg
     3181 atagctattg ctcgcgctat acttaaaaac tctgaaatat tgttattgga cgaggcaact
     3241 tccagcttgg atagcgaatc ggaactggca attcaggaaa cattagatag tctcattcaa
     3301 cagcatacca gtgtagttgt tgcccaccgt ttaagtacaa ttataaatgc cgactgtatc
     3361 tatatgatgc aaaagggaaa gattataggt ttcggcaagc atgagcagct gttggattcc
     3421 tgcattaaat acagggaact gtattacaga ttgtttgcat caaaagaagg acagagtaaa
     3481 tagtttgctt tttgaggttg gaagtatagg cttttaatgt aaatgttagt cttgagcgac
     3541 agaatataat ggggtatact tactttgtgt gagaaaggct tgaaatgttc aacaagataa
     3601 acgtcaagac ttttgttaag agatttgatt ataccctatt gggactggtg ctggcattga
     3661 catcgatagg tgctgttgcc ctgcccagtg ttgtagatac catgaattcc ggaccttcca
     3721 ttattaaaac tcagatgttc agtattgctt taggattgat actgtgtata ggcttttcca
     3781 tactggatta ttcctttctt cggtactggg gaatagcatt ctatattatc ggtattttga
     3841 tgcttatata tgttattcct ttcggttacg gcagagatga tcccaatata gggtcaaaca
     3901 gctggataag acttttcgga gctttcagtt ttcagccttc ggagctgatg aaagttgcat
     3961 atatgatgtt tttaccgtca caattcgagt tattgaaaga ggagttctcc ctcaaacgac
     4021 ttatttttat agctgtcttc gggttgatgc ctataggact gataatgttg cagccggatt
     4081 taggtaccgc taccgttttc gcattttcct ttgcagttct tatctttgtt tacgggataa
     4141 aatacagata ccttcttatt tccatcggtg ttcttgcggc gtctcttccc atagcatggt
     4201 tgtttctgga tgactggcag aaagaccgga taagagtctt tatcgacccc aatttcgatc
     4261 cttcgggagc cggataccag acttccaagt ccattatcgc attgggcagc ggaggctgga
     4321 cgggtgccgg tctgtataac ggtatccgaa cgcaaagtaa cggtataccg gtaaaagaat
     4381 cggactttat cttttccgca ataggggagg agatgggatt cttaggttgt gccgcaatta
     4441 ttattctggc atttgcaatt atcattcggt gtattcatat cgcctcaaaa tgttccagtt
     4501 attacgggca atatatagtt gccggcatgg ccgccatgat tgcatttcat tttatcgaga
     4561 atataggtat gaatattgaa ctgatgcctg ttacgggaat acctctgccg tttgtttcgg
     4621 cgggaggtac taa
//
LOCUS       DAOSOM010000077         4540 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_19157_length_4540_cov_4.958305, whole genome shotgun sequence.
ACCESSION   DAOSOM010000077 DAOSOM010000000
VERSION     DAOSOM010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4540
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19157_length_4540_cov_4.958305"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /gene="queA"
                     /locus_tag="PLF14_09840"
     CDS             complement(<1..992)
                     /gene="queA"
                     /locus_tag="PLF14_09840"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOT71621.1"
                     /translation="MKKSDFYYDLPQQLIAQHPLEKRDNSRLLLLDKNSGEIRHTVFS
                     DIVNLLNKGDCLVLNETRVIPARLLGHVENSVGKIELLLLKRLTKTDWEVMTRPGKRA
                     AKGKRFVFGNGLIAAEVLDVINLGNRIVRFEYDGIFEEVLDKVGLVPLPPYITEKLED
                     KNRYQTVYAKYDGSSAAPTAGLHFTEELLDSLVKKGVDIVRVTLHVGMGTFRPVKAEN
                     IEEHIMHSEYYRIDRSAAESINAAKREGRKVIAVGTTSLRVLEGASDDFGVLHESEGE
                     TDIFIYPGYRFKVVDYLITNFHLPESTLIMLVSAMAGRENILSAYREAIKEKYR"
     gene            complement(994..2589)
                     /locus_tag="PLF14_09845"
     CDS             complement(994..2589)
                     /locus_tag="PLF14_09845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOT71622.1"
                     /translation="MNRKKRQSISILLAIICLMCFSCVNTANAVIYAPLYLQVGLYYG
                     NTAKSAYTVSSPFGLNMTININNREIDFVSTNNTLVVAKDTGDYSYHVIRDDNYNSYA
                     EALNVSNNMSSAGATSFPVYAASGFWQVWIGSYASSESAMNGIAGVASKYPGSYSTVA
                     YPSSARILVRDKASSIILFACDNPSAPIIAKTIATDSNSYMYIEGKPYRGNLEFTIFK
                     SNELSVLNNLLLEYYVYGVVALEIGSNSPMEALKAQAVVARTYALCQNKHQEYGFDVC
                     DKVCCQVYGGYNAENYRTNQATNETYGQVVTYAGQVVTPYYFSSSGGYTENSENVWVT
                     SVPYLKAVVDKYDINRTWTYSYTTSQMTDHMYKLGYRIGNVLKITIDSSSHTGRVLSM
                     TVHGSNNSVTFLKSNIRNAFPDFLPSQMFVLGGQSTVKVADAKGVATTQALDYASIRG
                     IYNTSLISSKTIGVRTNSGIVTVSLVTEDSNDKITISGRGSGHGVGMAQWGAINMADA
                     GYTYDEIIRYFYTGVKLESVTLR"
     gene            complement(2694..4391)
                     /locus_tag="PLF14_09850"
     CDS             complement(2694..4391)
                     /locus_tag="PLF14_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71623.1"
                     /translation="MKNNRKTGMTVIAILTVIVIALLSVSCKPQETLEGFNITTFNSD
                     IVIKRVDGQEPLNMPYRYAMPIIRAGLSEEVIGINISSVRCKIGRKGLSMSGYEKILV
                     EPDSSEVTRVINSIDYYKGITTLSGIIADKNDSKITIYEGYNEELLLETSQNYAIIPS
                     TLSKHINQELSDSDKVFYVKNNETRGAEPFKIIGEYTTNKEHDALYLSFYGMGKVAKG
                     AQIDIPDYIDCMEIDVNEGKDLSGLISVLNNYFADYNVLSQYEKRINRFNERYPYMFV
                     NTAGIKPVLIEEDTNFKKNIITVSRIDEKSDLMMSHLYGDALVKEYHEYSQYISDINI
                     GTGIKGVNPADYPTGTKYQPYGLKLMPLGMQQDRVWMSFNVPPYHKAVTSISEIKRDK
                     QGCEIYFYGNYRNRDLVKQREQDYYEKATRRGGDMKGYAIIPAPMFEAVQHYIMTSQQ
                     VLELCPTKEGTPGTTLYVAFTAIGYYELPEGSRDAYDVIYITYYGNNDKYKLDAFKDE
                     YIESITIETRSDADMEALTRYLRKYFAPKETAAEYSGSKNGLGLDYEYCYTIKEKAD"
BASE COUNT         1257 a         1020 c          777 g         1486 t
ORIGIN      
        1 aatctgtatt tttcctttat ggcttccctg taagccgata aaatgttttc tcttccagcc
       61 atagcgctta caagcattat aagggtagat tccggaagat gaaagtttgt tataagataa
      121 tcgacaactt taaatctgta tccgggataa ataaagatat cggtttcccc ttcactctca
      181 tgtaaaaccc cgaaatcatc ggaagctcct tccagcacac gaaggcttgt tgtaccgacg
      241 gctattactt ttcgtccttc ccttttagcc gcatttatac tttccgccgc actcctgtca
      301 attctataat actcactgtg cattatatgc tcttcgatat tttccgcttt taccggacga
      361 aaggttccca tacccacatg aagagtcact cgcacaatgt ccacaccttt ttttactaaa
      421 gaatccagca actcctctgt aaaatgcagt cccgcggtag gtgccgcgga agagccgtca
      481 tacttagcat ataccgtctg gtatctgttt ttatcttcca gcttctcggt tatatacgga
      541 ggtagcggaa ccagacctac cttatccaga acctcttcaa atatgccgtc atattcaaaa
      601 cgtactattc tgtttcctaa attaattaca tccagcactt ctgctgcaat taagccgttg
      661 ccgaaaacaa aacgtttacc cttcgctgct ctcttgcccg gtcgtgtcat aacctcccaa
      721 tcggtttttg ttaatctttt taaaagaagc agctctattt ttccgacaga gttctccaca
      781 tgacctaaaa gacgtgccgg aataaccctg gtctcattaa gcaccagaca atcgccttta
      841 tttaaaagat tgacaatatc cgaaaaaacc gtgtgcctta tttccccgct gtttttatct
      901 aaaagaagca atcgtgagtt gtcccttttt tccaaaggat gctgggcaat cagttgctga
      961 ggcaggtcat aataaaagtc gcttttcttc atgctacctc aatgttaccg attccagttt
     1021 aacaccggta tagaagtatc tgataatctc gtcatatgta taaccggcat ccgccatgtt
     1081 tattgctccc cactgtgcca ttcccacacc gtgaccggag cctcttccgg atatggtaat
     1141 cttatcatta ctgtcttcgg taaccaatga aacggttact ataccggagt tggttctgac
     1201 acctatcgtt ttggaagata taagtgatgt attgtatatc ccccttatac ttgcataatc
     1261 caaagcctgt gttgtggcaa caccttttgc atcggcaacc ttaactgtgg actgacctcc
     1321 caatacaaac atctgactgg gcagaaaatc gggaaatgcg ttacgtatgt tggattttaa
     1381 gaaagtaacc gagttattgg atccgtgaac ggtcatggac agtactcttc cggtatgcga
     1441 actggaatca atggtaatct taagaacatt acctattctg tatcccagtt tatacatatg
     1501 gtcggtcatc tgtgatgtgg tataggaata tgtccatgtc ctgtttatgt catacttatc
     1561 cactaccgct ttcagataag gaacggacgt tacccagaca ttctcggaat tttccgtata
     1621 tcctcccgaa gagctgaaat agtagggagt aactacctgc ccggcatatg taactacctg
     1681 tccgtatgtt tcgtttgtag cctgatttgt acgatagttt tccgcattgt acccgccgta
     1741 aacctggcaa cagactttgt cacaaacatc aaaaccgtat tcctgatgtt tgttctgaca
     1801 taaagcataa gtccttgcca ccaccgcctg agctttcagc gcttccatag gcgaattgct
     1861 tcctatctcc aatgccacaa ctccgtaaac ataatactcc agaagcagat tgttcaatac
     1921 gctcaattca ttggacttga aaatggtaaa ttccagattg cctctgtaag gctttccttc
     1981 aatatacata tatgagttgg aatcggtagc aattgttttg gcaattatgg gggccgaagg
     2041 attatcgcag gcaaacagta ttatgctgga tgctttatcc cttaccaata ttcttgcact
     2101 ggaaggatat gctacagtgg aataactgcc gggatacttt gatgcaacac ctgctatacc
     2161 gttcatagcc gattcggatg aagcataact tcctatccac acctgccaga aacccgatgc
     2221 ggcatatacg gggaaagaag ttgcacctgc cgatgacata ttgtttgaaa cattcagagc
     2281 ttcggcatag gaattatagt tgtcatcacg gataacatga taggaataat ccccagtgtc
     2341 tttggctacc acaagcgtat tattagtaga aacaaagtca atttccctgt tatttatgtt
     2401 aatagtcata ttcaatccga acggactgga tacggtatat gcgctttttg ccgtattacc
     2461 gtaatacaaa cccacctgaa gatacaaagg agcgtatata acggcattgg cggtatttac
     2521 gcaagaaaaa cacataaggc atattattgc cagtaaaatc gaaatacttt gtcttttctt
     2581 tctgttcatg agataccctt tcataaaatg accatatcat tagtattatc ttaattatat
     2641 taaaaacaaa actaaataca agtaaaagat gctttgttta tatcagtatt cacttaatcc
     2701 gccttctcct tgattgtata gcagtattca taatccagcc ccaatccgtt tttagacccg
     2761 gaatactcgg cagcagtctc tttaggtgca aaatattttc tcaaatatct tgtcaaagct
     2821 tccatatccg catcgcttct ggtttctatg gtaatagatt ctatgtattc atctttgaaa
     2881 gcatccaatt tatacttatc gttattgccg taatatgtta tgtaaatcac atcatacgca
     2941 tctcgcgagc cctctggcaa ctcataataa cctattgccg taaatgctac atatagagtt
     3001 gtgccggggg tgccttcttt tgtcgggcat aactcgagaa cttgttggga tgtcattata
     3061 taatgctgaa cggcttcaaa catgggtgca ggtattatgg catatccttt catatcgccc
     3121 cctcttcttg tcgctttttc gtagtaatcc tgctccctct gcttaacaag atctctattt
     3181 ctgtaattac cgtaaaaata tatctcacaa ccctgtttat ctctttttat ctcacttatg
     3241 cttgtaacgg ccttatgata aggcggtaca ttaaaactca tccagactct gtcttgttgc
     3301 attccgagag gcatcaattt taaaccgtaa ggctggtatt ttgttcccgt cgggtaatct
     3361 gcgggattaa caccttttat acccgtacct atattaatat cgcttatata ctgcgaatac
     3421 tcatggtatt ctttaaccaa tgcatcgcca tataaatgtg acatcataag atcggatttc
     3481 tcatcgattc tggaaacggt tatgatattc tttttgaagt ttgtatcttc ttcaataagt
     3541 accggcttta tacccgcagt gtttacaaac atatacggat agcgttcatt aaacctgttt
     3601 atcctctttt catattgaga aagcacatta tagtccgcaa aataattgtt taatacactt
     3661 ataagaccgg aaaggtcttt tccctcattg acatctatct ccatgcagtc aatataatcc
     3721 ggtatatcta tctgggctcc cttcgctact ttacccattc cataaaagga aaggtacaaa
     3781 gcatcatgtt ctttatttgt cgtatactcc cctattatct taaaaggttc tgcgcccctt
     3841 gtttcgttat tttttacata gaaaacctta tcggagtcag ataactcctg attaatgtgt
     3901 ttactcaagg tagaggggat aatagcatag ttttgagacg tctcaagtag caactcttca
     3961 ttatagcctt cataaattgt tattttacta tcatttttgt ctgcaattat accgcttaat
     4021 gtagttatgc ctttataata atctattgaa tttatgactc tcgtaacttc actgctgtcc
     4081 ggttctacta agattttttc ataaccggac atacttaacc ctttcctgcc tatcttacat
     4141 cttacagaag agatattgat accgattact tcttccgata atcccgctct tattatgggc
     4201 atagcatatc tgtacggcat attcaacggc tcctgcccgt ccactctttt tattactatg
     4261 tcactgttaa aggtagttat attaaaacct tccaaagttt cctgaggctt acaagatacc
     4321 gataacaatg ctatgacaat aaccgtcaga atggctatta cagtcatacc tgtttttctg
     4381 ttatttttca tgttgctaac ctccaagatt ttctgcaaaa tttatgtaga acaaggtcgg
     4441 attgtttaag caaaacattt cttttacttc tttgataaag tttttgtata tgtttccact
     4501 gcatttacta ttttatcaca aaaagatatg tgaaagaaga
//
LOCUS       DAOSOM010000078         3999 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_22671_length_3999_cov_5.051978, whole genome shotgun sequence.
ACCESSION   DAOSOM010000078 DAOSOM010000000
VERSION     DAOSOM010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22671_length_3999_cov_5.051978"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1850
                     /locus_tag="PLF14_09855"
     CDS             <1..1850
                     /locus_tag="PLF14_09855"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOT71624.1"
                     /translation="DAAILVSRVIDRPMRPLFPHDLRNDVVIICTALSVEQDNQPQLA
                     AMLGASIATSISDIPFDGPVASVALGLVDGKVVLNPDAAQRAVSDMYVTVAGTKEKIC
                     MIEAEAKQVPDDVMFNALLIGHEEIKSVCAQIEKIAAEIGKEKFTYESAAVPKEVYAE
                     VEDIALDEMKQAVLSADKTVREAKVDQLTATVNEQLGEKYADNPGYIADALYNLEKKV
                     VRHYLLHEKKRVDGRALDEIRPLYSEVGVLPRTHGSAMFQRGQTQVLSSVTLGGLRDV
                     LPLEILDEAETEDRYMHHYNFPSYSVGEAKTSRGPGRREIGHGALAQKALLGVIPSEE
                     EFPYAIRVVSDVLMSNGSTSQGSVCGSTLALMDAGVPIIAPVAGISCGLVTDADNPSN
                     YEMMIDIQGIEDFFGDMDFKVAGTHKGITAIQVDIKIDGLTPEIIKRAFEITRKGRLY
                     ILDEVMLKVIDKPRPELSPYAPKIITMTIDPEKIKDVIGTGGKIINRIIDMTGVKIDI
                     NDDGTVYILTVDQQSGDKAVSIINGIVNDLEPGMIFNDAVVTRIMDFGAFVELVPGKE
                     GLVHISQIAHERINKVSDVLSVGDKVNVKVVEIDRQGRVNLSRKALLPR"
     gene            1894..3138
                     /locus_tag="PLF14_09860"
     CDS             1894..3138
                     /locus_tag="PLF14_09860"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOT71625.1"
                     /translation="MIEIKKLANGTRVIFEHMPVGDIISIGIFVKAGSNYENARNNGI
                     SHFIEHMVFKGTESKSAKDIAIMSDRIGGTLNAYTSKDMTCFFIKTTSDNAATALELI
                     SDILLHPAFEPKTVENEKRVILEEIAMYEDFPEEQVQEQLEKEMWKGNPLSFPIAGSK
                     QTVMSFTEDTVRNHMNKFYVSANTVISVSGCFDKKAMFHMISRYFEDYTSGNCEASDK
                     GVLAQFRTADVIKTKKIEQAYLSMAFEGCVQDSPERYPFSVLSGILGQSMSSRLFQTV
                     REEKALAYNIGSFNASFQTGGILNIYAGCNPERYDDMMHCIQEIIEDISRNGPEDEEI
                     TRARDQIRCSMLLGMETTDSRMISNGRNMLLRGRPVSREEAIEKIDKVTKDDVQSIID
                     KYIDWNRKAVSVLRPSKKTKKS"
     gene            3284..>3999
                     /locus_tag="PLF14_09865"
     CDS             3284..>3999
                     /locus_tag="PLF14_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004128505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT71626.1"
                     /translation="MEGYKKRVADAILEEKLAGKGAVLIEGPKWCGKTTTAEQRAKSI
                     LYMADPVRRERNLMLAETDPDILLRGEVPRLIDEWQIAPKLWDAIRFEVDRRKAFSQF
                     ILTGSSVPADTKDITHSGTGRFSYLRMRPMSLSESGESDGSISLRELFSSPDNIYAGC
                     DLTLSDICFLICRGGWPLSTYLSGKLALNQARDYYDAVVNVDMSRVDNVERNPERVKR
                     LMRSYARHQGQRVADTAICD"
BASE COUNT         1267 a          726 c         1021 g          985 t
ORIGIN      
        1 ccgatgcggc aattttagtt tccagagtaa tagacagacc tatgagaccg ctttttcctc
       61 atgatttaag gaatgatgtg gtcattatct gtaccgctct ttctgtggaa caggataatc
      121 agcctcagtt ggctgccatg ctgggagctt caatagccac aagcatttcc gatatccctt
      181 ttgacggacc ggtagcgtcg gtagctctgg gactggtcga cggtaaagtg gtattaaatc
      241 ccgatgccgc acaaagagcg gtatcggata tgtatgtcac agttgcggga acaaaagaaa
      301 agatatgcat gattgaggcg gaagcaaaac aggtacccga tgatgtaatg ttcaatgctt
      361 tactgatcgg acatgaggaa ataaagtcgg tttgtgctca gattgaaaaa atagccgcgg
      421 aaataggaaa agaaaagttt acctatgaat cggctgcggt accgaaagag gtgtatgcgg
      481 aagttgaaga tatcgctctt gatgaaatga aacaggcggt tttatcggca gataaaacgg
      541 taagagaagc caaagtggac caacttacag ccacggttaa tgagcagtta ggtgagaaat
      601 atgccgataa tccgggatat atagccgatg cactgtacaa tctggaaaag aaagtggtaa
      661 ggcactatct tcttcatgaa aagaaaagag tggacggcag ggcattggac gaaataaggc
      721 ctttatacag tgaggtgggt gtgcttccca gaactcacgg atcggctatg ttccagagag
      781 gacagactca ggtgctgtca agtgttaccc tcggaggatt gagagatgtg cttccgttgg
      841 aaatattgga tgaagcggaa accgaagaca gatatatgca tcactacaat ttcccgtcat
      901 actctgtggg agaggcaaag acatccagag gaccgggcag aagagaaata ggtcacggag
      961 cgcttgctca gaaggctctt ttaggagtta ttccttcgga ggaagagttc ccgtatgcta
     1021 ttagagtggt gtccgatgta ttgatgtcca acggttccac aagtcaggga tcggtctgcg
     1081 gaagcacatt ggcattaatg gatgccggtg ttcccattat cgctccggta gcgggtattt
     1141 cctgcggatt ggtcactgat gcagacaatc cttccaatta tgaaatgatg atagatattc
     1201 agggtattga agatttcttc ggagatatgg acttcaaggt agcgggaacg cataaaggta
     1261 ttaccgccat acaggtggat ataaagattg acgggcttac accggagatt ataaaaagag
     1321 cgtttgagat tacacgtaag ggcagacttt atattttgga tgaagtaatg ctgaaagtta
     1381 tcgacaaacc cagaccggaa ctctccccgt atgctcccaa gataataacc atgacaatag
     1441 atcccgagaa gataaaggat gtcataggaa cgggcggaaa gataatcaac aggataatcg
     1501 acatgaccgg agtcaaaatt gacatcaacg atgacggaac ggtatatatc ctcaccgtag
     1561 accagcaatc gggagacaag gccgtttcca ttataaacgg tatagtaaat gatctggaac
     1621 cgggcatgat attcaatgat gccgttgtta caaggattat ggacttcgga gcttttgtgg
     1681 aactggtgcc gggaaaagaa gggctggtac acatatcaca gattgcacac gagcgtataa
     1741 acaaagtaag tgatgtattg agtgtaggag acaaggtaaa tgtcaaagtc gtggaaattg
     1801 acagacaggg aagggtaaac ctttcaagaa aagctttgtt gcccagataa aacaaacgcc
     1861 cgggtaacac cgggctttct ttttagagag aaaatgatag aaataaagaa attagcaaac
     1921 ggtacaagag taatattcga acacatgcct gtaggcgata taatcagcat aggcatattt
     1981 gttaaggcgg ggagcaatta cgagaatgcc cgaaataacg gaatttcgca ctttatagag
     2041 catatggttt ttaaaggcac ggaaagcaaa agcgcaaaag acatagcaat catgtccgac
     2101 cggataggcg gaacgttaaa tgcatataca agtaaggata tgacatgttt tttcataaaa
     2161 accacatcgg acaatgccgc caccgccttg gagcttatat cggatatact tttacatccc
     2221 gcttttgaac ctaaaaccgt ggaaaatgaa aagcgggtta tcttggaaga aatcgccatg
     2281 tatgaggatt ttcccgaaga acaggttcag gagcaattgg aaaaagaaat gtggaaaggc
     2341 aatcctctgt cctttcccat tgccgggtcc aagcaaacgg taatgagttt taccgaagat
     2401 actgtaagaa accatatgaa taagttttac gtttctgcca atacggttat ttcggtatcc
     2461 ggttgctttg ataaaaaagc tatgtttcat atgatatccc gatactttga agattacaca
     2521 agcggtaact gtgaggcatc cgataaaggt gttttggcgc aatttcgaac ggcggatgta
     2581 attaaaacca agaagataga gcaggcatac ctttccatgg catttgaagg atgtgtgcag
     2641 gattcgccgg aacgctatcc tttttccgta ctttccggca ttttggggca gagcatgtct
     2701 tcaagactct ttcagacagt acgggaagaa aaagctttgg catacaatat cggatcattc
     2761 aatgcctctt ttcagacagg cggcatactg aatatatatg cggggtgcaa tcccgaacga
     2821 tatgatgata tgatgcattg tattcaggaa ataattgaag atatatcccg aaacggtccg
     2881 gaggatgagg aaataacaag agcccgcgac cagataaggt gttccatgct tttaggaatg
     2941 gaaactaccg attccagaat gatttccaac ggacggaata tgttgctgag gggtcgaccg
     3001 gtatccaggg aagaagcaat agaaaaaatt gacaaagtaa caaaagacga tgttcagagt
     3061 attatagaca agtatattga ctggaaccga aaggcggtat cggtcttacg tccctcaaag
     3121 aaaacaaaaa agagttgatg ttactctaat cgccaataat gggaacgaaa atgcgccaat
     3181 aagaggaatg aaagtgcgcc aataagagga aaagcgttga cagacaggta attattggtt
     3241 atcataccta tataattact gtttttatga ggcgatatat ataatggaag gatacaagaa
     3301 aagggtagcg gatgctattt tagaagaaaa actggccggt aaaggtgcag tactcataga
     3361 ggggcctaag tggtgcggta agactacgac agccgagcag agagcaaaga gcattctgta
     3421 tatggccgat ccggtaagac gggaaagaaa tcttatgctt gctgaaaccg atcccgatat
     3481 acttttgagg ggggaggtgc ccagattaat tgacgagtgg cagattgctc ctaaactttg
     3541 ggatgcaata agatttgagg tggatagacg taaagcattc tctcaattca ttcttaccgg
     3601 ttcatcggtt ccggcagata caaaagatat aactcactcg ggaacaggca gattctccta
     3661 tttgcgcatg agacctatga gtctttcgga atcgggcgaa tccgacggtt caataagtct
     3721 tagagaactg ttttcatcgc ctgataatat atatgccgga tgcgatctga cattgagtga
     3781 tatatgtttt cttatatgcc gcgggggatg gcctctttca acttatctgt caggaaagct
     3841 tgccttgaat caggcaagag actattacga tgcggtcgtc aatgttgata tgtcaagagt
     3901 ggataatgtg gaaagaaatc ccgaaagagt gaaacggctt atgagatcgt atgcccgtca
     3961 tcagggacaa cgggttgcgg atactgctat atgtgatga
//
LOCUS       DAOSOM010000079         3794 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_24230_length_3794_cov_5.175983, whole genome shotgun sequence.
ACCESSION   DAOSOM010000079 DAOSOM010000000
VERSION     DAOSOM010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24230_length_3794_cov_5.175983"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /gene="obgE"
                     /locus_tag="PLF14_09870"
     CDS             complement(<1..799)
                     /gene="obgE"
                     /locus_tag="PLF14_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007050616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOT71627.1"
                     /translation="MFIDEVTIYVKAGKGGNGAATFRREKYEPAGGPDGGDGGKGGDI
                     VFVADNNVRTLLDVKYNRKYYAKDGEKGMRKNMTGKSGENTVIRVPVGTLITDTDRNK
                     LICDLNKDQMSFTVCEGGRGGRGNTRFATPTRQAPEFSEAGMEGEEKNLRLELKLLAD
                     VGIIGMPSVGKSTLISVITSAKPKIADYHFTTLVPNLGVVRLNDETQFVVADIPGLIE
                     GASEGAGLGHAFLRHIERTKLLVHMIDASELEGRDAVKDFDTINNELA"
     gene            complement(837..1094)
                     /gene="rpmA"
                     /locus_tag="PLF14_09875"
     CDS             complement(837..1094)
                     /gene="rpmA"
                     /locus_tag="PLF14_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOT71628.1"
                     /translation="MAHKKGVGSTKNGRDSQSKRLGVKRGDGQAVLAGTILVRQRGTK
                     IHPGENVGIGKDDTLYAKANGTVSFSRMGRDRKKVSIVVAQ"
     gene            complement(1113..1421)
                     /gene="rplU"
                     /locus_tag="PLF14_09880"
     CDS             complement(1113..1421)
                     /gene="rplU"
                     /locus_tag="PLF14_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016146398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOT71629.1"
                     /translation="MYAIIETGGKQYKVSEGDTLYIEKIEAEEGKVKFDKVLAVATDE
                     GLKVGKPYVEKATVTAEVEKTGKAKKILVFKYKAKKGYKKMQGHRQPYTKVKITKISL
                     "
     gene            complement(1826..2950)
                     /locus_tag="PLF14_09885"
     CDS             complement(1826..2950)
                     /locus_tag="PLF14_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71630.1"
                     /translation="MTKKVSALIMLILVMACLVSCNTGTVREPVQSSVLKPETYYTSF
                     KISACFGRFIETPYGSYFASGNRYIYYSHKGNTKYVKLCNKPDCSHNSEDCNAFLKGS
                     VIGYYNNKIYYRRFKHIYCMDMDGCNHKIVKALYEEHCSNHGYFHNGYYYYIKTNFAE
                     YNGVKGKDDPTLYRTKVDDKSESEVLLTNDLILQTDMFTMIGDNIYISVRNSDPDKIS
                     SLYSYSTVTKSLSKLTDDWSGLGSYLKDENKGYCYVKNKGFYEYDFGTNEMSLVKPIE
                     LENGGNSSAFYLDDYILLVHNNSVPFDYENQVMCIYDRDYNLLNTIVFDKANGKIPGG
                     FLSDVDGYIIFSSNFRDKPDYYIDKSEIGTDNLQFHKIED"
     gene            complement(2962..>3794)
                     /locus_tag="PLF14_09890"
     CDS             complement(2962..>3794)
                     /locus_tag="PLF14_09890"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HOT71631.1"
                     /translation="EPVPSSSINLNEIEIRNMTSLKIYLESILKSEQGKHGLSLYNTV
                     TEEYIGINDTELFVAASSTKVPMVMYVYSAVEKGQLSFDDTVVYTESDYEVGTGIIIK
                     GEFGDVYTIGELAEYAIIHSDNCAINMLIRTCNEWYMVNYMNKLGAKVDYIKKRWKTC
                     PYDLTLYYKELLRLVEENPEHYQKLMDDLCSNKVGYWMSSKSVLPEDLQIAMKTGINT
                     ILPTYNEATIVFTKHPYILSYCSTDIVIEESTELHKKISKVIYDFMENGYAVGELKWK
                     "
BASE COUNT          992 a          863 c          635 g         1304 t
ORIGIN      
        1 tagccagctc attgttgata gtatcaaaat cctttaccgc atctcgccct tcaagttccg
       61 atgcatctat catatgaacc aataattttg ttctctcgat atgtcgcaaa aatgcatgtc
      121 ccaagcctgc tccttccgat gctccttcaa ttaaaccggg aatatcggca acaacaaact
      181 gtgtttcatc atttaatcgc actactccga gattgggtac caatgtggtg aaatgataat
      241 cggcaatttt gggttttgcg gatgttataa ccgagattaa ggtggattta cctaccgaag
      301 gcataccgat tatacccaca tcggctaaaa gctttaattc cagacgaagg tttttctctt
      361 ccccttccat gccggcttcg gaaaactcgg gagcctgtct tgtaggtgtg gcaaaacgtg
      421 tatttcccct tccccctctt ccgccttcac atactgtgaa agacatctga tctttgttta
      481 aatcgcatat aagcttattc ctgtcggtat cggttataag ggtgcctacg ggaactctta
      541 tgacggtatt ttctccggat tttcccgtca tgtttttccg cattcctttt tcaccgtctt
      601 ttgcgtaata ctttctgttg tattttacat ccagcaaggt acgcacatta ttatcggcaa
      661 caaaaacaat atcgccgcct tttccgccgt ctcctccgtc cggtcctcct gccggttcgt
      721 atttttctct tctgaaagta gcggctccgt ttccgccttt acctgctttt acgtatatcg
      781 ttacttcgtc aataaacata atcaaaaaaa ccgcctcggt tggaggcggg tgttttttac
      841 tgggctacga cgatactgac cttttttctg tctcttccca ttcttgagaa actgacagtt
      901 ccgtttgctt ttgcatataa cgtatcatct ttgccgattc cgacattttc accggggtga
      961 atcttggttc ctctttgtct tacaagaatt gttcctgcaa ggaccgcttg accgtcacct
     1021 cttttaacgc ctaatctttt actctgactg tctcttccgt tctttgtact tccaacacct
     1081 ttcttatgtg ccaatttaat tcacctccga tcctataaag aaattttagt aatttttact
     1141 ttcgtgtaag gttgtctgtg tccctgcatc tttttgtatc cctttttggc cttatactta
     1201 aacacaagta ttttctttgc cttaccggtc ttttccactt cagcagttac tgttgctttt
     1261 tcaacataag gttttcccac cttaagtcct tcatctgtgg ccactgcaag aaccttgtca
     1321 aacttaactt ttccttcttc cgcctctatc ttctcgatat agagtgtgtc gccttcggat
     1381 accttgtatt gttttcctcc ggtttcaata attgcgtaca tcctttgttg cctcctaagc
     1441 aaaatctcgc cttgccaagg taagtaattg catactttta aaacccctca agtgcggcta
     1501 ccgacagatt ctatcataag gaggtatggg tgtcaataac gagtttttcc cgtgttttcg
     1561 ccttttaaat aacttatatc tcgttttatt aagggaagca taaacgtcgc aacgccttct
     1621 gctccccttt gctaactgat ttttccgctg tccgccagcc actggcacat acgtccagca
     1681 agaatgcaag ttttaatgtt tcttgccaac atccggtaca ttgtccggca aattcggatt
     1741 ttaggtgtca gtccaccgca acttcacatt aaacaatatt cccaaaaatg tcaagaaaca
     1801 aatatatttt ttacattaac tttaatcagt cttcaatctt atgaaactgc aaattatccg
     1861 ttcctatctc ggatttgtct atgtaatagt cgggtttatc acggaagttg gaagaaaata
     1921 ttatataccc gtccacatca cttaagaatc cgcccggtat tttgccgttt gctttatcaa
     1981 atacaatggt atttaagaga ttgtaatccc ggtcataaat gcacataacc tgattctcat
     2041 aatcaaacgg aacactgtta ttgtgaacaa gaagtatgta atcatctaaa taaaaagcac
     2101 tgctattgcc accgttttcc agctctatgg gcttaacaag gctcatttcg tttgtgccga
     2161 aatcatattc ataaaatccc ttatttttga cataacaata acctttattc tcatctttta
     2221 aatatgaacc taaaccggac caatcatcgg tcagttttga gagacttttt gtaactgtcg
     2281 aataagaata taacgaagat attttatcag gatcggaatt ccgcacgctt atgtatatat
     2341 tatctccgat catcgtaaac atatccgttt gcagtatcaa atcatttgtc aataatactt
     2401 cggactccga tttgtcatct acttttgttc tgtaaagagt agggtcatct tttcctttta
     2461 ctccgttata ttctgcgaaa ttagttttaa tgtagtaata atatccgtta tgaaaatatc
     2521 cgtgattact acaatgctct tcatacaatg ccttaactat tttatgattg catccgtcca
     2581 tgtccatgca atatatatgt ttgaaacgcc tgtagtaaat tttattatta taataaccaa
     2641 tcacactccc ttttaaaaac gcattacaat cttccgaatt atggctgcag tcaggcttgt
     2701 tgcacagttt aacatatttc gtgtttccct tatgggagta atatatatat ctgtttccgg
     2761 aagcgaaata cgaaccgtag ggagtttcaa taaatctacc gaaacaggcg cttattttaa
     2821 acgaagtata atatgtctcg ggctttaaaa cagaagattg gacaggttcc cggacggttc
     2881 cggtattaca cgataccaga caagccataa caagtatgag cattatgagg gcagaaacct
     2941 tttttgtcat acgattacct gctatttcca ctttaattca cctacagcat atccgttttc
     3001 catgaaatcg tatataacct tgcttatctt tttatgaagc tctgtagatt cttcaattac
     3061 tatatcggtc gaacagtaag ataagatata agggtgtttt gtaaaaacga ttgtagcttc
     3121 gttatatgta ggtaatatgg tatttatccc ggttttcatg gcaatctgca agtcttcagg
     3181 cagtaccgac ttggaagaca tccaataacc taccttgtta ctgcaaagat cgtccatcag
     3241 tttttggtag tgctcggggt tttcttccac aagccttaat aattctttgt agtaaagcgt
     3301 aagatcataa ggacaggttt tccagcgctt tttgatgtaa tccaccttgg cacctaattt
     3361 gttcatgtaa ttgaccatat accattcatt acaggtccga ataagcatat ttatcgcaca
     3421 gttgtcactg tgtataatgg catattccgc caattcccct atcgtatata catctccgaa
     3481 ctcccctttg attattatcc ccgttcccac ttcataatca ctttcggtat aaaccacggt
     3541 atcatcaaag gaaagttgcc ccttttccac tgcgctataa acatacatta ccataggcac
     3601 ttttgtggat gatgccgcta caaacagttc ggtgtcgtta atgcctatgt attcctccgt
     3661 aaccgtgttg taaagactta atccgtgctt accttgctcg gatttaagaa tcgactcaag
     3721 gtaaatcttg agtgaagtca tgtttcgtat ttcaatttcg tttaagttta tacttgatga
     3781 cggtacaggt tcag
//
LOCUS       DAOSOM010000080         3623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_25715_length_3623_cov_5.217769, whole genome shotgun sequence.
ACCESSION   DAOSOM010000080 DAOSOM010000000
VERSION     DAOSOM010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25715_length_3623_cov_5.217769"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="PLF14_09895"
     CDS             complement(<1..863)
                     /locus_tag="PLF14_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014041755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT71632.1"
                     /translation="MIEIKNLVKHYGQVVAVDDISFKVKEGEILGFLGPNGSGKSTTM
                     NIITGYLPSTSGTVEVAGYDITTHPEEVKKRIGYLPEQPPLYKDMTVTEYLSFVSDLK
                     KAPKSKKKSELSDIMELVGLTDHRKRLIDNLSKGYRQRVGLAQALVGNPKVLILDEPT
                     VGLDPKQIVEIRRVIKGLATDRTIILSTHILPEVSMICERVVIINKGKIVAEDTPDRL
                     SASLSKIAKLNLTVKGDKDKVLKTLGNIPNVRYVTMTTKAEDSVFKYVLETGSDKDIR
                     EEIFYKMAEIKA"
     gene            1032..1421
                     /locus_tag="PLF14_09900"
     CDS             1032..1421
                     /locus_tag="PLF14_09900"
                     /inference="COORDINATES: protein motif:HMM:NF025384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4234 domain-containing protein"
                     /protein_id="HOT71633.1"
                     /translation="MYDGEHRPLILIILFSIISVGFFNFYWLYIATYEIKQVSREADR
                     IKPWLETFLAVITAGFYIIYWSYKYNKLLYDAQAVHKVTPQDVALPAAIFSFPVITFI
                     GMCFTQHQLNRLWKYGTAPQAKKKKDK"
     gene            complement(1469..1813)
                     /gene="rnpB"
                     /locus_tag="PLF14_09905"
     ncRNA           complement(1469..1813)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLF14_09905"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1897..2640)
                     /locus_tag="PLF14_09910"
     CDS             complement(1897..2640)
                     /locus_tag="PLF14_09910"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOT71634.1"
                     /translation="MRISKIIKYLEYVAPTDKSTERDNVGLLIKGKDNIRRVMVCLDI
                     TKDAAEYAVSCKADLIITHHPFIYDPLYRIDNDELIAVIRNKSAVISLHTNYDAARLN
                     DVLAEKCFLREIKHIFFEEGISLGREGITDTTGFNEYIEKIKKSLGVDIVKVTGNIPD
                     KVSAVAVGTGSSGSFAEDIKELDVDVFVTGEIKYDIIKSIAGTGPAIVELGHYESEEV
                     FVDDLSALLAEKFPSLDIFPYKRRISAYF"
     gene            complement(2637..3314)
                     /locus_tag="PLF14_09915"
     CDS             complement(2637..3314)
                     /locus_tag="PLF14_09915"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT71635.1"
                     /translation="MREVKRLLSNRLLMVADLAVPCEKVFDIGTDHCYLPIYLIQKGI
                     CKTAVATDIKEGPVRIAKRNVRLFGMEKHIDVFVADGISHVEEGCCVIIAGMGADVII
                     DILKKDMDFAKSCEWLIVQCQSKTECLRAFLWDNGFAIEEEALINDRDKIYNAFRASY
                     TGINRPYTETDTIASEILAKRHHPLLPGYVSGYIKKLDDMIAGYREAGRDFTEEIKLK
                     RALEELL"
     gene            3504..3580
                     /locus_tag="PLF14_09920"
     tRNA            3504..3580
                     /locus_tag="PLF14_09920"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3538..3540,aa:Ile,seq:cat)
BASE COUNT          943 a          856 c          612 g         1212 t
ORIGIN      
        1 gcctttattt ccgccatttt gtaaaatatt tcttctctga tatctttatc gcttccggtt
       61 tccagaacat atttgaatac cgaatcttcg gctttggtag tcatggttac atacctgaca
      121 ttgggtatat tacccaaagt ctttaacacc ttgtctttgt ctccttttac cgtcagattg
      181 agtttggcaa tctttgaaag acttgcggaa agtctgtcgg gagtatcctc tgcaacaatc
      241 tttcctttgt ttataattac taccctttcg catatcatac ttacttccgg taatatgtgc
      301 gtactcaaaa taatggttct gtcggttgcc agccctttta taactcttct tatctccact
      361 atctgcttgg ggtccagtcc caccgtaggc tcgtccagaa tcaatacctt gggatttccc
      421 accagtgcct gtgccaatcc caccctttgc cgatatcctt tggacaagtt atcgataagt
      481 cgctttctgt ggtcggtcaa tcccactaac tccattatgt cggacaattc gctttttttc
      541 ttacttttcg gagctttttt caagtcggaa acaaaactca gatattcggt tacggtcata
      601 tctttatata gagggggctg ctcgggcaga tagcctattc gcttctttac ctcttcggga
      661 tgagtggtaa tatcataccc ggccacttcc actgtcccgc ttgtcgacgg aagataaccc
      721 gttatgatat tcattgtggt ggacttaccg gaaccgttgg gtcccaaaaa ccccagtatt
      781 tccccttctt ttactttgaa actgatatca tcgacggcaa caacctgtcc gtaatgtttg
      841 acaagatttt ttatctcaat cactgggctg acctccttat tatttagtct ggcatcagtt
      901 ttacagcctt tatattataa cgcattatat actcttttca acattttatt aagtatttgc
      961 attattcgtt agttctaatt actttaatta atgttaatat atatgatatc atgtattcaa
     1021 cggaggcttt tatgtacgat ggtgaacaca gaccgctgat acttataata ttgttttcca
     1081 taatatcggt gggtttcttt aatttttact ggctatatat tgcaacatat gaaatcaagc
     1141 aggtttcgag ggaagcggac agaataaagc cgtggctgga aacattctta gctgtaataa
     1201 ccgccggctt ctatataatc tactggtcat ataaatacaa caaactttta tacgatgcac
     1261 aggcagtaca taaagttacc cctcaggatg tagctcttcc cgcagcaata ttctctttcc
     1321 ccgttattac atttatcgga atgtgcttta cgcagcatca gctcaacaga ctgtggaagt
     1381 acggtaccgc tccccaggcc aagaagaaga aagataaata atattcaata atcccggata
     1441 ttaaaaaatg tgctgtatta gcacattttt ccttaccgga tctgtaagcc gggttctgta
     1501 ttagacgatc atctgtctag gcacaatatc tctattatac tcaagccacc tttagagacg
     1561 ggcgggcaac ccatagtccc tttttgcggt gttgctccgg atggggttta catagcctga
     1621 tatgttacca tatcgctggt gagctcttac ctcgcctttc cacccttacc gtattgctac
     1681 ggcggtatct ctctgttgca ctttccttga agtcaccttc accggtaatt agccggcatc
     1741 ctgcccttcg gagcccggac tttcctcatc tgcagccttt aggcattgca tccgcgatcg
     1801 tctgatccgg tcagggcagt aattatatca ctttattata tttttatcaa attatatgtt
     1861 tttatcacct ttaaaatact ttacactgaa ttttaattaa aaatatgctg aaattcttct
     1921 tttgtacggg aaaatatcca atgaaggaaa cttttcggcc aaaagggcgg ataaatcatc
     1981 aacgaaaacc tcctccgatt catagtgtcc caattccacg attgcaggtc ccgtacccgc
     2041 tatggatttg ataatatcat actttatctc tcccgttaca aacacatcga catccaattc
     2101 ctttatatct tccgcaaagc tgcctgaaga accggttccc acagccactg cacttacctt
     2161 atccggtata ttgccggtca ccttaacgat gtccacaccc aaacttttct ttatcttttc
     2221 gatgtattca ttaaagcctg ttgtatcggt gattccttcc cttcccaaag atattccttc
     2281 ttcaaaaaag atatgtttta tttctcttaa aaagcatttt tccgccaaaa catcattcaa
     2341 ccttgccgca tcataattgg tatgaagcga aatcacggca cttttgttgc gtataacggc
     2401 aataagctca tcgttatcta tcctgtaaag aggatcataa atgaagggat gatgagttat
     2461 tattaaatcg gctttacagg ataccgcata ttccgcggca tcttttgtaa tatccagaca
     2521 taccataacc cttcttatat tgtcctttcc cttgattagt aacccgacat tatcccgctc
     2581 tgtactttta tccgtaggtg caacatattc cagatattta ataatttttg atattctcat
     2641 aacagctcct ccaatgctct ttttaatttt atttcttcgg taaaatctct gcctgcttcc
     2701 ctgtatcccg ctatcatatc gtccagcttt tttatatacc ccgaaacata tcctggtaac
     2761 agaggatgat gtctttttgc taaaatttcc gatgctatcg tgtcggtttc ggtgtacggc
     2821 cgattaatac ccgtgtaact tgcccgaaaa gcattatata tcttgtctct atcattaatg
     2881 agtgcttcct cttcaatagc aaatccgtta tcccacaaaa aggcccgcaa acattcggtt
     2941 ttgctctgac actgtacaat caaccattca caactttttg caaagtccat atctttttta
     3001 agtatatcga ttatgacatc ggcacccatt ccggctataa taacacaaca gccttcctcc
     3061 acatgactta ttccgtcagc gacaaacaca tcgatatgct tttccatacc gaacagccgg
     3121 acatttctct tggcgattct taccggtccc tcttttatat cggtggcaac ggctgtttta
     3181 catatacctt tttgtatgag atatatgggc agatagcagt gatcggtacc tatatcaaat
     3241 accttctcac acggcaccgc caaatccgct accatgagca atctgttgct taacagtcgt
     3301 ttaacctctc tcatattccc tattttacat tatcatcggt atatttaata ttataatcgc
     3361 ggtttttata ctcaaaatac gatttctccg tatttttaaa tgaaatttga ccttaaaagg
     3421 acttaaggga gcaacgaaat cttgaaaaaa gttgttgacc gggtttatac ttgtatgtat
     3481 aatatatcaa gtattgagct ttagggcctt tagctcagtt ggttagagca accggctcat
     3541 aaccggtcgg tccgcggttc gagtccgtga aggcccacca ccgataacaa atccgaacat
     3601 atttccgttg tgtaatgcgt tcg
//
LOCUS       DAOSOM010000081         3564 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_26294_length_3564_cov_4.938444, whole genome shotgun sequence.
ACCESSION   DAOSOM010000081 DAOSOM010000000
VERSION     DAOSOM010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26294_length_3564_cov_4.938444"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..1771
                     /locus_tag="PLF14_09925"
     CDS             101..1771
                     /locus_tag="PLF14_09925"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOT71636.1"
                     /translation="MNNYIVKCVSSLEKCFLDDNIQDKKQYTSFSCLLNEKLNFQIAY
                     TMTSDTLKDKSPCYFHIKSELKEFIRVYKVESVAVRLPVYHNLPTDNYQRKVPHCYPD
                     LLCPYDKKDNVYFVPDLLQSLFIEVDLSNNTCFYGDYVIKCYFTDNDGNTVASTNVSV
                     NIINSMLSDKRITFTQWFHGDCLASYYAVKPLSKKHFAIMKNFLKTAYEYGINAILTP
                     VLTPPLDTKIGGERPTMQLVDITKTSSGYEYSYDLLDKWIDMCNEIGFEYFEISHFFT
                     QWGAKHAPKVVAYVNGRKKRIFGWETDATSDEYISFLRDFISNLLVYLKNKGVDKRCF
                     FHISDEPNEKNIEGYRNARNSIIDLLDGYLVTDALSSIEFYKQGIVTSPIPATDKIEP
                     FLKQNIDNLWIYYCCGQFIDVSNRFIAMPSQRNRIIGTQLYKYRISGFLQWGYNFYYN
                     QYSRKLVNPFLITDGDFFAPAGDAYSVYPGHDGKPLKSLRLVVFNEALQDLRAFLKLE
                     SLTSYEHVMKIIESNGKISFSKFPEDIEYIINLRERVNKEIDKLTEKK"
     gene            1942..>3564
                     /locus_tag="PLF14_09930"
     CDS             1942..>3564
                     /locus_tag="PLF14_09930"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOT71637.1"
                     /translation="MISGLISVTVLFIYTKDKSVAKIIKVLLLSFFVIAFTVFFFFSR
                     LLFMNIENDYSDENRKEVRLTGYVTEIVKDQVNKFKVILKCNNGFNYFVTMKDDYIRI
                     GDIIELSGLLYKPESAGNKGQFDYQKYCYSKNISADIYVNRGNISKIGSKPFIGFIGN
                     IREKAVERVMKHLNEDKRGIVTALIAGSRTYLDRNTTSTFQKSGLSHILSISGTHFNI
                     LIIPFYYLLTLLSKNNRFSMIFTIFAVLFLLVFTGLRISAIRAALCIVTLFFSNMLYF
                     DSNRYLPICLSSLIIILINPLSVYDYGFIMSYCCVVSIALFSRKIRSFFVLQIDKLKK
                     TLQEKVLKKHIVLEDYYRRIRKTKLKAVILKVLSALSLLLSVQPLTVFISYRLFHCIY
                     PYSLFSNLIIFILITPLLLSLWTAILFPRIMCPLFGLLANFLIKFAEYIGNLPYSKLY
                     VKHIPDIFLIIIICTFLYIKYKPFKSRINKYIVISVATLLCVITICINNTPKVVFMDV
                     GQGDCAVITLSNGYNIMIDTGEYIDLSAVAYRT"
BASE COUNT         1189 a          563 c          629 g         1183 t
ORIGIN      
        1 ataatcttat acgcgccaga acgcaattta agctggttaa ggaaatagaa gggtatttac
       61 ccaaaatgaa tgaaatagtg caaaaaatgg gaagtgagct gtgaataatt acattgtaaa
      121 gtgtgtatca tcattggaga aatgtttttt ggacgataat atacaagaca agaagcaata
      181 tacttcattt tcctgtttac tgaatgaaaa acttaatttc cagattgcat atactatgac
      241 aagcgatact ttaaaggata aaagcccctg ttattttcat ataaaatcgg agttgaagga
      301 atttatacgg gtttataaag tggaatcggt tgctgtcagg ctgcccgtat atcacaattt
      361 accgaccgat aattatcagc gtaaagtacc ccattgttat cccgatttat tatgtccgta
      421 tgataaaaaa gacaatgtct atttcgtacc tgatcttctg cagtcgctgt ttatagaggt
      481 ggatttaagt aataacacat gtttttacgg agattatgtt attaaatgct attttaccga
      541 taatgacggt aatacggttg cttcaacgaa tgtatcggtt aatataatca attctatgtt
      601 atccgataaa agaattacat ttacacagtg gtttcacggt gactgtttag cttcttatta
      661 cgcagttaaa ccgttaagca aaaaacattt tgcaattatg aagaattttc tcaaaacggc
      721 ttatgagtac ggtattaatg ccatactgac tccggtactt accccgcctt tagataccaa
      781 gataggaggg gagcgaccga caatgcaact ggtggatatt acaaaaacat cttccggtta
      841 tgaatactca tatgatttac tggataaatg gatagatatg tgtaatgaga taggatttga
      901 atatttcgaa atctctcact tttttaccca atggggtgca aagcatgcac cgaaagttgt
      961 agcatatgtc aacggtagga agaaaagaat attcggatgg gagaccgatg caacttccga
     1021 tgaatatatt tcttttctaa gggacttcat atccaatctt ttggtttatc ttaagaataa
     1081 aggtgtagat aaaaggtgtt tctttcatat ttccgacgag cctaatgaga agaatataga
     1141 agggtacaga aatgcaagaa acagcattat cgatctttta gacggttatc ttgtaaccga
     1201 tgccttatct tccatagaat tctacaaaca gggaattgta acatctccca ttcccgcaac
     1261 ggataagatt gaaccctttc ttaaacaaaa catagataat ctatggatat attactgttg
     1321 cggtcagttt attgacgtca gtaacagatt tattgcaatg ccgtcacaga gaaacagaat
     1381 aataggcaca cagttatata aatacagaat ttcaggattt ttacaatggg gctataattt
     1441 ctactataac caatattcca gaaaacttgt taatcccttc cttataaccg acggtgattt
     1501 cttcgctcct gcgggagatg catattcggt atatccggga catgacggta aaccgcttaa
     1561 atccttaaga cttgtagtat ttaatgaagc attacaggat ttgagagcat tcttaaagtt
     1621 ggagtcactg acttcctatg agcatgtgat gaaaattatt gagagtaacg ggaaaatatc
     1681 cttcagtaaa ttccctgaag atatcgaata tattatcaat cttagagaaa gagtaaataa
     1741 ggaaatagat aagctcactg aaaagaaata atataaaagt tatctttatt tactaatacc
     1801 tttttggttt tgtttattca ttaatacagt ttaataaaat atcgtataac aatggtaata
     1861 aaaaaatcct ttattccttt gttaacgcta tattcaataa cggcaataat attgagtaca
     1921 aactttaata ttacggtttt attgatttca gggttgattt ccgttaccgt tctttttatt
     1981 tatactaagg ataaatccgt agcaaagatt attaaggttt tgcttctttc attctttgta
     2041 attgcattta ccgtattttt cttcttttca cgtcttctgt ttatgaatat agaaaatgac
     2101 tattcagatg aaaaccggaa agaagttcga ttgacggggt atgtaacgga aatagttaag
     2161 gatcaagtga ataagtttaa agttatattg aaatgcaata acggttttaa ctattttgtc
     2221 actatgaagg atgattatat aaggataggg gatattattg aactgtcagg actgttgtat
     2281 aagccggaaa gtgcaggtaa taaaggacag tttgattatc aaaaatactg ttattcaaag
     2341 aatatatcgg cggatatata cgtaaacagg ggaaatatat caaagatagg tagtaaaccg
     2401 tttatcgggt ttatagggaa tataagagaa aaagcggtag aaagggtaat gaagcatcta
     2461 aatgaagata aaagaggaat tgttacagca cttatagccg gttcacgtac ttatctggac
     2521 agaaacacaa caagtacatt ccaaaaaagc ggattatcgc acatcttaag catatccggc
     2581 acccacttca acatattaat cattccgttt tattatttat taactttatt atcaaagaac
     2641 aacagattct ctatgatatt cacaatattt gcagtattat ttttacttgt atttacaggc
     2701 ttaagaataa gtgcaatcag agcggcatta tgtattgtta cattattctt ctccaatatg
     2761 ctctatttcg actccaacag gtatctgcct atatgtctct cttcattaat cattattttg
     2821 ataaatcctt tatctgttta cgattacggc tttattatgt cctactgctg tgtggtatcg
     2881 atagcattgt tcagcagaaa aatccgttca ttttttgtat tgcagattga taaattaaag
     2941 aaaaccctgc aggaaaaggt gctgaagaaa catattgtat tggaagacta ttacagaaga
     3001 ataaggaaga caaagttgaa agcagtaatt ctaaaagtac tatccgcact aagtcttcta
     3061 ttatccgttc aacctttaac ggtgtttata tcatacaggt tgtttcactg tatatatcct
     3121 tactctctct tttccaatct tataatcttt atactcataa ctccgttact tttatcgctt
     3181 tggaccgcta tattatttcc ccggataatg tgccccctat tcgggttgtt ggcgaatttt
     3241 ctaattaagt ttgccgaata tatcggcaat ttaccttact caaagctcta tgttaaacat
     3301 atacccgata ttttccttat cattattata tgcacatttt tgtatataaa gtataaacct
     3361 tttaaaagca ggataaataa atatatcgtt atcagtgttg caacgctatt atgcgtaata
     3421 actatttgta taaataatac tccgaaagtc gtttttatgg atgtagggca gggtgattgc
     3481 gccgttataa ccttgtcaaa cggatataac ataatgattg atacaggcga atatatagat
     3541 ttatccgctg ttgcatatcg taca
//
LOCUS       DAOSOM010000082         3532 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_26603_length_3532_cov_4.422203, whole genome shotgun sequence.
ACCESSION   DAOSOM010000082 DAOSOM010000000
VERSION     DAOSOM010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26603_length_3532_cov_4.422203"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="PLF14_09935"
     CDS             complement(<1..787)
                     /locus_tag="PLF14_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71638.1"
                     /translation="MITMKDFPLFIQSPVSYGGHVQGIAVDRKNGHIYFSFTTELLKC
                     DLAGNLLGSVKGWIGHLGCIDFDDETGYVFGSLEIKNDSIGCSIKKLLNTDADIPDSF
                     YIAVFDGKRITSKDIDADKTNVMRTSFIKECTEDYLNKRYGCSGIDGTCVAPDIENND
                     GICDCLYTAYGIYRNNEKDLNDYQIILKYPLKDIIKNSAFFKDNPFHKTGCESFTDKY
                     FVYTGNTNFGVQNLEYDGKTGDWFMAVYRGIKEYFPNYPCFAID"
     gene            complement(784..1839)
                     /locus_tag="PLF14_09940"
     CDS             complement(784..1839)
                     /locus_tag="PLF14_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HOT71639.1"
                     /translation="MKESANRPIFFERNRVFRVYKGGALFNNLFGDVAKDGNYPEEWV
                     ASSVKALNRISKGPKEGISKVLNTDIYLDDLIKECKEEILGPYDDMGILVKVLDSAVR
                     LPVQVHPDKSFSEKYFNSEYGKAEAWIILDTRPGSCIYFGFKEGVTKKDLIAAVEKST
                     EDKHVMEQLIVKHDVKKGEVYFIPARAVHAIGYGCLILEIQEPTDFTIQPEAWCDDYR
                     LNDYEMYMGLDKEIALSCFDMSICGKDAIELSKVRPEVVSKKNNVTVRSLIDNRKTDC
                     FTVNDISIDSGSTILEQGPAIYFVTEGRGSVSYGKNTDPVKKGDYFLLPYCIKGQCTL
                     SSDENITVIECFKGGMS"
     gene            complement(1836..3200)
                     /locus_tag="PLF14_09945"
     CDS             complement(1836..3200)
                     /locus_tag="PLF14_09945"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOT71640.1"
                     /translation="MLALGLDIGTSKICVSVIDGKTGSCPETFDTVNDTFITVDKKQT
                     KMQDAKAILTKAYTLIETVLSKYPDIQCIGVTGQMHGIVYINEDFSLAGPLFTWQYES
                     GDLQYRDSGLSYADYLSELTGYKMATGFGLTTLFYHMHKIVYCKARYISTIHGYIACN
                     LAGCKKPVIHSTDAQSLGLYSLKDNAFDREALKAAGINQNILPDVVNSPIILGKYKNS
                     IPVCVAIGDNQAGFIGSGCDKDILINIGTGSQISLKTDKTSTPCPDIEIRPLSEDKRI
                     FVGASLCGGRALAILKNFYEDVIKMGGAVPPENLYDVMLKNAKISDTESAVEFSTLFC
                     GTRNNPTAKAGITNLTADNFTAADITYATLKGIADELYTMYNSIKKYDNPESNRLIGS
                     GNGIRKNRLLRNILEETFSLPLSVPENTEEAAFGAALYALIATGYFASLDQAQSIVKY
                     EDSI"
     gene            complement(3210..>3532)
                     /locus_tag="PLF14_09950"
     CDS             complement(3210..>3532)
                     /locus_tag="PLF14_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71641.1"
                     /translation="GYASHFGIDPKYFTAPTYEEKGIQFKERVIEPLFFNKDINTVCP
                     YYLYVNVTTAETLRKVLAEPEKYAPSGVYIMRIHGTFVNFLDLSPAIQKDIITRLDPK
                     STSL"
BASE COUNT          921 a          773 c          694 g         1144 t
ORIGIN      
        1 cgtctattgc aaagcagggg tagttgggaa aatactcctt tattcctctg tataccgcca
       61 taaaccagtc accggttttt ccgtcatatt ccagattctg cactccgaaa ttagtattgc
      121 ccgtataaac gaaatattta tccgtaaacg actcgcatcc tgttttatga aaagggttat
      181 ccttgaagaa agcgctgttt ttaatgatat cttttaaagg atattttaat attatctgat
      241 aatcattcag gtctttttcg ttattcctgt atatgccgta agccgtatac agacagtcgc
      301 atataccgtc attgttttct atgtccggag ctacacaagt gccgtcgatt cccgaacatc
      361 cgtatctttt gtttaaataa tcctcggtac attcttttat aaaggaagtc ctcataacat
      421 tggttttatc ggcatcaatg tctttggagg ttattctttt gccgtcaaac acggctatgt
      481 agaaggagtc gggaatatcg gcatcggtgt tcaacagctt ctttatggag caacctatgg
      541 aatcattctt aatctccaat gagccgaata catatcccgt ctcatcatca aaatcaatac
      601 atcccagatg accaatccat ccctttaccg aacctaacag attacctgca agatcgcact
      661 ttaaaagttc ggtggtaaaa gaaaagtata tatgaccgtt ttttctgtcc accgcaatac
      721 cctggacatg ccccccgtaa ctgaccggac tttgaataaa cagcgggaaa tctttcattg
      781 ttatcaactc ataccccctt taaagcattc tattacggtt atattctcat ctgaagataa
      841 tgtacattgt ccttttatac agtaggggag caggaaatag tctccttttt taacagggtc
      901 ggtatttttt ccgtatgata ccgatcctct gccttcggta acaaagtata tggcggggcc
      961 ttgttcaagt attgtactgc ccgaatcaat gcttatgtcg ttgaccgtaa aacagtctgt
     1021 cttacggtta tcgataagtg agcggacggt tacattgttc ttttttgaaa ctacttccgg
     1081 tcgcacctta gataattcta ttgcgtcttt tccgcaaatg gacatatcaa aacagcttaa
     1141 ggcaatctct ttatccaacc ccatatacat ttcgtaatcg ttcagtctgt aatcgtcaca
     1201 ccaggcttca ggctgtatgg taaagtcggt aggttcctga atctccagta tcaggcatcc
     1261 gtaacctatg gcatgtaccg ctcttgcggg tatgaagtaa acctctccct ttttaacatc
     1321 atgctttaca ataagttgct ccattacatg cttatcttcg gtactttttt ccacggcggc
     1381 aataaggtct ttctttgtta ccccttcttt gaatccgaaa tagatacagg aacccggtcg
     1441 ggtatccagt attatccatg cttccgcttt accgtattca ctgttgaaat acttttcgga
     1501 aaaagattta tcgggatgta cctgaacggg aaggcgtact gcactgtcca gtacttttac
     1561 cagtattccc atgtcatcat acggtcccaa tatttcttcc ttgcactctt tgataaggtc
     1621 atcaaggtaa atatcggtat tcaatacttt ggatatgcct tctttggggc ctttacttat
     1681 cctgtttaat gcttttaccg aagaagcaac ccattcttca ggataatttc cgtcttttgc
     1741 aacatcaccg aaaagattat taaaaagagc acctcctttg tatacccgga agactctgtt
     1801 tcgctcaaag aatatggggc ggtttgctga ttctttcata tggaatcctc gtatttcact
     1861 atgctctgtg cctgatccaa agatgcaaaa taccctgttg caataagggc atataaagcg
     1921 gcaccgaaag cggcttcttc ggtattctcg ggaaccgaca gaggcaggga aaatgtttct
     1981 tccagtatgt tccttaataa ccggtttttg cgaataccgt tgcccgaacc tataagtctg
     2041 ttactttccg ggttatcata tttcttgatg gagttataca ttgtgtacag ttcatcggct
     2101 attcccttta aagtagcata ggtaatatcc gctgccgtga aattatccgc agttaggttg
     2161 gttatgcctg ctttagcggt cggattgttt cttgtaccgc aaaacagtgt agaaaattcc
     2221 actgcacttt cagtatcgga tattttcgca tttttcagca taacatcata caggttttcg
     2281 ggcggaacgg ctcctcccat ttttataaca tcctcgtaga agttcttaag tatggctaaa
     2341 gctcttccgc cgcacagcga tgctcctacg aaaatgcgtt tatcttcgga taagggacgt
     2401 atttcaatat cggggcacgg tgttgaagtt ttatccgtct tcaaggatat ctggcttccc
     2461 gttcctatgt ttattagaat atctttgtcg cagcccgacc ctataaatcc cgcctggttg
     2521 tctcctattg caacacagac cggaatactg tttttgtatt tacccaaaat tatcggtgaa
     2581 tttacaacat cgggaagaat gttttgatta ataccggctg ctttcagagc ttctctgtcg
     2641 aaagcattgt ctttcagact gtaaaggccc aaagactgag cgtcggttga atgaataacg
     2701 ggttttttac agcctgcaag attgcatgca atataaccgt ggattgtgga tatatagcgt
     2761 gctttgcagt acactatttt gtgcatatga taaaacaagg tggtaagtcc gaatcccgtg
     2821 gccattttat agcctgtcag ttcggaaaga tagtccgcat aggataaacc gctgtcccgg
     2881 tattgcagat cacccgactc atactgccat gtgaaaagag gaccggctaa ggaaaaatct
     2941 tcatttatat agactattcc gtgcatctgt ccggttacac ctatgcattg tatatcggga
     3001 tattttgaaa gtaccgtttc aataagtgta tatgctttgg ttaatatagc ttttgcatcc
     3061 tgcattttgg tttgtttttt atcaaccgtt atgaaagtat cgttaacggt atcaaaggtt
     3121 tccggacagc tacctgtttt accgtcaatg acggatacgc agattttact tgttcctata
     3181 tccaaaccta atgccagcat aactctctcc tataacgatg tggacttagg atccagtcgg
     3241 gtaataatgt ctttctgaat agcgggggaa aggtccagaa agttaacgaa tgtgccgtga
     3301 atcctcataa tgtaaactcc gctcggagcg tatttttccg gttccgccag tacctttctt
     3361 aatgtttccg ccgttgtgac attcacatag agatagtagg ggcataccgt attgatgtct
     3421 ttattgaaaa acagaggttc aatgactctc tctttaaatt gtatgccttt ttcttcgtat
     3481 gtcggggcgg tgaagtattt gggatcaata ccgaaatgcg atgcataacc gc
//
LOCUS       DAOSOM010000083         3499 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_26937_length_3499_cov_3.838560, whole genome shotgun sequence.
ACCESSION   DAOSOM010000083 DAOSOM010000000
VERSION     DAOSOM010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26937_length_3499_cov_3.838560"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="PLF14_09955"
     CDS             <1..870
                     /locus_tag="PLF14_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOT71642.1"
                     /translation="ALIWGFGFVSQRLGLEAMGVHTFTAPRFILAAFVLGIFLFLRDK
                     KNNVKFTILGNGTEKEKKNLLIAGIACGLIQTVANFFQMSGLKHTTVGKAGFITVLYI
                     FIVPIAGLLLFKKRVTLAVWISVFMALIGSYLLFIQEGFSINKGDLLVLVSAFLFAAH
                     IMVIDHFTKITDSIRMVFLQYAVCGVLSLIAMFVFENPKWDAIMNAWLPIAYAGILSS
                     GVASSLQFVSQKNISAPVASIIMSMESVFAALAGWIFLQESLSLKEIAGCLLIFFGII
                     LAQIPFKQRIRVK"
     gene            936..1298
                     /locus_tag="PLF14_09960"
     CDS             936..1298
                     /locus_tag="PLF14_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOT71643.1"
                     /translation="MNIQESAEMYLETILILSKKSDTVRSIDIVNYTGYTKPSISRAV
                     NLLKKNGYLNIDEKGYITLTASGENIAKNIYERHCVLTDVLLKMGIDDKTAKQDACKI
                     EHVISEETFNKIKEYLNK"
     gene            1313..2242
                     /locus_tag="PLF14_09965"
     CDS             1313..2242
                     /locus_tag="PLF14_09965"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT71644.1"
                     /translation="MTPKQRAVTALNRGIPDEIPTFELEFQLSYELVGKEFLRQEQLE
                     NMSKKEKDIALHENAELMVEVYEKLDYSIIPIHYLWLDEAIKTAKIINRMTNDRYLLI
                     RHGDGTFSIPDGNEMYEFSYRIADDPKGLLEQAEKMAANVIERNKRAIDAGIGSIMLC
                     SDYCYNSGPFLSPYHFSVFITPFLARIIKGIRDAGGYAIKHTDGNIMPILDQLIEANP
                     HALHSLDPMAGVDIAEVKRIAGDKVCLIGNVNCALLQTGTDEEVIESAKYCLTHAKPN
                     GGYIYSTSNVPFKGLPLERYLLILDVWKQYKKY"
     gene            complement(2301..2476)
                     /gene="ssrS"
                     /locus_tag="PLF14_09970"
     ncRNA           complement(2301..2476)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLF14_09970"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(2545..3423)
                     /locus_tag="PLF14_09975"
     CDS             complement(2545..3423)
                     /locus_tag="PLF14_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HOT71645.1"
                     /translation="MKIKYVISDDCIILENLNPDDFNIKDTFECGQCFRWEKTDRQDG
                     YSGIAHGKKLIVQQNEDYITLHCNEKDFLHIWQYYFDLDTDYDEIKERLRKDDIMNKA
                     ICSAPGIRILNQELFETAVSFITSSNSNIKRITRNIKDLCTLLGQPIGDGYYAFPTVE
                     ALSNADICTINQCRAGFRCDYIHSSANMIKNMSDSFNKERYIEMGYEETKKELMRLKG
                     VGNKVADCIILFAGISSSAFPTDVWVKRIMQNLYIKKEVTLDYVSKYGRDKFGDLAGY
                     AQQYLFHYARNNAVHK"
BASE COUNT         1070 a          704 c          692 g         1033 t
ORIGIN      
        1 gccttaatct ggggcttcgg ctttgtatca cagcgattgg gactggaagc aatgggtgta
       61 cataccttta cggcaccccg ttttattctt gccgcatttg tactgggaat atttctgttt
      121 ttaagggata agaagaataa cgtaaagttt actattctcg gaaacggtac ggagaaggaa
      181 aaaaagaatc ttcttattgc cggtatcgct tgcggattaa tacagacagt tgctaacttc
      241 tttcagatgt cgggattaaa acatacaacc gtgggtaaag cgggctttat aacggtactg
      301 tatatattta ttgttccgat tgccggttta ttattgttca aaaagagggt taccttagcg
      361 gtatggataa gcgtatttat ggctttgata ggttcttatc tgctttttat tcaggaagga
      421 ttcagtataa ataaagggga tcttctggta ctggtaagtg cattcttatt tgccgcacac
      481 ataatggtaa tagaccattt tacaaagata accgacagca tcagaatggt attcctgcaa
      541 tatgcggtat gcggtgtgct gtcacttata gccatgttcg tgtttgaaaa tcctaaatgg
      601 gatgcaataa tgaatgcatg gcttcccata gcatatgccg gcatattgtc atcgggtgtt
      661 gcctccagcc tgcagtttgt aagccagaag aatatttccg ctcccgtagc ttccataata
      721 atgagcatgg aatcggtatt tgccgctctt gcaggttgga tatttttaca ggagagtctt
      781 tcattaaaag aaatagcggg atgtctgttg attttcttcg gaataatact tgctcagatt
      841 cccttcaaac agcgaatacg ggtaaaatag gcctcactgt ttttttatat ttctttatga
      901 tacaatttaa taaaatcctt aggaaaggct gagtcatgaa catacaggaa tccgctgaaa
      961 tgtatctgga aactatactg attctgtcga aaaagtccga cacggtacgt tccatagata
     1021 tagtaaacta cacggggtat acaaaaccca gcataagccg ggctgtcaac ttgttgaaaa
     1081 aaaacggcta tctgaatata gatgaaaagg gatatatcac tttaaccgct tcgggagaaa
     1141 atatagcaaa aaacatctat gaacggcact gtgtgctgac cgatgttctt cttaaaatgg
     1201 gtatagatga taagacggca aaacaggatg cttgcaaaat agagcatgtt ataagtgagg
     1261 aaacctttaa taaaattaaa gagtatttaa ataagtagca tcgggaggaa ttatgacacc
     1321 taaacaaaga gccgtaaccg cgcttaacag gggtattccc gatgaaatac ctacatttga
     1381 actggaattc cagctttcct atgaattagt aggtaaagag tttcttcggc aggaacagct
     1441 ggagaatatg agcaagaaag aaaaagacat tgcactgcat gaaaatgccg agctgatggt
     1501 ggaagtatac gaaaagctcg actattccat aataccgatt cactatcttt ggctggatga
     1561 ggccataaag accgcaaaga ttataaacag aatgacaaac gacagatacc ttctcataag
     1621 gcacggagac ggaacattca gcatcccgga cggcaatgaa atgtatgaat tttcttacag
     1681 aattgccgat gaccccaaag gtcttctgga gcaggcggaa aagatggctg caaatgtgat
     1741 agagaggaat aaaagggcca ttgatgcggg aataggcagt ataatgctct gttccgatta
     1801 ctgctataac agcggacctt tcttatcacc ttaccatttc tccgtattta taaccccctt
     1861 tttggcaagg ataataaaag gtataagaga tgcgggcgga tatgcaatca aacataccga
     1921 cggcaacata atgcccatac tggaccagct tatagaagcc aatccccatg cccttcactc
     1981 gcttgacccc atggcgggag tggatattgc cgaagttaaa agaattgccg gagacaaagt
     2041 gtgtcttata ggtaatgtca actgtgccct gcttcagacc ggaaccgatg aagaagttat
     2101 agaaagtgca aaatactgtc tcacccacgc caaacctaac ggtggttaca tatattccac
     2161 cagtaatgta cctttcaaag gactgccgct tgaaagatac ctgcttatac tggatgtctg
     2221 gaaacaatat aaaaaatatt aaatgagaca aaaaccttta ttgcttctat tattaaattt
     2281 atataaaaaa gagaaatata aaaattccca aaataccggc cctttttttt gacgcctagc
     2341 gtccgccagg tgggttccgt gatgagtgtt tctgccttcc gctgatatac ggcctgacat
     2401 ccgcacacat acaactaaat cccgtcaaat tactgtaggt tcaaatacaa agggttatcg
     2461 tgtatctcag gaatctacta cccaaattat acaatattac cggtttttca tcaatattta
     2521 atattcctct aagatatgat ataattattt atgaacggca ttatttcgtg catagtggaa
     2581 aagatactgc tgtgcatagc ctgccaaatc accgaatttg tcccgtccgt atttcgaaac
     2641 gtagtccaga gtgacttctt tttttatgta taagttctgc attatccttt taacccacac
     2701 atcggtggga aaagcggaag aagaaatgcc cgcaaataaa ataatacaat cggcaacctt
     2761 attccctact cctttaagtc tcataagctc cttctttgtc tcttcataac ccatttctat
     2821 atatctttcc ttattgaaag aatcggacat attcttaatc atgtttgcag atgagtgtat
     2881 atagtcacaa cggaatcccg ctctgcattg atttattgtg catatatccg cattacttaa
     2941 agcttccacg gtcggaaaag cataataacc gtcgcctatc ggctgcccca acagtgtgca
     3001 caaatctttt atattcctcg ttatcctttt aatattactg ttagatgatg ttataaaact
     3061 cacagcggtc tcaaacagct cctgattgag tatccttata ccgggagcgg agcatatggc
     3121 cttgttcatg atatcatctt ttctaagtcg ttcctttatc tcatcgtaat cggtatccaa
     3181 atcaaagtaa tactgccaga tatgtaaaaa gtccttttca ttgcaatgta atgttatata
     3241 atcctcattt tgctgcacga taagcttctt gccgtgagct atacctgaat aaccgtcttg
     3301 tctgtctgtt ttttcccatc ggaaacactg accgcactca aacgtatcct taatgttaaa
     3361 gtcgtcggga tttaagtttt ccagtatgat acagtcatca cttatgacat atttaatctt
     3421 catattctat gcacagcatg caatggcctt ccagttcatc cagtgtaaat cgcgctttac
     3481 cgcctttccc cgtaaactt
//
LOCUS       DAOSOM010000084         3066 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_31927_length_3066_cov_4.326802, whole genome shotgun sequence.
ACCESSION   DAOSOM010000084 DAOSOM010000000
VERSION     DAOSOM010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31927_length_3066_cov_4.326802"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1247)
                     /locus_tag="PLF14_09980"
     CDS             complement(<1..1247)
                     /locus_tag="PLF14_09980"
                     /inference="COORDINATES: protein motif:HMM:NF016144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HOT71646.1"
                     /translation="MKSIIGLKRGTVKLHKHKKQWRTIAAETIAMLYEILGDTACDIQ
                     HVGSTSVVHIKAKPVIDIAVAVNSFSDFDGYIPALEARGVKYRPKVNIGNERFFVIGD
                     ESDFFTHHIHVVPVTSREWINYINFRNYLNAKPFAAGQYEEVKINLLKKYKHNRKAYT
                     DGKAEIIAKLLKEAFAWSYLGKTVTVTVLKSLSEKCVPVYSGYIEGVTGDNESQEVYV
                     IGVNNPGSVYTGTVTAIIYGKDNTPGKWVVAPAEASFNQAQIAEVILPFEQDTDVFID
                     SVHRKSCGVVVYRIVDGNIEYLLVKEYYCYGWSIPKGHMEAGESEADTAIREAWEEVG
                     VRVTPDMEFIRTVEYTIQPVYKKEVVFRISEFKGESRVVKPGIEETGWFVLSEAKKLL
                     KYQETCAVMEDAEVYIAGLHKGG"
     gene            complement(1412..2419)
                     /locus_tag="PLF14_09985"
     CDS             complement(1412..2419)
                     /locus_tag="PLF14_09985"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71647.1"
                     /translation="MYNELRMKKSRNPIYLHLELDTKYCSNEDLHTLKKYACIKYGST
                     ISRDVLVPHDITIRALHYTIMKLFGWMGRHDYTMKLPSQIYDNVTETDSNIIISLMGV
                     LLKCPDEVKIDDDFLVSFDDTGPKDFENRFSEYYTGPYANFASIEDYSTLSGILTLCD
                     SIKDEIMGVDCNKLLGRLKVSSILTYEGDNISGNVEYLKGDSIKEYNMRREHCKECEM
                     QPDVLPLTYKLVYLYDSERKWTVNITMPKDFSVLINDRGVSKEDIDFAKNKVLDSYKP
                     YCIFRDGANVMEDVANLPGFCDFLRVVFERSDTNKKKTALKYARKRDWYPDEIGDCIM
                     F"
     gene            complement(2457..>3066)
                     /locus_tag="PLF14_09990"
     CDS             complement(2457..>3066)
                     /locus_tag="PLF14_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71648.1"
                     /translation="HSVIKTAVQMELEKLSDNNDSVAAFNIENLSTFTSGDVKKDDFL
                     KENANTLLPCMYVSSVLAAKGEKVGTDQEIPKHGRYYRNIEDTEVLPLTHKLIYTYGG
                     ESKWVVNIVRLKNFSSLIEDGLVCMSRVNDAENAVKEKHRPFCVFKDGANVLDDVDDL
                     PGYCEFLHDIFEGCNIEKMKKALKFGRRRDWHIEEMNEPIIL"
BASE COUNT          864 a          718 c          425 g         1059 t
ORIGIN      
        1 ttccctccct tatgcagacc tgctatatat acttcggcat cttccattac ggcacatgtc
       61 tcttgatact ttaaaagttt ctttgcttcg gagagtacaa accatccggt ttcttctatc
      121 cccggtttta caaccctgct ctctccttta aattccgata tacggaaaac aacctctttt
      181 ttatacaccg gctgtattgt atactccact gtccttataa actccatatc gggtgttact
      241 cttacaccta cttcttccca ggcttctctt atcgccgtat cggcttcact ctcacccgct
      301 tccatatgtc ctttgggaat actccatccg taacagtaat attcttttac cagaaggtat
      361 tctatattcc cgtccactat tctgtatact accacaccgc acgacttacg gtgtacactg
      421 tcgataaata catcggtatc ctgctcaaag ggtaatatca cttccgcaat ctgtgcctga
      481 ttaaatgaag cttcagcagg tgctaccacc catttacccg gagtgttgtc cttcccatat
      541 attatagcgg taacggtacc cgtataaacg gaacctggat tatttacccc tataacatat
      601 acttcctgag actcattatc acccgtaacc ccctctatat acccgctgta aacgggaaca
      661 catttctccg acagagattt taaaacggtt acggttactg tctttcccag atatgaccat
      721 gcaaaagctt ctttcaataa ttttgctatt atctccgctt taccgtccgt atacgccttt
      781 ctgttgtgct tatacttctt cagaagatta atcttcactt cttcatactg cccggctgca
      841 aagggtttgg catttaagta gtttctgaag tttatatagt ttatccattc acgacttgta
      901 acgggaacca catgtatatg atgtgtaaaa aaatcacttt catcaccgat aacaaaaaac
      961 ctctcgttgc ctatattaac cttcggtctg tatttgacac ccctggcttc cagggcggga
     1021 atatacccat caaaatcgga aaagctgttt accgctaccg ctatatctat aacgggtttt
     1081 gcctttatat gcacaacgga agtgcttccc acatgctgta tatcgcaagc ggtatccccc
     1141 agtatttcat ataacatggc tattgtctcg gcagcaatcg ttctccattg ctttttatgt
     1201 ttatgaagtt ttaccgttcc ccttttcagc ccgattatgc ttttcattat gcggcatatt
     1261 ctaccacagg tttatccgca tgtaaacgga ccaatcatat taatgcacaa attttgcacc
     1321 atcttttatg cgatttataa catattgaat aatatgtaat atagttaata aatatttgct
     1381 taattaataa acttttatga gatactctat gctaaaacat tatacagtca ccgatttcat
     1441 ccggatacca atctctttta cgggcatatt taagtgcagt tttcttttta ttcgtatcgc
     1501 ttctctcaaa aacaactctt aagaaatcac agaatccggg caggttggct acatcctcca
     1561 taacatttgc tccgtcacgg aaaatacagt aaggcttata actgtccaaa actttattct
     1621 ttgcaaaatc tatatcttct tttgaaacac ctctatcatt aatcaatact gaaaagtcct
     1681 taggcattgt aatattaact gtccattttc tttcgctgtc atacaaataa accagcttat
     1741 atgtcaaagg caaaacatcc ggttgcattt cgcactcttt gcaatgttcc cttctcatat
     1801 tgtattcttt tattgagtca ccttttaaat attcaacatt cccgcttata ttatcacctt
     1861 catatgtcaa tatggaggac accttaagtc ttcccaacaa tttgttacaa tcaactccca
     1921 ttatttcatc ttttattgaa tcacacaatg taagtatacc cgataatgtg ctgtaatcct
     1981 ctatacttgc aaaatttgca tatggtcccg tgtaatactc ggaaaacctg ttctcaaagt
     2041 cttttggacc agtatcatca aatgatacaa ggaaatcatc atcgattttc acctcatcgg
     2101 gacatttgag taaaaccccc atcaaactga ttattatatt cgaatcagtt tcggtcacat
     2161 tatcatatat ttgactggga agcttcatag tataatcatg tcttcccatc catccgaaga
     2221 gtttcataat tgtataatgt aatgctctga tagttatatc gtgaggcact aaaacatctc
     2281 tggatatagt agaaccatat tttatgcagg catatttttt cagtgtatgt aagtcttcat
     2341 tggaacaata ttttgtatcc agttccagat gaagatatat aggattacgg ctttttttca
     2401 ttctcaattc gttatacata acaagactcc ttatatattt attttatcca cttaaattac
     2461 aatattatcg gttcattcat ttcttcaata tgccaatctc ttctgcgccc gaattttaaa
     2521 gcttttttca tcttttcaat attgcaacct tcaaatatat catggagaaa ctcgcagtaa
     2581 ccgggtaaat catctacatc atccagtaca tttgcaccgt ctttaaaaac acaaaacggt
     2641 ctatgttttt cttttacagc attttccgca tcatttaccc gactcataca taccaaaccg
     2701 tcttcaataa gtgagctgaa attcttcaac cttacgatat taacaaccca cttactttca
     2761 ccgccatagg tatatatgag tttatgtgtc aaaggtaaga cctcagtatc ttcaatattt
     2821 ctataatatc taccgtgctt cggaatctcc tgatcggttc ctaccttttc accttttgca
     2881 gccaataccg aagacacata catgcatggc aacagtgtat ttgcattctc tttcaaaaaa
     2941 tcatcttttt ttacatcacc ggatgtaaag gtacttaaat tttcaatgtt aaatgcagca
     3001 actgaatcat tattatctga aagtttttcc aattccatct gaaccgccgt ttttattaca
     3061 ctgtgc
//
LOCUS       DAOSOM010000085         3062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_31990_length_3062_cov_7.012637, whole genome shotgun sequence.
ACCESSION   DAOSOM010000085 DAOSOM010000000
VERSION     DAOSOM010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31990_length_3062_cov_7.012637"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3062
                     /locus_tag="PLF14_09995"
     CDS             <1..>3062
                     /locus_tag="PLF14_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOT71649.1"
                     /translation="VRLGEFFDTTDAFGYYQIDAREAGTYEISASLRGYTTVTDNNFE
                     LNGTKDWFAELTLDSDPCIDIYASVKCVYSGIPLKGVEVRARHSSEGLFYDNTDSDGS
                     VVFYNLPKGKYTFEVNVNGKPGWESYISGEYDVTQDHLLNCSLKPKYKSLTVETYGFC
                     AFTEQDDSPLRNVYVRLHGLDPNDHDVELISPAPIKTGEDGTVVFDKLVPIDWLIEVY
                     SPGYDPMEKLVKAKADGTFEQDTVRMDMEFHPGTLIAKLDSLYDDDERYCGLEVHLCG
                     NDAITEGLTRTITVESWHRPKRAAIFENLLPGQYKIYTYGSCENIVRINAGDLQVYFN
                     RLVDSSISPPRRLSITFQGETQAVAKMGRETEVELPLDVEKANYSGFLHKIDMDEEGA
                     LITSPAANTVMTLHPSEYFPQNPEYGKTYTVTTDEFGFYSFNVYPGLYGVTVSDMDDY
                     WGAFATYRQRYGMVEQSEEGGELGKPGKMGGWPYYQKWQGSHDSAIAYMGYGKLDGGK
                     KYTNNTGYADIGGVPLSSGITEGDLFMKEKVCSISVETLPARIEKGWYAVTGKINDGN
                     PDNHEAANYEFSKTWFDLANFRTELSCGEETIDNDTSPFPHKFTQLQPGTHDVNFTKK
                     AGATMPEYSLHRKGVSVQEYHQNWEGTDVTFYDFPAPGILPAEFPSDYCSTYDTVWPC
                     GEMDIDIDVRAEELTRWTAPRDHLEEHVYYFVELDKNTGEGEYRCYKDSDYYELNRHF
                     YGWTSDIIPNKLFSAEDVHWYGTISYPNYVPITLYFHLLAGYEYKWDGSREFWEFIPS
                     RWFEMTYDPSTGEGGPTIYIGGPKNNVREGLGNAPNILSPFKAIIKAKNYYTGEEITG
                     ISFVNDTAGQVFFSENEYDNQIYLKETFSNDDINVGDKNLMFHQASFEPDFRGDNRTV
                     TLTLYMVPNIGLDLTVKDNNDNPVRGATVQLRSAKGLDELLRTDDLNGVVNVKNLRDE
                     CYTLIIDAAGCNTYTYELTADMLQPDGYGWMVFIHEAILTPITRPEVS"
BASE COUNT          943 a          568 c          722 g          829 t
ORIGIN      
        1 ggtaagattg ggagaattct ttgatactac cgatgctttc gggtattatc agatagatgc
       61 ccgtgaggcg ggaacgtatg aaatatccgc atcacttaga ggctatacta cggttacgga
      121 taacaatttt gagttaaacg gcacaaaaga ctggtttgcc gaactgacac ttgattcaga
      181 cccctgcata gatatatatg catcggtcaa gtgtgtttat tccggtattc cgctaaaagg
      241 agtagaggtt cgggcaagac acagtagcga gggactattt tacgataata ccgacagtga
      301 cggaagtgtc gtattctaca atttacctaa gggaaaatat acttttgagg taaacgtaaa
      361 cggtaagccg ggatgggaaa gctatatatc aggtgaatat gacgtaacac aggaccactt
      421 actaaactgt tctttaaagc ctaaatataa aagccttacg gtggaaacct acggcttttg
      481 tgcttttacc gaacaggatg acagtccttt acgtaatgtc tatgtaagac ttcacggcct
      541 ggatcctaat gatcacgatg tggagctcat atctcccgca ccgataaaaa cgggagaaga
      601 cggaacggtg gtatttgata aacttgtacc tattgattgg ttaatagaag tatattcacc
      661 gggatatgat cccatggaaa agttagtaaa agccaaagct gacggaacat ttgaacagga
      721 cacggttcga atggatatgg aatttcatcc cggaacattg attgcaaagc ttgattcgct
      781 atatgatgat gacgaaagat attgcggtct ggaagtacat ctttgcggta atgatgccat
      841 aacggaaggt ttgacacgta ccatcactgt cgaatcatgg cataggccta aaagggctgc
      901 tatttttgaa aaccttttac cggggcaata caagatttat acttacggaa gttgcgagaa
      961 cattgtgcga ataaatgcgg gagacctaca ggtttatttt aatcgacttg tggattcgtc
     1021 gatttcgcct ccaagaaggc tttccataac tttccagggt gagacacagg ctgttgccaa
     1081 gatgggtcga gaaacagagg tggaattacc tttggatgtt gaaaaggcaa attattcggg
     1141 tttcctgcat aaaatagata tggatgagga aggtgctctt ataacttcgc ctgccgccaa
     1201 taccgtaatg acattgcatc cttcggaata ttttcctcag aatcccgaat acggtaaaac
     1261 ctataccgtg acaaccgatg agttcggttt ctattctttc aatgtttatc ccggattata
     1321 tggtgtgacc gtttccgata tggacgatta ctggggagct tttgcaacct atcgtcagcg
     1381 ctacggaatg gttgaacaaa gtgaagaagg cggagaactg ggtaaacctg gaaaaatggg
     1441 cggatggcca tattatcaga aatggcaagg cagccacgat tcggccatag cttacatggg
     1501 gtatggtaaa ttagacggtg gtaaaaaata cacaaacaat acaggatatg ctgacatagg
     1561 cggggtgccg ctctcctccg gaattaccga gggagacctg tttatgaaag aaaaagtctg
     1621 ctctataagt gtggagactt tacctgctag aatagaaaaa gggtggtatg ctgttaccgg
     1681 taagataaat gatgggaatc ctgacaatca tgaagctgcc aattatgagt tttcaaaaac
     1741 atggttcgat cttgccaact tcagaacgga gctgtcctgc ggcgaggaaa caattgataa
     1801 cgatacatct ccttttcctc acaaatttac tcaactccaa ccgggaactc atgatgtcaa
     1861 tttcactaaa aaagccggtg caacaatgcc ggagtacagc ttgcaccgca aaggagtatc
     1921 cgtacaggaa tatcatcaaa actgggaagg aaccgatgtc acattttatg attttcctgc
     1981 acccggtata cttcctgcag aatttccctc tgattactgc agcacatacg atactgtctg
     2041 gccttgcggg gaaatggata ttgatattga tgtacgcgct gaagagctga ctcgttggac
     2101 ggctccgaga gaccaccttg aagaacatgt ttattatttt gtagaactgg ataagaatac
     2161 aggtgagggc gaatatagat gctataaaga ctctgactac tacgaattaa ataggcattt
     2221 ttacggatgg acttcggata taattcccaa taaactgttt tccgcagagg atgtgcattg
     2281 gtatggtact atttcctatc cgaattatgt accgatcacc ctatatttcc acttacttgc
     2341 cggctacgaa tataagtggg atggtagtag ggagttttgg gaatttatac ccagtagatg
     2401 gtttgagatg acatatgatc cgagtacagg agagggaggt cctacaatat atattggcgg
     2461 tcctaagaat aatgtacgtg agggtttagg taatgctccg aacattttaa gtcctttcaa
     2521 ggcaataata aaggctaaaa actactatac aggagaagaa ataacgggta tatcttttgt
     2581 taatgacaca gccggacagg tctttttttc cgaaaacgaa tatgataatc aaatctattt
     2641 aaaggaaaca ttttcaaatg atgacataaa tgtgggtgac aaaaacctta tgttccatca
     2701 agcctccttt gagccagatt ttagaggaga caatcgtaca gtcaccctta ctttatacat
     2761 ggtgccgaat atagggctgg atttaactgt aaaagacaat aacgataatc cggtgcgggg
     2821 tgctacggtt cagttgagga gcgctaaagg tttggatgag cttctgagaa cagatgattt
     2881 aaatggcgtg gtcaacgtaa aaaatctcag agacgaatgt tataccttaa ttatagatgc
     2941 tgcgggttgt aacacatata cttatgagtt gacagccgat atgttacaac ctgacgggta
     3001 tggctggatg gtctttatac acgaagcaat attaacaccg ataacaagac cggaagtttc
     3061 ca
//
LOCUS       DAOSOM010000086         2972 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_33257_length_2972_cov_5.761056, whole genome shotgun sequence.
ACCESSION   DAOSOM010000086 DAOSOM010000000
VERSION     DAOSOM010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33257_length_2972_cov_5.761056"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2972
                     /locus_tag="PLF14_10000"
     CDS             <1..>2972
                     /locus_tag="PLF14_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOT71650.1"
                     /translation="VRLGEFFDTTDAFGYYQIYAHEAGNYEISASLRGYTTVTENFEL
                     NGTKDWFAELTLDEDPCIDIYASVKCVYSGIALEGVEVRARHSSEGLFYEYTDSDGSV
                     VFYNLPRGKYTFEVNVNGQPGWESYISGEYSVTQDQLLNCSLKPNYQSLTVETYGFCA
                     FTEQADSPLRNVYVRLHGLDPNDHDVELISPAPIKTGEDGTVVFDELVPIDWLIEVYS
                     PGYDPEEAIVKAMPDGTFDQDTVRMNMVFHPGTLTANLDSIYDDNEIYSGLEVHLCGN
                     NDTVTEGLTRTVTLGAGETAATFGNLLPGQYKIYTYGSCERNVPIPIQGQNIYKHQGL
                     SAWNSPERVFSVTFRGETQAVAKMDQQIDTDLPLDVEKANYSGFLKKIDMDDEGALKT
                     SPAANTVMTLVPSEYYPQNPEYGKTNTVTTDEFGFYSFNVYPGLYGVTVSDMDDYWGA
                     FANYRQCYGMVEQSGGTSISVKGGGWPYYQKWQGSYESALAYMGYGPYGRYKTGYADL
                     GGMPLSSGITEGDLFMKEKVCSVYVDTSYFGQSAWWQTVSGEIDDDNYYPGYKFSRTW
                     FDPANFRTETSCGEITVDNDISPLPHRFLNLQPGTHNVNITKKAGVTRTPQYILKRRE
                     LDRDGVLQIYSWDGTNVMFYDFPSPGILPADFPSDYCNTPDTVWPCGENYLNIIVNGT
                     DMQWNWSGFTAHIYREVEEEGNRYYKSLAEDGGYLGFNIYAWSSDIIPNRLFNTVMQY
                     SWSEDEDHKIVLPNIPLTLYFHSYYKDPCEWFEMEYNPSTGAGSPKIYLDGPKKNTRL
                     GLGAAPGILKPFTAKIRAKNYYTGEEITGISFVNKDTGKVYVSGTDYPNEIFVKDTYS
                     RDEINAGGKNLIYSYAVLERDYEGANYTVTLTLYMKPNIDLDLTVKDNNGNPIPSATV
                     QLRSPKGFDALFKTGADGLARGWSLKDENFTLVVDAPGYNTYTCELTADMLQPDGHGY
                     MVFIHEAILTPITRPEVS"
BASE COUNT          903 a          575 c          696 g          798 t
ORIGIN      
        1 ggtaagattg ggagaattct ttgatactac cgatgctttc gggtattatc agatatatgc
       61 ccatgaagcg ggaaattatg aaatatccgc ttcgcttaga ggttatacta cggtaacgga
      121 aaattttgag ttaaacggca caaaagactg gtttgccgaa ctgacacttg atgaagaccc
      181 ttgcatagat atatatgcat cggtcaagtg tgtttattca ggcattgcgc ttgagggagt
      241 agaggttcgg gcaagacaca gcagcgaggg actattttac gagtataccg acagtgacgg
      301 aagtgtcgta ttctacaatt tacccagagg aaaatatact tttgaggtaa acgtaaacgg
      361 tcagccggga tgggaaagct atatatcagg tgaatattcc gtaacacagg accagttgct
      421 aaactgttct ttaaagccaa attatcaaag ccttacggtg gaaacctacg gcttttgtgc
      481 ttttaccgaa caggctgaca gccccttacg taatgtctat gtaagacttc atggcttgga
      541 tcctaatgat cacgatgtgg agctcatctc tcccgcaccg ataaagacgg gagaagacgg
      601 aacggtggta tttgatgagc ttgtacctat tgattggtta atagaagtat attcaccggg
      661 atatgatccc gaagaagcaa tagtaaaagc tatgcctgac ggaacatttg accaggacac
      721 ggttcgaatg aatatggtat ttcatcccgg aaccctgact gcaaatcttg attcgatata
      781 tgatgacaat gagatatatt ccggtctgga agtacatctt tgcggtaaca atgataccgt
      841 aacggaaggg ctgacacgta ccgtcactct cggagcaggg gaaactgctg ctactttcgg
      901 aaacctttta ccgggacaat acaaaattta cacttacgga agttgcgagc gcaatgtacc
      961 cataccgata caaggccaaa atatctataa acatcaaggt ttaagtgcat ggaattcgcc
     1021 tgaaagggta ttttccgtaa ctttccgggg tgagacacag gctgttgcaa aaatggatca
     1081 gcaaatagat acggatttac ctttggatgt tgaaaaggca aattattcgg gttttctaaa
     1141 aaaaatagat atggatgatg aaggtgctct taaaacttcg cctgccgcca ataccgtaat
     1201 gacattggtt ccttcggaat attatcctca gaatcccgaa tacggcaaga ccaacaccgt
     1261 gacaaccgat gagttcggtt tctattcttt caatgtttac cccggattat atggtgtgac
     1321 tgtttccgat atggacgatt actggggagc ttttgcaaac tatcgtcagt gttacggaat
     1381 ggttgaacaa agcggaggaa cctctatctc tgtaaaagga ggcggatggc cgtattatca
     1441 gaaatggcaa ggcagctacg agtcggcctt agcttacatg ggatacggtc cgtatggtag
     1501 atacaaaacg ggatatgcgg acttgggagg tatgccgctc tcctccggta ttaccgaggg
     1561 agacctgttt atgaaagaaa aagtctgttc cgtgtatgtg gatacgtcct atttcggaca
     1621 gagcgcctgg tggcagactg tttccggcga gatagatgat gataactatt atcccgggta
     1681 taagttttca agaacatggt tcgaccctgc caacttcaga accgagacat cctgcggcga
     1741 gattacagtt gataacgata tatctcctct tccgcacaga tttcttaatc tccaaccggg
     1801 cactcataat gtcaatatca ctaaaaaagc cggtgtaacg agaacaccgc aatatatatt
     1861 aaagcgcaga gaattggacc gggacggtgt attacagatt tatagctggg acggtacaaa
     1921 tgtcatgttt tatgattttc cttcacccgg catacttcct gcagattttc cttctgatta
     1981 ctgcaacacg cccgatactg tctggccttg cggggaaaat tatttgaata ttatagtaaa
     2041 cggtacagac atgcaatgga actggagtgg ctttactgca catatttatc gtgaagtaga
     2101 agaagaaggt aatcgctatt ataaaagtct ggcagaagac ggggggtatt tgggttttaa
     2161 tatatacgca tggagttcgg atataattcc taatcgactt tttaataccg tgatgcagta
     2221 tagttggagc gaggatgagg atcataaaat tgtccttccg aatataccgc tcaccctata
     2281 tttccacagt tactacaagg acccctgtga atggtttgag atggaatata atccgagtac
     2341 aggagcggga agtcctaaaa tatatcttga cggtcctaag aaaaatacac gtttgggctt
     2401 aggtgcggct ccgggaatat taaaaccttt cacggcaaaa ataagggcta aaaactacta
     2461 tacaggagaa gaaataaccg gcatatcttt tgttaataaa gatacgggaa aggtctatgt
     2521 ttcgggaacc gactatccta atgaaatatt tgtaaaggat acatattcaa gggatgaaat
     2581 aaatgcgggc ggcaaaaacc ttatatattc ttatgccgtc ttagagcgag attatgaagg
     2641 agccaactat acagtcaccc ttaccttata catgaaaccg aatatagatt tggatttaac
     2701 tgttaaagac aataacggta atcctatccc gagtgctacg gttcagttga ggagtccaaa
     2761 aggttttgat gcacttttta aaacaggtgc agatggcttg gccagaggat ggagcctcaa
     2821 agacgaaaac tttaccttag ttgtagatgc tccgggttac aacacatata cttgtgaact
     2881 gacagccgat atgttacagc ctgacgggca tggctatatg gtctttatac acgaagcaat
     2941 attaacaccg ataacaagac cggaagtttc ca
//
LOCUS       DAOSOM010000087         2892 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_34456_length_2892_cov_5.225238, whole genome shotgun sequence.
ACCESSION   DAOSOM010000087 DAOSOM010000000
VERSION     DAOSOM010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34456_length_2892_cov_5.225238"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2892)
                     /locus_tag="PLF14_10005"
     CDS             complement(<1..>2892)
                     /locus_tag="PLF14_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT71651.1"
                     /translation="TDLSYLSYAKIDNSLEEFLSSDENSTWNTIIANKKTAKESVKIV
                     TVNNIESVPLFASGRAYITQGVSFTEQDYSSGNKVCLISDQLAKSNNIKVGDVLKLSL
                     WNAGYELKTSFLETAWTPKSHGNKNALSDSENYTVAGIYLDDDAWNTQCCTFTPNTVF
                     VPELSINDSMKLQSSLTDLGGDRFSGIPDSRTYILKTGKASDFEAEMKTAGYGGIFYY
                     YDKNDPSLPYLYALSDNFRITTYISTAVLAVFIISMAMHAIVINLASTDKKVRLRAVL
                     TVIIISIISIAISAVMSLSFYGSVINTTNEYLTTKASTGKALHTDSSLPLPSASIEQE
                     KLDSVPTPLLMIFAVQTGILLLILCISSVLCFAIKGGKKKTKIPYRLKKGEGIKGGMP
                     AVIWYSITQSLRSNVMGMLIVVLVTLSGIFMFLSVSTWLTAKNSISEAEKAYTTIAVA
                     SENEFLKNVNTYTKGIYEYNSLADIYEAAEQSEHVEQVDIRDKCMAYNNGIKTISSGI
                     VDEFTPITAVDYPYDLSVVVGTCVSVEYTVGAERENVFGSFGDTDVFLAYTAVLELDR
                     KESPVLSEEYDYSKYIRVDTRQFGTDPNEYFRVGERYILYGSKGYSQYPKTERYRDNP
                     APFDLETILPEGKYTLINMNREDPDFAFIHFSPQMHEILGTDKDTKVERKSSGKKVSY
                     NINYCYFCAYRDDTNVAYSRLDGSLEEFLASDKGEKWKRHIEECKTTINSLKMNFTKN
                     LDSIPLFNQGLAYITEGRTFTEQEYSEGAKVCIISAETARESGIKIGDKIDFKFWSNG
                     YIVTSNSKQHLWQHASFSKENGFIDEGTFEVIGIYKAENVWSEKEFFFTPNSAFAPKG
                     SVKKPIDLDLPTFIINGKYIEETGEYIPATTGKYQSIPNTRSFVIRTGHIEAFEKEME
                     SLGYGGMFYYYDQGFSMISPILNSLRESTSLLMYISVAVWIVVMSAFFI"
BASE COUNT          841 a          588 c          532 g          931 t
ORIGIN      
        1 tgataaagaa tgccgacatt accactatcc atacagcaac ggatatatac ataaggagtg
       61 aggtgctttc cctaagagaa ttgagaatag gagatatcat ggaaaacccc tggtcatagt
      121 agtagaacat tcctccgtat cccagcgatt ccatctcttt ttcaaaagcc tcaatatgac
      181 cggtcctaat gacaaacgag cgggtgttgg ggatactttg atacttgccc gtagtagcag
      241 gaatatattc acctgtttct tcaatatatt taccgttaat tatgaaagtg ggaagatcaa
      301 ggtctatagg ctttttaaca gaccctttag gtgcgaaagc actattggga gtaaagaaga
      361 attccttttc gctccaaaca ttctctgctt tgtatatacc tatgacctcg aaggtgcctt
      421 catcaatgaa accgttttct ttactgaatg aagcatgctg ccacaaatgt tgcttggaat
      481 ttgaagttac gatatatccg ttagaccaaa atttaaagtc tattttgtct cctatcttta
      541 ttccgctttc tctcgcggtt tccgcactga tgatgcatac ttttgctcct tcggaatact
      601 cctgttccgt gaaagttcga ccttcggtaa tgtatgcaag accttgattg aacagaggta
      661 tactatcaag atttttcgtg aaattcattt taagtgaatt gatggttgtt ttacattcct
      721 ctatgtgcct tttccatttt tctcctttat cggaagcgag aaactcttcg agtgaaccgt
      781 caagtctgct ataagcaaca tttgtatcgt ctctgtatgc acagaaataa caataattta
      841 tattatagga gacttttttc ccggaacttt tacgctccac tttagtgtct ttgtcagtcc
      901 ccagaatctc gtgcatttgg gggctaaaat gaataaacgc aaagtcggga tcttctctgt
      961 tcatattaat aagtgtatac ttgccttccg gcagtatcgt ttcaagatca aacggggcag
     1021 gattatccct atatctttcc gtcttgggat attgcgaata accctttgat ccgtataaaa
     1081 tgtatctttc tccaactcga aagtattcgt tcggatcggt accaaactgc cgagtatcaa
     1141 cccttatata cttgctgtaa tcatattctt cacttagcac gggggattcc ttacgatcca
     1201 gttccagtac tgccgtgtat gccaagaata cgtcggtatc cccaaaagat ccgaaaacat
     1261 tttccctttc agctcctaca gtatattcta cagatacgca tgtgcctact actacagaca
     1321 aatcataagg gtagtctaca gcagttatag gagtgaattc atcaacaata cctgaggaaa
     1381 ttgtctttat accgttattg taggccatgc atttatcacg gatatcaact tgttcaacat
     1441 gctcggattg ttccgcagcc tcatatatgt cagcaagcga gttgtattca tatattcctt
     1501 tcgtataggt attaacattt ttcaaaaatt cgttctccga agctacagca atggtagtat
     1561 atgctttttc cgcttcggat atgctgtttt ttgctgtcag ccatgtactt acgcttaaga
     1621 acataaatat accggagaga gttacaagca ctacaataag cattcccata acattcgacc
     1681 ttagcgactg tgtgatgctg taccatatta cggcaggcat gccaccttta attccttcac
     1741 ctttttttaa cctatatggt atctttgttt tctttttacc gccctttata gcaaaacata
     1801 atactgaaga tatacaaaga atcagaagaa gtattcccgt ctgaacggca aatatcataa
     1861 gaagcggggt aggaacagag tccagttttt cttgttcgat tgatgctgac gggagaggga
     1921 gtgaggaatc ggtatgtaat gcctttccgg tagaggcttt tgttgttaag tattcatttg
     1981 ttgtgtttat aacgcttcca tagaaggaca gagacataac tgcacttata gcaatggaga
     2041 taatagaaat gatgataacc gtaagaactg cacgcagtcg cactttttta tcagtgcttg
     2101 ctaaatttat aacaatggca tgcatagcca tagaaataat aaacactgca agaaccgccg
     2161 tagatatata tgtagtaatt ctgaagttat ctgacagagc gtaaagataa ggcaaggaag
     2221 gatcgttttt gtcataatag tagaatattc cgccatatcc cgccgttttc atctcagcct
     2281 caaaatcaga cgctttacct gttttgagaa tatatgtcct cgaatcgggt atgccggaaa
     2341 acctgtctcc gcccaaatct gtcagtgaag actgcaactt catagaatcg tttatagata
     2401 gttcaggcac aaataccgta ttgggagtaa aagtacaaca ttgggtattc catgcatcat
     2461 catccaaata aatacctgca acagtatagt tttcactatc tgataatgca ttcttattgc
     2521 catgcgattt aggagtccat gctgtttcca aaaaacttgt ctttaactca taaccggcat
     2581 tccataaact caattttaag acatcaccta ccttaatatt attactcttt gccagttggt
     2641 cacttatgag acaaacttta ttaccggagg aatagtcttg ttctgtaaaa gaaactcctt
     2701 gtgtaatata tgctctgccg gaggcaaaaa gaggaacact ttcaatattg tttacagtaa
     2761 ctatcttcac ggattctttt gctgttttct tgtttgctat tattgtgttc catgtgctat
     2821 tttcatcgga agaaagaaac tcttccaaag aattatctat ttttgcataa gaaagatatg
     2881 acaaatcagt at
//
LOCUS       DAOSOM010000088         2562 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_40367_length_2562_cov_5.303949, whole genome shotgun sequence.
ACCESSION   DAOSOM010000088 DAOSOM010000000
VERSION     DAOSOM010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40367_length_2562_cov_5.303949"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1195)
                     /gene="hflX"
                     /locus_tag="PLF14_10010"
     CDS             complement(<1..1195)
                     /gene="hflX"
                     /locus_tag="PLF14_10010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOT71652.1"
                     /translation="MEKIVYGNTEGLKRSTLDILLSIYDDYDGSGYYCTQDTLDKICS
                     VSSDSKREIAVYINKRNRVEMVAVGDSELVRTDYRPERGMRLIHTHPSGSANLSAVDM
                     ETLRTSALSAMAAVSINRDGRMHMMYCACLDEYNQPVLFGPYKKLSQTQSFMDILKDT
                     YNTKNDATEITRKESERVVAVGLNSAYSAPLMEELAALISAAGGTCVASVVQNKDQPD
                     PAYYIGKGKIIELLHTCARYQADTVVFDDELTVSQQSNIEAELNVKVLDRTAVILDIF
                     AKRAFTNEGKLQVELAMLGYIYPRLTGKGQVLSRLGGGIGTRGPGETKLETDRRHIRR
                     RMTYLKEQLNKVDKRRTLLRDNKSRKSFFTFAIAGYTNAGKSTLLNALTDSDVMAEDM
                     LFATLD"
     gene            complement(1195..2532)
                     /locus_tag="PLF14_10015"
     CDS             complement(1195..2532)
                     /locus_tag="PLF14_10015"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOT71653.1"
                     /translation="MKQLIAKLFEQGRLMGFTDMEVLTNINESFEVKIYGQQIDSYKV
                     TDSRGMGFRGVYDNKMGYSYTEKLSEDSIDILLEDARQNAIINDNPDQDVIFEGSKEY
                     KKVENWFPALEKVTVEEKIRFAKDMERLALSVDSRIKSCPYCVFGSSSGSTAMVNSKG
                     LDLSDKGNAVFCYISVLAQDGQDYSSGVAFDFSSDFSELNPENLAKEAAEKALSYLGV
                     GPVPTGTYKAVMLNDASADILGAVSSCFSARAVQKGMSFLKGKLGTKVASEAVTIVDD
                     PFLPGMSGTGSFDGEGVATKYKEVIKDGVLKTYLHNLKTAKIDKTESTGNAGRSYKSN
                     IGVAPTNMYFAKGNKTFDKLLETMDEGIVITEVSALHSGFNDISGDFSLPAKGYTVKN
                     GKKDKYFRQITISGNYFKMLTDITEVGDDLKFATDGKIGSPSLLISEISVAGA"
BASE COUNT          622 a          641 c          481 g          818 t
ORIGIN      
        1 gatccaatgt ggcaaacagc atatcttccg ccattacatc gctgtctgta agtgcattta
       61 acaaggtgga tttaccggca ttggtatagc cagcaatggc aaatgtgaaa aagctctttc
      121 tggacttatt atctctgaga agggttcttc ttttatccac tttattgagc tgttctttta
      181 agtaagtcat cctcctcctt atatgtcttc tgtcggtttc cagtttggtt tctcccggtc
      241 ctctggtgcc tataccgcct cccagtctcg aaagaacctg tcctttaccg gtcagtctgg
      301 ggtaaatata acccagcata gccaattcca cctgcagttt cccttcgttg gtaaaagctc
      361 tttttgcaaa tatgtccaat attacagccg ttctgtccag cactttgaca ttgagttccg
      421 cctctatatt gctctgctgc gaaacggtaa gttcgtcatc gaaaacaacg gtatcggcct
      481 gatagcgtgc acaggtatgc agcaattcga ttattttacc tttgcctatg taataagcgg
      541 gatccggctg gtctttattc tgcactaccg aagccacgca tgttccgcct gctgccgaaa
      601 tcagtgccgc cagttcttcc ataagcgggg cggaataagc ggaattaagc cctaccgcaa
      661 ccactctctc actttcttta cgggttattt ccgttgcatc gttttttgta ttataggtgt
      721 ctttcaatat atccatgaaa gactgtgtct gagataactt cttatacgga ccgaacagca
      781 caggttgatt atattcatca agacatgcac aatacatcat gtgcatcctg ccgtccctgt
      841 taatactaac cgcagccatg gcggaaagtg cggaagttcg taaagtctcc atatcaacag
      901 ccgacaggtt ggcacttccg gacggatgag tatgaataag tctcatgcct ctttccggcc
      961 ggtaatcggt tcttaccaac tccgagtcgc ccaccgcaac catctcaact ctgtttcttt
     1021 tattgatata taccgctatt tctcttttgc tatcggaaga caccgaacaa atcttgtcca
     1081 atgtatcttg cgtacagtaa tagcccgaac cgtcataatc atcatatatg ctcaacaata
     1141 tatctaatgt gcttcttttt aaaccttcgg tatttccgta aacaatcttc tccattatgc
     1201 tcccgctacg cttatttccg atatcaacag cgacggagac cctatttttc catcggtagc
     1261 gaatttaagg tcatcgccta cctcggttat atcggtcagc atcttaaagt aattgccgga
     1321 tatggttatc tgcctgaaat atttatcttt tttaccgttt tttacggtat atccttttgc
     1381 cggtaatgaa aaatcaccgc ttatgtcatt aaatcccgaa tgtaaggcgg aaacttccgt
     1441 tattacaatg ccttcgtcca ttgtttccag aagcttgtca aatgtcttgt taccttttgc
     1501 aaaatacata tttgtgggag ctacccctat gttgctcttg taggagcgac cggcatttcc
     1561 ggtactttcg gttttatcaa tcttagcggt tttcaggtta tgaagataag ttttaagcac
     1621 accgtctttt atcacttcct tgtatttggt tgccactcct tccccgtcaa aagaacctgt
     1681 tcccgacata cccggcagga aaggatcatc tactattgta accgcttcgg atgccacctt
     1741 tgttcctaat tttcctttga gaaaagacat gcctttttgt accgctcttg cggaaaagca
     1801 ggaactgaca gcccctaaaa tgtccgcaga tgcatcatta agcattaccg ctttatatgt
     1861 ccctgtcgga acaggcccca ccccgagata ggacagggct ttttccgccg cttccttggc
     1921 aagattttca ggattcaact cggaaaaatc gcttgagaaa tcgaaagcaa cgccgctgct
     1981 gtagtcctgt ccgtcctgtg caagaacgga aatatagcag aataccgcat tacctttgtc
     2041 ggacaaatcc aatcctttgc tgttaaccat tgcggtgctt cccgaactgg aaccgaaaac
     2101 gcagtaggga caggatttaa tcctgctgtc cacggacagt gcaagcctct ccatgtcttt
     2161 agcaaaccgt attttttctt ccaccgttac tttttccaat gcgggaaacc agttttcaac
     2221 ttttttatac tctttggaac cttcaaatat gacatcctga tcgggattgt cattgattat
     2281 ggcattctgc ctggcatcct caagcagtat atctatggaa tcttccgaaa gcttttcggt
     2341 ataggagtaa cccatcttgt tatcatacac tccccgaaag cccattcctc tgctgtccgt
     2401 aaccttatag ctgtctatct gttgcccgta tatttttact tcaaagcttt catttatatt
     2461 cgtaaggact tccatatcgg taaatcccat aagtctgccc tgttcgaaaa gttttgctat
     2521 taactgtttc attgtattat cttcctccta cggtgatttt tg
//
LOCUS       DAOSOM010000089         2362 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_45092_length_2362_cov_10.666667, whole genome shotgun
            sequence.
ACCESSION   DAOSOM010000089 DAOSOM010000000
VERSION     DAOSOM010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45092_length_2362_cov_10.666667"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2362)
                     /locus_tag="PLF14_10020"
     CDS             complement(<1..>2362)
                     /locus_tag="PLF14_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71654.1"
                     /translation="MVFIHEAILTPITRPEVSNESFDRFGGFLPNVSRMKAQDTYELV
                     TGDTPAGEALTLTVEAALKQQKQGDVLDKVVGVYIVDAKKFETDDKSREGEYAEITEL
                     PDPGCPVAMREWLRNLNNRSTGLENVFFKYFDAESITQRDDDSGMADFKGEIMLYNLP
                     AGPFKPVFIVVTEKGAYDVYAPEIDEDKQLVGLRKSGFLSFFIAAADMYAVAQTGPAV
                     PLHFILPLLEITPALSEAGEMLEFDATIDIDDYNQGYLNYEYSIGVNCDAGTSTPGGA
                     PGSATSYLPAKLSLEVGGSISTSVSGKNLTIEDKFTAKTGASADTDKYLPAFIKDLAT
                     MEVKATAQAFSTAAIEYKNDAEIKSHENSIGVTGELETKTNVSGVEAAGKFLAGGVLA
                     PVFKVIKKWGIDIGGCIDANAGIGATVKYGLDAYTDGGNLNSIAADIDFGVAAKLGIY
                     GNVCGGKYGGARGTLGFGGSHSNFDSSMMTLTLKFVYDAKENEFRMEPAELIGMCEGD
                     FEAFLKTPVKDFNIKWHFSVFDVNMQFGTETKFTLTPIGKVYREVDRETFGTSTFMAD
                     EASGIMVKDYLSLATYATDENSGNILYTDLTSQGGDMRLQLSNYSEGIWKQPITILTT
                     DGTLGSYDLLTLPNGKILITYNLISKGHMNSMFPDSETKYTLGSIGANGWVEEASGTI
                     EQLAGSYAFSCDLAGSYLVCRLTGGTGAEGFKIVGYKWNGAGFGAAQVLGYAATYDMS
                     VAATDNELVVTCIDEWKELQAISWTGDVTNTTIAEDVGLVLAMTSDA"
BASE COUNT          585 a          574 c          464 g          739 t
ORIGIN      
        1 ctgcatcaga tgtcatggct aaaactaagc ctacatcctc tgctatcgtt gtgtttgtta
       61 catcacctgt ccagctgatt gcctgcagtt ccttccattc gtcaatgcag gtaactacca
      121 attcgttatc ggttgccgca acgctcatgt cataagtagc ggcatatccc agtacttgtg
      181 cagcaccgaa gcctgctccg ttccatttgt aacctactat cttgaagcct tccgctcctg
      241 taccgcctgt taaacggcag actaagtaag atcctgccaa atcacagctg aaagcgtatg
      301 agcctgctaa ttgttctatt gttcctgagg cttcttctac ccatccgttt gcacctatgc
      361 ttcccaatgt atatttggtt tcggaatcgg ggaacatact gttcatatgt cctttagata
      421 ttaagttgta tgttatcaat atcttaccgt taggtaatgt caataaatca taactgccta
      481 atgttccgtc ggttgtaagt atcgttatcg gttgcttcca gattccttca ctgtagttac
      541 tgagctgtaa cctcatatct ccgccttggc ttgtaaggtc ggtgtagagg atgttaccgc
      601 tgttttcatc tgtggcataa gttgcaagac tgagatagtc ttttaccatt atgcctgatg
      661 cttcatcagc catgaaggtg gatgttccga aggtttcacg gtctacctct ctatatactt
      721 tacctatcgg tgtaagcgta aacttggttt ccgtaccgaa ctgcatgtta acatcaaata
      781 cagagaaatg ccatttaata ttaaagtctt taacgggtgt ttttaagaag gcttcaaaat
      841 caccttcgca cataccgata agttccgcag gttccatacg gaattcattc tctttggcat
      901 catagacgaa ctttaatgta agtgtcatca tggagctgtc gaagttggaa tggctgccgc
      961 cgaaacctaa ggttcctctg gcaccgccat acttacctcc gcaaacattg ccgtaaattc
     1021 ctaacttagc cgctacaccg aagtctatat cagctgctat tgaatttaag tttccgccgt
     1081 cggtatatgc atccaagccg tatttgacgg ttgcccctat acctgcattt gcatctatac
     1141 aaccgcctat atcaataccc cattttttaa ttaccttgaa tacaggtgcc aatacgcccc
     1201 cggctaagaa tttgccggca gcttctaccc cgctgacatt ggtcttggtt tccaattctc
     1261 cggtaacacc tatgctgttt tcatgggact taatctcggc atcgtttttg tattctatag
     1321 cggctgtgga gaaagcttgt gcagtagcct ttacttccat agtagctaag tcttttatga
     1381 atgccggcag gtatttatcg gtatcggctg atgctccggt tttagcggta aatttatctt
     1441 caatagttaa atttttaccc gataccgatg tgcttatcga gcctcccact tccaatgaca
     1501 acttggccgg cagataagat gtggccgaac ccggagctcc tccgggagtg gatgttcccg
     1561 catcgcagtt aacacctata ctgtattcat aattaagata tccctggttg tagtcatcta
     1621 tatctattgt agcatcaaat tccaacatct ctcccgcttc actaagagca ggtgtaattt
     1681 ccagtaaagg aagtatgaag tgtagcggta cggcaggtcc ggtttgtgct accgcataca
     1741 tatccgctgc agctatgaag aaggaaagaa atccgctctt tcttaagcct acaagttgct
     1801 tatcttcatc gatttccggt gcataaacat cataggcacc tttttccgtt acaactatga
     1861 ataccggttt gaagggacct gcaggaagat tgtacaacat aatctcgcct ttaaagtcgg
     1921 ccattccgga atcgtcatct ctctgagtaa tgctttccgc atcgaagtat ttaaagaata
     1981 cattttccag accggttgaa cggttattaa gatttctaag ccattctctc atagcaaccg
     2041 gacatcccgg gtcgggtaat tccgttatct cggcatactc cccttcacgg gatttatcat
     2101 cagtctcaaa tttcttggca tctacaatgt atacgcccac aaccttatcc aaaacatcac
     2161 cttgtttctg ttgtttcaaa gcggcttcca cagtcagtgt aagagcttcg cctgcaggtg
     2221 tatctcccgt aacaagctca tatgtgtctt gtgctttcat acgactgaca ttgggtaaaa
     2281 aacctccgaa acggtcaaag ctttcattgg aaacttccgg tcttgttatc ggtgttaata
     2341 ttgcttcgtg tataaagacc at
//
LOCUS       DAOSOM010000090         2285 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_47147_length_2285_cov_15.207175, whole genome shotgun
            sequence.
ACCESSION   DAOSOM010000090 DAOSOM010000000
VERSION     DAOSOM010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47147_length_2285_cov_15.207175"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..>2285)
                     /locus_tag="PLF14_10025"
     CDS             complement(78..>2285)
                     /locus_tag="PLF14_10025"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOT71655.1"
                     /translation="NTTIAEDVGLVLAMTSDAGGYYLVYENGGAVDFYTRGINPGSAW
                     TKKGTPVSNVSAIEMDLAVNDDKLVLSFIKPSEAHFDDNNKLVVGNEGIASVTLSKDG
                     TLYDEQRTYLENSQFYRNLSIVCVNGLAYAFAEDLYEDTADLVFVKIYDADAPVNFFG
                     RVTITGTERFNEQLTAAVSEDNSVTKNFTYTWKRDGAAIATGATYTTVAADIGKTLVC
                     EVKTDDHQGVIRKATGVIAKAAGPAAPAGLAGVAPTVAGGNDGKITGTNQTMEFSQTA
                     TGVYTACRGVEITGLTAGTYYVRIAGNNTTEAGAYRAVTVGAGAQQPDDPPIPDDPVI
                     PDDPVIPDAGTGQDTSKTIPSTDGDTQVDYTETKGTVSLDISTDKAEEIINKSKDGDV
                     GFDLSGVKDAKSAEMSKEALETFGKADVSVTVKLPEGSVTMDKEAVDSIAEQAGGDTV
                     SVELALVKPEELNYLQEQSVGADDLIIDANITGGDGKKISTFNGELIMRIPYNGPLPV
                     AVWYLNDAGELEKIDCDYENGIVTFRLNHLSVYILGQDVSRERDWTNPFTDVNKNAWY
                     YSAIEYINKNKLMYGVGDNQFDPHGHMVRAMIVTVLHRLEKMPEPKAYNTFGDVAANR
                     YYSKAVNWAAENGIVNGYGDGNFGPNDPVTREQLAVIFMNYAKYKGYDTSKRAALETF
                     ADAKSISGWATDAMAWANAEALILGDGTGLNPSGKGERCHAAAIFQRFIANIK"
BASE COUNT          515 a          579 c          457 g          734 t
ORIGIN      
        1 acaaagcaaa ggtaccacac ctttggcggc ggcatggtac ccttgttgtt gaggaagatt
       61 atttagaata ttacctctta ctttatgttg gcaataaacc tctggaatat tgccgctgca
      121 tgacagcgct ctccttttcc tgaaggattc aagcctgtgc cgtcacccaa tatgagagct
      181 tcggcgttgg cccatgccat ggcatccgtt gcccagccgg atatactctt tgcatctgca
      241 aatgtttcca gtgcggctct cttggatgtg tcatagcctt tgtacttggc atagttcata
      301 aagattactg ccagctgctc tcttgtaacg ggatcgttgg gaccgaagtt tccgtcaccg
      361 taaccgttta ctataccgtt ctctgcagcc cagttgacag ctttgctgta gtatctgttt
      421 gccgcaacat caccgaatgt gttgtatgct ttcggttcgg gcatcttctc caagcgatgt
      481 aataccgtta ctatcattgc tcttaccata tgtccgtgag gatcaaattg attatcacct
      541 acaccgtaca tcagtttgtt cttgtttatg tactctattg cactgtagta ccaggcattt
      601 ttgttaacat cggtaaacgg attggtccag tctctttctc tgcttacatc ctgtcctaat
      661 atgtataccg aaaggtggtt gagacggaat gtaactatac cgttttcgta atcacaatct
      721 atcttttcca gctctcccgc atcatttaag taccagaccg ctacaggcag aggaccgttg
      781 taaggtatac gcattatcag ttcaccgttg aaggtactta tcttcttacc gtctccgccg
      841 gtaatattgg catcgataat cagatcatca gctcctaccg actgttcctg cagatagtta
      901 agttcttccg gttttaccag tgcaagttcc acacttaccg tatctcctcc tgcctgttcc
      961 gcaatggaat ctacggcttc tttatccata gttaccgatc cttcgggcaa cttaacagtt
     1021 accgatacat cggctttgcc gaatgtttcc aatgcctctt tagacatttc agcagacttg
     1081 gcatctttaa caccggaaag gtcaaatcct acatctccgt ctttgctctt gttgataatc
     1141 tcttcagcct tgtcagttga tatatccagt gataccgtac ccttggtttc agtgtaatcc
     1201 acctgggtat caccgtcggt tgagggaatg gtctttgatg tatcctgtcc cgttccggca
     1261 tccggaataa cagggtcgtc aggaatgacc gggtcatcgg gaatcggagg atcatccggt
     1321 tgttgtgctc cggctcctac cgtaacagca cgatatgctc ccgcctctgt ggtattgtta
     1381 cctgcaattc tgacatagta tgttcccgca gtaagtcccg ttatctcaac tcctctacaa
     1441 gcagtatata ctcctgttgc cgtttggctg aattccatgg tttgatttgt tcctgtaatc
     1501 ttaccgtcat tacctccggc aacggtaggt gctacacctg caagtcctgc gggtgccgca
     1561 ggtccggccg ctttggctat tacacctgtt gcttttctta tcacaccctg gtgatcatcc
     1621 gttttaactt cgcatacaag tgtctttcct atgtcggcag ctaccgttgt atatgtagct
     1681 cctgtcgcta ttgctgctcc gtcacgtttc catgtgtaag taaagttctt tgttacactg
     1741 ttatcttcac ttacagctgc cgtaagttgc tcattaaatc tttctgttcc cgttatggta
     1801 actctgccga agaagttaac aggtgcatcg gcatcgtaaa tcttaacaaa taccaaatcc
     1861 gccgtatctt catacaaatc ttcggcaaat gcataagcca gtccgttgac acatacaata
     1921 ctaagatttc tgtagaactg actgttttcc aaatacgttc tctgttcatc atacagagtt
     1981 ccgtctttgc taagtgtaac acttgcaata ccttcgtttc ctactaccag tttgttattg
     2041 tcatcaaagt gtgcttcgga aggctttatg aaacttaaaa caagtttgtc atcgttaact
     2101 gcaaggtcca tttcaattgc acttacattg gaaacggggg tacccttctt tgtccatgca
     2161 gagccgggat taatacctct tgtatagaag tccactgctc cgccattttc gtaaacaagg
     2221 tagtatccgc ctgcatcaga tgtcatggct aaaactaagc ctacatcctc tgctatcgtt
     2281 gtgtt
//
LOCUS       DAOSOM010000091         2174 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_50455_length_2174_cov_5.954696, whole genome shotgun sequence.
ACCESSION   DAOSOM010000091 DAOSOM010000000
VERSION     DAOSOM010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50455_length_2174_cov_5.954696"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1623)
                     /locus_tag="PLF14_10030"
     CDS             complement(<1..1623)
                     /locus_tag="PLF14_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009326181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOT71656.1"
                     /translation="MNVYEVYTQKVIDDKASLLFKEERSLKRLDGWFLAREIVCDTHE
                     ELKDLPKELRAAYEIKNIITNIPLGISENNIFAGTQRDAFARSYALINPSFRVETFSG
                     YCDPTAIYDDIEPNEEFTKERIAKVKEFTKNTEFVKKLTKVYDDYEQYTKEVIFFVEQ
                     VTGHVIPDFRPALKYGVKAIIENTEERIKNEENEQKRNNLAAMKIALECVILLANRYA
                     DIAASQYKEACAERKKQLALLESTLRKVPALPSENLYEAIQAYLLLWQVMCLEQAPNP
                     FAFSVGNADRIFEPYRKNFTREQAASLFRHFLVFYNVGDRSWAISQNVLLGGKSNSGV
                     DLTNVCTYAFLDAYYAMNFPQPILSVKLHKNTPQKLYEEMGRFFFTPGCLTPSFFNDD
                     SVFQVLKKAGIEETDLEDYSVAGCQEPLIMGKDNGNTTNSWLNLGKILEMTINNGRSL
                     ISGEQLGRKRNVDNLNILGNIKEYFYDDAKYYIKHMADAANGASVALSELPVPFLSSF
                     MGGLETGYDMRDVKNQGTRYNASGCLIHGLSVV"
     gene            complement(1639..>2174)
                     /locus_tag="PLF14_10035"
     CDS             complement(1639..>2174)
                     /locus_tag="PLF14_10035"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT71657.1"
                     /translation="MFFSGGGVTFTGGEPTLNLPALIDMLKKLKQEDINTAIETNGSH
                     PDLASATEYIDHLIIDFKHYDDDRHRKTVGISNAIIKKNLKRLSLIRKINIRIPLIKG
                     FNAEEENAVGFVNTLSECNKQNITVEFLSFHEFGKSKWQQCGMEYKMKGYEYISDDTS
                     EMFTRIFKDNGFDIIRT"
BASE COUNT          552 a          478 c          426 g          718 t
ORIGIN      
        1 gacaaccgat aaaccgtgaa taaggcatcc gcttgcattg tacctggttc cctgattttt
       61 aacatccctc atgtcatagc cggtttccaa gccgcccata aagcttgata agaaaggtac
      121 cggcagttcc gataaagcaa ccgaagcgcc gtttgccgca tccgccatat gttttatata
      181 atacttagca tcatcgtaaa agtactcttt tatattcccc agtatattca gattatcaac
      241 atttctcttt cttcccagct gctcgccaga tataagggag cgtccgttgt ttattgtcat
      301 ttccagtatc ttaccgaggt tcagccagct gttggtggta ttgccgttat ctttacccat
      361 aatcaacggt tcctgacagc ctgctaccga gtaatcttcc aggtcggttt cttcaatacc
      421 cgcttttttt agcacttgga aaaccgagtc gtcattgaaa aaagaaggag taagacaccc
      481 gggcgtaaag aaaaaccttc ccatctcttc atacagtttt tgaggggtgt tcttatgcag
      541 tttgaccgat aatatgggtt gcgggaagtt catggcatag tatgcgtcca ggaacgcata
      601 tgtgcagaca ttggtaaggt cgacaccgga attggatttg cctcctaaga gtacattctg
      661 cgatatggcc caggatcggt ctcccacatt atagaataca aggaaatgcc tgaataaact
      721 tgccgcctgc tctctggtaa aatttttcct gtaaggctcg aatatcctgt ccgcatttcc
      781 tacggaaaag gcaaacggat tgggagcctg ctcaaggcac attacctgcc ataataacag
      841 gtatgcctgt attgcctcat atagattttc cgacggcagg gcaggaactt ttctcagtgt
      901 ggattccaaa agcgcaagct gcttttttct ttcggcacaa gcttctttgt attgcgatgc
      961 cgcaatatcg gcatatctgt tagccaacag aattacgcat tcaagagcaa tcttcattgc
     1021 cgcaaggtta tttcgtttct gctcgttttc ttcgtttttt atacgctctt ccgtattttc
     1081 tattatggct tttacaccgt attttaaagc cggacggaaa tccggtatca catgtcccgt
     1141 aacctgttcc acaaagaata ttacttcctt tgtgtattgt tcgtagtcat catagacttt
     1201 tgtaagtttc tttacaaatt ccgtattctt ggtaaattcc ttaactttgg caatgcgttc
     1261 ttttgtaaat tcttcgttag gttcaatatc atcatatatc gctgtggggt cgcagtaacc
     1321 ggagaatgtt tccacccgga atgagggatt tatgagggca taggacctgg caaatgcatc
     1381 tctttgcgtt cctgcaaaga tattgttttc gcttatgccc agcggtatgt ttgtgataat
     1441 gtttttaatt tcataagctg ctcgaagttc cttgggtaaa tctttcaatt cttcatgtgt
     1501 atcgcaaaca atctcacgag caagaaacca accgtccagt ctttttaaac tgcgctcttc
     1561 tttgaaaagc agggaggcct tgtcatctat tactttttgt gtgtaaactt catatacatt
     1621 catgtctacc tccgaaagct atgtccttat tatatcaaat ccgttatctt taaatatacg
     1681 ggtaaacatt tccgaagtgt catcggaaat atattcatag cctttcattt tatactccat
     1741 accgcattgt tgccatttgg atttgccgaa ttcatgaaat gacaaaaatt caacggttat
     1801 gttctgcttg ttacattccg ataaggtatt tacaaacccc acggcatttt cctcttccgc
     1861 attaaaaccc tttatgagcg gtattcgtat gttgattttt cttataagcg ataatctttt
     1921 caggtttttc ttaattattg cattggagat acccacggtt tttctgtgtc tgtcatcatc
     1981 atagtgttta aagtcgataa tgagatggtc gatatattcc gttgccgatg caagatcggg
     2041 atgggaaccg ttggtctcta tagcggtatt aatatcttcc tgtttcaact tttttagcat
     2101 gtcaatcaaa gcaggcagat ttaatgtcgg ttctcctccg gtaaaggtaa cgccgcctcc
     2161 ggaaaagaac atgg
//
LOCUS       DAOSOM010000092         1925 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_59671_length_1925_cov_4.225134, whole genome shotgun sequence.
ACCESSION   DAOSOM010000092 DAOSOM010000000
VERSION     DAOSOM010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59671_length_1925_cov_4.225134"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..1868)
                     /locus_tag="PLF14_10040"
     CDS             complement(198..1868)
                     /locus_tag="PLF14_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOT71658.1"
                     /translation="MNNRQSYTAVKNNDKITALYCRLSRDDELQGDSNSIKNQKAILQ
                     KYADDNGFANTEFFVDDGYSGTNFDRPDWQRLLSQVEEGNIGTVIVKDMSRLGRDYLK
                     VGYYTEVLFPGSDIRFIAINNNVDSANQQDSDFTPFLNIINEWYAKDTSKKIRSVFKS
                     KGQSGKPLCTNPPYGYIKDPEDKMHWIVDEEAAKVVKEAFHLCMQGYGPTQIAKEFSK
                     RKIMNPTAHAKASGINIPDNRGHDDDYVWRGSTIVHMLSRQEYLGHTVNFKTYRKSYK
                     QKKQMKNDPSEWMIFENTHEAIIEESVFEVVQKIRDGRRRLTPMGEMPILSGMLFCAD
                     CGNKLYQVRHRGWEHDKEHFVCATYRKIKGCCSSHQIKNVVVEELLLDGIHRVTAFAR
                     DHEDEFVEMVTKKTRIELDKSMRDGKRELEQSQARIKKLDEIIQRLYEDNIEGKISDE
                     RFAKMTANYEAEQQNLESRVAELKSTMTAEKESALGVEHFFALVRKYTDIKELTAEII
                     REFVERIYVYKAERIDGRRVQRIKIIYNCIGDFDPSVSISTTEQEKSA"
BASE COUNT          474 a          432 c          376 g          643 t
ORIGIN      
        1 gcaatacggc aggatatata aatgattatg aagtatttga ttatctttac tcgtcatctg
       61 agtacctaat agttgaaata gacagagaga atcttacagt gagtgtacga tggacaaaat
      121 aaaacccgct ttagagggtg cgtcttaggg atttttaatc ccggaaaaat atcggcatag
      181 tttggtttta ttaccggcta tgccgatttt tcttgttctg tggtggatat agaaaccgat
      241 gggtcaaaat cgccaataca gttataaatg attttgatac gttgtactcg tctgccatca
      301 atgcgctcag ccttataaac atagattctt tcaacaaatt cccggataat ctccgctgtg
      361 agttccttta tatctgtgta ctttcttacc aaggcgaaga agtgttctac tcccagtgca
      421 ctttcctttt ctgcggtcat ggtggatttc agttctgcca ctcgactttc aaggttttgt
      481 tgctcggctt catagtttgc agtcatcttt gcaaagcgct catcggatat tttcccctca
      541 atattatcct cataaagcct ttggataatt tcatccagct ttttgatacg agcctgtgat
      601 tgctctaatt ctcgttttcc gtcacgcata ctcttgtcaa gttctatcct tgttttcttc
      661 gtgaccatct ccacaaattc atcctcatgg tctctggcaa aagcggttac ccggtgaatg
      721 ccgtcaagga gcaattcctc aactacaaca ttttttattt ggtgagaact gcagcagcct
      781 tttattttac ggtaggtagc acacacaaaa tgctccttat catgctccca acctctgtgg
      841 cgtacttggt ataacttgtt gccgcagtct gcgcagaata gcataccgga gaggattggc
      901 atttcaccca ttggtgtaag cctgcgtctg ccatcccgga ttttctgcac tacctcgaat
      961 acagactctt caataatcgc ttcatgggta ttctcaaaaa tcatccattc tgatgggtca
     1021 ttcttcatct gctttttctg cttataagac ttacggtaag ttttgaaatt tactgtatgt
     1081 cctaaatact cctgccttga aagcatatga acgattgtac tgcctcgcca gacataatca
     1141 tcgtcatggc ctctattgtc cgggatatta atcccgcttg cctttgcgtg ggcagttgga
     1201 ttcattatct tccttttgct gaattctttg gctatctgcg tagggccata accttgcata
     1261 cataggtgaa acgcctcttt taccacttta gcagcttctt catcaactat ccaatgcatc
     1321 ttatcttccg ggtctttaat gtagccataa ggtggattgg tgcagagcgg cttgccggat
     1381 tgaccttttg acttaaaaac ggatcgaatt ttcttgctcg tatccttagc ataccattcg
     1441 ttgatgatat taaggaatgg tgtaaaatcg ctgtcttgct gattggcact gtccacattg
     1501 ttgtttattg ctatgaaacg gatatcagag ccaggaaaca atacttcggt gtagtaacct
     1561 actttaagat aatctctccc aagacggctc atgtccttta caataacggt gccgatgttg
     1621 ccttcttcca cttgggataa aaggcgctgc caatccggtc ggtcaaagtt cgtaccgcta
     1681 tagccatcat ccacaaagaa ctctgtgttt gcaaagccgt tgtcatctgc gtacttctgt
     1741 aaaatggcct tctggttttt aatgctgtta gagtcaccct gcaactcgtc atctcgtgag
     1801 agacggcagt acagagcggt tattttatcg ttgttcttca cagcagtata agactgtctg
     1861 ttattcattt ttacctcgta taataaaaag tagtagttat taaagttcct ctccaagaac
     1921 tttga
//
LOCUS       DAOSOM010000093         1686 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_71939_length_1686_cov_2.804414, whole genome shotgun sequence.
ACCESSION   DAOSOM010000093 DAOSOM010000000
VERSION     DAOSOM010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71939_length_1686_cov_2.804414"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..>1686)
                     /locus_tag="PLF14_10045"
     CDS             complement(35..>1686)
                     /locus_tag="PLF14_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71659.1"
                     /translation="IFRDSIFQKKGRFAASSFNSDIVIKRMDTQQSLNMPYRYAKILL
                     DKKLQFADEIVGINVASVKYSMEENGISWYGYEDTFVKTDSEKDKKVVDSVKYCRGIS
                     ALSAIIADKEDSKIVIYDGYSEELLLKGYQNYVIIPSSMRKYVNKEIPDSEKILMMSK
                     SDSGSILPFTIIGEYKTEKKYDTLYVSYAGLSDLIRANQKDIPNHVDCLEIDVYEDKD
                     ITELVNYLSEYFAEGSVYSEYIGRLNVYREPYEYMFVHSLNIEPVVSLTNAIYGSNAI
                     TVSKIDGNSALEMSYVYADALIRDYDKYSQYISDIVISTGVTGINPYDYPMQPSQPGF
                     YDCPAYSTYLQYDFDILKEIKEKYSSGKYNYLYQAVTSVSEIYKVEKDCKVTFYLNYS
                     NKDMVVPRQKDITDIISGYAIVPFPMYEAACKLGIYNDKPVRLNESRAIYDELGNRTF
                     EGSTLAQFKIIGYYETSDEYDRIYVTYAGSNEKYKFEQFEKEYIEAITIIPKDGMDIS
                     PLIEYLEQYFSPANDTAKHMGQLNKLDMEYKYCFTIKESVD"
BASE COUNT          489 a          351 c          242 g          604 t
ORIGIN      
        1 ccccccgcat tttgtcaaca ttgtaaaaat atgattaatc aacactttcc tttattgtaa
       61 agcagtactt atactccata tccaatttat tcaactgccc catgtgtttt gcggtgtcat
      121 tcgcaggtga aaaatattgt tctaaatact ctatcagcgg tgaaatatcc ataccgtctt
      181 ttggtattat agttatggct tcaatatact ccttctcaaa ctgttcaaac ttgtatttct
      241 cattactgcc cgcatatgtt acataaatcc tgtcatattc gtcagatgtc tcgtaatagc
      301 cgattatttt aaattgggct aatgttgagc cttcaaaagt tctgttaccg agttcatcat
      361 aaattgctct ggattcattc aatcgaacag gtttatcgtt atatataccg agtttgcagg
      421 cagcttcata catcggaaac ggcactatcg cgtagccgct tattatatcc gttatatctt
      481 tttgtctcgg tacaaccata tccttattcg agtaatttaa ataaaatgtt actttacagt
      541 ctttttccac tttatatatt tcacttacac ttgttacagc ctgatacaag taattatact
      601 ttccggaact atacttttct tttatttctt tcaatatatc aaaatcatat tgtaaatatg
      661 tgctatatgc gggacaatca taaaatccgg gttgtgacgg ctgcatagga taatcgtacg
      721 gattaattcc tgttacacct gtacttatga caatatcaga tatgtactgt gagtatttat
      781 cgtaatctct aataagcgca tcagcatata catatgacat ctcaagagca ctgttgccgt
      841 caattttaga aacggttatt gcattacttc cgtaaatcgc attagtcaga gaaacaacag
      901 gctctatgtt caatgaatgg acaaacatat actcataagg ttctctataa acattcaatc
      961 tgccgatata ctcggaatat acacttcctt ccgcaaaata ttcgcttaga taattaacaa
     1021 gttccgtaat gtccttatcc tcatatacat caatctccag gcagtcaaca tggttgggta
     1081 tgtctttttg atttgcgcgg attaagtcgc ttaaacctgc atatgataca tacaaagtat
     1141 catacttctt ttccgtctta tactccccta tgatagtaaa aggcagtata ctgcctgagt
     1201 cgcttttgct catcatcaat atcttttcac tatcgggtat ttctttatta acatactttc
     1261 tcatggaaga tggaatgatt acatagtttt gataaccttt taacagtaat tcctccgaat
     1321 atccgtcata aatcactatc ttactgtctt ctttatctgc aattattgca cttaaagcgg
     1381 atatacccct gcaatacttc accgaatcta cgactttttt atctttttcg ctatcggttt
     1441 taacaaaagt atcctcatat ccgtaccaac tgatcccgtt ttcttccatg ctatatttaa
     1501 cggatgccac atttatacca acaatttcat ctgcaaattg cagcttttta tccaataaaa
     1561 tctttgcata tctgtatggc atattcagcg attgttgtgt atccattctt tttatgacaa
     1621 tatcgctgtt gaaagatgaa gcggcaaaac gacctttctt ttgaaatata ctgtcgcgga
     1681 aaataa
//
LOCUS       DAOSOM010000094         1675 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_72540_length_1675_cov_4.504938, whole genome shotgun sequence.
ACCESSION   DAOSOM010000094 DAOSOM010000000
VERSION     DAOSOM010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72540_length_1675_cov_4.504938"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(245..>1675)
                     /locus_tag="PLF14_10050"
     CDS             complement(245..>1675)
                     /locus_tag="PLF14_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71660.1"
                     /translation="LCFWGDDPYVKISSDAYVSSYLYQKSDAEDDISYILETGEGSFG
                     RGDLSYSPLLVLKYRDSVLIANQMDITDKINVIPQANHLFVSLLTELSYRNNRSIGKR
                     VNTYILDGDEYDSFEEIKENVKQGADLLINNLTDNNVKCWNDLIGTEIKLLKKNDIFN
                     CRIKKKDRIVNCISNYELNGINTYSYSKKDSKNYNLCDYVIIKNKNLVTVAETCQNSM
                     MKELFVYNGHTELRRAYTISKYFYSEQKKDEYDVISYMKYGKGKIYFNQFKNDIDNPK
                     HKRLLRRIEHNMLGSCSHSSFDYEKTESAKSSDGYPIYVNLSKVVINDCLWDRYVSTT
                     VNNNERMAHKKTISIGEWRLTELNSISSDENDILDDICYLITYNIFSSEPRKNIGSNL
                     GVPNPEDLTFLHVTGNGVIRLAVNGQDYGKKQCIDNLCVFSDISLEKGINYVMMAWFP
                     ERKDSNIKIEWKNINLKPESNLEFFN"
BASE COUNT          500 a          331 c          208 g          636 t
ORIGIN      
        1 aaaccaacga gcagacaagg attaaacaca acaccaaact taatacctta acttttttca
       61 taattttccc tcctcttttt aaaattataa atttactgga caggtttatt ataataagaa
      121 aaaaacaaat atgcaatgtg tattttacac atttttacaa tattctatgt ttttattaat
      181 aactttatac gatattatca gtatttttgc tatttgcata atgtcagatt gctaaatcac
      241 ttaattaatt gaaaaattcc agattgcttt ccggttttaa gttaatattc ttccattcaa
      301 tcttaatatt gctgtctttt ctttccggaa accatgccat cattacataa ttgatacctt
      361 tttccaaaga tatatcggaa aaaacacata aattatcaat acactgtttc tttccgtagt
      421 cctgtccgtt tactgccagc cttataaccc cgttgccggt tacatgaaga aatgttaagt
      481 cttccggatt gggtacaccc agattacttc cgatattctt tctcggttcg cttgaaaata
      541 tgttgtaggt tattaagtaa catatatcat ctaaaatatc attctcatca ctactgattg
      601 aattgagctc cgttaaccgc cattcaccga tacttattgt ctttttgtgt gccattcttt
      661 cattattgtt tacggtggtt gatacataac ggtcccatag acaatcatta atcacaacct
      721 tggataaatt aacatatatc ggataaccgt cactgctttt ggcactttcg gttttttcat
      781 aatcaaacga agaatgagag caactgccta acatgttatg ttcaatcctt cttaacaatc
      841 ttttgtgctt tggattgtca atgtcatttt taaattgatt aaagtatatc tttccttttc
      901 cgtacttcat atatgatatt acgtcatatt cgtctttttt ttgttccgag tagaagtatt
      961 ttgatattgt atatgctctt ctcagttccg tatgaccgtt gtatacaaac aattccttca
     1021 tcatgctgtt ttgacatgtt tccgcaacag tcactaaatt tttattcttt ataattacat
     1081 aatcacacag attatagttt ttggaatctt tcttactgta agaataagtg tttataccgt
     1141 tcagttcata atttgaaata cagttaacaa ttctatcctt cttttttatt ctgcaattga
     1201 atatatcatt ctttttcaat aatttaattt ccgtacctat aagatcgttc cagcacttta
     1261 cattattatc cgtaagattg tttatcagca gatccgctcc ttgttttaca ttttccttta
     1321 tttcttcaaa actgtcatat tcatccccat caagtatata tgtatttacc ctctttccta
     1381 tacttctatt gtttcggtac gacaactccg ttaaaagaga tacaaaaaga tgatttgcct
     1441 gcggtataac attaatctta tccgttatat ccatctgatt tgcaatcaat acggaatccc
     1501 tgtatttaag cactaaaaga ggcgagtaag ataaatctcc ccttccgaaa ctgccttcgc
     1561 ccgtttccaa tatatacgat atatcatcct ccgcatcgga tttttgatat aaatatgacg
     1621 atacatacgc atcacttgag atcttcacat aaggatcgtc accccagaaa cataa
//
LOCUS       DAOSOM010000095         1489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_85518_length_1489_cov_4.451185, whole genome shotgun sequence.
ACCESSION   DAOSOM010000095 DAOSOM010000000
VERSION     DAOSOM010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85518_length_1489_cov_4.451185"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="PLF14_10055"
     CDS             <1..493
                     /locus_tag="PLF14_10055"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HOT71661.1"
                     /translation="GNFLMNALFSKLNFKTVNYIDGTAEDVTKECMRYEELLNNNTID
                     IVCMGIGENGHIAFNDPPVADFSDKVKVKIVKLDELCRQQQVNDKCFENIHQVPKYAI
                     TLTIPALFEGKKLFCIVPAPTKANAVYDTLNGKIEEKCPASILRLHLAAKLYLDKDSA
                     SKL"
     gene            506..>1489
                     /locus_tag="PLF14_10060"
     CDS             506..>1489
                     /locus_tag="PLF14_10060"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HOT71662.1"
                     /translation="MNRETSVAGRFRYEGDIISIKPIGAGHINKTYVVETTCDKYTLQ
                     LINTTVFTNPVALMENIYNVTEFLKKKIAARGGDIRRETLSVVKTKDNKLFYVDSDNC
                     YWRSYLYIDDVTTYQQPKNLQVVRDSGYAFGDFLNMLSDYDMSTLHETIKDFHNTVKR
                     FRDFEMSVKRDVAGRVSSVSDEINFLYQRKADCGIILENIKSGKIPLRVTHNDTKLNN
                     LLFDNATDKPICILDLDTVMPGSILYDYGDALRISSSSAEEDEENLDKVHFVTDNFIY
                     FTKGFLAAVKNSINAVELSLLPFSIKLITMEIGMRFLTDYLNNDIYFAVHKE"
BASE COUNT          497 a          251 c          301 g          440 t
ORIGIN      
        1 cggtaatttt cttatgaatg ctcttttttc aaaattaaac tttaagacgg tcaactatat
       61 tgacggtacg gcggaagatg tgacaaaaga atgtatgcgt tatgaagaat tgttgaacaa
      121 taacactatt gatattgtct gtatgggaat aggggagaac ggacatatcg cttttaatga
      181 tcctccggtt gcggattttt ccgataaagt caaagttaaa atagtaaaac tggatgagtt
      241 atgtcgtcag cagcaggtta acgataagtg ctttgaaaat attcatcagg tacctaaata
      301 tgccattaca ttaacaatac cggctctttt tgagggaaaa aaacttttct gtattgtgcc
      361 ggcacctacc aaagcaaatg cggtttatga cactttaaac ggcaaaatag aggagaagtg
      421 tcctgcaagt atactgagac tgcatttagc tgcaaagctc tatttagaca aagacagcgc
      481 atctaagcta tagcaggagg agcatatgaa cagagaaact tccgttgcag gcagatttcg
      541 ttatgaggga gatattattt caataaaacc cataggtgcg ggtcatatta ataaaacata
      601 tgtagtggaa acaacatgcg ataaatatac ccttcaactt ataaacacaa cggtttttac
      661 caatcccgtc gctcttatgg aaaatatata taatgtgacg gagtttttaa aaaagaagat
      721 tgccgcacgg ggtggcgata taagaagaga aacgttatcc gttgtcaaaa caaaagataa
      781 taaattgttt tatgtggatt cggacaactg ttactggcgc tcatatttat acattgacga
      841 tgttacgaca tatcagcaac ctaagaatct tcaggtagta cgcgacagcg gctacgcatt
      901 cggtgatttt ttgaatatgc tttccgatta cgatatgagc actcttcatg aaacaattaa
      961 agactttcat aacacagtaa aacgattccg tgacttcgaa atgtccgtaa agagggatgt
     1021 tgcgggaaga gtctcatctg tttccgatga aattaacttt ttatatcaaa gaaaagccga
     1081 ttgtggaata atattggaga acataaagtc gggtaaaata cctttaagag ttacgcacaa
     1141 tgatacaaaa ctgaacaatc ttttatttga taatgcgacc gataaaccta tctgtatttt
     1201 ggatctggat accgtcatgc cgggctcaat actttacgat tacggtgatg cgttgagaat
     1261 ttcctcatct tccgctgagg aagatgaaga gaatcttgat aaagttcatt tcgttacgga
     1321 caacttcata tatttcacaa aaggtttttt agcagccgta aagaacagta ttaatgctgt
     1381 tgagttatca cttttgccgt tttccattaa acttataacc atggagatag gcatgagatt
     1441 tctcaccgat tatctgaata acgacatata ttttgcggta cacaaagaa
//
LOCUS       DAOSOM010000096         1418 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_91897_length_1418_cov_5.403522, whole genome shotgun sequence.
ACCESSION   DAOSOM010000096 DAOSOM010000000
VERSION     DAOSOM010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91897_length_1418_cov_5.403522"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(237..>1418)
                     /locus_tag="PLF14_10065"
     CDS             complement(237..>1418)
                     /locus_tag="PLF14_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71663.1"
                     /translation="INAFSVIIYIILSIVLPLLPMSTGSVIGFVVARISAKIKSRNII
                     MIAGSFALLALIMVFSFSLQNILDEQAITDMVTSIADIQSSFFLFDLYVDAVYGLNIL
                     SLLLFILIFTVPFILFIYLFAKSFKKINSSMMETKAHHSSKNVKFKEYSPVMAIFKKE
                     LNFYVHCVVYIVNTFFGMVLLLIGCIAVLVVNFDPESVFAEIAQAQGSLSVADIKLLA
                     AATVFGYCIMMSSPTSASISLEGRNLWILKSMPVETKDIFKGKTALGLVVTLPIGIIS
                     SVLIYIGLKLSFTQWLILLAFTVSAGFLTAVLGLFANILFPKMEFKSPTEVVKQSASV
                     AVAVFSGMFLVIIPMILGAALIMYNTSLFVLIVASIYAVITLILWRYLITKGVKRFAA
                     L"
BASE COUNT          506 a          275 c          242 g          395 t
ORIGIN      
        1 ctgtcgcgga aaataaagag catagccgca ataagaatca tgactactat cacaataaat
       61 actgctgtat atcttttttt ctttttatcc actgtaacct ccaaggcata aataaggctg
      121 aatcaattaa gtaaatattt cttttagttt atgataaagt ttcgtatatt tttgttttat
      181 atacactatc ttatcatact taaagatatg tgtttgatat aaagttctat ttgatactaa
      241 agtgctgcaa acctcttaac acccttggtt atgagatagc gccatagaat taaggttatg
      301 acggcatata ttgaagctac tatcaggaca aacaaagatg tattatacat tatcagtgca
      361 gcacccaaaa tcatgggaat tattactaaa aacatcccgg aaaaaaccgc cacagccacc
      421 gatgccgact gtttaacaac ttccgtcggg gatttgaatt ccattttcgg aaaaagaatg
      481 tttgcaaaca gacctaaaac cgctgtaaga aatcccgccg atacggtaaa tgcaaggaga
      541 ataagccact gtgtaaaaga cagttttaaa ccgatataga tgagcaccga cgatattatc
      601 cctataggca gagtaacaac aagtcctaaa gctgtttttc ctttaaaaat atcttttgtt
      661 tcaacgggca tggacttcaa tatccaaaga ttcctgcctt ccaaagatat ggaagcggag
      721 gtcggactgc tcatcataat acaataaccg aataccgtcg ctgccgccag aagctttata
      781 tctgcgacag ataaactgcc ttgtgcctgt gcaatttccg caaagaccga ttccggatca
      841 aaattcacaa ccaaaaccgc tatacaacct attaataaca gtaccatacc gaagaaagta
      901 tttactatat acacgacaca gtgaacatag aagttgagtt cctttttaaa tattgccatt
      961 accggagaat actctttgaa cttgacattc tttgatgagt gatgtgcctt tgtttccatc
     1021 atgcttgagt ttattttctt aaaagatttt gcgaacagat aaataaacag tatgaacgga
     1081 acggtgaata tgagaataaa cagaagcagc gataatatat tcagaccgta aaccgcatca
     1141 acatacaaat caaacaggaa aaaggatgac tgtatgtccg caatggaagt aaccatatcg
     1201 gtaatagcct gttcatccaa tatattctgt aaagaaaaag aaaaaaccat tataagggca
     1261 agtaatgcaa aagagcccgc aatcataatg atattcctgc ttttaatctt tgcggatatt
     1321 cttgccacaa caaaccctat cacggaacct gtactcatag gaagaagagg caacacaata
     1381 gaaagtatga tgtatatgat tacggaaaaa gcatttat
//
LOCUS       DAOSOM010000097         1025 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate AMR_MDS_2345
            NODE_150462_length_1025_cov_13.241237, whole genome shotgun
            sequence.
ACCESSION   DAOSOM010000097 DAOSOM010000000
VERSION     DAOSOM010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864412
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (wastewater metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillati; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:56:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,015
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150462_length_1025_cov_13.241237"
                     /isolate="AMR_MDS_2345"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="PLF14_10070"
     CDS             complement(<1..662)
                     /locus_tag="PLF14_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT71664.1"
                     /translation="METKQILLELDNAVYEYSNKYQTVRAVNEVSYEFHKGRFYAIVG
                     KSGCGKTTLLSLLAGLDVPKEGAVIYREKSTKDMDRDRYRREDVAVIYQDYNLFPLLT
                     AIENVMYPLQLRGMNEKQAMRKASEKIKSVGMDESFHKRFPSMLSGGEQQRIAIARAL
                     ASGAQIILADEPTGNLDSENGKNIIGTLKKLAHEEDYCVIVVTHDLEISADADEVLSM
                     RDG"
     gene            complement(676..>1025)
                     /locus_tag="PLF14_10075"
     CDS             complement(676..>1025)
                     /locus_tag="PLF14_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT71665.1"
                     /translation="SMLEKVQPILFILSAAIGFIACMLFIRNRKPEFANMRSMGTPKG
                     VVFFEAFFEQSLFGITGTLTGIAIYFLIHGKGALFIVEDILIFLVCYLAGATIAVINI
                     TRINVMKIMKAKE"
BASE COUNT          266 a          253 c          167 g          339 t
ORIGIN      
        1 ccgtctctca tacttaaaac ttcgtccgca tcagcggata tctccaagtc atgggtaacc
       61 acaattacac aatagtcttc ttcatgggca agctttttca gcgtcccaat tatattctta
      121 ccgttctcgg aatctaaatt accggtaggc tcgtctgcca atattatctg tgctcccgat
      181 gcaagagctc tggcaatggc tattctttgc tgctctcctc ccgaaagcat ggaaggaaat
      241 ctcttatgga agctttcatc catgcctacc gattttatct tttccgaagc tttccgcatt
      301 gcctgctttt cgttcattcc tctaagttgc agaggataca ttacattctc aatggcggta
      361 agaagaggaa acaggttata gtcctgatat attaccgcca catcttctct tctgtatctg
      421 tcacggtcca tatcttttgt ggacttctcc cgatatatta ccgctccttc tttaggcaca
      481 tccaaccctg caagaaggga tagaagagtg gttttaccgc aaccggactt gcccacaatg
      541 gcatagaatc tccctttatg aaactcgtaa cttacctcat ttaccgctct aacggtctga
      601 tatttgttac tgtactcata taccgcatta tccaactcca ataatatctg ttttgtttcc
      661 attgtttttt cctttctatt cctttgcttt cattattttc attacattaa ttcttgttat
      721 attaataacc gctattgtcg caccggcaag atagcatact aagaaaatga gtatgtcttc
      781 tacaatgaac agtgctcctt ttccgtggat taggaaatat atcgctatac ctgtcaaagt
      841 tcccgtaata ccgaacagtg attgctcaaa gaatgcttca aaaaagacca ctcctttggg
      901 cgtacccata cttctcatat tggcaaattc cggttttctg ttgcgtataa acagcataca
      961 tgcaataaac cctattgccg ctgacagtat aaacaagata ggttgcactt tttccagcat
     1021 actta
//
